LOCUS       DANRDI010000001        49587 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.001, whole genome shotgun sequence.
ACCESSION   DANRDI010000001 DANRDI010000000
VERSION     DANRDI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 49587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.001"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(100..1029)
                     /locus_tag="PZ377_000001"
     CDS             complement(100..1029)
                     /locus_tag="PZ377_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDM6356895.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(1271..2650)
                     /gene="argH"
                     /locus_tag="PZ377_000002"
     CDS             complement(1271..2650)
                     /gene="argH"
                     /locus_tag="PZ377_000002"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDM6356896.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(2640..3845)
                     /locus_tag="PZ377_000003"
     CDS             complement(2640..3845)
                     /locus_tag="PZ377_000003"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDM6356897.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            4196..5527
                     /locus_tag="PZ377_000004"
     CDS             4196..5527
                     /locus_tag="PZ377_000004"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDM6356898.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            5852..6427
                     /locus_tag="PZ377_000005"
     CDS             5852..6427
                     /locus_tag="PZ377_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDM6356899.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            6432..6956
                     /gene="lepB"
                     /locus_tag="PZ377_000006"
     CDS             6432..6956
                     /gene="lepB"
                     /locus_tag="PZ377_000006"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDM6356900.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            6972..7547
                     /gene="lepB"
                     /locus_tag="PZ377_000007"
     CDS             6972..7547
                     /gene="lepB"
                     /locus_tag="PZ377_000007"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDM6356901.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            7707..11183
                     /gene="addB"
                     /locus_tag="PZ377_000008"
     CDS             7707..11183
                     /gene="addB"
                     /locus_tag="PZ377_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDM6356902.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            11184..14837
                     /gene="addA"
                     /locus_tag="PZ377_000009"
     CDS             11184..14837
                     /gene="addA"
                     /locus_tag="PZ377_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDM6356903.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            15003..15905
                     /locus_tag="PZ377_000010"
     CDS             15003..15905
                     /locus_tag="PZ377_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDM6356904.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            16234..16623
                     /locus_tag="PZ377_000011"
     CDS             16234..16623
                     /locus_tag="PZ377_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDM6356905.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(16794..18110)
                     /locus_tag="PZ377_000012"
     CDS             complement(16794..18110)
                     /locus_tag="PZ377_000012"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDM6356906.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(18162..18986)
                     /locus_tag="PZ377_000013"
     CDS             complement(18162..18986)
                     /locus_tag="PZ377_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDM6356907.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            19100..19408
                     /locus_tag="PZ377_000014"
     CDS             19100..19408
                     /locus_tag="PZ377_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDM6356908.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            19955..21769
                     /locus_tag="PZ377_000015"
     CDS             19955..21769
                     /locus_tag="PZ377_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDM6356909.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            21972..24581
                     /gene="clpB"
                     /locus_tag="PZ377_000016"
     CDS             21972..24581
                     /gene="clpB"
                     /locus_tag="PZ377_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDM6356910.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(24640..25509)
                     /locus_tag="PZ377_000017"
     CDS             complement(24640..25509)
                     /locus_tag="PZ377_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDM6356911.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVF
                     IKSFKKYIANEQL"
     gene            25619..26764
                     /locus_tag="PZ377_000018"
     CDS             25619..26764
                     /locus_tag="PZ377_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDM6356912.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            26748..27389
                     /locus_tag="PZ377_000019"
     CDS             26748..27389
                     /locus_tag="PZ377_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HDM6356913.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            27382..28500
                     /locus_tag="PZ377_000020"
     CDS             27382..28500
                     /locus_tag="PZ377_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6356914.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRALILGFFIG
                     FIPINIYNDLKLNNLQFISVLTCYTVMGFVSSRYLTKYLNYKFVSEICLVIFLIIYTY
                     QSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGY
                     LYTIGTIQYLILFISLLIFIYLIIIFYFTRREQHENKN"
     gene            28484..28999
                     /locus_tag="PZ377_000021"
     CDS             28484..28999
                     /locus_tag="PZ377_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDM6356915.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            29310..29744
                     /locus_tag="PZ377_000022"
     CDS             29310..29744
                     /locus_tag="PZ377_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDM6356916.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(30001..30186)
                     /locus_tag="PZ377_000023"
     CDS             complement(30001..30186)
                     /locus_tag="PZ377_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDM6356917.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            30481..31422
                     /locus_tag="PZ377_000024"
     CDS             30481..31422
                     /locus_tag="PZ377_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDM6356918.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            31434..32678
                     /gene="fabF"
                     /locus_tag="PZ377_000025"
     CDS             31434..32678
                     /gene="fabF"
                     /locus_tag="PZ377_000025"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDM6356919.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(32733..33104)
                     /locus_tag="PZ377_000026"
     CDS             complement(32733..33104)
                     /locus_tag="PZ377_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDM6356920.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            33347..34273
                     /locus_tag="PZ377_000027"
     CDS             33347..34273
                     /locus_tag="PZ377_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6356921.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            34273..35343
                     /locus_tag="PZ377_000028"
     CDS             34273..35343
                     /locus_tag="PZ377_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6356922.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            35360..36442
                     /locus_tag="PZ377_000029"
     CDS             35360..36442
                     /locus_tag="PZ377_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6356923.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            36432..37373
                     /locus_tag="PZ377_000030"
     CDS             36432..37373
                     /locus_tag="PZ377_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDM6356924.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            37392..39047
                     /locus_tag="PZ377_000031"
     CDS             37392..39047
                     /locus_tag="PZ377_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDM6356925.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            39259..40974
                     /locus_tag="PZ377_000032"
     CDS             39259..40974
                     /locus_tag="PZ377_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDM6356926.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            41025..42011
                     /locus_tag="PZ377_000033"
     CDS             41025..42011
                     /locus_tag="PZ377_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6356927.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLETVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            42014..42994
                     /locus_tag="PZ377_000034"
     CDS             42014..42994
                     /locus_tag="PZ377_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDM6356928.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            42987..43949
                     /locus_tag="PZ377_000035"
     CDS             42987..43949
                     /locus_tag="PZ377_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6356929.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            43961..44842
                     /locus_tag="PZ377_000036"
     CDS             43961..44842
                     /locus_tag="PZ377_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6356930.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(44884..45873)
                     /gene="trpS"
                     /locus_tag="PZ377_000037"
     CDS             complement(44884..45873)
                     /gene="trpS"
                     /locus_tag="PZ377_000037"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDM6356931.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            46168..46563
                     /gene="spxA"
                     /locus_tag="PZ377_000038"
     CDS             46168..46563
                     /gene="spxA"
                     /locus_tag="PZ377_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDM6356932.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            46934..47653
                     /gene="mecA"
                     /locus_tag="PZ377_000039"
     CDS             46934..47653
                     /gene="mecA"
                     /locus_tag="PZ377_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDM6356933.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            47774..48759
                     /locus_tag="PZ377_000040"
                     /pseudo
     CDS             47774..48759
                     /locus_tag="PZ377_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            48807..>49587
                     /locus_tag="PZ377_000041"
     CDS             48807..>49587
                     /locus_tag="PZ377_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDM6356934.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREK"
BASE COUNT        17778 a         6898 c         8761 g        16150 t
ORIGIN      
        1 aaagacagca gtaggataat ttcaatttgg aaatatctta ctgctgtttt ttatagtaat
       61 agctatattt ttgtatttat gttttctagt ttaacattac tacttaatga cttctttata
      121 tttatcagcg aaatttgtaa agacaccatc aacgccatat ttatttaatc gtaacatatc
      181 agctttttca ttcactgtat aaggatgtac tataaatcct aagtctttta aatgatgggt
      241 attttgttca gttaaatctg tataatcagg acctaatcca atcgcataag agcgtatctc
      301 ttttaagcgt tggtcgttaa attgttgtag ttcaccttta tcaactaatt ttactaatgg
      361 cacatgctta ttttgacgat gaattttctt taaactttcg tcagaaaatg attgaatcat
      421 tacatgtcca ttttttaatt tattgttatt taaaaggtga tgctttttca atgaagctaa
      481 taattgttct tccattcctg ggtatacatc aggtgacttt gtttcaatat aatagtttgc
      541 attcgggcca taacgttcta aaatttcatc taaagtgggt actttagcat ttttataact
      601 tgctcttgcg tattttggat attttttatt aaaccaactt cctgcatcta actgttttaa
      661 ttcatcaagg gtataatcct caactttacc gtgtccattt gttgtacggt taacagtttc
      721 atcatgcata gcaactaaat ggccatcttt ggtacgttgt aaatcaattt cgatataaga
      781 tgcttttaac tcattatgac tcttatcata tgcttgaaac gtatgctcgg gtgcatagcc
      841 acttgcgcca cgatgtgcga tagtagtgaa tcgctcattc gttaaatttg tatgccattg
      901 aatagcctga ggtttatttg caatttgatt tgtttgttca gcgccagcag taggtactga
      961 taaaaatccc atagtaaaaa cagcagaagc agccataaat ttagtgaagc ttttcgaaga
     1021 gttagtcata gtctttatcc tttcatctct ttatttgtag tctaacaatt ttaaaatact
     1081 acaaaaacct gcttgtgaaa acgctttaca catttctttt acaatttata acatttttgt
     1141 aatataaatt taaaagaatt atcaaacatt ttaggaatct taaaaatgtg atgttcaaca
     1201 agacgaagag agcattaaaa tacccacaat gtaattttaa tagacattgt gggtaggtag
     1261 attaacgtat ttattgtgat agtaattgtt tagcaacatc aagttgttgt ttgaccgatg
     1321 attgacctgt tgaaccgtaa ctttgacgtc gttttaaaca attttcaggc tgcaaataat
     1381 cgtaaatatc ggcatcaata ctagaatgat gttgttgata tgttgctaaa ggaacatcta
     1441 ataaataatg accttgttgt atacattcta agacgatttt tcctacaatt tcatgtgcag
     1501 ttctaaatgg aatattttta gttactaaat aatctgctag ttccgttgca tttgaaaaat
     1561 cttctttaac agtttgattg agtcgttctt tattaattgt catcgtttga atcatacctt
     1621 cgaaaatacg taaagaacct ttaattgtat ggacagcatc gaataaacct tctttatctt
     1681 cctgcatatc tttgttatat gctagaggta atccttttaa agtcataagc atgctcatta
     1741 aatgaccagt cgttcgacca actttacctc taattaattc tgccatatca ggatttttct
     1801 tttgtggcat aatagatgag ccagttgaaa atgcatctga taatgtaatg aatttagctt
     1861 cgtctgtgga ccagaaaata atttcctctg caaagcgtga taagtgaacc atcgttaaag
     1921 aaatattatg caatgtttca ataatatagt ctctgtcact aacagcatct aggctattct
     1981 catagagact gccaaagttc aacaatgctg ttgtctcgtg tctatcgata gggtatgtgg
     2041 taccacttaa ggctgctgca cctaaaggat taatatcgat tcgttttaaa ctatcttcaa
     2101 atcgttgttg gtctcgttgt aacatccaaa aataagtcat aatatgatgt gcaaatgaaa
     2161 ttggctgtgc acgctgtaaa tgagtataac taggcataat tgtatcaaca ttattggaag
     2221 cgatgtctac aattacactt tgtaacgact taattaatgc gatgatatct tgcacttgtt
     2281 tcttagtgta caagtgcatg tctgttgcaa cttgatcgtt tctactgcgt ccagtatgca
     2341 acttaccacc agcatcaccg atacgtttaa ttaattcatg ttcaatattt aaatgaatat
     2401 cttctaatga tgcactaaat tgaatttgat cttgatgata atcatgttga atagatttta
     2461 gtccttgtat aatttgttcg ctgtcttgtt gactaataat gccttgattc gcaagcatag
     2521 ttgcatgtgc aatgctgcct tcgatatctt gatctatgag cgtttgatca aaagtaatgg
     2581 atgcgttaaa gtcgtcaacc cactcttcag gttgtacttc aaatctaccg ccccaagctt
     2641 tattgctcat tgctatagcc tccatgcaac atcgcattta cttgagtagg taaaccatag
     2701 atatcgataa agccaacagc agcgtcttga ttaaatgcat cttcttttgt ataagttgct
     2761 aatttttcat catataatgt gtaaggtgat tttctaccat tcacgatggc attacctttg
     2821 aataatttaa ttctgacatc accacttacg tattgctgag tactatcaat aaataatttc
     2881 aagctatcag ttaaaggtga gaaccaaagt ccattgtata gttgttcagc aaattgcttc
     2941 tcaatgattg gtttaaagtg tgcgacatct ttcgttaacg taatcgtttc taatgcttta
     3001 tgcgctttta aaataacttc tgcagcaggt gcctcataaa tttctcttga tttgatacct
     3061 acaagtctat tttctacatg gtcaattctt ccgataccat gcttaccagc taatgcattc
     3121 aacgttaaaa ttaaatcgtc taattcatat gttttgccat caatttgaac tgggatgcct
     3181 ttatcaaacg ttaaaatgat ttcatcagca gtatctggtg tttcttctaa agcatttgtt
     3241 agatcgaacg catcctctgg tggcgcagca taaggatctt ctaaaatacc acattcattc
     3301 gctctgcccc atagattttg atcgatagaa taaggtgaat catggttgat tgatacaggg
     3361 atattatgtt taattgcata atcgatttct tcttcacgac tccatgccca ctcacgtaca
     3421 ggtgcgaatg ctttcaatga tgggtttaat gctttaatgg caacttcgaa acgtacttgg
     3481 tcattccctt taccagtaca accatgtgca atacctactg aatttgtttt ctcagcaatc
     3541 tctactaatt ttttagcgat taatggtctt gataaagctg aaactaatgg atatgcattt
     3601 tcatacatta aatttccttt gattgcataa cttacatact catcactaaa ttcttttgtt
     3661 gcatcaataa tatgacattc aactgctccc atatctaaag cttttttata aacgatgtct
     3721 aaatctttac cttcaccaac atctaggcaa caagctacaa cgtcgtatcc tttgtcgata
     3781 agccattgaa cggccacact tgtatctagt cctcctgaat atgctaaaac aattttctct
     3841 ttcataaaat tcacctcatt gtagttatct gaatatttcg tatgaacaca tgatagcaaa
     3901 cttttaatga aaataaaacc attattttac atatttattc ataaaatgcg atagagatag
     3961 ttagagttgt aagtgtaaat gcttatttat caatgtttaa aagtatttta aaaaacatgc
     4021 aaattgtata aaaaatgttt gagtgtttga cgccagtttg aaatgtttgt tgaaaaatag
     4081 gtgtgaaacg tcacataaat atcaatttta ttgattaagt acaaacttgt gaattgaatc
     4141 gcaaacgtaa acttagtaaa attaagatat taaatgaaac aggaggcttt tatagatgac
     4201 tcatattcaa ttagatttta gtaaaacgtt agaatttttc ggtgaacacg aattaaaaca
     4261 acaacaagaa attgttaaat caattcacaa aacaattcat gaaggtactg gtgcaggtag
     4321 tgacttctta ggctgggttg atttaccagt tgattacgac aaagaagaat tttcaagaat
     4381 tgttgaagca tcaaaacgca ttaaagaaaa ttctgatgtt ttagtagtca tcggtattgg
     4441 tggttcttac ttaggtgcac gtgcagcaat cgaaatgtta acgtcatcat ttagaaacag
     4501 caatgaatac cctgaaattg tatttgttgg taatcactta tcatcaacat atacgaaaga
     4561 gttagttgat tatttagcag acaaagattt ctctgtaaac gttatttcta aatctggtac
     4621 aactacagaa ccagcagttg catttagatt gttcaaacaa ttagttgaag aaagatacgg
     4681 taaagaagaa gcacaaaaac gtatatttgc aacaacggat aaagaaaaag gtgctttaaa
     4741 acagttggct acaaacgaag gttatgaaac gtttatcgta cctgatgatg taggtggaag
     4801 atattctgtt ttaacagcag taggattatt accaattgca acagctggaa ttaacatcga
     4861 agctatgatg attggtgctg caaaagcacg tgaagaatta tcttcagata aattagaaga
     4921 aaacattgca taccaatatg cgacaattcg aaacatttta tatgcaaaag gttatacaac
     4981 agaaatgttg attaactatg aaccatctat gcaatacttt aatgaatggt ggaaacaatt
     5041 atttggtgaa tcagaaggta aagacttcaa aggtatctat ccttcaagtg ccaactacac
     5101 aactgattta cattctttag gtcaatatgt acaagaaggc cgtcgcttct tattcgaaac
     5161 agttgtaaaa gtaaatcatc ctaaatatga tattactatt gaaaaagata gtgatgatct
     5221 agacggatta aattatttag ctggtaaaac aatcgacgaa gttaacacaa aagcattcga
     5281 aggtacatta ttagcgcata ctgatggtgg tgttcctaac atggtagtga acattccaca
     5341 attagatgaa gaaactttcg gttacgtcgt atacttcttc gaacttgctt gtgcaatgag
     5401 tggataccaa ttaggcgtaa atccatttaa ccaacctggt gtagaagcat ataaacaaaa
     5461 catgttcgca ttattaggta aacctggttt tgaagacttg aaaaaagaat tagaagaacg
     5521 tttataaaat acattacttc aaagattagt gaagtttgaa aagatagaac tagacgttaa
     5581 ctatttaaag catattttcg aggttgtcat tacaaatgta aaaatgtaat gacaacctcg
     5641 tttttattta tatgcaagaa ctaggttact agctaatgtg acaagatgtt aagagaaaat
     5701 taaagataaa ataatatctg ccttacaata atattgttat actactagag actgatttat
     5761 tagcatgatt acatgttaat gtttctttac ttagtaatta actttataat gtaagaataa
     5821 ttatcttcaa ccaaagaaag ggattgatga tttgtcgttt catcaagtag aagaatggtt
     5881 tgagatattt cgacagtttg gttatttacc tggatttata ttgttatata ttagagctat
     5941 aattccagta tttcctttag cactctatat tttaattaac attcaagctt atggacctat
     6001 tttaggtata ttgattagtt ggcttggatt aatttctgga acatttacag tctatttgat
     6061 ctgtaaacga ttggtgaaca ctgagaggat gcagcgaatt aaacaacgta ctgctgttca
     6121 acgcttgatt agttttattg atcgccaagg attaatccca ttgtttattt tactttgttt
     6181 tccttttacg ccaaatacat taataaattt tgtagcgagt ctatctcata ttagacctaa
     6241 atattatttc attgttttgg catcatcaaa gttagtttca acaattattt taggttattt
     6301 aggtaaggaa attactacaa ttttaacgca tcctttaaga gggatattaa tgttagttgt
     6361 gttggttgta ttttggattg ttggaaaaaa gttagaacag cattttatgg gatcgaaaaa
     6421 ggagtgacat cgtgaaaaaa gttgtaaaat atttgatttc attgatactt gctattatca
     6481 ttgtactgtt cgtacaaact tttgtaatag ttggtcatgt cattccgaat aatgatatgt
     6541 caccaaccct taacaaaggg gatcgtgtta ttgtaaataa aattaaagtt acatttaatc
     6601 aattgaataa tggtgatatc attacatata ggcgtggtaa cgagatatat actagtcgaa
     6661 ttattgccaa acctggtcaa tcaatggcgt ttcgtcaggg acaattatac cgtgatgacc
     6721 gaccggttga cgcatcttat gccaagaaca gaaaaattaa agattttagt ttgcgcaatt
     6781 ttaaagaatt agatggtgat attattccgc caaacaattt tgttgtgcta aatgatcaag
     6841 ataataacaa gcacgattca agacaatttg gtttaatcga taaaaaggat attattggta
     6901 atgttagttt acgatactat cctttttcaa aatggactgt tcagttcaaa tcttaaaaag
     6961 aggtgtcaaa attgaaaaaa gaaatattgg aatggattat ttcaattgca gtcgcttttg
     7021 tcattttatt tatagtaggt aaatttattg ttacgccata tacaattaaa ggtgaatcaa
     7081 tggatccaac tttgaaagat ggcgagcgag tagctgtaaa cattgttgga tataaaacag
     7141 gtggtttgga aaaaggtaat gtagttgtct tccatgcaaa caaaaatgat gactatgtta
     7201 aacgtgtcat cggtgttcct ggtgataaag tagaatacaa aaatgataca ttatatgtca
     7261 atggtaaaaa acaagatgaa ccatatttaa actacaattt aaaacataaa caaggtgatt
     7321 acattactgg gactttccaa gttaaagatt taccgaatgc gaatcctaaa tcaaatgtca
     7381 ttccaaaagg taaatattta gtgcttggag ataatcgtga agtaagtaaa gatagccgtg
     7441 cgtttggcct cattgatgaa gaccaaattg ttggtaaagt ttcatttagg ttctggccat
     7501 ttagtgaatt taaacataat ttcaatcctg aaaatactaa aaattaatat gaaacaaata
     7561 caacatcgtt tgtcggtttt aatactgata aacgatgttt tattttgtta gtaccacaat
     7621 aaaagctaag ttcgaaatga acttataata aatcaatcac aatcactttg tgttaaaata
     7681 tgtgtcaaag gaagtgaggg tttgtcatga cattacatgc ttatttaggt agagcgggaa
     7741 caggtaagtc tacgaaaatg ttgaccgaaa taaaacaaaa aatgaaagca gatccgcttg
     7801 gagatccaat cattttaatt gcgccaactc aaagtacatt tcaattagaa caagcctttg
     7861 tcaatgatcc ggaattaaat ggtagtttaa gaacagaagt gttgcatttt gaacgattaa
     7921 gtcatcgtat tttccaagaa gttggtagtt atagcgaaca aaagttatct aaagctgcaa
     7981 cggaaatgat gatttataac attgttcaag aacaacaaaa gtatttaaaa ctttatcaat
     8041 cacaagcaaa atattatggg tttagtgaaa aattaacaga acaaattcaa gattttaaaa
     8101 aatatgcagt aacgcctgaa catttagaac actttattgc tgataaaaat atgcaaactc
     8161 gaactaaaaa taagttagag gatattgctt taatataccg tgagttcgaa caacgcattc
     8221 aaaacgagtt tattacgggt gaggattcat tacaatattt tattgattgt atgccgaaat
     8281 cagagtggct aaaacgtgct gatatatata ttgatggttt tcacaacttt tcaacgattg
     8341 agtatttaat aatcaaagga ttaattaaat atgcgaagag tgtcacaatt atattgacga
     8401 cagatggtaa ccacgatcaa tttagtttat ttagaaaacc atcggaagtg ttacgacata
     8461 ttgaagaaat agcaaatgaa ctcaatattt ctattgaacg tcaatatttc aaccaattat
     8521 atcgcttcaa taatcaagat ttaaagcatc ttgaacaaga atttgatgca cttcaaatca
     8581 atcgagtggc atgtcaaggt catatcaata ttttagaatc tgcgactatg agagaggaaa
     8641 taaatgaaat tgcgcgacgt atcatcgttg atattcgtga taagcaatta cgatatcaag
     8701 atattgctat tttatatcgt gatgaatctt atgcttattt atttgattcc atattaccgc
     8761 tttataatat tccttataat attgatacaa agcgttcgat gacacatcat ccggtcatgg
     8821 aaatgattcg ttcattgatt gaagttattc aatctaattg gcaagtgaat ccaatgctac
     8881 gcttattgaa gactgatgtg ttaacggcat catatctaaa aagtgcatac ttagttgatt
     8941 tacttgaaaa ttttgtactt gaacgtggta tatacggtaa acgttggtta gatgatgagc
     9001 tatttaatgt cgaacatttt agcaaaatgg ggcgtaaagc gcataaactg accgaagatg
     9061 aacgtaacac atttgaacaa gtcgttaagt taaagaaaga tgtcattgat aaaattttac
     9121 attttgaaaa gcaaatgtca caagcggaaa ctgtaaaaga ttttgcaact gctttttatg
     9181 aaagtatgga atatttcgaa ctgccaaatc aattgatgac agagcgagat gaacttgatt
     9241 taaatggtaa tcatgaaaag gcggaggaaa ttgatcaaat atggaatggc ttaattcaaa
     9301 tccttgatga cttagttcta gtatttggag atgaaccaat gtcgatggaa cgtttcttag
     9361 aagtatttga tattggttta gaacaattag aatttgttat gattccgcaa acattggacc
     9421 aagtaagtat tggtacgatg gatttggcta aagtcgataa taagcaacat gtttacttag
     9481 taggtatgaa tgatggaacg atgccacaac cagtaactgc gtcaagcttg attacagatg
     9541 aagaaaagaa atactttgaa cagcaggcta atgtcgagtt aagtccaaca tcagatattt
     9601 tacagatgga tgaagcattt gtttgttatg ttgctatgac tagagctaag ggagatgtta
     9661 cattttctta cagtctaatg ggatcaagtg gtgatgataa ggagatcagc ccatttttaa
     9721 atcaaattca atcattgttc aaccaattgg aaattactaa cattcctcaa taccatgaag
     9781 ttaacccatt gtcactaatg caacatgcta agcaaaccaa aattacatta tttgaagcat
     9841 tgcgtgcttg gttatatgat gaaattgtgg ctgatagttg gttagatgct tatcaagtaa
     9901 ttagagatag cgatcattta aatcaaggtt tagattattt aatgtcagca ttaacgtttg
     9961 acaatgaaac tgtaaaatta ggtgaaacgt tgtctaaaga tttatatggt aaggaaatca
    10021 atgccagtgt atcccgtttt gaaggttatc aacaatgccc atttaaacac tatgcgtcac
    10081 atggtctgaa actaaatgag cgaacgaagt atgaacttca aaactttgat ttaggtgata
    10141 ttttccattc tgttttaaaa tatatatctg aacgtattaa tggcgatttt aaacaattag
    10201 acctgaaaaa aataagacaa ttaacgaatg aagcattgga agaaatttta cctaaagttc
    10261 agtttaattt attaaattct tcagcttact atcgttattt atcaagacgc attggcgcta
    10321 ttgtagaaac aacactaagc gcattaaaat atcaaggcac gtattcaaag tttatgccaa
    10381 aacattttga gacaagtttt agaaggaaac caagaacaaa tgacgaatta attgcacaaa
    10441 cattaacgac aactcaaggt attccaatta atattagagg gcaaattgac cgtatcgata
    10501 cgtatacaaa gaatgataca agttttgtta atatcattga ctataaatcc tctgaaggta
    10561 gtgcgacact tgatttaacg aaagtatatt atggtatgca aatgcaaatg atgacataca
    10621 tggatatcgt tttacaaaat aaacaacgcc ttggattaac agatattgtg aaaccaggtg
    10681 gattattata cttccatgta catgaaccta gaattaaatt taaatcatgg tctgatattg
    10741 atgaagataa actagaacaa gatttaatta aaaagtttaa gttgagtggt ttagttaatg
    10801 cagaccaaac tgttattgat gcattggata ttcgtttaga acctaaattc acttcagata
    10861 ttgtaccagt tggtttgaat aaagatggct ctttgagtaa acgaggcagc caagtggcag
    10921 atgaagcaac gatttataaa ttcatccaac ataacaaaga gaattttata gaaacagctt
    10981 caaatattat ggatggacat actgaagttg caccattaaa gtacaaacaa aaattgccat
    11041 gtgctttttg tagttatcaa tcggtatgtc atgtagatgg catgattgat agtaagcgat
    11101 atcgaactgt agatgaaaca ataaatccaa ttgaagcaat tcaaaatatt aacattaatg
    11161 atgaatttgg gggtgagcaa tagatgacaa ttccagagaa accacaaggc gtgatttgga
    11221 ctgacgcgca atggcaaagt atttacgcaa ctggacaaga tgtacttgtt gcagccgcgg
    11281 caggttcagg taaaacagct gtactagttg agcgtattat ccaaaagatt ttacgtgatg
    11341 gcattgatgt cgatcgactt ttagtcgtaa cgtttacaaa cttaagcgca cgtgaaatga
    11401 agcatcgtgt agaccaacgt attcaagagg catcgattgc tgatcctgca aatgcacact
    11461 tgaaaaacca acgcatcaaa attcatcaag cacaaatatc tacactccat agtttttgct
    11521 tgaaattaat tcaacagcat tatgatgtat taaatattga cccgaacttt agaacaagca
    11581 gtgaagctga aaatatttta ttattagaac aaacgataga tgaggtcata gaacaacatt
    11641 acgatatcct tgatcctgct tttattgaat taacagagca attgtcttca gatagaagtg
    11701 atgatcagtt tcgaatgatt attaaacaat tgtatttctt tagcgttgca aatccaaatc
    11761 ctaaaaattg gttggatcaa ttggtgacac catacgaaga agaagcacaa caagcgcaac
    11821 ttattcaact actaacagac ttatctaaag tatttatcac agctgcttat gatgctttaa
    11881 ataaggcgta tgatttgttt agtatgatgg atagcgtcga taaacattta gctgttatag
    11941 aagatgaacg acgtttaatg gggcgtgttt tagaaggtgg ctttattgat ataccttatt
    12001 taactggtca cgaatttggc gcgcgtttgc ctaatgtaac agcgaaaatt aaagaagcaa
    12061 atgaaatgat ggtcgatgcc ttagaagatg ctaaacttca gtataaaaaa tataaatcat
    12121 taattgataa agtgaagagt gattactttt caagagaagc tgatgatttg aaagctgata
    12181 tgcaacaatt ggcgccacga gtaaagtacc ttgcgcgtat tgtgaaagat gttatgtcag
    12241 aattcaatcg aaaaaagcgt agcaaaaata ttttggattt ttctgattat gaacattttg
    12301 cattacaaat tttaactaat gaggatggtt cgccttcaga aattgccgaa tcataccgtc
    12361 aacacttcca agaaatattg gtcgatgagt atcaagatac gaaccgagtt caagagaaaa
    12421 tactatcttg catcaaaacg ggtgatgaac ataatggtaa tttatttatg gttggagatg
    12481 ttaagcaatc catttataaa tttagacaag ctgatccaag tttatttatt gaaaagtatc
    12541 aacgctttac tatagatgga gatggcactg gacgtcgaat tgatttgtcg caaaacttcc
    12601 gttctcgaaa agaagtactg tcaacgacta actatatatt caaacatatg atggatgaac
    12661 aagtcggtga agtaaaatat gatgaagcgg cacagttgta ttatggtgca ccatatgatg
    12721 aatcggacca tccagtaaac ttaaaagtcc ttgttgaagc ggatcaagaa catagtgatt
    12781 taactggtag tgaacaagaa gcgcatttta tagtagaaca agttaaagat atcttagaac
    12841 atcaaaaagt ttatgatatg aaaacaggaa gctatagaag tgcgacatac aaggatatcg
    12901 ttattctaga acgcagcttt ggacaagctc gcaatttaca acaagccttt aaaaatgaag
    12961 atattccatt ccatgtgaat agtcgtgaag gttactttga acaaacagaa gtccgcttag
    13021 tattatcatt tttaagagcg atagataatc cattacaaga tatttattta gttgggttaa
    13081 tgcgctccgt tatatatcag ttcaaagaag acgaattagc tcaaattaga atattgagtc
    13141 caaatgatga ctacttctat caatcgattg taaattacat taatgacgaa gcagcagatg
    13201 caattttagt tgataaatta aaaatgtttt tatcagatat tcaaagttac caacaatata
    13261 gtaaagatca tccggtgtat cagttaattg ataaatttta taatgatcat tatgttattc
    13321 aatactttag tggacttatt ggtggacgtg gacgacgtgc aaatctttat ggtttattta
    13381 ataaagctat cgagtttgag aattcaagtt ttagaggttt atatcaattt attcgtttta
    13441 tcgatgaatt gattgaaaga ggcaaagatt ttggtgagga aaatgtagtt ggtccaaacg
    13501 ataatgtcgt tagaatgatg acaattcata gtagtaaagg tctagagttt ccatttgtca
    13561 tttattctgg attgtcaaaa gattttaata aacgtgattt gaaacaacca gttattttaa
    13621 atcagcaatt tggtctcgga atggattatt ttgatgtgga taaagaaatg gcatttccat
    13681 ctttagcttc ggttgcatat agagctgttg ccgaaaaaga acttgtgtca gaagaaatgc
    13741 gattagtcta tgtagcatta acaagagcga aagaacaact ttatttaatt ggtagagtga
    13801 aaaatgataa atcattacta gaactagagc aattgtctat ttctggtgag cacattgctg
    13861 tcaatgaacg attaacttca ccaaatccgt tccatcttat ttatagtatt ttatctaaac
    13921 atcaatctgc gtcaattcca gatgatttaa aatttgaaaa agatatagca caaattgaag
    13981 atagtagtcg tccgaatgta aatatttcaa ttgtgtactt tgaagatgtg tctacagaaa
    14041 ccattttaga taatgatgaa tatcgttcgg ttaatcaatt agaaactatg caaaatggta
    14101 atgaagatgt taaagcacaa attaaacacc aacttgatta tcgatatcca tatgtaaatg
    14161 atactaaaaa gccctcaaaa caatctgttt ctgaattgaa aagacaatat gaaacagaag
    14221 aaagtggcac aagttacgaa cgagtaaggc aatatcgtat cggtttttca acgtatgaac
    14281 gacctaaatt tctaagtgaa caaggtaaac gaaaagcgaa tgaaattggt acgttaatgc
    14341 atacagtgat gcaacattta ccattcaaaa aagaacgcat atctgaagtt gagttacatc
    14401 agtatatcga tggattaatc gataaacata ttatcgaagc agatgcgaaa aaagatatcc
    14461 gtatggatga aataatgaca tttatcaata gtgagttata ttcgattatt gctgaagcag
    14521 agcaagttta tcgtgaatta ccgtttgtag ttaaccaagc attagttgac caattgccac
    14581 aaggagacga agacgtctca attattcaag gtatgattga cttaatcttt gttaaagatg
    14641 gtgtgcatta ttttgtagac tataaaaccg atgcatttaa tcgtcgccgt gggatgacag
    14701 atgaagaaat tggtacacaa ttaaaaaata aatataagat acagatgaaa tattatcaaa
    14761 atacgcttca aacgattctt aataaagaag ttaaaggtta tttatacttc ttcaaatttg
    14821 gtacattgca actatagtat tttgattttc aaaagaataa aaaataattt cgattaagtg
    14881 caaagtcctt gtagcagaat gaacacaact cattttcaaa attgtcttac ttatttattt
    14941 gttatttgat aacgaaaaaa gttataatgt gaattaagat aaagatgagg agttgagaat
    15001 gaatgaaatt cttatcattc aagtataatg acaaaacttc atatggcgtt aaagtaaaac
    15061 gcgaagatgc tgtatgggat ttaacacaag tatttgctga ctttgcagaa ggagatttcc
    15121 atcctaaaac attgttagct ggtttacaac aaaatcatac tttagatttt caagaacaag
    15181 tacgtaaagc agttgtagca gcagaagata gcggcaaagc tgaagactat aaaatttcat
    15241 ttaatgacat tgaattttta ccaccagtaa cacctccgaa taatgtgatt gcttttggta
    15301 gaaattacaa agatcatgcg aacgaattaa atcatgaagt ggaaaaatta tatgtattta
    15361 caaaagcagc gtcatcttta acaggagata atgcaacaat tccaaatcat aaagatatta
    15421 ctgatcaatt agattatgaa ggtgaattag gtattgttat tggtaagtct ggtgaaaaga
    15481 ttccaaaagc attagcttta gattatgttt acggctatac aattattaac gatatcactg
    15541 atcgcaaagc acaaagtgaa caagatcaag catttttatc aaaaagttta actggcggtt
    15601 gcccaatggg tccttatatc gttactaaag acgaactacc attacctgaa aatgtaaata
    15661 ttgttacaaa agttaacaat gaaattagac aagatggtaa cactggcgaa atgattctta
    15721 aaattgatga attaatagaa gaaatttcaa aatatgttgc actacatccg ggagatatta
    15781 ttgcaactgg tacaccagca ggcgttggtg caggtatgca accacctaaa tttttacaac
    15841 caggtgatga agttaaagtg actattgata atattggaac gctgacaact tatatcgcta
    15901 aataattatc atttaaaaag ctaaccagtc tttatataga ttggttagtt ttttcttgct
    15961 tttctaaaaa ggtgttaaag ataaattatt tataatgtta ccattttgag atgaaagtga
    16021 aatattgata ttaagaagta gttgattatt ttacagcaga ttcacaatat tctaataagg
    16081 aataaaatgt catgttcttc ctctcaaata tagaagtgtg gtagaatata tattcgtgta
    16141 taatcaaatc atgattaaat tacaagcaag tgggtattaa tcccaagaag ctttaattta
    16201 ataatataaa taattaaagt ggggggaatc agtatgttac atttacatat attaagttgg
    16261 gtattagcga ttattttatt tatcgctaca tacttaaaca tttcaaaaaa tcaaggcaga
    16321 tcaccatttt tcaaaccgtt gcacatgatt ttacgcttat ttatgctgtt gacgttaatt
    16381 tcaggatttt ggatattaat tcagtcattt atgaatggcg gggcaaatca tatgttgctt
    16441 acattgaaaa tgctgtgtgg tgttgcagta gttggattga tggaagtgtc gattgctaaa
    16501 agaaagagac atgaacaaag tcacacaatg ttttggataa caattgcatt aattatcatc
    16561 acaatggtat taggtgtcat tctaccgtta gggcctatat caaaattatt cggtattggc
    16621 taattgataa gacatatagt atatgacata aggcatggac aattattagt ccaagacctt
    16681 atgtcttttt tgattctaaa aatgttaaac ataaaaaatg gactggatag tagtaccagt
    16741 ccaacataat tctaacctag acaattaaga ttagattgaa tcgcatgatt catttattta
    16801 gctttgtaac caatcatatt gattaaatct ttagggtggc tatatggtgg tgcataagcc
    16861 acttcaaact cagttaactc atctacagtt agctggttca tcattgccat cgatagtaca
    16921 tcaatacgtt tatctgcacc ttcttttcct actgcagctg ctcttaaaat ctgacggttt
    16981 gaagtgtcat aatatactct taagtgtaaa ggggaatttc ctgggtaata attcgcgtgt
    17041 gcaccttgag tgacttctac cattttatag tcaaattgct ttagttcgtt tggtttaacg
    17101 ccgacactcg caaatgtata atcaaagaac ttcacaatat tgttgcctaa gaagcctttg
    17161 aattcaatag tgtcatttcc agcaatttgt tcggcaacaa tacttgctgc acggtgagcg
    17221 ccccaagcta aaggaacact agccggtaga tcgacatgtc gataatgtga tgttgcaata
    17281 tcgcctattg cataaatgtt tggaacattt gtttcaaatt tatcgtttac cggtatgaaa
    17341 ccttttcgat caagtttgat atttgaactt tcgataaatt ttgaattggg gtgagtaccg
    17401 acaccttcaa taatcatatc gtaatgttca acttttcctg atttaaatgt aatttcattt
    17461 ccattgatag cattaatttc ctcatttaaa cggtatggaa tctcccgctt atctaattca
    17521 tcaagtatag gttgattcat gtcggcatcc attaatttat ttatcttatc agatcgatga
    17581 attaaagtag ggtgtaaacc acgttcataa agattttcaa gaacttctaa tgaaacataa
    17641 cctgcaccta caaccaatac tttatcaact tgatttgctt tgatgaattg atcgatagca
    17701 tcagtgtctt ctaaatttct aagtgtaaat gtaatatcac tttcaaagcc aaggctattt
    17761 gcacttgcac cagggcttaa aatgagttta tcgtaagatt cttcaaattg ttcgtttgtc
    17821 tttctattta atacagatac agtttgtctt tcatcattga ttgcaataac ttcatgataa
    17881 gtttttactg taatttgctt tctatcataa aatttttcag gtgtatacgc taaagcatat
    17941 cttctatctt caacaacttc gccaatgaca taaggcaatg cacaattagc aaagctcata
    18001 tcacgatctt tttcaaaaat aataatgtca ctttctttat ctaaacgtcg aatttggctg
    18061 gcacatgttg caccgccagc gactgctccg actacgacta ttttgggcat cgtaacttac
    18121 ctcctattgt tttaaaatat tataaaatga ttagttataa attaacagta atatctaata
    18181 tttaaattaa agaaatcatt caaatatcga ccaatgccat cttcattatt gttgaatgta
    18241 atattgttcg ctacatcttt aagttcttgc aaaccatttt ccatagcaac accgtgacgc
    18301 gcgtactcaa tcatttcaat atcattatct tcatcaccga atgcaataat attatttcgg
    18361 tcaatattta aaaattgtct aacttgctca atgcctcttg ctttattaat accaagtttt
    18421 acaatttcaa tgacagggaa tggtgcgccc cagcgtcgat gctcaatatg atcggcataa
    18481 aaatgagtaa gcatattttt gatttcaggt attttacttt cttcggcttc aattaaaatt
    18541 gaggtagggg attctttcaa gtggacaagt aaattaccag tttgaattct tggattaccc
    18601 attgaaaaac cttcaaataa tcttggatca tgattgttaa tgaaaacata atctttcact
    18661 tctgctataa tattcgatac ttgatattgt tgtaatcctt gaataatgtt ttgtgcgatg
    18721 cctaaatcta aaatttcatg gcaagttttg aagtttttat ctttagggtg atgtacgtaa
    18781 gcgccattaa aattaacaat tggtgtcgtt aaatttaatt catgataata catttgactt
    18841 gcacgataag gtctgccagt cgcaatcata atttggtgtc cacgttgttg taattcattt
    18901 aatacttgtt tagtatatga tgaaatttct ttgttatcgt ttaataatgt tccgtctaag
    18961 tctagacata ttaaatgtgg ttgcatatct ctctcctcat aagttgctta gttcatatag
    19021 atacatcata gcattttatt ggcgttttgt cggatatttg atatattatg aataatatga
    19081 cattaaagag gtgatatgga tggaagaggc attgaaagat agtatcttag gtgcattaga
    19141 aatggtaatt gaccctgaat taggaattga tatcgttaat ttgggtttag tatacaaagt
    19201 gaatgttgat gatgaaggcg tatgtacagt tgatatgact ttaacatcaa tgggatgtcc
    19261 aatgggacct caaattattg atcaagttaa aacagtatta gcagagattc ctgaaattca
    19321 ggatactgaa gtgaatatcg tatggagtcc accttggaca aaagatatga tgtcacgtta
    19381 cgctaagatt gcacttggtg tgagctaaca taaacttgca ttgcctgtag aatttctttt
    19441 cgaaattctc tgtgtttggg cccgcagtca atataacatt gtagagtata attcattgtg
    19501 aataagctcc cttcaaagta gacattgaaa aatgaaaatt ttgaagggag ccttatttat
    19561 gttctagttt ctgattttat tttgaaaatt tatatctaac ttgtttcaaa aaatatgaat
    19621 taagacagtg acttgcactt ccaagagtgt acaattcatt ttctagttga atattttctt
    19681 gaaaattttt ataataaatt taatttatat tacagttata ttacaataca taatacttca
    19741 tagtttttaa gttgccgagt tgcattacaa attggatttc gaagtaagaa tcatcattgg
    19801 taattttttt aatttaacag tattttagtc aatatgattt tgatgatgtc gtaattcaat
    19861 aatatacata tgcatttcaa tattctttta aacagcataa ctaaatttaa ttaagcatct
    19921 aagaagaaca gttttaaaag ggaagaatgc aaaaatgaac aaaacaaagg gttttacaaa
    19981 gtataagaaa atgagatata ttccagggct cgatggtttg agggcaatcg ctgttctagg
    20041 aattattatt taccacttaa ataagcaatg gttgacaggt ggctttttag gtgtggatac
    20101 attttttgtg atctctggtt atttaattac aagcttatta ctcaaagagt atgatgacac
    20161 aggtatcatt aaattgaaaa gcttttggat acgtcgttta aaacgtttat taccagcagt
    20221 catagtttta ttaatggttg tagggacagc aaccttatta ttaaaatcag ataatatcat
    20281 tagggttaaa catgatatta ttgctgcgat attttatgta tcaaactggt ggtatatagc
    20341 aaaagatgtt aattattttg agcaattttc atttatgcca ttaaagcatt tatggtcttt
    20401 agcaattgaa gaacagtttt acatattttt cccagttatt ttggttacat tattgttaac
    20461 aattaaaaag cgatacaaaa taggatttat tttttgggga gtatcaataa tttctttagg
    20521 gttaatgatg tttatctata gtattaatgg ggatcattca cgagtgtatt ttggtacaga
    20581 tactagatta cagacattgt tactgggtgt tattttagct tttttatggc caccgtttaa
    20641 attgaaaaat gatccaccta aagttgtaaa atatgttatt gatagcatag gtagtttatc
    20701 atttatagta cttatattat tatttttcat tattaatgat gagacgaatt ggatatatga
    20761 tggtggtttc tatttaatat ccatattaac gttatttatt attgctagtg tcgttcatcc
    20821 atctacatgg atagcgaaga tattttcaaa tccagtgtta gtatttatcg ggaaaaggtc
    20881 ttatagttta tatttatggc attttgcagt aattagtttc gtacatagtt actatgtaga
    20941 cggacagata cctgtatatg tgtactttat agatataagt ttaacaatta tatttgcaga
    21001 gctatcatat cgctttatag aaactccatt tagaaaagaa ggtattaaag ctttaaattg
    21061 gcgaccttct tatataccac aatttataag aatggcaatt gtagtaacct tgttaattcc
    21121 atttatgttg attttagtag gtgcattcaa taaatatggt aaagacatta ttggagaaaa
    21181 agcgaatagc tttgatacca caattgaaga taattattta atgcggatag caccaattga
    21241 taacatacac attgatggct tagtaagtga gaagaaaaag gaatcttccg acgtatataa
    21301 taatattaaa cctcttttaa tcggtgattc agtaatggtt gatatcggtg agtcatttaa
    21361 gtcatcagtt cctaagtcta gaattgatgg aaaagtaggg cgtcaattgt atcaaacctt
    21421 acctttagtt aaagcgaatt attcacaata taaaaaatca tctgatcaag tcgtattaga
    21481 attaggtaca aatggcgact ttactgtcaa acagctcgac gatttactta atcaatttgg
    21541 aaaagccaag atttatttag ttaatacacg tgttccaaga atttatgagg caaatgtaaa
    21601 tcgattatta gctgacgcgg cgaaacgaaa gtccaatgtc acattaattg attggtataa
    21661 gcgatcacaa ggacatagtg aatattttgc accagacggt gtacatttag agtacaaagg
    21721 agtcctagct ttaaaagatg aaatattaaa agcacttaaa aagaaataac atatgtttaa
    21781 taagtctagt taatgtgtaa cgtaacatta gctagatttt tttattcaaa aaaatattta
    21841 caaatattag gaaatttaag tgtaaaagag ttgataaatg attatattgg gactataata
    21901 taattaaggt caaagaaagt caaagtcaaa agtgcaactt gaccaactct acaaattaga
    21961 ggtgaaatta tatggatata aataaaatga catatgctgt tcaaagtgct ttacaacaag
    22021 cagttgaact gagtcagcaa cataaattac aaaatataga aattgaggca attttaagcg
    22081 ctgccttaaa tgaaagtgaa agcttatata aaagtatttt agaacgagca aatattgagg
    22141 tagatcaatt aaacaaagct tatgaagaca aactaaacac gtatgcatct gtagaaggtg
    22201 acaatataca atatggtcaa tatattagcc aacaagcaaa ccaattgata actaaggctg
    22261 aatcatacat gaaagaatat gaagatgaat atatttcaat ggagcatatt ttacgttcgg
    22321 caatggacat tgatcaaaca acaaaacatt atataaataa taaagtagaa gttatcaaag
    22381 aaattattaa aaaagtaaga gggggaaatc acgtgacatc acaaaatcca gaagttaatt
    22441 acgaagcatt agctaaatat ggccgcgact tagtagaaga agttagacaa ggtaaaatgg
    22501 atcctgttat aggaagagat gaagaaattc gaaatacgat tcgtatttta agtcgtaaaa
    22561 ctaaaaacaa ccctgtgctc attggtgaac caggtgttgg taaaactgca attgttgaag
    22621 gattagcgca acgtatagtt aagaaagatg tgccagaatc attattagat aaaactgttt
    22681 ttgagttaga tttaagcgca ttagtagcgg gcgctaaata tcgtggtgaa tttgaagaga
    22741 gattaaaagc agtcctaaaa gaagttaaag agtctgatgg tagaattata ttatttattg
    22801 atgaaatcca tatgcttgta ggtgctggta aaacagatgg tgccatggat gcaggcaaca
    22861 tgctaaaacc aatgttagca cgaggagagt tacattgtat tggtgcaaca actttaaatg
    22921 aatatcgaga atatattgaa aaagattcgg cattagagcg tcgtttccaa aaagtagcag
    22981 ttagtgagcc tgatgttgaa gatacaattt caattttacg tggtttaaaa gaacgatatg
    23041 aagtgtatca tggtgtgcgt attcaagata gagccttagt tgctgccgct gaattgtctg
    23101 atcgttacat cactgatcgt tttttaccag ataaagcgat tgatttagtt gaccaagcat
    23161 gtgcaacaat tcgtacggaa atgggatcaa atccaactga attggatcaa gttaatagac
    23221 gtgtcatgca attagaaatt gaagaaagcg cacttaaaaa tgaatctgac aatgcgagca
    23281 aacagagatt acaagaacta caagaagagc ttgccaatga aaaagagaaa caagcagcac
    23341 ttcaatctcg tgtagaatca gaaaaagaaa aaatagcaaa tttacaagaa aaacgtgcgc
    23401 aactagatga aagtagacaa gcgttggaag atgcacaaac aaataacaat ttagaaaaag
    23461 ctgctgaact acaatatgga acaattcctc aattggaaaa agaacttaga gaattagagg
    23521 ataatttcca agatgagcaa ggtgaagata cagatcgaat gattcgtgaa gttgtaacag
    23581 acgaagaaat tggcgatatt gtcagccaat ggacaggcat accagtttca aaattagttg
    23641 aaacagaacg tgaaaaatta cttcacttaa gtgacatctt gcataaacgt gttgtaggtc
    23701 aagataaagc ggttgacctg gtttcagatg cagtagttag agcaagagca ggtattaaag
    23761 atccaaacag acctattggt agtttcttat tcctaggtcc aactggagta ggtaaaactg
    23821 aattagctaa atcattagct gcatcattat ttgattctga aaaacatatg attcgtattg
    23881 atatgagtga atatatggaa aaacatgcag tatcaagatt gataggggca cctccaggat
    23941 atattggaca tgatgaaggg ggtcaattaa ctgaagcggt tcgtcgtaat ccatactcag
    24001 ttattttatt agatgaggtt gaaaaagcgc atactgacgt ctttaatgta ttattgcaaa
    24061 ttttagatga aggccgttta actgattcta aaggacgtag cgttgatttt aaaaatacta
    24121 ttattattat gacaagtaat attggatctc aagttttatt agaaaacgta aaagagactg
    24181 gtgaaattac agaatcaaca gaaaaagctg ttatgacaag tttaaatgca tatttcaaac
    24241 cagaaatttt gaatcgtatg gatgatatcg tattatttaa accattatct attgatgaca
    24301 tgagtatgat tgtagataaa atcttaacgc aattaaatat aagattatta gaacaacgaa
    24361 tctcaattga agtttctgat gatgctaaag cttggctagg tcaagaagct tatgaacctc
    24421 aatacggtgc aagaccatta aaacgttttg tacaacgcca aattgaaaca ccattagcac
    24481 gtatgatgat taaagaggga ttcccagaag gtacaacgat taaagttaat ttaaattcag
    24541 acaataactt aacgtttaat gttgaaaaaa ttcatgaata aaattgaacc aagaatgtga
    24601 tgattaatcg cattcttggt tttttagatt tgaaatttat tataattgtt cattggcaat
    24661 atacttttta aatgatttaa taaacaccaa tatttctggg ctttcttttt tgctgtatat
    24721 atatgtaaat gaaataggtg cttgtaaaat tttcgtgtta ataaccgaaa tattgtaatc
    24781 actatttgtt gttatataaa gaggcaggaa tgatatacct tgattcattt cgattaattt
    24841 aattgaagta tgcacatcat tgatagatag aaattgtgct ttttcataaa tatttaaaat
    24901 attattttta agtgatgacc aatattctgg atggttatca cttattattt tgtatttttc
    24961 aaataaagat gcctcagtta gaagatgatt attctcttta ttgggagcaa tcaatacaat
    25021 tttaccttcg catacttttt cagaatgaac ttctcttagt ttaggttgat ttctgctaat
    25081 cccgatgtca tacgtatgat tattaatatc tttttcaata ttttcatttt tgacatgaag
    25141 agaaacatcg ataaaaggat gctcgttaaa gaaagatttt aaaaatttgg gcataatgaa
    25201 tgtcgcgata tatgaagaca cgacaacatt taatttcgat tgaaacatcg tttttttaag
    25261 ttggatatgt ttgatgccac tttcatattg ttcaataaaa ctttgcgcaa ttggaagaaa
    25321 tgtatgacca tcttcagtca agataatttg atttttataa gtttcaaata gtttcacatt
    25381 gagatgctgt tctaaatttt taatttgctt atgtatagaa ggtatagtga gattaatttc
    25441 ttcactagct aatcgatagt ttaacgtctt cgctaaaatg acaaatgtat agtaccaatc
    25501 taaattcatg aacttacctc tttcaaaaaa gttaataatt cattagataa tatttaattt
    25561 ttattgcata aaaataatat taatattata tcagaggtta tcgagggggt gaagtaatat
    25621 gattcgaatt caagataata caataaggga tggtatgcaa caaagtaatg ttcgaaaaag
    25681 tctaattata aaaaaagaag tattgaaaca aattaacaag ttaaatataa attctgttga
    25741 agtaggcatg tgtacaacta tcgaggatga atttaatatt catcaattca gagacatttt
    25801 aagtcctgaa aaagaattag tagtattgac taggcttaat gaaaaagaaa taaaaaaaat
    25861 agtcaaattg aaaattcata atttagtggt aaaaatacta ttgccaatat ctgacttgca
    25921 tataaaagaa aagcttaatt tttcaaataa atattatatt cagaaaatca aagactgctt
    25981 ggatatatta aagaaagata aaaaaggagt agatatttgt tttgaagatg gaacaaggac
    26041 ttctagagaa aaattgaaag aatacatgga aattatttca aaatatcaag ttagaacagt
    26101 tacatttgcg gacactgtag gatgttcgac accattagaa tacggagata tttttaatta
    26161 ctttgtaaaa aaatattcta acataatttt ttctgctcat tgtcataacg atctagggtt
    26221 ggctactgca aatacattag ctgcaatttt aaatggtgca aagcaaatag aaactacatt
    26281 tttgagaatt ggtgagagag cgggtaatgc tcctattgag gaaataatta ctattttgac
    26341 aaaaaaacaa atagaaagta cggaattcac tttacccgac gtatataaaa ctagtattaa
    26401 tatttctaaa attctcgatt ttcaaatatc agaaaacaaa cctataattg gtgaaaatat
    26461 atttaaacat gaatcaggaa ttcatcaaga tggtactaaa aaaaatataa atatgtatca
    26521 atatttagtt cctagtgatt taggattgaa aaattcacaa gttgttcagt ttccaataag
    26581 taatatttct agtaagaaaa tcttgcacaa taaatttaaa tcaatagtta acactgaaga
    26641 aattgatgaa aatatttctt tctataaact tgtgaacaag ttttcacctg aagtagcacc
    26701 tgaagataca gtggatttac ttcagataat aaaaaggagg agtaaagatg gaaattttaa
    26761 atgattctgt aaaaagtttt gaaaaacttt atttacaatt acaagatggt tttccagtga
    26821 ttgttcctac tgatactaat tataatttat gtagtttacc taacaacgat ctttgtatcg
    26881 ataaaatatt tgaatataaa aagcgatcga aagataagcc attatcatta tttattgata
    26941 agccagagga ttggaaattg tatggagata atcaaaatac ggaaatagtt gataaactag
    27001 ttgaaatatt ttggcctgga ccattaaata ttattttaaa aaataaaaca agctataatt
    27061 atatgctcaa taattcagat agtatagcta taggatgtgt acaaaataaa acgatgagaa
    27121 gatttatttc atatattaat tcacctatag caattacttc agcaaatata tctggaactg
    27181 ccgatgatat tttaataact gaaaatgaag caattaagca catgggcgaa aatgtaagat
    27241 atatgctaag aagtcaaaat aaaactaact ataaaacatc tagtacgatt attaaagtga
    27301 cagataataa aattgaatta ttaagagaag gagatataaa gtttgaagaa ataaaagaaa
    27361 gactaggtac aggtattatt tatgaataag ttaatacttg ggatttattt ataccgaatt
    27421 ttttcacgag catactttta tttaccgttt ttattaattt actttttgat tcaaggttat
    27481 tccataatac aattagaaat attaatggcg tcttatggca ttgcagcatt tttattctct
    27541 ctatacaaag agaagtgttt taaaatttgt aacttaaaag attctaataa attagttgtt
    27601 agtgaaatat tcaaaatcat cggtttattg ttgttattat atcaaaatca atatttaatt
    27661 ttagtagtgg cacaaatatt attagggtta agttactcaa tgatggcggg tgttgatacc
    27721 gcaataatta aaagaaatat aacaaatgag aaatacgtac aaaataagtc aaatagctat
    27781 atgttcctat cattattaat ttcagggatt ataggtagtt atctttatgg aataaatatt
    27841 aaatggccta taataatgac tggtatattt tcaattctaa caattataat tattcgatgc
    27901 acattagttg aaaataggga attaaattta ataggagaaa caaagggaaa gataaagaaa
    27961 tttctaccag aagagaagtt ttggatattg cattattctt ttttaagagc gttaatatta
    28021 ggatttttta taggatttat tccaattaat atatataatg atttaaaact gaataattta
    28081 caatttattt cagtattaac ttgttacaca gttatgggtt ttgtatcttc acgttattta
    28141 actaaatact tgaattataa gtttgtgtca gaaatttgtt tagtaatatt tttaataata
    28201 tatacatatc aaagtttcat agcagttact atttctatga tatttttagg tatttcttca
    28261 gggttaactc gtccacaaac tataaataaa ctttctagca gtagtaactt aagagtgatg
    28321 cttaattatg cagaaacgtt atattttatt tttaatatcg catttttact tatgggtggt
    28381 tacttatata caataggaac tattcaatac ttaatattat ttatttcgtt attaattttt
    28441 atatatttaa taataatatt ttattttaca aggagagagc aacatgaaaa taaaaactga
    28501 atttaaaggg aacaatatac catatgaata cgcagcaggt gcagatgtga gtgattctat
    28561 taacgggaat ccaattaagt catttccatt tgaagtaatt gaattaccgg aagggactaa
    28621 atatcttgct tggtctttaa ttgactatga tgcaattcct gtatgtggct ttgcttggat
    28681 tcattggagt gtagctaatg taagtgttag tggcaattca atttctataa aagcagattt
    28741 atcaagaaca aagggcgact atgtacaagg taaaaatagc tttactagtg ggttgttggc
    28801 tgaagatttt tcagaaatag aaaatcacta tgtaggacct acaccacctg atcaagatca
    28861 tcaatatgaa ttaacagttt atgcgttaga tcattcttta aatttgaaga atgggttcta
    28921 cttgaatgaa tttttaaaag aagtaaatca acataaaatt gatcaaacaa gtattaacct
    28981 tataggaaga aaaatttaat actaaatatc tcatcaatat aaaattgttc aattaaaagt
    29041 acaaagaaac aaaggtttta atttatatat taggtacggc gttcgctata atgcaaagaa
    29101 gtaattaaat ttaagaaatg taaacttagt tattgtaatg tgaatttatt tgaaaaaata
    29161 gaaagtatta acaattatag cttttacatt aattaaaatt tatttttaaa aacaagtaaa
    29221 caatttacat acttataatt tttgaaactt ttcaatttgt gttatattga ttttgtaaga
    29281 tactttaact cacaaaggag agagattata tgaaattaaa atcatttata actgtaactt
    29341 tggcactggg catgatcgca acgactggcg ctactgtggc aggtaatgag gtatctgcag
    29401 cagaaaagga caaactaccg gcaactcaaa aagctaaaga aatgcaaaat gttccatata
    29461 caattgcagt agatggcatt atggctttca atcaatctta cttaaattta ccaaaagata
    29521 gccaattatc atatttagat ttaggaaata aagttaaagc tttgttatat gatgaacgcg
    29581 gtgtaacacc tgagaagatt cgaaatgcaa aatctgccgt ttacacgatt acttggaaag
    29641 atggtagtaa aaaagaagtg gatcttaaga aagatagcta cacagcaaac ttgtttgatt
    29701 caaattcaat taaacaaatt gatattaatg taaaaactaa ataaacactt aaaattatca
    29761 aattcaacat gaagcattta attttacagt gatgattata aaataattgc cttgatacaa
    29821 agattactcg taaatgacat ctttgtatta aggctttttc taaatttaaa agtgatgggt
    29881 tagaggtcat tgagctttaa aatattcaaa atacaaaaca ttaatggcca aaaataaaag
    29941 ccgcctttat ctgggcagct tcaataataa gaaagacata tttcatttta tactaaatag
    30001 ttattgtgat gaatctttcg gcggtttaat tactgcagca aaaattgctg tgaaaatcgt
    30061 gaacaatact gccatgataa ttggattcac tacatttaag ctgtctccac ctactaggct
    30121 attaagtaca aagttaacca tttgcattaa taataatgcc caaaagaatg ttacgaggtg
    30181 tttcatgtca ttctacctcc actttaatta tatatatttt attttaagtg aaagttagaa
    30241 atttgtatag taacatctca tatattttga ccatattata cagtttaaat aaatgatttt
    30301 atctgaatgg ctattctaaa ttaagcgcat taaaaccaat ttcatactga aatttgacga
    30361 taataaagca ttaaaatttt attaactagt caatattcct acctctgact tgagtttaaa
    30421 aagtaatcta tgttaaatta atacctggta ttaaaaattt tattaagaag gtgttcaact
    30481 atgaacgtgg gtattaaagg ttttggtgca tatgcgccag aaaagattat tgacaatgcc
    30541 tattttgagc aatttttaga tacatctgat gaatggattt ctaagatgac tggaattaaa
    30601 gaaagacatt gggcagatga tgatcaagat acttcagatt tagcatatga agcaagttta
    30661 aaagcaatcg ctgacgctgg tattcagccc gaagatatag atatgataat tgttgccaca
    30721 gcaactggag atatgccatt tccaactgtc gcaaatatgt tgcaagaacg tttagggacg
    30781 ggcaaagttg cctctatgga tcaacttgca gcatgttctg gatttatgta ttcaatgatt
    30841 acagctaaac aatatgttca atctggagat tatcataaca ttttagttgt cggtgcagat
    30901 aaattatcta aaataacaga tttaactgac cgttctactg cagttctatt tggagatggt
    30961 gcaggtgcgg ttatcatcgg tgaagtttca gatggcagag gtattataag ttatgaaatg
    31021 ggttctgatg gcacaggtgg taaacattta tatttagata aagatactgg taaactgaaa
    31081 atgaatggtc gagaagtatt taaatttgct gttagaatta tgggtgatgc atcaacacgt
    31141 gtagttgaaa aagcgaattt aacatcagat gatatagatt tatttattcc tcatcaagct
    31201 aatattagaa ttatggaatc agctagagaa cgcttaggta tttcaaaaga caaaatgagt
    31261 gtttctgtaa ataaatatgg aaatacttca gctgcgtcaa tacctttaag tatcgatcaa
    31321 gaattaaaaa atggtaaaat caaagatgat gatacaattg ttcttgtcgg attcggtggc
    31381 ggcctaactt ggggcgcaat gacaataaaa tggggaaaat aggaggataa cgaatgagtc
    31441 aaaataaaag agtagttatt acaggtatgg gagccctttc tccaatcggt aatgatgtca
    31501 aaacaacatg ggagaatgct ctaaaaggcg taaatggtat cgataaaatt acacgtatcg
    31561 atactgaacc ttatagcgtt cacttagcag gagaacttaa aaactttaat attgaagatc
    31621 atatcgacaa aaaagaagcg cgtcgtatgg atagatttac tcaatatgca attgtagcag
    31681 ctagagaggc tgttaaagat gcgcaattag atatcaatga aaatactgca gatcgaatcg
    31741 gtgtatggat tggttctggt atcggtggta tggaaacatt tgaaattgca cataaacaat
    31801 taatggataa aggcccaaga cgtgtgagtc catttttcgt accaatgtta attcctgata
    31861 tggcaactgg gcaagtatca attgacttag gtgcaaaagg accaaatggt gcaacagtta
    31921 cagcatgtgc aacaggtaca aattcaatcg gagaagcatt taaaattgtg caacgcggtg
    31981 atgcagatgc aatgattact ggtggtacag aagcaccaat tactcatatg gcaattgctg
    32041 gtttcagtgc aagtcgagcg ctttctacaa atgatgacat tgaaacagca tgtcgtccat
    32101 tccaagaagg tagagatggt tttgttatgg gtgaaggtgc tggtatttta gtaattgaat
    32161 ctttagaatc agcacaagct cgaggtgcca atatttatgc tgagatagtt ggctatggta
    32221 ctacaggtga tgcttatcat attacagcgc cagctccaga aggtgaaggt ggttctagag
    32281 caatgcaagc agctatggat gatgctggta ttgaacctaa agatgtacaa tacttaaatg
    32341 cccatggtac aagtactcct gttggtgact taaatgaagt taaagctatt aaaaatacat
    32401 ttggtgaagc agctaaacac ttaaaagtta actcaacaaa atcaatgact ggtcacttac
    32461 ttggtgcaac aggtggaatt gaagcaatct tctcagcgct ttcaattaaa gactctaaag
    32521 tcgcaccgac aattcatgcg gtaacaccag atccagaatg tgatttggat attgttccaa
    32581 atgaagcgca agaccttgat attacttatg caatgagtaa tagcttagga ttcggtggac
    32641 ataacgcagt attagtattc aagaaatttg aagcataact ataaaaatct tcagtaacgt
    32701 tgttttagtt actgaagatt ttttcagttt ctttatacta agatgagcga cagcacaatc
    32761 gtcataataa aatataaaat atttattaat aataagggga ttatccatgt agaaacaaag
    32821 taatgctctt tttttacctc ttgtgggttg aaaaatggat catcagagat agacttcttc
    32881 tttttcgaag atgacatttg atactttaat cttctaaaac cataacttgt cgcatcaaaa
    32941 atgccttctt gtacaagtaa aatcaaaaat atgctaataa aaataattaa tgaaacataa
    33001 aacaatatat ttaaatatgt aatgatagta tggctattaa aaagccatat aataaacgtt
    33061 aatattggcg ttattagtgc cattccaagc cattttttca acatttgatc actcccactt
    33121 atagaaaact cttacgcata gtttacatta aaatcagaca ttgaggaatg attttttaat
    33181 ttcttcagct ttattgaaat tctaaaatca atcattcttc attagtttaa agcaaaaaaa
    33241 tattgatata tagtaaatat tgtatatata atattagtta agatttcaga aaattttgaa
    33301 gggaatggaa atttagaaat cggaatttgt tagaggaggg gattagatgg ggaaatatat
    33361 tttcaaacga tttatttata tgcttatttc tttatttatt attattacaa ttacattttt
    33421 cttaatgaaa ttaatgccag gttcgccatt taacgatgct aaattaaatg ctgaacaaaa
    33481 agaaatttta aatgaaaaat atggattaaa tgatcctgta gctacgcagt atttacatta
    33541 tttaaaaaat gttgttacag gcgattttgg taattcattc cagtatcata atcaacctgt
    33601 gtgggatttg attaaaccga gactactacc ttcttttgaa atgggtctta cagcaatgtt
    33661 catcggtgtg atactgggac ttattttagg tgttgcagca gctactaaac aaaattcttg
    33721 ggttgactat acaactacag ttatttcagt tattgcagta tctgtaccat cttttgtact
    33781 tgctgtactt ttacaatatg tatttgcagt taaattaaga tggttcccag tagctggatg
    33841 ggaaggtttt tcgaccgcgg tattaccgtc acttgcatta tctgcagctg ttttagcaac
    33901 tgtcgccaga tacataagag cagagatgat agaggtatta agttcagact atattttatt
    33961 agcgagagct aaaggtaatt cgacaatgcg tgtacttttt ggacatgcac ttagaaatgc
    34021 tttaattcca attattacaa ttatcgttcc catgttagca agtattttaa caggcacttt
    34081 aacaattgaa aatatttttg gagttcctgg attaggggat caattcgtac gttcaattac
    34141 aacaaatgat ttctcagtaa tcatggcaat cacactatta tttagcacac tgtttatcgt
    34201 ttctattttt attgtagata ttttgtacgg tgtgatagat ccacgaattc gtgttcaagg
    34261 aggtaaaaaa taatggctga aaataaaaac aatttgtcga ttaacgacga tcattctaat
    34321 gcagctatga cgcatacctc tgacgctatc gcatcatctg attttattat tagagaatta
    34381 gatttgaatc aggaacctga aatgcaacga gaaagcaaaa acttttggca agatgcttgg
    34441 gctcagttaa aacgaaataa gttagctgtt gtcggtatga taggtttaat tatcattgta
    34501 atatttgctt ttatcggtcc agttataaat aaacatgatt atgctgaaca aaatgtagaa
    34561 catagaaatc ttccggcaaa aatacctgta ttagacaaag ttccattttt accttttgat
    34621 ggtaaagatg cagatggcaa ggatgcttat aaagcagcaa atgctaaaga aaattattgg
    34681 tttggtactg atcagttggg tcgagattta tggacaagaa catggaaagg tgctcaaatt
    34741 tcattgttta tcggtgttgt tgcagcgatg ttagatattt ttattggtgt tgtatatggt
    34801 gcgatttctg gattcttcgg tggacgtgtc gatacgatta tgcaacgtat acttgaagtc
    34861 atagcatcta ttccgaattt aattgtcgta attttatttg tattaatttt tgaaccatcc
    34921 atttggacaa ttatattggc tatgtctatc acaggctggt taggcatgag cagagttgta
    34981 cgtggagaat ttttaaaatt aaaaaatcaa gagtttgtca tggcttcgaa aacattgggg
    35041 gcttcaaaat tcaaattgat atttaagcat attttaccta atacattagg tgctatcgtg
    35101 gttacatcaa tgtttacagt acctagtgct attttcttcg aagcattttt aagtttcatt
    35161 ggtataggtg tacccgcacc tcaaacatcg ttagggtcat tagtaaatga tgggcgcgca
    35221 atgttattaa tttatccaca tgaattattt ataccagcaa tgattttaag tttattaatt
    35281 ctattctttt acttatttag tgatggatta cgtgatgcat ttgatccgaa aatgcgtaaa
    35341 taaaaagggg gcatagcata tgactgaaag aatattagaa gtaaatgatt tgcatgtttc
    35401 ctttgatatt acagcagggg aagtgcaggc agtgagaggc gtagattttt atttgaacaa
    35461 aggggaaaca ttggcaattg ttggtgaatc aggttcaggt aaatctgtaa caacaaaagc
    35521 aattacaaaa ttattccaag gggacacagg aagaattaaa aagggagaaa ttttattttt
    35581 aggggaagat ttagcaaaaa aacctgaaaa tgagttgatt aaattacgtg gcaaagatat
    35641 ttcaatgatc tttcaagatc caatgacatc tttaaaccca acgatgcaaa ttggtaaaca
    35701 agtcatggaa ccattaatta agcacaaaaa ttatagtaaa gcacaagcta aaaagcgcgc
    35761 attggaaata ctaaatcttg taggtttacc aaatgcagaa aaaagattta aagcatatcc
    35821 tcatcaattt tcaggtggac aaaggcaaag aattgttatt gcaaccgcat tagcttgtga
    35881 acctaaagtg ctcattgctg atgaaccaac gactgcatta gacgtaacga tgcaggcaca
    35941 aattttagat ttaatgaaag aactacaaca aaaaatcgat acagcaatta tttttataac
    36001 gcatgattta ggggttgttg cgaatattgc tgatagagtg gcagttatgt atggtggtca
    36061 aatggttgaa acaggagatg ttaacgaaat attttatgat ccaaagcatc catatacatg
    36121 gggattatta tcgtcaatgc ctgatttatc aacaacaaat gacacaccat tactagcgat
    36181 tcctggagcg ccacctgatt tattacaccc acctaaaggt gatgcatttg cgagacgtag
    36241 tcaatatgca ttagatattg attttaaagt agaaccaccg tggtttaaag tttcaccgac
    36301 acattttgtg aaatcttggt tattagacgc acgtgcacca aaagttgaac tacccgagct
    36361 ggtaaaacaa cgtatgaaac cgatgcctaa taattatgaa aaaccactca aggtagaaag
    36421 ggtgtcgttc aatgaaaaat gatgaagtgc tattatctat taaaaattta aagcaatatt
    36481 ttaacgcagg aaagaaaaac gaagtgagag cgattgaaaa tatttcgttt gatatataca
    36541 aaggggaaac attaggttta gtaggagaat cggggtgtgg taaatctaca actggtaaat
    36601 caattattaa acttaatgat attacaagtg gagaaatttt gtatgagggt attgatatac
    36661 aaaagattcg taaacgtaaa gatttgctta aatttaataa aaagatacag atgatttttc
    36721 aagacccata tgcgtcttta aatcctaggt taaaagtaat ggatatagta gctgaaggta
    36781 ttgatatcca tcatttagca actgataagc gtgaccgaaa aaaacgtgtc tatgatttac
    36841 ttgaaactgt tggattaagt aaagaacatg ccaatcgcta tcctcatgaa ttttcaggtg
    36901 gacaacgcca acgtattgga attgcccgtg cattagccgt tgaaccagaa ttcattatcg
    36961 cggacgaacc aatatcggca ttggatgttt caatccaagc tcaagtagtt aatttattat
    37021 taaaattaca acgtgaaaga gggattacgt tcctatttat agctcatgat ctatcaatgg
    37081 tgaagtatat ttcagatcgt attgcagtca tgcattttgg gaaaatagtt gaaattggac
    37141 cggcagaaga aatttatcaa aatccattac acgattatac taagtcttta ttatcagcca
    37201 ttccacaacc tgatcctgaa tcagaacgca gtcgcaaacg atttagttat attgatgatg
    37261 aagcaaataa tcatttaaga caattacatg aaattagacc gaatcacttt gtctttagta
    37321 ctgaagaaga agcggcacaa ctacgagaaa ataaattggt gacacaaaat taaggggaag
    37381 ggggaaatgc aatgacgaga aaatttagaa cacttatttt aattttgatt gctacaattg
    37441 cattaagtgg ttgtgctaat gacgatggta tttattcaga taaaggtcaa gtattcagaa
    37501 aaattttgtc atcagactta acatcccttg atacatcatt aataacggat gaaatatctt
    37561 ctgaagtgac tgcgcaaaca ttcgaaggtt tatacacatt aggaaaaggt gacaaaccgg
    37621 tgttaggtgt tgcgaaagct tttcctgaaa agagtaaaga tggtaaaact ttaaaggtta
    37681 aattaagaag cgatgctaaa tggagcaatg gtgacaaagt gactgcacaa gactttgttt
    37741 atgcttggag aaaaacagtt gaccctaaaa caggttctga atttgcatac attatggggg
    37801 acattaaaaa tgcgagtgat attagtactg gtaagaaacc tgtagagcaa ttaggtatca
    37861 aagcattaaa tgatgaaaca ttacaaattg aattagaaaa gccggttcca tatattaatc
    37921 aattattagc acttaatacc tttgcacctc aaaatgaaaa agttgccaaa aaatatggta
    37981 aaaattacgg tacggcagct gatagagcgg tatacaatgg tccatttaaa gttgatgatt
    38041 ggaaacaaga agataaaacc ttactatcta aaaatcagta ttattgggat aaaaagaatg
    38101 taaaattaga taaagtgaat tataaagtta ttaaagactt acaagccggt gcatcattgt
    38161 atgatactga atcagtagat gacgcagtta ttactgcaga tcaagtaaat aaatataaag
    38221 acaacaaagg attaaacttt gtgttaacga ctgggacatt ttttgtaaaa atgaatgaaa
    38281 aacaatatcc tgattttaaa aacaaaaatt taagattggc tatcgcacaa gcaatagata
    38341 aaaaaggata cgttgattca gtgaaaaaca atggctcaat tccttccgat acactaacag
    38401 ccaaaggaat tgcgaaagcg cctaatggca aagattatgc gagtaccatg aattcgcctt
    38461 taaaatataa tcctaaagaa gcaagagcgc actgggacaa agctaaaaaa gagttaggta
    38521 aaaatgaagt gacattttca atgaacacag aagatacacc agatgcaaaa atatctgctg
    38581 aatatatcaa atcgcaagtt gagaaaaatt taccaggagt tactttgaaa attaagcaat
    38641 taccgtttaa acaaagagta tcactagaac tgagtaacaa ttttgaagca tcacttagtg
    38701 gttggtctgc agattaccct gatcctatgg cttatttaga aacaatgacc acaggtagcg
    38761 cacaaaataa tacagactgg ggtaataaag aatatgatca attacttaaa gtagcaagaa
    38821 ccaaattggc acttcaaccg aacgaacgat atgaaaactt gaaaaaagca gaagaaatgt
    38881 tcctaggaga tgcaccggta gcaccaattt atcaaaaagg tgttgcacat ttaacaaatc
    38941 ctcaagtaaa aggattaatt taccataaat ttggtccaaa taactcactt aaacatgtat
    39001 atattgataa atcgatagat aaagaaacag gtaagaagaa aaaataatat gctttgtaaa
    39061 ttaggctgga gacatatctc cagtcttttt gtgttggata aaaactttgg gaataaaaat
    39121 ttaaaataag tcgtttttta aattactgaa attgattaaa tgcataaata actgaatatt
    39181 ctaaaaataa acttgtaata attttttcta tgagtaaact aaaaagaaaa aattagattg
    39241 aaagtaggag gcatatgtat ggggaagcta attaaatata tttcaatact tcttattgtc
    39301 gttttagtgt tgagtgcttg cggaaaaagc agtaataaag atgaaggagt aaaagatgct
    39361 actaaaacgg aaacctcaaa acataaaggt ggtaccttaa atgtagcatt aacagcaccg
    39421 ccaagtggtg tttattcttc gttattaaat agtacacatg cagattctgt agttgaggga
    39481 tattttaacg aaagcttatt agcaactgat aaaaaaatac gtcctaaggc atatattgct
    39541 tcatggaagg acatcgagcc ggctaagaaa atagaattta aaattaaaaa aggtattaaa
    39601 tggcatgatg gtaatgaatt gaaaattgat gattggattt attcaattga agtcttagct
    39661 aacaaggact acgaaggtgc ttattatcca agtgtagaaa atatccaagg tgcgaaagat
    39721 tatcatgaag gaaaaactga tcatattagc ggattgaaga aaatagatga ctacactatg
    39781 caggttacat ttgataaaaa acaagaaaat tacttaacag gatttattac tggaccttta
    39841 ttaagtaaaa aatatttatc agatgtacca attaaagatt tagcgaaatc agataaaatc
    39901 cgaaaatatc ctattggtat tggaccgtat aaagttaaga aaatcgttcc aggtgaggct
    39961 gttcaactcg ttaaatttga tgattattgg caaggtaagc ctgcactaga caaaatcaat
    40021 ttaaaagtta ttgatcaagc gcaaattatt aaggcaatgg aaaaaggcga tattgatgtt
    40081 gcgaatgatg ctaccggtgc aatggcaaaa gatgctaagt catctaatgc tggtctcaag
    40141 gtattatctg cgccaagctt agactacggt ttaataggat tcgtatctca tgattacgat
    40201 aaaaaagcta ataaaactgg taaagtgaga ccaaaatatg aagacaaaga attacgtaaa
    40261 gcaatgcttt atgcaattga tagagaaaaa tggatcaaag cgtttttcaa tggttacgct
    40321 agtgaaatca atagttttgt accatctatg cattggatag cagccaatcc taaggaccta
    40381 aatgattaca aatatgatcc tgaaaaagct aaaaaaatct tagataagtt aggttataaa
    40441 gatagagatg gtgacggatt tagagaagat cctaaaggta ataaatttga gattaacttt
    40501 aaacattatt caggttctaa tcctactttt gaaccaagaa ctgctgcgat aaaagatttc
    40561 tgggaaaaag ttggcttgaa aacaaatgtg aagttagtag aattcggtaa atataatgaa
    40621 gacttagcaa atgcatctaa agatatggaa gtgtacttca gatcatgggc aggaggtaca
    40681 gatccagatc catcagattt ataccacact gatagacctc aaaatgaaat gagaacagtt
    40741 ttaccaaaat cagatcaata tttagatgat gcattagact tcgaaaaagt aggcattgat
    40801 gaaaagaaac gtaaagatat ttatgttaaa tggcaaaaat atatgaatga tgagttacct
    40861 ggattaccaa tgttccaagg taaatcgata actattgtta acgataaagt acgaaactta
    40921 gacattgaaa ttggaactga tcaaagttta tataatttaa ctaaagaagc ttagtaatgt
    40981 aacctcgacg attttgtcga ggtttactta tatgggggga tgttatgaat aatgtattgt
    41041 tagaggttaa agatttagaa acatcattaa aaataaataa tgaatggtta gaaactgttg
    41101 aaaatatttc ttttgaatta tctaaaggag aagttttggg tatagtaggg gaatctggtt
    41161 gcggtaagtc catattaagt aagtcaatta ttaaattatt accagaaaag atatctaaac
    41221 taagtaatgg agaagttata tttgatggta aacgaatcga tacgctcaat gagaagcaat
    41281 tgccagatat tcgaggaaat gatattgcta tgatttttca agaacctatg actgctttaa
    41341 atcctgtatt taccataaaa aatcaacttg tggaatctat aaaatcacat aaaaaaattt
    41401 ctaaaaaaga agcaaataaa ttagcaaaag atttactaaa aaaagttgga attgctagac
    41461 aagatgaaat attaaatagc tatcctcatc aattatctgg tggtatgaga caaagagtaa
    41521 tgattgcaat ggccatttca tgttctccta aattattaat tgctgatgaa cctacaacag
    41581 cattggatgt cacgattcaa gcgcaaatat tagacttatt aaaagaattg caaaaggaaa
    41641 cgcaaatggc aattatgatg attacacatg atttgagtgt agttgctgag ttttgcgata
    41701 aagtcttagt tatgtatgca ggtcaaattg tagaatttgg aggcataaaa gaaatactac
    41761 acaatccgaa acatccttat acccaaaaat tattatcaac aattccaaaa cttaaagaag
    41821 agcagaaacg acttgaaacg atagaaggaa ttgtgccatc aatccaagca tttcacgtta
    41881 ataagtgcag atttgcaaat agatgtaaca aaaaactgga tatttgtaat aatcaatctc
    41941 ctaaaatgca tgtttgtgaa gatgtcattg tacgttgtca tttgtacaaa aatgaatata
    42001 aggagatata agaatggaaa atattttaga agtcaaccaa ataaaaaaat actacaaaat
    42061 taaaactgga ttattacaaa aaactcagta cgttaaagct gttgatgacg tatcgttttc
    42121 aataaaaaaa ggacaaactt ttggattagt aggagaatcg ggttgtggta agtcaacgtt
    42181 aggtaaagtg attatcaggc ttgaagatgc aacttcaggc tcaataattg ttaatggtga
    42241 agatataaca agattacaag gtaaaaaact cagaaaatca cgacaacaat atcagatgat
    42301 atttcaagat ccgtatgcat cattgaatcc gatgcaaatg gttggagata tcatttcaga
    42361 acctatttta aattataaaa aattgccaaa agaagaaata aaaaaagaag tactatattt
    42421 attaaaatgt gttggcctaa gtgaagatgc atattataaa tatgcacatg aattttcagg
    42481 tggacagaga caaagagtgg gaattgcaag agcattggct ttgcgtccga gtttaattgt
    42541 tgctgatgag cctgtaagtg cattagatgt atctgttcaa tctcaagtac tgaatttatt
    42601 aaaagattta caagaacaat ttaacttaag ctatttattt atcgcacatg atttaagtgt
    42661 agtaaaacat ataagtgatg tcattggagt tatgtattta ggtcatatag ttgaaatcgc
    42721 atctgataaa gaaatttatg aaaatcccaa acatccatat acaaaagcgt tgatttcatc
    42781 aataccacaa attgataaac ataataacaa tagaattata ttaaaaggag aattaccttc
    42841 gccaagtaat ccgccgcaag gttgtccttt tcatacaaga tgtccaattg ctcaagatat
    42901 gtgcaaaaaa agtatgccag aattaaagga cattggtaat gaacatcaag ttgcttgttt
    42961 ctacgtagat aaagtaggtg atttaaatga ttaaattaat acttaaaaga ttagggttaa
    43021 tgatcccgtt actaatttta atctcaatag ttgtattttc attagcaatc atacaaccag
    43081 gagatccatt ttcagatcta caaaacggta aaataaaaca agaagcaata aatgcacaaa
    43141 gggaaaaatt aggtctcaac gactcaatac cacatcaata cattagatgg gtcaatcatg
    43201 ttatacacgg tgatttaggt gagtcaatca aatataaaag gccggtaatt gatgttattg
    43261 aggaaagaat tccaaataca atattactcg gtgctatgtc attaattatt acttatatta
    43321 tctcatttgc tttaggaata acgtcaggta gatattctta cagtttgacg gattatactg
    43381 tgcaaatatt taattatttg atgttagcca ttccatcttt tattgcggga gtatttgcaa
    43441 tttttatttt ttcttttgaa ttacaatggt tcccgtttca aggttctgtt gatattaacc
    43501 ttaaagaagg tacttttgaa tattatatga gtaaaatata tcacacattt ttacctgcat
    43561 tcactttagg tttattatct actgctggtt atattcaata tttacgtaat gatattattg
    43621 aaaattctaa aaaagattat gtattgacgg caaggtcaaa aggattatct atgaataaaa
    43681 tttataataa acatatattg agaaattctc taatacctat tattacattt ttaggtgctg
    43741 atattgtaag tattttaggt ggagccgtga ttacggagac tatcttttca tataacggta
    43801 tcggtaaatt atttttagaa tcggtaatag gccaagacta tccattaatg atggcattaa
    43861 cgttattttt ctcattttta ggtttactgg gtaatttgat ttctgatatt acttatggat
    43921 ttatagatcc aagaattaga agtaactagg ggggattaga atgcaaaata agtcaaaatc
    43981 gccttttaaa attgcatttt ctaaatttat tcataataaa attgcaatgt tatcggttat
    44041 ttttttatta atcataacta ttgtatcaat tatagcgcca ttaatagctc cttttccagt
    44101 gaaccaacaa gatttattaa atataaaagg tgaaatgaca gcacaaaaca ttcttggtac
    44161 agactctggt ggtagggata actttagtcg tttgttatat gcaggtcgta tttcattatc
    44221 cattggaatt acatctacaa taggaatgct tttgattgga attacagttg gagtgatttc
    44281 tggttatttt ggaggtattg ttgatacatt attaatgaga ataaccgaat ttgttatgtt
    44341 atttccattt ttaatatttg caattgtatt aaatgctgca cttggagata aaattaaaaa
    44401 tccttatgga tctgccataa ttcttgttct agttattatc gtattaagtt ggggaggtat
    44461 tgcaagactt gttcgtggta aagtacttca agaaaaagaa aatgaatact ttttggcagc
    44521 aaaatcaatt ggtacaccca catataaaat tattttgaaa catcttttgc cgaatatatt
    44581 aagtgtagtt atcgtacaag caacattgtt atttgccggt atgattgtag tggaatcagg
    44641 attgagcttt ttaggattcg gaattagtaa agcaatacca tcttggggta atatgttgag
    44701 tgatgctcaa gaaggggatg ttataagtgg taaaccgtgg atatggatgc cacctgctat
    44761 aatgattaca ttaactatat taagtataaa ctttgtaggg gaagggatta aagatgcttt
    44821 taatcctaga ggtagacgtt aaataataaa agaggcacta gttaattcta gtacctcttt
    44881 attttatctc ttacgtccta aacccatcgc tttttccatt tttttgacag ttttaaatga
    44941 aactttgtgt gctttatctc taccttgatc taaaatatca tcaagtttat ctgagttata
    45001 gaaactttcg tatttttctt ggaattctac taaaaatgct ttaactattt cagcaaggtc
    45061 acctttaaat ttaccataac cttcgccctc atattttgcc tcaatatctt taattggcat
    45121 gtctgttaat ccagcgtata ttgaaattaa atttgttata cctggcttgt tgtcacgatc
    45181 aaatttaata ataccatctg aatcagttac tgcgctttta atttttttag ctgcaacatt
    45241 cggctcgtct aataatgaaa tgaagttttt agcattatca tcactcttac tcatttttct
    45301 tgttgggtct tgtaaactca tgacacgtcc accaacttta ggcatacgaa tttcaggttt
    45361 cacaagcaca tcattatagc gactattaaa tctatctaca aggttacgag tcaattcgat
    45421 atgctgcttt tggtcatctc caactggaac gatattagta ttgtaaagaa caatatcagc
    45481 tgccattaaa ggtggatatg ttaatagacc agcaggtata ccttcaactg ctttctgagc
    45541 tttatctttg tattgcgtca tacgctctaa ttctccaaca gaagcaatcg tagttaacat
    45601 ccatcctgct tgtacgtgtg cagggacttc agattgtatg aacaatgttg ctttgtctgg
    45661 atctatacca gaagctaaat aaatcgctgc taattgtctg gtctgtttac gtaattttaa
    45721 acgatcttgt ggcattgtaa ttgcatgttg atctacgata cagaaataac aatcatagtc
    45781 attttgcaca tcaacaaatt gttttagtgc gccaatataa tttccaatag taggaattcc
    45841 actaggttgg atgcctgaaa ataatgtctc catttaaatg cctactttct tagtaatatt
    45901 ttaataatta tcattataac agtatttctt aaaaatgtaa gatgtgttag aatatacgta
    45961 taacttacta ctaacaaagg gattaataat cttttttata agtgtagaaa cttatctgaa
    46021 aaattaggtt tttctcattt aattttaatg ttagatagtt ataataaata gtaacgatta
    46081 agaattttag aaaaaaatgt tttttcttga tctataacta aattttaagc aatgaatttt
    46141 attcattcaa ataggagagt gagatgtatg gtaacattat ttacttcacc aagttgcaca
    46201 tcttgccgta aagcgaaagc atggttacaa gaacatgaca ttccgtatac ggagcgtaat
    46261 attttttctg aacatttaac aattgatgaa attaagcaaa tattaaaaat gactgaagac
    46321 ggtactgatg aaatcatttc tacacgttct aaaacatacc aaaaattaaa tgttgatatt
    46381 gattcactac cattacaaga cttatattca atcattcaag ataatcctgg cttattacgt
    46441 cgtccaatta ttttagataa taaacgacta caagttggtt ataatgagga cgagattcga
    46501 cgtttcttac ctagaaaagt tcgtacgttc caattacaag aagcacaacg tatggttgac
    46561 taataaccat ttaactatgt gtcatacttt actaaaagat atgtcaagca acactatttc
    46621 tatagtgttg cttttttggt tttaatttta caataattgc ttagtatctt ttaaatcaaa
    46681 tgtgcaattg ttaatatagc tctatattag agtgttttgg atgaaacatt tgctatgata
    46741 aggtaaaatt aaattaagaa agtgctttaa tgctttaact gtataaatca tttaaatagt
    46801 tttgctacaa cgaatatgtt attataagtt accaaaatat tttagagaaa gaaatcaaac
    46861 aaataaattg tattttgttt gatgctttaa tacaatgtgg atactattca tcgactgtaa
    46921 ggagtgagat gatatgagaa tagaacgagt agatgataca actgtaaaat tgtttataac
    46981 atatagcgat atcgaggccc gtggatttag tcgtgaagat ttatggacaa atcgcaaacg
    47041 tggcgaagaa ttcttttggt caatgatgga tgaaattaac gaagaagaag attttgttgt
    47101 agaaggtcca ttatggattc aagtacatgc ctttgaaaaa ggtgtcgaag tcacaatttc
    47161 taaatctaaa aatgaagata tgatgaatat gtctgatgat gatgcaactg atcaatttga
    47221 tgaacaagtt caagaattgt tagctcaaac attagaaggt gaagatcaat tagaagaatt
    47281 attcgagcaa cgaacaaaag aaaaagaagc tcaaggttct aaacgtcaaa agtcttcagc
    47341 acgtaaaaat acaagaacaa tcattgtgaa atttaacgat ttagaagatg ttattaatta
    47401 tgcatatcat agcaatccaa taactacaga gtttgaagat ttgttatata tggttgatgg
    47461 tacttattat tatgctgtat attttgatag tcatgttgat caagaagtca ttaatgatag
    47521 ttacagtcaa ttgcttgaat ttgcttatcc aacagacaga acagaagttt atttaaatga
    47581 ctatgctaaa ataattatga gtcataacgt aacagctcaa gttcgacgtt attttccaga
    47641 gacaactgaa taacgatatt aattaagcaa cgcactatga aaggagactc tttggagtct
    47701 ctttttttat tgatttcaaa agctgaaaaa gtaatagtag ttaaacttac gtgtaatatt
    47761 ttgaggtgaa gatatgttag tagctttaaa tgaagaaaag gaacgcgtat tagcaactac
    47821 tgcattgaga aagacacaat atttttgtcc ggtgtgtggc aagcaagtta ttttaaagcg
    47881 tgggctcaaa gtaattagtc attttgcaca taaacattta gcggaacaaa aatgttttaa
    47941 taatgaaacg attaaacatt ataaaagtaa attgatttta gcacagatga tacagcaaca
    48001 aggatgtaaa gtagagatag agccatttta aaagaaataa aacaaattcc ggatattttg
    48061 attaataata aatatgttat tgagctacag tattcgccaa ttccttataa acagattctt
    48121 caacgaacgg aaggtttaaa gaaaatggga tataaagtaa gttggttatt aaatgatgtt
    48181 gattattgtc ataataaagt gaagttcaat cattttcaaa gtatgtttat taatccattc
    48241 actcgaaaac ttcatacgtt caatttagag aaaaaacaaa taatgatgtt tcaacaaata
    48301 caatatttag gcgggcacaa atatgtcgct gaaaaaagaa atgccaaaat tagtgagttg
    48361 tttaatgagg cgccttgtga ttatcatgct gtttataaat tatcaaagtt cgcaattaat
    48421 caatatatca aatattgtcg ctggcaaaat tctgttttag aacccacttt aagtgcaatg
    48481 tatcaattac agttaactga tcaagaagta gtgcacaatt atggttatat ttttccagag
    48541 caaatttata ttaaaaatca tcctattgag tggcaattac aagttgattt atggttaaag
    48601 aatggaaaaa gcaaattagt aaatgacaat cttaattatt ttaaactgaa aaaatttatt
    48661 gttgctctag aaagtaaaac agcaattata gaaaaactta ttaacaatta tttaaatatt
    48721 tgttcagata aaggtaatga cgtgcaaatt ttgttctaaa atgagaaaat tatatcaatt
    48781 atattttatt aggaggttta ctacatatga gtcaacaatt atcgagagaa gaacaggaac
    48841 gtaaatatcc tgaatataca tgggacttaa caacaatttt caaagatgat gaagcttttg
    48901 aggctgcatt taaagaagtt gaaaatgagt taggcaaaga agaacaattt aaaggacaca
    48961 ttggtgatag tgctgagaca ttatacaatg cgttagaatt agaagataca ttaggtacta
    49021 aattagaaaa agtatatgta tacgcgcacc taaaacaaga ccaagataca acgaacgaca
    49081 agtatactgg tatggagtca agagcacatc aattaattat taaatttagc tcggcatgga
    49141 gtttcttagt gccagagatt ttacaaattg atgaagataa aattcaatca tttgtaaatt
    49201 catatgataa attacaaaaa ttcgcatttg atttgaagtt gattaatgaa aaacgtcctc
    49261 atattttaga tgctgaaact gaaaagttat taacagaagc gcaggacgcg ttatcaacgc
    49321 catcaaatgt atacggtatg tttagcaacg ctgatttagt atttgaagat gcgatagata
    49381 aagatggaaa tgcacacccg ttaacacaag gtacatttat taagtattta gaaacagatg
    49441 atcgcaaact aagagaaagt gcttttagaa atgtatataa agcatatggt gctcataata
    49501 atacgcttgg cgctacgcta gcaggtgaag tgaagaaaaa tgtatttaat gctcgtacac
    49561 acaattacaa aactgcaaga gaaaaag
//
LOCUS       DANRDI010000002        45698 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.002, whole genome shotgun sequence.
ACCESSION   DANRDI010000002 DANRDI010000000
VERSION     DANRDI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 45698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.002"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            222..623
                     /locus_tag="PZ377_000042"
     CDS             222..623
                     /locus_tag="PZ377_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDM6356935.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(682..31947)
                     /gene="ebh"
                     /locus_tag="PZ377_000043"
     CDS             complement(682..31947)
                     /gene="ebh"
                     /locus_tag="PZ377_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HDM6356936.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(32345..33736)
                     /gene="norB"
                     /locus_tag="PZ377_000044"
     CDS             complement(32345..33736)
                     /gene="norB"
                     /locus_tag="PZ377_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDM6356937.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            complement(33893..35215)
                     /locus_tag="PZ377_000045"
     CDS             complement(33893..35215)
                     /locus_tag="PZ377_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6356938.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            complement(35246..36286)
                     /gene="tdcB"
                     /locus_tag="PZ377_000046"
     CDS             complement(35246..36286)
                     /gene="tdcB"
                     /locus_tag="PZ377_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDM6356939.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            complement(36381..37499)
                     /gene="ald"
                     /locus_tag="PZ377_000047"
     CDS             complement(36381..37499)
                     /gene="ald"
                     /locus_tag="PZ377_000047"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDM6356940.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            complement(37974..38852)
                     /locus_tag="PZ377_000048"
     CDS             complement(37974..38852)
                     /locus_tag="PZ377_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDM6356941.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(38872..42312)
                     /locus_tag="PZ377_000049"
     CDS             complement(38872..42312)
                     /locus_tag="PZ377_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDM6356942.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            42616..43956
                     /locus_tag="PZ377_000050"
     CDS             42616..43956
                     /locus_tag="PZ377_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDM6356943.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(44162..44494)
                     /locus_tag="PZ377_000051"
     CDS             complement(44162..44494)
                     /locus_tag="PZ377_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDM6356944.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(44578..>45698)
                     /locus_tag="PZ377_000052"
     CDS             complement(44578..>45698)
                     /locus_tag="PZ377_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDM6356945.1"
                     /translation="MGLEAVVAREIQELGYETNVENGRIFFEGDASAIVKANLWLRTA
                     DRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLHSVPDCQAIT
                     KKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNRRGYRLAQGE
                     APIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFNREFVSEQWN
                     IMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLSDIIKFSVKD
                     VNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLSTYILTSNKEF
                     EYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
BASE COUNT        11852 a         8169 c         7715 g        17962 t
ORIGIN      
        1 tcgtagagca tagaacattg atttatgtcc cagcctgaag atgatttttt taggaagtat
       61 attacagtac tacgtattag aaaatcatct ttgaatcagc gagtatttta aacacgtgtt
      121 acttatttaa gtgatgcgtg ttttttaatg gattacccaa catattttgt attacagtaa
      181 tagtaacttt aactaagtat tgaatagaaa gtaggtttat tatggcgaaa ataaattttg
      241 atgctgcgac gaaaggaaat ccaggcataa gtacatgtgc cattgtaatc aaagaagatg
      301 agcagcatta tacatataca catgagttag gcgaaatgga taaccacact gcagaatggg
      361 ctgcatgtat ttatgcacta gaacatgcac gtgaattaaa tgttcaaaac gcactattat
      421 atacagactc aaagctaatt gcagatagca ttgaagctgg ttatgtgaaa aacgcaaatt
      481 tcaaacctta ttttgatcaa atagaaatat ttgaaaaaga ttttgattta ttatttgtta
      541 aatggatacc gagagaacaa aacaaagaag cgaatcaaca cgctcaacag gcattgtata
      601 aattaattaa aaagaataaa taaaaggagc accgactgat gtgatgtcaa tcggtgctcc
      661 tctatttatc aaagaaacaa attatttttt atttttctta gctttctttt tcttcttttt
      721 agctgctacc tttttagaag tattttttga tgctgacttt ttgtctttgg attggttatc
      781 tttttgattt ttctttttag ccaataataa aggaacgtcc tcatctttag attcaatact
      841 agttgttgtt tcaacatctt cctctttatc tttgcgtcgt tttgcgaata agagtggtga
      901 atgttcgtta tctttcaaca ctttttcatc tgtgttttca tttgtaactt ccacatcttc
      961 ttcatcttct ttacgacgac gttttggtaa gaagaacggc gtatgtttaa ctttatcgag
     1021 tgactcgcca ttatttagcg aatctttttc ttcaacagta acatcttctt catcttcttt
     1081 tctgcgacgt ttcgcaaata aaagtggtaa atgttttgta tcgtctaaag tctcttttat
     1141 tgaatcttta ttattatctc ttatttctaa ttcttcctct tcatcttctt tacgacgacg
     1201 tttggcaatg aagaaccaga aactagctag taaaccagag atacccacca caccaatagc
     1261 gtttttaata acattaccga aattattact aaagtgacgc atatgaagtg gatcaatgtc
     1321 atcatcttca tcaagtttct ttttatgtcc aatagtcgaa ctgttaaatg gatgatttgc
     1381 agttccataa ccaattgtta aatggctatt agattcattt ccagtacttg acgattgttt
     1441 agcacgatca gatgttacaa tttgtactgc tgaaattcga gcaataccat tgttcaatgc
     1501 agcctcaact tgctgtaatg tatgcgcatt attaatatct ctaattgttt caagcacaat
     1561 ttcgttaatt tgattcattg ctgcttgttt ttcatcagct gtgccaattt cactatttcg
     1621 aatactattt attttttcat ttgaaaagtc tttaattcta ctaatagctt cttgcttgcg
     1681 tttagctagt tcttttgctg ttggattagc tgctttcatt tgagctttaa attgttccaa
     1741 ttgctcactt atttcatcga tgctttgcgc acgttgaatc gcttgaattg cttgttcttt
     1801 taattgattt aacttagcaa tagcttcttg cttctcttta tctgttagat cagtacgagc
     1861 tttgatttca tcaatcatat gttgaattgc atcttcaatc gatttaattg catttgattt
     1921 tgcttgttca atcgtaaatt gttctggatt aaattgttca atatgcgctt gttcttgttg
     1981 ttgaatttct tcaactgaat gaacatcagg tgcattgtta acatgatcaa ttgcttgttg
     2041 tttaagctta ttaacttctg caattgcagc ttctttttgt tccagtgtta atgtcacact
     2101 attattgatt tcattaatct tttgttgtgc cgcattttcg attgattcaa ttgcctgttg
     2161 tttgactact gacaattctt tttctacaac ttttacagga acattaacaa tcacttttga
     2221 tccatcaagc aatgtaactt caacttttgc tgttaagcta tcagaaatcg ttgcagttga
     2281 tggtgtatcg atgacttctg ctgaaagctg atcaattaag tttatgtgtg caagaatatc
     2341 ttgttctgta atgatgtcat cacgatgtac aatgagttca ccattaacta ggatttgctc
     2401 aggtgttgct tcaaaaattt catttacagc aggttgttca tcaacctccc atacatgtgt
     2461 atttctaatg tcatctaaac ctaagtttaa tcctcgattt aattgatcta tagtttgagc
     2521 attctcaacg ttttgtagtg ctcgtttttc agcttcgtca atttctttga gcgcttttgc
     2581 tttttgctca ggtgttaaat ctggatttga attgatttga tcacgcttcg cattagctaa
     2641 ggcttttatt gcatttttcg catcttcttt agctttcact aaatctttga tgtcttgcaa
     2701 tgcttgtgca agttgtgctt ttgcctgctc aatttcttct ttagtcagcg cattgttaat
     2761 gtcgttatga ccttgttgaa gtatttgatt aattcgatat ttaagtgctt gtttttcctt
     2821 atctgttaga tttggatttt gatcgatttc gtcaattaaa gcttgaactt gtttatcaac
     2881 atcttgtttc gcatcttctt tagctttcac taaatctttg atgtcttgta atgcttgcgc
     2941 aagttgtgct ttggcttgtt caatttcttc tttagtcatc gcattgttaa tgccgttatg
     3001 accttgttga agtatttgat taatacgatc tttaagtgct tgtttttcct tatctgttag
     3061 atttggattt cgatcaattt cgtcaattaa tgcttgaact tgcttatcaa cgtctttatt
     3121 ggcatcaatt tttgcttttg gtatttcatt ggcatgcact tgttcaatcg cgtggttgcc
     3181 tgctgtttga acttgagata cagcctgatt acttgttgct ttattaatgt tgttgatgat
     3241 gctgtttgcc aattcttctg ctttattttt cgcaataagc ttgtcttgat ccgtcgcatt
     3301 tgaagcttcg atttctttta gcttattagc taaagcttga ttaatagatt gaattgcctt
     3361 gtctttagca tcttgtagtc gttgatcacc attaagatta tggattgcat cattgactgc
     3421 ttggattgcg ccattgatat cattcacatt tgtgttatca ctatttagca atgtatttgc
     3481 tagacgtttg gcatcatcga agtttgtttt agcattatcg tcagcgtttt ggtaattgac
     3541 agtttgctct gcatttggaa tttcattgtc aactaaatgt ttcaatgttt ccattgcatc
     3601 atttaagtca atttgattat taacaatatc tgttacatct gatacagtat cggcattgtt
     3661 aattgcttta tgtgcaagat cttgttgctg ttgatttaat ccatttaacg aattaacaaa
     3721 cgcatttgct ttatctttgg catttgcaag gttttggtct ccatttaatg cattttgagc
     3781 atcgataata ttttgtttca attgctctgc ttcagctttt gcaattgcat taccttgcac
     3841 tttatctaac tcatgtgctg cattcgcaat tgcattatca taatttgctt tcaaattgtc
     3901 atctgcattg atgtaatttg tgctgctgat aacaccaagg tgctgttcgt caatatattg
     3961 ttgtactgca cccattgcat tatctaattt agttgctcta ttaattgctc gagatgcttt
     4021 atttagcgtt tcagcattat taatttgttg gattgctaat tgtctttgag cattgtttag
     4081 atgtgttaat tgatccaatc tttgtaatgc ttcagcttta cgattattaa gtctttcttc
     4141 accatttaat gctttcttag ctgcagtgac tgcatcattt aatttgataa cttgttttgc
     4201 agttaagttt tgaccagtcg atttatcaag tgcttgtttc gctttatcaa ccgtttcatc
     4261 atatacttgt ttgacttgtg tatctgcatt gacatagttc gtgctacctt tcgtgcgtcc
     4321 ttcattgtca gtgatttctt gtgacaattg atccattgca cgattaagtg ccttagcctc
     4381 atctacaatt tgttggattt gatttaaatc gtttgattga tcgatgcgac ctttaaatcc
     4441 atcttgttga gcattgttta atgcattcaa ttggtcgatg tcatgtttac catttgtttt
     4501 agcaagtgct acacgttcat caccatttaa tgcttgtttt gcatttaaaa tattttgaag
     4561 tgcttgatcc acttgttgct tattcgcatt ttgtttcaat acattttcag catcagcaat
     4621 agcttgttta taagcatttt gtttatctga atctgcttgt gtgtaatcta cagtttgctc
     4681 aacgttagca tgttcattaa ctgcttgttg taattgatcc atagattgat tcaattgcgt
     4741 tgcttgatct actaattcag caattggttg aagtttcgtc gcttgatcaa tgttttgttt
     4801 agcagttgca atttgatcag catttaaatg tgttaattgg tcaatagttt gtttcgcttg
     4861 tgttttagct tcagcgactc tctcattacc atttaactca ttttcttttt cttgaagctt
     4921 agttaatact tggtctacag cgtctttatt cgcatttgaa ccattagttg gatctgtaat
     4981 gctttctgca gcttgtaacg cttgatctac tgcttctttt ttatcagttg acgcttcagt
     5041 gtaatttggt tgagccttat ctgtattcac ttgatcaact ttatttttca atgtttccat
     5101 cgcgtgatca agttcagtag cagtttgaac atgttgtgca acctctgttc ttgtaggcgc
     5161 tgcatttata gcatcagtta atgcttgttg ttgtgcatga tttaagtttg gcaatgcatt
     5221 tacagttgtt actgcttgtt gttgatcacg agcaagtttt tgatcaccat gtagattatc
     5281 ttttgcagtt gttactgctt gtgtcaattg ttctacttgt gatttgtcga gtgttggatt
     5341 acctgtttgg ttaattaaat cttttgcatt ttgaactgct gcttggtaag catctttttg
     5401 cggtttatct tcattgataa acttgctacc agattctgtt tgttgttgat cttgaatact
     5461 attacgtaat gcttgcattg cttgatcaag cgcttttgct tcagctaatt tttgtgctac
     5521 ttcgccacga gttgctgcgt tgtttatttg gctttcaagt gcttgacgtt gcggattatt
     5581 caaaccattt aattgattta aatcagtaac cgcatgttgt ttatcgtctg caagtttttg
     5641 atcaccgtgt aggttatctt tagcttggtt aacagcttgt gtcaattgtt caacttgtgc
     5701 tttatcaagc gttggattgt tagtttgatt aattaaatct tttgcatttt gaactgctgc
     5761 ttggtaagca tctttttgtg gtttatcttc attgataaac ttgctacccg cttccgtttg
     5821 ctgttgatct tgaatgctat tacgtaaagc ttccattgct tggttaagtg cttgtgcttc
     5881 agttaatttt tgtgctactt cgccacgagt tgttgcatta ttaatttgat tttctagcgc
     5941 ttgttgttga gctggtgtta attgatctaa atgatttaga gaatttccag cagtttgctt
     6001 atcttgagct aatcgttcaa caccatctaa tgcattttta gatgatatta ctgcttgagt
     6061 cgcacttgat acattacctt tattgatagt tggattattt aatcctgcaa tgatagactc
     6121 agcattttga actgcttcat cataggattg ttttttattc ggttctgcat tgacatatgc
     6181 actgctcgca cgtgttgcat ctttgtcagc aatactttgt tgtaatgcat ccataagttg
     6241 atctaatgct tgtgcttcag tcaaatcttg cttaactgct gttctagttg tttcactatc
     6301 aattaacgta tcttccatat gtttttgtgc attgtttaga tgtgctaatt gactaaccgt
     6361 ttgcttagca tgatctttat cattttgtaa cttcacatca ccatgtaatg ctgctttcgt
     6421 tgtattcaca gttgtcgctg cttgattaat cgcattatta tctaatgttg cagtttgttc
     6481 attgataata ttatttgcgg cttgaacagc ttgatcatag ttttgttgct ctggttgatc
     6541 ttcgttgata tatttgctat ttgctttagt tgctgcgtta tcttgaatgc tctgtcttaa
     6601 accatgcatt gcttggttaa gcgtattcgc attttgttca atttgatgaa ctgcagttac
     6661 taaagttgca gctgtaactt ggtcttttaa tgctgttttt tgaggatcat ttaagcttgt
     6721 taaagcatca attgcttgcg tcgcatttgt tttagcaact tgtaaattat ggtctccatt
     6781 taacgcttga agtgttgttt gaactttagt aatagcttgt tctacttctg tttggttcgc
     6841 attttgacca gtttgtttgt taatcattgc tgctgcttca tttaccgctg tattataagc
     6901 agtttgttta tcagtatcag cattgatgaa gttaccacgt tgttcaacgg cttgatgatc
     6961 tgcaatcgca ttaattaagt tacccatcgc agtatttaaa gattctgctg tttgtttgtg
     7021 accattaact gcagcaatat cagatgattg cgtaatttgt gattgtaact gttgcttttg
     7081 cgcattattc aaatgatcta attgatcaag tctgttgttt gcttgttgtt tagctgctgc
     7141 aaggttttca tcaccatcta atgcaacttt cgtattattc acttgtgtcg ttgcaccatt
     7201 aacatcatct ggattcatcg taggcgatgt agtttgatta atgatattat tagcagctgt
     7261 tacagcttgg ttatagtcat tttgtctttg cgcagttgca tcatgataat cttcagacgc
     7321 taatgtcgtt tgattatccg caacactatt tcttaataat tccatcgcat gatctaacgt
     7381 ttgtgctttt tgattaatcg tagtgacatc tgtaacaagt ggcgcttgtg taatttgact
     7441 ttcaaatgat tgtttttgtg catcatttaa gtgtgttaag ttcgccaatt gttgttttgc
     7501 ttgttgtttt gcatttgcca aattgtcatt accatttaat gcttgtttcg ctgcgttcac
     7561 ttcattagtg atgctttcaa cttgtgcttg tacttcatta gtaccacttt gtttattcaa
     7621 taatgcttca gcttttgcta ctgcctgttt atacgcattt tgtttgtcag gatctgcatt
     7681 taagtaatta ccgtccgcta atgtttgagc tttatcatta atagcttgtg ataattgatc
     7741 cattgcttga tctaggctcg ttgctgtttg tttaacttgg ttcactgtag caatttcagg
     7801 cgattgatcg atttgacctt ttaatgcttg tttttgagca tcgtttaaat gcgtcatccc
     7861 acttacggca tctttagcat tttgcttttc agttgccaat ttagcttcac cgtttaagcc
     7921 atttttagca tcagtcactt gagttaatgc acgtgttatt tcatctgggt taagcgttgg
     7981 atttgtcgtt tgattgataa taccttccgc ttgagacact gcatttgtat atgctgtttg
     8041 cttatcggca tcagcatcat gatagttctc acttgctttg acagtatctt tgtttgcaat
     8101 accttgtttc aagttactca ttgctgtatt cacattttgt gcattttgtt tagtttgttc
     8161 aactgtagct aacgcttgtg cttgattgat ttggttacgt aatgcatcac gttgtggttg
     8221 atttaaatcg cgtaacgtat caagatttgc taaagcttct tgtttcgctt gtgctaattt
     8281 ttcatcaccg tttaatgcat ctttcgcttg attcatagtt gtcactgctt gtgttactgt
     8341 atcaggcgtc aatacaggtg ttggtatgcc atttgcgatt tgttgcgctt ggttagccgc
     8401 attgttatat gcttgttgtt tgtcttgatc cgcttgtgta aagtcaactg actgtggtac
     8461 ttgactgttc gcttgaattt gttgtttcaa tgtacccatt gcattattta acgcatcagc
     8521 attttgctta atagcattaa cacctgtaac aagttctgca tgcgacactt ggtcttttaa
     8581 tgctgttttt tgtggttgat ttaagtttgg taactgatca atggctgtat ttgcattgtc
     8641 tttagcaact tgtaagttat ggttaccgtt taaatcaccc ttagcttgat tcacttgttg
     8701 taacgcttga gccacttgtt gtggatccac gtttgcattt ggtgtaccac taattatttg
     8761 ttcagcatgt gctaccgcat tgttgtaagc attttgctta tcagtatcag cattgacaaa
     8821 gttaccattt gctttagttt ggtctttatc tgcaatacct tgtttcaatt gtgtcattgc
     8881 ttgatttaaa tcttgtgctg tttgcttaat cgtgtttaca tttgcaacag tttgcgcagc
     8941 atcgacttgt tgttttaatg catctttttg cgcttgattt aaatcatttg catttgtaat
     9001 cacttgtttc gctgcatctt ttgcacgttg tacattgtca ttaccattca atgcttgttt
     9061 cgcttcgttc acacgttgca tcgcttgttc cacttcagtt tgacttgcat tgctgccatt
     9121 tgctttagat aaaatacctt ctgcatgtga aaccgcatta gtataagcat ctttcttagc
     9181 ttgatctgca tcagtaaagt taatgctatt taatgtctca gttttatctt gtaatgcatt
     9241 gtttaagtta gtcatagcat tattcagatt ttgagcattt tgcttaacat tattaactgt
     9301 tgcaatatct ggtgcttgtt caacttgagt tgttagcgct tgtttttgag catcatttaa
     9361 atgatctagt gtaccaagtg ttgtcttagc atcttgtttg gcatcagcta atttttgatt
     9421 accgttaagt gcttgatttg tgtcattcac tttagttaat gcttgttgaa ttgtatttgg
     9481 atccattgtt ggatttgttg tttgttttaa caattcttct gcttttgtta ctgcattatc
     9541 atatgctgtt tgcttgtcag tatcagcatc gtgatagttc tcgctacctt taatttgtgc
     9601 tttattcgca ataccttgtt tcaattgtgt catcgcgtca ttaagcgttg tcgccgcttg
     9661 ttgaatagca ttgacgtttg gtacaagtgt tgcttgcgtg atttgtttgc tgtattcatc
     9721 acgttgcgct tggtttaagt ttggcaattg atcaatagca tgttgaacat tttgtttcgc
     9781 cgttgctaag tttgtattac catttaaatc atttttagct tgcgtaactt tatttaacgc
     9841 tgcagtaatt tcgctaggtg taacgacaac atcaggcgta gcactaatta atgcttcagc
     9901 tttcgctact gcttgattat atgcattttg cttatcagga tctgcattga caaagttacc
     9961 atcagctttt gtttgttctt tatctgcaat gccttgtttt aattgtgtca tagcattgtt
    10021 taactcttgt gctgtttgtt taacattgtt tacaccagct acagtagttg cattttgaac
    10081 ttgttgtttt aatgcgtctt tttgtgcttg gttaaggtca ttagagctat taattaatgc
    10141 tgttgcttcg tcttttgcat gttgaacgtt ggcattacca tttaatgctt gttttgcagc
    10201 attaacttgt ttgattgctt gttcaacttc agcttgtgtt gcattgccgc cattagcttt
    10261 tgaaatgata ttttcagcat ttgtaacagc agtgttatat gcttgttgtt ttgcttgatc
    10321 tgcatcagtg aagttaactg tcgctttcgt agcatctttg tcagctatcg cttgtttcaa
    10381 gttacccatc gctgtattta agttttgcgc attttgctta atttgatttg cttcatcaac
    10441 agtatgcgcg ccattaattt gatcagtaac tgtttgtttt tgcgcattat ttaagttatc
    10501 taatgaacct aatgattgtg acgcttgttg tttcgctgcc tctaggtttt cattaccatt
    10561 taatgcattt ttagttgtgc ttacttgtcc agcagcttga ttgataacag tcggatctaa
    10621 agttggactt gtagtttgat caataatacc ttgtgcagtt gtgacagcat tattgtacgc
    10681 atccttttta ttctgacttg catcagtata attttggttt tgttttgttg tcgcattatc
    10741 tgcaatactt tgacgtaatt tgtccattgc tgcatcaaca ttgtttgctt tttgctcatt
    10801 accttgtgct tctgcaacag tagtcgattg ttgtaccaat tgttttaatg cctctttttg
    10861 tgcatttgtt aaatggctta aaccgtcaat tgctgtattt gcgtgttgtt tagctttttc
    10921 aaggttttga gtaccattaa gcgctgcttt tgtagcattc acttgattca tagctgcttc
    10981 aacttgatct ttaggcacgt tcgtacctgt agatttattt aaaatatttt cagcattatg
    11041 aaccgcttca ttgtatgcat ttttcttctc tggatctgca tctgcaaagt tttgactgcc
    11101 aagtgtagtg tctttatcat tcaagctatt tttcaagtta cccatcgctg tattcagttc
    11161 ctgagcggtt tgaatggctt gcgttacttc agctacagtg tgcgcttgat tgatgtttcc
    11221 agtaacagca cctttttgtg cattgtttaa gttatctaac gtagataatg tttggtttgc
    11281 attgttttta gcatctgtta cttttctatc accattcaac gcttgtttcg tagttgttac
    11341 agttgaaagt gctgcttcaa cttgtgattg gttcgcattt gtaccatttg cttgattaat
    11401 aatattttca gcagcagtta ccgcattgtt gtatgctgtt tgtttttctg gatctgcatc
    11461 tgtaaagtta acactttgtt tcacttgatc tttgtcttgg attccgttga ttaaacgttc
    11521 cattgcttga tctaattgtt cagctttagt tttcacttga tttactgtgc taatatttgg
    11581 tgcatttgtc acttgcgtat ttaaatcttg cttttgagca ttatttaaat gacttaattg
    11641 gttgatgtta gcagtcgttt cttgcttttt attcgctaag ttttgtgcac cattaagcgc
    11701 agttttgtta gtgctcactt gattagctgc ttgtgttact gcagatggat caagtgtcgg
    11761 ctgactcgtt gcattaatga tattttcagc atttgtaact gctgtattgt atgcattttg
    11821 tttatctcta tctgcatcaa catatggttg actcgctttt acatcatctt tattcgcaat
    11881 actatttcga agttgattca ttgcattatc aagatttgtc gcgttgtctt tcacactttg
    11941 aacgccagtt acttgtgtcg cgctatcaat ttgaccagta atactttgtt tttgtgcatc
    12001 agataaatgt gtcatttgac gtaatgcatc tttagcttgt tgcttcgcag ttgctaagtt
    12061 tgtatcaccg tttaaacctg tttcagcatt attaacacgt tgtgttgctg ctgtaacatc
    12121 tgctgcattt gtaatcacat taggcgaacc atttacaatg tttttcgcag cattatatgc
    12181 atcagtataa gcaccttgct tatcaggact cgcgttagtg taattaccac tagctacaat
    12241 tgtgtcatga tcagcaattg cttgttttaa ttgatccatc gcattattaa gtgatgtagc
    12301 actatttttc agatcagtta cacctgatac acgttgcgca gcgttcactt gttgttttaa
    12361 tgctgttttt tgtggtgtat tcaaatgtgt caagttatcg atagcttgta tagcttcagt
    12421 tttagcgcgt tgtaagttcg cgtcaccgtt caaagcattt tcagtagacg ttacactttg
    12481 aattgcttgt tcaacagctt gcttatcttt attcgcaccg tgatctttat ttaagtaaga
    12541 ttcagcttgt gtgatagcat tatcaaatgc tgttttctta gttggatctg cgtcgatgta
    12601 attttcgctc gctttagttt gatctttatt tgctatagct gattgtaatc gttgcattgc
    12661 accatctaag tcttgtgctt tcgttttaac accatttaca ccgttgactg ttgttgcgcc
    12721 ttcgatttgt gatgttaaat cttgtttttg tgcattattt aagtgtgtta atgtacctaa
    12781 gtttgctttc gcatctgatt ttgcttgtct tacatttgcg tcaccgttta aagcattctt
    12841 agcagtcgtt acttgattta atgcagcttc aacttgtgct ttcgtcatat tttgaccgtg
    12901 tgctttatct aaaatacctt gagcattcgt aacagcattt gtgtaagcat ctttcttacc
    12961 ttggtcagca tctgtaaagt tcacttgttg tttaacagtg ttttggtcat ttataccatt
    13021 ttgaagttga cccattgctg tatttaattc attagcagta ttttgtgcag cagttacgcc
    13081 tgctactgtc gttgcattgt caatttgact aattgctgca gttgattgcg cattattcaa
    13141 gttactatat gaagctaatg cacgtttcgc tgactctttt gcttgagcaa ctttattatc
    13201 accattcaag tcatttttag cttgattcaa ttggtttagt gctgcttgaa cttgtgtttc
    13261 attcgcgttg ctgccattag cattagtaat tgcttctgct tgtgtaaccg cagtatcata
    13321 cgcttgttgt tttggttgat ccgcatctgt gtaattgaca ctacgtttcg tattatcctt
    13381 ttcagcaagt gcatgtttca aattacccat cgctgtgttt aacgatgttg ctttattttt
    13441 agccgctgtg acagctgcaa gatttggtgc ttgatccact tcagcatttg ccgcagttcg
    13501 ttgtgcattg ttcaagtctg tcaaacgacc gatatctgat ttcgcagttt gttttgctgc
    13561 tgctaatttt tcatcaccgt tcaatgcaga cttcgcactg ttcacttggc tcgctttttg
    13621 gttaattgta tcaggattca tttcagggtt attcgttgca ctaataatac cttcagcatt
    13681 agttaccgca tcattgtatg cattttgtaa atctgcattc gcgtcttgat aatcttcgct
    13741 tgatttagtc gcatctttag aagcaatact ttgtcttaat tgattcattg cttgatctaa
    13801 agtaccggca ttagcttgaa taccttgaac acctgcaaca gtcgtaccac tttcgatttg
    13861 cgatgttaag tttgcttttt gagcatcagt taagtgtgac attgtcgcta cttgttgttt
    13921 agctgtgttc ttcgcttcat ttaatcgctc gtcaccattt aatgctgttt tagcagtatt
    13981 cacacgttgt aatgcttgtt caacagctgc tttattgtca tttgaaccag ctgttttatc
    14041 taataacgtt ttagctgctg ttacagcgtc attgtatgca gtttgttttt ctctatctgc
    14101 atcagtatat ttctgagctg cttttgtagc tgcttcatca ttaataccat tttgtaagtt
    14161 gctcattgct gtatttaatt cagatgccgt tgtagacact tgatttacac ctgcaactgt
    14221 tgtcgcacct tcaatttgtg atttcaatgc ttctttttgt gcattattaa ttgatgttag
    14281 gttattcaat gcatttttcg cagttgtctt agcgttcgca acattttgat taccgttcaa
    14341 ttcgttttta gcacgttgta cattttctaa cgcagtttcg acaccgtctt ttgaagtatt
    14401 tggaccttgt gctttattta aaatttgttc agcttgcgtc actgcatttg tatatgcatt
    14461 acgtttcgct tcgtcggcat cagtgaagtt aacaccttgc ttaatcgtat tctgatcttg
    14521 aataccattt ttcaagttcg tcatagctgt atttaatgca tccgcattat tttgtgcttg
    14581 tgttacttca ttaacatgcg ttgcaccttc gatttgacgt ttcactgcat ctttttgagc
    14641 gtcagttaag tcacttaagc cgttcaaatg ttgcttcgca tttgtttgcg cagttcttaa
    14701 gttttcttga ccgtttaacg cttgttgttt agctgtcact tggtctttcg cttgattaat
    14761 ttcttgcgca ttcattgatg gactagttgt ttgaccaatg attgcttttg ctgcagtgac
    14821 tgcactgttg tagtcagttt gtttgttttg acttgcatct gtgtagtttt gacctgcttt
    14881 aatcgttgct tcattcgcaa tgctatctcg tagacctttc attgcagtgt ttaatgtttg
    14941 tgcattatca cgaacagttt gaacatttgg caacgtcgtc gcttgaccca cttgttcttt
    15001 aagttttgct ttttgcggac catttaaaga agttaaacca tcaattgcag tgttagcatt
    15061 ttgtttcgct acttctaaat tatgattacc gtttaactgc gtcttagcat tcgttacttg
    15121 cgttgctgca tttgtaacat ctgctggtgt taacgttggt gttggtgtac cactaacgat
    15181 attttcggca gctgtaactt tttcatcata tgcttgacgt ttattcgcat cagcattgac
    15241 atagttacca ctagcttttg tctcagcttt atcagcaatg gcacgcttta aagctgtcat
    15301 cgcagtattt aattcagtcg cagtatgttc aacaccattt gctgcagata cacgtcctgc
    15361 acttgttact tgtgctttta atgcttcttt ttgttttgta tttaagtccg aagcatttgt
    15421 aatcgctgtg ttagcagcct gtttcgcacg atctaagttt tgaataccgt ttaatgcagt
    15481 atttgcttgt gtaacagctt gcattgctct ttcaacatct gctttaggtg tattaccgcc
    15541 agctgtttta tttaaaatag ttgctgctgc atttactgct tgagaataag cagttcgttt
    15601 agcatcatca gcatcttgat aattttgact ttgtaacgtc gtgtctttat cacgaattga
    15661 tgtttctaat tgacccatag caccatctaa ttgttgcgct ttggctttaa ctgtattaac
    15721 accttcaaca tttgttgctt gtgtaatttc attgtctaac gctgtacgtt gtgcattatt
    15781 aatgtgtgtt aatgtaccta acgtttgttt cgcagctgct ttagcttcat ttaatttcgc
    15841 atcaccgttc aacgccgtct tcgtactgtt cacattttgc aatgcttgtt caactgctgc
    15901 tttgtctaca ttttgaccac tagcttttgt taaaattgct ttcgctgcat ttactgcttg
    15961 atcataagct gatttcttac ttggctctgc atctaggtat ttctgagttt gttttgtttg
    16021 tgtctcatca ttgataccat tttgtaaact atgcattgcg ttatttaatt ctgttgcttt
    16081 tgcagtttct tgatttacac cagctactgt tgttgcacca tcaatgctac gcgttaacgc
    16141 atctttttgt gcattgttaa ttgatgttaa gttattcaag ttgtttttcg cagttgtctt
    16201 agcttgagct aagtttcgcg caccatttaa tgcctgttct ttagttgtca cttgtgatgt
    16261 tacttgcgta atagtatttg gttccatcgt tgggttcgat gtttgattaa tgattgcttt
    16321 tgctgcagta actgcactgt cgtactcgtt acgattattt ggacttgcgt cagtgtagtt
    16381 ttgacctgct ttaattgttg cttcattcgc aatactatct cttaagcctt tcatcgctgt
    16441 attcaacgtt tgagaactat ttttaacagt ttgaacacca tctaatgtag ttgcactttg
    16501 aacttgttct tttaattttg ctttttgtgc attattcaat tgtgctaagc cgtcaattgt
    16561 attgttggca tgctctttcg ctacacgtaa gttgttatca ccatttaatg ctgttttagc
    16621 cgcattgact tgtgatgccg cactatttac gtcattgact gttaatgtcg cattcggtgt
    16681 accactaatg atattttcag cgttagtcac ttttgaatca tacgcttgac gtttttcttg
    16741 atcagcattg acataattac cgcttgcttt tgttctttct ttatcagcaa tagctgcttt
    16801 aagtgctgtc atcgcactgt tcaattcagt tgcagtgtgt tgaacgttat ttgcatcaga
    16861 tacacgttgt gcattgttaa cttgtgtttt taatgcatct ttttgttttt ggtttaagtc
    16921 atgtgcgttg ttaattgtgt tcgtcgcatt tgttttagct tgagctaagt tttgtgcacc
    16981 atttaacgct gcttttgttg tattgacttg atttgccatg ccattaatcg cattagcatc
    17041 catatttgga ttgttcgttg cattaataac accatttgca ttatttacag cagtattgta
    17101 attatttttg ttgctgtcag atgcatcaag ataattttga cttgttttcg tcgtattatc
    17161 attttggata cttgtacgta atgcacccat ggcagtattt aacgtattac ctttatcttt
    17221 aacaccattt acacctgcta cattttgcgc ttgttcaact tgatgcttca agttgtcttt
    17281 ttgtaattgt gttaagttag gtaaaccatc aatcgtattt gttgctgaag ttttcgcatt
    17341 tcttaagttg tcagcaccat ttaaagccgc tttcgctctt gtaactgcat ttaatgcatt
    17401 atcaacgtct gctttagatg tgttaccacc agtttgttta tttaaaatgc cttcagccgc
    17461 tgtgacagct tgcgtatatg catttcgttt tgattcatcc gcatcaagat aattttgatt
    17521 tcttaatgtc gcatctttat cattgattga accttgtaag ctattcattg cgccgtctaa
    17581 tgtattggca tttgttttta cagtattaac gccatcaaca gtcgtcgctt gattgatttg
    17641 gccttctaag tcagtacgtt gtgcattcgt aatatggttt aaagtgccta agttttgttt
    17701 agctgccgct ttcgcttctg ccagttttgc atcaccattc aatgcatctt tcgtacttgt
    17761 tacttgttgt aatgcacggt caactgctgc tttatctgaa tttgaacctg tttgtttatt
    17821 taaaattgct ttcgcagcag ctacagcttg atcataagca gttttcttac tttgttcagc
    17881 gtcacgatat ttttgagttt gttttgttgc attttcatca tcaataccat gttgtaattg
    17941 acccatagct gtattaagtt catttgcagt ttgttggata cttgttacat ttgcaacacg
    18001 ttgcgcactt gtaacttgtg cttttaacgc atctttttgc gctttattta agttagtagc
    18061 accatcgatg gcatttgttg ctgtttgttt cgcttgcgtt aaattatgtg taccatctaa
    18121 tgcatttttc gttgatgtca cttgtgtagc gatttggtta attgcattag catccatatt
    18181 tggattgctt gttgcattaa tgacaccatt agcactatca acagcattgt tatagtttgt
    18241 tttgttacgt tctgtagcat caagatagtt ttggccattt ttcgtagctg tgttatcttg
    18301 tatgctattt cttaacgtac ccatagcacc atttaatgtg ttggcacttg atttaacagt
    18361 atctacacct gcaacatttt gtgcttgttg cacttgttgt ttcaatttat ctttttgagc
    18421 agttgttaaa tgttgaagtc cgtttatagt agtgtttgca tttgatttag cttgctctaa
    18481 attatggtta ccatttaggg cacctttcgc attagcaact tgtgacaacg cgttttcaac
    18541 ggcagttttg tctaaatttg atccactatt tttatttaaa atattttctg cgttcgtaac
    18601 ggcttgagta taagcagttt tcttatctga atcagcatca tgatagtttt cacttgctag
    18661 tgtatttgtt ttatcattaa taccattttg caagttagcc atcgctgtat tcaattgtgt
    18721 actcgtatta gatacagctt caacaccagc tacagttgtt gcttgatcaa ttttagttgt
    18781 taagtcacgt ttttgtgcat tattaattga cgttaagtta tttaatgcat ttttagctgc
    18841 attcttcgca tttgtaacgt tttgatcacc attcaatgca tttttagcac ttgaaacatt
    18901 ttgaatagcc gcttcaacat cttgtttaga cgtatttgca ccttgcgttt tattcagaat
    18961 tgcttcagct ctgcttaccg catttgtata cgcatcacgt ttagctttat ctgcatcagt
    19021 aaatttaaca ctttgtttaa ctgtgttttg atcatggata gcttgttcca agttacccat
    19081 ttgcgtattc aactcagttg ctgcattttt agtagcagtt acctcactca catgacctgc
    19141 acgatcaatt tgtgatgaga tggcagattt ttggttattt gttaagtgtg ataatgtatt
    19201 taagttttgc ttagcagtgt tttgtgcatt tcttaagttt tcagcaccgt ttaaaccatt
    19261 tttagcatta ttcacttgtg ttgtagcttg ggtaatcgca ctagtatcca tagtcggatt
    19321 gttagtttga ttaatgatac ctttcgcatt tgacacagcg ctattatatg ttgattggtt
    19381 attcggactt gcgtctgtat agttttgact tgttttgact gttgtttcgt tagcaatact
    19441 atctcttaag cctttcattg cattgttcaa tgcttgtcca tttgtttgaa cagtttgtac
    19501 gtcttctaat ctattggctt gtcccacttg ttcttttaat ttagcttttt gaggtgtatt
    19561 taattgtgtt aatgcatcaa tagcagtatt ggcgttttgt tttgcttcac gtaatctttc
    19621 gtcaccattt aattcttgtt tcgcgctgtt tacttgatta gctgcagctg ttacttctga
    19681 aggtgtcgta acaaccgttg gcgtaccgct aataatatgt tcagcattgg taactttagt
    19741 tgtgtaagca ttttgtttag tgctatcggc attaacatat ttactgcttg ctaacgtatt
    19801 cgtcttatct gcgatggcat gttttaatgt gcccattgcc gtgttcagtt cagtcgcatt
    19861 gtgctgtaca tcttgtgcat tagatacgcg ttgagcacca ttagcttgtg cttttaatgc
    19921 atctttttgt ttttgattta aatcagatgc gccattgatt gctgtaatcg ctgcttgttt
    19981 cgcattgttt aagttttgcg taccatttaa tgcatgtttc gcattattaa cattattaag
    20041 tgcttgttcg actgctgttt tcgctgtatt cggtccagtt tgtttattta aaatggtttc
    20101 ggctgctgat acagcttgat tgtatgcatt acgtttttgc tcatcagcat ccaagaagtt
    20161 ttggctcgct aatgttcctg acttatcgtt gatagccgtt tgtaagttac ccatagcacc
    20221 atctaaacta ttcgcatttt gtttaacaga ttcaacacca gctaagtttg tagcttgtga
    20281 aatttgattt gttaaatcat tacgttgagc tgtagtgata tgcgttaaag tacctaaatg
    20341 ttgtttcgct gcgttttgag ctgcaattaa ttttgcatca ccattcaatg catctttcgc
    20401 attattaaca cgttgtaatg ctgcttcaac tgcattttgc gcagtgtttg gacctgtcga
    20461 tttatttaaa atcgctttcg ctgcagtaat agcattatca tactcttgtt gtttatttgt
    20521 atcagcatca cgatatttct cagatgattt cacttgagat tcgttgttaa taccattctg
    20581 taagctagcc atagcttggt ctagatgttg cgcattttgt tttacagtat caacaccact
    20641 cactcttgtc gcactagtaa tttgactaat caaattgttc ttttgagcat ctgtaatact
    20701 tgtcaatgtg tttaagtacg ttttcgcatt ttgttttgca gcagctaagt tttcatcacc
    20761 gttaagtgcc gttttagaac tattcacttg ctctgctttt tgtgtaatcg tacttggatt
    20821 catttctgga ttactattag cattaataat cgtttcagca gcagctactg cgttgttata
    20881 tgctgtttgc ttatcattat tagcatctac gtaatcttca cttgctttag tcgcatcttt
    20941 gttggcaata ctttgtctta acgtattcat ggcttgatct aatgtattgg catttgattg
    21001 aaccgtttga acaccagcga cagtagtacc actattaatt tggtttgtta aattcgtttt
    21061 ttgtgcagtt gttaaatgcg tcatattatt taactgctgt tttgctgttt gcttcgcttg
    21121 atctaataaa cgcgtaccat ctaagttatt tttagcagaa ttcacttgat tcatcgcttc
    21181 agtaacttga tctttcgttt tattttgacc atttgattta tttaaaatat ctttcgcagc
    21241 ttgtaccgca tttgtgtatg ctgttttctt actaggtgtc gcatcttgat agttttgact
    21301 attaagcgtc gtttgttcat cattgattgc accttgcaag ttacccatcg ctgtatttaa
    21361 atctgtacct tgttgtttaa cagtatttac gccagcaata tttgatgcag cattaatttt
    21421 agatttaaca tctgcttttt gtgcattatt taaatgtggt aatgcatcaa ttgctcttgc
    21481 agcatctgtt ttagattgtg ctaatttttc ataaccattt aatgcatttt tattagaagt
    21541 aacagctgaa gttgcacgat taacatcatc aacagacatc gttggatttg ttgtttgatt
    21601 aatgattgtt tcggcacttg aaactgcact gttatatgca ttttgtttag ctgtatctgc
    21661 atccgcataa tcttctgtac gtttcacttg atctttatct gcaacagctt gtcttaagtt
    21721 acccattgca ctattcaagt ttgttgcatt ttgcttaatt gtattaactg catcaatttg
    21781 atgcgcacca ttaatttgcg attgtaagtt ttgacgttgt gcattattta aattgtttaa
    21841 atgacctaat gcagtattcg cttcttgttt cgcagcattt aacttagctt caccattcaa
    21901 tgcatgttct ttacttgtga catttgataa agcattgtta acatcacttg gtgttataac
    21961 tggatgttgt gcattaccat taataatgtc attcgcatgg ttgtatgcat tgttgtaatc
    22021 atttttctta ttagtatctg cgttgacata attatcactt tgtacgactt ggttatgatt
    22081 agcaacgcca cgttttaaac ctgtcatagc agtatttaag ctttgagtcg tttgtttaat
    22141 atcatttact gcttgcacgt tttgtgcact attcacttgt tgtgttaatg cattcttttg
    22201 tgcttggttt aaatcacttg catgcgtaat cgcatttgtt gcttctgttt tcgcacgttg
    22261 taagttttgt tgaccatcta aagcatcttt cgtcgattta acagatgctg ctttttggtt
    22321 cactgtgtta acatccatcg ttggattagt cgtttctcca atgacacctt tagccgcatt
    22381 aactgcatta tcatacgctt gtttattagc tggtgttgcg tcagtgtaat tttgatctga
    22441 tttaattgta ttattatccg caacactgtc ttttaatttc gtcatcgcag tatttaaagc
    22501 cgtaatgtta gacttaattg tttcaagcgc agcatgattt tgagcagcat taatttgttg
    22561 cgtgaaatta ttttgttgtg cttggtttaa gttagatgca ccatgtaatg ttgttaacgc
    22621 tggctgtcta tctaatgtta aaccttgacg tgctgtattt aattgatcct tagcttggtt
    22681 tgcctctgtc actttatcat tgatattttg gacagttggg ttgccattca acacttgatt
    22741 aatttcagtt aacttttgac gcgctgcttg taatttttgg ttgtacgcat ttaacgaagc
    22801 ggtcgtcata cctgttgtat ccgttggttg attaatgctt tgttctaatt gcgttttcgc
    22861 agtttgaagc ggcgctttat ctggtgttaa agcttgacgt gcatgatcta aatcagattt
    22921 agcttgattt gctgtagacg tatttgtatt aatttgttct acagtcggtg aacctgctaa
    22981 tacttgattg atttgttgaa tcttattacg tgcagctgtt aacttcgcat tgtatgcatt
    23041 tatagagtct tgtgtcatac cagttgtact tgtttgcgtg tcaatactat gttgtagttg
    23101 atttttcgca ttttctaaag gcgctttatc gactgttaag ccattacgtg cttgatcaag
    23161 tgctgattta gcggcattcg ctgctgtcac gttttgacgt attgtcgcaa catctggatg
    23221 tgaggctaat acacgatcaa tttcttgaat tttagttcta gctgctgaaa gtttttcatt
    23281 aaatgctgca atagatgcgc ttgtcatacc agtcgtactc gttggctgat caatatcatt
    23341 ttgcaactga gtttttgcag tttgtaatgg tgccaagtct ggagttaatc cagcaattgc
    23401 ttgttttaag ctattatatt tttcttctac ttttgttttt tctgcggcaa tttgttggtc
    23461 agtcgcatca ccattgttaa taacattttg tgcatttgtt gattctgttt gacccgcacg
    23521 tttagcattt tcatatgctt ggattgatga ttgtgtcata ccatcagtag ttactgattt
    23581 attgatttct tcatcaagtt tcgtcttagc agtttttaaa gcactattat cagctaaagg
    23641 tactaattga ttaattgctt gcgttaatcg ctcattgaca cgatttacat ttgttaacgc
    23701 agattgcact tcttgtactg ttcttattgg cttttgaata atagcattag cgctattttt
    23761 agcacttgtt aagtcacttt gaagtgcacg aatcgaatta ttgtaagcag taatacttgc
    23821 cggcttctta ccagtcgttg tacctgtgcg attcaattgt tgcactgctt gctctaaggc
    23881 atgtgtatct gcagttaaat catgtttcgc ttggtttaat gctgttaatg cgttatcgac
    23941 acgatgtttt tcatctgaaa tttgttgtgc agttgcatcg ccattgtcaa taacacgttg
    24001 agctgcagtt atttcagttt ctgcttcacg cttcttcgca ttatagttat caatactttg
    24061 ttgcgtcata ccagcagttg atggtacttg gttcacagaa ctttgtaagt tatttttaga
    24121 cgttactaat tggctattat cttctttatt ttgaagtaat gctttagctt gatcaatctt
    24181 agtttgtgct gcacgaactt tagttagtgc gtcagaaact tgttgtggtg ttgcacgctc
    24241 attattaatc acttgttgtg cttctgtttt cgcagtattg atttgttgtt gcgcattatg
    24301 cattgcatta ttgtactgcg taattgtacc tggcttttta ccttcagtgc ttactggatc
    24361 atctaaatga tttttagctg tgattaactc acgtttatcc gcttttgtga aaatggactc
    24421 ttgtacttct tcagtactac catcattgta agttactgtc acaggaatcg tcgttgtgct
    24481 accaccagct aaattagtag gcattgctgt gccattttta atcgttgcag tacgtttatt
    24541 agcaacttga actgcattgt taatttcagc tgctgttaca tttgaaccat aatctttcac
    24601 aatttcagtt gtgttgacag tatgagctgc cggtgcagtt aatgtacttg gattactact
    24661 tactgagtga cctgtacctg ctttcggagt aattgtgatt gatgaatttg gttttatagt
    24721 attggcattg aacgtcactt taccagtttg tgcatctaac gttacatagt caggcttatt
    24781 cgcaattgtc cattgattat tttgaccacg aacaacatta attgtcttac tatgttctgc
    24841 accattaccc actttttcag tgtaagcaat atccattgct tgagttggat taattaaatg
    24901 tcctgatgga ttattaggcg tgatatcaat atgaccattt tgccaaatct tagtagtcgc
    24961 ttggttcggt tgtggtgcga caactgtgaa atcatcacta cgttgctcat cactcactgt
    25021 ctctccgctt ccttgcgttg caacaacttg aattgtatca gcagggttga aagtacctgc
    25081 tgcaacagta ataccattat tagttccagc aatacctgct acagttgctg cagatgcttc
    25141 tttcacccat ggactcgtat tattgcgacg tgtaaatgtc gtcacaacgt taccattacg
    25201 tttaataact aatttatcag cgtatgtcgt tacgttaccg gcatgtgtat tcactgtttg
    25261 gtttgctcca ggtgcaattg taatcgctcc tgccgctgtt tcagtcacag ttggtttcgc
    25321 tggttgcaca tcttttacta caaatttcgc tggtaaagat gttgcaaaag tatgtccgtt
    25381 atagatgacg tcatattttg cgttaacgac tttagccaca ttcggtttat tcatagctga
    25441 ccagtttgcg tcatttgtac ctgtagtatc acgattccat ttatacgtaa atccatctgt
    25501 tggtaaacca gtagcatttt gcatatgtgc atatcctgat gcttgcgtac cacttgctaa
    25561 agtgcctcca accgttgtcg tataagtagt ttgagggaat tcaaattgat atacattaac
    25621 agtaacagga actcgtttag cagctgaaat acctggatat gtgacatcga catttaaatg
    25681 ttgcacgcct gcttgttggt tatttggttg ttgtctattt gcccatgctg cagtgatacc
    25741 atttgtgttt gtatttggat caaatgtaat gtagttcatc gcatccgttc cattagtcaa
    25801 attttgacct ttcacatcac gtgatggcgc ctttgcatta gcaactggat aaactttgac
    25861 tggaacttca acattacgcg taccttgacc attaggtaat gttacaaccg cagttttatg
    25921 agtgttaccg actgtattct tccatgtatc tggactatca tgccatgcaa ccgttgcccc
    25981 atgtggcggg ttttgaataa atctttctac gtgtccgaaa tcaaaaccgt cgccaccttt
    26041 aataaataca gcgccttcag tagttgcttg taattgtggt gtcactgtta ctgttgcact
    26101 gtcatttgaa tcaacagatt catttcttgt tactgtaaca acttgaccat gttcgtcttg
    26161 cgtcgtatac gtcacattgt tcattgaaat tgaagatttt gctttaattc cgccatttgg
    26221 taacgcgtca cttactgtca caaccgaact aaaaccgcta ccatgagtaa tatttgtgac
    26281 atttaatggt gtattatctg ctttaaataa ttttactgac gagttattta atacgttatt
    26341 aactttaatt tcttggtttg taagacctgc tttatatgtc acagagtttg cgtcaattct
    26401 aggtgggtca ggtttaactt tagataaaat agttaaatct tctgtttgtc tattatcata
    26461 tgtcactcta ataagacgag tagatatgcc tacaacatct ttgttaggac catatgttcc
    26521 aacaaattgg aaattcatat gttgcgcatt tgtcgaccaa gaattattta ctggcttaac
    26581 atattgtttc gcatcataca tatctgaaac acctgggtat aaaacacctc tggctgtttc
    26641 aaagacatgt ttcggtacag ttcttactac tttatatgtt gctgttttcg taatcggcgt
    26701 tgtttcgcca tcaattaaga tatgtgcagt tacagttata tcttcaccaa tacgtgtatt
    26761 atctttattt ggcgcttgtc cacttaccca tgtaatagtt gcatctgcaa tgtcactacc
    26821 attagataac ttaaagtaat ctgatgcact agaaccatta cctgctggga agtcttggcc
    26881 ttgtacagtg taatgcgaat gtgcaacgat ttctggaatg acatgcttta caggtacagt
    26941 cactgttgat gttgttccat cttgataagt aacagttact gtgacattgg cattatttcc
    27001 agtacgactg acattactaa ctgttttact agtgatttca ttcgcacttg ctcttgcata
    27061 acttctattt ggtactgttt cagtaaacgt taacgaatta ataattgtcg tttgatcagc
    27121 ttgtgatact gtcgcattat tcgaaatatt ggcaattctc acaggattag cagccgttga
    27181 tgaagtacca actcgatatt tatcacgcaa aggtttcact gttacattga acgaagttgt
    27241 agctgtattg ccgcttgtat cagttgctaa taaattgatt gtcttattag ttgctgatgt
    27301 cacatttggt gccgttgcag aaacatgttg atggttatta tcaacagtac ctgtaatttg
    27361 tgatgtattt ggtacagtta cagattgcac accaaagtta tcattcgctg taatagtatt
    27421 tgtaaatgtt tcacctgaga acacttgatg attagtgtaa ttacctactg aaattgttgg
    27481 attcactaag tcacttggta caaaataaat gttaatagcg tcagtagtat ttcctgtatt
    27541 ttgatttaaa tgttcaacat attgttttgg accatatggc gttaagtata actgtgcttt
    27601 ataaactgca tcacttgcat tatgtgtaga attacttttt acaacgtggt caattgtaaa
    27661 tgcgccagca ccattagctg ccggttcgtt tacgttaaca acagttgagt tactatggtt
    27721 tgcacgagta tttgaatttg taacaggttg cccaccataa ccgtttgaag ggttgatgat
    27781 gtctaacact tgaatcactt gaccattcaa cgtaaattga cgttgaccat cagttgtcgc
    27841 atttgattgt gaataaccag tcgttctaaa gttaggtctt ccgccagctt ctgcttgtga
    27901 tttttcatta ccagtaatgt tatttcgcaa tccaggcact gtagttgttg cttttaacat
    27961 tgttaaaata tcattcgtcg taatttgagt acctaatgta gcatcataac gataaattaa
    28021 agatttatgg tcatcagacc atgataaacc accatctgtt ggtctgtttg caatttttgc
    28081 attcgtccat gtcactgtat aatcttgtgg gaaattaacc caacgcaaga aattcatatt
    28141 agctaggttt gacgcgaatg atttcgttaa tcgaccttta ttaatattta ctgtaatagt
    28201 acttacatta gcaccattag gtgtatttgt aactgaaata tcaccttcgg caagcgtaat
    28261 tacatctcta tttgcattaa taaatgcttg ttttactttt gagatttcat cagtagttaa
    28321 atcatatgtt gatgtacgat atacacgttg atcgccttct ggttgtttca catcgatgat
    28381 tttcaaacta atatagaagt cttcactatc tttatgatac gcatttgtag cagttatatg
    28441 gtataactgc gtgcctgcgt tcatttgact aacttcttgt gcagttggaa tagttctatc
    28501 agatgtaata tttgtaaatg tgccacttgg aataggttgt ccattactta aagtaaaata
    28561 ttgtttaata tcaccaatac taaatgattg tcctttagca atcgttaccg ttgctgtttt
    28621 agcagcattg acagtttggt attcaggttt cacaactggt tcgtatgtca tcacatttgt
    28681 agcatcaagc gttgtcgatg agccatcacg gtaatgtaat gtgacattac cattattatc
    28741 gacagttact ggatcagttg atgctaaata tcctcttata ttttggtttt tattcataaa
    28801 ggcagtaatt atgctttgct tttcatcatt agatacagca gtcggattga ctactacaac
    28861 tttctcagta tttcctaata caatcggatg agcatcttca ctaattttac ctacatgaat
    28921 tgaaacatgt ttagattgac tatcatttgt cgtattattg acattgtctg tcgctgacac
    28981 tttaaatgta atatcactgt taaatgcctg attcatactc actctacctg taatagataa
    29041 tgagccatta ttcttatccg cttttgttaa atttgatgtc caaccattcg gcaatgtcgt
    29101 aatagttgta ttttttatac cagaatgtct atcagagaca cccattgtaa agttgacttc
    29161 gtcgcctcga tagtatttgg catttattcc aacaggatta ttgattgttg gtggaatgtc
    29221 atctggcaaa atagtaacag cattactcat attagatgca actactttat tataataaac
    29281 tactgctcta atttgtttac ctacaggtgg tatgaataca acataatctt gcggtcgtat
    29341 agtaattgta ttatgcaccc catcaggtgt accagtcgca agtatcgtat aaccagatgg
    29401 tatttgatct gcctcactac caccattcgt agtttgattt gttggtgcaa ctaaataaac
    29461 caatgccgat ggatccaacg gtattcccga aatattaaca ggcacttttt gtaatgctgt
    29521 acctcttaat tgctcagctg tcgttgtaat tgttggtgta tgtggtttaa cacgaatttc
    29581 gaataattgt tctttacgtc caaaacgtat cgttccaaag taatcccctg gtaataagtt
    29641 accaacaagt tcaatattgc gttctctggc attattaggt ctatttgtcg agttttcaat
    29701 gtgtaatgta cgcggaatac ctgtaatttg ccaatcttta tctgtaaaat tctcattact
    29761 tgttaaatta atacgtaatg gttgaataga tgttacgcta ccacctaaat tcacggaagc
    29821 attcccttgg ttacctgcat atacatagaa aactggatgc ccagcttgtt ttgaaaatgg
    29881 tttttcagca atattttgag tatcgtcatt tttaaagaat ttaatattat gttggatttc
    29941 aggtaatacc gttaagttaa caggaactaa agtttcacta ttaccataag catcaggata
    30001 gtcgaaacca atcattaatc ttattgggtt tctagttctt gctgcttctt gtaatttttg
    30061 atcagtaaat aattgactta attgatattc atttaattgt tgggctccac ccggtctaaa
    30121 agcattagta tttgcattat tcgaataaaa tcctaacact ttattactcg gcgtcttaac
    30181 ataacttaaa ggatcatcaa caaaatcatc tatatttagg tttgtacttt ctaatacctt
    30241 tttcgtttgt acatttcctt gactcacttc tacacgttta gttgtaccta tgcgatatgt
    30301 acgattaacc acttgtctta aaccattaag acgttgttga tggtcttgcg ttgattcgac
    30361 gtatagttga ctgtacataa ataattgatt gtactctaaa gcacgcccac ctgctgcata
    30421 gtacaattta ttaacagttg gaccttgtgt tttaaatgaa attgtataac ttgcatcacc
    30481 ttgaccgatg aaagtataaa ctttaggaac acccggtgat tcagcaggtt tttgaccatt
    30541 aatatattca aaattttgtc taccaaaagc tttagtagca ggtgtcgctc cagcttcact
    30601 aaaataatca ctcgctctag ataaatcttc taaagaataa atatggacag ttggccagtc
    30661 atatattact tggccacttc tatttcttat tttaaagtca tgtgaacgat gaggatcatt
    30721 tactccatat tcccacattt gttgaagtgg tttatttgca cctgctcctg ctccatgact
    30781 ccattgtaca tttgttccat ctgaattaac tgttacaaag tcagttcttc ctactggcac
    30841 ttggtctgct ggtaatgcaa accaaaagat catattttga tgtggagcat gtcctttatt
    30901 aaacttaata acccattggt acccgtctcc agtttgtttc gctgtcataa gagcaatttc
    30961 ctttgagtca gaggccttca atgtattcca tggtgtagtt ggtaaattat atggcgcccc
    31021 tttactagaa gcattgatat atgtaccaac aggatagccc gaaccatcat tagcattttc
    31081 aacataatat gatgaatttg cattttgata gtctgatact gatctaggct ctgcagctct
    31141 tgactcaacc gaacgcgttt gaattctatt tgaacgtcgg ctagtctgtt gacgttgagg
    31201 cattgtacga attaaagcaa cagcttgttc caattctttg tatgcaagtt gcaagtcttt
    31261 attgtttatt ggatcattac tgcctaataa cgtattaatc ttatcaaaaa tgccttttac
    31321 atagttaaat gtgccagaat ctgcattttc acggtcaata aagcgataat catgataatt
    31381 tgcatcaaaa aacgcttgta attcatttcg gtcatgttgt acgttaccat tatcagatga
    31441 atctgaagct gttactacat ttgcttcatg attttctaca tgagccgctc tattgtcgtt
    31501 tccattttta ttagcagatt gactttcgtt ttgtttatgc ttactttgtt ctttatctgg
    31561 ttgtgttgtc gcagccgttg ccgaatcttt ctttgtattt acattttgag atgctggttg
    31621 ttcatcatta gtagtagatg attgcgctac tttttgatct actaaattag cttgactaat
    31681 atttggttgc tcattttcat gagtagcaga taatgattga ccattttgtg aattttgaga
    31741 attttgtact tgagattctc gattctcagt ctgttcatta ttactttgtt gtttctgttt
    31801 aaccacgctt gctggttgat ttgtttcagc agcatgtgct tgtgatgtat tgaatcctaa
    31861 aaataccaat gtcgcaatga cagttgaaaa tgtaccaact gtatatttac gaatactaaa
    31921 cttttgaatt ttatcacgat aattcacagc tattgttcac tccttatctt gttgttatgt
    31981 ctctgttgtg ttgtgaatgt atttactttt gattgcttgt gtattgtaat gctcattcca
    32041 ttctcattta aaaatttaaa acgatgacga ttcaatacaa tttcgcaatc tatccaaaaa
    32101 gtattgaaac acattacaac tatgtgttga acaatttatt tatgctccag aatataataa
    32161 cacaaaatat attgtaaggt aaattgaatt tgtgacaatt tataataatt ataaataaag
    32221 tagcgtaaaa gagtaataaa aaaccaatcc ctatgttcat ttaaaataat gacacataag
    32281 aatcggttta tatttttgtt taatattgta gcgacttgta tgatttcaat ttaattctca
    32341 attatcataa ttgagtatcg ttttgtttag gcacaagtaa caaaatgata acgaatgata
    32401 atattcccat acctgcattt aaccataatg caatcattgc acctgtataa atgtttgtca
    32461 tatttgatac gattgcatat actgcaccac tcaatgcgac gccaaatgct ccacctaatg
    32521 cagaagccat tttatagata cctgcagcaa cgcctacttt ttctaacggt gcatttgcaa
    32581 ttgctgtatc tgttgatggt gtagcatata tccctagtcc taaaccaaag aataaataac
    32641 ctataataca acaaatgaca tagaatattt ctggcaagaa agttaatgaa atgagacatt
    32701 ctccgacaat aagaactcct gttccaatta acattggttt cttgcatccg agtgtttgaa
    32761 gtaacttttc accaacacga atcataatta gtaccattac taaataagtg attgataaac
    32821 ttcctgcttg caatgaagaa tatcctaaac ctctttgaac aaatgtgttg gctactatta
    32881 atgttcctgc aacaccattt aacaaaaagt ttgaagctgt tgcacctgtg taagctttat
    32941 ttttaaataa tttaaaatcg attaaaggat ttgtagcacg cttttcaaga actataaata
    33001 aactaaaaga tccaattgca atagctaata aagtaataaa aagaagtgag gttacaccta
    33061 attctgatcc tttagtaatt aaaatattta aactgaggag cataatgact aaaagaacca
    33121 gacctttaat gtcaaattta tttagagaaa tcgatttaga tttagtttca ggtgtgcctt
    33181 taataagaaa cagtgcaatt aatgaaatta taattgatag gatgaaaatc caacgccaac
    33241 ctaaaagcgt tgcaactgca cctccaaaaa atgaacaaac accagagccg ccccatgagc
    33301 caattgacca ataacttaaa gcgcgttgtc tatctttccc aatgtaatat gacttaataa
    33361 tagacaaagt tgcaggcata atacatgctg ctgaaagtcc ttgaattaat cttcctataa
    33421 taagtaataa aggaatattt gaaataatga ttaataatga acctaatata tttaagataa
    33481 taccaatgtt cgtgagttta attctgccat atttatcagc aagaccacct gctcctacta
    33541 caaacattcc tgaaaataaa gcagttatac taacggcgat attaaccgtt ccaatatctg
    33601 tattgaaact atcttcaagt attggtacaa cattaaccaa tgattgtgca aatagccaaa
    33661 acgttattac acttagaaca attcctatta acaacttatt attgccttca aatgcctctc
    33721 ttgacggctt ttccatctct atttgcctcc ctatactttt gaaaaacaga caaattccat
    33781 aacagaaatt tgtctgttcc atagaataat tgaatggttt ttgattttaa tgtgtaaaac
    33841 tttaaattta ttagtagtcc atccatttat tctcaatgct agttatctta ccttatgctg
    33901 ctttttgttt ctttttatag taataaactg gtataccaag tgctgttatt agaattccaa
    33961 tgattgctaa tataaattgt gtaaacagtg tattaattaa tacaaatgat cctgccaaaa
    34021 tagcaattaa aggtatgatc ggatataacg gtactttata tggtcgttcc atatttggtt
    34081 cacgttttct taaaattatt accgcaacaa atgacataca atagaacaac caaataacaa
    34141 agattaacat atttgtaatt gtatcaaatg ctcccattga catcatgatg atagcgatta
    34201 taagttgtat aattgcgcca aaccatggtg cgccagattt tgttaatttt gcgaataaat
    34261 ggctaaatgg caataatttt ctttcagcca ttgcatatgg tacgcgcata ccagtcatag
    34321 tatagccatt gatcgtacca taaacagaaa ttaatatacc gattgtaata atcttaccgc
    34381 cattttcacc aaataatatt tttgatgtat ctgaagctgc atttaaatta ccagcaagta
    34441 gttctattgg caacgttaat aagaatgtag cgttaattaa taaatacaca gccataatac
    34501 aaccgatacc aactgaaatc gctaaaggta aatcgcgttt aggattttta agttcccccg
    34561 caacatttcc tacatgaatc caaccatcat atgcaaacat agttgctaat aaaccactac
    34621 caattgctgt aaagaagcca tttcctgaat tacctgtagt tggaattaat gaaaaagtga
    34681 tatctccaga ttgaaaaata ccaaaaatta caataacgat gattggaatc agtttaatta
    34741 ctaaagtaac tgattgtaaa attccgcctg cttttgaacc taggaaattt atcaacacaa
    34801 tagataacgc agatgcgatt gctattggta ttaacgaacc tatagataaa tggaataaat
    34861 taattagctg tgtcgcaaat acgatagaca atgctgctac gttagctgga aaataaataa
    34921 atgattgcgc ccaacctgat aggaagcccc agaaatcacc gtatgtatat tctatatact
    34981 tcgttaagcc acctgtttca gggattgcag cagcaagttc tgctgctgtt aaccccgcac
    35041 aaatggtaat gatgccgcct aggaaccata caaacaaggc cattcctgct gttcctgtta
    35101 cttctgttac gtttgatatt ttaaagaata ctcctgaacc aataactgtc cccataacaa
    35161 tagcaaacgc tgagaagaaa cctatatttt tttgtaattc tttaccattt gacataatta
    35221 cacctcattt ttaagattaa atgttttaac ctaccacacc cttgcttgta tctgcaatat
    35281 tcagtccatg ttcaatgaca cctgaaactc tagttaagtc aacattcccg cctgaaacta
    35341 atgcaacaac atttttatct tcaagccatt tattgtttat ttttccactt aaaattgcag
    35401 ctgttggtaa tgcgcctgca ccttcagtaa taattttggc acgctgcatt aaatctttca
    35461 tagcatgttc aatttcttct tcagtaacaa gaataaattc atctactaaa tgtttaacta
    35521 cttcatatgt ttgttcacca ggaactttta catcacaacc atctgctatt gtgctatcca
    35581 ctcgatgttc agttaaatct ctcttataga aagactcagc cataccatga acattctcag
    35641 attgaacacc gataatatga attgaagggt taaatgattt taatgcggtg gcaatacctg
    35701 caattaatcc tccaccgcca actggtacga tgactgtatt cacattccaa atatcatcta
    35761 aaatttctaa accaattgtt ccttggcctg ccattacaaa cttatcgtca tatggatgaa
    35821 cgattgtcat gccattttct ttcgctaatt cttccatata aagtctagtt tcgttaaagt
    35881 ttttaccttt taaaataacc tttgccccat agccttttgt tgcttgttgt ttcgcttgtg
    35941 gtgctgtttc aggcattaca atcgttgcat caatgcctaa taatttagct gttaaagcaa
    36001 caccttgtgc atggttccca gcagatgctg cgataatgcc tttttctttt tgttcatctg
    36061 ttaagtgatt aattttattg ctagcgcctc taaatttaaa agatcctgtg aattgcatat
    36121 tttctaattt tagaaataca ttccctttag ttatactttg gcttaaatac attgatttaa
    36181 ttagaggtgt tcgacgaata aatggtttaa tgcttgcttt agcttcttcg atatctccta
    36241 aacttacaat atgtgctgtt tgtaatgtaa ctgtgttggt tgtcataata attacatcct
    36301 tccgttttgt aaatttttag tccaaactat aatgctatat ttaaaatatt catttcactc
    36361 aatttcattt aaattactat ctattcgata acatttaata tttctttata atctaggtca
    36421 tgtgatgaag ctaatccttg atttgtcact tgtccttggt aaatgtttac accagtactt
    36481 aaggcttcat tatctttaat tgcttgttct aagcctttgt cacaaatttc taatatataa
    36541 tcaatatttc cttgtgctaa tgccattgtt gaagttcttg ggactgctcc tggttgattt
    36601 ggtacaccat aatgaatcac accttcttct tcatacactg gatcagaaat tgtagttggt
    36661 ctaattgttt caatagttcc accttggtca atagctatat cgattaatac tgaacctttt
    36721 ttcattgatt taaccatctc acgagtaacc aattttggcg gtttcgcacc tggaattaaa
    36781 attgtagaaa taaatacatc tgctttctta atttgttctg ctaaattttc tggtgttgat
    36841 ttgactactg tgacatcttt ttctgcatac atatcttcaa gatatttaat gcggtcatcg
    36901 tttaactcga taatgattac tttagcattt agtcccaagg caacatttgc tgcatttgtt
    36961 gctgctactc caccaccgaa aatcacatat gtactaccag gtatatccac attttcatgt
    37021 acaccagtca ctaaagtacc ttgaccacca tgttgtgctt cagagtagta agctcccata
    37081 attgctgagc gttgacctgc tatagcactc attggcgcta ataattctgc ttttccattt
    37141 tttataatgg tttcaccact aatcgcagtt acaccaactt cttgcatttt ttctactatt
    37201 tcttttgaag atgctagatg taaaaatccc cagataattt gattcttttt gaaatattga
    37261 tattcgcttt catgaggttc ttttactttg ataacaagat cagcttccca tgcttgttcg
    37321 tgagttacga tcttagcgcc ttctttttca tacatatcgt tagaaaatcc tgaaccaatg
    37381 ccagcatttt tttcaacaat tactttatgt cctgcatccg ttaacttacg cacattttcg
    37441 ggtgtgcaag ctacacgtcc ttcaccttgt tttaattctt tgactactgc aactaacatt
    37501 tgcaacactc ctctttcatt tgtgaataat ttcacaatta ttttatcctt tggttatgaa
    37561 tatgtaaata ccattaatta attattcata ttttatctat aatttgttaa taaatattta
    37621 atatattatt cttattctta aatattatgt gcaattatac cactatattt atttcttata
    37681 ctccatttga tttatagttg tattaataca ttgactcaaa aactaattaa tcaaatatgt
    37741 tttttagatt aataaagttg taactgtact attttgtagt gtatggtaat ttatttggat
    37801 gaaatataat tctttatact ctattgatta atcaaatatg tatctatcaa aattcggctt
    37861 atttattcac tctgacatat ccaaatacgc aaaaagacta tttctacctt gtatcgatag
    37921 aaatagtctt tttatactta attggtttaa tttttcataa aatcttatac tttttaaaaa
    37981 tgggatgaaa tatattttcc tgaaacatgt aattcatgtt cgttacaaat tgataaaata
    38041 tgattaagcg ttgttgcaaa tgacattttc tcaaaaagcg cttcactatc aattggtact
    38101 tgtgtgtgaa tttcagccag tcgcttcgat aaatatagtt catctaaatt gtcattaatc
    38161 ttattacgtt gcccagctga taatgcatca atgttttcaa ccacattttc aacgctttga
    38221 tattgctgaa ttaacttaat agccgttttc tcgccaatac ctttaacacc cgcatatcca
    38281 tctgctgtat cacccataaa cgctttaata tcgattagtt gttgtggttc aagggcatat
    38341 tcttcgttaa aacgatgtaa tgtatatcta ttataaatgt taaaaccttt tttaattagc
    38401 caaacttcaa cattgtcatt aatacattgc agtaaatctt tgtcgcccgt aataatatag
    38461 acatcgttat cagttgaata ttgttgtgct aatgtaccta taacatcatc cgcttcatag
    38521 tttttaacgc caatatttac aaagccaaat tgctctgaaa tttctttaac ataatcaaat
    38581 tgtggtatca attcttctgg tggtgcagaa cgattttgct tataaccatc aaacatatca
    38641 tttctaaaag ttgattgtcc catatcccaa catacagcta catgtgtagg gcgtatttca
    38701 tgtattgccg aaaagatatg acgcacaaat ccttgtattc catttgtagg ttcaccttgt
    38761 gaattgtaca taaattgttt atgaagactt gtagcataga aatgtctaaa taatagcgcc
    38821 ataccatcta caagtaatat tttattaggc atttaaaacg ttccttcttt cttaatataa
    38881 aatagttttc agttgttgat gtttatcttt caattgattg ataagtgttg tatctaaatc
    38941 aaatgctaat aaatcgttta tttgcgtttg aatattagct tcaaaacttt caaattgcga
    39001 ttcagcttct acagccattt gctttacata taattcaagc tgttgcctca ataatcccat
    39061 ttgtggttgt aataattcta acgtactttg acaaattgat tcatgtatat ctctttgccc
    39121 atttggattc aaaattttac gttttgttaa ttgtttaggc aatgcattta gcatatcgtt
    39181 gaaatcgatg tgcaataatg gttgctctat attagctgat tcaaagttaa actgaggatt
    39241 aataatgaca tgtaaatctg ctaattgttg aacgattggt gcaatttgct cagtgagttg
    39301 cttattaaag tattttttta tacgttctgt aattaaagat tgctctaaaa acaatcgttg
    39361 atgaatttga tctaagtata ctctcgtaga cactttcttt tcttcattaa aatcactatt
    39421 ttgcgtcatt tgagaattaa acactgattt aacatcgtca agtagttgta gttttaaacg
    39481 ggcatttaaa tgataaactt gttcttcaac ttcgttgtct gtatgttgta acgtcgcatc
    39541 aattaattga tgttgtaaac gctgtttatc tttataatct gttaaccttt gttggcgacg
    39601 tgaaatatca gctttatttg tttcaaattc tgtaatcatc tctacataag aacgatccat
    39661 ttgttgaagc tgatgaatca tttgttgttc taaaattgat ttagattcaa catcaacaaa
    39721 ttgttgtatg ctttgtttta attgatcaat gcccttatct tcagcttgca atgcatttcg
    39781 acttgataca gcaaaaatgt ctgattgtaa gtgtacttgt cttaatgcat ctgatacata
    39841 tgtttcaact gcttcaagat catcttgact ttctgctaaa tcagcagcat taattaccat
    39901 ttttaatgct tggttttcat tcaactggtt catatctttc atgtgttcta taaacgcttt
    39961 gtcattatca gtaaatgaat gattaaaata acttacatac aatattaagt ctgaagaagt
    40021 taaaatttgt tcggtttcat ttgtatgcct ttggttattt gagtgtagcc ctaatgaatc
    40081 aacaattatt ttacctttta accaatcatg cattaatgca atgtgtactg tcttaacaaa
    40141 tgttgcatat tcatcttctg cactccactt tttcaattcc tgttgattaa tggcatgttt
    40201 ttctccattt tccaacatat tgacatacaa tttataatgt ttttcaactg cctgaataaa
    40261 agcgagttga tttttattta aatgtgattt taacttttct aaatctgaat taataaagtc
    40321 ttctatagtc gaataagaca tatcttggta ttcaactact gcattaattt catctaataa
    40381 ttgcgattgt gattttagcg ttatataact ctcgtctcca taagatattt ctgtagtagc
    40441 tgctgtagta ggatttggag aactgactaa aatatgctcg cctaataatg catttatcaa
    40501 actactttta ccagcactaa atgttccaaa tacacctatt tttattaatt tattatctat
    40561 acgtgttaat gtttcgtgga tatcttgctt tgtacgcttg aacaaaggca catccgaaat
    40621 tatatcaagc gctttttgaa tatctataga catatttgtt gtagctgtat tttgatttag
    40681 ctgctgatta tcacgattat cctgagcatt atcagtagcc acttgatatg ttgtctcttg
    40741 tcgacctatt aatttatcta gagattcatc taaatgaata tagtagtgac gataattctt
    40801 agtcgtcaat gactgacgca gctcatttaa ttctgtataa cgttgatatt cttttaaatc
    40861 atcactttct tctgttggta attcatctgc ctgcacattt tctattattt ctttaaaaat
    40921 tggtgttgat tgttgttcaa catatttctt aatggcttta accacttcgt ctgaaaatgt
    40981 aagtacataa gtattgctga ttgatgtttg tggttgatat aaaccttcaa tcatttctgg
    41041 cttaacgtca taatgctgat ttaatacttt atctgaagct tcttttttat tgataaaacg
    41101 cgtaacaaat gacatatctt ctcgcattgg ttgacgaatt tgttgattaa catgttcttg
    41161 taacgcatct gtcgctgtta ataatcgctg ttgttgaatt tgtagttttt tcttcttttt
    41221 attaaaaaat ccgccaacat taaagtcttg agacatactt tccaaataac ttcgtaacat
    41281 ttctctcata ttatgcggca taatgtatgc attttctaaa atatttttac gcttattctt
    41341 taaaaatgcc attaattcat ctggattatt taaaagttgt gcctcttcac taattgcttg
    41401 atgttgttga ctatttaaaa atgcttgttc aaactccgct tcttcgatac ctaaatcttc
    41461 tagtacttcc tgaatttcag actgaatgta atctagctga gcttcggtaa tgtattcaac
    41521 cgttcttgat gtataatcct ctattgtctc tctatgttga tctaatgaaa ttagataact
    41581 tgataatact tcaagttcat tttcagggtg atcaaattta gatacataaa aggtgcgttc
    41641 taatttaata ccccaatctg caattgattt ttcaactcga gatttaaacg tagagaatga
    41701 caattcatcg tcttgatgct tgtcaatttg attaatgata aacacaacag gtattccaac
    41761 atcatttata tgcttcataa acttaaagtt aagttcagat tgaacgtggt tatagtcaac
    41821 cgtataaaat atcatattac ttgtatacat atattgttct gttattgact gatgtgatgc
    41881 aacatttgaa tcaacacctg gtgtatcttg caacgtaaac ccattttcaa atttagctga
    41941 ttgaaaatta atttctacag attcaacgtc gacattttgg cgattcattt cccttacttc
    42001 atcataatta gataatttgg catacgtttg attcggcaaa ttagcaataa tatcgtgatt
    42061 gtctgaaact gacacaatag cagtattact tgtcgttggt acaggtgaac ttggtaagat
    42121 atcttgttca attaataaat ttatcagtgt cgatttacct gcagaaaaat gtccaacgaa
    42181 cgaacatgta tattgctgca aatatacttt cttaattact tggttaattg tatgtaaaag
    42241 tgcttcattt cgcgactttt caacttcttt ttttaattta tataaaagat ctaattgttc
    42301 tttattaatc atgttacttt cccctttggt tcgttcacat agaaatgctt gcttgttaca
    42361 acgaattcat acaatatcga tgtccattta aacatagatc gttaaatggc tcgcattgta
    42421 ttatgtttaa attttatcat agctgcttga taaaacatac gaataatcaa ttttcttcaa
    42481 acattccaac ttaaaagttc atttttctaa aaatcacaaa tcatcgttaa atttttctag
    42541 aaaataaata ttgtcacagt ttgttcacac acatcacaga attgacaccg tataatagcc
    42601 aattgggagg gaaatatgaa aaatacattt aatccattac aaagattaca tttctatgca
    42661 gcaatattta ttgctccact gttaatcact ttaaccattt cgggcattgg ttacttgttc
    42721 tttccagaag ttgaaaataa tatttataag aatgagtttt ttggtgacag tgatgtaaaa
    42781 acgcatcaaa cattaaatga tgcagtacat caagttgaac aacaatatga aggattcttt
    42841 gtaagtaaag ttagcatact tgatgaacca tataacaaac gaattacact gagtgatatg
    42901 gcaggaaatc aacgttacgt ctttctagat cataacaatc aatttgttgc agatcaaaat
    42961 gcgaaacata cgtattctaa tgtgatgcga agtatacata gttctttgtt tactgaaaat
    43021 actattatta attatttagt agagttaacc gcatgttgga tgatattcat gattttatct
    43081 ggtacttatt tactcattaa gaagcattta atttctaaca aaagtaaggc acttcgttgg
    43141 caaaagtggc acgcaatgat tggagttatc attgcaattc cagtatttgt attagtctta
    43201 actggattgc catggtctgg ttttatgggc agtaaaattg ccggtatgat ggacacaaac
    43261 ggtgcccttg gtcaaggtga attagcgatt aatccaccta aatcagattt gaacgaatta
    43321 ccttgggcta cacgtaaaaa taaacagcca gcttcatccg aaaaaggttc aagtggtcat
    43381 catggtaatg cagcaatgcc tcaaaccaaa ttagattatc aaatatctat tgataaggtc
    43441 gttgaacagg cgcaaaaagc tggtattaaa aagccgtttt caatcgtata tccaagtgat
    43501 aaaaatggta cctttattgt atctaatact agtaattcag gtgttactgg gctagatgta
    43561 tcaccataca aggaacaaac actttatttc gatcaatata gcggtaaaaa gctaggtacg
    43621 attaaatatg atgactacgg tattattgct aaatggttta catggggcat tccgcttcac
    43681 gaaggtcatt tattcggcat tttaaataaa atcattaatt tatttgtatg tatcgcttta
    43741 ttagtagcca ttggcatggg gtttgtctct tggataaagc gtacaaaaaa tactgcaata
    43801 aaagtaccac atcgcgtaaa aaaaccagca tctatatcac tcataatatg tttaattgta
    43861 ttaggattat taatgccatt atttggatta tcacttatcc ttgtatttat aattgaatta
    43921 atattatata ttaaagatcg tcgtgctaaa caataatgca cttaaagttt tgaactgacg
    43981 aaatttacaa aatgattctc gtctctctaa ttacttaaaa cgggttctat aataaatcgt
    44041 actgatggaa agtttttact ttttatctgt ccgatttttt gaatttgaag ataaaaaaac
    44101 atctaaaacg cataagaaga taactctact tcttcaaaat tacgttatag atgctttttt
    44161 attaattcat atgcatgtcg tgaattttca tcattaaacc gtgtgggacg tcatcatgtt
    44221 taacaatctc ttgtttataa aatgtcttcc catcatcttt agaaactaat tttacaaagt
    44281 ctcctttttt cggcttgaaa tcattagatg gttctacttt gatattatgt ttgactaatc
    44341 catctttttc actgacaact ttttctgcat ttgtattact attcatataa ccaaaataag
    44401 tcgttttttg actagaaaaa atcattaaac atacaacggc aataccaata acaaccaatg
    44461 caatagataa acctagaccc atttttttac ccatttatag actccttcat ttttcaaaaa
    44521 tacctattat catgagaact taattattac taattttagt cacgcattag aaacacttta
    44581 gttttcaata gttttacgtt ctgtcttttt accccagtat tgataataag tacattctat
    44641 atatccatta aataacttac gtcgctttgt tgctttacga tctactaaat attcaaattc
    44701 tttattactt gttaaaatgt atgtagataa aaatggatgt tgtttcatta gtttaccaat
    44761 ataacggtac atttcttcaa cttcttcacg atcaccaata cgttcaccat atggaggatt
    44821 tccaattaac gccaccggtt cttctgtatc aattgttaat gtattgacat cttttacact
    44881 aaatttaata atatcagaca atccaacttc ttcagcgtta cgcttagcaa tctctaccat
    44941 ttctggatcg atatcagaag catatacttc gatttcttta tcataatcag ccatcttatc
    45001 cgcttcatca cggtaatcat cataaatatt tgctggcatg atgttccatt gctctgatac
    45061 gaactcgcga ttaaaaccag gtgcgatatt ttgagcaatt aaacaagctt ctatagctat
    45121 tgttcccgaa ccgcaaaatg gatcaattaa aggtgtatca cctttccagt ttgcaagacg
    45181 gattaaactt gctgccaacg tttctttaat tggtgcttca ccttgtgcta atctataacc
    45241 acgtctgttc aaaccagaac ctgatgtgtc gatagtcaat aatacattat cttttaaaat
    45301 ggcaacttca acagggtatt tggcacctga ttcatttaac caaccttttt cgttatatgc
    45361 gcgacgtaat cgttcaacaa tagctttctt agttatcgcc tgacaatctg gcacactatg
    45421 tagtgttgat ttaacgcttc taccttgaac tgggaagtta ccctctttat caattataga
    45481 ttcccaaggg agcgctttgg tttgttcgaa taattcgtca aacgttgttg cgttaaaacg
    45541 tccaacaaca attttgattc ggtctgctgt gcgcaaccat aaatttgcct ttacaattgc
    45601 acttgcgtct ccttcaaaaa atatacgacc attttcaaca tttgtttcat agcctaattc
    45661 ttgaatttcc ctagcaacaa cagcttctaa tcccatcg
//
LOCUS       DANRDI010000003        44869 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.003, whole genome shotgun sequence.
ACCESSION   DANRDI010000003 DANRDI010000000
VERSION     DANRDI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 44869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.003"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(229..1665)
                     /locus_tag="PZ377_000053"
     CDS             complement(229..1665)
                     /locus_tag="PZ377_000053"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDM6356946.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(1681..2472)
                     /locus_tag="PZ377_000054"
     CDS             complement(1681..2472)
                     /locus_tag="PZ377_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDM6356947.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            2621..3325
                     /locus_tag="PZ377_000055"
     CDS             2621..3325
                     /locus_tag="PZ377_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDM6356948.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(3433..4134)
                     /gene="lrgB"
                     /locus_tag="PZ377_000056"
     CDS             complement(3433..4134)
                     /gene="lrgB"
                     /locus_tag="PZ377_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDM6356949.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(4127..4570)
                     /gene="lrgA"
                     /locus_tag="PZ377_000057"
     CDS             complement(4127..4570)
                     /gene="lrgA"
                     /locus_tag="PZ377_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDM6356950.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(4683..5423)
                     /locus_tag="PZ377_000058"
     CDS             complement(4683..5423)
                     /locus_tag="PZ377_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDM6356951.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(5426..7180)
                     /locus_tag="PZ377_000059"
     CDS             complement(5426..7180)
                     /locus_tag="PZ377_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDM6356952.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(7427..8101)
                     /gene="scdA"
                     /locus_tag="PZ377_000060"
     CDS             complement(7427..8101)
                     /gene="scdA"
                     /locus_tag="PZ377_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDM6356953.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(8241..9965)
                     /gene="tarS"
                     /locus_tag="PZ377_000061"
     CDS             complement(8241..9965)
                     /gene="tarS"
                     /locus_tag="PZ377_000061"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDM6356954.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(9998..11686)
                     /locus_tag="PZ377_000062"
     CDS             complement(9998..11686)
                     /locus_tag="PZ377_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDM6356955.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(11708..12733)
                     /locus_tag="PZ377_000063"
     CDS             complement(11708..12733)
                     /locus_tag="PZ377_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDM6356956.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(12726..13442)
                     /locus_tag="PZ377_000064"
     CDS             complement(12726..13442)
                     /locus_tag="PZ377_000064"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDM6356957.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(13718..14887)
                     /locus_tag="PZ377_000065"
     CDS             complement(13718..14887)
                     /locus_tag="PZ377_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HDM6356958.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(15315..17009)
                     /locus_tag="PZ377_000066"
     CDS             complement(15315..17009)
                     /locus_tag="PZ377_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDM6356959.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(17031..18056)
                     /locus_tag="PZ377_000067"
     CDS             complement(17031..18056)
                     /locus_tag="PZ377_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDM6356960.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(18049..18765)
                     /locus_tag="PZ377_000068"
     CDS             complement(18049..18765)
                     /locus_tag="PZ377_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDM6356961.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(19293..20336)
                     /locus_tag="PZ377_000069"
     CDS             complement(19293..20336)
                     /locus_tag="PZ377_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDM6356962.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(20360..20506)
                     /locus_tag="PZ377_000070"
     CDS             complement(20360..20506)
                     /locus_tag="PZ377_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6356963.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(20508..21563)
                     /locus_tag="PZ377_000071"
     CDS             complement(20508..21563)
                     /locus_tag="PZ377_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDM6356964.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            complement(21581..22840)
                     /locus_tag="PZ377_000072"
     CDS             complement(21581..22840)
                     /locus_tag="PZ377_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDM6356965.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(23067..23345)
                     /locus_tag="PZ377_000073"
     CDS             complement(23067..23345)
                     /locus_tag="PZ377_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDM6356966.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(23368..23835)
                     /locus_tag="PZ377_000074"
     CDS             complement(23368..23835)
                     /locus_tag="PZ377_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDM6356967.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(23820..25916)
                     /locus_tag="PZ377_000075"
     CDS             complement(23820..25916)
                     /locus_tag="PZ377_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDM6356968.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(26047..26145)
                     /locus_tag="PZ377_000076"
     CDS             complement(26047..26145)
                     /locus_tag="PZ377_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6356969.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            complement(26255..27190)
                     /locus_tag="PZ377_000077"
     CDS             complement(26255..27190)
                     /locus_tag="PZ377_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDM6356970.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            27552..29081
                     /locus_tag="PZ377_000078"
     CDS             27552..29081
                     /locus_tag="PZ377_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDM6356971.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(29401..30354)
                     /locus_tag="PZ377_000079"
     CDS             complement(29401..30354)
                     /locus_tag="PZ377_000079"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDM6356972.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(30665..30772)
                     /locus_tag="PZ377_000080"
     CDS             complement(30665..30772)
                     /locus_tag="PZ377_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6356973.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            30927..32072
                     /locus_tag="PZ377_000081"
     CDS             30927..32072
                     /locus_tag="PZ377_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDM6356974.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            32098..32271
                     /locus_tag="PZ377_000082"
     CDS             32098..32271
                     /locus_tag="PZ377_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6356975.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            32428..32784
                     /locus_tag="PZ377_000083"
     CDS             32428..32784
                     /locus_tag="PZ377_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDM6356976.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            32982..34457
                     /locus_tag="PZ377_000084"
     CDS             32982..34457
                     /locus_tag="PZ377_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDM6356977.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(34771..35913)
                     /locus_tag="PZ377_000085"
     CDS             complement(34771..35913)
                     /locus_tag="PZ377_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDM6356978.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            36354..37931
                     /locus_tag="PZ377_000086"
     CDS             36354..37931
                     /locus_tag="PZ377_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDM6356979.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            37957..39462
                     /locus_tag="PZ377_000087"
     CDS             37957..39462
                     /locus_tag="PZ377_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDM6356980.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            39574..40785
                     /locus_tag="PZ377_000088"
     CDS             39574..40785
                     /locus_tag="PZ377_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDM6356981.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRVVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            40971..43232
                     /locus_tag="PZ377_000089"
     CDS             40971..43232
                     /locus_tag="PZ377_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDM6356982.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            43262..44446
                     /locus_tag="PZ377_000090"
     CDS             43262..44446
                     /locus_tag="PZ377_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDM6356983.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            44760..44867
                     /locus_tag="PZ377_000091"
     CDS             44760..44867
                     /locus_tag="PZ377_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6356984.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
BASE COUNT        14320 a         8341 c         7113 g        15095 t
ORIGIN      
        1 ggaacagaaa tgatattttc gcaaaattta tttctgtcct tccccaactt gcattacctg
       61 tagaatttct tttcgaattt ctctgtgttg gggccccgcc aacttgcatt acctgtagaa
      121 tttcttttcg aaattctttg tgttgggggc ccgccattat aacactgtag gtcctactat
      181 attaatttat gtctcgggct ctaaccctaa aatacttata atataccttt ataatttatc
      241 tccatttgat gctatcactt gttgatacca atagaacgaa tcttttttca agcgttccat
      301 cgtgccatga ccatcattat ctcgatcaac atatattaaa ccatagcgtt tcttcatttc
      361 accggttgta aatgaaacaa tatcaatgat tccccacggt gtataaccga ttaaatctac
      421 accatcttga tcaactgctt ctatcgctgc tgtaatatgt gcttttaaat attcaatgcg
      481 ataatcatca tgaatatgtc catctaccac ttcatcaact gcaccaaaac cattttccac
      541 aataaaaagt ggtaactgat aacgatcata taacacattc aatgtatatc ttaagccatc
      601 tggatcaatc gcccaacccc aatcactcgt tgcgatatgc ggattctcca cagaatgatt
      661 caaaccaccg ttgacgatgt tgttttcaac tgtagtatca acatcatgtt ttacagccgt
      721 agacatgtaa taactaaagc caatataatc aaccgtgccc tgctgtaaaa tactgtcgtc
      781 cccttcttgc catccaacat catatccttt tcgtgccaac atttttttag catagcttgg
      841 ataataacct ctcacttgga catccgggaa aaagaaacgt aagcgattcg caatttgtgc
      901 ttccatcata tctttcggat gacacgaata tggataaatg ggtacatgtg aaatcattgt
      961 tccaatctta aacttcggat taatctcttt accaagacga actgctaaag cactggctaa
     1021 aagttcatga tgtgctactt gatacaagac ttcttcagga ttatcatttt ctgtcaatgc
     1081 taccccagaa ttcgtccata aaaagatagg atttgatgtg tccatctgat tattaatttc
     1141 attaaacgtc atccaatatg taactttatc tttatatctt tcaaatacaa cacgcgcaaa
     1201 atgcacaaaa taatcgacaa cttctctatt tctaaatcca ccataatgtt tcgctaaatg
     1261 aagtggcatc tcaaaatgtg ataacgtcac aacaggttca ataccttgtg caattaattc
     1321 atcaaagata cgatcataaa aggcgagtcc ttcttcattt ggcacatctt catccccatt
     1381 cggaaagata cgtgtccacg caatcgacgt acgtaaacat ttcaatccca tttctttaaa
     1441 caaggcaata tcttccttat aacgatgata aaaatcaata ccttcatgat ttggataata
     1501 gtgattggga tctatagatt ctgtaatctg acgtgctttg ccatgtgcac cactcgtcat
     1561 aacatcaatt acacttaacc ctttaccacc tttatcatat ccaccttcaa attgatttgc
     1621 ggcaagagcg ccaccccaca tgaaattttg cggtaatttt gtcattatta caacacactc
     1681 ctagttttta aatattttaa aaataacatc ttcagtcgta atgatttcat tcattgtcag
     1741 ttctactttt cctaaatcgg cagagttgct aataacgaca ataacgtcag cattgtagcc
     1801 ttgttgttgt atatattgct ggtcgaattg caacaacgtt tgccctgctt caacatgatc
     1861 attttgcttt acaaagcact tgaacccttt accttccaag tcaactgtat tcaagccaat
     1921 atgaatgact atgtccacac cgtcttctga ttgaatacca actgcatgct tagttggtac
     1981 aatcatagat attaaaccat tgaacggtgc gattactttg gattcttcat gagctttgat
     2041 tgctaagcct tcacctacca ttttctctct aaaaattgaa tctcttactt ctttaagtaa
     2101 aatacgacga ccagcgattg gtgctttcac taaaattggt gttgttactt ttacttgagg
     2161 tgtataactt gtatcttgac tattaatatt gtcagtattc tcactctctt cgacattagc
     2221 attcttaatt aatcgctgta attgctgata cacttcatcg acatttccat ttaattttat
     2281 gactaactgc gtatcatttt ctgctacaga tgtcacatca actatttgtt tcacttcatt
     2341 tgctgtagaa ggaattgtat gttgcatatg aattgtaatg cacttcgtat catacgttgc
     2401 atcaacaatg ttttcgacac cacctacagc tgtaagaatg tcttgggctt gttctttata
     2461 tttattgctc ataaatatta ccccctcaac ttgatagtag aatgaaaatg tatttactta
     2521 cctccaatta taattgtacc ggttcaattt gtaaacgccg atacaattat aatattttgt
     2581 gctataataa ttacagacaa agtgaaacga ggacagaata ttgttaaagt atgaacatat
     2641 tgctaagcaa cttaatgcgt ttatacatca atctaatttc aaacccggtg ataaattgcc
     2701 aagcgtgacg caattaaaag aacgttatca agtaagtaag agtactatca ttaaagcatt
     2761 aggcttattg gaacaagatg gtttgatcta tcaagcacaa ggcagtggta tttatgtgag
     2821 aaatattgct gatgccaatc gtatcaacgt ctttaagact aatggtttct ctaaaagttt
     2881 aggtgaacac cgaatgacaa gtaaggtact tgtttttaag gagattgcaa cgccacctaa
     2941 atctgtacaa gatgagctcc aattaaatgc agatgatacc gtctactatt tagagcgatt
     3001 aagattcgtg gacgatgatg ttttatgtat cgaatattct tattatcata aagaaatcgt
     3061 gaaatattta aatgatgata ttgctaaggg ctctatcttc gactatttag aatcaaacat
     3121 gaaacttcgt attggttttt cagatatttt ctttaatgta gatcaactca cttcaagtga
     3181 agcttcatta ctacaattgt ctacaggtga accatgttta cgttaccacc agacttttta
     3241 tacaatgact ggcaaaccct ttgattcatc tgacatcgta tttcattatc gtcatgcaca
     3301 gttttatatt cctagtaaaa agtaataaat acataaaaac gtctatatcc cagttataaa
     3361 ctggagtata gacgtttttt tacgataata acaatggctc aaattgctat tatcttgctt
     3421 aggtttttcg ttttagaaga atattgctac aaagacaggc acaactgcta caacaactac
     3481 accaactaac actaaagcta tacttgccat tgattcttct acaggtccta attctttggc
     3541 tggtgctaca cctaatgtgt gaccacttgt tccaagtgct aatcctcggg caatagggtt
     3601 agtaattcgg aaaagcttta agaatttatt acctagggca taaataatga caccatttaa
     3661 aataactgct aatgatgtta attcttttat accaccgata ccagctgata ctggtaacgc
     3721 aatcgctgta gttgctgctt gaggtaacat tgataaaata acatcattgg caaattgtgc
     3781 taacttcgca aaagttaaaa taattaataa cgctacaact gtaccgatac caatacctcc
     3841 gatgatacga tgccaatgtt taacaagcac ttcacgcttt ttatataacg gaatcgcaaa
     3901 acagattgtt gccggttcta agaagaagta aataatgtct ccacctattt tgtaagtctt
     3961 atacggaatg cctgttaaat agaggaaggc cacaccaaat accatactga caaatagcgg
     4021 tgcgaataag aagaaacgat tagttttttc aaataatatg gtcgctaaga aaaatggtat
     4081 aacggataac agtattccga agtaaggtgt gtttagtgct aagtggttaa tcatgagctt
     4141 gtgcctcctc tattttgatc ttttttgtga ctttgtcacc tttagatctc gaagtaactt
     4201 tcataataat ttgtgtgaca tagccagtac aaataagtaa tagtattgtt gagacgatta
     4261 ttagtccaat gattaaaaat ggtgcttggc taatgacacc taaagagtta acaactgaga
     4321 taccggctgg tacgaagagt aagccaatgt tatttgttag tgtcgttcct actttttcga
     4381 cttcgcctaa cttaacagca ccagtacata ataatacaaa taataatact aaaccgatta
     4441 ctgatgcagg cataggaatt ggcataaatg attcaattat tttcgataca aagagtacta
     4501 aagcaattac aatgacttgg tgaaaaaagt gtgctggttt tgatgcgtct ttttgttgtt
     4561 tcacgaccat tgcctcctac gtttgattta actaaagtat agatggctca cttcgatttg
     4621 cgtgattttt agtccgaaat acaaaatatc ataggtaaaa tgcataaaaa aaaggattac
     4681 tgttaaagta atcctatcga cgctttaaaa tctttcataa atgaacgtcc aacttgcatc
     4741 ttgacaccat ttgtcaatat taccatataa gtatagttaa accattgttg cacttcttta
     4801 atgtgtttcg tgttaataat atatgaacga tgaatacgta taaaataagt gggattcaat
     4861 cgtttttcat aacgattcaa tggctctgtt gtttcgtatt tatgattcgt tgtatgtatg
     4921 gttgtaatac cattatgtgt gccaatccca ataatatttt gttgctttaa catgtgaatt
     4981 ttatcgtcaa tttcaacagg taagctttga tcaaaattcg ccgacatatc attcgcaatt
     5041 gcacttgcgt tattatcatc tttggcttta gtcgcacgca ctttattgac tgcttgttca
     5101 atacgttttt gaccaaacgg tttcaaaata tagtctgtcg catttaattc aaatgcctgt
     5161 actgcgtatt ggtcatgtgc agttgcaaaa ataatcgcag gtggctcttt catcttttga
     5221 atcttagctc ctaattcgat cccattttca tccattaaat tgacatctaa aaatataatg
     5281 tcatattgat tgatcagtag tgcttccaat gtttctttta cattttctgc ctcattaatt
     5341 tcttcaaaac caccaatttc atttaataaa tatgttaatt cattacgtgc taatggctca
     5401 tcatctatga ttaatgcttt catatttatt cctcctcttg tctttcataa ggaagtacac
     5461 accaaaaagt ggtaccgctc gatgtcgatt caaattgtaa tgctgcggat tttccaaata
     5521 atccttttag gcgtaagttt aaattttcta aagcactacc agttccagac tctgattcta
     5581 cagatgtttc tcccaacaaa tgcattttat ctttagaaat accctgacca ttatcttgta
     5641 caataatacg tacatgtgtt gcagtttctt taatcactga cacgtcaata tcgttacctt
     5701 gctttcgatt cgtaaacgca tgtttgatgg cattttcaac taaaatttga attaaaaatg
     5761 gtggtacaag cacatcgcga tatttgtctt caacattaat attgatatta aatcttcctg
     5821 gataacgtgc ttgttcgagt gataagtatg cacgcacttg acttaactct ttatctaaag
     5881 taatcgtatg ttgcttagag ccttgtaaat tcgctctgaa aaaataactc aattctaata
     5941 gtaactctcg tgccttttcg ctatttattc taactaaagc tgagatcgtg ttaattgaat
     6001 tgaagaaaaa atgtggactc acttgtgcct gtaatgactt aatctcagca tctttcaata
     6061 acttactttg cgtttcggct tcaccaagtt caatttggct actaaaaata tttgccaatc
     6121 cttctgcaag ttgacgttcc acaaaagtta aatcattagg gtttgtaaaa tacatcttca
     6181 atgtaccgac gatagaacca tgcatctcaa gtggtatcac gatagctgct ctaagcgggc
     6241 aattcggatg actacaacca atctcttctt tagtatgaac ttctttcaac tttcctgatt
     6301 tcaatacatc tttagacaga cttgttaata tttcatttgt tggtatgtga tgatcactac
     6361 ctgcacctac atgcgataag atttcatttt tgcttgtaat tgctacggca gatactttca
     6421 ttaaattttt aataatcatc gcaatttgct gtgccgattc tctattcaat ccttctttaa
     6481 aatacggcaa tgtctggttc atcaattgca gtacatcatg tgtttgaaca gccttcattt
     6541 gctcctcttg ctttaatgtt gaaatgataa tagacataaa aatcgccgta ccaacgctat
     6601 taacaataat cattggtagt gcaattaatg atatgaggtc aaccgcatat gctttgtcgt
     6661 gggaaaatgt taaaatgctc aacatttgaa tcatttccat aacaattcca atcatggcac
     6721 ttttcgcaat actcgggtaa cgcttgcgtc tttgagcttg taagccaaaa taaccagcaa
     6781 ttataccaat aaatatagat gagataagat aaacttgtgc atccgcccca cccatataca
     6841 ctctgaaaat acctgaaata acgccaacaa atagacctac aaaagggcca ccaactaatc
     6901 ctgcgacacc tatcgttaat acacgtgtgt tagctaaaga tacatcatca tctaaacgga
     6961 agtacacact tcctgacaaa ctatgttgat gatcgatgac gataccagtt aaattagaca
     7021 ttaaggcaaa caaactgaaa ataatacata attgccaacg tgctttccat gtacgtcgac
     7081 gattcattaa gtttttaaaa tatggaatat tcatcaacac ataggccaaa ataataatta
     7141 aacctacacg ctcaagtaat aacattgtta gcgatagcac gatagcacct cagtgaatta
     7201 taattatgtt tattttatca taatagtgca tgtaaaattc atgaataaat tattaaaaaa
     7261 acacgctact atttaattat ctataccatt aaattttcat atcaattttt atgctaagtt
     7321 gaccattttt cttttcaaat aatgctgttt atgtcactat tatgactttt atacatagcg
     7381 aaaaactgct ggacttattt ttgccaacag tttctaatcg cgttatttat gatacttttt
     7441 taaataatac gtgattctct aaatgtacgt gttcatgtgt taacacttca agtgctttta
     7501 atctctgata aacaagtcgc caagtaccac acgcttctat cggaggttca taactagatg
     7561 ttaattcgct catttttact aacaattctc ccgttgcaat gtggtctgaa actaaatcat
     7621 ctatcacagt attaatatcg tctactacct caccttgctc atatttaatg agttttggaa
     7681 aatcgacatc gtcttctttt tgcatatgct ctaacatgcc atttttaaat gtatcgtatg
     7741 tttcttttaa ctcaactaaa tatggatgat taggtccatg tactttcgat aacttcgtca
     7801 cataaggtgt taaattttta aattcttctc ttagaggttc atgatatgct gattgaatat
     7861 attgaataag tgatgaaaca tttaaaaatt taggatttaa cgaacctggt gtattcgttt
     7921 gttcaacgtc gttgagacgc tgtaataatt cgttcaaatc tacatttttc ttttctaagg
     7981 ctgctgcttc tatacttact tgtccgccac aacaaaaatc tattcccaca cttctaaaaa
     8041 tatccgctgc tttcggataa tcagttacta catctgctac tatgtcattt ttatttatca
     8101 ttatagataa cctcctcatt tccagtgtat acaatttaat gtatatgaaa atgaaaatca
     8161 gggaaatgtc ataatcatgt cataaacagc aaaataaagc cctcaaatca tgttggctca
     8221 caatgatttg agggcattta ttatttattt agtggataag tgatatgtgc agtgtttaac
     8281 ttaacaatat ttttgaagtc tcgcttttta atatctttgc taagtaagat ttgcacttcg
     8341 ttttctgatg tttggcttaa cattcccatc accatgtcgt cttttagtac gacactagcc
     8401 ataccttctt caaatacata accacgatcc agaccagtta aaatgatttt gtttttacct
     8461 tttaacttca atgagattga atccgttaat gtaatagctg taaagtataa cgaaatgtta
     8521 ttaaagtctt ttgtgaaata cggtgttaat ctataaaaat cattattcaa atgaattaat
     8581 gcacttgttt ctgcagtata tgcatattta ttacgttgat taccaacacg cacttcaaat
     8641 acttccgtac ctatttggac ttctaagaaa acatcccaaa ttttttcttt aggtaaatac
     8701 gctttaatat cttctaacat aatgttaaaa cgatattgtt tattcgcatt gacacttaaa
     8761 ggtacataca ttgtgtgctt cttgtttcgt gaatgtaatt taagtctgac atcataatgt
     8821 ccttcgccaa tcataaattc atttaacgtt agctctgtaa caattttatg attatcaaat
     8881 ttaaatgctg tcattttcac ttttggctta gcgatatcaa tgcctttaaa gtatggttcg
     8941 ccttcattga attgaatgtt taatttacca tctacaatat caaaacggta gtattgacct
     9001 tgacggtaac tttcctccac agttctatag ttgtctatat ctttcgctct cgcgtagtgc
     9061 aacaatggtc gtaatttact catcaccaat gcatctacac gttctggaac tgcttgtata
     9121 aagtctccta gagcattaat ccattgtggt tgatcttcta gtttaacttt aagtgagaag
     9181 ccattcgtac gagaaaaact aaaatgacga tttaaaaatt ctgctaagat ttgattttta
     9241 tgggcttctt ctaaatctgc atttaatatt tctacagcaa tcaatctcat gacttcataa
     9301 aagtcttcag gtgaaacata cgcactactc atatgttcac cttcacgctt tgtagcataa
     9361 taatacgctt tatcacttaa cacactgatt cgatttgcat ttaaatatgc tttcattgta
     9421 aataattgat cttctgcact ctttaattct tctggaaaat aaatgtcatt atcttttagt
     9481 aatgctgttc tatagatttt agtcgggctt aaagtataga taattcttga atttaacaaa
     9541 gtaacttcag gtgctgtttc tttaaacata gattgtggta caccacgacc attaacacct
     9601 ttcattttaa tcaataagac atctgagtga tgttcatcaa taaatgctgc tgcatccttt
     9661 aaagtctctt tgtttatata gtcatcggag tccacaaata atacaaattc accttctgct
     9721 tgttttaacg ccacatttct aggtttacct ggaccaccag aatttgtttc gagttgactt
     9781 actttcaagt tcaatttatt gcgatacttt tcaactattt gtaacgtttg atctgttgaa
     9841 cagtcatcaa ctacaaccac ttcaaattca gttttcggaa aatcttgttt cgcaaggcta
     9901 ttaagtaatt ctgttatata cttttctgaa ttgtatgttg gaactattac tgaaaatttc
     9961 atcattatac ctctcccact ttgactacta tataaactta gctaccaaat aaatttctga
    10021 ctaaacgctc acttgatcgg ccatcttgat atttaaaatg tttatctaag aatggaatga
    10081 ctttttctcc ttcataatct tcattgtcca aggcgtccat taatgcgtca aatgattgca
    10141 caattttacc tggaacaaat gattcatatg gttcataaaa atcacgcgtc gtaatataat
    10201 cttctaaatc aaatgcatag aaaatcattg gctttttaaa tactgcatat tcatatatta
    10261 aagatgaata gtcactaatt aataaatctg ttatgaacag tatatcatta acttctctaa
    10321 agtcagaaac gtcaacaaaa tattgtttat gtttgtctgc aatattaagt ctatttttca
    10381 caaatggatg cattttaaat aatacaaccg cgttattttt ttcgcaatat cttgctaaac
    10441 gttcaaaatc aattttgaaa aatgggtaat gtgctgtacc atgaccacta cctctaaatg
    10501 ttggtgcgaa aagaatgact ttcttacctt taataattgg taattcatct tccatctctt
    10561 gtttgatctg tgtcgcataa gcttcatcaa atagtacatc agtacgtgga acacctgtag
    10621 gcactacatt tttctcttta ataccaaatg cttcagcgta gaatggaata tcggtttcag
    10681 atgatacata agcttttgta tagctacgat gatttaatga atcaataaat ggtccaccct
    10741 ttttaccagt acgactaaag ccaactgttt taaaggcacc aacggcatgc catacttgaa
    10801 taacttcttg agaacgtcta aaacgcactg tataaatcaa tgggtgaaag tcatcaacaa
    10861 agatgtagtc tgccttccca agtaaatatg gcaatctaaa cttgtcgatg atgccacgtc
    10921 tatctgtaat attcgcttta aaaacagtgt gaatatcata ctttttatct aaattttgac
    10981 gtaacatttc gttatagatg tattcaaagt ttccagacat cgttggtcta gagtctgatg
    11041 tgaacaacac cgtattccct tttttcaagt ggaaaaattt cgtcgtatta aatatcgctt
    11101 taaaaataaa ttgtcttgta ttaaatgatt gtttgcggaa atacttacgt aattctttat
    11161 atttacgaac gatataaata cttttaactt ccggagtcgt tacaacaaca tcaaggacaa
    11221 attcattaac atcgctagaa atttcaggtg taacagtata aaccgttttc ttcgaaatgc
    11281 cgccttttct aaattctttt aggtaagtct gcaataagaa attgatttta ccattttgtg
    11341 tttctaattc gttgtattct tcttcttgtt ctggctttag attttgatat gcatcattaa
    11401 tcacatctgg gtttaactgt gcaatataat caagttcttg ctcattcact aataagtact
    11461 tatcttcagg taagtaataa ccattatcta agatagctac attgaaacga caaacgaatt
    11521 gattcccatc tattttgaca tcattcgcct tcattgtacg tgtctcagtt aaatttctta
    11581 atacaaaatt actatcttct aaatctaggt tttcactatg tccttcaacg aataactgaa
    11641 cacgttccca atagatttta tctatatata tcttactttt aaccaacgtt aattcatcct
    11701 tttctattta cataatccat tttaatactg ttttacccca agatgtagac aggtctgctt
    11761 caaaagcttc tgtaagatca ttaattgttg caatttcaaa ttcttgacct tttaacaacg
    11821 ctaatttatc tacaatatct gggtattgaa tgtataagtc tacaacatct tggaaatctt
    11881 ttgaaccact tcgactacta ccaatcaacg ttagtccttt ttccaatact agacgtgtat
    11941 taacttctac tgggaactca cttacaccta acagtgcaat gcttccttct ggtgaaatgt
    12001 aatcgatcat ttgatttatg gctgattgac taccgcgacc acccacacac tcaaatgcat
    12061 gatcaaatgt taagccttca ggtattttat taataaagaa gacatcatca acaaatgaga
    12121 agtgactcaa tttataatct gttttaccaa agacatatat tttagactct ggatataatt
    12181 tacgtaataa aatggctgta atgtaaccta agttaccatc accccaaata ccaaatgtat
    12241 ttttatttga aatagatttc ttttcaaaac gacgaatagc atgcaaactt actgttacaa
    12301 gctctgtata tgaaataata cttaaatcaa tatcatcagg tagtggtaca gctctatcat
    12361 gatttagcaa cacaaaatct tgcataaacc catcatgtcc acttgatctg aagtagctcg
    12421 attttaaata gttttcagca atgacatcgt ctttttctgt cggcgtattc ggtaccataa
    12481 ctacttttgt acctttatta aacacacctt tactgtcaaa tacgacctca ccaacacctt
    12541 catgaattaa agacattggc aatttctgag ataagacatt ctcatcacgg ctaccagtat
    12601 aatatctttg atcggcagca caaattgaca tatataaagg acgtacaatg acatagtcac
    12661 tgtaaatatc tacgttgtta tacgtaactt caaattgtct aggtgcaact aattgatata
    12721 cttgattaat catcggcaat accacctcga ataatagcat tcgctacttt taaatcgtaa
    12781 ggtgttgtta ctttaatgtt atataactca ccttttacaa gtcgaaccgg tttgtttgtt
    12841 tctacaataa tcttacaagc atcagataaa atactctttt gctcatcact caactgtgca
    12901 tagctttctt ttaataaatt aatattaaac gattgaggtg tttgaccttg gtacatttca
    12961 ttacgcactg gaattgcatc aatcgtttga ttatctttag atgtaacaat cgtatctata
    13021 gcatcaatca ctgtatctac tgcaccgtac tctaaagcag cttgaatatt ttctttaata
    13081 atacgatgcg ttaaaaatgg tctaactgca tcatgtgtca caatgacatc gtcatcgtta
    13141 ataccatttg ttgattcaat atgtttaacg atattcataa ttgtatcgtt acgatcgcta
    13201 ccaccttgaa tgacttcaat tctttcatca gaaattttga attttctaag tgtatctttc
    13261 gtatgcgtca tccattgttg tggcgtcgcg ataataattt tttcaaaatc attaattaaa
    13321 ataaattttt ctaatgtatg gattaaaatc ggtttgttgt ctaaatctaa aaattgttta
    13381 ggtaaaggta cgttacccat tcttgagcct atacctccag ctagaatacc agcgtatttc
    13441 ataaaatact tcctccattc aactatatct atatttaatt atttaaattt cgttgcattt
    13501 tccaattgaa aactcatttt aaaatcaaaa ctctaaatgt ctgtgtatta cttaaaatta
    13561 tacatatttt gcttatattt tagcatattt tgtttaaacc tatattacat tatatcagac
    13621 gttttcatac acaaataata acatacaagc aaacatttcg tttattattt atatcactta
    13681 actaattaat ttataatttt ttattgtttt taagttatca cttaaaaatc gtttggcaaa
    13741 ttcgttgtga cgcttgtcca tcttctaatg aacagaattt ttgataaaat accgttcgtg
    13801 cttcaatata ctcatttgca gtctcatcga tttgttttaa tgcatcaatg agtgctgttt
    13861 gattttcaac aattggacct ggcaactctt ttttataatc catgtaaaaa cctctaagct
    13921 catcgccata tttatctaag tcatatgcat agaaaatttg cggacgcttt aatacaccga
    13981 agtcgaacat gacagatgag tagtcggtaa ctaacgcatc gctgattaag tataaatccg
    14041 aaatgtcttc ataatctgaa acgtctttca caaaatcatc atgttcatca atacgtgtca
    14101 caactaaata atgcatgcgt aataaaataa cataatcatc atccagcgct tgacgcaaag
    14161 cttctatatc aaagttaaca ttaaattgat atgaaccttc tcgaatcgct tcatcgtcac
    14221 gccaagttgg cgcgtacata atcacttttt tatctaatgg aatatttaat cttgtcttaa
    14281 taccattaat atattcagta tcattgcgtt tatgtgataa tttatcattt cttggataac
    14341 ctgtttccaa aatcttatct cgactaacat gaaatgcatt ttgaaatatc gatgtcgaat
    14401 atggattagg tgacactaga taatcccacc gttggctttc ttttttaaag ccatcttggt
    14461 aattttgagt atttgttcct agcattttaa cgttactaat atccaaacca atctttttta
    14521 atggcgtgcc atgccatgtt tgtaagtacg tcgttcgcgg tgatttatat aaccaatctg
    14581 gtgtacgtgt gttaatcatc cacgctttcg ctcttggcat cgctaaaaac catttcattg
    14641 aaaactttgt aacatatggt acattgtgct gttggaatat gtgttcatat ccttttttca
    14701 caccccatat taattgggca tcgctatgtt cagttaagta ttcatataat gctttggggt
    14761 tgtcgctgta ttgtttacca tgaaagcttt caaaataaat tagattcttg tttggcaatt
    14821 ttgatagtaa tttaaaagtc gtatatatac tatgttctat caatttttta attgtatttt
    14881 taatcatgtc gtacctccga cgtgtttttg taattatatt aatatgtatg agcaagctca
    14941 ttgtaaccat gcctattata gcatttcatc ataaaataca tttaaccatt acacttgtcg
    15001 ttaattatca tacgaaatac atgattaatg taccacttta acataacaaa aaatcgttat
    15061 ccattcataa cgtatgtgtt tacacattta tgaattagat aacgattgga tcgattattt
    15121 tattttacaa aatgacaatt cagttggaag gtgattgctt ttgattgaat cgccttatgc
    15181 atgaaaaatc aaaaggttat tctcattgta tagtcctgct tctcatcatg acatgttgct
    15241 cacttcattg tcagaaccct tcttgaaaac tatgccttat gactcatttg catggcaagt
    15301 aatatatgcc aacattagcg tctaaacaaa tctttgacta aacgttcact tgagcgacca
    15361 tcttgatatt taaaatgttt atctaagaat ggcacaactt tttcaacctc ataatcttca
    15421 ttgtccaaag catccattaa tgcatcaaag gactgtacaa ttttacctgg aacaaatgat
    15481 tcaaatggtt catagaaatc acgcgtcgta atgtaatctt ctaagtcaaa tgcatagaaa
    15541 atcatcggct ttttaaatac tgcatattca tatattaaag atgaataatc actaatcaac
    15601 aagtctgtaa caaagagaat atcgttaact tcacgatgat ctgacacatc gataaagtat
    15661 tgtctatgtt cacgtgaaat attaagtcta ttttttacga acggatgcat tttgaataac
    15721 acaactgcat tatgcttctc gcagtatctt gctaaacgtt caaaatcaat tttaaaaaat
    15781 ggataatgtg ccgtaccgtg accattacct ctaaatgtcg gtgcgaatag aataactttc
    15841 ttacctttta taattggcaa ttcatcttcc atctcttgtt taatttgtgt tgcataagct
    15901 tcatcaaata gtacatcagt acgtggtaca cctgttggta caacattttc ttctctaatt
    15961 ccaaatgctt cagcataaaa tggaatatcg gtttctgatg aaacatatgc tttcgtgtaa
    16021 ctacgatggt ttaatgaatc gataaacgga ccaccttttt tacctgtacg actaaagcca
    16081 actgttttaa aggcaccaac agcatgccac acttgaataa tttcttgtga tggtctaaag
    16141 cgaaccgtat aaattaatgg atggaaatca tcaacaaaaa tataatcggc cttaccaagt
    16201 aaatatggca atctaaactt gtctctccat ttgcgtctat ccgtaatatt ctccttaaat
    16261 accgttttaa tatcataatc aaaatctact ttttggcgta gtaactcatc atatacatac
    16321 ttgaaattcc ctgataaatt cggacgcgaa tctgatgtga ataatattgt tttgcttctt
    16381 ttaatatgta gtaactttgt aatattaaaa atagctttaa ataagaaact tctactttca
    16441 aatgaagctt tatgaccttg tttatgaagc cagtgtgcac ttgtcgcaat gacccctgat
    16501 ttctcttgag gtaaggtgat ttcaatatca aatacaaatt cgttaacgtc acttgctatt
    16561 tccggagtaa tggtatagac cgtatgttgt gatacgccac ctttacggaa aactttagca
    16621 tcatacgcta ataaatagtt cacttttcca ttttgtgtag tcagctcatt gtattcttca
    16681 atttgctctt catttaatgc cgcataagct tgatcaataa tatttggatt aagttgtcca
    16741 atatactcta actggtcatg ataaacaaat aaatatttat ccattggtaa ataataccca
    16801 tcgtctaata ttgcgacgtt gaatctacat ataaatgtat taccgtctat tttcatttca
    16861 tttgcttcta atgtttttgt ttcggttaaa ttcctcagaa caaattgtcc tgatgttaaa
    16921 tcgacacctt cactatgtcc ttcaatatat aactgaacac gttcccagta tatgttatca
    16981 atatgtattg cttgtttcgt ttttgtcatt gttattcccc catcggttta ttaaatcgtc
    17041 catttcaata ctgtttttcc ccaagatgtc gataaatcca tttcaaacgc ttggacgata
    17101 tcttgcatcg tacatacatt aatttcatgt ccttttaata atgctaactt ttcaactatg
    17161 tctgggtact tacgatataa atcaacaact tgctcaaaat ctttagagcc gcttcgacta
    17221 ctaccaatca acgttaatcc tttttcaagt actaatcgtg tattcacttc cacgggtaat
    17281 tcacttacgc ctaacaaagc aatactgcct tctggtgaaa tatgttcaac tatttgttga
    17341 agtgcaactt gacttccttt acctccaaca cattcaaatg catgatcaat tttaagatca
    17401 tctggtattt gatttactgt aaagatgtca tctacaaatg aaaaatgact taatttatag
    17461 tctgtcttac caaatacata agttttagct tctgggtaca acttacgtag caaaatagca
    17521 gtaatataac ctaagttacc atcaccccaa ataccaaagc tggttttcaa aggtatagat
    17581 ttacgttcaa atcgttgtat agcatgataa cttactgaca ctaactctgt gtatgaaatc
    17641 gtactcaaat caatgtcatt aggcagcgga acgatacgat catgtgccat cacaacgtag
    17701 tcttgcataa aaccatcata accactagat ctaaaataac tagaggctaa gtaattctcc
    17761 gcaataatat gatgttgctc tgtaggtgta ttcggtacca ttactacttt cgtacctttt
    17821 tcaaataccc ctttactatc aaatacaact tcaccaacag cttcatgaac taatgacatt
    17881 ggtaattttt tgcgtagtac attttcatct cttcgacctg tgtaatacct ttgatcagct
    17941 gcacaaatag acaagtataa aggtcttacg atgacatgat taccataaat atcaacatta
    18001 ttatatgtga cgtcgaactg tctcggtgca acgagttgat atacttgatt aatcatcggc
    18061 aatatcacct tgaataatgg catttgctac ttttaaatca tacggtgttg tcactttaat
    18121 gttgtatagt tctccacgta ccaatttaac tgcatgtcca gattcgacaa tgattttaca
    18181 tgcatctgat aagatttctt tttgttcact acttaaggcg cgataactat cttgtaataa
    18241 tttaatatta aatgattgtg gtgtttggcc ttgatacatt tcattcctta cagggatact
    18301 gtgtatgttc tgtttatctt tagacattac aatcgtatca attgcttcaa tgactgtatc
    18361 tactgcacca tattttgctg ctacttcaat gttctcttta ataatacgtt gagttaaaaa
    18421 tggtcttacg gcatcatgag ttacaatcac atcatcatta ttaattccat ttacattgcg
    18481 aatatggtcg ataatgttca taattgtttc atttcgatcc gtaccacctg caactacttt
    18541 gacacgttga tctgtaatgt tatatttttt taaaatatcc tgtgtatggg aaatccactg
    18601 tgctggcgtt gcgataataa tctcattaaa ttcactcact aaaatgaact tctcaattgt
    18661 atggattaaa atcggtttat tatcaatatc taaaaattgt tttggtaatg gcacgttccc
    18721 cattctcgaa ccaatacctc ctgctaaaat acctgcataa atcatgttgt cctccattct
    18781 gtcattacat catttccatt tatacattac tgacctatgc ccgcacataa gcctaaccta
    18841 ttgctcactt gcctctttta ttaatccaaa gatagttgtc acaatagtgt gataattttt
    18901 tataaaaatg tatttttgta actgaccatt ctaagttgtt ttgccatgca gttaatcatt
    18961 aactctgacg atattaaatt gttaaaggta ttaatgttta ctctttttca aattcattat
    19021 tactgccatc attttaccat atattataat aaatttatct tattaagtgg ctgtacttga
    19081 ttttcacttt aaaaattatc aaatattgcc atctcatttt aagtatacaa aatgcaaaac
    19141 aaccgattca caagcatatt tcacacaagt aaaccggcta tttatcaacg tatattcgaa
    19201 gatgaattat ttcgatagta tctatagacc agacggcatt cgcactttca tagctataac
    19261 tataccagcg ttttcgtcct caaaggtgca tactaataaa tcgtaaacat gactttatca
    19321 aatcgttctt tcttgttaac taatttatca aatgtctccg ggcctttttc taacggtaaa
    19381 aaatgagaaa taataggctt tacattaata tctttcgtct tcatataatg taaggttgcc
    19441 gtccactctt tgcccggaaa attactggac aaacagttcc aagagccaca tactgtcaac
    19501 tcgttacgca gaattttttc aaaatgaacg cgatcaatct caatatcatc atatggtatt
    19561 ccgagtaata ccacctcgcc acctttttta ggtagcgtca atatttgacc aatcgtaact
    19621 ttagcacctg atgattctat agctaaatcg atttgattgg cgtaatgatt ttcgatgaat
    19681 ttctcaagat tttcttcttt tgaattgatt gtttgatgtg cgcccaatga tgttgcaata
    19741 tctagtttat gcgcatctat atctatagcg atgatatgtg cagcaccaaa tattcgtgcc
    19801 cattgaatag ctaacaaacc tatactgcca caccccatta ctgcaacagt cataccaggt
    19861 tgtatattcg atttataaaa cccatgcgca acaacggctg atggctcaac cattgctgct
    19921 tcaatgtaat caacattgtc tggaaccttt aaaacatttt gcgctggcaa tttgacatat
    19981 tccgcgaacg atccaggttc atatgagcca atgacgaata acttttcaca tcgtgcatat
    20041 tcacctttta aacaatactc gcattgataa caaggtattg ctgggcaacc tgtcactttg
    20101 tcgcccacat taacatgcgt aacatcactt ccaatggcat ctactacacc tgaaaattca
    20161 tgaccaaatg gcataccttt aatgtatggc cccatttttt tgtatcgtga cgtgtctgaa
    20221 ccacatatgc cagtcgctcg tactttaata ataacgtcat tcgcactttc aatgactggc
    20281 ttttcattat cctcataccg taaatcttcc acgccatata atttcaatgc tttcacttgt
    20341 aaatcacctc aaatttgatt taattcacaa cttttttctt tttaaaaata cctgtcgcaa
    20401 aataacctgc aatgacaatg gaattactta cgagtaaatg ttccatataa aaatcagtga
    20461 tttgtcttaa tggcccaagc ataaaagtta gcattgattc taccatatta attctctcct
    20521 tcattttcat ctaaatcgag cggcttaagt aatactttaa taccttcgcc ggattttaaa
    20581 tgttgatacg cctcgtccca tttcgaaata tcatatattt ttgtcaccaa agcttcagca
    20641 tttactaaac catccgccat aagttgcaat gaaggttccc aatctgctgg cttttgactt
    20701 ctactaccaa caactgttat ttctttttga atcacttttt ccatatcaaa tggaatttca
    20761 gcatccttaa aaatacctat ttgactgtag aaaccttttt tgcgtaaaat atccaaacct
    20821 tgtcgtgctg ctggaactgc acctgaacat tcaacaacaa catctgcacc gtaaccgtct
    20881 gtaattccat tgatatacgt ttttaagtct gtttgttgta aattgactac ataatccatg
    20941 tgcaatgctt ctgctttatc taatctgact ttgtcattgt ccaatccagt taccacaaca
    21001 gttgcgcctt tactttttaa cacttgtgct acaagtaatc cgattggccc aggtcccatt
    21061 acaactgcta catcgcctga attgacttga atcttagaaa cgccatgata tgcacatgct
    21121 aatggttctg tcatagctgc agactgatac gatacttcgt ctggaatatg atgcaaactt
    21181 tcttcacgtg caatgacata attagtaaat gcgccatcaa cttgtgttcc aatacctttt
    21241 cgatggttgc ataaattata gtcttttgat ttacagtatt cacactcatt acaaacatag
    21301 aatgtcgttt cagatgtgac acggtcacca actttaaaat ctttaacgtc tgctccaact
    21361 tcaacgattt caccagaaaa ttcatgacct aatgtcactg gaaaattaac tttataatga
    21421 ccttcataag tatgaatatc tgtgccacaa attcctgcat aatgtacttt aatctttact
    21481 ttatcatcta gcggtgttgc aacttcttta tcaagaagtt ctaagttgcc atgtccttct
    21541 cttgttttta ctaaagcttt caccacaaac acctcgattt ttaattgaat agactaaata
    21601 gtttaaagat aagatagtta acgatattac caccttgatc aatacttgaa atttcagatg
    21661 aaccttttgg catttgtaca ttcgtacctt tcgccatatc tgtgaaaatg ggtgctacgt
    21721 ctgttgcaat atatagtgaa attgcaatca taatcgtacc cacaatgaca gaatgaataa
    21781 tgtttcctct tgctgcacca acaataaacg cgacaacaaa tggtatcgtt gctaagtcac
    21841 caaaaggtag tacttggttt cctggtaaaa taacggctaa taaaacagtg ataggtacta
    21901 aaattaatgc tgtcgaaata actgctggat gacctaatgc tacagccgca tccaatccaa
    21961 tataaatttc acgttcgcca aaacgtttat ttagccatgt tcttgcagac tctgaaactg
    22021 gcattaaacc ttccattaag atttttacca ttctaggcat taataccatt actgcagcca
    22081 ttgacattcc taaattaatg atgtctccag gtttgtaacc tgctaacaca ccaataccta
    22141 aacctaaaat caagccgaca aatatagact ctccaaatgc gccaaaacgt ttttgaattg
    22201 tttcaggatc agcatctaac ttattcagac cgggtacttt ttgtaacaat ttaactaagt
    22261 aaatacctgg tgcataagaa attgtacttc ctgtagcaat cgatacacct ggtaaatcaa
    22321 agaactcact catcatcggt gctgtccaat ctgctacttt caaacagata atttggaaaa
    22381 taactgctgc taataacgct tgccaaatac tgcctgatac ggcataaacc attgctgctg
    22441 taaacgtata atgccaaaaa ttccaaatat ctacattcat cgtctttgtc actttagtta
    22501 ctagcaatac aacgttaact atgattccga gtggaataat aaatgctgcg acagatgatg
    22561 cccaagcgat agatgatgtt gctggccaac ctacatcaat cacattcaga ctgacgccta
    22621 aatttttaac catcgcttgt gctgctggcc ctaaattttt aactaataaa tcgatgacta
    22681 agaaaatccc tacaaaagcc acacctattg ttaaaccaga cctaaatgcc gctccaattt
    22741 tctgcctaaa gaataggcca agcaagaata tgacaaccgg taaaataaca gttgcaccta
    22801 aatctaaaaa tccccttaca aaatcagtga agtaactcat atttaaaccc tccctgttat
    22861 atatgcattg tcacgatact ttccgattgt gattacattt gacgttacag tcatttcaac
    22921 gacaaccctt gctaaattcg actgcagtcc ttttgaatta cagtcactgc gtttctatgt
    22981 catcaacaat catttgtcgt gatagtcatt tatatgcaat ttgcatatat taatatgtta
    23041 tcgacccacg ttacatatca attccgttat ttttgtaact ctgttaagat ttgttgtttt
    23101 gtttcttcaa taccaatacc agttaagaaa ttacgtgcgt tgataactgg gaatttatat
    23161 tctttttttg tcattgcagt tgtaactaat aaatctgcag tgtcttcata aggtccaact
    23221 tctgtaattt tgatttgttt aatatctact ttaatattgt gttcctttgc catttcttca
    23281 attgcattat ttactactgt tgacgttgca atacctgcac cacacgctac taatacttgt
    23341 ttcattttca attcctccaa ttaattttta gttatattcc aaataatcat tgattagtgt
    23401 tgctaaaatt gtttcatctt tcgttcgtag aatctgctcc aatttttctt cactttgaaa
    23461 aatttgcatc aactgttgta acagcttaag ttgatcatct actttatcca ttgctaacat
    23521 aaaaacgatt ttcacttctg tctgttgatc aagtgttccc atttcaataa acggcacttc
    23581 tttttctaga acagccacac ctatcgttct atggttaata tgttcgacat ctgtatgcgg
    23641 tatagcgacc gaacatagat gcgttggtaa accagtagca aattcttttt ctctgtcgat
    23701 gactgcatct ttaaacgttg acttcacgaa cccattttga aataacacat ctgacatttg
    23761 tgacaatacg gattctttat cagttgccga caaattgagc attatatttt ctttatgcac
    23821 taattgctgt cccatccatt ttccctcgct tctttatttg aataattttt taaaatctca
    23881 tttacatcag aatttttgcg actttgtatg atgcgcttaa ttgcgtcatt gtcttgcgcc
    23941 acatctctca attgtagtaa cgctcttaag tgtgtcactt tatcaacagc agcaataggt
    24001 acaataatat ggattgctgt gccatctgac atgtatattg gttcttgtaa tatcaacata
    24061 ctcatcgctg ttttatgtac atgcttttca gagtatgcat gtggaatagc aatattttgc
    24121 atgataacca tatacgaatc atcaaatacc tgttgcatct catgaatata tttaatatca
    24181 ataaaatgat tagcaactaa cacatcactt gctttagcaa tagcttcatc aatattttca
    24241 acatgatgca ttcttttcac gtgccttgcc ggtatcaagt cagctaaatc taatgtctta
    24301 ttttgtgtga caatcgatcc attaatggtt gaaattgaat tataattggc aataaaatct
    24361 tctaaaccat cacgtagttc tgtaatgtca ttaactgtcg ttgtgcgttc aattaatgcc
    24421 attaacttgt ttatttcctt atcaatgtca gccgattcct tattaatgta cttcatcact
    24481 tctttacgta actttcgttg ctcattttca gataaagcta cttttgtgat aaataatttt
    24541 ttatgtgtta ggacaaacat tggtgaaaag acgatgtcat aatctaatgt gtaattttca
    24601 aatgttctaa gtgaaatcgc atctaagaaa ataatttctg gaaataagtt tcgcaactcg
    24661 tataacatca tttgtgatac tgacgtgcct tgtgtacaca cgataatagc ttttatcttg
    24721 ccatcgaagt tttcatcttg acgtctcaaa ctacctccga acaacatggt taaatatgct
    24781 atttcattat caggcaacga ttttccgaaa tattcagtta acgattgaca tgattgtttc
    24841 accatatgaa ataaggattg ataatttcct tgtaaaggat ttattaattc atcacgatcc
    24901 gttaagttat atttaatcct ataaaaagca ggcgttaaat gtaacaagag ttgctgtgat
    24961 aatttctcct tatcttcaat gttaataaaa gtgatttgtt caaaatggtg aatcatttga
    25021 gcgatggcca tcgttaaatt cgatatgcta tctgattctt gcaaatcagt ccattgcaca
    25081 cttgttgaaa gtaagtgtaa tgtcaaatat aacttttccg cttctggcaa atccggctca
    25141 tgttgcgtca taatctccgt tgcttgatat tctttcgtat ccctcaaata ctgataatta
    25201 atatttaatg gattcatcac atgaccactt tgaattcgtc tacgaatcac acaaaggaca
    25261 taaggcaatg aactaagtga tttgtctata aagcgactct tcaaaaattg ttctacctgt
    25321 ttgatcttgt ctttttgata tgcgatatct tcgaatgtta agttgagcgc ctttaaaact
    25381 tcacttttag taatatcatg attcaacctt tgatcaatca acttaatgaa gaaacggcga
    25441 acttcaaatt catcaccaac aatttcataa ccatgttttc gagaatactt aagtgacaaa
    25501 ccatgatttt ccaattgctc tttcacatga tttatatcgt gaatgacagt atttttactg
    25561 acttgtaaat caattgaaaa atggtttaga gacattgcgt tttccttact aaaaagcatg
    25621 agcattaaat aataacgacg tgtttctatg ctaaaaatga cattgttgcc gtttaacatt
    25681 tgctgctccg atacatctcg cttgaataac gtcatgattt cagaacttac aataaaattt
    25741 ccttggcttg ttctttcaag ttttggataa ccctcttgtt caagccacaa attgattttt
    25801 tgaatgcgat atcctagttg tctacgagac aaaccaaata tcgattcaag ttctttacca
    25861 tgaatagtag gattcaatac aatttctctg agtatttgct gattatcccc gttcatgttc
    25921 gattctcctt tgagcatctg agctcaattt atcatcaatc gtcacaccga tgtacacact
    25981 ttgaacacat attttcaaaa tgagcatgta catcattgtg atgttttaac aacatttcaa
    26041 ttatatctat attttttgtg attttaatct tttaaaataa agcaattgaa atttttgcat
    26101 atatttttgt gttttgtgtt tttttgaagc atttttaaca tacatatctc aatcattatc
    26161 aaattgtcat gaccattgta acccaataca aaaaccctaa ggacgcttat atcaggcgcc
    26221 ttagggttaa ctgtatctat ttaattaagt attattattc gtatgtacgt aacttatggt
    26281 ctatcaagtt ccacacttct tcaacatcaa ctgctgtagc aaaataagca ttggcaggct
    26341 tacctgtaac atgatttaaa tcgacagcca tagtgccata agttagtgga ctttgatgtt
    26401 caatgtcgat attaacgggt accattgtaa acaattctgg ttgtaacaaa tacaaaattg
    26461 tacaagcatc atgtattgga ccaccatcca tattaaagtg agtcttgtat gtcttcttaa
    26521 agaattgcaa taattctacg acgaactgtg caacaggatt attgatactt tcaaagcgtt
    26581 caatcacgtg atcgtcggct aaaacttgat gtgttacatc taaaccaaac acatttatag
    26641 taatcccact ttcaaaaaca cgcttcgctg cttcagcatc tacccaaata ttgaattctg
    26701 ctgtaggcgt ccaatttcca aatgtaccac cacccatcaa agtaatagat tcaatatgct
    26761 cagcgattct tggctcacga atcaatgccg ttgctacatt cgtaagagga cctgtcgcta
    26821 caattgttac aggtgtatca ctcgtcatca ctttgtttat aatcacatct gatgctggca
    26881 ttgcaactgc ttgacgtgat ggtgtcgacg gtagtttcgg accatctaat ccagattccc
    26941 catgtatttc agaagcaaag gcagctggtt taattaacgg cctatccgca cctttcgcta
    27001 ctgctatatc ttggcgtccc ataatatcca atacgttcaa ggcgtttgtc gtattcttgt
    27061 caactgattg attacctgcg actgttgtta cagctaatat ctctagtgga ctgtcaattg
    27121 cccccgctaa aattaatgct attgcatcat cgtgtcctgg atcacaatcc ataataatct
    27181 ttcttttcat ttatatatcc acctttctta agttgttatc gatagcttat gtatatttat
    27241 ttatgtggtg aatcatgttt attttgaaaa atagttttaa ctttctcata tttttggata
    27301 caaacactat ttatctattt tatggcttat aaatttatcc gatatgcctt atcaacctac
    27361 ctcgctaaaa ataggatgtc tacatatcta taccgacttt tgtcaactca ttttcacaac
    27421 aatataaaca gcaatttata tgattgttac atgattcaaa caatttttat gaaaaatatt
    27481 ttcatacaca gaatatatat tgatattaaa tttctcaaaa gctatattga gaataattag
    27541 gagggatgtt gatgaaatct ttatttgaaa aagcacagca gttcggcaag tcctttatgt
    27601 tacctatcgc aatcttacca gctgcaggtc tattgttggg tatcggtggt gcattaagta
    27661 atccaaacac cgttaaagca taccctattt tagatattac cttattacaa aatattttta
    27721 cattaatgtc agctgcaggt agtattgttt tccaaaattt accggtcatc tttgcaattg
    27781 gtgtcgcaat cggattatct agaagcgata aaggtactgc aggtttagct gcgctgctcg
    27841 gtttcttaat tatgaacgca actatgaatg gcttattaac tatcacgggc acattggcaa
    27901 aagatcagct tgcacaaaat ggacaaggca tggtgctcgg tatacaaacg gttgaaaccg
    27961 gtgtttttgg cgggattatc acaggtatta tgaccgcaat acttcacaac aaatatcaca
    28021 aagtggtatt accaccgtat ttaggtttct ttggtggctc tagatttgtc cctattgtca
    28081 cagcatttgc cgcaatcttt ttaggtgtat tgatgttttt catttggcca agcatacaag
    28141 ccggcattta tcatgttggt ggatttgtaa cgaaaacagg tgccatcggt acttttgttt
    28201 atggcttcat cttaagattg ttaggtccac tcggtttaca ccatattttt tacttaccgt
    28261 tttggcagac ggcacttggt ggtactttag aagtcaaagg gcacttagtt caaggtacgc
    28321 agaacatctt ctttgctcaa cttggtgatc cagatgtgac gaagtattat tcaggtgtgt
    28381 cacgctttat gtcaggccgt tttattacga tgatgttcgg cttatgtggt gccgcacttg
    28441 caatttatca cacagctaaa cctgaacata aaaaagttgt cggcggttta atgttatccg
    28501 ctgcactcac ttcattttta acaggtatta ccgaaccttt agagtttagt ttcttgtttg
    28561 tcgcacctat tctttatgta atccatgcct tctttgatgg attagcattt atgatggcag
    28621 acattttcaa cattacaatt ggtcaaacct tcagtggagg ctttatcgat ttcttactct
    28681 ttggtgtgct acaaggtaat agtaaaacaa actacctata cgtcatacct attggaattg
    28741 tgtggttctg tttgtattac atcgttttca gattcttaat tacgaaattt aatttcaaaa
    28801 cacctggtcg agaagataaa gctgcagcac aacaagttga ggctactgaa agagcacaaa
    28861 ctattgttgc tggtttggga ggcaaagata acattgaaat cgttgactgt tgtgcaacga
    28921 gactacgcgt cacacttcat caaaatgaca aagtcgataa agtattactc gaaagtactg
    28981 gtgccaaagg tgtaatccag caaggcactg gtgtgcaagt aatttatggg cctcacgtta
    29041 cagttatcaa aaatgaaatt gaagaattgc tcggggatta agactaaccg aaatatcaac
    29101 agaactaatg gcaacgatgt acgaagtaag aagtgacatc gttgctttta tttttaatgt
    29161 tacatttgaa gcattaagtt catcatgcac tgtagtgagc ccgcaaatcg cctctgctag
    29221 acaatcatct taatgctatg attaaagctt aagtgccaga tttgaattta atttcaacaa
    29281 cgactttcac tacattaaaa atagggctac tcgacacata tagttgtatc aaatagccct
    29341 ttatacaatt ttttgggtaa ggttttacaa tttttgggat ggtatagatt ttataaaaag
    29401 ttatttaagt tcttctgctt cagccataat atcttttaat gttttagctg aatgtgcgaa
    29461 cttgctttgt tcttcgtcgt ttaatgggat ttctacgacg ttgcgaatac cgtttctatt
    29521 gatgactgct ggaacaccaa tataaacatc ttcttcctca tattcgcctt ctaataatgc
    29581 tgatacagtc aatacggcat cttcatttct gaaaatcgct tcagtaattc tagctaatcc
    29641 cattgcaaca ccataataag tggcaccttt agcttgaata atgtcatatg ctgcatcacg
    29701 tgtttgaaca aaaatttgtt caatttgcgc tttgccctca ggacgttgtt caagtaatgt
    29761 cttcaaaggt tgacccgcaa tattagcgtg tgaccatact ggtaattcag tgtcaccatg
    29821 ttcaccaata atttgagcat cgacgctacg tggcgcaaca tcgaacgctt cgcttaacaa
    29881 taatctaaag cgtgcagagt ctaaaattgt accagaacct ataacacgtt ctttaggtaa
    29941 accagagaat ttccatgttg catacgctaa aatatcaaca ggatttgtag ctaccaagaa
    30001 aataccatca aattttgatg ccattacttc accaacaatt gatttgaata ttttcaagtt
    30061 tttagatact aaatctaaac gtgtttctcc aggtttttgt gcagcaccag cacagatgac
    30121 aactagatcc gcatcatgac aatcactgta ttcgccagct ttcacacgaa ctgttgttgg
    30181 agaatatggt gtggcatgtt ttaaatccat aacatctcct cgaacttttt cagtgtctaa
    30241 atcaatgatg actaattcat caacaatgct ttggttcact aatgaaaatg cgtagcttga
    30301 acctactgca ccattaccta ttaatacaac tttgttccct ttaaatttgt tcattacaaa
    30361 aactccctta tgattaattc actaacatac atgtagcttc aaatatgtta gtttaatgct
    30421 gcttattgac gatacaaaag caaataaaca tctcttttat tttcaacgca taacttaaaa
    30481 ggtcatgtgt catccgcttt taagtttgtg atttatttca catataaaat gtaacatgca
    30541 ttaagtactg ggtcaatatt aaattgtgat ttatttcaca ttttatttta atttttacac
    30601 ctttttaatt tgtatacgat tacatcttag atgtctttag tcttcgtact tcgccagtga
    30661 ttatttacac tttcacattt ttattatcat gtttactttt ttctaggaaa acaacaatgt
    30721 tttttgaatt agtcaaataa atgcgctcaa tcgtcggtgt gcaaacagac aattgtacac
    30781 aatgcttatt gataagtatt taaaaaatta aaaatgtcat acaattatca aatttgccat
    30841 tttatttata ttttctcaaa ccaattaatt gaatatcgaa atttttagta gaataatcaa
    30901 aatatacaga ttaaaggagg agtatcatgc ttacagaaca agagaaagac attatcaaac
    30961 aaacggtgcc tttacttaaa gagaaaggga cagaaattac gtcaatcttt tatccaaaaa
    31021 tgtttaaagc gcatcctgaa cttttaaaca tgtttaatca aacgaaccaa aaacgaggca
    31081 tgcaatcttc agcattagca caagctgtaa tggccgcagc ggttaatatc gataacttaa
    31141 gtgttattaa accagtcatt atgccagtcg catataaaca ctgcgcacta caagtttatg
    31201 ctgaacatta tccaattgtg gggaaaaatt tattaaaagc cattcaagac gtgacaggat
    31261 tagaagaaaa tgaccctgtc attcaagctt gggcaaaagc atatggcgtt attgcggatg
    31321 tgttcatcca aattgaaaaa gaaatttatg atcaaatgat gtggattggt tttaaaccat
    31381 tcaaaattac caatattaaa caagaatctg aagacattaa atcatttaca gttgaaactg
    31441 aagaatatga ctttagtgaa tttacaccag gccaatacat cacagttgat gtttctagtg
    31501 ataaacttcc atatagagct aaacgtcact attctatcgt atcaggtgaa aaaaaccatt
    31561 taacttttgg cgttaaacgt gatgtcacaa cagaacatga aggcgaagtt tcaacaattt
    31621 tacacgatga gattaaagaa ggcgatatga ttaatttagc tgcgcctgta ggtggattcg
    31681 tattagagaa tacgactgaa ccacaacttt tcttaggttc aggtattggt gttacacctt
    31741 tagtagctat gtatgaagct gcctctgcca aaggtttaga tacacagatg gttcaagttg
    31801 ctgaaaatga acaacattta cctttcaaag acaacttcaa cagtatcgca agccatcatg
    31861 acaacgctaa attatataca cacttaaaag ataaacaagg ctatattggt gctgaagaat
    31921 tacaagtatt tttagcaaat aaacctgaaa tttatatctg tggtggtaca aaattcttac
    31981 aatctatgat cgaagcactt aaatctttaa attacgatat ggatcgcgta cactacgaaa
    32041 catttattcc aagactaagc gttgcagtat aactgagaaa agaggtttag tgctcatatg
    32101 aaacttaatc aacgttacgt aaaagtattt gcattatatt tcgtaagtat tgttactgca
    32161 aatattattg ttaaaaataa taatttaatt aaaacattga tacaaaccat agccgggtac
    32221 acggtctttg cagttggttt gaagtattta actaaacgta aaaataaatg acatctatac
    32281 caaaacagct atgactttac tttgtcatgg ctgtttttta attgggagta ggacagagat
    32341 gatattttcg caaaatttat ttggtcgtcc caccacaaca tgcattgatg tatgctcact
    32401 gaatttcata agaaaggagt tcacaagatg accgtagata ttggacggat ttatgacaat
    32461 aaagataata ccgacgctat tcgtatccta gtcgatagag tctggccgag aggtatttcg
    32521 aaaagaactg ctaacctaga ttattggtta aaagacattg ccccttctac tgagttgcga
    32581 caatggttcc aacatgatcc taaacttttt ggagctttta aagaaaaata tgaaaaagaa
    32641 ttacgtgatc aggatgcgca aaaagatgct tttgaaaaat taaaggatat tgtaaatcag
    32701 cataatcatg ttctattgtt atatgcagca aaagatacta aacataacca agctgtagta
    32761 ctacagcagt tgctcaatac ttagttatat atgtttatcg tgaatcacta taaatgtcgc
    32821 taacttcatt tctaatattt atttttaaaa agcataatta ctacaattaa ttgaacttta
    32881 ataattacta aacttgaaca acattttact ttaacaaaat aaagttttaa aatattaatt
    32941 gttgggtttt acatagtaat tagaaagaga gtgttaatga aatgaagttt aaaagactag
    33001 caactatatt ttcagcagta ttagtgttat caggatgtgg ctcaatgcat tcatctggca
    33061 aagacttaaa tatttcgtta ccgttaaaaa caaagtctat tgcaccttat gaaacagatg
    33121 taccagttaa gattggtgct gcagaatcat tatttaaaac aaatgatcaa ggaaaaatag
    33181 aaaaagcatt agtgaaatcg tatcatcaac caaatgatac aacgttagat atagaattaa
    33241 aagataatat taaatttcaa aacggtcaaa aattgactgc agaaaaagtg aaatctagcc
    33301 ttgaaaatag catgaaaaaa agcgacttgg tcaaatattc attaccaata tcatcaatta
    33361 ccgctaaagg tcaaaaactg acaattaaaa ccaactccgc ttaccctgaa cttgtatctg
    33421 aattagctaa tccttttatg gcaatttatg atacagatgc taaatcagat gttaatcaaa
    33481 ctcctgttgg tacaggccct taccaaataa aagattataa gcaatctcga aaaatatcat
    33541 tgtcgaattt taaggactat tggcaaggta aaccgaaact tgatcatatt actgtgacgt
    33601 accaagaaga cggcaataat cgcgtcagaa atttagaatc tcaaaaagat gatttaataa
    33661 ctgatgtccc agttaataaa gttcaagaca tagaaaataa tcaaaattta aaagtgtcaa
    33721 aagaatctgg atttagaact tctttactta tgtataatca tactaataaa aaaatgacta
    33781 aatccgttcg tgaagcatta gatcatatca ttgatagaca aggtattgca gatcatattt
    33841 atcaaggtta tgcgaaacct gcaacgagtc catttaatga taaaattcca tatattaaag
    33901 aacctaagtt aaccaaacaa aatatcgaac aagcaaaaat gttattagct aaagatggtt
    33961 atacaaaaga acacccttta aaaattaagt taattacgta tgacggtcgt ccagagctat
    34021 cgaaaattgc gcaagtatta caatcagatg ctaaaaaagc aaatattgaa atcgacatta
    34081 aaagtgttga tgacatagaa ggttacttaa aagaccgttc tgcatgggat gcaacgatgt
    34141 atagtttcgg aacaattcct cgtggagata caggttattt ctttaatcaa gcatataaaa
    34201 aagatggtgc tattaataaa ggagactaca ataatagcaa cgtcgatgat ttaattaatc
    34261 aattgaatca tactgtagat gttaaggaac gtcacaatat ttctaatgat attattaaat
    34321 tatcaagtcg agatgtacca aacagttata ttgcttacaa cgatcaaata gtcgctgcta
    34381 actctaaggt taaaaattat aaagtaacac ctgaaggtat ctatttaatc gattatcgaa
    34441 caacgattga acgataaggg agtgtgggac attgatttat gtctcagccc tgcttcaact
    34501 ataaaaacca cgccgaagtc atccaatatg attatggcgt ggtttaattt gcttatatga
    34561 ttgtattaat ttctgtttga tgaatttcaa taaaatgatt attacatact ttcagtgaat
    34621 catgttcatt ttatataaat ctaattgtca ctatttgttt aacgtgaaac acttttttaa
    34681 ctccaaaaag tattcctatt ccactctttt aaagacaaat tataaaaata gcttatgtca
    34741 caaacatatg agcaacataa gctttaatta ttattcgtct acttttttct gttgttcttt
    34801 aaattctttc tgcagtaaat tcacttgttt taaacctgcc cccattaaaa tgaatacaag
    34861 tatccacaca ataacgatta aaataaatat tttcaacgta tcactgccaa gtacagttaa
    34921 agatgtatcc caaatgccat gtaaaacaac ggctgataaa aagaatatta aaaagcgttt
    34981 atcaaaaata tctttgaatt caaagccatg ctgttctttc gcaataacta ttgcagcacc
    35041 aacaatcgct gaccaaacta aatgaccacc aatcgcagtc cacgcacgtg tgaagacaat
    35101 atctaataat gggacatttt ctcctaaagc gaaattcaaa atataacctg ctgattcaaa
    35161 aactgcgaac cctgcaccaa tagcagcacc gattaataat ccattcaaaa tcttatttgt
    35221 tttcaattta ttgacgaaat aaacaataat gagtgctttt ccagtttctt caactaatcc
    35281 tactaaaaat gcatcgaaaa atgttaaaga accaaacctt tcgaattgat cactaaaaac
    35341 gacaaatcta tataatacca tcgtacttag taatgaaaat acgccgccaa taaagaacat
    35401 gataataact tcaaaaatgc taatattttt aaacgcattt gattcataaa agaagaacaa
    35461 acccgataat ggtactgtta aagcccctat aaaaatgaga cccggaatcg cgttagtgtt
    35521 attaaaaatt gctgccatga cccataatcc aataaatgtt actgtgaaag cgatgaatac
    35581 tcgagaaaag agccatggct tcccccattc ttctgatatg tcacaaattg ctggcgtagt
    35641 tttagcagta cccgctataa atatttcatc tgcttcgttt ttagtatgcg gcttaaatac
    35701 ttcagaaagc atatctttta aattaagatt taagctttca tcattgcccg ctagtctacc
    35761 gattgatttg gttgcattat caaataaact tcttgcttcg ttttttaaat ctgtattttg
    35821 cgctgtttca tgcgctttag cttccatttg tttatcgata gattcattat gtatgtcgga
    35881 cgtcttagtt gattgtgtac ttgctgatga catcccctct tcacctgtat taggtgcaac
    35941 tgattgcgtt tgtgttgtct cttctttttc tgtatttctt cgatgattaa gaccttccat
    36001 agacttacct actttcactt ttcataataa aactatttta atttaatatt gatggatttt
    36061 ccatatttta atataaatac tttgttcgat tgggcacttt gtatttcata tttaaaaatc
    36121 aaaacctatt taacttttta tattccgaac aatattttta tataagtatt ttaatgatat
    36181 tcaattcatt acaaaccctt gatattacaa ttgttatcga ccgtcaaaat gtgtctatac
    36241 tgtgtctatt atcagaataa aaagatattt ctaattgatt ttaaacgtcg ttatgttata
    36301 tttcttgtaa gggtttacac aaagtgtaaa aacgctatat tttatcaaat gtgatggagg
    36361 tgacttgctt gaaacaaatc acatggcacg acttacaaca tatcattaaa gatggtgatg
    36421 tgattggttt accagcatta gctgtagcca acttacccgc cgaagttcta cgtgctgtgt
    36481 tagcgcaaca tgacacatat catacgccca atgatttaac gtttatatta gcgaatgata
    36541 ttcatagttt aggtgccgca ccggatttag atgattttat agaacgtcgc atgattaaac
    36601 gtgtcattat gagtatttta acggcttctt ccaaaacggc acaagcaatg aaaaataatg
    36661 acattgaagc ttatttttta ccacaaggta tcattgcaac tcattatcgt cagagtaatc
    36721 aattattacc tggagttatt actaaaatcg gattaaacac agctgttgat cctagatacg
    36781 gtggcggtaa agtaaataca cgaacaacgg atgatttagt ttcattagta accatcaacg
    36841 atgaaacata cttacattac acattcccta gcgttgatgt ggcactactg agaggaacat
    36901 acgcagatca acaaggtaac atttatttaa ctcaagaagc gtacttgagc gagtgttatc
    36961 atgtcgcatt aaacgcgaaa gccaatcatg ggaaagttat tgtacaagtt aaagctttag
    37021 ttgacgacta tcaactaaaa ccgaatgaag ttgttatccc aggaaatctt gtcgattatg
    37081 tatacgtcac agaagatgaa aagaatcacc gccaagtaat tcaaagtcat tatttaccag
    37141 ccttgtctgg agaagaacga attgatggaa tacctgaacc cgcattacct tttaatagtc
    37201 gcaaattgat tctccgacgt gctgctcagt ttttaactta tggcgataca attagcatcg
    37261 gttatggcat caataatgaa ctctctaatt tattgcatga agaatgtgtt gaacatgatg
    37321 tgcaaccgat tttagatgtt ggcattttcg gtggattcgt tgggagtcgt gaacattttg
    37381 gtatgaatta caatgcagat gtgcgcatgc ctcatgatcg agcatgggat tttatttata
    37441 acaatggtgt atcagttgcc tatcttagct ttgctgaggt tgatcaatac ggcaatgtca
    37501 acgtgtctta cttcaatgac cgactaaatg gatgtggtgg ctttatagac attacgcaat
    37561 ctgtaaataa aattatcttt tcaggtactt ttgtagctgg cagtcatgtc tcatgccata
    37621 atcaacgatt aaacattgaa actgaaggac aaaaccagaa atttgtatca gatgtgagcc
    37681 atatcgactt taatgcacaa tattcacaat cactcgagca agaaatctat tttgttactg
    37741 atcgtgcagt attcgaactc accaatcaag gcttgaaact aattgaaatt gcaccaggtc
    37801 ttgatttgca taaagatatt ttgaatcaaa tggcttttaa accaattatt gctgatcatt
    37861 taaaattaat tgataccagc atttacaaag aaaaatgggg acaacttaaa caatcaattc
    37921 ataaagtatg aaagtgaagc taaagggggt atactaatga atttcgattg gataaaaacg
    37981 cgttcagact tcgatgatga caagcctgcc gttattgatc acgcaaaaca aacatcttgg
    38041 acataccaac aactcaatgc acgcgctgat aatatggcac attatttaac atctcaaggt
    38101 gttaaaaaag gcgatgttat cggtattttt gcgccaaatg atattgcaat attagattta
    38161 ttgtttgctt gttttaaaac aggtgcggtt tttttaccat tgaattggcg gcttaaccca
    38221 aaagaaattg cagccattgt ggaagatgca caattaaaac tgctcttcta tgctgaaaaa
    38281 catttaagtt cactcaccga tattgaccaa aacttattgc atatggatat tgatgtggcg
    38341 caatatgatg aaatcgtaaa tccagattat caccaacctt ttcaagcaac acctgttgaa
    38401 ccacaagatc ttgcagcatt aatttataca agtggtacaa ccggatcacc taaaggtgtg
    38461 atgttctcat atgaatcatt tgttcataac ggtgcaaact tagagctgac gtataagttc
    38521 aattcaaact atattacgat tgtatcaaca ccaatgttcc atgttttagg gtttaacgat
    38581 actgtattac cagtattaat gtcaggtggc acacttattc ttcaacgtta ctttaacggc
    38641 gaagaattga atgacatgat tgcacaatat caccctacat ttattatcat gattccgaca
    38701 atgtattata gtacgctacg tgctagtaat tttaatcctg aaaattttag agctatggat
    38761 tatatcatcc aaggtggttc acaaccatta ccaagtatcc aagcagcttt taaacaatat
    38821 ggcataaaca ttattaacgg ctatggttta actgaagcac ctcttgtact tgttaataca
    38881 ccggaaaatt caaaacgtaa gccaatgagt attggtaaag cagtcatgtt cgttgatgca
    38941 cgtatccttg atgataacgg tgaggaagta cctactggtg agattggcga acttgcaatt
    39001 aaggctaaaa atgtcacgcc aggatattgg aataaaccag cagagactgc caaagcattt
    39061 catggtcgat atttattaac tggtgactta gcgaagatgg acaacgatgg cgatatattt
    39121 attattgacc gcaaaaaaga attaatcata actggtggcg aaaatgtctt accatccgaa
    39181 gtcgaaaatg ctttagctga gcatccacta gtagaccggt gtgtggtcgt tggctatgat
    39241 catccaaaat atggtgaatc aattgctgca gccattatac ttcgcgaaga tgaacctcat
    39301 tacgctgaaa ttttaaatca acatatgcga agtcgtttag caggttataa agtcccaaga
    39361 atgtatgtac cagtgacaca tatgccgtta aacagtacgc agaaaccaga taaacttgcg
    39421 attcgacaaa tgatgaatga caaagtctcg caaacacttt aaaggtgata aaaatttttg
    39481 acatttagtg taagcgttta caaataaagc gtgttgtttt tgaattaaat gcatttcaca
    39541 ttagtattca tattattttt aggaggaatt tatatgacat ttgaaaaaga aacggtctta
    39601 aaaacattat ttcctgaaga tgtacttagt attgctaaag gtttaacaga cggtgaagtc
    39661 gaatttttac aacaagtaga ttcattgcta gaaagtaagt accgtgaaaa tattaatcaa
    39721 cattggatag acgctactgt acccgaggac tattttaaag atctgggaga attaaattat
    39781 tttaacaatc cattacttta caaggatcgt ccaaacgcca aaatgcctag tcaactattt
    39841 cagtttttca tgtcttacct actcgcgcga tttgatattt ccttagctac cctactcggt
    39901 gttcaccaag gtttagggca taacactttc tatttcggag gtagcaaaga acaaattgcg
    39961 aaatatgtac ctaaattaca gtcacatgaa ctgcgtacat gctttgcttt aactgaacca
    40021 gaacacggtt cggacgttgc gggaggtctt gaaacagtcg ctgaacgcca aggcgatact
    40081 tgggttatca atggtgaaaa gaaatggatt ggtggtgcac atgtatctga tgtcattcca
    40141 gtattcgcag taaataaaga aactggcaaa ccccattgct ttgtagtcag accagaacaa
    40201 gatggcgtcg atattgaagt cattgataat aaaatcgcac ttcgcgttgt tcctaacgcc
    40261 ctaattaaat taactaatgt caaagtagat gaagcggatc gcttacaaaa cataacaagc
    40321 tttaaagata ttgccaaaat tctttattca acgagagcag gcgttgctta tatggctaca
    40381 ggtggtatgg ctggcgcttt acgtgccaca ttagattatg tcactgagcg taagcaattc
    40441 ggcaaaccaa ttagtaaata tcagttaata caagaaaagc tagcaatgat gcaaggtaat
    40501 ttagctcaag caatggcaac atgtgctcaa ttagctaata tgcaagcaca tggtgaatat
    40561 gacgaggttg caacttcaac ggcgaagatg atgaatgcct tacgtttgcg tgagacagta
    40621 gctatgggcc gcggtattac aggtggtaat ggcatactag ctgacgatta tgatattgca
    40681 cgtttcttct ctgatgcaga agcgatttac acgtacgaag gtacacatga aattaatgcc
    40741 ttagtaattg gacgcgcttt gactggagat tctgctttcg tataaatagc aaataattat
    40801 atgagatgca ttaatttcac taaaaaagac ttattttaag cataaagctt tttccttaaa
    40861 taagaggcta agatgactgt caaagatact taattaattt tataaaatag caacgttatt
    40921 ccaattatct taatggttat cttatcctca actaaattgg aggaatcact atgacaatta
    40981 ataaagtaac cgttcttggc gcaggcacaa tgggcgctca actggcagca ctttttgtga
    41041 atgctggact taaagtaaaa ctattagata ttgtagtgga caaaaacgat ccaaatctca
    41101 ttgcgaaaaa atcttacgat aaaattacag ataagaaacg gccgctacta ttcgacttaa
    41161 atctagcgag tcatttaaca tatggtaatt ttgatgatga cttggtaaat gatgatgctg
    41221 atttatatat cgaagcagtc aaagaagata ttgaaattaa gcatgctgtt tggcaacaag
    41281 ttctacaaca tgctaaagaa gatgctttat tcgctacaaa tacatcaggt attccaatta
    41341 atgcgattgc tcaagcattt aacgagaagg atcaagaacg attctttggt ctacatttct
    41401 ttaacccacc acgtattatg aaattagtgg agttaatacc tacgtcacac acgaaggaat
    41461 ctattatatt agatgtaaaa aatttcgcgc aaaatgtgtt aggtaaaggt gtcattgtcg
    41521 tcaatgatgt gcctggcttt gtcgcaaata gagtcggcac gcaaacaatg aatgatatta
    41581 tgtatcgcgc cgagcaacac aagataagca ttgtagatgt ggatgcttta actgggcaag
    41641 cgattggtcg tcctaaaaca ggtacatatg cgctatctga cctagtcggt ttagatattg
    41701 cagtgtctgt aattaaaggc atgcaacaag tacctgaaga aacaccttat tttcatgatg
    41761 tcaaaattgt aaatacgttg tttgacaatg gcgcactcgg acgtaaaacg aaacaaggat
    41821 tttacaaaaa ggataaagaa actaaagctc gacttgttta cgatgttgaa aaacaagatt
    41881 atgtacctgt atcgcaacca caattaccaa ttttaaatga atttaataaa gacttagtgc
    41941 ataaccttga taccatattc aatgcgcaag acgaagcggg actattttta tgggagacat
    42001 tacgtaataa tttctattac tctgctatca atgtacctaa agctaccgat gatttccgag
    42061 acatagaccg tgcgcttgtc tgggggttca actggaaact tggtccattc caattatggg
    42121 atgcaatggg atacgaacgt gttaaaacac gtatggaaga cgaacttgga gacttaccac
    42181 aatggattag tgatttagat ggtggctttt ataaacaaga tgagaccatt gaatatgcaa
    42241 cacctatttc tcacttcgta aaagatgaac tttgggataa aggtgatgcc aaactttccg
    42301 taactcatga tgatcaactg ttactgaaat tacaaagtaa aaataatgtc attaccgatg
    42361 aattcaacga tgcgttagtt gatgcgattg atttactgga aaatgaccat tacacaagta
    42421 tggttattta tgcagatggt aacaatttca gtgtgggtgc taaccttttc ttaatgaaaa
    42481 aggcgcatga agacggtctt gtagatgatg tcgttgcaca atcaattgat aaattacatt
    42541 atagctttaa tcgtttgaag tatagtttga aaccagtagt cacagctgtt caaggtcgtg
    42601 ccttaggcgg tggctgtgag cttgtacttt actcacctat tgttgtcgct gcaagtgaaa
    42661 catatatcgg tcttgttgaa gcaggtgttg gcttattacc gagtggcggt ggccttgcag
    42721 aaatggctga tcgcatatta cgcacatcgc ataagtttga tgacaaacaa gcttccatga
    42781 caaaagtact gacgaatatc gcatttgcga aagtctctac aaatgccttt gaggcacgtc
    42841 gttatggtta tttacgtgat acagatacga ttattttcaa tacagcacaa cgtgtcgaag
    42901 ttgcgctcaa acgtgcgaaa tatgaagcag aaacaaacta tattccgaat cctagacatc
    42961 aatatatcgc tttaggtgaa gacttcaaag cattgatcca aggacaatta gatgcgcaaa
    43021 gacggggtca ttttattagc gaccatgatt atcatattgc cttaaatatc gccacaattt
    43081 tagcgggtgg tgatttacca agaaatacat ttatcaatca acgttacatt caatcgttgg
    43141 agaaaattgg ctttattgac ttactaaaat ctaaaaaatc atatgaaaga attgcacata
    43201 tgttaaaaac tggtaagcca ttacgtaatt aaaagatagt cattaagaga ggatgataac
    43261 catgcaagaa gcatacattg tagcttatgg gcgttcagcc gcagcgaaag caaagcaagg
    43321 cgcattattc cacgaaagac ctgatgatgt cgcagccaaa gtattacaag gcgtattgaa
    43381 acgtattgac ggaaaattca ataagaatat gattgaagat gtcattgttg gtacggcttt
    43441 tccagaagga ttacaaggcc aaaacattgc acgaacgatt gcattgcgtg cgggattatc
    43501 tgacacggta ccgggtcaaa cagtgaatcg ctactgctca tcaggattac aaaccatcgc
    43561 gattgcagcc aatcaaatta tggctggtca aggagatata cttgtagctg gtggcgttga
    43621 attgatgagt gccgtaccaa tgggtggcaa cgagcccaca aacaatccaa ccttacaata
    43681 tgatgatata ggtgcgtcat atcctatggg tttaactgct gaaaatgtag catcccaatt
    43741 tgacgtatca cgtgaagatc aagatgctta tgctgtcaga agtcatcaac gtgcctatga
    43801 cgcacaacgt gatggtcggt tcaaagatga aattattcca atacaagtaa actcagttga
    43861 atatacaaac gcaggaccaa aagtacacac aaatatcttt gaccaagatg aatttatacg
    43921 ccctgacacc acgatggagg cattagccaa attacgtaca gtatttaaag ctgacggcac
    43981 tgtgactgca ggaacatctg ccccactttc tgatggtgca ggatttgtag ttttaatgtc
    44041 tggagataaa gtgaaagaac tcggcgtgac acctattgca cgattcgttg gttttaaggc
    44101 agtaggcgtt gacccgaaaa ttatgggtat tgggcctgca tatgcgattc ctgaagtatt
    44161 gtcactcagc aatctatctg ttgaagacat tgatttgatc gaattgaacg aagcatttgc
    44221 ttctcaaacg attgcatcta ttaaagaagt aggtctagat atatcacgta cgaatgtgaa
    44281 tggtggcgct attgctttag gtcatccatt aggtgctaca ggcgcaatgt taaccgcgcg
    44341 tttacttaat gaaatgggta gacgtcccga tagccgttac ggcatggtta cgatgtgtat
    44401 tggtgtcggc atgggtgcag ctgctatatt tgaatatgtg cgttagaatg gttgattttg
    44461 gatgaagcgg attcgttttg ttattgaatg aagtaggctg aagttgaagc cagttgaagt
    44521 tgaagcgggt tgaagcaatt tcgttttatt aatgaagctg tgtgaaatat agtgattgaa
    44581 caaaaaagtg gtttaatggg atggtggtta tttccgtttt agaatttaac atttacacgt
    44641 ctaattttaa tcattgtttt aaattttatg aatcgaagcc ctttgattta ataatatttg
    44701 ctaatgctag taacttatct gattgttcat gtttaaaata aagaaaacca ctcacatcag
    44761 tgtgtgttcg aactagactt gtaagttcca gttcggcacg actttctaaa gcaattatta
    44821 ttgctgtgat tgtcgtatat cacttagatg tgcgtggttt attttaata
//
LOCUS       DANRDI010000004        43920 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.004, whole genome shotgun sequence.
ACCESSION   DANRDI010000004 DANRDI010000000
VERSION     DANRDI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 43920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.004"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..349)
                     /locus_tag="PZ377_000092"
     CDS             complement(<1..349)
                     /locus_tag="PZ377_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDM6356985.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKK"
     gene            complement(366..755)
                     /gene="nusB"
                     /locus_tag="PZ377_000093"
     CDS             complement(366..755)
                     /gene="nusB"
                     /locus_tag="PZ377_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDM6356986.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            complement(815..1177)
                     /locus_tag="PZ377_000094"
     CDS             complement(815..1177)
                     /locus_tag="PZ377_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDM6356987.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            complement(1192..2547)
                     /gene="accC"
                     /locus_tag="PZ377_000095"
     CDS             complement(1192..2547)
                     /gene="accC"
                     /locus_tag="PZ377_000095"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDM6356988.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            complement(2547..3011)
                     /gene="accB"
                     /locus_tag="PZ377_000096"
     CDS             complement(2547..3011)
                     /gene="accB"
                     /locus_tag="PZ377_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDM6356989.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            complement(3485..4042)
                     /gene="efp"
                     /locus_tag="PZ377_000097"
     CDS             complement(3485..4042)
                     /gene="efp"
                     /locus_tag="PZ377_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDM6356990.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(4068..5129)
                     /locus_tag="PZ377_000098"
     CDS             complement(4068..5129)
                     /locus_tag="PZ377_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDM6356991.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            5234..5815
                     /locus_tag="PZ377_000099"
     CDS             5234..5815
                     /locus_tag="PZ377_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6356992.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            5829..6047
                     /locus_tag="PZ377_000100"
     CDS             5829..6047
                     /locus_tag="PZ377_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6356993.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6111..6941)
                     /locus_tag="PZ377_000101"
     CDS             complement(6111..6941)
                     /locus_tag="PZ377_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDM6356994.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            7099..7485
                     /locus_tag="PZ377_000102"
     CDS             7099..7485
                     /locus_tag="PZ377_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDM6356995.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            complement(7850..9322)
                     /gene="gcvPB"
                     /locus_tag="PZ377_000103"
     CDS             complement(7850..9322)
                     /gene="gcvPB"
                     /locus_tag="PZ377_000103"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDM6356996.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(9315..10661)
                     /gene="gcvPA"
                     /locus_tag="PZ377_000104"
     CDS             complement(9315..10661)
                     /gene="gcvPA"
                     /locus_tag="PZ377_000104"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDM6356997.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            complement(10681..11772)
                     /gene="gcvT"
                     /locus_tag="PZ377_000105"
     CDS             complement(10681..11772)
                     /gene="gcvT"
                     /locus_tag="PZ377_000105"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDM6356998.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     regulatory      complement(11847..11944)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(11931..12455)
                     /locus_tag="PZ377_000106"
     CDS             complement(11931..12455)
                     /locus_tag="PZ377_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDM6356999.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFDFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     gene            complement(12445..12591)
                     /locus_tag="PZ377_000107"
     CDS             complement(12445..12591)
                     /locus_tag="PZ377_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357000.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            complement(12688..13185)
                     /locus_tag="PZ377_000108"
     CDS             complement(12688..13185)
                     /locus_tag="PZ377_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDM6357001.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
                     KTIYV"
     gene            complement(13103..13402)
                     /locus_tag="PZ377_000109"
     CDS             complement(13103..13402)
                     /locus_tag="PZ377_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDM6357002.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            complement(13389..13835)
                     /locus_tag="PZ377_000110"
     CDS             complement(13389..13835)
                     /locus_tag="PZ377_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDM6357003.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            complement(13813..14124)
                     /locus_tag="PZ377_000111"
     CDS             complement(13813..14124)
                     /locus_tag="PZ377_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDM6357004.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(14138..15208)
                     /locus_tag="PZ377_000112"
     CDS             complement(14138..15208)
                     /locus_tag="PZ377_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDM6357005.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(15180..16154)
                     /locus_tag="PZ377_000113"
     CDS             complement(15180..16154)
                     /locus_tag="PZ377_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDM6357006.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKRTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            complement(16206..16829)
                     /locus_tag="PZ377_000114"
     CDS             complement(16206..16829)
                     /locus_tag="PZ377_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDM6357007.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(16826..17155)
                     /locus_tag="PZ377_000115"
     CDS             complement(16826..17155)
                     /locus_tag="PZ377_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDM6357008.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(17155..18141)
                     /locus_tag="PZ377_000116"
     CDS             complement(17155..18141)
                     /locus_tag="PZ377_000116"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDM6357009.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(18138..18341)
                     /locus_tag="PZ377_000117"
     CDS             complement(18138..18341)
                     /locus_tag="PZ377_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDM6357010.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(18322..19785)
                     /locus_tag="PZ377_000118"
     CDS             complement(18322..19785)
                     /locus_tag="PZ377_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDM6357011.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            complement(19797..20336)
                     /locus_tag="PZ377_000119"
     CDS             complement(19797..20336)
                     /locus_tag="PZ377_000119"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDM6357012.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            complement(20545..20694)
                     /gene="rpmG"
                     /locus_tag="PZ377_000120"
     CDS             complement(20545..20694)
                     /gene="rpmG"
                     /locus_tag="PZ377_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDM6357013.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            complement(20807..22882)
                     /locus_tag="PZ377_000121"
     CDS             complement(20807..22882)
                     /locus_tag="PZ377_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDM6357014.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            complement(23003..23602)
                     /locus_tag="PZ377_000122"
     CDS             complement(23003..23602)
                     /locus_tag="PZ377_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDM6357015.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(23879..24289)
                     /locus_tag="PZ377_000123"
     CDS             complement(23879..24289)
                     /locus_tag="PZ377_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDM6357016.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(24276..25139)
                     /locus_tag="PZ377_000124"
     CDS             complement(24276..25139)
                     /locus_tag="PZ377_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDM6357017.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(25181..25966)
                     /locus_tag="PZ377_000125"
     CDS             complement(25181..25966)
                     /locus_tag="PZ377_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDM6357018.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(26092..26982)
                     /locus_tag="PZ377_000126"
     CDS             complement(26092..26982)
                     /locus_tag="PZ377_000126"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDM6357019.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(26992..28338)
                     /locus_tag="PZ377_000127"
     CDS             complement(26992..28338)
                     /locus_tag="PZ377_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDM6357020.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(28452..29552)
                     /locus_tag="PZ377_000128"
     CDS             complement(28452..29552)
                     /locus_tag="PZ377_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDM6357021.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            complement(29555..30232)
                     /locus_tag="PZ377_000129"
     CDS             complement(29555..30232)
                     /locus_tag="PZ377_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDM6357022.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(30363..31469)
                     /gene="rpoD"
                     /locus_tag="PZ377_000130"
     CDS             complement(30363..31469)
                     /gene="rpoD"
                     /locus_tag="PZ377_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDM6357023.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(31693..33492)
                     /locus_tag="PZ377_000131"
     CDS             complement(31693..33492)
                     /locus_tag="PZ377_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDM6357024.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(33553..34371)
                     /locus_tag="PZ377_000132"
     CDS             complement(33553..34371)
                     /locus_tag="PZ377_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDM6357025.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(34382..35005)
                     /locus_tag="PZ377_000133"
     CDS             complement(34382..35005)
                     /locus_tag="PZ377_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6357026.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            35340..36731
                     /locus_tag="PZ377_000134"
     CDS             35340..36731
                     /locus_tag="PZ377_000134"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDM6357027.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(36881..37627)
                     /gene="recO"
                     /locus_tag="PZ377_000135"
     CDS             complement(36881..37627)
                     /gene="recO"
                     /locus_tag="PZ377_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDM6357028.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIGEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(37655..38554)
                     /gene="era"
                     /locus_tag="PZ377_000136"
     CDS             complement(37655..38554)
                     /gene="era"
                     /locus_tag="PZ377_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDM6357029.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(38555..38959)
                     /gene="cdd"
                     /locus_tag="PZ377_000137"
     CDS             complement(38555..38959)
                     /gene="cdd"
                     /locus_tag="PZ377_000137"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDM6357030.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVPAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(38970..39314)
                     /locus_tag="PZ377_000138"
     CDS             complement(38970..39314)
                     /locus_tag="PZ377_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDM6357031.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(39317..39784)
                     /gene="ybeY"
                     /locus_tag="PZ377_000139"
     CDS             complement(39317..39784)
                     /gene="ybeY"
                     /locus_tag="PZ377_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDM6357032.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(39785..40732)
                     /locus_tag="PZ377_000140"
     CDS             complement(39785..40732)
                     /locus_tag="PZ377_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDM6357033.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(41036..41734)
                     /locus_tag="PZ377_000141"
     CDS             complement(41036..41734)
                     /locus_tag="PZ377_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDM6357034.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(41751..42740)
                     /gene="floA"
                     /locus_tag="PZ377_000142"
     CDS             complement(41751..42740)
                     /gene="floA"
                     /locus_tag="PZ377_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDM6357035.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(42758..43441)
                     /locus_tag="PZ377_000143"
     CDS             complement(42758..43441)
                     /locus_tag="PZ377_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDM6357036.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(43685..43861)
                     /locus_tag="PZ377_000144"
     CDS             complement(43685..43861)
                     /locus_tag="PZ377_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDM6357037.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT        13861 a         8040 c         5887 g        16132 t
ORIGIN      
        1 ttttctttaa tgcttctaat ttttgatata agttccctat accatctaat tgcattttat
       61 ttacataaat ttgatagttt ccacgacgtt caaaaacaga aacacgtgct tctaataaga
      121 cttcatctcc ttctttaggt tcgaagttta atttagaagc actacctttg aacatcatgg
      181 cacttataac gctttcttta tctttcacat taaagtataa atgaccactt gaatgctttt
      241 tgaaatttga aagctcacct ttaatcaata cagattggag atgtggatct tgatcaaatt
      301 tatatttaat atatttcgtt aaagctgaaa cacttaaata atctgacata taacatcact
      361 caattttatt tttttatatt actcaataca ccatttataa atttataatg atcatcatca
      421 ctgaattgtt ttgttaattc aactgcttca ttcattacga ctttagcagg tgtatcactg
      481 tgtaatattt catatgttgc cattcttaaa ataatacgat ccgtttttaa taaacgtgca
      541 atagtccaat cttttaaata aggactaatt gtctcgtcta atacaggttc gtgaccttta
      601 acgccagaaa ctagccaatg aataaattcg aagtctaaat ctggattatc gtctttaata
      661 aagcttatcg cttcatttat cgttaaatca ctgtccttca tttctaattg aaataaagtt
      721 tgaaaagctt gcactcggga ttctttacga ctcattttta actccttcaa acgtttgtat
      781 ttttctttat ttaattactg aattaggtat gacattactt ttcaataacg atttgtgtaa
      841 tgtgaatatt aatttgctta ggttctatcg ctgtcatatt agaaattgaa ttaaaaattg
      901 acgtttgaat tttgtttgca gtttttgaaa tattaacacc atgttttaat gcacaatata
      961 catctatata tatgccatct tctttactct cgatttttaa atcacggctt aaatttttac
     1021 gactaacttt ttctaaattt gtttctttta attcagcaaa atggccagtg atgccttcga
     1081 cttccgaagt agctatactt gcaataacag atagcacttc tggcgctatt tctactttac
     1141 ctaattttga atttgaataa tcagttactt tgaccatgga ttgacctcct attaaccttc
     1201 atcattcata atgctatttt gctctaaaaa gtttgtatta aatttaccgc ttctaaatat
     1261 atcgttattc aataatttaa tatggaatgg aatagttgta tcaataccaa gaaccacaaa
     1321 ttcacttagt gcacgaatgc cagccataat cgcttcatct cgtgtcggtt catgtatgat
     1381 taatttcgct accatcgaat cataatatgg cggtatcgta taattagtat aacatgctga
     1441 ctctattcga acaccatatc cacctggtgc aagatattgc tcaattttac ctggtgatgg
     1501 cataaagttc ttgtaaggat tttcagcatt aattctaaat tcaattgcgt gtcctgttaa
     1561 tttaatatct tcttgtttat acggtaacac gtcacccata gcaacttgta attgtaattt
     1621 aactaaatca attcctgtta ccatttcagt tacaggatgt tctacttgaa tacgtgtatt
     1681 catttccata aaataaaatt tattatcatt taaatcatat ataaactcaa ttgttcccgc
     1741 attttcataa tttacagctt tcgctgcacg aactgcggca tttcccattt cacgacgtgt
     1801 ttcatcatct aaaattgggg aaggtgcttc ttccactaat ttctgcatac gtctttgaat
     1861 tgtacaatca cgttctccta aatgaattac attaccatag ctgtccccaa caatttggat
     1921 ttcaatatgg cggaagtttt cgatgaattt ctccatataa agtccaccat taccaaatgc
     1981 agtttgagct tcttgttctg tcattcggaa gccagtttca agttcttttt catcacgagc
     2041 aacacggata ccttttccgc caccgccagc agtagcttta atgatgaccg gatagccaat
     2101 ttttttggcg attttcttag cttctgagac gtctttcatt aaaccgtcac taccaggaac
     2161 aactggaaca ttggctttga tcatttctgc cttagcaaca tctttgatac ccattttttg
     2221 gatagattga taacttggtc caatgaactt caattggcat gcttcgcata attctgcaaa
     2281 atcagcattt tcagctaaaa agccataacc cggatgaacg ccatcacaac ctgtagaagt
     2341 tgcaatagat aagatgttcg gaatatttaa atatgaatct ttagacaaag tgggacctac
     2401 gcaatatgct tcatcagcaa tttgagtatg tagcgcatct ttatcccctt cagaatagat
     2461 tgcaacagtt tggatgccta aatcacgaca agcgcgaata atcctaactg cgatttcacc
     2521 gcggtttgca attaaaacct ttttcattat ttcaccttaa ataacggttg gccatactct
     2581 accatttgtc cgtcttctac taagatttca acaatttcac ctgaaatttc tgcttgaatt
     2641 tcattaaata gtttcattgc ctctaaaata cacactgttg tttcatttga aacagtgtcc
     2701 ccaacttgca catatgcttc ttcgtctgga gatggcgatt tgtaaaatgt acctaccata
     2761 ggtgcattaa ttgttttgtg attatctgaa gttggctttg gagcttcagt tttattgcta
     2821 tcagttgatt gtgcttgagg cataggcatt gccgcagctt caactggcat ttgtgagatt
     2881 tgtggcgtga taatctcagt ttctttttct ttcttaagcg tcactttgcc tttagtatct
     2941 tcaatattga tttccgttaa agttgattta tccagaattt caattaattc tttgatttct
     3001 ttaaagttca ttattactga ctccttcagt ttgttttcat ctacccgtct attttacttg
     3061 agacaactct tcaattcaag catgttcata ttgctggcga cattataagt ctatcccaaa
     3121 gttataataa aaccacattt taaattaaaa acacttgtgt atttattact taacattgac
     3181 tcatcttaac tcttgattaa aaattttatg cattcagtta aacatatcaa tgttattttt
     3241 attagctatt atgttttatc ctcaacaata tgatatgtag atatatacct ctactcatgt
     3301 ctatgttttt tcactaccaa ttagacgatt cgttttatat tttaaaaatt attttaaaaa
     3361 taaaaagcct catatcagca tataacctat aatcatttta ggcaatagct ttatgagact
     3421 tatttagaat atcttacgtt aaatcagtat agtgaataca agctatttgt taacaaatta
     3481 gagattatcc tcttgaaatg tagcttccat caccagtgtt gataattaaa acgtcacctt
     3541 cgtttacaaa taaaggtaca tttaatgtat aaccagtttc aacagttgcc gatttagtgg
     3601 caccagttgc agtatcacct ttaataccag gttctgtttc agttactgtt aattcaacag
     3661 ttttaggtaa ttcaacaccg atagtttcac cttcgtatgt ttgaatttgt acttccatac
     3721 cttcttttaa gtaattcaat tcttctttta agtaatcact tgaaagttct gtttgttcaa
     3781 agctttcatt atccataaat acatgattat ctccgtcagc atataaatat tgcatgcgac
     3841 gattttcaat cattgctggt tcaacttttt caccagctct aaacgttttc tcttgaattg
     3901 caccagttct taaattacgt aattttgaac gaacgaatgc tgaaccttta ccaggcttta
     3961 catgttggaa gtctataact ttccaaatag cgttatcaac agaaattgtt aaacctgttt
     4021 taaaatcatt aaccgaaatc attcagtttc ctcctcattt tacacgctta tgttaaaact
     4081 ataaggtctt ttgtgcattt agtaaagact tgacaaccat tttctgtaat taaaatatca
     4141 tcttctattc ttataccgcc caaaccttct atataaacac caggttctac tgtaacacag
     4201 ttgttaactt gaagtttatc ttgtatcgta cgagccagca ttggcccttc atggatttct
     4261 aaaccaatac catgtcctag tgaatgtcca aattcttttc catacccttt tgactctaaa
     4321 tagtttcttg aaatggcatc agcttctgca ccagtcatgc caggtctaat ctcattaatt
     4381 gctttcattt gagattcaag tactatttga tatatttctt tcagtttagg atctggttct
     4441 ccaatagcaa atgttctagt aatatctgaa caatagccgt tataatacgc gccaaaatct
     4501 aatgtaatca tgtcgccttt ttcaataatt ttatcacttg caacaccatg tggtaatgca
     4561 cctctatgac cagatgctac aatcgtatcg aatgatggtc catctgctcc taattctagc
     4621 attttgcttt ctaatattgc ctttaattct ttttcagtca tgcctgcttt tacaacagtt
     4681 aaaatatatt catatgtttc atcaacaata ttagctgctt tttgaattaa agcaatttcg
     4741 tcagcatctt tgacgtctct aattttatct acagtattag aaatgcttat taatgatata
     4801 cggcttttat ttaattcaag gtatgtatca taacttacat gatgcccctc aaaacctaca
     4861 ttttcaaaat tttcttggtg tagcaattct ttaatctcac caataatagt agatttacga
     4921 ttaataattt cataatttgg cgcctgctta gttgcttgat caatatatct aaagtctgtt
     4981 atcaaatatt gtttatcttt agatatgata agtgctccac tggtaccagt aaaacctgat
     5041 aaatatcttc tattgtaatc cgaaagaatg ataatcgcat ctaaatgttt ttgttctaaa
     5101 atacgatgca cttgtgttat tctgctcatc ttattacctc ctagtgaaac gtttgtgcat
     5161 ttcaacttta tacattaaaa taatatcata ataaggataa aaaataatag atattgattt
     5221 tagggagata gtaatgaaaa aattggtttc aattgttggc gcaacattat tgttagctgg
     5281 atgtggatca caaaatttag caccattaga agaaaaaaca acagatttaa gagaagataa
     5341 tcatcaactc aaactagata ttcaagaact taatcaacaa attagtgatt ctaaatctaa
     5401 aattaaaggg cttgaaaagg ataaagaaaa cagtaaaaaa actgcatcta ataatacgaa
     5461 aattaaattg atgaatgtta catcaacata ctacgacaaa gttgctaaag ctttgaaatc
     5521 ctataacgat attgagaaag atgtaagtaa aaacaaaggc gataagaatg ttcaatcgaa
     5581 attaaatcaa atttctaatg atattcaaag tgctcacact tcatacaaag atgctatcga
     5641 tggtttatca cttagtgatg atgataaaaa aacgtctaaa aatatcgata aattaaactc
     5701 tgatttgaat catgcatttg atgatattaa aaatggctat caaaataaag ataaaaaaca
     5761 acttacaaaa ggacaacaag cgttgtcaaa attaaactta aatgcaaaat catgatagga
     5821 gtcttttaat gcgtaatata atattttatc ttgtacttat tattgctgcg attggattag
     5881 taatgaatct agatgccttt attttttcaa tcgtcagaat gttaatcagc tttgctgtaa
     5941 tagctggtat tatttatctg atttattatt tcttcatctt aactgaagac caacgcaaat
     6001 atcgcaaagc aatgcgtaag tataaaagaa atcaaagaag aaaatagata aaaaaacgga
     6061 agcacttgta ggtaaaatag tctacgtgct tccatttttt attctaaaaa ctactttcta
     6121 aacatccatt catctgaacg atatttttca gttaattctt ccacttctgc caattgagct
     6181 tctgttaatt caagtggctt aaattctata tttaaacctt tcttaaaacc tttctcgaaa
     6241 gcttcttcca tttgactaat agtaatgtgt tcatctgaaa tatcattgat ggcaactgct
     6301 ttttcaacga atgcctcttt catttttaat tttaatcttt catttttata aataaacata
     6361 tcaaacagtt catcaatatc aatatcttgt aaaatcgaac cgtgttggag gattacgccc
     6421 ttttgtctcg tttgagcact cccagcaatc ttacggcctt caacaactag ctcataccaa
     6481 cttggtgcat caaaacacac tgaacttcga ggttgtttta atttttgacg ctcttcaggc
     6541 gttttaggta ccgcaaaata agtatcaaat cctaagtttt taaatccttc taataatcct
     6601 tgtgaaatca ctctgtacgc ttctgtaact gtagaaggca tattcggatg cgattcaggc
     6661 acaatcacac tgtaagttaa ctctttatca tgtagcaccc cacggccacc agtttgacgc
     6721 cttacgagac caaaaccttt ctctttaacc ttatcaatat caatttcttt ttgtagcctt
     6781 tggaaatacc ctattgataa tgttgcagga ttccatgtgt aaaaacgtat aactggatca
     6841 atttcacctc tagagacaaa atttaataac gcttcatcca ttgccatatt ataatatggg
     6901 tctttacttc ctgtattaat aaaattccaa gtttcagtca tattcaagaa ctcctatcag
     6961 caaaatgtat aatatttgat tcgctaatta atcaatttaa ctaaatgaat aataattgca
     7021 attctttagt gaaatatttt gataatttga cctaacagtc ttataattat attatcgttt
     7081 aattagggag gatgcaagat gagtgctagt ttgtacatcg caataatttt agttatagca
     7141 attattgctt atatgattgt tcaacaaatt cttaacaagc gagctgttaa agaattagat
     7201 caaaatgaat tccataatgg gattagaaaa gctcaagtca tcgatgttag agagaaagtt
     7261 gactatgact acggtcacat taatgggtct cgcaatattc ctatgacaat gttcaggcaa
     7321 cgattccaag gattaagaaa agatcaaccg gtatacttat gtgatgccaa tgggattgct
     7381 agctatagag ccgctcgtat tttgaaaaag aatggatata cagatatcta tatgttaaaa
     7441 ggcggctata aaaaatggac tggaaaaata aagtctaaaa aatagttttt gtaaatttaa
     7501 tatacgattt aataaaatct gagtgttaat tgatcatcaa taacaatact cagattttaa
     7561 ttttttaaca aagtctgtta ctatatttct ctagcttcac tgatcattaa acttagtttc
     7621 agcataataa agaaagttca gctcattttc aatacgattc aattaccgca atctaaaaaa
     7681 tgaaaagaca atttctatga aagaataata ccaaacccta agagttatta cttcggttta
     7741 gttttcttgt ttaaatagaa attgtctttt tcaattgatt ttgaaaccat tatccttaaa
     7801 tcttcataca aagttagaat aataattctc ggaatatgtg tttaatactt tatttttcct
     7861 gtttaagatt ttcaaacttt aatattggtt tacgagcagc tgtagcttcg tctaatcgat
     7921 caatcacagt tgtatgtggt gcttctagca ctttatcagg atcattttta gcttcttcag
     7981 caatactaat taatgtatcg ataaaataat caagtgtttc tttagactct gtctcagtcg
     8041 gttcaatcat cataccttct tcaacattta atgggaagta tattgttggt ggatgtacac
     8101 cgaaatctaa taatcgctta gccatgtcta aagtacgtac accaaattct ttttgacgca
     8161 caccacttaa cacaaactcg tgtttacaat attgtttata aggtatttca aagtgtttag
     8221 ataaacgtgc tttaatataa ttcgcattaa gaaccgctgc ttcagaaacc tctttaagtc
     8281 cagttgctcc catagttcga atatacgtat aagctcttaa gtaaatacca aagttaccat
     8341 aaaatggttt tacacgtccg atagaatttt taatgtcatt atcatattta aatttgtcgc
     8401 catctttaat aaccattggc tttggtaagt aacttgctag ttcttttact acaccgactg
     8461 gacctgaacc aggaccgcca ccaccatgtg gaccagtaaa tgttttatgc aagtttaaat
     8521 gaacagcatc aaatcccata tctcctgggc gaactttgtc cataatagcg tttaaattcg
     8581 caccatcata atataataga ccaccagcat tatggacgat ttcacggatt tccataatat
     8641 ttttttcgaa aatacctaaa gtgtttggat tagttaacat aatagctgct gtattttcat
     8701 ttacaacacg tttcaagtca tcaatatcaa cttcgccacg ttcgtttgat tttacagtaa
     8761 ctgatttaaa tcctgcaaat gaagctgagg ctggattcgt accatgcgca gaatctggca
     8821 caatgacttc atcacgatga ccttcaccat tattctcatg gtaagcttta aatatcatca
     8881 atgcagtcca ttcaccatgt gcgccagcag ctggttgtaa tgtcacctca tccataccag
     8941 taatttcttt taattcttct tgcaaactat aaataatttc taatgaacct tgaacttgat
     9001 cttcatcttg taatggatgt gattcactaa atcctggtat tctagcaacc ttttcattaa
     9061 ttttagggtt atacttcatc gtacatgaac ccaatggata aaatccgttg tctacaccga
     9121 aatttttatt tgaaagttca gtataatgac gtactaagtc tagttcagca acttcaggaa
     9181 actccgcttt gtttttacga ataaatttat catctaacaa tgactcaaca gaatttgttt
     9241 taatatcact ttttggtaat gaatatgcat atctgccttc acgagatctt tcaaaaatta
     9301 atggacttga tttactagtc atttaactca ccagcctttt ctacaaatgt atcgatttca
     9361 tcttttgttc ttaattcagt tacagctatt aacatgtgat ttttaaagtc gtctgaaaca
     9421 acacctaaat caaaaccacc gataatattg tacttcacta attcctcgtt aacttgttga
     9481 attggtttgt caaatttgac tacaaactca ttgaaagatg taccatctaa tacttcaaaa
     9541 ccttttttaa taaattgttg tttagcatag ttagcatgtt ctatattttg aactgcaata
     9601 tcatagatac cttgtttacc aagtgctgac attgcaattg atgacgctaa agcatttaat
     9661 gcttggttag aacaaatatt agatgtcgct ttatcgcgtc gaatatgttg ttcacgtgct
     9721 tgtaatgtta atacaaagcc acgattacct tcatcatctt gtgtttgacc gactaatcta
     9781 cctggcactt tacgcattaa ctttttcgtc gttgcaaaat atccacaatg tggcccaccg
     9841 aattgagcag gaattccgaa tggctgagta tcacctacaa caatatctgc accaaatgaa
     9901 cctggaggtg taagtaatcc caatgctaat ggatttgcat atacgataaa taatgctttt
     9961 ttatcttcaa taaagctatg aatcttttca agatcttcaa ttgaaccgta aaagtttgga
    10021 tattgtactg caacagctgc tgtttcatca tccactgctg cttctaattt tttcaaatct
    10081 gtaacagtgc catctaaatc gatttccact acttcgaatt ccttacgcgt cttagcataa
    10141 gtatgaagta cttgtaatgc ttgataatgt aaaccttttg agactacaat tttatttttc
    10201 tttgtttgac taaatgctaa gatacatgct tcagcaaagc tagtcatccc atcatacata
    10261 gaagaatttg ctacatccat atctgttaat tcacaaatta aagtttggaa ctcaaaaatg
    10321 gcttgtaatt caccttgaga aatttccggt tgatatggcg tatatgctgt gtaaaattct
    10381 gatcttgaaa tcatagcatc cacaactgat ggcgcgtaat gatcataaac accagcaccc
    10441 aaaaatgatg tatgcgtttc tttagtgata ttcttgcttg caatgcgatt taatcttcta
    10501 agtaacgttg tttccgcttc gccttcagca atatttaaat ctctatttaa taaaatgtca
    10561 cttggtacat caccgaataa ttctcctata gattttgcac caattgtttg taacatttct
    10621 tgcttgtctt tttcagttaa aggtatataa cgatgactca caatgcacac ccctttttaa
    10681 ttatttatca atttgatttt tcttaacaat tttcgctttt aattgacgct tacgaacttg
    10741 aacaagcaac tctctaccca tttcaaactc atctcttttt atcattgcaa gtgcaattga
    10801 ttttcctgat gatggagact gtgttcctga agttacttct ccaataatat ttccatctaa
    10861 atccataact tcataaccag ttcttgcaat tcctttttca agtaattcta atcccactgt
    10921 tcttcttggt gcaccatttt ctttttgatc ttttaataca gatttaccaa taaaatcagc
    10981 atcaattaat ggtttacttg caaaagcgat accaccttca tatggtgtaa ttgattcagt
    11041 taaatcttgt ccatgtaatg gcaatccagc ctctaatctt aatgtatcac gagcgcctaa
    11101 cccacatggc ataacattat actctaataa accatcccaa attttttcag tatcatcgat
    11161 attacaataa atttcaaacc catcttcacc tgtgtaacct gactgagata aaatgacgtt
    11221 tgctccaaat aatttgacac cctgtttaaa ttcaaacatt ttcatttcag ttacatcttc
    11281 atcaactaat tgattaatta aatctctagc ttttggtcct tgtattgcta attgaccata
    11341 ttggtttgat acattttgta cttcaacatc aaatttctct ttgtgtttta aaatccaatt
    11401 aaaatctttt tcagtattag cagcattaac aactaataaa taattgtcgt cagctaattt
    11461 atatattact aaatcatcaa taataccgcc ttcttcatta cataaagcag tatataatgc
    11521 ttttgaagta gttaaattat cagtatcatt tgataataaa tattgcacaa actgactagc
    11581 atctttacct gttacttcaa tttcacccat atgactaaca tcaaacaggc caatttcgta
    11641 tcgaacagca ttatgctcct ctttaatact tgaaaattga acaggcatcg cccatcctcc
    11701 gaattccaca atttttgcac ctctatcaac ataattttga tataaaggtg tttgttttaa
    11761 atcacttgac attgaaatac ccccttaaaa ttataaaaac aggatagcaa tcgctaccct
    11821 gtttataaat caccaattca ggaacgcgtt acagtattat ccttttgcct gagagtttcg
    11881 taataaatta cttgcacctt cggcgataga gtcttaacgc tctattctct ctaatactga
    11941 tcactcgctt ttattaaatt tagcaattca taatatattt ctgaaattga tagcaaatga
    12001 ctatcaaatt tcttgaatgc gatttcatta tatcttaaat tccgcgagca atacaagtca
    12061 tttaactgct tgattgtctt attatttgca ttaggtcgat gtgggtcatc attgattcga
    12121 ctatatataa tatcaatatt acaatctaac caaataatgt ttttttgatt tttcaaaaaa
    12181 tcaaatgcct cttcactctc aataatacca ccaccagtag caattatatc tgcagtgtta
    12241 atacattctt gcaaacatgt gaactctaaa ttcctgaaat attgttcacc atgtttacaa
    12301 aatatttctg gtattgttaa cttatacttc tcttcgatat atgaatctat atcaataaat
    12361 gataaatttt gctcatctgc aacgtattta ccaatcgtag atttaccggt acccatgaaa
    12421 ccaattaaaa ttattggtga tttatcatga ttcatgcgtt ttcccttctt tcaataacaa
    12481 aacaatctta tagttatagt cagtttcaat attatataat gtctttaatt ttaaactata
    12541 ttgactgata taaaaagtga aaatggaaaa ataaattgct gttagcatca ataagaacgg
    12601 cgtaacatat cctttttctt tatataaatc aaaattaata aattatttca catccttttt
    12661 aatagtactc atttaaatac tagtaagtta tacataaata gttttagtct gcacatttgt
    12721 accgacttta actgttatcg ttattttaat aatggattta tagtagatat ttgcagtaaa
    12781 tgctgtaaca ttattaatca ttgttatatt tcctctgtca tttacaactt taataatttt
    12841 attattaatt aatttgtatt cgatcatttc ttcaccttta tgtaaaatga tacggtgttg
    12901 cctaatttca atatcgtttc tatctactcc tttaaaatca tctagaatat ctcttgagaa
    12961 aaattcgaaa tctacagttg ttaaatccct actttcaatt agaaaagttt tactaagtct
    13021 aattaagtct ggaacaatta gtaaagttat acttataacc atcatcgcta ctaacatttc
    13081 aatgagcgaa aaagctttga cattaatact gtatacatgt cttttgataa gaagtggtat
    13141 tttttgaaat agcacaaatt tgttggtcac tttgcttaat atcatacttc cctatagtta
    13201 cccccttctt aagttcttct ttattaattt tagatacagt cgtcaaaatt actttagaag
    13261 catcaattgt ttgtagttta tggtttatac tcgcttgcat ttgattcatc attggtatca
    13321 atagtaatgt aatcaatcca attagcaaaa atccagccat actatctatt aagaatgaac
    13381 ctttacactt atagcttttc ataacgaatt cttccttttt caatatggaa tattattcta
    13441 taaattgaat tgttattgta aattgttatg ctaccaaatt tattgatatt ccctttttta
    13501 tcaaaggcaa taatatcaac ttttgcaaca ttaattattt tgcctacttt taattttaga
    13561 aatcgtatat tattattctc tattacttta atagtgtcac tgttttcata aaatctaaca
    13621 ttgatatatc cttgatttgc tatagcttgc gacttaatat aattcaattc agtaataaaa
    13681 gaaatgatat ttgcctcatc atctattact ctaagattgc ttaatccttt agatgtcatt
    13741 gtcaaaagta gaaatatact gattaacatc attaccacaa gcatctcaat catagtaaat
    13801 gctgactgct ttctaatttg caactgcttc tccattacta attgtgattg tctctcctga
    13861 tttacatgtc ttttgtgctt cttttataaa accatctgca attaagtctt caatagacga
    13921 tggatttcta ttatgtttca atgcatacgc ttcaatttga ctattaacca tttttacctg
    13981 tgcattacaa cctgttgatt gtatgtgagc agtttgttta gcaatatttg gaatgattaa
    14041 aattaataat aaactgatga ttaataacac taatagcatc tctatcaatg taaacgcttg
    14101 agttttctta agaaatttaa acatatatta acctccatta ttttatactt tgcatcattt
    14161 gaaacattgg taacataatt actaaataaa ttgcgacaat aaataaacct aaaatcaaaa
    14221 ataaaatagg ctgtaaaaac tgagtctgtt ttatcgcttt atcttctatt tgttttacta
    14281 atatttgcga atataacttt agttctactt ctagcttccc tctcttttca ccttgtagca
    14341 caaacttaat taattgaggc ttaaagcatt ttagtttttc taaaatttga ggcaaaccat
    14401 atcccatttc tgaataagtt aataagtatt tacctagaaa ctgtctaaat ggatcactac
    14461 tatggttaat ataaacgtct actattgatt gaagtgtaat accattttta taaaacaaca
    14521 ctaattcatt agttacaaaa taagttttaa ataattggaa atagcctgat attagcggta
    14581 gtttcatcac aaagtttatc ttattgagca tatttaaatt gttataaatt aatttcataa
    14641 taatagccaa catagatact attatgagca ttactacaat tatagtaggt aagctggtaa
    14701 tgaaaaaaga caatgttttt tgaaaagaag atagttgaat attcatagaa gtatataatt
    14761 gttgaaactg tggaattact gtgaggttta atataataat catagcaata aagatagaaa
    14821 ctagtattaa ggggtattgc agtgtcttta acaatcgttg ttctgacttt ctattcactt
    14881 tcatataatt tacggtttct tctagaacgt ctataatatt gccaaatctt tctgccaaat
    14941 atacttgcat gacgatagta tcgctataac ctatcagtga taatatttga ttgcatggtg
    15001 caccatttga aatttcactt agaatggtgg tacctaattg cttattttta tatgtcattt
    15061 gaagatttaa aaattgaaaa ctttgataca gagtgaaacc atatttcaac aaattacata
    15121 aatttgaaag taagtcgatt tgttgggcct tacttaattg tcgcttctta gaatgtagtt
    15181 taaatgtatt tatgcattgt agtttcacaa atgacacctg cttttgtcat atcatcaagt
    15241 ttatcttcta agttcttaaa tgatgatggt aatgaatgat tatgggaaaa gaaatatcgg
    15301 agttgttgct gagatagaat ttcacatact aattgtcgct gttgcttaat agtagttaca
    15361 agtcgttggt ttataattaa gttagttgcc tgtatcaatt cttgtacaga aatgcccatt
    15421 tctaatagcc ttaaaatagc acctttacaa tcagttgcat gcaatgtagt cagaacaagg
    15481 tgaccactta aactagcctg tataacacac ttggcaacat ctttatctct gatttcacct
    15541 attaaaataa catcaggatc acatcttaaa atagctttaa acgaatttac atagtttatg
    15601 ccagctttat cattcacatt aatttggacg ataccaggaa tttgcatctc tacaggatcc
    15661 tctatagaaa ttacatttaa attcaaggct ttattcgcgt atgagaccat ttgatacatt
    15721 aatgtcctct ttcctgaacc agttggtcca ctaaacaata gtaatccttg tttcttattc
    15781 atgaggtgtt taaaatcatt gaatttataa gttgatttct gtggttgaaa aaattgaggt
    15841 acaattctga taacacaact ttcttggcca agtgacaatg gtaaagttga tattctcaaa
    15901 aaatatattt tattgaaaag gtaactatat cgaccgctct gtgcgacttg ctgtgtagaa
    15961 acatcaagcc cagcttgaaa cttcatataa actaataact tttgataaat gctattccca
    16021 atttgttcat actgctccaa gttatcatta attctaaatt taatacttac ttcattttta
    16081 actggaataa aatgtacatc actcgctttc atttctatcg ctttattaat tatttcttga
    16141 aatagaatct tcaaaaaaac acctcctaca tataatcacg taggaggtgt ttttattact
    16201 tcaatttaac cgtgtaaaaa tggatttaat tgttcatcat caaccgtcgt atatggacca
    16261 tgtccaggga ataaaggtaa atcgccttct aattcaaata ttttatcttg aatagaatca
    16321 actagcgttt cataatcacc tttatataaa tctgtacgtc cgattccatt attaaataat
    16381 gtatctccaa caactgcgaa ttcatcgaac acatatgtta aacttcctgg tgaatgtcca
    16441 ggtgtgtgta acacattaaa cttaaatcct tctatttctg tgctaccttc gtttaacttt
    16501 tcaggagtta ccttacttgt aataattggt aatccatatt gcttaaattt atctgcccca
    16561 tttttaacgg gatcttttag aaaatcaaac tctgcttcat gcatataaac cgggacatcg
    16621 aatcgatcaa ctatatcatc gactgctccg atatgatcaa agtgtgcatg tgttaataaa
    16681 atagctttta acggtttatt tatttggttt aattttttaa taattttttc actttcacct
    16741 gaagggtcaa tcagaataac agctttgtca ttttcgatga aatacgtatt agtatcaact
    16801 aagcctaaag ttaagcttga aatcctcata ggttttcacc actattttct aaatgttttt
    16861 gtactgatgt tagtttatca ttcatttttc tagatttgtc tcgtcggtca tcaatacgga
    16921 tatttgtaca aactctactt aaacctttat caaaaggtaa ttcatgtatc acttgcacaa
    16981 cttctaatac atcgcttaat tcaccttcaa ttagagtatt cattggtgtt aattgaaaat
    17041 caattttacc cattgcttta tattcttgaa gttttttctg aatatctgca atatatttac
    17101 taacactcgg accttccgtt ccaactggaa taacaaccac atcaacaata gccattattt
    17161 taccccctct ttatctaata cataggtctt gattaatcct gctgctcctg taatacctgc
    17221 atcattacct aatttcgctt gtacaatttc agtttcaaat tgagcaggtg caaatgttaa
    17281 attatgatat tctgttttaa tattttcaat taaaataggt cctgcagtag acattcctcc
    17341 acctagaacg atatatttcg gattacttgt aacactaata atactacata aatatccaat
    17401 atagtttgca accttttcag taatgaaaat acagaattgg tcaccagctt ttgccgcatc
    17461 aaaaacagct tttgctgtaa ccttattttc tttaatcaat tctaatatag aagatctaaa
    17521 cgtcaacttc ggatagtaga agttaactaa gttaacaacg cctgtcgctg aagcaactgt
    17581 ttcaatacat ccagaacgac cacaattaca tttaaatcgt tgatcgaagt ctgctctaaa
    17641 atgacctatt tctgcgccag agccattatg accatgtacg atttcaccat ttgaaataat
    17701 tcctccacct agacctgtac caagtgtgat ggcaacaaca tcatcggcac cttcaccagc
    17761 acctttgtgt ttctccccta aagcagctat gttagcatca ttatctacat acactggaca
    17821 atcaacgaat tgttcaaaaa tctcacgtac attaactttt tctggccaat ataagtttac
    17881 tgctccattt actgtacctt tttcaaagtc aacaggacct ggtacaccaa tacctacgcc
    17941 aagtacattt gaaaaattat aattattttc atttactttt tcaacaaacg aatcataaat
    18001 tcctttcaaa agtgtatatc ctgtactatc agatgtatca gtgtgaatag accatttatg
    18061 taattgttct aattcaggtg tgaaaatacc taatttacaa gtcgtcccgc ctacatcagc
    18121 tgctaaaata atttttgtca tttctgttca ttccttctct gattaattat caacgtacat
    18181 ttcaaatatt cttccttaga taacagttca tattgataca atgatgaaat ctcctgttga
    18241 atcatttcgt acatatcttc tggattttta aaatatatta gaaatccgta acttttcaat
    18301 aactgttgta catcataaaa gttatttatt tttcgactca tttgatttag tcaactcttt
    18361 ttctaagtta atataatctg tatttttagg gtctgcattt aatgctttac gcacatattt
    18421 taatgctttt tcatcatcat ttaatgaacg atttgctatc gctaactcaa aatttaataa
    18481 acctgattta ggaaacattc taagtcctcg ctcccattct gtcatacctt cggacttaga
    18541 attgatagta gccataatca taccacttaa ataatatgtt tgatcatcgg cataattttt
    18601 atttattgtc tgcttcacaa tattttgagc attatcataa ttaccactag tcatatcatc
    18661 tttgatcaat ttattataaa tattatcttc tttaattgta aaaattctaa tttgaagtgc
    18721 aataaatata acaagcatac caattagtaa aatccaaaaa atattacgat tcactttata
    18781 gtaatagcca attaaagtta ttaataaacc accaatgaat cctccaatat gcgccacaat
    18841 atttatattt gacataaaca gagaaacacc aactaatatc actaatgcaa ttaataactg
    18901 tcctaacatt tttttgttaa atgtttttga aacatacatc atcgcaaaaa ttgatccaat
    18961 cagaccaaat atagcaccac tagccccaac tgaaattgta gtcgtattaa atgatagtga
    19021 tacaaagttt ccaaacaacc ctgcaataaa gtatacagtt aacatccgcc atgaaccaat
    19081 aattgcttcg actattttac caaaaataaa taatgaaagc atattcataa gtatatgttc
    19141 aaaactaaaa tgtaaaaaca tcgatgtaac aattcgatac cattcaccat gtacgacatt
    19201 aaaatgcact aacccgccaa catctaataa ttttacatcc gaaaaattat ttaaatataa
    19261 aatcatacat aaccatatta agacatttac aaatattatt gtgtatgttg ccggtgaaaa
    19321 tttctgcata tatttatcta aaaagttatc ggttaatact ttgcgtttat agaacatata
    19381 tgtcttttta tcattatctt ctgaaataag tctagccaaa aatatattcg gcatatgctt
    19441 tatcaaatct tttgtgtgtc ttataacatt gaatttaatt ctaattggcg aaacttcatt
    19501 taattgttct tctgaaaatt ccgattctgt aaaataataa aattcaaaac tttgtggttc
    19561 gaaagaaata aaatttgcta tttcatcttt gtgttcaagc actttcgctt tgtcaaaacg
    19621 tatttcttgg gtagacttga tatgttgttt aaaaatgact acttgttttt ttcttttatg
    19681 ggctaaccaa atttcttggt catctttttc cctactgaca atatcaaaat tcaagtacct
    19741 aatccaataa tatattgttt tccaaaattg tttgtctatg ttcatttagt cctccactat
    19801 gctgcttgat atataatcaa tttatcgact ggttgatcga atgattccgg ttcaaatgat
    19861 gttatttgaa aatcgtataa taagcttatt gtctttgtct gataattagc taaaaacctg
    19921 tcgtaatagc caccaccata cccaattcta tatccatcgt cttgaaatcc aacaccagga
    19981 acaacaatta aatctaggtt attcgttgtt tcaccttttg aagttggata gtaaatcccc
    20041 ttattatcga catcaatatc tttgagatta aatatctctt taaaagtcat ttgatgattt
    20101 aaataatcca ttttcggtac aaaaatacgt ttatgatcca ttaaggcttg ttcaataata
    20161 gaaaaagtat ctacttcatg attaaaagaa agaactagcg caattgcgtt tgcttctttg
    20221 tattcttcag ttgcaaaaaa ttgatttctt aaccatgtgt ctgcttttcg cttttcagct
    20281 ttattaaaat tcttcatttt atgtaaaatg tattttctaa tctcattttt agtcacatta
    20341 tcacccactt tattcaattc tattatatcg ttaataacaa tacatgtcat tatgttgtca
    20401 tgatgaatta tgcgacaatt ctaaaattta ttatttcaat tgaaaatctt ttatcatttc
    20461 atacgtgtgc ataattaaaa aggtgaaacc tcttattttg agatttcacc ttttttattg
    20521 ttaatttaaa tttaatttta atgattattt tgtttcacgg tgtaaagttt gtttgttttc
    20581 acgtgaacag aatttcttca tttcaacacg ttctggatta tttcttttgt tcttagttgt
    20641 aatgtagttt ctgtcaccac attccgtaca agctaaagtt acgtttacgc gcatggttat
    20701 tcctccttat catttctaaa tacgacttta ttattatacc aacatctata atatttgcaa
    20761 agttaaaatt ttacaaccat gcgctacaca atcgtcaggt taaattttat ttgtctttgt
    20821 ctttattttt atcattttta cctaactgct taaagtagta gttaattaca tctctaccta
    20881 agtctccacc tgttaaccat ggtggtggta caggctgatt tgtatataca attgaaaacg
    20941 ctaattttgg atcatcaatt ggcgcgtatc ctatataagt agagttaact cttggctctc
    21001 cgttttggaa cacttctgcg gtacccgttt taccagcagt aggtactact gtatctttaa
    21061 aactaacata tccagtacca tctttatcat taaatgccat tttgaatcct tcttgaattt
    21121 gtttgatttc cttttcagta ttattaacct tgttcaagac agtgccatta attttcttct
    21181 tgagtggacc aacctcatct ttattagttg attcatgaat cgttaatcca atgtgtggct
    21241 gtattctata accatcattc gctatagttg aaacatattg tgataattgt aatggtgtat
    21301 aggtatcata ttgaccaatt gataaatcta gataattacc tggattattt gttaatggtt
    21361 cgatttgacc tcttgtttca tttggtaaat ctatccctgt tttcacacct aagcctactt
    21421 gatttaatcc tcttcttagc ttttgggcag gtgaacttat gtctgaaggt aaagccatac
    21481 cagaataata agggtctccc gctaatttta atgctgtttt aaacatatat acgtttgatg
    21541 aatgcatcaa agcttgctta tcattaatag ttacatgccc gtttttattg aagtatgatc
    21601 gttttgtcaa accaccttgg aaatgtaatg gttcatcgac cattgtttct ccaactttga
    21661 tagctttatt ctgataaccg gctaataatg ttccaccttt tacagaagat ccaaccgcaa
    21721 attgagaagt aaacgtacca atgtcataat cagtcatttt accactctta ttaatctgct
    21781 ttccggcaag cgcaagaatg tctccatttt taggattttg tacaaccatc attgcattat
    21841 ccatatcttt ggcaccttga ctgcgaagct tcttaatttg tttatctaat aatgcttcta
    21901 cttctttttg aagatctata tcgatcgtta atttcaaatc ttgaccgcga gcgccaggat
    21961 ttaacacttc tgaagatgta actttaccag atttgtccgt tgtgtatttc atttctttct
    22021 tcttaccacg caatacatct tcatattgat attctaggta agattttcca acacgatcat
    22081 tgcgtgaata tcctttggat aagtaatgtt ctgtcaattc ttttggaata ccttcagcag
    22141 gtgtcgatac atctccgaat atacctctta aagtatcgcc atatggatat tttctatccc
    22201 aatccataga cgtgttaaca cctggtaatt tggaaagttg ctgagaaact gctgcatact
    22261 ctttttcact gacatcttca ttttttatca tttgtggatc taaaactgtt cctgcattca
    22321 tctctcgaaa aatagctaaa acttgtaaat ctttagaaga caattcatct aattgtgatt
    22381 ttccgatttt cgataacagt tgtttatcat attgatcttg tttaatactt ccatctgcta
    22441 acatagcttg ttcttttgtc atcattgctt ttgctttttt aggatgcaac tgaatccaga
    22501 aatctttctt atcacgttct gtaattttct tagtatccat cttgattagc tttgataact
    22561 tttcagccgt atccaacatt tccgattgtg ttgtttttcg acccctagta tatgtaatag
    22621 ccattttaga agcattatca actaaaactt tcccatttct gtctaaaata cgacctcttg
    22681 gcacagactc attcactgta atgttttcat cattttttat aatttgttta taatgtgagc
    22741 cttgtgcgat ttgtaaataa cctaaacgta gtactagtac tgcaaaaata aatataatca
    22801 caccaaatat aaagttaatt ctcttgttaa ttgtattttg aacgatttca tcatttgatt
    22861 tttcttttag tctttttaac aaaactacct acctctattc aaagtctttc actttaaatc
    22921 atatatgaat ttagaaatta tttctatctt tttgacaaaa aaataacggt ctcatttaag
    22981 agaccgaaca agtaatcata ctttattttg ttgcattata taattcgtca actttttccc
    23041 agttaactac attccaaaat gcgccaatgt agtcagggcg tttgttttga tattttaggt
    23101 aataagcgtg ttcccatacg tctaaaccta aaataggtgt tttaccctca gttaatggat
    23161 tatcttggtt tggtgtagtc acaatttcta actggccatt gtttacgact aaccaagccc
    23221 aacctgaacc aaagcgtgca gctgctttgt cagcaaattc ttttttaaat tcttctaaag
    23281 aaccccattg ttctttaatt ttttctacta cagtaccttt ttcttctgag tttggtgaaa
    23341 gtaactccca gaataatgaa tggtttaaat gtccaccgcc attattacgt acagcagttt
    23401 ggatgttagc tggtacactg tctaaattag caacaatttc ttcaatagat ttagattcta
    23461 aatctgtacc ttctactgca gcatttaatt tcgtaacata cgtgttatga tgtctgtcat
    23521 ggtgaatttc catagtttct ttgtcaaaat gtggttctaa tgcatcaaat gcgtatggta
    23581 attttggtaa ttcaaaagcc ataaataatc atcctcctaa aatgtctgta agtaaataat
    23641 aacaagcagt gactggttca acaaagaatt tgcttaaatt ctactactta ttattttctc
    23701 tactcattta atataactca aatcaaaaat aattaaacat tttgtatata aaaagttaac
    23761 agatttgcca taaaatcata cgaacggagt atgaaatgaa cctttatctt ctataatata
    23821 aagaaatgag atttatgcat acatctgacc aaatgtgcat aaatctcatt tctttatatt
    23881 aatcttggca agactcacat acaccataaa cttcaagttt gtgtttgtga atattaacac
    23941 caggtagtga taattttatc tgatctattg gacaataatc tattaccttt gtatctccac
    24001 acttttcaca gataaaatga tgatgatgat ggtttgtaca agcgattcta aacttcattt
    24061 caccatcaag ttctgtattt tcaataattc ctaaatcttt aaataagtgc aggtttctat
    24121 atattgtgtc gaatgaaatt ccaggataat tttcatccat aacttgttgt atatactttg
    24181 cgtttatata cttatcttct tcgacaaaaa tatctaacat atctttacgt ttatctgtat
    24241 attttaaacc gttctctttt aaaattttaa tagcatcatt tgtattcatt gatattagct
    24301 ccctttttaa acttcattcg cattttctga taagccattg taatcataag taaaataaca
    24361 agtagaacta caattacacc acccggagaa atgtccatat agaaagctag gactaagcct
    24421 aatattactg ataattcacc taaaaataca cttagtaata tcaattgctt aaaacttttt
    24481 gttattcgca tacttattgc aattggtaac gtgattaacg cacttactaa cagtatccct
    24541 acaacacgca ttgaggcaga aataaccatc gctacaataa caataaataa aaattgaatc
    24601 catttaggaa tgccaatgac tttactatat tcctcatcaa atgacaatat aaataattct
    24661 ttataaaaca atgtaataaa cagaacaact atgatggcaa tgacaataat cgttgttaaa
    24721 tcacttatat tcactgcgct tattgagcca aatagcaatc caacaatttc ttgattgaac
    24781 ccatcagcta atgaaatgaa gattgcactc aaggcgatac cagcactcat tataattgga
    24841 atagcaattt cttggtaagc agtgtatgac gttcttaatt tttcaattag aagcgcacct
    24901 actattgcga ataagattcc aaaccacatt ggattaataa atactagtgt tggcataata
    24961 gtaagtaaaa acataccgaa agatatacca cctaaagtta catgacttag agcatcagct
    25021 ataagtgata gtcgtctaac aacgataaaa gcaccgatta gaggcgcaat aaaacctatc
    25081 aagataccac taattaaaga gtacctcata aaatcaaaat tcaataatgc atctatcaat
    25141 tgtgacacgc ctttccattt taaataaact caaatcttta ttaattacaa cattctcgat
    25201 tatgctgatg atcgacaaaa cgtacaggat gtccataaat ttttgaaatt tcaacttcat
    25261 caagtgattt aaactcatca gttgtaccat ggaaatgcaa atgcttattt aaacatgcta
    25321 cttcagtagc agtatctgct acaacaccga tatcatgagt aactaagata atggtgatac
    25381 cttcttgttt taattgatct aaagtattat aaaattcact tacatgtttt gcatcaatac
    25441 cattcgttgg ttcatcaagt actaatactg caggttctga aatcaatgct cgagcaatca
    25501 ttacacgttg ttgttgacca cctgataatt ctgctatatt tttatgaatt aaatcactta
    25561 tattcagtct ttctagtact ttaatcactt tttcattatc tttgctatta aatgtttgga
    25621 aaagacgttt tgtctttgtt aatccgctta aaacaacttc tttaacactt gctgggaaac
    25681 ctgaattaaa ggcatttgct ttttgtgata catagcttaa tttaattgat gttttcttat
    25741 ttttaaaatc aataccttca acaaaaatct caccactttg taaaggtaat aaccctagaa
    25801 tcaacttcaa taatgttgat ttaccagcac catttggtcc aacaattgct aaaaattcac
    25861 ctttatttat tttaatgttt atattttcta acactttttt atgatcatag tagtaattga
    25921 catttttcaa ttcaaagact ggtgtcatcg tattctcacc tcgcattcaa ctatacaact
    25981 cctagtaaca tatgtaaaca gtaatgttta cgactcaaaa ttagacaaaa taaagagata
    26041 tgcccccttc aagttttatt tatcgcattt cttgaagaga gcattatcat tttattgttg
    26101 cataacctta ttttttaatt ctgggtcaaa ttgctgttgt tttaacattt caatttcaag
    26161 tttatatggc ggttttttat ttttcttatc ttcaccaaca taaggtgttt ctaagatttt
    26221 cggaatatct ttaaaactat catgatgcac aatgtaattt aatgcatcaa aaccaatgta
    26281 accgaagcca atattttcat gtcggtcttt ttgagcgcca cggtcatttt tagaatcatt
    26341 gacatgaaca actttgattc tgtcgactcc aatgatttta tcaaattcat ttaatacgcc
    26401 atcaaagtcc tctttaacat tatatccagc atcatgcgta tgacatgtat caaaacatac
    26461 tgataaacgt tcgttattat gaactccatc aataatacgt gctaactctt caaatgagcg
    26521 accaatctct gtacctttac ctgccatcgt ttcaagcgca atacgtacat tattgtcatt
    26581 cgttaaaact tcatttaatc cttcaataat cttattaatt ccggcatcaa caccagctcc
    26641 aacatgcgca cctggatgta atacaatatc tttagcccct atagcttgcg ttctttcaat
    26701 ttcttgttgc aagaaatcta caccaagatt aaacgtttct ggtttggttg tatttgcaat
    26761 attaatgatg tatggtgcat gaacaacaat attagataaa ccatattttt ccatcacttc
    26821 atgaccttta gttatattta aatcttcaat acttttacgg cgcgtgtttt gaggtgcacc
    26881 agtataaatc ataaatgttg tttcaccata ttcatgcgct tctatagcag aaccttctaa
    26941 catcttttta ccactcattg aaacatgtga tcctaataac ataaaacaca cctaaccttt
    27001 tttgttttgc ttacgttttt gtctattttg ctgcttacta aattgcttac gctcttgacg
    27061 tttcattttt tcaacttctt gtttaaattt cttcttataa cctggtttaa ctttgttttt
    27121 aattttactt cgaactttgt tcttcacttg attagttaaa tggtcatctt tgcgcattct
    27181 tgcttgacgc tgattgtgcg ctttaacttc ttttaactca ccatctttaa tatcaacagt
    27241 attgaataca aaaccgcgat cttctattaa tgaaatattg tgttcttcat caggactata
    27301 aagcgtaatt gctacacctt tataattccc acgaccagtt cgtccgactc tatgcgtaaa
    27361 gaagtcaata tcatttggca catcaaaatt gatgacatga ctaacacctt caatatcaat
    27421 accacgagat gctaaatcgc tggcaataac gtattggaat tctaaattac gtatacgttt
    27481 catttgttgt ttacgttcac gtggcgttaa gccaccatga atcataccaa ctttaatacc
    27541 agcttcattt agtgaacgtg ctaaatcatt tgcattatct ctactattac agaaaataat
    27601 acataagtat ggatttagta tatcaattaa atttaaagtc ttttcaactt tagctgcacc
    27661 tttagtaggt attaaataga attcgatgtt ctttttattt tgttttttac tgtcgacagc
    27721 tacatattct ggatgactta aatatttatt taaaaatggt tgtaactgtt gtgggattgt
    27781 agcactaaac accgcaatat ttgcattatc ttccaatctt gcagcaatgt aatctacatc
    27841 ttcaattaat cctaagtcaa tcataagatc cgcttcatca ataactaaat atgatgctaa
    27901 gtgcacatgt aaatgtcccg ttttagctaa gtcattaatt ctagtagggg tgcctataat
    27961 caattgtggt tgtgcattac aacgttgtct atctttctct atatctgtac caccaataaa
    28021 aactttaact gaaacaccag ctttaaattg gcttaaatgg ttcgctgcat cgtatagttg
    28081 ttgtgcaagt tctcttgttg gtgcaactac gattgcttgt ggttctttta tttcactatc
    28141 aattaactgc attaatggta ataaaaatgc atgagatttc cctgtacccg tttgagattg
    28201 accaattaaa tttgttctct ttagtattct tggaataatt cgattctgaa tttcagttgg
    28261 tttttcaaaa ttaaggtctt tcacagcgtc aattaaacta gattctagat taaattgttc
    28321 gaatggatgt tttgccatga tttggcctcc ttatatgttc atctttacta ttataaagta
    28381 taaatgtgct attttaaata aaaatattaa aacctcgaaa tttcaaaatt tagaaatccc
    28441 gagattttag tttaaatata ttgaaatgga tctgtattaa ttgtagatgc ttcaacatct
    28501 aaatttattt tttctgtatt gaaccaattc attagtaacg cttttaaacc ttctttcatc
    28561 acatattcgc tgtaatgatt aatatcaatt aaattcacac catgaatttt agcatctaag
    28621 gcatcatgat gtttaatatc acctgtaaca aagacatctg cgccttgttg gacagcttga
    28681 tattcatatc caatacctga accaccaata attgcaatac gtttaatttt ctgattagac
    28741 tcaccaacaa aacggacact tgggatattt aatttagatt taatatcagc tgcgaaatct
    28801 tccaatgtca tttgattatc cacttctgcc ataacgccaa gtccataaag ggatgtttgt
    28861 tttatctcaa taaaatcaaa taccggtgtt tcatatggat ggtattgttt aattaattgc
    28921 tcagcccttg acttttgata tgcatctatc ataaattcaa ttttaacttc atctacatat
    28981 tcaattttat caatttgtcc tattgttgga ttagcttcac caactggttt gaattgccct
    29041 cttccttcac tttcaaagaa acaatattca taattacctt cttgcgctaa tccattttca
    29101 ctaagcttat ctttaaatgg tccaacatta tccttaggta tatatgtttg aactttatag
    29161 tatacatctt gttgattatt tattattgaa atgttcttca aacccatcgc cttcgccaac
    29221 atcatattga caccatacgg atttacatct aaatttgtat gcatcgctat taaattaatg
    29281 tcatgttgaa ttagttttct aatgatcaaa ccataaccat tagcttttaa tgatgttacg
    29341 cctttaaaga ttagaggatg atgactaata atagtattat aacctttttc gattgcttca
    29401 tttactactt ccaacgtaca gtctaatgct gttaaaacac cagtaacttc aacatcttca
    29461 tcacctatta acaatcctac attatcccaa gattcagcag tactaaatgg cacatgatga
    29521 tctaacaatg tcattaaatc agctattttc attactataa caccctttca attacagcaa
    29581 tttcgtcatt aatttgagct aaacgttgat gatgttgttc agtattgagt ttcgatttaa
    29641 tatgataaag tgcttctaac tctctttgcc atttttttat aaaatattca tttttgttgt
    29701 tcagcaattt tggtccgaat ttcaattcat cagatgatag ctctattaat tctgtagaat
    29761 attctgctac aacaatttca taaatatggc ctttttcttc cattattatt tcatcaatta
    29821 tttcataatt caattgttgt aatgtttgtc ttaaattttc agtttggata ttactttgta
    29881 aaatcaacct tggatgttga cttaacttat cttgcccatc ttttaaaatt ttagcaataa
    29941 gtggtccgcc cataccacaa attgtgatat tatcgattac gtcctcaggt tgaataacac
    30001 ttaagccatc ccctaaacgt acatcaattc tatctactaa ttggtttgca gctacatttt
    30061 tcacagcagc ttgaaaaggg ccttgaataa cttctccagc aataccgcat tcgcataaat
    30121 ggttttgaat tgcatagatt ggcaaataag catgatctga gccaatatcc gcgattgtac
    30181 cttgttttaa aaatcgactt accgtcgtta atcggttatt taacgaaatc attttattct
    30241 cctttatgaa aaaagaggtt gaacattatt caatgtacca acctcactca agttaaatct
    30301 taatctaaaa ataaataagc gcttattcat gtcttggtat caatcattaa aattgatata
    30361 aattaatcca taaagtcttt caaacgttta ctacgacttg gatgtcttaa ttttctaagt
    30421 gcttttgctt caatttgtcg aatacgttca cgtgtaacac cgaaaacttt accaacttct
    30481 tcaagtgttc ttgttctgcc gtcatcaaga ccaaatctta atcgtaatac attttcttct
    30541 ctatcagtta atgtatcaag cacatcttct aattgctctt ttaataattc ataagcagca
    30601 tgatctgaag gactttgtgc ttcctgatcc tcaataaagt ctcctaaatg actatcatct
    30661 tcttcaccaa ttggtgtttc taatgaaaca ggttcttgcg caatttttaa aatttcacga
    30721 actttttctg ctggtaaatc catttcttca ccaatttctt ctggtgctgg atctcgacct
    30781 aagtcctgta ataattgacg ttgaacacga attaatttat taattgtttc taccatatgc
    30841 acagggatac gaatcgtacg tgcttggtca gcaattgcac gagtgattgc ttgtctaatc
    30901 caccatgttg catatgttga aaacttaaat cctttgttaa agtcaaattt ttcaacagct
    30961 ttaataagac ccatattacc ttcttggatt aaatcaagga ataacatacc acgacctacg
    31021 tatcttttag caatacttac aactaaacgt aagttcgctt ctgcaagtct tgattttgct
    31081 acttcatcac cttgttcaat acgtttggct aattcgattt cttcttgtgc acttaataag
    31141 ttaacacgcc caatttcttt aaggtacata cgaactgggt catttatttt aacacctgga
    31201 ggggcactaa gatcacttgg attcagtttc tcgtcagtat ctgaactatc tttttcatta
    31261 actagtgaaa tatcattatc atttaattga tcaaagaaat catccatttg atcagagtcg
    31321 atatcaaaat tctgaagttt ttcagcaatt tcttcatgac ttaaatgacc ctctttttta
    31381 cctttttcaa ttaattgctt cttaacatct tctaatgtta atgtcggatc aattgtttgt
    31441 tttttaattt taactgtgtt atcagacatg aaacggcctc ccgattttaa atatgaacat
    31501 tcgaaattta ttcaatattg ctattttaaa ctaaattctt aattaattca tccatatttt
    31561 taattttatt ttacaaattg gaactaatcc caatatttat ttttcaatag tgttgtttaa
    31621 atgcggtaac tatcatttac ccttatacat tttgacataa tcattattcg tattattctt
    31681 taaaatcaca tgctacatgc gttctttatt cttagcaaca atttgctgta aatagtattt
    31741 ttgtaattct acatcgccaa tccttgtagc ttcccttaat ttatgattca atgactcaat
    31801 tgtttcttgt cctttttcat taataacatt gacataatca tcaatttcat tttcatatgg
    31861 ttcgtcattc aaattatatt gttctaagct aattagtgtt tctctcaact catttgaatt
    31921 aacatactgc acagcatcac tgatattata ttgatcattt tccgcataaa aatcatgtaa
    31981 gacttcgaat acatatttaa aatgctgatt tgtgaagtta tccttatcaa cactttcata
    32041 ataatttaaa aatgtatctt tatctctcat taaatgtttt aaaaatgctc gctccgcttt
    32101 ttcttgacgg ctcaaattgt caaattgtgc cataccaatt ggctcaggtt caatgtaacc
    32161 gccatactca tcttctggat aataattggc tggtgcttga ttgaattgta tttcgttagc
    32221 taattgctca ggactaacat tgaaaaatgg tgcaacatca tttaaagcct tttgttgcaa
    32281 aatcgatgat ttcataagcg aaatatcatg acttagttct ttcaaataac gttcatatga
    32341 aaggtcatta tgtgcaattt catcttttaa tatactcact ttataatgtg caaatgactt
    32401 tttgtcattt tttacaaaag cagtaaatgc atcgttgcca tacttaccaa tgtattcatc
    32461 cggatccatg cctgatggca attgtataac aaatacattt agcccttgct gtaacaaatt
    32521 ttgacctgtt ttaagtgttg cttcactacc cgcaaaatcc ccatcaaaca ttaatgttat
    32581 atttgatgtt aactttcgta taaaagtaat atgttcatct gacaactgtg tacccattgt
    32641 tgcaacaacg tttttcaagc cagcagtatc agattttata acatccataa aaccttctag
    32701 taatacgatt tcatctaatt ttctaattga tttacgcgct ttatctaagt tgtataacaa
    32761 ctttcttttt tgaaagatag gtgtttcagg actatttaag tattttggtt cttgaccggt
    32821 atatgttcga cctgaatatc caacaattct tccttgcgca tttttcaaag gaaacataat
    32881 acgatttcga aatctatcgt aataactgaa attttcttcg ttacgtgata ataatccggc
    32941 ttcatatgct aattcaatat cgtaaccctt tttttgaaga aaatcatgac aaaaatgtga
    33001 gctatcgggt gcaaagccaa tgcctcgctc tttaataagc gcatctgtaa aaccacgttc
    33061 ttgtaagtac gttaatgctt gttcgccttc gactgtcttt gttaaagcgt aataataaaa
    33121 ttcttgtatt aactcatgca tttcaatcat ttgtaaatca tcagaagcaa tttgaacatt
    33181 tgagttagat tgtgttgcct caatatctac agcaacatta actctatcac ctaattcttt
    33241 aaccgcttca acaaatgata tgtctttaat ttcttgagta aattgaaaaa cattgccacc
    33301 ttttttacaa ccaaaacaat gacaaatttg tttatcttca gaaactgtaa atgaaggtgt
    33361 cttttcatca tgaaaaggac acaaacctat ataattgcgt cctctctttt ctaattttac
    33421 atattcactt accaagtcta aaatgtcggt tttatctttt atttcattaa tgatcgattg
    33481 atctattcgc aaattaaatc acctattaag cattagttat actattatac ctaattttcg
    33541 acaaaaatga aatcatttcg atttattttg ttcaatataa tggattatat cgtttgctgt
    33601 ttcttcgatt gctttttgag aaacatcaat gacaggacat ccaatttcac ttacgatttc
    33661 ttcaaagtaa ttcaattctt cttgaattcg tgcttctgtt gcatatcgag ctgtgtcacc
    33721 tagtcctaat tgttttagtc gctcttttct aatgcgattt aatttttctt cacttatttt
    33781 aagtgcgata catttctttg gattaatatc atataagcca tctggcggtg tcacttctgg
    33841 tacaatcggt acattcataa ctttgtaact cttatgcgct aaatactgag ataatggtgt
    33901 ctttgaagtt ctcgaaatac caagtaaaac aatatcagct ttaggtaatc ctttaggatc
    33961 tttaccatca tcatatttaa ctgcaaactc tatcgcatca attttcttga aatatgcatc
    34021 atctaatcta tgaacgatac ctggctcatt ataaggtttt tcttcaaccg aagcagataa
    34081 taaatccatt aatggcccca tgatatcgac agacttcaat tggaattctg ctactttctc
    34141 actcatatat tgcttcattt caggtttaat aagtgtataa acaatgatag catttgtatc
    34201 ttttgcaact tgaatcactt catcaacatc ttcaaaagat tcaatatatg gatatcttaa
    34261 taattcattt ttacattgct taggattgaa ttgtgaaata cctgccctag caactaactc
    34321 tgccgtttca cctatagaat ctgaagctac gataatttta attttttcca ttacttctca
    34381 cctattcttt atataatgct actaataact tagctattgt tgttttggaa attcttccaa
    34441 ttacttcata cttttgatta tctttttttc ttacaattgg aatcgaatca atttcttttt
    34501 caatcattct atctgctgcg tatatgacta attcgctttc ctctaaataa gtgacattag
    34561 gcatacgtgt catatttaca ctgataggta ctttatgaat atctgctcca atcattgaag
    34621 ctcttaataa atcttttctt gaacacacac caacaaaatc gttatcttca ttaataataa
    34681 ataatgtact tacatcttct aaaaaaattg tacaaatagc gtcataaact gttgtattct
    34741 ctcttagcac aacaggttga gacatatagt ccttaacctc aaattgtcga agtttttcat
    34801 taaaaaattt gccttttgat ttacctgaat aataatatcc aactcgggga cgcgcttcta
    34861 aaaaacctga cattgttaat atcgctaaat ctggtctaag cgttgctctt gttaaattca
    34921 acttatctgc tatttgttca ccagtaatgg gtcctttagt tttaacaatt tcgatgattc
    34981 gttcttgtct ttgactgagt tctataggtc ttcacccctt ttttattcac ctattattat
    35041 accgatattt ctttcatgtc acaaacacat taattttact tgcctttaaa taatctatca
    35101 attagaataa acgtacagcg agttaaggat agtgtaagct taacaataag attggcgcaa
    35161 cgaatcattt taaaataaaa gcgagtgact acactaattt gggtggaacc gcgggttaaa
    35221 tacgtaactt attttgtatt atatacaatt taaaatatta actcgtccca tggaagtaaa
    35281 gcatatttac tatgctgtta ttttccatgg ggcgttttca tgtatgagga gaggtaatta
    35341 tggcaaaaga tatggataca attgtttcat tagcaaaaca cagaggtttt gtgttccctg
    35401 gtagtgatat ttacggtggt ttatcaaaca catgggatta tggtccttta ggtgttgaat
    35461 taaagaataa tgttaaaaaa gcttggtggc aaaaattcat tacacaatca ccgtttaacg
    35521 ttggtatcga tgctgcaatc ttaatgaatc caaaagtatg ggaagcttca ggacacttaa
    35581 acaacttcaa cgacccaatg attgataata aagatagtaa aattcgatat cgcgctgata
    35641 aattaattga agattatatg caagatgtta aaggtgatga aaacttcatt gccgatggtt
    35701 taagttttga acaaatgaaa aaaattattg acgatgaagg tattgtttgt cctgtaagta
    35761 aaactgctaa ctggactgaa attcgccaat tcaatttaat gtttaaaaca ttccaaggtg
    35821 taactgaaga ttctacaaat gaaattttct tacgtcctga aacagcacaa ggtatttttg
    35881 taaactataa aaacgtgcaa cgttcaatgc gtaaaaaatt accatttggt atcggtcaaa
    35941 ttggtaaatc attccgtaat gaaatcactc caggtaactt cattttcaga acaagagaat
    36001 ttgaacaaat ggaacttgaa ttcttctgta aacctggaga agaaatcgaa tggcaaaatt
    36061 attggaaaac ttttgcaagt gactggttaa caagcttaaa tatgagcagt gaaaatatgc
    36121 gtttacgtga tcatgatgaa gatgaattat ctcattactc aaatgcaaca actgatattg
    36181 aatataaatt cccatttggt tggggtgagt tatggggtat cgcaagtcgt acagacttcg
    36241 acttacgtaa acatgctgaa cactctggtg aagatttcag ataccatgat ccagaaacga
    36301 acgaaaaata tattccatat tgtatcgagc catcacttgg tgcagatcgt gtaacattag
    36361 ctttcttatg tgatgcatat gatgaagaag gcgttgaagg tagtaaagat gcacgtacag
    36421 ttttacactt ccatcctgca ttagcaccat ataaagcagc gattttacct ttaagtaaga
    36481 aattatctgg cgaagcgatt aagatttttg agcaattaag ttctaaattc tcaatcgatt
    36541 tcgatgaatc acaatctatc ggtaaaagat accgtcgtca agatgaaatc ggtacacctt
    36601 attgtgtaac attcgacttt gattcattag aagataatca agttacagta cgtgacagag
    36661 attcaatgga acaagttcgt atgccaatct cagagttaga agctttctta actgaaaaaa
    36721 caaaattcta attaaaaaga ctttccacca tatgtggtgg ctatataatt tataaaaaga
    36781 gatactggtt agtaaaattg ccagtatctc ttttctattc aaaaaggtat tcaaataaac
    36841 acatctatgt ggctacttga atacctttta ttattttatg ttattgttcc aatcttttta
    36901 attggttgat tagtttctga cttttaaaaa acatacctgc atattcacga tataacatta
    36961 aaatgatatc tgacatttca ccaataattt cttgatggat attcaatgaa ttcattttat
    37021 ctatcggtaa tttttgtaat acatctaata aatatagtgt tttattcgat aatataactg
    37081 catgtacatc tttagaagcc tcttgccttg aaatcgcacc gtcaaactta aaactataac
    37141 ctattaaatc tgcttgtgtg tcattaccac tcacagcaca gcgattaaat gatgcagtaa
    37201 aaccaaatcg tttcatgcac tttaacataa ctacgactga cattaactgt gcagatgtac
    37261 ctgattctat tttttcaaga acaaattgta ataattgata gttatatggt gcaatgtcac
    37321 cttcatccat tgagcgctca atagtttcag ctgccagagc ggcataactg cttacgtaaa
    37381 ggtccatttg taatttataa tgttgactaa taacatctac agaatttaac gttcccatac
    37441 ctcgccactg attgtaaata aacaaaccat aaacaaacaa ttgcgtttgc gcttgtaaac
    37501 ccgtcttaac ttttttagca cgccttgcca taagtggtac ttttgcacca tgctcattta
    37561 aaatcgtgat aattttatca gattcaccat aatcaactgc tttgatgata atcccttttt
    37621 ggcgcattaa caattatctt caccactttt aagattaatc ttggtcttca acataaccaa
    37681 tttggcgaat aaagttaact ttgtttcgcc agtctctttg aactttgacc cataattcta
    37741 agtaaacttt agagcctaga agcatttcta tatcacgtct cgcacgtttt cctacttctt
    37801 ttaacttttt accgcctttt ccaatgacaa ttcctttttg cgaatctctt tcaacatata
    37861 tagttgcttc gatatgaaca cgatcttcgc tttctttaac catacggtcc acattaacac
    37921 caatcgcatg agggatttct tcacttgtaa gatgaaggat tttttcacga atgatttcac
    37981 ccactacaaa ttgttcagga tggtctgaaa tttgatcatc tggataatat ttaggtcctt
    38041 cgggtaaata cgtctttaaa acatcaataa aatgatcgac atttagccct tctaatgctg
    38101 aaataggtac aatctctgta aagtccatat aactttgata ttcttcaatc tttggcatta
    38161 attcatctgg atgcactaaa tctattttat ttaatactaa aaatactggt gtcttaacat
    38221 ttttcaacat ttctataata tattcatcgc ctcgtccaat ttcctcattg gcattaacca
    38281 taaacatgat tgcatctatc tcagataatg tatttttagc gactttcatc atatagtcac
    38341 ctaatttgtg tttaggttta tgaatacctg gcgtatcaat gaatataatt tgcgcgtcat
    38401 ctcttgtcat aacaccttga attttatttc tagttgtttg agctttatcg gacatgattg
    38461 ctattttatg gccgatcact ctattaacaa atgttgactt tcctacattt ggtctaccta
    38521 taattgaaac aaatcctgat ttatgttctg tcatttattc taaatccttt cctgaaaatc
    38581 caaatggtag taactctgcg actgtcatca taaccatatc tcctttatga tttgtcatat
    38641 acacaggcat atcatcatca cataattcct tcaaaacttg acgacatgca ccacaaggtg
    38701 atgacggttt atctgcatct acggttacag ttattgattc aaaatcacct ggtctgtatc
    38761 cttgagaaat tgccggtacc aaactagctc gttcagcaca tatcgataat ggataagaag
    38821 cattttctac attggtacca taaaaagttc taccgtcttt cgtttttaaa taagccccta
    38881 ctttaaattg actgtatggc gaatatgatt cttgttgtgc ttttctaact tcttgaaaat
    38941 aatgaggttg ataactcata tatgcctccc taaaataacg ctataaaatg tggtaaaaat
    39001 actattaaac ctataataaa tgctaatatt gaaactataa gtacactaaa agccgcaata
    39061 tctttagcgt atttagctaa atcatgatat tcaacggtca ctaaatcgac aacatattca
    39121 atagcagtgt ttaaagcttc aacagtgaga actaatgcaa tagcaatgag tataaatatc
    39181 cactcaatcc gattaatatt tagtacgaga ccaaagacaa tagcaacaat cattgcaaac
    39241 acatgtaaaa gaaatttata gtctttttga attaagattt tcagcccatc aagtgcatat
    39301 ttaaaccttt tcataattag tctcgtgtta atccatatgc gtttaatatt gtatcttgtc
    39361 gaccaaacat ttccttttca tccgcttcag tcatatgatc ataacctaat agatgcaaaa
    39421 atccatgtaa tgctaaaaat cctaattctc gttcaaaaga atgtccgtaa ttgtttgctt
    39481 gttcttgcgc tacatccgta cagataatta tatcccctaa aacacgtggt atatcaagac
    39541 cactaaaatc aatctctggc tcatcttctt ctaaagcaaa tgagattaca tctgtaactt
    39601 tatctttatc tctatatgtt cgattaattt cttgtatttc ttgtttatct acaaatgtaa
    39661 cagaaagctc agcatcgtct tctatatgct cttctttttt agcaaattct aataaatctt
    39721 caatttgttt ataccaagca tctttaacta agcctgtgtg atcgctaaaa tctatcgtaa
    39781 acatttaatt ctctccttca taatgttcaa tgatcttact taccaatgga tgtctgacta
    39841 catcgctctg atctaatttc aatatactta tacctttaac gttgtgtaac ctactgaccg
    39901 cttccttaag tccactttta acacctttag gtaaatcgat ttgagtttgg tcaccagtaa
    39961 ctaccatttt tgagccaaaa cctagtcttg ttaaaaacat tttcatttgc gcatgtgtcg
    40021 tattctgcgc ctcatcaaga attacaaatg catcttctaa tgttcgtccg cgcatatatg
    40081 caagtggcgc tatttcgata atgcctcttt caataaatcg ctctgtttgt tcacgcccaa
    40141 gaacagtata tagaccatca tataaaggtc ttaaatatgg atctaccttt tctttcaaat
    40201 ctcctggtaa aaatccaagt gactctcctg cttcaacagc aggtcttgtt aatacaatac
    40261 gtttaacagc acctttacgg agttgctttg ctgcataaac tacagctaag aatgtcttac
    40321 ctgtaccagc aggacctata ccaaatacta aatcattatt tttcatggca ttaacatata
    40381 tacgttgccc catcgttttc gcacgaatcg tcttaccaaa tgcatcttta gttatctctt
    40441 catcatataa atctaacaga tgttgaattg tgttattatg cgccatttta atagctgctt
    40501 caacatcttt aattgtaata ttattaccta aatcaataac cttcagcaaa ttgattaata
    40561 ctgattccgc tttttctacg ttttctattt ttgtaccttt aacggcaact tcttgtcctc
    40621 ttgcatggat gacaacatcg aaactctctt caattgcttt taaatgttca tcattatttc
    40681 caattaaagc ttgagattgg ttcatatcgt ctatttgtat aattccaggc atacacgcgc
    40741 tccttttcat atatcattta tattcattct atgatgcttt aatatctact taattatatc
    40801 atgcaattga ttgatttaaa catctttgca ttgacttact catttttctt taatttaaaa
    40861 taatcattta tacttttcac cttgtcataa aagcagattc aattttagac taatcaattt
    40921 tttgctgtaa tttaaataac taatatttct cgaaaaattc tctttgtttt ttaatttttc
    40981 ttaacgtaaa aaaacaagca actaccatta cgatagtgct tgttttcaaa aaattttata
    41041 attgttttgg tttagctaaa atttctgacc atatcatacc attgattact tcatctttat
    41101 caaattggaa cgctgattta gtcaaatctt tttcgacatt ttgagaatta agcagctgct
    41161 ttaatttcaa acgttttgta cgttcagata agtatttatc ctcaataata tctctagcac
    41221 gtttttccat tttagcaatt tgcttttctt tttctctatc aatgtcagta cgaatcgttt
    41281 taatatcatc tctaagtgat ttttctaatt gacgtctaat ttcgtcagaa ttatcttgtc
    41341 tagaaggtct atgtttctgc tccaccggtt ctgctttagg cactgtgatt ggtttctctg
    41401 tcatcggttt tgtttcttgt tgttttttag gtgccatagg ttctacaaca ggtttcgatt
    41461 taggctcttc ctttggaagt tcatcgaata aaggtggtaa cgtatcatca tattttcgtt
    41521 tcgatgattt cttttcttct tcatttaatt cttcacttat ttctttaaac gttcgctcaa
    41581 tttcttcaaa aaagccacct ttttttggtt cattatcggt agatgttttt tgaggtggct
    41641 tttgattttg tctatcttta tgactatttt cgcgcatagt agtaatgata gaaatgatca
    41701 ctgatatgac aaaaattaga ataccgacac tcatttaatc acctctcgac ttaatgttca
    41761 ggtgactcat catcactttg atcagttcgt ttattaattg catttctcat gcctgtatca
    41821 gcttcgatat ttttcaaatt ataataatct ttaacactga tattacctga acgtaatgct
    41881 tcagccatag ctaatggtac ttcagattcg gcttcaacta ctttagcatg catttcttgt
    41941 acacgcgctt tcatttcttg ctcagttgct acagccatag ctctacgttc ttcagctttt
    42001 gcttgtgcaa tatttttgtc tgctaatgct tgttcagttt gtaagtctgc accaatattt
    42061 ttactaatat caacgtcagc aatatcaatt gataaaattt caaatgcagt acctgaatct
    42121 aaacctttgc ttaaaactgt tttagaaata ttatctgggt tttcaagtac ttctgtatga
    42181 tgcttactag aaccaattgt tgaaacgata ccttcaccaa cacgtgcgat gattgtttct
    42241 tcaccagcac caccaacaag tcgagcaata ttagctctaa ctgtgatacg agctttggct
    42301 ttcacttcaa taccgttcat tgctacacct gcgataaatg gtgtttcaat gactttagga
    42361 ttaacagaca tttgaaccgc ttctaatacg tcacgtcctg caaggtcaat tgcagcagca
    42421 cgttcgaaag gaagatcaat gtcagcacgt tgtgcagcaa tattagcgtc aacaactctg
    42481 tcaacatttc ctcctgctag ataatgcgat tctaattggt ttgttgttaa tgctagtcct
    42541 gctttgtgcg ctttaattaa tggcgctata acttttcttg gagatacacg acgtaaacgc
    42601 ataccaacca atgtacctat accaacatga acgccagctg ctaacgctga aatccataaa
    42661 ccaatgggta caaatgagaa taaaataagt aatgcaacta ctataataac tgctattacg
    42721 ataaaactta aactaaacat ggtatcgctc cttttaatta atctacttcc ctcacaacta
    42781 ctcttgttcc ttcaacttca aggattttta ccgttttatt gcgcaaaata aagttgccat
    42841 ctgaaacagc atcaatacgt tcattttcac aaaaaataat ccctgcaggt cgaagatctg
    42901 taactgtttg agcagtcttt cctacgaggt gcgagcggtt atcatgagaa ttgtaacctg
    42961 actcagaatt agttgaatct tttaagataa ctttatccaa aaacggaatc tttctgttga
    43021 aaatcttcac taatatcacc cattctacaa tcgttaaaat caaggcaacg ataacattcg
    43081 caagcataaa tagcaaatta tcaccgagcg ttgttatgct tatagttatc agtatcatgc
    43141 caataatacc aattactgca ccaactacaa ataattcaat tacaactaat ataacgccaa
    43201 ttgagaatat taagatagaa tgcatattga catttccttg gattagaaat cccaaaaata
    43261 aaataagtaa tgataatgtg gcgataatac cagctgcatt gattttttta gagtaaagtt
    43321 gatatacaaa tcctaagaat gtcaaacagg ttaatattaa cgtaaaaata ggttgaacaa
    43381 ttatattgct aacttgttca acccaagtat ctccagccgt tgattccaag atagttgtca
    43441 tttgtaaaaa attattataa ctcacaattt cacctcgccc tcacattaat tatacatgaa
    43501 attgaatata ctttatagtt ataagaacct atagaatatc aatttgatta tttataaact
    43561 atataacttt ttttaattaa aaattgacaa caaaaaaaca tcacatatta gtgtcagaca
    43621 ctaacttgtg atgtttgttt tatataatta atattcgtgt tgagggagtc aacagaggta
    43681 ttaattattt gaatttacgt ttacgtgcag cttctgattt ctttttacgt tttacgcttg
    43741 gtttttcgta aaattcacgt ttacgtactt cttggattgt tccactttta gaaactgaac
    43801 gtttaaatct acgtaacgca tcttcaagtg attcattttt acgtactact gttttagaca
    43861 tctgtatttc cctccctcca aatatcaacg gaactttata gtcaattgca cagtaaaact
//
LOCUS       DANRDI010000005        41821 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.005, whole genome shotgun sequence.
ACCESSION   DANRDI010000005 DANRDI010000000
VERSION     DANRDI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 41821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.005"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(160..1797)
                     /locus_tag="PZ377_000145"
     CDS             complement(160..1797)
                     /locus_tag="PZ377_000145"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDM6357038.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(1957..2835)
                     /gene="hchA"
                     /locus_tag="PZ377_000146"
     CDS             complement(1957..2835)
                     /gene="hchA"
                     /locus_tag="PZ377_000146"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDM6357039.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(3101..4288)
                     /locus_tag="PZ377_000147"
     CDS             complement(3101..4288)
                     /locus_tag="PZ377_000147"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDM6357040.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            4459..5634
                     /locus_tag="PZ377_000148"
     CDS             4459..5634
                     /locus_tag="PZ377_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDM6357041.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     EHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(5916..7100)
                     /gene="tuf"
                     /locus_tag="PZ377_000149"
     CDS             complement(5916..7100)
                     /gene="tuf"
                     /locus_tag="PZ377_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDM6357042.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(7317..9398)
                     /gene="fusA"
                     /locus_tag="PZ377_000150"
     CDS             complement(7317..9398)
                     /gene="fusA"
                     /locus_tag="PZ377_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDM6357043.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(9521..9991)
                     /gene="rpsG"
                     /locus_tag="PZ377_000151"
     CDS             complement(9521..9991)
                     /gene="rpsG"
                     /locus_tag="PZ377_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDM6357044.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(10057..10470)
                     /gene="rpsL"
                     /locus_tag="PZ377_000152"
     CDS             complement(10057..10470)
                     /gene="rpsL"
                     /locus_tag="PZ377_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDM6357045.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(10568..10822)
                     /locus_tag="PZ377_000153"
     CDS             complement(10568..10822)
                     /locus_tag="PZ377_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDM6357046.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(10959..14555)
                     /gene="rpoC"
                     /locus_tag="PZ377_000154"
     CDS             complement(10959..14555)
                     /gene="rpoC"
                     /locus_tag="PZ377_000154"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDM6357047.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPDAEVESQTEVTE"
     gene            complement(14719..18270)
                     /gene="rpoB"
                     /locus_tag="PZ377_000155"
     CDS             complement(14719..18270)
                     /gene="rpoB"
                     /locus_tag="PZ377_000155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDM6357048.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(18485..19093)
                     /locus_tag="PZ377_000156"
     CDS             complement(18485..19093)
                     /locus_tag="PZ377_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDM6357049.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(19268..19636)
                     /gene="rplL"
                     /locus_tag="PZ377_000157"
     CDS             complement(19268..19636)
                     /gene="rplL"
                     /locus_tag="PZ377_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDM6357050.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(19679..20179)
                     /locus_tag="PZ377_000158"
     CDS             complement(19679..20179)
                     /locus_tag="PZ377_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDM6357051.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     misc_feature    complement(20217..20363)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20451..21143)
                     /gene="rplA"
                     /locus_tag="PZ377_000159"
     CDS             complement(20451..21143)
                     /gene="rplA"
                     /locus_tag="PZ377_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDM6357052.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     gene            complement(21351..21773)
                     /gene="rplK"
                     /locus_tag="PZ377_000160"
     CDS             complement(21351..21773)
                     /gene="rplK"
                     /locus_tag="PZ377_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDM6357053.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            complement(21954..22502)
                     /gene="nusG"
                     /locus_tag="PZ377_000161"
     CDS             complement(21954..22502)
                     /gene="nusG"
                     /locus_tag="PZ377_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDM6357054.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            complement(22515..22697)
                     /gene="secE"
                     /locus_tag="PZ377_000162"
     CDS             complement(22515..22697)
                     /gene="secE"
                     /locus_tag="PZ377_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDM6357055.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            complement(22753..22896)
                     /gene="rpmG"
                     /locus_tag="PZ377_000163"
     CDS             complement(22753..22896)
                     /gene="rpmG"
                     /locus_tag="PZ377_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDM6357056.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            complement(23011..23580)
                     /locus_tag="PZ377_000164"
     CDS             complement(23011..23580)
                     /locus_tag="PZ377_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDM6357057.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            complement(23661..24185)
                     /locus_tag="PZ377_000165"
     CDS             complement(23661..24185)
                     /locus_tag="PZ377_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDM6357058.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            complement(24185..24931)
                     /gene="rlmB"
                     /locus_tag="PZ377_000166"
     CDS             complement(24185..24931)
                     /gene="rlmB"
                     /locus_tag="PZ377_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDM6357059.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            complement(24939..25343)
                     /locus_tag="PZ377_000167"
     CDS             complement(24939..25343)
                     /locus_tag="PZ377_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDM6357060.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            complement(25336..26736)
                     /locus_tag="PZ377_000168"
     CDS             complement(25336..26736)
                     /locus_tag="PZ377_000168"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDM6357061.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            complement(26720..27367)
                     /gene="cysE"
                     /locus_tag="PZ377_000169"
     CDS             complement(26720..27367)
                     /gene="cysE"
                     /locus_tag="PZ377_000169"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDM6357062.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            complement(27790..29244)
                     /locus_tag="PZ377_000170"
     CDS             complement(27790..29244)
                     /locus_tag="PZ377_000170"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDM6357063.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            complement(29801..30874)
                     /locus_tag="PZ377_000171"
     CDS             complement(29801..30874)
                     /locus_tag="PZ377_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDM6357064.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(30899..32263)
                     /gene="radA"
                     /locus_tag="PZ377_000172"
     CDS             complement(30899..32263)
                     /gene="radA"
                     /locus_tag="PZ377_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDM6357065.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            complement(32748..35204)
                     /locus_tag="PZ377_000173"
     CDS             complement(32748..35204)
                     /locus_tag="PZ377_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDM6357066.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(35218..36225)
                     /locus_tag="PZ377_000174"
     CDS             complement(35218..36225)
                     /locus_tag="PZ377_000174"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDM6357067.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(36215..36781)
                     /locus_tag="PZ377_000175"
     CDS             complement(36215..36781)
                     /locus_tag="PZ377_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDM6357068.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(36800..37261)
                     /locus_tag="PZ377_000176"
     CDS             complement(36800..37261)
                     /locus_tag="PZ377_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDM6357069.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            37419..38633
                     /locus_tag="PZ377_000177"
     CDS             37419..38633
                     /locus_tag="PZ377_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDM6357070.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(38841..39401)
                     /gene="pdxT"
                     /locus_tag="PZ377_000178"
     CDS             complement(38841..39401)
                     /gene="pdxT"
                     /locus_tag="PZ377_000178"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDM6357071.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(39405..40292)
                     /gene="pdxS"
                     /locus_tag="PZ377_000179"
     CDS             complement(39405..40292)
                     /gene="pdxS"
                     /locus_tag="PZ377_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDM6357072.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            40396..41778
                     /locus_tag="PZ377_000180"
     CDS             40396..41778
                     /locus_tag="PZ377_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDM6357073.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT        12579 a         8110 c         6137 g        14995 t
ORIGIN      
        1 aatattcatt ttcataataa ttataatata aaagcgcttt cttgtatata tgacatgtac
       61 atgttgctga tatttctgta aatggaaatt ctagttgtat taattgattt tagtaattta
      121 tagcgtttat tattgctaat tactgtcaaa ttaaattttt tatccctcaa ctcttaaact
      181 ctggatatct ttcattatat tagctttttt ataaccatgg atatcatgta aagccttata
      241 agctttaaat aatgtttcat acctttgtac tttttccgct tctggattaa tttcgtataa
      301 aattggctct ttcatagcat caactgcgtc attaattgaa ttatgtgcgc caccactgac
      361 tgcacctaat attgctgcgc ctattgctgg tgcatactca ctatccataa caattagttt
      421 tttattcagt acattcgcat agatatccat cataacagca ttctttttag gtataccgcc
      481 acatgcaaat accttttcta cttccatatg ccaatcttga tactgttgca taatcatctt
      541 agtaccaaat gctgtagctt ctaaatatgc tctataaata tcctcatgct tagtttgtaa
      601 agttaatcca aagatacaac ctgttaaatt gctatcactt aatacacttc gatttccatt
      661 atgccaatca agagcaatga gcccactttc acctggcatt tgatgtttta tcttttcatt
      721 cattaattca aatacagtca tatttctatt ttctgcttca tctacatatg actttggtgc
      781 ttgcttagcg acatactcaa acaaatcacc tactgctgat tgccccgctt cataagcaaa
      841 taattctgga ataattgctc cttttacaga acctgatata cctggcactt gatgttgctt
      901 ttcatttaac ataagatggc atgtgcttgt tcccatcacc atagtcattt ctttatcttt
      961 ttcagaccca atacctaata aactagcatg ggcatcaata ataaaaggac ttaccatagt
     1021 ttcttttgat aatcctaatt tctgtgccat tttatcatcc agtttcccta ctgcttcacc
     1081 aatattaaca accggtgcag atactttatc ttgaattact tttgataatt tggggtctat
     1141 tttatcaaat aaatcataat gaaaccctgt ttcttcttcc caaaatgctt tgaaacctaa
     1201 tccacaattc gagcgtacat ttttattagt taatttattt acaatccaat cgcccgcttc
     1261 cataatataa gccgtttttt ccataatttc tggtgctcga ttcatgacct ccattatttt
     1321 gggaatcatc cattcactac taacattata tccataatgg cctaaccact tattattatt
     1381 ttcaatagca gtttgatata atttctctgc ctctttatat gcaccatgat gtttccaaag
     1441 tttcacatac gcatgtggat tgtttttaaa ttgttttaag ttatgtaccg ggttaaggtt
     1501 ttcgtcagta aaaataatag tagatgaagt aaagtctata ccaataccta ctatattgtc
     1561 tggatctatt tttgattcac gtactatata tgatattcct tcttccataa tttctaaata
     1621 atcattacta ttttgaagtg catatgtatg tggtattttc aaaccattta attcactctc
     1681 aatgacacca tgtgtatatg gtttcacaaa ttttgatact acttgaccgt tagttgtatt
     1741 aattaaaaac acacggcctg aagcagttcc ataatcaatt ccaatgctat aagacatcat
     1801 gtttcctcca tatcgctagt tgcggttatt agttttgcaa gcgttttctt aaatcatact
     1861 cataataggt tctattttac aagtatcact taaattttgc caaaaaaaaa gagccctcat
     1921 acatttacat atgaggctcc tctaattaat atttaattat ttattttgta ttgcatttaa
     1981 catttcatta actgctaatt ttcctaactc atttgaagct aaaggactgt cacctgttaa
     2041 taatttacga tcttttaacg ttcttcctgt catatcgtcg ttaactactt ttaatccttg
     2101 tttagttaat aaatcagcaa ctaaccattt caagcgtcca ggtaaataac ctatttcaat
     2161 atttgcacct tcatctaatg agtcagggaa gacacaaaca gagtatcctt ctaatggaga
     2221 tttttctctg ttaagccctg ctgaaagtag tgctgctggt ccatgacata atgtaactat
     2281 aaagcggtca ttgtctaatg cccaatctaa tgtttgttga acgtcctcac tttcagaaat
     2341 accaacaact gcagcatgtc cgcctgggat aaagacagat aaatagtctg aatcaggtcc
     2401 taattcattt ttaatcacat ctgctaattt ttttggctgt tttaattttt ctttcaattt
     2461 attataagta cttataactg cctcgtcttc agttggcata gcccataatt ctaatttaac
     2521 tggataacca gataatgtcg caacatcaac gtcaaaacct gcttccatta aatgatgtaa
     2581 aggtaataac atttcaacag gatgattacc cgtagagaac atttttccat tttccaataa
     2641 tacatatcgc tcttcagcag caatcatcaa tactttccat ttaccatctt tataggcacc
     2701 tttgtgttca acaccatcaa aatctgtttt aggtgctgta tattgactaa gggaatatgg
     2761 tgatgggaaa aatgcgttat cttctgcttt atctggcgtt ggttgcttac ttaattcatt
     2821 tacatcttgt gacatagttt attcctactt tctctatata gttactacta gtatagacat
     2881 atactacaca ctataaacat gtattactat taaaatgctc attatcaaaa ataatcaaaa
     2941 tgaatttctt tgaaaaaaat acaactaaat tctacaagat gcttttaaaa ttcaaacttc
     3001 aattttgggg agcactaaac attgatatac gaacttactg ttaataaact aaacattaaa
     3061 aaaagcccac gacaatattt gccgtgggaa taaataaata ttaaatcaac ttcatttctt
     3121 ttcctacttt ttcataagcc gcaattgctt catctaacat gtcttttgta tgtgcagctg
     3181 taggcatatt tcttacacgt cctgtacctc ttggtactgt tgggaaaacg atagatttca
     3241 catagacacc ttcgtctttt aaacgcttac taaattcttg agttgttttt tcatcaccaa
     3301 taattactgg tgtaattgga gtttctgact cacctgtatc atatcctaat tttgacaatc
     3361 cattttttaa atattgtgca ttgttccata atttatcatg taattcagtt gaatccatta
     3421 actttttaac tgcttcagtt attgctttgg tatccccagg tgctaatgat gtagagaata
     3481 agaatggtcg tgattgtgct tttaaccaat ctattaactc ttttgtacct gctacataac
     3541 cgccaacgac accaattgct ttagaaagcg tacctatttg gaaatcgatt ttatcttgta
     3601 aaccaaaatg tttaaccgta ccagcgcctt tacccataac acctgaacca tgagcgtcgt
     3661 caacataagt taataaacca aattcttctg caatttctac aatttcaggt aatttagcca
     3721 catcaccatc catactaaaa acgccatcag tgatatacat cactttattg tattgacctg
     3781 attcaactgc ttcttttgct ttcgcacgta aatcatccat gtctgaatgg ttaactcgaa
     3841 taattttagc tttagataag cgacatccat caataattga tgcatgatta agctcatctg
     3901 ataaaatagc atcattttta ttcatgacag ctgaaatagc agccatatta caattaaatc
     3961 ctgattgata agctattgca gcttctgttc ctttaaattt tgctagtgtt tcttctaatt
     4021 cgtcgtgtaa atctaatgta ccattgattg tacgaacagc gcctgcacct acaccatgtg
     4081 tatcaatagc tgcttttgca gctgatttca aatcttcatt tgttgctagt cctaaataat
     4141 tatttgaaga taagttaatg tatgatttcc cattgatttt gatttctggt ccgtttgcac
     4201 cttcaattgt atctatttca ttatacaaac cattttcttt tagataattt atattttcct
     4261 ctaaaaactc atgtaatgat tgaaccacag tatataatcc cccttaagta ttgtttggta
     4321 atacctttat catacagtaa ttctacataa cttgaaagcc ttatcattca tagataaaat
     4381 atctcaaagc aatcatttag tacgataatt tcataaaatg ttatactata aacattattt
     4441 tagaaagtag tgataagggt gttagattgg ttccaactag caaataataa agaaaataaa
     4501 acaattcaac tcagacgtta tttacaccaa tatcctgagt tatcttttga agaatttcaa
     4561 acacatgatt atattgttaa ccaattaagc cagttatctt gtgatattga aactcctatc
     4621 ggccgcaatg gtatcaaagc cacatttaaa ggcttgggca caggacctac cattgcatta
     4681 agagctgact ttgatgcgtt acctgtcgag gaattaaacg atgtgcctta taagtctaaa
     4741 aatccaggat gtatgcatgc gtgtgagcat gatggtcata cagcaatttt acttactgta
     4801 gcagagattt tagatgaaca taaacattta ttagaaggta atgttgtatt aatattccag
     4861 tacggtgaag aaatcatgcc aggtggttct caagaaatga tagatgctgg atgtctggaa
     4921 aatgtagata gaatttacgg tacacattta tggagtggtt atcctactgg aactattcat
     4981 tcacgtgcgg gggcaatcat ggcctctcca gatgaattta gcgtcacatt taaaggtcgt
     5041 ggtggtcatg gtgccaaacc tcatgaaacc attgacccaa tagtaatcat ggcagagttc
     5101 attttaagtg cacaaaaaat aatttcacgt acgatcgatc cagttaaaca agctgtactt
     5161 tcatttggaa tgattcaagc aggcaccaca gatagcgtta ttcctgatca agcattttgt
     5221 aaaggtacag ttagaacttt cgattcagat atacagaatc atgtaatgga caaaatggat
     5281 aagcttctac aaggtttagc aatagcaaat gatattaact atgatttgaa ttatattaaa
     5341 gggtatttgc ctgtccataa taacgagaaa gcatatcaag ttattaaaga agcaacaaat
     5401 gacttacatg tacgctttaa tgaatcagat ttaatgatga ttggagaaga tttttcacat
     5461 tatttgaaag tacgaccagg tgcattcttt ctgactggtt gtggtaatga aagtaaaggt
     5521 attacagcgc cacaccataa tcccaaattt gatattgatg aaaaatcatt aaaatacgct
     5581 gtagctgtat ttttaaaaat catagaatta gagcaagtat ttaaaacaaa ttaagcattt
     5641 aaattaattg aaagatatga atctgagaca taaactaatg aaatgctccc ttcaaagtag
     5701 acattgaaaa aatgaaaact ttgaagggag cattttacaa tgtccaagac tcaacaattt
     5761 tatttaaaca gtattatcag ttataagttt tattagtcag tctttcatca attattgttc
     5821 ttctaaatcg aatagccatt acaaaattta cacattaaaa aaagacccct cgattgaggg
     5881 atcttctttt tatataaaat ctaagaaatt agaaattatt taatgatttc agtaacaacg
     5941 cctgatccta cagtacgtcc accttcacgg attgagaaac gagtaccgtc ttcaatcgcg
     6001 attggagcga ttaattctac tgtcatttca acgttatcac caggcattac catttcagta
     6061 ccttctggta agtgaacaac accagttacg tcagtagtac ggaaatagaa ttgtggacga
     6121 tagtttgaga agaatggagt gtgacgtcca ccttcgtctt ttgataatac gtatacttct
     6181 gctttgaatt cagtatgtgg tgtaattgaa ccaggagcag ctaatacttg accacgttgt
     6241 acgtcttcac gagcaacacc acgtaataat gcaccaatgt tgtcaccagc ttcagcgtag
     6301 tctaataatt tacggaacat ttcaacacct gtaacagttg ttttagatgt gtcatgtaaa
     6361 ccgatgattt caacttcttc accaactttg atttgaccac gttcaacacg gcctgtagca
     6421 acagtaccac gaccagtgat tgagaatacg tcctcaactg gcatcatgaa tggtttgtca
     6481 gaatcacgtt ctggagttgg aatgtaagta tctacagctt ccattaattc taagattttt
     6541 tcttcgtatt gagcatcgcc ttctaaagct tttaatgctg aaccagcgat tacaggtaca
     6601 tcgtcacctg ggaagtcata ttcgcttaat aagtcacgaa cttccatttc tactaattct
     6661 aataattctt catcgtcaac catgtcaact ttgtttaaga atactactaa tgctggtaca
     6721 ccaacgttac gtgataaaag aatgtgttca cgagtttgtg gcattggacc gtcagcagca
     6781 gatactacta agataccgcc gtccatttga gcagcaccag tgatcatgtt tttaacgtag
     6841 tcagcgtgtc ctgggcagtc aacgtgagcg tagtgacgtt tgtcagtttg gtactcaatg
     6901 tgagaagtat tgattgtgat accacgttct ttttcttctg gagcgttgtc aatcatgtca
     6961 tatgattgtg caactgagtc accatttttt gctaatacag tagcgattgc tgctgttaat
     7021 gttgttttac catggtcaac gtgaccgata gtaccgatat tggcatgttc tttagaacga
     7081 tcgaattttt cttttgccat tataaaatct ctcctattct aattaaaaat aatatttaaa
     7141 tttatctctc atgatagttt ctcaccatca tgagaaatta tttatagcga tgcaaaaaga
     7201 attttacaat tcttttataa tgcattcacc ccaaaaaatc aaactataaa gatgatagct
     7261 tacatttaag ctcaccttgt attttaacct aggctagcta gtcaaaacaa gttatattat
     7321 tcacctttat ttttcttgat aatatcttca gcgattgatt ttggaacttc agcatagtga
     7381 tcgaagtaca tagtgtaagt accgcgacct tgagtgtttg aacgtaatga tgttgcataa
     7441 ccgaacattt ctgaaagtgg tacataagca ttaacaactt gtgcattacc acgaggttcc
     7501 ataccatcaa cacgtccacg acgagatgtt acgtcaccca tgatatcacc catgtactct
     7561 tcaggcattt caatagttac tttcatcatt ggttctaaga ttacaggatc acatttttta
     7621 gcagcttctt taagtgctaa tgatgcagca attttgaagg ccatttcaga tgaatcgaca
     7681 tcatggtatg aaccatcata taatttagct ttaacatcaa ttaaaggata acctgctaaa
     7741 acaccatttt ccatagcatc tttaagacca gcttctactg atggaatgta ttcacgagga
     7801 actacaccac caacgatagc gttttcgaat tcgaaacctg cgcctgtttc gtttggtgtg
     7861 aattcaatgt gaacatcacc gtattgacca cgaccaccag attgacgaga gaatttacct
     7921 tgaacttgtg cagatgattt gaatgtttca cgatatgaaa ccattggagc acctacgtta
     7981 cattcaacgt tgaattcttt cttcatacgg tctactaaga tgtctaagtg aagctcaccc
     8041 ataccaccga tgataacttg tccagtttct tcgtcagtgt gtgcatggaa tgttgggtct
     8101 tcttcttgta atttaactaa agcttgagtc attttatctt ggtcagcttt agattttggc
     8161 tctactgata agtgaataac tggctctggg aattccattg attccaagat aatgtcattt
     8221 ttctcaccac ataaagtatc accagtacct gtatctttaa gacctaccgc agcagcgata
     8281 tctccagagt atacagtatc gatttcttga cgtgagttag cgtgcatttg taataaacga
     8341 cctacacgtt cacgtttacc tttagtagag ttcttaacgt atgaaccaga tgtcattgta
     8401 cctgaataca cacggaagaa tgttaattta ccaacataag ggtcagtcat aactttgaac
     8461 gctaatgcag cgaattcagc tgaatcgtct gctttcgcga ttacttcttc ttcagggttg
     8521 ctagcacggt gaccaataat tggtttaacg tctagtggtg aaggtaagta atcaattaca
     8581 gcgtcaagca ttaattgaac acctttgttt ttgaaagctg taccacaaag tactgggtag
     8641 aattctacgt tagtagtagc ttggcggata gcttctttta attcagaaac tgaaatttct
     8701 tcgtcaccaa gatatttttc cattaattcg tcgctagttt ctgcaactgc ttcgattaag
     8761 ctagcacgag cttcttcagc tctatctaag tggtcttcgg gaatttcaat ttcttcaatt
     8821 tcagtaccta aatcatttgt atatttgaaa catttcattt caactaagtc aatgattgct
     8881 tcgaattcgt cttccgcacc aattggtaat tggattggag cagcgttagc ttgtaaacga
     8941 tcatgtaatg tacttacaga gtattcgaag ttagcaccta atttgtccat tttgtttaca
     9001 aatacgatac gtggaacacc ataagttgta gcctgacgcc aaactgtttc agtttgaggt
     9061 tcaacacctg attgtgcatc aagtactgta actgctccgt caagtacacg taatgaacgt
     9121 tcaacttcta cagtgaagtc tacgtgtcca ggtgtatcga taatgtttac acggtgacct
     9181 tcccaagctg ctgttgttgc agcagatgtg atagtaatac cacggtcttg ttcttgctcc
     9241 atccagtcca tttgtgaagc accttcgtgt gtttcaccaa ttttgtggat acggccagtg
     9301 taataaagaa tacgttcagt cgtagtcgtt ttaccagcat caatgtgagc catgataccg
     9361 atattacgag ttttttctaa tgaaaattct ctagccatgt attttttctc cttccagttt
     9421 atattactga ataaatacga tagatatggc gatgccctaa gcatgaacgc ttaatgaaaa
     9481 ttcattaata tagaacacac tcagggtaaa agcttttatc ttaccaacgg tagtgagcaa
     9541 atgctttgtt tgcttcagcc attttgtgag tgtcctcacg tttcttaacg gcaccacctg
     9601 tattatttgc tgcatctaaa atttcgttag ctaaacgatc ttccatcgtt ttttcaccac
     9661 gaagacgtgc atagttaact aaccaacgta aacctaaagt agtacgacgc tctggacgaa
     9721 cttctactgg tacttgatag ttagaaccac ctacgcgacg agctttaact tctaatactg
     9781 gcataatgtt gttgattgct tcttcgaata cttctaatgc atcacgacca ctgcgttgtt
     9841 caactaggtc gaatgctgaa taaagaattc tttgtgctgt tccacgttta ccatctaaca
     9901 taattttgtt aattaattta gttactaact tagagttatg aattggatct ggtaatacgt
     9961 ctcttttagg tactgatcct ttacgaggca taatgtaaat cctcccttcc ttatattaaa
    10021 tattttaagt ttaagattta attaaaaact aaattcttag tttttaggtt tcttagttcc
    10081 gtataatgaa cgaccttgtc tacgtccgtc aacacctgaa gtatcaagtg ctccacgtac
    10141 aatatggtaa cgcacacctg gtaagtcttt tacacgtcca ccacgtacaa gtacaacact
    10201 gtgttcttgt aagttatgtc cgataccagg gatgtatgcg ttaatttcga tgttgtttga
    10261 taaacgcaca cgtgcatatt tacgtaacgc tgagttaggt tttttaggtg tcattgtacc
    10321 tacacgagta catacaccac gtttttgtgg tgagtttaag tcagtaaatt ttttcttttt
    10381 actgttgaaa cctttattta aagctggaga atctgatttt ttgattttgc tttgtcttgg
    10441 tttacgtact aattggttaa tagttggcat gtgatatgtc ctcctctctt cactttttaa
    10501 tcccacacat ccaggtggtt catttttagg ttaaataaaa tttagtaagt aaaacactta
    10561 ctaattctca tttaatcaat gctactattg tcgcattgac gttaatacct acatgtttac
    10621 ccaaagcatg tttgcttttg aaaaaagata caggtatatt tttctgattg atttggctta
    10681 acacgcgagt cattaaatat acttcaacgt cttcagcaat aatcaaagat gtaacttgat
    10741 ctttctttaa cgctttaagc gtttctttaa gaccaactac aaaatgttgt ttgttaaagc
    10801 gtgcaacttt ttccttagac aataatttat cctccaaagt tctgcttgca tcaaccttta
    10861 atatattaac attgaacaag agaattcgtc aacattaaat caaataattt acataaaaat
    10921 aacaagaggc taaagcatta gcctctgtta tatacttgtt attccgttac ttcagtttga
    10981 gattcaactt ctgcatctgg tttagctgtt ttttcgtatt ttacgtcgct ataacgtctc
    11041 ataccagtac cagctggaat taacttacca ataattacgt tttctttaag acctaataag
    11101 tcatcacgct tacctttaat tgctgcatct gtaagaactc ttgttgtttc ttggaatgat
    11161 gctgcagata agaaactttc tgtttcaagt gatgctttag taataccaag taatactggt
    11221 ttagctgttg caggacgctt acggtgttta aatgcttctc tatttgcatc tgtaaagtta
    11281 tgaatatcaa ctaatgaacc tggtaataac ttcgtatcac ctgcttcgat aattctaact
    11341 ttacgtaaca tttgtcgaac cataacctca acgtgtttat cgtcgatttc tacaccttgc
    11401 atacggtaaa ctttttgtac ttcttttaat aagtagcttt cagtcgcgtt taatccagca
    11461 acagataagt aattcttagg ttcaatagaa ccttcagtta atacttcacc acgttgaact
    11521 ggttgaccga tttctacaat aattcttgaa gtacctgaag caaggtatga tcttgtttca
    11581 ttagcacctt taacaacaat ttcttgttgt ctatcttttg ctaatttaat atcttctacg
    11641 acaccttcga tttccgtaat taccgcttga cctttagggt tacgtgcttc gaaaatctct
    11701 tgaatacgag gaagaccttg tgtgatatcg ctacctgcta ccccacctgt atggaatgta
    11761 cgcattgtaa gctgtgtacc tggttcaccg atagattggg ctgcaattgt accaactgct
    11821 tcaccaactt caactttttc accagtagca aggtttttac cgtaacattt ttcacaaaca
    11881 ccatgtcgtg cgttacaagt aaatgctgag cgaatataca tttgttcaat accagcatct
    11941 gtaattttct tagcaatttc aggtgtaatt aattcatcag gacgaatgat tatttcatca
    12001 gtttcaggat gatgaattgt ttctttagaa taacgacctt caatacgttc gataaatggt
    12061 tcaatcattt ctgtaccttc tttaatatca gaaactaata aacctctatc agtaccacag
    12121 tcttcttcac gaacaataac atcttgtgcc acgtcaacaa gacgacgagt aagatatcct
    12181 gagtcagctg ttttaagtgc tgtatcggca agacctttac gtgcaccgtg agttgagatg
    12241 aagtattcaa gtactgttaa accttcacgg aatgaagatg tgattggtaa ttcgataatc
    12301 ttaccagatg gtgcggccat caatccacgc atacctgcta actgtgtaaa gttagatgcg
    12361 ttaccacggg cacctgaatc actcatcatg aagattgggt tagttttatc aagtgattgc
    12421 atcaattcac cttgaatttg atcttttgca tctgtccaaa tttcaacaac tgcattatat
    12481 ctttcttctt cagtgattaa accacggttg aattgttttg taattctgtc gactaatttt
    12541 tcatgctcat caagtatttg ttgcttatca ggtaatacta cgatatcagc aacacctact
    12601 gtaataccag ctttagatga gaatttgaat cctaagtctt tcatacggtc taacatcatt
    12661 gatgtatcag tgatgctaaa tctgttgaat acttctgcaa taatattacc taagaatttt
    12721 ttgttgaaag gttcaattaa ttcttcattt tcaaagtatt cttttaatcc accttcacct
    12781 aaagttgtag gatcgatgaa atatctgttt ggtgtctttc tttctaagtt ttcttgcgta
    12841 ggttcattaa tataagcaaa tgaatctgga atgatttcat tgaatataat tttacctact
    12901 gacgtagcaa gaatcttttt gttttgttct tcagtaaatg ttggattatt gaacgaactt
    12961 gcatgtacac caattctagt gtgtaaatgt acaaagccat ttgcatatgc ttttaatact
    13021 tcatttgtat tattaaagat tgcgcctgta tttactgcat cttttctttc taaagtaagg
    13081 taatagttac caagtaccat atcttgtgat ggtgtaacta caggtttacc atctttaggg
    13141 ttcaagatgt tttgtgctgc taacatcaac attcttgctt cagcttgtgc ctcttttgat
    13201 aaaggaacgt gaaccgccat ttggtcaccg tcaaagtcag cgttataagc tgttgttaca
    13261 agtggatgta gacgaatcgc acgaccttca actaaagttg gttcaaatgc ttgaatacca
    13321 agtctatgaa gtgttggtgc acggttaagt aatacaggat gttctctaat tacttcttcc
    13381 aatacgtccc aaacttcatc atccatacgt tcgattttac tcttcgcatt tttaatgtta
    13441 gttgcaattt cacgttgaac taattctttc attacgaatg gtttaaatag ttcaagtgcc
    13501 atttcttttg gtaaaccaca ttggtacatt ttcaagcttg gacctactgc aataactgaa
    13561 cgtcctgaat agtcaacacg tttaccaagt aagttttgac ggaaacgacc ttgtttacct
    13621 tttaacatat gagataaaga ttttaatgga cggttacctg ggccagtaac tggacgacca
    13681 cgacgaccat tatcaattaa agcgtcaacg gcttcttgta acatacgttt ttcgttttga
    13741 acgatgatac caggtgcacc taaatctaat aaacgtttca aacgattatt tcgattaatt
    13801 acacgacggt ataagtcgtt taagtcactt gttgcaaatc gtccaccatc taattgaacc
    13861 attggacgaa tttctggtgg gatgattgga agtacatcta aaatcatcca tgaaggtttg
    13921 ttacctgaat tacggaatga ttcaacaact tctaaacgtt taattgcacg agtaagtctt
    13981 tgaccagtag ctgattccaa ctcatcgcgt aacaatttaa gttcttcgtc aagatcaatc
    14041 tcttcaagta aatctttaat accttctgca cccatttttg caacgaattg acctgggtat
    14101 ttatcataat aatctctgaa ttcagcttca gataataaag ttttcttttc taaaccagtt
    14161 ggacctggat ctacaacaac ataagaagca aagtaaataa cttcttctaa tgctcttggt
    14221 gacatgtcaa gtaataatcc catacgactt ggtatacctt tgaaatacca aatgtgagaa
    14281 actggagcag caagttcaat gtgacccatt ctttcacgac gtactttaga tttagttact
    14341 tcaactccac atctgtcaca gaccatgcct ttgtagcgaa cacgtttgta tttaccacaa
    14401 ctacattccc agtcttttgt aggtccgaaa attctttcac agaatagacc atctttttca
    14461 ggttttaatg tacggtagtt gattgtttca ggttttttaa cttcaccaaa agaccaagaa
    14521 cggatttttt caggtgaagc caatcctatt ttcatataat ggaaattatt tacatcaatc
    14581 aaggagccta cctccttcaa tttagattag ctgtgctatt tgattgattt gcatttattt
    14641 aaaatggctt aaagtactaa actgaatcat caataaaatt cagcttagta tctttttgcc
    14701 tgttttgtaa attgcgtatt aatcagtaac ttctttttgt gtttcaggag catcattttg
    14761 ttgtaaatct actttgcgtt ctacaacatc atcgtcatca acgtctgtca tttcgatttc
    14821 attatcttgc tcatccataa cttttacatc taaacctaaa ctttgtaatt ctttcatcaa
    14881 tactcggaat gattctggaa cacttggtct agagatgttt tcacctttaa caatagcctc
    14941 gtatgttttc acacgtccta ctgtatcatc ggatttgtaa gttaagattt cttgtaatgt
    15001 gtatgcagca ccatatgctt caagtgccca tacctccatc tcaccaaaac gttgtccacc
    15061 gaattgcgct ttaccgccaa gtggttgttg tgtaacaagt gaatatggtc ctgttgaacg
    15121 cgcatgtaat ttatcatcaa ccatgtgcgc aagtttcaac atgtacatta cacctactga
    15181 aatacggtta tcgaatggtt cacctgtacg tccatcataa agtacagttt taccatcacg
    15241 agccatacca gcttcttcaa ttgttgacca tacatcgtca tcgtttgcac cgtcaaatac
    15301 tggtgatgca acgtgaatac caagattttt agcagccata cctaagtgta gctctaatac
    15361 ttgtccgatg ttcatacgag atggtacacc aagaggattt aacatgatat cgatcggacg
    15421 tccatctggt aagtaaggca tatcttcttc aggaacaatc ttagaaatga cacctttgtt
    15481 accatgtcga ccacacatct tatcaccaac atgaatttta cgtttttgaa cgatatatac
    15541 acgtactaat tggtttacac caggtgataa tgtatcgtcg ccttcttcac gattgaatac
    15601 ttttacatca agaacgatac cgccagcgcc gtgaggtaca cgtaatgaag tatctctaac
    15661 ttcacgtgct ttttcaccaa agattgcatg taacaatctt tcttcggcag ttaactcagt
    15721 tacaccttta ggcgttactt taccaactaa aatatctcca tcttttactt ctgcaccaat
    15781 ataaacgata ccacgatcgt ctaagttctt aagtgcactt tcagaaacat taggaatatc
    15841 tcttgtgatt tcttcaggtc ctaacttagt atcacgtgct tctgattcat actcttcaat
    15901 atgaatagaa gtatacacgt catctttcac aagtctttca ctcatgataa cggcatcctc
    15961 atagttgtaa ccgtcccaag tcatgaaacc aactactacg tttctaccta atgccatttc
    16021 tcctaattcc atagatggtc catctgctaa aatctcgtta tactcaacaa catctccaac
    16081 tgcaacgatt ggacgttggt tgtaacatgt acctgagttt gaacgtttaa atttagctaa
    16141 tggatagcga tctaattcac cttcatgctc aacgccgttc tcttcaacta gacgacgaac
    16201 aagaatttca ttagattcaa catgttcaac acgacctctg tgcttagctg taatagccgc
    16261 accagaatca cgtgctgcaa cgtgttccat acctgtacca acaaatggtg cttctggatt
    16321 catcaaaggc actgcttgac gttgcatgtt cgcacccatc aatgcacggt ttgagtcatc
    16381 attttctaag aatggaatac atgctgtcgc tgctgaaaca acttgcttcg gcgatacatc
    16441 catataatcc attttttctt tagccataac tgtattgtta ccacggaaac gacatacaac
    16501 ttcatcatcc atgaaacgac cattttcatc taatttagag tttgcttgtg ctacaacata
    16561 gctatcttct tcgtcagctg ttaaatagtc aatttgatca gtgatagcat gtgtatctaa
    16621 atcaacttta cgatatggtg tttcaataaa gccgaattca tttacacgtg cataacttga
    16681 taatgagtta atcaatccaa tgtttggtcc ctcaggtgtt tcaattggac acatacggcc
    16741 atagtgagag tagtgaacgt cacgtacttc catttgagca cgttcacgtg ttaaaccacc
    16801 aggtcctaat gctgatagac gacgtttatg cgttaactca gctaatgggt ttgcttggtc
    16861 catgaattgt gataattgag agctaccaaa gaattcttta atagatgcaa taacaggtcg
    16921 aatattaatt aattgttgag gtgtgataga ctcagtatct tgaattgaca ttctttcacg
    16981 tacaactctt tccattcttg ataaaccgat acggaattgg ttttgtagta attcacctac
    17041 agaacgtaaa cgacggttac ctaaatggtc aatatcatct gtatatccaa taccgcttaa
    17101 taagttaaag aagtaactca ttgaagcaat gatatctgct ggtgtaatgc atttaacttc
    17161 tgagtcaggg aaagcattac caattacagt tgtcgtacga ccttcatcat cgttaggaac
    17221 atatacttta attgattgaa tttctactgg ctcgtctata acgctaccat gcaattcaaa
    17281 cacttcgctg tttgcatttg attcaagtac atccatgatt tcgtcgattt tacgacgatc
    17341 aagcactgta ccttcttcaa ctacaatttc accagtttca gtatttacaa ttggctcagc
    17401 taatttttga ttaaataaac gatgttttaa atgtaatttt ttgtttgttt tataacgacc
    17461 cacgcttgct aagtcatagc gttttggatc aaagaaacgt gaatacaata gacttttagc
    17521 attttcaaca gttggtggtt cacctggacg taaacgttca tagatttcta ataacgcttg
    17581 ttcagtgttt tcagtgccgt ctttctctaa agtattacgt aaatattcat tgtcacctaa
    17641 aaggtcaaca atttcttggt cgcttgagaa acctaatgca cgtaacaata ctgttaatgg
    17701 tagtttacgt gttctatcaa tacgtacgta tacaacatct ttagcatctg tttcatattc
    17761 taaccatgca ccacggtttg gaataattgt tgcatcatag ttttcacgac catttttgtc
    17821 gattttttca ttgaaataaa cggatggtga acgaactaat tgagatacga ttacacgttc
    17881 tgcaccattg ataacgaacg tacctgtatc agtcattaat gggaaatcac ccataaagac
    17941 ttcttgttct ttaacttctc ctgtttcttt aatgattaga cgcactttta cacgaagagg
    18001 tgcagcataa gtagcgtcac ggtttttaga ttcttctaaa tcatattttg gttctcctaa
    18061 acggtaatcc acaaactcta atgacaaatt accagtaaaa tcttcaattg gagaaatgtc
    18121 tctaaacatt tcgattaaac cttctcttag gaaccactcg taagatttag tttgaatttc
    18181 tattaagttt ggtaattcta atacttctga aattctcgcg tagtttctac gtttacgatg
    18241 tcttccatat tggacaactt gacctgccaa acagattcac ccctcaaaaa ttatgtgatc
    18301 cgcttttagc taaataccat aagcatatta ataaaaagac aaaaagaaaa cggcaacact
    18361 aatgatgaca ccattttcga ttctatattt attcttaatt aacttatcta tttaacgtaa
    18421 aagtacacac ttattgaaca taaattttta cattatataa ctataacaga atatccattt
    18481 catttcaagc ttttatactt ctcagaatgt aatatccttt atctttattt accacttcta
    18541 catttccaaa aagttcattc attcttttct ttgcagatgg cataccttgc ttcttctgaa
    18601 ttacaacgaa tagttcaccg ttcgagtcta atctatgtaa tgcttgctcg aatatacgat
    18661 gcacggtttc tttccctgct cttattggtg gattggttaa aataaaatca aaacttttgt
    18721 cttccacagc agacaaagca tcactttcct ttacgatcac attatcaata ccatttaatt
    18781 ttttgttttt ttcaactaag gctagcgctc tgtgattaac atctagcatt gtaattgaat
    18841 gatgtggtga tactttagca atcatcaaac caattggtcc gtaaccacaa ccaacatcgg
    18901 caattcgctt acttggacca ggtggatgcg cttttaaaaa agtttgaaca agaacatctg
    18961 aaccataatc tactttatct ttcgaaaaca ctccgttatc agttattaaa tcaattttat
    19021 gatggtttaa ttgatattga atacgttgtt cattgctaat tacacttgga tcttcatcgt
    19081 aataatgact cattattcca cctcatcttc actttgtaac tcgtcattat aattataaca
    19141 tttcactcag tctttaaatg aataaaaccc cgttatcaaa ataacggggt tttaacttct
    19201 caaagaatca ttatattata gcaatacttg ctataaaact ttgattatta agtttaagat
    19261 acttgaatta ttttaattct acagtagctc caacttcttc taattgttct ttaagttttt
    19321 cagcttcttc tttaggtaaa gcttctttga ttactttagg agctccgtct actaattctt
    19381 tagcatcttt taatcctaaa ccagttgctt ctttaacagc tttaacaact ttgattttag
    19441 atgaaccagc tgaagttaac tcaacgtcaa attcagtttt ttctgctgca gcgtcagcgc
    19501 caccagctgc acctgctact gctactggag cagctgcagt tacaccaaat tcttcttcaa
    19561 ttgcttttac taagtcgttt aattctaata ctgacatttc tttaatcgct tcaatgattt
    19621 gttcatgatt agccatttta taattcctcc attattttta atttttttac gctaaaaatt
    19681 attcagcgtt ttcttctttt tgttctccaa tagctttaac cgcataagcg aagttgcgta
    19741 caggagcttg taatactgat aaaagcatag atacaagacc atcgtgtgaa ggtaatgaac
    19801 caacagtttt aacttcttct gctgtaataa cattgccttc cataacgcct gatttaattt
    19861 ctaatgcttc atgatcttta gcaaatccag aaattacttt cgctgcagct acagcatctt
    19921 cacttgaagt tgcaatagca gtaggacctg ttaagaattc atctaagcct tcgataccag
    19981 ctttttcagc tgcacgacgt accatagtgt ttttgtatac tttatactca acaccagctt
    20041 cacgtaattg tgaacgtaag tcagtaactt cagctactgt taatccacgg tagtcaacga
    20101 ttactgttga aactgaattt gatagtacct cagcaatttc atcaactagt tgttttttag
    20161 cttcaatgat agcagacatt cagacacctc catttaaatt tttggtgctt tttttaaaat
    20221 taataaaaaa agcactttct acccacggca aaaagtgctt gaaagatcat agatcatcac
    20281 gttcaagtca attttagcct cggcaggata aattttaagt tataaataac tcctactgtc
    20341 ttaggtaaaa taatcacaat aagtaatata acttggaaat tacttattgt caatatattt
    20401 ttacagactg aaagatattt ctttcagcct gtaattgttt atatcattta ttatttgaaa
    20461 cttgcagtat caattttaac tccaggaccc attgttgtag ttacagcaac agatttgaag
    20521 tatgtacctt tagcagatga tggtttagct ttagctaata catcttgtaa agtattgaag
    20581 ttttcaatta attgttcatc agtaaatgaa actttaccaa ttgatgcatg tacgatacca
    20641 gctttttcag cacgatattc tactttacca gctttgattt cttcaacagc ttttttaaca
    20701 tccattgtta cagttccagt tttagggttt ggcattaaac cttttggtcc taatacacga
    20761 cctaatttac caacttcacc catcatgtct ggtgtagcaa ctactacatc gaagtcgaac
    20821 caaccttgtt ggattttttg aacgtattct gcttcaccta catagtcagc acctgctgct
    20881 tcagcttcag caattttgtc acctttagcg aatactaata cactttgtga tttaccagtt
    20941 ccgtttggta atacaactgc accacggatt tgttggtcat ttttacgtgt atcaattcct
    21001 aaacggaatg caacttcaac agaagcgtca aagttagcaa tgcttgtttc tttagctaat
    21061 ttaattgctt cttcaacact gtagtgctga gtacggtcaa ctttactagc tgcttcttga
    21121 tactttttac cttttttagc catttatagt tcctccttta gtggttttag cggaatttcc
    21181 tcccacatta cttgctatat gcaagttaag agcagacaac agaagaatta atcttatgaa
    21241 taaatcaatc attaatctag tcattcagat tatatttata attcatcatt attatctctg
    21301 ttatctgctt catcgtttaa atcatgtaat caaatttaat attcgtaaaa ttattctaca
    21361 acgataccca tactacgtgc agtaccttcg ataatacgca tagctgcttc ttcgtcagca
    21421 gcgtttaagt cttgcatttt gctgttagca atttcgcgta cttgatcttt agttactgta
    21481 gcaactttag ttttgtttgg ttcgcctgaa cctttttcaa taccagctgc ttttttaagt
    21541 aatactggag ccggtggagt ttttgtaata aatgtaaatg aacgatcttc ataaacactg
    21601 atttctaccg gaataattaa acctgcttga tcttgagtac gtgcattgaa ctctttacag
    21661 aatcccatga tgttcacacc tgcttgacct aatgctggac caactggtgg tgctggattc
    21721 gctttacctg caggaatttg taatttaaca actttatcta cttttttagc cacgatgtgc
    21781 acctccttga tatcgtgatg tggtcacagg gctcagtttt gccctcccac tcttaacatt
    21841 tcgtgacgaa atgggcgctc taaaagcgcg accacataat tttaacactt ttctaattaa
    21901 aaaatcaata ctctttttta tcttttggtt agtttaataa ctttaattgt taattaaagc
    21961 ttttctattt gatcgaattc aacttctact ggtgtttctc ggccaaacat atctactaat
    22021 actgttagct taaacttatc tgtttcaatt tcttgaactt caccaacttg attcgcaaat
    22081 ggacctgatt taatacgaac ttgctcgcca acttcgagtt caacatcgat agtcttttct
    22141 ttaagaccca tttgttttaa gatgaagcga acttcttctg gtaacaatgg atttggctta
    22201 gaccctgcac ctgcagaacc tacaaaacca gtaacgcctg gtgtatttct taccacatac
    22261 catgattcat ctgtcatgat taattccact aaaacatatc cagggaatgt ttttttaaca
    22321 gtcgttttag ctttgccatc ttttacttga gtttcttctt cttccggtat gactactcta
    22381 aagatttgtt cagtcatatt catagattct actctttttt ctaaattctt tttaacttta
    22441 ttttcatatc cagaatatgt atgcactgca taccaacgct ttgcgccaac ttcttcagac
    22501 atgtcttcac tcctctaacg aaataataaa tttttcaatg ctgtaattcc taaatctaag
    22561 gcatagaaaa agactaagaa gaatataaca gtagaaacta caattacagt atatttaaat
    22621 agctcttctt tcgtcggcca acttgttttt tccatttcag acttaacgcc tttaaagaaa
    22681 ctttcttttt tagccatctg ctcaacctcc aaatcctttg aacatattat caaagtatta
    22741 tttcgaatgt atttatttcg attctttatg aattgtgtgc gcgttacatt ttggacaata
    22801 tttctttaag gttaatcttg ttgccgagcc ttcttgctta ggaacattat aatttctatt
    22861 gccacaagct tcacaattta aaggtatttt tctcacaata attcaccttt attcattata
    22921 ataccagtat actatacata aaatttataa caaatgtcaa accatcagta agtaacacgt
    22981 tataattcaa ttcacgtcct aaggcgcttt tcaaatcatt ttgaaataac gttttatttt
    23041 atttttacat cgttgtatgg cattataaat cactttctct tttacatgca tcaattgagc
    23101 aatttctctc ggcttatatt gttcacacat taaatacatg acatcttttt caaatgcact
    23161 caaatactta aactgacaaa tcaactcttt aattgctaat ttattcaaat aattgttttc
    23221 aacatcaaaa cgctttattg cacatggata cgtaactcta tattcattaa tcaaattatc
    23281 cattctttta tttgtagcta aatattttct caaataatca tactttacag acgtaattaa
    23341 acattgaaca taatttgtga aaggctgctc tccactaaaa tcaaatgttt ggaccgacct
    23401 atacatccgt ataagtattt cttgatataa gtcttctaaa tcataatgat taaatccaaa
    23461 tgttttgatg cgtcgaataa ttaatggttg tagatccatt actaactttt cgaagtcttt
    23521 atcatttatt gcattgttac gtttgattgt actgtcttga gttgtcaaat cgtatttcaa
    23581 atattaaact aaccccttct atctaaaatt taaggttagt ttaatattgt tacattcaaa
    23641 atttcaagat gacggaaatg tcatttctta tgatgtcctc ttcgtatttt ttcaaattct
    23701 gcaaggattt cagaagataa cggaattcga gttcttggct tgttttcact tatatcatct
    23761 aatgatttac tcacatcaat ttcattttct tttaaatctc tccacatttc gcgagatgat
    23821 attctatatg cacctgatcc aaagatagca tgttgctcac tcatatcact tgttacaact
    23881 gtaatatgct tagtatgctt gtcataaagt tcataaacat aacgttctat gaaactatca
    23941 gctgtttctt tttccttggt aaaaatcgtt ttaacgccat gatacatgta ttctctttca
    24001 acacccgatt ggtcataagc atcgaaaaca caaataattt catctgaaat aactgcatta
    24061 taatttgcaa ttgcatctat taattgcatt ctagcttctt ctaaattctc ttttgcaatg
    24121 gcgcttagcg ttggtgattg tcctatcata ttgtatccat caatgattaa gtaacgttct
    24181 ttcattatat ttctccaaca tcatgtcttt ttcgaaatac ttcgtacatc attaaacttg
    24241 ctgcaaccga agcattcaaa ctgtttacat gtccaaccat tggaatctta atataaaaat
    24301 cgcatttatc acttactagg cgactcatac cctgtccttc gctaccaatt acaatagcca
    24361 atgacatgtc cgcttctaga tttctataat ctgttgcatt attagcttca gtgccagcta
    24421 cccaaaagcc attatctttt agttcatcga tagttttagc taaatttgtc actcgaataa
    24481 ctggtacatg ttcaattgca cctgttgagg cttttgcaac tgtttgcgtt agtgtaactg
    24541 aacgacgttt aggaataata acaccatcaa ctcccgttgc atcggctgtt cttaaaattg
    24601 atcccaagtt atgtgggtct tctaagccgt ctaatataag tactgtcaat aaaccttctt
    24661 tttctttttg ctgttttaaa aattgatcga agtcagcata ttcatatggt gcaataagcg
    24721 ctgcaacacc ctgatgtggt gcatttgcta aaaaatctaa tttagatttt ggtacagttt
    24781 gaacaatgat tttttgatct tttgcatttt ttaaaatttc attaatttgt tgctttttaa
    24841 taccttcttg aatcaatatc ttatttatcg gatgcccagt aataatcgct tctctaacag
    24901 catgcctacc aacaataacc gtatcttcca catcgtcact acctttcgtt tactattgtt
    24961 attattttat ttaataatgc ctctaatcgt tcttctcttt tttctaaata aagaaaacct
    25021 atcactgctt ctatcgctga acttttacga tatgtttgaa catcagtgtt tttagcttta
    25081 gtatgacttt tcgcgttacg ccctcgcttc aaaatatcca tttcttcgtc tgtaaaccat
    25141 tcttgctcca ttaaatattc taacgtttgc gcctgacttt tggcagatac atatttttta
    25201 gacatttgat gtagtttatt aggcttactt ttaagcttta aaacgatata ggtacgtaca
    25261 tattgatcta agactgcgtc tcccatatat gctaaggtca atggattcaa taatttaatg
    25321 tgattatctt gttgattatc cacgtttaaa tctaacccct tgaggtgtgt cttctaatat
    25381 aatgttttgt gatttcagca tgtctcgaat ttcatctgct cgtgcaaaat ctttgttttt
    25441 ccttgcttca ttacgctctt cgattaattt ttcaacatct tcatccaata attcatctgc
    25501 atttttagat tttaacggta cacctaaaac atcgctgaaa atttgataaa ctgctttaaa
    25561 tttatcaatt acttctgttg atgttgtgtt ctctagtaca tatttattcg caagttttgc
    25621 taaatcatac caagctgtaa ttgcattagc tgtattaaaa tcatcattca taactgtttc
    25681 aaaacgattt aaaatcgcat caatttgatc aatatatgtc tgttgatttt caatattagt
    25741 agcaatttgt gcgcgctctt caattaattg ataactattg cgaatacgct ctagtccact
    25801 acgtgctgat tctaccaatt ctagattata gttaattggg cttctataat gtacgctaat
    25861 cataaagaat cttagtacat ctggatcaac ttctttaata atatcgtgaa ctaaaataaa
    25921 gttgcctagt gatttactca ttttttcatt atcaatatta atgaaaccat tatgcatcca
    25981 ataattagca aatggcgcat gattatgtgc ttctgattgt gctatttcat tttcatgatg
    26041 tggaaattgt aaatctgaac cacccgcatg tatatcaatt gtaggtccta gctcatgaaa
    26101 tgccattaca gaacattcta tatgccatcc tggtctacct tcaccaaatg ggctatccca
    26161 actaatctcg ccaggcttcg cttttttcca caatgtaaaa tcaagtgcat cttctttatg
    26221 ctctcctgca tctatacgag cacccacttt taagtcatct atggattgat gacttaattt
    26281 accataacct tcaaatttac gtgttctaaa gtaaacatcg ccaccacttt catatgcata
    26341 accttgatcc accaaatctt taataaattg aataatgtca tccatatggt ccattaccct
    26401 tggatttgaa gtcgcttttc taacatttaa cgcaccaaca tcttcatgaa aagcagcgat
    26461 atatttttct gcaatttcgg gaacagactg atttaattct tgagaacgtt taattaattt
    26521 atcatctacg tctgtaaaat ttgatacata ttctacatta tatccttggt attcaaagta
    26581 acgtctcact acgtcataat taattgctgg tcttgcgtta ccaatatgaa tgtagttata
    26641 tacagtagga ccacatacat acatttttac tttccctggt tctataggct tgaacacttc
    26701 tttttgacgt gtaagcgtat tatataatgt aatcatcttg aatctctcca ttcctagtct
    26761 tttcaagttg tcgttctaaa tgcttaattt gttcataaat tggatcaggt agatggcgat
    26821 gatcaaatgt ttttccaact cgaacaccat cttgcttaac aatatgtcct ggtataccaa
    26881 caaccgttga ataacttgga actgattgta aaacaactga atttgcacca atatttacat
    26941 ttgaatttat tttaatattt cctaaaactt tcgcaccggc tgctattaaa acattgtctc
    27001 ctatatctgg gtgtcttttc cctctttctt tccctgtccc accaagtgtc acgccttgat
    27061 agattgtcac attatcacca attgtacatg tttctcctat tacaacgccc ataccatgat
    27121 ctataaatag acgctttcca attttagcac ctggatggat ttctatacct gtgaaaaatc
    27181 ttgaaatttg agatatcgcg cgtgctgcaa catatttttt ttggttgtat aacttatgtg
    27241 caatcaaatg actccaaact gcatgtaaac ctgcatacgt tgtaatgact tctaatgttg
    27301 aacgtgccgc tggatcctgc tcaaatacca tttttatatc gtctctcatt ctttttaaca
    27361 agatcatttc ctcctcaatg attgaactac gtaaatacat aattgaagta cctgcgaaat
    27421 taaatatcaa aaaagcacca ctaacataca agttgtattg ttagaggcgc ttccgcacgg
    27481 ttccactctg aatttagcga ataacattaa taatattgcg ggcgcttcca aattatcaag
    27541 gaaactaagt caacttaatg ctcatcactc tcattatata tttaattcat tttacgaagg
    27601 tgcattcatt aatttctacg ttgtactcac agcaaccgta cactctctgc atcgtataaa
    27661 tttaattact aatccttcgt tttatataaa atactttatt catattgttg taatttgtta
    27721 atacaacttt atcatatgtg gttacgttat aaaattcaag tatatactac cttgatcttg
    27781 tctatttcat tacttatatt gttttaaacg gtttagcact ttttctttac caagtacttc
    27841 aattgtattt ggtaattcag gaccatgcat ttggcctgtt acagcaacac gaataggcat
    27901 aaataattgc ttgcctttta ttcctgtttc tttttgaact tctttaattg tctttttaat
    27961 ttcagccgct tcaaatggtt caagtgcttc taatttactg aataagtgcg tcattaactc
    28021 tggtacttgc tctccattaa tcacttgttg ttcttcttca ccaagagctg gcatttcttt
    28081 aaagaacatt tctgataaag gtacaatttc accggcataa ctcatttctt tttgataaag
    28141 cgcaattaat ttgcgtcccc aagataaatc ctcttctgac ggcacctcag gaatcaaatt
    28201 tgctttaatt aaatgaggta atgctaattg gaatactgtt tcagtatctt tttgtttcat
    28261 atattggtta ttaacccatg ctaatttttg cttatcgaaa aatgctggtg attttgacaa
    28321 acgcttttca tcaaagattt tgataaattc ttctttagaa aagatttctt cttcaccttc
    28381 aggagaccaa cctaataacg caataaaatt aaataacgct tcaggtaaat aacctaagtc
    28441 acgatattgc tcaataaatt gtaaaatttg cccatcacgt ttacttaact ttttacgttc
    28501 ttcattaaca attaatgaca tatgaccaaa acgaggtggc tcccagccaa atgcttcata
    28561 aatcataatt tgtttaggcg tgtttgaaat atgatcatca ccacgaatta catctgaaat
    28621 ttgcatgtaa tgatcatcta tagctactgc aaaattgtac gttggaatgc catctttttt
    28681 tacgataacc cagtcaccaa taccatttga atcaaatgaa atatttcctt ttaccatatc
    28741 atcaaatgaa tacgtttggt tttgaggtac tcggaaacga attgatggtt ggcgtccttc
    28801 tgcttcaaat tgttgacgtt gttcttcagt caaatgcgca tgttgaccac catagcgagg
    28861 catttcacca cgagcgattt gcgcttcacg ttcagcttct aattcttctt ctgtcatata
    28921 gcatttatat gctttatctt ctgctagtaa ctgatctatt aatggttggt agatatgttg
    28981 acgttcagat tgacgatatg gtccgtagcc attgtcttta tctacagact catcccaatc
    29041 taatcctaac catttaagat tatcaaattg tgatgtttct ccatcttcta aattacgttt
    29101 tttatcagta tcttcaattc gaatcacaaa atctccgttg taatgtttag catacaagta
    29161 attgaataat gctgttcttg cattaccaat atgaagatac ccagttggac ttggtgcata
    29221 tcttactctt atacgatcgc tcattttttt cactcctaaa ttaaatatca gattttcaag
    29281 ttagttcata taaattgttc atttgctatc ttcgaccgtc ataacaaatg tctaactcgt
    29341 cttattgtta aaacgaaaca atgcttttta acatgacctt aaaataattt cattgtttaa
    29401 tcataacata attccctggg taatatgctt aaattttaaa tagaaagctg ttgttttttc
    29461 aacactttaa aaaagctatc cctaagaatc aagactgcac tacataaaca tttcaacgac
    29521 ttaaaaatga atattatgta ctgtgaaatt aaacttcaaa ttaactattc aaatacgtta
    29581 aaattgattc taattttgta tgtcttgatt gctataagaa taactttatt aatatctaaa
    29641 atttaacact taatgaactt gtttcaatga tatattagca ctatttgtat tttttgataa
    29701 ctaatatgtt ttgcatttat ttatagttat acttcaaatt acaaacttcg ccatttcata
    29761 taccttttaa tatctatttt gttttcgtca actacagttt ttataatgat actgtatctt
    29821 cgattttttt agcaaaaaca attcttcctg aagatgtttg caataagctg actacttcta
    29881 aattgacatg actgccaata agatttttag cattatcaac aactaccatc gtaccatcat
    29941 ctagatatcc tactgcctga ccaggctcct tacccatttt tgtcagtaaa atatgcagtt
    30001 gatcaccttg atgtacatta ggtttgattg cttctgataa atcattaaca tttaatgctt
    30061 tgataccatg tacatgacaa actttattta ggttgaaatc tgtcgttata atacttgcat
    30121 gatattgttt tgctaatttt aataacatcg tatcaatatc actatgtgtt ttagttggat
    30181 gtataacctt tgtaggatag tctaaatcat acaattcatt taaaatatct aagcctcttt
    30241 tacccttttc acgtttaaca ctgtcatttg aatctgcaac aatttgtaat tcattaataa
    30301 caccttgtgg aattaaaata ttgccatcga taaaaccgca acgaatgact tctaaaatac
    30361 gaccatcaat aattgcgctt gtgtcgataa tttttggcgt agcactttta gtatgttgtg
    30421 acatggaacg cgctatattc tcaggtaaaa acattaacat ttcatctcgt tttttaaggc
    30481 caaattggaa accgaaataa catagtaata tcgtaattat gacaggaatg aaatgattaa
    30541 aaatagagtt gccaattgat tctaatataa acgacaccat aacagaaata agtaatccga
    30601 ttattaaacc tattgttgcg aatagtattt caacagcact tctacgcata ataaaatgtt
    30661 ctaaaccttt tatagcgtta gtaactcgtc taataaatac accaaaaatt aagaacataa
    30721 aaatactacc gataatgcca tctacatagt gattttttaa aaagctggag ttttgtaatc
    30781 caagatcatt tgcaatttca ggaataataa ttattcctaa tgcgctccca ataattaagt
    30841 aaataataat aaccattagt ttaacgatat tcacacaatg tcctcctttc ttgatgtttt
    30901 atgaatgaag agcaaatgac aatacttcat gtacagtagt tacacctatt acttgtatac
    30961 cttcaggata tgtccatccg cctatattat ttttaggaat aattacacgt ttgaaaccta
    31021 gttttgcagc ctcttgcacg cgttgttcta tccgagatac acgacgtacc tcacccgtta
    31081 aaccaacttc tccaatatag caatctaatc cgtcgacagc tttatcttta aagctagatg
    31141 cagttgctac aattacactt aaatcaactg ctggctccgt taactttaca ccgccagcta
    31201 ctttgatata agcatcttgt tgttgtaata gataattttc tttcttttcc aaaacagcca
    31261 tcaacaaact taatcgatta tgatcaatcc ctgttgccat tcgtctcgga ttgttaaaag
    31321 tcgttggagt taccagcgct tgaacttcta ttaaaagtgg tctggttccc tccatggttg
    31381 caacaattgt tgaacctgga acatttgttg aacgttcttc taaaaacatt tcagatggat
    31441 tatttacacc ttttaatcca ctttgcttca tttcgaagat tcccatttca ttcgttgaac
    31501 caaaacggtt tttaacagct cgcaaaattc gatatgcgtg gtgttcatcg ccttcaaaat
    31561 aaagcacagt atcaaccatg tgttctagca atcttgggcc agcaatttga ccttctttcg
    31621 ttacatgacc cactataaaa gttgcaatgt tcatttgttt agcaatattc attaaacttt
    31681 gtgtactttc acgaacttgt gaaacagaac ctggcgcaga gctgatttca ggatgatata
    31741 ttgtttgaat cgaatccact actaataaat caggttgttc ttcttttact gtttgataaa
    31801 taacttcaag atctgtttca gctaatactt gcaattcact tgaatcttca tctaatcgct
    31861 ctgcacgtaa tttagtctga ctaagcgatt cttctccagt aatatatagt acttttttct
    31921 tttgagataa cgatgcacaa atttgtaaaa gtaacgttga cttaccaata cctggatccc
    31981 caccaataag tactaacgat ccgctcacaa tacctccacc taatacacgg ttgaattctg
    32041 ctgaatctgt taacactctc ggcgttgttt catgtttaat actatttaat ttttgtactt
    32101 tacctgctaa ttccttggtt ttaactccat gtttaggatt ggctgctttt tcaacaattt
    32161 cctccatttg attccaagcg ccacaattag gacatttccc catccattta ggagattgat
    32221 aaccacaagc catacattca aaaatcactt ttttcttggc caaaattgca cctccacttt
    32281 cctttgaaca aatctatttt aaacgcacat tcccattatg acaaattaaa tgtgagttac
    32341 atttgtttgt attttaacat gactactaac gcaaactgtt ttaccagatt tattttttat
    32401 gaatattata aaataaactt acatcatgtt gatagtcggt atctttcaaa gataaaatct
    32461 taataatttc ttagtaaact cttttctcta gatttatcac aatattatat agacctattt
    32521 tattttgacg taagttgcta gtatcttcaa acaaaaaacc tttataaaaa ttcatacctt
    32581 tatgctatcg ctgtaggctc attaacttgt tacatataat tcttaactat cctttgatga
    32641 ttgttttatt agattgtttc gttgatggat actttcacga atttctatag ttcaatgcta
    32701 ctaaaaaaac agcccaaaac tttaatttgt tttggactgt tttataatta tgcttgcgat
    32761 ggtgttttag tttctgaagt ttgttcagca atgtcatatt taaactcttt accatcatga
    32821 tctactgtaa ctttcttacc ttcaatttga ttaccatcta atattaattc acttaaatta
    32881 tcttcgatag ttttttgtat cgctctaatt aatggtcttg caccatattc tggatcatat
    32941 ccttcttctg cgattttgtc tttcgcttta tcagttacaa taatatttat gttttgttca
    33001 gataatcgat ttgttaattt attaaccatc attgttacaa tttcttttaa ttcttctttt
    33061 gttagtttat ggaatacaat gatatcatct acacggttta aaaattctgg acggaatgaa
    33121 ttttttaatt cttttaacat cgtttttcga attgtttcat aatcttgtcc atcacttgaa
    33181 ccaccgaatc cagcaaatcg ttgatcttgt aattcttgtg ccccaacgtt tgatgtcatt
    33241 atgataattg tatttctgaa atcaactgta cgtccttttg tatctgtcaa atgtccatca
    33301 tctaaaactt gtaatagaat attaaataca tctggatgag ctttttcaat ttcatcaaat
    33361 aaaattacag aatatggttt acgtctaact ttttcagtta attgtccacc atcatcatga
    33421 ccaacatatc ctggaggagc accaactaat cggctcactg cgtgtttttc cataaattca
    33481 ctcatgtcta cacggatcat cgcatcatca tcgccaaaca ttgattcagc taaagctcta
    33541 gctaattcag ttttaccaac accagttggt ccaaggaaga taaagctacc aattggtcgt
    33601 ttaggatctt ttaaccctgc acgggcacgt ctaaccgctt tactgattga attaacagca
    33661 tctttttgcc caataactct ctcatgtaat gtatcttcta gactaagaag tttttcagat
    33721 tctgtttcat tgattttagt taatgggata cctgtccatc ctgcaataac ttcagcaata
    33781 tcttcttctg acaatgaagt tgacatgcca ttttgtgcat tcttccattc atttttagct
    33841 tcttcatatt gcttttcaag ttttgtttgt ttatcacgca ggttagcagc attttcaaac
    33901 tcttgagcat gtactgcggc atctttttca tttttaactt tttcaatttc ttgttcaatt
    33961 tcttttaaat tattaggtgt cgtatgactc ttaagtctta ctttagaact tgcttcatca
    34021 attaaatcaa ttgctttatc tggtaagaaa cgatctgaaa cgtatctgtt acttaattta
    34081 acagctgctt caatagcttc gtctgaaata ttaatacgat ggtgtgcttc gtaacgatct
    34141 cttaatcctt ttaaaatagc aactgtatct actactgaag gttcatcaac ttgtacaggt
    34201 tggaaacgac gttctaaagc cgcgtctttt tcaatatttt tgcgatattc atctaatgta
    34261 gtagcaccaa tacattgtaa ttcaccacgt gctaatgccg gcttcaaaat attcgaagca
    34321 tcgatagcac cttcagcacc accagcacca actaaagtat gcaactcatc aataaatagg
    34381 atgacattac ctgcttgttg gatttcttcc ataacctttt tcagacgctc ttcaaattca
    34441 ccacgatatt tagtacctgc aactactgtt cccatatcta aagacataac acgcttatct
    34501 tttaatgtct ctggtacctc attattcact atggcttgcg ctaaaccttc agcaatagca
    34561 gttttaccaa cacctggctc tccaataagc acaggattgt ttttcgtacg tctacttaat
    34621 acttcaatta cacgtgtaat ttctttatca cgtcctataa caggatctaa tgtaccgtct
    34681 ttggcaatga ctgttaagtc acgagctaaa ctatctaaag ttggagtatt atttgactta
    34741 ctagcttgtg catttttatt actcatttca gggtttccta aagctttcac aacttgtgca
    34801 cgtgctttag taatatttaa atctagattt gcaaaaactc ttgctgcaac accttcattt
    34861 tcacgaatca agcctaataa aatatgttcc gttccaacaa aattgtgatg taattttcta
    34921 gcttcatcca tcgataattc aatgactttt ttagctctag gtgtataatg caatgtacca
    34981 acatgatctt gaccatgtcc gattaatttt tcaacttctt caattacttt atcttcagtg
    35041 atattaaaac tttctaatac ttttgcagca attccttcag gttctttcat taaccccaat
    35101 aataggtgtt ctgttcctat atttgaatga tttaaacgaa ttgcttcttc ttgggcatgt
    35161 gctaatacgc gctgtgcacg ctcagttaat ctaccaaata acataaataa tgacctccta
    35221 ctttatatgt tctcttagta tatctgctcg tttttctttt acagatttgt catcttcttc
    35281 atctaataaa aatggtgact gtatagctac catcaattca ttaaatttaa agttttgtaa
    35341 ttcaatgtaa tttaaatcta taccaagttt aacttcgctt aatctataag aagcctcttc
    35401 catagttatc attctacagt tttgtaaaat acctagcgag cgaaaaacac ggtcttgtgt
    35461 ttctaattga ttataagtgt ctaacttttg tcgtatttgt ttttcttcat gaatgatttg
    35521 attaacaact tctgttaatg tttctatgat ttctaactca gatttaccaa gtgtaagttg
    35581 gttggatact tgataagtat gtccataaac ttgcgaacct tcaccgtaaa tacctctgat
    35641 tgtatatcca aaacgattaa tggtttgagc aatccgtgtc attcttttca taatagatag
    35701 acctggcaaa tgtagcatca cgcttgctct cataccagta cctatattgg taggacatgt
    35761 agttaaataa ccaagttgtt catcataact tatatcaagg cttcgatcta attcatcatc
    35821 aattgatgaa gcttgattat ataaagcctg taatgtcgtg tcagttccca tagcttgaat
    35881 acgaatatgg tcctcttcat ttatcatgac acttaaagat tcatcgtcat tcactaatac
    35941 tgcggctgct ggttgtttta ttagttcagg actaatcaaa tgctttgcaa ccattttcat
    36001 tttactttgt tgatccattt gatcaagacg cattaattca aagtttggca aggcatcttg
    36061 tacctcattt ataactctaa atccatcatt ttcagtagca tacattagtg gatgcacatg
    36121 attttctaaa ttacgcgcta accgaattct agaagacata acaattggtg tttcttcatt
    36181 acttttcatc cattggctga tattatcatg aatattatgc gtcatcatgt tgcacctcac
    36241 tctcagcttt tagtgcttta atttcatctc taacaatggc tgcttcctca aaatcttgga
    36301 tttcaataag ttttttcaaa tattcattct tttcttcgat ttttcgcttt aaagctatct
    36361 ttttatgtga agaatgtggt gtctttccaa cgtgctcaaa ttgtccacct tgaactctgc
    36421 ggacgatatc aatgatgtca tctttaaatg ttgcataaca attagcacac ccaaatttac
    36481 caacatgtgc aatatctttc aaagtcatat gacatgatgg gcaacgtttt tcttctttaa
    36541 acgctacttc ttgaaaatta attccatgtt tcgtagctaa atgttgtaaa atttgcttaa
    36601 caacaaatgc ttcttcgaaa ttatcttgat gttcttgata ttcaggttgt tcattagctt
    36661 gattccacgg atggtgtccc tcagcacaag tttgacacac cattttttct tctgttttat
    36721 ttttacttgt aactttaact tttaattccg cttcattaag ttgacaattt tcacaaagca
    36781 cttcaacacc tcatttcatt tagtaataat ttataactgg taacaaacgt tttaaaatat
    36841 ttgctctaat aatatctcta gaaaccatat ccattgatag cgtttctcta tcaataactg
    36901 cttgaatcat tttagcttca cgttcattta ttaacatttt atctaaaagc ccatcaataa
    36961 tataataagc ttgttgttga gaaatagaag gtccaatcag ctgaagcaaa tgattaatat
    37021 aacctgttgc atctttattt tcaattttag tgattcggat gtaaccacca ccaccacgtt
    37081 tactttcgat ttcataacca tgttcattag tgaatcgtgt tttgattaca taatttaatt
    37141 gtgatggtac gcaatcaaaa cgctgtgcga tattcgctct ctgaatttca acgacatctt
    37201 cattcgactc ttcaaataaa cgtttgatgt attgttctat gatgtcagac atattgtgca
    37261 tgtatatcac ccctttttga ccttctttga ctatattata tgacctattt tgaccttttt
    37321 caattaattt gtttttatat tttcaaaaat ttatgatgtt agcccttcca ttttattgta
    37381 tgatagattg tagaaaaaac taaaggaatg agaaatatat gcatattcta attgggatta
    37441 tcggaattat cttcttttta gcacttgcgt ttctatttag ctcagacagg aaaaatattc
    37501 gctggaaata tgtaggaatt ttacttgcaa ttcagcttat ctttgcattt atcttgctta
    37561 aaactacaat gggaatcaag gttattggtg gtatttcaaa cggttttaac tacttattat
    37621 taaaagcagc agatggtgtt aactttgtat tcggtggtat tcaatatatc gatcctaagc
    37681 aaccaccatt cttcttcagt gtattattac ctatcgtatt tatttcagcg attatcggta
    37741 tccttcaata cacaaagatt ttacctttaa tcattaatgt tttaggattc ttaatttcta
    37801 aaattaatgg tatgggacgt ttagaatcat acaatgcagt tgctgcagca attttgggac
    37861 aatctgaagt atttatttca attaaaaaac aattaccata catacctaag caacgtttat
    37921 acacattaac agcttcagca atgtcgactg tttctgcttc aattatcggt gcatacttta
    37981 ccttaattga acctaaatat gttgtaacgg ctgttgtctt aaacttattt ggtggtttca
    38041 tcatcgcttc aattattaac ccttataaag ttaatgaaga agatgacaaa ttattagttg
    38101 aagaaactga agaaagacaa caatcattct tcgaaatgct aggagaatat attttagatg
    38161 gatttaaagt agctgtcatt gtaggcgcaa tgttaatcgg atacattgcc attatcgctc
    38221 tattaaatgg aattgtaagc aatatcttta gtacagtttc aggtggcgct atttcttggg
    38281 atttccaaac attaattggt tttgtctttg caccatttgc attcttagtc ggtgtgccat
    38341 ggcaagatgc agttcaagcc ggttcagtta tggcgacaaa actattatct aacgaatttg
    38401 tagcaatgca agcacttggt aaacttagtg atttatctga acacgcaaaa ggtgttacat
    38461 ctgtattctt agtatcattt gctaacttta gctctatcgg tattatttca ggtgcgatta
    38521 aatcacttaa tgacaaaaaa ggagatacag ttgcccgctt tggtttgaaa ttattattcg
    38581 gtgcaacact agtatctttc atttcagctg caatcgctgg attctttatt taacaaatac
    38641 atcaacatcg aattaacccc ttcaaaattt tgactattct cagtctcttt gaagggtttt
    38701 tttcttatgt atagctttat tttttatcat ttttaatttt gacataaaaa ataactcctc
    38761 caaagctaag tttgaaggag atattttctt ctcaacaaat ctataaatat caataattcg
    38821 ttgatttagc atacattaag ctatgctttt tttacaatat gattaataaa gtaatcagtt
    38881 actctatagt catctgttaa ttcaggatgg aatgatacgc ctaaatattt accttgctga
    38941 acagctacaa ttttctcatt aaccttacat aggatatcta cgccttgacc tactttttca
    39001 atatgtgggg ctcttataaa gacaccttca atatctgtag cgataccttt aatatctaat
    39061 tctgtttcaa agctgtcaac ttgtctaccg aatgagtttc gttgtacagt aatattcaac
    39121 ttgttaaggt atccttcttc accaactata tcttgcgcta gaactattaa tcctgcgcat
    39181 gtaccaaaca taggtaaagt tgaattttgt aaagcctctt taaatccata taaattcatt
    39241 aatcgacgta acgttgtaga ctcgccacca ggtaatatta agccctcgat ttcttctaat
    39301 tgttcaactt ttttaactgc aataccttca tgaccactta attcaatatg tctaatatgt
    39361 tcacgtactg caccttgtaa tgctaataca cctattttca tatcttacca accacgctct
    39421 tgcatacgtt cttctaatga taattgattg atatctaaac ctttcatagc agtgccaagt
    39481 tcacttgcta atcttccaat tagttcatag tcttggtaat gtgttgttgc ttgaacaatt
    39541 gctttagcaa atttttctgg atcttctgat ttaaaaatac ctgatccaac gaatacaccg
    39601 tcagcaccta attccatcat taaagcagca tcttgaggag tcgcaacgcc accagctgca
    39661 aagttaacta ccggtaaacg accattgtct ttaatttgtt ttaaaatttc ataaggcgca
    39721 ccgatatctt tcgcaaaagt cataatctca tcatcattca ttacagtcaa tcgactaact
    39781 tctgaattaa cttgtctcat atgtcttaca gcttcaacaa tattacctgt acctggttca
    39841 cctttagtac gtaacatagc agcaccttca ccaattctac gcgcagcttc acctaaatta
    39901 cgacatccac atacaaatgg tactgtaaat tgatcttttc ttaagtgata ttcctcatct
    39961 gctggtgtta acacttctga ttcatcaata tagtcaacac ccatcgcctc taatactctt
    40021 gcttcagtga tatgaccaat acgtgcttta gccatgactg gaatagaaac agcattcatt
    40081 acttcttcta caattttagg gtttgccata cgtgcaacac caccagcagc tctaatatca
    40141 gaaggtactc gttctaatgc cataactgct accgcgccag cttcttctgc aattcttgct
    40201 tgctcagcat taacgacatc cataataacg ccgccttttt gcatttcagc catacctctt
    40261 ttgactctgt ctgatccaat aattttactc attatacata aaaccccttt tcaagttgat
    40321 taaatacatt gtaatgtata aactgatatt taaaaaggta caaaataaga taaatttagg
    40381 aggtcagtta taaaattgaa aaacacttta taccatcaac tttatgaaaa attaaaaaaa
    40441 caaataattg aaggtcaatt taaagaaggc gataaatttt attctaaaag acaattaagc
    40501 aaacatttat ctattagcca aacaaccgta gagcatgcat atcaacttct attagacgaa
    40561 ggttacattt attcaagacc tcgctctgga tattttgtgt ccgaaattga atctttaaca
    40621 attttaaaca atcaacctat cccctctctt tttgatgatg attcatataa accaaaagcg
    40681 agtgatgaag cttatgatta tgcttttaat ttggatgaaa tagatacgaa acatttccct
    40741 atagaacttt tcagaaagta ttccaaagat ctttatgata caaatcattt aaatcaatta
    40801 cgtagaggac attttcaagg agaattacat ttgcggtttc aactggcatt ttatttattt
    40861 actaatcgtg gcgtaatttg tgatcctaat caaattataa ttggctcatc taccgaacaa
    40921 ttagtaaatc aattagtcga tttactttat acttcaacat ttattatcga aaaacctagt
    40981 tatccaccaa tcaaaaatat tttagataaa aaacaagttg aatatgaaca aattgaagtt
    41041 gaagataatg gaataaacgt agatgaagtt attaaatcac aaaaaaatat agtatatata
    41101 acgccttccc atcaatttcc aactggatat gtgatggact taaaaaaacg aactcaatta
    41161 atacaatggg ctcaagaaaa agaagaacga tttattatag aagatgatta tgattccgag
    41221 tttagatatt tcggcaaacc cattccagca attcaaggtc tatattcaag aggagaaaaa
    41281 gtgatttata tcagtacatt ttcaaagtct atttttccta gctgtcgagt tgcatatatg
    41341 gtgttaccct attctataat gaaaaaatat cattctcaaa atcacattga aggcaacact
    41401 gtacctgttc atatgcaaaa ccttattgca acgtttatat cttccggtgg ttttgaaagg
    41461 catttaaata aaatgagacg aatttatcgt agaaaactaa cctatatttt aaaaagattg
    41521 aaaccttaca aagagcagct tgatattcaa ggtgctgaaa cgggtatgca ttttacaatt
    41581 acagtaaaaa atggattaac attacaagag tgtcttgata gagcaaacaa agtaaagtta
    41641 aaattacaag tttataattt tgatgatgat caagactata agaaaacccc taaatttata
    41701 ttaggctttg gcggtataga cgatgatgct ttaaaaccgc atgtagacgc tctaataaaa
    41761 tcattaatta taaagtaaca cttctttgat aaaagttagt gttacttatg caatagcttg
    41821 t
//
LOCUS       DANRDI010000006        41037 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.006, whole genome shotgun sequence.
ACCESSION   DANRDI010000006 DANRDI010000000
VERSION     DANRDI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 41037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.006"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..545
                     /locus_tag="PZ377_000181"
     CDS             <1..545
                     /locus_tag="PZ377_000181"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDM6357074.1"
                     /translation="TWSSGIKSPIYCDNRVTLGYPLVRGAIRDGLINLIKEHFPEVEV
                     ISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGKQNQIEGAKSEGKKVVVIEDLIST
                     GGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDTFSNIQLPFYTLSDYNELIEVAEN
                     EGKISSEDIQTLVEWRDNLA"
     gene            575..787
                     /locus_tag="PZ377_000182"
     CDS             575..787
                     /locus_tag="PZ377_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357075.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            1224..1625
                     /locus_tag="PZ377_000183"
     CDS             1224..1625
                     /locus_tag="PZ377_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6357076.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(1888..3585)
                     /locus_tag="PZ377_000184"
     CDS             complement(1888..3585)
                     /locus_tag="PZ377_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDM6357077.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            3860..4483
                     /gene="gmk"
                     /locus_tag="PZ377_000185"
     CDS             3860..4483
                     /gene="gmk"
                     /locus_tag="PZ377_000185"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDM6357078.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            4483..4701
                     /locus_tag="PZ377_000186"
     CDS             4483..4701
                     /locus_tag="PZ377_000186"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDM6357079.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            4917..6116
                     /gene="coaBC"
                     /locus_tag="PZ377_000187"
     CDS             4917..6116
                     /gene="coaBC"
                     /locus_tag="PZ377_000187"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDM6357080.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            6116..8524
                     /gene="priA"
                     /locus_tag="PZ377_000188"
     CDS             6116..8524
                     /gene="priA"
                     /locus_tag="PZ377_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDM6357081.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
     gene            9030..9983
                     /locus_tag="PZ377_000189"
     CDS             9030..9983
                     /locus_tag="PZ377_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357082.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(10146..10349)
                     /locus_tag="PZ377_000190"
     CDS             complement(10146..10349)
                     /locus_tag="PZ377_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDM6357083.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            10648..11136
                     /locus_tag="PZ377_000191"
     CDS             10648..11136
                     /locus_tag="PZ377_000191"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDM6357084.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            11129..12064
                     /locus_tag="PZ377_000192"
     CDS             11129..12064
                     /locus_tag="PZ377_000192"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDM6357085.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            12061..13368
                     /gene="rsmB"
                     /locus_tag="PZ377_000193"
     CDS             12061..13368
                     /gene="rsmB"
                     /locus_tag="PZ377_000193"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDM6357086.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            13371..14465
                     /gene="rlmN"
                     /locus_tag="PZ377_000194"
     CDS             13371..14465
                     /gene="rlmN"
                     /locus_tag="PZ377_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDM6357087.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTTRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            14472..15215
                     /locus_tag="PZ377_000195"
     CDS             14472..15215
                     /locus_tag="PZ377_000195"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDM6357088.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            15212..17206
                     /gene="pknB"
                     /locus_tag="PZ377_000196"
     CDS             15212..17206
                     /gene="pknB"
                     /locus_tag="PZ377_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDM6357089.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            17434..18309
                     /gene="rsgA"
                     /locus_tag="PZ377_000197"
     CDS             17434..18309
                     /gene="rsgA"
                     /locus_tag="PZ377_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDM6357090.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            18310..18954
                     /locus_tag="PZ377_000198"
     CDS             18310..18954
                     /locus_tag="PZ377_000198"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDM6357091.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            18961..19602
                     /locus_tag="PZ377_000199"
     CDS             18961..19602
                     /locus_tag="PZ377_000199"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDM6357092.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(19983..20171)
                     /locus_tag="PZ377_000200"
     CDS             complement(19983..20171)
                     /locus_tag="PZ377_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDM6357093.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            20614..20988
                     /locus_tag="PZ377_000201"
     CDS             20614..20988
                     /locus_tag="PZ377_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDM6357094.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            21003..22649
                     /gene="fakA"
                     /locus_tag="PZ377_000202"
     CDS             21003..22649
                     /gene="fakA"
                     /locus_tag="PZ377_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDM6357095.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            22839..24899
                     /gene="recG"
                     /locus_tag="PZ377_000203"
     CDS             22839..24899
                     /gene="recG"
                     /locus_tag="PZ377_000203"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDM6357096.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            25102..25674
                     /gene="fapR"
                     /locus_tag="PZ377_000204"
     CDS             25102..25674
                     /gene="fapR"
                     /locus_tag="PZ377_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDM6357097.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            25679..26665
                     /gene="plsX"
                     /locus_tag="PZ377_000205"
     CDS             25679..26665
                     /gene="plsX"
                     /locus_tag="PZ377_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDM6357098.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            26658..27584
                     /gene="fabD"
                     /locus_tag="PZ377_000206"
     CDS             26658..27584
                     /gene="fabD"
                     /locus_tag="PZ377_000206"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDM6357099.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            27577..28311
                     /gene="fabG"
                     /locus_tag="PZ377_000207"
     CDS             27577..28311
                     /gene="fabG"
                     /locus_tag="PZ377_000207"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDM6357100.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            28554..28787
                     /locus_tag="PZ377_000208"
     CDS             28554..28787
                     /locus_tag="PZ377_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDM6357101.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            28903..29634
                     /locus_tag="PZ377_000209"
     CDS             28903..29634
                     /locus_tag="PZ377_000209"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDM6357102.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            29781..33347
                     /gene="smc"
                     /locus_tag="PZ377_000210"
     CDS             29781..33347
                     /gene="smc"
                     /locus_tag="PZ377_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDM6357103.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            33347..34597
                     /gene="ftsY"
                     /locus_tag="PZ377_000211"
     CDS             33347..34597
                     /gene="ftsY"
                     /locus_tag="PZ377_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDM6357104.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            34584..34916
                     /locus_tag="PZ377_000212"
     CDS             34584..34916
                     /locus_tag="PZ377_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDM6357105.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            34942..36309
                     /gene="ffh"
                     /locus_tag="PZ377_000213"
     CDS             34942..36309
                     /gene="ffh"
                     /locus_tag="PZ377_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDM6357106.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            36744..37019
                     /gene="rpsP"
                     /locus_tag="PZ377_000214"
     CDS             36744..37019
                     /gene="rpsP"
                     /locus_tag="PZ377_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDM6357107.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            37207..37710
                     /gene="rimM"
                     /locus_tag="PZ377_000215"
     CDS             37207..37710
                     /gene="rimM"
                     /locus_tag="PZ377_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDM6357108.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            37710..38447
                     /gene="trmD"
                     /locus_tag="PZ377_000216"
     CDS             37710..38447
                     /gene="trmD"
                     /locus_tag="PZ377_000216"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDM6357109.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            38550..38900
                     /gene="rplS"
                     /locus_tag="PZ377_000217"
     CDS             38550..38900
                     /gene="rplS"
                     /locus_tag="PZ377_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDM6357110.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(39144..>41037)
                     /gene="yfhO"
                     /locus_tag="PZ377_000218"
     CDS             complement(39144..>41037)
                     /gene="yfhO"
                     /locus_tag="PZ377_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDM6357111.1"
                     /translation="VSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRKQNPNVD
                     IPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILFIGSLSQ
                     YFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCIIAILYV
                     LLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILDNNKNMA
                     IKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFIYHYNGI
                     SLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRVNHDDNL
                     PYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAMLQGIVSN
                     NIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKSVSNQFK
                     DLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRIRLSLPK
                     GKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLPTAYNQG
                     MKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGIICSIIFT
                     RWARQK"
BASE COUNT        15056 a         5613 c         7771 g        12597 t
ORIGIN      
        1 atacatggag ttcaggtatt aaatcaccga tttactgtga taaccgtgtt acgttaggtt
       61 atcctttagt tcgaggcgca atccgcgatg gtttaattaa cttaattaaa gaacactttc
      121 ctgaagtaga agttatttct ggtactgcaa cagctggtat tccacatgca gcttttattg
      181 ctgaaaaatt aaaattacca atgaattatg ttcgttcatc aaataagagt catggtaagc
      241 aaaatcaaat cgaaggtgct aaaagtgaag gtaaaaaagt agttgtgata gaagatttaa
      301 tttcgacagg gggatcttca gtcacagcag ttgaagcctt aaaactagca ggtgcagaag
      361 tattaggtgt tgtagctatc tttacttacg gtttgaaaaa agcagatgat acatttagca
      421 atattcaact acctttttac actttaagtg attacaatga attaattgaa gtagctgaaa
      481 atgaaggtaa aatttctagt gaagatatcc aaacattagt tgaatggaga gacaacttag
      541 cataatatag acactagaag gaggaattca acaaatgaat gacaaaacat ctaatgattt
      601 atatgggaag ataaaacatt gtaacgaatt tatcaatcat tcaaatgatt ccaatctatc
      661 tagtagtcac gatgtcgacg aaagttcaac gaagcaaaaa catataaaaa ataaaacaac
      721 tatagatcat aatgatgatt tatttaaaca tgtaaaggat atattacgta aacaagaaca
      781 aatttaaaaa caaaaaagct atagtgttta atatcagggg ctgcttaacc gcatccgaat
      841 attacaaaca ctatagcttt tattatataa aaaatttatt agcggataat taactttatt
      901 aaaatccaac caattaagaa tacgagtaat aggactaata ctggaatcac ataatgtaac
      961 ataacgtccc tcctttaact taattttaat tgtaatcaaa tttgacaata agtcaaaaca
     1021 ttaataccta tgataagtat catttattaa catatgtatc atatttttaa tcttgcgtaa
     1081 tttttatcgt taactatgtg atttaatcaa caggccaata agacgtttgt cattcgtgca
     1141 cataacggta cgacgcgctt ttgtcatttt aattatgtta agataatagt agttatagaa
     1201 gttcaatcta taggaggcat agcatggata ttccaaaaat cacgacattt ttaatgttta
     1261 ataaccaagc tgaagaagct gttaaactat acacaagctt atttgaagat agtgagatta
     1321 taacaatggc taagtatggt gaaaatggac ctggtgatcc cgggactgta caacactcaa
     1381 tatttacatt aaatggacaa gtattcatgg cgattgatgc taatagtggc acagaattac
     1441 caatgaatcc tgcgatttca ttatttgtta cagtaaaaga tactattgaa atggaacgac
     1501 tatttaatgg attaaaagat gaaggtgcca ttttaatgcc aaaaacgaat atgccaccat
     1561 acagagagtt tgcttgggtt caagataagt ttggagtaag ttttcaatta gcattacctg
     1621 agtaaaagga ttcgcacagc gttgaatgat aaagaaacac tttttcttat gcatgcgttt
     1681 tacctatgta tttctatttt aagtacacat tagcatttta gtttcactca tttttaaaat
     1741 cacaaagata atttgtaact taaatgatga tacgttattt aaaaacacga tacttcgttt
     1801 caatgaacgc attaaataat aaataaacac ctcaccataa gaaaggacta ctttcttttg
     1861 tgaggtgttt atttgtcgac aacttgatta tgattgtttc attttttgaa taagttcata
     1921 atcaggtgta gcatacaaag ttttttgatt gtcatatgtt acaaacccag gctttgcacc
     1981 tgatggttta tgcacatttt taattaatgt ataatcaaca ggtatttgtc cagaattacc
     2041 agcttttgaa aagtatcctg ctaacatagc cgcttccttg attgtcgtat cacttggtgc
     2101 atcattaaat ataacgacat gtgaaccagg aatatctttt gtgtgtaacc atgtgtgagt
     2161 ttttttagct tttttatttg ttaaataatc attttgcttg ttattcttac caacatatat
     2221 atcgtcgcca tcagttgata cataatgttg taattgaatc tgcgctttct ttttcttagt
     2281 ttgattttta cgctgtttca taaagccttg ttctgctaat tcatctctaa tttcatcaat
     2341 gtcatggaca gaaatatgat gtaattgttg ttcgattgtt gaaaaataat ctatattgtc
     2401 tttcgtcaat tgaatttgat gttgtaattc acgttctctc gttttcatac gattatattg
     2461 tttataataa tattgagcat ttgctgatgg ggattttgta ggatttaaag gaatgacaac
     2521 ttcttcattc gtataataat tcaatgccgt cacttcttta tcgccttgct taattcgata
     2581 tatattagca gtgatcaatt caccatataa ctgttcagta tctttatttt tagactgctc
     2641 atattcttca atcaacttcg ctaatttatt ttgatatttg tacaactgct gttgaacaaa
     2701 tcgaactaaa tcattcgcac gttgtttaac gcgttcacgt tcgccacgcg catcataaaa
     2761 acgatcaagc aaatcattta atgaatcgta tgtaactgta tcatcattaa attgatttaa
     2821 ctttataaaa tagaaatcct ctttacctgt ttcatgattt ttatgaaaaa taggagtagg
     2881 tggtaacttg gtttctgcca ttacttcgtc aaatgcttct ggtaatgttg atgaagtaat
     2941 aaattgacga cgactaacga tttcattcgt aattaaaggg ctaaatcctt caaactgatt
     3001 caataattgt ttagcaatat tacctgcgtt aaaatcgata tatttcaaca cctctgcacc
     3061 tgtaatatca tacggattta ttttgtgctg agtaggtggt gcttcataat taaatcctgg
     3121 cattactgta cgatagtgat tcgtatttgg tgttaagtgt ttaaatcctt caattatttt
     3181 gcgattttca tctactaaaa ttaagttact atgtttaccc ataatctcaa ggatgacagt
     3241 gcggtaaata gtatcgccaa tttcatcttt actctttata tcgatttcaa tgcgacgatc
     3301 attaccaatt tgcttaatcg attcgataat accaccttct aagtgttttc taaaaacacg
     3361 cgcaaacatg ggtggattaa atggattatc atatttttta gtagtcaatt gtaatcttga
     3421 aaagtttgga tggattgaca ataacaattg atggttttgt ctattttgac gtacaaccat
     3481 tagtatcgtg tcattatcag gttgattgat tttgtgaacg cgtcctgttg ttaaaaattg
     3541 tagagactca accattttct ttgtaaataa gccatcataa gccataattt atcttccacc
     3601 ttcttcatat cattcgtttc attttaacat gattgtaact tttcagtata gttattgaac
     3661 catacattat gccatacatt ttacattatc taaaagtttg caattcaatt tatttaatta
     3721 aaattaatcg tgcaatataa cgatattgtt agacttaata gaaattatgg catgcaattt
     3781 caaatatgct atacaatata aagaacaatg tgatatcata tttaaataat agaagattag
     3841 cttagagagg tcgtaaggca tggataatga aaaaggattg ttaatcgttt tatcaggacc
     3901 atctggagta ggtaaaggta ctgttagaaa acgaatattt gaagatccaa gtacatcata
     3961 taagtattct atttcaatga caacacgtca aatgcgtgaa ggtgaagttg atggcgtaga
     4021 ttactttttt aaaactaggg atgcgtttga agctttaatc aaagatgacc aatttataga
     4081 atatgctgaa tatgtaggca actattatgg tacaccagtt caatatgtta aagatacaat
     4141 ggacgaaggt catgatgtat ttttagaaat tgaagtagaa ggtgcaaagc aagttagaaa
     4201 gaaatttcca gatgcgctat ttattttctt agcacctcca agtttagaac acttgagaga
     4261 gcgattagta ggtagaggaa cagaatctga tgagaaaata caaagtcgta ttaacgaagc
     4321 gcgtaaagaa gttgaaatga tgaatttata cgattacgtt gtagttaatg atgaagtaga
     4381 acttgcgaag aatagaattc aatgtattgt agaagctgag cacttaaaaa gagagcgcgt
     4441 agaagctaag tatagaaaaa tgattttgga ggctaaaaaa taatgttaaa tccaccttta
     4501 aaccaattaa cgtcacaaat taaatcaaag tatttaattg caacaactgc agcgaaaaga
     4561 gcgcgtgaaa ttgatgaaca acctgaaact gaattattaa gtgaatatca ttcatttaaa
     4621 ccagttggta gagcgttaga agaaattgct gacggtaaaa ttcgccctgt tatttcaagt
     4681 gattattatg gtaaagaata gtttcgacca ttaaaaatat gtgaagctag acgtacatca
     4741 aatacgtcaa ggcatatcca aatcaaacgc acctatgtag gtgtgttttt ttagtttatc
     4801 caatgattga tgttataata atactaaatt tgtatctata aaaaagtaat gagcatttgt
     4861 gcgcatatga tgatgtaaag cgtaaatgtc atagtgatat ttaagggaga ttgaatatga
     4921 agaaaatatt attagccgtt acaggtggca ttgcggcata taaagcaatt gatttgacaa
     4981 gtaagttaac acaatctggg tatgaagttc gcgttatgtt aacgaatcac gcacaaaaat
     5041 ttgtgacacc attagcattt caagcaataa gtcgaaatgc tgtttataca gatactttta
     5101 tagaagaaaa tccttcagaa atacagcata ttgcattagg tgattgggca gatgcaatca
     5161 ttgttgcacc tgcaacggca aatacaattg caaaattgag tgtaggtatt gctgatgatt
     5221 tggtgacatc aacgttgcta gcaacagaga caccgaaatt tattgcgcct gctatgaatg
     5281 tgcatatgta tgaaaataaa cgtacgcagc aaaatattaa tattttaaaa gaagatgggt
     5341 atcattttat cgaaccagga agcggatttc tagcatgtgg ttatgttgct aaaggacgta
     5401 tggaagaacc gcttcaaatc gtttctgtaa ttgatgctca ttttcaaaat agtaatcgtt
     5461 tagctaatag ttcatttcaa gataaacgcg cattggttac agcaggacca actattgaag
     5521 ttatcgatcc agtcagattt gtatccaatc gttcttctgg aaaaatgggc tatgcaatag
     5581 ctgaagcatt gcgaaatcga ggagctatcg tgacgttagt tgctggtcct acaacactag
     5641 aggatccaaa agatattgaa gttattcatg ttcaaagtgc tgaagaaatg tttgaacaag
     5701 tgacaagccg atttgacgaa caagatattg ttgtaaaagc agcagccgta tctgactata
     5761 caccggttga tgtattagaa cataagatga aaaagcagga tggtgatttg tcagtatctt
     5821 ttaaacgtac taaagacatt cttaaatatt taggtgaaca taaaacatca cagtatttaa
     5881 taggctttgc agcagagact gaagatattg aaaattatgc acaacaaaaa ttacgcaaga
     5941 aaaatgcaga tgtgattatt tcaaataatg ttggggatat gtctatcgga tttagttctg
     6001 atgataatga attgacaatg cattttaaaa ataatgaaaa agtaaatatc aagaaaggaa
     6061 aaaaagtagt attagctgca caaattttag atgaactaga aactaggtgg caataatgat
     6121 agcgaaagtc atagtcgatg tcgcgtcgaa gagcgttgac tataaatttg attatataat
     6181 tcccgaacaa ctcgaatctg tcatccaacc tggtgtgcgt gtaattgtac cttttggacc
     6241 aagaacgatt caaggttatg taatggaagt aacagcagaa cctgatgcac aacttgacgt
     6301 ttcgaagtta aaaaaaatca tagaagtgaa agatatacaa ccagaattaa catcagaatt
     6361 aatagcttta agtgagtgga tgggttcaac tcatgtcatt aaacgtattt ctatgctaga
     6421 agtgatgctt ccgagtgcta ttaaagcgaa gtataaaaaa gcatttaaga tgaaagatga
     6481 catagagcta ccttcagctt tattacaaaa atttgataag catggttact attattataa
     6541 agatgcgcaa aaaaataatg atattcaatt gcttatgaag ttgttaaaag atgatatcgt
     6601 tgaagaaaaa acgattctca cacaaaatat aactaaaaaa accaagcgtg ctgttcgtgt
     6661 cattgaaggg tatcatcctg atgaagtatt agctaagttg gagaaagtta ttaaacaata
     6721 cgatttgtat gcttacttgt ctgaagaaca acataaaaca atatttttaa ctgatattga
     6781 ggatatgggc ttttcaaaat ccagtttaga tggacttatc aaaaaaggtt atgttgaaaa
     6841 atatgacgcg gttgttgaaa gagacccatt taaagatcgt gttttcgaac aagaatcaaa
     6901 acagcaatta acagaagacc aatataaagc atatgaagcg attaaagcta aaattgtaag
     6961 ccaagagcaa gaaacatttt tacttcatgg tgtgacggga tcaggtaaaa cagaagtata
     7021 tttacaaacg atagaagatg ttttaagcca aggaaaacag gcgatgatgt tagttcctga
     7081 aatcgctcta acaccgcaaa tggttttacg cttcaaacgt cgatttggtg atgacgttgc
     7141 tgtattacat tctggcttat ctaatgggga acgttatgat gaatggcaaa aaattaggga
     7201 tggtcgtgcg agagtaagtg ttggtgcaag gtcaagtgtg ttcgcacctt tcaaaaattt
     7261 agggttaatc atcattgatg aagaacatga atctacatat aaacaagaag attatccgag
     7321 atatcacgct agagaaattg cccaatggcg aagtgaatat catcactgtc cagtcatttt
     7381 aggaagtgca acaccatgtc ttgaaagtta tgcacgagct gaaaaaggcg tttatcattt
     7441 gctatcatta ccaaacagag tgaaccaaca agctttacct gaaattgata tagtagacat
     7501 gcgtgaagaa ttgagtgaag gtaatcggtc aatgttttca aaagatttac gtgaagccat
     7561 acaattaaga ttagatcgac aggaacaagt tgttttattt ttaaatcgac gtggttatgc
     7621 atcgtttatg ttatgtcggg attgtggata tgtaccgcaa tgtccaaact gtgatatttc
     7681 attaacgtat cataaaacga cagacttatt aaaatgtcac tattgtggtt accaagagac
     7741 gccaccgaat caatgtccaa attgtgagag tgaacacatt cgacaagtag gtactggtac
     7801 tcagaaagtt gaagaactat tgcaacaaga atttgaagat gcgcgcataa ttaggatgga
     7861 tgtagataca acctcaaaga aaggtgcaca tgaaaagtta ttgactgaat tcgaaaaagg
     7921 taacggtgac attttactag gtactcagat gattgcgaaa ggattagatt atccaaatat
     7981 tactttagtt ggtgtgctga atgcagatac aatgttaaat ttacctgatt ttcgggcgag
     8041 cgaacgtact tatcaactat taacgcaagt ggctggtaga gctggtcgtc atgaaaaggc
     8101 aggtcaagtc atcattcaaa cgtataatcc agatcattat tcaatattgg atgttcaaaa
     8161 aaatgattat ttaacatttt atcgtcagga aatggaatat cgtaaattag gaaagtatcc
     8221 accgtattat tatttgatta atttcacaat ctcacataaa gaaatgaaga aggttatggt
     8281 agcatcgcag catgttcata aaattttatt acagcattta acagaaaaag cgcttgtact
     8341 aggtccatct ccggcagcac ttgcgagaat caacaatgaa tttagattcc aaattttagt
     8401 gaaatataaa agtgaacctg gattattaca agccattcag tttttagatg actattacca
     8461 tgaaaaattt ataaaagaaa aattagcatt gaagattgat attgatccac agatgatgat
     8521 gtaacattac taattattag aaacaagttc agtatgtacg agtatttgaa ccagtgtttg
     8581 aatatttact ttaagtacag aaaaagggca gaatatacaa ctgttaacta tttaaattag
     8641 cagtttatat tctgtctttt tatatggctt tataacttac gtgattttgg tttgataagg
     8701 aatttattag tattttcatt tacgaattct gattttaatg caagcgccat acttactgat
     8761 gaataaaagt actaatgatt aaacaaacta tttttaaatg gcaaattgaa ataatgcgaa
     8821 ttttaaactt ataaattaat ggctataaac tattttatat ctatctatgt tttaactact
     8881 taaattatta gacaaaacaa ttttcttaaa ttatcaatta aagtatttaa aaatattgat
     8941 ggttaatgta aaatatcaat ataaataagt tttttaaatt tatgtatgtt tatttgattc
     9001 aaacaaaata acttaagagg agaaagttta tgaaaaagac actgggatgt ttacttttaa
     9061 ttatgctttt agtcgtagca ggttgttctt ttggtgggaa tcataaatta tcatcaaaga
     9121 aatcagaaga atcaaaacaa gaaactgtaa aaaaagaatc ggaagaagag aaagatccag
     9181 atttagagaa atatgaagaa atagagaaga aaatgaaagg aattaaagat gcgccatctc
     9241 ttgataagtt ggatccatta atgacagaaa agtcttttac gaatagtaaa gggattcaag
     9301 gatggaaaga ttacaaagaa ttaatgggta aagtggaact tgcagattat agatttacta
     9361 aagattcaaa aggatcttca ataaaagatg ttgatgcatt ctttaaaggt aagaaaggta
     9421 taaaaaggaa agtgattgaa acacacgatg atgtaaaaca agttgattat tggtatgtag
     9481 atccagatgg aaagaaaatt ggcaattcaa acacacctgt tttttacgca gaaattatga
     9541 caaaatataa agatggaaag ttagtttatg catcagtcga accaggatct tacgtaatac
     9601 ataaagatga tgcaattaaa tatgacgatt attctaagtt aaaaaaatta agtcagctaa
     9661 ctaaacttga tcatccaaaa ccagttccat atagcgtagc tcaaatcaaa tctttcggag
     9721 tacctttaac aagcgtttca tttatgacac atggatcaaa ggatactaaa gatgaagtgt
     9781 tgccggcatt ggcctatttc actttttcac caaaaaatta tgaagacaag tctaatccag
     9841 atccaaaagt tttaaattta gtacatatgg atttcttaaa tgcatctagt gattttggta
     9901 acgcacattt tgttgtttta agtaaatata ttaaagagta tgaatcaaac tatgaaacag
     9961 cgtcagatga ttctttaaaa tagtatttac tgtgtgaaaa ataaatagtg tactacatta
    10021 aataatcgca ataataatcc cgataaacaa tcagcattac tgcttatcac atagagttcg
    10081 taataactat aactctatga ttcgcaaata ataaatgatt gtcatcggga tttattttta
    10141 tcaatttata aagtgacatt accttgttca tcagcaggtt tgaaaacagt aatcactgca
    10201 ctaataattg ctaaaatgtg tgggatacct gtccaacaga atattaagtg tagaatacct
    10261 tgcatattct tgccggcata aaatttatga ataccaaaac tacctaagaa caatgctaat
    10321 aaaatataaa taactttgtt tacttgcatt tctttccctc cagttgaatt gcttataatg
    10381 acattagctt ctctttttat tatacccact tttagttcaa acattctagt ttaagcattc
    10441 ccaatcatct aaatttcagt tattcaatcc ttacaataaa tttaggatta catttcagtt
    10501 gcattgtatt attttacgtg tgaaatatac gtaatgaatc acatgacaat cttcaaattg
    10561 aaaaatatac attctatgat gtaaggtcgc atttttaata tatttacgtt ataatagttt
    10621 gatgaatttt attgaggagt aagagttatg gcgattaaaa agttagtacc agcatcgcat
    10681 cctattttaa cgaaaaaagc gcaagcagtt aaaacatttg atgattcgtt aaaaagatta
    10741 ttacaagatt tagaagatac aatgtatgca caagaagctg ctggcttatg tgcacctcaa
    10801 attaatcagt cattgcaagt ggcaattatt gatatggaaa tggaaggatt attacaactt
    10861 gttaatccga aaattattag tcaatcaaat gaaacaataa cagacttaga aggttcaatt
    10921 acattgccag atgtttacgg cgaagtgaca agaagtaaaa tgatagttgt cgaaagttat
    10981 gacgtcaatg ggaacaaagt tgaactaact gcacatgaag atgtagcaag aatgattttg
    11041 catattatag atcaaatgaa cggtatccct tttacagaac gtgcggaccg tattttaaca
    11101 gataaagaag tggaggcata ttttataaat gactaaaata atatttatgg gtacaccaga
    11161 cttttcaaca actgttttag aaatgcttat tgcagaacat gatgtcattg cagtcgtaac
    11221 gcaaccagat cgacctgttg gacgtaaacg tgttatgaca ccaccaccag ttaaaaaagt
    11281 tgcaatgaaa tatgatttac ctgtatatca acctgaaaaa ttaagtggat cagaagaatt
    11341 agaacaattg cttcaattag atgtagattt aattgtaact gctgcttttg gacaattatt
    11401 acctgaatca ttgttggcat taccaaatct tggggcaatt aatgtacatg catcattgtt
    11461 accgaagtat agaggtggtg caccaattca tcaggcaatt atcgatggtg aacaagaaac
    11521 cggcataaca attatgtata tggttaaaaa attagatgcg ggtaatatta tttcgcaaca
    11581 agcaattaaa atagaagaaa atgataatgt cggtacgatg catgataaat taagtgtatt
    11641 aggggcagat ttattaaaag aaactttacc atctattata gagggcacaa atgaaagcgt
    11701 acctcaagat gatacgcaag caacatttgc ttccaatatt cgacgcgaag atgagcgaat
    11761 tagctggaat aaaccaggaa gacaagtgtt taatcaaatt cgtggattat caccatggcc
    11821 agttgcttat acaactatgg atgacactaa cttgaaaata tacgatgctg aactcgttga
    11881 gactaataag ataaacgagc ctggaaccat tatagaaacg actaaaaaag ccattattgt
    11941 tgctacaaat gataatgaag ctgttgcaat taaagatatg caattagctg ggaaaaagag
    12001 aatgttagct gccaattatt taagtggtgc gcaaaacaca ctagtaggga agaaacttat
    12061 atgatagaaa acgtgagaag tcttgctttt gacacgattc aagatatatt aaatgaaggt
    12121 gcgtatagta acttgcgtat caatgaagtg ttgtcagaaa atgaattaaa tgcaatggat
    12181 aaggctttat ttacagaaat tgtctacgga accgttaaaa gaaaatatac gttagatttt
    12241 tatttaaagc cttttgtgaa aacaaagatt aaggcatggg ttaggcaatt attatggatg
    12301 agtatttatc aatatgttta tttagataaa gttccaaatc atgccattat taatgaagca
    12361 gttgaaatag caaaagaacg cggtggctat cataatggta atgtcgtaaa tggtatttta
    12421 cgtacaatga tgcgtagtga cttacctgat tttaatgaaa ttgcagatcc taaaaaaaga
    12481 atggcaatcg agtatagtat gccgaagtgg attatagatc attgggcaac acattatggt
    12541 ctcgaagaaa ctgaaacaat tttacagtca tttttagaaa cgacatcaac aactgtgcgt
    12601 gccaacctga cgcgagcatc attagatgat attattgaaa agttgcaaga cgaaggttat
    12661 gacgttgaaa aagatcatga cttaccttat tgtctccata taggaggaca accaattatt
    12721 cattctcgtt catttaaaga tggattcgtt tcaattcaag ataaaagctc aatgtttgtt
    12781 gcacacatta tgaatgtaga ccgacatgat cacgtattag atgcatgtag tgcacctggc
    12841 ggtaaagctt gtcacattgc tgaagtttta atgccagaag ggcaagttga cgcttcagat
    12901 atacatgatc acaaaataga cttaattaat tttaatataa aaaaattacg attaacaaat
    12961 attaaagctt ttcaacatga tgcgacaaaa ccttatgata aaacatacga taagatactt
    13021 gttgatgcac catgtagcgg attaggtgta atgagacata agccggagat taagtatact
    13081 caaagcaaac aacatattga gtcactagtt gaattacagc ttgaaatatt ggaaaatgta
    13141 aaaaacaatg taaaaatagg tggcgaaatc atctattcaa catgtacaat tgagcaacta
    13201 gaaaatgaaa acgtgattta tacgtttttg aaaaataata aaaacttcga atttgaaccg
    13261 tttcaacatc cgataactgg agagttggtc aaaacgttac aaatcatgcc gcaagacttt
    13321 aattcagatg gattctttat cactaagata aaaagaaagg acaattagga atgataactg
    13381 ctgaaaagaa aaagaagaat aaatttcttc caaattttga caagcaatca atatattcat
    13441 tgcgatttga cgaaatgcaa aactggctcg ttgaacaagg gcaacaaaaa tttcgagcga
    13501 aacagatttt tgaatggtta tatcaaaaaa gagtagattc gattgatgaa atgacgaact
    13561 tatcgaaaga cttacgacag cttttaaaag ataactttac tgttacaact ttaacaactg
    13621 tagtaaaaca agaaagtaaa gacggtacaa ttaaattctt atttgaatta caagatggct
    13681 atacaattga aactgtttta atgagacatg attatggaaa ttcagtatgt gtaacgacac
    13741 aagtaggttg tcgcatcgga tgtacgtttt gtgcttctac acttggcggc ttaaaaagaa
    13801 accttgaagc tggcgaaatt gtttcacaag ttttaacagt tcaaaaagcc cttgatgcta
    13861 cagaagagcg cgtatctcaa attgtcataa tgggtatcgg tgaaccattt gaaaattatg
    13921 atgaaatgat ggacttttta agaatcgtca atgatgataa cagtttaaat attggtgcac
    13981 gtcacattac agtatcaaca tcaggtatca ttcctagaat atatgacttt gcggatgaag
    14041 atattcaaat taattttgct gtaagcttac acgccgcaaa agatgaagtg cgatcacgct
    14101 tgatgccaat taaccgtgca tataatgttg agaagttaat cgaagcaatt caatattatc
    14161 aagaaaaaac aactcgtcgt gttacttttg aatatggtct gtttggtggt gtgaatgacc
    14221 aactagaaca tgcaagagaa ttagcacatt taataaaagg cttaaactgc catgttaact
    14281 taattcctgt caaccatgtt ccagaaagaa attatgtgaa aacggctaaa aatgatatct
    14341 ttaaatttga aaaagaatta aagagactag gaattaatgc cacaatacgt cgtgaacaag
    14401 gttcggatat tgacgcagct tgtggtcaat taagagcaaa ggaacgacaa gtagaaacga
    14461 ggtaaagaca aatgctagag gcacaatttt ttactgatac tggacaacat agagataaga
    14521 atgaagatgc gggtggtatt ttttataatc aaactaatca acaactttta gttctgtgtg
    14581 atggtatggg tggccataaa gcaggagaag ttgcaagtaa atttgttaca gatgagttga
    14641 aatcccgttt tgaagcggaa aatcttatag aacaacatca agctgaaaat tggttgcgta
    14701 ataatataaa agatataaat tttcagttat atcactatgc acaagaaaat gcagaatata
    14761 aaggtatggg tacaacatgt gtttgtgcac ttgtttttga aaaatcagtt gtgatagcaa
    14821 atgtcggtga ttctagagcc tatgttatta atagtcgaca aattgaacaa attactagtg
    14881 atcactcatt tgttaatcat cttgttttaa cgggtcaaat tacgccggaa gaagcattta
    14941 cacatccaca acgtaatatt attacgaagg tgatgggcac agataaacgt gtgagtccag
    15001 atttgtttat taagcgatta aatttttatg attatttatt attaaattca gatggattaa
    15061 ctgattatgt taaagacaat gaaattaagc gtttgttagt aaaagaaggt acaatagaag
    15121 atcatggtga tcaattaatg caattggcat tagataacca ttcgaaagat aacgttactt
    15181 tcatactcgc ggctattgaa ggtgataaag tatgataggt aaaataataa atgaacgata
    15241 taaaattgta gataagcttg gcggcggtgg catgagtacc gtttatcttg ctgaagatac
    15301 gatacttaac attaaagttg caattaaggc gatttttata ccacctagag aaaaagaaga
    15361 aacattaaaa cgttttgaac gagaagtaca taactcatca cagctatcac atcaaaatat
    15421 agtaagtatg atcgatgttg atgaagaaga tgactgttac tacttagtaa tggaatatat
    15481 cgaaggtccg actttgtctg agtatattga aagtcatggg ccattaagtg ttgacacagc
    15541 gattaatttt acgaatcaaa tattggatgg cattaaacat gcgcatgata tgcgtattgt
    15601 acatagagat attaagccac aaaatatatt aattgacagc aataaaacgt tgaaaatatt
    15661 tgattttgga attgctaaag ctttaagtga gacgtcttta actcagacta atcatgtgtt
    15721 aggtactgtg cagtactttt cgccagaaca agcaaaaggt gaggcaacgg atgaatgtac
    15781 agatatttat tctataggta ttgtgttata tgaaatgctt gttggtgaac caccctttaa
    15841 tggagaaact gcagttagca ttgcgattaa acatattcag gattctgtgc caaatgtgac
    15901 aacagatgta cgtaaggata ttccgcaatc tttaagtaat gtcattttac gcgctacaga
    15961 aaaagacaaa gcgaatcgtt acaaaacaat tcaagaaatg aaagatgatt tgagtagtgt
    16021 tttacatgaa aatcgagcga atgaagatgt ctatgaactc gataaaatga aaacgatagc
    16081 ggtacctttg aaaaaagaag atctagcaaa gcatattagt gaacataagt cgaatcaacc
    16141 taaacgtgaa acgacgcaag tacctattgt aaatgggcct gctcatcatc agcaattcca
    16201 aaagccagaa ggtacggtgt acgaaccaaa acctaaaaag aaatcaacac gaaagattgt
    16261 gctcttatca ctaatctttt cgttgttaat gattgcactt gtttcttttg tggcaatggc
    16321 aatgtttggt aataaatacg aagagacacc tgatgtaatc gggaaatctg taaaagaagc
    16381 agagcaaata ttcaataaaa acaacctgaa attgggtaaa atttctagaa gttatagtga
    16441 taaatatcct gaaaatgaaa ttattaagac aactcctaat actggtgaac gtgttgaacg
    16501 tggtgacagt gttgatgttg ttatatcaaa gggccctgaa aaggttaaaa tgccaaatgt
    16561 cattggttta cctaaggagg aagccttgca gaaattaaaa tcgttaggtc ttaaagatgt
    16621 tacgattgaa aaagtatata ataatcaagc gccaaaagga tacattgcaa atcaaagtgt
    16681 aaccgcaaat actgaaatcg ctattcatga ttctaatatt aaactatatg aatctttagg
    16741 cattaagcaa gtttatgtag aagactttga acataaatcc tttagcaaag ctaaaaaagc
    16801 cttagaagaa aaagggttta aagttgaaag taaggaagag tatagtgacg atattgatga
    16861 gggtgatgtg atttctcaat ctcctaaagg aaaatcagta gatgaggggt caacgatttc
    16921 atttgttgtt tctaaaggta aaaaaagtga ctcatcagat gtcaaaacga caactgaatc
    16981 ggtagatgta ccatacactg gtaaaaatga taagtcacaa aaagttaaag tttatattaa
    17041 agataaagat aatgacggtt caactgaaaa aggtagtttc gatattacta gtgatcaacg
    17101 tatagacatt cctttaagaa ttgaaaaagg aaaaacagca agttatattg ttaaagttga
    17161 cggtaaaact gtagctgaaa aagaagtcag ctatgatgat gtataaatat aattgaagta
    17221 aatgtaccga ggtttctatt tggaagtctc ggtattttta tgttgagatt gcggtagttt
    17281 taaaatgctt catgtcatca tatacaggtg agcattgtct atcaattctg tatcactctc
    17341 acaatattta tatcatttga aatgatcatt tatatattaa ttttccagtc tttgatataa
    17401 taaatgcagt aaaattaatc gagaggtgcc attttgaaga caggtcgaat agtgaaatca
    17461 attagtgggg tatatcaagt agacgttaat ggcgaacgtt tcaatacaaa accacgagga
    17521 ttatttagaa agaaaaaatt ttcaccggta gttggtgata tagtggaatt tgaagttcaa
    17581 aacattaacg aaggttatat tcatcaagtg tttgagcggg aaaatgagtt gaaaagacca
    17641 cctgtaagta atatagatac actagtaatt gtaatgagtg ctgtcgagcc aaatttttca
    17701 acgcaattat tagatcgatt tttagttatt gcacattcgt atcagttaaa tgcgagaatt
    17761 ttggtgacta aaaaagataa aacaccaatt gaaaagcagt tcgaaataaa tgagttgttg
    17821 aaaatatatg aaaatattgg ctatgagact gaatttattg gaaatgatga tgatcgaaaa
    17881 aaaattgtag aagcttggcc agctggactt atagtactta gtggtcaatc aggtgtcggt
    17941 aagtccactt tcttaaatca ttatcgtcca gaacttaatc ttgagacaaa tgatatatca
    18001 aaatcattaa atcgaggaaa gcatactaca agacatgtcg aactattcga acgtcaaaac
    18061 ggttatattg cagacacacc tggattcagt gctttagatt ttgatcatat agataaagat
    18121 gaaataaaag attattttct tgaattaaat cgatatggtg aaacatgtaa gtttaggaat
    18181 tgtaatcata tcaaagaacc taattgtaat gttaagcatc aattagagat agggaatatt
    18241 gcgcaattta gatacgacca ttatttacaa ctatttaatg aaatttcaaa tagaaaggtt
    18301 agatattaaa tgacaaaact atatccatca ttattatctg ttgatttttt ggatttacaa
    18361 catgaattaa aacgacttga agaagcaggc gtcgacggag ttcattttga tgttatggat
    18421 ggtcaatttg tgcctaatat atctattggt ttaccaatat tagatgcagt aagaaaaggc
    18481 acaacattac ctatagacgt acatttgatg attgaaaatc cagaaaagta tattgcatca
    18541 tttgcagaac atggtgccga tatgatttca attcatgtcg aatcaacgcc tcatattcat
    18601 cgtgctattc aaatgattaa acatttagat aaaaaagctg gtgtagtaat taatcctggt
    18661 acaccaatat cacaaattga acctatttta gacattgttg attatgtact agtgatgaca
    18721 gttaacccag ggtttggtgg tcaatcattt attgatcaat gcgtagaaaa aatagcgggt
    18781 cttaatgcta ttaaaatgga acgtcaatta aactttgata ttgaagttga tggaggcgta
    18841 aataccgata cagcgaaagt ttgtgttgaa aatggtgcta caatgctagt aacaggttca
    18901 tttttcttta aacaagagga ttataaaaaa gtcacacaag cattgaaagg ttgagtgtat
    18961 atgcatataa atttattatg ttctgatcga cacttgccgc aagatatttg ggccaaaagt
    19021 aatgaaggta aatggggcgg cgttgataga ggtgctttga ttttattgaa gcatcaaatt
    19081 atcccttttt tctcagtggg agactttgat tcagtcagta aagaagaacg ccaacttcta
    19141 acagaacagt tacaaatcaa accagttcaa gctgaaaaag ctgatacgga tttagcttta
    19201 gcggttgata aagctgttgc acttggattt gatagtatta caatttatgg tgcaacaggc
    19261 ggacgattag atcacttttt tggggcaatt cagttattat tgaaaaaagc atattataaa
    19321 catgatgttc atatagaagt tatcgatcaa caaaataaaa ttgaattatt gcctaaaggt
    19381 caacatacag ttgaaaaaga taagagttat ccgtacattt catttatacc gatgactgat
    19441 gatgtagaac tttctctagc aggttttaaa tataatttag ctagacaaat gcttaatata
    19501 ggttctactt taactatttc aaatgaaatt gagtctttgc aagcgaaagt aactgtacat
    19561 gatgggttga ttttgcaaat tagaagtaca gatttaaatt aagtatattc aaatttgtga
    19621 ggatttttaa taaagtgcat gagttattta ataaagtgac gtttatagat agaatttata
    19681 acataataat agatgaatca cgctgtaatt aatgaagatt atggcgtgat ttattattga
    19741 aaggaaaatt gtaggtccta gtacattgat ttatgtgcca aaatcgatat tgaacccata
    19801 agtccaattt gacagagaac cattattaaa cgtattaacc aaattatatt gatagttcga
    19861 atttcactat gaaccattat gtattgtttg gtaacaaaaa aagaactaag aggttgagct
    19921 cttagttctt aagtaaatgt gtcctccaca taaggtcaca ttttttatgt gcaatatatt
    19981 tattaaactc tagttacttt accagatttt aaagcacgtg cagaaaccca aactttttta
    20041 ggtttaccgt caactaggat tctaactttt tgaaggttag cgttccatct acgtttagta
    20101 gagtttaaag cgtgtgaacg tctgttacca gtcgaagctt tacgacctgt tacgaaacat
    20161 tgtttaccca tatgagtacc tcctttaata aatataaata cacataacta cttatatact
    20221 taattaagat agcatagttt catttgaaaa acaatgaata attttcacat aaaagtcaaa
    20281 aatactgagt tttgtgatat aattgtagac tgtgaagtta tgtagtatga tattttagag
    20341 aaataagtga atgaaacatt taaaattttt atttaatgat actacatcta ttaaaagaaa
    20401 acgcctatag ataatcgtca tgagtgtaaa ttcagtttac tcaaaatata ataggtgatt
    20461 taagcgtaag tcattctgat aagttgtacc attgatattt tataaaaata atcaatgaaa
    20521 cttaaagcgt ttatgactac actaagtggt aaaattttaa aagtgattag ggaaaacgaa
    20581 taataaactt tgaaatttag gagggacaaa gatatgacat tagagatttc aaatgattac
    20641 ggtaaaattg atatttcaaa cgaagtgatt gcttcggttg taggtggaaa ggccgttgaa
    20701 tgttatggta ttgtaggtat ggcatctaga caacaagtta gagatggtat tgcggaaata
    20761 ctaggacatg aaaattatgc taaaggcatc aaagtaactg aaaataatgg cgtagtggat
    20821 atagatatgt acattattgt aagttacggt gtgaaaatat ctgaagttgc caataatgta
    20881 caatcaacag tgaaatatac tttggaaaaa tcacttaatg tatcagtaaa ttcaatcaat
    20941 atatatgtac aaggtgtacg tgtgaataat acaggcaaga aagcttagga ggacaacttg
    21001 aaatgattag caaaattaat ggtaaattat ttgccgatat gattatacaa ggggcacaaa
    21061 atttatctaa caatgcagat ttggtagatt ctttgaatgt gtatccagtg ccagatggtg
    21121 atacaggaac aaatatgaat cttactatga cttcaggtcg cgaagaagta gagaataatt
    21181 tgtcgaaaaa tatcggcgaa ttaggtaaaa cattctcgaa aggtttacta atgggtgcaa
    21241 gaggtaactc tggtgtcatc ttgtcacaat tattcagagg attttgtaaa aatattgaaa
    21301 gtgaatctga aattaattca aaattgttag ctgaaagttt tcaagctggt gttgaaacgg
    21361 catataaagc tgttatgaaa ccagttgaag gtacaatact tacagttgca aaagatgctg
    21421 cgcaagctgc aatagaaaaa gcaaataata ctgaagattg tatagaatta atggagtaca
    21481 ttattgtaaa agccaatgaa tcacttgaaa acacaccaaa cttattagct gtacttaaag
    21541 aagttggtgt tgttgatagt ggcggtaaag gtttgttatg cgtttacgaa ggattcttaa
    21601 aagcgcttaa aggtgaaaaa gttgaagcca aagttgcaaa gatagataaa gatgaatttg
    21661 tacatgatga acatgatttc catggtgtaa ttaatactga agatattatt tatggctatt
    21721 gtactgaaat gatggttcgt tttggaaaga ataaaaaagc ctttgatgaa caagaattca
    21781 ggcaagatat gagtcaattt ggtgattctt tattagtcat taatgatgaa gaaattgtga
    21841 aagttcacgt gcataccgaa tacccaggta aagtgtttaa ttatggtcaa caatatggtg
    21901 aattaattaa acttaaggtt gaaaatatga gagaacagca ccgtgaagtg attcgaaaag
    21961 aacagcacac agctaaaccg aaaatggaaa cggttgaaac agcaattatt actatttcta
    22021 tgggtgaagg tatttcagag atatttaaat caatgggtgc cacacatatc attagtggtg
    22081 gacaaacgat gaatccttct acagaagata tcgttaaagt cattgaacaa tcaaaatgta
    22141 aacgtgcaat tattttaccg aataataaaa atatcttaat ggcaagtgaa caagcagcga
    22201 gtattgttga tgcagaagct gttgttattc caacgaaatc tattcctcaa ggtataagcg
    22261 cactattcca atatgatgtg gacgcaacac ttgaagaaaa taaagcgcaa atggctgatt
    22321 cagtaaataa cgttaaatct ggttcattaa cgtacgctgt tcgtgatacg aaaattgatg
    22381 gcgttgagat taaaaaagac gcgtttatgg gcttgattga agataagatt gtaagcagcc
    22441 aaagtgatca attaacaacg gttactgagt tgttaaatga gatgttagca gaagatagtg
    22501 aaatattgac tgtgattatt ggtcaagatg cagagcaagc agttacagat aacatgataa
    22561 actggatcga agagcaatat ccagatgtag aagtggaagt tcatgaaggt ggacaaccaa
    22621 tttatcaata tttcttttca gtagaataaa aatttaaaat aaaaaactac caatgataaa
    22681 tcatcagttg gtagtttttt attttgctat tttagtgata ttgcgggtta aaagtatcgt
    22741 tctcgagttg ctaacaatgt catgttcaac ttagtcatga taaaataaat aacatactaa
    22801 atgatacgta aaatcaaata aaacataggt gatttatttt ggctaaagta aacttaatag
    22861 aaagtccata ttctctttta caattaaaag gtataggtcc taagaaaata gaagtattgc
    22921 aacaactaaa tattcataca gtggaagatc ttgttcttta tttgccaact agatatgaag
    22981 ataatacagt gattgatttg aatcaagcag aagatcaatc taacgttacg atagaaggac
    23041 aagtatatac agctccagta gttgcatttt ttggaagaaa taaatcaaaa ttaaccgttc
    23101 atttaatggt aaataatatt gctgtcaaat gtattttttt caatcaaccg tatttaaaaa
    23161 agaaaatcga attaaatcaa actataactg ttaaaggtaa gtggaatagg gttaaacagg
    23221 aaattactgg taatagggtt ttctttaatt cacaagggac acaaactcaa gaaaacgcag
    23281 atgttcaatt agaaccagtc tatcgtatta aggaaggtat taaacaaaag caaatacgag
    23341 accaaattag acaagcgtta aatgatgtga caattcatga atggttaact gatgaactaa
    23401 gagaaaaata taaattagag accttggact ttactttgaa cacattacat catcctaaaa
    23461 gtaaagagga tttattacgt gctcgtagaa cctatgcatt tactgaactg tttttattcg
    23521 aattacgtat gcaatggcta aatagattag aaaagtcatc tgacgaagca attgaaattg
    23581 attatgacat agaccaagtt aaatcattta ttgatcgttt accttttgaa ctaactgaag
    23641 cacagaaatc cagtgttaat gaaattttta gagatttaaa agcaccaata cgtatgcatc
    23701 gattacttca aggtgatgta ggttcaggaa aaacagtagt tgctgcaatt tgtatgtatg
    23761 cgttaaaaac tgctggttat caatcagcat tgatggtacc aactgaaatt ttagcagagc
    23821 aacatgctga aagtttaatg gctttatttg gagattctat gaacgttgca ttgttaactg
    23881 ggtcagtaaa aggtaagaaa cgaaagatac ttttagaaca acttgaaaat ggtacgattg
    23941 attgtttaat tggaacccat gctttgattc aagatgatgt gattttccat aatgttggtt
    24001 tagtaattac agatgaacaa catcgatttg gtgtgaatca acgccagctt ttaagagaaa
    24061 aaggtgcaat gacgaatgtg ttatttatga cagcaacgcc gataccaaga acactagcaa
    24121 tatcagtttt tggtgagatg gatgtgtctt caattaaaca attaccaaaa ggtcgtaaac
    24181 ctatcattac tacttgggca aagcatgagc aatacgataa agttttgatg caaatgacct
    24241 cagagttgaa aaaaggtcgt caagcatatg tcatttgccc gctaatagaa agttctgagc
    24301 atctcgaaga tgttcaaaat gttgtcgcat tgtacgagtc tttacaacag tattatggtg
    24361 tttcccgtgt agggttattg catggtaagt tatctgccga tgaaaaagat gaggtcatgc
    24421 aaaagtttag taatcatgag ataaatgttt tagtttctac tactgttgtt gaagtaggtg
    24481 ttaatgtacc gaatgcaact tttatgatga tttatgatgc ggatcgcttt ggattatcaa
    24541 ctttacatca gttacgcggt cgtgtaggta gaagtgacca gcaaagttac tgtgttttaa
    24601 ttgcatcccc taaaacagaa acaggaattg aaagaatgac aattatgaca caaacaacgg
    24661 atggatttga attgagtgaa cgagacttag aaatgcgtgg tcctggagat ttctttggtg
    24721 ttaaacaaag tggattgcca gatttcttag ttgccaattt agttgaagat tatcgtatgt
    24781 tagaagttgc tcgtgatgaa gcagctgaac ttattcaatc tggcgtattc tttgaaaata
    24841 cgtatcaaca tttacgtcat tttgttgaag aaaatttatt acatcgtagt tttgactaat
    24901 tgccatgctg atttgtcaat ttgagtgcaa cacttcgtta attgagtgat atgacacttg
    24961 aactatttaa atgtaaagtg gtattttaac aatttataaa ttttcgacta aataatagct
    25021 aaatattaca gttatttgtt gagtcggtta aatagaaagt gttatgatat gtgaggaatg
    25081 tttaagacta ggtactaaaa aatgaggggt gagacgttga aactaaagaa agataaacgt
    25141 agagaagcaa tcagacaaca aattgatagc aatcccttca tcacagacca tgaactaagc
    25201 gacttatttc aagtgagtat acaaacaatt cgtttagatc gcacttattt aaacatacca
    25261 gaattaagga agcgtattaa attagttgct gaaaagaatt atgaccaaat aagttctatt
    25321 gaagaacaag aatttattgg tgatttgatt caagtcaatc caaatgttaa agcgcaatca
    25381 attttagata ttacatcgga ttctgttttt cataaaactg gaattgcgcg tggtcatgtg
    25441 ctgtttgctc aggcaaattc gttatgtgtt gcgctaatta agcaaccaac agttttaact
    25501 catgagagta gcattcaatt tattgaaaaa gtaaaattaa atgatacggt aagagcagaa
    25561 gcacgagttg taaatcaaac tgcaaaacat tattacgtcg aagtaaagtc atatgttaaa
    25621 catacattag ttttcaaagg aaattttaaa atgttttatg ataagcgagg ataaaattat
    25681 ggttaaatta gcaattgata tgatgggtgg cgacaatgcg cctgatatcg tattagaagc
    25741 cgtacaaaag gctgttgaag actttaaaga tctagaaatt atacttttcg gtgacgaaaa
    25801 aaagtataat ctgaaccatg aacgaatcga atttagacat tgttctgaaa agattgaaat
    25861 ggaagatgag cctgttagag cgattaaacg taaaaaagat agctcaatgg taaaaatggc
    25921 tgaagctgtg aaatctggtg aagcagatgg atgtgtgtca gcaggtaata ctggtgcttt
    25981 aatgtcagct ggtttattca ttgttggacg tattaaaggt gtagctagac cggctttagt
    26041 agtaacattg ccaacgattg atggaaaagg ttttgtcttt ttagacgttg gtgcaaatgc
    26101 tgatgctaaa cctgaacact tattacagta tgcgcaacta ggggatattt atgctcaaaa
    26161 aattagaggt attgataatc cgaaaatctc attattaaat ataggaaccg agccagctaa
    26221 aggtaatagt ttaacgaaaa aatcatatga gttattaaat catgatcatt cattgaattt
    26281 tgttgggaat attgaagcga agacattaat ggatggcgat acagatgttg tagttaccga
    26341 tggctatact gggaacatgg tccttaaaaa tttagaaggt actgcaaaat caatcggtaa
    26401 aatgttaaaa gatacgatta tgagtagtac taaaaataaa ttagcaggtg caatattgaa
    26461 gaaagattta gctgaattcg ctaaaaagat ggattactca gaatacggtg gttccgtatt
    26521 attaggattg gaaggtactg tagttaaagc acacggtagt tcaaatgcta aagcttttta
    26581 ttctgcaatt agacaagcga aaatcgcagg agaacaaaat attgtacaaa caatgaaaga
    26641 gactgtaggt gaatcaaatg agtaaaacag caattatttt tccgggacaa ggtgcccaaa
    26701 aagttggtat ggcgcaagat ttgtttaaca acaatgatca agcaactgaa attttaactt
    26761 cagcagcgaa cacattagac tttgatattt tagagacaat gtttactgat gaagaaggta
    26821 aattgggtga aactgaaaac acacaaccag ctttattgac gcatagttcg gcattattag
    26881 cagcgctaaa aaatttgaat cctgatttta ctatggggca tagtttaggt gaatattcaa
    26941 gtttagttgc agctgacgta ttatcatttg aagatgcagt taaaattgtt agaaaacgtg
    27001 gtcaattaat ggcgcaagca tttcctactg gtgtaggaag catggctgca gtattgggat
    27061 tagattttga taaagtcgat gaaatttgta agtcattatc atctgatgac aaaataattg
    27121 aaccagcaaa cattaattgc ccaggtcaaa ttgttgtttc aggtcacaaa gctttaattg
    27181 atgagctagt agaaaaaggt aaatcattag gtgcaaaacg tgtcatgcct ttagcagtat
    27241 ctggaccatt ccattcatcg ctaatgaaag tgattgaaga agatttttca agttacatta
    27301 atcaatttga atggcgtgat gctaagtttc ctgtagttca aaatgtaaat gcgcaaggtg
    27361 aaactgacaa agaagtaatt aaatctaata tggtcaagca attatattca ccagtacaat
    27421 tcattaactc aacagaatgg ctaatagacc aaggtgttga tcattttatt gaaattggtc
    27481 ctggaaaagt tttatctggc ttaattaaaa aaataaatag agatgttaag ttaacatcaa
    27541 ttcaaacttt agaagatgtg aaaggatgga atgaaaatga ctaagagtgc tttagtaaca
    27601 ggtgcatcaa gaggaattgg acgtagtatt gcgttacaat tagcagaaga aggatataat
    27661 gtagcagtaa actatgcagg cagcaaagag aaagctgaag cagtagtcga agaaatcaaa
    27721 gctaaaggtg ttgacagttt tgcgattcaa gcaaatgttg ccgatgctga tgaagttaaa
    27781 gcaatgatta aagaagtagt tagccaattt ggttctttag atgttttagt aaataatgca
    27841 ggtattactc gcgataattt attaatgcgt atgaaagaac aagagtggga tgatgttatt
    27901 gacacaaact taaaaggtgt atttaactgt atccaaaaag caacaccaca aatgttaaga
    27961 caacgtagtg gtgctatcat caatttatca agtgttgttg gagcagtagg taatccggga
    28021 caagcaaact atgttgcaac aaaagcaggt gttattggtt taactaaatc tgcggcgcgt
    28081 gaattagcat ctcgtggtat cactgtaaat gcagttgcac ctggttttat tgtttctgat
    28141 atgacagatg ctttaagtga tgagcttaaa gaacaaatgt tgactcaaat tccgttagca
    28201 cgttttggtc aagacacaga tattgctaat acagtagcgt tcttagcatc agacaaagca
    28261 aaatatatta caggtcaaac aatccatgta aatggtggaa tgtacatgta atatatttga
    28321 gctaaagctc attgacgcag tggttgactg gtcatccaat ggagaattgt ctgacctagt
    28381 caactttgcg ggggaaattc taagcaacct agataaggtt ccagaatttc tccctaagaa
    28441 acactaatca ataaattgat aagtgtttct aaaatttcta cttgtttttt agaatttaaa
    28501 atgggaaaat atagtagtct atgtataggc atttttaaag gaggtgaatc gacgtggaaa
    28561 atttcgataa agtaaaagat atcatcgttg accgtttagg tgtagacgct gataaagtaa
    28621 ctgaagatgc atctttcaaa gatgatttag gcgctgactc acttgatatc gctgaattag
    28681 taatggaatt agaagacgag tttggtactg aaattcctga tgaagaagct gaaaaaatca
    28741 acactgttgg tgatgctgtt aaatttatta acagtcttga aaaataataa atcttacatc
    28801 tgggtcgtca gtattgtcga ctcagttttt ttctttaatt atcaatagtt ttaacgtaaa
    28861 attaaagatg attcaagagc aacacataaa ggagataaaa taatgtctaa acaaaagaaa
    28921 agtgagatag ttaatcgttt tagaaagcgc tttgatacta aaatgacaga gttaggcttt
    28981 acttatcaaa atattgattt ataccaacaa gcattttcgc attcgagttt tattaatgat
    29041 tttaatatga atcgtttaga ccataatgag cgtttagagt ttttgggtga tgcggtatta
    29101 gaattgacgg tttcacgata tttatttgat aaacatccca acttgccaga agggaattta
    29161 acaaaaatgc gtgccactat tgtatgtgag ccctcacttg taatatttgc gaataaaatt
    29221 ggattgaacg aaatgatttt acttggtaaa ggtgaagaga aaacaggggg acgtacaaga
    29281 ccatcattaa tatcagatgc attcgaagca tttattgggg cattgtattt ggatcaagga
    29341 ctagatatag tttggaaatt tgctgagaaa gtcattttcc cacatgtaga acaaaatgag
    29401 ttattaggcg tggtagattt taaaacacaa ttccaagaat atgtgcacca gcaaaataaa
    29461 ggtgatgtaa cctataattt aataaaagaa gagggaccgg cacatcatcg tctattcact
    29521 tcagaagtta ttctgcaagg ggaagcaata gctgaaggta aagggaaaac gaaaaaagaa
    29581 tcagaacaac gtgctgctga aagtgcctat aagcaattaa aacaaattaa atagaaattt
    29641 atatgatacg acatataggt gttcgattta ggatttaagg aagaattttt aatattttat
    29701 ggaacaactt cgaaatttaa tatacaaata ctaaaatatc gtatacatgt gttctttaaa
    29761 attgtgataa ggagtttagg atggtttatt taaaatcaat agatgccatt ggatttaagt
    29821 cttttgcaga tcaaaccaat gttcaattcg ataaaggtgt aactgcaatt gttggtccaa
    29881 atggaagcgg taaaagtaat attacagatg ctattaaatg ggtgttgggt gaacaatcgg
    29941 ctaaatcatt acgtggctca aaaatggaag atattatctt ctcaggtgca gaacatcgca
    30001 aagctcaaaa ttatgctgaa gtacagttaa gattagataa tcattctaaa aagctcagtg
    30061 ttgatgaaaa cgaagttatt gtaacaagaa gattgtatcg aagtggtgaa agtgagtact
    30121 acataaataa tgaccgtgca agattaaaag atattgccga tttattttta gattctggat
    30181 tgggaaaaga agcgtatagc attatctcgc aaggtagagt tgatgaaata ctaaatgcta
    30241 aaccaattga tagacgtcaa attattgaag aatcggctgg tgtacttaaa tataaaaaac
    30301 gtaaggctga atcattaaat aaacttgacc aaacagaaga taatttaacg agagtagaag
    30361 acattttata tgatttggaa ggtcgcgtag aacctctaaa agaggaggca gctatagcta
    30421 aagaatataa gacactttca catcaaatga aacatagtga cattgtagtt acagtgcacg
    30481 atattgatca atatacaaat gacaatagac aattagatca acgtttaaat gatttacaag
    30541 gccaacaagc aaataaagaa gctgacaagc aacgtttaag ccaacaaatt caacaatata
    30601 aaggtaaacg tcatcaactt gataatgatg ttgaatcgct taattatcaa ttagtaaaag
    30661 ctacggaagc ctttgaaaaa tatacgggac aattaaatgt tttggaagaa cgtaagaaaa
    30721 atcaatctga aacaaatgca cgatatgaag aagaacaaga aaatttaatg gagcttttag
    30781 aaaatatatc aaatgagatt tctgaagctc aagatactta taagtctctg aaaagtaaac
    30841 aaaaagaact caatgctgtc attcgtgaac ttgaagaaca actatatgtt tcagacgaag
    30901 cacatgatga aaaattggaa gaaattaaaa acgaatacta tacattaatg tcagagcaat
    30961 cagatgttaa caatgatatt cgttttttaa agcatactat agaagagaat gaggctaaaa
    31021 aatcaagact agattctcga ttagttgaag tttttgagca attgaaagat attcagggtc
    31081 aaataaaaac gacaaaaaaa gaatatcaac agaccaacaa agaactttct gctgtagata
    31141 aagaaattaa aaatatagaa aaagacctca ctgatacaaa aaaagcacaa aatgaatacg
    31201 aagagaaatt gtatcaagca tatcgatata ccgaaaaaat gaaaacacgt attgatagtt
    31261 tggcaacgca agaggaagaa tatacttatt ttttcaatgg cgtcaaacat attttgaaag
    31321 ctaaaaataa agaattaaag ggtatttatg gtgcagttgc ggaaattatt gatgtgccat
    31381 ctaaattaac tcaggcaatt gaaacagcat taggtgcttc attacaacat gtcattgtag
    31441 attcagaaaa agatggacgc caggctattc aatttttaaa agaacgtaat ttaggtcgtg
    31501 cgacgttttt accattaaat gttatacaga gtagagtggt agcgactgat attaaatcta
    31561 ttgctaaaga ggcaaacgga tttattagta tcgcttcgga agcagttaaa gtagcaccag
    31621 aatatcaaaa tattatcggg aatttattag gtaatacgat tatcgttgat catttaaagc
    31681 atgcaaatga attggcacgt gcgattaaat atcgaactcg tattgttact ttggaaggtg
    31741 atattgtaaa tcctggtggt tctatgactg gtggtggcgc tcgtaagtca aaaagtattc
    31801 tgtctcaaaa agacgagttg acaacaatga gacaccaatt agaagattac ttgcgtcaaa
    31861 cagaatcatt tgaacaacaa tttaaagagt tgaagataaa aagtgatcaa ttaagtgaac
    31921 tgtattttga aaaaagtcaa aagcataata cacttaaaga gcaagtgcat cattttgaaa
    31981 tggagctcga tagattaact acacaagaaa cacaaataaa aaatgatcat gaagaattcg
    32041 aatttgaaaa aaatgatggt tatacgagtg acaaaagtcg acaaactttg agtgaaaaag
    32101 aaacttatct agaaagtatt aaagcatctt taaaacgact agaagatgaa attgaacgct
    32161 acacaaaact ttctaaagaa ggtaaggaaa gcgttactaa aacacaacaa accttacatc
    32221 agaaacaatc tgatcttgct gtggttaaag agcgtattaa aacacaacaa cagacaatag
    32281 atcgattaaa taatcaaaat caacaaacta aacatcaatt aaaagatgtt aaagaaaaaa
    32341 ttgcattctt taattcggat gaagtgatgg gcgaacaagc ttttcaaaat attaaagatc
    32401 aaattaatgg tcaacaagaa acgagaacac gcttatcaga tgaattagat aaattgaaac
    32461 aacaacgtat tgagttgaat gaacaaatcg atgcgcaaga agctaaacta caagtttgtc
    32521 accaagatat tttagctatc gaaaatcact accaagatat taaagctgaa caatcaaagc
    32581 tagatgtatt aattcatcat gcgatagatc atttaaatga tgaatatcaa ttgactgttg
    32641 aacgtgcgaa atctgaatat acgagtgatg aatcgattga cgcattacgt aaaaaagtta
    32701 agttaatgaa gatgtcgatt gatgaactag gtcctgtaaa cttaaatgca attgaacaat
    32761 ttgaagagtt aaatgaacgt tatacatttt taagtgaaca acgtacagat cttcgtaaag
    32821 ctaaagaaac attagagcaa attataagtg aaatggatca agaggttact gaaagattta
    32881 aagaaacttt ccatgctatt caaggacatt ttacagctgt gttcaaacaa ttgtttggtg
    32941 gaggcgatgc agaattgcaa ttaactgaag ccgattattt aacagctggt attgatattg
    33001 tggtacaacc accgggtaaa aagttgcaac atttatcgtt actgagtggt ggtgagcgtg
    33061 cattaactgc tattgcttta ctatttgcaa ttttaaaagt aagatctgca ccttttgtta
    33121 tattagatga ggttgaagct gcactagatg aagcaaatgt tattagatac gcaaaatatt
    33181 taaatgagtt atcagacgaa acacaattca ttgttattac acaccgtaaa ggaacaatgg
    33241 aatttgcaga taggttatac ggtgtaacaa tgcaagaatc aggtgttact aaacttgtga
    33301 gtgtgaattt aaatacaata gatgatgtgt tgaaggagga gcaataatga gcttttttaa
    33361 acgcttaaaa gataagtttg caacaaataa agaaaatgaa gaagttaaat ccttaacaga
    33421 agaacaaggt caagacaaat tagaagatac acattctgaa ggttcaacgc aggacgcaaa
    33481 tgatttagca gaaaatgctg aagtgaaaaa gaagccacgc aagttgagtg aagcggattt
    33541 tgatgacgat ggcttaatat caattgaaga ttttgaagaa attgaagctc aaaaaatggg
    33601 tgctaaattt aaagcaggac tcgaaaaatc tcgtcaaaat ttccaagaac aattaaataa
    33661 tttgatagcg agatatcgta aagtagatga agactttttt gaagctttag aagaaatgtt
    33721 aatcactgca gacgtcggtt ttaatacagt gatgacgtta actgaagaat tacgtatgga
    33781 agcacaacga cgtaatattc aagatactga agatttgcgt gaagtcattg ttgaaaaaat
    33841 cgtagagatt taccatcaag aagatgataa ttcagaagct atgaacttag aagatggtcg
    33901 tttaaatgtc attttaatgg ttggtgtgaa tggtgttggt aaaacaacaa caattggaaa
    33961 attagcttac cgatataaaa tggaaggtaa aaaagtaatg ttagctgcgg gcgatacttt
    34021 tagagcgggt gctattgatc aattgaaagt ttggggcgaa cgtgttggtg tagacgtaat
    34081 tagccaaagt gaaggttctg atccagctgc tgttatgtat gatgcgatta atgccgctaa
    34141 aaacaaaggt gttgatattt taatctgtga taccgctgga cgtttacaaa ataaaacaaa
    34201 tctaatgcaa gaattagaaa aagttaagcg tgtaattaat cgagcagtgc cagatgcgcc
    34261 tcatgaagca ttactatgtt tagatgctac aactggtcag aatgcgttgt cacaagctag
    34321 aaactttaaa gaagtaacaa atgttacagg tattgtatta acgaaattag atggtacagc
    34381 caaaggtggt atcgtattag ccattcgtaa tgaattgcac atcccagtta aatatgtagg
    34441 tttaggtgag caattagatg acttacaacc atttaaccct gaaagttatg tctacggctt
    34501 attcgctgat atgattgaac aaaatgaaga aataacaaca gttgaaaatg atcaaattgt
    34561 aacagaagaa aaggacgata atcatgggtc aaaatgattt agttaaaacg ttacgaatga
    34621 attatttgtt tgatttttat caatccttat tgacgaataa acaacgtaat tatttggaat
    34681 tattttatct tgaagattat tctttaagtg aaatcgcaga tacttttaat gtgagtagac
    34741 aagcagttta tgataatata agaagaactg gcgatttagt tgaagattat gaaaagaaat
    34801 tggaattata ccagaaattt gagcaacgcc gagaaatata tgatgaaatg aaacaacatt
    34861 taagtaatcc agaacaaata caacgttata ttcaacaatt agaagactta gaatagatat
    34921 aaattgaggg aggacatcga tatggcattt gaagggttat cagaacgctt gcaagcgacg
    34981 atgcaaaaaa tgcgtggtaa gggtaaactt actgaagctg atataaagat aatgatgcgt
    35041 gaagtaagat tagcgttact tgaggctgac gtaaacttta aagtggtaaa agaatttatt
    35101 aaaacagtat cagaacgcgc attaggttcc gatgtaatgc aatcattaac accagggcaa
    35161 caagttatta aaatagttca agatgaatta acgcagttga tgggtggaga aaatacgtcg
    35221 attaatatgt caaataaacc acctactgtt gttatgatgg ttggtttaca aggtgctggt
    35281 aaaacaacaa ctgcaggtaa attagcatta ttgatgcgta aaaaatacaa caaaaaacct
    35341 atgttagttg cagcagatat ttatcgtcca gcagcgataa atcaattaca aacagtaggg
    35401 aaacaaattg atattcctgt atacagtgaa ggagatcaag taaagccaca acaaattgta
    35461 actaatgcat taaaacatgc taaagaagaa catttagact ttgtaatcat tgatacagca
    35521 ggtcgattac acatcgatga agcattgatg aacgaattaa aagaagtaaa agacattgct
    35581 aaaccaaacg aaattatgtt agttgtcgat tcaatgacgg gtcaagatgc tgtcaatgtt
    35641 gcagaatctt ttgacgatca acttgatgtc acaggtgtta ccttaactaa attagatggt
    35701 gatacacgtg gtggtgcagc tttatctatt cgttcggtga cacaaaaacc aattaaattt
    35761 gttggtatga gtgaaaagtt agatggttta gagctattcc atcctgaacg tatggcatca
    35821 cgtattttag gtatgggtga tgtgttaagt ttaattgaaa aagcgcaaca agatgtggat
    35881 caagaaaaag caaaagattt agagaaaaag atgcgtgagt catcgtttac tttagatgat
    35941 tttttagaac aacttgatca ggtgaaaaat ctaggaccac tggatgatat tatgaaaatg
    36001 attccaggta tgaataaaat gaaagggcta gataagctta atatgagtga aaagcaaatt
    36061 gatcatatta aagcgattat ccagtcaatg acgccggctg aaagaaacaa tccagacaca
    36121 ttgaatgtat cacgtaaaaa gcgtattgct aaagggtctg gtcgttcatt acaagaagtc
    36181 aatcgtttga tgaaacaatt taacgatatg aagaaaatga tgaaacagtt cactggtggc
    36241 ggtaaaggta aaaaaggtaa acgcaatcaa atgcaaaata tgttaaaagg tatgaattta
    36301 ccgttttaat gtttaatata acacgctgat ttttgttgca atgagatgtg catcccgaaa
    36361 gttatactaa agaatataac tttatggggt gcatattttt agatacacaa taataattaa
    36421 aaactgaaat tgctgatttg ctagaacatg aggttattgt tgctaatatt aataagttat
    36481 attgggggaa cgtgtgcggt agaagtggta ccattcatgt ccttacgaga taaattaact
    36541 taacacgtag gtggtgaaat ttactatgaa tttttataac ctattaacag tctaatttag
    36601 taatcacata gttattattg taagaaaaag gttcaataat tttcctggta aagaaaaaac
    36661 tctttacaaa cattcataca cctgttaata ttatttcttg tagaaaataa aaattaaaac
    36721 atgacttaaa ggagatttta taaatggcag ttaaaattcg tttaacacgt ttaggttcaa
    36781 aaagaaatcc attctatcgt atcgtagtag cagatgctcg ttctccacgt gacggacgta
    36841 tcatcgaaca aatcggtact tataacccaa cgagcgctaa tgctccagaa attaaagttg
    36901 acgaagcgtt agctttaaaa tggttaaatg atggtgcgaa accaactgat acagttcaca
    36961 atatcttatc aaaagaaggt attatgaaaa aatttgacga acaaaagaaa gctaagtaat
    37021 ttagcgtaaa attgttctaa caataagaat aactcgttta cactgacagt tattactcaa
    37081 tgatacgttg ggaatatcac atgttagtaa tatagaacgt attgggtacc ataatggtgc
    37141 cctttttctt tgaattattt tcaattaaaa tagaagtgtt caaagcatag agttggaggt
    37201 aatagaatga gagttgaagt tggtcaaatt gttaacacac atggtattaa aggtgaaatt
    37261 aaagtaaaat ccaattcaga ctttacagac gttcgttttc aacccggtca agtgctgaca
    37321 gttgtgcata acaataacga ccttgaatat actgttaagt cacatagagt gcataaaggg
    37381 cttcatatgc ttacatttga aggtattaat aatattaatg atattgagca tttaaaaggg
    37441 agttctattt atcaagagcg tgatcatgaa gatatcgtac ttgaggaaaa tgaattttat
    37501 tattcagata ttataggatg tacagttttt gatgatcaag aaacaccaat aggtcgtgta
    37561 attaatatat ttgaaacagg tgcgaatgat gtgtgggtga ttaaaggatc taaagaatat
    37621 ttgattcctt atattgctga tgttgtaaaa gaagtggatg ttgaaaataa aaaaattatc
    37681 atcacgccaa tggaaggatt gttggattaa tgaaaattga ttatttaact ttatttcctg
    37741 aaatgtttga tggtgtttta aatcattcaa ttatgaaacg tgcccaagaa aacaataaat
    37801 tacaaatcaa tacggttaat tttagagatt atgcaattaa caagcacaac caagtagatg
    37861 attatccgta tggtggcgga caaggtatgg tgttaaagcc tgaacctgtt tttaatgcga
    37921 tggaagactt agatgtcaca gaacaaacac gcgttatttt aatgtgtcca caaggcgagc
    37981 cattttcaca tcagaaagct gttgaattaa gcaaggccga ccacatcgtt ttcatatgcg
    38041 gacattatga aggttacgat gaacgtatcc gaacacatct tgtcacagat gaaatatcaa
    38101 tgggtgacta tgttttaact ggtggagaat tgccagcgat gaccatgact gatgctattg
    38161 ttagactgat tccaggtgtt ttaggtaatg aacagtcaca tcaagacgat tcattttcag
    38221 atgggttatt agagtttccg caatatacac gtccgcgtga atttaagggt ctaacagttc
    38281 cagatgtttt attgtctgga aatcatgcca atattgatgc atggagacat gagcaaaagt
    38341 tgatccgcac atataataaa agacctgact taattgaaaa atatccatta actaatgcag
    38401 ataagcaaat attagaaaga tataaaatag gattgaaaaa aggttagtat tgtgctaaca
    38461 taatctgagt gtggaaacac taaaatcact atgatccgct gctatatatt tgtcgaggca
    38521 agaacatagg ttgaagagga gaatttataa tgacaaatca caaattaatc gaagcagtaa
    38581 ctaaatcaca attacgtaca gacttaccaa gtttccgtcc tggtgatact ttacgtgtac
    38641 acgtacgtat cattgagggt actcgtgagc gtatccaagt attcgaaggc gttgtaatta
    38701 aacgtcgtgg cggtggcgtt tctgaaacgt ttacagttcg taaaatttca tcaggtgttg
    38761 gcgtggaacg tacattccca ttacacacac caaaaattga aaaaatcgaa gttaaacgtc
    38821 gtggtaaagt acgtcgtgct aaattatatt acttacgtag tttacgtggt aaagctgcta
    38881 gaatccaaga aattcgttaa tcagcattta aacaaagcta tgtatgagtc aaattcgact
    38941 caaacaataa agccatctag gtcactttta tggagtgata tagatggctt tttttgctgt
    39001 ttagttaatg taaagttgat aatgtaattg tttattcctt ctctattcat aacgtgtttg
    39061 ctcaaaatgg ttatataaac aaaaatctac taagtgccat tgaaaaggac ttagtagatt
    39121 tattaaatat atgcgtagca acattatttt tgtcttgccc atctcgtgaa aataatacta
    39181 catataatgc caaatatagt aattgttata agtaaataat agtatggtgg ggtatagctc
    39241 aattgaatct ttgttatatt tttaggtgct ttaatgccgg tcataacacc atttacttgt
    39301 tcaactttaa gactttgatc acctgatgtc gctttcatac cttgattata tgctgttggc
    39361 aaaacaatat acccagatga atttttattt ttagtaatag tataaccatg ctttgtctta
    39421 ctaactttga cagcttctaa tgaatttgaa gcgtctttaa gcgtggtata atcttcgccg
    39481 tatatccctt ttaaatttac tcgatactta cctttaggca atgataatct aattctatct
    39541 ggagctttaa tgcgtatcgt tacgggtgtt acaacgcgtc gatatttata agtgagttta
    39601 tttctttctt gtgtatattc attcacttta acatcatgag ctttatccgg cgaaagtaat
    39661 tctaaatcca tttcaaaata caaatcttta aattgattag aaactgattt tggcaactgt
    39721 acagttagac caccattatt ttgtttaact tgtaataaat gttttgtagg agattgccag
    39781 gctgcactat ttaatttaat tgttgaatct gatagtaaat ttttattggc tttaaaatgt
    39841 gtattaacat ctttaatatt gttagaaaca atcccttgca acattgcttg ttctttatct
    39901 aatggagatt ttaattcttt attggaaaag accttatttg taatatgtgc acttggataa
    39961 tggatggtat ttttagaatg aatccaacga actttattgt ctttgtgttc agacttaatt
    40021 ttaaatccat atggtaagtt gtcatcatga ttcactctaa ttcgatcatt aacattccaa
    40081 agtgatagta aattttgacg attgccaagt aatctataag tgctgttttt atcgattggc
    40141 atattaattt ggagtgtctt gtcataatat tttaaaatgt ctccattaaa aatactagaa
    40201 tataatgaaa tgccattata atgatatata aatggtgaat ttaatgcata gtctgacata
    40261 taatcaatgc gattaaatga gcctgttgca ttttgattta tcttttttat aagctggttt
    40321 acatagttac tatggtaatc atgttgtttc aacgttgata atgattgttg ataaggtttg
    40381 attgccatgt ttttgttgtt atctaaaatg acgatttgtt gaatcataac gattaatact
    40441 aatattgcaa cggttaattt tttataacgc cataaactaa attttaaaat aacggcaagc
    40501 actattagca ggataatacc tactataagt gcaagtgggt gtgtcggtga tagtaataca
    40561 taaagtattg cgatgatgca tacgggtatt gttctgatta aataatattt catatttaat
    40621 gttgataaat gttgaataaa caatccgcaa agagcacttg atgatagtgc taagatatac
    40681 acccaacgcc tttctggaaa tgaaaaacca ttaaaagcac tgtcgaaata ctgtgataat
    40741 gaacctataa ataatatcca tgttactatt gcgaaaagtc tataaaagta aaaacgatac
    40801 agtttgaatg acaataatgc aacgatagta agaattgaaa tcgtaatata aaatccatcg
    40861 ctaaagaaaa aataatgata atcaagtggt gtcaaaaacg gtatatcaac attgggattt
    40921 tgctttctgt cattttccaa aaacgcagaa atgccagtga ataaaccaaa tacacttgat
    40981 aacacactca aaactgtagc agatatgacg caaattaatt tttgtgttct agagaca
//
LOCUS       DANRDI010000007        39133 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.007, whole genome shotgun sequence.
ACCESSION   DANRDI010000007 DANRDI010000000
VERSION     DANRDI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 39133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39133
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.007"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..482)
                     /locus_tag="PZ377_000219"
     CDS             complement(<1..482)
                     /locus_tag="PZ377_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDM6357112.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     T"
     gene            864..2126
                     /locus_tag="PZ377_000220"
     CDS             864..2126
                     /locus_tag="PZ377_000220"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDM6357113.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(2220..3494)
                     /locus_tag="PZ377_000221"
     CDS             complement(2220..3494)
                     /locus_tag="PZ377_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDM6357114.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            3666..4283
                     /locus_tag="PZ377_000222"
     CDS             3666..4283
                     /locus_tag="PZ377_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDM6357115.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            4411..5877
                     /locus_tag="PZ377_000223"
     CDS             4411..5877
                     /locus_tag="PZ377_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDM6357116.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            5984..7114
                     /locus_tag="PZ377_000224"
     CDS             5984..7114
                     /locus_tag="PZ377_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDM6357117.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(7075..7170)
                     /locus_tag="PZ377_000225"
     CDS             complement(7075..7170)
                     /locus_tag="PZ377_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357118.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(7264..8868)
                     /locus_tag="PZ377_000226"
     CDS             complement(7264..8868)
                     /locus_tag="PZ377_000226"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDM6357119.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(8855..10015)
                     /locus_tag="PZ377_000227"
     CDS             complement(8855..10015)
                     /locus_tag="PZ377_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDM6357120.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(10129..10575)
                     /locus_tag="PZ377_000228"
     CDS             complement(10129..10575)
                     /locus_tag="PZ377_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDM6357121.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            10655..11398
                     /locus_tag="PZ377_000229"
     CDS             10655..11398
                     /locus_tag="PZ377_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDM6357122.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(11614..12216)
                     /gene="rpsD"
                     /locus_tag="PZ377_000230"
     CDS             complement(11614..12216)
                     /gene="rpsD"
                     /locus_tag="PZ377_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDM6357123.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(12460..12924)
                     /locus_tag="PZ377_000231"
     CDS             complement(12460..12924)
                     /locus_tag="PZ377_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDM6357124.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            13061..14755
                     /gene="ezrA"
                     /locus_tag="PZ377_000232"
     CDS             13061..14755
                     /gene="ezrA"
                     /locus_tag="PZ377_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDM6357125.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKSDGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(14829..14969)
                     /locus_tag="PZ377_000233"
     CDS             complement(14829..14969)
                     /locus_tag="PZ377_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357126.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            15118..16266
                     /locus_tag="PZ377_000234"
     CDS             15118..16266
                     /locus_tag="PZ377_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDM6357127.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            16266..17489
                     /gene="thiI"
                     /locus_tag="PZ377_000235"
     CDS             16266..17489
                     /gene="thiI"
                     /locus_tag="PZ377_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDM6357128.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            17533..18303
                     /locus_tag="PZ377_000236"
     CDS             17533..18303
                     /locus_tag="PZ377_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDM6357129.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            18401..18895
                     /gene="tpx"
                     /locus_tag="PZ377_000237"
     CDS             18401..18895
                     /gene="tpx"
                     /locus_tag="PZ377_000237"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDM6357130.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            19018..19965
                     /locus_tag="PZ377_000238"
     CDS             19018..19965
                     /locus_tag="PZ377_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDM6357131.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            20053..21255
                     /locus_tag="PZ377_000239"
     CDS             20053..21255
                     /locus_tag="PZ377_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDM6357132.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(21501..22001)
                     /locus_tag="PZ377_000240"
     CDS             complement(21501..22001)
                     /locus_tag="PZ377_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDM6357133.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            22142..23260
                     /gene="ald"
                     /locus_tag="PZ377_000241"
     CDS             22142..23260
                     /gene="ald"
                     /locus_tag="PZ377_000241"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDM6357134.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            23384..23482
                     /locus_tag="PZ377_000242"
     CDS             23384..23482
                     /locus_tag="PZ377_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357135.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(23576..24631)
                     /locus_tag="PZ377_000243"
     CDS             complement(23576..24631)
                     /locus_tag="PZ377_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDM6357136.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            24806..25495
                     /locus_tag="PZ377_000244"
     CDS             24806..25495
                     /locus_tag="PZ377_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDM6357137.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(25800..26213)
                     /locus_tag="PZ377_000245"
     CDS             complement(25800..26213)
                     /locus_tag="PZ377_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDM6357138.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            26418..27716
                     /locus_tag="PZ377_000246"
     CDS             26418..27716
                     /locus_tag="PZ377_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDM6357139.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            27977..28918
                     /gene="pde2"
                     /locus_tag="PZ377_000247"
     CDS             27977..28918
                     /gene="pde2"
                     /locus_tag="PZ377_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDM6357140.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            28939..32136
                     /locus_tag="PZ377_000248"
     CDS             28939..32136
                     /locus_tag="PZ377_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDM6357141.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
     gene            32586..33815
                     /locus_tag="PZ377_000249"
     CDS             32586..33815
                     /locus_tag="PZ377_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDM6357142.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            34010..34867
                     /locus_tag="PZ377_000250"
     CDS             34010..34867
                     /locus_tag="PZ377_000250"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDM6357143.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            34867..35811
                     /locus_tag="PZ377_000251"
     CDS             34867..35811
                     /locus_tag="PZ377_000251"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDM6357144.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            36080..37048
                     /gene="pfkA"
                     /locus_tag="PZ377_000252"
     CDS             36080..37048
                     /gene="pfkA"
                     /locus_tag="PZ377_000252"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDM6357145.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            37070..38827
                     /gene="pyk"
                     /locus_tag="PZ377_000253"
     CDS             37070..38827
                     /gene="pyk"
                     /locus_tag="PZ377_000253"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDM6357146.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT        13443 a         5568 c         7245 g        12877 t
ORIGIN      
        1 gtaagaactt gatttttaac aagttgttgt gtaattgtgc taccacctga actttgatca
       61 gtattaaaaa tatcttgtat cattgctctt aaaatcgcct ttggtaagat gccatcatgt
      121 ttataaaata aagtgtcttc agatgacgtt aatgctttaa tgacatttgg acttgatgtt
      181 ttagggccta taatgagtga gttttgagaa tggttatact catataataa atttttgtta
      241 ttatgatcta ataattcatc gccaggtatt tgtcgaactt tttttattaa agcatcatct
      301 gataatgagt cggacgtttt agttaaatga tgaaaatata aagacatcgc aatcacagcg
      361 atagcaacga tggcaatgaa aatcataaat ataaaaacaa tgacatgttt gatagcttta
      421 tatatctttt caaaatgata tatacgatga ttcaattgat gattgttgtc ttgattcgtc
      481 atctttaacc tcctaaatta cgtatagtat accatattta agcaatttga cttttatttt
      541 aacgttaatt ataattaggg aaaatgcata aagaatagga taaaaggaca agtaatatta
      601 tttgaaatca tcagagagct agtggtcgct gtgaactagt gtttttaata atatgaatac
      661 acctttataa ctaacatgtc ctacgtttat taatgaacgt atcattaata gagtgaacat
      721 tatcttatga ttatgtttaa gtttggtggt aacgtgcaat cgcgccctta catatttgtt
      781 gtaagggttt tttttacgtt caaaaaactt taaagtattt taacaagtat tcaataaata
      841 attataaata ggaggaaata ataatgacga atgtattaat tgaagattta aaatggagag
      901 gtcttattta tcaacaaact gatgaacaag gtattgaaga tttattaaat aaagaacaag
      961 tgacgttata ctgcggtgcc gatccaacgg cagatagttt acatattggt cacttactac
     1021 cattcttaac attaagacgt tttcaagaac atggacatcg tcctatcgtt ttaattggcg
     1081 gtggtacagg tatgattggt gatccatcag gtaaatcaga agaacgtgtg ctacaaacag
     1141 aagaacaagt agataaaaat atcgaaggta ttagtaagca aatgcacaat atttttgaat
     1201 ttggaacaga ccatggtgca gtgcttgtta ataatagaga ctggttagga caaatctcat
     1261 taattagttt tttacgtgac tatggtaaac acgtcggcgt taattacatg ttaggtaaag
     1321 attcaatcca aagtcgttta gaacatggta tttcatatac agaattcaca tacacgattt
     1381 tacaagctat tgatttcggt catttgaata gagaattgaa ttgtaagatt caagtaggtg
     1441 gatcagatca atggggtaat atcacaagtg gtattgaatt aatgcgtcgt atgtatggtc
     1501 aaacagacgc atacggttta actattccgc ttgtaactaa atcagatggt aagaaatttg
     1561 gtaagtctga gtcaggtgct gtttggttag atgctgaaaa aacaagtcct tatgaatttt
     1621 atcaattctg gattaatcaa tcagacgaag atgtaattaa attcttaaaa tactttactt
     1681 tcttaggaaa agaagaaatt gatcgcttag aacaatctaa aaatgaagca ccgcatttac
     1741 gtgaagctca aaaaacatta gctgaagaag taactaaatt tattcatggt gaagatgcat
     1801 taaatgatgc aatccgtatt tcacaagcat tatttagtgg tgatttaaaa tcattatcag
     1861 cgaaagaatt aaaagatgga tttaaagatg tgcctcaagt gacattatca aatgacacaa
     1921 caaatatcgt tgaagtcctt attgaaacag gcatttctcc ttctaaacga caagcacgtg
     1981 aagatgttaa caatggtgcg atttatatta atggtgagag acaacaagat gttaattatg
     2041 ctttagcacc agaagataaa attgatggcg aatttacgat tattcgtcgc ggtaagaaaa
     2101 aatacttcat ggttaactat caataaatat aattgcatag ctaaataaat tagagcctac
     2161 tcatattcat tcctaagaat gtaatgagta ggctcttaat gtacttttct gtctgtaaat
     2221 tatctaaaga aatctctatc gccttggcca ggtgattgac gttctgatcg gctttgacgt
     2281 tttggttgtt ctttttgttg ttttaatttc actttaactt cttttgtttt accatcacgg
     2341 ataacggtaa cagtgactga ttcaccaggt tttttatttt catataaata gcttcttaaa
     2401 tcaacatcat ctttaatttt cttgccatca atttctgtaa taatatcacc ttttttaaga
     2461 tcaatatcac tatcagcttt ggcgacataa ataccgtctt ctctatcagt atgaagttgc
     2521 tcgcgctctt cttcaggaat atctttcaaa ttaattaaac caataccaat cgaagggcgg
     2581 tcaattttac catgttttac aagttgttca attgttactt taacttcatt acttggaata
     2641 gcaaacccga taccttcaac ttgtgtcgca gcaattttca ttgagttaat accaactaaa
     2701 ttaccattaa tatctactaa tgcgccacct gagttacctg ggttaatagc agcatctgtt
     2761 tgaagaacgc taacttttgt attgccacca gttgtctcag cgtcaatcgt acgttcgctt
     2821 gctgaaatga taccagatgt tacagagtta gcaaattgta atcctaatgg gttacccatt
     2881 gcgaatacgc tatcgccagt ttgtactttt gaagagttgg caaattgaat cgctttaata
     2941 ccttttgtat tttcaatttt aagtacagca atatcagtta ctgcatcttt accaactaat
     3001 ttcgctttaa cttgtttttt attatgtaat tggactctaa tttcatttgc gccatcaata
     3061 acatgattgt ttgtaacgat ataagctgaa ttgttgttta tttgatagat aacacctgaa
     3121 cctactccag cttcagatgg tttagatgat ttgcctttta ataagtcgtc tacacttgat
     3181 gctttttgca tgttaataac tccaacaatt gtaggggaga cagattttat catttcatga
     3241 acggtaccga atttcttgct ttgaccgtct aattgattgc cacctttatt atttgttgtc
     3301 tgaacagttg aaccatcttt atttaaaatt gtactattta atactttgcc tataccaagt
     3361 actagaagtg caccaataat tccagcaatc aatgcaacga tgactgtttt aaaccatgga
     3421 aatttaggtc ttctgtatct aggtgtttgg ctatggtttg ttgtagaatg atctgtatga
     3481 ttaaaatctg acatacttaa cctccattat atgatttata tatgctttaa ttatgtcttt
     3541 tttttataat tattgctagt aatacactat acaactttag atgtattttc agactatctt
     3601 gttctagtaa aaatatagtg ataaaatcta gttgttataa acaaataagt taagtaggtg
     3661 gcacaatgta ttcagtgatt agtaagattt tgaatttcat tttagttaaa atgtcaaaat
     3721 cattatacgt aatcggtaaa gataatattc ctaaggatag taaatatgtc gtaacttgta
     3781 cgcatgaaag ttataacgaa gtcattatgc taggtatggc attatatcct aatcaaattc
     3841 attatatggc taaaaaagaa ttattcaaaa ataaatggat tggtaaattt ttaacgtctt
     3901 taaatgcatt tcctgtagat cgtgaaaatc caggacctag tactttaaaa cgcccaatca
     3961 atttgttgaa agataataag acagttggta tcttcccaac aggttctcgc acatcgcaag
     4021 aaggtgcacc tttgaaacgt ggtgcttcaa caattgcaat gttaagtaag tcaccaatat
     4081 tacctgttgc ttatgttggt cctactaaga ttcacggtct tttaacagga caagcataca
     4141 taaatattgg caaaccaatc gatattaacg atttacctaa agatttaaaa cgaaacgagc
     4201 gtatcgatta tattacaaaa gagattgaaa cacgaacagc aaagttacaa caagaattgc
     4261 atgaaattgt aaaaagttta taaaataact tgaggtagaa atatagctgg ttagtaagcc
     4321 taggtgacta taacggagta tcgaagtagt tattctagtt acattagtaa tctttatatt
     4381 tctacattat tttaagaagg atgacattca atgtataaat ttttccaaaa tttagggcgt
     4441 tcgttaatgt taccagttgc cattttaccg gctgcagcaa ttattgccgg aattggtaat
     4501 acgttaaatg cacttcatgc gacgccgaaa atcgcaatgt tctttactac ggtaggtact
     4561 acgattttag agcaattagg tattttattt gcaattggtg tagctatagg aatggcaaag
     4621 aaaaatgatg gtgtagtcgc gcttgcagct acgttaggat attttttagt tacagttgta
     4681 ttatcaccaa tgaaattagc gcctttatta ggaatgaaag cttcggaaat aaattcagct
     4741 tttgaaaaga tgaataatgg caatgtcttt gttggtattg taataggttt aatagcagct
     4801 tatgcataca ataagtttag tgaaacagaa ttaccattag cattatcatt ttttagtggt
     4861 aaacgtctag ttccaattat gactgcattt tactgtacat ttttagttgt catattgtta
     4921 ttcttatggc cactacttta ttcatggatt gtaaaatttg gtgaatctat agtcggttta
     4981 ggatcgtttg gagctttcat atatggtgtt gccaatagat tattaattcc tacaggatta
     5041 catcatgcac tgaatagtgt gttctggttt gatacgattg gtattaatga tattggtaaa
     5101 ttccaaagtg gcaaagatgc tataaaaggt attacaggac gctatcaagc gggattcttc
     5161 ccaatcatga tgttcggtat tccagcggct gcgttagcaa tgtatcatac agctaaaact
     5221 actcagaaaa aacaagttta cggttggttc ctagcgagtt cagttgcagc atttttcgtt
     5281 ggtgttacag agccgattga gtttgcattt atgtttgttg cgccaatctt atatgttgtt
     5341 catgcgttat taacaggatt atctttattt atcgcagcta cattccattg gacagctgga
     5401 ttttcattta gtgcaggttt gatagattat gtgttatcac taattaatcc agtatcaaat
     5461 catccattga tgttgcttgt tcaaggtgtt gtatttttca tcttatacta tgtgattttc
     5521 cgtgtagtaa tccaagtatt taacttgaat acgattggta gaggtgaaaa tgaattagtt
     5581 gacccaacag ttgtaaaaga taatattgct cctggtgaaa atgatattaa acaaagtaaa
     5641 tatcatcaac atgctataca aatattagaa ggtttaggcg gtcaagagaa tattgttaat
     5701 ttaaccaatt gtgcaacaag gttgcgtcta gagttaaaag acacatctat tattgataaa
     5761 cagaagatta aaaacgccgg tgcagttggt gttactgtta atggcaaaca ttcaactcaa
     5821 gttattgtcg gtacacacgt acagcaggtt gctgatgaaa ttgaaaaaca tttataaaat
     5881 ttaaacagct aaagcgcaaa tctaacttca atagggtttg cgcttttttt agtcaaatag
     5941 tagaatgatt tatatctgaa ttttcagaaa tggagtgggg attgtgaaga aaattttatt
     6001 tgatgttgat ggcgtttttt taagtgaaga acgttgtttc gatgtctctg cattaacggt
     6061 atatgaatta ttaatggata agtgttattt aggtcttcat tcacatatag attgggaaac
     6121 tttgactgat aacgatatac aagacattag gaatcgaatt tttcaaaaag ataagatttt
     6181 aaacaaatta aaatcgctag ggctgaattc aaattgggat atgttattta tcgtttttag
     6241 tattcattta attgatattt tgaaaaagtt atcacatgat gaaattgagg cattcatgta
     6301 tcaagatgag cctgtcgaat tgaagttgca aaatataagc acaaacttag cagactgttt
     6361 taatttaaat gaacaactac ctttacaatt tttggataat gtaaaagttg gtaaaaataa
     6421 tatttatgct gctttggaag agtttgcaac aacagagtta catgtttcgg atgctacttt
     6481 atttagtttg aagggtgcat tatggacgtt agcgcaagaa gtttatcaag aatggtattt
     6541 aggatcgaag ttgtatgaag atgttgaaaa gaaaatagca cgaactactt ttaagacagg
     6601 ttatatttat caagaaatta ttttgagacc agtagatgaa gttaaggtac ttctgaatga
     6661 tttaaaaggt gctggtttcg aattaggtat tgcaacaggt cgtccttata ctgagactgt
     6721 tgtgccattt gaaaatttag gattgttacc atattttgaa gctgatttta ttgcaacagc
     6781 aagtgatgtt ttagaagcag agaatatgta tccgcaagca cgaccattag gaaagccgaa
     6841 tccttttagt tatatcgcag ctttatatgg taataatcgc gataaatatg aatcttatat
     6901 caataagcaa gataacattg taaataaaga tgacgtattt atagtaggcg attcgttagc
     6961 tgacttatta agtgctcaaa aaataggtgc aacgtttatt ggaacattaa caggtttaaa
     7021 aggtaaggat gctgcaggtg agttagaagc gcatcatgcc gactatgtta ttaatcattt
     7081 aggtgaactt agaggtgtac tagataattt gtaatttgat tgttgtttga cagcataact
     7141 tgtagtgaat gattgaacca aaggtttcat attgagttac aatgaaatta ataatgaaaa
     7201 aatgccaaga agcaatggaa gtaatccaat gtcttcttgg cattttgaat ttacataaat
     7261 tgtttatgac tgtaccgtca attcagttgt gaaaatttga ttgtattcac caacttgttt
     7321 aagttcatca attatattgt ttgaaacagg ttgatcaacg gataaaatca ttagcgcatc
     7381 tccgcccgct tcagttctac ctaaagtcat agatgcaatg ttgatattgt atttacctaa
     7441 caatgcgcca gtttttccta ccatacctgg agtatcatta tgatatgaca caatttgata
     7501 ttgatttggc tttaagtcta cagaaaaatt attaattcta acaattcttg gaccgaaacc
     7561 tgtaaagaca gaagcgccaa ctttaacgga atcgctatcg tttgatagtt ctacctctaa
     7621 gtagttacta aaacctgtct ctgctttatt attttcaata tttaatgtca cttgttgttg
     7681 atttaacaac attagagcat tgataatatt gacttcagga ccaagatcat cttttaaaat
     7741 atgagtaata attgtacgtg ttaataaact actatcaata gaggctaagt cgccaccgta
     7801 cgtaatttta atagagcttg gtgcattgta cattaattga atagctaatt caccaactgt
     7861 ttggcttaaa ttgatgaatg attttacagt atcatctata ttgcttaagt ccattttagg
     7921 tgcattcact gcatgcgtta cagtaccatc aattaaaatt tcgatgattt catttgaaac
     7981 agaaattgcc actttttctt gagcttcgac tgttgaagca cccaaatgag gtgtaacaat
     8041 aattttatca tgtgcaacaa gaggcgagtc agttgcaggt tcatgttcaa acacatcgat
     8101 agctgcccga ctaatttgtc cttcgtctaa tgcttttatt agcgcctttt catcaataat
     8161 accaccacgt gccacattga ttatttgcaa actaggtttt gctttggcaa aaaagacagc
     8221 attaattaag ccttttgttt taggtgttag tggtgtatgt aatgtaacga aatcagaatg
     8281 ttgggcaatc tcatcaactg ttgccttcgt aatgcttaaa gattttgctt tttcatccgt
     8341 taagtaaggg tcaaaagcta gtattttcat tccgaaactt tgcgcacgtt tagcaacacc
     8401 taaaccaatt ctaccagcac caatgacacc taatgtttta tgataaagct cagtaccttt
     8461 aaatgcattt cgattccatt ctttatttgt aagtgattgg tgtgcttgcg gaatatttcg
     8521 tgccattgat aataacattg ccagtgtatg ttcagtagct gaaatcgtat taccatctgg
     8581 ggcattaata actaaaatac cttttaatgt tgcagcatca atattaatat tatctacacc
     8641 aacaccggcg cgtgcgatta cttttaaaga atcagcagca tttatgatat tttcagtaac
     8701 cgtagtttga ctacgaacga ttaaagcatg gtatgaaggt ataattttga ttaatgcttc
     8761 ttcggacaag ccagtttgaa tatctacatt gaattgttcg tgatctaata atgctttgat
     8821 accatctttt gatatgggat ctgcaacgag tacattaaat tgcttcatga ataacctcca
     8881 tatattttga tataccttta ccgatatagt taactttacg gtgttcagtt aaaataattt
     8941 ctaaagcaga tactaccgat aaaatatcga aaggactaat tttccccata tgaccaattc
     9001 ttaaaatttg acctttaaga tggccttgac caccagcaat tgttatttta aagcgatttt
     9061 taagttcatc tttgattatt ttcacttcat catttgtatt aggtttgaat gctgtaaccg
     9121 ttggagatgc atctttatca ttgactaata aagttaaatc taatgctttt aaggcgcttc
     9181 ttaatgcatt tctaattgca tagtgtcgtg ctattacgtg attgaaacct tctgctttta
     9241 cggtttcaac gtatgcattt acacctctaa ataaagacac atttggtgtg aacggtgtag
     9301 aattgtcagc ttgtgacgaa atgtatttat ttaagtctag ataaaatttt ggcgtagtta
     9361 cttctttgaa atgttctttt gcacggtggc tataagctac aaaagctaat cctggaggta
     9421 acataattgc tttttgacta ccagaaacaa gtacatcaat tttatctttg ttaatgtcaa
     9481 catcaacagc accaatgcaa cttacgccgt caactacaaa ataaatatta ctattaaatt
     9541 gattaatggc atttcctaac tcgtgaatag ggtgtagcac tgtcgtagat gtttcgcaat
     9601 attgactaaa tactgcttta acttcaacat ttaaagttga aaggaaattg atgaaatctt
     9661 tgacatctac agcttctccc catgttacgt cataaatatg cacatttttg taataagttt
     9721 gtgcaatttg tttaaatcgg ttaccaaagg cacctgaaac aatgacaacg aagtgatctt
     9781 cagggtttac aatgttcaac atactagcct ccaagacgct tgtaccgcta gatgttaaaa
     9841 taagtacatc attttgactc ccaaatattg gctttagacc ttgaaatgct tgttgtgcga
     9901 tgtcttcaaa atctttagaa cgatgaccaa ccataggtgc ttgaatttct ctcataatgg
     9961 catcaggtac aggggttggg ccaggtgtta ataacaacgg ttgatgataa tacatttata
    10021 tgcctcctat aaataagatt tcactatttt agcaaatttt ctgaaaattt aaaagcctaa
    10081 aaaagtgata acgctatatt gtcgaacaaa cgttatcact taacgatttt atttggcctg
    10141 aatgatggga tagattttta tgtccacatt atttcttaca gcatttgaaa tcatgcaatt
    10201 attatctgca attgttatca attttggtaa tcgcttttct aattgtgcta tttgatcact
    10261 tggaatttga atttgaggat ggtgcacaat ttttgacata ctgaattttc cgttatttaa
    10321 acaagctgtt ccaatcgatt gttgttcaat tgaaatatct gtgaactttg cacgttcaag
    10381 agtagctgct aatgagatga tataacatga tgaagcggct gatactaaca tttcatcggg
    10441 atttgttcct ataccaacac cacctaaaga agcaggtata gaaatattct ctgaaagtat
    10501 gtcgccttga acgtttccga cattgttacg accaccttgc caagaagttt ggactttaaa
    10561 gtcatgttga tgcaattgct ttaacctcca atataattgt gatagtttaa ttttagaata
    10621 gtttatcaaa gtttaacaga aaggtgactt atcaatgact ctgaataaac tgaaagatga
    10681 attacaaatt gtttcgcacc gtggattgcc gagtgatttt cctgaaaata caatggtcgg
    10741 ttatcgagag gtaatggggc tcaatgttgc tatgttagaa atagatgttc atttgaccaa
    10801 agaccaacat tttgttgtga tacatgatga aacaattgat agaacatcgg atggtaaggg
    10861 gcgtattgct gattacacat tatcgcaatt aaaatcattt gattttggta gttataaaga
    10921 tgttgctttt aagggagaac gaatacccac gttagatgaa gtactttcat tatgcttaaa
    10981 gtatgataaa aagctattga ttgaattgaa aagtccgaat ttatatccag aaatagaatg
    11041 taaattgctg gcatttttgg aagaaaagaa agtcgatgcg acacaagtag ttatacaatc
    11101 ttttgatatc gaatgcatcg aaaagttaaa cacattgggc agtatatatg aattaggtgt
    11161 gctttgtagt aaacgtaatt attggtataa aaagccaaac ttttcaagaa ttgctcaaat
    11221 tgctagttat gttaatccta attatgcatt agtgaccaga aaatttgttg ataaagcaca
    11281 tcatcatcaa ttacaggtta tgccatacac agttaataaa ttgaaaactg gagaaaaatt
    11341 aagacaaatg ggtgtagatg gattaattac agataatccg aaattattta ttaagtgaga
    11401 cgatgaatgg taatttgata gagggatgag gattcgtata tcgcaattga taattttaaa
    11461 aactaatgat gaaatactaa agataattaa acaaataaga cttgtaaatt ctaagttgat
    11521 tgtaaaatag ggtagctcca tataattttg aatctatcat ttttaaatta ataaaaaaaa
    11581 cacccacaat tgtgggtgtt tgtattgaac gtattatctt gagtagtact caacgattaa
    11641 ttgttcgtta atttcagcag gtaattcgct acgttctggt aaacgtacga aagtaccagt
    11701 taagctgtca gcatcaaagt ttaagtactc aggtacgaaa ttgttgattt caactgattc
    11761 aacgatgatg tttaattttt gagatttttc acgaactgaa attgtttgac caggtttaac
    11821 agaataagat ggaatatcaa cacgtttacc atctactaag atatgaccgt ggttaactaa
    11881 ttgacgtgct tgacgacgag tacgagctaa acctaatgaa taaacaacag cgtctaaacg
    11941 acttgctaat aagatcatga agttttcacc gtgtacaccg aattttttac cagcgatgtc
    12001 aaatgtgtta cggaattgtc tttcagtcat tccatataag taacgtaatt tttgtttttc
    12061 acgtaattgt aaaccatatt ctgataattt tttacgttgg tttggaccat gttgtcctgg
    12121 tgcgtaagga cgtttttcta attctttacc agtaccgctt aaagagatac ctaaacgacg
    12181 agattttttc cagtttgaac ctctgaatcg agccataata agactcctcc tttaattctt
    12241 ttttgttgtt atgaataaac aaaaaagagt gttgcatgct tactaagata tattgtttta
    12301 tgtgtcttca ccttatagcg acagttacgt gacacgtccg cattgggaac aacatagcgc
    12361 tcaataatat acaactgcta ctttcgttaa ttcacacaaa gattattgta acattttcct
    12421 gctaactgtc aacactttat tgaaaatgct gatgtccttt tatgcgagtt gcttttcaat
    12481 aattttaaca attgcttcaa gatgttcttt gtcattgtca tcaaatcgat ccgttatagg
    12541 ggcatcgata tctaagacgc cgataatttt atcatctttg aaaattggaa cgacaatctc
    12601 agacttacta ttagcatcac aagcgatatg tccttcgaat tgatgaacat cagctacaat
    12661 ttgtgtacga cgttctgaaa cggctgtacc acatacacct tttccaattg gaatgtggac
    12721 acaagcgggg tgtccttgga aaggtccaag tataagttca ttttgttcca ataagtaaaa
    12781 gccaacccaa ttaatttgat ctagattatc atttaataag gcagacatgt tacttaaaat
    12841 agcaatcatg tgatgttcat cttcaataag gcttgctgct tgtttcttta ataatgtgta
    12901 gtttgttggg ttaattgttg tcatatttaa aacctcttat tttcatagat tttataatag
    12961 tatagaattt cgaaaatatt atggcaataa attcatttta taatttatcc ttaagcaaaa
    13021 ttacgttata atagtaatga taaattagga ggagaagcat atggtgttat atatcatttt
    13081 ggcaataatt gtgattatat tgattgctgt aggtgtatta ttctatttac gttcaaataa
    13141 acgacaaatt attgaaaaag caatcgaacg taaaaatgaa attgagacgt taccttttga
    13201 tcaaaacctt gcacaattat ctaagttgaa tttaaaaggt gaaacaaaaa cgaaatacga
    13261 tgcaatgaaa aaggacaacg tagaaagtac aaataagtat ctagctcctg tggaagaaaa
    13321 aatccataat gctgaggctt tattagataa atttagtttc aacgcatctc aaagtgaaat
    13381 tgatgatgca aatgagttga tggatagtta cgaacaaagc tatcagcaac aattagaaga
    13441 tgtaaatgaa attattgcgt tatacaaaga taatgatgaa ttatatgaca aatgtaaggt
    13501 tgattatcgt gaaatgaaac gtgatgtttt agcaaatcgt catcaatttg gtgaggcagc
    13561 aagtctactt gaaactgaaa ttgaaaaatt tgagccaagg ttagagcaat atgaagtact
    13621 aaaatctgat ggtaattatg tacaagcgca caaccatata gctgccttga atgaacaaat
    13681 gaaacagcta agatcttata tggaagaaat accagaatta attagagaaa ctcaaaaaga
    13741 attacctggt caattccaag atttaaaata tggttgccgt gatcttaaag ttgaagggta
    13801 tgatctggat cacgtgaaag tagacagtac attacaaagc ttaaaaacag agcttagttt
    13861 cgttgaacca ttaattagcc gcttagaatt agaagaagct aatgataaac tagctaatat
    13921 caatgataag ttagatgaca tgtatgattt aattgaacat gaagttaaag ctaaaaatga
    13981 tgtcgaagaa acaaaagata tcattacgga taacttattc aaagctaaag acatgaatta
    14041 tacattgcaa acagaaattg aatatgtacg tgaaaactac tatataaatg aatctgatgc
    14101 tcagagtgtt cgtcaatttg aaaatgaaat tcaaagttta atttctgtat atgatgatat
    14161 tttaaaagaa atgtctaaat ctgctgtgcg atatagcgag gttcaggata atttacaata
    14221 tttagaagat catgtcacag ttattaatga caaacaagaa aagctacaaa atcatctgat
    14281 tcaattgcgt gaagatgaag cagaagcaga agacaatctg ctacgagtac aatcgaagaa
    14341 agaagaagtg tatcgtcgat tacttgcttc taacttaaca agcgttcctg aaaggtttat
    14401 catcatgaaa aatgaaattg atcatgaagt tcgtgatgtt aacgaacaat ttagtgaacg
    14461 tccaatacac gttaaacagt taaaagataa agtgtctaaa attgtgattc aaatgaatac
    14521 atttgaagat gaagcaaatg atgttcttgt taatgctgtt tatgcagaga aattaattca
    14581 atatggaaat agatatcgta aggactatag caatgttgat aagagcttaa atgaagctga
    14641 acgattattt aaaaataatc gctataagcg tgcgattgaa attgcagagc aagctcttga
    14701 aagtgttgag ccaggtgtta ctaaacatat tgaagaagaa gttattaagc aatagaaact
    14761 agtatgtagt tatacttaaa taatatgagc actctgtcaa attggactga tgagtttaat
    14821 aattgaagtt agccaacgat acgttgtcta gcttcttttt tatatggata aatgaaaggg
    14881 acaataaata taaatagcaa ttgtttaaag ataaacgtaa tcaaatgtgt tgttttaatt
    14941 aatataagta gtgaaaaaag cataatcaca cagctgttta aatagagtga aatagtctaa
    15001 ttcttattta ataagtagaa ataagattat tttgcgctat aacctttatt ttttgtcgga
    15061 taatagtata atgcttgagt aaaacaattt taacgattga tgattaatga aagagaagtg
    15121 tttattttga tatatctaga taatgcggca acgacgaaag catttgaaga agtgttagat
    15181 acttatttaa aagtaaatca atcaatgtat tataatccga atagtccgca taaagctggt
    15241 ttgcaggcaa atcaattact acaacaagca aaaacccaaa ttaatgcaat gattaattca
    15301 aaaacaaatt atgatgttgt attcactagt ggtgcaactg aatccaataa tcttgcttta
    15361 aaaggtattg cctatcgtaa atttgataca gcgaaggaaa taattacatc cgtgttagag
    15421 catccgtccg tattagaggt tgtaagatat ttggaagcac acgaaggatt taaagttaaa
    15481 tatgttgatg taaagaaaga tggcagtatt aacttagaac acttcaaaga attaatgtca
    15541 gacaaagtcg gtttagtaac atgtatgtat gtaaataatg taactggaca aatacagcct
    15601 attccacaaa tggctaaagt tataaaaaat tatcctaagg cacattttca tgtagatgcg
    15661 gttcaagcat tcggcaaaat ttcaatggat ctcaataaca tagatagtat tagtttaagt
    15721 ggacacaagt ttaatggttt aaaaggacaa ggcgtcttac ttgtaaatca cattcaaaat
    15781 gttgaaccaa ctgtccatgg tggtggtcaa gaatatggcg ttagaagtgg aacagttaat
    15841 ttgccaaatg atattgcaat ggttaaagcg atgaagatag ctaatgaaaa ctttgaagca
    15901 ttgaatgcat ttgttactga gttaaataat gacgtccgtc aatttttaaa taaatatcat
    15961 ggagtttata ttaattcttc aacttcaggt tcaccattcg ttttaaatat tagttttcct
    16021 ggcgtaaaag gtgaagtatt agttaatgct ttttcaaaat atgacattat gatatctacg
    16081 acaagtgctt gttcatctaa acgtaataaa ttaaatgaag tattggctgc aatgggatta
    16141 tcagacaaat ctattgaagg tagtataaga ttatcatttg gggctactac aactaaagaa
    16201 gatatagcga ggtttaaaga aatatttatc atcatttatg aggaaattaa ggagttgcta
    16261 aaataatgaa gtatgatcac ttgcttgtta gatacgggga gttaacatta aagggttcaa
    16321 atagaaagaa atttgtaaat caattaagaa ataatgtaaa taagtcatta aaaggacttg
    16381 atgggtttgt cgttaaaggc aaacgagatc gtatgtatat tgaacttgaa gaccatgcag
    16441 atataaatga aataacatat cgattatcaa aaattttcgg tattaaatct attagtccag
    16501 tattaaaagt agaaaaaaca atagaggcaa taagtgcagc ggcaattaaa tttgcgcagc
    16561 aatttgaaga aaacagcaca tttaaaattg atgtgaagcg tgccgataaa aatttcccaa
    16621 tggatacgta tgaattacag cgtgaattgg gtggtgcagt attgaagcac ttcgacaata
    16681 tttcagtgaa tgtcaaacgt ccagatcatg aaattcgagt ggaagttaga ttagatgcaa
    16741 tttatatgta tgaagaagtt gttccgggtt caggtggatt accagttggt actggtggta
    16801 agacgttact aatgctttca ggcggtatag actcaccagt tgctgggatg gaagtgatga
    16861 gacgtggcgt aacaattgaa gcgattcatt tccatagtcc accatttaca agtgatcaag
    16921 caaaagaaaa agttattgaa ttgacacgta ttttagctga acgtgttgga ccaattaaat
    16981 tgcatattgt accatttaca gaattgcaaa aacaggtaaa taaagttgta catccaagat
    17041 atacaatgac ttcaacgaga cgtatgatga tgcgtgttgc tgataaatta gtacatcaaa
    17101 taggggcttt agctattgta aatggtgaaa acctagggca ggtagccagt caaacacttc
    17161 atagcatgta tgcaattaat aatgtaactt ctactcctgt attacgtcct ttattaactt
    17221 acgataaaga agaaattatt attaaatcga aagaaattgg tacatttgaa acatctattc
    17281 aaccatttga agattgttgt acaattttca cccctaaaaa tccagtaacc gaaccaaact
    17341 ttgataaggt agtccaatat gaaagtgtct ttgattttga agagatgatt aatcgtgctg
    17401 ttgaaaatat tgaaacactt gaaataacta gtgattataa aactattaaa gaacagcaaa
    17461 caaaccaatt aataaacgac tttttataaa taaaatccta gagtaaattt aaacataagg
    17521 ggatgttaaa ctatggattt gaacttaacg atgattataa tcataatttt atttggtttt
    17581 atcgcggcgt ttatagattc ggttgtaggg ggtggcggtt taatttctac gccagcatta
    17641 ttagcaatcg gtctaccacc atctgtggct ttaggtacaa ataaattggc aagttcgttt
    17701 ggttctttaa ctagtacgat aaagtttata aggtccggta aagtggactt atatgttgtt
    17761 gccaaattat ttggttttgt atttttggca tctgcatgtg gcgcatatat tgcaacgatg
    17821 gttccgtcac aaatattgaa acctttaatc atcattgcac tttcgtcggt gtttatattc
    17881 acattactta aaaaagattg gggcaataca cgcacgttta ctcaatttac atttaagaaa
    17941 gccataatat ttgcagcact ttttatatta atcggctttt atgatggatt tgtaggtggg
    18001 ggaacaggtt catttatgct ttttgtattg ttagtctttg gttttgattt tttaagtgca
    18061 gcaggaaatg ctaaagtttt gaactttgct tctaatatag gtgcgcttgt attatttatg
    18121 gtattaggac aagtagatta tgtaataggt ttaattatgg ctataagtat gattgctggg
    18181 tcatatgctg gggctcattt tgctattaaa caaggtgttg gttatgtaaa agtattattt
    18241 attattatta ctgctatttt aattttaaaa aatgcttttg attatattca acaatttgta
    18301 taaaattaaa tttgcttttg attaaagcat gaaaattgta atcaaaccat aaattgtcgt
    18361 atgatgtagt tagaatttta aaatgcagga ggtcaagtat atgactgaaa taacattcaa
    18421 aggtggacca atccacttaa aaggtcaaca aattaatgaa ggtgattttg cacctgattt
    18481 tacagtgtta gataatgact taaatcaagt aacattagca gattatgctg gtaaaaagaa
    18541 attaattagt gtggtaccat caattgatac aggtgtttgt gatcagcaga ctcgcaaatt
    18601 caactctgat gcttctaaag aagaggggat tgtgcttaca atttcagcag acttaccatt
    18661 cgcacaaaaa agatggtgcg cttcagcagg tttagacaat gtcattacat taagtgacca
    18721 ccgtgactta tcatttggtg aaaactatgg cgttgttatg gaagaacttc gcttattagc
    18781 tcgtgcagta tttgtattag atgcagataa taaagttgtt tataaagaaa tcgttagtga
    18841 aggtactgat ttcccagatt ttgatgctgc tttagctgca tacaaaaata tttaatcatt
    18901 aaagagataa atcttaaaat gtatacatcg tgtccatcgt tgtcaacagc attaaaatag
    18961 aattgttttc tatgattgct aagacctatg ggcacttttt attggagagg gacgaatatg
    19021 gcagaacaac aaacaattat ggaacgcttg tttcatacat tagatgaaaa agctaaaaca
    19081 ttaaataatg aaaatggcca aagttttatt gaaaatcttg ggctagcaat ggaacaagta
    19141 tataccaatg aaagaggatt gttagaacaa tcgacgttac aagatcgacg taaagcattc
    19201 caatttgcat atttaagttt aatgcaggaa gaaaagatac aagcaaatca tcaaattaca
    19261 ccagattcaa ttggattgat actaggattt ttagttgagc gttttatgaa caaccaagaa
    19321 gaattacata ttgttgatat tgcaagtggt gccggtcatt taagtgctac tgtaaaagaa
    19381 gtgttacctg aaattgcggt tatgcatcat ttaattgaag ttgatccagt tttatcacgt
    19441 gttagtgtac atttagcaaa cttcttagaa attcctttcg atgtgtatcc tcaagatgcc
    19501 atcatgccac taccattaga agaagcagat atcgttattg gtgattttcc agtaggctat
    19561 tatccaattg atgaaagaag taaggagttt aagctaggtt ttgaagaagg acatagttat
    19621 tcacattatt tattaataga acaagcaata aatgcattaa aagatgctgg atatgccttt
    19681 ctagtggtac caagtaatat ttttacaggt gaacatgtaa aacagcttga aaaatatatt
    19741 gcaacagaga cagagatgca agcattttta aatttaccac caactttatt taaaaatgaa
    19801 aaagcgcgaa aatctatatt aattttacaa aagaaaaaat cgggtgaaac aaagccagtt
    19861 gaagtattat tggcaaatat tcctgatttc aaaattcctt cacaatttca aggatttatg
    19921 acagagttaa atcagtggat ggacacaaat cgtcctaaaa aataatctgt aatagtatag
    19981 tcataaaact gtatgaatga taaaatgaaa aatgaaatag tataaaaagc aaacgatgga
    20041 ggatcattac ttatgtcaaa attaatcttg gctatcaatg ctggtagttc atcattaaaa
    20101 tttcaattaa ttagaatgcc tgaagaggaa ttagtaacaa aaggtttagt agaaagaatc
    20161 ggattgaagg attcaatttt tacaattgaa gtcaacgggg aaaaagttaa aacagtacaa
    20221 gatatcaagg atcacgttga ggcagttgat atcatgttgg atgcttttaa agcgcacaat
    20281 attattaatg atattaacga tatcgatgga acaggtcacc gtgttgtaca tggtggtgaa
    20341 aaattccctg aatcagtagc tattactgat gaggttgaaa aggaaattga agaattaagc
    20401 gaattagcac cgcttcacaa cccagctaac ttgatgggta ttcgtgcttt ccgtaaatta
    20461 ttaccaaata tccctcatgt ggcaattttt gatacagcat tccatcaaac aatgcctgaa
    20521 aaagcatatc tatatagctt gccatatcat tattataaag attatggcat tcgtaagtat
    20581 ggtttccatg gtacaagcca taaatttgta tcacaaagag cggcagaaat gttagataag
    20641 ccaattgaag atttacgtat tatttcatgt cacattggta atggagcatc aattgcagct
    20701 atcgatggcg gtaaatctat tgatacatca atgggcttca caccacttgc tggtgtaaca
    20761 atgggtactc gttctggtaa tattgaccct gctttaattc catttattat ggaaaaaaca
    20821 ggtaaaactg ctgaacaagt attggaaatc ttaaacaaag aatcaggttt attaggatta
    20881 tcaggtacat ctagtgattt acgtgactta tcagaagaag ctgaatcagg taaagcacgt
    20941 tcacaaatgg ctttagatgt atttgcttct aaaattcaca aatatattgg atcatatgct
    21001 gcgagaatgc atggtgttga tgttatcgta tttacagcag gtatcggtga aaactcagta
    21061 gaaattcgtg cgaaagttct tgaaggctta gaatttatgg gtgtttactg ggatcctaag
    21121 aaaaatgaaa acttactacg tggtaaagaa ggatttatta actatcctca ttcaccagtt
    21181 aaagttgtcg ttattccaac agatgaagaa agtatgattg cccgtgatgt tatgacattc
    21241 ggtggtttaa aataattttt aaataaatat taagcgtcta tctccgctac aattatatgt
    21301 aggaggtaga cgctttttgt attgtttgaa aattaactag tgtaatgtct cttttcacga
    21361 atatcttaaa gaattaagta gtcgaagaag attatgaaaa cattattaag tgagttttat
    21421 ttataaacaa taaaaaacca agtgcagaaa gttgcacttg gttcataatg atcaagtagt
    21481 tatctaatga tcatctatat ttaattttga tatttttcac gtaattgagt tgttgcaact
    21541 tgtggttgga agtctgctgg taactcttca gtacgaacaa ctaacacgtc acatggcgca
    21601 tgacgaacga tagattctga tacagaacca acaataaatc tttccacggc atttaagcct
    21661 gatgtaccac tcataatcaa gtctgcatta atttcatgtg caagcttttt aggaatgata
    21721 gatttaggag agccaaactc tagacgcgtt tctacatctt taacaccagc gttagtagct
    21781 acttctttat aaccatttaa taattcttct gcaaaatgct tagatttttc agtaaattga
    21841 gcatcataaa cttcataaga agaatacgtt cttgaatcaa ttacattcac aattgttaac
    21901 ttcgcatcgt tacgtttagc aacaccaact gctctgttaa atgcccattc cgcttcatgt
    21961 gaaccgtcaa ctgcgattaa aatatttttg taagtaatca tagtaagtgc ctccttgttc
    22021 tttcttaatt atattttacc acaattttcg gaaaagtcag tgtaataacc tttacaattt
    22081 tgtggatttt tgattgcgct ttcaaaatgt tcgcgttaag atgattatgg aggtggatgg
    22141 tatgaaaatt ggtataccaa gggagattaa aaataatgaa aatcgtgttg gtttatcacc
    22201 aagtggtgtg cacgctttag tagaaagtgg gcatactgtt ttagtggaaa caaatgcggg
    22261 ttcaggatca ttctttgaag atgtagatta caaagaagca ggtgctgaga ttgttgctga
    22321 acaagcaaaa gtttgggatg tggatatggt tattaaagtt aaagaaccac ttgaatctga
    22381 atatccatat tttaaagaag ggcttgtatt attcacttat cttcatttag caaatgaaga
    22441 aaaattaaca caagctttga tagatagaaa agtaattagt attgcatatg agactgtgca
    22501 gttaccagac cgatctttac cattgttatc accaatgagt gaggtagcag gaagaatgtc
    22561 agctcaagtt ggcgcagagt tcctacaaaa acttaatggt ggtatgggaa ttctactagg
    22621 tggtgtccca ggagtaccta agggtaaagt aactattatc ggtggtggtc aagcaggaac
    22681 aaatgcagct aaaattgcac taggactagg tgcagatgtt acgattttag atgttaatcc
    22741 aaagcgttta caacaattag atgatttatt cggtggacgt gtacatacaa ttatgtcaaa
    22801 tccgttgaat attgagttgt atgttaaaca aagtgattta gtaataggtg cagttttaat
    22861 tccaggtgct aaagcgccaa gacttgtaac agaagacatg attaaacaaa tgaaaaatgg
    22921 gtcagttatt attgacattg ctattgatca aggcggtatt tttgaaacaa ctgataaaat
    22981 tacgacacat gatgatccta catatattaa gcatggtgtg gttcattatg cagttgcaaa
    23041 tatgccaggt gcagtaccgc gtacttcgac gttagcttta aataatgcta cgctacctta
    23101 tgcgctcatg ctagctaata aagggtatag agaagcattt aaatcaaatc aaccattatc
    23161 attaggttta aatacttaca aaggtcacgt aaccaataaa ggcgttgcag aggcatttga
    23221 aatggaatat aaatctgtag aagaagcatt acaattataa tttgaatttg atgaaaaggg
    23281 aatggattac agcgtactaa agaatgacgt tatcatctcc ttggttatta aaattaaata
    23341 atgataaatt aaagctagaa acaatgcgaa gaaatcatcg aacatgaatg atattgattt
    23401 gattcattac atgttactga tgaacatacc tgcatatgtg tttctagctt gtttgttaca
    23461 tattgcattt agacttggat aaaaatttat gtcttaggcg ctacaatgtt atattggcag
    23521 tgtttgactg aatgagaatg cgcttgtaac aagcttttca tttttatccc actccttatt
    23581 tttcgtaatg tgttaatact tcatatcctt cattagtgac aagtatgtca tcttcaattc
    23641 ttacacctgc aacaccaggt acataaatac ctggttcgat tgtaataacc atgccagctt
    23701 ctaacaaatt agaattagta cttgaaacat cttgatattc atgttcttgt aatcctaggc
    23761 catgacctaa gcgatgaggg aaatattcac cataaccttt ttctgaaata atatttctag
    23821 cgatatgatc gatatctttt aatggtattc caggtttaat tgcttggatt gcagatgttt
    23881 ctgcttctaa tacaatatta taaatttctt gtgcttcttt gctaggttca ccaaatttaa
    23941 tagtacgtgt catatcgcta caataatgct cataaattac acctaaatca aatagtacat
    24001 attcattgct ttttaatctg cgatctcctg gtgtgccatg aggtgatgcg gcatgatctc
    24061 caaataaaac catcgtatca aaactcattt cattgacgcc atattgtttg atagtttgct
    24121 caatatggtt gactacttca cattcagtca caccttcttt taaataagaa acacctattt
    24181 cgatacactt atctgctaac tcagcagctt tacgtatttt gctaatttca tcttcggatt
    24241 taatatttct caattgtttg attgttaaat caacatctcc gaatgaattg acattgaaac
    24301 cagagattaa ttgtttttgg cgtgctactg ttaagtgctc gctttcaatt agtaatttat
    24361 tgattgtttg aggataaagt gaaaaagggt tttcagtgtc taaatatcca acgatttcac
    24421 ctgtgaaagg tgatgctttg acttcttcga cttccatttt tggacaaaat agtacttgtt
    24481 taccatcttt cttaatcaat aatgcaaata atctttcatg gggttcgcta cggtatccag
    24541 taaaataata tacattcaac ggtgttgtaa tccatgctgc atcagcttgt tgattgttca
    24601 attcgtctat tatttttgat atttttgtca ttttataaac actcctcaac ataatattac
    24661 ttccatttta tgccaattat tattagattt caaaatcgag ctaatacatt aactagattt
    24721 tcaattttta aaatttgaga atttgttata attaagtggc aaaagggtac taaatttata
    24781 tagtaattga taggaggatg ctcaaatgaa gttatcattt catggtcaat caacgattta
    24841 tttagaaggg aacaacaaaa aggttatcgt agatccgttt atttcaaata accctaagtg
    24901 tgatttgaat attgaaacag tgcaggtaga ctatatcgtt ttaacgcacg gtcattttga
    24961 tcattttggt gatgttgtgg aactagcaaa gaaaacagga gcaacagtaa ttggaagtgc
    25021 agaaatggct gactatcttt cttcatatca cggtgttgaa aatgttcatg gtatgaatat
    25081 tggtggtaaa gcaaactttg atttcggaag cgttaaattt gttcaagcat ttcatagttc
    25141 aagttttaca catgaaaatg gtattccagt ttatttaggt atgccaatgg gtatcgtgtt
    25201 tgaagttgaa ggaaaaacaa tttatcatac tggtgataca ggtctgttta gtgacatgtc
    25261 acttattgcg aaacgtcatc cagttgatgt atgttttgta ccaattggag ataattttac
    25321 gatgggtatt gatgatgcaa gttatgctat taatgaattt attaaaccaa aaatttctgt
    25381 accgattcat tatgatacat tcccattaat cgaacaagat ccacaacaat ttaaagatgc
    25441 tgttaatgta ggggacgtgc aaattttaaa accaggtgaa tctgttcagt tttaaataga
    25501 atatagattt tacactaaaa aacacctcgc taatctatgt ggaagttatc gtctgcatat
    25561 ttttagtggg tgtttttata ttctactgat tcagttacga cgtggacggg ggacatcagt
    25621 ggttctttat catttctgac ctactctcgt aattttagtt ttatacaatt agacattgat
    25681 tataaattta ctacgatata aaaaatccag aaccacagtc ataatcgttt cttgtattta
    25741 ctcagtcgtt tgagactgca tcatgattga aagattattt gtgattctgg ataaaaattt
    25801 tatttaacga ttaatacagg gatagtcgca cgttttgcta ctttgtggct gacacttcca
    25861 agtacaaatt tctttttgtc ttctgcttta cggttactta aaacaacaat ttcgtattta
    25921 ccgctattag catgttttaa tagttcttct tttgcattac cacgaacaat tatttggtca
    25981 taatcaatac cataatcttc taaagctttg cgtgtttttt ccaatcgctt gcttcgttct
    26041 tcagtaagtt tgtttaaatg aacacctgct ttaattgatg cttgagcatc ttgttcgctg
    26101 attgcgttta aaactgttac gactgtacct tcgccagcta atttagacac ttcttttagt
    26161 gctttttcat tttttaactg agtgtctaca ccaagtaata tatttttata cattagtaca
    26221 tccctcctca cttacattct aataaataat aatggatttt tccatttaaa attaaagatt
    26281 ttgtgatctt tgtgtttgtt gtatattttc aaaaaataaa ataggtgaaa cgatcattat
    26341 ttcatttcac ttcaatgctt tttatccaaa ataaagttat aatgtagtta atactgttcg
    26401 aaaagaagag gtaaagaatg acaaaacatg aacaaatttt agattatatt gaatcgttat
    26461 ctataggctc taaaatttcg gtgcgtaaaa tcgctaaatt tttaaacgta tctgaaggga
    26521 cagcatatcg tgcaattaaa gatgctgata aaatgggcat ggtcgcaacg atagatcgtg
    26581 ttggtacggt tcgaatcgaa aaacgtaatc gtaatgaaat tgaacattta acttttaatg
    26641 aaattgtcaa tattattgat ggccaagtgt taggtggcaa caaaggtatc actaagatgg
    26701 tatctaaatt tgcaattgga gcaatggaac tcaaagatat tttaaagtat attggtccaa
    26761 agacattatt gattgtcgga aatcgtgaag atgtgcagat tgaagcttta aaaagaggta
    26821 cggctatatt aattactggt ggatttaagc catctaataa agttattaat tttgcgaatg
    26881 aacatgattt acctgttcta tcatcaagtt atgatacatt cctagttgca aatattatca
    26941 acaaagcact ttttaatcag aaaatacgaa aagatatctt aatagttcaa gatataatga
    27001 cgccattaga tgatttatct gtgctgtttg atacgatgaa aatagcagat tataagcgta
    27061 tggcgaatcg aactggccat acaagatttc cggtagtcaa tgagtcgtat aaattagtag
    27121 gtattgtaac aagtcgagag atgattaata caaaggacga cgacgaaata gataaagtga
    27181 tgacgcgaaa tcctatttat gtaaatgcta tgagtacagt agcaagttgt gcgcatatga
    27241 tgatttggga aggtattgaa ttgattcctg tcgtttcgag taataaaaag acagtgggtg
    27301 tgattaatag acaagatgtt ttaaagtcta tgcaactttt aggaagacag cctcagatgg
    27361 gtgaaacaat taacgatcaa atcgccaaat atataacaat gaatcaagat ggaattacag
    27421 tagaagtttc accactttta atcaatcatt acggaacagt aagtaaagcg gcatttgtaa
    27481 gtattataga agaaacaata caatatgaaa tgcgtaaatt taaaaaaggt aatgtcatga
    27541 tagagaactt aaatatcgtg tatattaaaa cagtacctat tgaatcacat ataacagtgc
    27601 gctttggcat actagacgtc ggaagaaact tcgccaaaat agaagtgaac atgcatagtc
    27661 aaaacgacaa agtagcgagc gctctcgtga tatgccaaat gtttgacgaa gtataggaac
    27721 ttagcagaga aaataggcat aagatagatt tcgcacaaac gttggggccc caccccaact
    27781 tgcattgttt gtagaaatta ggggtccaat ttctctgtgt tggggcccct gaactaaata
    27841 gtatgaaaat atatatatct aaataattaa aacgggcaaa tagtttaata tggtagtaac
    27901 aatttttata atttagatat tttgatatag actaatgctt aaattaagca acttttctaa
    27961 aaaagggagt atgaaaatga ttagtacttt gaatgaaatt atgaaatgta tcgaagacaa
    28021 cgatacaatt attatacaca gacatgttag accagatcct gatgcatatg gttcacaact
    28081 tggtttaaaa tactatattc agcagaaatt tccgcaaaag caagtatttg cagtgggtga
    28141 agcggaatca tcattaagtt ttattggaga gttagataac attgatgata aaacatatca
    28201 agatgcgctt gtaattgtat gtgatactgc caatgctcca cgaattgacg atgaacgtta
    28261 tagtacaggt cgtaaactta ttaaaataga tcatcatcct gcagttgatc agtatggtga
    28321 tattaattta gttaatacga acgcgtcatc tacaagtgaa atcatttacg atttaatctc
    28381 acattttaat gatgaagcaa ttgttaataa agatatcgcg agcgttttat atcttggtat
    28441 cgtcggtgat actgggcgat tcctttttaa caatacctca gaacatacta tggaaattgc
    28501 tggaaagtta attgggcatg atattgatca taatgcgctt ttaaataaaa tgatggagaa
    28561 ggacccaaaa atgttgccgt ttcaaggtta tgttttacaa catttcgaac ttatggatga
    28621 tggattctgc caagttaaaa taactgaaga tgtattggag caattcggta ttcagccaaa
    28681 tgaagcatct cagtttgtta atacaattgc tgacatcaaa ggcttgaaaa tatgggtatt
    28741 tgcagtcgat gaaggtaatg aaatcagatg tcgattacgt tctaaagggc aattgattat
    28801 taatgatatt gcgcaagatt ttggtggcgg tggtcatccg aatgcgtcag gagtttcagt
    28861 ggacagctgg gatgaatttg agcaacttgc tacagcttta cgcacaaaac ttaactaata
    28921 gaaaggggcc attcaatcat ggtggcatat ttaaatattc atacggctta tgatttgtta
    28981 aattcaagct taaaaataga agatgccgta agacttgctg tgtctgaaaa tgttgatgca
    29041 cttgccataa ctgacaccaa tgtattgtat ggttttccta aattttatga tgcatgtata
    29101 gcaaataaca ttaaaccgat ttttggtatg acaatatatg tgacaaatgg attaaataca
    29161 gtcgaaacag ttgttctagc taaaaataat gatggattaa aagatttgta tcaactatca
    29221 tcggaaataa aaatgaatgc attagaacat gtgtcgtttg aattattaaa acgattttct
    29281 aacaatatga ttatcatttt taaaaaagtc ggtgatcaac atcgtgatat tgtacaagtg
    29341 tttgaaaccc ataatgacac atatatggac caccttagta tttcgattca aggtagaaaa
    29401 catgtttgga ttcaaaatgt ttgttaccaa acacgtcaag atgccgatac gatttctgca
    29461 ttagcagcta ttagagacaa tacaaaatta gacttaattc atgatcaaga agattttggt
    29521 gcacattttt taactgaaaa ggaaattaat caattagata ttaaccaaga atatttaacg
    29581 caggttgatg ttatagctca aaagtgtgat gcagaattaa aatatcatca atctctactt
    29641 cctcaatatg agacacctaa tgatgaatca gctaaaaaat atttgtggcg tgtcttagtt
    29701 acacaattga aaaaattaga acttaattat gacgtctatt tagagcgatt gaaatatgag
    29761 tataaagtta ttactaatat gggttttgaa gattatttct taatagtaag tgatttaatc
    29821 cattatgcga aaacgaatga tgtgatggta ggtcctggtc gtggttcttc agctggctca
    29881 ctggtcagtt atttattggg aattacaacg attgatccta ttaaattcaa tctattattt
    29941 gaacgttttt taaacccaga acgtgtaaca atgcctgata ttgatattga ctttgaagat
    30001 acacgccgag aaagggtcat tcagtacgtc caagaaaaat atggcgagct acatgtatct
    30061 ggaattgtga ctttcggtca tctgcttgca agagcagttg ctagagatgt tggaagaatt
    30121 atggggtttg atgaagttac attaaatgaa atttcaagtt taatcccaca taaattagga
    30181 attacacttg atgaagcata tcaaattgac gattttaaaa agtttgtaca tcgaaaccat
    30241 cgacatgaac gctggttcag tatttgtaaa aagttagaag gtttaccaag acatacatct
    30301 acacatgcgg caggaattat tattaatgac catccattat atgaatatgc ccctttaacg
    30361 aaaggggata caggattatt aacgcaatgg acaatgactg aagccgaacg tattgggtta
    30421 ttaaaaatag attttctagg gttgagaaac ttatcgatta ttcatcaaat cttaacacaa
    30481 gtcaaaaaag atttaggtat taatattgat atcgaaaaga ttccgtttga tgatcaaaaa
    30541 gtgtttgaat tgttgtcgca aggagatacg actggcatat tccaattaga gtctgacggt
    30601 gtaagaagtg tattaaaaaa attaaagccg gaacactttg aagatattgt tgctgtaact
    30661 tctttgtata gaccaggtcc aatggaagaa attccaactt acattacaag aagacatgat
    30721 ccaagcaaag ttcaatattt acatccgcat ttagaaccta tattaaaaaa tacttacggt
    30781 gttattattt atcaagagca aattatgcaa atagcgagca catttgcaaa cttcagttat
    30841 ggtgaagcgg atattttaag aagagcaatg agtaaaaaaa atagagctgt tcttgaaagt
    30901 gagcgtcaac attttataga aggtgcaaag caaaatggtt atcacgaaga cattagtaag
    30961 caaatatttg atttgattct gaaatttgct gattatggtt ttcctagagc acatgctgtc
    31021 agctattcta aaattgcata cattatgagc tttttaaaag tccattatcc aaattatttt
    31081 tacgcaaata ttttaagtaa tgttattgga agtgagaaga aaactgctca aatgatagaa
    31141 gaagcaaaaa aacaaggtat cactatattg ccaccgaaca ttaacgaaag tcattggttt
    31201 tataaacctt cccaagaagg catttattta tcaattggta caattaaagg tgttggttat
    31261 caaagtgtga aagtgattgt tgatgaacgt tatcagaacg gcaaatttaa agatttcttt
    31321 gattttgcta gacgtatacc gaagagagtc aaaacgagaa agttacttga agcactgatt
    31381 ttagtgggag cgtttgatgc ttttggtaaa acacgttcaa cgttgttgca agctattgat
    31441 caagtgttgg atggcgattt aaacattgaa caagatggtt ttttatttga tattttaacg
    31501 ccaaaacaga tgtatgaaga taaagaagaa ttgcctgatg cacttattag tcagtacgaa
    31561 aaagaatatt taggatttta tgtttcgcaa cacccagtag ataaaaagtt tgttgccaaa
    31621 caatatttaa cgatatttaa attgagtaac gcgcagaatt ataaacctat attagtacag
    31681 tttgataaag ttaaacaaat tcgaactaaa aatggtcaaa atatggcatt cgtcacatta
    31741 aatgatggca ttgaaacttt agatggtgtg attttcccta atcagtttaa aaagtacgaa
    31801 gagttgttat cacataatga cttgtttata gttagcggga aatttgacca tagaaagcaa
    31861 caacgtcaac taattataaa tgagattcag acattagcca cttttgaaga acaaaaatta
    31921 gcatttgcca aacaaattat aattagaaat aaatcacaaa tagatatgtt tgaagagatg
    31981 attaaagcta cgaaagagaa tgctaatgat gttgtgttat ccttttatga tgaaacgatt
    32041 aaacaaatga ctactttagg ctatattaat caaaaagata gtatgtttaa taattttata
    32101 caatccttta accctagtga tattaggctt atataacttt tatcaactaa taaattatga
    32161 tatagtaaac tgatggttag atatttttta accatcagtt tgcgtttata ttagtttttt
    32221 atgcttatta tttttatgag tttcacttta cattattgat taatccataa gaataattag
    32281 cacaaaaagc agtatacata aattgagtaa agaattttgt cgatatatcg tgcggaaaaa
    32341 taataacttt ttgatatatt ggtttttgct atccattata ataattaaag gtatatcttt
    32401 agatagtatg gtttaatgta tctttataaa tattatttta ttatcgttta tcgctatttg
    32461 tgttacataa tcaaaaccat aaattcttac tcattcagat ttacccaata tttttacttt
    32521 tataatgtaa tgcgttttat ccaagttatt ttttaaaaat aaatattgaa ttaggggctg
    32581 atttcatgtc attaagagat gaagcattgg aaatgcacaa acgtaatcaa ggtaaattag
    32641 aagttaaacc aaatgtaaaa gttactaata aagaggaatt aagtttagca tactcacctg
    32701 gcgttgctga accgtgtaaa gatatttatg aagataaaag aaaagtatat gattacacaa
    32761 ttaaaggaaa tacagttgca gttattactg atggaacagc ggtattaggt ttaggtaaca
    32821 ttggacctga agcaagtatt cctgtaatgg aaggtaaagc agtattattc aaaagcttcg
    32881 ctggtatcaa tggggtgcct attgcgttaa atacaactga taccgaagaa atcattaaaa
    32941 cagttaagtt gttagaacct aattatggtg gtattaattt agaggatatt tcggcaccac
    33001 gttgttttga aattgaagaa cgattgaaaa aagaaactaa tattccggta ttccatgacg
    33061 atcaacatgg tacagcaatt gtaacattgg caggtttggt aaatgcattg agagttgtta
    33121 acaaagatat tgctaaaata aaagttgtac taaatggtgc tggtgcagca ggaatagcca
    33181 ttgttaaatt actatacgcg tatggtgtaa gaaatatggt tatgtgtgac tcaagaggcg
    33241 caatttttga aggacgttca tatggtatga atcctacgaa agatgttgta gcaaaatgga
    33301 caaataaaga taagattgaa gggtctttag aagaagtcgt aaaagatgca gatgtattta
    33361 tcggggtttc tgtagctaat gcgctgtcac aagatatggt taagagtatg gcagataatc
    33421 caattatatt tgcaatggct aatccaaatc ctgaaataat acctgatgat gccaaagcgg
    33481 caggtgcacg agttgttggt acaggacgtt cagactatcc taaccaaatt aataatgtat
    33541 tagctttccc tggtattttt agaggtgcat tagaggttga agctacacat ataaatgaag
    33601 aaatgaaaaa ggcagctgta gaagcgattg ctgatttaat cgatagttct gaattaaatg
    33661 aagactactg tatcccagga ccgtttgata aacgtgtagc gccatcagtt gctcgtaatg
    33721 ttgctaaagc ggcaatggaa tctggagtag ctaggattga agttgatccg caagatgtgt
    33781 atgataaaac aatgaaactt acagatttac aataaaaata attaacgttt aattaaattg
    33841 cttaaaagtt agcaataaag agtcattaaa atgatgaaaa tgaaagttta tgataaacat
    33901 tcaacagtca aacgaatata aatcaaataa atttaaaccc gtttttaact ggtcaagttc
    33961 agtttaaggc tctaaatggt tagaacagag gttatttgga ggttttccta tgtttaaaga
    34021 tttttttaat cgaacaaaga aaaagaaata tcttacagta caagactcta aaaataatga
    34081 tgtgcctgca ggtattatga ctaagtgtcc aaagtgtaag aaaattatgt acacaaaaga
    34141 attagctgaa aatttaaatg tgtgctttaa ttgtgatcat catattgctt taactgcgta
    34201 taaacgtata gaagcaattt ctgatgaagg atcatttaca gaattcgata agggaatgac
    34261 ctctgcgaat ccattagatt ttccaagtta tttagaaaaa attgaaaagg accaacaaaa
    34321 gacaggtctt aaagaagcag ttgtgactgg tacagcacaa ctagatggta tgaaatttgg
    34381 cgttgctgtc atggattcac gttttagaat gggaagtatg ggatcggtta tcggtgaaaa
    34441 gatatgtcgc atcattgatt actgcactga gaaccgttta ccatttattc ttttctctgc
    34501 aagtggtggt gcacgtatgc aagaaggtat tatttccttg atgcaaatgg gtaaaaccag
    34561 tgtatcttta aaacgtcatt ctgacgctgg actattatat atatcatatt taacacatcc
    34621 aactactggt ggtgtatctg caagttttgc atcagttggt gatataaatt taagtgagcc
    34681 aaaagcgttg ataggttttg caggtcgtcg agttattgaa cagacaataa acgaaaaatt
    34741 gccagatgat ttccaaactg cagaattttt attagagcat ggacaattgg ataaagttgt
    34801 acatcgtaat gatatgcgtc aaacattgtc tgaaattcta aaaatccatc aagaggtgac
    34861 taaataatgt tagattttga aaaaccactt tttgaaattc gaaataaaat tgaatcttta
    34921 aaagaatctc aagataaaaa tgatgtggat ttacaagaag aaattgacat gcttgaagcg
    34981 tcattggaac gagaaactaa aaaaatatat acaaatctaa aaccatggga tcgtgtgcaa
    35041 attgcgcgtt tgcaagaaag acctacgacc ctagattata ttccatatat ctttgattcg
    35101 tttatggaac tacatggtga tcgtaatttt agagatgatc cagcaatgat tggtggtatt
    35161 ggctttttaa atggtcgtgc tgttacagtt attggacaac aacgtggaaa agatacaaaa
    35221 gataatattt atcgaaattt tggtatggcg catccagaag gttatcgaaa agcattacgt
    35281 ttaatgaaac aagctgaaaa attcaatcgt cctatcttta catttataga tacaaaaggt
    35341 gcatatcctg gtaaagctgc tgaagaacgt ggacaaagtg aatctatcgc aacaaatttg
    35401 attgagatgg cttcattaaa agtaccagtt attgcgattg tcattggtga aggtggcagt
    35461 ggaggtgctc taggtattgg tattgccaat aaagtattga tgttagagaa tagtacttac
    35521 tctgttatat ctcctgaagg tgcagcggca ttattatgga aagacagtaa tttggctaaa
    35581 attgcagctg aaacaatgaa aattactgcc catgatatta agcaattagg tattatagat
    35641 gatgtcattt ctgaaccact tggcggtgca cataaagata ttgaacagca agctttagct
    35701 attaaatcag cgtttgttgc acagttagat tcacttgagt cattatcacg tgatgaaatt
    35761 gctaatgatc gctttgaaaa attcagaaat atcggttctt atatagaata atcaacttga
    35821 gcatttttat gttaaatcga tactgggttt taccataaat tgaagtacat taaaacaata
    35881 atttaatatt tagatactga attttaacta agattagtag tcaaaattgt ggctactaat
    35941 ctttttttaa ttaagttaaa ataaaattca atatttaaaa cgtttacatc aattcaatac
    36001 attagttttg atggaatgac atatcaattt gtggtaattt agagttaaag ataaatcagt
    36061 tatagaaagg tatgtcgtca tgaagaaaat tgcagtttta actagtggtg gagattcacc
    36121 tggaatgaat gctgccgtaa gagcagttgt tcgtacagca atttacaatg aaattgaagt
    36181 ttatggtgtg tatcatggtt accaaggatt gttaaatgat gatattcata aacttgaatt
    36241 aggatcagtt ggggatacga ttcagcgtgg aggtacattc ttgtattcag caagatgtcc
    36301 agagtttaag gagcaagaag tacgtaaagt tgcaatcgaa aacttacgta aaagagggat
    36361 tgagggcctt gtagttattg gtggtgacgg tagttatcgc ggtgcacaac gcatcagtga
    36421 ggaatgtaaa gaaattcaaa ctatcggtat tcctggtacg attgacaatg atatcaatgg
    36481 tactgatttt acaattggat ttgacacagc attaaatacg attattggct tagtcgacaa
    36541 aattagagat actgcgtcaa gtcacgcacg aacatttatc attgaagcaa tgggccgtga
    36601 ttgtggagat ctagcattat gggctggatt atcagttggt gctgagacaa ttgtagttcc
    36661 agaagtgaaa acagatatta aagaaatagc tgataaaatt gaacaaggta ttaaacgtgg
    36721 taagaaacac tcaatcgttc ttgtagcaga aggttgtatg actgcgcaag attgtcaaaa
    36781 agaattatca caatacatca atgttgataa tagagtgtct gtgttaggtc acgttcaacg
    36841 tggtggtagc ccaacaggtg cggatagagt tttagcatca cgtttaggtg gatatgcggt
    36901 agacttatta atgcaaggtg aaacagctaa gggtgttgga attaagaaca ataaaattgt
    36961 agcaacatct tttgatgaaa tttttgatgg taaagatcat aaatttgatt atagtctata
    37021 tgaacttgct aacaagttat ctatataaga tttcaggagg aattataaaa tgagaaaaac
    37081 taaaattgta tgtacaattg gaccagcttc agaatcagaa gaaatgattg agaaattaat
    37141 caatgctggt atgaacgttg cacgattaaa cttttcacat ggtagtcatg aagagcataa
    37201 aggtagaatt gatacaattc gtaaagtagc taaaagatta gacaaaattg tagcaatttt
    37261 attagataca aaaggtccag aaattcgtac gcataatatg aaagacggta tcattgaact
    37321 tgaacgtggc aacgaagtta ttgttagcat gaatgaagtt gaaggaacac ctgaaaagtt
    37381 ctcagtaaca tatgaaaact taattaacga tgttcaagta ggttcataca ttttacttga
    37441 tgatggctta attgaattac aagttaaaga tattgaccat gctaaaaaag aagttaaatg
    37501 tgatatttta aactctggtg agcttaaaaa caaaaaaggt gttaacttac ctggcgtaag
    37561 agtaagttta cctggtatta cagaaaaaga tgctgaagat atccgtttcg gtattaaaga
    37621 aaatgttgac ttcattgcag caagtttcgt acgtcgtcct agtgatgttt tagaaattcg
    37681 tgaaatttta gaagaacaaa aagctaacat ttcagtattc cctaaaattg aaaaccaaga
    37741 aggtattgat aatattgcgg aaattcttga agtgtctgat ggtttaatgg ttgcacgtgg
    37801 tgacatgggt gttgaaattc cacctgaaaa agtaccaatg gttcaaaaag atttaatcag
    37861 acaatgtaac aaattaggta aaccagttat tacagctaca caaatgttag attctatgca
    37921 acgtaaccca cgtgctacac gtgcagaagc tagtgacgtt gccaacgcaa tctatgatgg
    37981 tacagatgca gtaatgttat ctggtgaaac tgctgctggt ttatatcctg aagaagctgt
    38041 taaaacaatg agaaatattg ctgtatcagc tgaagcagcc caagattaca aaaagttatt
    38101 gtcagatcgt actaaattag ttgaaacttc attagtgaat gctatcggta tttcggttgc
    38161 acatacagct ttaaacttaa atgttaaagc aattgtagct gctactgaaa gtggttcaac
    38221 ggcacgtact atctccaaat atcgtccaca ttcagacatt attgcggtga ctccaagtga
    38281 agaaactgca cgtcaatgtt caattgtttg gggagttcaa cctgtagtta aaaaaggacg
    38341 taagagtaca gatgcattgt taaacaatgc agttgcaaca gctgttgaaa ctggtagagt
    38401 atctaatggt gatttaatca ttattactgc tggtgtacca actggtgaaa ctggaactac
    38461 taatatgatg aaaatccacc tagttggtga cgaaattgct aatggtcaag gtattggacg
    38521 tggatcagtt gttggtacta cgttagttgc tgaaactgtt aaagatttag aaggtaaaga
    38581 tttatctgac aaagttatcg ttactaactc aatcgatgaa acgtttgtac cttatgtaga
    38641 aaaagcttta ggcttaatta cagaagaaaa tggtattaca tcaccaagtg caattgttgg
    38701 tttagaaaaa ggtattccaa cagttgtagg tgtagaaaaa gctgttaaaa acataagcaa
    38761 taacatgtta gttacgattg atgctgctca aggtaaaatc tttgaaggat atgcaaacgt
    38821 actataattt ataaaaaaac gtctttccat ttatcaacaa tggaaaggcg ttttttggtt
    38881 catctggtat tttatgacgt aattaatagg ttatttgata atgatagtgt atgaatggca
    38941 atctatataa atgtttatat cttttataca tgtacattat caccttcaaa ctttcactca
    39001 tattactttg gaaatttatt ataaaataga agtatggatg tatttctgaa atgatacatt
    39061 attaaataga tgagaaagta aaagttttga gccaagtacg caatttaata ttataagttg
    39121 catataaaac agg
//
LOCUS       DANRDI010000008        39025 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.008, whole genome shotgun sequence.
ACCESSION   DANRDI010000008 DANRDI010000000
VERSION     DANRDI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 39025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.008"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..446
                     /locus_tag="PZ377_000254"
     CDS             <1..446
                     /locus_tag="PZ377_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDM6357147.1"
                     /translation="VAIFSLFRIEVDLTFIAAVLTIVGYSINDTIVTFDRVRENLQKV
                     KVITTTEQIDDIVNRSIRQTMTRSINTVLTVIVVVVAILFFGAPTIFNFTLALFIGLI
                     SGVFSSIFIAVPLWGIMKKRQLKKSPKHKLVVYKEKKSNDEKILV"
     gene            649..2922
                     /gene="recJ"
                     /locus_tag="PZ377_000255"
     CDS             649..2922
                     /gene="recJ"
                     /locus_tag="PZ377_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDM6357148.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            2944..3462
                     /locus_tag="PZ377_000256"
     CDS             2944..3462
                     /locus_tag="PZ377_000256"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDM6357149.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            3890..6079
                     /locus_tag="PZ377_000257"
     CDS             3890..6079
                     /locus_tag="PZ377_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDM6357150.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            6091..6543
                     /locus_tag="PZ377_000258"
     CDS             6091..6543
                     /locus_tag="PZ377_000258"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDM6357151.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            6540..7415
                     /locus_tag="PZ377_000259"
     CDS             6540..7415
                     /locus_tag="PZ377_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDM6357152.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            7876..9138
                     /locus_tag="PZ377_000260"
     CDS             7876..9138
                     /locus_tag="PZ377_000260"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDM6357153.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            9154..10920
                     /gene="aspS"
                     /locus_tag="PZ377_000261"
     CDS             9154..10920
                     /gene="aspS"
                     /locus_tag="PZ377_000261"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDM6357154.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            10998..11188
                     /gene="ssrS"
                     /locus_tag="PZ377_000262"
     ncRNA           10998..11188
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PZ377_000262"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11253..11381
                     /locus_tag="PZ377_000263"
     CDS             11253..11381
                     /locus_tag="PZ377_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357155.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            11381..12154
                     /locus_tag="PZ377_000264"
     CDS             11381..12154
                     /locus_tag="PZ377_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDM6357156.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(12315..13589)
                     /locus_tag="PZ377_000265"
     CDS             complement(12315..13589)
                     /locus_tag="PZ377_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDM6357157.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            13674..14096
                     /locus_tag="PZ377_000266"
     CDS             13674..14096
                     /locus_tag="PZ377_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDM6357158.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            14196..14378
                     /locus_tag="PZ377_000267"
     CDS             14196..14378
                     /locus_tag="PZ377_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDM6357159.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            14418..14564
                     /locus_tag="PZ377_000268"
     CDS             14418..14564
                     /locus_tag="PZ377_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357160.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(14801..15814)
                     /locus_tag="PZ377_000269"
     CDS             complement(14801..15814)
                     /locus_tag="PZ377_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDM6357161.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            16126..17268
                     /locus_tag="PZ377_000270"
     CDS             16126..17268
                     /locus_tag="PZ377_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDM6357162.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            17269..18387
                     /gene="mnmA"
                     /locus_tag="PZ377_000271"
     CDS             17269..18387
                     /gene="mnmA"
                     /locus_tag="PZ377_000271"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDM6357163.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            18849..19517
                     /locus_tag="PZ377_000272"
     CDS             18849..19517
                     /locus_tag="PZ377_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDM6357164.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            19519..21996
                     /locus_tag="PZ377_000273"
     CDS             19519..21996
                     /locus_tag="PZ377_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDM6357165.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            22339..24969
                     /gene="alaS"
                     /locus_tag="PZ377_000274"
     CDS             22339..24969
                     /gene="alaS"
                     /locus_tag="PZ377_000274"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDM6357166.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            25032..25292
                     /locus_tag="PZ377_000275"
     CDS             25032..25292
                     /locus_tag="PZ377_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDM6357167.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            25296..25724
                     /gene="ruvX"
                     /locus_tag="PZ377_000276"
     CDS             25296..25724
                     /gene="ruvX"
                     /locus_tag="PZ377_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDM6357168.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            25739..26047
                     /locus_tag="PZ377_000277"
     CDS             25739..26047
                     /locus_tag="PZ377_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDM6357169.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            26332..26970
                     /locus_tag="PZ377_000278"
     CDS             26332..26970
                     /locus_tag="PZ377_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDM6357170.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            26973..27896
                     /locus_tag="PZ377_000279"
     CDS             26973..27896
                     /locus_tag="PZ377_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDM6357171.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            27908..29176
                     /locus_tag="PZ377_000280"
     CDS             27908..29176
                     /locus_tag="PZ377_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDM6357172.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            29176..29799
                     /gene="udk"
                     /locus_tag="PZ377_000281"
     CDS             29176..29799
                     /gene="udk"
                     /locus_tag="PZ377_000281"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDM6357173.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            29827..30303
                     /gene="greA"
                     /locus_tag="PZ377_000282"
     CDS             29827..30303
                     /gene="greA"
                     /locus_tag="PZ377_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDM6357174.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            30629..31363
                     /gene="pxpB"
                     /locus_tag="PZ377_000283"
     CDS             30629..31363
                     /gene="pxpB"
                     /locus_tag="PZ377_000283"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDM6357175.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            31353..32363
                     /locus_tag="PZ377_000284"
     CDS             31353..32363
                     /locus_tag="PZ377_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDM6357176.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            32365..32814
                     /locus_tag="PZ377_000285"
     CDS             32365..32814
                     /locus_tag="PZ377_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDM6357177.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            32828..34189
                     /locus_tag="PZ377_000286"
     CDS             32828..34189
                     /locus_tag="PZ377_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDM6357178.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            34189..34941
                     /gene="pxpA"
                     /locus_tag="PZ377_000287"
     CDS             34189..34941
                     /gene="pxpA"
                     /locus_tag="PZ377_000287"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDM6357179.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            34954..36183
                     /locus_tag="PZ377_000288"
     CDS             34954..36183
                     /locus_tag="PZ377_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDM6357180.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            36472..37692
                     /locus_tag="PZ377_000289"
     CDS             36472..37692
                     /locus_tag="PZ377_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDM6357181.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKIKSRPT
                     IYEISDEFLNSIIK"
     gene            38106..38858
                     /locus_tag="PZ377_000290"
     CDS             38106..38858
                     /locus_tag="PZ377_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDM6357182.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
BASE COUNT        14189 a         5302 c         7502 g        12032 t
ORIGIN      
        1 tagtagcgat tttcagttta tttagaattg aagtagattt aacatttatc gccgctgtat
       61 taacaattgt cggttattca attaatgata caatcgtaac gtttgaccgt gtacgtgaaa
      121 acttacaaaa ggttaaagtg attacgacaa cagaacaaat tgatgatatc gttaatagat
      181 caattagaca gacaatgaca cgttcaatta atacagtatt aacagttatt gtagtagtag
      241 ttgctatact attcttcggt gctcctacga tattcaactt tactttagca ttatttatcg
      301 gattgatttc tggtgtattc tcttcaatct tcattgccgt tccgctatgg ggaataatga
      361 aaaaacgtca gttgaaaaaa tcgccgaaac acaaattagt tgtatataaa gaaaagaaat
      421 cgaacgatga aaagatttta gtttaaaatg aattaagcgg tatgtgaaac aataaagagt
      481 tcacataccg ctttttactt tatttaaatt taacaaaata tatataaata agttcccgca
      541 ttcattttta aaaaatggta taattaaagt cttgattatt tagataagtg ctttttattt
      601 aaagtaattt tagatataat gtcttttgga aatgaggcgt aaatatatat gattaaaccg
      661 aagtataagt ggaaattaac taaaccagct gaatacataa gtgatgaatt aacatcaaaa
      721 ttaaagttaa cgccaatcgt aaaaaaaata ttagaaagta aatcgattat tgatgaacaa
      781 gcaatagaat ccatcatttc agatacagat atcaatcatg atgcactgca attgagcgat
      841 atgactaaga ctattgaaag aattaaaaga gcgatagcga atgatgagaa aatcctagtt
      901 tatggtgatt acgatgccga cggtgttacc gcaactacaa ttttagtgat aacattacaa
      961 cttttaggtg cacaagtagg atggcatatt cctaaccgct ttaccgaagg atatggacca
     1021 aacgaactag catttagaaa tgctcatgat gaaggcataa cattaatcat aactgttgat
     1081 aatggcatac aaggtcataa tgaaattaaa atggttcaag atttaggagt tgatgtcatt
     1141 gtaactgatc atcatgaaat tggtagtaca ttaccagaag catatgcaat cgtacatcca
     1201 atgcatccat cgtttaatta tccgttccaa caattgtgtg gtgcaggtgt tgcatacaaa
     1261 ttagcacaag cattgattga aaatgtacct gattatttta aagcattagt tgcgataggt
     1321 accattgcgg atttagtatc cttaactgat gaaaatcgat cattagtgaa acaaggatta
     1381 aaagtcttaa atgatcaatg tccgacctct gttaaagcat tgctaaaaga agcgggatac
     1441 aatgacaata ttgatgaaga aacaattggt tttattattg gacctagact taatgctgta
     1501 ggtcgtttag atgacgcatc attagcttgc gaattgttga tgactgacgt cgaagaagaa
     1561 gctgcatttt tagcagaaca agttgaacat tttaaccgtg aaagaaaaga tattgtagca
     1621 actatcacag aagaagcaat ggctatggca gaaacgaaag ttaaaaaggg agatttattt
     1681 ttacttttag caaaagaaaa ttggcatgaa ggtgtcttag gtattgttgc ttcgaaaatt
     1741 gtcgaaacat ttgctttgcc aacattaatt ttaaatattg atagggagca aaatcatgca
     1801 aaaggctccg ccagatcaat tgatcaagtg tcgatgtttg aaatactatc agcacatcaa
     1861 gagttaatcg ctaaatttgg cggccatcat atggctgcag gtatgacgat ggatattgaa
     1921 aatatagaat cgttagctga aggtttaaat aaatggatga aagaattatc taaaacaact
     1981 tcgttagatc ccgtaaagca agtagatgta ttattaacag aaaatgatat aacgattaaa
     2041 aatattcgtg atatgaatcg tttaagaccc ttcggaacag atttttctag acctattttt
     2101 gaaatggatg atttatcagt ttcttcagtt aaggcgatag gtcaacaaaa aaatcatcta
     2161 aaattgacac ttggtgaaag taatatcgcc gcgctatttt ggcaaaatgg acacttagaa
     2221 cctgagttac aagatgaaca gccaattaat atattaggat ctgttcaaat caacgaatgg
     2281 aatggtaatc aatcaccgca aataattatt caagatattg cgatgaatga acagcaaata
     2341 ttagattata gaagtaagcg aaaaagttta ccttttacag aaaatgatga aaatattgtc
     2401 gtgcttattc atcctaaaag tgataaagta aatgcgaatg aatattatta tggtgaagaa
     2461 attaaacaac aaactgataa agtagtatta agagatttac caacgtcaat ggaagacttg
     2521 tctaattcct tgcaacaact gcaattttct caactttata tagttttgca acataatcat
     2581 tcgatttact tcgatggtat acctaatatg gatattttta aaaagtgtta taaagcatta
     2641 ataactaaac aagaaacaaa tatccagaaa gagggtatgt tattgtgtca acatttaagt
     2701 gtgaaaccag atacacttaa attcatgttg aaagttttct tagacttaaa atttgtaaca
     2761 caagaagatg gtttaattcg aatcaatcaa caacctgata aaagatcgat tgattccagc
     2821 aaagtatatc aattaagaca acaacgtatg gatgttgaaa agcaattatt atatcaagat
     2881 ttttcagaaa taaaaaattg gataaagtca caattgtcgt gagcaattta ggaggaaata
     2941 ttaatggatt taaagcaata cgtatcagaa gttcaagatt ggccgaaacc aggtgttagt
     3001 ttcaaggata ttactacaat tatggataat ggtgaagcat atggctatgc aacagataaa
     3061 attgtagaat acgcaaaaga cagagatgtt gatatcgttg taggacctga agcgcgtggc
     3121 tttatcattg gctgtcctgt agcttattca atggggattg gctttgcacc tgttagaaaa
     3181 gaagggaaat tacctcgtga agtcattcgt tatgagtatg acctagaata tggtacaaat
     3241 gttttaacaa tgcacaaaga tgcaattaaa ccaggtcaac gtgtgttaat tacagatgat
     3301 ttattagcta ctggtggtac gattgaagca gcaataaaat tagttgaaaa attaggcggt
     3361 atcgtagtag gtattgcatt tataattgaa ttgaaatatt taaatggtat tgaaaaaatt
     3421 aaagattacg atgttatgag tttaatctca tacgacgaat aataaataat ataattttat
     3481 caaatgaaat ccttcatcaa atgtataaga accaatgact taattaaaaa agttgtttaa
     3541 gttttcttaa catgagatgt taggattttt tatttactga aaatgttaga tgattgagca
     3601 ttatacctta ataacatcgt ttatttattt cataaattgt agtatcatag aactaatatt
     3661 taaaaaatga aacagtagat ttaggtcgaa tttttgtaaa agttttaaaa gtaggaatag
     3721 tatacaaatt aaactcgctc aagtaaaatt aatattacga ttaatgacga caggataaat
     3781 atttatcgtc gacggacgta tgattggtgt gggacaaata ctattcaaca agagtaccta
     3841 aatcattgtt taaggcgaag taataaatat gaatggggtg tatcatataa tgaacaacga
     3901 atatccatat agtgcagacg aagttcttca caaagcaaaa tcatatttgt cagcagatga
     3961 atatgagtat gttttaaaaa gctatcatat tgcttatgaa gcacataaag gtcagttccg
     4021 aaaaaacgga ttaccataca ttatgcatcc tatacaagtt gcaggtattt taacagaaat
     4081 gcgattagac ggaccgacga ttgtcgcagg ttttttgcat gatgtaattg aagatacacc
     4141 gtatacattt gaagatgtaa aagaaatgtt caatgaagaa gttgctcgaa ttgttgatgg
     4201 tgtgacgaag cttaaaaaag taaaataccg ctcaaaagaa gaacaacaag ctgaaaatca
     4261 tcgcaagtta tttattgcga ttgccaaaga tgtacgcgta attttggtga aattagcaga
     4321 cagattacat aatatgcgta ccttgaaagc catgccgcgc gaaaaacaaa ttagaatttc
     4381 tcgagaaaca ttagaaattt atgcaccatt agcacatcgt cttggtatta atacaatcaa
     4441 atgggaacta gaagatacgg ctcttcgtta tattgataat gtgcaatatt ttagaatagt
     4501 caatttaatg aagaagaaac gtagtgaacg tgaagcgtat atcgaaacgg ctattgatag
     4561 aatacgtact gaaatggacc gaatgaatat cgaaggcgat ataaatggta gacctaaaca
     4621 tatttacagt atttatcgga aaatgatgaa gcagaaaaaa caatttgatc aaatttttga
     4681 tttgttggcg atacgtgtta ttgtcaattc tattaatgat tgttatgcga tacttgggtt
     4741 ggtgcatacg ttatggaaac cgatgccagg acgttttaaa gattatattg caatgcctaa
     4801 acaaaatttg tatcagtcat tgcatactac agtagtaggc ccaaatggag acccgctcga
     4861 aatccaaata cgaacgtttg atatgcacga aattgctgag catggtgttg cagcacactg
     4921 ggcttacaaa gaaggtaaaa aagtaagtga aaaagatcaa acttatcaaa ataagttaaa
     4981 ttggttaaaa gaattagctg aagcggatca tacatcgtct gacgctcaag aatttatgga
     5041 aaccttaaaa tatgacttac agagtgacaa agtatacgca tttaccccag cgagtgatgt
     5101 tattgagttg ccatatggtg ctgtgccgat tgattttgct tatgcgattc acagtgaagt
     5161 aggtaataag atgattggtg ccaaggtgaa tggcaaaatt gtaccaattg actatatttt
     5221 acaaacaggc gatattgttg aaatacgtac tagtaaacat tcatatggac caagtcgtga
     5281 ttggttgaaa attgttaaat cgtctagtgc caaaggtaaa attaaaagtt tcttcaaaaa
     5341 acaagatcgt tcatctaata ttgaaaaagg ccgaatgatg gttgaagctg aaataaaaga
     5401 gcaaggattt agagtcgaag atattttgac agagaaaaat attcaggttg ttaatgaaaa
     5461 atataacttt gcaaatgaag atgatttatt cgcagctgta ggatttggcg gcgtgacatc
     5521 cttacagatt gttaataaat taactgaaag acaacgtatt ttagataaac aacgtgcttt
     5581 aaatgaagca caagaagtta cgaaatcatt gcctattaaa gacaacatca ttactgatag
     5641 tggtgtctat gtagaaggtt tagaaaatgt acttatcaag ttgtcaaaat gttgtaatcc
     5701 tataccaggt gatgatattg taggttatat caccaaaggt cacggtatta aagtacatcg
     5761 cactgattgc ccaaatatta agaacgaaac tgaacgacta attaatgttg aatgggtaaa
     5821 atcaaaagac gcaactcaaa aatatcaggt tgatttagag gtaactgcgt atgaccgaaa
     5881 tggcttgttg aatgaagtac tacaagctgt tagctcgaca gccggcaatt taattaaagt
     5941 ttcaggacgt tcagatattg ataaaaatgc aataataaat attagtgtca tggtgaaaaa
     6001 cgtgaatgat gtttatcgtg tggtagaaaa gatcaaacaa cttggtgatg tttatacagt
     6061 aacaagagtt tggaactaga ggtgcaaaat atgaaagtag ttgtacaaag agttaaagaa
     6121 gcatcggtga cgaatgatac attaaataat caaatcaaaa aaggatattg tttattagtc
     6181 ggtatcggtc agaactctac agagcaagat gcagatgtaa ttgcaaagaa aattgctaat
     6241 gcaagattat ttgaagatga caataataaa ttaaacttta atatccaaca aatgaatggt
     6301 gaaatactat cagtttcaca atttactctc tatgcagatg taaaaaaagg taaccgtcca
     6361 ggtttctcaa attctaaaaa tcctgatcaa gcggtaaaaa tttatgagta ttttaatgat
     6421 gcgctacgag cgtatggtct tactgtgaaa acaggtgaat ttggaacaca catgaatgtt
     6481 agcataaata atgatggtcc agtcactatt atttatgaaa gtcaggacgg caaaattcaa
     6541 tgaaaaaaat agaggcatgg ttatctaaaa agggtcttaa aaataaacgt actctaatag
     6601 tagtgattgc ctttgtctta tttatcatct ttttattttt attgctgaat agcaatagtg
     6661 aagatagtgg gaacatcacg ataactgaaa atgctgaatt acgtacaggt ccaaacgctg
     6721 cgtatccagt catatataaa gttgaaaaag gtgaccattt taaaaagatt ggtaaagtag
     6781 gtaaatggat tgaagttgaa gatacatcca gtaatgaaaa aggttggata gctggatggc
     6841 acacaaattt agatattgtc gcggataata cgaaggagaa aaatcctttg caaggtaaaa
     6901 caatagtgct tgatcctggt catggaggta gtgaccaggg tgcttcaagc aatactaaat
     6961 ataaaagttt agaaaaagat tatacgttga aaacagcaaa agaattgcag cgtactttag
     7021 aaaaagaagg cgcaactgtt aagatgacaa gaacagacga tacatatgtt tcactagaaa
     7081 atcgtgatat caaaggcgat gcctatttga gtatacataa tgatgcgtta gaatcatcta
     7141 atgcaaatgg aatgacagtt tattggtatc atgataatca aagagcttta gcagatacgt
     7201 tagacgctac gattcagaag aaaggtctac tttctaatcg cggttcaaga caagaaaatt
     7261 atcaagtgtt aagacaaaca aaagttcctg ctgttttatt agaattaggt tatattagta
     7321 acccaactga tgaaacgatg attaaagatc aattacatag acaaatttta gaacaagcaa
     7381 ttgttgatgg ccttaaaatt tatttttctg cgtagggctt gcaaaaatat gtgaaagtag
     7441 ttatcattga tattgaattt tataactaaa accgttagta ttcttgaaat ggtaaatgaa
     7501 ataggtagca atctaactaa gattgtgtag gaatataatc catagactga aagattatgc
     7561 tgagtagttt atatacattg aacacaagaa gaggtgcttt atgaaaagta aagccgttaa
     7621 acgtacgtta aacgttttga gtgggtttat taaatgcacg cttataaaaa gtaatgatga
     7681 ttacaattag gcatgttttt taaaccaatg agggtggcaa cacggatatt cgtcccttgt
     7741 gcagtttcag gctgtgcaag ggctttttct tttgctttta agattatgat ttatcgtgca
     7801 aagttaagtg gtcgtatata gttttagttt taaaaaggta attaaataaa atagtttgcc
     7861 gagggagatg tcaaaatgat taaaatacct agagggacgc aggatatttt acctgaagat
     7921 tcaaagaaat ggcgttacat tgaaaatcaa ttagatgaat taatgacatt ttataattat
     7981 aaagaaataa gaacaccaat ttttgaaagt acagatcttt ttgcaagagg tgttggtgat
     8041 tcaaccgatg tcgtacaaaa agaaatgtat acatttaaag ataaaggcga tagaagtatt
     8101 acattaagac ctgagggaac agctgcagtt gtgcgttcat atattgaaca taaaatgcaa
     8161 ggtaatccaa accaaccaat taaactttat tacaatggac cgatgtttag atatgaacgt
     8221 aagcaaaaag gacgctatcg tcaatttaat caatttggtg tagaagctat tggtgctgaa
     8281 aatcctagcg tagatgcaga agtattagct atggttatgc atatttatca atcatttgga
     8341 ttaaaacatt taaagcttgt tattaatagt gtaggggata tggcgtctcg aaaagaatat
     8401 aacgaagcgt tagtgaaaca ctttgaacca gtaattcatg aattttgttc agattgtcaa
     8461 tcacgtttgc atacaaatcc gatgcgaatt ttggattgta aagtagaccg tgataaagaa
     8521 gcgattaaga ctgcacctag aatcactgat ttcttaaatg aggaatctaa ggcatattat
     8581 gaacaagtaa aagcttattt agatgattta ggtattccat atattgaaga tcctaactta
     8641 gttcgtggat tggattatta tacacataca gcatttgaat taatgatgga taaccctaac
     8701 tatgatggtg ccattacaac gctttgtggt ggtggccgtt ataatggttt attagaattg
     8761 ctagatggtc caagtgaaac aggtattggt tttgcgctaa gtatagaacg attattgctt
     8821 gcacttgaag aagaaggtat cgaattagat attgaagaaa acttagattt attcattgtt
     8881 acaatgggtg atcaagcaga tcgatatgct gtgaagctat taaatcattt gagacataat
     8941 ggtattaaag cagataaaga ctatttacag cgtaaaatta aaggacaaat gaaacaagca
     9001 gaccgtttag gtgccaagtt tacaatcgtt attggtgatc aagaattaga aaataataaa
     9061 atcgatgtta aaaatatgac aactggtgaa tctgaaacaa ttgaattaga cgcattagtc
     9121 gaatatttta agaagtagag agggcgttaa aatatgagta agagaacaac ttattgtgga
     9181 ttagttactg aggcattttt aggacaagaa attacattaa aaggatgggt taacaatcgt
     9241 cgtgaccttg gtggattgat tttcgttgat ttaagagata gagaaggaat tgtacaagtc
     9301 gtgtttaatc ctgcattttc agaagaggca ttgaaaattg ctgaaacagt acgttctgaa
     9361 tatgttgtag aagttcaagg tacagttacg aagcgtgacc ctgaaacagt taatcctaaa
     9421 attaaaactg gccaagttga agtacaagtt acaaatatta aagtgattaa taaatctgag
     9481 acaccaccat tttctataaa tgaagaaaat gttaacgttg atgaaaatat tcgattaaaa
     9541 taccgttatt tagatttacg tcgtcaagag ttagcgcaaa catttaaaat gagacatcaa
     9601 attacacgtt ctattcgtca atatttggat gatgaagggt tctttgacat cgaaacacca
     9661 gtactaacga agtcaacacc tgagggtgca cgtgactatt tagtaccatc tcgtgttcat
     9721 gatggtgaat tttatgcatt accacaatca ccacaattat ttaagcaatt attgatgatt
     9781 agtggatttg acaaatacta ccaaatcgta aaatgcttcc gtgacgaaga tttacgtgca
     9841 gatcgtcaac ctgaatttac acaagtcgat attgaaatga gttttgtaga ccaagaagat
     9901 gtgatgcaaa tgggtgaaga aatgcttaaa aaagttgtta aagaagttaa aggcgttgaa
     9961 attaatggcg ctttcccacg catgacatat aaagaagcga tgcgtcgcta tggttctgat
    10021 aaaccagata cacgttttga aatggaatta attgacgttt ctcaattagg acgtgatatg
    10081 gactttaaag tatttaaaga tactgttgaa aatgatggtg aaattaaagc aattgtcgct
    10141 aaaggtgcag ctgaacaata tactcgtaaa gatatggatg ctttaacaga atttgtaaac
    10201 atctatggtg ctaaaggatt agcgtgggtt aaagttgtgg aagatggttt gacaggtcca
    10261 attggacgtt tctttgaaac agaaaatgtt gaaacattac ttacattaac tggtgctgaa
    10321 gctggtgact tagtaatgtt tgttgcagac aaaccaaatg tcgttgcaca aagtttaggt
    10381 gcattacgtg tcaaattagc taaagaatta ggtttaatcg atgaaacaaa attaaacttc
    10441 ttatgggtga cagattggcc attattagaa tatgatgaag atgcgaaacg ttacgttgca
    10501 gcacatcatc catttacatc tccaaaagaa gctgatattg ctaagcttgg cactgcgcca
    10561 gaagaagctg aggcaaatgc ttatgacata gtattaaatg gttatgaatt aggtggcggt
    10621 tcaatcagaa ttcatgatgg tgagttacaa gaaaaaatgt tcgaagttct tggatttact
    10681 aaagaacaag cacaagaaca gttcggcttc ttactagatg catttaaata tggtgcacca
    10741 ccacatggcg gtattgcatt aggattagac cgattagtca tgttattgac taatagaaca
    10801 aacttgagag atacaatcgc attccctaaa acagcatctg ctacatgttt attaacgaat
    10861 gcgcctggtg aagtttctga taaacaatta gaagaacttt ctttgcgaat tcgtcactaa
    10921 attttaaaaa atcaataaac tttggtttgt tataatttca ttaattttaa ttatgtggta
    10981 atataacatc ataagatagt catctgagtt gttcgtaagt ttgcttttta atttgggcct
    11041 aacactcttt gatcaaggga gcccaatggg ttttcttgca gcgtacacgc cttatatgaa
    11101 ggacgtacaa aacaagaaac agggcaccca cctgtatata acaggccgaa tgatcaagct
    11161 attataacta cggcaatacg gactctatcg atacgcaaga ctttgtcttg cgtattttta
    11221 tgcgtttctt taagttttta ataaagaatt agttgggttt tatagcgttt aataaatgtt
    11281 tcctcgttga attttgggaa aataatcaaa gtgataatgc taaatataaa attttaagtt
    11341 actatgtact tatgtataaa tcagtgaaag aggtaaaata atgaaacatc aattttcaag
    11401 aaatgaacta gcgattggac aacaaggttt agatttattg aagcaacaaa ctgttgtcat
    11461 acttggcgtt ggaggtgttg gttcatttgc tgctgaagca ttagcaagaa cgaatattgg
    11521 tcatattata ttaatagata aagatgatgt tgatattaca aatgtaaaca gacaattaca
    11581 cgctttaact acaacggtag gacaaagtaa agtttctttg atggaagaac gtattaaatt
    11641 aattaatcca gattgtaaag tcacgccctt acatatgttt tatacagaag agacatatga
    11701 agaaattttc gataactacg atatagatta ttttgtagat gcaagtgata caataatgta
    11761 caaagtacac ttaatgaaag agtgtcttga ccgagggatt aaagtgattt ccagtatggg
    11821 tgctgcaaat aaaacagatc caacacgttt tacaatttca gatatctcta aaacacatac
    11881 cgatccaatg gccaaaatta ttcgtcaaaa attgaaaaaa ctaggaattc gtaaagggat
    11941 tccagttgta ttttcagatg aaagtccaat tgtcataaga gaagatgtaa aagatatagt
    12001 tggagataaa aatgctatca atcgaaaagg acaaatgcca ccttcttcaa atgcctttgt
    12061 gccaagtgtt gttggattaa tttgtgcaag ttatgtggtg aatgacgtat taaaagatat
    12121 tccagttcgt cgcattaaag acaaagggca ataattcatt ttgaaaggga taattttcaa
    12181 cgtaaggcaa gtgtaaccac accataaaac taaaactgac tagttcgcaa gcatagtaga
    12241 atatgcctcg tgtactagtc agttttgatt tgattatagc taaaatatca aaggtaatcg
    12301 atttaatctt atttttacgg cctttgttta agtaagttgt tatatatttc tttgagttgt
    12361 tgttcacttt tagatgtcgt ttttggttcg taataaattt tgttttttag tttatcaggt
    12421 aaatattgct gtgaaacata gccattaaca tattggtgtg gatatttgta accaatagat
    12481 cggcctagat ctttagcacc ttgataatgt ccatctttta aatggtttgg aatttggccc
    12541 acatgaccgt ttctaatatc ggacaatgca ctatcaatgg cactcattgc tgaatttgac
    12601 ttaggtgata agcatagttc gattactgct tggcttagtg gaattctagc ttctggtaaa
    12661 cctagacgtt ctgctgattc aatagcagca agtgttctct gaccagcatt aggtgaggct
    12721 aagcctatat cctcatagct aattacaagt aatcgtcgaa ctattgtagg taaatctcca
    12781 gcttcaatta atcgtgctaa ataatgtaaa gcggcattga cgtcgctacc acggatagat
    12841 ttttggaaag cgctcataac atcgtaatgc atgtcaccat ccttgtcact tacaaatgca
    12901 cctttttgta aacagtcttt agcatcttgc aatgtaacat gtcgataacc gtctttgtca
    12961 ttatctgcgc ttaatacagc taattccaat gcatttaacg cactacgaac atcaccttgg
    13021 ctttgtgtag aaaagtaggt catggcatct tcatcaattt tgggttgata tgttttcaaa
    13081 ccattctcat catcttctat tgcacgagtt aacgcttggc gcacatcttc gtcatttaaa
    13141 ggatataact cgaaaatttg cgctcttgaa cgaatcgctg gattgatagc atgataagga
    13201 tttgaagttg tagcaccgat caagacgatt ttgccatttt ctaaatgagg taataaaaag
    13261 tcttgtttag ctttatctag tcgatgtatt tcatctaata acaagataac ttgaccagac
    13321 attttagctt cttcaacaac aagttgcata tcttttttag tgttagttac agcattcaat
    13381 tgtctgaatt tatattgcgt actgcccgaa attgctttgg caatacttgt tttgcctata
    13441 ccaggtggac cataaaaaat cattgaagtt aatttttttg tatcaaccat tcttctgata
    13501 atgcctcttg gtccaactaa atgttgttgg gaaatgattt catctatatt ttttgggcgc
    13561 attctcgatg ctaatggttc tgtactcaca tttgtcacac ctttcttata ctatcttaac
    13621 tcaaaaaatg ctaaaatggg aatatcaata ttcgaattaa gtgaggttat acaatgaaaa
    13681 tttctactaa agggagatat ggacttacat tgatgatttc tcttgctaaa aaagaggggc
    13741 aaggatgtat atcattaaag tcaattgctg aagaaaataa tttgagtgat ttatatttag
    13801 aacagcttgt aggtccttta agaaatgcgg ggttaattcg aagtgtacgc ggtgctaaag
    13861 gtggatacca attaagagtg ccagcggaag aaatctcagc aggggatatt ataagactgt
    13921 tagaaggtcc aattacattt gttgaaagta ttgaatcaga accacctgcg caaaaacaac
    13981 tatggattcg catgagagat gcagtgagag atgttttaga taatacaaca ttgaaatatt
    14041 tagcggaata tgtagataca agtgaagatt tagacggata catgttttat atttaatcgt
    14101 ataaatacta tactataaca taaaaacttc atattataat gtttagcgaa cctccttagt
    14161 ggtatataaa tatatacatc caaggaggtt tcactatggc agacgaaagt aagttcgatc
    14221 aatttaaagg aaatgttaaa gaaacagtag gtaacgttac tgataacaaa gaattagaaa
    14281 aagaaggtca gcaagataaa gtgattggta aagcaaaaga agttgttgaa aatgctaaaa
    14341 acaaaataac tgatgcaatt gataaactta aaaaataagt tcaattaaac taatcaaata
    14401 taagaggagc gattaatatg agttttatgg ataaagctaa agacgcagta gaaaaattta
    14461 aaaatagcga taatgaacaa gttaaaaatg taaaagataa gataaatgaa tatacaggat
    14521 cgaataacga agagaaaaaa gaaaatgaag ataaagagaa ataaatttct ttaaattttg
    14581 gatatgcttt tcaatatttt gaaagcgccc catcaaataa acacttacac agtgtaaagt
    14641 tgaaggggcg ctttcatatt tgcattagaa ttttaagtga atttgaatca ttactaaaat
    14701 aaatgattga acaataaaga catctttcat tcaaaatgct gttactgtat atttttatta
    14761 atattatgga tacatacttt cacttagttt atttatcaca ttataaagtg tttttttcat
    14821 ttatctgatt tataacattc ttgaaccgac gataagagtc taattgcata tctttatcat
    14881 aaatataact tattgccata atttcgttga cttcaccata agtttccata aaatcttgaa
    14941 ttttttgttg aacagtattt tctgaaccta ttaatgatat tttctgtctt tccatagcca
    15001 tcgcgtattc tctcggcgtt aatatatttt gtagttcatg tgttggcggt tgaacgggct
    15061 gcattctgcc acgagtgata ctaaccatta cttgtgccat agatgaagct aaatattgtg
    15121 cttcgtcatc attttcagca acgattgtat ttaaacatac aataacataa ggttcgtcta
    15181 atacatcaga aggttcaaat aacgttttgt aaatttcgat agcttctttc atttgttgag
    15241 gtgcaaaatg tccagcgaac acatatggaa gccctttgcg agcagctaaa tgtgcagaat
    15301 ctgttgaaga accaagaatg tataaaggca catttttacc tactgctgga taagcacgaa
    15361 catatgcttg ttggtgagca gggccgaaat attgttgtaa taatgaaacc tcttctggaa
    15421 atttataaac accatcatgt tgatctcgtc ttaatgcact tgcggtcatc atatctgttc
    15481 caggtgcacg tcctaatcct aaatcgacac gatttggaaa taatgttgcc atcgtgccaa
    15541 attgttccgc aacgattaat ggagcatgat taggtaacat gatgcctcca gaacctacac
    15601 gtatgtgttt cgtatgttct aacgtatgtt gaattaataa agcagttgct gaacttacta
    15661 ggttgggagc gttatggtgt tcagcaatcc aatatctttc atatgataat tcgtctaaat
    15721 gttgtgcgag attaaccata tcattaattg ctgtttgttc atcttcacct tctcgaatag
    15781 ggacaaggtt taatgctgaa tatttaatcg ccatttttat atcgtccttc cttttttatg
    15841 atataagaag tataccaatc gaataaccag ataaatatta aattgctcat aaccaaaata
    15901 atagtacgtt acattttaca aataaaaaaa tatctgcaaa tactaaaatt tataaaaaag
    15961 acaagaataa agcgataaac ttaaaaaagc ctatcgaaaa gcatgtgtag aaaatggtaa
    16021 aatataatta aaaacattat aaataaagtt aaacagaaaa gtagaaaaat agtatgtaag
    16081 gtgctattat tggaacattg ataaaattgt gaggagtgtc caaacatgga aatttatgca
    16141 gattatgctg cgaccacacc agtaaaacct gaagtagtag atgcgatgat gacgatttac
    16201 aattcgcatt atggtaatcc atcatcaata catgctaagg gaagagatgc gcgtaaatat
    16261 ttagatgagt caagacgtca aattgcacaa ttacttggtg cagatacaca tgaaattata
    16321 tttacgagtg gcgcaacaga atcgaataat acggcaatta aaggcatagt aaaagcaaat
    16381 gaacaattgg gtaatcacat tatcacatcg aagattgaac atcattctgt gttacatgtt
    16441 tttgaacaat tagagcgaga aggttttgat gtaacatatc tagatgttga tgatacaggt
    16501 gctattgatt tagatcaact tgaagaaaca attacagata agacgatttt agtatctatt
    16561 atgtttgtaa ataatgaagt tggcacagtg caacaaattt atgatattca agatattatt
    16621 gctgagacaa atgcatattt ccatgttgat gcggttcaag caattggaca tcttgatgtt
    16681 aagtttgacg aatttgagat cgacgcaatg agtattactg ctcataagtt tggcggacca
    16741 aaaggtgtcg gtgcattact agttaaagac catgttacac tagattatcc tcaacttggc
    16801 ggtgaacaag aattgaaacg ccgtgcaggc actgaaaatc ttgcgcaaat tgttgggatg
    16861 gctaaagcat tacaattagc agaaaagaat cgagacgata acaatattca tttaatgaat
    16921 ttaaaagaac aatttttagt taaattacaa gaaagagcga ttccatttga attaaatggt
    16981 tcaatgacgg atgctacagg ccatattgta aatctatatt ttccgtttgt tgaagttgaa
    17041 acgatgttaa cattgttgga tatggcacaa atctatgtgt cgtctggatc tgcatgtaca
    17101 gcaggctcta cacaaccgtc gcatgtttta gatgcaatgt tcgaagatga ggagcgatca
    17161 aatcattcga ttcgatttag ttttaacgaa ttgactactg aaaatgaaat taatgcaatt
    17221 gtagctgaaa ttcataaaat atattttaaa tttaaggagg agtcataatt gtcaaataaa
    17281 gatatacgtg ttgtcgttgg tatgtcaggc ggtgtagata gttctgtaac agcccacgtc
    17341 ttaaaagaac aaggttatga tgtcattggc atatttatga aaaactggga tgacactgac
    17401 gaaaatggcg tatgtactgc aactgaagat tacaacgatg ttattgaagt gtgtaatcaa
    17461 attggcattc cgtattacgc tgttaatttt gaaaaagaat attgggataa agtctttacg
    17521 tatttcttag atgaatacaa aaaaggtcgt actccaaatc cagacgttat gtgtaataaa
    17581 gaaattaagt ttaaagcctt tttagatcat gcgatgaatt taggtgcaga ttatgtagca
    17641 acaggacatt acgcacgcat acatcgtcat gaagatggtc atgttgaaat gttacgtggt
    17701 gtagataata ataaagatca aacatacttc ttgaatcaat tatctcaaca acaactttca
    17761 aaagtgatgt tcccaattgg cgacatcgaa aagagtgaag tgcgtcgaat tgctgaagaa
    17821 caaggacttg ttactgctaa gaaaaaagat tctacaggca tttgttttat cggcgaaaaa
    17881 aactttaaaa catttttatc acaatattta cctgcacaac cgggtgatat gataacactt
    17941 gatggtaaga aaatgggtaa acatagtggt ttgatgtatt acacaatagg acaaagacat
    18001 ggattaggta taggtggaga tggcgatcct tggtttgttg tcggtaaaaa cctaaaagat
    18061 aatgttttat atgtagaaca aggattccat cacgatgcat tatacagtga ttacttaatt
    18121 gcttcagact attcatttgt aaatccagaa gataatgact tagatcaagg ttttgaatgt
    18181 acagctaaat ttagatatcg ccaaaaagat acgaaagttt ttgtgaaacg tgaaaacgac
    18241 catgcactac gtgttacttt tgctgagcca gtaagagcaa tcacacctgg acaagcagtt
    18301 gttttttatc aaggtgatgt gtgtcttggt ggtgcaacaa ttgacgatgt gttcaaaaat
    18361 gaaggtcaat taaattatgt tgtataaaca atggcaacaa taaattactt atttgaagtt
    18421 tcaacgttga aaatgacgaa agacagtttt tgatgagaat aattcatgag gatagagtct
    18481 gggacatcac aatgtcctag gctctacaat gttatattgg cgggaccaca acatagagaa
    18541 tttcgtaaag aaattctaca ggcaatgcca gttggggata acgaatttaa ttttgttaaa
    18601 atatcatttc tgtcccactc cctatgcatg aatctaatta tgtattctta tttttaagta
    18661 cataatagtg gtggctaatg tggaagaacc attacataat aaaccgttaa tggttcttaa
    18721 gcatttctat tccattcccg ctttttcatg aatgaagatg atattagatt atattttatt
    18781 cgttgttaag tgattcgaga catacaattt atcaagatgt tataattgat gagaaatgag
    18841 gttcgtaaat gatagatcaa caaacaattt atcaatacat acaaaatgga aaaatagaag
    18901 aagcgttaca agcattgttc ggaaatatcg aagaaaatcc tacaattatt gaaaattata
    18961 ttaatgctgg tatcgtactt gctgatgcga atgagattga aaaggcagag cgttttttcc
    19021 aaaaagcttt aacaatagat ccgaagaatg gcgtcgtatt ttttaatcta gcaaatgtat
    19081 attataatca gcaacgttat caagaagcta ttaaattata tcaacaagca ttacaaacag
    19141 agattgaaca agttgattgt aattatatga tcggtatggc gtttaatcag ttagaatcat
    19201 ttaagctggc attgccgtat ttaatgactg ctgcggaact agataaagac aaagatgcag
    19261 aagttcaatt tcaatatggt cttgtattat gtcaattaga aatgtttaat gaagccataa
    19321 ctcaacttaa acatgtatta acgattgata aaaatcatgt tgatgcaaga tacaatttgg
    19381 gcttagcgtt atttatgaaa aatgaagata ttgatgaagc aataactcat tttaaagaag
    19441 ctgtgactat cgaccctaaa cacttattaa gtcagcatgc gctgaaaaca ttcactaaaa
    19501 tgaaagagga ggagtaacat gtcagaccct acactatttg attattcaat gatcaaaggt
    19561 actgttgaag cgatattgtt tcaaaacagt gataattttt atacagtgct taaagtagat
    19621 actattgaaa caaatgaaga ctttgataca atgccaactg ttgtagggtt tcttccgaat
    19681 attgttgaag gcgatgtcta cacatttaaa gggcaagttg ttgatcatcc acgttatggt
    19741 aaacaattaa aagcagagac ttttgaaaaa gaaatgccac aaacgaaaga agcaattata
    19801 agttatttat ctagtgattt gtttaaaggt gtaggtaaaa agacagctca aaatattgtt
    19861 aatacattag gtgacaatgc gataaatgat atattagatg accattcagt gctagaaaaa
    19921 gtttcgggat tatctaaaaa gaaacagaaa caaattgcag aacaaatttc agcaaatcaa
    19981 gaatctgaaa aaataatgat tcgtttacat gatttagggt ttggtcctaa actttcaatg
    20041 gcaatttatc agttttatct cggcgataca ctgactattt tagatcgaaa tccatatcaa
    20101 ttgatttatg atattaaagg tattggtttt aataaagcag atcaacttgc tagaaatatt
    20161 ggtatcgctt ataacgataa tgaacgatta aaagctgcgt tgctctatac gttagaagaa
    20221 gaatgtatta aacagggaca tacatatttg cctatcaatg ttgttataga tttaacggtt
    20281 gatgtattaa attatcaaga tgaagaggtc atcgaacctg aaaaattaga cgaaatgttg
    20341 caatacttaa atgaggaaaa acgactaatt attgacaatg aacaagttgc aattccaagt
    20401 ttgtattatt ctgaaatcaa aagtgttcaa aacttattca gaataaagac gcatacaaat
    20461 aaacttacag aaattgaaca gtcagattta caaatgcata ttggtgaaat agaagatgcg
    20521 aatcaagtga actatgcagc ttcacaaaaa gaagctttgc aaacggctat caattctaag
    20581 gtgatgctat taaccggtgg accgggaaca ggaaaaacga cagtaattaa aggtattgtt
    20641 gaattatatg ctgagattca tggcttatca ttagattatg atgattatgt caatgatgat
    20701 tatccagttg tattagcagc gccaacagga agagcgtcta agagactaca agagtcaact
    20761 ggattagaag caatgacgat tcatcgttta ataggttgga atcaagacac aaagccagag
    20821 gatatattag aaaatgaaat aaacgcacga ctcataatca ttgatgagat gtctatggta
    20881 gatacttggt tgttccacca atttttaagt gcagttcctt tagatgcaca attgatattt
    20941 gtaggtgatg aggaccaatt gccttctgta ggtcctggtc aagtatttaa agatttaatt
    21001 gaatcgaaag ctataccacg agtaaatctg actgaagtat atcgtcaaca agatggttca
    21061 agcattatag aattagcaca tcgaatgaag ttgggtcaga aaattgatat tacacagcgt
    21121 tttcatgata gaagttttat taactgtcag gcaaaccaaa taccaacagt tgttgagaag
    21181 gtagttacta gtgctgttaa taaagggtat actatggctg atattcaagt gcttgcacca
    21241 atgtataaag gaaatgccgg tattaaacgt ttaaatcaag ttttacaaga tattttaaac
    21301 cctaaaaaga aagacacacg agaaattgag tttggtgatg tagtctttag aaaaggtgac
    21361 aaagtattac aacttgttaa taggccaaat gataacatat tcaatggtga cataggagtt
    21421 attgttggta ttttttgggc gaaagaaaat gcccttaata aagatgtact tgttgtggat
    21481 tttgaaggta atgaaattac atttacgaaa caagatatga tggaattaac acatgcttat
    21541 tgtacatcaa tccataaatc acaaggttca gaatttccta ttgttatcat gcctattgtt
    21601 aaacagtatt ttagaatgtt acaaagaccg atattatata ctggtttaac tagagcgaaa
    21661 acgagtttag ttttacttgg tgatccggaa gcatttgata ttggattgaa aacaaatggt
    21721 caagcgcgtt taacacaatt atgtacattg ttgaaaaatt attttaattc agaagatgaa
    21781 gaaatgctgg aaaacacagc aactaatgat acaggagctt cacaaacgac aatagatgat
    21841 caagtagaag cgccaatgtc atcgaatgat agtgaagaag ttatgtctga ttcaacaaaa
    21901 acagatacaa atgtattaac ggaagctaca atgtttaaaa ttgacccaat gattaatatg
    21961 ggagaaatca cgccgtatga ctttatagaa cgttgacaac aagcgtaaat gtaactaaaa
    22021 tagaattaaa ttcgtataaa gatgagtagt taatgatttg tttatcagag atgtactggt
    22081 tggtgaaaag tacaacaaac attagtgaac gctactttat gcacaactgt atagtcgaat
    22141 taaagtgata aatgtgttga attgcattta taactagggt ggtaccgcga taacgttcgt
    22201 cccttttgag gatgggcgtt ttttattttt taataataaa aaatgatatt gatttatact
    22261 agttttgcaa atcgactatc tgtgtcatca atttttatgg aatggtaatt taaaaacagt
    22321 aaataggatg tgtaaatgat gaaaaagtta aaagcgagtg aaattagaca aaaatatcta
    22381 gatttctttg ttgaaaaagg acatatggtt gaaccttctg caccattagt gccaattgat
    22441 gatgatacat tattatggat taattcaggt gtagcaacat taaagaaata ttttgatgga
    22501 cgtgaaacac ctaaaaagcc aagaattgta aactctcaaa aagctattcg tacaaatgat
    22561 attgaaaatg ttggtttcac agcgcgtcac catacattct ttgaaatgtt aggtaacttc
    22621 tcaattggtg attattttaa acaagaagcg attgaatttg cttgggaatt tttaacgagt
    22681 gataaatgga tgggtatgga gccagataaa ttgtacgtta cgattcatcc ggaagatatg
    22741 gaagcataca acatttggca taaagatatt gggcttgaag aaagtcgtat tattcgcatt
    22801 gaaggtaact tctgggatat tggtgaaggg ccttcaggac cgaacactga gattttctat
    22861 gatcgcggag aagcatatgg acaagacgat ccggcagaag aaatgtatcc aggtggagaa
    22921 aatgaacgct atcttgaagt atggaactta gtatttagtg aattcaatca taataaagat
    22981 catagttaca caccattacc taataaaaat attgatactg gcatggggct tgagcgtatg
    23041 gcctcagttt ctcaaaatgt acgtactaac tatgaaacag atttatttat gcctataatg
    23101 aatgaaatcg aaaaagtatc aggtaaacaa tatttagtaa acaacgaaca agatgtggca
    23161 tttaaagtaa ttgctgacca cattcgtacg attgcatttg caatttctga tggtgcatta
    23221 cctgccaatg aaggtagagg gtatgtatta cgtcgattgt tacgtcgtgc cgttcgtttt
    23281 agtcaaacgt taggaatcaa tgagccattt atgtacaaac ttgttgatat tgttgcagac
    23341 attatggaac catattatcc aaatgttaag gaaaaagcag atttcattaa gcgtgttata
    23401 aagtctgaag aagaacgatt ccatgaaaca ttagaagatg gtttagcgat tttaaatgaa
    23461 ttaattaaaa aagctaaagc gacaacaaat gaaattaatg ggaaagatgc atttaaattg
    23521 tatgatacgt atgggttccc aattgaatta actgaagaaa tagcagtgca agcaggattg
    23581 aaagttgata tgacaacatt cgagtcagaa atgcaacaac aacgtgatcg tgcacgtcaa
    23641 gcacgtcaaa attctcaatc aatgcaagtt caaagtgaag tattgaaaaa tattacatct
    23701 gcaagtactt ttgttggtta tgatactgcg acagctcaaa caacactaac acacttgata
    23761 tacaatggtg aagaagtttc acaagttgaa gcgggtgaaa cagtatactt catgttaacg
    23821 gaaacaccat tttatgcaat cagtggtgga caagttgcgg atacaggtat tgtttataat
    23881 gacaattttg aaattgctgt tagtgaagta accaaagcac caaatggtca aaacttgcat
    23941 aaaggagtag tacaatttgg ccaagtaaat gttggcgcta cagtgtctgc tgaagtgaac
    24001 caaaatgatc gacgtgacat tcaaaagaac catagtgcaa cacatttatt acatgcagcg
    24061 ttgaaatcag tactgggtga tcatgttaac caagctggtt cactagtaga agcagatcgt
    24121 ttacgttttg atttctctca ttttggtcca atgactaatg atgaaattga tcaagttgaa
    24181 cgcttagtaa atgaagaaat ttggaaaggt attgacgtta acattcaaga aatggatatt
    24241 gcttcagcta aagaaatggg cgcaatggca ttattcggtg aaaaatatgg tgatgttgtg
    24301 cgtgtagtaa atatggcacc attttcaatt gaattatgtg gtggtattca tgtccgcaat
    24361 acttctgaaa ttggcttatt caaaatagta agtgagtcag gtacaggagc tggtgtgcgt
    24421 cgtattgaag cattaacagg taaagcagct ttcttatatt tagaagatat tcaagagaaa
    24481 tttaatacga tgaaatcaca gctgaaagtg aaatctgatg atcaagtagt cgataagtta
    24541 acacaattac aagatgaaga aaaagcatta ttaaaacaat tagagcaacg tgacaaagaa
    24601 atcacatcac ttaaaatggg taatattgaa gatcaagttg aagaaatcaa tggctataaa
    24661 gtattggtta ctgaagtgga tgtaccaaat gcgaaagcaa ttcgctcgac aatggacgat
    24721 tttaaatcta aactacaaga tacaattatc attcttgcaa gtaatgttga tgataaagta
    24781 tcgatggttg caactgtccc taaatcttta acaaataacg ttaaagccgg tgatcttatc
    24841 aaacaaatgg caccaatcgt tggtggtaaa ggtggcggtc gtccagatat ggctcaaggt
    24901 ggcggtacac aacctgaaaa tatctcaaaa tcattaagct ttattaaaga ttacattaaa
    24961 aatctataat ttaaagccat aatattgtat gatgatagtg agttatattt gtaattaaag
    25021 gagtgtcgca aatggaaaac tttgataaaa caatgaaatt cgactatgaa gaacttccaa
    25081 cgcaagatgt aagagatgtt ttaaataatg tttatcgaac attagatgaa cgaggatata
    25141 atgccgtaaa ccaaattgta ggttatttat tatcaggtga ccctgcgtat attccacgcc
    25201 aaaatgaagc aagaaatcaa attcgtcata ttgatagaga tgttattatg gaagagcttg
    25261 tttcttacta tttaaaagag caaaataaat aatttatgtt acaacataaa attttaggac
    25321 tcgatgtcgg tagtagaacg gtaggaattg caattagtga tataatgggt tggacggcac
    25381 aaggattaga cacactccga atcaatgaag aaaataatga attaggtatt gaccaattag
    25441 tagacattat taaaaaacat aatgtgggta cagtcgtaat aggactacct aaaaacatga
    25501 ataattcaat aggatttcga ggcgaagctt cgttaacata taaagaaaaa ttattagaag
    25561 cttatccttc tattgagatt gtgatgtggg atgaaagatt aagcacaatg gctgctgaac
    25621 gatcattatt agaagcagat gtttcaagac aaaaaagaaa acaagtgatt gataaaatgg
    25681 cagcggtatt tattttacaa ggctatttag attcactaca ttaaaaggag attttactat
    25741 gactgaacat aatcatgatt cacaactaga aattaataac gaagaagaat tattaacttt
    25801 attcgatgaa gagggaaatg aagttttata ccgaaaagtt ttagaatttt atcatcctga
    25861 attcaaaaaa gagtatgtta tcttagctga agaaggtgct caatcagatg aagacgatat
    25921 gattgagctt gtaccaatga tcaatgaacc agatgagtca ggtgacggtg gtaagttagt
    25981 accaatcgaa actgatgaag aatgggacat gattgaagaa gttgtaaata ctgaaatgga
    26041 agaataatta aaaagatttt actcttgcta accttaaaga tggggaggca agagtatttt
    26101 ttaagtcaag caatgtataa ataattaacg cttataattt tattgaaaat atggtaaaat
    26161 atctaaagtt aaattttata tgaatgattc gcttctataa ataaaatagc tttgcgtcta
    26221 acacgaaatc agtcatttaa ttggttggca aaattataat caattgtgaa ggctgattta
    26281 ttttattgtc ataatcatat tgacttattt tacataaagg aagtttaaaa catggatgac
    26341 ctaaataaaa aatatttaat agatttacat caacatcaaa atagttcaat cgaagttttg
    26401 cgtgaatttg ccgaggtaaa tgaagtgcca attgtagatc gtttaacatt agatttaatt
    26461 aagcaattaa ttcgtatgaa taatgttaaa aatattttag aaattggtac agcaatcggc
    26521 tatagttcta tgcaattcgc ttctatatct gatgacattc atgtcacaac gatagagcgt
    26581 aatgaaacga tgattcaata tgctaaacaa aatttagcta cttatcattt tgaaaaccag
    26641 gttcgaatta ttgaaggtaa tgctttagag caatttgaaa atgtaaatga caaagtttat
    26701 gatatgatat ttattgatgc agcaaaagcg caatcaaaga aattttttga aatatataca
    26761 ccacttttaa agcaccaagg tctcgtaatt acagataatg ttttatatca cggttttgta
    26821 tcggatattg ggattgttag atcgagaaat gtaagacaaa tggttaaaaa ggttcaagat
    26881 tataatgagt ggttaataaa gcaaccagga tatacaacga attttttaaa tatagacgat
    26941 ggattagcga tttcaattaa aggagaatga ctatgacaga attacttgtc acacccaagt
    27001 cattaagcca tatggaaaca cttattgatt taggtgcaga tgcttttgta attggcgagc
    27061 aaaagtttgg tttaagactt ccaggggaat tcaatagaca acaaatgaca gaggctgtcg
    27121 cattagctca caaaaacgat aaaaaagtat atgcggcagt gaatggatta tttcataatt
    27181 atcatttaga tgctgtagaa gattatatta attttttaca tgaaatacga gttgacagaa
    27241 ttattttcgg ggatccagca gtagtaatgt atgtgaaggc gcaagaaaat ccaataccac
    27301 ttcattggaa tgcagagacg ttagtaacta atcattttca atgtaattac tggggtaaaa
    27361 gaggcgcatc aagagctgtc ttagcacgtg agctgaattt ggatgaaatt attaatataa
    27421 aagaaaattc aaatgtggaa attgaggtgc aagttcaagg aatgacttgt atgtttcaat
    27481 caaagcgtat gctactaggc aattattata cgttccaaga tcgccaaatg aaaattgaac
    27541 gtcgcaatga tgaacaatct ttattacttt atgatgaaga aagacaaaat aattacccag
    27601 tttacgaaga ttacaatggt acgcacatta tgtcaccaaa cgacatttgt ttaattgaag
    27661 aattagcacc attttttgag gcgggtatag attcatttaa aatcgatggt attctacaaa
    27721 cggaagaata tattaatgtg gtaacggaac agtatcgtca agcgatagat ttgtacaatg
    27781 aagatcctga aatctatgaa gatgagaaat ttatgttgat ggatccaatt gaagaaattc
    27841 aacctgatca tcgaccattt gacgaaggtt tcttatataa acaaacagta tattaaggag
    27901 gttaatcatg aagacaatag aagagattaa atcaactcct aaaacagtta tgaagaaacc
    27961 agaattatta gcacctgctg gaaacttaga aaagttaaaa atagcagtac attatggcgc
    28021 tgatgccgta tttttaggtg gtcaagaata tggattacgt tcaaatgctg ataatttcac
    28081 gatggaagaa atagctgaag gtgttgaatt tgcgaaccgt tacggtgcca aaatttatgt
    28141 tacgacaaat attattgcac atgatgagaa tattgaaggt ctagaatcat atttgcgtaa
    28201 tttggaaaag actggtgcga caggtatcat tgttgcagat cctttaatta tcgaaacatg
    28261 taaagaagtt gcgccaaaac ttgaaattca tttatctact caacaatcac tttctaatta
    28321 caaagctgta gaatattgga aagaagaagg attggatcgt gttgtattag cacgtgagac
    28381 cggcgcgatg gaaatgcgtg aaatgaagga aaaagtagat attgaaatcg aagcatttat
    28441 tcatggtgct atgtgtatcg cctattcagg tagatgtaca ttaagtaatc atatgactgc
    28501 aagggattcc aacagaggcg gttgctgtca aagttgccgt tgggattatg aattattaga
    28561 agttgatgat aatggtgaac ttgatgtttt ttataatcaa ggtgaagtta caccgtttgc
    28621 gatgagtcct aaagatttaa aattaatcga atcaattcct caaatgatgg atattggtgt
    28681 ggactcatta aaaattgaag gacgtatgaa gtcaattcat tatattgcaa cagttgtctc
    28741 agtatatcgt aaagtcattg atgcgtatgc ggcagatcct gacaacttta agattaatcc
    28801 ggaatggtta atagagttag ataaatgtgc aaatagagac actgcaccag cattctttga
    28861 gggaacacct ggttatgaag aacagatgtt tggtcaacaa caatctaaaa aatcaccatt
    28921 tgatttttgt ggtttggtat tagactataa tgaagataca aaaattgcga ctattcaaca
    28981 acgaaataac tttaaaccag gtcaagaaat agaattcttt ggtccagaaa ttgaaacatt
    29041 tacacaagta gtagaagcaa tttatgatga agaaggtaac agcttagacg cggcgcgcca
    29101 tccattacaa atcgttcaaa ttaaagtgga tcgcccgata tatccaaaca acatgatgag
    29161 aaaggaaatt ggctaatgaa agctactaca atcattggca tagctggtgg atctggctca
    29221 ggaaaaacaa ctgtaactaa cgaaattatg aaaaacttag aaggtcatag tgtcgcttta
    29281 cttgctcaag attactatta taaagatcaa aagcacttga ctttcgacga gcgcctagaa
    29341 accaattatg accatccatt tgcattcgat aatgatttat taattgaaaa tcttaaagac
    29401 ttgaaaaatg gtaaagcagt agaagtaccg acatatgatt atgctagtca tacaagaagt
    29461 gacattacca ttgattttaa acctaaagat gttattatcg tagaaggcat tttcgcttta
    29521 gaaaataagg tattacgtga tatgatggat gttaaaatat atgttgatac agatgcagac
    29581 ttgagaatat tacgccgttt aacacgagat actaaagagc gtgggcgttc aatggactct
    29641 gttatcaatc aatatttaag tgttgttaga cctatgcatg accaatttat tgaaccgact
    29701 aagaaatatg ctgatataat tattcctgaa ggtgggagca ataaagttgc aatagatatt
    29761 atgacaacaa aaattcagtc tttagttagc aagcaatagt ataattcagt aaaggaagat
    29821 gacaatatgg aaaatcaaaa gcaatatcca atgactcaag aaggttttga aaaattagag
    29881 cgtgaacttg aagaattaaa aacagttaag cgtcctgaag ttgtagagaa aattaaagtt
    29941 gcacgttcat ttggtgactt atcagagaac tctgagtatg atgcagcaaa agatgaacaa
    30001 ggattcatcg aacaagatat tcaaagaatt gagcatatgt taagaaatgc attaatcatt
    30061 gaagatactg gagataacaa cgttgttaaa attggtaaaa cagtaacgtt tgtagaatta
    30121 ccaggtgatg aagaggaaag ttatcaaatc gttggttcag ctgaatcaga tgcatttaat
    30181 ggtaagattt caaatgaatc accaatggct aaagcgttaa ttggtaaagg tttagatgat
    30241 gaagttcgtg ttccactacc taatggtggc gaaatgaacg taaaaattgt taatatccaa
    30301 taaatgatca aatagattat ttaattgcgc gattgaagtg tgttaatgat acttcggtcg
    30361 tgctttttta tattcaaaat caaaaaatcg gacagatgaa aagtaaaaat tttccatcag
    30421 accgaatgat agcagaacct aaattagtgt aagagttaca atattaacta ctccatttta
    30481 aaaaagcaaa atatttccga gaaaacaaat tgattatcat ttgttattgt atgcgatttg
    30541 aattacttat taaacaactt gtgcttctca atgatacaat gaaaaggtat tcaatactgg
    30601 tttattgcaa tacaaagggg gatttaaagt ggatgtaaga tttattaacg agcagacaat
    30661 tatgatttat tttgaaaata aaatatccga agagacatat cgtaatgtca cagcaatggt
    30721 acggtggata cgagaaaaag aaatattaga aattcaagat attgtccctt cgtatcgagc
    30781 agttctaata tattttgatg aacaagctat aacatcttct aaattaattg aaaatttaga
    30841 acttaacaaa tttaatgaaa aaaatgttca tgctgtgaat caaaccaatc gtattattaa
    30901 aatacctgtt caatatggtg gtacatatgg accagatatt gaagaagtag caaagcataa
    30961 tcgaataact gttgagcaag ttattgaaaa acatacaagt aaaccttatt taatatatat
    31021 gctaggattt atgccaggat ttccatactt aggcggactt gatgaacaac tacatacacc
    31081 tagacgtaat cagccaaggt taaaaattca tgcaggttct gtaggtattg cgaataatca
    31141 aacaggacta tatccatcag attctcctgg cggatggcaa attattggac gcacaccgtt
    31201 aaaagtattt agcagcgagc gtgaaccaat gtcaatgtac gaagcaggag aatggataca
    31261 attttatgca atagatgaac agaaatttat acaaatcgaa agagatatat cagatggcaa
    31321 ctttaatgtt gatgactggg tggtgattga aaatgtcaat taagatttta caaccaggtc
    31381 tcttttcaac ggtacaagat ctaggaagaa taggttatga acatattgga ttttcaggcg
    31441 caggtgccat ggatcaattt agttttaagg ttgcgcagtc attaattaac aatgatggtc
    31501 cagcgattga atatactttg attggtccta ccattcaatt taatacgcaa aatacatttg
    31561 ttataaccgg tggtagtatt aatgcttcgc taaataataa aactatatca atgaattctg
    31621 tcatattagc tgagaaaggt gacattttaa aaataggtgc tataaccaaa ggtgcacgcg
    31681 gttatcttac ttttggtcat tctatcaacg taccttctat tgctgaaagt tatgcaacac
    31741 atacccgaag cagtatagga ggattcaaag gtagaaagtt attagctgat gatgtaataa
    31801 cagtgaaaat aaataatgac tttaaagaaa acattggaaa gacgattcat ttacaggacg
    31861 atttattgcc agaaaataat attatacaca ttcttcaagg acctcaattc gaggcatttt
    31921 ctgaagaggc tagagcgaaa attgtaaatc atccatattt aattactgaa caatcagacc
    31981 gtatgggcta tcgtttggaa ggtgacagcg ttgcaccaat taatcaagca gatatcattt
    32041 ctgaaccggt tgctcttggt agtgttcagg taccaaatga tggtcaacct attattctgc
    32101 ttaatgataa acaaacgata ggtggttata ccaaaattgc aactgtatgc aaatttgatc
    32161 tgccaaagtt agcacagatg aaaccacaag atacaattca atttaaatgg atatcattcc
    32221 aagaagctgt agataagaat cgggaacaaa tgtctctgtt taatgaaatt cttaagagtc
    32281 atcaaaagac tccaattttt gatacatcta gcttgcgaca tacttcgaaa aaattagcaa
    32341 caattttaaa aggggatttg taaaatgaat attgaaaaaa tcgaacaaat aatcaaatta
    32401 gtgaaggaaa atgatgttaa gaaatttaaa tataaaaatt ttgaagatga aatagaaatt
    32461 gacttcactg actcgaatca tttggctgca cacagtaatc aatcaaatca aagtatgaac
    32521 aataatgatt tgacagcttc aaaagcgaat gataactccg atgtttcgac aaatgattat
    32581 catgacatta aatcaccaat gataggtaca ttctttttac aagatagtaa agaattaact
    32641 gaaccaattg tgaatgtcgg tgacaaagtt aacaagggag atattatagg atatgttgaa
    32701 gcgatgaaag tattaaacga ggtaacaaca gatgttgctg gagaaattac tgaaatagta
    32761 gctgatcatg gcacaaatgt tgaatacgac caagttttgg tacgtattaa gtaaaggggg
    32821 taagtaaatg cttcgttgtt taattgcgaa cagaggtgaa attgctgtaa ggattataag
    32881 agcttgcaga gaatatggga ttgaaactgt agcagtttat gcaaaagggg acgagcaaag
    32941 cttgcatgta catttagcag atcaagctat atgtattggg gaagctaatg ctttagatag
    33001 ttatttaaat attgaccgca tcatatctgc tgcacaaatc actggtgcta atgcaattca
    33061 cccaggatat ggctttttat cagaatcaac aaaatttgct caaaccgttg aggaacaagg
    33121 catagcgttt atagggccta ctaaaaaaac gatggaaatg atgggggata aaataacagc
    33181 aagacaaaca gttcatcatg caggtgttcc tgtgattcca ggatcaaatg gtgctgtgaa
    33241 tcatgtgtct gaaattgaga atcttgccaa agacatcggt tatccagttg ttatcaaagc
    33301 tgccagtggc ggtggtggta aaggtatacg tattgtaaag aaagctgaag atttagaaaa
    33361 ggcatttaaa gaagctaaaa gtgaaggaaa aaaatacttt gatgatgatc gtgtttatgt
    33421 tgaggctttt atacctgttg caaaacatgt tgaagttcaa gttatgggag atgggcagga
    33481 taattatgta catttagggg aacgtgattg ttctgtacaa cgtaaaaatc aaaaattaat
    33541 cgaagaatct ccttgtgcag cattaactga agaaagacga caacaaatat gtaacgatgc
    33601 tgttaaagtt gcacgtgcag caaattatag aagtgcaggt acgattgaat ttttagtaac
    33661 tgataccgca cattacttta ttgaaatgaa tgctcgaata caagttgaac atactgttac
    33721 agagatgcga gcagaacgtg atttggttgc agcacaactg tatttattgg aacataatca
    33781 tttacctttt tcacaaagcg atattcaatt taatggtcat gtgatagaag cgagaataaa
    33841 tgccgaaaat cctgaaaaga aatttcaacc aacaccaggt aaagtgacag cgttacattt
    33901 acctcaaggc tttaatgtgc gagtcgattc tttactatat cacggttatc aagtttcacc
    33961 atattatgat tctttagtag ctaaagtaat tgtaaaatct catgatagag cttcagcaat
    34021 tgataagttg aaagtcacct tagatgaaat ggtgatagat ggattttcta caactgccga
    34081 ttttttatat gcagttttaa attatccact atatcgtgat ggagatgcaa aagatgttga
    34141 tattaaattt ttagaaaaac atcaaattgt taaaggggtg gaattataat gcgagttgat
    34201 ttgaattgtg atttaggcga agcatttgga aattattcct ttggtggtga tcatcaaatt
    34261 attccgttaa ttacaagtgc gaatgttgct tgtggttttc acgctggtga tgaaaatgta
    34321 atgaatgaaa cggtaaaact tgccaaagca cataatgttg cagtaggtgc acatcctggt
    34381 ttacctgatt tgaaaggctt tggcagacga aatatagata tctctaacga cgagatttat
    34441 aatttgatga tttatcaatt aggtgcatta caagggtttt gtcgcattca tcaagttaaa
    34501 attaatcatg ttaaaccgca tggtgcattg tatcagatgg gtgcaaaaga cagagaaata
    34561 gcaaacgtta tagcacaagc tgtttatgac tttgatccat cactagtgtt agtaggatta
    34621 gcaaattcat atctaatttc agaagcaaag aatgtcggat taattacagc ttctgaagtg
    34681 tttgctgata gacgatacga agatgatggg cagctcgtta gtagaaaaga aagtgatgct
    34741 gtgattactg atactgacga agcacttaag caggttttaa agatggtgaa ggaaaataaa
    34801 gttatttcaa aaaacaataa ggaagtaacg ttacaagcag atacaatttg tgtgcatggt
    34861 gatggagaac atgcattatt atttgtttcg aaaattagag aaattttaat gaaagaaggc
    34921 attgatattc aatccttata gggggctaaa aaaatgggga gaaatttaaa gcttaaaaaa
    34981 gaaagtgact ttgaattcac aaaaaatcat aaaaggttat tattaggttc tgtatttttg
    35041 atggcaactt ctgcaattgg gccagcattt ttaacgcaaa cagcagtatt tacatcacaa
    35101 ttttttgcaa gtttcgcatt tgccatatta ctgtctatca tcattgacat tggtgcacaa
    35161 attaatatat ggcgcatatt agttgtaact ggtttaagag gtcaagaaat atcaaataaa
    35221 gttgttcctg ggcttggtac tgttatctca atattgattg catttggtgg tctagctttt
    35281 aatattggta atattgctgg tgccggttta ggtttaaatg caatttttgg attagatgta
    35341 aaatggggcg cagctattac tgcaatcttt gcaatattaa tctttgtaag taaaagtggc
    35401 caaaaaatta tggacgttgt ttcaatgatt cttggtattg tgatgatttt agttgtggca
    35461 tatgtgatgt ttgtttctaa tccaccttat ggtgatgctt ttgtgcatac atttgcgcca
    35521 gaacatccaa tgaaattagt cttgcccatc attacgttag ttggtggaac tgtaggtggt
    35581 tatattacct ttgcaggtgc acatcgtata ttagactctg gcattaaagg taagcaatat
    35641 ttaccatttg taaatcaatc agcaattgct ggtattttaa ctacaggtat tatgagaacg
    35701 ttactattcc tagcggtatt aggagttgtt gtaacaggtg tgacactaag ttctgaaaat
    35761 ccaccagcgt cagtttttga acacgcaatt ggaccaattg gaaagaatat ttttggtatt
    35821 gtgttatttg ctgcagctat gtcatcagta attggctcag catacacaag cgcaacattt
    35881 ttaaaaacac ttcataaatc acttaacgaa agaagtaatt taattgtgat tgtgtttatc
    35941 gttatttcaa caatgatttt cttatttatt ggaaaaccaa tcagcctttt aattatagca
    36001 ggcgcgataa atggatggat tttacctatt acgttaggtg caattttgat tgcaagtaaa
    36061 aagaaatcaa ttgttggtga ttataaacac ccaaattgga tgttcatctt tggtatcgtt
    36121 gcagtacttg ttacaatatt gacaggtata ttctcattca aagaagtact tcagttattt
    36181 taaaagacat ttcgactaat agcatcactg taaatgttgt tagtattagg caagatatat
    36241 tttgatttcc ggtgattcat ttataaagca taattagata tattttaagg gagagtggtg
    36301 catgacaaat gtactgctct ttttgttgtg atgaaaaatc tttttctatt gattgagaaa
    36361 atagttattt atatagtttt taaagaaaaa aagtgataat atttagctaa tctaatgaaa
    36421 attgatttat tggacaaaaa atacatttta aatgagtaga ggaggctgca aatgggttac
    36481 agaactttaa aaagtatttt tcatgaacac aatgaaagta aaatgaagga agagtatatt
    36541 aaaagattta attctttagc ttctttcaat actaatatta atatcatacc tatggaaaat
    36601 ggaaaaaaag ttaatgattt ggaatatcct ctattcttta tggtgactaa aaatctatca
    36661 aaaaaacaag aattaatatc aattaatagt agaaaaattg atagagcact taattcttta
    36721 ccatatgcag ctagagaaca atattttaat gatttattaa tcgatgaatt acaaagtact
    36781 aatgaaattg aaaatgtatt tagtactaaa caagagattg cacatgcgtt aaataaccaa
    36841 gcatcagaat ttcttaagtt cagaggcctc gtggatcaat ataaagagat agaacttaat
    36901 aaaaaaatta aagttgataa tgtaagagac attagagcga tttatgataa attagtttca
    36961 aatgaaatta acgaacaaga taagttagat ggagagctat ttcgtaaaaa ttttgtcggt
    37021 gtgcatgatg ggtcaacgaa taaatatata catgttgggt tacaacctga aaccaaaatt
    37081 gttgaattta taggtgaaat gctaacattt ttaaaatatt ttgatgcgcc tcagccgttc
    37141 aaaatcatgg ctagtcatta tctgtttgaa tatatacatc ctttttatga tggcaatggt
    37201 agagttggaa gatttataat tgccaagctc ttgagtgatt attatgataa ttatactgct
    37261 ttgacatttt catatgttat aaatagaaat aaatcgaaat attacaaggc gtttatgact
    37321 gcttcaaatc atttgaattg tggtgatttg acggaattta ttgacacaat gcttgagcta
    37381 ttgatagcag gacaagaaag aatacttgat gagttaatac caaaaatgga tgcgactgaa
    37441 aaattaacgc tttatttaac tagtcattat aaacagatag attatgaatt tttgtatctc
    37501 ctttcaatgg ataaattgtt tggaaataaa agaaatagat taacattaat tgatttagaa
    37561 aacatattgg gagtaggaag agtaaaaatt aataatacaa ttaaaaaata tgataactac
    37621 ttagttaaaa ttaaaagtag accaacaatt tatgagataa gtgatgaatt tctaaattcg
    37681 attataaaat aaatataata tgtgatcagc tatacttata gtatttgtaa gatcgcgtaa
    37741 ttgcttcatt gaaaaaatat atttgttgag agtggtgcat gacaaatgta ctgctctttt
    37801 tgttgtgtat cagtattgtg atgttttaat gagaatatta tatgaatcat tatgaaattt
    37861 aataaaaata aaagaaatga ttatcatttt ttcttatata ctgttaaacg gtttggaatt
    37921 tttaggtata cactgtattg gttgatataa ctcaactaat aattgcgaac agagtatttc
    37981 aaattgaaaa gtattatgag cgtgatacat aatcaaaatt gtaggctcaa gaaccactac
    38041 ataataaacc ataagcggtt ctttatcatt tatgtctcgc tctcaaatgt aaattaataa
    38101 ttgttttggg ggagtttgaa gttaaatatt taacaggatt tattttaata ttattgttag
    38161 aaggaatttt tacaaattca gcgagtgcaa tcgaatattc agacttacat cataaaagta
    38221 agtttgattc aaagcgtcta agtaatgcta agatgtcatt catcaatcca actcagcttg
    38281 aaaataaaaa cacaaacgat agactgttga agcatgattt gttatttcat gacatgttcg
    38341 taaatgatga ttggaaaaag gattttaaag ttgaatttga aaatgaggca ctttcaaaga
    38401 aatttataaa taaggatatc gatatatttg ctggaaatta tggatacgga tgtcatgggg
    38461 gagcaaccaa taaaacgcaa tgtagttatg gtggtgttac tttaagtgac aataataaat
    38521 acgatgatta taagaatata ccttgtaatt tatggattga cggacatcaa acagaaatag
    38581 aactaactgc agtaaaaacg aaaaagaaaa ttgttactat tcaagaatta gaggttcaat
    38641 taagaaatta tttgaatgag aagtataagt tgtacgaaca aggtggcgac attgttaaag
    38701 ggtatgttaa atattataat gatgatgaac aaaatgtaga atatgatttt tataatttaa
    38761 atggtgagta tggtcgtgag gtattaaaaa tgtatgctga taataaaact atcaatagtg
    38821 acaaattgca tttagatatc tatttattca aatcataatt taattaagtt aatcattaat
    38881 aatctttgac agtgaaatat aaagtacctg tcattatgtt gttacaaata aaaacagcag
    38941 taaggatgct aacacttaga aaagtcttac tgctgttttc tattagaatt atctttaatg
    39001 cacaaaggaa ttcgatacga aacaa
//
LOCUS       DANRDI010000009        38154 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.009, whole genome shotgun sequence.
ACCESSION   DANRDI010000009 DANRDI010000000
VERSION     DANRDI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38154
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.009"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            602..796
                     /locus_tag="PZ377_000291"
     CDS             602..796
                     /locus_tag="PZ377_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDM6357183.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(1029..1880)
                     /locus_tag="PZ377_000292"
     CDS             complement(1029..1880)
                     /locus_tag="PZ377_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDM6357184.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            2208..2969
                     /gene="sufC"
                     /locus_tag="PZ377_000293"
     CDS             2208..2969
                     /gene="sufC"
                     /locus_tag="PZ377_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDM6357185.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            3067..4374
                     /gene="sufD"
                     /locus_tag="PZ377_000294"
     CDS             3067..4374
                     /gene="sufD"
                     /locus_tag="PZ377_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDM6357186.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            4480..5730
                     /locus_tag="PZ377_000295"
     CDS             4480..5730
                     /locus_tag="PZ377_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDM6357187.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            5720..6184
                     /locus_tag="PZ377_000296"
     CDS             5720..6184
                     /locus_tag="PZ377_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDM6357188.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            6335..7732
                     /gene="sufB"
                     /locus_tag="PZ377_000297"
     CDS             6335..7732
                     /gene="sufB"
                     /locus_tag="PZ377_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDM6357189.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            7919..8056
                     /locus_tag="PZ377_000298"
     CDS             7919..8056
                     /locus_tag="PZ377_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDM6357190.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(8062..8376)
                     /locus_tag="PZ377_000299"
     CDS             complement(8062..8376)
                     /locus_tag="PZ377_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357191.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            8741..9781
                     /locus_tag="PZ377_000300"
     CDS             8741..9781
                     /locus_tag="PZ377_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDM6357192.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            9795..10862
                     /locus_tag="PZ377_000301"
     CDS             9795..10862
                     /locus_tag="PZ377_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDM6357193.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            10929..11027
                     /locus_tag="PZ377_000302"
     CDS             10929..11027
                     /locus_tag="PZ377_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357194.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            11075..11923
                     /locus_tag="PZ377_000303"
     CDS             11075..11923
                     /locus_tag="PZ377_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDM6357195.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            11936..12763
                     /locus_tag="PZ377_000304"
     CDS             11936..12763
                     /locus_tag="PZ377_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDM6357196.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            12790..14109
                     /locus_tag="PZ377_000305"
     CDS             12790..14109
                     /locus_tag="PZ377_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDM6357197.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            14193..15110
                     /gene="lipA"
                     /locus_tag="PZ377_000306"
     CDS             14193..15110
                     /gene="lipA"
                     /locus_tag="PZ377_000306"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDM6357198.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            15240..15623
                     /locus_tag="PZ377_000307"
     CDS             15240..15623
                     /locus_tag="PZ377_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDM6357199.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(15702..15968)
                     /locus_tag="PZ377_000308"
     CDS             complement(15702..15968)
                     /locus_tag="PZ377_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDM6357200.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            16066..16500
                     /locus_tag="PZ377_000309"
     CDS             16066..16500
                     /locus_tag="PZ377_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDM6357201.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            16500..17279
                     /locus_tag="PZ377_000310"
     CDS             16500..17279
                     /locus_tag="PZ377_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDM6357202.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            17312..18271
                     /locus_tag="PZ377_000311"
     CDS             17312..18271
                     /locus_tag="PZ377_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDM6357203.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            18525..18635
                     /locus_tag="PZ377_000312"
     CDS             18525..18635
                     /locus_tag="PZ377_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357204.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            18683..18835
                     /locus_tag="PZ377_000313"
     CDS             18683..18835
                     /locus_tag="PZ377_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDM6357205.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            18851..20308
                     /gene="dltA"
                     /locus_tag="PZ377_000314"
     CDS             18851..20308
                     /gene="dltA"
                     /locus_tag="PZ377_000314"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDM6357206.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            20305..21519
                     /gene="dltB"
                     /locus_tag="PZ377_000315"
     CDS             20305..21519
                     /gene="dltB"
                     /locus_tag="PZ377_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDM6357207.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            21537..21773
                     /gene="dltC"
                     /locus_tag="PZ377_000316"
     CDS             21537..21773
                     /gene="dltC"
                     /locus_tag="PZ377_000316"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDM6357208.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            21770..22945
                     /gene="dltD"
                     /locus_tag="PZ377_000317"
     CDS             21770..22945
                     /gene="dltD"
                     /locus_tag="PZ377_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDM6357209.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(23208..23450)
                     /locus_tag="PZ377_000318"
     CDS             complement(23208..23450)
                     /locus_tag="PZ377_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDM6357210.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            23551..23874
                     /locus_tag="PZ377_000319"
     CDS             23551..23874
                     /locus_tag="PZ377_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDM6357211.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            complement(23933..24997)
                     /locus_tag="PZ377_000320"
     CDS             complement(23933..24997)
                     /locus_tag="PZ377_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDM6357212.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            25314..25550
                     /locus_tag="PZ377_000321"
     CDS             25314..25550
                     /locus_tag="PZ377_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDM6357213.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            25563..25922
                     /locus_tag="PZ377_000322"
     CDS             25563..25922
                     /locus_tag="PZ377_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDM6357214.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            26376..27584
                     /locus_tag="PZ377_000323"
     CDS             26376..27584
                     /locus_tag="PZ377_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDM6357215.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            27715..29190
                     /locus_tag="PZ377_000324"
     CDS             27715..29190
                     /locus_tag="PZ377_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDM6357216.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            29601..30917
                     /locus_tag="PZ377_000325"
     CDS             29601..30917
                     /locus_tag="PZ377_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDM6357217.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            30936..31310
                     /locus_tag="PZ377_000326"
     CDS             30936..31310
                     /locus_tag="PZ377_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDM6357218.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(31366..32520)
                     /locus_tag="PZ377_000327"
     CDS             complement(31366..32520)
                     /locus_tag="PZ377_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDM6357219.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(32769..33125)
                     /gene="mnhG1"
                     /locus_tag="PZ377_000328"
     CDS             complement(32769..33125)
                     /gene="mnhG1"
                     /locus_tag="PZ377_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDM6357220.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            complement(33103..33396)
                     /gene="mnhF1"
                     /locus_tag="PZ377_000329"
     CDS             complement(33103..33396)
                     /gene="mnhF1"
                     /locus_tag="PZ377_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDM6357221.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            complement(33396..33875)
                     /gene="mnhE1"
                     /locus_tag="PZ377_000330"
     CDS             complement(33396..33875)
                     /gene="mnhE1"
                     /locus_tag="PZ377_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDM6357222.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            complement(33877..35373)
                     /gene="mnhD1"
                     /locus_tag="PZ377_000331"
     CDS             complement(33877..35373)
                     /gene="mnhD1"
                     /locus_tag="PZ377_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDM6357223.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            complement(35366..35707)
                     /gene="mnhC1"
                     /locus_tag="PZ377_000332"
     CDS             complement(35366..35707)
                     /gene="mnhC1"
                     /locus_tag="PZ377_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDM6357224.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            complement(35707..36135)
                     /gene="mnhB1"
                     /locus_tag="PZ377_000333"
     CDS             complement(35707..36135)
                     /gene="mnhB1"
                     /locus_tag="PZ377_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDM6357225.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            complement(36128..>38154)
                     /gene="mnhA1"
                     /locus_tag="PZ377_000334"
     CDS             complement(36128..>38154)
                     /gene="mnhA1"
                     /locus_tag="PZ377_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDM6357226.1"
                     /translation="SDNVIILYLFWELTSFSSFLLISFWRERQASIYGAQKSLIITVF
                     GGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFY
                     IWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWA
                     SLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYAAAFTAAIFHLI
                     NHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPFNGF
                     LSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPE
                     QLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSINHTVIDDVEFHM
                     FHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNRSANVIPNYSEK
                     MTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSA
                     AFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALFLLCFYHL
                     PNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFYQEHVYDLAHGK
                     NMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNHE"
BASE COUNT        13637 a         5651 c         6876 g        11990 t
ORIGIN      
        1 gtcgctacgc cactcatact agttactggg tggttgtttt tatgttatat tataaatgat
       61 caaaccacac cacctattaa tttaggagtg tggttgtttt caagggcaaa aaaagggcat
      121 aattttgaaa caaagggcaa gggtatgaat aataaattat cttgaaaatt ttataaatgc
      181 atatataaca atgttttgat tgcccttgaa cttccttgat aatctggcta attattcctg
      241 ctaaataaat catattaatg gctcagtaac gtgatgttgc tgggcttttt aatttaaaca
      301 ggtatttata tgatatttag gaatgagatg ctattacgga taaagtaaaa tcctatatgc
      361 atgcgtgcta ctcatttatt cttattttta atttcacatg gctagtgact aatacatcat
      421 aaatcattag tgattcttta ttatttcagt cccactccct caagttaatg atgaatctat
      481 ttcgctagtt ataaaatgac gtcaaatcaa tatcaaaaat tatttaagta aaatgtttag
      541 ataatttttc agtgggtaag tattatatat aacatttaat tattccgagg aggcatttat
      601 tatggcagac gaaagtaaat ttgaacaagc aaaaggtaat gttaaagaaa cagtaggtaa
      661 tgttactgat aataaaaatt tagaaaacga aggtaaagaa gataaagctt ctggtaaagc
      721 gaaagaattc gttgaaaatg caaaagaaaa agcaactgat tttattgata aagtaaaagg
      781 taacaaaggc gagtaatttt actttttatt cctaatacac tgcacatgcc acttagtcgg
      841 tataaccatc ggctaggtgg tttttgtatt aaaaaagtgg ataccaaaat ttattaataa
      901 ttattttaat gttagaaaaa aactaaataa aaactcgcta atgatatcca ataatatgta
      961 tacaaaacga gacatatatt gcatatgatt aacgagatac tgaaaatatt ttatcacccc
     1021 taaaatgatt attcattttc agcggtaatt cgacctaaag tcaaacttac aataaaaccg
     1081 atgataaata ctactaatga aacgaaccac atcacgatat tagttggtaa acctggaaat
     1141 actgcaaaga gggagccaac aacaaaacca atgattaatg caaaagtcat tagtttatga
     1201 tgtgttagga aatactggat aattttgctt gaaataatga atccagcaag cacgccaaat
     1261 ccgactgcaa gtaatatagg aagacctgca aagttaagtt taacaacttc agatattgct
     1321 agcatgaccg taccatagac gccaaatact aataacataa atgaccctga aatacctggg
     1381 agtaacatag cactagatgc acacatacct gcaataaaat atttaataat aagactagtt
     1441 gatagagtaa gtgtttctcc agcatgttta tcaccattat tcattaatgt aataacaatt
     1501 aagatagcga taccagctat aaccatcatg taatgtttag ttgtaaatga cgttttatag
     1561 ttagaaattt tcaataaata tggaacgata ccaatgatta atccaccaaa gaaaaacata
     1621 gttggaatat ggtgttggct taataaataa ttaaaaagat tacttagtga tcccattgcc
     1681 agtaacattc caattataat ggggattaaa aatgtaaaac ttggccaaaa acgtcgtgag
     1741 aatatgccgc taattgaagc gataaattga ttgtaaatac ctaacaataa tgcgatagtc
     1801 ccaccgctaa caccaggtac caagtcactc gttcccatag caaaaccttt tagaatatta
     1861 atccatttaa attgttgcat gaataactcc tttcaaacga ttggaataaa atcataaata
     1921 gcatcatacc atattacaaa tgtcctagtg aaatgataac atattttaaa ttcataaaat
     1981 ccattgagaa attatgtgca cttattatca tttatatttt taaagagagc ggattagagt
     2041 tgtactttag agttattaaa aataataaaa agggtttaag ttgtttatat taaggtataa
     2101 gtaagttata attaactgaa cgcattatta caaagtcttt ttgactacaa attaaaatta
     2161 ttataaacta gttaagaaaa ctttatattt tacggaggga atataaaatg gcatcaacat
     2221 tagaaatcaa agacctacat gtgtctattg aggataaaga aatcttaaaa ggtgttaact
     2281 tgacaattaa cactgatgaa atacatgcga ttatgggacc aaacgggaca ggtaaatcaa
     2341 ctttatcatc tgcaattatg ggacacccaa gctatgaagt aactaaagga gaagtacttt
     2401 tagacggtgt aaatatttta gaattagaag ttgatgaaag agcaaaagca ggattattct
     2461 tggcaatgca atatccatca gaaattacag gtgttacaaa tgctgatttc atgcgttcag
     2521 caatcaatgc gaaacgtgaa gaaggacaag aaatcaactt aatgcaattt attaagaaat
     2581 tagataaaaa catggatttt ctagacatag ataaagacat ggcacaacgt tatttaaatg
     2641 aaggtttctc aggtggagag aagaaacgta acgaaatctt acaattaatg atgttagaac
     2701 ctaagtttgc aatcttagat gaaatcgatt cagggttaga catcgatgca ttaaaagttg
     2761 tatctaaagg tattaaccaa atgcgtgggg aaaactttgg tgcattaatg attacacact
     2821 atcaacgatt attaaattac attactcctg ataaagtaca tgtaatgtat gctggtaaag
     2881 tcgttaaatc tggtggtcca gaattagcaa aacgtcttga agaagaagga tatgaatggg
     2941 ttaaagaaga gttcggttca gctgaataat cttattaata cagtatccat gagatgttca
     3001 tctatatatg atgaaaatga acatttatac gaaatagtaa atttcatcaa gtaggaggaa
     3061 aaagttatga caactgatat tttgaacatt tctgaagaac aacttgttga ttattctaaa
     3121 gcccacaatg aaccttcttg gatgacagaa ttacgtaaaa aagctttgaa attaacagaa
     3181 actttagaaa tgccaaaacc tgataaaaca aaattaagaa aatgggattt tgattctttt
     3241 aaacaacacg atgtaaaagg tgatgtttat caatctttat cacaattacc tgagtcagta
     3301 agagaaatta ttgacgtaga tcattctaaa aacttagtaa ttcaacataa taatacgatt
     3361 gcgtacacac aagttgatga taatgcatcg aaagatggcg ttatcgttga aggtttagca
     3421 gacgctctta tgaaccatag tgatttagta caaaagtact ttatgaaaga tgcagtaaca
     3481 gtagatgaac atcgtatcac agcgctacac acggcattag ttaatggtgg cgtatttgtt
     3541 tatgttccta aaaatgtagt tgtagaacat ccagtacaat acgttgtgtt gcacgacgac
     3601 gaaaatgcaa gcttttataa ccatgttatc atcgttactg aagaaagcgc cgaagtcaca
     3661 tatgttgaaa attacttatc aaatgcatct ggtgaaggaa atcaattaaa tattatttct
     3721 gaagtgattg ctggtgcaaa ttcaaatatc acatatggct cagtggacta tatggataaa
     3781 ggctttacag gtcatatcat tcgacgtggt attactgaag cggatgcctc aattaattgg
     3841 gcactaggtt taatgaatga gggtagccaa attattgata atacaacaaa tttatttggt
     3901 gatcgttcaa caagttcact taaatcagta gttgtaggta caggcgaaca aaaaattaat
     3961 ctaacatcta aaatcgtaca atatggtaaa gaaacagatg gttatatcct taaacatggt
     4021 gttatgaaag aacatgcatc gtctgtattt aatggtatcg gctacattaa gcatggtgga
     4081 actaaatcaa ttgctaatca ggaatcacgt gtattaatgt tatctgaaca tgctcgtggt
     4141 gacgcgaatc ctattttatt aattgatgaa gatgatgtac aagctggtca tgctgcatca
     4201 gtaggtcgtg ttgatccaga tcaactttac tatttaatga gtcgtggtat ttctcaaaga
     4261 gaagcggaac gtcttgttat acatggtttc ttagatccag tagtacgtga attacctatc
     4321 gaagacgtta aacgtcaatt gagagaagta attgaacgca aagtttctaa ataatatttt
     4381 gaaaataaaa gtttgtaata gatatagact gtcgatattg gtataagact aatacaacgt
     4441 cagtatttaa atgattagga tttttattta agaaaggtcg tgaatgaagt ggccgaacac
     4501 tcatttgacg ttaatgaagt aatcaaggat tttccgatat tagatcaaaa agtcaatggc
     4561 aaacgtttag catatcttga ttcaacagcg acaagtcaaa cgcctgtgca agtgttaaat
     4621 gttttagaag attactacaa gcgttataat tcaaacgttc atcgtggtgt tcatacatta
     4681 ggatcattgg caactgatgg ttatgaaaat gcccgtgaaa ccgttcgtcg ttttattaat
     4741 gcgaagtatt ttgaagaaat catttttaca cgcggaacaa ctgcgtcgat taaccttgta
     4801 gcacatagct atggtgatgc aaatgttgaa gagggcgatg aaattgttgt cactgaaatg
     4861 gaacatcatg ccaatattgt tccttggcaa cagttagcaa agcgtaaaaa tgcgacattg
     4921 aaatttatac caatgacagc tgacggtgaa ttaaacatcg aggatattaa gcaaacgatt
     4981 aatgataaaa caaagatcgt tgctattgca catatatcta atgtgctcgg tacaattaat
     5041 gatgttaaaa ccattgcaga aatagctcat caacatggtg caattatcag tgttgatggg
     5101 gcgcaagcag caccacatat gaaacttgat atgcaagaaa tgaatgctga tttttatagt
     5161 tttagtggtc ataaaatgct tggaccaaca ggtattggcg tattatttgg taaacgtgag
     5221 ttactacaaa aaatggaacc gattgagttc ggtggcgaca tgattgattt tgtaagtaag
     5281 tatgatgcaa catgggctga tttacctact aaatttgagg cgggtactcc attaattgct
     5341 caagcaattg ggcttgcaga agctattcgc tatttagaac gcataggttt tgatgcaatt
     5401 cataaatatg aacaagaatt aacgatatat gcttatgagc aaatgtctgc aattgaagga
     5461 attgaaattt atggcccgcc aaaggatcgt cgtgcaggtg taataacgtt taatttacaa
     5521 gatgtacatc cacacgatgt tgctacagcc gtagatacag aaggtgtagc ggttagagct
     5581 gggcatcatt gtgcgcaacc gttaatgaaa tggttaaatg tgtcttcaac agctagagcg
     5641 agtttttata tatacaacac gaaagaagac gttgatcagt taataaatgc cttgaaacaa
     5701 acgaaggagt ttttctctta tgaattttaa taatctagat caattatata gatctgtcat
     5761 tatggatcat tataaaaatc ctagaaataa aggtgtatta gataacgggt ctatgacagt
     5821 agatatgaat aacccgacat gcggtgaccg tatacgacta acatttgata tagaagacgg
     5881 cattataaaa gatgctaagt ttgaaggtga aggttgttcg atttcaatgg caagtgcatc
     5941 gatgatgaca caagctgtta aaggtcattc acttggagaa gcaatgcaaa tgagccaaga
     6001 atttacgaaa atgatgcttg gtgaagacta tgtgattaca gaagaaatgg gagatattga
     6061 agcattgcaa ggtgtatctc aattcccagc tcgtattaaa tgtgccacat tagcttggaa
     6121 agcattggaa aaaggtactg ttgctaaaga aggtaaagca gaaggtacga ctgaagaaga
     6181 atagaatgct gttaatcata gataattttg atattagaca tataaaagta taaaaatttt
     6241 tataagatgt catgtcattg ttataatatg gtttacatca tgaattaaaa acttacgcac
     6301 gccgttgtaa atatattttt aaggagtgat tgaaatggct aaaaaagcac ctgatgttgg
     6361 ggattataaa tatggattcc acgacgatga tgtgtccatt ttcagatcag aacgtggttt
     6421 aactgagaat atcgttagag aaatttctaa catgaaaaat gagccggaat ggatgttaga
     6481 tttccgtctt aaatcattaa aattgtttta taaaatgcca atgcctcaat ggggtggcga
     6541 cttatcagaa ttgaatttcg atgacattac ttactatgta aagccttcag aacaagctga
     6601 acgttcatgg gatgaagtgc cagaagaaat taaaagaact ttcgataaat taggaattcc
     6661 tgaagctgaa caaaaatatt tagctggtgt ttctgctcaa tatgaatctg aagttgttta
     6721 ccataatatg gaaaaagaac ttgaagaaaa aggtattatc tttaaagata cagatagtgc
     6781 tttacaagaa aatgaagaat tattcaaaaa atactttgct tctgtagtac ctgcagcaga
     6841 taacaaattt gcggcgttaa actcagcagt atggtcaggt ggttcgttca tttatgtacc
     6901 taaaaatatc aaactagata cgccactaca agcttatttc cgtattaact ctgagaacat
     6961 gggtcaattt gaacgtacat taatcattgc tgatgaaggt gcttctgtac attacgtaga
     7021 aggttgtact gcaccagttt atacaactag ttctttacac tctgctgttg tggaaatcat
     7081 tgtgcataaa gatgcgcacg ttcgttatac tacgattcaa aactgggcga acaatgtata
     7141 caatttagtt acaaaacgta cttttgttta tgaaaacgga aatatggaat gggtagatgg
     7201 taacttaggt tctaagttaa cgatgaaata tccaaactgt gttcttttag gtgaaggtgc
     7261 aaaaggtagt acattatcta ttgcatttgc tggtaaagga caagttcaag atgccggtgc
     7321 taaaatgatt cataaagcac ctaatacatc ttctacaatt gtttctaaat ctatttctaa
     7381 aaatggtggt aaagttattt atcgcggtat tgttcatttt ggacgtaaag caaaaggtgc
     7441 tcgttcaaat attgaatgtg atacattaat cttagataac gaatcaacat cagatacaat
     7501 tccatataac gaagtattca acgatcaaat atcattagaa catgaagcca aggtttcaaa
     7561 agtttctgaa gaacaattat tctatctaat gagtcgtggt atttctgaag aagaagcgac
     7621 agaaatgatt gttatgggat tcatcgaacc atttacaaaa gaacttccaa tggaatacgc
     7681 ggtcgaaatg aaccgtttaa tcaagttcga aatggaaggt agtattggat agctttaaac
     7741 cgcgttgtta agccattctt gacttccgaa aatggctatt gataccattt tgatactgaa
     7801 tataacaaaa agccacatta ctgtggcttt ttttgtttta taactaaatc ggattgatag
     7861 ataagctttg tacttattta tatcagttcg attttttgat tggtgtaaaa aataatcatt
     7921 gatggtggat aaagcgacaa cacaaataca acatgattgt ggcattagag tgctggtctt
     7981 tattaaatta attgaaagct acatcaaata ttctttagat aattcgatat tagttcgatt
     8041 aagattcgtt gtataagtga gttaaaataa gaaaactatt aataatatta agttcactac
     8101 agatgttgct aatggaccat aagttttaaa gacatcttca cttttataac caacaatcgc
     8161 atctaaaaat tgaactaaga tcattgcaat ggatatagtt atcaaaaata tagcactatg
     8221 aatgactaaa gaaaaaatag ctaataaaaa taaaggtaag cttcgactaa gtgcataata
     8281 tgcatttata ttatggctag atgcacatgc ttgaattgaa taacctaaac ttacactggc
     8341 actgattatt gtaaatattg ctaaaacaaa atacatgtta atccttcttt ctatatttgg
     8401 atataaacaa gtacttgtct aaagttattt aaaagataat tagaataaat ttatgagaaa
     8461 ctggttgtta tcattataat ggtttcaaat gattataact atgtcataaa ctgaatttgt
     8521 tgaaattttt cattatgcaa atttattaat aacaaacagc tcgaactata gcatcatttt
     8581 actaatgaat gcattaaagt aactatgact aaaaatgcat attaattatc attattaaga
     8641 ctattatata taatgaattt taactggttt attaaacgag aacgtcggga attaagtaac
     8701 tacaataaaa ataagatatg acaataagga gactacacgc gtgatcattg ccataattat
     8761 attgatattt atttcgtttt tcttttcagg aagcgagacg gcattaacgg ctgccaataa
     8821 aacaaaattt aaaactgaag ctgacaaagg tgataaaaaa gcaaaaggca ttgtaaagtt
     8881 acttgaaaaa ccaagtgagt ttattacaac gattctaatt gggaataatg tcgcgaatat
     8941 tttattacca acacttgtta caattatggc tttacgttgg gggattagcg ttggtattgc
     9001 atcagctgtt ttaacagttg ttatcatttt gatctccgaa gtgattccca agtctgtcgc
     9061 tgcaacattt ccagataaaa taacaaggct tgtatatcca attattaata tttgtgtcat
     9121 tgtgttccgt cctatcacat tacttttaaa taagttgacg gacagtatta atcgaagttt
     9181 atctaagggc caacctcaag aacatcaatt ttcaaaagaa gaatttaaaa caatgttagc
     9241 aattgctgga catgaaggtg ctttaaatga aattgagacg agtaggttgg aaggtgtcat
     9301 taattttgaa aatttaaaag taaaagatgt agatacaaca cctagaatta atgtgacggc
     9361 atttgcttca aatgcgacat acgaagaagt ttatgaaacg gttatgaata agccatacac
     9421 tagatatcca gtgtacgagg gagatattga taacattatt ggggtgtttc attctaaata
     9481 tctgttggct tggagtaata aaaaagaaaa tcaaattaca aactattcag ctaagccatt
     9541 atttgtgaat gaacacaata aagctgaatg ggtattacgt aagatgacta tttctagaaa
     9601 acatttagca attgtgttgg acgaatttgg tggtactgaa gcgatagtgt cacatgaaga
     9661 cttaattgaa gaattattag gtatggaaat tgaagatgag atggataaaa aggaaaaaga
     9721 aaaactttct caacagcaaa ttcaatttca acaacggaaa aatcgcaacg tatctatata
     9781 aggagcgaac agctatgtgg aataagaatc gacttactca aatgttaagt attgaatatc
     9841 caattataca agcaggtatg gcaggaagta cgacaccgaa attagttgca tcagtaagta
     9901 acagtggtgg gttaggcaca ataggcgcag gttactttaa tacgcagcaa ttggaagatg
     9961 aaatagatta tgtacgccaa ttaacgtcaa attcttttgg cgtaaatgtc tttgtaccaa
    10021 gtcaacaatc atataccagt agtcaaattg aaaatatgaa tgcatggtta aaaccttatc
    10081 gacgcgcatt acatttagaa gagccggttg taaaaattac cgaagaacaa caatttaagt
    10141 gtcatattga tacgataatt aaaaagcaag tgcctgtatg ttgttttact tttggaattc
    10201 caagcgaaca gattataagc aggttgaaag cagcgaatgt caaacttata ggtacagcaa
    10261 caagtgttga tgaagctatt gcgaatgaaa aagcgggtat ggatgctatc gttgctcaag
    10321 gtagtgaagc aggtggacat cgtggttcat ttttaaaacc taaaaatcaa ttacctatgg
    10381 ttggaacaat atctttagtg ccacaaattg tagatgtcgt ttcaattccg gtcattgccg
    10441 ctggtggaat tatggatggt agaggagttt tggcaagtat tgtcttaggt gcagaagggg
    10501 tacaaatggg caccgcattt ttaacatcac aagacagtaa tgcatcagaa ctactgcgag
    10561 atgcaattat aaatagtaaa gaaacagata cagtcattac aaaagcgttt agtggaaagc
    10621 ttgcacgcgg tatcaacaat aggtttatcg aagaaatgtc ccaatacgaa ggcgatatcc
    10681 cagattatcc aatacaaaat gagctaacaa gtagcataag aaaagccgca gcaaacatcg
    10741 gcgacaaaga gttaatacat atgtggagtg gacaaagccc gcgactagca acaacgcatc
    10801 ccgccaacac catcatgtcc aatataatca atcaaattaa tcaaatcatg caatataaat
    10861 aatcgaccgc aatccacaaa agcacaagca cccccaaaca ttattttagt gcttgccatt
    10921 tttgtggatt gcgtttctat tttacaaatt taatcaaacg aaaacatcaa gctgaagatc
    10981 gccgaaagat tttaatcaag caaaaacatc aaactaaagt tcgctgaaat gattatgata
    11041 aaagttatat ggtatgatga cattggtgat atatatgata aacatcggat taacaggttg
    11101 gggtgatcac tattcattat atgaagattt agaacgccaa accgataaac ttaaaacata
    11161 tgctggacat tttccggttg tcgaattaga tgcgacatac tatgcgatac aaccggaaag
    11221 aaatatattg aaatggataa aagaaacgcc tgatacattt gaatttgtgg tcaaaattca
    11281 tcaagcactc acattgcatg cagactacaa aacatttgca gatacaaggc aagaactatt
    11341 tgatcaattt aagaatatgt tagagccctt acatacacag aaaaaattag caatggtatt
    11401 ggttcaattt ccgccatggt ttgactgcaa tgcacaaaat atcaaatata ttttgtatgt
    11461 aagacagcaa ttacaagcat ttccaatgtg tgtagaattt aggcatcaat catggtttag
    11521 tgatgcattt aaagaacaaa cattggcatt tttaacagaa catcaaatca ttcatgcagt
    11581 agttgatgaa ccacaagtga aagatggcag tgtaccttta gtcaatcgaa tcacaaatga
    11641 aattgcgttt gtacgttatc atggacgtaa tcattacggt tggactaaga aagatatgtc
    11701 agatcaagaa tggcgcgatg tacgctattt atatgattat aatgagcaag aattaataga
    11761 cttggcacaa aaggcacaaa tattagcaca aaaagctaag aaagtttacg tcatatttaa
    11821 caataattct ggtggtcatg cagcaaataa tgccaaaaca tatcagcgat tattgaatat
    11881 agaatatgaa gggttagcac cacaacaatt aaaattattt taagaggcga cgactatgtt
    11941 attaacaatt acattattag ttttaatcgg aggtttgtca gcgattatag ggtctatcgt
    12001 aggcattgga ggcggtatta ttatcgttcc aacaatggtt tacctcggtg ttgaacatgg
    12061 attactacat aatattacaa cacaagtagc gatagggacg tcttcagtca ttctaattgt
    12121 gacaggactt tcttcatcac ttggatattt aaaaacaaaa caagttgata ttaaaaatgg
    12181 ttccatcttt ttatttggac tattaccagg ttcattgctt gggtccttca ttagtagata
    12241 tttaacattt gagtcattta atttatattt tggtatcttt ttaattttcg tagccatttt
    12301 attaatggta agaaataaga ttaaaccgtt taaaattttc gataaaccca agtatgaaaa
    12361 gacttatgta gacgctaaag gtaaaacata tcattatagt gttccaccat tgtttgcttt
    12421 tattacaacg tttttaattg gtatattgac aggtttattt ggtattggag gtggcgcact
    12481 aatgacgcca ctaatgctta ttgtatttag atttccacct catgtagctg ttggaacaag
    12541 tatgatgatg attttctttt caagtgtcat gagttctata gggcacattg ctcaaggtca
    12601 cgtagcttgg ggttatgcaa tcattttaat tatttctagt tattttggtg cgaaaatcgg
    12661 tgtcaaagtg aatcaatcaa ttaagtcaga tacggtagta acattattga gaacagtaat
    12721 gttgttaatg ggtatatatt taattattcg tgcgttgatt taatacaact ttaaaaggag
    12781 gacgtcaatt tgaggcttac aatttatcat acgaacgata ttcatagtca tttacatgaa
    12841 tacgaacgca ttaaagcata tatggcagaa catcggccac gacttaatca tccttcttta
    12901 tatgttgatc taggtgatca tgtagattta tccgcaccta taactgaagc aactttaggt
    12961 aaaaagaatg tggcattact aaatgaagca aaatgtgatg ttgcaacaat cggtaataat
    13021 gaagggatga ccatttcaca cgaagcttta aatcaccttt acgacgaagc aaaatttata
    13081 gtgacatgta gcaatgttat agatgaatca ggtcatttac caaataatat cgtttcttct
    13141 tatattaagg acatagacgg tgtgaaaata ctattcgttg cagcgacagc accttttacc
    13201 ccattttatc gtgcactaaa ttggattgtt accgatccgc ttgaatctat aaaagaagaa
    13261 attgaacttc aacgaggtaa atttgatgta ttaatcgtgc taagtcattg tggcattttc
    13321 ttcgatgaaa cattatgcca agaattgcct gaaattgatg tcatttttgg tagtcatacg
    13381 catcattatt ttgaacatgg tgaaatcaat aatggtgtac tgatggcggc agctggaaag
    13441 tatggtaatt atcttggaga ggttaattta acttttgagg cacataaagt agtacataaa
    13501 actgcaaaga ttattccttt agaaacatta cctgaagttg aaacttcatt tgaagaagaa
    13561 ggaaaaacgt taatgtccaa ttcagtaatt caacatccag tagtgcttaa gcgtagtatg
    13621 aatcacataa ctgaagctgc atacttatta gctcaaagtg tttgtgagta tacacatgca
    13681 caatgtgcca tcatcaatgc tggcttactc gttaaagata ttgtaaaaga tgaagtgaca
    13741 gaatatgaca ttcatcaaat gttaccgcat ccgattaata tggtaagggt tagacttttt
    13801 ggtgtgaaat taaaagagat tatagctaaa agtaataaac aagaatatat gtatgaacat
    13861 gcacaaggtt tgggtttcag agggaatata tttggaggat atattcttta taatttaggg
    13921 tacattcatt ctacagggcg ttactatctg aatggagaag aaatcgaaga cgacaaagaa
    13981 tatgtactag gtacgataga tatgtatacg ttcggtcgtt atttcccaac attgaaagaa
    14041 ttaccaaaag agtatttaat gccagagttt ttaagagata tatttaaaga aaaattattg
    14101 gaatattaaa aagtaagatt attggatttt catttgtcat gaatttcgat ataatgttta
    14161 aagatacact taacaggagg gtatgtgttg ttatggcgac aaaaaacgag gaaatattac
    14221 gtaaaccgga ttggttgaaa ataaaattaa ataccaacga aaactataca ggacttaaga
    14281 agatgatgag ggaaaaaaat cttaatactg tatgtgaaga agctaaatgt cctaatatac
    14341 atgaatgttg gggtgcacgt cgtacagcga catttatgat tttaggtgcc gtatgtacaa
    14401 gagcttgtcg tttttgtgcg gttaagacag gtttacctaa tgaacttgat ttaaatgagc
    14461 ctgaacgtgt agctgaatca gttgaattaa tgaatttgaa acacgttgtt atcactgctg
    14521 ttgcgcgtga tgatttaaga gatgctggtt caaatgttta tgctgagaca gtacgtaaag
    14581 ttagagaaag aaatccattt acaacgattg aaattttacc atcagatatg ggcggggact
    14641 atgatgcgtt agaaacatta atggcgtcaa gacctgacat tttaaaccat aatattgaaa
    14701 ctgttcgtcg cttaacaccg agagttcgtg cgcgtgcgac ttacgacaga acattagagt
    14761 ttttacgtcg ttcaaaagaa ttacaaccgg atatcccaac taaatcaagt attatggttg
    14821 gattaggtga aactatagaa gaaatttatg aaacgatgga tgatttacgt gcgaatgatg
    14881 tagatatttt aacgattggt caatatttac aaccttcacg taaacattta aaggttcaaa
    14941 aatattacac gcctttagag tttggtaaat taagaaaagt ggcaatggat aaagggttta
    15001 aacattgcca agctggacct ttagtacgta gttcttatca tgcggatgag caagtaaatg
    15061 aagctgctaa agaaaagcaa cgccaaggtg aggcacagtt aaatagttaa tatttaacca
    15121 ttaataaggc ataaaggctt agtttgtaca aaacgaacgt gtcatagaag taatcattat
    15181 ttttatgaaa cactaaaagt ataaactgag cctttcattg tcatagatag gtgaagaatt
    15241 tgataaaagt agatcaacat tactttgaat taatagaaaa ttatcgcgaa tgttttaatg
    15301 aagaacaatt tattgctagg tattcagata ttttagataa atatgattac atagttggtg
    15361 actatggtta cgatcaatta cgattaaaag gtttttacaa agattctaat aaaaaagcag
    15421 agatgagtaa acgtttttca aatattcaag attacatatt tgaatattgt aactttggtt
    15481 gtccttactt tgtattaaga catttgtcta aacaagaggt taaaaagtta atcgaagaag
    15541 ttcatccgtc tgatgtgata gatgacgaca ataaacttca agatgtgaag attaagccaa
    15601 ccattcaaga tactgaacat taataaaacc cttagctaga ttgaaaatgg gaatcatgca
    15661 attcaagcat ggacctgtaa tctagttagg ggtttttatc tttaatgaat gacttcattt
    15721 aaatactcag taatttcatc gccttcttca gcatttacac ctaaaatatg agcgatatag
    15781 ccttcttctt ttaaatcatc agtaccgata ataccgaatt tatttgtttg catattaagt
    15841 acgagtgtct taccataatg tctatttgta tggactaaca tcaaatcata tcgactatgc
    15901 tcgccaacaa aaccaacaaa ctgaacttga ctctcttcgt tgtcatcata taaatacata
    15961 tcaatcattt tgtagcgact ccttttaaaa gtagtaaagt tagtataacg acaaatgaag
    16021 tatactgcaa aattatgata atatataagt gagaggtgac aaggaatgta ttttgtagac
    16081 aaagataaac taactcagaa attagcctat ttacaagcat taactgatga ttatcatgag
    16141 agcaagcaca atcattatgc atttgaacgc attgctcaaa tgttgataga atcatcggta
    16201 gatataggga atatgattat cgatgcattt attttaaggg atcctggtaa ttataaagat
    16261 gtgattgata tattagaact agaaaatgtt attactaaag aaacacagca ggcgattaat
    16321 aaaactgtcg gtattcgtaa acaatttaca tatgattaca cagccttaga tattaagatt
    16381 atcatgccaa tgtttgatga cgcattacct tattacaaac aatttattac agaagtaacg
    16441 acatttttac atcaagaaaa tgtaccagta acagcttttg gtaaaggaga aaatcaataa
    16501 tgaaacagta taaagcgtat ttaatcgatt tagatggcac aatgtatatg ggaacagatg
    16561 agattgatgg agcaaaacaa ttcatcgatt atttaaatgt aaaaggcatt cctcatttat
    16621 acgtaactaa taattcaaca aaaacacctg agcaagtaac tgaaaaatta cgtgaaatgc
    16681 acattgatgc taaaccagaa gaggttgtaa cgtcagcgtt agccactgct gattatattt
    16741 cagaacaatc accaggagca tcagtatata tgttaggtgg gagtggttta aatactgcgt
    16801 taaccgaagc gggacttgtc attaaaaatg acgagcatgt tgattatgta gttattggac
    16861 ttgacgaaca agttacatat gaaaagcttg cgattgcaac gttaggtgta agaaatggtg
    16921 caacatttat ttctacaaat cctgatgtat caattcctaa agagcgtggt ttattacctg
    16981 gtaatggtgc tattacaagt gttgtaagtg tatcgacagg tgtatcgcca caatttattg
    17041 gtaaaccaga accgattatt atggttaaag cattagaaat tttaggatta gataaatccg
    17101 aagttgctat ggtaggcgat ttgtacgata ccgatattat gtctggtatt aacgtaggta
    17161 tggatacgat tcatgtacaa acaggtgtat ctacgttaga agatgtgcaa aataaaaatg
    17221 tgccaccaac gtattctttt aaagatttaa atgaagcaat agctgaatta gaaaaataga
    17281 tatagtcatt ttataaagta ggtgaattga tttggtaaaa atagttgttt cgaggaaaat
    17341 tccagataaa ttttatcaac aattaagtaa acttggtgac gttgttatgt ggcaaaaatc
    17401 attagtgcct atgcctaaag atcaatttgt gacagcactt cgtgacgcag atgcttgttt
    17461 tattacatta agtgaacaga tcgatgcaga aattttagcg caatcaccaa atttaaaagt
    17521 aattgcgaat atggctgtag gatatgacaa catcgatgtt gaaagtgcaa cagcgaataa
    17581 cgtggttgtc acgaatacac caaatgtact tactgaaaca actgcagaat taggatttac
    17641 attaatgctt gctatagcac gccgtattgt agaagctgaa aaatatgtag aagcagatgc
    17701 atggcaaagc tggggtcctt atttattgtc aggtaaagat gtcttcaatt caactattgg
    17761 aatatatggt atgggagata ttggtaaagc ttttgcaaga aggttgcaag ggtttaatac
    17821 taatattctt tatcataatc gatcaagaca taaagatgca gaggcggact ttaatgcaac
    17881 atatgtttct tttgaaacgt tgttagcaga aagtgatttt atcatctgta cagcgccact
    17941 tacaaaagaa acacatcata aatttaatgc tgaagcattt gaacaaatga aaaatgatgc
    18001 aatttttatt aatatcggta gaggacaaat tgtagatgaa acagcattaa tcgatgcact
    18061 agacaataaa gaaattttag catgtggttt agatgtatta gcaaatgaac cgattgatca
    18121 tacacatcca ttaatgggac gtgataatgt tctgattaca ccacacattg gtagcgcatc
    18181 agtaacaaca cgggacaata tgattcaatt atgtattaat aatatagaag cggttatgac
    18241 aaatcaggta ccacatactc cagtaaattg aaaataagta tggtttttaa tcattggtaa
    18301 aacaagcaaa gcaattgtta aatatgttat gcttgacttg cgaatgtata tatttgcgct
    18361 gatgataatt caataacatt ataaactcgt catattaatg tgatgagttt atttgatttt
    18421 gacgtatata acaaaattgt tatatagact gtaattgttt attgtttaaa taacctttaa
    18481 aatgaatgat gttctcaaca aagtgtatca tttacaataa ttgagtgatg acaaccaatt
    18541 actatgttga atctataaaa ttaaaattga atttcattat gaatatcgat ataatgaatt
    18601 gtaaaaaaca aattttaaaa cgtattttat attgaacggt ctttataaag gcgtttttac
    18661 taagaacttt aagaggtgca atatgaaatc taaaagtaaa cagccaccta ataaatatgt
    18721 tgaagcattc aaaccatatt tattaacact attgtatttg gcaatattta ttactttata
    18781 tttaatttat ggcagtggcg acacacacaa taacttcatt tataatgagt tctaatgagg
    18841 gagacttaat atgacagata ttattaacaa gctgcaagcg tttgcggatg caaatccaca
    18901 aagcattgct gttagacaca caactgatga attaacttat caacagttaa tggatgagtc
    18961 tagtaaatta gcacatcgat tacaaggtag taagaaaccg atgattttat tcggtcacat
    19021 gtcaccatat atgattgttg ggatgattgg tgccattaaa gcaggatgtg gatatgtacc
    19081 tgtagacact tcaattcctg aagaccgtat taaaatgatt attaacaagg ttcaaccaga
    19141 gtttgtattt aatacgactg atgaatcatt tgaaagttta gaaggcgaag tatttacaat
    19201 agaagatatt aaaacatctc aagacccagt aatttttgat agtcagatta aagataacga
    19261 cacagtatac acaatcttta catctggttc tactggggaa cctaaaggtg ttcaaattga
    19321 atatgcaagt ttagttcaat ttactgagtg gatgttagaa cttaataaat caggaaatga
    19381 acaacaatgg cttaaccaag cgccattttc atttgattta tctgtaatgg ctatttatcc
    19441 atgtttagca tcaggcggta cattaaatct tgtagataaa aacatgatta ataaacctaa
    19501 attattaaat gaaatgctaa cagcaacacc gattaacatt tgggtatcaa caccatcatt
    19561 tatggaaatg tgtttattat taccaacgct taatgaagaa caatatggta gtcttaacga
    19621 attcttcttc tgtggtgaaa ttctacctca cagagcagca aaagcgttag taaaccgttt
    19681 cccaagtgcg acgatttaca acacatatgg tccaactgaa gctacggtag cagttacaag
    19741 tattcaaatt acacaagaaa tcttagatca atatccgaca ttacctgttg gcgttgaaag
    19801 accaggcgca agattatcta ctacagatga aggtgaactt gttatcgaag gtcaaagtgt
    19861 aagtttagga tacttaaaaa atgaccaaaa aacagctgaa gtatttaatt tcgatgacgg
    19921 tattcgtaca tatcacactg gtgataaagc gaagtttgaa aatggtcaat ggttcattca
    19981 aggtcgtatt gatttccaaa tcaaattgaa tggctacaga atggaattag aagaaattga
    20041 aacacaatta cgccagtctg agttcgtaaa agaagcgatt gttgtacctg tatataaaaa
    20101 tgataaagtt attcatttaa ttggtgcaat tgtgccaacg actgaagtta cggacaatgc
    20161 agaaatgact aaaaatatta aaaatgactt gaaatcacgc ttaccagagt atatgattcc
    20221 tagaaagttt gaatggatgg aacaattgcc attgacttca aatggtaaaa ttgacagaaa
    20281 gaaaattgca gaggtaatta acggatgatt ccatatggtg attttacatt cttcttaatt
    20341 gctttaattg cattattacc agtcattata cttggatttt taggtaagcg aagttacatt
    20401 tataatggcg tagttacagc atttatgatt gtgttaatct tttcttctga taaacataat
    20461 ctgtttgacc aaaagtattt aagtgttcaa ttaattagtt ttattattta cgtcgtatgg
    20521 caagttttat tgataatgtt ttattatcat tcaaaaccaa aaaataattc attttcaaaa
    20581 tttgtaactg taatggtttt atcaatattg ccattagcac ttgtgaaagt gttacaaagt
    20641 acatggttag gtggacatca gattcacttc catgaaagta aattaattga atttgttggt
    20701 ttcttaggaa tttcttatgt tacattcaaa agtgtgcagt taattatgga aattcgtgat
    20761 ggttctatca aagaaattaa agtatggaaa ttaattcaat ttatttcatt cttcccaacg
    20821 atttcatctg gaccaatcga tcgttacaaa cgtttcgtta aagacgataa aaaagtacca
    20881 acaggcaatg aatatcgtga attagtatta aaagcaattc acatgattat gcttggtttc
    20941 ttgtataaat atattgttgc ttactttatt aacacatatg caatcatgcc gttacaatta
    21001 gacttacatg gctttgtcaa tttgtggtta tatatgtacg catacagctt atatttattc
    21061 tttgactttg caggttatag tttatttgcg atagcattta gttatttatt cggtattaaa
    21121 acaccaccaa acttcgataa acctttcaaa gcgaaaaata ttaaagattt ctggaataga
    21181 tggcatatga cattatcatt ctggttcaga gattgtattt acatgagatc tttattctac
    21241 atgtctcgta aaaaattatt gaagagtcaa tttgcaatgt ctaacgtggc attcttaatc
    21301 aacttcttca taatgggaat ttggcatggt atcgaagtgt attacattgt ttatggttta
    21361 taccatgcag cattgtttat aggttatggc tattatgaac gttggcgtaa gaaacatccg
    21421 ccacgttggc aaaatggttt cacaacagca cttagcattg tgattacatt ccactttgta
    21481 acatttggct ttttaatctt ctcaggtaaa cttatataat aaaggagaat ttaattatgg
    21541 aatttagaga acaagtatta aatttattag cagaagtagc agaaaatgat attgtaaaag
    21601 aaaatccaga cgtagaaatt tttgaagaag gtattattga ttctttccaa acagttggat
    21661 tattattaga gattcaaaat aaacttgata tcgaagtatc tattatggac tttgatagag
    21721 atgagtgggc aacaccaaat aaaatcgttg aagcattaga agagttacga tgaaattaaa
    21781 acctttttta cccattttaa ttagtggagc ggtattcatt gtctttctat tattacctgc
    21841 tagttggttt acaggattag taaatgaaaa gactgtagaa gataatagaa cttcattgac
    21901 agatcaagta ctaaaaggca cactcattca agataagtta tacgaatcaa acaagtatta
    21961 tcctatatac ggctctagtg aattaggtaa agatgaccca tttaatcctg caattgcatt
    22021 aaataagcat aacgccaaca aaaaagcatt cttattaggt gctggtggtt ctacagactt
    22081 aattaacgca gttgaacttg catcacagta tgataaatta aaaggtaaga aattaacatt
    22141 tattatttca ccacaatggt ttacaaacca tggtttaacg aatcaaaact ttgatgctcg
    22201 tatgtctcaa actcaaatta atcaaatgtt ccagcagaaa aacatgtcta ctgaattaaa
    22261 acgtcgttat gcacaacgtt tattacagtt tccacatgta cacaataaag aatacttgaa
    22321 atcttatgct aaaaacccta aagaaactaa agatagttat atttctggtt ttaaagagaa
    22381 tcaattgatt aaaatagaag cgattaaatc attgtttgca atggataaat ctccattaga
    22441 acatgttaaa cctgctacaa aaccagacgc ttcttgggat gagatgaaac aaaaagcagt
    22501 tgaaattggt aaagctgata ctacatcgaa taaatttggt attagagatc aatactggaa
    22561 attaattcaa gaaagtaagc gtaaagttag acgtgactac gaattcaatg ttaattctcc
    22621 agaattccaa gatttagaat tacttgtaaa aacaatgcgt gctgctggtg cagatgttca
    22681 atatgtaagt attccatcaa acggtgtatg gtatgaccac attggtatcg ataaagaacg
    22741 tcgtcaagca gtttataaaa aaatccattc tactgttgta gataatggtg gtaaaattta
    22801 cgatatgact gataaagaat atgaaaaata tgttatcagt gatgccgtac acatcggttg
    22861 gaaaggttgg gtttatatgg atgagcaaat tgcgaaacat atgaaaggtg aaccacaacc
    22921 tgaagtagat aaacctaaaa attaaaatac aaatagcaca taactcaacg attttgattg
    22981 agcgtatgtg ctatttttat attttaaatt tcatagaata gaatagtaat atgtgcttgg
    23041 atatgtggca ataataaaat aattaatcag ataaatagta taaaataact ttcccatcag
    23101 tccaatttga cagcgaaaaa agacaggtaa taactgatta taaataattc agtattcctg
    23161 tctttgttgt tattcataat atgttctgtt aacttaatat ctttatatta gaatacttgt
    23221 tctacttcta ttacaccagg cacttcttcg tgtaatgcac gctcaatacc agctttaaga
    23281 gtgattgtag aacttgggca tgtaccacat gcaccatgta attgtaattt aacaataccg
    23341 tcttccacgt caatcaatga gcagtcgcca ccatcacgta ataaaaatgg acgaagacgt
    23401 tcaataactt ctgctacttg atcaaacatc gttgtatctt cagtaggcat caggcaagtc
    23461 tcctttcgag agatgaattt gataatcttt attagtatat tataatagat agtgtaataa
    23521 aaatctatca aaccaatggg ggataaatat atggagcacg tgagtgtggt agtttatggg
    23581 gcagatgtta tatgtgcaag ttgcgttaat gcgccaacat cgaaagatat ttatgactgg
    23641 ctacagccgc tattaaaaag aaaataccca aatatatcat ttaaatatac gtatatagat
    23701 attacaaaag ataatgacaa cttaacagat catgatttac aatttattga aagaatagaa
    23761 caagatgaac tattttatcc attaattaca atgaacgatg aatatgtagc agatggttat
    23821 atacaaacaa agcaaatcac tcgatttata gaccaaaagc ttgtgaatga ataatataca
    23881 cataactaaa agcaatgtca gtaatagtgt gaactgacat tgctttttaa ttttaaccat
    23941 tgtgatattt atataaccaa agtacgccgg attttaaaat agaagcgagt cgtcctgtta
    24001 cagtgcggtc catgatatat gcaaaacctt gcttatctcc taatgaccca acgatacctt
    24061 gtacctttag ttccggcatt ttgtcaggta atggttcatt tagccattgc tttttaagca
    24121 catcggcaat ttgatcacct tgaacttcgg ctaactgagc acttggcgca tgtggtaaat
    24181 cagcacaatc accaactaca tagacgttac gatatgttgg tacttgatga tactggttaa
    24241 ctatcacgcg tccattacta tttatatcaa tcggcaagtt acgaacaact tcaacaggtt
    24301 gaattcctgc tgtccataca actaaatcaa tatctttagg ttcatcacag ttatatattt
    24361 taccaggttc aactttatta atatttgaat ttggaacaac ggtaacatta tttttggcga
    24421 accatttcgc aacatactta cttaattttt ctggaaaatt tcttaaaatt cgcggcccac
    24481 ggtcataaag atatatttcc aagtctgatc tactttctct taattcgctg gcaagttcta
    24541 tgccgcttaa tccagcacca acgataccga ctttagcacc ttctggtagt tcactaatac
    24601 tatggaaagt atcccgagcc tttgagagtg tttgaatact atgtgtatat tcttcggctc
    24661 ctggaacgtt atgatattta tcttcacatc ctaaaccaat gattagctca tcataatcaa
    24721 ttttagaatt accgactgag acaatttgag catctaaatc tatgtcgtta atttcaccat
    24781 aaactgtatt cacttgtgga tgattaggga atttcatacg aacatcttta tctgatttcg
    24841 tgcccgcagc taaagcataa aattctggtt tcaatccatg aaatggcata cgatcaacta
    24901 atgtgactgt ataatcttgt ggtaaagaag tagttaaaat gcgtgacatg atacgcatat
    24961 taccatatcc gccgcctaac aaaactaagt ttttcataat gaagtacccc ttttatatgt
    25021 taatatttac tttaattttg aaaagatcaa agtgcataga tatatttgtt aatgaacagt
    25081 tataaaatgc aagtgtcttt gattcatcaa taacccctaa aaaagtaaat atgtatttat
    25141 atgcttatta tatatttttt tgaaaatgac tacaaataaa tatttgtact aactaaggca
    25201 ctattacata actgggataa gaaacatcag ttgttgctaa ttgagcacat catgttaaaa
    25261 taccagtttt aaaatgctat aatattgtaa caaatgaaat aagaaggtga agtatgaatc
    25321 cgatcgtaga attttgtctc tctaacatgg caaaaggtgg agattatgtt tttaatcaac
    25381 tggaaaatga cccagatgtc gatgtgttag agtatggttg cctgacacat tgtggtatat
    25441 gttcagccgg gttgtatgct ttagtaaatg gtgatattgt tgaaggtgat tcgccggaag
    25501 aattattaca aaatatatat gcacatataa aagaaacttg gattttttaa ggaggagtta
    25561 tcatgccaac agttatatta acagaagcag ctgcttacga agtaaaagat atgcttaaag
    25621 caaatgaaat gccagatggc tatttaaaaa ttaaagtgaa tggtggcggg tgcactggtt
    25681 taacatacgg tatgggtgca gaagaagcgc ctggtgaaaa tgatgaagtc ttagaatact
    25741 ttggattaaa agtattagta gacaaaaaag atgcacccgt attaaatggt acgactattg
    25801 attttaagca atcattaatg ggtggcggtt tccaaatcga caatcctaat gcaattgctt
    25861 catgtggctg tggtagttca tttagaactg caaaagttgc aggtaatcct gaaaattgct
    25921 aataaaataa ctttttaatg gttaacccaa tttttgggat acaaattgat atcaataacg
    25981 attaaggaca tatttgatta tgtcctattt ttgcgtaaag atattttgtt ttattaaaaa
    26041 gcttattgaa aattaagaat ctgactataa atagtatttt aaatgatcgt gatagcggat
    26101 acatttttaa tcgaaatcag gctacgaaaa aattaattaa ttttttcaca atctgactga
    26161 atcttgcttg aaaatataac ataaaagcta tagaattagt attggattaa aaatgtcttt
    26221 tttgtgaaca tcacaggttt ttacataatt taaaagactt aaataacaaa atgatgataa
    26281 actagtatac attgtggtaa aagtagtaat gaccagttgt tactagtaat tatcagcatt
    26341 tggttaaaaa aatatgaaag cttaggtgaa attaaatggc tcaagatcgt aaaaaagtac
    26401 ttgtacttgg tgctggttat gcaggtttac aaactgtaac taaattgcaa aaagcgatat
    26461 caacagaaga agcagaaatt acgcttatta ataaaaatga atatcactat gaagcaacat
    26521 ggttacatga agcatcagca ggtacactaa actatgaaga tgtattatat cctgtggaaa
    26581 gtgtcttgaa gaaagacaaa gtgaactttg ttcaagcaga agtaacaaaa attgaccgtg
    26641 atgctaaaaa ggtagaaaca aatcaaggta tttatgactt tgatatttta gtagtagcat
    26701 taggtttcgt tagtgaaaca ttcggcatcg aaggtatgaa agatcatgct ttccaaattg
    26761 aaaatgttat cacagcacgt gaattatcac gtcatatcga agacaaattt gctaactatg
    26821 cagcatcaaa agaaaaagat gataacgatt tatctatctt agttggtggt gctggattca
    26881 ctggtgttga attcttaggt gaattaacag acagaattcc tgaattatgt agcaaatatg
    26941 gtgtggatca aaataaagtt aaaatcactt gtgttgaagc agcacctaaa atgttaccaa
    27001 tgttctcaga agaattagtt aaccacgcag ttagctactt agaagaccgc ggtgttgaat
    27061 ttaaaattgc tacaccaatc gttgcttgta acgaaaaagg ttttgtagtt gaagtagatg
    27121 gtgaaaaaca acaattaaat gcaggtactt cagtatgggc agctggtgta cgtggtagta
    27181 aattaatgga agaatcattt gaaggcgtta aacgtggacg tatcgttaca aagcaagatt
    27241 taacaatcaa tggttacgac aacatttttg ttattggtga ctgttcagcg tttatcccag
    27301 ctggagaaga acgtccatta ccaactacag cacaaattgc aatgcaacaa ggtgaaagtg
    27361 ttgctaaaaa cattaaacgc atcttaaacg gtgaatcaac tgaagaattc gaatacgttg
    27421 atcgtggaac tgtttgttct ttaggttcac atgacggtgt aggtatggta tttggtaaac
    27481 ctatcgctgg taaaaaagca gcattcatga aaaaagtgat tgatacacgt gcggtattca
    27541 aaatcggtgg tatcggttta gcattcaaaa aaggtaaatt ctaggcttta aataaagttt
    27601 cagctaaaca cataaaatta aattattcat ttataaagtc cttcagtgtc actgaagggc
    27661 tttattgttg tacaatttga agtgagtaac atatcaaatc taaggaggta cattatgaat
    27721 tttaaattaa ataacacact aagcaacgaa ataaatacat tgattattgg tataccagaa
    27781 catttaaatc agttagagcg cattagtttt aatcatatcg atattacaga atcacttgaa
    27841 agactaaaac atcaacatat tattggtagt aaagttggga agatttatac aactgcattt
    27901 gatgtacaag atcaaacata tcgtttaatt acagttggtt taggaaactt aaagacacgt
    27961 agttatcaag atatgttgaa aatatgggga catcttttcc aatacataaa gtcagaacac
    28021 attgaagata cgtatttact tatggattca tttatttcaa aatatgatca gttatcagat
    28081 gtattaatgg catgcggtat tcaaagtgag cgtgcaacat atgaattcga tcattataaa
    28141 tcaagtaaga aggcaccgtt taagacgaat ttaaacctta ttagtgaatc attaattgaa
    28201 ttagatttta ttcatgaggg tatcagtatt ggccaatcca ttaatttggc aagagacttt
    28261 agtaatatgc caccgaatgt attaacacca caaacatttg cagaagatat tgttaatcat
    28321 tttaaaaata caaaggtcaa agtagatgtt aaagattatg acactttagt ttctgaagga
    28381 ttcggacttt tacaagcagt aggtaaaggt agtaagcata aaccgagatt agtaaccatc
    28441 acatataatg gcaaagacaa agatgaagca ccaattgcct tagttggtaa aggtataacg
    28501 tatgattctg gtggttatag tattaaaacg aagaatggca tggctacaat gaagtttgac
    28561 atgtgtggcg ctgcgaatgt cgttggtatc attgaagcgg ctagtcgttt acaactgcct
    28621 gtaaatattg tcggagtgct tgcgtgtgct gaaaatatga taaatgaagc atcaatgaag
    28681 ccagatgatg tatttacagc attaagtggt gaaactgtag aagtaatgaa tacagacgct
    28741 gaaggtagat tagtccttgc agatgctgtg ttttatgcaa atcaatatca gcctagtgtg
    28801 attatggact ttgctacatt aacgggtgca gcaattgttg cactaggcga tgataaagct
    28861 gctgcatttg aatcgaatag taaagtgata ttaaacgata tattacaaat aagttctgaa
    28921 gtcgatgaaa tggtatttga attaccgatt actgcaaccg aacgtgcaag tattaaacac
    28981 agtgatatcg ctgatttagt taaccatacg aatggacaag gtaaagcgct atttgcggca
    29041 agttttgtaa cacattttag tggtcaaaca cctcacattc atttcgatat tgcaggtcca
    29101 gcaacgacta ataaagcttc atataatggt ccaaaagggc caacaggatt tatgattccg
    29161 acgatagtac aatggttaaa acaacaataa tttaattatt tgcctgatac aacttgtgtc
    29221 ttgatataca tttctaattt aactcttgaa agtataaagg atcgcattgt atcaggcatt
    29281 ttcattgttg taagaaaata taaatcgcat atccttgtga gcataaatca acttgactag
    29341 cattgcaaaa aaatattata agcatatgtt cattcagtca acgactacta atattgcatt
    29401 ttagaactta ggattttgat tggtgttcca agcttgtaaa attgtttaat ttaatgaaag
    29461 ctaaaattat tttaagaaag acaattaact aaaaaataaa gattattggt atagtttgct
    29521 attgacaggt gttaaagcta ctgatactat gtagtctata aacgctttag ttcgatagag
    29581 tgataaagga tggttgtcat atgataaatg cagtagtaat agcagtaatt ttaatgattg
    29641 tgctatgttt atgtcgatta aacgtagtta taagcttatt tatcagtgcg ctagttggtg
    29701 gcttaatttc aggcatgagc attgaaaaag ttataaatgt atttgggaaa aatatagtcg
    29761 atggtgctga ggtagcatta agctatgctt tattaggtgg atttgcagca ttaatttcat
    29821 acagtggtat cacagactat ttagtaggaa aaattataaa tgcaattcac gctgaaaata
    29881 gtcgatggtc aagagttaaa gtcaaagtga caataatcat tgcattatta gctatgagta
    29941 tcatgagtca aaacttaatt cctgtacata ttgcattcat tccaattgtc atcccaccat
    30001 tgttaagtct gtttaatgac ttaaaaatag atagacgttt aatcggtttg attatcggtt
    30061 ttggtttatg tttcccgtat gtgttattac catatggatt cggtcaaatt ttccagcaaa
    30121 ttattcaaag tggctttgca aaggcaaatc acccaattga gtttaatatg atttggaaag
    30181 caatgcttat tccttcaatg gggtatattg ttggcttact tatcggttta tatgtatatc
    30241 gtaaaccacg tgaatatgaa acacgtaaaa tttcagatag tgacaatgtt acagagttaa
    30301 aaccatatat cttaatagta acaattgtag caatactagc tacattttta gtacaaacat
    30361 ttacagattc aatgattttt ggtgcactgg caggggtact cgtattcttt atttcacgtg
    30421 catataattg gtatgaatta gatgctaagt ttgttgaagg tattaaaatt atggcttata
    30481 ttggtgtagt tattttaaca gcaaatggat ttgctggtgt aatgaatgct actggtgata
    30541 tagatgaatt agttaaaact ttaacaagta ttactggtga taataaatta tttagcatta
    30601 tcatgatgta tgtgataggt ttaattgtca ctttaggtat tggatcatca tttgcaacaa
    30661 ttcctattat cgcatcatta ttcattcctt ttggagcgtc aattggacta gatacaatgg
    30721 cattaatcgc attgattgga acagcgagtg cattaggtga ctcaggttcg cctgcaagtg
    30781 attcaacatt aggaccaact gcgggattaa atgttgatgg ccaacatgat catatacgtg
    30841 atacatgtgt accaaacttc ttgttttata atattccttt aatgattttc ggtactattg
    30901 ctgctatggt actataagca taaagaggtg aaaatatgac tcatttatta gagacatttg
    30961 agatgtcaat agatcaccag gaagatggtt tagttgttat ttctatgcct gttactgata
    31021 aagtaaaaca accatttgga tatttacatg gtggggcttc gattgcttta ggtgaaacag
    31081 catgttcatt aggatctgct aatttaattg atacaaccaa atttattcca ttaggtttag
    31141 agatgaatgc taaccatatt cattctgcta aagatggtcg tgttactgcg acagctgaaa
    31201 ttattcatcg aggtaagtcg acacatgtat gggatataaa aattaagaat gacaaagaac
    31261 aattaattac agttatgcgt ggtacagttg ctattaaacc tttaaaataa aagaactgct
    31321 agctgaaatg ttatgagata ttcataacta cggctagcag tttttttatg cgctatattg
    31381 ttgtagtttt agaaatgctt gttcaatgcg ttcggcagct ttacggccac ccataacatt
    31441 tctaccaaat ggtcctaatt ctaagtctgc aaagcatcct gcgacaaata gatttggtat
    31501 ccattctaat ttttcggaaa taacagggta attacattcg ttgataggtg catcataatt
    31561 ttgtattaat tgcttaataa gtggttgtga cataaaatct tgttcaaaac cagttgcaac
    31621 cataatctgt tgatatggaa cagaatcatt ttcagtgtta attacaccac cactaatttg
    31681 agtgataggt gttttatgca catttatacg accattttta atatgttttt taaggcgtaa
    31741 gtacagttcg tgaggcattg atcctttatg acgttcgcgt tgtacaatgg catttctttc
    31801 aggcatgctt ttagtactta aaaatgaaga catatttttc ggacctaacc aaccaggatc
    31861 agcatcaaag tcatgtattt caatatcttt atttagccat aaatgaatct ttttatcgtt
    31921 atcatgattt aacaatttaa gtgcaagatg tgcagcagta atgccgctac caacgatatg
    31981 atcggtctta tcatatacta cttgatcaag ttctttctcg aagatatgat ttacattctg
    32041 tttgtctttt aaaatgtcag gcataaacgg aatatttgta ctgcctattg caataacgac
    32101 gcaatctgta gtgataattt gtccatcttc taacttgata tgccatttgt cttcttgttt
    32161 atctaaagtt tgaactaaac cttgaaccaa gcaatcctct aattgatatt gtttagaagc
    32221 atgtgcaata tgatccataa acattgtcaa ttcaggtcgt tgataaggac cataaaaagc
    32281 atttgtatat tggtgctgtt tagcgaattg ttttagatgg aacggttgtg gatgtacgtg
    32341 atgtacaatc ggtgatctta aataaggcat ttctattcga tttgtatatg agttaaacct
    32401 ttggcaaaaa gtttcgtgtg ggtcaatgat tgttaatcgg tctgttgtta atccgcttga
    32461 taatagtttt tgtgcgattg cagttccctg tatgccaccg ccgataattg tccaatgcat
    32521 aataaaacct ctctcttttt aaaacgtaat cgttacgatt tataattatt attatcataa
    32581 tacataacga catgaaaggc aattaaatta aagagatata tgtagatagg gcgaatctgt
    32641 agtcaaagaa aaaatcattg aaaaagaggt aacaatgtca aaagaaaaca gcagtaaaat
    32701 cattcctaat ttggaatcat cttactgctg tttgttgttg atttatattc atgattttgt
    32761 tatataatct acaattttgt gtcttttaag tcttccgaaa tttcatcgac tttagtcttt
    32821 ttagtataag gcgttttaat attatatgct gctttcataa tcatatgact tgaaagagga
    32881 cctgtaatta atacaaagat aatcgcaacg attaattgca tatttacaaa accttgagta
    32941 gcaataaaat ataaaaacgt accaaatagt aatgacattg cacctaatgt tgatgctttt
    33001 ccggcagcat gtgcacgtga atatacatct tcaagtctca ataatcctat agctgctagg
    33061 gcgctaatta aagcaccgat gataacaaag ataagtgcaa gactaatcag tatgattttg
    33121 atcatgttca atcaccttac ctttgtccat aaatttagag aatactgcag tacctaaaaa
    33181 agctaatata ccaatcatca taataacgac aatcatgtat ttaatattta ataaaatact
    33241 gaataatgct ataactgcca ttaattgaag accaatcgca tctaatgcga caacacgatc
    33301 ggcaagtgat gggcctagca caacgcgaat gagcatagct aacatagaaa tgacaactat
    33361 gattaatgca ataacgataa taacattatg attcattata tttcgcccac ctctcttaca
    33421 attttctcta atgatgtttt aatactttct acttcttgct ctttagttga aaaatctatg
    33481 gcatgaatat aaatttttgt acgatcgtca cttacaccaa gcactacagt accaggtgtt
    33541 aatgtaatta aattagacag caagacaatt tgccaatctt tttttaaatc tgtgtgataa
    33601 acaaagaatc ctggttcatt tttaatcgaa ggtttaataa taattttcaa aacatcaaaa
    33661 ttagctttaa tcagttcgat taagaaaata ataactaatt taataatacg atatagcgtg
    33721 atgacataaa atctacctgg taacactctg tgtaagaggt aaacaagaac taggccaaag
    33781 atgaaaccta acacaaagtt atttgttgtg taactatttg tcacaaacaa ccaaaacact
    33841 gcgataataa agtttaatac taattgtaca gccatgttat ttacctccta atacagcttt
    33901 aacgtaggtt gatggattgt agaatgtttc tgcaccagct tttaccattg gatataagta
    33961 atctgctgac aatccatata aaacagttat cacaactgca acgattgcaa tcgtagttaa
    34021 atatttgacg tcgactttgt tattaagatc atatcctttt ggttgaccga aaaagccttg
    34081 taggaatatg cgaatgacag aatataatac gactaaactt gataataaga cgatgacacc
    34141 acttaaataa aatcctcttt caaatgttga ttggacaata aaaaattttc cataaaagcc
    34201 actgagtggg ggaatgccag ctaaacttaa tgctgcgata aagaatgacc aaccaagtac
    34261 aggatatcgt ttaattaagc caccaaattg tcttaaatca gcagtgcctg taattttaat
    34321 cataattccg ataagcaaga ataatgcaag ttttactaac atgtcgtgca atgtatagta
    34381 aatagcccca atcatacctg actctgtcat cattgcaacg ccgactaaga tcacacctac
    34441 agcaatcatg acattgtata ggatgatttt tttaatgttg gcatatgcaa cagcaccgac
    34501 acaaccaaag atgatcgtta atagtgctaa gaataaaatg acataatgtg aaaagcttac
    34561 attatcacta aagaataggc tcaatgttct agcgattgca taaacaccaa cttttgttaa
    34621 caaagcacca aagaatgcaa tgattggaat tggtggtgca tagtatgcac taggtaacca
    34681 aacaaacatt gggaatacgc cagcttttgt agcaaaaaca aagataaata gtatgaaaac
    34741 gatattgact aagccactgt catgcgctga aaggttagct aatttattgc ttatatctgc
    34801 tagattcaat gttcctacta ctgaatataa aatcgctaca cccattacga agaaggatga
    34861 cgatacaacg ttaacaagaa catattttat tgtttcttgt agttgaattt ttgtagaacc
    34921 aattactaat aagaaataag atgacattaa aaatacttcg aaatatacga ataggttgaa
    34981 aatgtcacca gttgtgaatg caccaatgat acctattaac ataaatagta ctgaaaaata
    35041 ataataatat ctttcacgtt caataccaat tgtttggtat gaatataaaa tcacaatagc
    35101 tgtaataata atactagtaa ttattagtag ggcactgaat atgtctaata caaagacaat
    35161 actgtatggt gctttccatg aacctagctc tacgcgtatt ggtccatgtt taacaacatt
    35221 tgctaaattg ataattgccg cgaccaaggt taataatgta ccgcctagtg cgacataacg
    35281 ctttataata ggacgctttc caataaagac aagtaatatg gctgtaatta ctggaataac
    35341 tagcgttaac acaagcatat tactttcaat catcttctgg aactcctttc atactctcaa
    35401 cgttatctgt gcctaattct ttatatgttc taaatgctaa tactaagaaa aaggctgttg
    35461 tcgcaaaggc gataacgatt gctgttaaaa taagtgcttg cgggatagga tcaacatagc
    35521 tttttacgtt cgcttcataa attggaacag taccatgttt aagtccgccc atagttatta
    35581 aaaataaatt tgctgcatgt gttaatagtg tagttcccat aacaattcgt atcagacttt
    35641 tagacaaaac gagatagaca ctaattgctg tgagaatacc actaacaaaa atcataataa
    35701 tttccactat tcgttctctc caatcgaaat aataattgtc atgacagtac caactactgc
    35761 acataaaaca ccgaaatcaa agaatactgc tgttgtcata tgaacaggtt ctaatataaa
    35821 taacggtata tcaaatgtga catgcgtaaa gaaatttttg cctaaaaacc aacttgcgat
    35881 aggcgtcgca atacaaaaaa ctaatccgat acctatcaag attttaaaat ctaatgggaa
    35941 aattttacgc attgtttcta tatcaaatgc aatcgtaatg ataacaagtg aacttgcgaa
    36001 taataatccg ccgacgaaac cgccaccagg tgtataatgt cctgctaaga aaagtgaaaa
    36061 accaaagacc attaccatga aaaagataat aactgcagca aattgcaaaa ttagatcatt
    36121 ttgttgtcta ttcatgattt ttcacctcgt taccttgcgt ttgacgcttt ttacgtaatt
    36181 taatcattgt atatacagct aatcctgcga taccaagcac agatgactcg aataaagtat
    36241 ccataccacg gaaatcaaca agtatgacgt ttaccatgtt tttaccgtga gctaaatcat
    36301 aaacgtgctc ttgataaaac ttagatatcg attcaaaatg tctatttccg tatgcaatta
    36361 aaccgataat aatgacggac aaaccaacac caccagcaat taaagcatta gtaagctgga
    36421 atgagcgctt ttcattataa cgatttaaat ttggtaagtg gtagaagcat aataagaaca
    36481 atgctgttga aatagattca acgacaaact gtgtcaatgc taagtcgggt gctttaaaga
    36541 atataaacaa tacagacaca gcatatccaa ctgcacttaa cataatgatg ctaaataatc
    36601 ttgatttagc gaaaagaatt aaaaaggcag cacttaataa taaaattacg atacaaactt
    36661 cgaaaattct aatcggacta acgtctttaa aattaatgtt gaaaggtact gagaatatag
    36721 tgacaaatgt taataaaatt aatgcaccaa aaatgataac taaattatta cgtgaataat
    36781 cggtaacata gctattcgtc atcttttcag agtagtttgg aataacattt gcacttctgt
    36841 tgtaccaata attgaatgtt agtttaccag gttgtcgttg caacaatttc acccaataac
    36901 taaatgtcac aattagtaag atacctaaaa tataaatcac taatgttgat aaaaaggcag
    36961 gcgttaatcc atggaacata tggaattcaa catcatcaat taccgtatga ttaatcgaag
    37021 atgtagctgg ttcaataatc gaattagtta aaatgccagg gaataaacca aatacaatta
    37081 ctaatgtagc taaaatagct ggtgataaaa gcattaatat tgatacttcg tgtgcttttt
    37141 taggtaattg ttcaggttta tattgtccga aaaatatatg cattataaat ttaattgaat
    37201 atacaaatgt gaagacactg cccactatac caatgattgg gaataggtag cctaatgtat
    37261 caacactgaa taaatttgct tggcttgctg taaatgttgt ttctaaaaat gattcttttg
    37321 ataagaaacc attgaacggt ggtacaccag ccatacttaa tgctgtaata acagtgattg
    37381 taaatgaaat aggcataatt gttagtaagc cacctaattt cttaacatca cgtgtaccag
    37441 tagaatgatc cactgcacct gtaatcataa atagggcacc tttaaatgtt gcatggttga
    37501 ttaaatggaa tattgcagcc gtaaatgcag cagcatatat tttgctatca tcgccttgat
    37561 agtgataact aatggcaccg attccaagca tcgccataat catacctaat tgggatactg
    37621 ttgaaaatgc cagtatacct ttcaagtctt gttgttttgt tgcgtttagc gaagcccaga
    37681 ataatgtaat taaaccaacg agtgtgacag tccataccca accttgcgat gctgcgaaga
    37741 ttggtgtcat tcgagcgatt aaatataacc ctgctttaac cattgttgct gaatgaagat
    37801 aagcactgac tggtgtaggt gcttccattg catctggtag ccaaatataa aatggaaact
    37861 gagcagattt tgtaaaagca ccaatcatga ttaaaatcat cgcaaaaatg aagaatgggc
    37921 tattttgaat ttcagaagca tgttgaatca tgtactgaat gctaaatgat tgtgttggta
    37981 tagcgagtaa gatgatacca cctaataatg atagaccacc aaatactgtg attatgagcg
    38041 atttttgagc accatatata gatgcttgtc gttcgcgcca gaatgaaata agtaaaaaac
    38101 tagaaaatga cgttagctcc cagaataaat atagaataat aacattatct gaaa
//
LOCUS       DANRDI010000010        34341 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.010, whole genome shotgun sequence.
ACCESSION   DANRDI010000010 DANRDI010000000
VERSION     DANRDI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.010"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(140..553)
                     /locus_tag="PZ377_000335"
     CDS             complement(140..553)
                     /locus_tag="PZ377_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357227.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(582..692)
                     /locus_tag="PZ377_000336"
     CDS             complement(582..692)
                     /locus_tag="PZ377_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357228.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            complement(778..1509)
                     /locus_tag="PZ377_000337"
     CDS             complement(778..1509)
                     /locus_tag="PZ377_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDM6357229.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(1511..2824)
                     /gene="murT"
                     /locus_tag="PZ377_000338"
     CDS             complement(1511..2824)
                     /gene="murT"
                     /locus_tag="PZ377_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDM6357230.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            3116..3616
                     /gene="ftnA"
                     /locus_tag="PZ377_000339"
     CDS             3116..3616
                     /gene="ftnA"
                     /locus_tag="PZ377_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDM6357231.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3698..3790)
                     /locus_tag="PZ377_000340"
     CDS             complement(3698..3790)
                     /locus_tag="PZ377_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357232.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            4005..4559
                     /locus_tag="PZ377_000341"
     CDS             4005..4559
                     /locus_tag="PZ377_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDM6357233.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(4627..5697)
                     /gene="dinB"
                     /locus_tag="PZ377_000342"
     CDS             complement(4627..5697)
                     /gene="dinB"
                     /locus_tag="PZ377_000342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDM6357234.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(5944..6474)
                     /locus_tag="PZ377_000343"
     CDS             complement(5944..6474)
                     /locus_tag="PZ377_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDM6357235.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(6644..8005)
                     /gene="rlmD"
                     /locus_tag="PZ377_000344"
     CDS             complement(6644..8005)
                     /gene="rlmD"
                     /locus_tag="PZ377_000344"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDM6357236.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(8086..9033)
                     /locus_tag="PZ377_000345"
     CDS             complement(8086..9033)
                     /locus_tag="PZ377_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDM6357237.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            complement(9616..9762)
                     /locus_tag="PZ377_000346"
     CDS             complement(9616..9762)
                     /locus_tag="PZ377_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357238.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            complement(9802..11229)
                     /gene="gatB"
                     /locus_tag="PZ377_000347"
     CDS             complement(9802..11229)
                     /gene="gatB"
                     /locus_tag="PZ377_000347"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDM6357239.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            complement(11242..12699)
                     /gene="gatA"
                     /locus_tag="PZ377_000348"
     CDS             complement(11242..12699)
                     /gene="gatA"
                     /locus_tag="PZ377_000348"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDM6357240.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            complement(12701..13003)
                     /gene="gatC"
                     /locus_tag="PZ377_000349"
     CDS             complement(12701..13003)
                     /gene="gatC"
                     /locus_tag="PZ377_000349"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDM6357241.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            13370..14908
                     /gene="putP"
                     /locus_tag="PZ377_000350"
     CDS             13370..14908
                     /gene="putP"
                     /locus_tag="PZ377_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDM6357242.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            complement(14997..16196)
                     /locus_tag="PZ377_000351"
     CDS             complement(14997..16196)
                     /locus_tag="PZ377_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDM6357243.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(16209..18212)
                     /gene="ligA"
                     /locus_tag="PZ377_000352"
     CDS             complement(16209..18212)
                     /gene="ligA"
                     /locus_tag="PZ377_000352"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDM6357244.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            complement(18216..20408)
                     /gene="pcrA"
                     /locus_tag="PZ377_000353"
     CDS             complement(18216..20408)
                     /gene="pcrA"
                     /locus_tag="PZ377_000353"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDM6357245.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            complement(20405..21097)
                     /locus_tag="PZ377_000354"
     CDS             complement(20405..21097)
                     /locus_tag="PZ377_000354"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDM6357246.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            complement(21269..21571)
                     /locus_tag="PZ377_000355"
     CDS             complement(21269..21571)
                     /locus_tag="PZ377_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357247.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            complement(21680..22975)
                     /gene="purB"
                     /locus_tag="PZ377_000356"
     CDS             complement(21680..22975)
                     /gene="purB"
                     /locus_tag="PZ377_000356"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDM6357248.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            23836..25002
                     /gene="scpA"
                     /locus_tag="PZ377_000357"
     CDS             23836..25002
                     /gene="scpA"
                     /locus_tag="PZ377_000357"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDM6357249.1"
                     /translation="MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            25033..25359
                     /locus_tag="PZ377_000358"
     CDS             25033..25359
                     /locus_tag="PZ377_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDM6357250.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(25651..25824)
                     /locus_tag="PZ377_000359"
     CDS             complement(25651..25824)
                     /locus_tag="PZ377_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDM6357251.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(25805..26407)
                     /locus_tag="PZ377_000360"
     CDS             complement(25805..26407)
                     /locus_tag="PZ377_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDM6357252.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(26678..27499)
                     /gene="nadE"
                     /locus_tag="PZ377_000361"
     CDS             complement(26678..27499)
                     /gene="nadE"
                     /locus_tag="PZ377_000361"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDM6357253.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(27492..28961)
                     /locus_tag="PZ377_000362"
     CDS             complement(27492..28961)
                     /locus_tag="PZ377_000362"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDM6357254.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            29145..30221
                     /locus_tag="PZ377_000363"
     CDS             29145..30221
                     /locus_tag="PZ377_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDM6357255.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            30241..31035
                     /locus_tag="PZ377_000364"
     CDS             30241..31035
                     /locus_tag="PZ377_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDM6357256.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            31107..31214
                     /locus_tag="PZ377_000365"
     CDS             31107..31214
                     /locus_tag="PZ377_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357257.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(31225..32787)
                     /locus_tag="PZ377_000366"
     CDS             complement(31225..32787)
                     /locus_tag="PZ377_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDM6357258.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(32992..34089)
                     /locus_tag="PZ377_000367"
     CDS             complement(32992..34089)
                     /locus_tag="PZ377_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDM6357259.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
BASE COUNT        10820 a         6248 c         4863 g        12410 t
ORIGIN      
        1 gcctcttttt atcgtatata gcatgtccct ttttaaaatt acaagcataa atattcagac
       61 tcaaatatag ccacacgcct aaactacgga gggatgtggc tgtctttttt gattgtaatt
      121 acatatcaga tttaaatcat taatgtaaag aggctaattc attaataaat ttagtaaaat
      181 ctgatttagc aacatttatc cttaatgaaa taccagattc tgttgccatg tttgaatgcc
      241 ttaaaccaga atcaaaatca atataaatac aaagcagttc ttgattaaaa tcaataattg
      301 taaaatgtaa atcaggttct ataaaatata tttcagtaac aggattgtcg caatccatat
      361 gtttcaaatt gtccatgtca ctatcaaaga gtgcaaattc aaatttaact tcttgatctt
      421 cgaaagttgc actagtgtcg aaataataaa ttttattatc ttcttcgata actacatctt
      481 ttaaaataaa tgttatcgat gttttgctat gtaaataaga tgcatctttt aatatcactt
      541 tagtaggctt catcgtcaaa ttcctttcat cattcatttt ttcatgttgc ataatcacca
      601 ttactattga taattctagg atagatactc attgtataat aattattcac tagaacaaaa
      661 aaacacacat caatttggtt gtttccatcc aaaataatgt gtgtgtttat acatatatga
      721 ttaattacta aacttcatcg tttatcaata ttaaatccat tgatgcgatg ttcagagtta
      781 acgagatttc ttctgtctat ttgctctgtc tattaatact tgtttcgctt gtatttccgc
      841 ttcattatct atttctttag gctcaaacgg aataccctta cgttcacaag ctttttctaa
      901 cagataatca gtgatttcgt aatttttagg taaaattggt ccatgtaaat aagtacctaa
      961 taaattttta taatgaatgc cttctttttt atcttcatca ttattaccat aaccaaaagt
     1021 aacatgacca agtgtaccga aatcatgata tgttctacca ccgtgatttt caaaacctac
     1081 aatagttcca aaagtatcac tttcgataac aatatctcct gttaatcggt ttgtctttga
     1141 ttcagtataa aaatctaaaa tacctaaccc ttctaattct gtaccatcag gcgtgatata
     1201 ttttttccct aaaaattgat agcctccaca aatcgttaat cccggcatac catcttcaat
     1261 cgcttcttta agtggtgtct taattttact taattctttt gttgctaatg cttgttctct
     1321 atcacttcca ccaccgataa agaaaatatc acattcatca aaggtaatac cttctgtttc
     1381 attgatttct acgacattaa ctttaatatt tcgtttttta gcacgttgtc ttaaagcaat
     1441 aatatttcct atatcactgt ataaattcaa tttatctgac ataaaatgat aaatagtcaa
     1501 ttcatgcata ttatgattga cctccttcaa acgaacggtt taattgttca agcataggcg
     1561 ctaatgatgt atagtttggt attgcaactg tgaaaccttt ataatccata gtctttgccg
     1621 ttgctttata aatatcacgc tcaactataa ttggtacttc aacctctgct aacttcaatc
     1681 gcaattgaag ttcttctgct cgtgtacctg tcacgatgat agcttcaatt tgttgcttag
     1741 ataatttttc aaaatctgca tcataaatcc atgaagtatc tcgaccatct gcagcgttat
     1801 catttagcga aataacatac actttttcgc cttctaattg ttcaccaact gataaacttg
     1861 cattcattcc tgcaggattt ttagctaaat tgatcatcgc ttcttttcgt tcttttttaa
     1921 agtactgcat acgaccattg tctgatgtat acgtttcaaa gccattttta attgtttgtt
     1981 catttaaccc tagctctctt aaaacagtat atgctgctaa cgcgttataa gcgttaaagt
     2041 cacctgcaat tttcatatca tatttttcat cattgatatt taaatataaa aacggtgcca
     2101 catcaaaact tgatatttca tattttgctt gctctcgttt gaaaccacac tgacagtgat
     2161 aatgaccaat ttgattataa tgaatataat cgtattgcaa taagcgacca cagtttggac
     2221 aatatctact ttcattcatc gtactttgtt caaattcatg ggcatgtgct ttcataccat
     2281 agtacacaat cgtatcactt gcgattttca aacgactcac aaatggatca tcagcattta
     2341 gcaataattt gatgccttta ttactaattg tctctgcaat gttattaacc ataatatcaa
     2401 tttcaccgaa gcgatccatt tgatctctaa agaaattagt aaataccatc attgaaggtg
     2461 taacttcttt taacacacgt ggaatcgaac cttcatcaat ttcgattacc gcaattttag
     2521 tcttaggtgt tgattgcatg atgaatgcag aagttatacc tgcagccata ttagcacctt
     2581 cattattgtg tataatttga atattatttg cttttaaagt atgtccaatt aagtttgaag
     2641 tcgttgtttt accatttgtt ccactgataa atacaatatc atcaacttgc tctgctaatt
     2701 ttcttaatat atctgtatcc acttttctag cgatttgtcc aggtaaatct gttcctcttt
     2761 tacctactgc tctacttgct ttacgcgcca atttcgctag atggattgcc gtccactgtc
     2821 tcatgtgttt cctcctcaaa tttccactcg catcattata acatgacaag gcaacttcaa
     2881 aaaagtttct caatcacaaa ttgatacagt gtttctaaat tataatcatt ctcatttaga
     2941 taattaaaaa tgatatttat tctcaattaa atatcgctaa atgatacatt ttctcaatta
     3001 gagtgctaaa ataaggcatt aaattataat tattattatt tacaattgag aatgagtttg
     3061 ttatactaag tttaattcat agtaatttta atttacaaat aagaggtgta tcaaaatgtt
     3121 aagtaaaaat ttattagaag cattaaatga tcaaatgaac catgagtact ttgcagcaca
     3181 cgcatatatg gcaatggcag catactgtga taaagaatcg tacgaaggat ttgcaaactt
     3241 cttcattcaa caagctaaag aagaacgttt ccatggacaa aagatttata actatattaa
     3301 cgacagaggt gcacatgcag aattcagagc agtttcagca ccaaaaattg acttttcaag
     3361 catactagaa actttcaaag acagcttatc tcaagaacaa gaagtaacaa gacgtttcta
     3421 taacttatct gaaatcgctc gtcaagataa agattatgca actatctcat tcttaaactg
     3481 gttcttagat gaacaagtcg aagaagaatc aatgtttgaa actcacatca attatttaac
     3541 tcgtatcggc gatgacagca atgcattata tctttacgaa aaagaacttg gcgctcgtac
     3601 attcgacgaa gaataattaa acatcactac aatagacaga taaatatcat acgacatgat
     3661 aggcatttgg gtcacttaca ataacccaat gtctatatta ttttgcttta cggagatcac
     3721 tagattcatt ttctgaatca ttgatctgcg ttttttcatt ttcaaggcta attattgtat
     3781 ttttagtcat ttatttttta aactactaat gttaataact ctaaatttga tgttgaatta
     3841 atttgacgat tttaaagcat atcatcattt actttttaat cagagttaca tccaaatgat
     3901 agatttcacg ttataccttc acgtataata ttatgtatcg tttgtaagca aatgactaaa
     3961 agtctattaa tatatacatt taattaattg aaaggattga ctacatgata caagatgcgt
     4021 ttgttgcact tgattttgaa acagcaaatg gtaaacgtac aagtatttgt tctgtcggaa
     4081 tggttaaagt cattgatagt caaataacag aaacatttca tactcttgtg aatccgcaag
     4141 actatttttc acaacaaaat attaaaattc atggcataca accagaagat gttgaaaatg
     4201 cacctacgtt tgactacgta tttccatata tgatgcaatt tattgcagat ttacctgttg
     4261 tcgcacataa cgcggcattt gatatgaacg tcttacatca aagcattcaa aatattggtt
     4321 taccaactcc aaatttaact tacttttgta gttatcaact tgctaaaaga accgttgatt
     4381 cgtatcgata cggtttaaaa catatgatgg agttttatca attagatttt catggtcatc
     4441 atgatgcatt gaatgatgcc aaagcatgcg caatgattac ttttaggcta ctgaaaaatt
     4501 atgaaaattt aacatatgta actaatattt atggtaaaaa tctaaaagat aaaggctagg
     4561 actaaataaa atactccctt caaaagtaag cattgtaaaa atgtaaactt tgcagggagc
     4621 tttattttat ataaagtcat atatcgtcat atttttataa gttgattgtt ctaaattacc
     4681 tacagtgaca ccaataagtc gaattggtac atcagggtct tttaaatcgt tataaagtaa
     4741 atatgcaata ttataaatat cttcttcaga actaaccgaa tctcttaaac tcatctgttt
     4801 agatagcgtt tcaaattgat aagttttaat tttaaccgtt acagttttag ctgacttctg
     4861 taatttattt agacgttcag ctgttttacc tgacaattcc catacttttc ttaaaatctc
     4921 ttcatcatca ttcacgtctg ttgcaaatgt gcgttcagtc cctactgatt ttcttactct
     4981 tgatgatttc acttcactat ggtcaatacc gcgtgccttg ttatataaac cccgacctct
     5041 ttttccaaac aaacgtatta attcaaattc cgttttctca tataaatctc taccgttaaa
     5101 aataccatta tcatgcatta cttttttgga agctttacct acgcctggaa aatctccaat
     5161 atccaatgtc atcaaaatat catggacatt ttgataatca atcacagtca taccatcagg
     5221 tttattcata ccactcgcta atttagctaa aaatttgtta taagaaacac ctgcagatgc
     5281 tgttaaatgt gtctgctcta gaatatcttt tctaatatac tgagcaattt tcgaagcagg
     5341 aaggtctggt ctcactaatt ctgtaatatc taaatacgct tcatccaatg acatcggttc
     5401 taccttatct gtataacttc ggaaaataga cataatctgc gcagatgttt ctcggtaagc
     5461 accaaaatta cttgtgacaa agtatccatt tggacataat ttatgcgctt gtgacatagg
     5521 cattgctgaa tggacgccgt attttcgtgc ttcataggat gccgtagaga caacacccct
     5581 actgcttgct ttaccaccaa caatgactgg tttccctttc aatttggggt tatctctcat
     5641 ttcgacttgt gcaaaaaaat agtccatatc tatatgaata attcgtctct cagtcaagtg
     5701 ctcacctccc tactaatttt tacttttata acgcacaaaa atatctcaac ataattatac
     5761 gctgtgtacg atttttttac ataaatcttg cacttagcga taactatatt gagataacta
     5821 caagttgtta taaaatcaat tgctatttaa gcatgatgat gaagacgatt gagtaagaaa
     5881 acataggtaa tctgaaataa ttcaagcaaa ttcattttgt tggtatcatc atattaaaat
     5941 ttattattga gtcggctttt gatgatacaa ataaatacta tcttcatctt gttgaacata
     6001 tttaaaacta ctagatattg ttaatgcaac aagcagaata tctataaagc aataagcggt
     6061 atgcatacta aacataaaaa taagtgatga ataaccaaat accttaatta aaataagcaa
     6121 gccagtactt aataggatta gtggtgacag cataataatt gagaattgcc atttgttgaa
     6181 gcaagcatct gctgtttgga ataagattct gtcttttttt atattaaaca taggtttgct
     6241 atctttttta aataaaagaa ataatgctct atggataagt tcatgtaaaa tcaataaaat
     6301 aatgaatcca gcaaacccat atacaagatt gatgatgata ttttgatcga caaccgctgt
     6361 gacacctaac gcccacttat acgtaaataa aatcacgaat aacgcaataa caagttgcaa
     6421 gataataaac cttcgcattt gaaaattatt tgtcgttaaa tcaattttat gcattaccaa
     6481 ccctcccgat catgacattc ttattcttct ttaaatatag tatacaatgt cacatttaat
     6541 ttaaaaagtt catatcaaga aagtaaattg gctgtaataa aattttaata tacgacttct
     6601 ttcttcactt attaaggcga aattttatct caaatcatgt gcgctatttc aaattgaata
     6661 atgccactgt ctcaacatgt gttgtttgtg gaaacatatc taccggtgtt acctcttcaa
     6721 gttgatattt ttcagctaat aataatgcat cacgttgctg tgttgcggga ttacatgaaa
     6781 tatagacaat acgcttaggt tctaatgtaa gcaaagtctg aataaacgtt tcgtcacagc
     6841 cctttcttgg cggatcaacc attacaacat ctggtttaat cccttgtgct ttccattgta
     6901 aaataacttc ttcagctttc ccacagacaa aagttgtatt attgcattgg tttatagtcg
     6961 cattttgttg tgcgtcttca attgcagaag gtactacttc aacaccgtat acatgttttg
     7021 caagtggtgc catatatagc cctattgttc caataccaca ataggtatct aatacaactt
     7081 cattacctgt caattgcgca tactcaattg ctttattata taatttctct gtttgttcag
     7141 aattaatttg gtagaatgac tgatcactta ttttaaatgt actatctgtt aattgatcaa
     7201 taattgtatc tttaccatat agcgttatag attgacgtcc cataataaca ttagagtggc
     7261 tatcattaat gttttgttta atgcttgtca cattaggaaa tgcatctaat atcttctcaa
     7321 caacagcatt tttttgtggc cactttttac cattagttac aaaaataatc atcatttcgt
     7381 ctgtatgata tcctgttctt acaaccaaat gtctcattaa accttttttc aattgttctt
     7441 gataaatact tacatttaaa tcttttaaaa tagatttaac ttcattcatc acttcttgat
     7501 gttgtgaatc ttgtattaaa caactttcca tgtcaataat gtcatggctt ctttgacgat
     7561 aaaagcccat aataacttca ttctgttcat tcttaccaac tggaatctgg gacttgtttc
     7621 gatatctcca aggatctgtc atgccaactg tatcgttaat cttagaatta tcaaaatgcg
     7681 cttttcgctg aaacaaatta atcacttgtt cctttttcat ttcaagttgt gcttcgtatg
     7741 ataagtgttg aagttggcac ccaccacaac gttcataata tatacatggc ggtgcaacac
     7801 gattctcact tctcgtattt atattaagca acttacctat cgcaaagttc ttttttactt
     7861 tgataatttt atattcaatt tgttcattaa ttaaagcttg tggtatgaaa ataggaaagc
     7921 gatctatttt tacgacacca tggccttcat gcgttaaatc aacaactgtt cccgttttta
     7981 tgtcattttt agctattgct tgcaaaattt tacctccaaa atgaacaggt taggaacaaa
     8041 attatgcgct tcctaacctg ccattatata tttcactatt tctgtttatt cttcgattaa
     8101 attgtcatca acatgatcat tatttattaa ctcttcattt acaatatcat taggtgcaaa
     8161 gacatctata tgacgttcta gatttaagaa attcgctggt aatttaccac catattctcc
     8221 atctacattt agttgtaagt ctgtgaatga tgaaatatta attgcctttg ctttttcata
     8281 aataacttta ggatgcttag tatgttctcc tcttgaagct aaagtcataa tatgaccaag
     8341 ttctgcaagg tttgattttt caactataat taacgtaaaa tagccgtcat ctaacttagc
     8401 gtccggcact aatttttcaa atcctgccat tgaatttgtt aaacctaaaa agaataataa
     8461 tgcttctcct tggaaaacat taccatcata ttcaattctt aaatctacag ctttcatttg
     8521 aggtaacatt tcgaaacctt tgatgtaata agcaaatgga ccaacaatag atttcaattt
     8581 actcggtgtt tcataagaga cttgcgtcaa ttgtccgcct gcagctaaat taataaagta
     8641 tcgattattc attttaccaa tatctacttt agtagaatga ccttcaatga tgacatcaag
     8701 tgcccccatg atgtcattag gtatatgcaa tgcacgtcca aagtcattaa cagtacccat
     8761 aggaatgaca cctagcttag gacgattagg cttttctgcg ataccattaa ctacttcatt
     8821 taatgttcca tcaccacctg cagcgattaa tacatcataa ttttcatgca tagctctttc
     8881 tgcttcaagt gtggcatcac ctattttctc ggttgcatat gcactcgttt catatcccgc
     8941 tttttctaat tttattaagg catcaggtaa ttctctttta aatagctctt tacctgatgt
     9001 cgggttataa atgattctag cacgtttcct catatcttat ccctctactt aaaattcata
     9061 tattttaact tcatctttgt ttcgtctaat agggagtggg acagaaataa tatttaacaa
     9121 aatttatttc gttctacccc aacttgcatt gtctgtagaa tttcctttcg aaattctcta
     9181 tgttggggcc ccaccccaac ttgcacatta ttgtaagctg acagaaagtc agcttctttg
     9241 tttgggggcc ccgccaactt gcacattatt gtaagctgac agaaagtcag cttctatgtt
     9301 ggggccccac tagaattgaa aaaagcttgt tacaagcgta ttttctttca gtcaactaca
     9361 gccaatataa cattgtagtg cctaggacat tgaatttatg acccaggctc agtcttattt
     9421 catcattctt aatatcgtta aagaccaact tgtatcttaa acaaatacta tctcaatatg
     9481 tacaaagctt gttatttatt cagcattttt tgccgttctt cattatatag acttcgtcag
     9541 ttatgctatt ttacctttaa aatgatgttg taaatataat gttgtcttta tacaaaaatg
     9601 ttttatacct ttttattata cagtttgcct taatataatt aaagctaacg cattaataaa
     9661 attaatattt ttaccattaa catgtacaat gaataaagtt aaaagtaatt tgacttctat
     9721 agatataaat aaaccctcga ttgcatctaa gtcagcaatc aagggtttat tttttaaatc
     9781 ttcatagttt gatgatttaa attatctttt atctaattct tgttttaata gttgatttac
     9841 taattgtgga ttagcttgac ctttagacgc tttcataatt tgaccaacta agaagcccat
     9901 agctttgcct ttaccatttt tgtaatcttc aactgattgt tcgttattgt ctaatgcttc
     9961 atttacaaat tttagaagtg ttgcttcatc agaaatttga actaagccat tatcttccat
    10021 aatctgttta gcattaccac ctttagctgc taactctggg aagactttct tcgcaatttt
    10081 actgctcatt gttccgtctt cgataagttt aatcatacct gctaaatttt ctggtgttaa
    10141 tttagtatct aataattcta cttgattttt atttaaatat tcgtttacgc cacccattaa
    10201 ccagttagat gttaatttaa catctgcacc gtgttcaatt gttgattcaa agaaatctga
    10261 catttcttta gtcaatgtta atacgtgtgc atcgtatgca ggtaaaccta attcatttac
    10321 atacttagct ttacgttcat ctggtaattc aggaattgtc tgacgaacac gctctttcca
    10381 agcatcatca atatataaag gtacaatgtc aggctctggg aagtaacggt aatcatcaga
    10441 accttcttta acacgcatta aaattgtttt acctgtagat tcatcaaatc gacgtgtttc
    10501 ttgtccgatt tctccaccat ttaacaattc ttcttcttgg cgtttttctt catattctaa
    10561 acctttacgt acatagttaa atgagtttaa gtttttcaat tcggctttag taccaaattt
    10621 ttcttgacca tatggacgta aagagatgtt agcatcacaa cgtaaagatc cctcttccat
    10681 cttaacgtct gatacaccag tgtattgaat aattgaacgc aatttttcta aatatgcata
    10741 tgcttcttta ggtgaacgaa tatctggttc agatacgatt tcaattagcg gtgtaccttg
    10801 acggttcaag tcaactaatg aatactcacc tttatgtgtt gacttaccag catcttcttc
    10861 catgtgaaga cgagtaatac cgattcgttt tgtttcaccg tcgacttcga tatcgatata
    10921 tccattttca ccaattggtt gatcaaattg agaaatttga tatgcttttg gattatctgg
    10981 atagaaatag ttcttacggt caaacttaga ttctgttgcg atttccatat ttagtgccat
    11041 tgcagcacgc attgcccagt ctactgcacg cttattaaca actggtaaga cacctggata
    11101 tgctaagtcg ataacatttg tatttgagtt aggttctgct ccaaaatgcg ctggtgatgg
    11161 agaaaacatt tttgagtccg tttttaactc tacgtgaact tcaagtccta taactgtttc
    11221 aaaatgcatg atttccactc cttataattt ttcataaacg tcatgtaaat tgtattgtgt
    11281 ttcatattga taagcgacac gatataacgt tttttcatcg aatggtttac caatgaactg
    11341 taaaccgatt ggtcggccat ttgattgtcc acaaggaaca gaaataccag gtaatccagc
    11401 taagtttact ggtgttgtta ataaatcatt ggcatacatt gttaatggat catcaatttc
    11461 ttcacctaaa ttaaacgcag ttgtaggcgc tgttggacca actactacat cataattttc
    11521 gaatacttta tcaaagtcat ttttaatcaa tgttctaact ttttgagatt ttttatagta
    11581 agcatcatag taacctgaac ttaatgcaaa tgtacctaag aaaatacgac gttttacttc
    11641 tttaccgaaa ccttcagatc ttgacatttt atataattct tctaatgaat gagcttcttt
    11701 agaatgataa ccataacgaa ttccgtcaaa acgagaaagg tttgacgaag cttctgatga
    11761 tgcaatcacg taatatgatg gaataccaaa tttagtattt ggcaatgata cttcctcaac
    11821 gacagcacct aaagatttta aagtttctac agcgttttga actgcttctt ttacgtcatc
    11881 agctacacct tcacctaagt attctttagg taatgcaact tttaatcctt taatatcttt
    11941 accaatttca gatgtaaagt ctacatcatc aactggtgca cttgtagagt cattaacatc
    12001 tgcaccagaa atagcttcta atacgattgc attatctttt acatttcgag tcaatggacc
    12061 aatttggtct aatgaagatg caaaagcaac taatccaaat cgagatacac gaccgtatgt
    12121 tggtttcata ccgacaacgc cacaatatgc agccggttgt ctaattgaac cacctgtgtc
    12181 tgaacctaag ctaaatggta ctaagccagc tgcaactgct gctgcagatc cacctgatga
    12241 accacctggc actgctttat ggtcaaatgg gttaactgtt tttttgaaat aagatgtttc
    12301 tgttgaacca cccattgcaa actcatccat atttaattta ccgattaaaa cggcattttc
    12361 attatgtagt ttttccatta cagtagattc gtaaattggc acaaaacctt ctaacatttt
    12421 acttgcacat gttgtttcta atccgtttgt aataatgtta tcttttatac ccattggaat
    12481 accaaataat ttgccatcca tttgatcttt tgcttgtaat tcatccaatt cttgcgcttt
    12541 tttgattgca ttttctttat ccagcgctag aaaagactta attgttggat cagtctcttc
    12601 aattgcatca tatatatctt taacaacatc agatggtttg atttttttgt cttttattaa
    12661 agttaataaa ttctcaaccg attcgtagcg aatgctcatc ttacgcgtcc tcctcattca
    12721 tgattgtagg cactttaaat tgtccatctt ctgtttcttt ggcatttttc aaagctaatt
    12781 cttgtggaat acctttaatt gctttatctt cacgtaaaac gttttgtaaa tctaaaacgt
    12841 gatatgtagg ttcaacgcct tctgtatcag cgctatcatt ttgttttgca aaatctaaaa
    12901 tgctttctaa tgtgttggcc atttcttccg tttcttcagg agaaatttga agtcttgcaa
    12961 gattcgcgat atgctcaact tcttcacgtg ttacttttgt cattaataaa agcctccttt
    13021 aagtcattca tcactaaatt gtatcaaatt tccaattaaa aatctaagta tttatgaggt
    13081 gctactttaa tttcatataa actgtataaa cattatcatt cgtttatcaa atcatttttt
    13141 atgaaaacaa cactctttta atattagaca acccaattca atattatgat tatgcaattt
    13201 taattataaa aatttgctca tatccaaaaa atacgaaagc gctttctata ttggtatgca
    13261 agtatttcaa aaagaataaa tttaattttc ctacttttct aaacatttat ctttatgtat
    13321 aatgttttca agtaactaaa ttataaatta aataaaggga gtgtttatca tgcttacaat
    13381 ggggacagca ttaagtcaac aagtagatgc caattggcaa acttatatta tgattgccgt
    13441 ctacttcttg atactaatcg ttattggctt ttacggttac aagcaagcaa ctggtaacct
    13501 aagcgagtac atgttaggtg gacgtagtat tggaccgtat attactgcat tatcagctgg
    13561 agcttcagat atgagtggat ggatgattat ggggctacct ggttctgtct atagcactgg
    13621 tctatcagct atgtggatta caatcggttt aacattaggt gcttatataa attactttgt
    13681 tgttgctcct agacttcgtg tttataccga attagctgga gatgcaatta cattaccaga
    13741 tttctttaaa aatcgtttaa acgataaaaa taatgtgtta aagattattt ctggattgat
    13801 tatcgtagta ttctttacat tatatacaca ttctggtttc gtatctggtg gtaaactatt
    13861 tgaaagtgct tttggattag attatcattt cggtttaata ttagttgctt tcattgtcat
    13921 tttctatact ttctttggtg gatatttagc tgtatcaatt acagatttct tccaaggtgt
    13981 cattatgtta attgcgatgg ttatggtccc tattgttgct atgatgaatt taaacggctg
    14041 gggaacgttt catgatgtag cagctatgaa acctacaaat ttaaatttat ttaaagggtt
    14101 atcatttata ggaattatct ctctattttc atggggatta ggttatttcg gtcaacctca
    14161 tatcattata aggtttatgt ctattaaatc acacaagatg ctacctaaag ctagacgttt
    14221 aggtattagc tggatggctg ttggtttatt aggcgctgtg gctgttggtt taacaggtat
    14281 tgcattcgta cctgcttatc atattaaact agaagatcct gagacattat tcatcgtgat
    14341 gagtcaagta ctcttccatc ctcttgtagg tggtttctta cttgctgcga ttctagctgc
    14401 aattatgagc acgatttctt cacaattact tgtaacatct agttcactaa cggaagactt
    14461 ttataaatta attcgtggtg aagaaaaagc taaaacgcac caaaaagaat ttgttatgat
    14521 tggaagatta tctgtattag ttgtagcaat tgttgccatc gcgattgcat ggaatccaaa
    14581 cgacacaatt ctaaacttag taggtaacgc ttgggccgga tttggtgcat cgttcagtcc
    14641 acttgtgcta tttgcacttt actggaaagg tttgacacgt gccggtgctg taagtggaat
    14701 ggtttcaggt gccttagtcg ttatcgtttg gattgcgtgg attaaaccat tggcacatat
    14761 caacgaaata ttcggcttat atgaaattat tcctggattt attgtaagtg taatcgttac
    14821 atatgttgta agtaaactta ctaaaaaacc tggtgcattt gttgaaactg acttaaacaa
    14881 agttcgtgac atcgttagag aaaaataatt cataagtctt aacaaattaa aaaggtacta
    14941 atgttaatca aaattatgac taacattggt acctttttat tatctttaat ttacaattaa
    15001 ttactgtaaa tatgaacttg cggttctttg tcatcttttg tcttactaat taaagcacgt
    15061 ggttggttac catctttaat acgaatttca tagttatcga ttttatcgaa atatttattc
    15121 gcttgttcag taacgtactg tgtaatacca attgtttcag cttgtccata gtaatcgatt
    15181 ggtaaatcta ctactaatcg ttgtggcttt ttatcaacaa atttaacttt ccctactgct
    15241 tgtgtgaaat tagaaaaata tgattgcaaa ttatcattaa attgcttgaa attattattt
    15301 aaattttcat cataatctgc tgctgttgaa gaaggtaata aagctgattt ttcattgata
    15361 ttatgccatt cattaagctt tgtttgactc ttttctgcag tcgcttgagt gataaattca
    15421 cctggtgtga ttgaatcttc acttgattgc ttataaattg caaaatgaat tggtatatct
    15481 tttaaatcat cattttcacg taaccttgat aatatctcac tagccatttg tttaccttgc
    15541 tttttaactt cgctatcatc tagtttttta ctaaaagtcg atccatcttt ttctttttta
    15601 tagtaataaa cactattcat agctaaacca atcgtcatac ctttaatatt cttacctttt
    15661 gtatctccac caccataaaa atcttgctct aaaatgttag ataaataggc tggtgatttt
    15721 tctgcaatct tttcaggatc tgtttcacct tcgtgtgatg gattaagtcc taaattttca
    15781 ttcgctttct tgtctttttt atctttttca gacattttat cgatttcacg ttttgtatac
    15841 ttaggattta aataggcatt aattgttttc ttgtccaaaa attgaccatc ttgatacaaa
    15901 tatttatctg ttggaaatac ttctttactt aagttcaata aaccatcttc aaagtcgccg
    15961 ccattataac tatttgccat gttatcttgt aaaagtcctc ttgcctggct ttctttaaat
    16021 ggtaacaatg tacgatagtt atcaccttgt acatttttat ccgttgcaat ttcttttact
    16081 tgatttgaac tattgttatg tttttgatta tcttttccag cctggtcatc cttatggtta
    16141 ccacaagcag cgagtataaa gatagctgta atcaataata ctaatgtacg cttcatcgac
    16201 atacccctct aactatttaa ttcattttgc ttatctacaa attgttgctc tgtccaaatt
    16261 tcaataccta aactttgtgc ttttgttaat tttgaacctg catcttcacc agcaataacg
    16321 acatctgtat ttttagtaac gctacttgta actttagcac cttgtgatgc aagccattta
    16381 gatgcttcat tgcgtgtcat ttgatgtagc ttaccagtca gtactatcgt tttaccacta
    16441 aattcaggat gtccttcaat atctgatgtt ttgatacctt tataaatcat attaacatgt
    16501 ttatctttta atttttgaat taaagcacga atatcttcat tttctaaata agtaactaca
    16561 gattgtgcta ctttatcacc tatatcatga atttctacta attccgcttc agttaccgtt
    16621 agtaatcgat ctatcgtttc atatttttct gctaacactt ggctcgcttt aacacctaaa
    16681 tgcctaatac ctagaccaaa taataaattt tctaaagagt tgtccttagc ttgttgaatg
    16741 gcagctaata aattatcaac ttttttctgc cccattctgt ctaaaggtaa taaatcttct
    16801 tctgttaaat agaaaatatc agcaacatct ttaattaatt cgctttgata aagctgttga
    16861 ataattttag tgcctaaacc atcaatattc atggcttgtc ttgatacaaa gtgaatcaat
    16921 ccttcaacaa gttgtgcttg gcattttgga ttaatgcaac gaagtgctac ttcgccttca
    16981 atacgtacta attcatgtcc acaacttgga caatgggttg gcatatgata tgtgacagca
    17041 tcctcaggtc tacgttctgg aatactacgt acaacttcag gtatgatgtc acctgctttt
    17101 ttcactacaa cactatcacc aattcgaata tctctgtcat gaattaaatc ctcattgtgc
    17161 aaagatgctc ttgatacagt tgtaccagct acttttactg gttctaaaat agcagtaggt
    17221 gtgactacac ctgttcgtcc aatacttaat tcaatatcta ataatttagt tactacttcc
    17281 tcagctggaa atttataagc aatggcccat ctaggagatt tttgtgtgaa tcccatctca
    17341 tcctgttgat ctaaatcatt aaccttaata acaatcccat caatatcata aggtaatgac
    17401 tctctttggc ttgtccattt ttcaatatac tctaaaacac catcgatatt atttacacgc
    17461 gctctatttt tattcgttgt aaaacctaat ttatctaact catctaatgc ttcactttgc
    17521 gaacgcgcat tgaaatcagt gaaatcattg acactatata taaatacgct tagctttcgt
    17581 tttgccgtta atttagaatc taactgtctt aatgatcccg cagcagcgtt tcttggattt
    17641 gcaaataact gctcatcatt tttttctttt tcttcattta atcgtaaaaa tgaacgtctc
    17701 ggcatatatg cttcaccacg aacttctaca tttaatggtt ctttcatttt caaaggtatc
    17761 gcatgaattg tttttaaatt ttcggtaata tcttcacctg ttgttccatc accacgtgtt
    17821 aaaccttgaa cgaagtatcc atcaacatat ttcaatgata ctgctaagcc atcaattttt
    17881 aattcgcaca tatattcaac gttgccaatt tgttcacgta tgcgttggtc gaattttctc
    17941 aaatcatcct cattaaatgc attccctaaa cttaacattg gcgtgtcatg gttgacttta
    18001 ttgaaagagg cttgggcttc accgccaact ctaactgttg gagaatctac agtcttatac
    18061 tcaggatgct cctcttctat tttaatcagt tcatgaagta atttgtcata ttcactatct
    18121 ggtacagatg gattatcctc tacatagtat tcataactgt attgatttaa taaatcatgt
    18181 aactcgttca cacgagacga taaatcagcc atcccttaat cctccttttt ttcaattggt
    18241 gcaaattgcg ctaacaaacg ttttggccct tgtgatttaa agataatatc tagttcgatt
    18301 gagccatttt tctcgtttac attactcacc atgccttctc cccaggcttt atgcatcact
    18361 ttgtcaccta cattccaatc agatgacaat acttgttttt tcgttgacgt tgttcgttga
    18421 ctaaatccgc gtttagcaaa aggttttgcc ttaggttgta tcgtttgtcg tttgccactt
    18481 gaatgatttt ctaatagtga ttctggaatt tcctttaaaa atctggatgg catatttgac
    18541 tgagggcgac caaataacat tcttgatgtc gcatgagtga tatataacac ctcttcagcc
    18601 cttgtaattg ctacataaca aatacgacgt tcttcttgca tttcatgatc atcttcactc
    18661 ttaatcgctc taatatgtgg gaataaagat tcttccatcc ccattataaa gacaattgga
    18721 aattcaaggc ccttagccga gtgcatcgtc attagtgtta cgccattttc agtatctgcc
    18781 tcatcaatat cagctactaa cgataaatcc gttaaaaagt taattaatga ctgttcttct
    18841 aatggggtat tttcctcata gtcttttggt actgacataa attcatcgat gttttctaat
    18901 ctacttcgag attctaatgt attttcacgt tcaagcattt ctcgatagcc agacttttgt
    18961 aatacttcat caactatttc atgaatttct aaaaattctt gttcttttat caagctttgg
    19021 attaactcgt aaaaattaag acactcttgt gtcacctttt ttgacaatcc gataaaatca
    19081 gcttctccaa gtgcatcaaa catactgata ttgttttgaa gtgcatagtt ttgaactttt
    19141 tcaacagatg aaggacctac acctcttttt ggaacattaa taatacgttg caaactaatg
    19201 tcatcattac tattggcaat tatacgcaaa taacttaata aatctttgat ttctttacgg
    19261 tcatagaact tttggccacc aaccattgtg tatggcatat tagatttcat gaatgtttcc
    19321 tcaagtacac gtgattgtgc attcgttcta tataaaattg ccatatcttg atatttctta
    19381 ccattacgtt gatgcttcat aatttctcgt attacaaatt ccgcttcatc acgttccgtc
    19441 attgcttcat agtaatgaat tttctcacca ttcgtatttg cagtccacag tccttttggc
    19501 ttacgttcag aattattttt aatcacttcg ttagccgcat ttaaaatcgt tttcgtcgaa
    19561 cgataatttt gctctaaaaa gattgtattc gcttctggat agtctttttc aaatgataag
    19621 atattttgaa tatcagcacc acgccaacca taaattgact gatctgagtc acctacaaca
    19681 cataagtttt taaacttact tgctaataat ttaactaatg tgtattgtgc tttattagta
    19741 tcttgatact catctacatg aatatattgg aatttgttct gataatattc tagaacttct
    19801 ggtacacgct caaataagtt aatcgttgtc ataataaggt catcaaaatc taacgcttca
    19861 ttacgtgaca attggcgttg atatccacta taaaccgttg ctaccatttg cgagtgataa
    19921 tctgtggctt ctttttgagc atctgcaggt gttttaagtt catttttcaa attactgatc
    19981 gcaccgataa acatacgagg ttcaaacttt ttactatcaa tattttcatt ttttaagacg
    20041 tctttaataa cagatttttg gtctgtagga tcaattatcg taaaattgcg ttctatacca
    20101 attcgatctg catcacgacg taaaatacga acacacattg agtggaatgt tgacatccaa
    20161 ataacttctg cttgatcacc tactaatttt tgaacacgtt ctttcatttc tcttgcagct
    20221 ttatttgtaa aagtaatagc caaaacattg tatggtgaga catctttttc gtctaataaa
    20281 taagcaattc tatgtgttaa aacacgtgtc ttccctgaac cagcacctgc cataattaac
    20341 aatggtcctt ctgttgtctt tacagcttca ctttgctctg tattcatatg atttaataac
    20401 gcattcattt actagactcc tttattttta ctgtttttaa agctttttta atatctttat
    20461 aaataatatc accgacgata attgtatctg caatagctgc catctctgtc gcttgttgtt
    20521 ctgaggaaat accgccacca taaaaaagtt gcgtttctgt tagatgttct gagacagctt
    20581 gaacctttga tacgtcgcca taaatgccac tatactctat atacataacc ggtaatcgat
    20641 acatatgatt gaccatttgg gcatatgctt ctaaatcttc tgttgttaaa tctgtatttg
    20701 ctttggtatg ttttgccatt ttgctatcag cattgcacac gacataccct tcaaatatta
    20761 cttcctcaaa atctatacta tgtccatatg ttttaagcgc ttctaataat gtaccattgt
    20821 gaaatacaac atctgtactg ttcagtactg taggtacaaa ataaaaatca aaaccaggca
    20881 ttacactttc gatgtttgat atttcaagta ctaaaggcaa tgggtatctt cttactctgc
    20941 tcattaaatg aatgacatta tcttcagtaa cgtcatcagt tccaccaatc ataattgcat
    21001 ctgtttgaga catacaaatc gcatctaaat catcatctga aatatgttta gctgggtcta
    21061 atttaaaaat atggcgccat tttttaatgt catacatttt ccgaaactcc ttttttaaca
    21121 tacatatgat tatagcattt ttaagtgtcg agttctaaag acttaatatg aaatttaagt
    21181 tcttttactc tgtaaatttt ttcgtgaaaa aacaatgatt atctacctta ttagtgcaga
    21241 tagataacca ttgtttatcg ttcatttatt attcatttgt ttcaatattc atacgatcta
    21301 aaatcattgt ataagcatca ttaccccatt gtaatgaacg cttcactcta gaaatcgttg
    21361 cagtcgatgc tccagattct tgttcaatcg ttgcataggt ataaccttgc ttaatcattt
    21421 tagcaacttg taatctttga gacagtgatt gaatttcatt tacagtacac aaatcatcga
    21481 aaaattggta acattcttct ctattttcta acgttagtat tgcatcaaac aattcatcta
    21541 acgctgcacc tcgtaatttt tcaatttgca tatcgaaaca acccctataa ttttattctt
    21601 tatgtcattt taacgcatta atctattaaa gtgtagcgat ttatatttta ttaaatctga
    21661 atcgttactt tatttaattt tatgctaatc cagcgcgttc gaaaatagtg tcaacttgat
    21721 tcaaatgatg tttaggatcg aaacattcat ccaattcttc ttttgttaaa acacttgtaa
    21781 tagactcatc ttgttcgatt aattcacgga acggtgtttt cgtttcccaa gatatcatcg
    21841 cttttggttg tactttgtcg tatgcttctt cacgaaccat acctttatta attaatgcta
    21901 ataagacacg ttgtgagaaa atcagaccaa atgttttatc tatgttatta cgcatattat
    21961 cttcaaatac agttaaacgg tccacaatat ttgtgaaacg attcaatgca taatctagtg
    22021 ctattgtaac atctggtaac ataatacgct cagcagaaga atgagaaata tctctttcat
    22081 gccataatgg cacattctca taagctgtag taatataacc acgaatgact cttgaaatac
    22141 ctgtgatatt ttcagaacca attggatttc gtttatgagg cattgcagat gaaccttttt
    22201 ggccttttgc aaatgcttct tcaacttctc tcgtttcggt tttttgaagg ttacgtattt
    22261 caacggcaaa tttttctagt gatgtcgcga ttaatgctaa tgtcgcaata tagtatgcat
    22321 gtcgatcgcg ttgcaatgtt tgcgttgata caggcgctgt gccaatacct aaatgtttac
    22381 acacataact ttctatttca ggaggaatgt tagcaaaagt acctactgca ccactcattt
    22441 tccctacttc aatttcttct cttacttgtt tgaaacgttg taagttacgt tgcatttccg
    22501 tgtaccacaa tgccattttg acaccaaatg tagttggttc tgcatgcact ccatgtgtac
    22561 gtcccatcat caatgtatat ttataatttt ttgctttttc agctaaaacg tcgataaatc
    22621 tttctaaatc tttttcaata atgtcatttg cttgtttaat aacgaaactt aaagctgtat
    22681 ctacaacatc agtagaagtt aaaccataat gtacccactt acgttcttca cctagcgttt
    22741 cagaaacttg tctagtaaag gctacaacat catggcgcgt ttcttgttca atttcttgtg
    22801 cacgttcgac atttaccttt gcgttttgac gaattttttg tacgtcagct ttcggtatat
    22861 gtcctaattc actccatgct tcacatgcta aaatttccac ttctaaccat gcttcatagc
    22921 gattttgatc agtccaaata ttagacattt cttctctaga atagcgttca atcatttttt
    22981 taactcctat cgtatgtata ctcgaatttt caaaaacaga acatttttta ataaaaataa
    23041 acaaaaagtt cgtttttaac tctactcttc acagtttaac agaattggtt ataaatgtat
    23101 aaaaaatttc cgtatattct cattctattt tattttttac attaatacaa ttatctatgg
    23161 cagattaata ctgatttcta attgtaaatg ttcgcaatac tttaatctaa ttggtattta
    23221 taatgatcca ttgagaatat atttttgcat tttagttttt ctcccctcaa ctacactatc
    23281 caacttttta ttacaattag gacaatcaat agctttagta ataccagaat ttattcactt
    23341 tacttctcct attgtatgga attttcaaca atctactttt cctagtttca taatatttct
    23401 tcttacattt cataatatgt tttaaaatta ttcgcttcta atataacaaa ttttctactc
    23461 acaaaaagaa tatatattct attgcatagg tgtggcattt taaatataag acattaatat
    23521 tgaactccag aatcgtataa atacgaaact gattatagaa aaatacgcag agattttttt
    23581 aatagtaaat ttttatagaa agacataaat tgactacgct tctttcaaaa tcgactaata
    23641 tattttcatg aaactttcga tcatattaat tattgaatga gatgtttgca atttagtaaa
    23701 taagctacta aattattaca taaactgata atgtgtaaaa attaattatt ttttaaacat
    23761 agattaaaat tttatattga aactcttaac taaatttaat ataattattt tataataaaa
    23821 taaaggagtt tttatatgaa aagaaacttt ccaaaattaa tagctttaag tttaatattt
    23881 tctttaagtg ttacccctat tgcaaacgct gagagcaatt caaatatcaa agcaaaagat
    23941 aagaagcatg tgcaagttaa tgtcgaggac aagagtgttc ctactgacgt acgtaattta
    24001 gcgcaaaaag actatctttc ttatgttact tcacttgata aaatctataa taaagagaaa
    24061 gcctcgtata cacttggtga accttttaaa atttataaat tcaacaaaaa gagtgatggt
    24121 aattattatt ttccagtttt gaacactgaa ggtaatatcg attatatagt tacaatttca
    24181 ccaaaaatca ctaagtattc tagttcatct tctaaataca caatcaatgt ttctccattt
    24241 ttatctaaag tcttaaatca atataaagat caacaaatta ctattttgac aaactcaaaa
    24301 ggttactatg tggttactca aaatcataaa gctaaactag tactaaaaac accacgtcta
    24361 gaagataaaa agttaaaaaa gactgagagt atacctactg gcaataatgt aacgcaacta
    24421 aagcaaaaag cttctgtcac tatgccaact agtcaattta aaagtaataa ttatacttac
    24481 aacgagcaat atataaataa acttgagaat ttcaagataa gagaaactca aggtaacaat
    24541 ggttggtgtg caggctatac gatgtctgaa ttactaaatg cgacttataa tactaacaaa
    24601 tatcatgcag aagctgtgat gagattttta cacccaaact tacaaggaca acgattccaa
    24661 tttactggtt taacgccaag agagatgatt tattttggac aaactcaggg aagaagtcca
    24721 caacttctta atagaatgac tacatataat gaagttgaca atcttactaa aaataataaa
    24781 ggaattgctg ttttaggtag tagagtagaa tcaagaaatg gtatgcacgc agggcatgcc
    24841 atggcagttg ttgggaacgc taaattagat aatggacaag aggttataat tatatggaat
    24901 ccatgggata atggttttat gactcaagat gctaaaaaca atgttattcc agtatccaat
    24961 ggcgaccatt atcgatggta ttcatctatt tatggttatt aaaatttgta ttttagaaag
    25021 gtaaataata ttatggaaca aattgaatta tttagtattg ataaattcaa atgtaattca
    25081 gaggctaagt attatcttaa tattattgag ggagaatggc atcctcaaga tttaaatgat
    25141 agtcctttaa aatttattct cagtacctca gacgattctg attacatttg caaatatata
    25201 aatacagaac acaaacaact cacattatat aataaaaata atagctcaat tgttattgaa
    25261 atatttatac caaatgataa taaaatacta ctaacaatta tgaacacaga agctttggga
    25321 acttctccta gaatgacttt cattaagcat aagtcataag actatctaga tatgattaag
    25381 taaactatta atcttattaa aggtctctag gaagaaaaaa tttcatgcga tgagattgtt
    25441 ttagaaaaag cgacgatata tataatctaa caataatttt taaaatatta agaaccccca
    25501 gcatatgctt tatatagctt gctgggggtt tctcatacaa tatggatcga gcatcttgca
    25561 tgtataaaca aataggctat tcaaagtaaa attactcaaa ctttgaatag cctttcctct
    25621 tcaaatagtc tttatttaaa ttggattatg ttattggatt ttcttaccta caaaaacgat
    25681 ttcttgatgt gatacttcaa ccgtgccatc tttttgctct tgaaacacgg gtttttcaat
    25741 acgttttacc ggcatatacc cttgcatttt cattcgtgac aaacaatcag caattgtttc
    25801 attctcattt actttgaatt tcatgttcta ctacactttc cagttcttct ggttcataat
    25861 ctttaagctt tctacgacga atgcctttag tccagaatcc accatgtatg tttcgaggtt
    25921 catacgcaat gataaatgct ttcggatcta aattttttat cgtatccatc aatttacgtt
    25981 catattttct tggtgttaaa atttgcatca ccatacgact accatctcta ccaaacgcag
    26041 catagtgcgt aacgccatat cctaaatttc gaagttcatt cggtaaatct aactcatatt
    26101 ctgctgaagt tacatttaca actgtataac ctaatgccag tttttcttct attttcatac
    26161 caacaatgat acctattgaa aaaccaaatg cgtaggcaat aatattttga atatggtcta
    26221 aattagacat aaccaaacct aaaccaacga tataaactaa tacttctaaa aaactaactg
    26281 atgcagcaat ataacgataa cctttcaacg ttaaaattgt tcgcatcgtt aaaaacgtta
    26341 cataacaaat gttaatgata aatatagtta gtaccattaa ccatggattt tctgttacga
    26401 atgacataag gcgccccact ctctttcaat tacactttaa ataccaaact gcttcaacct
    26461 aaccttaatg aatgcatatt aatataatat agtacgaaaa ctctcataaa taaatagcga
    26521 atttgtcata aataaaaatt tattctcaaa tgaacatact tactttagtt catacacttt
    26581 caatttatac actgaagtag tctagctgaa tgtggtttaa tgttattaat tctaactcat
    26641 atttaattta agtcacacgt tagaagaaaa aattaaatta ggattttggc cacgtgtatc
    26701 ttgtatatgc aagttcacgt ttgtgtgcat ttcgtatata atgattttca attacttttt
    26761 gttcttctgg cgtaactggc ttaccttcta aataattatc aatcgcctca taagttacac
    26821 ctaatgcatc ttcatctgga agctgtggtt tatcatcttc taaatcagca gttggcgttt
    26881 tttcatataa ttcctttggc gcaccaagat acgctaataa ttgacgacct tgtcgtttat
    26941 tcaaaccaaa tataggtgcg atatctgcag caccatcacc gtacttcgta taaaacccag
    27001 ttatattttc agctgaatga tctgttccta ctacaatacc ttgtcggttt gaagcaattg
    27061 aaaattgtac tttcatacgt tcacgcgctt tttcatttcc tttttggaaa tctgtaagaa
    27121 caataccggc ttcttttaat gattgcacac tttgatcaac tgcaggctta atattgactg
    27181 ttactatttc atctggttca atgaatcgca aagcttgctc aacttcatca gcatcttttt
    27241 gaactccata aggtaattta actgcaataa acgtacaatc aatgccttct tcacgtaatt
    27301 cgttaacaga catttgtact agttttccaa ctaatgtaga atcctgtcct cctgaaatac
    27361 ctaacactaa agattttata aatgaatgtg attgtacata attttttata aattgcttta
    27421 attccataat ttcttcagca ctatcgatac gctttttcac tttcatttct tgtacaataa
    27481 cgtcttgtaa tttactcatt atcttcttcc atctccttaa cgtgttccgc aacttcaaaa
    27541 atacgtttat gtttattatc ccaacatgcc ttgcttaaat cgactggata ttcttgtgga
    27601 ttcaggaaac gcttattttc atcccaaata gattgtaatc ctagtgctaa atattcacgt
    27661 gattcatctt ctgttggcat ttgatatact aatttaccat tttcataaat attatgatgc
    27721 aaatcaatgg cttcgaaaga ttttataaat ttcattttat aagtatgcac tggatggaat
    27781 aattttaaag gttgttcatc gtatggattt tcattttcca aagtaatata atcgccttct
    27841 gccttacctg ttttcttgtt tataatgcga tatacatttt tcttacctgg cgtcgtaacc
    27901 ttttcagcgt tatttgataa tttaatacga tcactatatg aaccatcttc attttcaata
    27961 gctacaagtt tatatactgc acctaatgct ggttgatcgt atcctgtaat cagctttgta
    28021 ccaacgcccc aagaatctac ttttgcacct tgtgctttca aactcgtaat cgtttcttca
    28081 tccaaatcat tagacgcgat aattttagtt tcagtaaatc ctgcttcatc aagcatacgt
    28141 cttgcctctt tagataaata agcgatatct ccagaatcta atcgaatacc tacaaagtta
    28201 attttgtcac ctaattcttt tgcaactttt attgcatttg gcacgccaga ttttaaagta
    28261 tggaatgtat ctactaggaa cacacaattt ttatgtcttt cagcatattt tttgaaggca
    28321 acatattcgt ctccataagt ttggacaaat gcatgtgcat gtgtaccaga cacaggtata
    28381 ccaaataatt tccccgccct aacattactt gtagaatcaa agcccccgat gtaagcagct
    28441 ctagcgcccc acaatgctgc atcaatttct tgcgcacgac gtgtaccaaa ctccattaat
    28501 ttatcatttg atgcaatttg acgaattctg ctagcctttg ttgtaattaa tgtatggaaa
    28561 tttacaatgt ttaataaaat tgtttctatt aattgcgctt gaatcaatgg tgcttctacg
    28621 cgtaacaatg gttcgttacc aaagcataat tcgccttctt gcatcgaacg gatgctgcct
    28681 gtgaatttta aatcttttaa atatgataag aaatcatcct tgtagccaat agactttaaa
    28741 tattccaaat cagattctga aaatccaaaa tgttctataa aatcaatgac gcgttttaaa
    28801 ccattaaaaa cagcatagcc actattaaat ggcatttttc taaaatacaa atcaaataca
    28861 gccatttttt catgaatatt atcattccaa taactttcag ccatatttat ttgatataag
    28921 tcattatgta acattaaact gtcgtcttct aattggtaca cttgtatctc tccaatcgac
    28981 ctaaatattt tcttacattt tatcataatt cattttttta tatacataag agccccttaa
    29041 tttccatact tttaattaaa atcaaccaac aatttaatga catatacata atttttaaga
    29101 gtattttaat aatgtagact ataatataaa gcgaggtgtt gttaatgtta tttaaagagg
    29161 ctcaagcttt catagaaaac atgtataaag agtgtcatta tgaaacgcaa attatcaata
    29221 aacgtttaca tgacattgaa ctagaaataa aagaaactgg gacatataca catacagaag
    29281 aagaacttat ttatggtgct aaaatggctt ggcgtaattc aaatcgttgc attggtcgtt
    29341 tattttggga ttcgttaaat gtcattgatg caagagatgt tactgacgaa gcatcgttct
    29401 tatcatcaat tacttatcat attacacagg ctacaaatga aggtaaatta aagccgtata
    29461 ttactatata tgctccaaag gatggaccta aaattttcaa caatcaatta attcgctatg
    29521 ctggctatga caattgtggt gatcctgctg aaaaagaagt tacacgctta gcaaatcact
    29581 taggttggaa aggaaaaggt actaattttg acgtgttacc actgatttac caattaccta
    29641 atgagtcagt taaattttac gaatatccta cttcattaat taaagaagta cctattgaac
    29701 ataatcatta tccaaaatta agaaaattga acttaaaatg gtatgcagtc cctatcattt
    29761 ccaatatgga cttaaaaatc ggtggcattg tatatccaac tgcacccttt aacggttggt
    29821 atatggtaac tgaaattggc gtacgtaact ttattgatga ttaccgttac aatttactag
    29881 aaaaagttgc agatgcgttt gaatttgata cacttaaaaa taattcattt aataaagatc
    29941 gagcacttgt tgaattgaac tatgctgtgt atcattcctt taaaaaagaa ggcgtatcaa
    30001 ttgtcgatca tttgaccgct gcaaagcaat tcgaactatt cgaacgtaac gaagcacaac
    30061 aaggtcgtca agttaccgga aaatggtctt ggctagcacc gccattatct ccaacattga
    30121 cgtcaaatta tcatcacgga tatgacaata cagtaaaaga tccaaacttt ttctataaaa
    30181 agaaagaatc aaatgctaac cagtgccctt tccatcatta agttagtaga ggtgtagcat
    30241 atgcaattat attatttagg ccctaaaggt acattttcat atttggcttg tcgtcagtat
    30301 ttttctgaaa atgaagcaac ttttcaacca aagtcaaatt tttttgaagt catcaaggca
    30361 gttgcagatg atgacacatc gattggtgtt gtccctattg aaaattctat agaaggaaca
    30421 atcaacattg tggcagatgc acttgcacag caagatgtct ttgcacacgg tgaaatacgt
    30481 ttagatatta actttgcatt atatggtaat ggaacagatt ccatttctga tattaaaaaa
    30541 gtgtattcta ttgcaccagc aatcagtcaa acaacaaatt acatacatca acatcaattt
    30601 gactatgatt atgtcgacag tacaattcaa agtttgacta aaattgaaaa tggcgttgca
    30661 gcaattgcac ctttaggtag tggcgaagct tatggtttta cacctattga tacacatatt
    30721 gaagactatc cacataacgt gacacgtttt ttagttataa aaaatcagca gcaatttgat
    30781 caaaatgcga catcattgat gtttttaatt acaccaatgc atgataaacc tgggttgtta
    30841 gcaagtgtgc ttaatacatt tgctttgttt aatattaatt tatcctggat tgagtctcgt
    30901 cctttaaaaa cacaattagg tatgtatcga tttttcgttc aggctgattc tgcaattaca
    30961 accgatataa agaaagtcat tgctatttta gaaaccttag atttcaaagt agaaatgatt
    31021 ggtgctttta attaaacttt attttctagg ttctacaatt taaataaaat aaagagtcaa
    31081 ccaaggatta aaataggaat ccatccttgg ttgactttct tttgtttgta ttgtttttac
    31141 acgatgctgc ggtatacgtt tcgttaccac tcgtccaagc aattgctaat gtaacctttt
    31201 atttttcgtt ctaatctgca attactattt caatggcagt ggttgattta tatcaatacc
    31261 taaaactata ggcattacat aatacacgac taaaataata attattgcac tgattaagtt
    31321 tacccagaat cctactgatg ccatttgttt gatagatatt ttaccagaac caaagataat
    31381 tgcattcggt ggtgtcccta ctggtaacat gtatgcacag ttagccgcca tagctgcagg
    31441 tgccataagt agtaatggat gcactccaac agcaacagac aacgttgcta aaatcggtaa
    31501 aatcatcgtt gcagttgcag tattagatgt cacttcagtt aaaaataaga caaagattgt
    31561 tatgacaatt acaataagaa tcggactaac accatttaat gatttcaact gttcgcctaa
    31621 ccattttgct aaaccacttt cagaaatacc tttcgctaat gctaaaccgc caccaaataa
    31681 aattaataca ccccaaggga gctcttttgc aacttcccag tcaatgatac ggcgatgttt
    31741 ttcagtattt ttagctggaa taataaataa taatattgat ataaaaatag caatcgtacc
    31801 atctgcaaca gatgacgtaa cttcccattt tttcagaaga aactctcttg taatccataa
    31861 taagctagca agtacaaaga tagtttgtac taccttttct tcatacttca ttttgccaag
    31921 ctcgtctaac ttttgtttaa ttaacgtctg accaccaggt aaatatttca aatcatgtct
    31981 aaacgcaaca tatcttaaat agagccaagt aatacctaac aaaacaatga ccgttggaat
    32041 ccctacaatc atccatttag caaaactaat ttcatatcca aaatgttgca tgtattgtcc
    32101 ttttaaaata attaatggcg gggttccgat taatgtaccc aagccaccaa tcgtacctgc
    32161 atagccaatt gctaaaacta gagatttttc aaacttttga atacttgttt gattcgtatt
    32221 ggcttcttgt aaatcatgtg cttccttaat aattgctaaa ccaatcggaa tcataatcat
    32281 tacagctgca gtgttcgata caaacataga taagaatcct gttgccacca tgaatccaag
    32341 taatattttt gaagtgcttg caccaatcaa attaataatt gtcaacgcta ctctcgtatg
    32401 taaattccat ctttccattg caattgccaa aataaatcca cctaaaaaca aaaagataat
    32461 atcattgcca tattcggatg atacttgttc tggtgtaagt atatgaccta atggtaataa
    32521 cacaattggt aataagctcg ttgctgcaat aggaattgct tcagtaatcc accaagtcgc
    32581 aatccataaa gtaatcgcta aaacatagac gcctttccaa ggtaagtctt gtggatgaaa
    32641 gaataataat gttaatagga aaagtaatgg acctaatatt agtccaataa gttgtcccgc
    32701 actaaacggt ttctttttct ttgattttga aaagaatgtt aaataccttt tcgatatcat
    32761 tgattgatgt tgattgaaat aagccataaa tttccccctc atgatgatta tgacaagcta
    32821 cttcaactca ctatattaga gtatagaaag ttgtctatct tgtattatca ttaccgtcaa
    32881 aatacccctt ttatcagata attgggttta ttatagcata tttataccgt tttatgaatg
    32941 cgctttcaaa aattatctac tattcttaat cctaatgtca tgcgaaactt tttacttttt
    33001 tatttcatct acattgattg tagtaacttg aatacccgct tcaacaggac ttaaaaacac
    33061 tgtatcaata tcttctaaat gaattgattg agtcgaatta ttgaatgtat tcccaacgat
    33121 taatacatct ttatgtttaa cattattata attacgtaca tgtatcgcat ctttagacat
    33181 tgatccttta aattcatttc caattatatt catatttatg cctgcttggg aacctaagtc
    33241 ttttcctgtc atcacatctg ctgcattttt accatctcta actcctaaat atctaatgcc
    33301 accctcacag ttaataaact tattattaat aatgaaagca tctttatact tcaagggagt
    33361 taatgcataa ccttgtatat cttcaaatat attatttcta atatgaatat tctcatagta
    33421 tcgattatgt ctacttgcat gtgagccaat agcacgattc caacttccca tttcgggcaa
    33481 ttctgaaggt ccaaaataac aatcttcgat aatgacattt ttcgttatcg taccatctgt
    33541 cgttccgaat tttggaaaag caccaggtac ttgaatgtct aattgtattg cttcagaata
    33601 aaagcgttcg ccactatagt ctatgaatcc ttcaaatgaa cagcttttaa tatagagtcc
    33661 gttaatccca caagcatcaa ttgcatgacc actcactaca ttcttaatgg tcacacctat
    33721 taattgaata tcttcagcat gcccaatgca catagctgta ttgttataag gatacgatac
    33781 accattcata tcaaacttgc cgcctttaat atgaatatga ctgtgtccat tataaccacg
    33841 ataaaaacca aagcgacgac cattttttaa taaaggacca gaatgacatc ggcgtaaaat
    33901 agtttcatta tctaacaaaa gtgttgtatt gccatatatc gttaatggtt tgcaaatatc
    33961 atacgtccct ttcggtatat aaactgtcgt tggtttacat cttccacgat ttaacgcacg
    34021 ttgtattgct cgagtatctt tagttttgtt tatgcctgtt aacccataat ctttcatatt
    34081 gatatacata aatcgtcatc ctttgtctat tttctaatta ctcatatatt aaccaattat
    34141 tcaaattata atcgtgttag gttgatttaa cagatagtca taaatctcgt gatgccatct
    34201 attagcattt ttatattcaa aacatcaaca cttgatatta gttctaagtt tgtcacattt
    34261 agtattctat tacagctatt ttaaaactat cgcaaaaact tcttatatta tttgttgatt
    34321 gcgtgatttg ctcttccaaa a
//
LOCUS       DANRDI010000011        33796 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.011, whole genome shotgun sequence.
ACCESSION   DANRDI010000011 DANRDI010000000
VERSION     DANRDI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33796
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.011"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            72..836
                     /locus_tag="PZ377_000368"
     CDS             72..836
                     /locus_tag="PZ377_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6357260.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(828..1079)
                     /locus_tag="PZ377_000369"
     CDS             complement(828..1079)
                     /locus_tag="PZ377_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357261.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            1468..2733
                     /gene="femX"
                     /locus_tag="PZ377_000370"
                     /gene_synonym="fmhB"
     CDS             1468..2733
                     /gene="femX"
                     /locus_tag="PZ377_000370"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDM6357262.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            2850..6017
                     /locus_tag="PZ377_000371"
     CDS             2850..6017
                     /locus_tag="PZ377_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDM6357263.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(6204..6521)
                     /gene="mspA"
                     /locus_tag="PZ377_000372"
     CDS             complement(6204..6521)
                     /gene="mspA"
                     /locus_tag="PZ377_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDM6357264.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(6766..6930)
                     /locus_tag="PZ377_000373"
     CDS             complement(6766..6930)
                     /locus_tag="PZ377_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357265.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            7063..7971
                     /locus_tag="PZ377_000374"
     CDS             7063..7971
                     /locus_tag="PZ377_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDM6357266.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            8142..8273
                     /locus_tag="PZ377_000375"
     CDS             8142..8273
                     /locus_tag="PZ377_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357267.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(8390..9253)
                     /locus_tag="PZ377_000376"
     CDS             complement(8390..9253)
                     /locus_tag="PZ377_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HDM6357268.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(9434..10321)
                     /locus_tag="PZ377_000377"
     CDS             complement(9434..10321)
                     /locus_tag="PZ377_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357269.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            10387..10482
                     /locus_tag="PZ377_000378"
     CDS             10387..10482
                     /locus_tag="PZ377_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357270.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            10508..12643
                     /locus_tag="PZ377_000379"
     CDS             10508..12643
                     /locus_tag="PZ377_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDM6357271.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            12756..14090
                     /locus_tag="PZ377_000380"
     CDS             12756..14090
                     /locus_tag="PZ377_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDM6357272.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(14282..14671)
                     /locus_tag="PZ377_000381"
     CDS             complement(14282..14671)
                     /locus_tag="PZ377_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357273.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            15028..15336
                     /gene="rpsJ"
                     /locus_tag="PZ377_000382"
     CDS             15028..15336
                     /gene="rpsJ"
                     /locus_tag="PZ377_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDM6357274.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            15364..16026
                     /gene="rplC"
                     /locus_tag="PZ377_000383"
     CDS             15364..16026
                     /gene="rplC"
                     /locus_tag="PZ377_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDM6357275.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            16053..16676
                     /gene="rplD"
                     /locus_tag="PZ377_000384"
     CDS             16053..16676
                     /gene="rplD"
                     /locus_tag="PZ377_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDM6357276.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            16676..16951
                     /gene="rplW"
                     /locus_tag="PZ377_000385"
     CDS             16676..16951
                     /gene="rplW"
                     /locus_tag="PZ377_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDM6357277.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            16984..17817
                     /gene="rplB"
                     /locus_tag="PZ377_000386"
     CDS             16984..17817
                     /gene="rplB"
                     /locus_tag="PZ377_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDM6357278.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            17884..18162
                     /gene="rpsS"
                     /locus_tag="PZ377_000387"
     CDS             17884..18162
                     /gene="rpsS"
                     /locus_tag="PZ377_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDM6357279.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            18191..18544
                     /gene="rplV"
                     /locus_tag="PZ377_000388"
     CDS             18191..18544
                     /gene="rplV"
                     /locus_tag="PZ377_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDM6357280.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            18568..19221
                     /gene="rpsC"
                     /locus_tag="PZ377_000389"
     CDS             18568..19221
                     /gene="rpsC"
                     /locus_tag="PZ377_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDM6357281.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            19224..19658
                     /gene="rplP"
                     /locus_tag="PZ377_000390"
     CDS             19224..19658
                     /gene="rplP"
                     /locus_tag="PZ377_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDM6357282.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            19648..19857
                     /gene="rpmC"
                     /locus_tag="PZ377_000391"
     CDS             19648..19857
                     /gene="rpmC"
                     /locus_tag="PZ377_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDM6357283.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            19881..20144
                     /gene="rpsQ"
                     /locus_tag="PZ377_000392"
     CDS             19881..20144
                     /gene="rpsQ"
                     /locus_tag="PZ377_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDM6357284.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            20176..20544
                     /gene="rplN"
                     /locus_tag="PZ377_000393"
     CDS             20176..20544
                     /gene="rplN"
                     /locus_tag="PZ377_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDM6357285.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            20580..20897
                     /gene="rplX"
                     /locus_tag="PZ377_000394"
     CDS             20580..20897
                     /gene="rplX"
                     /locus_tag="PZ377_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDM6357286.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            20924..21463
                     /gene="rplE"
                     /locus_tag="PZ377_000395"
     CDS             20924..21463
                     /gene="rplE"
                     /locus_tag="PZ377_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDM6357287.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            21486..21671
                     /locus_tag="PZ377_000396"
     CDS             21486..21671
                     /locus_tag="PZ377_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDM6357288.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            21703..22101
                     /gene="rpsH"
                     /locus_tag="PZ377_000397"
     CDS             21703..22101
                     /gene="rpsH"
                     /locus_tag="PZ377_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDM6357289.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            22126..22662
                     /gene="rplF"
                     /locus_tag="PZ377_000398"
     CDS             22126..22662
                     /gene="rplF"
                     /locus_tag="PZ377_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDM6357290.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            22693..23052
                     /gene="rplR"
                     /locus_tag="PZ377_000399"
     CDS             22693..23052
                     /gene="rplR"
                     /locus_tag="PZ377_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDM6357291.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            23073..23573
                     /gene="rpsE"
                     /locus_tag="PZ377_000400"
     CDS             23073..23573
                     /gene="rpsE"
                     /locus_tag="PZ377_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDM6357292.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            23590..23769
                     /gene="rpmD"
                     /locus_tag="PZ377_000401"
     CDS             23590..23769
                     /gene="rpmD"
                     /locus_tag="PZ377_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDM6357293.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            23786..24226
                     /gene="rplO"
                     /locus_tag="PZ377_000402"
     CDS             23786..24226
                     /gene="rplO"
                     /locus_tag="PZ377_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDM6357294.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            24226..25518
                     /gene="secY"
                     /locus_tag="PZ377_000403"
     CDS             24226..25518
                     /gene="secY"
                     /locus_tag="PZ377_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDM6357295.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            25535..26182
                     /locus_tag="PZ377_000404"
     CDS             25535..26182
                     /locus_tag="PZ377_000404"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDM6357296.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            26375..26593
                     /gene="infA"
                     /locus_tag="PZ377_000405"
     CDS             26375..26593
                     /gene="infA"
                     /locus_tag="PZ377_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDM6357297.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            26625..26738
                     /gene="rpmJ"
                     /locus_tag="PZ377_000406"
     CDS             26625..26738
                     /gene="rpmJ"
                     /locus_tag="PZ377_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDM6357298.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            26761..27126
                     /gene="rpsM"
                     /locus_tag="PZ377_000407"
     CDS             26761..27126
                     /gene="rpsM"
                     /locus_tag="PZ377_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDM6357299.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            27150..27539
                     /gene="rpsK"
                     /locus_tag="PZ377_000408"
     CDS             27150..27539
                     /gene="rpsK"
                     /locus_tag="PZ377_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDM6357300.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            27614..28558
                     /locus_tag="PZ377_000409"
     CDS             27614..28558
                     /locus_tag="PZ377_000409"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDM6357301.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            28575..28943
                     /gene="rplQ"
                     /locus_tag="PZ377_000410"
     CDS             28575..28943
                     /gene="rplQ"
                     /locus_tag="PZ377_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDM6357302.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            29481..30290
                     /locus_tag="PZ377_000411"
     CDS             29481..30290
                     /locus_tag="PZ377_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDM6357303.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            30287..31147
                     /locus_tag="PZ377_000412"
     CDS             30287..31147
                     /locus_tag="PZ377_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDM6357304.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            31137..31943
                     /locus_tag="PZ377_000413"
     CDS             31137..31943
                     /locus_tag="PZ377_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDM6357305.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            31948..32751
                     /gene="truA"
                     /locus_tag="PZ377_000414"
     CDS             31948..32751
                     /gene="truA"
                     /locus_tag="PZ377_000414"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDM6357306.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            32991..33428
                     /gene="rplM"
                     /locus_tag="PZ377_000415"
     CDS             32991..33428
                     /gene="rplM"
                     /locus_tag="PZ377_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDM6357307.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            33442..>33796
                     /gene="rpsI"
                     /locus_tag="PZ377_000416"
     CDS             33442..>33796
                     /gene="rpsI"
                     /locus_tag="PZ377_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDM6357308.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKP"
BASE COUNT        12243 a         5167 c         6435 g         9951 t
ORIGIN      
        1 taattattaa gagcaaaaat aattatactt tgttaaaata agcgtaatta catgtaaata
       61 gggggatact aatgatattg aaatttgatc acatcattca ttatatagat cagttagatc
      121 ggtttagttt tccaggagat gttataaaat tacattcagg tgggtatcat cataaatatg
      181 gaacattcaa taaattaggt tatatcaatg aaaattatat tgagctacta gatgtagaaa
      241 ataatgaaaa gttgaaaaag atggcaaaaa cgatagaagg cggagtcgct tttgctactc
      301 aaattgttca agagaagtat gagcaaggct ttaaaaatat ttgtttgcgt acaaatgata
      361 tagaggcagt taaaaataaa ctacaaagtg agcaggttga agtagtaggg ccgattcaaa
      421 tggaaagaga tacacataaa gatggtaagg taaagtggca attgctttat ataatgaatc
      481 aggatgatga tgaaattaag ccaccatttt ttattcaatg ggaagaaagt gattccatgc
      541 gtactaaaaa attgcaaaaa tattttcaaa aacaattttc aattgaaact gttattgtga
      601 aaagtaaaaa ccgatcacaa acagtatcga attggttgaa atggtttgat atggacattg
      661 tagaagagaa tgaccattac acagatttga ttttaaaaaa tgatgatatt tattttagaa
      721 ttgaagatgg taaagtttca aaatatcatt cggttatcat aaaagacgca caagcaactt
      781 caccatattc aatttttatc agaggtgcta tttatcgctt tgaaccatta gtataaatat
      841 acgtaagtgc tatgagcgag aatgcccata tgaataatga caagcacaat ggaaagaatc
      901 gttaatatat tatttaatcg tgatgactta attaaaatga aaaagattga taatataaat
      961 gtgaaaaaga taagtataac ccgtaaacta aagtaattca cggtgagagg ttgactcaat
     1021 gtcataatga ttgcaacgat gttcataatt ataaatagac ttaaaataat tgttctcata
     1081 tcaaacacct cattgttaga ttattgacat tataacaggg gtaattgtat atgaacatta
     1141 atgtggttgc ttgaggaaaa atttattcat tgaagtcaag ttggttcatt ttagaaatga
     1201 atatcgtgtt agatgatgaa agtatattga agtataggta actagttgaa aagtattaat
     1261 tgtacgataa cattaaattt aacacgaaac atagatataa aatgattcac aattaaaatg
     1321 ggtaaatttg aacttgctaa actattaatt ggagcatgga catttcaaaa ataagagttc
     1381 aaatcttaca caagctctga atcgacacta taagatacaa actgtataat taaaggtatt
     1441 gttaaataga aggagatatc ataaatcatg gaaaagatgc atatcactaa tcaggaacat
     1501 gacgcatttg ttaaatccca cccaaatgga gatttattac aattaacgaa atgggcagaa
     1561 acaaagaaat taactggatg gtacgcgcga agaatcgctg taggtcgtga cggtgaagtt
     1621 cagggtgttg cgcagttact ttttaaaaaa gtacctaaat taccttatac gctatgttat
     1681 atttcgcgtg gttttgttgt tgattatagt aataaagaag cgttaaatgc attgttagac
     1741 agtgcaaaag aaattgctaa agctgagaaa gcgtatgcaa ttaaaatcga tcctgatgtt
     1801 gaagttgata aaggtacaga tgctttgcaa aatttgaaag cgcttggttt taaacataaa
     1861 ggatttaaag aaggtttatc aaaagactac atccaaccac gtatgactat gattacacca
     1921 attgataaaa atgatgatga gttattaaat agttttgaac gccgaaatcg ttcaaaagtg
     1981 cgcttggctt taaagcgagg tacgacagta gaacgatctg atagagaagg tttaaaaaca
     2041 tttgctgagt taatgaaaat cactggggaa cgcgatggct tcttaacgcg tgatattagt
     2101 tactttgaaa atatttatga tgcgttgcat gaagatggag atgctgaact atttttagta
     2161 aagttggatc caaaagaaaa tatagcgaaa gtaaatcaag aattgaatga acttcatgcc
     2221 gaaattgcta aatggcagca gaagatgaaa acatctgaaa agcaagctaa aaaagcgcaa
     2281 aatatgatta atgatgcgca aaataaaatt gctaaaaatg aagatttaaa acgagaccta
     2341 gaagctttag aaaaggaaca tcctgaaggt atttatcttt ctggtgcact attaatgttt
     2401 gctggctcaa aatcatatta cttatatggt gcgtcttcta atgaatttag agatttttta
     2461 ccaaatcatc atatgcagta tacgatgatg aagtatgcac gtgaacatgg tgcaacaact
     2521 tacgatttcg gtggtacaga taatgatcca gataaagact cagaacatta tggattatgg
     2581 gcatttaaaa aagtgtgggg aacatactta agtgaaaaga ttggtgaatt tgattatgta
     2641 ttgaatcagc cattgtacca attaattgag caagttaaac cgcgtttaac aaaagctaaa
     2701 attaaaatat ctcgtaaatt aaaacgaaaa tagattaacg actgaaatct gaacgctcat
     2761 aagactgtca tttgcgttca gattttttta cacaatatag aatggttgag taaaatattt
     2821 ttgaatatag tgaaagaggg ggaagtactg tgataaaaaa gctattacaa ttttctttag
     2881 ggaataagtt tgctatcttt ttaatggttg ttttagttgt cttgggcggt gtatatgcga
     2941 gtgctaaatt gaaattagaa ttactaccaa atgtacaaaa tccagttatt tcagttacaa
     3001 caacaatgcc gggtgcaacg ccacaaagta cccaagatga aataagtagt aaaattgaca
     3061 atcaagtaag atcattggca tatgtgaaaa atgttaaaac gcaatccata caaaatgctt
     3121 caattgtaac agttgaatat gaaaataata cagatatgga taaagcagaa gaacagctta
     3181 aaaaagaaat cgataaaatt aaatttaaag atgaagttgg tcaaccagaa ttaagacgta
     3241 attcgatgga tgcttttccg gttttagcat attcattttc aaataaagag aatgacttga
     3301 aaaaagtaac gaaagtactg aatgaacaat taataccaaa attgcaaacg gtagatggtg
     3361 tgcaaaatgc gcaattaaat gggcagacga accgtgaaat cacccttaaa tttaagcaaa
     3421 atgaacttga aaaatatggg ttgactgctg atgatgtaga aaactatcta aaaacggcaa
     3481 caagaacaac gccacttgga ttgttccaat ttggtgataa agataaatca attgttgttg
     3541 atggtcaata tcaatctgtt gatgctttta aaaacataaa tattccatta acgttggcag
     3601 gaggacaagg gcaatctcaa tcccaaagtg acaataaaca aaattcagcc atgtcagacg
     3661 ttaatcaggc atcaccacag caaaattcaa aagcgtcagc accaaataat ataagtggta
     3721 tgccgacagc gaaactagga gatttagctg atattacagt tggtgatgtg cgtacttcta
     3781 tttctaaaac gaatggaaag gatgcggtta atctacaaat aactaaagct caagatgcca
     3841 atacagttca agtagccaaa gatgtacaac gtaaaattga tacattcgtt gatgaaaata
     3901 aagattttaa tgtcacaaaa acaatggata ctgcaaagcc tgttgagaaa tcactttata
     3961 cgatggttga aaaagcatca ttaggtacaa tcgtggcaat tatagttatt ttgctgtttt
     4021 taagaaacat tcgtacgacg gcaatttcta ttatatcgat tccgttatca cttcttatgg
     4081 cgcttattgc tctgaaattg agtgatgttt cattgaatat actaacgtta ggtgcattaa
     4141 cagtagcgat tggacgtgtg atagacgatt cgattgtagt tgttgaaaat atttatcgac
     4201 gcttaacaga ttcagaagaa caactaaaag gtgaaaattt aattatcagt gcgacaactg
     4261 aagtatttaa accaataatg tcatcgacac tagttactat tatcgtcttc ttaccacttg
     4321 tgtttgtatc aggttcagta ggcgaaatgt ttagaccttt tgcattggct attgcattta
     4381 gtttattagc atcgttatta gtgtcaatta cactcgttcc agcgttggca gctacactat
     4441 ttaaaaaagg cgttaaacgt cgtaataaac aacatcaaga aggattaggt gttgttagta
     4501 caacttataa aaaagtatta cattggtcat taaatcataa gtggattgta attatattaa
     4561 gtacattaat tttggttgca actattgtat ttggaggacc gagactaggc actagcttta
     4621 tttcagcagg tgacgataaa tttttagcta ttacttatac accgaagcct ggtgaaacgg
     4681 agcaagcagt gttgaatcat gcgaaagatg ttgaaaaata tttaaaacag aaaaagcatg
     4741 taaaaacaat tcaatactca gttggcggta gtagtccagt agatccaacg ggtagtacaa
     4801 atagtatggc aatcatggtt gaatatgata atgacacgcc taattttgat gtagaagcgg
     4861 ataaggttat taaacatgca gatggcttta aacatcctgg agagtggaaa aatcaagatt
     4921 taggaacagg tgcaggtaat aaatctgtag aggttactgt aaaaggtcca tcaatggatg
     4981 ccataaaatc aactgtaaaa gatattgaac agaaaatgaa acaggttaaa ggactagcca
     5041 atgtcaaatc tgatttatcg caaacatatg atcagtatga aattaaagtc gatcaaaata
     5101 aagcggcaga aaatggtatt tctgcaagtc aacttgcaat gcacttgaat gaaaacttac
     5161 cagaaaaaac agttacgact gttaaagaaa atggtaaaac tgttgatgtt aaagtcaaac
     5221 aaaataagca aacagactgg tcagaagata agttgaataa tataactttg aaaaagccga
     5281 ctggtggtac gattaaattg ggagatatcg ctacgttagt taaaacaacg acaccaagta
     5341 aattgacgca agaacaagga gattatgcaa cgacggtatc tgctaaagta acaaataaag
     5401 atgtgggtgg cacaacacga caagtgatgt ctaaaataaa taatttggac aaaccgaata
     5461 atgtaaaggt taatatcggt ggtgcatcag atgatattaa caatgcaatg acacaattag
     5521 cctttgcaat gttagctgca atcattatcg tatatttaat cctagttatt acatttaaag
     5581 gtggcctagc accatttaca attttattct ctttaccatt tacagttatc ggtgtaatta
     5641 ttgcactatt aatcacagga gaaacaatat cagtaccaag tttaattggt atgctaatgt
     5701 taattggaat cgtagtaaca aatgccattg tgttaataga ccgtgttatt aataatgagc
     5761 aacagggcat ggagatgaaa gaagcattaa tcgaagcagg cggtactaga attagaccga
     5821 tattaatgac ggcgattgca acaattggtg cattagttcc tttgttattt ggtcaagata
     5881 gctcgattct tatttcgaaa ggattagctg ctacagttat tggtggttta atttcatcaa
     5941 ctttattaac acttgtagtt gtaccagtaa tatatgaaat tctctttact ttaaaaaaac
     6001 gattcactaa acgatagata atataaaagc cataaaagcg gtcatgatat tgactacttt
     6061 tatggctttt taaaatttga aattttagac cttatttcac gtgcgattta atcgatgtgt
     6121 taataaaaca atactggcat tcaacatgta atccgataat aaaataaggt tgcaattatg
     6181 ttattgcata ttttgtttat tacttagaat aatacgatgg ttaagatgaa atatatgaca
     6241 aagataaata atgacattaa attcaagatg tttacgcctt ttagatcttt tttaagcatt
     6301 ttaaatcctg tcacttcgac attaccgact aagagtaata agacgataaa tacccaccaa
     6361 ttctcttttg gaaaactcat cgtcgttaat gctaagacga ataataacaa cacacccata
     6421 ataattctca aaatgcgagt aaatacaact tccattttaa aaatactaat aaaactaaca
     6481 attagataaa gtattgctag taaaatcaga taaaattgca tatgataact tcctctccgt
     6541 ttcaaagggt tcatgtagta cagcagcgta gaaaacataa atagcagtgt tgggatagat
     6601 atgcgtgtat gttgttaatg aaacactaaa tcataacacg acaacatctt gtgattctag
     6661 atgtaattga gcgaaaaatg aagcaattca tcaatataac gtattcagag caatgctagt
     6721 caaatttatg taacaaaaag catttattgc ttgaatgata gattattaat cttgcatttg
     6781 agcacgaacg tttctaattt cattttcaat atctttaagt tgacgaacaa gtgcggaagt
     6841 atctaaattc aaagattccc gtttttctat gtcaccttga agacgaacat aatcatattt
     6901 aagttcggct agttggtttt ctaaagacat actataacac tcctttgaaa tataattaag
     6961 tgcatttaat gtagattata gcatgaaaca gtagacttct aaaatatctg tggagtatgt
     7021 taaagttata aattatgaag attattgaaa gaagggataa cagtgacaga acaatttact
     7081 atgattattt tattaattgc gcttggttac tttttgaagc gcattaattt tataaaagca
     7141 acagatagtc aagtcattgc tacattagtt cttaatgtca cactcccatc tttagttatt
     7201 gtgaatttaa acagtgcaga attaaaattg tcattctcca ttttaccaat tttaatgatt
     7261 atatatggta ttgtggcgaa aatgattgct gtctggtttt ttagaaaata tgataatcac
     7321 atgcgtggat cagtaggtat gatgactggt gctatgaata ttggattgtt tgcatatcca
     7381 ctaattgaag tgatatggcc taaaacggga cttatttatt ttgttatggc tgatataggt
     7441 ggtgcctttg tgatgtttgg aattacttac tttgtaggta gctactttag tgaaggtagt
     7501 gatcaattcg attttaaata tttaggtaag aaattattac aatcagtgcc actcgttaca
     7561 tacattgtaa tgtttatatt gaatataagt cacatccatt taccacatgt ggcaatcgat
     7621 ttctttagca tactttccca tgcaaatatg ccgttatcaa tgattttgct aggtgtcatg
     7681 ttgaatttta ctctggaaag aaagtatata cctgctacta ttaaatattt atgtttgcat
     7741 tatggattag cattagttgc gggattgtta gtatatgtgt tgttaccagt atcagatgat
     7801 atgattaaga cgacattgtt ggtcacttgg atgttcccgg taggtgttgc aattatttct
     7861 tatggtattc agtttaaata tcgaacactg acatttatag gtatgacgac caatttgaca
     7921 atcattatta gtatcattat tttgtacgtg tatcaggcag tgtttgtcta gtttacacat
     7981 ttgaaaataa aatccatttt agtattcgta gaaaatgaga gcccccttta ccacaatatg
     8041 taaatgtata ttgtgaaaag ggggctttta tttatgacat agttgttcaa ctcatacatt
     8101 caagttgata atgcagtgcg ttatgcagct atccacttca tatgtatggc gcatacttga
     8161 tatatgaata tcatcaagat tttaaaagta tcgctaattt taaagtctta aaacgcatga
     8221 ttaatgtcat gatgtgttat tgcgttttgt ttttagagcc acgtatttcc taggacatat
     8281 agaggaacat tcaacgtaaa attcccgaaa attaatttta atcattctat aagtgaatgg
     8341 ttattttcag aagtgattac atttttacgt tacatgagta tttaactttc tatttcaaat
     8401 tacctagaat tatagcagct atcacgataa taataatacc tgcccaaata cccgtcatct
     8461 gacgacgatc ttttctttct cctaaaatga aaataccgcc taaggttgaa acgataacaa
     8521 gtaactgtga taatgagaaa cttgtagcta caccaacttt tggttgagaa tagaacatga
     8581 acaagttacc aattccccaa atcacacctg gcaatagatt aagtgctgtt gatttaagtg
     8641 atgttttatg attcatggat aggataaagc caccaattgc cataccgaca gattggaaga
     8701 acaatgcatc agttccacca acaccaaaga tgtcaccaag tacaacgaaa cctacatatc
     8761 caactgtaga tacaattaaa atacccattg cttttttaaa ttcaggatta tctgattgac
     8821 gttcattttt agctttaagt gaagtaagtg ctacaccagt aactaataat atcatggcga
     8881 ttaaaccaaa gataatttga gtcattgaag accattcacc taaaaagata acgctgaata
     8941 atgttgtacc aactaattgc atacctgtag aaactggcat agtatttgat acaccgatta
     9001 aactaataga tttaagctga ttcgcttgtc caaacgccca taatgcacca gaaataagac
     9061 cgacaataat tacagtagga ttattgaatt ttgcgaaacc agtaataagt aaacctaatc
     9121 caacaatgag tgcgcctagc gtcgtaccac gaatttggtt gtaaggtcca ccgccgacga
     9181 acacattaat aagaacgaca cttccccaga ataaagcagg taaaagtgcg attaagaaat
     9241 caagaaattg caagactact cactctccta tactatttat gcaagtgctc cccttaattg
     9301 acacttgcat gttattgtaa agcattctgc cctaatagta aaaaacaact acttataatt
     9361 ttcaatattt acaaaaagta gaaaaaccca caatacatgc taatgatgta ctgtagggta
     9421 tttgcttggt gttttattca gggattgttt gatcttttac gttacgtgat tcgaatttga
     9481 atttaatgta ctcatagttg atatggaaac ctagaccatt aaatcgttcg atgagtttat
     9541 tatttttatt acgaatttta aaataaactt ttctaatatc aaaattatca aaagcatatt
     9601 gcaatgcata cgcaagcaac tcgaaagcga tacctttcaa acggtaatct acatgagaac
     9661 tgaagtattt aatctcggcg attgactgtt gtgaatcaat ttcaaggtat aaataacctt
     9721 taagtaaacc ttcgctaaca aataaaaaca aatgatgatg gtcgtctaaa ctatcaatga
     9781 tatcttgtgg tgactgtgcc tgatatttaa atgtagttgt gtgtaatttg ctgaaagcac
     9841 gataaaaccc tttgtgataa ggaatgatat ttggttggtg catatcttct tctaatcttg
     9901 tacgggcttc tatgtaatag tcagtgaagt tataacttgc ttgaataact ttcattaatg
     9961 gtttgtattg ttgaatgcct tcttcaaaag aaaagttaaa gacggcatcg tcaggttggt
    10021 tcgaagtcat tgctttaaat agcgtttcaa acgtatcgac agataataca taattgtcag
    10081 ccacgaaagg gcctatgact ttgaatttat ttttatcgta tgcaaaagac aatatgattg
    10141 ctttgattga atcgttttct ttaatcgaaa atacaccagg tgattcaatc gcatcggtta
    10201 ttaatacatc tatttcattg tactgttgag gaagtttata taagtatgat gctgattcat
    10261 aattcgaatg ttcaatgaac gattttattt gtgtaatatc atataactga actatgctca
    10321 tgtcattacc tccgtacttt ttgttacttt tattatatag tatttcaact gaaatgaaag
    10381 ttaatagtga tattaacatg ttacaataca tttaacacat catttgcaat cattgaattt
    10441 aaatcaaaga ttagtggaat agatgaagca cgttcgaaat aaaagaacgt atgagaaagg
    10501 ataatttatg aaatctttaa tattagctga aaaaccatca gtcgcaagag atattgctga
    10561 tgctttacaa ataaatcaga agcgtaatgg ttactttgaa aataaccaat atattgtcac
    10621 gtgggcgtta ggtcatctag tgacaaatgc gacacctgaa caatacgata aaaatttaaa
    10681 ggaatggcga ttagaagacc ttccaattat acctaaatat atgaaaactg ttgttattgg
    10741 taaaacaagc aaacaattta aaacagtaaa agcgttaatt ttagataata aagtgaaaga
    10801 tattattatt gcaacagatg ctggacgaga aggtgaacta gttgcaagat tgattttgga
    10861 taaagttggt aacaaaaagc caatccgtcg attatggatt agctcagtta ctaaaaaagc
    10921 tattcaacaa ggttttaaaa atttaaaaga cggtcgtcaa tataacgatt tgtattatgc
    10981 agcgttagcg agaagtgagg cagattggat tgttgggatt aatgcaacgc gtgcactaac
    11041 aacaaagtat gatgcacagc tatccctggg acgtgttcag acaccaacga ttcaattagt
    11101 aaatacacga caacaagaga ttaatcagtt caaaccacaa caatacttta cattatcatt
    11161 aacggtaaaa gggtttgatt ttcagctaga atcaaatcag cgatatacca ataaagaaac
    11221 tttagaacag atggttaata atttgaaaaa tgtcgatggt aagattaaat ctgttgctac
    11281 taaacataag aagtcgtatc cgcaatcact gtacaattta acagatttac aacaagatat
    11341 gtatagacgt tataaaattg gacctaaaga aacattgaat acacttcaaa gcttatatga
    11401 gagacataaa gtcgtaacct atccaagaac agattcaaac tatttaacaa ctgatatggt
    11461 agatactatg aaagaacgta ttcaggcgac gatggcaaca acatataaag accaagcacg
    11521 cccattaatg tctaaaacat tttcatcaaa aatgtcgata tttaataatc aaaaagtatc
    11581 tgatcaccat gcaattattc ctacagaagt gagacctgtc atgtcagact taagtaatag
    11641 agaattaaag ttatacgata tgattgtcga gcgtttttta gaagctttaa tgcctccgca
    11701 cgagtatgac gcgataactg taactttaga ggttgcaggg cacacatttg ttttgaaaga
    11761 gaatgtaaca actgttttag gttttaaatc tattagacaa ggtgaatcta ttacagagat
    11821 gcaacagcct ttttcagaag gcgatgaagt gaagatttca aaaacaaata ttagagaaca
    11881 tgaaacaaca cctccagaat attttaatga aggttcgtta ttaaaagcga tggagaaccc
    11941 tcagaacttt attcaattga aggataaaaa atatgcgcaa actttaaaac aaacaggtgg
    12001 tatcggcaca gttgcaacaa gggccgacat tatcgataaa ttatttaata tgaatgccat
    12061 tgaatcaaga gacggtaaaa ttaaagtaac gtcaaaaggt aaacaaatat tagaattagc
    12121 accagaagaa ttaacgtcgc cacttttaac tgcacaatgg gaagaaaaat tacttttaat
    12181 tgaacgtggt aaatatcagg cgaaaacatt tattaatgaa atgaaagatt ttacgaaaga
    12241 tgttgtaaat gggattaaaa atagtgatcg taaatataaa cacgataatt taacaaccac
    12301 agaatgccca acgtgtggta aattcatgat taaagttaaa actaaaaatg gtcagatgct
    12361 tgtgtgccaa gatccatctt gtaagacgaa aaagaatgta cagcgcaaaa caaatgcaag
    12421 atgtccaaac tgtaaaaaga aattaacgtt gtttggtaaa gggaaagaag cggtatatcg
    12481 ttgtgtttgt ggacattctg aaacgcaagc acatatggat cagcgtatga agtctaaatc
    12541 ctctggtaaa gtatctcgta aagaaatgaa aaagtatatg aataaaaatg aaggtttaga
    12601 caataatccg tttaaagatg cattaaagaa cttgaattta tagataaaat cgaacaaagt
    12661 tgaatcagaa aaacgaaaag ttcgcttttg gtattgtttt ttattaagaa tgatattaaa
    12721 ctattaaggt attttaaaaa aaggagcatc cattcgtgaa aaactatttc cagttcgata
    12781 aatatggaac aaactttaaa agagaaatct taggcggtat cacaactttc ttatctatgg
    12841 cctatatttt agcagttaac ccgcaagttt taagtttagc aggtgttaaa ggcgtatcag
    12901 aagatatgaa aatggaccaa ggtgccattt ttgtagcgac tgcattagca gcatttgtag
    12961 gctcgctatt catggggcta atagctaaat atccaattgc gttagcacca ggtatgggat
    13021 taaatgcatt ctttgcattt actgttgtgt taaccatggg tattccttgg caagttggtt
    13081 taacaggcgt tttattctca ggtatattct ttgcgatatt aaccgtcaca gggtttaggg
    13141 aagttattat taatgcaatt ccttatcaaa tgaagatggc tgtttcagca ggtataggat
    13201 tattcattac atttgtaggt ttacaaagtt cgggtattat agttaaaaat gaatctacat
    13261 tagtaacatt gggacattta actgatccac cagtactttt ggcgatattc ggtatcgtta
    13321 ttacagttat tttatatgca attaaattgc cgggttctat ctttatcggt atgatcatca
    13381 cagcaatcgt tggtatgttc actggattga ttcaaatgcc atctggtatt gtgggtaaga
    13441 ttccaagtat tgaaccaacg ttcggtgctg catttgaagc gtttaaagac ccaagtcaat
    13501 tattaacgat acagttctta attgttattt taacgttctt atttattgat ttctttgata
    13561 cagctggtac attagtagct gttgctactc aagcaggtat tatgaaagat aataaattac
    13621 caagagctgg tagagcattg ttctcagatt ctttagctac gattgtagga tcagtttttg
    13681 gaacaactac aacaacttct tatatcgaat caacttcagg tgttgctgta ggtgctagaa
    13741 caggatttgc tagtatcgta acaggttgtt gtttcctatt agcgctgttc tttagtccgt
    13801 taatcgctgt tgtaacaagt gcagttacga caccagcctt agtggtggta ggtgtattaa
    13861 tggctgcaaa ctttgctgag ataaactgga aaagttttga agttgcagtg ccagccttca
    13921 ttacaatcat tatgatgcct ttatcatatt caattgcaac tggtattgcg tgtggattta
    13981 ttttctatcc aattacaatg cttatctcta aaaaacataa agaagtacat ccgataatgt
    14041 atgtattaat ggtactattc attctgtact tcatatttgt tcatggttaa atccagacac
    14101 aaaaaacgac tgccaatgat tggtagtcgt ttttttcttt gaagtaagga taaaataaga
    14161 agcgaaataa cataaagcga tattatgaat gtctttaaat atccgatata aatatacttc
    14221 ttttaaaatt gttggacgtg tttcttctat attatatacc taattaaaaa ggttttaaga
    14281 attattctct atgtccatct ttgtattgtg tgtaaatgac acgagcattt ttattaggta
    14341 tataggttgg cgctgataat acaactagga caaatagaat cattaggaaa aataaaatcc
    14401 aatgaatcaa aattcctaag aaggggataa tagctaaaaa actacctact ataccaaata
    14461 ttggaataat cgctataggt ctaatcgcat tttgtctatt cactaataat ataatagtaa
    14521 gaattagata aagtaatgca ttaaagagta aaggctgcca tccaaaactt aaaataaacg
    14581 ttgcacctaa aaaaggaatg ccatagaaaa actcacaaag tattaaaaag atacctataa
    14641 gtgctaaaga taattttggt ccatttttca tattcattca ccaccgttct tatgactaat
    14701 tatatagaat aattttcaat gataataata aaatgattca aggtacgaat ttacaataaa
    14761 gaaggaaata aaaaatatct cgaaaatagt tgaactgact aagtatataa agtaaaatat
    14821 aaaagtatgt gttagacaga tagaaaaatt atgtgaaaag ccttgattta tcgatgtttt
    14881 tcgtgtaata taactaacgt tggactttaa gaaaagcgat gaagcgaaag gttactgaca
    14941 cacccggccg ctttgccatg gcgctgtgta agatagtttt cgtggagaag tctatcacta
    15001 aatgtagacg aataaggagg gaaaattatg gcaaaacaaa aaatcagaat cagattaaaa
    15061 gcttatgatc accgcgtaat tgatcaatca gcagagaaga ttgtagaaac agcgaaacgt
    15121 tctggtgcag atgtttctgg accaattccg ttaccaactg agaaatcagt ttacacaatc
    15181 atccgtgccg tgcataagta taaagattca cgtgaacaat tcgaacaacg tacacacaaa
    15241 cgtttaatcg atattgtaaa cccaacacca aaaacagttg acgctttaat gggcttaaac
    15301 ttaccatctg gtgtagacat cgaaatcaaa ttataataga caattttagg aggtggactt
    15361 tcgatgacca aaggaatctt aggaagaaaa attgggatga cacaagtatt cggagaaaac
    15421 ggtgaattaa tccctgtaac agtagtagaa gctaaagaaa atgttgtatt acaaaagaaa
    15481 actgtagaag ttgatggata caacgcaatc caagttggat ttgaagacaa aaaagcatac
    15541 aaaaaagatg caaaatctaa taaatatgct aataaaccag ctgaaggtca cgctaaaaaa
    15601 gctgacgcag cacctaagcg cttcattcgt gaattccgca atgtagacgt ggatgcttac
    15661 gaagtaggtc aagaagtctc agtagatact tttgtagctg gcgacgttat tgacgtaaca
    15721 ggcgtatcaa aaggtaaagg tttccaaggt gcaattaaac gccacggaca atctcgtgga
    15781 cctatgtcac acggttctca tttccacaga gcaccaggtt ctgtaggtat ggcttcagat
    15841 gcttctagag tatttaaagg ccaaaaaatg ccaggacgta tgggtggaaa cactgtaact
    15901 gttcaaaact tagaagtagt tcaagttgac acagaaaaca aagttatctt agtaaaaggt
    15961 aacgtacctg gacctaaaaa aggtttagta gaaatcagaa cttcaattaa aaaaggtaat
    16021 aaataataag aagtgaaagg aggaaatgca taatggctaa ttatgatgtt ttaaaattag
    16081 acggaactaa atcaggttca atcgaattaa gcgatgcagt attcggtatt gagccaaata
    16141 atagcgtttt attcgaagct attaatttac aacgtgcttc attacgtcaa ggtacgcatg
    16201 ctgttaagaa tcgttcagca gtaagcggtg gcggacgtaa accatggaag caaaaaggaa
    16261 caggtcgtgc tcgtcaaggt acaatccgtg ctccacaatg gcgtggcggt ggtatcgtat
    16321 tcggaccaac tccaagaagt tatgcataca aaatgcctaa gaaaatgcgt cgtttagctt
    16381 tacgctcagc attatctttc aaagctcaag agaatggctt aactgtagtt gacgcattca
    16441 acttcgaagc tccaaaaact aaagaattca aaaatgtatt atctacatta gaacaaccta
    16501 aaaaagtatt agtagttact gaaaacgaag atgtaaatgt tgaattatca gcacgcaaca
    16561 tccctggcgt tcaagtgaca actgctcaag gtttaaatgt tttagatatc actaatgctg
    16621 acagcttagt aattactgaa gctgctgcta aaaaagttga ggaggtgctc ggataatgga
    16681 agcaagagat attcttaagc gccccgtaat cactgagaaa tcttctgaag caatggctga
    16741 agacaaatac actttcgacg ttgatactcg tgttaacaaa acacaagtaa aaatggcagt
    16801 tgaagaaatc ttcaacgtaa aagttgcaag tgttaatatc atgaattaca aacctaagaa
    16861 aaaacgtatg ggccgttacc aaggctatac aaacaaaaga agaaaagcga ttgtaactct
    16921 taaagaagga tcaatcgact tatttaacta ataaaacaat taccattaag gaggtaagcg
    16981 acaatggcta ttaaaaagta taagccaata acaaatggtc gtcgtaatat gacttcgtta
    17041 gatttcgcag aaatcacgaa aactacacct gaaaagtcat tattaaaacc gctaccgaaa
    17101 aaagcgggac gtaacaacca aggtaaattg actgtaagac accatggtgg tggacacaaa
    17161 cgtcaatacc gtgttatcga tttcaaacgt aacaaagatg gtatcaatgc aaaagttgat
    17221 tctattcaat atgatccaaa ccgctcagca aacatcgctt tagttgtata tgcagacggt
    17281 gaaaaacgat atatcattgc tcctaaagga ttagaagtag gtcaaatcgt tgaaagtggt
    17341 gctgaagctg acatcaaagt tggtaacgca ttaccattac aaaacattcc agttggtaca
    17401 gtagtacaca acatcgagct taaacctggt aaaggtggac aaatcgctcg ttcagctggt
    17461 gcaagtgctc aagtacttgg taaagaaggt aaatacgtat taatcagatt aagatctggt
    17521 gaagttcgta tgatcttatc tacttgccgt gctacaatcg gtcaagttgg taacctacaa
    17581 cacgaattag ttaacgttgg taaagccgga cgttcaagat ggaaaggtat ccgtccaaca
    17641 gttcgtggtt ctgtaatgaa ccctaacgat cacccacacg gtggtggtga aggtcgtgct
    17701 cctatcggta gaccatctcc aatgtcacca tggggtaaac ctacgcttgg taagaaaact
    17761 cgtcgtggta aaaaatcatc agacaaactt atcgttcgtg gacgtaagaa aaaataatat
    17821 aatcaactta tttgggtgtg cggcttaaag ctgcacgcac ataataagaa gggaggcgcc
    17881 caaatggctc gtagtattaa aaaaggacct ttcgtcgatg agcatttaat gaaaaaagtt
    17941 gaagctcaag aaggaagcga aaagaaacaa gtaatcaaaa catggtcacg tcgttctaca
    18001 attttcccta atttcatcgg acatactttt gcagtatacg acggacgtaa acacgtacct
    18061 gtatatgtaa ctgaagatat ggtaggtcat aaattaggtg agtttgctcc tactcgtaca
    18121 ttcaaaggac acgttgcaga cgacaagaaa acaagaagat aatatctatt aagtagagga
    18181 ggacatccta atggaagcaa aagcggttgc tagaacaata agaatcgcac ctcgtaaagt
    18241 aagactagtt cttgacttaa tcagaggtaa aaatgctgct gaagctattg caattttaaa
    18301 attaacaaac aaagcttcat caccagtaat tgaaaaagta ttaatgtccg ctttagctaa
    18361 tgctgaacat aactatgaca tgaacacaga tgaattagta gttaaagaag catatgctaa
    18421 cgaaggacca acattaaaac gtttccgtcc acgtgcgcaa ggtcgtgcaa gtgcgattaa
    18481 caaacgtaca agccacatta caatcgtcgt aagtgacggt aaagaagaag ctaaagaagc
    18541 ttaattaact tttaaggagg gaatactgtg ggtcaaaaaa ttaatccaat cggacttcgt
    18601 gttggtatta tccgtgattg ggaagctaaa tggtatgctg aaaaagactt cgcttcactt
    18661 ttacacgaag atttaaaaat ccgtaaattt attgataatg aattaaaaga agcatcagtt
    18721 tctcacgtag agattgaacg tgctgcaaac cgtatcaaca ttgcaattca tactggtaaa
    18781 cctggtatgg taattggtaa aggcggttca gaaatcgaaa aattacgcaa caaattaaat
    18841 gcgttaactg ataaaaaagt acacatcaac gtaattgaaa tcaaaaaagt tgatcttgac
    18901 gctcgtttag tagctgaaaa catcgcacgt caattagaaa accgtgcttc attccgtcgt
    18961 gtacaaaaac aagcaatcac tagagctatg aaacttggtg ctaaaggtat caaaactcaa
    19021 gtatctggtc gtttaggcgg agctgacatc gctcgtgctg aacaatattc agaaggaact
    19081 gttccacttc atacgttacg tgctgacatc gattatgcac acgctgaagc tgacactact
    19141 tacggtaaat taggcgttaa agtatggatt tatcgtggag aagttcttcc tactaagaac
    19201 actagtggag gaggaaaata ataatgttac taccaaaacg tgtaaaatat cgtcgtcaac
    19261 atcgtcctaa aacaactggt cgttctaaag gcggtaacta cgtaacattt ggtgagtttg
    19321 gtttacaagc tacaacaacg tcttggatca catctcgtca aatcgaatct gctcgtatag
    19381 caatgacacg ttacatgaaa cgtggcggga aagtttggat taaaatcttc ccacatacac
    19441 catatactaa aaaaccttta gaagtacgta tgggtgctgg taaaggtgcg gttgaaggct
    19501 ggatcgcagt tgttaaacca ggtagaattt tattcgaagt tgctggcgtt tctgaagaag
    19561 ttgcgcgtga agcactacgt ttagcaagtc acaaacttcc agtaaaaact aagtttgtaa
    19621 aacgtgagga attgggtggt gaaacaaatg aaagctaagg aaattagaga cttaaccact
    19681 tcagaaatcg aagaacaaat caaatcttca aaagaagagc tttttaacct acgctttcag
    19741 ttagctacag gtcaattaga agaaactgca cgtattcgta cagtaagaaa aacgattgca
    19801 cgtctaaaaa ctgttgctcg tgaaagagaa attgaacaaa gtaaggctaa tcaataatta
    19861 agtaagagga ggttacaaaa gtgagcgaaa gaaacgatcg taaagtttat gtaggtaaag
    19921 ttgtttcaga caaaatggac aagactatta cagtacttgt tgaaacttac aaaacacaca
    19981 aattatacgg taaacgagta aaatactcta aaaaatacaa aactcatgat gaaaacaatt
    20041 cagctaaatt aggagacatt gttaaaattc aagaaactcg tcctttatca gcaacaaaac
    20101 gttttcgttt agtagagatt gttgaagagt cagtaattat ttaatacaag tttagagata
    20161 agggaggttt aactaatgat ccaacaagaa acacgcttga aagtagcaga caactctggt
    20221 gctcgtgaag ttcttacaat caaagtatta ggtggatctg gtcgtaaaac agcaaacatc
    20281 ggcgatgtta tcgtatgtac tgttaaaaat gcaacaccag gtggcgttgt taaaaaaggt
    20341 gacgttgtca aagctgtaat cgtacgtact aagtcaggtg ttcgtcgtaa tgacggttca
    20401 tacatcaaat ttgatgaaaa tgcatgtgtt atcatccgtg atgacaaagg cccacgtggt
    20461 actcgtatct tcggacctgt tgctcgtgaa ttacgtgaag gtaacttcat gaaaatcgta
    20521 tcattagcac cagaagtact ttaattaata acaaccaata caaataagga ggtgcccaca
    20581 tgcatatcaa aaaaggtgac aacgttaaag ttatcgcagg taaagacaaa ggtaaagaag
    20641 gtaaagtaat tgctactcta cctaaaaaag accgtgtcgt tgtggaaggt gttaacatta
    20701 tgaaaaaaca ccaaaaacca actcaattaa atcctgaagg tggaatctta gaaacagagg
    20761 cagcaatcca tgtttctaat gtacaattat tggaccctaa aacaaacgaa ccaactcgtg
    20821 taggttacaa atttgttgat ggtaaaaaag ttcgtatcgc taaaaaatct ggcgaagaaa
    20881 ttaaatctaa taattaataa caagttgaaa ggaggatccc actttgaacc gtttaaaaga
    20941 aaagtttaac actgaagtta ctgaaaactt aatgaaaaaa ttcaattata gttcagtaat
    21001 ggaagtacca aaaatagata aaatcgttgt gaacatgggt gtaggtgacg cagtacaaaa
    21061 ttctaaagta ttagacaatg ctgttgaaga attagaattg atcactggtc aaaaaccatt
    21121 agtaactaaa gctaaaaaat caatcgcgac tttccgttta cgtgaaggta tgccaatcgg
    21181 tgcgaaagta acacttcgcg gtgaaagaat gtatgaattc ttagacaaat taatttcagt
    21241 atcattacca cgtgtacgtg acttccaagg tgtttctaaa aaagcatttg acggacgcgg
    21301 taactacact ttaggtgtta aagaacaatt aattttccca gaaatcgact atgataaagt
    21361 aagtaaagtt agaggaatgg atattgttat cgtaacgact gctaacactg atgaagaagc
    21421 tcgtgaattg ttagctaact tcggtatgcc attccgtaaa taattattta aaggaggcta
    21481 attaagtggc taaaacttca atggttgcta agcaacaaaa aaaacaaaaa tatgcagttc
    21541 gtgaatacac tcgttgtgaa cgttgtggcc gtccacattc tgtatatcgt aaatttaaat
    21601 tatgccgtat ttgtttccgt gaattagctt acaaaggcca aatccctggc gttcgtaaag
    21661 ctagctggta ataaaaaaga gtctgaaagg aggcaacaat caatgacaat gacagatcca
    21721 atcgcagata tgcttactcg tgtaagaaac gcaaacatgg tgcgtcacga gaagttagaa
    21781 ttacctgcat caaatattaa aaaagaaatt gctgaaatct taaagagtga aggtttcatt
    21841 aaaaatgttg aatacgtaga agatgataaa caaggtgtac ttcgtttatt cttaaaatat
    21901 ggtcaaaacg atgagcgtgt tatcacagga ttaaaacgta tttcaaaacc aggtttacgt
    21961 gtttatgcaa aagctagcga aatgcctaaa gtattaaatg gtttaggtat tgcattagta
    22021 tcaacttctg aaggtgtaat cactgacaaa gaagcaagaa aacgtaatgt tggtggagaa
    22081 attatcgcat acgtttggta ataaaaaata aggaggtgcc ataacatgag tcgtgttggt
    22141 aagaaaatta ttgacatccc tagtgacgta acagtaactt ttgatggaaa tcatgtaact
    22201 gttaaaggtc ctaaaggtga attatcaaga actttaaatg aaagaatgac attcaaacaa
    22261 gaagaaaaca caattgaagt tgtaagacca tctgattcta aagaagatag aacaaaccat
    22321 ggtacaactc gtgctttatt aaacaatatg gtacaaggtg tttctcaagg atacgtaaaa
    22381 gtacttgaac ttgttggtgt aggttaccgt gctcaaatgc aaggtaaaga cttaatcctt
    22441 aacgttggtt attctcaccc agtagaaatt aaagctgaag aaaacattac tttctcagtt
    22501 gagaaaaaca cagtcgttaa agttgaaggt atttcaaaag aacaagttgg agcattagca
    22561 tctaacatcc gttcagtaag acctccagag ccttacaaag gtaaaggtat tcgttaccaa
    22621 ggtgaatacg ttcgccgtaa agaaggtaaa actggtaaat aatagataaa ctctaaagaa
    22681 aggagtattg aaatgatcag taaaattgat aaaaataaag tgcgtttaaa aagacatgct
    22741 cgtgttcgta ctaacttatc aggtacagct gaaaagccac gtttaaacgt atatcgttca
    22801 aacaagcata tctacgctca aattattgat gataataaag gcgtaacatt agctcaagct
    22861 tcttcaaaag acagcgacat tgctactaca gcaactaaag ttgaattagc aactaaagtc
    22921 ggtgaagcaa ttgctaaaaa agctgctgac aaaggcatta aagaaatcgt atttgaccgt
    22981 ggaggatatt tatatcacgg acgtgttaaa gcattagctg aagcagcaag agaaagcgga
    23041 ttagaatttt aatttaaagg agggacaaat acatggctcg tagagaagaa gagacgaaag
    23101 aatttgaaga acgcgttgtt acaatcaacc gtgtagcaaa agttgtaaaa ggtggtcgtc
    23161 gtttccgttt cactgcatta gttgtagttg gagacaaaaa tggtcgtgta ggtttcggta
    23221 ctggtaaagc tcaagaggta ccagaagcaa tcaaaaaagc tgttgaagca gctaaaaaag
    23281 atttagtagt tgttccacgt gttgaaggta caactccaca cacaattact ggccgttacg
    23341 gttcaggaag cgtatttatg aaaccggctg cacctggtac aggagttatc gctggtggtc
    23401 ctgttcgtgc cgtacttgaa ttagcaggta tcactgatat cttaagtaaa tcattaggat
    23461 caaacacacc aatcaacatg gttcgtgcta caatcgatgg tttacaaaac cttaaaaatg
    23521 ctgaagatgt tgcgaaatta cgtggcaaaa cagtagaaga attatacaat taaggaggga
    23581 aaactagtta tggctaaatt acaaattacc ctcactcgta gtgttattgg tcgtcctgaa
    23641 acacaacgta aaactgttga agctttaggt cttaaaaaga ctaacagttc agtagttgtt
    23701 gaagataacc ctgctattcg tgggcaaatc aacaaagtta agcacttagt aacagtagaa
    23761 gaaaaataat caaggaggtg ccgaaatgaa attacatgag ttaaaaccgg cagaaggttc
    23821 acgtaaagaa cgcaatcgtg ttggacgtgg tgttgcgaca ggtaacggta aaacaagtgg
    23881 tcgcggacac aaaggtcaaa aagctcgttc aggcggtggt gtaagaccag gatttgaagg
    23941 tggtcaatta ccattattcc gtcgtttacc aaaacgtggt tttactaaca taaatcgtaa
    24001 agaatatgct attgttaact tagaccaact taataaattt gaagatggta ctgaagtaac
    24061 tccagcttta ttagtagaat ctggtgttgt taagaatgaa aaatctggta tcaaaatact
    24121 aggtaatggt tcacttgata agaaattgac agtgaaagct cataaattct cagcttcagc
    24181 agcagaagct attgatgcta aaggtggagc acacgaggtg atctaatgat tcaaaccctt
    24241 gtgaacttct ttagaacaaa agaggttcgt aacaagattt tcttcacact agcaatgtta
    24301 gtaattttta aaatagggac ttatatacca gctccaggag taaatcctgc agcttttgat
    24361 aatccccaag gttctcaagg tgccactgag ttattaaata cttttggtgg cggagccttg
    24421 aaacgatttt ctatttttgc aatgggtatt gtaccctaca tcactgcatc aatcgtaatg
    24481 caattattac aaatggatat tgtccctaaa ttctcagaat gggcaaaaca aggtgaagta
    24541 ggtagaagaa agttaaataa cgttactcgt tatttagcaa tttctttagc atttatccaa
    24601 tctataggta tggcattcca atttaataat tatctcaaag gtgcgctgat tatcaatcag
    24661 tcaattatga gttatttatt aatagcacta gttttgacag caggaactgc tttcttaata
    24721 tggcttggtg atcaaatcac tcagttcggt gttggtaatg gtatttctat tatcatattt
    24781 gcgggtattt tatcaacgtt gccagccagt ctaattcaat ttggacaaac tgcgttcgtt
    24841 gggcaagaag atacatcatt agcatggttg aaagtattag gacttttagt gtcactgata
    24901 ctattaactg taggtgctat ttatgtactt gaagccgttc gtaaaattcc gattcaatat
    24961 gctaagaaac aaactgctca aagattaggt tcacaggcaa cttatctacc tttaaaagtg
    25021 aactcagccg gtgttattcc tgtaatcttt gcaatggcgt tcttcttact gcctagaaca
    25081 ttaacgttgt tctatccaga taaagaatgg gcacaaaaca ttgcgaatgc tgcaaatcct
    25141 tcaagtaatg ttggtatggt tgtatatatt gtactaatta tactattcac atacttctat
    25201 gcatttgtac aagttaatcc ggaaaaaatg gctgataacc ttaagaaaca aggtagctat
    25261 gttccaggta ttagacctgg tgaacaaacc aaaaaatata ttactaaagt tttatatcgt
    25321 ttaacttttg taggttctat cttcttagcc gttatttcaa ttctaccgat attagcaact
    25381 aagtttatgg gattaccaca atcaattcag attggtggta caagtttact tatcgttatt
    25441 ggtgtagcga ttgaaacaat gaaatcttta gaagctcaag tatctcaaaa agaatataaa
    25501 ggctttggtg gtagataatt ttaggagggc acttatgaat atcattttga tgggtttacc
    25561 tggcgcaggt aaaggaactc aagcaagtga aattgtcaag aaattcccaa taccccacat
    25621 ttcaactggt gacatgttca gaaaagctat aaaagaagaa actgaattag gtaaagaagc
    25681 taagtcttat atggaccgtg gcgaattagt tcctgatgaa gtgactgtag gtatcgttaa
    25741 ggaaagaatt tctgaagacg atgcaaaaaa aggcttttta ttagatggct tcccaagaac
    25801 aatcgagcaa gctgaggcat taaataatat tatgtctgag cttgacagaa acattgatgc
    25861 tgtcatcaat atcgaagttc cggaagaaga attaatgaac cgtcttacag gtcgtcgaat
    25921 ctgtgagtca tgtggtacaa cgtatcatct tgtatttaat cctccgaagg tcgaaggtat
    25981 ttgtgatatc gatggtggta aattgtatca acgagaagat gataatcctg aaacggtagc
    26041 taatcgtttg agtgttaata ttaaacaatc taagcctatt ttagatttct atgatcaaaa
    26101 aggtgtattg aaaaatattg atggttcaaa agatattagc gacgttacca aagatgtcat
    26161 tgatatttta gatcatttgt aatagatcaa cttttagcgg tctattttaa gtgaggcatg
    26221 atttaatcat attagataga tgagagtggc aattaacgat aattaattcc cgtaattttt
    26281 gtttaactta taaaattaat cactgataga ttcgtaacaa taaagttgtc tattgacgat
    26341 taccttacta ttgcaaaaag ggggaagtta atcaatggct aaacaagatg taattgaatt
    26401 agaaggtact gtattagata ctttaccgaa cgcaatgttt aaagtagaat tagaaaatgg
    26461 tcatgagatt ttagctcacg taagtggtaa aatcagaatg aattacattc gtattctacc
    26521 tggcgacaaa gtaactgttg agatgtctcc gtacgattta acacgcggaa gaattactta
    26581 tcgttataaa taatcgtcac tccataatat agggaggtat aaaaatgaaa gtaagaccat
    26641 cagtaaaacc tatttgcgaa aaatgtaaag tcattaaacg taaaggtaaa gtaatggtaa
    26701 tttgtgaaaa tccaaaacac aaacaaagac aaggttaata aaagagaggt gtaaattaat
    26761 atggcacgta ttgcaggagt agatattcca cgtgaaaaac gcgtagttat ctcattaact
    26821 tatatatacg gtatcggtac gtcaactgct caaaaaattc ttgaagaagc taacgtatca
    26881 gctgatactc gtgtgaaaga tttaactgat gacgaattag gtcgcatccg tgaagttgta
    26941 gacggttata aagtcgaagg tgacttacgt cgtgaaacta acttaaatat caaacgttta
    27001 atggaaattt catcataccg tggtatccgt caccgtcgtg gtttaccagt tcgtggtcaa
    27061 aaaacgaaaa acaacgcgcg tactcgtaaa ggaccagtta aaacggtagc taacaagaaa
    27121 aaataatagg taaaggaggc aaattttaaa tggcacgtaa acaagtatct cgtaaacgta
    27181 gagtgaaaaa gaatattgaa aatggtgtag cacacatccg ttcaacattc aacaacacta
    27241 ttgtaactat cactgatgag ttcggtaatg ctttatcatg gtcatcagct ggtgcattag
    27301 gattcaaagg atctaaaaaa tcaacaccat ttgcagcaca aatggcttct gaaactgcat
    27361 ctaaatcagc tatggagcat ggtttaaaaa cagttgaagt aacagttaaa ggacctggtc
    27421 caggtcgtga atcagctatt cgtgcattac aatctgcagg tttagaagta actgcgatca
    27481 gagacgttac tccagtacct cataacggtt gtcgtccacc aaaacgtcgt cgtgtataat
    27541 ttatgatggt attgttacag gtcactgagc aaacatttta aattaagtcg acgtatataa
    27601 ggaggatatt taaatgatag aaatcgaaaa acctagaatt gagacaattg aaattagtga
    27661 agatgctaaa ttcggtaagt tcgttgttga accactagaa cgtggctacg gtactacact
    27721 aggaaactcc ttacgtcgta tcctactatc ttcattacca ggtgcagccg ttaagtatat
    27781 tgaaattgag ggagttttac atgaattctc agcagtagac aatgtagttg aagatgtttc
    27841 tacaattatt atgaacatta aacaattagc attgaaaatt tactctgaag aagataaaac
    27901 tttagaaatt gatgtacgtg atgaaggcga agtaacagca agcgacatta cacatgatag
    27961 tgatgttgaa attttaaacc cagagcttaa aattgcaaca gtatctaaag gtggtcactt
    28021 aaaaattcgt ctagttgcta acaagggtag aggttacgca ttagcagaac aaaataatac
    28081 tagtgattta ccaattggtg taatccctgt tgattcattg tattcacctg ttgaacgtgt
    28141 gaactatact gttgaaaata cacgtgtagg tcaaagcagt gattttgata aattaacatt
    28201 ggatgtttgg actaatggtt caatcacacc acaagaatca gtttcattag cagcaaaaat
    28261 aatgactgaa cacttgaata tcttcgttgg tcttactgat gaagcgcaaa acgctgaaat
    28321 catgattgaa aaagaagaag atcaaaaaga aaaagtatta gaaatgtcta ttgaagaatt
    28381 agacttatct gtacgttcat ataactgctt aaaacgcgca ggaatcaatt ctgttcaaga
    28441 gttagctgac aaatctgaag ctgacatgat gaaagtgcgt aatttaggtc gtaaatcttt
    28501 agaagaagtt aaatacaaat tagaagattt aggattagga ttaagaaaag aagattgata
    28561 aaggaggtta actcatgggt tacagaaaat taggtcgtac ttctgatcaa cgtaaagcta
    28621 tgttacgtga cttagctaca tcacttatta ttagtgaacg tattgaaact acagaagctc
    28681 gtgcaaaaga agttcgcagt gttgttgaga aattaatcac tttaggtaaa aaaggagatt
    28741 tagcttctcg tcgtaatgca gctaaaactt tacgtaatgt tgaaatctta aacgaagatg
    28801 aaactacaca aactgcactt caaaaattat ttggtgaaat cgcagagcgt tacacagaac
    28861 gtcaaggtgg ttacactcgt atccttaaac aaggccctcg tcgcggtgac ggtgctgaat
    28921 cagtaattat cgaattagta taagaattta tataaataca ctaactactt ttatataaag
    28981 caaatgagtg caacgataat gtttgccaca tacaaatatt gtctagctca gagtacccca
    29041 tcaactaaat aaagatttaa agcgtgaact caacaaactt atgatggggt gcgcgctttt
    29101 ttattgaata aaatatcgga cttatgaaca gaaatgtttt taagtccgat ttctttatat
    29161 ttactttttt gagtgcgaat ctaaaaatat gatgaagcta gtttgatcaa tttcactaga
    29221 actgaatgaa gtgaaagtat tatcgttgaa tcaaacgcga ccacccctat attagtaaat
    29281 catttatttt attaacgttt atacataatt gttagagagg aataaaagtc cttatcatta
    29341 gttgttaaac aacactttaa agactagaaa tgagtcagaa aatactagta atatatagag
    29401 aacccagtca atgtcatata cagcgattta taaatattgt aaaatatgat agatacacga
    29461 atcgtaaagg agagagacaa gtggaggata agaattcagt tattgtattt aaaaatgttt
    29521 catttcaata tcaaagtgat gcatccttca cattgaaaga tgtttctttt aatataccta
    29581 aaggtcagtg gacatctatt gttggtcata acggttctgg aaaatctaca attgccaagt
    29641 taatgattgg catagagaaa gttaaatctg gagaaatttt ttataataat caagctataa
    29701 ctgatgataa ttttgaaaag ttaagaaaag acataggaat tgtatttcag aatccggata
    29761 atcaatttgt tggttcaatt gtaaaatacg atgtggcatt tggactcgaa aatcatgcgg
    29821 ttccatatga cgaaatgcat agaagagtca gcgaagcact taaacaagtt gatatgttag
    29881 aacgtgcaga ttatgaacct aatgcattat cggggggaca gaagcagcgt gtggctatag
    29941 caagtgtatt agcacttaac ccctctgtca ttatattaga tgaggcgact tctatgttag
    30001 atcctgatgc acgtcaaaat ttattggatt tagtgagaaa agttaaatca gaacataata
    30061 ttacaatcat ttctattacg catgatttat ctgaggcgat ggaagcagat catgttatcg
    30121 ttatgaataa agggactgtc tataaagaag gcacagcgac tgaaattttc gacaatgcag
    30181 aagagttaac aagaataggt ctagatttgc cattcccaat caaaataaat caaatgctgg
    30241 gacaccaaac atcattctta acttatgaag ggctggtgga tcaactatga ctatacggtt
    30301 tgacaatgta agttatacct atcaaaaagg gacaccatat cagcatcaag ctattcatga
    30361 tgttaataca gaatttgaac aaggtaaata ttacgccatc gttggacaaa cgggtagtgg
    30421 taaatcaacg ttgatacaaa atattaatgc gctgttaaag ccgactactg ggacagttac
    30481 agttgatgac attactatta cacataagac caaagataaa tatattagac ctgtaagaaa
    30541 aagaattgga atggtatttc aatttcccga atctcaatta tttgaggaca cagtagagcg
    30601 tgaaatgata tttggaccta aaaactttaa aatgaattta gatgaagcca aaaactatgc
    30661 ccatcgtctg ttgatggatt taggcttttc aagagatgta atgtcacaat caccatttca
    30721 aatgtcaggt ggacaaatgc gtaaaatagc gattgtatcg atattggcaa tgaatcctga
    30781 tattatcgtg gttgatgaac ctacagcagg acttgatcca caaagtaaac gacaagtaat
    30841 gagattacta aagtcactac aaacagatga aaataaggca attatcctaa tttcacatga
    30901 tatgaatgaa gtcgcgcgtt atgcagatga agtcatcgtt atgaaagaag gtagtatagt
    30961 gtcgcaaaca tcaccaaaag agctcttcaa agataaaaaa aaattggcgg attggcatat
    31021 tggtttgcca gaaattgttc agttacaata tgactttgaa caaaaatatc aaacaaaatt
    31081 aaaagatatt gccttaactg aagaagcatt tgtaagcttg tataaggagt ggcaacatga
    31141 aaaataaatt gattataggg cgatatttac caattaattc ttttgttcat catcttgatc
    31201 caagagcaaa gttaatgttt gtttttcttt ttattatatt aatatttttc tgtcattcac
    31261 cattaacata tctttgggtg tttgcactta tcttattcat tatgagatta gcaaaaattc
    31321 aattatggtt cctaatcaaa ggtttaacac caatattttt ctttttaatc ttcacattaa
    31381 tgatgcatat atttttaact aaaggtggat atgtattagt tgaatggcat ggcatcacga
    31441 ttgaaactaa tggtatttta gaagggctat atatatcact gcgtttaatt gggattgtaa
    31501 tgattgcaac aattatgaca ctatctacaa gtccaattga tttaacagat gcgtttgaaa
    31561 gattacttgc accactaaag atgtttaaat taccagttca tcaattaagt atgataatgt
    31621 ccattgcgtt acgattcatc ccgacgttaa tggatgagtt agataaaatc attttggcgc
    31681 aaaagtcgcg tggttctgaa ataagttcag ggaacattgc aacacgtatt aaatcattta
    31741 ttccattact agtgccacta ttcatctcag ctttccaacg cgccgaagaa ttagcggtcg
    31801 caatggaagt taggggttat gatgccaatg ttaagagaac gagctacaga cagcttaaat
    31861 ggcaattgag agatacgata tctttaacca tgattatccc aattgcaatt attttattcg
    31921 tattaaaata ttcaggagtg taaattcatg cgtatattag tagaaattgc gtatcaagga
    31981 aataattttc taggttttca aattcaacaa aatggtcgaa cggtacagca acaatttgaa
    32041 aagctattac aacgcatgca taaaagacat gtgagaatcc atccttcaag taggactgat
    32101 agaggtgtac atgcgataca gcaatacttt catttcgaca cagagctgaa tatacctatg
    32161 tcacaatggc aatatgcaat gaaccgaacg ttacctgatg atatttatgt caacaatgta
    32221 gttacagtcg atgacgattt tcattgtcgt tatgattgtg taggtaaacg ttatcgctac
    32281 aaagtatatc aagcacaaca ccgcgatcca tttcaaagtg gtttgaaaac atttattcct
    32341 gaaacgttgg atttaggtaa aatgaacaga gcggcgcaac aattcatagg tacacatgat
    32401 tttacgggat tttgttcgca aaaaactgaa gtagaaagca aagttagaac attataccaa
    32461 agtgaaatcg taaagacaga tgatggcttt gattatattg tcactggatc tggattttta
    32521 tataacatgg tgcgtgtctt agttgcattt ttaatagaag ttggaaaagg gcgacatgaa
    32581 gtatctgatg taccaaaact tcttgaatca aagaatcgta agaatgtacc tttcactgca
    32641 ccggctgaag gattatattt agagaaaatt tatttagatg aaaatgagtt attaaaagac
    32701 tttggtaacg atatcaaaat acatcgtaaa aaatcattac aaaatgactg attgccattg
    32761 acaaaaagct tgtgaaatta taagattatt aacggtattg ttttatatcc accccacgat
    32821 aagccccgga aacttattgt gttacaagat atataagcag aaacgaacaa cagttaacaa
    32881 aataaatgaa attaaacgtt ttaaaaatga aacaaatgaa atcatctatt aggttatgaa
    32941 actgtttata gcttgaatag aagcatttat tttttaggag gacaattatt atgcgtcaaa
    33001 catttatggc aaatgaatca aacattgagc gcaaatggta tgttatcgat gctgaaggcc
    33061 aaacattagg tcgtttatca tcagaagtag catctatctt acgcggtaaa aataaagtaa
    33121 cttacacacc acacgttgat actggtgatt atgtaatcgt tattaatgca tcaaaaatcg
    33181 aatttactgg taacaaagaa actgacaaag tttactaccg tcactcaaat cacccaggtg
    33241 gtatcaaatc aatcactgct ggtgaattaa gaagaactaa cccagaacgt ttaattgaaa
    33301 actcaattaa aggtatgtta ccaagcactc gtttaggcga aaaacaaggt aaaaaattat
    33361 ttgtatatgg tggcgctgaa catccacacg ctgcacaaca accagaaaac tacgaattac
    33421 gtggttaatt agaaggagga aatgactttg gcacaagttg aatatagagg cacaggccgt
    33481 cgtaaaaact cagtagcacg tgtacgttta gtaccaggtg aaggtaacat cacagttaat
    33541 aaccgtgacg tacgcgaata cttaccattc gaatcattaa ttttagactt aaaccaacca
    33601 tttgatgtaa ctgaaactaa aggtaactat gatgttttag ttaacgttca tggtggtggt
    33661 ttcactggac aagctcaagc tatccgtcac ggaatcgctc gtgcattatt agaagcagat
    33721 cctgaataca gaggttcttt aaaacgcgct ggattactta ctcgtgaccc acgtatgaaa
    33781 gaacgtaaaa aaccag
//
LOCUS       DANRDI010000012        33381 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.012, whole genome shotgun sequence.
ACCESSION   DANRDI010000012 DANRDI010000000
VERSION     DANRDI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33381
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.012"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(75..1667)
                     /locus_tag="PZ377_000417"
     CDS             complement(75..1667)
                     /locus_tag="PZ377_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6357309.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            1624..3102
                     /locus_tag="PZ377_000418"
     CDS             1624..3102
                     /locus_tag="PZ377_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6357310.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFLLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            3108..4271
                     /locus_tag="PZ377_000419"
     CDS             3108..4271
                     /locus_tag="PZ377_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6357311.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            4288..6063
                     /locus_tag="PZ377_000420"
     CDS             4288..6063
                     /locus_tag="PZ377_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDM6357312.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            6101..8107
                     /gene="ggt"
                     /locus_tag="PZ377_000421"
     CDS             6101..8107
                     /gene="ggt"
                     /locus_tag="PZ377_000421"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDM6357313.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(8405..9177)
                     /locus_tag="PZ377_000422"
                     /pseudo
     CDS             complement(8405..9177)
                     /locus_tag="PZ377_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            complement(9367..9993)
                     /locus_tag="PZ377_000423"
     CDS             complement(9367..9993)
                     /locus_tag="PZ377_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDM6357314.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            10202..10780
                     /locus_tag="PZ377_000424"
     CDS             10202..10780
                     /locus_tag="PZ377_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDM6357315.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            11163..12260
                     /gene="ugpC"
                     /locus_tag="PZ377_000425"
     CDS             11163..12260
                     /gene="ugpC"
                     /locus_tag="PZ377_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDM6357316.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            12273..13544
                     /locus_tag="PZ377_000426"
     CDS             12273..13544
                     /locus_tag="PZ377_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDM6357317.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            13547..14815
                     /locus_tag="PZ377_000427"
     CDS             13547..14815
                     /locus_tag="PZ377_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDM6357318.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            14817..15656
                     /locus_tag="PZ377_000428"
     CDS             14817..15656
                     /locus_tag="PZ377_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDM6357319.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQIFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            15730..16806
                     /locus_tag="PZ377_000429"
     CDS             15730..16806
                     /locus_tag="PZ377_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDM6357320.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            16831..17871
                     /locus_tag="PZ377_000430"
     CDS             16831..17871
                     /locus_tag="PZ377_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDM6357321.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            17926..18894
                     /locus_tag="PZ377_000431"
     CDS             17926..18894
                     /locus_tag="PZ377_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDM6357322.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(19256..19750)
                     /locus_tag="PZ377_000432"
     CDS             complement(19256..19750)
                     /locus_tag="PZ377_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDM6357323.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            19983..21362
                     /gene="uhpT"
                     /locus_tag="PZ377_000433"
     CDS             19983..21362
                     /gene="uhpT"
                     /locus_tag="PZ377_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDM6357324.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(21721..22479)
                     /locus_tag="PZ377_000434"
     CDS             complement(21721..22479)
                     /locus_tag="PZ377_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6357325.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(22472..24028)
                     /locus_tag="PZ377_000435"
     CDS             complement(22472..24028)
                     /locus_tag="PZ377_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDM6357326.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(24025..24993)
                     /locus_tag="PZ377_000436"
     CDS             complement(24025..24993)
                     /locus_tag="PZ377_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDM6357327.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            25581..27830
                     /gene="pflB"
                     /locus_tag="PZ377_000437"
     CDS             25581..27830
                     /gene="pflB"
                     /locus_tag="PZ377_000437"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDM6357328.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            27853..28608
                     /gene="pflA"
                     /locus_tag="PZ377_000438"
     CDS             27853..28608
                     /gene="pflA"
                     /locus_tag="PZ377_000438"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDM6357329.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            28944..30707
                     /locus_tag="PZ377_000439"
     CDS             28944..30707
                     /locus_tag="PZ377_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDM6357330.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(30871..31215)
                     /locus_tag="PZ377_000440"
     CDS             complement(30871..31215)
                     /locus_tag="PZ377_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDM6357331.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            31405..33234
                     /gene="coa"
                     /locus_tag="PZ377_000441"
     CDS             31405..33234
                     /gene="coa"
                     /locus_tag="PZ377_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDM6357332.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNV
                     TTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
BASE COUNT        11277 a         5138 c         6259 g        10707 t
ORIGIN      
        1 tctaacataa cacatcagaa gtcgcgcaat ttatttcaga ccatacagtg ataaagttgc
       61 acaacgcatg acttttattt agcaataact gctacttctg aaataagttg ctttgcatag
      121 tctgactgcg gatgtttgat aatatcttct gtgttattca gttcaacgat ttcgccattt
      181 ttcataactg caacgcgatc acatatttca ttgataacac ccatgtcatg tgtgatgaat
      241 aaataagtga tgccgaagtc taactgtaat tgttttaata actcgatgat atctttttga
      301 attgaaacgt ctaaagcgga cactgcctcg tcgcaaacaa tcactttagg ttcaacagca
      361 agtgctctcg cgatacttac acgctgacgt tgcccaccag ataattcgtg tggatagcga
      421 tataagaaag tttgatctag gccaaccttt tctaacaacg atacgacagt tttaataatg
      481 tcatcattat ctttgacttt cccatgaatg attagtggtc gtttaatcac atcaatgact
      541 ttaaatcttg gattaataga tgcgaatgga tcttgaaaaa tcatttgtat ctcttgtcgt
      601 aaagatttca attcatcatc tttaaataaa cttaatggta attcgttata ccaaataaag
      661 ccttctgaca cttcctttag accgacgacc gtcttagcta atgtcgattt ccctgaccct
      721 gattcaccga caatgcctaa tgtttcgcct tttctaatag ccaagttaat atcattaact
      781 gctcggtata ggctgccact cggtgatgtg taatccacgc tcacgcgatc gaattttaat
      841 aaaatatcat tgtttaacgg tcttggcgga cgcgtttgat gaatatcagg aatcgcatct
      901 attaagcgtt ttgtataggt atgttgtggc gatttaaaaa tactttcaac cgtgccactt
      961 tcaacgacac ttccatcttt cattacaatc acatcgtcgc aaaattgata cacagcgcct
     1021 aaatcgtgag tgataaaaat aatagatgtt tctgtgtact cataaaggga cttcattaac
     1081 tgcagtaatt gattttgtgt actggcatct aatgccgttg ttggttcatc tgcgattaaa
     1141 atttgtggct ttaaaatcaa tgccattgct atcatgacac gttgacgcat accaccagaa
     1201 agttcatgtg gataagcatc aaattgtcga gttgcatgtt ttatacctac tttttctaaa
     1261 atgtctattg tcatcgactt tgcttcagat ttagatacac gtttatgttg aaatattact
     1321 tctgtaattt gtttgccaat cgttaatctt ggattcaacg aagagagtgg atcttgaaaa
     1381 atcattgaaa tatccttacc tcgaatttgt tgtaacgctg aagttgataa attatttaac
     1441 gattgcccat taaaaataat ttctcctgtt aatgtgtgat ctggataatc tggtagtagc
     1501 cctaaaatag atttagcggt aatacttttt cctgatcctg attcaccaac aatacctagg
     1561 atatgttttt ttcgtaattc gaaagagacg ttttttaccg cttgaactgt agtttcatca
     1621 taattgaatt gtacattcag actgttgact tctaataaat ttgacatgat gttgtgcctc
     1681 cctattcaca aatgtgtaaa tttattctat acttttgtat atcatagtaa tctactcaag
     1741 attattttac aaggagtgat tatcataatt acttatttag aatcttttaa gtcgttgtat
     1801 cacaaggcat tttacaagtt tttattatcg gtattaagct tgccaatatt tttatatgca
     1861 gtgataaagt ttttcttttc tgctaagagg aaaaattttt acgctaataa ttctgaaatt
     1921 tcagaaattg aacaggcgtt acatcaaaaa tataaatatt tatcgcagca aaagtcatcc
     1981 acacaaatac ataaagaagc attaaaaata ttcaaggcac aaagttctaa tacgagttca
     2041 aagaatattg aacaagcaca tttttcaaca tactttgaaa atgtattatt tcataagttc
     2101 atcatgatca aagtgatatt ggccttgccg atgttcatct tattgacttt ttatttacag
     2161 ccattagtta gatatatttt tgaacgaatt gtcatggctg tgattgtcat cattggtgtt
     2221 attgtcagtg tgtttaccat tctgtatttt tcaccgcttg atgcggctta tagcatactg
     2281 ggacaaaatg caacaaaggc acagatacat caattcaatg tattacatca tcttaacgaa
     2341 ccttatttta ttcaattgtg ggataccatc aagggtgttt ttacctttga cttaggtacg
     2401 acttacaaag ggaatgaggt tgtgactaaa gcagttggcg aaagaattcc aattacaata
     2461 attgtcgcag tattagcgct aatggtggca ttaattattg caataccaat tggtattatc
     2521 agtgcgatga agcgaaatag ttggcttgat atcacgttaa tgataattgc attaattggt
     2581 ttatctattc caagtttctg gcaagggcta ttattcattt tagcgttctc attgaaattg
     2641 gatattttgc caccatctta tatgccagaa catccaatat cgttgatttt acctgtactt
     2701 gtcattggaa caagtattgc tgcttctatc acgcgtatga caaggtcttc tgtacttgaa
     2761 gtaatgcgca gcgattatgt tttaactgct tatgcaaaag gattatcgac gacacaagtt
     2821 gttattaaac atattttgaa aaatgccatt attccaattg taacgttagt tggtcttcta
     2881 gtggcagagt tactaggcgg ttcagcagtg acggaacaag tatttaacat taatggtatc
     2941 gggcgttata tcgtccaaaa acaactaata cctgatattc cagcagtcat gggtggggtc
     3001 gtatatatat caattgtaat atctttagca aacttaatta ttgatatatt ttatgcttta
     3061 atcgatccaa aattacgtag tgaaatcaac gaaaggaagt gaggcatatg gcacaactta
     3121 attcaaagat agcttcctta aaattattcg caagttacgc catagcaact tatattttag
     3181 ttatattaac gagtgcatta aatcttttta aaggttatgt ggccgatacg ttctatattg
     3241 ctgaaacatt gctaatcgtt ttaaccatca ttttaattat tattttaaca acggaacaaa
     3301 catggaagca tcatgaccta tggcgacgta tcgtcgaagt gttgttattg ttgatgacat
     3361 taacaggcaa cgtatttaca ttattaatgt ttgtaagtat tagacgttac caacgtacat
     3421 cgcaaataca tagttataac gggtgggaat cgtttatacg aaaaactact agacatcgta
     3481 ttgcgattat cgggttactt attttagtct acatgctgac attatcaatt gtgtcacaat
     3541 ttacatttga tacgacattg gctactaaaa atcagttcaa tgcactgtta catggaccga
     3601 gtctagccta tccgtttggt actgatgatt tcggtagaga cttatttaca cgcgtagttg
     3661 taggaacgaa gctgacattt tcaatttcaa ttatttcagt agttattgca gttatttttg
     3721 gtgtgttact aggcactatc gcaggttatt ttaatcatat tgataattta ataatgcgaa
     3781 ttttagatgt agtgtttgca attccatcat tattgttagc ggtggcaatt attgcatcat
     3841 ttggagcaag tattccaaat ttaattattg ctttaagtat cggtaatata ccatcatttg
     3901 cacggacaat gcgtgccagt gttttagaaa ttaaacgcat ggaatatgta gatgcagcac
     3961 gtatcactgg tgaaaacact tggaatatca tatggcgtta tattttaccg aatgcgattg
     4021 cgcctatgat tgtacgtttt tcattaaata taggtgtggt tgtattaaca acaagtagtt
     4081 taagtttcct aggacttggt gttgcacctg atgtagctga atggggcaac attttacgta
     4141 ccggtagtaa ctacttggaa acgcacagta atttagctat tgtacctggt gtttgtatta
     4201 tgttcgtcgt tttagcattt aattttatag gtgatgcagt gcgtgatgca ctagatccaa
     4261 gaattcatta aaaaggtagg gatagatgtg aagaaaatca ttagtatcgc aattatagtt
     4321 ttagcgttgg tattaagtgg ttgtggtgtc cctacgaaat cagaagtggc tcaaaagtca
     4381 tcgaaagttg aagtgaaagg cgagcgacca acaatacatt tcctaggaca agcaagttat
     4441 gaaaatgata tgaatatcgt taaagatcaa ttggaaaatg caggatttaa cgtgaagatg
     4501 aatatccaac cagattatgg tagctatcgt acacaacgtc aagccggcaa ttatgatatc
     4561 caaattgatg actggatgac agtgtttggt gacccgaact atgctatgac ggcattattt
     4621 agttctacag gatcaaatag tttattgaaa gataaacatg tagaccagtt gttaaataaa
     4681 gcttctactc aaaatgaagc agatgttaaa caaacatata agcaaattga agatgaagtt
     4741 gtatttgata aagggtatat ggcgccttta tatggatcaa aaaagaattt agtatatgac
     4801 aataaagtgt tagataaaaa tagtgttgga ttgccaaatt cacgtgcatt aatatggcaa
     4861 caatttgatt acaacaatag tagagaacga gatacgcggc cacttgtgat gacacaacaa
     4921 gatggtgaaa ttcctacatt ggatccaata cgttcaattg cgccgtcagt atattcaatt
     4981 aatatgaata tgtacacaag gttattatta ttagatgaaa atgatcattt aacaacgaaa
     5041 ggttcgttaa gtcatgatta tgctgtgaat aaggacaata aagcatttta tttcttgtta
     5101 agagatgatg attattttgc gaaagtggtc aatggacaag cacgtaatac tggagagcgt
     5161 gtatcggctg aagatgttaa gttttcttta gatagagcac gtgataaaaa gtctgtgcct
     5221 aacaataata cttacaatat gcacaaacat ataaatgaca tcaagatatt aaaagatgag
     5281 gacatcgatc agttgcgtaa agagaaagac aaggacgata aatcaatcta tgataagttg
     5341 attaaagctt ataacgtcaa atcgttaacg acagatggtc aaaaagtaaa taataaagac
     5401 ggtatttatc aaattgttaa aattacgaca gatcaatcga tgcctcgaga ggtaaattac
     5461 ttaacacact cttcggcagg cattttatct aaaaaatttg ttaatcaagt aaatcaagaa
     5521 tatccaaaag gatatgggga tagcagtaca attcctgcaa attcagatgg gaaaaatgcg
     5581 ctgtatgcaa gtggcgcata cattatgaca cagaaaaatg catatcaagc aacgtttcaa
     5641 cgtaatccag gattcaacga aacagaaaaa ggtagttatg gaccagctaa aattaaaaat
     5701 attacattga agtttaatgg tgacccgaat aatgcattgt cagaacttag aaatcattca
     5761 attgatatgt tggcagatgt gaatcaaaaa cattttgatt taattaagtc ggataaaaat
     5821 ttaagcatta ttcgcaaaaa tggacgcaag tcagtctttt taatgctaaa tattaaaaaa
     5881 ggtatattta agacgcatcc aaacttgaga caagcagtag ttaatgcgat agatcaggat
     5941 caatttatta agttttatcg tggcgataaa tttaaaattg catcaccgat tacaccactt
     6001 gtcgatactg gtaacgagca acgtcaagat ttagaaaaag tagaaaaagc cattaatcaa
     6061 taatgtttta aatatttaac agaaagtagg aggatatagt atggtcatta acttaaatga
     6121 caaacagaca aaaacatcta aagaagggtt aatttccgta tcacatcctc ttgcggctaa
     6181 aattggtaag gatgtattag atcaaggtgg caacgccatg gatgcagtga ttgcaattca
     6241 actggcattg aatgtgttag aaccatttgc atcaggtatt ggtggtggcg ggtatttgct
     6301 atattatgag caaagtactg gcagtataac tgcgtttgat gcacgtgaga cagcacctga
     6361 acatgtagac aaacaatttt atctagatga ttcaggcgaa tataaatcat tttttgatat
     6421 gactacacat ggtaaaactg tcgctgtgcc agcaattcca aagctgtttg attatattca
     6481 caagcgttat gctaaattgt cattggaaga tttaattaat cctgcaattg aactagccat
     6541 tgaaggtcat gcagccaatt gggctactga aaaatattcg cgccagcaac acgcacgatt
     6601 gacaaagtat catgaaacgg cacaagtatt tacgcatgaa aatcaatatt ggcgtgaagg
     6661 tgattggatt gtacaacccg aattaggtaa gacatttcaa atattaagag aacaagggtt
     6721 taatgcattt tataaaggtg acattgcgaa acaattagtc aatgttgtca aagcatgtgg
     6781 tgggacaatc actttagagg atctagccaa atatgacatt cagattaaag cgccaatcag
     6841 tgcaacattt aaagactatg acatttattc aatgggacca tctagttctg gcggtatcac
     6901 ggtaattcaa atattgaagt tattagaaca tgtcgattta ccatctatgg gtccaagatc
     6961 tgttgattac ttgcatcatt tgatacaagc gatgcattta gcatatagtg atcgtgcgca
     7021 atacttggcg gatgataatt ttcatgaggt gcctgtacag tcattaattg atgatgatta
     7081 tttaaaagca cgcagtacgc tcattgatag caataaagca aatattgata tagagcatgg
     7141 tgttgtgtct gattgcatta gtcatacaga tgttgaagaa aatcataccg aaacaactca
     7201 tttttgtgtg attgataagg aaggtaatat tgcttcattt acgacatcaa ttggtatgat
     7261 ttatggttca ggtatcacga ttccaggcta cggtgtgtta ttgaatacga caatggatgg
     7321 ctttgatgta gtagatggtg gtattaacga aattgcacca tataaacgac cactaagtaa
     7381 catggctcca acgattgtga tgtatcacgg gaagccaata ttaacagtag gtgcacctgg
     7441 tgccataagt atcattgcta gtgttgcgca aacattaatc aatgtattag tgtttggcat
     7501 ggatattcag caggctatag atgaacctag aatttatagt agccatccta atcgcattga
     7561 atgggagcct caattttcac aatctacaat attagcattg attgcacatg gacatgcaat
     7621 ggaacataaa ccagatgctt atattggaga tgtacatgga ctacaggttg acccaacaac
     7681 gtatgaagct tcgggtggaa gtgatgatac tagagaaggc accgtaatgg gtggcgaggt
     7741 gttagtgatt agaaaacagc cattaccata tcggcaaatg tatgatagtg acggctttcg
     7801 tctatatttc aatgatgtgc agttaccttt attggcagat caagtgcgat ggatgcatga
     7861 caaatattgg gttgatgaaa gtgttgttag aatcattttc cctgaagtta gtgcacatat
     7921 tgaagattta agaagttatg aaaacgcagg agaaaattat atagatattg cctggttagc
     7981 acggaaatat gcttatcaag taacattgaa ggatgacggt ttatacttaa ctgatgatac
     8041 atatacttca gtgaaacgga acacaaatgc atactataga tatgatcgag atagtatcac
     8101 aagatagttt atacgcttga tatgaagttt gtagtggata tgagcttttg ttgaatgaaa
     8161 ggattcgatt tcgaacttta gcgtgacgct tgagtgtagt aatgttggaa atgctatacg
     8221 aaacgctagt cacctttgta aaatatacaa cgcctcacaa attcgttttt gatgtacatc
     8281 acaatagtta gcctaaaatc taatgtaatg tggtttggta cagttgaacg agttatgtga
     8341 ggtgttttta taatatagga ttttaataga tgatttggat gtttctataa ttacagcata
     8401 agtattaatg ttcacttatt ttattataag taaggctttc tttataaatg atttggtctt
     8461 ttccaggact tgaaaaataa aaataaagtt tctgatcttg atgattattg ccttttaaat
     8521 cactcattcc ctttaattcc acctttgttg atgcgttttt aggaatatta tatctacgtt
     8581 ttaattgttt aacgtttgga ttatcgttcg acaacttacc agataaatta taataatcag
     8641 tggtaggatc atgtgtgact ttcaaatcat tgctatttaa aatgcttgtt aaatcaccac
     8701 tttgaatcaa aaattgattg ttttcgattt tttgtttcag cgcgggatct tttacgtctt
     8761 ttgtgaaaac gattttatta ttaactactt ttactggata acttttgtat gtcgagtcag
     8821 tagcattttt ttatcgtttg tagttgtgtc atattcacca gttattttat gtgtgttctt
     8881 atctaccttt aacaacatac ggtcttcttt taaaagctca tctgatccaa caactgaata
     8941 agaggattct atataccatg tgtcttgatc attattttca taatggggat tatcgtgacc
     9001 atcaatttca taaagcgttt ctaagttttt aataggatac gtacttagta cttttttaag
     9061 accatctttc aaatgaattt gttcccactt cattgccaaa aacatatcgc cactgactac
     9121 aattgaaata ataataattg ctgctaagtt taaccagaaa attttatgtg ctttcataca
     9181 ttcccaccgt ttctcaaaat acttcattaa cactataata atatattttg aaaaatattt
     9241 acatcagtat taaagtgaat atcaaatttt aaatttatga aaataataga tatttataaa
     9301 aagcggaaaa gagatacaat aaaaaactgc atgacgtttg agacgtcaca cagtgtaact
     9361 aaaaatttaa aaagttgttg ctaatttttc agcattatta atactagttg ctttaatttc
     9421 ttcagtctta tgaggttcag cattgtgtcc ttcaataatg attgtttcat atgatggcac
     9481 acctaagaat gtcataattg ttcttaaata acggtcaccc atttcaaaat cagcagcagg
     9541 tccttcagta taatatccac cacgtgattg aatgtgtaat acttttttgt cagttagtaa
     9601 accttgtggt ccttcagcag aatatttaaa agttttacct gcaattgaaa tagcatcaat
     9661 atatgcttta actacaggtg ggaaagaaag gttccacata ggcgttacaa atacatattt
     9721 atctgcactt aaaaattctt ctaaaatgtc actcaatctt gaaactttca tttgttcatc
     9781 atcagttaac gtttcgccat tactcatttt tccccaacca gttaatacat ctttgtcaat
     9841 aactggaata taagtttcaa ataaatcaat atgtttcact tcatcatcag gatgttgttg
     9901 ttgatatgtt tcgataaatg ctttaccagc cgccatagaa tttgatacca gttcattaaa
     9961 agggtgtgct gtaatatata atacttttgc catttgaaaa ttctcctctg tttctgttat
    10021 tttcttaagt ataattatta tactcgatat aaaatttaat atcaatcaaa atattcaaat
    10081 taccatcatt ttcttcatct atatttggca gtactactaa agtatgagtg catttaatta
    10141 tgaaatagtt gatttagaat atatacttaa tacccaaaat atatgaagga tggatgccac
    10201 tatgacaaag cgaccaaaac gtattttggc aacaattatc atttttcttt cactattatt
    10261 tacgattatt tatatagatg acattcaaaa atggtttaac caatataccg ataaattgac
    10321 acaaaatcat aaaggacaag gacactcaaa atgggaagac ttttttagag ggagtcggat
    10381 tactgagact tttggtaaat atcaacattc accatttgat ggtaagcatt atggcattga
    10441 ttttgcattg ccaaaaggta caccaattaa agcgccgacg aatggtaaag taacacgtat
    10501 ctttaataat gaattgggcg gcaaggtatt acagattgcc gaagacaatg gagaatatca
    10561 ccagtggtat ctacacttag acaaatataa tgtcaaagta ggtgatcgag tcaaagcagg
    10621 tgatattatt gcatattcag gcaatacagg tatacaaacg acaggcgcac atttacattt
    10681 tcaaagaatg aagggtggcg taggtaatgc atatgcagaa gatccaaaac cgtttatcga
    10741 tcagttacct gatggggaac gtagcctata tgatttgtag ttatagaagg gtgcccgcag
    10801 tctaaaaaat taagcaatca ttgtgtgagt atgatactta cataatggtt gcttttttca
    10861 atgaaaatcg taatgctaag tcatacttgt ttgatttaga tattacttaa aatgtaagac
    10921 aaggttgtta gcattggcag tgaaatatcg cacataaaaa acattattgt cacactagaa
    10981 aatagttgtg cactatatca attttctgta taaaagttta attctgacag taatgtaaac
    11041 gtttataatt tatgattgac attaataatg actgaatata tgatttatgt aagtatttgt
    11101 gcaaacgttt tcacgaagtg tattgcacaa tcaaactgta aacaaagtat gggaggcata
    11161 acatggcaga actaaagtta gagcatatta aaaagacgta tgataacaac aatactgtag
    11221 tgaaagattt taatctacat attactgaca aagaattcat tgtatttgtt ggaccatcgg
    11281 gatgtggtaa atcaacaaca ttacgaatgg ttgctggact agagtctatc acatctggag
    11341 atttttatat tgatggggaa cgcatgaacg atgttgaacc aaagaataga gatattgcga
    11401 tggtatttca aaactatgca ttatatccac atatgactgt ttttgaaaat atggcatttg
    11461 ggctaaagct acgtaaagta aataaaaaag agattgaaca aaaagttaat gaagcagctg
    11521 aaatattagg attaactgag tatcttggtc gtaaaccaaa agcgttatct ggcggacagc
    11581 gtcaacgtgt tgctttgggc agagctattg ttagggatgc gaaagtcttt ttaatggatg
    11641 aaccattatc gaatcttgat gcgaagcttc gagtacaaat gcgcacagaa atattgaaat
    11701 tacataagcg acttaatact acgacaattt atgttacaca tgatcaaact gaagcattga
    11761 cgatggctag tcgaattgtt gttttgaaag atggcgacat tatgcaagtc ggcacaccta
    11821 gagaaatata tgatgcccct aattgcatat ttgtggcgca atttatcggc tcaccagcaa
    11881 tgaatatgtt gaatgctaca gttgaaatgg acggattgaa ggtaggaaca caccatttta
    11941 aattacataa taaaaaattt gaaaagttaa aagctgctgg ctacttagac aaggaaatta
    12001 ttttaggtat tcgagctgaa gacattcatg aagaaccaat atttattcaa acttctccag
    12061 agacacaatt tgaatctgaa gtagttgtat ccgaactgtt aggttcagaa attatggtac
    12121 atagcacatt ccaaggaatg gaattgattt ctaaattaga ttcaagaact caagtgatgg
    12181 cgaacgacaa gattacacta gcatttgata tgaataagtg tcactttttt gatgaaaaaa
    12241 caggaaatcg tatcgtctaa gggggagtat tcatgtctaa aattttaaaa tgtatcacgt
    12301 tagccgtggt aatgttatta atcgtaactg catgtggccc taatcgttcg aaagaagata
    12361 ttgataaagc attgaataaa gataattcta aagacaagcc taaccaactt acgatgtggg
    12421 tggatggcga caagcaaatg gcgttttata aaaaaattac ggatcaatat actaaaaaaa
    12481 ctggcatcaa agtaaagctt gtaaatattg gtcaaaatga tcaactagaa aatatttcgc
    12541 tagacgctcc tgcaggaaaa ggtccagata tctttttctt agcacatgat aatactggaa
    12601 gtgcctatct acaaggctta gctgctgaaa tcaaattatc aaaagatgag ttgaaaggtt
    12661 tcaataagca agcacttaaa gcgatgaatt atgacaataa gcaactagca ttgccagcta
    12721 tcgttgaaac aaccgcactt ttttataata aaaaattagt gaaaaatgca ccgcaaacgt
    12781 tagaagaagt tgaagctaat gctgccaaac taactgatag taaaaagaaa caatacggta
    12841 tgttatttga tgctaaaaat ttctatttta attatccgtt tttattcggc aatgatgatt
    12901 atattttcaa gaaaaatggc agtgaatatg atattcatca gctaggacta aattcaaaac
    12961 atgtcgtcaa gaatgctgaa cgattacaaa aatggtacga caaagggtat cttcctaagg
    13021 cagcaacaca tgatgtcatg attggtcttt ttaaagaagg aaaagtagga caatttgtca
    13081 ctggaccgtg gaacattaat gaatatcaag aaacgtttgg taaagattta ggagtaacaa
    13141 cattacctac agatggtggc aaacctatga aaccatttct aggtgtacgt ggttggtatt
    13201 tatctgaata tagtaaacat aagtattggg ctaaagattt aatgctgtat atcactagta
    13261 aagatacatt acaaaaatat acagatgaaa tgagcgaaat tactggacgt gttgacgtga
    13321 aatcatctaa tccaaattta aaagtgtttg aaaagcaagc acgtcatgct gaaccgatgc
    13381 ctaatattcc tgaaatgcga caagtttggg aaccgatggg caatgcaagc atatttattt
    13441 caaatggtaa gaatcctaaa caagcgttag atgaggcgac gaatgatata acgcaaaata
    13501 ttaagattct tcatccatca caaaatgata agaaaggaga ttagttatga cgaaacgtaa
    13561 ccctaaatta gcggcattat tatctgttat acctggtttg ggacagtttt ataataaaag
    13621 acccattaaa gggacgatat tttttatctt tttcatcagt tttatttctg ttttttatag
    13681 ctttttaaat attggttttt ggggattgtt cacattaggg acagtaccta agttagacga
    13741 ttctcgtgtc ttacttgcac aaggtattat ttctatctta ctcgttgctt tcgcaatcat
    13801 gctatatatc attaatattt tagatgcata tcgtaatgct gaacgattta atcgcaatga
    13861 ggaaataaag gatccgaagg cgcgtatggt ggcaacatgg gacaagacgt tcccatactt
    13921 actaatctca ccaggtacat tcttattgat atttgtagtt gtatttccat taatatttat
    13981 gtttggagta gcatttacaa attacaattt atacaacgcg cctccgagac acacattaga
    14041 atgggttggt ttagataact ttaaaacgtt attcacaatt ggcgtttggc gtaaaacatt
    14101 tttcagtgtt attacttgga cattagtatg gacgcttgtt gcaacgacac ttcaaattgc
    14161 attagggctg tttttggcaa ttattgtaaa tcaccctgtc gtcaaaggta agaaatttat
    14221 ccgtactgtg ttaatcctac cttgggctgt accatcattt gtgacaattt taatatttgt
    14281 agcgttattt aatgatgaat ttggtgcgat aaataatgat attttgcaac ctttattagg
    14341 tgtagcacca gcatggttaa gtgatccgtt ttgggcaaaa gtggcattaa tcggcattca
    14401 agtatggctt ggattcccat ttgtctttgc actgttcact ggagtactgc aaagtatttc
    14461 atcagattgg tacgaagcag cagatatgga tggtgcgtct agttggcaaa agtttagaaa
    14521 catcacattc ccgcatgtca tttacgccac agcgccattg ttaattatgc aatatgcagg
    14581 taatttcaat aattttaatc ttatttatct atttaataaa ggcggtccac cagtgtcagg
    14641 gcagaatgct ggtagtacag atatcttgat atcttgggtg tataatctga catttgagtt
    14701 taacaacttc aacatgggtg cagttgtgtc attaattatt ggatttattg ttgctattgt
    14761 cgcatttatt caattcagac gtacaagtac gtttaaagat gagggaggtt tataagatga
    14821 caaagaagaa aaacatatta aaagcaatcg gtatttacag ttttatagcg atgatgtttg
    14881 tcatcatttt atatccacta ctgtggacat ttggcatttc ccttaatcca ggtacgaact
    14941 tgtatggtgc caaaatgata ccagacaatg caacatttaa aaattatgca ttcttactat
    15001 tcgatgacag tagtcaatac ctgacttggt ataaaaatac gcttatcgta gcatctgcaa
    15061 atgcactgtt tagtgtgata tttgtcacgt taacagcata tgctttttct agatatcgct
    15121 ttgttggtcg taaatacggg ctgattacat ttttgatttt acaaatattc cctgtattaa
    15181 tggcaatggt cgcaatctat attttgctaa atacaattgg attattagat tctttatttg
    15241 gactaacact ggtatatatt ggtggatcaa taccgatgaa tgccttttta gtgaaaggtt
    15301 acttcgatac gattccaaaa gaacttgatg aatctgccaa aattgatggt gcagggcata
    15361 tgcgtatttt cttacaaatt atgcttccat tagctaagcc gattttagca gttgttgctt
    15421 tgttcaattt tatggggcca tttatggact ttatattacc taaaatacta ttaagaagtc
    15481 ctgaaaaatt cacattagca gttggattgt tcaactttat taatgataag tatgcaaata
    15541 atttcacagt gtttgcagca ggggcaatta tgattgcagt acctatagca atcgtattct
    15601 tgttcttgca acgctattta gtatcaggtt taacaacagg tgcgacaaaa ggttagtttg
    15661 aaattaggag tggggcagaa ttgataaaga accactaatg acgataaaga ttaaaaggag
    15721 gacgttatga tgacgattaa agttggaatc attgggtgtg gtggtattgc gaatggcaag
    15781 cacatgccaa gtttacaaaa agttgaaaat gttgaaatga tcgcattttg tgacgtagac
    15841 atttcgaaag cagcgagtgc ggcagaagca tacggaactg acaatgcaaa ggtttatgat
    15901 gattacaaag cattgttaaa agatgacacg attgatgtta tccatgtttg tacgccaaat
    15961 gactcgcatt gtgaaattac tgtagcaggg ttgcatgctg gtaaacatgt gatgtgtgaa
    16021 aaaccaatgg ctaaaacgac agcagaagct caaaaaatga tagatacagc taaatcaaca
    16081 ggtaaaaaat taacaatagg ttatcaaaat cgtttccgag cagatagtca atttttacat
    16141 caagcagcgc aacgtggcga cttaggagac atttacttcg gaaaggcaca tgccattcgt
    16201 cgtcgagcag taccaacatg gggtgtcttt ctagacgaag aagctcaagg tggaggacca
    16261 ttaatcgata tcggtacaca cgctttagat ttaacgttat ggatgatgga taattatgaa
    16321 ccagaatcag tgatgggttc aacattccat aaattaaata aacagcatca tgcggcaaac
    16381 gcttggggtt catggaatcc agatgaattt acagttgaag attctgcgtt tggatttatt
    16441 aaaatgaaga atggagcgac gatcatttta gaatccgctt gggcgattaa ttctttagaa
    16501 gtggatgagg caaaatgttc attatcagga actaaagcag gtgctgatat gaaagatggt
    16561 ctacgtattc atggtgaaga catgggtaca ctttatacca aacacgttga attggaaaac
    16621 aaaggcgtcg acttttatga aggtaatgaa gtggatgaag ctgaagaaga agcaaaagct
    16681 tggattgatg cagttgtaaa tgatactgaa ccagttgtga aaccggaaca agcaatggta
    16741 gttacaaaaa ttcttgaagc gatttatcag tctgcaaaat caggcaaagc aatttacttt
    16801 gaataacatc atacggtaag gaggcacatc atgacaaaat taaaagttgg tgtgataggt
    16861 gttggtggta ttgcacaaga ccgtcatatt ccagcattgc tgaaactcaa agacacagtc
    16921 tcattagttg cagtacaaga tattaataca gtgcagatga ttgatgttgc gaagcgcttt
    16981 aatatacctc atgcagttga gacacctagc gagctgttta aacttgttga tgcggtggtc
    17041 atttgtacac ctaataaatt ccatgctgat ctttctatag aagcattgaa ccatggtgtc
    17101 catgtattgt gtgaaaagcc aatggcgatg acgacggaag agtgtgatcg catgattgaa
    17161 gcggctaata aaaatcacaa attattaact gtcgcatatc attatcgtca cacagatgtg
    17221 gcaattactg ctaaaaaagc aattgaatca ggtgtggttg gtaaaccttt agtagcacgt
    17281 gtacaagcga tgcgtaggcg taaagtgcct ggctggggtg tttttaccaa taaagcgttg
    17341 caaggtggcg gtagtttaat cgattatggt tgccacttgt tagacttatc tttgtggcta
    17401 ctaggtaaag atatggtgcc gcatgaagtg ctaggaaaaa catataatca attgagcaaa
    17461 caaccgaatc aaattaatga ttggggaaca tttgatcata ctaaatttga tgtcgatgat
    17521 catgttacta gttatatgac atttgccaat cgagcaagca tgcagtttga atgttcgtgg
    17581 tctgcaaata tcaaagaaga taaggttcac gttagtttat caggagaaga tggcggtatc
    17641 aatttatttc catttgaaat atatgagccc cgctttggaa ctatttttga aagcaaagct
    17701 aatgttgagc ataacgaaga cattgctggt gagagacagg cgcgtaactt tgtcaatgcg
    17761 tgtttaggga tagaagagat tgtggtgaaa ccggaagaag cacgcaatgt aaatgccctt
    17821 atagaagcga tttatcgtag cgatcttgat aacaagagca tacaacttta atgattatca
    17881 tatatgatac aaaattctca atataaaaag aaggagtgct tttcaatgaa aataggtgta
    17941 ttttcagtat tattttacga taaaaatttt gaagatatgt tagattatgt ctcagaatct
    18001 ggattggata tgattgaagt tggaacaggt ggtaacccag gagataaatt ttgtaagtta
    18061 gatgagttgt tagaaaatga agacaagcgc caagcattta tgaagtcaat cacagacaga
    18121 ggcttacaaa taagtggttt cagttgtcat aacaatccaa tttctccaga tccgatagaa
    18181 gcgaaagaag ccgatgaaac gttacgtaaa acaatccgtt tagcaaatct attagacgtg
    18241 ccagttgtta atacattttc tggcattgca ggatcagatg ataccgctaa aaagcctaat
    18301 tggcctgtta caccttggcc aacagcctac tctgaaattt atgattatca gtggaatgaa
    18361 aagttgatac catattggca agatttagct gagtttgcaa aagagcaaga tgtaaaaatt
    18421 gccatagagt tgcatgcagg atttttagtg catacaccat atacaatgtt gaagttacgt
    18481 gaggctacaa atgaatatat cggtgctaac ttagatccta gtcatctatg gtggcaaggt
    18541 attgacccaa ttgctgcgat tcgcatatta ggccaagcaa atgcaattca tcacttccat
    18601 gctaaagata cgtatattaa tcaagaaaat gtaaatatgt atggtctaac tgatatgcaa
    18661 ccatatggta acgttgcgac aagagcatgg acattccgta cagttggtta tggacatagt
    18721 ccatatgtat gggcagatat cataagtcaa cttattatta atggatatga ttatgtatta
    18781 agtattgaac atgaagatcc tattatgtca gtagaagaag gtttccaaaa agcttgtcaa
    18841 actttgaaat ctgttaatat ttacgacaag ccagcagaca tgtggtgggc ataatacgaa
    18901 ctcgaggtta gtctgaagtt tgtctgaagt aagactggtg gcagtgttga ataaatgcat
    18961 atgtcgccaa gccattgcca aaaatttcac accttaaatc aagtcattgt ttgtaaagaa
    19021 ggtgtacttt atataagtat atagcgatgg tcatacccat tcacagtaac aatcctcacc
    19081 attgaaaaga gtatataacc ttttcaatag tgaggtatat gataataaaa aaaagcctgt
    19141 tgtcacaatg gtcatagaca cgacatactt taaaggtttc tgaatataat atttcagaat
    19201 gcactttaaa gatggacgtc gatgtagact aaagtgatga caggctttca tctttttaaa
    19261 tattcattaa tttctcttct tgtttaatac gtacatataa gaaatacgca tacggtacta
    19321 ataaaatagt tgtatatgtt gcgtgtgtta ataataatac accgattaat tcaggaatga
    19381 tgtttaagaa gtaatttggg tgttttgtaa ttttatataa tccagattta ataataggat
    19441 ggttaggtaa aatgaataat tttaatgtcc aaataccacc taaagtttta ataaccataa
    19501 ataacatgat ataagcaaag attaatataa ctaagccaat accatttgca aagctaaatg
    19561 tatctttatt aataaatgcc tctacaccag ccaatacata aattaaaacg tgtgttattg
    19621 ctaaaaactt cgaattttta acgccatatt caactgcacc gtctgctttt aattgttttg
    19681 agtgattaat agatatcttt aagctgacaa gtctgataca gaaaaagata agtaatatag
    19741 atagaatcat gatgtcctcc gtcattatgt catatgtata agcgttgatt ttgacaacat
    19801 aaagtatttt atagataaag cttgtcaaat actattaact atttattaat tttagtacat
    19861 aaatatgttt ctaagtatgt gtttatgttc agtattttgg ataatttaat aattttaagg
    19921 atattaagcg cttacaccga cgtgatatat ttggcttaac gaaaatgatt gaggtgacag
    19981 agatgaactt ttttgatatc cataagattc cgaacaaagg cattccatta tcggtacaac
    20041 gtaaattatg gcttagaaac ttcatgcaag ctttcttcgt agtgttcttt gtttatatgg
    20101 ctatgtattt aattcgaaac aactttaagg cggcacaacc gtttttaaaa gaggaaattg
    20161 gattatctac attagaactt ggttatatcg gattagcatt tagtatcacg tacggtttag
    20221 gaaaaacatt acttggatat tttgtcgatg gacgtaacac aaaacgtatt atctcgttct
    20281 tacttatctt atctgcgatt acagttttaa ttatgggatt tgttttaagt tactttggtt
    20341 ctgtaatggg attattaatt gtactttggg gacttaacgg ggtgttccaa tcagttggtg
    20401 gacctgcaag ttattcaacg atttcaagat gggcgccaag aacgaaacgt ggccgatact
    20461 taggattctg gaatacatca cataatatcg gtggtgccat agcaggtggt gttgcacttt
    20521 ggggtgctaa tgtattcttc catggaaatg ttatagggat gttcattttc ccatcggtga
    20581 ttgcattact tattggtatc gcaacattat ttatcggaaa agatgatccg gaagaattag
    20641 gatggaatcg tgctgaagaa atttgggaag agccggtcga taaagaaaat attgattctc
    20701 aaggtatgac gaaatgggag atctttaaaa aatatatcct gggaaatcct gttatatgga
    20761 ttctatgtgt ttcaaacgtc tttgtataca ttgtacgaat cggtattgat aactgggcac
    20821 cgttatatgt gtcagagcat ttacacttta gtaaaggcga tgcagttaat acgatattct
    20881 actttgaaat tggtgcatta gttgcaagtt tattatgggg ctacgtatca gacttattaa
    20941 aaggtcgtcg tgcaattgta gctattggct gtatgtttat gattacattt gttgtcttat
    21001 tctacacaaa tgctacaagt gtcatgatgg ttaacatttc attgtttgca ttaggtgcgt
    21061 taatctttgg tccgcaatta ttaattggtg tatcattgac tggttttgtt cctaaaaatg
    21121 ccatcagtgt agcaaacgga atgacaggtt cattcgcgta tctattcggt gactcaatgg
    21181 cgaaagttgg tttggcggct attgctgatc caacacgtaa cggtttaaac atctttggat
    21241 atacattaag tggatggaca gatgttttca tcgtcttcta tgttgcatta ttcctaggca
    21301 tgattctatt aggaatcgtt gctttctatg aagaaaagaa aattagaagt ttaaaaattt
    21361 aatataaatc ggattaaaag tatcgccaat ctattgcaat atagttggca atcctgcccc
    21421 gacggcatgt gcgtgaagag atgaaagata ctgcttctac ccttgcaaat atatcatctc
    21481 tatgtctcgg ggcagatcat aattccctgt tatgaagtat ccttatttgc ccgacttagg
    21541 gtgactcaat gaatttactc cttacaataa agacatatag cggtgtcaat attgtaggga
    21601 gtattgtttt atatttaaac tctctaaaaa gcggactgaa agaaaagtga aaacttctct
    21661 atcagtccgc tttttcatag aacaaaatgg aggcgccata atcattagtt atgtgctaat
    21721 ctattttgct tgcttacaat aatcacttgg cgacatttgt aaatattttt taaaatgata
    21781 gctaaacatt ttatactctg aaaagcctac tttgtctgca atttcatagt gtttgtaatg
    21841 tcgatctaac aattgcagag attgtaaaat acgatagcga tttaaataat cgacaattgt
    21901 aataccaaca tgatctttaa atgttcgcat cgcatacgat tcactaacat cgatatgttg
    21961 aattaaatct gaaacagtca ctttcgtttg ataagattgc ttaatttgat ccacaatctg
    22021 gtttacataa taatcatcgt attctacttt taatagtggt tggaaggcat catgacaaga
    22081 tgctaagcta cggccgttct gtgattgttg ctctaataag gtacggacaa gtcttcctaa
    22141 aataacttct aattgtgcat ggtctactgg ttttaataaa taatcaagaa catgatgttg
    22201 aatgccggct ttcatatatt caaagtcatc gtaactcgat aatatgatga cattacaatc
    22261 tagatgcgca atatcattga gtaaatcgac gccattttta cgtggcatac gaatatcagt
    22321 aattactaat tctggctgat gttgttgaat tagtgataat gcttcaacac catctttagc
    22381 agtgtatatt gtattgaaat gatagtctcc ccaaggaatg atttgcttta atccttctcg
    22441 aataattcgt tcatcatcac aaataactac cttaaacatc tacattcccc cttgaaagtg
    22501 gtattttata acaaattaac gtaccttgat tacgctttga aaaaatatgg agtcgtgcat
    22561 gtgaaccata ttgaatcatt gctttattgt gtaaatgatt taatcccaaa tgcttagtat
    22621 caaatacatc attattaaga gattggcgta catattgcag gcgagatgac gacatcccga
    22681 taccattgtc gcaaactaaa acatgtaaat tctgacgtgc caatgtcagg cgtatagtaa
    22741 tgtccaatga ctcagtatct ctaccatgtt taatagcatt ttctatgagt ggctgaagca
    22801 tcattttacc aattgtctgg tgacgcgctt cttcagaact ttcaatatgg agcttaatca
    22861 tgtcatcaaa acggatgttt tgtattgcaa catactgttc aatgtagttc aactcttcgt
    22921 ttaattccac tgtatgtgag tttgtacgta atgagtaacg taacatttgc gataattgtt
    22981 ggaccacagt ttgtgctaat ttcggagata acgtaattaa atattgtatt gtttgcatcg
    23041 tattgaatag gaaatgaggc tggaattggc gttctatttc ctttaactga atatcacgca
    23101 agcgacgttc tgtatgctcg atagaatgga tcagttgctc atttgattca aataaatcgt
    23161 aaatataatt attaatttct tctagttcac tgttgttttt taaaggcgta tatgtaccta
    23221 gatgacgatt tttggcatag taaatttttt gaataatcgt ttcgatatct tttgtttgtc
    23281 gtttagccat attatctgcg ctaatgaaac caaatattac tagtaaaaca agaactacgg
    23341 ccataacaat taacaacgtg ataccatctt caatgttttc atgtatatct ttataaataa
    23401 tgagacgatg gtcagcatgg tttaatttta cagattcatt cataaatccg aattgttgtg
    23461 gtctatactt ttcacctata gtaaaacggt catcgttggc gtataaaata ttgtcatatt
    23521 gatcaacgat aagtgcgaat tgtcggttat ctttcttaat ttcacttaaa cgtggggtgt
    23581 tagccatata aattttaagc atatatgtac tatttttgaa tttaagctga tgcgttgaaa
    23641 ataaatacat atttttagtg tttaaatgtt cataattatt ggttataaac tgatttggtc
    23701 cagataattc ataataaagt gttgcgggct gttggtgtat taattttaat aattcacgtt
    23761 ttgtagcggt cacatcatga tgatttgtta aatcgagctc ttgaaacgaa ttattatgct
    23821 gtgtaataaa tgtctgaatc tgcttttcag tatgatgtaa agatgactga ctttcatcaa
    23881 catgttgatg aatcgtacga tgctcaatcc aaatatagat ggcatagaag cttactagtc
    23941 caataataat gactaaaaat actggaaaaa tagtagacgc aaataacgat cgtcttaatt
    24001 gatgtctata aggtttgtat gccgtcattg aatcatctcc aaaaatttat gatgtggaat
    24061 atccggtaat ttagatttcg gtattaaagg tatgttctta agattttcga tagactgatc
    24121 gctttgttca ctaacatcct ttcgaattga cttggcatcg aactctgcaa ctaatcgttg
    24181 ttgtactgag cggcttgtta aatattgcac taacttttta cgcttaggat gagggtgtgc
    24241 atttttaact aaagcaatac catcaacatt taacattgtt ccttcaattg gataaacgat
    24301 tgatacagga taacctttgt ttttccatgt gcgtgcatct tgttcgtagc ttagacctgc
    24361 gtaatattta ccttttgcaa catcttcaat gactttagac gtctttgaca gttgcatcgc
    24421 atggttttgg aattgatgca catcacttac tcgatgatgc atgctataaa tagcacgcat
    24481 atgttgatag cctgtcgttg ttgtatttgg atttgagtac gcaattttac ctttaagtat
    24541 aggttgtaat aaatcttgat aacctcgaat cttaatatct ccttgtaaat ctgaattcac
    24601 tactataact gttggcatta atagaaaact agtaacatat ttattgttcg agcgataatc
    24661 ctctaattgc tgtgttacag atgtatcttg atagggaaca aaatcttctg gatgatcaat
    24721 tgtttctgac aacacaccac ccataaagac atcaccacgc tccgaaaaat cttcgttatg
    24781 caagtttgaa agcagtactt gagtagatcc gtgtttaatt tcaattttga catgctcttg
    24841 tttttcaaat tcatttaaaa ttggacgaat caagtttgat tgatacggag aataaactgt
    24901 taatacattt ttatcggatt cagagtgacg cgtattagcg catgctgata aaaaaatgag
    24961 aaataatagc aagatataaa tttttgattt catgatatcc catcaattct atgtatattt
    25021 taatacaata attttagcaa taaatgacgc ataagtaatg ttaaatattt agaaatgttt
    25081 atagatgact tgttaagacg ttgcaaatgt tgtgatagca caaaattttt gtttgtcaag
    25141 acgatttacc gaggctgtaa aatcaaactg ttatatttta tttgtagctg ttatataaaa
    25201 atcggcaaga tattgaacgg ttcaaaagtg aatttttacg tcaataaaag tatttaatcc
    25261 agtctcttca tatataaaag taaatctttc taagtgttga tttaacgctt atcaacaatc
    25321 attttttata aacaaatata tactcctaaa ttaactttta aagcaatgaa aatagtgaac
    25381 attataactg ttgtgtaaca gaatgcaatt agcatattac tgttacacaa attagtacag
    25441 tttctatgtt ttgacataca tttgatgaaa attgtacata atttatgtga aaaaaatcac
    25501 aacaaacatg ctacaatgac tatgaaaacg ttaacatagc atttcaaatt cacaacatta
    25561 tacagatgga ggcgtttagt atgttagaaa caaataaaaa tcatgcaaca gcttggcaag
    25621 gatttaaaaa tggaagatgg aacagacacg tagatgtaag agagtttatc caattaaact
    25681 acactcttta tgaaggtaat gattcatttt tagcaggacc aacagaagca acttctaaac
    25741 tttgggaaca agtaatgcag ttatcgaaag aagaacgtga acgtggcggc atgtgggata
    25801 tggacacgaa agtagcttca acaatcacat ctcatgatgc tggttattta gacaaagatt
    25861 tagaaacaat tgtaggtgta caaactgaaa agccattcaa acgttcaatg caaccattcg
    25921 gtggtattcg tatggcgaaa gcagcttgtg aagcttacgg ttacgaatta gacgaagaaa
    25981 ctgaaaaaat ctttacagat tatcgtaaaa cacataacca aggtgtattc gatgcatatt
    26041 ctagagaaat gttgaactgc cgtaaagcag gtgtaatcac tggtttacct gatgcatacg
    26101 gacgtggacg tattatcggt gactatcgtc gtgtagcttt atatggtgta gatttcttaa
    26161 tggaagaaaa aatgcacgac ttcaacacga tgtctacaga aatgtcagaa gatgtaattc
    26221 gtttacgtga agaattatca gaacaatatc gtgcattaaa agaattaaaa gaacttggac
    26281 aaaaatatgg tttcgattta agccgtccag cagaaaactt caaagaagca gttcaatggt
    26341 tatacttagc ataccttgct gcaattaaag aacaaaacgg tgcagcaatg agtttaggtc
    26401 gtacatcaac attcttagat atctatgctg aacgtgacct taaagcaggc gttattactg
    26461 aaagcgaagt tcaagaaatt attgaccact tcatcatgaa attacgtatt gttaaatttg
    26521 ctcgtacacc tgattacaat gaattattct ctggagaccc aacttgggta actgaatcta
    26581 tcggtggtgt aggtattgac ggacgtccac ttgttacgaa aaactcattc cgtttcttac
    26641 actcattaga taacttaggt ccagctccag aaccaaactt aacagtatta tggtcagtac
    26701 gtttacctga caacttcaaa acatactgtg caaaaatgag tattaaaaca agttctatcc
    26761 aatatgaaaa tgatgacatt atgcgtgaaa gctatggcga tgactatggt atcgcatgtt
    26821 gtgtatcagc gatgacaatt ggtaaacaaa tgcaattctt cggtgcacgt gcgaacttag
    26881 ctaaaacatt actttacgct atcaatggtg gtaaagatga aaaatctggt gcacaagttg
    26941 gtccaaactt cgaaggtatt aacagcgaag tattagaata tgacgaagta ttcaagaaat
    27001 ttgatcaaat gatggattgg ctagcaggtg tttacattaa ctcattaaat gttattcact
    27061 acatgcacga taaatacagc tatgaacgta ttgaaatggc attacatgat acagaaattg
    27121 tacgtacaat ggcaacaggt atcgctggtt tatcagtagc agctgactca ttatctgcaa
    27181 ttaaatatgc acaagttaaa ccaattcgta acgaagaagg tcttgtagta gactttgaaa
    27241 tcgaaggcga cttccctaaa tacggtaaca atgacgaccg tgtagatgat attgcagttg
    27301 atttagtaga acgcttcatg actaaattac gtagtcataa aacatatcgt gattcagaac
    27361 atacaatgag tgtattaaca attacttcaa acgttgtata cggtaagaaa actggtaaca
    27421 caccagacgg acgtaaagct ggcgaaccat ttgctccagg tgcaaaccca atgcatggcc
    27481 gtgaccaaaa aggtgcatta tcttcattaa gttctgtagc taagatccct tacgattgct
    27541 gtaaagatgg tatttcaaat acattcagta tcgtaccaaa atcattaggt aaagaaccag
    27601 aagatcaaaa ccgtaactta actagtatgt tagatggtta cgcaatgcaa tgtggtcacc
    27661 acttaaatat taacgtattt aaccgtgaaa cattaataga tgcaatggaa catccagaag
    27721 aatatccaca gttaacaatc cgtgtatctg gttacgctgt taacttcatt aaattaacac
    27781 gtgaacaaca attagatgta atttctcgta cattccatga aagtatgtaa caaaatttaa
    27841 ggtgggagca ctatgcttaa gggacactta cattctgtcg aaagtttagg tactgtcgat
    27901 ggaccgggat taagatatat attatttaca caaggatgct tacttagatg cttgtattgc
    27961 cacaatccag atacttggaa aattagtgag ccatcaagag aagtcacagt tgatgaaatg
    28021 gtgaatgaaa tattaccata caaaccatac tttgatgcat cgggtggcgg tgtaacagtc
    28081 agtggtggcg aaccattgtt acaaatgcca ttcttagaaa aattatttgc agaattaaaa
    28141 gaaaatggtg tgcacacttg cttagacaca tcggctggat gtgctaatga tacaaaagca
    28201 tttcaaaggc attttgaaga attacaaaaa catacagact tgatattatt agatataaaa
    28261 catattgata atgacaaaca tattagattg acaggaaagc ctaatacaca catccttaac
    28321 ttcgcgcgca aactgtcaga tatgaaacaa cctgtatgga ttcgacatgt ccttgtgcct
    28381 ggttattctg atgataaaga cgatttaatt aaactagggg aatttattaa ttctcttgat
    28441 aacgtcgaaa agtttgaaat tctgccatat catcagttag gtgttcataa gtggaaaaca
    28501 ttgggcattg catatgaatt agaagatgtc gaagcgcccg atgatgaagc tgttaaagca
    28561 gcctaccgtt atgttaactt caaagggaaa attcccgttg aattataaat acaattcaga
    28621 ccgaaaagaa agcatatgca acttcaagag tgaaggggca tatgcttctt tttcaattga
    28681 gtattgagta ttagcaagac gtagtaagta tatgagacaa cttctacaat ggttgaagga
    28741 agacgttttt gtaagtagct atgctgataa agaatgtgat gtcttgttaa aggtggggtt
    28801 ccaatatcat catttagctg atgttgaatg ggttattatt tgctacttgc atatgaatat
    28861 gagtcttttc aaatttttat tgaccctgag taatgaaaaa tattaagatg aaacttaata
    28921 ttaaagcaat gcggagcgtg attatgaaga gaattagtaa agatatatgg gcagtattta
    28981 aattactgta tcaaaataaa gggcgtttta gcattaatgc cttactattg cagttaatca
    29041 tgatttttat tagtagtaca tacttaattt tactatttaa tatgatgtta aaagtagctg
    29101 ggcaaagcca acttacgatt aacaattgga cggaaatcgt tagtcatccc gccagtgtga
    29161 tacttcttat tatattcata ttaagtgttg cctttctgat ttatgtagag ttttcattgt
    29221 tagtttatat ggtttatgcc ggctttgatc gacagattat tacatttaaa tccattttta
    29281 aaaatgcctt tgtaaatgtg cgtaaactca taggtgtacc agttattttc tttgtcattt
    29341 atttaatgtt aatgataccc attgccaacc taggactaag ttcagtatta acaaaaaata
    29401 tttacatacc taaattttta acggaagaac ttatgaaaac gacgaaaggt ataatcattt
    29461 acggtacctt tatgattgct gtatttatat taaattttaa attaatattt actctaccgt
    29521 taacgatttt aaaccgccag tcgttattta aaaatatgag actaagttgg caaattacga
    29581 agcgaaataa gtttcggctt gttatagaaa tagttatatt agaactcatc attggtgcga
    29641 ttttaacatt aattatttca ggagcaacat atcttgctat ttgtgtagat gaagaaggag
    29701 ataagttttt agtctcatca attttatttg ttgtattgaa aagcgcattg ttcttctatt
    29761 atttatttac gaaattatca ttaatcagtg tgttagtact gcacttaaaa caagagaatg
    29821 tattagacca accgggctta gaatttaaat atccaaaacc gaaacggaag tctaggttct
    29881 ttataatttc aatggtgctt gcagtgacat gttttatcgg ttataacatg tacttacttt
    29941 acaataatac tatcaataca aatatctcca ttattggcca tcgtggtttc gaagataaag
    30001 gcgttgaaaa ttctattccg tcattgaaag ctgctgcaaa agcgaatgtc gaatacgttg
    30061 agttagatac aattatgacg aaagataaac aatttgttgt tagtcatgat aacaatttga
    30121 aacgtttaac aggtgttaat aaaaatattt ctgaatctaa tttcaaagat atcgtcggtt
    30181 tgaaaatgcg tcaaaatgga catgaagcaa aatttgtatc cttagacgaa tttattgaaa
    30241 cggctaaaca atcaaatgtg aagctactag tagagttaaa gccacatggt aaagaaccag
    30301 cagattatac acaacgtgtt attgatattt tgaaaaagca tggtgttgaa catcaatatc
    30361 gtgtgatgtc tttggattat gatgtgatga ctaagttgaa aaaagaagcg ccatatctca
    30421 agtgtggtta tatcattccg ttgcagtttg gtcattttaa agaaacatca ttagatttct
    30481 ttgtcatcga agatttttct tattcgccaa gacttgttaa tcaagcgcac ttggaaaata
    30541 aagaagtcta tacttggact attaacggcg aagaagattt aacgaaatac ttacaaacca
    30601 atgttgatgg tattatcaca gatgacccag cattagctga tcagattaaa gaagaaaaga
    30661 aagacgaaac atacttcgat cgttctataa gaattttgtt tgaataatat aaacaaagac
    30721 ctctaaagtt atcaagatga taccttcaga ggtcttttta atgttgccat ctatgggata
    30781 ggcaatcgtt tcattcgttt atattcatat gacaagtatt tgtatggcaa tttggcgtca
    30841 caaacactta catgatttat tggtgaatta ttaattgttt tgtgaatgca aagggttaga
    30901 aattgaattg taaatacttt ctaatctttg tttcgcttta gtcatttgat ccaaattttt
    30961 agtgcgtata gcggattttg caatatagtg cgcagctaaa atatcgcgtt tttgaaacgc
    31021 atctaaattt aggtacgata atttatttaa gtcagtgttt gctattaatt catgtaattg
    31081 atctacaagc gcttgatgtt gatacgtatg tgatgtagtt tcagatttgc ttgctaattt
    31141 aataccagtc gtatcaagga gcgccgcttt aataccagca actaaatatg ttttgatttt
    31201 catttgtgtt gtcatgcttt gttactcctt tgatgtacat taatcaaaaa aattatacac
    31261 tattgtatat tgcaaagcta attaactata acaaaaagat agttaatgct ttgtttattc
    31321 tagttaatat atagttaatg tcttttaata ttttgtttct ttaatgtaga ttgggcaatt
    31381 acattttgga ggaattaaaa aattatgaaa aagcaaataa tttcgctagg cgcattagca
    31441 gttgcatcta gcttatttac atgggataac aaagcagatg cgatagtaac aaaggattat
    31501 agtgggaaat cacaagttaa tgctgggagt aaaaatggga cattaataga tagcagatat
    31561 ttaaattcag ctctatatta tttggaagac tatataattt atgctatagg attaactaat
    31621 aaatatgaat atggagataa tatttataaa gaagctaaag ataggttgtt ggaaaaggta
    31681 ttaagggaag atcaatatct tttggagaga aagaaatctc aatatgaaga ttataaacaa
    31741 tggtatgcaa attataaaaa agaaaatcct cgtacagatt taaaaatggc taattttcat
    31801 aaatataatt tagaagaact ttcgatgaaa gaatacaatg aactacagga tgcattaaag
    31861 agagcactgg atgattttca cagagaagtt aaagatatta aggataagaa ttcagacttg
    31921 aaaactttta atgcagcaga agaagataaa gcaactaagg aagtatacga tctcgtatct
    31981 gaaattgata cattagttgt atcatattat ggtgataagg attatgggga gcacgcgaaa
    32041 gagttacgag caaaactgga cttaatcctt ggagatacag acaatccaca taaaattaca
    32101 aatgaacgta ttaaaaaaga aatgattgat gacttaaatt caattattga tgatttcttt
    32161 atggaaacta aacaaaatag accgaaatct ataacgaaat ataatcctac aacacataac
    32221 tataaaacaa atagtgataa taaacctaat tttgataaat tagttgaaga aacgaaaaaa
    32281 gcagttaaag aagcagatga ttcttggaaa aagaaaactg tcaaaaaata cggagaaact
    32341 gaaacaaaat cgccagtagt aaaagaagag aagaaagttg aagaacctca agcacctaaa
    32401 gttgataacc aacaagaggt taaaactacg gctggtaaag ctgaagaaac aacacaacca
    32461 gttgcacaac cattagttaa aattccacag ggcacaatta caggtgaaat tgtaaaaggt
    32521 ccggaatatc caacgatgga aaataaaacg gtacaaggtg aaatcgttca aggtcccgat
    32581 tttctaacaa tggaacaaag cggcccatca ttaagcaata attatacaaa cccaccgtta
    32641 acgaacccta ttttagaagg tcttgaaggt agctcatcta aacttgaaat aaaaccacaa
    32701 ggtactgaat caacgttaaa aggtactcaa ggagaatcaa gtgatattga agttaaacct
    32761 caagcaactg aaacaacaga agcttctcaa tatggtccga gaccgcaatt taacaaaaca
    32821 cctaaatatg ttaaatatag agatgctggt acaggtatcc gtgaatacaa cgatggaaca
    32881 tttggatatg aagcgagacc aagattcaat aagccatcag aaacaaatgc atataacgta
    32941 acaacacatg caaatggtca agtatcatac ggagctcgtc cgacacaaaa caagccaagc
    33001 aaaacaaacg catataacgt aacaacacat ggaaacggcc aagtatcata tggcgctcgc
    33061 ccaacacaaa acaagccaag caaaacaaat gcatacaacg taacaacaca tgcaaacggt
    33121 caagtgtcat acggagctcg cccgacatac aagaagccaa gtaaaacaaa tgcatacaat
    33181 gtaacaacac atgcagatgg tactgcgaca tatgggccta gagtaacaaa ataagtttgt
    33241 aactctatcc aaagacatac agtcaataca aaacattacg tatctttaca acagtaatca
    33301 tgcattctat gatgcttcta actgaattaa agcatcgaac aatcggaagc atatttctaa
    33361 attatttatt cattatagtc t
//
LOCUS       DANRDI010000013        32876 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.013, whole genome shotgun sequence.
ACCESSION   DANRDI010000013 DANRDI010000000
VERSION     DANRDI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32876
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.013"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..556
                     /locus_tag="PZ377_000442"
     CDS             <1..556
                     /locus_tag="PZ377_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357333.1"
                     /translation="IVVLITGFNLYRHQMQHLSLSAMLLSITINAFINTFIIDKYNFI
                     TLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVTDFSSTQSIYM
                     SIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINISFIVMMVLTP
                     YWYLYLIVYLIFLLFLLWQKVYKF"
     gene            836..1276
                     /locus_tag="PZ377_000443"
     CDS             836..1276
                     /locus_tag="PZ377_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDM6357334.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            1618..2070
                     /locus_tag="PZ377_000444"
     CDS             1618..2070
                     /locus_tag="PZ377_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDM6357335.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKNR"
     gene            2067..2753
                     /locus_tag="PZ377_000445"
     CDS             2067..2753
                     /locus_tag="PZ377_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6357336.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2753..3808
                     /gene="saeS"
                     /locus_tag="PZ377_000446"
     CDS             2753..3808
                     /gene="saeS"
                     /locus_tag="PZ377_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDM6357337.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3880..4863)
                     /gene="csbB"
                     /locus_tag="PZ377_000447"
     CDS             complement(3880..4863)
                     /gene="csbB"
                     /locus_tag="PZ377_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDM6357338.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(4995..5834)
                     /locus_tag="PZ377_000448"
     CDS             complement(4995..5834)
                     /locus_tag="PZ377_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDM6357339.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(6056..7405)
                     /locus_tag="PZ377_000449"
     CDS             complement(6056..7405)
                     /locus_tag="PZ377_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDM6357340.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(7626..8807)
                     /gene="nagA"
                     /locus_tag="PZ377_000450"
     CDS             complement(7626..8807)
                     /gene="nagA"
                     /locus_tag="PZ377_000450"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDM6357341.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(9115..11073)
                     /locus_tag="PZ377_000451"
     CDS             complement(9115..11073)
                     /locus_tag="PZ377_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDM6357342.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(11079..11999)
                     /gene="pfkB"
                     /locus_tag="PZ377_000452"
     CDS             complement(11079..11999)
                     /gene="pfkB"
                     /locus_tag="PZ377_000452"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDM6357343.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11996..12760)
                     /locus_tag="PZ377_000453"
     CDS             complement(11996..12760)
                     /locus_tag="PZ377_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDM6357344.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(13010..13492)
                     /gene="ybaK"
                     /locus_tag="PZ377_000454"
     CDS             complement(13010..13492)
                     /gene="ybaK"
                     /locus_tag="PZ377_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDM6357345.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(13666..14118)
                     /locus_tag="PZ377_000455"
     CDS             complement(13666..14118)
                     /locus_tag="PZ377_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357346.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(14411..15577)
                     /gene="norA"
                     /locus_tag="PZ377_000456"
     CDS             complement(14411..15577)
                     /gene="norA"
                     /locus_tag="PZ377_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDM6357347.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            15786..16208
                     /locus_tag="PZ377_000457"
     CDS             15786..16208
                     /locus_tag="PZ377_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDM6357348.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            16394..17011
                     /locus_tag="PZ377_000458"
     CDS             16394..17011
                     /locus_tag="PZ377_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDM6357349.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            17008..17292
                     /locus_tag="PZ377_000459"
     CDS             17008..17292
                     /locus_tag="PZ377_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357350.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(17447..18820)
                     /locus_tag="PZ377_000460"
     CDS             complement(17447..18820)
                     /locus_tag="PZ377_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDM6357351.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(18906..20459)
                     /locus_tag="PZ377_000461"
     CDS             complement(18906..20459)
                     /locus_tag="PZ377_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDM6357352.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(20742..21029)
                     /locus_tag="PZ377_000462"
     CDS             complement(20742..21029)
                     /locus_tag="PZ377_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357353.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVEHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(21064..21972)
                     /locus_tag="PZ377_000463"
     CDS             complement(21064..21972)
                     /locus_tag="PZ377_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDM6357354.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            complement(22075..23001)
                     /locus_tag="PZ377_000464"
     CDS             complement(22075..23001)
                     /locus_tag="PZ377_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDM6357355.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            23228..23671
                     /gene="mgrA"
                     /locus_tag="PZ377_000465"
     CDS             23228..23671
                     /gene="mgrA"
                     /locus_tag="PZ377_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDM6357356.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            complement(23798..25471)
                     /locus_tag="PZ377_000466"
     CDS             complement(23798..25471)
                     /locus_tag="PZ377_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDM6357357.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(25468..27099)
                     /locus_tag="PZ377_000467"
     CDS             complement(25468..27099)
                     /locus_tag="PZ377_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDM6357358.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            27311..28186
                     /locus_tag="PZ377_000468"
     CDS             27311..28186
                     /locus_tag="PZ377_000468"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDM6357359.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            28358..29041
                     /locus_tag="PZ377_000469"
     CDS             28358..29041
                     /locus_tag="PZ377_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357360.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            29044..29502
                     /locus_tag="PZ377_000470"
     CDS             29044..29502
                     /locus_tag="PZ377_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDM6357361.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            29504..30070
                     /locus_tag="PZ377_000471"
     CDS             29504..30070
                     /locus_tag="PZ377_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDM6357362.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(30166..30708)
                     /locus_tag="PZ377_000472"
     CDS             complement(30166..30708)
                     /locus_tag="PZ377_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357363.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(30788..31087)
                     /locus_tag="PZ377_000473"
     CDS             complement(30788..31087)
                     /locus_tag="PZ377_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357364.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            complement(31225..31620)
                     /locus_tag="PZ377_000474"
     CDS             complement(31225..31620)
                     /locus_tag="PZ377_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357365.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            complement(31687..32130)
                     /locus_tag="PZ377_000475"
     CDS             complement(31687..32130)
                     /locus_tag="PZ377_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357366.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            32257..>32876
                     /locus_tag="PZ377_000476"
     CDS             32257..>32876
                     /locus_tag="PZ377_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDM6357367.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFS"
BASE COUNT        11031 a         5848 c         4624 g        11373 t
ORIGIN      
        1 tatcgtcgta cttataactg gattcaattt atataggcat caaatgcaac atttatcatt
       61 aagtgcaatg ttgttatcta ttacgataaa cgcctttata aatacattca taattgataa
      121 atataacttt attactctgt gtactataac gatgtttatc atttggacga tactacagtt
      181 ttacattgat aaaaaattaa aacctgtata cattacagac caaaaattta ttgctatcat
      241 attaacgatt gttgtcagtt taacgcaacg tgttaccgac ttttcaagta cacaatcaat
      301 atatatgtct attccatttt tagcccctgc cattttcatc attggtggta ttatgttgtt
      361 tatttcaaca tttaatagct tagatgaaac tgctgaaaat aataacaaaa taaagaaact
      421 aatgattaaa ggacttatca ttattaacat ttcatttatc gttatgatgg ttttaacacc
      481 atattggtac ttgtatttaa tcgtctatct tattttcttg ttgtttttat tgtggcaaaa
      541 ggtttataaa ttttaatacc aaaactatta aacacttctg atattcttag ttcaaaatat
      601 cagaagtgtt ttatagtgtt atctagttca gataaatatt tccttactta aaaaaacgcc
      661 ctcctcttat tttgacccct atttatttaa atcagacaat tattttcatt ttcaaattat
      721 tctttcttca atattagtta agcgatattt aaacgaagtt aagaattagt taatggcata
      781 ttatttgcct tcattttaaa cttaacttat caaattgaag aaatgaggag ttagcatgaa
      841 tacaaaatat tttttagcag ctggtgctgt tatcacaaca ttagctttag gtgcttgtgg
      901 taattctaat tcacaagatc aaggtaacaa aactgaacaa aaaacaaagt cagaagatag
      961 caatgttaaa actgataaaa caaaacacct aacaggtaca ttcagttcta aaaacggtga
     1021 aactgttgaa ggtaaagctg agattaaaaa tggtaaatta atgcttacta actacaaatc
     1081 atcaaaaggt ccagatttat acgtctacct aacaaaaaat ggcgacatta aaaacggtaa
     1141 agaaatcgca atggttgact acgataaaga aaaacaaaca tttgatctta aaaatgtaga
     1201 tttaagcaaa tatgatgaag taacaatcta ttgtaaaaaa gctcacgtca tcttcggcgg
     1261 cgctaaatta aaataatcaa tttctgagtt aaacttttat ttacaacata ctattactat
     1321 acattacaaa tttttaaaat atacattaca ctcattactc aatggaaagc gtatgatttc
     1381 acagcccccc tagcttgtag aaatcatact ttcctttttt caatatatat acaactatta
     1441 aatcccataa gattgcagag cacataagta aattttttta gagcttgagg tttgtttagc
     1501 ttaagcaacc catgagctca aacacttcct gttcacataa cactacaaat cgcattatgt
     1561 tgcttaatct tatgtttata taaattacac acaataaata gaaagaatgt gaacatcatg
     1621 aataaattat tgctactcgt tacatttatc attcgtgtgg gttcaggtat tgttatgtta
     1681 atgcaaggct acgaaaaatt aacgggcgga tttacgctga aaggtttagt accagtcatc
     1741 gctaacaata ctgattcacc agagtggtat aagtggtttt tcgcaaatat agttgcacat
     1801 acgacgtcat tatttgatat tgttgtccca ctcggagaga ttgcaattgg attaggttta
     1861 atttttggag tttttgcata tgctgctagt ttctttggag cctttgttat gataaattat
     1921 atcttagcag atatgatatt tacgtatcct cttcaattaa ctttctttat ccttttacta
     1981 atgagtcact cattgttaaa acagatttca cttaaagaaa tcattaatta ctttagaggt
     2041 cgtaagaaca gaggtgaaaa aaatagatga cccacttact gatcgtggat gatgaacaag
     2101 acattgtaga catttgtcaa acctattttg aatatgaagg ttacaaagta acaacgacaa
     2161 ctagcggtaa agaagcaatt tctttactat caaatgatat tgatatcatg gtacttgata
     2221 tcatgatgcc agaagttaat ggttacgaca ttgtcaaaga aatgaaaagg caaaaattag
     2281 atatcccctt tatctattta actgccaaaa cacaagaaca tgataccatt tacgccttaa
     2341 ctttaggtgc agatgactat gtcaaaaaac catttagtcc aagggaactc gttttacgta
     2401 ttaataattt acttacaaga atgaagaaat accatcatca accagttgaa caactgtcgt
     2461 ttgatgaatt aacacttatt aacttaagta aagttgtgac tgtaaatggt cacgaagtcc
     2521 ctatgcgtat taaggaattt gagttattgt ggtatttagc ttctagagaa aatgaagtta
     2581 tttctaaatc agaattactt gaaaaagttt ggggatatga ctattacgaa gatgctaata
     2641 ccgtgaatgt ccatatacac cgtattagag aaaaattaga aaaagagagc tttacaacat
     2701 ataccatcac aactgtatgg ggattaggat ataaatttga aaggagccga taatggtgtt
     2761 atcaattaga agtcaaatca ttattggcgt cgtatcgagt atactattaa cttcaactat
     2821 tttagcaatt gcatatattt taatgtggtt taacggccat atgacactaa ctttgacctt
     2881 aacgacaata attacaagct gtttaacctt attaatatgt agtattttta ttaatccact
     2941 tatacaaaaa attaagcagt ttaatataaa aactaagcaa tttgctaacg gaaattacgc
     3001 aagcaatgat aaaacgttta attcaccaaa agaaatttat gaattaaatc aatcttttaa
     3061 taaaatggct tctgaaatta cgcaacaaat gaatcaaatt aaatccgaac aacaagaaaa
     3121 aacagaactg attcaaaact tagcccatga tttaaaaaca cctttagcaa gcattatttc
     3181 atattctgaa ggactacgtg atggtataat cactaaggat catgagatta aagagtcata
     3241 cgacatatta attaaacaag caaacagatt atcaacatta tttgatgata tgactcatat
     3301 tatcacttta aatacaggta aaacatatcc cccagaatta atacaactag accaattact
     3361 tgtatcaata ttgcaaccat atgagcaacg tatcaaacat gaaaaccgca cattagaagt
     3421 gaatttctgt aacgaaattg atgcatttta tcaatatcga acgccacttg agcgtatttt
     3481 aacaaactta cttgataatg cgctaaaatt ttcaaatgtt ggtagtcgca ttgatattaa
     3541 tattagtgaa aacgaagatc aagatactat cgacattgct attagcgatg aaggtattgg
     3601 cattatacca gaactacaag aacgtatatt cgaacgtaca ttcagagtag aaaactctcg
     3661 taatacaaaa acgggtggtt ctggattagg cttatatata gctaatgaac tcgcgcaaca
     3721 aaataacgca aaaatcagtg taagcagtga tatagatgta ggaactacga tgactgtaac
     3781 attacacaaa ttagacatta cgtcataatc cgatttattt ataaaataaa atgcaaagac
     3841 taaaaagaag ctcccattaa tgagggcttc tttttttgtt tatttagaat aaactttatg
     3901 ggtatccttc tcatcatttt caagacttga aagatttgta gcttgaataa tatatttagg
     3961 acgtgcctta acttcataat atatcctgcc aatatattca cctacaacac caattgaaat
     4021 taactgtatg ccgcctaata ataaaatagc tgcaatcgtt gaaaaatatc ccggaatatt
     4081 aacaccagat atcataatat tgatgaataa atagataata tataacacgc ttattgaaaa
     4141 gataaacaag ccaagataaa tcatcattct caaaggttta ctattaaagg aaatcaatcc
     4201 atcaatacca taattaaata actttttaaa ggaccactta gattcccctt tttgtctctc
     4261 aacattttga tacgtaaaca ctttagtatt atagcctatc cattcaaata acccttttga
     4321 aaatcgatta tattcttcaa gtgatgcaat ggatttaaca gctctttggc ttaaaagtct
     4381 aaaatcacca acaccatcat caaattgtac ttcttctaca aagcaattaa ctaacttata
     4441 atacaaatgg cttaatgttt tgcgactaaa attttcacca cttctatcac gctttgcaat
     4501 cacttgatca tagccttcca taaaaccttc aaccattttc ggaatatatt caggtggatg
     4561 ttgcaaatca caatctatca tgatgactgc atcaaattca gtactatgct ggtaacctgc
     4621 aatcatagct gcttcttttc caaaatttct actaaaagaa ataaagctga catgcctatc
     4681 tattgtggca agatgttgca tttcatctat agtggtatcc gtactaccat catctatgaa
     4741 aagcatatta taatcatagc ctttcacact actatcttgt gaaagtattt cagttaattg
     4801 ttgatgtgtt tgtgtaatga cttccccttc attaaaacaa ggaatgacga ctctaatttt
     4861 catctatgtc catccttttt atttaaaaga ttttgaatgt acctgagtat tgttttatca
     4921 aatagtaaaa accaactcat ttattttaat gtacacatta aaaggcattc ataaacgtaa
     4981 aatttaacat ccagttattt taaatcccca attttcacat catctggatt tttaccttgt
     5041 cttgcatttc tatttaaagc atcaatttca gctacttctg ttaattctaa attaaaatct
     5101 aaaatatcta tattttcttg aatgcgttta ggtgtctgag attttggaat aataattcta
     5161 ttgtgtgcta aatgccaacg taatacaact tgtgctggtg ttttatgata tttttcagca
     5221 attttaacaa tgacagggtc gtctagtagt cctctatttc tcatcaaagg catccatgca
     5281 gtcactttaa tatcgtgacg atcacaaaat tcttgtaatt cttgttggtt aaaatatgga
     5341 tgtacctcaa tttgattcac cattggtttg atacttgatt gagccattaa tttttctaga
     5401 tgatgaacat taaaattaca tacacctatt gcttttacct taccttgctc gtaaagttct
     5461 tccatagctt tatatgtttc taaaaataga ccatctgctt cacaaggcca atgtattaga
     5521 aataaatcaa gataatcagt ttgtaaattt tcaatcgatt tgttgaaata ttcgaatgtt
     5581 ttctcataac cttgatagtc attccataac ttcgttgtta taaacaaatc ttctctatcg
     5641 acgccattat cctttaatgc tcgtcctagt gaagcctcat tatcataaaa gtatgctgta
     5701 tcaaacgctc tatagcctgc gtcaattgca gcatttacaa ctttagtcat atcttcgtca
     5761 gagattttat aaacacctaa cccaactgaa ggcatcgggt atccattatt taatatttgt
     5821 atctcattca acatctttat atctctccaa tctatgtatc ttttatatct ttacattacc
     5881 ctaaattttt caacaaactc aattaatacg aattatcgct ttcaataaaa attattcatt
     5941 aaatcattaa agatattgag ttccaatact attttcactt ttcattaatt ttagtcaaaa
     6001 aaataaccaa ccaaaaatga attaaatcat tcttagttgg tttatatatt aatatctatt
     6061 ctgatttttc atcttcatca gactgtccga tagtaggtct cgcttcatta aattcatagt
     6121 ttaatatcac ccaaataatt tggtggttat cgatttctga aacaacccag cgatcataag
     6181 ttgtatccac gtaatcatct ttttgtaaat tggtattacg agattgtaac catccaccta
     6241 tcgtatcaat atcctcagag tcatcaaatt ctataccgaa ctcttcagtt aaatcatcca
     6301 atagtactct gccatttact tggaatgtct tattatcaat tttaacgata tcattcactt
     6361 catcatcatc aaattcatca cgaatttctc caacgatttc ttctaaaata tcttccatcg
     6421 ttaaaatacc tgccgttcca ccatattcat ctataataag actcatatgt acatgttcac
     6481 gttgcattct aattaatgca tcactgatac gtgttgtctc tgaaatcatt ggcaactcat
     6541 gtatatagtt tgctatttta atcgtttttc cagaagcgta ttcagttaaa aattctttga
     6601 cgttaataaa tcctttaatg tggtctttat caccatcatc agtaattgga taacgcgtaa
     6661 attgatgttc ttttattgtt tctagtaatt cgtctacatt aaaaggttca tttagtgtaa
     6721 tcatttgagt tctaggtacc attatatctt ttgcatgtct ttcatcgaat gaaaagatat
     6781 tttgcatata tgccaattca gtttggttga tttctccacc attataacta ttgttaataa
     6841 taattttgat ttcttcttct gacattgcat cagtttgggc atcaggattt acaccaaaca
     6901 ttctaataat aacacgtgca gaaccattca tcagccaaat caatggtttc ataatgttac
     6961 cgaaatagaa caatggtctt gcatatacta aagcaagctt ttcagtatgt tgaatagcta
     7021 tagatttagg cgctaattca ccaagtacta catgcaaata cgtaacgatt ataaatgaca
     7081 ctgcaaacga aatcgtcgtc gttaatgcag ttggtaaatt gattgcttca aatattgggt
     7141 gtaatagctt ttcaaacgtt ggttcaccaa gccaacctaa ccctaaagat gttactgtta
     7201 tacctaactg acaagcagaa agataataat ctagattagc aatcatcttt tttactattt
     7261 tagcaggttt atttccttca tctgctagct gttcaattct tgttgctcta atttttacta
     7321 atgcaaattc tgaaccaaca aatacagtgg ttaatgcaat taatagaata aatataatca
     7381 aactaattat ggtcgaagtt tccaattaat tccctatttc tagggattca cctccatgtt
     7441 atatgtgtca ctcatgggta acacgcattc aaatttatca ctatgactta aattacaact
     7501 acatgttatc gccttcccat ttagacaccc ccagaaaaaa atgttattgc ttctatttta
     7561 tcataatata aagtgcttat gttaacagat taaatctatt gcatacattt aattatgatt
     7621 atttattagc taaatgtgtg aaccttgcct tgttttattg tagattttac attcatatca
     7681 tcatctagaa taacaagatc tgcatcctta tttactttaa tactaccttt tctatcatcg
     7741 atacctaatg caatggcttg atttaaactt gttactcgcc ataaatgatc taatgtatca
     7801 cctgtaaatg atattaagtt acgtaaccca tgattcattt ttaaaatact accagcaagc
     7861 gcaccatttg caagacgtgc ttgttgcgat tgaacagtta ctttttgtcc acccaaatca
     7921 tattctcctt caggcatacc ttttgcacgc attgcatcgg taattaaata aaaacgttca
     7981 ttacctttca tacggtaagc aattgcaacc gatgccggat gagaatgagt gccatcaaca
     8041 atcatttcgg tatgtagagc atcattcaac catgctgctc caaaaacacc tggttctcta
     8101 tgttggaatg gcgtcgctgc attatataaa tgcgtgacat gtttagctcc tcgctcaaca
     8161 gcttcaactg cttcttcgta tgttgctact gtatgaccaa ttgaaaaaat aatgtcatct
     8221 ttatacgttt caagcgcttc ttttgcacct tcaacttcag gtgcaaacgt cataatcttt
     8281 attaatccgt tagcagtctc ttgaaaatgt ttaattttat cgataaatgg gcgtacaacg
     8341 tattgcggat gttgagcacc aactttattt tcagatataa atggtccttc taaatgtata
     8401 cctacaattt ccgctgcatt gtgaacatct tgctccgctt catatttagc aatatttgta
     8461 agtgcattat ctattttatc agtcgattgc gtcattgtag tggccaagta tgatgtcgtc
     8521 ccttcagaca acaaattttc ggatagatat tttaagccat cgtatgaccc atccattgca
     8581 tcttgaccat aaccaccatg aatatgtata tcaataaaac ctggtaatac atgatgacct
     8641 ttagcatcaa tcacttgaat tttatttgtc gtatcattat caattgctgc tttatcatcc
     8701 acttctccaa ttgcaacaat ctgtccatct ttcacatgaa tgtaaccatt atcgattttg
     8761 ccatcttcag tataaacttt gccgttatat ataattaatt ctgacacgta taaacccttc
     8821 tttatttata aattttcact ttaagacact catattttac tatatctgct tcgtttggca
     8881 taactttttg caaaatatta aaaaagtcta tacatttgtg gtgtctattt cttcattcat
     8941 ttatctaaaa ataaaaacac tttcatatca attcataaaa actgaattaa tagaaagtgt
     9001 ttttacagct ttgtataagc taccgttaac acgatatata aaactaacat atattcattg
     9061 aacatacaac ttaatatgaa gctctttgct aacaatcatt ttacatcatt aaaactactc
     9121 gtccattgat tttgaagctt cgatttctgt ttcagttaac tttggtttga ttaaaccgta
     9181 aattaatgct gaaactaatg tgccaactag aagtgcaata agagtttgaa gtaagtgtgc
     9241 accatcagta ccaacaatta caataatacc accatgtggc gcagtaattc gtgaacctaa
     9301 gcctaaagca attgcgccac ctatacctga accaatcatc attgaaggaa taacacgtaa
     9361 tggatcggca gctgcaaatg gaatcgcacc ttctgtaata aatgacatac ccatcacata
     9421 gttagggata attgaaccac gttgttcttt tgtaaattta cgtctaaaaa ttaacatcgc
     9481 tgtcgcaatt gctaacggtg gaatcatacc accaatcatt gcagctgtaa ttggtgctgc
     9541 attaccttca atcaacgcac ctgttgcaaa aacatatgcc gctttgttga atggaccgcc
     9601 catatcaatc gccatcatag cgccaataac taaacctaat aatacaatat tagaacctga
     9661 taaattgttt aatccatcta ataacaaatg atttaaccaa gatgctggtg gattaaaggc
     9721 ataaatcatc aataagcctg tagccgtcac tgttaatagt ggataaatta atgttggctt
     9781 taatccctct aatgcttgtg gtaacttacg tgtcatggct ttaacacctt gtgttaagta
     9841 acctgctaag aaacctgcaa taataccacc aataaatcct gaaccacctg aaattgctaa
     9901 cataccacct acaagacctg aagcgaaacc aggtttatcc gcaatactac gtgcaatgaa
     9961 tccagataaa attggaatga ttaacgcgaa tgcactttta ctaccaatgt tccaaagctg
    10021 ctctgcaaac gcattgtact ctgagctttt tggattaaat gaatttgctc caaataaaaa
    10081 tacaattgcc attaaaatac caccagatat tacaagtggc aacatgttag aaacaccgtt
    10141 cattaagtgt ttatagaatg ctttacctgg gcttaatttt tcttgactgt catttgagtt
    10201 accttttctt tgaccatcac gggcaacaaa aggtttacga cttgtatcta atgctttatt
    10261 aattaattct tctgggcgtt taataccatc tgctactggt acttctacga catttttacc
    10321 atcgaagcga tccgtctcaa catgaacatc agcagcaaca atgatacctg ttgcattttc
    10381 aatatcttgt tcagttaaat ggtttttaat gccgcttgaa ccattcgttt ctactttaat
    10441 tttaataccc attttatccg cttgcttttt caatgcatca cgtgccatat atgtgtgtgc
    10501 aatacctgtt ggacaagcag ttactgctaa cacatatggc tcattagatt gtttagatga
    10561 ttgttctgca gttgcaactt gttgtgcttc agcttctgcc tcttcttctt ttgtcacttc
    10621 atcatcagcc tcatctatga tcgctagtac ttcttcaggt gatgaagcat gtaataattt
    10681 ctcacgtaca ttttcatcca ttaaaatacc agacaactta gctaaagcat ctagatgtgt
    10741 ttgggcgcca ccttctggcg ctgcaatcat aaagaataag tgtgctggtt gcatatccaa
    10801 actttgataa tctacgcctg ctttagattt accaaacgca atagctggtg acttaactgc
    10861 ggccactttg gcatgtggaa tggcaatacc ttcgccgata ccagttgtac tttgtgattc
    10921 tcgattgtga atcgcttcct taaatgacgc gacatcactt aatttacctg ctttgtctaa
    10981 ttgatttact aactcatcaa taacaccatt tttgtcattt gccattaaat ccattgctat
    11041 tgtatctttt gttaataact ctgttactct cattattttc actccccatc aagtacgcta
    11101 atcgtaactt gtgattttat tttttctata gcgtcccgtg ttgctaagtc ctcatcaaat
    11161 gccgtggcag taccgcatgc gactgcttgt tggaatgctt tttcaatcgt taaacctgaa
    11221 gcaattccag ccaccatgcc tgcaactgta ctatcaccag agccaactgt attaaccact
    11281 ttcccttgtg gattaactgc tttaatactg atttctttat caatataaat agcaccatca
    11341 ccgccaagcg agacaataac agattgcgca cctttatcaa ctaacaaacg accatattta
    11401 ataacatctg tgtctgagtt cactgttgta ttaaacatca cttctaattc atctttatta
    11461 ggtttaataa atagtggatg atatggtaaa acgctttcag ccaattcttt ttcagcgtcg
    11521 actactaatt tagcacctgt ctgtgctgta atttgtgcaa tttgcgcata cgcatcgctt
    11581 ggaatactac ttggtacact tccagcaaca ataactatat cttcgcttgt tgtattttta
    11641 atttgttgta acagttgttc aaattgtgtt gacgttatat gaggacccgg tgcattgatt
    11701 tctgtttctt gtcctgtttt taatttcaca ttaatacgtg tatcttcatc aacttcaata
    11761 aaattcgatt gaattgcact gttatttaat gtatctataa tgaatttccc aggaaatcca
    11821 cctgcaaatc ccaaggcagt tgactcaaca tccaatgtct ttaagacgcg cgagacatta
    11881 atacctttcc ccccagcgaa tttatatgtt gctgttgctc tgttcaaacc atcaatttta
    11941 aaatcattcg taaaaatgac atagtcaatt gaaggattga aagtcactgt ataaatcata
    12001 aagtccctcc tataaagtga tacttttgtt ggtattcttt taacgattct tgatttaatg
    12061 ctttttcaga tgtgatgatt gtcgtacttt ctagcaaagg tacacgagca aaatatactt
    12121 tattaaactt agaatgatct ataagtacaa atgattgatt ggctaatgac attgctgttt
    12181 gtttaactaa tgcctcttgc tcatcgggag tagttaatcc aagttcaata tctaatccat
    12241 tcatcccgat aaaagcttta tcgaaacaat atcgtcttaa tatctccata gcactagaac
    12301 caatcgtagc aagtgtattt tctttaactt gaccacctag cataattgtt ttaatacctt
    12361 ttttaagtaa agcttctaca tgtgttaaac cattagttac cacaatgata tctttcgctt
    12421 gaatatattt aattagctcc aatgtagatg aaccagcatc gataaataag cattcattat
    12481 cgttgatttg attagctgct attttagcaa tcattttctt ttcatcaaga ttcgttgcta
    12541 atttttcagt taaattcgcc tcaaccatac gattttcttt taacattgca ccaccatgca
    12601 cacgttgcag tttccctaat tgttgtagtt tagataaatc tcttcgtatt gttgaagcac
    12661 tgcaaccagt tcgatctatt aattcttgta aagtcaaaaa atctttgtgc gaaagttctt
    12721 ctaatattaa ctcgtgtctt ttttcagtaa ttatcatcat caccccatct cactttgtat
    12781 tataatgagt acgctttcac ttttcaatca taaattacca aaaacaatca caaacactca
    12841 aattgtgacg ataaaattac atttttatgt tcaaatactc aattataatg actgtttatg
    12901 atcgtaaaat gctaattcaa tcaaaaagga gtgtggcaga aatgaatttt ataaaaattt
    12961 catctctgtc acactcctaa tcttgcccca tactttttgt tgttattaat cattcatgga
    13021 taactgctcc aattttgcct ttagttattg taatcaaatc ctcaacagct attgtgattt
    13081 gcattgttcg aagcccacca ctcacactga tatgactata attttcacac gatttgtcaa
    13141 cgactgttgg aaacaatgtt ttcataccaa caggcgaaca cccaccacga atgtatcccg
    13201 ttacattttt caaattatct aaaggcataa gctgcaattt cttctctcca accaaagcag
    13261 ctgccttttt catatctaaa gtttcactta ctgggataac aaatacaaaa tgttcatgtt
    13321 ttgtattttc tagaacaagc gttttaaata ctgttttaac atttgcttgt atgagttctg
    13381 cgacttcttg accagataaa tgttcttctg gtacctcaaa gctatgaact tcgtatttaa
    13441 tttttgcacg atcaagcata cgcatcgcgt tcgttttctt atttttagcc atttcaacac
    13501 ctcatttata cttcttcaga actatattaa cattttaaaa tacattacgg attggttgat
    13561 tttgacataa catttacatc attttataag ttgaattcac ctttttaaat taaattattg
    13621 caaaataaaa acagtatatc caattagaat acactgttcc atctattatt tcgttattat
    13681 gccttgattt gaaatactta catgttgctt actagttgaa ttatcattgg aatacacata
    13741 attatatcca tgatttgtct tagaaattct ttctaaacca taattatcat tgatatgctt
    13801 atctgcataa cgatcagcaa taacttggca ctcttgttcg ttaaaagaat ttgatttatt
    13861 tgcattcaca cgatgtttaa aatgattttg ctttttcaca ttttcattca cttcagatgc
    13921 tttagcagta tgaatattga gaatatccat tttcttttta taagtatctt cagttgatac
    13981 atttatcgcg ttaagcgtat taattgtttt atgtacctta gcaagcgcat cttcatttgt
    14041 tgctaatatt aataaaccaa ttaaacaaac ggttaatatt acagcaacaa agcctaaaaa
    14101 taaatttttt actgtcatcc ccttacccac atttccttac tcatttttta tcatcttaac
    14161 acattaacgt taatgaatta ggtatgtgga ttgcaattta aaaagaatgt aatatttgta
    14221 acatgtttat tactcaaaat cattttttgt tacatataac atttaaatat aaagagtaaa
    14281 acatttagtc aatcgaatct ttttaaatgt aaagcatcga attttcttaa taaaattcat
    14341 aagaaaaacg atgctaatca ttcataatat tacgctttac tttatatcaa tatacaccaa
    14401 aatacttatg ctacatattt tgttctttca attttgctct atgttgcttt tcaattaaaa
    14461 caataacaac acctgctaat gaaacaccta tagccatata aattggtgct tcaatgtgta
    14521 catcaaataa cgcacctgcg attaaaggac ctatgaaatt acccatacta gtgaatgtcg
    14581 agttcaatcc gcctgcaaag ccttgccttt ctccagcaat attagaaaaa taatttgtaa
    14641 tggctggtcg tatcatatca aaacctatga agacaacaaa actgattaac attattgacc
    14701 aatagtcatt agcaaaaact aataatatta agacaacaac tgaatataat aatgaccaag
    14761 ctataaatgt taactctgag aaatacttca taaatttatc gaagaaatag atttggaaaa
    14821 gtgccccaaa tataccgcca cccgtaatag caatcgaaat atctttaggt gaataattta
    14881 ccttgtcagc tgtgtatagt gaatacaatg tttcaaatgc agataaacca aacgataata
    14941 caagtgttaa aataactggt gtaataaaca ctttccagtt aattttcgtt agcaattgtg
    15001 gctctaactt ttggaaacca cttgtcgtag actttttcgg atcgtgaatc aatacaattg
    15061 acattataaa tgctagaata cctaatgctc ctgcaaagta aaatggcata cgatgtgaaa
    15121 cttctgccat aaatccacca atccctggtc ctaaaatgaa tccagaattg ataatcgctg
    15181 acatgtagcc aaagtttttt gctttttgat ggcttggtga aatgtcagct attaaacctg
    15241 tcacaccagg cattaccata ccagcactca taccaccaat cactctcgat aacatcaata
    15301 ccgaaaaatt gtggccaact gcaaacataa attctgacac tgaaaacaaa attaatccta
    15361 tacatataat taatttcttc cctaatttgt cagctagcgt accaccaaac ggcgatataa
    15421 tcatttgaga taacgcaaaa gcagcaacta gtaatcctaa atcactacca gttaatccca
    15481 aatcttttaa ataaacaggc aagactggta ttactaaacc gatacctaaa aaaatcaaga
    15541 aaatattaaa atataagaca aaaatctgtt tattcatatg ctcacctctt ttttctccat
    15601 gtcatgctta aagctgttat tcttcttttc cacgacagat tgcaattcat gctaaatatt
    15661 cgtaaaaagt ttctatattg tattacaaca ttgctacatt tgacaatatt ttttctttgt
    15721 aattatcact attttccatt taattgtata attaatgaca tattaaaata aaggggattg
    15781 atcatatgaa attgcaaaaa agtaatcata ctatactact agtgttggaa aaaggagaag
    15841 atatcgttga atgtataaca acctttgcag atgaccaaga cttaacattt acatctgtca
    15901 gcggcattgg tgcatgtgat gacgttgtat taaaattttt caatttaaca acgaaacaat
    15961 acgaagaaaa acatattact gagccactgg aattgacgag cttattaggt aatatttctc
    16021 gtctagacaa tggtcatttt gcacatttgc atgctacttt cggtactcaa tcatatgaaa
    16081 cttttagcgg acatctcgct aaagcaattg tttctgcaac tgctgaaatc attcttactg
    16141 taactgattt ggacattcaa cgttcattca aagatgcagt cggtttaaat ttacttgatc
    16201 ctcaatagca ctttactttt tagttgaatc aaattttatt acaagttatc ataagtgctt
    16261 aatcataaat gcattctcgt ttattaatat acttcaacga gttaaattaa atttttaatt
    16321 aggaattgaa tgcgtttata actatcacat ttgaatgaat aactttttca ttagaaagta
    16381 gtgtttatta ttgatgcaat ctcgatacat cgctagaatt tactttttaa ttttatttat
    16441 cgtctccctt tttgagacta aaatttttca atttatgact ttaaatttgt ttttagcata
    16501 tatacctttt gagcttagtc tattattacg attattcaaa ccgaataaaa agtatgaatg
    16561 gccactcttt atcatatata gttttatatt tgtgttactt ttaccaaata cattttatat
    16621 ggtaactgat ttaatccatt tgaaccaatt tcaattcaac ttttacgcag gactaaattt
    16681 aagtgagtgg aaatacttta cctatttact gcttggtgtc ttcttagcgg tttatgtgat
    16741 gattttaatc tattttgaaa tactaacatt gacttctcac cgttggctta atagattaat
    16801 tgttgtcgtg ctaatgtttt taaatggatt cggaatttat attggtcgtt tcttacgctt
    16861 acattccgtt tatttcttta atgagccctt acatgttcta aatcacattc tagaaagtct
    16921 aacgcttaaa actgctatgt ttgtatgttt tatggttgtc atgcaagcag caattctatt
    16981 atttggaaaa ggagtgcgtt tgaacaagtg acaataaata taatcgtttt aatagtatct
    17041 atcattgtct ttcaattgat tattggacac atatggcatg atattggttt aagttattta
    17101 agaagtatct tactcatgat gctgccattt ggactaggtg tatttataca acaagtgtcg
    17161 tattacgaac gacaatatcc taaatggcaa gtgcctcaaa acataaaagt tagattgaaa
    17221 tatatttatc tcgctacgtt tttagagtat gtcgtactat acctcacttt atttacggac
    17281 atactaagat aacatcaaat taataagaaa actaaataca acttatatat ttgaggaaag
    17341 ccaatttcta tcacatcaat aatagaaatt ggcttattta tattgcttaa gaactttata
    17401 ttctgctcca gattttactc atgccagatc tatcaattga gccgaattag tctagcgctt
    17461 taaatgtagc taaaacttgt gtacgttttt cttgatgatc taccatttgt cttggataat
    17521 gactacctaa ttcaatcccc tgttcaaaaa ggttggactc attgcgttgt gtatcatgca
    17581 aatattttgc atcaatttga ttaaaaatcg gaagatatgt tttgatatac aaagctttag
    17641 catcaaagcg ttcactctgt cttattggat taaacattct aaaatacggc actgcatccg
    17701 tacctgtaga agcagaccat tgccatccat gaatatttga tgctgcatca tagtcaataa
    17761 ggtgctttct aaagaatttt tctccccatg tccaatctat aaataaatct ttggttaaaa
    17821 attgcgacac aaccattctc attcgattat gcataaaacc agtttgtgtc aattccatta
    17881 ttgctgcatc aatgattgga aatcctgttt gcccttcgca ccatgcatta aaatccgctt
    17941 cattttgcga ccattttatc tgtcgatatt taggtttgaa agcttggtat gaggtttcag
    18001 gatactgtgt cattaacaca taataaaatt ctctaaaaat gagttcacgt ataaatgctt
    18061 catagtttgc ctcatcttca tcataacgcg ccaataaatc attaaaaatt tcaataatat
    18121 ctaataatcc atatgctaaa gcaacactta gctgacttgt taatacttca ggtaaatagt
    18181 ctcttccgct ttcgtaattc tgtatatctt gatctaaaaa atgttgccat ttattctgtt
    18241 caattatttg tgaggatcca aatgctatgt catcaaacac taaatcatca ggtgatgcaa
    18301 tgacaaagtc ttcgaatgat tttaaatcat agtgatatac gtctctatgc ctcaagtaag
    18361 gtcgccattt tttatagaaa ctagtaaaaa cattgtatgg ctccccttgt tgattcattg
    18421 ttttactcgc tttaaagtaa tgattcccac gtatcaaagt aacggcaatg cgtgcttcat
    18481 tgaataaaga acgttgatgt aaaatatcat attcttcaag atgataactc ataatatcac
    18541 ccgcagtcac tacttcagag atattgtttt ctttacaaaa tgaaatgata tcgtcataag
    18601 acataacata aggtgtaagc ttttctgcgt gcaacgattg gtaaaagcgc tgcaaagtac
    18661 cgcgataaaa ctgtgctttc aaatcagaag ctgaagacat gtcctcttcc ggaatgataa
    18721 aataacatgc atcaatgtct tctttttgtt gataaatata ttcaaataat ggattgtgtt
    18781 ccattcgaaa cattcgattt aataacacag caattgccat aaacaaaaca cccttcaatt
    18841 atctttttat tttattaagc tttttgtcac tttaaaataa cctagattct tatttatatt
    18901 tttaattaaa gcaaacctaa ccatttccaa taagtcatgc taaaaactat cactagaata
    18961 taccctacaa ttgtcaatgg tatacctgcc ttcaagaaat ctttaaccgt aaaagtacca
    19021 gtgccataag ccaacatatt ttgaggtgca ctaacaggta ataagaaacc aaaactaata
    19081 acaaattgtt gaattaaaac aaatcctata gactggtctc ctaagtgtaa cgtagaggtt
    19141 agcgaaataa aaacaggtat taacgctgat gataaacttg ttgcactcgc aaagcccaaa
    19201 tgaatcaata tattaaaaag cgtgataagc gcaattgtcg cgataatagg taaatgtttt
    19261 aaacctaaaa caccaaaagt ttgatcactt aaccattgag ctgcacctgt tttcaaaaga
    19321 acgttaccta gtgaaatacc tacaccaaac acgataattg ttccccatgg tattttattt
    19381 tcaacatgtt tccatgtcat gacaccaatt ttcggcatta acataacacc taaagcaata
    19441 atagtaatgg atgcagagtc aatcggatgt aatacttttt cagttgacca aaacagtaat
    19501 aataacatcg atataacaat taaacgccat tcacgtgggc taacggggcc aagtttatgc
    19561 aattcttctt ttattaaatc tttaccacct tctattgtat taatttctgg aggcatcact
    19621 ttaatcatga tgaaatataa agctacggac ataactatgg accaaggcgc tgcatataag
    19681 aaccactcgc cccatgaaac atcaaatcct aattgatggt ttataaaatt aatcgctacg
    19741 atattttgtg ctgccgccgt tttgatacca atattccaaa ttgacacagc ttgtactgaa
    19801 gtaattatta ataaagacgc taacttgcta tctttggaaa ctttaaatgc cgcaatcata
    19861 cccagcaaga ttggtacaac tgcccctgct ctagctgttg cagaaggaac gaaaaatgca
    19921 agtacaattg aaacgataat tgctccaata actatatttc tagttttatt accaacaatt
    19981 gataacacta aaagagctag tcttttatgc aaattcgttt cttgcatagc agcagccaaa
    20041 aataatgcag ctgcaacgag agctacagct gaagttgcaa atccactaaa cgctaatgat
    20101 aatgcatgat tagttcctag aaggtcactt ccagctaaaa tagcactgcc acttttcgga
    20161 ttacctagct tctcccctaa attttgaaca gggctaaatc ctaaaagtaa tatcattaag
    20221 ccaataatta aagttgctga caccggatat gatacagctt ccgttaccca cataataact
    20281 gcaaaagcta aaatagctag tactgcctta gccattatcg gcagacttga aggtgccggg
    20341 agtaatagta caactataag tattataaaa ctcatgacaa tccaaaatgg cttgtattgc
    20401 ttttttgtct tttctttctt ttgttgttca tgttcaacta catgtttttc ttctgacata
    20461 acagtcaccc ctttgtttaa tcttaattta attataacaa ttttttaaaa catacatttt
    20521 taatttatgc aaaatacaca attttatcga aatattttgt aaataatatt gctaggtgat
    20581 gagttatctc ggttaattac aaaacgatat ctaagtttaa atattagatc ataaaaaaac
    20641 tatctactga atatcctcat agcatcattt ttctaagcaa aagttaaact tagttagata
    20701 aaatgcaaat gagattattg tagatagtct ctttttaatg tttaaaaatg attaatgcca
    20761 taaacataaa taattccaaa gaatgccgcg ccactaagca ctgttaatat aagacttagt
    20821 aacaatgtgc gtttataatg ttcaacaaat gctatgaaca aaatgactac attatacgca
    20881 aagaaaagtc cgaataatgt cattgttatt ttcaaatcag aatagaaaat atttaatagt
    20941 aatacaatga ctgcataaat taaatacgga ataactataa atttgcgttc aaatcttaat
    21001 tgttcgaaac gtttatcttc gtttgtcatg atatgctcct ttatgattta aaagtaaata
    21061 agtttacgga atatcttgta cgataatagc ggtgtaaatg tcaaaccacg actgatcatc
    21121 taaattaagt tgtagccctt cgattgcttg atcaatacgc tttaactgac ttgttccaag
    21181 tatcggcatg atacgatgcg gtatttttac taaccacgct atcatcacag ctgtgtcact
    21241 cacaccatat ttgtcagcta ttgattgaac aactttcata atacgttgcg ctttaatatc
    21301 ttccttgtcg aaaattttac cgcctgcaaa aggactccaa gccatgattt gaacatggtt
    21361 ttgatacatt gaatccattg ttccatcttg taaactatca acgtgatatg gcgataattc
    21421 taattgattg atgctaatat gtagtctttc tttcataata tattgattta acaattggta
    21481 ttgtgaatga ttaaaattcg acaccccgaa tgacttcaac ttaccttgtt taacaagttt
    21541 agttaatgca tcagcaactt gttctggatc catcaatggt gaaggacgat gaatgagtag
    21601 actatctaaa taatctacat tcaaattgat taatgactgt tcaacagatt tcacgatgtg
    21661 cttactactc aaatcataac gatgtccatt tgtaaaatca aattgcttag aaggcaaaat
    21721 gataccacat ttcgtaacaa tttgaatttt atttcttaat tcgggtgata aatccaaagc
    21781 attaccaaac agtgattcac attgataatc tccataaata tcagcatgat ccatcgttgt
    21841 aattccacgt tcaactaatt catttataaa ataatttaac tctttcgcag tcatcttcca
    21901 ttcatttgca cgccaaaaac cttgtacaag cctagaaaaa tgaacatagt gattaatcat
    21961 tatttgttcc atatctcatc cactccatcc ctcaatgtca caaaatattc acaataaaag
    22021 tattaagaaa ttaatgtgct atttccgtta aattgaaaca acacattttc tgatttacgt
    22081 aaattgtagc atatccaatt gattacgtat gtgatttgta tctaaagttt caccaataat
    22141 aacaatcgtt aatggtaatt gcatgccaat tattccatag tctggtaaac catatgcata
    22201 ttgaaattca taaattgcat ttggttgatc tctaaatgac acataacctt tcaaacgtag
    22261 aacagattcc ggtaatttca ttataaattg ataaaacaaa tgacgatcaa tcggacctgt
    22321 aaacgtgtaa gtcatacttt taatcccatg atgatggtga tgatgagacg atatctctct
    22381 gtctttaaca gttaagtcca attcttccaa cgaaactttt ccataggttg ttttataagt
    22441 tggaatactt gcacaaatca tacctaattg cttatcaatt ttctcaagac tgtcatcagt
    22501 tataagatca attttattaa taataatcat gtcacttagt ttcaactgat cttccatcag
    22561 cgaaactgta ttttcggtat attgatgacg ttctaaaaat cgagtcgcat ctaatacacc
    22621 ataaataatc ggcttttcaa agaaattaac gatttgcgga tcttgacatg caactagtaa
    22681 ttccaaagga tgcgcaatgc ctgtcgcttc tatgatgaca tgattaacgt ccccttttaa
    22741 agcaatggct tttagttcat agacaagttc ttgtttaaga tcgcaacaaa cacaaccatt
    22801 aatcaatgaa tggacttcaa tttcatttga aatactattg ctatcaatat caaaagtacc
    22861 gaattcattc atgatgattt taattttctc atcatttttt aataattcac taatataatg
    22921 tgtcagtaac gtggttttac cactacccaa aaatccgtta attatggata ttcttatttt
    22981 ttcatcttta ttatttttca ttttatattc tcttttctat tacagttatt tttaaaaatg
    23041 ataacatata tattaaatta tctttttgct tgatttaatc aaaaatatct tctaaacttt
    23101 aaacatactt taattttagc atggcattga atcaaagaaa gttgtgaatt aataaacaat
    23161 caacttttta atgattacca atccgaaaag agaaaaaaac ggatagtatg tctggaggag
    23221 aactttaatg tctgatcaac ataatttaaa agaacagcta tgctttagtt tgtacaatgc
    23281 tcaaagacaa gttaatcgct actactctaa caaagttttt aagaagtaca atctaacata
    23341 cccacaattt cttgtcttaa caattttatg ggatgaatct cctgtaaacg tcaagaaagt
    23401 cgtaactgaa ttagcactcg atactggtac agtatcacca ttattaaaac gaatggaaca
    23461 agtagactta attaagcgtg aacgttccga agtcgatcaa cgtgaagtat ttattcactt
    23521 gactgacaaa agtgaaacta ttagaccaga attaagtaat gcatctgaca aagtcgcttc
    23581 agcttcttct ttatcgcaag atgaagttaa agaacttaat cgcttattag gtaaagtcat
    23641 tcatgcattt gatgaaacaa aggaaaaata attaactttt gtcatgacaa ttaaagtaat
    23701 gtttagaatt tattaagaat agaaaaacaa ttagcacgcg taagcttgtt agttaaaaaa
    23761 ctgcttgaaa ggtttcttag cctatcaagc agttttttta tgcattatat tgaatcatat
    23821 tccataaagc accttgatta gctagtaatt gttgatagtt tcccttttca actattttac
    23881 catttatcat cacaatgatg gtctcaaaac gtgacaataa agttaaatcg tgtgtagcaa
    23941 caattaatgt ttctgcatgt gcttcaatta aatccataac ttttaaacta ttttgtttat
    24001 ctaatgcagt tgttggttca tctaaaatcc atgttgatgc agtatctttt aataacatcc
    24061 tcgtaatcgc taaacgctga atttctccgc cagataatgt atgaccatct aagtcaattt
    24121 gacgttctag tgccaaatgt tctaaatcta attgcttaaa tattgcttgc accgcttcat
    24181 ctttttcatc ggtaaataaa ttttgacgta ttgtaccatc aaataattgt tgagattgta
    24241 gcaagacatt taacgattca aacttatctt tgtcatctat ttcaaacata tccatatttt
    24301 cgaaacgaac agagccacta tctaattgat ataaccctgc cataatttgt agtaatgtac
    24361 tttttcctga accagaagga cccacaatcg caatcttttc gcctctatta acattaaaat
    24421 taatatccga caacacatac gtttgctgat tccaatactt aaaactagca tccttaactt
    24481 cataaatgtt cgttgcatca tacttatcat taagacgttt ttttccatta gtagaaggta
    24541 cagatatcac ttcattaata tcgtgcaatg cttggtcagt atccgcttta taatacgcga
    24601 catttgtcat tggtacagct tgttcaaata aagttaagac cattaaaact atactcgtca
    24661 tataaataat atttagttgg cctgcattaa tttgaattaa tcctagaact aaactaccaa
    24721 aaatcgaaag catagcaata atatttaata taaaatcata aatcgttaaa aagcgttgct
    24781 ctttaagttg taatttatca aaatgattta atttagccat caaattatct cgataatgat
    24841 taatttgatt aaatcgacgt agttcatcca taccagcttt ataatcataa aaatgattta
    24901 aaaatcgggc ctgttcatta gctgcatgtt tctttaaagt acgtgctttt tttgagctta
    24961 accaaggaac aatgagtaaa gtgaacaaca tgctaaccat aatcaatagc gcatggccga
    25021 ttgaaatgaa cgccaaaact atgactgtaa ctagcgctgt caaaccgatg acgactggtg
    25081 gataataaac acgtaaatat atattttgta atgcctcaac acgactaatc atacgtgaaa
    25141 ttaaatcact agaactcagt ttacggtaaa cattaggaat gacatttact aatttaccga
    25201 aaaactgtac ccgaatatca cgtagcattg taaatgtagc tttatgagaa ataaggcgct
    25261 ctacgtatcg agtaatagct cttaaaaacc caaacaattt tactgtaacg actaaaatca
    25321 tcagagcgta tagtggcgca ccaagtgcac tttgtgtcac catataacca cttaagaaaa
    25381 acatggcgag cgcaactaaa cttccacaaa caccaacaac tatagctaac aataaatcct
    25441 tatctacttg aaattttagt cgtgttttca taagtcatca ccattttctg ttatatttac
    25501 tgaaatattg cgatcatcag caatcagtct tccattttct atatacaagc gtcgttgtaa
    25561 atggcgaatt gtattatctc tatgtgcaat gacaatcatc gttgtatctt taaaatgttg
    25621 aaacagaacg ttctgaatca tgtgttctgt ttgaatatct aaaccagttg caggctcatc
    25681 aaatataacg agatctggct tcataactaa aagacggcaa agttctatgc gtctcatctg
    25741 tccaccagat aacatttcgc ctccttcacc tattattgtg ttaatgcctt ttgtgaaaga
    25801 ttgcactttg tctaataaac ctacttcgtc tagcacttct tcaatagtat tattttctat
    25861 atctttaaac atcgtaatat tctcttttat agaagcactg aaaatatatg gctgttgact
    25921 aagtattcct atatttaaat cacgctggtt tgtacttata gtacctattg ttggctgata
    25981 aacacctgca ataagatgtg tcaaagtgga tttacctgcc ccgcttggac ctacaagtgc
    26041 aatttgatca cctttaaata tttccaaatt taaatcattt aataccaatc tatcagaatc
    26101 atcatatcga aatgatatgt ctgttaactg aataaatggc ttttggtttt cctcatactt
    26161 taatagaaat tcattattat agttcggttg ttctaaaaac tcaaacacaa cgtcactggc
    26221 accttcactt tgttttccag tatggaacgc ttgccctaag tccttaattg cattataaaa
    26281 ttcaggcgct aaaataatcg caattgccgc agttttaaaa tcaatattat gaaatactac
    26341 taagcttagc gttgcttcca atgcaaccaa tccaatacct aacatactta taaattcgag
    26401 cattaatccc gataaaaaag cactgcgtaa aatgcgcatt gttaaagttc taaactgagt
    26461 actatcgtcg taaatatgct tctctgtttg ctctgtacga ttaaatagct ttaacgtcac
    26521 taaaccttta gcaatattta aaaaccgttg actaaattga ttcaaataag tcatttgatc
    26581 ttttgactca tctcgcgttt tcaaaccgaa aataatataa aacaaaggaa taaatggtgc
    26641 agttattaac ataattaatg cggtattgaa atggatgaaa aacattgcaa tgattatgat
    26701 gagcggaacc atcattgatt tgaacacttg aggcaaataa ctcttataaa aaggtgctag
    26761 accatcaatg ttttctgtga gtatagtcat ttgttcaccg attggatgac cattattttt
    26821 ataaataacc cgctgtctaa gcatatgctt aactttaaat gctaatgtat cacctaacca
    26881 ttgatttaga aattgcacag ttgctcttaa aagtaaaaca cctaataaaa taaataatac
    26941 aatccataaa ccttgaaatt gatgtcttat aattttagct aaaaaatctg ctattaaaat
    27001 attttgcgtt ataacgatta tgccgagacc agtactgacc aagaacatga gtaccggaaa
    27061 aattttatat tgaaacagta ttgttgttaa ttttttcaca attatatcac ctaaacctat
    27121 tataaaaggt tttccttaaa atcaaattaa atagctcaat aaaatattgt agttttacat
    27181 gtaaaagaaa atgaatatat acatctttag tctagtatta taactagcat gtctttaaat
    27241 gaatatgcta tcatgatatg taattttgat atttaaataa attaaattga gtaatgaaag
    27301 tgggtacatt atgtttatca ttgaattaat aaaaggtatt atcttagggg tcgtcgaagg
    27361 attaacagaa tttgcacctg tttcctctac tggacatatg atcctagttg atgatatgtg
    27421 gttaaaatca tctgaatttt taggttctca gtcagcattt acatttaaaa tcgtcatcca
    27481 attaggatcc gtctttgcag cagcatgggt gttccgcgaa cgcttcttag agattttaca
    27541 tattggtaaa cacaaacatg ttgaaggaga taacgatcaa caaagacgtt caaagccaag
    27601 acgtttaaat ttattacatg tattagtggg tatggtgcca gcaggtattt taggcttact
    27661 atttgatgat ttcatcgaag aacatttatt tagtgtgcca actgttatga ttggcttatt
    27721 cgtaggtgct atctatatga ttattgctga taaatactca gctaaagtta aaaacccaca
    27781 aacagtagat caaatcagtt atttccaagc atttgtaatt ggtatttctc aagcagtagc
    27841 tatgtggcct ggtttcagcc gttcaggctc aacaatttca actggggtat taatgaaatt
    27901 aaatcataaa gcagcatcgg actttacatt tattatggct gttccaatta tgttagcagc
    27961 tagtggttta tctttattaa aacattatca agatattcag attgctgata taccttttta
    28021 tattttaggc tttttagctg catttactgt tggactaatt gcaatcaaaa cattcttgca
    28081 tctaattaac aaaatcaaac taattccatt tgccatttac agaattgtat tagtaatctt
    28141 tattgctata ctttacttcg gatttggcat tggtaaaggt atttaatttc cttttaaaat
    28201 atagactgtc aattaaggaa tcacttgatt ggcagttttt attgtcttct tttaaaaaac
    28261 atctgaaata cttaatagtg attcactact atcatcgttt atattgatgt atcatagggt
    28321 atgtaaaatt aataaagtaa ttatggaggc aagatctatg gataagaaaa aagtcatcaa
    28381 atttatgatt aatgtattac caattgtatt ggtaccgtta attgttgaac gtaaacgtat
    28441 caaacaacat ccggacgtac aaaaagttac agatgctaca agtaaagttg cttcaaaaac
    28501 atctgcagca atcagtaaca cagcgagtga tgttaaagaa tatgtcggcg ataaaaaaca
    28561 agattttgaa aataaacgtg aacttaaaaa gtttgctaga gaacatgatc ctgcctatat
    28621 tgagaaaaaa ggcgaaaaat tagctaaaca aaatcgtaaa gacgctgata aaatgaataa
    28681 aatacttcaa aaaaatatcg aaaagcgtca taaagaagag caaaaagccc gcgaaaagaa
    28741 tgaaatacaa cgtattaaag atatgaaaaa atcacaaaaa tacgaagaaa aagcaggctt
    28801 aacacctaat aaattagatg agaaaactga gaaaaaaggc gataaactag ctgaaaaaaa
    28861 tcgcaaagaa atcgctaaaa tgaataaaaa gttacaaaaa aatattgaaa aacgacacaa
    28921 agaagaacaa aaacgccaac aagaagctga taaagcacgc atcaagtcat ttaaaaaata
    28981 taaagattat gttgccaaaa gcgcctctca acaaaataaa gaaaacaata cagaggcata
    29041 attgtgacac atattattat tgatggagat gcttgtcctg ttgttgattc tattatagat
    29101 ttaacaactg agacaggcat ttttgtgaca attattcgga gcttcagcca tttttcgaac
    29161 caattatatc ctccacatgt atcaacatta tatgttgatg atggaccaga tgcagttgat
    29221 tacaaaattg ttcaattatc aacgaaggat gatatcgtag tcactcaaga ttatggactc
    29281 gcaagtttat tagtcgacaa agtcttaatt gtcatgcatc ataatgggaa gatatacaat
    29341 tccaaaaata ttcaacaact attagataaa cgatatatga atgcacaaat tagaaaacaa
    29401 ggtggtcgcc acaaaggtcc cccacctttt acaaagcaag atcaaaaagt gtttgaacaa
    29461 tcactattaa aagtcattca tcgaattaag gagctggatt aacatgaaac gaatcgcagt
    29521 ttattgtggt gcaagcaaag gtcatgaccc ttcatatgta caaaaagcat acgatttagg
    29581 taaatatttt gctgaacaag gttatgaatt agtatttggt gcagggtcaa ttggcattat
    29641 gggtgccatt caagatggtg tattagatca tggcggcaaa gctattggcg tcatgcctaa
    29701 aatgttagat gaacatgaaa tcacaagcca acgtttaact gaattaattt tagttgattc
    29761 tatgcatgaa cgtaaaaata aaatggcaga acttgcagat gcatttgtca tggctcctgg
    29821 tggcgcaggt tcactagaag aatttttcga aatgtatagt tgggctcaaa taggtatcca
    29881 tgaaaagcct attgccattt ataatattaa tggctttttc aatccactac aaacaatgat
    29941 tgaccatatg attgaggaag gttttattga tcctaagtat cgtgctcttg caccgttatg
    30001 cgacactaag gaatcattaa ttgaatcaat attaaatttc aaacctctgg gtactcgttc
    30061 atacgattaa tcattctaag gttaaaaaat aaacaaacac ccctaatatt gttatcatca
    30121 gctaaacaat gttaggggtg tttgttacgc ttatgtataa atttttcact ttcgaagatt
    30181 aatgtaatag cgcacttgcc tttttaataa atgatactta cttagattgg gtaattcgaa
    30241 atgatcatac actgtcatgc caattttttc cattacacgt tgtgaagctt tatttgcttc
    30301 tgctgtaaaa ctatagacat cgtatatttg atgttcttct gctaacttca atgttgcctt
    30361 tgcgccttca gttgctaatc cttttcccca aaattctggc aacaagcgcc aacctatttc
    30421 ataaagcggt aattctttaa atggataatc gcttgtttct ggaatataat tcaaacctat
    30481 aaagcctatc cattgatggg actctttatc ttctacagca aataatccaa tgccataatc
    30541 tttaataacc gcatccatag ttctcatatc taattctgaa cgacgataac tcaataaact
    30601 tggaaaatat ttacgtacgt cataattcgc attcattttt tgaaaaggta acagatcatc
    30661 ttcatgccag tctcttaata ttaaacgctc tgtttcacaa taaatcatcg attgacactc
    30721 ctatataatt tctcaataaa aaacacaaaa tcgatatttt gatcaacttt gcgttttaga
    30781 tagcagttta ttttgattta gaaatataac ttataagttc aacatgaact ggcacttctt
    30841 ttaaacgtgc tctatctgat ttcagcgcag taaaagttgc atctataaaa tcttgttgat
    30901 ggtttaaacg atatgtagca acaaaagtat ctgtatcgga ctcaaagttt ggtaaatgtg
    30961 caattacacg cttaatttcg ccagtagaat cagcaatcgt atgtcctgta atattttcta
    31021 attctctacc taattctggc aaagtaatat cttgaccctc taaaattcta aaatcttgtt
    31081 catgcatgcc gtttctctcc tttattaatt acgttgtcta tttttacgtt ctgtttcaat
    31141 catgaccaca ttttaattaa tacataccct taaaataagg tttaaaacgc tgtacctttc
    31201 atctataaca agtaagcaat tcgattatga ttggtgtttg tcattagctt tttgatcttt
    31261 atctgctgat gtaggctttt tgtgattatc tttatccgat gacttagttt gatcttttga
    31321 ttgcttatct tgtgatgcct ctttatcttt atctttgtct ttatttttgt cttcgtcttt
    31381 tttatcatcc ttgttatctt cttttgaagg ttttaaatct ttcacttctt tttcaaggtc
    31441 tttttgctta tctgctaatt tttctttttc ttgttgaagc ttttcttttt tgtcttttaa
    31501 ttctttattt tctttttgta aattatcgat atccttttcc agagtggctt tctcttgact
    31561 tttaccacaa cctgttaaaa atagcatgac cgccataata ctgatgatta atttcttcat
    31621 ataaaactcc ttaacgtcat taaatctttt ttaattttaa catgaattag caacataatc
    31681 ctaattctaa tcaatatctt tatacatttt cacatgactc acttgataac ctaaatcttt
    31741 attcaaagat atcattggca aattattttt atgaattgta ttgcttattc gctttgcatt
    31801 catagttttt gcccattttt caagcgcaat cttcagttgc gtagctattc ccagtttgcg
    31861 aaattgtggt tcaacatata gcagttcaat gttaaccata cttttttcat tactaaaatg
    31921 tccccaaata aacgctatta attgaccttc attttcatat atataaattt tatcatttgt
    31981 atgttctaat cttgaacata tcatctcgta cctaagagca atactcagtt tagtacattt
    32041 atagttggat tctacttgcg acaataatgt ctcatgtata ttagctattt gcttgatata
    32101 attatgttca tctttattaa gtgtcctcat tttcctctcc aatattaatc gttatattta
    32161 tgcgtttaat atgatatgat tgaccttatt aatactataa aatcatttcc attttaattt
    32221 ggaattaaac atttttaaag gagcgcttaa gtaacaatga aaagtactgc gcaattaaca
    32281 aaagaaaata atgttaaatc attaagatta agcaatactg atagagaaat cttcgagaat
    32341 tatatgactt atatgcgctc tgactttcgc gtcaaccctc atgatacaga gttaattatt
    32401 aaccgtatac tcaaacaatt attaagcgca gaacaacatg gtttattagc cttagatttt
    32461 ttcaatcacg acccaaaagc acatgcaata aaagaactaa aagcgatgcc taatgaaaca
    32521 tttaaaaata tttttaaata tatctatcaa cacatcgttc tattaattgg gattgttagt
    32581 tttctcaaag gatttttagg atttttcatg gaaaaaaatg gaagtaattt atattttgtt
    32641 tcattcccgt tttcagtagt cgttggattc tttattgtct ttttatttat atggtttagt
    32701 tttaaaacaa tacaactgca gtgttttaac aattcaaatt ggatatggat attcacctat
    32761 ttagcaatca ttctactaat tgtaggattt ttctatgtat tctttattcc acaatcatta
    32821 cttgcctttg gaccatatat tcaggtaagc aattgggtat ttattatatt ttcatt
//
LOCUS       DANRDI010000014        31807 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.014, whole genome shotgun sequence.
ACCESSION   DANRDI010000014 DANRDI010000000
VERSION     DANRDI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.014"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..417)
                     /locus_tag="PZ377_000477"
     CDS             complement(<1..417)
                     /locus_tag="PZ377_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDM6357368.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWML"
     gene            771..1493
                     /locus_tag="PZ377_000478"
     CDS             771..1493
                     /locus_tag="PZ377_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDM6357369.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            complement(1679..1954)
                     /locus_tag="PZ377_000479"
     CDS             complement(1679..1954)
                     /locus_tag="PZ377_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357370.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(2109..3173)
                     /locus_tag="PZ377_000480"
     CDS             complement(2109..3173)
                     /locus_tag="PZ377_000480"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDM6357371.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            3391..4257
                     /locus_tag="PZ377_000481"
     CDS             3391..4257
                     /locus_tag="PZ377_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDM6357372.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            4599..4877
                     /locus_tag="PZ377_000482"
     CDS             4599..4877
                     /locus_tag="PZ377_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDM6357373.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            4892..5260
                     /locus_tag="PZ377_000483"
     CDS             4892..5260
                     /locus_tag="PZ377_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357374.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            5248..5544
                     /locus_tag="PZ377_000484"
     CDS             5248..5544
                     /locus_tag="PZ377_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDM6357375.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            5878..6984
                     /locus_tag="PZ377_000485"
     CDS             5878..6984
                     /locus_tag="PZ377_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDM6357376.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            6978..8318
                     /locus_tag="PZ377_000486"
     CDS             6978..8318
                     /locus_tag="PZ377_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDM6357377.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            8416..9306
                     /locus_tag="PZ377_000487"
     CDS             8416..9306
                     /locus_tag="PZ377_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDM6357378.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(9453..10283)
                     /locus_tag="PZ377_000488"
     CDS             complement(9453..10283)
                     /locus_tag="PZ377_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6357379.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(10303..11313)
                     /locus_tag="PZ377_000489"
     CDS             complement(10303..11313)
                     /locus_tag="PZ377_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDM6357380.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(11411..12115)
                     /locus_tag="PZ377_000490"
     CDS             complement(11411..12115)
                     /locus_tag="PZ377_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDM6357381.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(12108..12665)
                     /locus_tag="PZ377_000491"
     CDS             complement(12108..12665)
                     /locus_tag="PZ377_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDM6357382.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            12796..13095
                     /locus_tag="PZ377_000492"
     CDS             12796..13095
                     /locus_tag="PZ377_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDM6357383.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            13115..13936
                     /locus_tag="PZ377_000493"
     CDS             13115..13936
                     /locus_tag="PZ377_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDM6357384.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            13953..14330
                     /locus_tag="PZ377_000494"
     CDS             13953..14330
                     /locus_tag="PZ377_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6357385.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(14480..14710)
                     /locus_tag="PZ377_000495"
     CDS             complement(14480..14710)
                     /locus_tag="PZ377_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDM6357386.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(14930..15430)
                     /locus_tag="PZ377_000496"
     CDS             complement(14930..15430)
                     /locus_tag="PZ377_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357387.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(15628..16197)
                     /locus_tag="PZ377_000497"
     CDS             complement(15628..16197)
                     /locus_tag="PZ377_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDM6357388.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            16352..16480
                     /locus_tag="PZ377_000498"
     CDS             16352..16480
                     /locus_tag="PZ377_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357389.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            16607..17662
                     /locus_tag="PZ377_000499"
     CDS             16607..17662
                     /locus_tag="PZ377_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDM6357390.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            18270..18971
                     /gene="isaA"
                     /locus_tag="PZ377_000500"
     CDS             18270..18971
                     /gene="isaA"
                     /locus_tag="PZ377_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDM6357391.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            19247..19735
                     /locus_tag="PZ377_000501"
     CDS             19247..19735
                     /locus_tag="PZ377_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357392.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
     gene            20246..22057
                     /locus_tag="PZ377_000502"
     CDS             20246..22057
                     /locus_tag="PZ377_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDM6357393.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            22418..23185
                     /locus_tag="PZ377_000503"
     CDS             22418..23185
                     /locus_tag="PZ377_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDM6357394.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            23360..23848
                     /gene="crtO"
                     /locus_tag="PZ377_000504"
     CDS             23360..23848
                     /gene="crtO"
                     /locus_tag="PZ377_000504"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDM6357395.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            23841..25334
                     /locus_tag="PZ377_000505"
     CDS             23841..25334
                     /locus_tag="PZ377_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDM6357396.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            25340..26467
                     /gene="crtQ"
                     /locus_tag="PZ377_000506"
     CDS             25340..26467
                     /gene="crtQ"
                     /locus_tag="PZ377_000506"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDM6357397.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            26504..27367
                     /locus_tag="PZ377_000507"
     CDS             26504..27367
                     /locus_tag="PZ377_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDM6357398.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            27379..28887
                     /locus_tag="PZ377_000508"
     CDS             27379..28887
                     /locus_tag="PZ377_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDM6357399.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            29314..30468
                     /locus_tag="PZ377_000509"
     CDS             29314..30468
                     /locus_tag="PZ377_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDM6357400.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            30491..31489
                     /locus_tag="PZ377_000510"
     CDS             30491..31489
                     /locus_tag="PZ377_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDM6357401.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(31579..31785)
                     /gene="copZ"
                     /locus_tag="PZ377_000511"
     CDS             complement(31579..31785)
                     /gene="copZ"
                     /locus_tag="PZ377_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDM6357402.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
BASE COUNT        10798 a         4788 c         5914 g        10307 t
ORIGIN      
        1 taacatccaa cgaacaccgt atttatcggt aaagacgccc attttgccac cccagaattg
       61 gtcagcaaac ggtaattcta tttcaattga agaatgatct ttaatttgct catagaatgc
      121 ttcaacttta tcagcatctt ccttattgtt aacatcataa tcaattaata atgatatgcc
      181 attattaatt ttgtcagcgc gaccaaaaga atcagaacat aacactttta cgccaagcac
      241 ttcaaattca gcatgcatag ttgcttcttg cgcttcttcc ttagtcatac caaaatgtga
      301 cgcttgttct tcgccaactt ctaaacgttt aacgtcagtt gcaccaaata cttcttcgta
      361 atatgcaagg gcttcttttg aattttcaaa agcgatataa gggaataatg ctgtcatgta
      421 aaacacctca atgtattatt tttattacta acaccatgtt acattaaaaa atggcaaaag
      481 tataactttt acgatgaatt gaagtcaatc ttttgtaaaa tacacgataa attataagat
      541 gaaattgatt taaatgaagt aaacatatat atgacataca aaaaatgaaa atgacacaga
      601 gacgtatgtg ttgtttagtg ttttaaaatt caagtttaaa aaatgctttt agatacataa
      661 gggattttcg aaatataaat gcattaaaat gaatttctaa cttttttgac tgacatttaa
      721 tatgaatcat atataataga agttaatgat taaatgtggg aggttaaatc atgattaatg
      781 ctgaacctat tatcagtaaa atgaaaaatc aaaaaattaa ttatgacaag gtgttgaaga
      841 aattaatcgg gcagtgggaa agagaagcaa tcagacctaa aatcttattg catagttgtt
      901 gtgcaccttg tagtacatat acattagagt ttttaacaca atatgcagac attgcaattt
      961 atttcgcgaa ttcaaatatt catccgaaaa atgagtactt acgacgtgct aaagtgcaag
     1021 aacaatttgt ggaagatttt aatcgcaaga ctggtgcgaa tgtgaagtat attgaagcac
     1081 cttatgaacc gcataagttt gtgaaaatgg tgaaggataa agaattagct gatgaaaaag
     1141 aaggcggctt acgttgtacc gcttgttttg aaatgcgttt ggatattgta gcgaaagcag
     1201 ctgtagaaca tggctatgat tattttggca gtgcaatcac attatcacct aaaaagaacg
     1261 cacaattaat caatgaactt ggtatggatg tccaaaaaat atacgatgtg aactatttgc
     1321 caagtgattt taagaaaagt aaaggttatg agcgttccat agaaatgtgt aatgactata
     1381 atatctttag gcagtgttat tgtggatgtg tctttgcagc gatgcaacaa ggtattgatt
     1441 ttaaaacagt taataaagaa gcgaaagcat ttttagaaca atatccagat taatcattgg
     1501 aaatgagata aaaggcaaga acgtatacat gccatatccc attaaaaatt atcaaataga
     1561 aacccgagat tttagtgaaa tgaatcatac taaaatctcg ggttatttat ataatcgtaa
     1621 atattgtaga tataatgaat aactggatcc tgagacataa aacaatgtct cactctaatt
     1681 acatcatttt atgaacaaat cttataattg ctaaaatgac aatgacaagt gttgaaataa
     1741 gtgaaaacac tttaaacttt ttatagtcat ttttcgtttc attttctcct gttttcatca
     1801 tttcacgtag ctcttgctcg atttctaaat caagatctgt tttacgataa ccattcaaac
     1861 cttgacgttc atcttcttca ggttgatttt gttgctgtgt gtctttgttg tcagaagtcg
     1921 ctactgtttt tttattatct gtttctttag tcataacaaa cgcctccgtt ataaaacgct
     1981 atatttaatg atatgtgata tttaagccat tattttgaaa aatggtgata aatatatctt
     2041 cttgagttat atcataattt taataagacg attcagcaag ttttaaagta ttatttgact
     2101 atgttggatt aggcatctag tcctataata tcactgacat tgtcaaaatg atgatctttt
     2161 aagtaacgtg cgatgccttt gttcattttc ttagttaaac ctgggccttc aataacaagt
     2221 gatgaataaa tttgaataag tgacgcaccg tgacgcatca ttttgattgc atcttcagta
     2281 ctgaatacgc cgcctgtacc tataattaaa aattcaccat ttgtttgctg ataagcatac
     2341 ttaatcaatt ttaaattacg ttcaaataat ggacgaccac tcaaaccgcc ttcttcgact
     2401 ttattagcag aagttaaacc atctcgttgt cgcgttgtgt ttgctaagat gataccgtca
     2461 aatgtctcag taatcgctgg taatagtgct tttaagccat cgaaatccat atcagacgtt
     2521 agttttaaat aaattggcac tgttacatca tgttgttttt taaatgctgt taaagcttgg
     2581 cataacattg aaaattcatc tttatcatgg aagttttgaa gattttcagt atttggagaa
     2641 ctgatgttga ctgtgaaaaa tgaaacgtcg tgtttaaacg tatcaataac ctttatataa
     2701 tcttgataac gcgcttcata aggtgtcatt ttattcacac caacattgat accaacaggt
     2761 acttgataag catttttacg caaatgactt agtgctttgt tcataccaat attattgaag
     2821 cccattcgat ttatcaaggc gtcatcttct aataatctaa acatgcgtgg ttgagggtta
     2881 cccggttgag gtttaggtgt gataccacct aattctaaag caccgaatcc aaggtgttcc
     2941 aatgcttttg gtacttcgca agatttgtcg aaaccagctg ctaagccaat tggattgtcg
     3001 tacgtattac cttgtatcgt ttgtgataac gttggattct tataagtaaa tagtttatcg
     3061 acgactggga ataaaaccgg aaacttttgt aacgttttta atgcatcgat agttagtccg
     3121 tgtgcttttt cgggttcgat tttgaataag aaaggtttaa ttaatttgta catgagtatg
     3181 ctcctatttc attatatttg aggcttacta tcctcaactt aatatatgtg aaatatattc
     3241 ttttaataga ctagcatttc catacataat ttcctagtta aaactaaaaa gttttgaaaa
     3301 ttgacgcaag tttgaataac gtttttaaga ttaaatcatc ctaattaggc aatattatag
     3361 tataaagtaa gtagattgga aggtgtttgt atgaatgaac aatggttaga gcatttacct
     3421 ttaaaagata ttaaagagat ttcaccagtg agtggtggtg atgtaaacga agcatatcga
     3481 gtcgaaacag atacggatac atttttctta cttgtccaac gtggacgtaa agaatcattt
     3541 tatgctgcag aaattgcagg tttaaatgaa tttgaacgtg caggtatcac ggcacctaga
     3601 gtaattgcaa gtggcgaggt taacggtgat gcgtatttag tgatgacgta tttagaagaa
     3661 ggggcttcag ggagtcaacg ccaattaggg caactcgtag ctcaattaca cagtcagcaa
     3721 caagaagaag gcaaatttgg cttctcatta ccttatgaag gtggcgatat ttcttttgat
     3781 aatcattggc aagacgattg gtgtaccatt tttgtcgaca aacgcttgga ccatttgaaa
     3841 gatgagttgc taaatagagg gctatgggat gccaacgata tcaaagtata tgacaaagtg
     3901 cgacgtcaaa ttgtggcgga attagaaaag catcaaagta aaccgtcttt attacatggt
     3961 gacctatggg gtggtaatta tatgttctta caagatggtc gtccggcgtt atttgatcca
     4021 gcgccattat atggtgacag agaattcgat atcggtatta caacggtatt tggtggtttt
     4081 acgagcgaat tttatgatgc gtataataaa cattatccac tcgcaaaagg tgcatcctat
     4141 agacttgaat tttatcgttt atatttattg atggtccatt tattgaaatt tggtgagatg
     4201 taccgtgata gtgttgcgca ttctatggat aagattttac aagatacaac aagttagtta
     4261 agacgttaga ttgagataaa tagataatat gcacagatat ttttacaatg agaagcgata
     4321 cagctgcctc aataaaaata tttgtgcgtt tttattgttg gaaaataaaa ttttaatcgc
     4381 tattgttaat ttctgtaatg taaaacaagg ttgagttaca ataaaagtga ttttataact
     4441 ttttgttcaa taaaattcta ggaatgatac atatttattg atacaataat tttgaatata
     4501 atcataaaac aatatttaag tataattgaa tgtttgaata tcatatattg atacagtttc
     4561 taataatttt aaaataattt aaatggagag aggtgtaaat gatgagtaca gttcaaagtg
     4621 atatttttaa gaccaatagt gcatcatcat ctattaaaag cgctgttgaa acatgtaata
     4681 atgtgtcgaa accggataaa gatgaaagta caacagtaag tggaaataat aatgctcata
     4741 gtgtgataga tgatttgatg agtaagaatc aatctgttgc tgaagcaata cgaactgcga
     4801 gcgataatat acaaaaagtt ggtgaggctt ttgaccaaac tgacgtaatg attggtaatg
     4861 aaattggtaa aaattaaaac gtggtgaaat gatgtcgaat aaactggatg aaatcaataa
     4921 aataatcaca gcgaaacatg agcaaatgga tgacttatat gatgaaaagc gagaggttaa
     4981 agcattgata gatgaaagtg atgcgcttaa tcattcgata gatcaattat atcaacattt
     5041 aggtgagcgt tattatagta gcaatatggc tagtcgtatg gaacagttcc gcgatgaatt
     5101 tcattttgcg aaacgacgtt caacggaagc gttatacgag cagcaacagc aaattcaaca
     5161 tggcattcgt aaagtggaag aagagatgat tgacttggaa atgcgaagga atgttgaaat
     5221 tgagacggtg acaaaggagg aaaataaatg gaaacaatag gaagcattat ttatttaaaa
     5281 gaaggttcgc aaaagttaat gattattaat agaggaccaa ttgtagaaat tgaaaatcaa
     5341 aagtatatgt ttgactattc tgcatgtaaa tatccgattg gtgttgtaga agatgaaatt
     5401 tattatttta acgaggaaaa tatagattca gttattttta aaggttattc tgatcaagat
     5461 gaggttagat ttcaagagtt gtttgaaaat atgaaacaaa atttggatag tgaaatacaa
     5521 cgtggagaag ttacacaaca ataaagaaat actttttctt tattggggtg ggacgacgaa
     5581 ataaattttg taaaaatatg atttctgtct caccgccaaa atgttatctc caagtactga
     5641 ggctggatga taccatgaaa tgtcattcat aatcatttga actaaacgta gcagccttaa
     5701 attttaaaaa aagacacata ccaacttccg aaatgtagat gaattctcta caataacgga
     5761 agtttttctt ttaatattga aatttctcaa ggataggtct atactttata aatcgtaatt
     5821 attacgattt ataatcaaaa acaataactt gaaatagatc attgagggag tgttaatatg
     5881 caacatcata aagtggctat tatcggtgcc ggtgctgcag gtataggtat ggccattacc
     5941 ttaaaagatt tcggtataac agatgtcatt attttagaaa aaggaacagt aggacattca
     6001 tttaaacatt ggccgaaatc gacccgtacg atcacgccat catttacgtc taatggattt
     6061 ggcatgcctg atatgaatgc aatttccatg gatacttcac cagcatttac atttaatgaa
     6121 gaacatattt ccggagaaac atatgctgaa tatttacaag tggttgccaa ccattacgag
     6181 ctgaatatct ttgaaaatac agttgtcaca aatatatctg tagatgatgc atattatacg
     6241 attgcaacga caacagagat atatcacgcg gattatatct ttgtcgcaac aggtgattat
     6301 aatttcccta aaaagccatt taaatatggt attcattata gtgaaattga agactttgat
     6361 aactttaata aggggcaata tgtggttatc ggaggtaatg aaagtggctt tgatgctgca
     6421 tatcaacttg caaaaaatgg ctctgacatc gcactttata ctagcacaac cggtttaaat
     6481 gatccggatg ctgatcctag tgttagattg tcaccttata cacgtcagcg actaggtaat
     6541 gtcattaagc aaggtgctcg catcgaaatg aatgtacatt atacagttaa agatattgat
     6601 tttaacaatg gacagtatca tatcagtttt gatagcggac aaagtgtgct tacacctcat
     6661 gaaccaatac tagcaactgg ctttgatgca acaaaaaatc caatcgttca acaattattt
     6721 gtgacaacaa atcaagatat taaattaaca acacatgatg aatcgacacg ttatccgaat
     6781 atttttatga ttggtgcaac agttgaaaat gataatgcca aattatgcta tatctataaa
     6841 tttagagcgc gatttgcagt acttgcacat cttttaacac agcgggaagg cttaccagct
     6901 aaacaagatg tcattgaaaa ttatcaaaaa aatcaaatgt atttagatga ttattcatgt
     6961 tgtgaagtgt catgcacatg ttagaagtga aatatgatat gagaactggg cattatacgc
     7021 ccatacctaa tgaacctcat tatttggtta ttagtcatgc ggataaactt accgcaacag
     7081 aaaaagcgaa attaagatta ttaatcataa aacagaaatt agatatttca ttggcagaaa
     7141 gtgtagtttc ttcgcctata gcgagtgaac atgtgataga acaattgaca ctatttcaac
     7201 atgagcgacg acatttaaga cctaaaataa gtgcgacatt tttagcctgg ttgttgatat
     7261 ttttaatgtt tgcattgcca atcggtatcg cttatcaatt ttcagattgg tttcaaaatc
     7321 agtatgtgtc agcatggata gaatatttaa ctcaaacaac attgctcaat cacgatatat
     7381 tacagcatat attatttggt gattatggtg tgctatcact tggaacatat tcgctcgtat
     7441 gggcattgcc ggttgtaata ttgattagtt tatcaactgc tataattgat caaacaggac
     7501 tcaaatcatg gatgatatgg gcaattgaac cgtcaatgtt atggatagga ttacaaggta
     7561 atgatatcgt gccactatta gaagggtttg gatgtaatgc agcagctatt tcacaagcag
     7621 cacaccaatg ccatacctgc acgaagacac agtgtatgag tttaataagc tttggtagtt
     7681 cttgtagtta tcaaataggt gcgacattat ctatttttag tgtagctgga aagtcatggc
     7741 tatttatgcc gtacttaata ttagtacttt taggtggcat cttacataat aggatatggt
     7801 tgaaaaagaa tgatcaacaa cttagcgttc cgctacctta tgataggcaa ttacatatgc
     7861 caaatatacg tcaaatgttg ctacaaatgt ggcaaaatat acaaatgttt atcgttcaag
     7921 cgctacctat ttttatcaca atctgtctta ttgttagtat tttatcacta acgccaattt
     7981 tgaatgtttt atcacaaata tttacaccta tattatcgtt attaggcatc tcgtcagaat
     8041 tgtcaccagg gattttattt tcaatgattc gaaaagacgg catgctcttg tttaatttgc
     8101 atcagggcgc cttattacaa ggaatgacag caacacagtt actactactt gtgtttttta
     8161 gttcaacatt tacagcgtgc tcggtcacaa tgacgatgct tttgaaacat ttaggtggtc
     8221 agtcagcact aaaattaatt ggaaagcaaa tggtgacatc attgtcttta gttattggtg
     8281 taggcatcat tgttaaaata gtaatgctga ttatttaaaa aaaatgaact ataactgaat
     8341 atagagtcat gtcagtcaat aggagatcta tcttggaata tgctattcat atgaagtata
     8401 agaggagagt cgcagatgaa aatagttatt ataggtgggt ttttaggtgg cggtaaaacg
     8461 actgtcttaa atcatttgct cgctgaatca ttaaaggaat cgctgaaacc agcagtcatc
     8521 atgaatgaat ttgggaaaat gagtgttgat ggtgccttag tatctgaaga cataccttta
     8581 agtgaactga cagaggggtg tatctgttgt gcaatgaaag cagatgtatc agaacagtta
     8641 catcaattat atttaaaaga gcaaccagac attgtattta ttgaatgtag tgggattgca
     8701 gaaccggtct ctgtcttaga tgcttgttta acgcctattt tagctccgtt tacaacaatt
     8761 acacatatga ttggtgtaat agacgcaagc atgtataaac acattaaatc attccctaaa
     8821 gacatccaag gcttatttta tgagcaatta gcatattgtt ctgtcttatt tgttaataaa
     8881 atagattcag cagatgttga aacaacgagc aaactattga aagatttaga agttattaac
     8941 ccagaggccg atatacaagt cggtatgcat ggcagcgtca ctttgccaat atcagttaga
     9001 caaatgacag caacttctga caataaacat aagtctttac atcaaatgat taatcatcaa
     9061 tttgtgcaat caccagtcaa atgtactaaa gcagagttta taaaacgttt agcatgcctt
     9121 ccgtctcata tttataggtt gaaagggttt atgacatttg aagacaccgc acatacgtat
     9181 ctcattcaat ttacacaagg acaatatgaa ttaacacctg tagcattttc aaaaaaagtg
     9241 ccagaatatt tggtgcttat cggaaagggt atttcaaaag aagactatca atgtttggaa
     9301 cagtagtgtt ttcagtggaa gagaatggtt aacatgcctt catgtataat aacgagttga
     9361 tttgaacgtt taagcgtaaa taaaaataag cttggtcagc catcaaatat aatttgaaaa
     9421 ctgtccaagc tgttttatta gagaacaatc aattaacccc acatatttaa taatacatca
     9481 gcaaagcctt caggtttttg aatataacct aagtgaccgc ctggaatatc tacaataggt
     9541 atgccagttt ctttatttat ataaaagtta acatcttgtg ggaaggagcc tctagaatct
     9601 gtcccattta gtagggtgat tttatcgctg tattttgtga aatcatccaa agtaatattt
     9661 gaatgcgtat attgtctaat ttcaaattct aaccagaaca tcgtacgttt gtactgttct
     9721 atacgtcctt cttcagtatc agcaggttga gacatcattt ttgcatcaat tggtgcgata
     9781 tttaatgttt cgccaaatgt tttcatgcct ttttctaagc cttctgttaa aatttgatgc
     9841 acaatgtcat catttttatc tttccaataa gtactgtctg gtaaaaatgt attaattggt
     9901 ggttcgtgaa atgcaatctt tttaacgact tcagggtaat cttttaacac atgcatcgca
     9961 acgattgaac ctgaacttga acctaatata tagacaggtt catcacttaa tgactttgca
    10021 agttcggcaa tgtcctgtgc gtcgcgtttg acacgataat cactgtcagg gtttgaagcg
    10081 gaatcaggga gtggttcagt taactcgctt tctccataat cacgacgatc aacggctaca
    10141 acagtaaaat ggtcttttaa ctgttctgca agaggcagaa aaatgtctcc ggtaccgttt
    10201 gcaccaggaa taaagatgag cacgggtcct tgtccgactt ggtggtatcg taatttagcg
    10261 ccttgtaatt ctaaagtttc catattcaat gacctccatt tgttaattgt taggtgataa
    10321 acctaataat ttagcaccat ttgtataact tattttctct ttttcttcat ctgttaaacc
    10381 cagttcatct aaaaatacac ctaatttttc aggctcaata tatggataat cagcagcata
    10441 aagaattcta tcaataccta cttctttctt gactaaatca aactgtggct tcgttaacat
    10501 gccactcggt gtgatataaa aattattttt aaagtaatag cttacagggt ggttcaaatg
    10561 ttcagcgaat aaagcttcat ccatacgttc taagaagaat gggataaact caccccaatg
    10621 tccaataatc atatttaact ttggataacg atcaaaaata ccagataata ctagatgtat
    10681 tgcatgaatg ccgacatcaa tgtgccaacc ataaccaaaa caagcaaatg ttgccgcagt
    10741 tacttcagga taatttcctt tatagtatga ttgataaatg tcactgttaa ctggcgcggg
    10801 atgtagataa atcggtacgt ctaaattttc agctgttttg aaaataatgt catatttgtc
    10861 ttgatcaaga aaaccatctt gtgcacgtcc cataatgagc gcacctttga atcctaaatc
    10921 attgatgcaa cgttcgaatt ctcgcgctgc ggcttcaggc tcattgatag gtaaagttgc
    10981 aaagcctaca aagcgattgg gatattgtgc aatatagttt gccaattgat catttgcttt
    11041 ttgacataat tcaatggctt tctgaccaac caaatttgaa ggagaaccat ttccataaga
    11101 taagacttga atttgaacgt cttgattatt cataaattgg atacgttcat catgatgtga
    11161 taattcgtcg gcatttgtaa aacctgtctt tttttcaagg ccttctaaca ttactttcat
    11221 cggtacacct ttaggatctg ctgatatcgc attcatcgtt tctttttgaa tatcttcaat
    11281 gacataatgt tcttcaaacg taatactttt catttacttc gcctccatat tgtattgcat
    11341 gtttattgca tctattgcag aagcattttt tatatacctc taatttcaat gtttgtaaca
    11401 taaaattgat ctaccaaggc atctctccat cgccattaat aaatgtacct gttgggccat
    11461 ctgcaccaat cgttgctaat tgaatgattg gcttgattcc ttcagaaacg tgtttggaat
    11521 tattactaaa atcaccaact aaatcagtat ttgtagcgcc tggatcagca gcattgattt
    11581 gcatgttagg taatccttta gcgtattgta gcgttagcat tgttactgcc gatttagacg
    11641 aacaataagc taatgaattc actttagatt cagctgtttc ggggtttgta accattccaa
    11701 atgaacctaa accacttgat acgttgacga caacaggttg ttcagatttt tctaagagag
    11761 ggacgaatgt attcatcatt cgtacgatac cgaatacatt cgtttgatat acttcttcaa
    11821 cgtcacgagg tgtcaatttg gaaggtgctg aaaattgacc agatatacct gcattgttaa
    11881 tgaggatatc aagacggcct tctttttcag caatcatgtt ataagcattt ttgactgagt
    11941 agtcacttgt aacatctaat tgtacataat gaacacctaa tttttgtgat gcttgttgtc
    12001 ctcttacatc attccgagaa cctatataaa ctttgtaacc caatgcttta agtgcctctg
    12061 cacttgcata gcctaaccct ttattgcctc ctgtgattaa cacaatttta gtcattacgt
    12121 cccacctcat ctaaataaat gtttaataaa taatttctgt acgcttcaat tgaaatatgg
    12181 cgatgctcta tttggaaggc aaatacacta gttgataatg attgcaacag catatctgtt
    12241 ttgaacttcg tgtaagtgtc gtcatcgctt ttaaataagt cataataaaa atcaaataat
    12301 tcttgataaa atgcgctttg gtaaaaacgt aatttattgt tgcctgcttc aatacattgc
    12361 agtagtgcct tattatcgat tttaaattgt aaaagataat ctaacgacac ttgcataacc
    12421 tcataattag aatgatagtc atctttaatt tgcttaaaat gagtgataaa aatatcaagg
    12481 tctctttgta tgacgtagta gcataaatcg cttttatctt tgaaatgtcg atacaatgtc
    12541 cccataccga tacctagttc tttagcaata cgattcatac taatgttttc aacgccttct
    12601 tcatcaaaaa gtttgtgcgc tatttcttca attcgttgcc tattctcttt tgcatctttt
    12661 cgcatgatta cacctactta aaattctcta aaattgacaa acggataact ctccgtttat
    12721 tataaaacgt gttaagaaag ttagcaatga atttgcaata actattaaat atcataaaag
    12781 aaaagagtgt tgataatgtc tttaaataaa gagcaaagac gcatcacagc tgaagagttg
    12841 caagcacatt tcgaagcatc taccttatcg gttcaaatga ttgctgaaaa actgaatgtc
    12901 actacagaag atgtggaaaa agtattagct atgacagcgc cactaggcat ttttagtcat
    12961 caattacaac gatttattca tttagtatgg gatgtcagag atgtaataaa cgacaatatt
    13021 aaaggaaatg gacaaacacc agaaccatat acgtatttaa aaggtgaaaa agaggactat
    13081 tggtttttaa gataaaaaga ggagtggatc taaaatgaaa gatattttag taataggcgc
    13141 aacagggaag caaggtaatg cagttgtaaa acagttgtta gaggatggat ggtatgtaag
    13201 tgcattaact agaaataaga ataatcgtaa actttcagac ataggacatc cgcatttatc
    13261 aatcgttgaa ggtgatctga gtgataatgt tagtttgcaa tcagcgatga aaggtaagta
    13321 tggcttatac agtattcagc caatcgttaa agatgatgtt agcgaagaat taagacaagg
    13381 tatgaagata attgaaatag ctgagcaaga aaatattcaa catattgtgt atagtactgc
    13441 gggaggggtt aatcgaaatc gaactggccc acactttgaa gtactagcaa aaatagaaaa
    13501 tagattaatg gaatctaata taaatgcaac agttatcaaa ccatcattct ttatggataa
    13561 ttttttacgc attgctaaag tcgaagatga acgtattaca ttaccagaat ttattaatcc
    13621 gaatataaaa ttcacaatga tttcttctat tgatatagct aaaattgcat cgtatatttt
    13681 tgcacatcca caaagcttta cgcatcaatc aatagagatt ggttccgatg aagttacatt
    13741 aagtgaagca gcaacaattt ttagtgaagt gacaggtaaa tctactgtta tagaaggaga
    13801 atttgttagt ggtgttgcag aaaagcaatg gttggaagaa aaaggttatg aagtagactt
    13861 tgaattaatg gctgaaataa atccaacaag attatcatta agtgattggc ttaaagtcca
    13921 aaactataat aagtaatgaa tggggtgcaa tcatgattca atcaatgtgg tttaatttgc
    13981 atgtgcaaga tttagaaaag agcgcacagt tttataaagc gttaggattt aaaataaata
    14041 gaaacccaca aatgttagat aaaatggtcg gcattcaaat aggtcaaaca accgtaattt
    14101 taatagaaaa caagcatttt caaaatgtaa gtcagcaaag ccttaatact gaaccaaatg
    14161 aagtgatgat ttctctaggt gtgaacacaa atgaagaagt tgaccagttg gtgaataaag
    14221 tgaaagaagc gggtggcaca gtcgttcaag aaccaacagt aagccaaggg ttttatggag
    14281 caatgttcaa agatcttgat ggacaccatt ttaatttttt ggtctgctaa aatgaaattc
    14341 tcaaaagaat aaataaaaag cccatacaga gatgaagcgt aatgtgcacc atggtgcgac
    14401 acattagtgt cttcaatcag tatgggcttt gtttatgagg tcagttggca ccttaagtgc
    14461 aatacctcgt gagcaaatat taattttgaa tataaccgta ttgttggcga agatcataaa
    14521 ctttatctgg tgtgacatcc tgaccgattg gatcaaccac ttttattgtg gaaaatgttg
    14581 ttaatacttg tcctcggatc atgcttaagt agtcttgacg caatgcatgt tgttcttgtt
    14641 tttcagctac agtaagtggt tgtacttttt ctttatttgc aagttcatta attctatcta
    14701 aaattttcat attgtcatat cctccttaga atcaagtgtt gatatatttt gagattattt
    14761 ttatatggta atttatcaac acattaagta atgtttttag tttctcggtc ttttgaccag
    14821 cagatttttg gaattaaaat aagggatttg agacacgatt ttactagtag tacgatggca
    14881 aagtgatgtt attgaagtgt cgtttgatat acatgtctgc aatgttgttt tataagacag
    14941 gatgacgtaa ctgagcaaag aaatgagcat tgtcgaatga catcgtgtgt cttattaaaa
    15001 ttgcaatggg catagcatag gtatattcac agtcgatttt cttgatttct acattatcat
    15061 aatacgtagt gtaaaagtta tacgacagtc gatagtcgat tcgcttttta ccacgagcaa
    15121 ttattatgtg ttgcttcaac gtcgacttta atttatttgt aatgtatgtc actacaccca
    15181 gactactctg attaatattt aatatattat gcgtgcgttc aaacccctga ggttcatctg
    15241 tctcgctcac gtcaatccct cctatcttag tatttaaaca acgcatatca actatgcaac
    15301 atatgttgtt tagacatact atactactat gtcgtacgtt tgccaataaa cacgcacata
    15361 tcatttgttg cctaaacaac atattgcctg aagtgttgtt taagtacgca aaaacaaaaa
    15421 tgcttgtcac taaaatctat atgttttaat gacaagctta ttttcattca gttaacgact
    15481 gctaatatga cattgtagag tataggacgt tgatttatgt cctagacacg gctaatcaat
    15541 cgtaatacgt taaattttat tgtgtgtaca agtatgaacc taagtcacat agtgatttca
    15601 agatgcatga ctaaatacca agttttatta aaaatgtgct atatgatgaa ttaaaaatgg
    15661 aaggttgcga tcgataaaca catcggctgt ctcttgactc gtgttaaacc aatgtaatgc
    15721 taagcctgag ataccagctg ctaaaaaact cgcactattt acaatgatat ctcgttgatg
    15781 ctccggatag ctgttttcta acataatcgc aaaaatatct tctaattcat tattaatacg
    15841 ttgatgtgct ttgttgcaaa atgcttcact atttaattcg caagactttg cagcatcttt
    15901 gatataactc gcaattgaaa tgaaaatatt acgcagaacc ttttcattaa tgacattaga
    15961 aatgctcaaa ttatcattca agtcttttaa aatggttaca gataatgtgt agtctaggag
    16021 gtcttcttta tcagtgaaat gagcgtaaaa tgttgcacga tttagcgtag caatatcagt
    16081 gatatcttta actgtaattt gagataattt cttttctcta gaaattttaa gaaaagcatc
    16141 gacaagcaat tgtttggttc taacgattct tggatctatg gatttacttt ccatcatttg
    16201 ttcactacct aacatctgtt gttttaacac ttattatcag tatcttctat tatattgtca
    16261 attgtgttga tgatatgtaa gtgctgatac gctcctaaca aataatgtac ctttttggta
    16321 agcatttgaa tgtctttctt ctattgtatt aatggatttt aaaaatgaag ataaaataaa
    16381 agtgtattgt aacaatatgt ttgaaaaatt gccaatttct ttatctgaaa attcagaatt
    16441 ttgtgatata ctatttttca tagtcttgag ttatagatga tactgctagc ctaaatataa
    16501 caacatcggc atgcacatct tagaatttaa gctagtggca gcttataact taaggctctt
    16561 tattatttgc atacagatgt atatacataa atggaaggat gatattatga catcaattaa
    16621 aacaattaca cccaaagatt ttatatttag agttttatca ggtgtcgcca taggtattgt
    16681 tgcgggttta gttcctaatg cgatacttgg agaaattttt aaatacttta tggattatca
    16741 tccgattttt aaaacgttat taggcgttgt cgttgcaatt caatttacag tacctgcttt
    16801 gattggtgca ttggttgcca tgaaatttga cctatcgcca ttagcaattg ctgttgtggc
    16861 aagtgcagca tatgtaggaa gcggtgcagc gcagtttaaa aatggtgctt ggatgattac
    16921 gggtattggt gatttaatta atacaatgat tacagcagcg attgcagtgt tattcatttt
    16981 acttattcaa catcgcgtag gtagtatggc attgattgta ttcccaacag ttgttggcgg
    17041 tatttcaggt gcaattggtg ttttaatatt gccgtatacg aaaatgatta caacagccat
    17101 cggtaatatg gttaatggtt ttacagaatt acaaccgatt gtcatgagta ttttgatttc
    17161 aatggtattt agtcttatta ttatttcgcc actatcaact gtggccattg cctttgcgat
    17221 aggtattaca ggtttagcag ctggatcagc gtcaattggt atctccgcga ctgaagcagt
    17281 attgataatt ggtacaagta aagtgaatcg tttaggggtg ccgttatccg tcttctttgg
    17341 aggcgttaaa atgatgatac ctaatatggt gaaatatcca attcttatgt taccaatatt
    17401 aacgacagcc attgtatcag gtttggtatc agcattagta ggtattcatg gaacgaaaga
    17461 gtcagctgga tttggtttta taggaatggt aggaccgatt aatgcattta aatttatgga
    17521 ggttgattct gcttggttga gtgtcttact aattgtagta gcgttctttg ttgtaccatt
    17581 tgtaacagct tggcttgcag atatcattta tcgtaaagtg tttcgtttat atactaatga
    17641 tatttttaaa ttcatgggat aataacagta tgttttttga aaatatgaga ccaaaaatta
    17701 tttaatgtta ttgtgaattg cgagaatgaa gttgaacaat ataattgagc tgcagtttat
    17761 ttgcgctaat aaaagataag acaattcaca aaagatgtgt gttttaaaac tttgaatata
    17821 caacaataat agcaatcaat acatgtgtag aaacatattt gttgattgct ttttaattgc
    17881 gtttaatgta cgaaaataag agaagttaca tgtcagatat attacagcta tgtaacaaaa
    17941 atacaatctg taatattacg aaagctgtaa tagttgagct aaatatagta aaaatcttat
    18001 gtgttaaaga ctatttcaag ggttgattgg attctaaagg gcacatattt cacattacag
    18061 tataaaacag ccatatttca acattacaaa taacacttga tattgtaatg ttttgtaaag
    18121 aaagtgtaat ttactggctg gttttttgtg atatagtgag taccgttgaa agaaataaaa
    18181 gacaatttaa acttgcacta cgacattcaa attcaaaata taattactaa aaacataaac
    18241 ttacaattat tactggagga ttttttacta tgaaaaagac aattatggca tcatcattag
    18301 cagtggcatt aggtgtaaca ggttacgcag caggtacagg acatcaagca cacgctgctg
    18361 aagtaaacgt tgatcaagca cacttagttg acttagcgca taatcaccaa gatcaattaa
    18421 atgcagctcc aatcaaagat ggtgcatatg acatccactt tgtaaaagat ggtttccaat
    18481 ataacttcac ttcaaatggt actacatggt catggagcta tgaagcagct aatggtcaaa
    18541 ctgctggttt ctcaaacgtt gcaggtgcag actacactac ttcatacaac caaggttcaa
    18601 atgtacaatc agtaagctac aatgcacaat caagtaactc aaacgttgaa gctgtttcag
    18661 ctccaactta ccataactac agcacttcaa ctacttcaag ttcagtgaga ttaagcaatg
    18721 gtaatactgc aggtgctact ggttcatcag cagctcaaat catggctcaa cgtactggtg
    18781 tttcagcttc tacatgggct gcaatcatcg ctcgtgaatc aaatggtcaa gtaaatgctt
    18841 acaacccatc aggtgcttca ggtttattcc aaactatgcc aggttggggt ccaacaaaca
    18901 ctgttgacca acaaatcaac gcagctgtta aagcatacaa agcacaaggt ttaggtgctt
    18961 ggggattcta attccttcat aataaataaa agtaatgttt agacattaaa tatatagctc
    19021 ttcccataga aattggggag agttttttta ttaattaaaa atacattttt taaaaagtgg
    19081 atattgaagt tagggaaatt tattggactt tattgtatta ctgctaaaat acttgtcttt
    19141 attaagtgcc aagtggaatg aaacttcatt tgaggctgta ctgagtacac tagatacaaa
    19201 ctaaacgcac attttataat taaattaaat gaaaaaggag ataaaaatgg ctataaacat
    19261 aattgaatat aataggtctt ataaggaaga gctaatcgag tttattttat cgattcaaaa
    19321 aaatgaattt aatataaaaa ttgatagaga tgatcaacct gatttggaga acatcgaaca
    19381 taactatttg aataatggag ggcaattttg gcttgcaata aacaatcatc aaaatattgt
    19441 tggtactata ggtttgatta gactagataa taatatgtct gcacttaaaa aaatgtttgt
    19501 tgataaaggt tataggaatt taaaaatagg taaaaaactt ttagataaag ttataatgac
    19561 atgtaaagaa caaaatatcg atggtattta tttaggaaca attgataaat ttattagtgc
    19621 acagtatttt tattctaata atggcttccg tgaaattaag cgaggagatt taccaagtag
    19681 ttttcctaaa ttagatgtag ataataggtt ttattacaga aatttaaaag attaattcga
    19741 tgcaataata attgatatga caatcttagt aatattcatt ttaaaagtga cacatagcgt
    19801 ttaaatataa agtctgacga gatatatcgt ttggtttaca ttagaggata ggctgcattt
    19861 cgcttttttt tcaatacatg ttaatttatt cattatataa attttgaaaa agtatattcg
    19921 aatagcatat cttaatgatt ctgttacagt aatatgcata aaatataaaa cgaacctttt
    19981 ttgtaaaaat ataatgaaaa taacagttat tttgctaaaa attcaagaat ctatgataat
    20041 ctatgttatg tagtaaaatc gacaaattta tatgtggttt gcagtaatga aatcaactgt
    20101 aataattgtt ttctacttaa taaaaaattt aaaactatct taattaaggg ttaaatcaat
    20161 taatgaaaat cagtgcaata ttttattttc aaaaatattg tgatatgcta caaaatagag
    20221 aaaaataaat ggggcgttta ttaacatgga tacaaaagac tttaaacgtt tagaaaaaat
    20281 gtattccccg cgatacttac ctggattaga tggattgagg gcattcgcag ttataggaat
    20341 cattatttat cacttgaatg cacaatggtt aagtgggggc tttttaggag tagatacatt
    20401 cttcgttatt tcaggttatt taataacaag tttgttgata agtgagtatt atcggacgca
    20461 aaaaatcgat ttgctagagt tttggaagcg acgattgaaa cgactcattc cggcagtgtt
    20521 atttttaatc tgtgtcgtgc ttacgttcac actgatattt aaaccggaat tgattataca
    20581 aatgaaacga gatgctattg cagctatatt ctatgtttca aactggtggt acatttcaca
    20641 gaatgtagat tattttaacc aatttgctat tgaaccactc aaacatttat ggtctttagc
    20701 cattgaagaa caattttact tgcttttccc attggttatc acgttcttat tacatagatt
    20761 taaaccgaga aatattattc aaacgctatt tattgtatcg ttgatttctt taggacttat
    20821 gatagtgatt catttcatca ctggagataa ttcacgtgtg tattttggga cagatacacg
    20881 actgcaaact ttattgcttg gttgtatatt agcatttatt tggcctccgt ttgctttgaa
    20941 aaaagatatt tctaaaaaga ttgtcgtatc attagatatt atagggatat ctggttttgc
    21001 ggttctaatg actttgttct ttatagttgg agaccaagat caatggatct ataatggagg
    21061 attttacatt atatcatttg caactttatt cattattgca attgcggtac atccttctag
    21121 tttatttgct aaatttttaa gtatgaaacc tttactaatt ataggtaaac gatcatatag
    21181 cttatactta tggcattatc ctatcattgt ttttgtgaac agttattacg tacaaggaca
    21241 aataccggta tacgtttata ttatagaaat tttgttaaca gcgttaatgg ctgaaatttc
    21301 gtatcgcttt attgaaacac ctatacgtaa aaaaggattt aaagcttttg catttttacc
    21361 taaaaagaag gggcaatttg ctagaacagt gttagttatc ctattattgg ttccgtctat
    21421 cgttgtgctc agtggacagt ttgatgcact tggcaaacaa catgaagccg agaagaaaga
    21481 gaagaagacg gaatttaaaa caacgaagaa aaaagtcgtt aaaaaagata agcaagagga
    21541 taagcagaca gcgaatagca aagaggatat taaaaagtca tcaccactat taattggtga
    21601 ctcggtcatg gtggatattg gtaatgtctt tactaagaaa ataccaaatg cacaaattga
    21661 tggtaaagtt ggacggcaac tcgttgatgc tacaccaatt gtgaaatcgc aatataaaga
    21721 ctatgctaaa aaaggtcaaa aagttgtagt agagcttggt acaaatgggg catttacgaa
    21781 agatcaatta aatgaactat tggatagttt tggaaaagca gacatatatt tagtttctat
    21841 tagagtacct agagattatg aaggtagaat aaataaatta atttatgagg cagctgaaaa
    21901 gcgctctaat gtacatctag tcgattggta taaagcttct gcaggtcatc cggaatactt
    21961 tgcatatgac ggtattcact tagaatatgc aggtagtaaa gcgctgactg atttgattgt
    22021 aaaaacgatg gaaacacatg ctacaaataa gaaataattt gatgcactaa acttttgaaa
    22081 tattacatta cttctgatat ttattatcaa aaatgatgta tttcattaaa agtttagtgc
    22141 ttttttattt tcaaatccca tagtaacggt gcagaaaaag tgttgtaaac attctaattg
    22201 gtatattaca ttcaatgaag ctttattagg aacagattac attatgataa caaagcccgc
    22261 aagacaccta atctctgtta tagtttgttt tgtcgcaaaa ctataaaagt tataattgtt
    22321 tgcatactaa aaaaataaaa aatataaaat ttaaaataat tgagtcgcta atgactatat
    22381 gtatagagtg ttttgattat tgggaggata tttaattatg aaaaaaatcg ttacagctac
    22441 aatcgctaca gcaggacttg ccactatcgc atttgcagga catgatgcac aagccgcaga
    22501 acaaaataac aatggatata attctaatga cgctcaatca tacagctata cgtatacaat
    22561 tgatgcacaa ggtaattatc attacacttg gacaggaaat tggaatccaa gtcaattaac
    22621 gcaaaacaac acatactact acaacaacta caatacttat agttataaca atgcatctta
    22681 caataactac tataatcatt catatcaata caataactat acaaacaata gtcaaacagc
    22741 aacaaataac tattatactg gtggttcagg tgcaagttat agcacaacaa gtaataatgt
    22801 tcatgtgact acaactgcag cgccatcttc aaatggtcgt tcaatttcta atggttatgc
    22861 atcaggaagt aacttatata cttcaggaca atgtacttat tatgtatttg atcgtgttgg
    22921 tgggaaaatt ggttcaacat ggggtaacgc aagtaattgg gctaacgcag ctgcatcatc
    22981 tggctataca gtgaacaata caccaaaagt tggtgctatc atgcaaacaa cacaaggcta
    23041 ttacggtcat gttgcttacg ttgaaggcgt taacagcaac ggttctgttc gtgtttcaga
    23101 aatgaactat ggacatggtg ctggtgtggt tacgtctcgt acaatttcag caaaccaagc
    23161 aggttcatat aatttcattc attaatcaaa tgtaaatcaa atgacgtcaa tattctctaa
    23221 catgagagta ttggcgtttt tgttttatat aaatataaat gagagcggtt tattcactga
    23281 tctttaggga actaagtaat aaagtgataa tttatactat gtcagtatga ttgagagtga
    23341 ttcaatttag atgaaaacca tgaaaaaata tattaaaaca gcattttttt gtagtatgta
    23401 ttggttaatt gttcaactaa atatagcaaa tttaggtaca agaattcctg ataagtattt
    23461 tcgtcagaag tacataatat ttaaatcatt taactttgag aagcatggaa aattttggaa
    23521 caaatggttt tacgtaagaa aatggaaaca taagatttta gatggtcatc agcttaatca
    23581 aaatatatat gatcagcgtc atttaatgac aatcaatact gatgaaattg aaaaaatgat
    23641 tatagagaca aagagggcag agttgattca ttggatatcg atacttccag tcatcatatt
    23701 caataaaggc cctcgtttag taaagtatat aaatattttc tatgcaatga tagctaatgt
    23761 tccaatcatt attgtgcaac gctataatcg accgagatta acgcagttac tacgcatatt
    23821 aaaacgaaga ggtgaacgtc atgactaaac atatcatcgt tattggtggt ggcttaggtg
    23881 ggatttctgc agcaattcga atggcacaaa gtggctattc ggtctcatta tatgaacaaa
    23941 ataatcatat aggaggcaaa gtgaatcgtc atgaatcaga tggctttggc tttgatttag
    24001 gtccatctat tttaacgatg ccttatattt ttgaaaaatt attcgaatat agcaagaagc
    24061 aaatgtcaga ctacgttaca atcaagcgat tgccacatca atggcgtagc ttttttccag
    24121 atggaacgac tatcgatttg tatgaaggta ttaaagaaac aggtcagcat aatgcgatat
    24181 tgtcgaaaca ggatatagag gaactgcaaa attatttgaa ttatacaaga cgaatcgatc
    24241 gtattactga aaaagggtat ttcaactatg gtttagatac actatctcaa attattaaat
    24301 ttcatgggcc attaaatgct cttattaatt atgattatgt acatactatg caacaggcca
    24361 tagacaagcg tatctcgaat ccatacttgc gacaaatgtt aggctatttt atcaaatatg
    24421 taggttcttc atcatacgat gcgccagctg tattatctat gttattccat atgcaacaag
    24481 agcaaggcct ttggtatgta gaaggtggaa tccatcattt agccaatgcc ttggaaaagc
    24541 tagcgcgtga agaaggtgtc acaattcata caggtgcacg tgtggacaat attaaaacat
    24601 atcaaagacg tgtgacgggt gtcagattag atacaggtga gtttgtaaag gcagattata
    24661 ttatttcaaa tatggaagtc atacctactt ataaatattt aattcacctt gatactcaac
    24721 gattaaacaa attagagagg gaatttgagc cggcaagctc aggatatgtg atgcatttag
    24781 gtgttgcttg ccaatacccg caattagcac atcataattt cttttttacg gaaaatgctt
    24841 atctcaatta tcaacaagtt tttcatgaaa aggtattgcc agatgatccg accatttatc
    24901 tagtaaatac gaataaaact gatcacacac aagcgccagt aggttatgaa aatatcaaag
    24961 tcttaccaca tattccatat attcaagatc agccttttac cactgaagat tatgcgaagt
    25021 ttagggataa aattttggat aaattagaaa aaatgggact tactgattta agaaaacaca
    25081 ttatttatga agatgtttgg acaccggagg atattgaaaa aaattatcgt tctaatcgtg
    25141 gtgcaatata tggtgttgta gcagataaaa agaaaaacaa aggatttaaa tttcctaaag
    25201 aaagtcagta ttttgaaaac ttgtactttg taggtggatc agtaaatcct ggtggtggca
    25261 tgccaatggt tacattaagt gggcaacaag tcgcagacaa aataaacgcg cgagaagcga
    25321 agaataggaa gtgatatcta tgaaatggtt atcacgaata ttaacagtaa tagtgaccat
    25381 gtctatggcg tgtggtgcat tgatatttaa tcgtagacat cagctaaagg cgaaaacgct
    25441 gaacttcaat cataaagcat taacaattat tattccggct agaaacgaag aaaaaagaat
    25501 aggtcattta ctacattcga taatacaaca gcaagttcca gtagatgtca ttgttatgaa
    25561 tgacggatcg acagatgaaa cagcacgtgt agcacgttca tatggtgcga ctgtagtaga
    25621 tgttgttgat gatactgacg gcaaatggta tgggaaatca catgcttgtt atcaaggtgt
    25681 gacgcatgca tgtacgaatc gcattgcctt tgtagatgct gatgtaactt tcttaaggaa
    25741 agatgctgtt gaaacgttga ttaatcagta tcaattacaa ggtgaaaaag gattgttaag
    25801 cgtacagcct tatcatataa caaagcgttt ctacgaaggg ttttcagcga tatttaattt
    25861 aatgacagtc gttggtatga atgtattttc taccttagac gacggtcgga ctaaccagca
    25921 tgcatttgga ccggtgacat taacaaataa agaagattat tatgcaactg gaggtcataa
    25981 aagtgcaaac cgtcatatta ttgaaggatt tgctttagga agtgcatata cttcacaatc
    26041 attgcccgta acagtttatg aagggtttcc atttgttgca tttcgcatgt atcaagaagg
    26101 atttcagtca ttacaagaag gatggacaaa gcatttgtca actggggcag gtggcacaaa
    26161 gcctaagatc atgacagcaa ttgtgttgtg gttgtttggt tctatagcga gtattttagg
    26221 gctatgtctt agtttaaaat atcgccaaat gtctgtaaga aaaatggtag cactttactt
    26281 gagctatact acacaattta tttatctgca tcgaagggtc ggccaatttt ctaatttatt
    26341 aatggtatgt catccattgt tatttatgtt ttttactaaa attttcatcc aatcttggaa
    26401 acaaacgcat cgttatggtg tagttgaatg gaaaggtcgt caatattcta tatctaaaga
    26461 acaataaatc aaggtaatgg catttcaata taggaggact agtatgacaa tgatggatat
    26521 gaattttaaa tattgtcata aaatcatgaa gaaacattca aaaagctttt cttacgcttt
    26581 tgacttgtta ccagaagatc aaagaaaagc ggtttgggca atttatgctg tgtgtcgtaa
    26641 aattgatgac agtatagatg tttatggcga tattcaattt ttaaatcaaa taaaagaaga
    26701 tatacaatct attgaaaaat acccatatga acatcatcac tttcaaagtg atcgtagaat
    26761 catgatggcg cttcagcatg ttgcacaaca taaaaatatc gcctttcaat ctttttataa
    26821 tctcattgat actgtatata aagatcaaca ttttacaatg tttgaaacgg acgctgaatt
    26881 attcggatat tgttatggtg ttgctggtac agtaggtgaa gtattgacgc cgattttaag
    26941 tgatcatgaa acacatcaga catacgatgt cgcaagaaga cttggtgaat cgttgcaatt
    27001 gattaatata ttaagagatg tcggtgaaga ttttgacaat gaacggatat attttagtaa
    27061 gcaacgatta aagcaatatg aagttgatat tgctgaagtg taccaaaatg gtgttaataa
    27121 tcattatatt gacttatggg aatattatgc agctatcgca gaaaaagatt ttcaagatgt
    27181 tatggatcaa atcaaagtat ttagtattga agcacaacca atcatagaat tagcagcacg
    27241 tatatatatt gaaatactgg acgaagtgag acaggctaac tatacattac atgaacgtgt
    27301 ttttgtggat aagaggaaaa aggcaaagtt gtttcatgaa ataaatagta aatatcatag
    27361 aatataggtg gttgaataat gaagattgca gtaattggtg caggtgtcac aggattagca
    27421 gcggcagccc gtattgcttc tcaaggtcat gaagtgacga tatttgaaaa aaataataat
    27481 gtaggcgggc gtatgaatca attaaagaaa gacggcttta catttgatat gggtcccaca
    27541 attgtcatga tgccagatgt ttataaagat gtttttacag cgtgtggtaa aaattatgaa
    27601 gattatattg aattgagaca attacgttat atttacgatg tgtattttga ccacgatgat
    27661 cgtataacgg tgcctacaga tttagctgaa ttacagcaaa tgctagaaag tatagaacct
    27721 ggttcaacgc atggttttat gtccttttta acggatgttt ataaaaaata tgaaattgca
    27781 cgtcgctatt tcttagaaag aacgtatcgc aaaccgagtg acttttataa tatgacgtca
    27841 cttgtgcaag gtgctaagtt aaaaacgtta aatcatgcag atcagctaat tgaacattat
    27901 attgataacg aaaagataca aaagctttta gcgtttcaaa cgttatacat aggaattgat
    27961 ccaaaacgag gcccgtcact atattcaatt attcctatga ttgaaatgat gtttggtgtg
    28021 cattttatta aaggcggtat gtatggcatg gctcaagggc tagcgcaatt aaataaagac
    28081 ttaggcgtta atattgaact aaatgctgaa attgagcaaa ttattattga tcctaaattc
    28141 aaacgggccg atgcgataaa agtgaatggt gacataagaa aatttgataa aattttatgt
    28201 acggctgatt tccctagtgt tgcggaatca ttaatgccag attttgcacc tattaaaaag
    28261 tatccaccac ataaaattgc agacttagat tactcttgtt cagcattttt aatgtatatc
    28321 ggtatagata ttgatgtgac agatcaagtg agacttcata atgttatttt ttcagatgac
    28381 tttagaggca atattgaaga aatatttgag ggacgtttat catatgatcc ttctatttat
    28441 gtgtatgtac cagcggtcgc tgataaatca cttgcgccag aaggcaaaac tggtatttat
    28501 gtgctaatgc cgacgccgga acttaaaaca ggtagcggaa tcgattggtc agatgaagct
    28561 ttgacgcaac aaataaagga aattatttat cgtaaattag caacgattga agtatttgaa
    28621 gatataaaat cgcatattgt ttcagaaaca atctttacgc caaatgattt tgagcaaacg
    28681 tatcatgcga aatttggttc ggcattcggt ttaatgccaa ctttagcgca aagtaattat
    28741 tatcgtccac aaaatgtatc gcgagattat aaagatttat attttgcagg tgcaagtacg
    28801 catccaggtg caggcgttcc tattgtctta acgagtgcga aaataactgt agatgaaatg
    28861 attaaagata ttgagcgggg cgtataaggg agtagtctaa gagaaagatg tgagaaaagt
    28921 ataaggggaa cgtaaaaggc atagtgaaat aagtatgtgc ttaagaagtt ttatttaaca
    28981 atgaatgacg cctgttaagg ttcacgtttg aatacgtacc ttgatgggcg ttatacttta
    29041 tataagcata tctaactggt tgcgcatgcg gatatgcatg acagttaaat cataacgatt
    29101 gagtgcatat taatctgaaa attcctaata ttgacaataa tattatacct acctaaaatg
    29161 aaaataaatt cattcaatac aacgtgtgtc ataagttctg ttgaacataa cggtgaatgg
    29221 cgatgtcatt gtaaccatct aaatgagagt gatacaagtt gttaggatta tgatagtcgc
    29281 ccaggctatg tttaatgata gaggaggaat ttgatgatct ctaacaaatt agcaaatatc
    29341 cctgatagtt atttcgggaa aacgatggga cgtaaaatcg aacatggacc attgccatta
    29401 attaatatgg cagttggaat ccctgatggg cccacgccac aaggtattat tgatcatttt
    29461 caaaaagcac taactattcc tgaaaatcaa aaatatggtg cttttcatgg taaagaagcg
    29521 ttcaagcaag ctattgtaga tttttatcaa agacaatata atgtgacgtt agataaagaa
    29581 gatgaagtat gcattttata tggtacaaaa aatggattag tggcagtacc aacatgtgtt
    29641 atcaatccag gagactatgt attactacct gatccaggct acacggatta tttagcaggt
    29701 gtacttttag ctgatggcaa gccagtcccg cttaatttag aaccgccaca ttatttgcca
    29761 gattggtcca aagttgattc acaaataata gataaaacaa aactcattta tttaacgtat
    29821 ccaaataatc caactggatc gacagctacg aaagaagttt ttgatgaagc gatagctaaa
    29881 tttaaaggta cagacactaa aatcgtgcat gattttgctt atggtgcttt cggtttcgac
    29941 gctaaaaatc ctagtatact tgcttcggaa aatgggaaag atgttgcgat tgaaatttat
    30001 tccttgtcta aaggttataa catgtcaggt tttagagtcg gttttgcagt tggtaataaa
    30061 gatatgattc aagctttgaa aaagtaccaa acgcacacaa atgctgggat gtttggtgca
    30121 ttacaagatg ccgcaatata tgctttgaat cattatgatg attttttaga agaacaaagc
    30181 aatgtattta aaacacgtcg tgaccgattc gaagccatgc ttgcaaaagc tgatttgcca
    30241 tttgttcatg ctaaaggtgg catatatgta tggttggaaa caccgcctgg ttatgacagc
    30301 gagcaatttg agcaattttt agtgcaagag aagtcaatac ttgttgctcc agggaaacca
    30361 tttggagaga atggtaatcg ttatgtgaga atttcattgg cgttagatga ccagaaatta
    30421 gacgaagctg caataagatt aacagaacta gcatatttat atgaataata aagttaaggg
    30481 tgatgttaaa atgacaaaaa ttaaaatcat gagtgtacgt gatgaagata tgccttatat
    30541 caaggcgtgg gcagagaaac atcatgttga ggtagatatt actaaagaag cattaactga
    30601 tgacaatgtt gaaggtgtcg ctggttatga tggcttatcg ctatcacaac aaataccatt
    30661 atcagagcat gtttataaac gattaaatga actaggtatt aaacaaatcg cacaacgtag
    30721 tgctggattt gatacatatg atttggagtt agcaaacaag tataacttta ttgtgtcaaa
    30781 tgtgccgtca tactctccga attcaattgc ggagtttgct gtgaaccagg ctattaatgt
    30841 ggttcgtcat tttaatcaaa ttcaaacgaa agtgcgggag catgatttta gatgggaacc
    30901 tactatttta tcgaaatcca ttaaagattt aaaagtagct gttattggta caggtcgaat
    30961 tggccgtgta gtagccgata tatttgccaa tggttatcaa agtgatgtgg tcgcatacga
    31021 cccgtttcct aatgctaaaa ttgcaacgta tgtcgattac aaagatacga ttgaggaagc
    31081 ggttgaaggt gctgatatcg tgacattaca tgtacctgca acgaaatata atcattatct
    31141 atttaatgct gaattattta aacattttaa aaagggcgca gtatttgtca attgtgcgag
    31201 aggttcttta gtagatacta aggcgttatt agacgcatta gacaatggtg tgattaaagg
    31261 tgcagcactt gatacgtatg aatttgaacg caaacttttc ccaagtgatc aaaggggcaa
    31321 aacactgaac gatccattgt tagaatcgtt gattgacagg gaagatgtca tattaacacc
    31381 acatattgcg ttttatactg aagctgcagt taaaaatcta attgtcgatg cattagatgc
    31441 aacattagat gtattgcaga ctggagatac taggttacga gtaaattaaa aatcgaactg
    31501 atgagataat ttggattgtt gggattctca tcagttcgat tttttaattt ggtgttgatg
    31561 acgttgaata ttgcctaatt aaacgacatc gtaaccttga tcttcaattg cgtctttcat
    31621 ttgagataca gcaactttac tgtcatcata ttgaacactt acttgaccat tttcaaggtt
    31681 aacgtcagct gaagtgacac cgtcaatatt atttaatgca gattcaacag cacttttgca
    31741 gtgaccacag ctcatacctt caacatttaa aatttcttgt gacatagcat tcacctcctt
    31801 ttagagc
//
LOCUS       DANRDI010000015        31431 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.015, whole genome shotgun sequence.
ACCESSION   DANRDI010000015 DANRDI010000000
VERSION     DANRDI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.015"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1629)
                     /gene="vraE"
                     /locus_tag="PZ377_000512"
     CDS             complement(<1..1629)
                     /gene="vraE"
                     /locus_tag="PZ377_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HDM6357403.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRI"
     gene            complement(1619..2377)
                     /gene="vraD"
                     /locus_tag="PZ377_000513"
     CDS             complement(1619..2377)
                     /gene="vraD"
                     /locus_tag="PZ377_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDM6357404.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            2660..3172
                     /locus_tag="PZ377_000514"
     CDS             2660..3172
                     /locus_tag="PZ377_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDM6357405.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(3306..4100)
                     /locus_tag="PZ377_000515"
     CDS             complement(3306..4100)
                     /locus_tag="PZ377_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDM6357406.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(4343..5359)
                     /locus_tag="PZ377_000516"
     CDS             complement(4343..5359)
                     /locus_tag="PZ377_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDM6357407.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            complement(5524..6099)
                     /locus_tag="PZ377_000517"
     CDS             complement(5524..6099)
                     /locus_tag="PZ377_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDM6357408.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            6324..7232
                     /gene="rarD"
                     /locus_tag="PZ377_000518"
     CDS             6324..7232
                     /gene="rarD"
                     /locus_tag="PZ377_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDM6357409.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(7645..9063)
                     /locus_tag="PZ377_000519"
     CDS             complement(7645..9063)
                     /locus_tag="PZ377_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDM6357410.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(9301..9765)
                     /gene="bstA"
                     /locus_tag="PZ377_000520"
     CDS             complement(9301..9765)
                     /gene="bstA"
                     /locus_tag="PZ377_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDM6357411.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            9932..10270
                     /locus_tag="PZ377_000521"
     CDS             9932..10270
                     /locus_tag="PZ377_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDM6357412.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            10260..11027
                     /locus_tag="PZ377_000522"
     CDS             10260..11027
                     /locus_tag="PZ377_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357413.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(11146..11784)
                     /gene="pcp"
                     /locus_tag="PZ377_000523"
     CDS             complement(11146..11784)
                     /gene="pcp"
                     /locus_tag="PZ377_000523"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDM6357414.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(11936..12892)
                     /locus_tag="PZ377_000524"
     CDS             complement(11936..12892)
                     /locus_tag="PZ377_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDM6357415.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            13136..14107
                     /locus_tag="PZ377_000525"
     CDS             13136..14107
                     /locus_tag="PZ377_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDM6357416.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(14256..14771)
                     /locus_tag="PZ377_000526"
     CDS             complement(14256..14771)
                     /locus_tag="PZ377_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDM6357417.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            15151..15999
                     /locus_tag="PZ377_000527"
     CDS             15151..15999
                     /locus_tag="PZ377_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDM6357418.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            16028..16582
                     /locus_tag="PZ377_000528"
     CDS             16028..16582
                     /locus_tag="PZ377_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HDM6357419.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            16635..18347
                     /locus_tag="PZ377_000529"
     CDS             16635..18347
                     /locus_tag="PZ377_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6357420.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            18344..19177
                     /locus_tag="PZ377_000530"
     CDS             18344..19177
                     /locus_tag="PZ377_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDM6357421.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            19224..19721
                     /locus_tag="PZ377_000531"
     CDS             19224..19721
                     /locus_tag="PZ377_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357422.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            19839..20963
                     /locus_tag="PZ377_000532"
     CDS             19839..20963
                     /locus_tag="PZ377_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDM6357423.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            21268..22086
                     /locus_tag="PZ377_000533"
     CDS             21268..22086
                     /locus_tag="PZ377_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDM6357424.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            22104..22718
                     /locus_tag="PZ377_000534"
     CDS             22104..22718
                     /locus_tag="PZ377_000534"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDM6357425.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            22711..23961
                     /locus_tag="PZ377_000535"
     CDS             22711..23961
                     /locus_tag="PZ377_000535"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDM6357426.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            23977..24990
                     /locus_tag="PZ377_000536"
     CDS             23977..24990
                     /locus_tag="PZ377_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDM6357427.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            24959..25537
                     /gene="hisB"
                     /locus_tag="PZ377_000537"
     CDS             24959..25537
                     /gene="hisB"
                     /locus_tag="PZ377_000537"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDM6357428.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            25534..26112
                     /gene="hisH"
                     /locus_tag="PZ377_000538"
     CDS             25534..26112
                     /gene="hisH"
                     /locus_tag="PZ377_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDM6357429.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            26105..26809
                     /locus_tag="PZ377_000539"
     CDS             26105..26809
                     /locus_tag="PZ377_000539"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDM6357430.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            26806..27564
                     /gene="hisF"
                     /locus_tag="PZ377_000540"
     CDS             26806..27564
                     /gene="hisF"
                     /locus_tag="PZ377_000540"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDM6357431.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            27561..28193
                     /locus_tag="PZ377_000541"
     CDS             27561..28193
                     /locus_tag="PZ377_000541"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDM6357432.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            28386..28565
                     /locus_tag="PZ377_000542"
     CDS             28386..28565
                     /locus_tag="PZ377_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357433.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
     gene            29286..31328
                     /gene="lip1"
                     /locus_tag="PZ377_000543"
     CDS             29286..31328
                     /gene="lip1"
                     /locus_tag="PZ377_000543"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDM6357434.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT        11014 a         4748 c         5706 g         9963 t
ORIGIN      
        1 tattctgaag ttacttagtt catcctcagt ttcatccatt tgtttaatat aaataataca
       61 tccagctgct actaaaaatg ctaatcctaa aaatgatgta acaaatatta gaataccgtt
      121 agtagcatcg acctcttttt tcatttcatc atacgtgatg actttgtctc caaactgttt
      181 tgcaattgct tgagcttttt ccttttgtga tgtttgttta atatcatatc cataaaaagt
      241 atgaacgtta ttttgtgttt tcaactgctg atacttttca ggacttactt cgatgacagg
      301 tgagttgaag cttagattta aaggataaac cttacctttg tcttcttgtg taacacggaa
      361 agtttcattc ttagtacctt ttactactaa atctttgttt aaatggatat taataatgtt
      421 aggcagcgat tttgtatttg taatgatggc attgttgcct gttaacttgt tatttgcact
      481 taaaatagaa ttcgtgcgac ctgaatcact accattttcc aaagtaataa cctgatcatt
      541 aacattatct acagtaatag tttcgtaagc atttttagaa aatgtaattt gttgttggct
      601 tagtttagtt tcaaattgtt tagcatcttg agtagcgacc acgttaaatt catttggtgc
      661 catagatgta agggtttgat ctgtatttga tttagataat gccgcaaaac acaatacagt
      721 tactgtaact gcagaaataa ttgcaatgat agttaaagac atggcatttt tcttcattct
      781 gtacataata gacgatgtga atacaacatc ggtaatagat acgcgtccat tttttgattt
      841 tttcaatgtt ttaaaaataa gtgacacgga acttctgaag aataaatagg cgcctacaac
      901 cgttaaaaat aaaatgataa acggtgatgt catagccata gttagtgctt tgaacgtacc
      961 aaacatttct gtcgccatat aatagcctag tgcaatcata gcaataccta atacgcctga
     1021 aataacctct gcagtcgtta ctttagcagt tgtggcatca gttttaattg aatctttcat
     1081 cattgataag atactacgtc tttttagaaa taaagcactt ttaaataaaa tcagtacata
     1141 cgcaataatt agcatgaaaa tagttaaaac aagggccata ggttcgaaat gtatcgataa
     1201 gttaatcgat aacgacatca atttagatac tatggaaagc aataattgtg cacctgcaat
     1261 gccacataat acaccgacaa cacctgtgat taaaaatacg atcatttgtt caagtgctaa
     1321 cattttcaaa atgttttgtc gtgttaaacc aatcaactga aatagcgcaa attcacgtgt
     1381 acggcgtttt acgaataaat gattggcata cattaaaaag atgacaataa tgataaataa
     1441 aaatattgat ccgactaaag cacctttctt aatgatggcc atcgagtcgt cattatttac
     1501 acctttagta aactgtaagg ttgtaaaact gaaatataag acgatgctaa aaaataatga
     1561 aaataaatac attgcataat gttttaagtt ttgtcgtaag tttttgaaaa cgatatggtt
     1621 aaatgtcatt tgagacacca cctaatactg attgaagatg tacaatgtct tcataaaagg
     1681 cctgtttaga acgtccttcc tgataaagtt gtgtatgaat ttggccatct tttagcatga
     1741 tgacacgttc tgcaaaactt gctgcaaccg gatcatgtgt aaccatgaca atagttgtat
     1801 caaacgattt attcatttct tccaaacgtt gtaataggtc atttgcactt ttcgagtcga
     1861 gtgcgcctgt tggctcatct gcaaatatga tttgtggttt gtgaacaaat gctctcgctg
     1921 ctgcagttct ttgttgttga ccaccagata attcgctagg gtatttattt cctaggtcat
     1981 aaatacctaa tgctgtcgtg atcgctttat aattttcttc cattgttgcc ttcgacattt
     2041 tttgaacaga taaaggtaac ataatgtttt ctttaacggt taatgtcggc agaatactgt
     2101 aatcttggaa gatgaaacct aatgattctt tgcggaattt ggcaagtgct ttttgattaa
     2161 gtttattaag ctcttgtccg ttagcaatca cgctaccgct agaaatttgg tcaattgaac
     2221 ttagtacatt taataaggtt gtcttacctg atccagaagg ccccataatc gcaacgaatt
     2281 cgcctttttg tatgtcaaag ttaatatctt taagtgcttg aaatgtgtgc tttttaccgt
     2341 atgtttttga aacatgttgc actgataata tcgtcatagt ctcactcctt ttgtatttaa
     2401 tttcatttta aataatgttt ggagtagtag cctttatcta aacttacaat tcaatgaatg
     2461 aaccttacag agttgaaagc tatcgctact tagtagattt ttgagtgagg atacagattc
     2521 atcgtacata ttagacaaaa gcaatggtgc tttctaagtg atgatgtttg tgtaaattga
     2581 gaaaagggaa tttaattatt gtataataaa ttttttgtaa aaattaaaag agggttttat
     2641 ttgaaaggaa ttgattgtta tggaaaaagg aaatcaaggt attaaatggt ctagtttaat
     2701 aatgggtgta ttattattaa tgttggcagt cgttattttt acatttccaa ttgaaaattt
     2761 ttatgctatt acctggttga ttggactgtt tgtattaatt aacggtgtga ttcaaatcgt
     2821 ttaccgtaga aaagcaaaag ctttagtagg tggtaaccaa aattggattc tgtttatggg
     2881 gattgtagat attctatttg gtctattagt tatttttaat gttggcgcaa gttcagcatt
     2941 ctttatttat atgtttgctt tttggtttat ttttagttct atctctggat tatttacgtt
     3001 ttcgggtagt ggtagcttaa aactaatttc agtgattttt aatttattag gtattgtttt
     3061 cggtgtcatt ttattattta atccattaat gggtatcgtc tttatttcga cgatgattgc
     3121 tattgcattt gtattcgtag gtgtcattta tgttgtagat gcacttgctt aagtaaaatg
     3181 aagcggttca aaagaagggt gtgacatgaa gtttgtgtca tatccttttt gttgtgttta
     3241 tgaagcataa aaaaggggcg ctacctacaa taagtaagat acgcccatat ttttatattt
     3301 tactattatt gtttttcaat acgattaata gttacattta gtccaaaata tttttctaaa
     3361 aaatgtttat agttatcttt agtgacatca aatttttctg agctaccatt ccttgttaaa
     3421 gttaaatgat tttcagacat tgtagcacgg ccaaatgatt gtggcattgt aattaataaa
     3481 tgctgtacaa atattgaatc tggatgcgtt tgattatatt cgatgttgct attaaaatct
     3541 tctatttgtt taggtttgaa ttcagcttca tattttgtat gccaatgatc attttcgaat
     3601 ttttgaacat agaaaatatc cttgtcttcg ttgttaaaaa tagcgcggaa tgtaccactg
     3661 atatcagtaa ttggttgtgt atgctcagat gaagtaatag gaatggcatg tagaggtaag
     3721 tctccaaagc caacatcagt tacatagaat acatcattta tagaaacaac aagtgaagca
     3781 tgtgaaccgt tcagactacg accgccaccg ggtgtgtgaa tagtagctga cattaattca
     3841 ggattaaatc ctttttgttg taaataggct ttgaaaaatg tatttaattc ataacaaaaa
     3901 ccaccacgtt tatcatgaac aattttatta aaaagtgcat cgatatttat agatatcggc
     3961 ttactatttt gaacatcaat attttcaaaa ggtacagtta acataaaacg tgttgcataa
     4021 taatttaatg cttcaatact cggtcgatta taacgagatg aatcaatttg taaataattc
     4081 tctaacttcg caatattcat aagcatagcg cctcctgtat taaagattat aattaaattt
     4141 taaacagaaa tactgaaatt ttaaattcga aagcattgaa ttttggataa atacatttta
     4201 aatagaaaaa tacgctctca aaatgaagtc atctctaaaa gaaacgattt aaagatgact
     4261 actgagagcg tagcataatg gaagaagtgt gcagggtgtc taaaaatgca acaatacaaa
     4321 ggtagttgca agacaagttg ccttatctag accatttgtg ttctatgcga ccaaacttcc
     4381 aaattaaact tgaaataagc caagtaatta aaaataatgc aactaaaata tagcctaaat
     4441 aatcaaattc gatcgaacca atgaatgccc aaaacgcacc atgtaaatct aacttatcag
     4501 caagaatttg tagcaattca atcatcccaa tcactaatgc tgccatgact gatatcgcag
     4561 taatcgttat attgtaatag attttgcgaa taggattgaa gaatgcccaa ttataggcat
     4621 acttcattac aacaccatct aatgtatcca ataaactcat acctgatgcg aataaaattg
     4681 gtaaagataa gattccgata aatgaaatgg cttgttgtga tgcgcctgaa gaaagagcga
     4741 gtaacgcaat ttcactagct gtatcaaaac caagtccaaa taaaaagcca agtggcaata
     4801 cgtgccaact acgcgtgatt aatttgaaat aaggtcctac aaatcgagaa accaatcctc
     4861 tagattcaag taatgcatcg acttcagctt cttcaatgtg ttcacgacgt aatttagcga
     4921 acaagttaat taaagagatt aaaataatta gattcaacac accgataagc actaaaaaga
     4981 aacctgaaac tagtgtacca atcgttccac caatatcttg gaaatgcggt aattcatctt
     5041 tagcccattt tacagatacc cctaaaaaaa cagccattaa aaatacgaca gatgaatgtc
     5101 caattgaaaa atagaaaccc acaccagatg gatctttgcg ttgctgtaat aatttgcgaa
     5161 ccgtattatc tattgcagca atgtgatctg catcaaatgc atgacgcaaa cctaatgtat
     5221 atgcaagaat ccccatacca aataagatat gatggtcttt tccagcaatc cataaaaaac
     5281 taaacccaat aacgtgtaac aaaatgacaa tagctatgta tggtaaccag cttaagcgct
     5341 catttttaaa aacagtcaaa atatatcctt ctttcataac atgatattta atcatactgt
     5401 atgttcaatg ggcactctag taataagtgt tcatataaca aaaatgttat gccaaattat
     5461 ttgttatata aaaatataca tgtaaccaca aaagattttt tgcgatatat ataatttgat
     5521 aaattaacca acaacaatgt aagatgtcac tttgcttaac ttggcatcct ttttatgatt
     5581 ttcaaattca aaaaaatgag caaaatgaat ctctttacca gtttttaata tttcaatacc
     5641 atgcatggaa cctaagcacc catgtgtgat gctggaatgg atattgagac tagcaacctg
     5701 attgtaatga ttagatagtt cttgaattaa tatttgaggt ccgtatatgt caaagcggcc
     5761 agggacagac caaataaatt ctgttgtaac cagtgaacgt aataattcaa tatctaatgc
     5821 tgctgtaaca actataaaat ctatcatttg ttgacgttta ggcgcatgat tgcatgacac
     5881 atctcctgtt aacttaaaag gtaatgatga ctgaacttcc gttttaaaat gtagttggtg
     5941 ctgaaataaa gcttgttcca atattgatat agctttagga ccaacgccat gcatctccat
     6001 tagagatgac ttcgtatatt gtgatactgc ttctaatgtg tatatacctt gtgaatttag
     6061 cgcgcgtgtt gcaggctttc caattttagg taaagtcatt tgtatccatc ctttatgttg
     6121 tatggtagtc tttctggcaa tataccctga tttgtacaga attatttata aaattaatat
     6181 tgtttagtgg ttggtgaata aatgtgggaa atataaacaa aatgatatct ttatcgaaat
     6241 attattagta aaatcgttat attagcataa ccaagtgagc gtatgcagta aattaaaaat
     6301 taggaaagta aggaggtttg aaggtgaatt cagagtataa aaaaggcata tttttagcac
     6361 tcagtgcata cattctgtgg ggaatactac ctatatattg gcagttcgtt gatgcaatag
     6421 gcgcatttga aattttagcc tttcgtatta tattttcagc aatattcatg attttcatac
     6481 tcgcggttgg acaaaaacaa cgcaatgcat ttcaacgaga tatgaatcaa ttgttaggca
     6541 agcccattca gctattagcg attgtcgtag caggctatgt cattacatta aattggggta
     6601 catttatttg ggctgtaacg aacggtcacg tcctacaaac aagtttaggt tattatataa
     6661 atccacttgt tagcattttg ctcgcactta tctttttaaa agaaagattc aataaatttg
     6721 aatggctagc cattttattc gcattcatcg gtgtattata tatgacgctc aagattggag
     6781 aattcccaat cgtctctatt atattagcgt tatcctttgg tacatacgga ttattgaaaa
     6841 aagtagtaca tattgatgcc atcagcagta ttacgattga atgtattgtt accgcacctg
     6901 ctggactaat atacgttatt tatttatggc agcaacatca gatgtcattt ggattgaaca
     6961 tgtcatcatt ttggttgtta ttttctggtg ctattacggc aataccacta atcctattct
     7021 cagccggggc aaaacgtatt ccactttcgc taataggatt tattcaatac gttggaccaa
     7081 caataatgtt tgtactcggc atatttgttt tcaaagagcc ttttagtata gatcaattaa
     7141 ttacgtttat atttatttgg acaggtattg tgttatatag tctttctcaa tacattaaat
     7201 tgaagaaaca tccggtcgca aaaaccctat aaaatacgag gaaagataat tatacgtgtt
     7261 ttcaatatcc tttgtgtcat gatgaggtga gactgaggta tgtgtgcagt aacagtgaca
     7321 gacgtataaa tttgaaacat taaatatgaa ataaggcact ttcgttgaat tcgcaattaa
     7381 aatgaattta aacgaaggtg ttttttgtat gatgatttat gagggcatac gtaatgttaa
     7441 tcaatgaagc ggaatagtat agaaatgtat tttatgtgat gttacgtaat gacttaaatc
     7501 tttatacaaa ttttcagtga agcacgcaat gatttaaagg ataaacgtat tttaaaaagg
     7561 gagtcttgat ttagcaaagg ttgattttaa atacaaatta aaaagcgccc aaatcaatga
     7621 gattcgagcg ctaatttaaa tattttaaaa tataccaatt actttcatcc aaagtgatcc
     7681 taaaccaatc cagataataa agtagacgaa acctaatatt aagttcattg tccaccaacg
     7741 tttttgagtc acgtaacctg aagagaatag aatcggcgct ggaccactac tatagtgtgt
     7801 tgttgaagct aataggttac cgaagaaacc taacattaat gcactgaata atggtggtgc
     7861 accggctgcg atggcaacgc ctagtaatgc tgcatacatc gcactgatat gtgctgtaga
     7921 acttgcaaat aagtaatgtg aatagaagta gaacaatatt aaaatgacca ggactatagg
     7981 ccagcttaag ccaccaagac ttgtagcaat ggatttactt aaccaaggaa taaatccaag
     8041 cttgtttaat tggtcggcca ttaacactaa tactgagaac catactaatg tgttccaagc
     8101 acctgtttcg tttaaaatgt cttgccatgt taagacgcct gtcaataata acaatgctag
     8161 cgcaataaag gccgttaaag ttgcatcaat atgaatgaaa cttccgacaa tccatagtgt
     8221 taacgcaacg acaaaaatac caatcataaa tttttcagct aaagcgattt tacccatagt
     8281 cgctaattca ttttcagccc aactcttagc atttggtgtt tctttaacag ttggtggata
     8341 aattttataa ataataaaag gtacaacaat taaggaaaca agtccaggaa ctaaagcagc
     8401 tagaaaccaa ttcatccatg taatgtgaac attagatgtg ctagatgcta aattttgtgc
     8461 aagggggtta ccggccattg cagttaaaaa catagccgca gtaattaaat taccttggaa
     8521 ttctgtgaaa acaagaaatg cacccatttt gcgtgctgat ccgtctttcg gtttcgaacc
     8581 aaatgattca gaaagtgatt tgataattgg gaacatgatt ccaccagcac gcgcggtatt
     8641 acttggtgta gcaggcgcta gaattaaatc tacaccgacg atagaatatg ctaatcctaa
     8701 tgttttttta ccaaataatt tgacgaaatg aagtgcgata cgtctaccaa gacctgtttt
     8761 cacaaatcct ctcgaaatga aaaatgccat agcaattaac caaatgctat tattaccaaa
     8821 accagcgaca gccgttttca tgtcaacaat gccaacgagt accatgattg taaatccaat
     8881 tatagagacg gccccaattg gcatcggttg tgtaatacaa gcaatgattg tcgcgacgaa
     8941 tattgcgaac atataccatg ctgttggatc cacagcatcc ggtttaaaag gtgtaagtgc
     9001 ccaaataagg agacctacaa cgatagggag tataaactta cgatatttaa ccgtgttttc
     9061 catgttaaaa cgtccttctt tctatgtttt atacatattt caatttaaga ataaagctaa
     9121 ctacaaaaga tgtacagtaa taattaaata taaaattaaa ttaacgaaat cattaatata
     9181 attatttttc gagaatcggt gaagaactgg tatagatggt gtttattaaa ttcaaaaaag
     9241 tgacctttta aacaaataaa aaacccgtta acgtgttgtt aacggttaat aagcacatat
     9301 ttattttaaa ttttttaata tacgagacat ctcttcaatt ttaccagcat gaagtgggat
     9361 gtgaatggca tttaatacta ataactcttc taagttatta caaccgacaa ttggctgttt
     9421 caattcaatt gctaaatcat cttcagttag atttcgtgca cgttcaggta aagtttggag
     9481 accttctaaa atcctttcaa tactcggtac ttcatccttc cattcatctg gagacgaacc
     9541 atttccgaat aaaggtctat agatatttaa atcaatattc tctttaccag caacagctaa
     9601 ggccgattca aaaattgtta atacatgacc aaattgccaa tgaatggtat tagaaaaagg
     9661 cttatgataa tcatcaagta ccttttcaac tttccaagca tcatacacat ttactaaata
     9721 tcctaacccc atatcaatga catcaaatac tgtttttgta gtcattatta attcctcccc
     9781 cataaataaa atatacttcc gtaagtatta ccctattaac aaagttgtta gtcatttaag
     9841 gaatgtgtac aacaactacc ttacaataaa agatagttga cgaaaagttc tcaatgtact
     9901 atcttattat gtaagatagt aggtgattaa aatgtcagtg tcagatcaga ttatgaaagg
     9961 tctcttagat ggggcgatat taggtctcat tgcccaaggc gaaacgtatg gatatgaaat
    10021 tatgacgaaa cttaagaatc aagagtttcc agagataagt gagggtagta tttatccagt
    10081 tttaatgcgt ttaaacaaaa aaggatacgt tgatacgaca ataaaaaaat ctagtaatgg
    10141 cggcccgagg cggaaatact atacgattac cgattcaggt ttaaaggaac taaaagtatt
    10201 taaatcgaaa tggcaagttt tgaataaagg gatgattaac ttgtttggag gggaaaacga
    10261 tgttaagtaa acaatctcaa gattttttag aaaaattacg tgttgaattg ttatttagag
    10321 gtaagactga agcagaagta gaagagttat tggaagagct cgaagatcat ttaactatgg
    10381 ctgaaaaaaa taacgaagat acaagttcaa ttattaatac accgattaag tcaatggctg
    10441 atcaattatc acctgagata agtcttacta ccggtttgta taaatatatt actttgtact
    10501 tcgcttttat tttagcagtc attataattc ctaggttttt agatttgggt acatttgatg
    10561 tgacaattgc atttttactt tatatagcta gtatcgttat tttaggtgtt gtcattggta
    10621 tgctgattct acgaaataca ttagtccgat ttggtgacca aaaaataacg tatgttataa
    10681 atgtgactta tggaattatc gtatttttat ggatgatttt aggtggcttg attattaaaa
    10741 aatatcctat ctatagtttc tttgagttaa gtgacaaaca gagtttcatc gttgggttag
    10801 tacttttaat tgcgctcatt ggaacttgta ttttaattaa acagaaaatt tatgcgctca
    10861 tcgtgttggt aatttcattg ccaagtttga tagcaaggat agcagctatc tttagtaaag
    10921 aaccagacac ttttaattta gtttcagtaa gtatactcat cattctaaat atagcattta
    10981 ttatttatac atttgtaaat tttaggaagt taaaaaaaga agactaaaca atgtcgttta
    11041 gctaaaatac atcattatag taaaactgta taccaatcat ccaaaagcac cctaaagtta
    11101 gacttaaact aacaaattag ggtgcttttg tttgtgcact tataactatt ccgttgtgcc
    11161 cagggctagg tgtaaatcct catcattaga aatagcttca atggctgcag tcaaacctgc
    11221 aactatcttt tctaatggca tagatggtgt atcgggttta ccaatgacct gctctggtat
    11281 gtatggcaca tgaataaatc cgaatcgtag gtgagggtaa tgcttatctt gtaaataacc
    11341 taagtgataa agtgtgtgat tacaaacaaa tgtacctgcg ctatttgaaa gtgctccagg
    11401 aagtccttga ttaataatac tttgagtcat tgctttaact ggtaaatttg aaaaataagc
    11461 tggcgcaccg tctaagtgaa tggcttgatc aataggttga aaatcatcat tatctggaat
    11521 acgtgcatca tcaatattaa tggcgacacg ttctggggta atggcatttc taccaccagc
    11581 ttgtcctata gctagtacaa catcataatg attagatgcc aacgttttat ttataatatt
    11641 atctactttc ttaaaagagg ttggtagttt taatttatcg attgtatgtg tgccaataat
    11701 atcttctagt tgagtcacag cttcccatga gggattgata ttttgattgt caaaaggcgc
    11761 aaaccctgtt actaaaatgt gcattttaag acaccccgca gtattaaaat ttaatttagt
    11821 ataacacgcg cataattaaa aacatatcag gttatgtatg cgcataaaaa acagccttga
    11881 aaaaccttta taaatcggat tttcaaagcg tgttttgaaa ggtatttaat taaaactaag
    11941 catgttgatg taaatcatca aagtttgtta aacgttgttg ccactcatta tcactaatat
    12001 tatttgcttg aacataacga ttacgctcat gtttagcaca ttcataagag catgcaccta
    12061 aatatttagt ttcgttttct tcagaaacta atatttgttt attacattct gggttagcgc
    12121 aattaatata acgttcacat ggtttgccat caaaccaatc cttaccaata attgtttttt
    12181 caacttggtt gatatcaaca ctgatacggt catcaaatac gtacatttta ccgtcccaat
    12241 attcaccttt tgtttcagga tctttaccat atgtagcaat accgccatga agttgagcta
    12301 catcttcgaa accttctttt aaaagccatc cagaaaattt ttcgcatcga atgccaccag
    12361 tacagtacgt aaccactttt ttatctgcaa ataacgcttt attctcttta atccagtcag
    12421 gcaaatctct aaaacgtgtg atatttggac gaattgcacc tcggaaatga cctaaatcaa
    12481 attcataatc attacgtgca tcaataatga ctgtgtcatc atcttcaaga gcttttctaa
    12541 attctacagg tgataaatat tggccagttg tgtgtcttgg atcgacgtca tcttctaaat
    12601 ctaaagcaac gatttctttt cgaggacgta catgcatttt cttaaaagca tgtccttcag
    12661 cttcatcaat tttaaacacc atatctttga atcgttcatc ggcatgcata tgtgccatat
    12721 attgttcggt ttcttctttt gtaccagata atgtgccgtt aataccttct gtagaaacaa
    12781 gaattctacc ttttaaatgg tgtgctttac aaaaggctaa gtgatcctga gcaaactgtt
    12841 cagggtcatc aatcgtcata tatttataat ataaaagaac ttgatagttc ataggttaat
    12901 ctccttctat aaacatgtta taagcatgtt aatttttaaa ataaagcgtc aatacgcata
    12961 tttgtctacg tccatcattt taacagagtt gtattgtcat tcaatatttc tagcttgaaa
    13021 tcagccacta gaccgataaa atagatagat gaaaccgtta tcattttatt tgatagttat
    13081 cttgtttaaa ataaaattga tagttaaaac gaatgaaatg aaaggaatga tcatgatgtc
    13141 acaacaagat ttacctacat tattttatag cgggaagtcc aatagtgctg ttccaattat
    13201 atctgaaagt gaattacaaa caattacagc tgaaccatgg cttgaaattt ccaaaaaagg
    13261 attgcaacta gaaggattga actttgatcg gcagggacaa ctctttttat tggatgtatt
    13321 cgaaggcaat attttcaaaa tcaatcctga aacgaaggaa atcaaacgac cttttgtaag
    13381 tcacaaagcg aatcctgcag caatcaaaat acataaagat ggccgattat tcgtttgtta
    13441 tttaggagat tttaaatcta caggaggcat ttttgcagct acagaaaatg gtgacaactt
    13501 acaagatatt attgaagatc tttcaacagc atattgtatt gatgacatgg tatttgattc
    13561 taaaggtgga ttttatttta cagattttag aggatactct accaatccac taggaggcgt
    13621 ttattatgtt tcgccggact ttagaacagt gacgcctatc attcaaaata ttagcgtagc
    13681 aaatggtatt gctttaagta cagatgaaaa agtactatgg gtaacagaaa caacagccaa
    13741 tcgattacat cgcattgcac ttgaagatga tggtgtgacg atacaaccat ttggagctac
    13801 cataccgtac tattttacag gtcatgaagg accagactca tgttgtattg atagtgacga
    13861 taatttatac gtagcaatgt atggtcaagg tcgagtgtta gtttttaata aaaggggtta
    13921 tccaatagga caaatattga taccaggccg agatgaaggg catatgttac gttctactca
    13981 tccgcaattt atacctggaa caaatcaact catcatttgt tccaatgata tagaaatggg
    14041 cggaggatct atgctttata cagttaatgg ctttgcaaaa ggtcatcaaa gttttcagtt
    14101 tcaataagac ctacctgaat gaaaggcgtc taaaattagg gtgaacagtt agtgcttgtt
    14161 acacatgagt taaacgaaaa atgccaacta tacctaggga tgatatagtt ggcatttcgt
    14221 atatatatta aaacaatgag gattgtgtaa tgagattatt ctgagattga gaattcagct
    14281 gatgcttcga atttaacatc tttgcctagc attacgccac cagtttcaag tgcttggtta
    14341 aagttaatgc catatttttc tctattgatt gtaccagtaa caataatacc tgttacttga
    14401 gaaccatcca taggattctt acttactccg ttgaattcaa catcgaatgt ttcttcgtta
    14461 gtgatgcctt taattgttaa atcaccaaca actttgcttt cagatactga ttttgtcaca
    14521 aatgtaattt tatcaaattc gtcagtacca aagaaatcac cagattttaa gtggttatca
    14581 cgtgcttcgt ttttagtgtt aattgagctt ggaataattg ttgcagtagc tttcaaagta
    14641 ctgaagtcat taatatctcc ttcaacagct acatcaaatt gatcaaatga acctttcact
    14701 ttagaaacca ttaaatgttt aatttggaat tctaaactac tgtgtgcacc atcaaaagta
    14761 aagttagtca taatataaaa cctccatatt tttatctctt agatatattt tttatacttg
    14821 atatacatac aataccacct aaaaaacaaa gatgcaagca ttttatttcg aattcgaata
    14881 ttttctgtga tgtatttaaa gaatgaccta taaaacacca cactattgtt ataccctagt
    14941 caattaattc aaacataaag aagtgattac aagtccgtga aatttagtta atgagataaa
    15001 tctgactatt attatcattt ttatagtcac tactattgac tttgcatcaa gtgactcgta
    15061 ctattttaat ataattctga aaattcaaaa tatagaacat taaatatgta gataatattt
    15121 ttttgaagtt ttaaaaaaag gaggcatttt atgggaaatt atttggtatt gcaatcagat
    15181 tttggactta gtgatggcgc tgtaagtgca atgtatggag ttgcatatac tgtaagtgat
    15241 gatatacgtg ttgaaaactt aacgcatgat ataccaccgt atgacatttg ggtagcgtca
    15301 tatcgtttat atcaaactgt aaaatactgg cctaaaggca cagtttttgt atcagtggta
    15361 gacccgggtg taggtagtga taggcggagc attgcttgct taacatattc aggtcattac
    15421 attattacac ctgataatgg ttctttaagt catattaaac attacgaagg tattaaaaaa
    15481 gttatcgaaa ttgatgaagt gaaaagccga ttgcctcatt cggaagaaag tcatactttt
    15541 catggtagag atgtttatgc atacaatggt gcgagattag ctgcaaataa gatagcgttt
    15601 gaacgtctcg gtcaggcaat taatgttgat agtattgagg cattagaaat aagagaagtg
    15661 acaaaaaatg aggattctgt tacaggaagc attgatattt tagatatcag atttgggtca
    15721 ttatggacaa atattccatt ggcattctta aaatccttgg aaattgttca tggtaataat
    15781 ttggtggtaa caatttatca tcaagataag aaagtctatc aaaatattat taaatttgcg
    15841 cgatcgtttg cagatgttaa tgtgggtgaa cctcttgtgt atgttaactc gctagtaaat
    15901 attggtgtag cagtcaatca agactcattt tcagatttat atcatattgg tactggaaat
    15961 gattggacga ttcatttatc taaagcacca tcaatataat gaatttttta tgtaaggagt
    16021 aatgaagatg aaaaaacaag atatttcagt taaaactgtt gttgcaattg gtataggagc
    16081 agcggtattt gtaattttag ggcgttttgt tgtaatacca acaggttttc ctaatacgaa
    16141 tatagaaaca tcatatgcat ttttagcatt aatatctgca atatttggac cttttgctgg
    16201 tttaatgact ggattagttg gacatgctat taaagatttt acgacatacg gtagtgcctg
    16261 gtggagttgg gttatttgct cgggaattat aggctgttta tatggctgga ttggactaaa
    16321 attaaatctt tcttcaggcc gattttctag gaaatcgatg gtttatttta atatcggtca
    16381 aattattgcg aatattattt gctgggcact tattgcacca acattagata ttttgattta
    16441 taacgaacca gctaacaagg tttatacaca aggtgttatc tctgcagtat taaatattat
    16501 ttcagttggc attattggga caatattatt aaaagcatat gcttcatctc aaattaaaaa
    16561 aggtagttta cgtaaggaat agttattttg ttgaatcaga tatgtaaatg aatatagaaa
    16621 ggtaatgata tatcatgact gaaccaatta tctcgttcaa agactttagt tttcaatatc
    16681 atagtcaagc aacacctaca ttacagaata taaatgttga tatttatcca ggagaaaaag
    16741 tattagtagt tggtgcttcg ggtagtggta aatcgacttt tgcaaattgc ataaacggat
    16801 taattccatt taaaactaaa ggtaacataa ctggggaact atatataaat aatcaagatg
    16861 caaccgttag ttgtttacat gatagatcta atgttgttgg tacagtttta aaagatacag
    16921 atggacagtt cataggctta acagcagctg aagatatggc ctttttatta gaaaataatt
    16981 gtgttgaaca agatgatatg aagaaaaatg taagttattg ggctgaaaaa gttggcatga
    17041 tagaacattt aaatcaccga ccgcaagatt tatctggagg tcaaaaacaa cgcgtttcat
    17101 taggtggtat attaatccat cgtacgccta ttttaatatt ggatgagcca ctggccaatt
    17161 tagatcctgc gacaggacat gaaacgctga gattgttaaa caatattcat gaagaaacaa
    17221 agtcaacgat gattattgtc gaacatcgat tagaagaatc attagatgat acgtttgata
    17281 gagttcttct ttttaaagat ggaaaaatca tcgctaatac aacgcctagt gatttgctaa
    17341 aatcatctaa gcttaaagaa gcaggcatac gtgaaccatt atactgtaca gcgcttaaat
    17401 atgcagaagt agatgtcgaa tccattgata acttagcgaa tttacgtgac gtttgtatga
    17461 gtgaacatgt taaatttaaa gtgaaaaaat ggatagatga aacatctgca aataatgata
    17521 ataagtacaa atctgaaccg ctattagaat taaatgaggt atgtgttcaa tatagtgact
    17581 atagtaatag tgtacttaat aatgttcaat taaatgttta tagaagagaa atgcttagta
    17641 tagttggtca caatggtgcc ggcaaatcaa cacttgctaa agcaatttgt ggatttttag
    17701 atataacagg taatatccaa ttttgtaata ggggatttaa tcaattgtca attagcgaac
    17761 gatctgaatt cgtaggttat gtgatgcaaa atccaaatca tatgatttct gaaaaaatga
    17821 tttacgatga agtagcatta gggttaagag cacgaggtat gaaagaatca gatataaaga
    17881 tacgagtaga gaatgtgctc aaaatatgcg gactttatgc atttcggaat tggccaattg
    17941 cagctttaag ctatggtcaa aaaaagaggg tcactatagc atctgtttta gtcttaaatc
    18001 cggaaataat catattggat gaaccgactg ctggtcaaga tttctatcat tataatgaga
    18061 taatgtcatt tttaattgaa ctaaacagac aggggaagac gattattatg attacgcatg
    18121 atatgcattt attgtctgag tatagttcaa gaacagttgt attatcaaaa ggtcaagtcg
    18181 ttgctgatac cacgccagta ttggttttaa atgataaaaa aatctgtgag attgcatcat
    18241 tgagacaaac atcgctattt gaaatggccg aatatatagg gattagcgag ccacagaaat
    18301 tagtacaatt atttattaac catgatagga aggtgagacg ccaatgaatc aatataatac
    18361 tataggtttt cacccgggaa atagtcgtat tcatcaatta aatgcgactg ttaaactttt
    18421 attcttatta gttgtttcta tttctgcaat ggtgacttat gacacaagat atttaatttt
    18481 aattagtgct tcatctattt tattggtcaa atatgctcat attgaatgga aacaagttcg
    18541 ctttgttgtt aaattcattc tgtttttcac aatattaaat attattgccg tgtacatatt
    18601 tgaccctgaa tatggtgtga agatttataa tcagcgtaca gagttagtca atggtattgg
    18661 tcgatttacg ctaacatcac aggaattatt ctatcttttt aatctaatat taaaatatat
    18721 tagtacagtt cctttagcgt taatattttt attcacaacg aatccgagtc attttgctgc
    18781 aagtttaaat cagctaggtg tgaattataa aatcagttac gcagtctcac tagcattaag
    18841 gtatattcca gatattcaag aaacatattt taatatttca caagcgcaac aagcaagagg
    18901 atatgataat tcgaaaaaag caaaatttac tagccgtgtt aaaggtatca aacgcattgt
    18961 gttaccttta atattttcta gtatcgaaag aattgacact attagtactg ctatggagtt
    19021 aagacaattc ggacagtata aaaggagaac ctggtacgtc aaaaaacaat taaaaaaaga
    19081 tgattatgtt gttttgtgtt tgacgttaat acttctgatg ttagtagtta cattattctt
    19141 tttaaataat agtcgatatt tcaacccgtg gcattagtat tcatataaat agtctttaaa
    19201 tagaaatagg agggagacat ttaatgataa atactgaaag attaaattta atgattccaa
    19261 gttcctcgca tttaattgaa ctttataata tttgtagtca tccacaagca aatatataca
    19321 ctcccaaagg tttacataat tccaaattag acacacaacg gtggattgaa aaatggcgaa
    19381 accattggca acaatatcaa tttggttact ttgtattggt aaaaaaaata gattgtagtg
    19441 ttattggtat ttgtggatat gaatatcgac aattaaagca agaaacagta ttaaatttat
    19501 tttataaatt acatccaagt tttgaaggac aagggtacgc atgtgaggct attacagcaa
    19561 tcacaaattt tgtgaattat atcgatcaag aaacagtaaa agttatcagg acaaataagt
    19621 gtaaccaacg ttcaataaat ttagcagaaa ggcttaaatt caagcgagac gatactatgg
    19681 acgacattat caatcaagga gatattgtgt tttataaata aaatactatg acattatcta
    19741 aaaaataaaa ttaaaatccc aacagtttct taaaaagtta tgagccacat aataaaaatg
    19801 ttataattgt gcataataaa acatagggag aatgtttaat gcataaaaaa tatttattgc
    19861 tattttttct gttattatca gttgcattgt tatatagctg ttccaagcaa aatgagaaag
    19921 ctcaaaataa atctgttaat ccaaatgagt atgcaaagca taaatcgaaa atggatgaac
    19981 attgggaaac atatcacggc caagtttatc atgtgtttta tcacccatta attacagacc
    20041 caaaagttgc gtttacagga gatacacacc aagctaaggg caacaatgat tggatgatta
    20101 ctgtaagtga atttaaaaaa tcattgaacg aattatataa aaataattac atcatcgtta
    20161 atccacatga tgtatacgac ttatcatctc atcatgtaaa agccaaaaaa ataaaattgc
    20221 cgaaaggtaa aaagccacta atcatctcaa ttgatgatat gaattactat agttacatga
    20281 gacaccatgg ttatgctgac cgtttagtac tcgataaaaa taaacatgtt gtttctgaaa
    20341 ctacagacaa aaaaggccat gctacttatt ctgatgacaa tgatatcgtg ccaattttaa
    20401 ataaatttgt aaaagagcat ccagattttt ctttaaatgg tgaaaaaggt gttgttgctt
    20461 taaccggtta tgagggcgta ttaggttatc gcacgaatga attaacgagt aaagattata
    20521 caaagaataa aaaggcagct gaggaagttg ttcgcgcaat gaaacgggat ggatggtcat
    20581 ttgcgagtca ttcatatggt cacattaatt ttgaaaaaac atctttagaa ggtattaaac
    20641 gtgatactaa aagatggaaa gatgaagttg aaccaattgt aggtaaaaca gatatgtttg
    20701 tatttccaca tggcgcacag gatagacata cacaagcgta tgattatttg gtagatgaag
    20761 cagagtttaa gtttattgca ggtgtcggtc caaacaattt tacagatata tcagcaacga
    20821 acgtttacca agatagggtt gcgattgatg gtctaaactt atttgaattt aaatataaac
    20881 tcaaaccgtt ttttaatcct gaaaatgtat atagtaaaca agatagacgt tactttaaag
    20941 ggaatcggga ttatgaagaa tagttaaatt taataaaaag atagaaagaa aataacttta
    21001 cataataagg caaggacaat atgtgcaaga agtacaactt caatataaaa atcttggtta
    21061 agcattttta taggcacaaa tgtcttgcct tttttatttt aaaaaatttt aaaatcaata
    21121 caccgaattt tctgaaaaag gttgtaatgt attgctaaaa atgataagtt attaatcata
    21181 ttacacttta gcgtggtaga gaagtaaagc aaacaacgaa aataccaagt gattttaaat
    21241 acagtattga aagtaaaggg tggtcgagtg aataattcag aacaattaat tgcgttaaaa
    21301 gaatcggaaa cagcattttt aaaatatttt aataaagcgg attatgagtt agttgatttt
    21361 agtgtggttg aaaagttgga ctggaaacaa ttgaaccatg aggatttgca gcagatgggt
    21421 gagcgaaatt tttggcaaca tgagcatcaa atttatgcac ttcgaaatga ttttacagat
    21481 caattattgc gttattactc aatgtatcca actgcagcaa caaaagtggc atacacaggt
    21541 ttaatcattc ggaataatga agcggctgta caagttggtt tggaaaacta tgcaccatca
    21601 ttagcgaatg tgcaacagag cttaaagctc tttattcagt ttattcaaca gcagttgaga
    21661 gacaacgtgc attttgttgt attgggacat tatcaattgc ttgatgcctt actcgataaa
    21721 tcgttacaaa caccagacat actatcaatg attgaggaac gtaatttatc gggactggtt
    21781 acatatttgt cgactgaaca tccaatcgtg caaatcttga aagagaatac gcaacaacaa
    21841 ttaaatgtat tggaacacta tattccaaat gaccatccag cactcgttga attaaaaata
    21901 tgggaacgtt ggttacataa acaaggttac aaagacatcc atttagatat tactgcgcag
    21961 ccacctagat cctattacac aggtttattt attcaatgtc attttgctga aaatgaatct
    22021 cgagttttaa caggtggtta ttacaaagga agcatcgaag ggtttggatt aggattaaca
    22081 ctttaagtaa gggagtatgc acaatgttaa gaatcgccat agccaaagga cgtctaatgg
    22141 atagtttaat taactattta gatgtaattg aatatacgac attatcagaa acattaaaaa
    22201 atagagaacg ccaattatta ttaagtgtag ataatattga atgcatttta gtaaaaggaa
    22261 gtgacgtgcc aatctatgtg gaacaaggaa tggcagacat aggcattgtt ggtagcgaca
    22321 tattagatga gcgccaatat aatgttaata atttgttgaa tatgcctttt ggagcatgtc
    22381 attttgcggt tgcagcgaaa cctgaaacga ccaattatcg taaaatcgca acgagttatg
    22441 ttcatactgc tgaaacatat tttaaatcaa aaggtattga tgtcgaattg attaaattga
    22501 atggctctgt tgaattggcc ggtgttgtag atatggtaga cggaattgtc gacatcgttc
    22561 aaacaggtac tacgctaaaa gcgaacggac tggttgaaaa gcaacatatt agtgatatca
    22621 atgcaagatt aataactaat aaagcagctt attttaaaaa atcacaatta atagagcaat
    22681 ttattcgctc tttggaggtg tctattgcca atgcttaatg cacaacaatt tttaaatcaa
    22741 ttttcattag aagcaccatt agatgagtca ttgtatccaa ttattcgcga tatttgtcag
    22801 gaagttaaag ttcatggaga taaggctttg aaaatgtata atctaacatt cgatcatacg
    22861 aaaacagatc atttagaaat tagtcatgaa caaattaaag cagcatttga cacattagat
    22921 gaaaaaacaa aacaagcatt acaacaaagt tatgaaagaa ttaaagcata tcaagaaagt
    22981 attaaacaga cgaatcaaca gttagaagaa tcagtggagt gttatgaaat ataccatcca
    23041 ctagaaagtg tcggtattta tgtgcctggt ggtaaagcaa gttatccttc aacggtatta
    23101 atgacagcga cgttagcaca agtagcaggt gtggaaaata ttgtcgttgt gacaccacct
    23161 caacctaatg gtgtgtctca agaagtatta gcagcatgct atattacgca agttgatcaa
    23221 gtatttcaag ttggtggtgc tcaaagtatt gctgcgttaa cttatggcac agaaacgata
    23281 cctaaagttg ataagattgt aggtccaggc aatcaatttg ttgcatatgc caagaaatat
    23341 ttatttggac aagtaggtat tgaccaaata gctggaccaa cagaaatagc acttattatt
    23401 gatgaaacag cagatttaga tgccatcgta tatgatgttt ttgcacaagc agaacatgat
    23461 gaattagcac gtacatatgc cattagtgaa gatgcgcaag tccttaaaga tttagaatca
    23521 cgtattgcta aagcattgcc taatgtggac agatacgaca ttgtttctaa aagtatcgct
    23581 aatcaacact accttatcca tgctagtaat tttgatgaag catgccatgt catgaataca
    23641 atcgcgcctg aacatgcgtc gattcaaaca gtaaatcctc aaccatatat tgaaaaagtg
    23701 aaatatgtgg gtgcattgtt tattggacat tattcgccag aggtcatagg agattacgtt
    23761 gcaggtccaa gtcatgtatt acctacaaat agaacagcta gatttaccaa tgggttatcg
    23821 gtcaatgatt tcttaacacg gaacacggtc atccatttat caaaagatac gtttgaacaa
    23881 attgctgatt cagcacaaca tattgctcat gttgaagcat tatacaatca ccagcagtct
    23941 attttaatac gtcagtctta ggggagtgta attgaaatga tttatattga taaaaatgaa
    24001 agtccagtta cgccgttgga tgaaaaaaca atgacgtcta ttattagtgc aacgccatat
    24061 aatttatatc ctgatgcagc atatgaacaa ttcaaggaag cttatgctaa gttttacgga
    24121 ttatcgcctg aacaaattat tgcaggaaat ggatctgatg aattgattca aaagttaatg
    24181 ctgatcatgc cagaaggtcc ggcattaacg ctaaatcctg atttttttat gtatcaagca
    24241 tatgcggcac aagtaaatcg tgaaattgca tttgtagatg caggatcaga tttaacgttt
    24301 gatttggaaa ccattttaac gaaaatcgat gaagtacaac catcattttt tattatgagt
    24361 aatccacata acccttcagg caagcaattt gatacggcat ttttaacagc tattgcagat
    24421 aagatgaaag cattaaacgg atactttgtc attgatgaag catatttaga ttatggtacg
    24481 gcatatgacg tggaactggc accacacatc ttaagaatgc gtacattatc aaaggcgttt
    24541 ggaattgccg gcttaagatt aggtgtctta attagtactg ctggaacgat aaagcatatt
    24601 caaaaaatag aacatccata tccattaaat gtatttacgc taaatattgc gacttatatt
    24661 tttagacata gagaagagac aagacaattt ttaacgatgc aacgacagtt agctgagcag
    24721 ttaaaacaaa tatttgatac acatgttgca gataaaatgt cagtgttccc atcaaatgct
    24781 aattttgtac ttactaaagg ctcagcagcg caacaattag gacaatacgt atatgaacaa
    24841 ggatttaaac ctcgctttta tgatgagccg gtgatgaagg gctatgtaag atactcaatt
    24901 gcaacagcat cacagttaaa gcaattagaa gaaattgtta aagaatggag tgcaaaatat
    24961 gatttatcaa aaacaacgaa acacagctga aacgcaacta aatatttcaa tatctgatga
    25021 tcagtcacca tcgcatatta atacaggtgt gggcttttta aatcatatgt taaccttgtt
    25081 tacatttcat agcggtctgt cattaaacat tgaggcacaa ggtgatattg acgtagatga
    25141 tcaccacgta actgaagata tcggcattgt cattggccaa ttgttacttg aaatgattaa
    25201 agataaaaag catttcgttc gttatggaac gatgtacatt ccaatggatg aaacattagc
    25261 acgtgtcgtt gtggatataa gtgggcgccc atacctatca ttcaatgcat cattaagtaa
    25321 agaaaaagtt ggtacgtttg atacggagtt agtagaagaa ttttttagag cggtcgtaat
    25381 caatgcaaga ttaacaacgc atattgattt aattcgtgga ggtaatacac accatgaaat
    25441 tgaagctata ttcaaagcgt tttcccgtgc attaggcata gcgctaactg caactgatga
    25501 tcagcgtgtg ccgtcatcga aaggtgtgat tgaatgattg tcatcgttga ttatggatta
    25561 gggaatatta gtaatgtaaa acgcgctatt gaacatttag ggtatgaggt ggttgtctca
    25621 aatacctcaa aaataatcga tcaagcagaa acaatcatat tgcccggtgt cggccatttt
    25681 aaagatgcga tgtcagagat aaaacgatta aatctcaatg caatattggc taagaatact
    25741 gataagaaga tgattggtat ttgtttaggc atgcaattaa tgtatgagca tagtgatgaa
    25801 ggcgatgcat ctggattagg gtttatccca ggaaatattt cgcgtatcca aacagaatac
    25861 ccagtgccac acttaggctg gaataattta gtgagtaagc accctatgtt aaatcaagat
    25921 gtttatttcg tacattctta tcaagcgccg atgtcagaaa atgtaattgc atatgcgcag
    25981 tatggggctg atattccggc aattgttcaa tttaacaatt atattggtat tcaattccat
    26041 cctgaaaaaa gcggtacata tgggttacaa attttgcgtc aggcaataca agggggattt
    26101 ataaatgatt gaattatggc cagcgattga tttgattggg tcaacaagtg tgaggttaac
    26161 agagggtaaa tatgatagtg aagaaaaaat gtcacgctcg gctgaagaaa gtattgctta
    26221 ctatagtcaa tttgaatgtg tgaatcgtat tcatatcgtc gacttgatag gtgctaaggc
    26281 acagcatgcc cgagagtttg attatattaa gtcattgagg agattaacaa ccaaagatat
    26341 tgaagtaggt ggtggcattc gtacgaagtc acaaatcatg gactactttg ccgcagggat
    26401 taattattgc atagttggaa cgaaaggtat tcaagatact gattggctta aagagatggc
    26461 acatacattt ccaggtcgca tttatttatc tgttgatgcc tatggagaag atattaaagt
    26521 gaacggatgg gaagaggaca cagagttaaa tttatttagt tttgtgagac ggttatcgga
    26581 tatacctctt ggcggcatta tatatactga tattgctaaa gatggcaaaa tgtccggacc
    26641 taactttgaa ttaactggtc aattagtaaa ggcaacgacg attcctgtca ttgcttccgg
    26701 tggtattaga catcagcaag atattcaacg attagcgtcg ctaaatgttc acgctgctat
    26761 tataggaaag gctgcacatc aagcatcttt ttgggagggg ctaaaatgat taaaaaacgt
    26821 atcattccat gtttagatgt caaagatggt cgtgtcgtta aagggattca atttaaagga
    26881 ttaagggata ttgggaatcc tgttgattta gcaatgtatt acaatgaagc gggtgctgat
    26941 gaattagtat ttttagacat ctctaagacg gaagagggtc atagcttaat gctagaagtg
    27001 attgaacaga cagcgtcacg cttgtttatc cctcttactg tagggggtgg gattcaaagt
    27061 ctcgatgata ttacccaatt gctaaatcat ggtgcagata aagtatcatt aaattcaagt
    27121 gctttaaaaa atccacagct cattaaacaa gcgagtgata aattcggtag acaatgcatc
    27181 tgcatagcaa ttgatagcta ttatgatcct gaaagaaaag cacattattg ttgtacgact
    27241 ggtggtaaaa aaatgacaaa tattaaagta tatgactggg tacagcaagt agaacagtta
    27301 ggtgcaggtg agctcctcgt tacaagtatg ggacatgatg gtatgaaaca aggctttgat
    27361 attgaacacc tagcaaatat taagtctctt gtaaatattc caatcattgc ttctggtggt
    27421 ggtggcaatg cacaacactt tgtagaatta tttgatcaga cggatgtttc tgcaggttta
    27481 gctgcaagta tattacatga tcgagaaacg acggttcaat ctattaaaga agtgatacgg
    27541 caagggggta tagcagtaag atgaccaaat ataaaattga ttttagcaaa ggtttagtgc
    27601 cagcaatttt acaagataat caaacaaaac aagtattgat gttgggttat atgaaccaag
    27661 aagcttttga taaaacgata gaagatggtg tggtatgttt ctattcgcgg tcgaaacaac
    27721 ggctatggac aaaaggtgaa acatctggtc atacgcaacg tgttaaagat attcatgtag
    27781 attgcgacaa tgacactatt ttaattgatg tcataccaaa tggaccaaca tgtcatacag
    27841 gcagtcaaag ttgtttcaac acagaagttc cattttcagt gcaaacatta gcgcagacag
    27901 ttcaagatag tgcccaatcc aataatgaaa agtcatatac aaaatattta ttaacagaag
    27961 gtatagaaaa gattacaaaa aaatacggtg aagaagcttt tgaagtcgta attgaagcaa
    28021 ttaaaggtga caaaaaagca tttgtaagtg aagtagcaga tgaactttat catttatttg
    28081 tcttgatgca tgcgcttggc gtcgattttt cagaaattga ggcggaatta gcgcgtagac
    28141 atcataagcg caataacttt aaaggtgaac gacaaaatat cgaacagtgg taaagcaagt
    28201 atggactaag atataaggaa aaggatcatg gcttatacac ttacaaatat tgtggaaaac
    28261 gtgacatttt caagtttaaa atacgacacc aacatatttt aactatgaat gctgtgatgg
    28321 tactaaagtt gcgaactcgt tatagataag tagtggataa tcacaatacg aaatcaaaaa
    28381 taattataaa aagtaaattg agcaactcag gaatagatgt cactgttaaa gatgtcgaaa
    28441 agtatatgaa tcgatataat gaagttatga agggaaaaaa tggcgaaaaa gctaaagagt
    28501 tatgtttgtc gttactacct attaatatca tagttgtctt tacattcttt gtatttatac
    28561 tataaataca aatatatcta gcctgaaata gaaatgtcat agcctattta aaagacaatc
    28621 tccattagaa ctaagatatg catcccgaaa gttagactaa aaaactaact ttatgggatg
    28681 tatttttatg ctaatcatca taaattcgag attaagttaa aggtagttca agagtaatta
    28741 aacaataaat taaaaatagt aggatactta ctttgaggga agaaaattaa ctgtatatat
    28801 ttagtttagg aacaagtatt acggtttatc ctgatacaat tattgtggat gggatgatat
    28861 ttttaggttt aaaatacgac accagcaaac ataataactg taatagctca taaatctccc
    28921 catatagcta atctaaaaaa ataatacatc attggaatta agccccaagc atgtaaatat
    28981 taaaaatcaa aaaagatata tgtaaaaaag ttacaatttg cataattaaa ttgtgtctaa
    29041 ttattgacta attaaatttt gccaaatata atattaatta ataatttgaa atgattagcg
    29101 tatacacttt aaattctctt tggagaatat attttttaaa tacaaatgta aacgctttct
    29161 cgtcaaatta aacaatagaa aggatggtca ttatgagtgc ttggttaagt aaattatttg
    29221 agtttattcc tcgaataatt atcaatttgt ttatctaaaa taaaaaaata gaggtgctga
    29281 caatgatgaa aagtcaaaat aagtatagta ttcgtaaatt tagtgtaggt gcatcttcca
    29341 ttttaatagc tacattacta tttttaagtg gtggacaagc acaagcagct gagaagcaag
    29401 tgaatatggg aaattcacag gaggatacag ttacagcaca atctattggg gatcaacaaa
    29461 ctagggaaaa tgctaattat caacgtgaaa acggtgttga cgaacagcaa catactgaaa
    29521 atttaactaa gaacttgcat aatgataaaa caatatcaga agaaaatcat cgtaaaacag
    29581 atgatttgaa taaagatcaa ctaaaggatg ataaaaaatc atcgcttaat aataaaaata
    29641 ttcaacgtga tacaacaaaa aataacaatg ctaatcctag cgatgtaaat caagggttag
    29701 aacaggctat taatgatggt aaacaaagta aagtggcgtc acagcaacag tcaaaagagg
    29761 cagataatag tcaagattca aacgctaata acaatctacc ttcacaaagt cgaataaagg
    29821 aagcaccatc attaaataag ttagatcaaa caagtcaacg agaaattgtt aatgagacag
    29881 aaatagagaa agtacaacca caacaaaata atcaagcgaa tgataaaatt actaactaca
    29941 attttaacaa tgaacaagaa gtgaaacctc aaaaagacga aaaaacacta tcagtttcag
    30001 atttaaaaaa caatcaaaaa tcaccagtag aaccaacaaa ggacaatgac aagaaaaatg
    30061 gattaaattt attaaaaagt agtgcagtag caacgttacc aaacaaaggg acaaaggaac
    30121 ttactgcaaa agcgaaagat gatcaaacga ataaagttgc caaacaaggg cagtataaaa
    30181 atcaggatcc tatcgtttta gtgcatggtt tcaatgggtt tacagatgat attaatcctt
    30241 cagtgttagc tcattattgg ggcggtaata aaatgaacat tcgccaagat ttagaagaaa
    30301 atggttacaa agcttatgaa gcaagtataa gtgcttttgg aagtaactat gaccgcgcag
    30361 ttgaacttta ttattatatc aaaggcggtc gtgtagatta tggtgcagca catgcagcaa
    30421 aatatggaca tgaacgttat ggaaaaacat acgaaggaat ttacaaagac tggaaaccag
    30481 gacagaaggt acacctagtt ggacatagta tgggcggtca aacgatacgt caactagaag
    30541 aattactgcg taatggtaat cgtgaagaaa tagagtatca aaagaaacat ggtggcgaaa
    30601 tttctccact attcaaaggt aatcatgaca atatgatttc atcaattact actttaggaa
    30661 caccacataa tggtacacac gcatcagatt tagctggtaa tgaagcttta gtgagacaaa
    30721 tcgtatttga tatcggtaaa atgtttggta ataaaaattc aagagtagac ttcgggttgg
    30781 ctcaatgggg tctaaaacag aagccaaatg aatcatatat tgattatgtc aaacgcgtta
    30841 aacaatctaa tttatggaaa tcaaaagata atggatttta cgatctgacg cgtgagggtg
    30901 caacagattt aaatcgtaaa acgtcgttga accctaacat tgtgtataaa acatacactg
    30961 gtgaagcaac gcacaaagca ttaaatagcg atagacaaaa agcagactta aatatgtttt
    31021 tcccatttgt gattactggt aacttaatcg gtaaagctac tgaaaaagaa tggcgagaaa
    31081 acgatggttt agtatccgtt atttcttctc aacatccatt taatcaagct tatacaaaag
    31141 cgacagataa aattcaaaaa ggcatttggc aagtgacgcc tacaaaacat gattgggatc
    31201 atgttgactt tgtaggacaa gacagttctg atacagtgcg cacaagagaa gaattacaag
    31261 atttttggca tcatttagca gacgatttag tgaaaactga aaagctgact gatactaagc
    31321 aagcataatt tataaagtaa agggaggaat taataatgac tgcagacttc tttcaattaa
    31381 tcggatcatt atttagaatt ctaaaagaat tattcaagta aaacattggc g
//
LOCUS       DANRDI010000016        29067 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.016, whole genome shotgun sequence.
ACCESSION   DANRDI010000016 DANRDI010000000
VERSION     DANRDI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29067
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.016"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            417..602
                     /locus_tag="PZ377_000544"
     CDS             417..602
                     /locus_tag="PZ377_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357435.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(1255..1605)
                     /gene="scb"
                     /locus_tag="PZ377_000545"
     CDS             complement(1255..1605)
                     /gene="scb"
                     /locus_tag="PZ377_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HDM6357436.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            complement(1759..2256)
                     /gene="efb"
                     /locus_tag="PZ377_000546"
     CDS             complement(1759..2256)
                     /gene="efb"
                     /locus_tag="PZ377_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HDM6357437.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            complement(2515..3021)
                     /locus_tag="PZ377_000547"
     CDS             complement(2515..3021)
                     /locus_tag="PZ377_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357438.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            3693..4094
                     /locus_tag="PZ377_000548"
     CDS             3693..4094
                     /locus_tag="PZ377_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDM6357439.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            4054..4146
                     /locus_tag="PZ377_000549"
     CDS             4054..4146
                     /locus_tag="PZ377_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357440.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(4463..4792)
                     /gene="ecb"
                     /locus_tag="PZ377_000550"
     CDS             complement(4463..4792)
                     /gene="ecb"
                     /locus_tag="PZ377_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HDM6357441.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
     gene            complement(4986..5153)
                     /locus_tag="PZ377_000551"
     CDS             complement(4986..5153)
                     /locus_tag="PZ377_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357442.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(5281..5784)
                     /locus_tag="PZ377_000552"
     CDS             complement(5281..5784)
                     /locus_tag="PZ377_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDM6357443.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(5777..6364)
                     /locus_tag="PZ377_000553"
     CDS             complement(5777..6364)
                     /locus_tag="PZ377_000553"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDM6357444.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(6376..7176)
                     /gene="racE"
                     /locus_tag="PZ377_000554"
     CDS             complement(6376..7176)
                     /gene="racE"
                     /locus_tag="PZ377_000554"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDM6357445.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(7412..8227)
                     /gene="sdhB"
                     /locus_tag="PZ377_000555"
     CDS             complement(7412..8227)
                     /gene="sdhB"
                     /locus_tag="PZ377_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDM6357446.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(8227..9993)
                     /gene="sdhA"
                     /locus_tag="PZ377_000556"
     CDS             complement(8227..9993)
                     /gene="sdhA"
                     /locus_tag="PZ377_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDM6357447.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(10045..10659)
                     /locus_tag="PZ377_000557"
     CDS             complement(10045..10659)
                     /locus_tag="PZ377_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDM6357448.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(10983..12764)
                     /gene="uvrC"
                     /locus_tag="PZ377_000558"
     CDS             complement(10983..12764)
                     /gene="uvrC"
                     /locus_tag="PZ377_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDM6357449.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            12930..13025
                     /locus_tag="PZ377_000559"
     CDS             12930..13025
                     /locus_tag="PZ377_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357450.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(13088..13402)
                     /gene="trxA"
                     /locus_tag="PZ377_000560"
     CDS             complement(13088..13402)
                     /gene="trxA"
                     /locus_tag="PZ377_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDM6357451.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(13575..15923)
                     /locus_tag="PZ377_000561"
     CDS             complement(13575..15923)
                     /locus_tag="PZ377_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDM6357452.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(15933..17645)
                     /gene="polX"
                     /locus_tag="PZ377_000562"
     CDS             complement(15933..17645)
                     /gene="polX"
                     /locus_tag="PZ377_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDM6357453.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(17718..18239)
                     /locus_tag="PZ377_000563"
     CDS             complement(17718..18239)
                     /locus_tag="PZ377_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDM6357454.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(18240..18506)
                     /gene="zapA"
                     /locus_tag="PZ377_000564"
     CDS             complement(18240..18506)
                     /gene="zapA"
                     /locus_tag="PZ377_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDM6357455.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            18876..19814
                     /locus_tag="PZ377_000565"
     CDS             18876..19814
                     /locus_tag="PZ377_000565"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDM6357456.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(19985..22387)
                     /locus_tag="PZ377_000566"
     CDS             complement(19985..22387)
                     /locus_tag="PZ377_000566"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDM6357457.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(22387..23445)
                     /gene="pheS"
                     /locus_tag="PZ377_000567"
     CDS             complement(22387..23445)
                     /gene="pheS"
                     /locus_tag="PZ377_000567"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDM6357458.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(23826..24566)
                     /locus_tag="PZ377_000568"
     CDS             complement(23826..24566)
                     /locus_tag="PZ377_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDM6357459.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(24806..24913)
                     /locus_tag="PZ377_000569"
     CDS             complement(24806..24913)
                     /locus_tag="PZ377_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357460.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(24950..25273)
                     /gene="isdG"
                     /locus_tag="PZ377_000570"
     CDS             complement(24950..25273)
                     /gene="isdG"
                     /locus_tag="PZ377_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDM6357461.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(25292..26026)
                     /gene="srtB"
                     /locus_tag="PZ377_000571"
     CDS             complement(25292..26026)
                     /gene="srtB"
                     /locus_tag="PZ377_000571"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDM6357462.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(26088..27053)
                     /gene="isdF"
                     /locus_tag="PZ377_000572"
     CDS             complement(26088..27053)
                     /gene="isdF"
                     /locus_tag="PZ377_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDM6357463.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(27066..27944)
                     /gene="isdE"
                     /locus_tag="PZ377_000573"
     CDS             complement(27066..27944)
                     /gene="isdE"
                     /locus_tag="PZ377_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDM6357464.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPDVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(27941..29017)
                     /gene="isdD"
                     /locus_tag="PZ377_000574"
     CDS             complement(27941..29017)
                     /gene="isdD"
                     /locus_tag="PZ377_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDM6357465.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
BASE COUNT         9170 a         5317 c         3922 g        10658 t
ORIGIN      
        1 ggattaatta tagtcccatt attaacaaca aatgatgcgg attttgtatg gacttcttca
       61 tcattaatat atatgattct tcttacgtcg attatcctaa tagtttatat taaaactaaa
      121 gatagcttac agttaacgat atttatactt aatatagcct taattattct atactgtgca
      181 ccattactat tcatttatgt attctagtgc ttcattaata ttttaatgca tcagtttaag
      241 taaattccat ttggaaaata aaaagcaaat atcatcatca aattaacata tttaacgtta
      301 tactttcata aaatttgaaa caattgaaca tccttacttt agctttatat ctttatttca
      361 agtcatgttt tcaactattt taatgaatta aacatatatt ataaggagtc aacatcatga
      421 ataaacatat aaataaaaaa gccgcaaaac tacttgaaga ttataaaaag gttcaacaaa
      481 gaaaatcgaa agaaactgat agaggttgtt tgaaaagtgt catcatgctt atcgtcttat
      541 ttttcttaat tatttttggt attgtggcat gttctacaaa cgtaaacttt ttcttccagt
      601 aaaagagtga gatagaaatg ataaagaacc actaatgatt tattatgtag tggttcttta
      661 tcattagctg tagctaatgt gtacttaaaa aatgaataca tgagtaaagc tcatgcataa
      721 gaaatactaa ttgctaaaga aaaagtattt ctttatcgat gtcctacccc aacttgcatt
      781 gtctgtagaa attggggttc caatttctct atgttggggc cccgccaact tgcacatttt
      841 tgtaagctga cttttcgcca gcttctatat tggggccccc gccaacttgc attgtttgtt
      901 ggaattggtg atcctatttc tctctgttgt ggccccacga gtattgaaaa aacttgttat
      961 aagcttattt tcatttagtc aactactacc aatatagcac tagaactgga aatgataatt
     1021 taatattgta cacttttgat tgtttaaaca tgtagatatt ttaaaaaata ggagagcaaa
     1081 gtaaataatt gatatagtta ttttgagagt aatcctagga actattgtat ttatatttgt
     1141 ctcccctact tttaaatgtc attcattata cataagcatt ttgatataga atttatcaca
     1201 tatgcaaatt gaaaacaggt taagaccatt ttttgtctca acctgtttta tttattatct
     1261 atttataatt tcatcaattt ctttgtatat tttttctaat gcaactttag catcagccat
     1321 tgatacgaaa tcatttttct taagtgccgc tttagctcta tattcattca tcataatcgt
     1381 acgtttataa tatggattta agcttcctgc agctaatgca tatacattcg atttgttaag
     1441 taaagttctt aattcttctg ctatgcgttt atcatgaaac tgactttcag ttaaatattt
     1501 gtccagacta cttgcatctg ctttattccc ttctatagtt gctatcccag ttgatgataa
     1561 aagtaatgct aatgttcctg ttaatatata ttttttaaat ttcattataa gctctcctta
     1621 aaattgatac ttactgagta tatagtcaat ttcaggacaa ttacattaac ctgtcattaa
     1681 atagattact tttttcatta acaaaaatta acataacatt taattaatta tttccagata
     1741 acagcaacga ttgaagtttt atttaactaa tccttgtttt aatacattat caattcgctc
     1801 ttgtaagacc attttcttca ctttgtattc aaacgaaact aagttgactg ccttttgtgc
     1861 ttttctgtgt gcactgacag tatgtgtttt ttcaaattct ctcacaagat tttgagcttg
     1921 aatcaatttt tgttcttttt taatagttgc atcagttttc gctgctggtt tattaaattg
     1981 tggacgtgca ccatattcga atgtaccatc gttaaattct aaaatattat agtcatgttt
     2041 gaatttaata tatttaggtg ttgagttaaa ttttggtcta gattgatatt taaaagtacc
     2101 atcattgtac tctacgatat tgtgattaat actcactggt ttcttttctc ttggaccgta
     2161 tccttcgctc gcatctgctg ttgacgcaat tgtagttgta gtaataccta ttgccgctaa
     2221 tgttaataaa gattttgcta tcaatttatt tttcatgtta attatcctcc aaattattat
     2281 cttacattta ttaagttatc ctatttatac aacttttgaa attaactaat tattaattaa
     2341 ctataaacac tttataatat agacaaaata tagctatcta tcttgtgact catcagcact
     2401 tatacactag tcaaaaaatt catgcctcta ctttttcgaa tttcaaaaat aaaaccacac
     2461 aacaaaaaag acaccatttg aatgttggtg tctgaaggta taatctcata gattttattt
     2521 gtaacttctc attctatagt taaatattgt gacccagcta atgattaacg ttggtagaat
     2581 tactaaattc aaattagaca cgtcaaaaaa gacagattgg aaattgtcga tattaatatc
     2641 attaatgtcc attttttcat ttactgcttg atagatcgca aagtaaacta agaaccattg
     2701 aattaaatgc tctaaaacga caacctctgt caaatcgaca ttcttttttc taaaaagtaa
     2761 tctaactata aataattgga atatacttga aatcaagatc aatataatac ccattaagaa
     2821 aaaactacca aaatcaattg tgataaattg aataatacca taaataatta cggcagatat
     2881 tgccagtgaa atatatccta taaagataaa taccacaccc ataaagagtg tctttaagaa
     2941 cagaaaaggc ttctttctat ttgtaaccaa atagctcaat atacaaagta gtaatagaat
     3001 tgttaaaatt ttaattatca tcgtttgacc tcaatatatt tatttatatc tttattattg
     3061 accacgaaaa ataacaatca cttaagtgat tgccaataat actcaatgcc tactctatag
     3121 taatcttggc acgtgatttt acactatttt atctatttaa aaatgtaagt gttgaattat
     3181 gtttacttcg aaaatgcata attcaattaa atagtataat ttttaactta tatattaatc
     3241 ataacatatc aaccataatt agaatatatc tcttatttct ttataagtaa aattatacat
     3301 attaattact tctctactat gaatcatttc tttgttttat aaaaaatata tatcaaaaca
     3361 gctatatgta taacaatttg gagtgtttgt ctatccaact tagcaaacaa aatgtagaga
     3421 aatgtaaata tcgttttagc aataaaaatt attcaatcat agttgagtga cgtttactac
     3481 tattattata ctatcaacaa attcaataat acctcaaata aaaataatac catcaaattc
     3541 atcattattc tttttcgatt gcattaaacc ttattaattt gcacttaatt ttaattaatg
     3601 aagtgttaac tggtaaatgt atgaaaccgt ttgtaaaata gcagtgtact cgcattagta
     3661 agtacaaatt tattttacag ggagaactta ttatgaaaaa aaatatcaca aaaactatta
     3721 tcgcatctac agtaatcgct gcaggtcttt taactcaaac taatgatgct aaagctttct
     3781 ttagttatga atggaaaggt ttagaaatcg caaaaaattt agcagatcaa gctaaaaagg
     3841 acgatgaacg tattgataag ttaatgaaag agtctgataa aaatctaact ccttacaaag
     3901 ctgaaactgt taatgatctg taccttattg ttaaaaaatt aagccaaggt gatgtgaaga
     3961 aagcagttgt cagaattaaa gatggtggtc ctagagatta ctatactttt gacttaactc
     4021 gtcctttaga agagaacaga aaaaatatta aagttgttaa aaacggtgaa atcgactcga
     4081 ttacttggta ttaaaaaaca tactgaatta aatagttgta cgccaaacgt tagaaaacaa
     4141 tgctaacgta ttggcgtgct tttttatttt aagtaacttc caatttattt agcatcttta
     4201 cgactgttta ataaagcacg tatgattaac acggttgcca ctatatccgt tacaattttt
     4261 atgattgtta atacattcga tttccttttc acaatagcca cctcgcttgt tcaaaacata
     4321 aacaactatt gcattcactt ttaagtaatt atttatatta tttatcctaa taagctcacc
     4381 attcaaataa acacaatatt ataaaataat tatacctttg gatatagcaa aaagccacac
     4441 tctatagcat ggcttctatc aattatttta aagtattata ttttaaaact agatcgattt
     4501 gtctttgtaa ttttttcttt tcataactgt gttggaaatg aattaaatta acagctcttt
     4561 gtgctttacg gtgtgttgca acagttcttg tacgtttgaa aaagtttaca gctttttgtg
     4621 catccacaac ttttttattt acttgttttt taaagtttgt ttgatactga tcatattttt
     4681 tagcagcttc aacgttttta gtttgtgcat gagattcacc ggcaaacgtt gataccgtta
     4741 aactaatagc agctatgctt aaaattgatt tcccaataaa attctttttc ataattaata
     4801 acacctatcc tcaaatattt attttgtata tatttataat aatagacaga tacaaaaaac
     4861 tacattaact atttattaac taaacattaa tatttgtgtc actacatttc tgaatttcaa
     4921 gaattttgct actccatatt ttaaccatca aattattgat ataatttatg actttagaaa
     4981 tccttttaaa tatgtatacg attcatgatt ttaactaaaa tttcaaattt cctgacttct
     5041 tggaatctta attttatact ttttaataat acacttttaa ccaaataatc aacatgattc
     5101 aaccatatag ggttatcaac aatcacattc tccaaaatta caattctttt cataacataa
     5161 atgtgttatt cattccaatt atcttataaa attgtcagaa atacttttaa acaaacattc
     5221 atgacacgac ctcctagtta aatcagtcta taaactaaaa aaagccatag ctacttctat
     5281 ttaaaaagta gtatgactga ttgtttcgtg atgtcgattt ttgaaattga tggtgccatg
     5341 taaagtttga tcatcaataa taacttcagc atatgtttct tccattgaac ttctagattg
     5401 agatatactt ccaggattaa taacatgaac accattaata tactcatatt ttgccacatg
     5461 tgtatgtcca taaaatgcaa acaaacaacc taattcaagt cctttttcag ctaataaatc
     5521 tcttgttcga ttgacttgat ataaatgtcc atgagtataa aatgctttca cgtcattata
     5581 ttttgcgacc gcttcatttt caaattctgg gtaaaaatca caattgcctt ttactctatt
     5641 aaataagcta agttccgtat catcatacgc gaattctgaa tctcctaaat gtaaatatac
     5701 atctgcatct gggtgcattt cataaatttg atataaaacg cctgattcag tatggttatc
     5761 actcacaata atccatttag acatttttat caccttcaag aaaagcttga agtaaattaa
     5821 tcgcatttcg tctatgacta atttgccctt tttgttcttt tgaaagttga gccatggttt
     5881 tatctaattt cggtacataa aatatcggat catatccgaa accattttcg ccatattttc
     5941 catctgcaat ttcacctgaa acagtacctt taaatacttt tgtttccata tcagggccac
     6001 tcatacttat gacacaaaca aattgcgcac gacgatcagt tgtattacca agcttattta
     6061 ataatttttc aatatttgct tcatcgcttt tattttcacc agcataacgt gcagagtata
     6121 tacctggctc accatttaat gcaaaaactt ctagtccact gtcatcagct atgaccgttt
     6181 tattcaatgc ttttgcagca gcttctgatt ttaatatagc attttcttca aatgttgatc
     6241 ctgtttcttc cacatcaaaa tctggtatta gttctgaaat acctattacg tggtaatctg
     6301 gaaatattac tttaaagtca tttattttcc cttgattatt cgatgcaata acaatctctt
     6361 tcattaaaaa tcctcctagt cattcactga tatacgttcc acattgacag ataaatttaa
     6421 ccactctttg ataatgttag taatgtgagt aggatcacct gttgcaaaaa atcgatgatc
     6481 tggatgttca gtataacttg catgttcatt actaaatgtt agcaatgcac taacttcacg
     6541 agccgtttct aatccagacg aaatcactgt ctttttacca ccaaaataat catagatagg
     6601 tttatagagc aatggatagt gggtacatcc taaaatgaca gtatcagact cactattacg
     6661 ccaacgtttc agtgtttgat gaatgacaat gcttgtaatt gttggatcac tatatctcat
     6721 ttgttctaca agtggcacaa aacctggaca ggcaacgcca tgtacctcta catgtggatt
     6781 gatacgttta atatgcgtac gatatgcttc agatttaatt gtgccttccg ttcctagtac
     6841 taatacattt tgatttctag tagtcattat tgctgttcta gcacctggtt caattacgcc
     6901 aatcactggg attgataagg tcttttgtaa atattctaaa gctacagcag ttgctgtatt
     6961 acaagcaatc acgagcattt ttatatcaaa ttccattaat ttacgagcga tttcaactgt
     7021 atattgtttt acttgttctc ctggtcttgg cccatatgga catcgtccaa tatcacctaa
     7081 gtaataaatc gtctcatttg gcaactgacg cataatttct ttagctactg tcaaacctcc
     7141 gacaccagag tctattacac ctattggttt attcatattc ggtcatcctt acttttaatt
     7201 taatttttag tttatcataa ctaagcattg gattttagta ttatgcactg tgtttaccat
     7261 ttttgtcatt ataatattta ttttaaatca gcccactatc atattgtcat gtaatctgct
     7321 tattaaaaaa atcccttcca agttattgtg tatctccatt caatttaatt ttgaaaggaa
     7381 cataacattt taactcaaaa gggattaatt tttattctac ttcatggtct gaaccaaaga
     7441 atgatttaaa catgtggaat gttgtttctc tgttcattgc tgcaatggat gttgttaatg
     7501 gaataccttt agggcaagca ttaacacagt tttgtgaatt accacactgc tgtaagccac
     7561 cagtacccat taatgcattt aaacgttcat ctttagtcat agatcctgtt gggtgcaaat
     7621 taaacaaacg aacttgcgag attgcttgtg caccaacgaa tttattattt tcagtaacat
     7681 taggacaaac ctctaaacat acaccacatg tcatacattt agataattca taagctgttt
     7741 gacgtttttt ctctggcata cgtggtcccg gacctaaatc atacgttcca tcaattggga
     7801 tccatgcttt catacgtttt aagttatcga acattctaga acgatcaact tgtaagtcac
     7861 ggataactgg gaaagtattc attggctcta aacgaatagg ttgttctaat tgatcaacaa
     7921 tcgcagaaca agattgtctt gcacgaccat tgataaccat agaacatgct ccacatactt
     7981 cttctaagca gttcatatcc cagacaacag gtgttgtttt ttcaccttta atattaactg
     8041 ggttacgtct aatttccatt aaacaagcaa tgacgtttaa attttcacga tatggaattt
     8101 caaatgtttc ttcataaggc ttagaatcac ttgtatcttg tcgtttaata attaatttta
     8161 ctgttttttg tttcggttta gattgtgttt catgttgtgg agtgtttttc actgattgtt
     8221 cagtcattat tttttacccc ctttagactt acttgtgtaa tcacgtttac gaggtggtat
     8281 taaactcaca tcgacgtcat cataagtaaa ctgcggtttt tcaaatgcgc cttggaatga
     8341 ggccattgtc gtttttaacc actcttcatc attacgctct gggaattctg gtttataatg
     8401 ggcaccgcgt gattcgttac ggttatatgc accaatcgta ataacacgtg caagtactaa
     8461 catgttccat agttgacggg taaagaatac cgcttggtta ctccaagttt gagtatcttc
     8521 catatcaata tcttcataac gtttcatcaa ttcaacaatc tttttatctg tttctaacag
     8581 tttttcattt tcacgaacaa cagttacatt tgctgtcata atttcaccaa gttcacggtg
     8641 taatttatat gcattttctg taccgcgcat agctaataat ttatcaaaac gttcttgctc
     8701 ttcagcttta cgcttttcaa aaatactttc gtccatatca gtatatgatc gatcaatatt
     8761 tgaaatataa tcaatcgcgt ttggacctgc tactgtacca ccataaatcg ctgataacaa
     8821 tgaattggca cctaagcggt taccaccatg ttgagagaag tcacattctc cagctgcaaa
     8881 taacccttta atatttgtca tttgatcata atctacatat agaccaccca ttgaatagtg
     8941 aacagctggg aaaatcttca ttggtacttt gcgtgggtca tcaccagtga atttttcata
     9001 aatctcaatg ataccaccta gttttacatc taactcatgt ggatctttat gtgacaaatc
     9061 aagatatacc atgttttcgc catttatacc taatttttgg ttaatacata catcgaaaat
     9121 ttcacgcgtt gcgatatcac gaggtactaa gttaccataa tcaggatatt tctcttctaa
     9181 gaagtaccaa ggcttaccat ctttatatgt ccaaattcgt ccaccttcac cacgtgctga
     9241 ttcactcatt agtcgcagtt tatcatcacc agggattgca gtaggatgaa tttgaatgaa
     9301 ctcaccatta gcataaatag cgccttgttg gtaaacaatg gaagccgctg atcctgtatt
     9361 aatcattgag tttgttgttt taccgaaaat aataccaggg ccacccgttg ccataataac
     9421 tgcatctgaa ccaaatgttt caatctcagc agttgtcata ttttgtgcaa cgatacctct
     9481 tgcactatca tcgtcacctt taactatgcc aaggaattcc catccttcat acttcgtaac
     9541 taatccatct acttcatatg cacgaacttg ttcatccaat gcatataata attgttgtcc
     9601 agttgttgcc cctgcatatg ctgttctgtg atgtaatgta ccaccgaaac gtctaaaatc
     9661 taatagacct tcatttgttc tattgaacat tacgcccata cggtctaata aatgaataat
     9721 tttaggtgct gcctctgtca tcgctttaac aggtggttgg tttgcaagga aatcgccacc
     9781 atacactgta tcatcaaagt gaatccaagg agaatcgcct tcccctttag tattgaccgc
     9841 accattaatg ccaccttggg cacaaacaga gtgcgaacgc tttactggta caactgagaa
     9901 caaatctaca tgtgcacctt tttctgccgc tttaattgtt gacattaagc ccgctaggcc
     9961 acctccgaca acaataagat gtttctctgc cataaaaatt tcactcccct aaattttcaa
    10021 tctatatttg ttaaatgcga tgtattacat aaaggcaata attgcagtaa caccaatata
    10081 cgaaataact aaaaatacga ttaatgaaac ccatgtaaat actcgttgtg attttggaga
    10141 ttgaagtcca ccccaagtaa ctaagaatga ccataagcca tttgcaaagt ggaacacaac
    10201 agcaataata caaataatat aaaatattgc ccatccagga tgttgcaatg tttcgtgcat
    10261 taaatcgtaa ttcacttctt tgccgtaaaa tgctttttgt aaacgtgttt gccataaatg
    10321 gataccaata aagataaatg ttaagatacc actcactctt tggaagaaga acatccagtt
    10381 tctaaaaatc gagtaatgtc caacattttc ttttgctgta aatgcaatgt gtataccaaa
    10441 caaaccgtga tataacaacg gaatgtatat aaataaaaat tctacaataa ttagaaatgg
    10501 taatgattcc ataaagttag atgccttatt aaacgcttca gcaccttgtg ttgcttggtg
    10561 attcactaat aaatgaacga ccaaaaatgc acctattggg ataataccta ataacgagtg
    10621 aatacgtctt agataaaatt cattttttga ttgagccaaa aggagtcccc cctgtgaacg
    10681 aatatttaat ttattgagct atttatatta aacgtacgct taacccccta aagtgatatc
    10741 gtttctagcg ttgtcattat cattaagcga aacattttaa agacaaatac acactgcacg
    10801 atcaccaaac tgcatgtcga acaatgtaac atttggattc gatatttaaa attgcttgtg
    10861 atgataaact ttctcattta gaaaacgctt ccacgtacat tcaaaaaaat aactttgtta
    10921 accatattgt aacattattt catatatttt ggggcatgag aatgattctc acgcccagta
    10981 atttatttat gcaattgttc atgtaggttc tttgcgacgt tttcaggaat acctatattt
    11041 ttaaaatctt caagtgtagc ttccttcatt ttcttgattg aaccgaatga acgcaataat
    11101 aatgttttac gtttgttacc gataccatct atatcatcaa gtattgattt caagcctgtc
    11161 ttttgacgtg tttgtctatg aaatgtgatt gcgaatctgt gaacctcatc ttggatacgg
    11221 tgcaacaaat aaaatgcctg gctatttttc ttcagtggta caatttctgc actagcgcca
    11281 tataataatt cagatgtttg gtgtttatca tttttctgca aacctgcaac agggatatca
    11341 agacctaatt cgttttgtag cacatcaata accccgttca tatgtccttt accaccatct
    11401 actattatta aatcaggtaa tggtaatcct tcgtttaaaa cgcgagaata tcgtcgtctt
    11461 actacttctc tcattgattt gtaatcatct ggacctttaa ccgttttgat tttatacttt
    11521 ctataatttt tcttatctgg tttaccgtcg acaaatgtaa ccattgctga cactggatcc
    11581 acaccttgaa tattagaatt atcgaatgct tcaattctaa ttggtgtttg aattcccatt
    11641 tgtgttccaa gttcttcaat agctttaatc gttctggact catcacgtga tattaattca
    11701 aatttattat ttaaggatac tttagcgtta tgtgcagcta ggtcaaccat atcttttttg
    11761 ggacctcgcg cgggttgaac gattttagtg tccacaacag attgaatcat ttctttatcc
    11821 aaattacgtg gtacatgaac ttccttaggt aaaatatgtt ggtttaagct ataaaattgt
    11881 ccaataaatg tataaaattc ttcttcttct gtttgctgta atggaatcat cgttgtatct
    11941 cgctttatca tattaccttg tcgtataaag aaaacttgga tacacatcca tcctttatca
    12001 acactataac caaagacatc acgaatcgtt ttatctgatg acataatttt ttgtttgttt
    12061 gtcagatttt gaatatgttg aattaaatct ctatattctt tagcccgttc aaaatcaagt
    12121 gattcacttg cagttaacat tcgctcttct aaacttttta aaattgtttt gtcttcccca
    12181 ttcagaaaat cagtaatttc cttcgtcatt tgtgcgtatt tactcaaatc aacgtcatat
    12241 acacatggtc ctaaacattg tccaatatgg taataaagac ataatttatc tggcatctta
    12301 tcacatttgc gatatggata tattctgtct aataactttt tagtttcttg agcagaatat
    12361 gcattcggat acggtccgaa atatttgcca gtaccttgtt ttacagttct cgtcactagt
    12421 agtctaggat atttctcctt cgtaatttta ataaatggat aacttttatc atcctttaat
    12481 aatatattat atcttggttg atattgttta atcagattca attccagtaa aagtgattct
    12541 gtttcacttg acgtgacaat aaactcaaag cgacgtattt caccaaccag tctcgttgtt
    12601 ttagcatcat gagcacccgt aaaatatgat cgcaatcgat ttcttagctt tttagctttg
    12661 ccaacatata tcacttgatc attacgatct ttcattaaat agcatcctgg ttccataggt
    12721 acgacattta atttattttt aattcgttgc ttatagtctt ccaaaaatat ccccccttct
    12781 aattttaaaa tgaatgatgc tacaaaagtg cttcaaattt atcaccacta attctagtta
    12841 ataatcaaca tcttcgagta tgacttttca tgcacatcat gtaatattga acatggtttt
    12901 atcaaaccaa ctgctacaaa tctatcagca tgctagatta tcatttaacg ttcatagatt
    12961 tattaatccc accattctca agtcgtaaac aatatgatga cttatcattc aactttttat
    13021 attaaaaaca caaacaaaaa ctggagcaat tcattaaaga aatgccccag tcgtcattgg
    13081 ttgtaactta taaatgttta tctaaaactt cagctaagtt ttcttttggt tggaaaccaa
    13141 caactttatc aactggttga ccgtctttaa agacgattaa tgttggaata ctcatcactt
    13201 catatttagc tgcagttgat ggattttcat caacatctaa ttttaaaatg tcagctttac
    13261 cttcatagtc agctgctaat tcttctaata ccggagcgat cattttacat ggaccacacc
    13321 atgttgccca aaaatctact agttgtacac cagattctac ttttgaatca aaatctgcat
    13381 ctgttacttt tacgattgcc ataaatgcca atcctcctta attattatta tgatgttcaa
    13441 attatatcag ataacatgct tcatgtattt ctatttgctc aatttttatg cttatttcaa
    13501 ataacatgtt tgaaatgtat tcttgttgct ttacattatt ttaactgcag ctatttaatt
    13561 tatcaaatta taatttattt tagtgttgca acggtaacgc caaatccacc ttcgcttggc
    13621 ataccacctc taaagtcact aacactttta tgctttttca aatgttgttg tacacctttt
    13681 tgaagtgcac ctgtaccttt accatgaatg atataaactt gttcgtaatt acttaaaacg
    13741 gcttgatcta aatattgatc tagttcaatt aaagcatcct cataacgata gcctcgtaag
    13801 tcaagttcag ttttaattgt ttgacgattt tgacgtgtaa ccattttcgt tggcttaact
    13861 ttttcttttt gttttttctc taaatcttca ataggtaact tcattttaat aattcccatt
    13921 tgaacaattg cttcttcatc attgacaatt tctaaaactt cacccttttg accgtaagat
    13981 aatactttta cttcatcacc agcaacaatt ttatcgtatt tttgcttttg tacattttgc
    14041 tttatagatt tcgcttcata atgatcatct aatcgtttct tcttatcaat caattcatgt
    14101 tctttaacat ctgcaccttt ttgttcacgc aattgtctta agtctttaat aatatcgtca
    14161 gcttcttttg ttgcagcctt aatcttctga tttgcttttt ctttcgcttc ctctattaga
    14221 gacttttcat aattttggaa ttgttgatac tgcttagata aatcatcatg cacttgctcc
    14281 gcttctttta caagacggtc cagttctaac ctctgtgtct ctacacgttt gtaattacgc
    14341 tctaatgatt caatcatttc atttatttct ttttcatcag taccaatcat cgtcttagcc
    14401 ttattaataa tattcaaact aagacctaac tttttagaaa tgtcaaaagc atttgaacga
    14461 cccggcacac ccattaataa cttatacgtt ggactcaaag tatctacatc aaattctaca
    14521 ctcgcattca taacgccttc tcgattataa ctatatgctt taagttcagg atagtgcgtc
    14581 gttgccatta ctagagaacc aatttttcta acatgatcta aaatgctcat tgctaatgca
    14641 gcaccttcac tcggatctgt acctgcacct aattcatcaa ataaaactaa actatgtttg
    14701 tctgcatgct ttaaaatttc aactatattc gtcatatgag atgaaaaagt tgataatgat
    14761 tgttctattg attgttcatc tccgatatcg caatatacat ttttaaatac acttaactga
    14821 ctaccatcaa gtgtgggaat caacaatcct gattgagcca taacaataat taaacctaat
    14881 gtttttaatg ttacagtttt accacctgta ttcggtcctg taataattac cgtttcaata
    14941 tcttccataa attcgatggt attagctaca acagtctcac gatttaataa tggatggtat
    15001 gctttaggta aatatacagt acggtcctct ttaaatatcg gctttgttcc tttaatactt
    15061 ctactatatc tcgcttttgc gattaaaaaa tctaactgac ccatgacttg ttctgccaca
    15121 agtagtgcat ctttgtccgc agccacataa ccagttagtt gcgttaaaat gcgttctttt
    15181 tcaattgctt cgtcatgacg taatcgacta atttgattat tcatttcaac aactgatgat
    15241 ggctcaatat acaatgtttg tcctgaagca gattgatcat gtacaatccc attaaaatct
    15301 tgtcgatatt cagctttgac aggtataacg tttctttcat tcctaactgt tacaatagca
    15361 tctgataatt ttttctgatt tgcttggctt ttaacaatac ggtccaaatt ttgtctaata
    15421 cgttgattcg tgctagaaat tttacttcta atcccttgca attcataact cgcattatca
    15481 tataaatcat acgtatcgca tgtttcattt atttgttgaa aaagatcagt taacacaggt
    15541 aattgattca tcttgtcatc taatattggg tatttaacac cttcatcttc ttcaaccaat
    15601 tgattataaa atgtcttgaa ttgattttgt acttgaatta atctttttat caagttaagc
    15661 tctgatacat ttaaaacgcc gccaatatca gcgcgatgaa tgaatgctga tactttagat
    15721 aagccactca agcttggtaa acgatgctta ttatagattt gagcaatctc atccgtttct
    15781 tccatttgaa aaacaaccgt ttcaaaatta gtagctggca tcatttgatt gaccttttcc
    15841 aagcctaagt cactaatagt ttcattggca acgagtgatt ttattttttc aaattctaag
    15901 acgtctaatg ttttttgtct cataaaatcc ctctatttct taagttttgt attattttca
    15961 ataaagtctt taaaagcttc acgcgataat gcgttaatca ctctatcttt tgttacaaat
    16021 cctttttgcg cagttgctac gccataattc ataaaatcta aatgatttgt atgatgcgca
    16081 tcagtgttaa tagttaattt cacatttgga tatttacgaa cgatatcagc gctcagatcc
    16141 agtcgatgtg gattggcatt aatttctaat actgtattcg tttcttcagc taatgccatt
    16201 aattgttcaa tattcggttt ataaccatct cttctaccta taatacgccc tgttggatgc
    16261 gctatatgtc gcacgtatgg attgcgacat gcattagcta atcgttccat aatttgttct
    16321 tctgattggt taaagctttg atgaatagct ccaattacat aatcaagttg tgctaaaatt
    16381 tcatcatcat aatccagcga gccatcaggt aatatatcca tttctgtacc tgaataaata
    16441 tcaatttcac tatattcttt atctaaagcc ttaatttctt cgttttgtct taaaagtctt
    16501 tctacttgta agccattagc aacacgtaaa ctttgtgaat gatcagtaat taccatgaat
    16561 ttataacctt ttgcgatatt tgcttctacc atgtctcgaa tagaaaacgc accatcacta
    16621 tacgttgtat gcatatgaat atcaccatta atatcatcta ttgtaatgat attacttaga
    16681 tctttatcaa attcgctacc atcttctcgc atagcaggtg gtataaaatt cacattaaaa
    16741 tgttcatata tcttggcttc actatcatat tgaattaatg taccatcagc ttgttcaatt
    16801 ccatattcac ttactttttc atcacgtgct ttagcaagtt gtcgaattct tatattatgt
    16861 tcttttgacc cagtaaaatg ctgcaatgta tgataaaaag cacttggttc aattaatcga
    16921 aaatcgacac caatcgtttc atcatcatac gctaattcta atgaaacttt tgtgttcccc
    16981 actgcaactt cttttacttt attgggaata tttaataatt gctgctgcac tgcttttggg
    17041 ttatcggtac ttattatgaa atctaaatct ttgctcattt ctttaaaacg acggaagctt
    17101 cctgcagatg aatattgatc gatataattt aatgtatcta tataatcaat gatttcttga
    17161 ttaagtcttc tcatttgatc aattggatat ctatctttct tagcaccaag ttgtttcaca
    17221 gcttctaata tgttttgttc cgttttctta gcaaatccgc ttaattcact aacttttcca
    17281 ttttcacaag caacttgaag tgacgcttta tcaacaatat tcaactcttt atatagctta
    17341 gcaattttct tgcttccaag tccttgaatt ttcaaaagtg gaataagacc ttccggaact
    17401 tcttcctgta attgctgtaa atactgagat tcaccggtct cacggtaatc attgattact
    17461 tctgcaacac ctttaccaat gccttttaac tccgttacat cagatatttc atctaatggt
    17521 cgttcatcta attcaagact ttgagctgct tttcgatacg ctgatatttt aaaagtattt
    17581 tcccctttta attccatata agtagcaatt tgttctaata gtttgataac atcttttttt
    17641 gtcataataa cactccataa aaagaagacc aggacgtatc attaatatat acctttgtcc
    17701 tgacctctta tgataattta taaattaatg gtgaaagccg ataaatatgg gatgtgaaaa
    17761 atcaatgaat gactcacaat agatatttta agctgatgtt gtaatgcttc gttaggatat
    17821 aatgccaata gatataacgt aaagtgtaag acaattatcg tcataaacac accaactatc
    17881 attcccattg cacgactgaa aatatgaatg ttttgatacg ctattatttt atcaaaagtt
    17941 acgataatta gatataaaat gaacttacaa aacaatgtaa tcattaaaaa agctacaatc
    18001 gcttcaaatc gattttgtag atgattaaaa tgaaacgcaa aagttgtatt aaatgctgtt
    18061 gttttaggat atggaataaa tacaattaat ctttctacaa tagatttgta aaattgactg
    18121 gcaatccaca atgatacaat cgttgcactc aaatgtatca tagataacca aaaacctcgt
    18181 ctgaatccaa cgatgacaaa atacacaaag aaaatgatta tgataaaatc aatgaccatt
    18241 tattgctcac gctgctgcaa tttgtgaatt tgttgtttca aacgtcgatt ttcttcttct
    18301 agtagtactt tttcatgcat aatattcaca gcagttagta ttgcttttct tgaagtatct
    18361 aaacctgctg ctttataccc taattctttt attttatcat caactaaatg tgctacatat
    18421 cgtatgtgct ctgggttatc ttccccaaca attgtaaaaa gctgatcatt aattgataca
    18481 tttaccttgt ttttaaactg tgtcatttat aatttctcct gatccttttt ttaaaatcta
    18541 aattcacgtt ataaaatatg actggatagt ttgtctgaat ttgatactaa tattgttata
    18601 ttgcaattat gataaaacaa caacacaatc tctatagatg acttaatgtt ctttttataa
    18661 tgaaataatg taaagaattt tctattcaat actttatcat gtttaaattg tgtcactata
    18721 acattttcat aaacattata catgaccact atgtattttg taagtatccg caattaattc
    18781 tttacaacat acataaatgt ttctgacgtt attatcattt atgatatgat tatttttgct
    18841 aaagacaatg aaattttatg aaaggattta cacaaatggc gaatatcgtt tttaaattgt
    18901 cggataaaga cataacgaca ttaatgtcac gcatttcttt tgatactgag aatttacctc
    18961 aaggtatgaa agcacgtgca aagtatcaaa atacaactgt aaatatttac caatcaggta
    19021 aagtcatgtt tcaaggtaat catgcagaag ctgtgtctga agaacttcta ccgcagcatt
    19081 ctcaattgaa tactaataaa actaaaaaga aaaatatggc taattctttt cttgaacaaa
    19141 ctttaatgta tgatcaattt aactgtattg gtagtgatga ggctggaagt ggcgattact
    19201 ttggtccgtt gactgtgtgt gctgctttcg ttaccaaaga gcatgtccct attttaaaaa
    19261 ctttgggtgt agatgattca aaaaaattaa ccgataccaa aattgtagaa cttgctgagc
    19321 aattggttac tttcatacca cattcattat taacactaca caatgaaaaa tataatattc
    19381 agcaagcaaa aggctggact caagtaaaaa tgaaagctgc ccttcataat gaagccatca
    19441 aaaatgtact tgaaaaaatt gattcgtctc aattagacta tatcgtcatt gatcagttcg
    19501 caaaacgcga agtgtatagc cattacgcat tatcagatat accacttcca aagaaaacaa
    19561 aatttgaaac aaaaggagaa tcgaagtcat tagccattgc agtggcaagc ataatttctc
    19621 gatacgcatt tgttacatat atggatcaaa tatcaaaaaa tattaatatg acgattccta
    19681 aaggtgccgg tgctaaagtc gatgtaatcg ccgccaaaat tataaaaaaa tacggtcttt
    19741 cacgtttaga taccatttca aaaaagcatt ttaaaaatcg tgaaaaagcc caaaaaattt
    19801 taaagcctct ttgattttta atcattagag gcttttatgc agttgtgcga agcatcatct
    19861 gtattccgct gttcctcttt gactttttaa atgcattgga accaaattgg tgaacccaaa
    19921 aataaaaccc catgttaggg atatcaatct acttcagata tccttataca tggggtttaa
    19981 atcattatct aataacagca ccttgttcaa ttaatgctgc ttcaatttcc gcttgtactt
    20041 ttgaaacgcg ctcatctgtc aatgtttctt ctgtgtctaa ataatttaaa cgtattgcaa
    20101 ttgatttttt acctttttct aaatgttcgc cctgatatac atcaaagaca agtgtatctt
    20161 ttaatatatt gccaccatgt gcatgaatcg ttgataataa atcagctgct ggaatatttt
    20221 gatctacttc taatgcaatg tcacgagaca tgcctgggaa tctcggaatt ggctggtaat
    20281 taatgtaacc taccgacaca gccattaatg catcaaaatt caactcaaaa acatacgtac
    20341 gttttaaatc attatcagct gctaatattg gatgtaattc accaataaaa ccaacaactt
    20401 tattctctaa taagatttca gcagtacgac ctggatgtaa tccatcaata tcagcacggc
    20461 gataactaaa ttcaagattt aacttttcag atactcgatc cacgacacct tttgctaaat
    20521 agaaatcaac cgtttctttc ttatcttgcc attgattgac tacataatct ccagttaaaa
    20581 taccacttaa atattcaact tgatctggta gttcaccttc tccattagca aagaagacat
    20641 tgccgatttc aaataatttt acatctttat ttttgcgtgc cacattatat gatgccgctt
    20701 cgattaaatg tggtaataaa ctttgacgta atgacgcatg cgcttcactc attggcatca
    20761 ataaatcaat tgtttgacgc tgttgcatcg aaaatgcagt agcatcttct ttagaaacta
    20821 acgaatacgt aatagcttgg tctaatccag caccttctaa cacttcttta accattctag
    20881 ttttatattg gcgatcagtt agctgaccac tagtaacttt atcgaagaca ggtaacgttg
    20941 atggaatatc gtcgtatcca tatatacgtg caacttcttc aattaaatct tctttaattg
    21001 taatatcttt acgacgtgat ggtactagca ctgtaataac atcatcattt atttctgtat
    21061 caaaccctag ttgattaaaa atagtaacaa tatcattttg tgacaaatca aatccaatag
    21121 tgcgattaat tttatcagca gtgatgtcga ttggtgtaat aaatgcacca agttctcctg
    21181 aagacactct atcttttagc acttttccgt ttgcataagt ttgtaataaa taacatgcac
    21241 ggtcgactgc ttcatctaca aattcagtag ctattccttt ttcaaaacga ctagatgatt
    21301 cactgcgtaa atttaaacgt cttgatgtat gacgaattga aactggatca aaaatagcac
    21361 cttcaatcac tatatttgat gtttgttctt taacttctga aaaatcgcca cccataacac
    21421 cagctaatgc aattggagtt tgtccattag taatgacaat atcgctcgtt aataattcac
    21481 gttctgtatc atctaatgtt gtcatttttt cgccttcatt agcttgacga acaacaattt
    21541 gttgtgaacc aatcgcatct tgatcaaaca tgtgcaatgg ttgaccgtat tctaataaca
    21601 cataatttga aatgtcaaca acattattaa taggacgtat acccgctttt attaagcgtg
    21661 cttgcatcca aattggcgag ggttcaattg tcacgtcgtg aacaacacgt gcactataat
    21721 atggtacttt atcttcattt tcaattgtca cagtcagttc atcatttgca gataaatcaa
    21781 gctcatttga tgttgtctct ggcttagtca tttttgtatt atataatgct gcaacttcat
    21841 aagcagtacc tatcatactt aaagcatctg cacgattcgg cgttaaatca aattccatta
    21901 cttgatcatc taaatataaa gcttgtaagg catctgttcc tggaacttgg gcttcactaa
    21961 atacataaat gcctgattca aaacttttcg gtatatagtt acttgaaata ccaatttctt
    22021 gtaacgaaca aatcatacct tctgaacgtt caccgcgtaa tttggcacgc ttaattttaa
    22081 taccaccagg caatctgcca cctactttag caacaatgac atattgtcct gcatcaacgt
    22141 tcggtgcacc acaaacgatt tgtacaggtt cgtcttctcc gatatcaact tggcaaacat
    22201 ttaatttatc agcatcagga tgtttctctt ttgacttaac gaagccgaca actaaatttt
    22261 tgatatcttt tgtgtagtca attaaatcat ccacttcaat acctgtgcgc gtaatacgtt
    22321 ctgccaaatt acttacagaa tcatcgattg ttacatattc tttcaaccat tcatttgata
    22381 tcaacattac atgtcacctc tatcttctac cgctttaaat tgatctaaaa atctcacatc
    22441 attagtatag aaatgacgaa tatcttctat accatatttc aacattgcaa tacggtctgg
    22501 tcccatacca aatgcaaatc cagagtactc tgaagaatca aaaccagcca tttctaatac
    22561 attaggatgt accattccag cacctaaaat ttcaatccat cctgtgtgtt tacacacatt
    22621 acaacctttt cctttacatt taaaacatga cacatcaact tctacagaag gttcagtgaa
    22681 tgggaagtaa cttggacgta aacgaatttc acgatcagca ccaaataatt tcttagctaa
    22741 caattctaaa gtacctttca aatcactcat tttaacgttt ttatcaacaa ctaatccttc
    22801 gatttgtgta aattgatgac tatgtgtcgc atcatcagag tcacgacgat acactttacc
    22861 agggcaaata attttaactg gaccttgacc atgacgtgat tccatcgtac gtgcctgcac
    22921 tggtgatgta tgcgtacgta ataaaatttc atccgtaata tagaaactat cttgcatatc
    22981 acgtgcaggg tgtgatttag gtaaattcag catttcgaag ttataatgat cttgttcaac
    23041 ttcatatcca ttcacaattt cataacctaa acctaagaat aagtcttcaa tttcttctat
    23101 tgtacgtgtt aatggatgct ttgaaccgat ttcaatatga cgacctggta atgatacatc
    23161 aattgtttct tcagccaatt gcttatttaa tttttcttta actaacatct gttgtctttc
    23221 atctaattca ttttgaattg tttgacgcaa ttcattcact ttttgaccaa acgcaggttt
    23281 atcttcattc ggcaaatcct tcatcaattt cattagtccg ctaactgacc cttttttacc
    23341 taagtatttc actttaactt cttgcagtgc acgttcatca tttgcttcat taatatctac
    23401 aagcgcttgt tgttttaact ctgacattgt ttgttgttca gacatatgtc actgacctcc
    23461 ctattcgaac tattcatttt ctatttatca tatttgttgt ttaactaaaa aaagactttc
    23521 atcctcttaa aaaataaggg acgaaagtca tttccgtggt accacccaaa tttatgccta
    23581 agcatacact tgtttaatat tgataacgga ttgtctttcc gacttaaatc tctttaagta
    23641 accaaaaagg tgaaaagact ttaaataatt ggaataactt gcagtcacga ttattctccc
    23701 taaaacaatt atttatataa atgtccatgc ctttttattg gtttatattc aattgtgtat
    23761 gcattcacgt agaaatgttc tgttaaacaa ttaattataa ttatatcgga aaactttcaa
    23821 cacggtcaac ctttcaaatg ataaagtaaa atactacctg caatcgctac atttaaactt
    23881 tcagctttac cataaatagg tatgattaaa ttttgtgtag tatgtgctaa taattcagga
    23941 ttaacacctt ctccctcatt acctaatagt aatgcaaaag aatcacttga agtaacttct
    24001 ttgtatgcca ctgcgttttc aagtgctgta ccataaacag gaccattaaa ttgagtaata
    24061 aacgtatcga gatcttgtgt cataactggc aaatggaaaa cactaccttg actcgctcgc
    24121 aacactttat cttgataagg atctgtcgta cccttctcca ttattacagc atccattcca
    24181 gcagcatccg cagttctaat taatgtgcct aaatttccag gatcttgaac acgatcgatg
    24241 agcaatactt gttgtgcttt agaaatatca taatgcggct tctcaatgat tgcgaaaaac
    24301 ccttgtggtg tcactgtacc tgataaagat tcagcaactt tcatgtttat ttcaaaaact
    24361 tcttgcgcgt atgcgataat ttgctgatct aatcttgccg gttcaattgc aaataattgt
    24421 gtaattacaa ttccactttg ataagcttct tcaattaaat gcacaccttc aattaaagct
    24481 aatccagttt tatccctctc acgtttcttt tttagcttgt tcgcttgttt aattctatta
    24541 ttttgtgcag aagtaatttg ttccattgat agctcctcgc tttattttta aaaataaaaa
    24601 tattaatcat taataagatg aaaacatttg attgtatagt taatattaat taatcgcttt
    24661 tatcactcat aatatttcaa attgtataaa tttcttttat cgatactact actataaatc
    24721 atacgcccca aaatatcatt attaattctt ttcttcttca aaataaatca aaatgatata
    24781 attgatgatt attttcaaag cacattcaaa tcaaactatg ttttagcaat ttgttgttag
    24841 catgtttgtg ttcattaaaa aaacgaccat catcggtatc atgtatggtc gttacaaaag
    24901 ctaacaatac caattgtcat aacaagtact gcaacctctt taaattcaat tatttcatgt
    24961 aactatagcc tatatcatat gtaattactt tgttatttat aatcgggcta ctttcatctt
    25021 catttttact tctaacatgt ttatgcgctg ctttaaagac atcagatttt aaccaatccg
    25081 taaaagcttg ctttgatttc caaactgtta aaattttcac ttcatcaaaa tcttcttgtt
    25141 ctaaagtttg tgtaacaaac atgccatcaa agccttctaa tgtttcaatc ccatgtctcg
    25201 tgtaaaatcg ttctataata tcttttgctg ttccttttgt taacgtcagc ctattttctg
    25261 ccataaattt cataattatc ctcttttctg tttaacttac cttaattatt tttgcgacaa
    25321 caacaattct tttcgtcgtt tcactatatg catcttcgca cgttgataaa gtcattattc
    25381 tatcttttac cgttacatta acatctgaat taattacaga tttacgtttt gtctcatcta
    25441 aaaattgttg ataatcttga tcattttcaa aatctgtacg tatgtaatta tctttagtag
    25501 tagttttata tgcactaaat acttgcaatt gatatttacc atatttattg tcaaattcaa
    25561 ttatcttgtg tttttcataa aacgattgct ttaaataatc ttctaacaca tcaaacatcg
    25621 tattatcacc gacatggtgc ccgtataaaa tagtattatg atttaaattc ttcaattcat
    25681 ttctaaaatc cataaaaata ctacctttac gtcgatgttc tcgctcaaaa tctaaattta
    25741 aataatcgtg atttgtctta ccttgtagta ctggataatt taatgatgtt cctgataatt
    25801 ttatccatcc aacaatgtct ttatttattt tttcaagtga ttcaaattgt ggtctcacat
    25861 gttcttgatg tttgctcatc agcatttgaa atttttgttg taatttctca taatttgcgc
    25921 gttcttgctt gtcttcaata tatgtttgaa caattttgta accaaaaatg ataataatta
    25981 caaccaataa aatttgtaca atagttaaaa atcgcttcat tctcataaaa atcctctttt
    26041 attaacgacg tttcttcagt catcactaaa ccagttgttg taccgtttta gattcgattt
    26101 cgttgacttt gacaaattaa gtaaattagc attggaccac cgacaatcat taaaatagca
    26161 ttggctggaa tttctaaagg aggctgtatc actcgtccta ataaatcagc cactaacaat
    26221 agccatgcac caataactgt agaaaacgga ataagtactc tgtaattgcc cccaactagc
    26281 tttctaacca catgtggcac aataatacct aaaaaggcta gttgtccaac aatcgcaaca
    26341 gttgcacttg ctaaaaatac tgctaataaa cctgttaacc atctgtaacg atcaatatta
    26401 aaaccgatac ttcgcgcttg tatgtcgtct aaatttagta aattcaattt aggggacaat
    26461 agtaatgtta atattaatcc caataatgct gatactgcta atatgtatac gtcgctccat
    26521 attttcattg ttaagccttg aggaattttc attaaagggt tttgagttaa aatttctaaa
    26581 acaccattta ataatacgaa taacgcaaca cctactaata tcatacttac agcattgaat
    26641 ctaaatttag aatgcaacaa tataattatt aaaaatggta ttaaacctcc aataaaactt
    26701 aataatggta agtaaaagta caattgtgga ataaacaaca tacaaagtgc tctcattata
    26761 agtgcacctg aggaaacgcc aatgatattc gcctctgcca aaggattttg tagtgctgct
    26821 tgtaataatg ctccagaaac tgctaacatt gcgccaacca tcaatgcaat taatatacgt
    26881 ggcaatcgca aatcaatgat tgaatccact gcttcattgc taccagttgt aaattttgta
    26941 aataggtcat taaatgacaa tttaattgta ccggttacaa acgaaatata agcagttgcg
    27001 attaaaatga ctaacaaaca taaaaatagt agtttctttt tattttttat catcatatca
    27061 actcactatt ttttatcctt ataaaataaa tcatataatt gagtcattgc cttatcagca
    27121 tcaacatttg ctgtaatacc gaatggcact tcctctaagt cataaacatg attatttttc
    27181 acagctttaa aatgtttcca aatatcattt tgtttaaatt ctttttgaaa cattttctta
    27241 acttcttcag gcattccgtg tggtaatcgt aaaataatat ctggattgat attcaacaaa
    27301 ttttcagtat tagacgaaat atattgacga tctttcactt taataacatt ttctccacct
    27361 gctattttaa ctaaatcacc aatatatgat ttatcagttg ctactaaata gctacccggt
    27421 acacccatta atattaatac tttgggatgt ttcttttgtt tagctgcttt attttcaatt
    27481 ttttgcttta cagaatttaa atggtcattt aattcttttg cttgtgcttt acgattaaat
    27541 tgatcaccta attgtgtaat cgacttttgc atccctttta aactatcaaa atcataaaag
    27601 tagcctttca tatttaattg tttgtaaaat ggttgcattt catctttaat cgttgacaca
    27661 ctcaaaacat gtgttggttt taacttttta acagcttcaa catccggctc cattggttga
    27721 ccaatttccg gtacatcttt ataacgatta ggcaatgtct tatatgacgt gggtttgccg
    27781 acaattggta aatccaattt gtccaatgtc attgtcaatg caacagttgt tggtacgatt
    27841 ctgaattcgc cggatttagt tgattcttga gaactggaag attgacagct ggttaagata
    27901 acgacgctta tcactaaaat ggttaaatac tttatgattc tcaaacttgg ttacctctct
    27961 ttctgctgta taaatgtgcc gaaataagta aaatagttgc taacacaatg ccagctgcaa
    28021 ataacatata ttgcttttta tgatttggtt catcaagttc tttaatatca ctagataatt
    28081 gactactatt cttgctatca tcatctctat taaaagctgg taacgctttt tctttttcaa
    28141 ctactttttt cttttccttt tttgattttt catctttatg tttcggttgc tctttgtgat
    28201 tattttcaac cttttttatt gttggagggc tattttcaac ttttaatgta cctgatgctt
    28261 taggtcgctc attttcaatc tttttattac tgtcttgttg cactttatct ttttgtacag
    28321 gaatatcatt taatcctttt tcagtaaaaa ggctagtacg tctttcctta gtatctgatt
    28381 tttgactatc ttttttgtca tcaatatctt ttttaagctg atcttgatta ttttcttgat
    28441 tttgatgttt tgaaggcact tcttttgcat catcatgttg tacttttttc tctttaaaat
    28501 gaatagaata caccatttct ttgttatcta gatttttctt ttcagccaac gttttaacat
    28561 gcatattgat tttatccata tgctgttcaa ttaaaattct tactattgtg tcatcctttt
    28621 catcttttat aacaaaagga aattcttcat ttttgtcatt ttgaaaaaca agttgttctt
    28681 tatctatttg accttttagc tttacttgca agtaaatttt accagtcata tctgaaatca
    28741 attgcgcctt ttcgtcaact agttgttgtt cctcagttac atttttattt tcttgattaa
    28801 aaattagtaa ctctaacgct ttattctctt ttatcttttc atcaaaaatt gatgtagtta
    28861 attctgtttt atcttttgct tcaggttgat taaatttggc tgatggatat tttatctcat
    28921 cagccaacac gctaccaatc attgttgtat atgtaagtat acctaagcta ataatagcta
    28981 taatagattt tgtagcaatt tgtttaacat ttctcattat tccacattgc ctttagattt
    29041 ttttctaaca aacaatgtga ttgcgat
//
LOCUS       DANRDI010000017        28430 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.017, whole genome shotgun sequence.
ACCESSION   DANRDI010000017 DANRDI010000000
VERSION     DANRDI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.017"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            1..89
                     /locus_tag="PZ377_000575"
     tRNA            1..89
                     /locus_tag="PZ377_000575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Ser,seq:gct)
     gene            complement(269..1780)
                     /locus_tag="PZ377_000576"
     CDS             complement(269..1780)
                     /locus_tag="PZ377_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDM6357466.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
     gene            complement(1919..3277)
                     /locus_tag="PZ377_000577"
     CDS             complement(1919..3277)
                     /locus_tag="PZ377_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDM6357467.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            complement(3294..5618)
                     /locus_tag="PZ377_000578"
     CDS             complement(3294..5618)
                     /locus_tag="PZ377_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDM6357468.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            complement(5837..6640)
                     /locus_tag="PZ377_000579"
     CDS             complement(5837..6640)
                     /locus_tag="PZ377_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDM6357469.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            complement(6942..8504)
                     /locus_tag="PZ377_000580"
     CDS             complement(6942..8504)
                     /locus_tag="PZ377_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDM6357470.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            complement(8504..8764)
                     /locus_tag="PZ377_000581"
     CDS             complement(8504..8764)
                     /locus_tag="PZ377_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDM6357471.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            complement(8748..10229)
                     /locus_tag="PZ377_000582"
     CDS             complement(8748..10229)
                     /locus_tag="PZ377_000582"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDM6357472.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            10662..11837
                     /locus_tag="PZ377_000583"
     CDS             10662..11837
                     /locus_tag="PZ377_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDM6357473.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            11815..13005
                     /locus_tag="PZ377_000584"
     CDS             11815..13005
                     /locus_tag="PZ377_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6357474.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(13118..13627)
                     /locus_tag="PZ377_000585"
     CDS             complement(13118..13627)
                     /locus_tag="PZ377_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDM6357475.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(13821..14579)
                     /locus_tag="PZ377_000586"
     CDS             complement(13821..14579)
                     /locus_tag="PZ377_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDM6357476.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
     gene            complement(14721..16289)
                     /locus_tag="PZ377_000587"
     CDS             complement(14721..16289)
                     /locus_tag="PZ377_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDM6357477.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            16631..17716
                     /locus_tag="PZ377_000588"
     CDS             16631..17716
                     /locus_tag="PZ377_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDM6357478.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(17911..18681)
                     /gene="fabI"
                     /locus_tag="PZ377_000589"
     CDS             complement(17911..18681)
                     /gene="fabI"
                     /locus_tag="PZ377_000589"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDM6357479.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(18959..20803)
                     /locus_tag="PZ377_000590"
     CDS             complement(18959..20803)
                     /locus_tag="PZ377_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDM6357480.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(20813..22198)
                     /gene="mgtE"
                     /locus_tag="PZ377_000591"
     CDS             complement(20813..22198)
                     /gene="mgtE"
                     /locus_tag="PZ377_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDM6357481.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(22219..23073)
                     /locus_tag="PZ377_000592"
     CDS             complement(22219..23073)
                     /locus_tag="PZ377_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDM6357482.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(23070..23879)
                     /locus_tag="PZ377_000593"
     CDS             complement(23070..23879)
                     /locus_tag="PZ377_000593"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDM6357483.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(23896..24531)
                     /locus_tag="PZ377_000594"
     CDS             complement(23896..24531)
                     /locus_tag="PZ377_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDM6357484.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(24548..24895)
                     /locus_tag="PZ377_000595"
     CDS             complement(24548..24895)
                     /locus_tag="PZ377_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357485.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            25081..25674
                     /locus_tag="PZ377_000596"
     CDS             25081..25674
                     /locus_tag="PZ377_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDM6357486.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            25778..26143
                     /locus_tag="PZ377_000597"
     CDS             25778..26143
                     /locus_tag="PZ377_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDM6357487.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            26166..26972
                     /gene="yjbH"
                     /locus_tag="PZ377_000598"
     CDS             26166..26972
                     /gene="yjbH"
                     /locus_tag="PZ377_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDM6357488.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(27432..>28430)
                     /locus_tag="PZ377_000599"
     CDS             complement(27432..>28430)
                     /locus_tag="PZ377_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F family protein"
                     /protein_id="HDM6357489.1"
                     /translation="VYDNLVKTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKD
                     IKFEMPYEEAKEWMLKALEPMGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLT
                     NPFILLNWSNTISDLYTLVHEFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALL
                     SDYMDKHLDDEKRLLLLNQELERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNE
                     EYAKLNKLYFGDSVETDEDISKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGK
                     PAVDRYINEFLKKGSSNYPIEILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
BASE COUNT         9401 a         5167 c         3956 g         9906 t
ORIGIN      
        1 ggaaacgtac tcaagttggc tgaagaggcg cccctgctaa gggtgtaggt cgcgaaagcg
       61 gcgcgagggt tcgaatccct ccgtttccgt tacttgctaa aatggtatat accattttag
      121 cttttttgtt tacttatata taatgaatga gaatttcact gttcttttat atcaatttta
      181 aaattctaaa aacctttcct agataatctt ctctaagaaa ggtttttata cttgttgaac
      241 ttataattaa tttattacat agcaatattt acctgttttt aactataaaa ttatcactac
      301 atgaaatacg ataattcgga tctcttaact tctctgcaat taatgtactc attgtttcat
      361 cgtatgattc atgtataata gcatttttta aataattctt gtagttacga taacaatagt
      421 cagtcattgt aactctataa ctcttagtca aatcaatttg atctaacatt actcgttgcc
      481 caggttcttg attcatatca atctcataat tgaatccttg ccacattgta cataacgttt
      541 catcaataat tgttaagctt aattgctcgt taacaaaatc taaatgtgaa taactatact
      601 ccaatatatc tttgatattt tgaccactaa ttgtcatatc cattggctta tctggatatg
      661 ggtatgcatg atacaaatct tcatttcgaa tctgtccact caaccccttc tctccgttca
      721 taggcacgtg gacacatgtt atatcattat caaatgcaag gtgaattgca tcatgtaata
      781 attgcgaaaa tggatgtgtt tgacagacta aatcttgtaa cccatttact gataacatca
      841 aacctttatc acttatgatt tcctgtgacc aataagccac tgcttttcta tcatagaatg
      901 ttaaatctaa taattcttga tcctcttcat actcatttaa gtcaatcact ttagaatcaa
      961 tgctttcaac atcataagtt gttgttctct ttttaaaatt aatcgaaaga tgtacaagct
     1021 ctttggcatc ttgaccagcc tgaacataat acgtttcatg atcttgacct actattgttt
     1081 gatgctgatg agctgtaatc attaaatcta taacaccgag ttcttccatt aatttttcag
     1141 cttcattcga acttgccttt ttatttttcg tactattact aattttattc aatccaccat
     1201 gataaattac aatcaaaaaa tctggctctt caacttcatg gatatatcta atccaacgtt
     1261 ttgatgccac taatgtcttt tcaatagata catcttgctc catttcagaa tactcatttt
     1321 ccattaaacc atctgctgtg acgcctacga tagcaatttt taagtcacca aaatgtttaa
     1381 tacaatatgg agttgaaaaa taaggctcct tagtaacatt gtattcaata tttgctgata
     1441 accatggaaa acgtgccaaa gcaattgaac gagttaaaaa tgataaacca aacttgaatt
     1501 cacttggact cacaccgcta gcatcataat gcattctgtt cattaacttt atcattggat
     1561 gtcgtttata aggtgcaaca atagcataat aataggccgc taacgagcca gctaaacttc
     1621 cgccactatc taataaaatg acgcgatgat tttgtgctct tacttggtta acataagtac
     1681 cagctctata aatatttgaa ccataatcac catttaagaa atgactatgc atatctgacg
     1741 tagttaaaat ctctacatta atgttttcat ttttttccat catgttacac ctctcattgt
     1801 tactagttta ccattttcga aaccaatcgt cttataaaat aagttaaaaa tacaaactaa
     1861 aaaagcaatc taagttccag ttaaaatcta cattaactaa acttagattg ctattatatt
     1921 atcctatttg aatacgttct tttggataat ggaatttatc tggttctcga cgtcctgcta
     1981 tcataatgat aaatgatatt aagccaacac gtcctataaa catgagtatc attagtacga
     2041 ccttagaaat atcactaata tcacttgtga caccaagcga tagtccacaa gttccaaatg
     2101 cagacatgac ttcaaaaaat acttgtaaaa atgtaagctt accattttca gtagctgata
     2161 taattagcat tcctaaaaat gttaaaattg tcgccattgt aaatactgca aatgaacgtt
     2221 gaatatccat aatgtgtact tctctattgt aaactttaat ggatgtttta tcggcattat
     2281 tactaaagtt aattaaaaac aaaattaaaa tagcgaaagt tgttgtacga ataccgccac
     2341 caaccgaact tggcgaagat cctataaaca ttaaaatacc catgataata ttagtggggt
     2401 cacttagtgt tgtcacatca attgtttgaa gacccgcact tcttgtagtc gctgattgaa
     2461 acagcgaata gaataacgat tgatgccaac ttaaaccttt gaacgcatgg ttatgttcaa
     2521 ataatagaat ggctaatacc ccaacaataa ataggaataa atatgttgat gtcgtaattt
     2581 tagtaaataa tgaaaatcta aaattagtaa ccctattttg aatataagct ttaacttcta
     2641 ataatactgg gaagccgatt gatcctaaaa ttattaaaaa tataactatc gcttgtacaa
     2701 aataatcatg tgcataagga attaatgact tacctgtaat atctaatcca ccattggtag
     2761 tggcagaaat agaaacaaaa acaccttgca tgattgcata ttttaaatct ggattatctc
     2821 gataaaagta aaatgctaat aacatggctc ctacgagttc gattacaaat attgatttta
     2881 caatatcaat aatcaatttg acggtaccac tcattgtgtt tttgttatta tctaacataa
     2941 ttaactgacg ttctctaatt ccaatatgtt tacctagtac cacccataac atcgtaccaa
     3001 ttgccatgac gccaattcca ccaatattta atatcacgag gataattaat tgtccaaatg
     3061 tagaataggt atcgacaata cttatcggag acaatccagt aacactaatt ccggatacgg
     3121 caacaaataa tgtgtcaatt ggatttactt ctacacctgg tttatgaaca tacggtaagt
     3181 ttaataataa aaatgcaatg acaattgcga cgatatagta caatacaata ccttgttgag
     3241 ggcttgatct ttttaaaaac tggctaaaaa ttgacacgtt gttcacctca acattatttt
     3301 agtttaatat taatttcttt ctctttacca tctctataaa tcttcgctgt aattgatttc
     3361 aagtcatctt tatgactaaa tataatctgc ctaaagcgta aatcatcttc taaaagtttg
     3421 ccatctaatt cagtaattac atcacctttt ttcagaccag attgatctgc taaaccattg
     3481 ttgtcaactt gatctacaac aacaccgttc ttaacttttc ctggcaattt aactgcttgt
     3541 ctttcaaaac tatttagact ggcaatattc ttcattttaa cacctacatc gggatagtca
     3601 attttacctt ttgtttctaa atcttttaca atcttttgta cttcattaac aggtattgca
     3661 aatgacatat tttcaacgtt tggcatacta attttagctg caactacacc tattaatttt
     3721 ccttctctat tgacgacagc accacccgag ttacctggat ttactgatgc atcaatttgg
     3781 aaagctttca tcaacatatc atatttatta tctttatcga aatcaatagg aacatttctg
     3841 ttcagacctg aaataatacc ttctgtcaca gtgcctttaa agtctacacc aagtggatta
     3901 cctacgacta atattggctc tcctaacact aaattatttg aatctccaat agctatctct
     3961 ttcactgaac tgtctgaaga agttgcttta acaacagcta aatctgacca tttatcttta
     4021 ccaagcactt tcccaacaac acttttatta ttcgagaaag ttattttttg attttcttta
     4081 tcaccgacaa cgtgtgcatt cgtaacaata tataacgtat ctccagattt tttatataca
     4141 acacctgatc ccacttcgtc ttgagatgct ttatctttag gtaatgatga atcttttgat
     4201 gtttcatttt caactgtaac gaccgattta actgtgttat tggcattttt catcgttgtc
     4261 gtatattttt gagcatctgc atcattatta tcattcatgc gatcattttt gttcacatta
     4321 ttaaaaatgg cattcaaaat aacaattaga ataataatac ccattagaat aactaattta
     4381 ggccaataca tcatccaaaa ctttttcaaa atgtcactat aattattatt cgtagcgcgc
     4441 cctttgttca attttgatgt agcctttttt tgattattat ttgccgatgt ctttgtattt
     4501 gttttttgac cgtttaattt atcatggtca ttaatagaaa tatttctttt ttcagtgcta
     4561 cgatgagatg cagttgactg actttgatat cccaccgttt cagacgagtc tgcattttgt
     4621 ttatgtttat cttcaacatg aattttttca tcaagttttc gatcgtcatc tgtttttgat
     4681 aatgatggtg tattgatatc attatcagga ttatcgtcaa caatatgatt ttctctcatg
     4741 acgttatctt tatcatcact tatcgatggt atatgatgac ttaaatcatt ttcatgatta
     4801 gacttgtcat tttgttcgct atcaccatat gtataggttt ttgtttgctc attttgactt
     4861 tgctgtcttt tcaatttctg ttcttccggt aaagttaatg gcttctgatt atttacttca
     4921 tcatcagatt ccgacaattt tggtactgta tttgatttgt ctgatattac ttcagatttc
     4981 agattactac ttttatttcc atctgaactg tcttttttta ttttctctgt ctgcatcgag
     5041 ctgtctgaat cagttcttga agactcagaa tcaattctag tctctttatc atgttctgaa
     5101 ggtttagaaa gtacatgttg ctcttcatga ccaactttgt ttttatctga acttagcttt
     5161 ctcatttcag ttgattctac tttatcggtt gatttagttg ataaagtcgc ttcagaattt
     5221 tgctcataat attttgattt cactgtatct tggtccactg aatctaatga attttgtttt
     5281 ttagatacat cgtctgttaa ataatgatct ctgttatatg ctgacttact atcctgatta
     5341 cttttacttt cttcagcttt attctcatta acatcacggt ttctctgttc taaatgcgat
     5401 gataaactat ttttaaaacg ttcgtgctta tcaattgaat ctttatgtat ttgcttatct
     5461 gctttttttg atgttgtatt atctatattt tgtttatctt gatgttgatt taaattttct
     5521 tctctgtctt cgttgtggaa gaattcacga cgcttacgtc ggtactgact tttaggaatt
     5581 acatgttttt taccaatatc cactaatgtc cactccattc aataaaatca cttatcatta
     5641 tgacacatta acacctaatt tgctttaaaa tctatataat ttctattgaa aaaatgctaa
     5701 atataaaagg cacattttct taatactata tcgcatcttt tgattcacat tcttcaaaat
     5761 ccatttttca taaatcactc aattaaatga aaaaggacaa ccttaaacta aattaagatt
     5821 gtccgcttca catcaattat ttatgcgatt ttttattttt aacaactgaa cctaaccaac
     5881 cgataattac taatccaatt agtactgtcc agaaaataga ttgccataat acgccatgtg
     5941 ggaagtgctc aggcaataca gcgatgtctg ggtgcgctaa taccatgaca actaatttaa
     6001 cacctaccca accaacgatc gcgaaggctg caccttcaag tcctggatat ttgtttaata
     6061 gctctacaaa ccatgttgct gcataacgca ttagaataac accaatcatt ccacctagga
     6121 acatgactac gaactgacct aagtccatac caccaaagtg aataccaact ttaggaagtg
     6181 ttacagcaat agctaaagca gcaagcatag aatcaatggc aaatgcgata tctgcaaatt
     6241 ctattttcaa cacagttccc cagaatgatt tgttacttgc tttaatctct tcaccagatt
     6301 catcataatg atgatcatct ccagcttcag gactttcaat ttctgggtgt ttaaagaact
     6361 gccacagatt tttgattgac atataaatta agtaaaccgc tcctgcagct tgaataaacc
     6421 aaaagttcgc gataatacta attaagaata atgctaaaaa tctaaataca aatgcaccta
     6481 acaaaccgta aaacaaagct tttttacgtt gttcgggtgg taagtgctta accattacag
     6541 ccataacaat cgcgttatct gctgctaata agccttctaa aaatactaaa acgacaagta
     6601 cccataaata aggtaagatc aaacttggat ccattaacat gtacaactcc tttaaataaa
     6661 taagttaagt acaaacaaaa agacctatgc taaataaata gcaaaggtct cactaagcaa
     6721 tttaaattgc ccactttacc ggaagatatc ttcgtaatga cgataaagtg tcgaatcatt
     6781 tatacggtca tgattcttaa gttactcccc tctgacaaaa tgccataggt tattcatttg
     6841 atacatcata gtataccttt ttcttttaaa atatacaatt tatttttctt caacgaaaca
     6901 attattttta atgtaacaaa ttcgattata caattgagct gttaaagtaa actatacaat
     6961 ttaatttcag ggaatttctc ttcaaaccat cttgttgcaa attcattttc aaataagaat
     7021 actaaatcgt catatctatc tttcactaaa atcgatcttg atgtgttcat cttatctgta
     7081 atttggtctt cattttcaat ccaacgtgca attttacggc ctactggctc cataacaaca
     7141 tcaacattat attcgttttt cattctatgt tcgaaaactt caaattgtaa ctgaccaaca
     7201 gcacctaaaa taatttggtt tgtgtgtaat gttttatagt attgaatcgc accttcttgt
     7261 actaattgtt caataccttt atggaaatgc ttctgtttca tgacgttttt agcagaaact
     7321 ttcataaaaa tttctggcgt aaattgtggt aaatcttgga aactgtaggt ttgttttcca
     7381 ccaactaaag tatctccaat ttgataatta ccagtatcat atagtccaat gatatcgcct
     7441 gctacagcat gattcacagt ttctttatcg tctgccataa atgacgttga acgtgtgatc
     7501 ttttgctttt tattagtacg ttgcaaagta acatccatac cacgttcaaa tgcaccacta
     7561 acgacacgca taaaggcaat tctatcacgg tgtttagggt ccatgttggc ttgaatttta
     7621 aagataaatc ctgaaaatga atcatcaaac gggcttactt caacgtcttc ttttgtttgt
     7681 ctcgcatttg gcattggcgc aaaatcaaca tatgcattta agaaattttg tacaccaaag
     7741 ttagctaaag ctgaaccgaa aaatacaggt gttaagtctc cactcaacag cgcgtcatta
     7801 tcaaaggctt cacccgcttc ttcaaccaac attaattctt caatcgcttg ttcaaaatca
     7861 ctatcatttg taattgcatg atcttcttcc aactcaaaat catcattcaa atgtaatata
     7921 ttttcttcat ctctaaatgg ttcaattgtt ttagactttc tatcaatgat gccaaagaaa
     7981 ctttgtccca taccaattgg ccaattcata gggtatgttt caatatttaa tgtctcttcg
     8041 atttcatcta ataattcaaa tggttcttta cctactcggt ctaatttatt aatgaatgta
     8101 aagattggaa taccacgcat tttacaaact ttaaataact tcaatgtttg tggttcaata
     8161 ccttttgcac agtctatgac catgacagca ctgtcaactg ccattaatgt tctatacgta
     8221 tcttctgaaa agtcttcatg tcctggtgta tctaagatat tgattttata atcatcgtaa
     8281 tcaaattgca ttactgaact agttacagaa ataccacgct cttgttcaac tttcatccag
     8341 tcacttgtcg caaatttacc agtcttcttc cctttaactg tacccgcttc acgaatagca
     8401 ccactgaagt acaatagttt ttcagttaac gttgttttcc ctgcatcggg atgtgaaata
     8461 atcgcaaaag tctttctaga ctcaacttct tgctttaagt tcattagtct ttctcctttt
     8521 tatatgtttc tatccaatct gttagttgtg atgcaatcga ttctgcctct tcctcttctt
     8581 taaaaacaaa caattgcatg agtattgctt caaaacaagt ttcttcatct aatgtcattg
     8641 ccatctgata tgaccaaaac caatctggta tagcaatgat tatctggtta tttaatgctt
     8701 cgaataaata aacatcaata gttttacctt catcaataaa tcttttatta atcaacaggg
     8761 ccaccaccga actttttgta agctgcttca aggccaatta aatcatctcg atgtggcacc
     8821 ttaatatgcc ctggcatgat ttgatatggt tctcttcctt ttgatgctaa aacgacagta
     8881 tccccaggct cagctatgtc aattgcatgt tttatccctt ctgcacgatc atcaaattca
     8941 atatagtttt gatgtgttgc acctttggct aattctgccg ttaacatttt cgggtcatca
     9001 tttgccggat tatccggtgt gaaaatgaca taatctgcac gacaggcaac tcgccccatt
     9061 tcaggcgttt tagttaaatc acgttcgcct gccataccaa ctaaaaatat caacttttgc
     9121 tttacaaaag gctgtactgc atcgattaat ttgttcatac catcagctgt atgtgcataa
     9181 tcgataatta aatcaatagg taacgaagga tctaaaactt ctaatcgccc ttcaacaggt
     9241 tctaaatttt caacagcttt aataatcgtt tctaaagatg tacctttact ccacaccgca
     9301 atcattgccg ccataatatt agaaatatta aacttaccaa cataaggcga ttttactggg
     9361 taagttccaa aaggcgttac aaaatcaaag ctgacacctt gtaaagattc ttgaatattt
     9421 ttagccataa attgggcttc ctcatcaatt ccataactaa atacttcata aggcgtcact
     9481 gttcttaaat actcagaaaa tgaatcgtca ttgtttaaca cgacatactt ttctttcgac
     9541 aaatcttcac ctaattgact aaacaataaa gacttcgcgt gtccgtatgc ttccattgtg
     9601 ccatgaaaat ctaaatggtc ttgtgttaaa tttgaaaata ttgcaacgtc aaattcaacg
     9661 cctcgcagtc gtcctaatac taagccatgg cttgatactt ctaatgtcat agattcagcg
     9721 cctgcatcaa ctgcttcttt aattttctta gttaaagaaa ctgtttctgg tgtcgtattt
     9781 gcaccttttg tctttgtttc attaatttgg aaaccattag ttcctaaata tgcactattt
     9841 ttttgtaact ttctttgaat taaatgaatc atcgtcgcaa tagaagtttt accatttgta
     9901 cccgttacac caaatgtcac taactgatga ctcggataat catataatgt gtgtgctaga
     9961 atactagcta ctcttaatgt gtccggcaca accacttgtg ttacgttagc tggtaatgat
    10021 tgttctttat tgaccactac caacttacac ccttgatcag ctacattttg acagaactta
    10081 tgactgtcta cagtatatcc aactgaagcg acaaaaatgc taccttctct cgctgtacgt
    10141 gaatcagtag tgatatcatc tatttgttgt tctaaagaac ccaatacacg ctttactttt
    10201 actttcttaa acaacgtact tgcatccaaa ctgaaccgct cctttttcta caatatgttt
    10261 attatacacg ttttgttgat gttaataaat ttataatttc actgttatat cctactaatc
    10321 agcattttct aatttctact attaaaaata aaacatacgg tattatgaat tagttttaca
    10381 attatggctc atcttaaatt attaaaacca tcttgtaaca tggtataata tcataattgt
    10441 ttccaaaaat atgctataat tgtatactgg ataattttaa atatacaaat aaaacataga
    10501 acaataaatt gctttataaa ataacgtttt attttgtatc aattcatcta tagattaaaa
    10561 ttgctataaa tgtaacgact ttgcgtcttt atttctttta taatgccgta aaataagtta
    10621 cacttaaata aaattttcaa actaacggag ggtggctatt aatggttact caaaataaaa
    10681 agatattgat tattactggc tcattcggta acggtcatat gcaagttaca cagagtatcg
    10741 ttaatcaact taatgatatg aatctagacc atttaagcgt cattgagcac gatttattta
    10801 tggaagctca tccaattttg acttctattt gtaaaaaatg gtatatcaat agctttaaat
    10861 attttagaaa tatgtacaaa gggttttatt acagccgccc agataaacta gacaaatgtt
    10921 tttacaaata ctatggactt aataagttaa ttaatttatt gataaaagaa aagccagatt
    10981 taatattatt aacgtttcct acaccagtta tgtcggtact aactgagcaa tttaacatta
    11041 atattccagt tgctacagtg atgacagact atcgcttaca taaaaactgg attacgccgt
    11101 attcaacaag atattatgtg gcaacaaaag aaacgaaaca agacttcata gacgtaggta
    11161 ttgatccttc aacagttaaa gtgacaggta ttcctattga taacaaattt gaaacgccta
    11221 ttaatcaaaa gcagtggtta atagacaaca acttagatcc agataagcaa actattttaa
    11281 tgtcagctgg tgcatttggt gtatctaaag gttttgacac gatgattact gatatattag
    11341 cgaaaagtgc aaatgcacaa gtagttatga tttgtggtaa gagcaaagag ctaaagcgtt
    11401 ctttaacagc taagtttaaa tctaacgaga atgtattgat tctaggttat accaaacaca
    11461 tgaatgaatg gatggcatca agtcaactta tgattacgaa acctggtggt atcacaataa
    11521 ctgaaggttt cgcccgttgt attccaatga ttttcctaaa tcctgcacct ggtcaagagc
    11581 ttgaaaatgc cctttacttt gaagaaaaag gttttggtaa aatcgctgat actccagaag
    11641 aagcaataaa aattgtagca agtttaacta acggtaatga gcaattaact aacatgatta
    11701 gtacaatgga acaagataaa attaagtatg ctacacagac aatatgtcga gacttacttg
    11761 atttaatcgg tcattcatca caaccacaag agatttatgg aaaggttcct ttatatgcaa
    11821 gattcttcgt taaataatta cgctaatcat aaaaatttca ttttaatgct tattatctta
    11881 tttttaatgg aatttgcgag aggcatgtat atcctaagct atattaattt cttgccaaca
    11941 gttacatcca ttgctgtagc aataacgtct ctagcatttt caatacattt tattgctgat
    12001 gcatctacaa actttgttat aggattttta ctgaaaaaat tcggcacaaa aatcgtttta
    12061 acgactggat ttattttagc atttacaagt ttatttttag ttatttggtt tccagcatca
    12121 ccttttgtaa tcatatttag tgctatgatg ctcggtattg cagttagtcc tatttgggtc
    12181 attatgttat ccagtgttga agaagataaa cgtggcaaac aaatgggcta tgtttatttt
    12241 tcatggttgt taggcctatt ggtaggtatg gtttttatga atctacttat caaagtgcac
    12301 ccaacacgct ttgccttcat gatgtcttta gtcgttttaa ttgcatggat attatattat
    12361 tttgtcgatg taaagttaac aaattataat actcgtccag ttaaagctca attgagacaa
    12421 attgttgatg tgacaaagcg ccatttattg ttgttccctg gtattctttt acaaggtgcc
    12481 gcaattgctg ccctagttcc tatattacca acatatgcta ctaaggttat taacgtaagc
    12541 acaattgaat atactgttgc aatcattatt ggtggtatcg gctgtgcagt ttcgatgcta
    12601 tttttatcga aactaataga taaccgcagc agaaacttta tgtatggtgt tattctaagc
    12661 ggatttattt tatacatgat attaattttt actctatcta tgattgttaa tattcacatc
    12721 ttgtggatta tcgctttagc tatcggtcta atgtatggca tcttattacc agcatggaat
    12781 acctttatgg cacgatttat caaatcagat gaacaagaag aaacttgggg tgtttttaat
    12841 agtatccaag gattcggctc aatgataggg ccattatttg gcggattaat tacacaattt
    12901 accaacaatt taaacaatac attttatttc tcggcattaa tctttttagt cttagctgta
    12961 ttttatggaa gctactttat agtaaataga gaaaaagcta agtaaggtta aaacgtattt
    13021 taaaataaag aaaatggtca tttttattac attcaataaa aatgaccatt ttcttattaa
    13081 ttcaaacata ctacgattaa caatactatt atgttactta agctaattta aacgtttcaa
    13141 taactttcca tttatcttcg tcatcgtcaa aacgtaacaa agttaattca tcgataattt
    13201 ctttatggtc taccccagct aatgctactt gaccaaaaat atcttcgaat tcttggctag
    13261 atagtccttg tgctattgta aagtgtggca caaaaacatg ttcagcttct ccatagaaat
    13321 cttctccatt aaagcgatta aacaattctt ctaagtcgtc cgtcttcgca actttaaagt
    13381 aaatcacatt gttcgttggt ttgaagctag aagcttttgt agcatgaact tctactgctg
    13441 gtataccatt aatacgagct ctaacctgtt caatgacaga atctaaatca ccatctttaa
    13501 tttcaaatgg cgctttaatt gtcacatgtg gtttaattcg tgaatactgt ttatcatatc
    13561 ttttacggta agaatccacc gcttcttgaa atgactttga tggaattaat gctaatccta
    13621 aaatcatctg aattcctcct ttgtttaacc tattgctatt atatcaaatt tctttgaaaa
    13681 ccgttacatt aaaaataatt cattatacat cttttgcaat ttcgtcaaat ttaaattaaa
    13741 ttaaacagta aaatctttta taaattatat tatagaccag actatatgaa gacaattcat
    13801 actaaatcta ctaatgtaaa ttattcataa agttgatcat ctgtgttttt acttaaaaaa
    13861 tacaataata tatctgacag caatggtttc caatctttcc attggtgacc accatcaaat
    13921 tcatcgtaat aataagttat attatatttc ttaatttgtt cagctaattc tctattcggt
    13981 gttaagaaat cggcacgctt accatttgtt ggtaaagtaa aatctttttc atctagacca
    14041 atgacatgcc aaattgtcaa ttgttcttta tttgcacatt gatttagctt atctaatact
    14101 ttttcatctg aatgtggact taacattgct acacgactaa aaatcgttgg ataggtcaac
    14161 gccgttaata aggcaatact acccgctaaa ctatccccta ctaataacct tgcattacct
    14221 actttcagtg tagaaaacgt cgagtcaata aatggcaata tttctttacc gactgattga
    14281 atagttaaat gagaacgact tccttgtgga tgaaattcct ctctacgctt atcaacgtct
    14341 tcataatgga atccaacaat gatcgcatca tcaatacgcg cttctttgat taacgattca
    14401 tatgtacgtt gtattctccc gaaacgtaaa aaatctaatc cgtcaaagca aagaatgaca
    14461 ttatatttaa caagttgatt ataagattct ggtaaataaa tacttaacgt gacatctcta
    14521 tttaaaatat tactatataa aacatgttta ttaatcttac cagttttaaa ttctgacata
    14581 aaaaagtgcc cctagtagtt gtattgttat tgtaccaaga gacactatat ttttaaaatt
    14641 caaatttctg agcacctaaa attctcaata cttttctttt ttatcaaatg atcactacat
    14701 atactataaa caaaagtaga ttactttttg gcacgtgctt gttttaaacg ttctggatac
    14761 gtcttcaacc aaaagacagc attaggtaat ttattcggat caggttgata aactgcatta
    14821 taaccgttgc ctaaacgttt cttttgaatt tcataatctt ttaaagcatt tacggcaggc
    14881 ttatgtaaaa tccaaatcgc aatgatattt aaccaagcca ttagacctac acctaaatca
    14941 ccgaatgccc atgctacatc agctgtttta actgcaccgt aaaatgtagc gaacaaaata
    15001 atcacacgag caatattaat aaatatcgat gaaacttgat tattttgatt acgcgttaaa
    15061 taagcaacat ttgtttctgt aatgtagtag tacgacaaaa ttgtagtaaa tgcaaagaag
    15121 aataaagcaa atgcaataaa gtacgaacct acgccagaga aagtaggatc aaattgataa
    15181 ccactgccat ggaacgcttt atcaatgccg gcttgtgcat acatcgcagt acctgaataa
    15241 tctttacctg ttgcattttc aacataaata ccgccatctt taattaaatg cggtgtgcca
    15301 ttcgcattaa ccgtaccatc agtcacatta tatgtaccag aaataagtat aatcagagca
    15361 gttgcagtac atacaaataa tgtatcaata taaactgaaa atgcttgtac tagaccttgt
    15421 ttacttggat gtgatacttc tgctgccgct gctgcgtgtg gacctgtacc ttgaccagcc
    15481 tcatttgaat ataatccacg tttaacacca atctctatca ttgcgccaac gataccacca
    15541 aaagcagatt gtaatccaaa tgctgatttg aaaattaatg caaataacgc tggcacttct
    15601 tgtatattga tacaaatgat aatgacagcc atcagtatgt aaataattgc cataaatggt
    15661 acaacggctg ttgcaacatt ggcaatacta cgtacaccac caaaaataat taatcccaaa
    15721 ataactacaa caataccacc cattaaccat tgtggaacat gaatcgcatt atgcatagaa
    15781 cttgctatag cgttagattg cacaccagga agcaatagac ctactgagat aatcgtaaca
    15841 atagcaaaga taattccgta aattttacca aatttaccac caataccata ttcaatataa
    15901 tacgctggtc caccacggta ttcattattt tcaactctct tgaatatttg accaagtgta
    15961 gattcaataa aagcactact tgcacctaaa aacgcagtaa tccacatcca aaatactgca
    16021 ccaggtcctc ctataaatat tgcagtagat acaccgacaa tattacctgt accaaccctg
    16081 cctgctaaag acatggctat cgcttgaaaa cttgaaatac cattaggaga cttctctcct
    16141 tgaaacatta atcgaatcat ttctttaaaa tgtctaactt gaaaaaatcg catacgcaat
    16201 gtaaatagca caccagtaat tagcaaacca taaactaatg gcttactcca aacaacctca
    16261 tttaaaaagg ttactaattt ttcaatcata agattccccc tattgtttaa acataaaaat
    16321 attatacgat taagcacgag acacttcaat atatttttca gaatattctt ataaatatta
    16381 atatgatcat ttcactattt aaacacgttt aatattaaaa taagttattc atataaactg
    16441 gtgctgtttg atccaattgc agacttacga gtcattgaaa agtctcacaa actattgaaa
    16501 gtaaatatct taaaatagaa agtgaatggt taatttaagt atatttaaaa aatattaacc
    16561 tttttaagca ctgctattta ggatatacta aataataact aagtttagaa aaataggagg
    16621 aacatcgttt atgttaaaca aggtttggtt ccgaactggc atcgctctga ttatgctgtt
    16681 cattctcatc aaactattta tggaagtgca cgaagtattt actccaatag ctacaatcat
    16741 cggttctgta tttcttccat ttttaattag tggtttttta ttttatatct gtctaccttt
    16801 tcaaaactta cttgaaaaag tcggttttcc aagatgggct agtataacaa caatcatgtt
    16861 agctttgttt gctattatcg gcttaattgt tgcgtttgtt gcaccaatca tcatttcaaa
    16921 tatcaacaat ttaattagtc agacacctgc cctacaaaaa gaagcagagc aaattattaa
    16981 attcgcacta gctcaaatgg ataaattacc tgaagatgta acaagtagaa ttacaaatat
    17041 ggttaaatca atgggtgatg gcgttactaa tattttatct aattcattac agtacattac
    17101 atcattaatt tcaaccatct tcttattgat tatggttcca ttcttcttaa tttatatgtt
    17161 aaaagaccat gaaaaattta tcccagctgt tgctaaattt ttcaaaggtg aacgtaaagt
    17221 atttttcgta gatttattaa ctgatttaaa ctttacatta aaatcttaca ttcaaggtca
    17281 agtaactgtc agtgttattt taggtatatt cttatatatc ggttattcaa ttattgactt
    17341 accatatatt cctttattag tactatttgc tggtgtcgca aacttaattc ctttcttagg
    17401 ttcatggtta tcatttgcac ctgcagcaat actcggtatt atagatagtc ctactacatt
    17461 tatatgggtt tgtatcatta cattaattgc acatcaactt gaaggtaata tcattacacc
    17521 aaatgtaatg ggtaaatctt taagtatcca tcctttaaca attatcgttg ttattttagc
    17581 agcaggagat ttaggtggct ttacattagt tctgattgca gtgccattat atgctgtact
    17641 taaaacggtt gttagtaata ttttcaaata ccgccaacgc attattgata aagcaaacag
    17701 taatgttaag gactaattct gtggatgtct tttaagaata taagatacta tcgcatcaaa
    17761 agttgaaact acagcttttg aggcgatttt tttgtgcata aaaaatcagt caaatgaaat
    17821 atcaaataat tttccatcag tccgattatt ataaaagcaa aaaagctttg ctcacatata
    17881 taataacgtg aacaaagctg ttgaatgata ttatttaatt gcgtggaatc cgctatctac
    17941 atgaatattt tcacctgtaa cgccacttga taaatcactt aataagtaag ccgcagtttt
    18001 acctacttct acttgatcaa cattacgttt taaaggtgca cgctcttcga tttctttaag
    18061 aattgtattg aaaccaccca caccttttgc acttaatgta cggattggac cagctgaaat
    18121 tgcattaacg cgaatattat ctggacctaa gtctaatgct aaatatttaa catttgcttc
    18181 taagctcgct ttagcaacac ccatcacatt atagttttga actgcgaatt cgccacctaa
    18241 atatgttgtt gcaacaatgc taccaccttc tggcattaat tttttagctt catgagccac
    18301 aattgttaat gagtaagaac taatgtcttg agctaacaag aagccttcac gtgaagtttc
    18361 agaaaagcgt ccgcgtaagt cttccatatt agcaaatgcg attgaatgat atacaccatc
    18421 aatattgcca acatctttac caatttgctc aaaaccatta ataacctctt catcgctttg
    18481 aacatcaatt tgatataagt gcgcttctgg ttgatttaat tgttctaata atttttcaag
    18541 ctctttacgg ctacgttctt tacggtaagt aaatactaat ttagcaccta attgatctaa
    18601 aactttagcg acaccaaaag caatactacg cttattagcg attcccatga tgacatatgt
    18661 tttgttttca agatttaaca tgtaagataa ctccttataa taattaattt aacggataga
    18721 atatctaatc ccagttttaa ctaacttttt attcgtcaat acattttaac actattatga
    18781 ctttttaata aagttattta aacgtattac aacgataaat gtttgattta gtcctttctg
    18841 tatcaaactt gatttacgta aaaagcattt atttatcaaa atcatataca tactagcatt
    18901 ttaaaatcat taattttacg tcgtactaaa atacgtctgc attttaatat cattattgtt
    18961 aaaaataaaa ttctaactct tgcttcaatt catcaacgta ttctttagca ccggtaacaa
    19021 ttaagcgatc tccatatcgc aattgtgtat ctccatgcgg aacaatcgac tcattattac
    19081 ggataatacg cacgaagatg atgtctcctc cgaatgggaa attacgtaat tgaatatttt
    19141 catatttata atttaacatt tgaatttcat atagtgacgt ttcaacatta cttaataaat
    19201 tcaacatgtt aggtgtttca attaaacctt ttaataagat tttattactt aagtagctac
    19261 tgaaaatttc aatacctgaa tcaactaact ctgtatcgtc cgttgtgctt tcaagtctgc
    19321 aaatgacacg ctcaacttga tgtgctttgg ctaatttagc aacttttcgg ttaatatcat
    19381 cgtcattcgt agcacaaaca actatgtctc ggtcaaacag acctagtcgt tctaaaacat
    19441 cttgttcata atcagcaatt tctatcatcg tgatatcatc tgacaattga cgacgatcac
    19501 tcaagtcttt gcgataatat aatgtcacgt catataactg agatgttaaa ttttgcgcta
    19561 taggaatcgt taattgattt ttaccaatta aactaacttc aattttacgg ttaaactcat
    19621 ctggaactgg aaacagtttt ttgaaaataa tcggaacgaa tacacatgta atgactgcgc
    19681 ttaaaattaa tatacctgac gtttcagctg aaatagcatt taatctttct gaaattttgg
    19741 ctgcagctat cacgagcgat aatgttgatg ttaataaaaa tgctgatgca atcgttgttt
    19801 tcatatcaaa ccaacgtcga ataaacatga ctggaattaa ttttgaaatg ataaatgcaa
    19861 cgattaaaat cggtatgata attagtaatt tcggttcttt aattaatgaa ggtatgttta
    19921 aatctacacc aaccattata aagaaaatag gaataaagaa cccataacca aatgagtcta
    19981 acttttcaac catttcttca tctggattta ataatgaaac aacgacacct gctaagaatg
    20041 cacctaatat attttctgcg ccaactccct ctgctagggc tactaataat attattaatg
    20101 caaataccgc acgaataccg atttgcgtcg taccatccat caatttttgt aaaaatgaca
    20161 ttcttttaaa ttgaacacct aaaatatatg aaattgctgt gaaaacaact aatataccta
    20221 ttaaccatat tgtactgccg ccttgaccat tgattgcgcc atagaccgtt aataaaatca
    20281 tagttactaa gtccgcaagt actgctacta ataggataaa ttgccctata gttgttctca
    20341 taatattcat ttcttttaaa gttggaacaa ctacgcctaa ggaaatagtt gaaatgataa
    20401 tgaccattaa taacacatca tccactaatc caagccattt aaatacatac gctaaaagaa
    20461 tcgaaataat cataataaat gcaaatacag ttaacgctag attaagatgc cctggaattg
    20521 aggattcatc atcattttgt ccttgacgtg cgcgtttatc ttttttaaaa gctttaaaat
    20581 caatttctaa accacttaaa aacattaaaa agataaagcc taacgttgat aaaatattta
    20641 gaattgaatc cctttctact atatttagaa atgaatttcc aatcacaatc cccatcaaaa
    20701 tttctgcaac aacaactggc aagaaattaa tatttaatcg attgacaatt attggagtta
    20761 aaaacgctgc taaaacaaca ataactaaag ataaaaactc cataactcct ccttaaatta
    20821 agtaagccat aaatgatgta gctaaaccaa aataaatcaa catactaata atatcattaa
    20881 ttgttgtaat aaatggtcca ctagccactg ctggatcgat atttaattta ttcatcaata
    20941 atggaatcat cgaacctaca aacgtcccca ccgtcatcgc acaagttaaa cttcctgcaa
    21001 cgattaatgc taaaagtggc tgatgatata ttgcaacaat aattgtaaat aatattgttg
    21061 aacatacaac acccgataat acaccacttc ctgcttctct taatgcaatt ctaaatttac
    21121 tttgctcatt aatttcccct gtcgtaatgt tacgaactga aacggctaaa gattgtgtac
    21181 ctgaatttcc tgacatacca ctaataatag gaataaacgc tgcgagtagc gctacatttt
    21241 ctaatgtttt ttcgaatctc cctaaaattg tcgcagtaat cattcctaaa aatgttaaaa
    21301 taatcaacca tggtaaacgt tttaatgctg ttttaatgat tgaatcatta gtcgaatcga
    21361 tatctgatac cccggctaaa cgagagtagt cttcactagc ctcttcatcc ataacgtcta
    21421 aaatatcatc aatcgtgatg ataccaagca aatgttcttg gtaatctata acaggtacag
    21481 ccatgaaatc atagtctctc ataacttgag caacatcttc ttggtcgtct gctacattca
    21541 cactaatgac acgttcattc ataatatctt caatataagc atcattttca gctacaatta
    21601 aatctcttag cgataaaaca cctactaatt taccatcatc atcaacgaca aatataacat
    21661 atattgtttc tgcgtctggt gcttgcgctt tgaccaataa taatgcttct ttaacaggcg
    21721 tatgcgcttt aagtgataaa tactccgtcg tcataatacc gccggccgta tcctcatcat
    21781 agtgaagtaa tgctttgatt tcattcgcgt catctttatt catcaatgtt aaaagactag
    21841 caacttttgg tttagtcaac tcatttaaaa tatctactgc attgtcgtat gacatttctt
    21901 ctaatatgtg acttgcgtat gtcgcattca tcttatcaaa tagcaactca tattcatcgt
    21961 catcaatatc taattgatca aagaaatttg caacttcttc aggtgataaa tattgaaaaa
    22021 tcttttgtct attttcatcg gtagtatctt caaaatactc actttgttca tatgtgtgta
    22081 atgctagaaa ttcatctcta aattgatcaa tatcatcatt ttctaaatat tgatctaata
    22141 aagtttggtc gtataattcc tcttgaacac gctctttttc atctgtgttc attgacatac
    22201 ttacaccccc tgtgtgaatt agagttgatt gaataattta agtaattgct tataatcaat
    22261 tgtaatataa atattgttct tattgattgg atgaacaaaa tatatttgcg tacattgcag
    22321 tacttgacca tgaattttgt catgaaaacc atcatacaaa gaatctccca caattggatg
    22381 cccaatatgt tgaaaatgta cacgaatttg atgtgtacgt cccgtatgca aatgaacttt
    22441 gcataaactg tatttatcat tctgatttat tacttcataa gatgttttag cgtatttacc
    22501 atccgaggca acttctctag ttataatcct atcctttgac cgtctaatat tagcttcaat
    22561 aataccagat gtatgggttt taccatatac aaggcaagta tatatttttt tcaagtttac
    22621 tttagaaaat aaatgatgga tatgtccata tttagcgaat atcacaatac cagttgtatt
    22681 acgatctaga cgcgtaacaa tatgtgggtt aatattttca ccatgttcct gacaatgata
    22741 tagtacttgt tcgattaaac tttcatgagg atgttctctc gaaggcgtac aattttgatt
    22801 gtttggttta gtaactatga tgataaaagc atcttcatac aatacttcta gcttacgagc
    22861 ataaggtatt aaattaacac tcggtatttc tcgcggtaaa tgaatttcta atatatcatt
    22921 tgtcattaat tgcttacgca ctgtgactgg ttcatcatta acaattaaag cgccattatt
    22981 tttaatggcg ctcactgtct tcttagaaaa atcatgtcgt gctaaaaaag ttttaacagt
    23041 ttcttgttgt gatatatgat acttaaattt catcgttctt catcacttga tatgaaagaa
    23101 tcgtgcacac gtttccagaa tgggaatggt ctaaaacgtg caaacctcac tttttcattt
    23161 gctacacggt attgtattga attaacattt ttatgtttga tactaacatg atctatcgtc
    23221 attctaatgg tatcatgatt aactggtgat attaaacatg tatgatgctt tggtaataca
    23281 agtggtgatc ctaccgttct aaacacacga ttatttatcg aggcaatttc tgtaatttgc
    23341 attgcttcaa gtgaaggatg tatcagtgcg ccacctagcg ctttgttata agccgttgaa
    23401 cccgaaggtg ttgatacaca taatccatcg cctctaaatc gctcaaagtg tttccctctt
    23461 aagttaacat ccacaacaag tgttgagcca ttttcagttt tcatcgttgc ttcatttaat
    23521 gctaaatacc ttgtttcata gccgttgtcg ttgtatctca taataatttc aagcaatgga
    23581 tattcaatga cctgaaactc tgaattatta atttcgatga ttaatttttc aacttcatga
    23641 ggtaaccaat ccgcataaaa tcctaaatga cctgtatgaa ctccaacaaa tgccactttt
    23701 gataacatgt ggctatactg atggaatgct tgtagtaatg taccgtcacc accaactgaa
    23761 ataacaattt caggattttc actatcctca atcatgcgaa aatctttcat atagttcatc
    23821 attttatgct ttaaggcatt agacttggag tcacctttag ttaaaattgt ataacgcatg
    23881 atttataaca cctcgttaat cattttcatg ttttttagaa cgtttttgcg tgtaatattt
    23941 ttgagcttcc tgaatttcat ctttaatttc agacatctct tcatcaagta aatacgctgc
    24001 ttctgccgct ctttccaaac gatgttgaat ttcatccgga taagcaccat catatttata
    24061 tcgtaaagta tgttcaatcg ttgcccagaa attcattgct aatgtacgaa tctgaatctc
    24121 agccaatata aatttttggc cttgtaatgt ttcaattgga tattcaataa tgacatgata
    24181 cgagcggtaa ccactttctt tagtgttacg aatataatct cgttcttcaa ttactttaaa
    24241 atcttttctt tgtcttaaaa tattgacgac aacatcaata tcttcaacaa attggcacat
    24301 cattcttaaa ccagcgatat cgtacatttc ttctcttaac ctatcaaatg gtatttgtcg
    24361 tttgtttgcc ttatctataa tactagcgat tggtttaaca cgaccagtaa caaattctat
    24421 tggcgacgct tgttcaccaa cttcatattg tttgcgcatg cctttaagtt tcactttcaa
    24481 ctcatcaacc gcttgcttat aaggtgttaa gaactgatcc cattgattca tagtgcttca
    24541 ccccgcttta ttcaatgtcg aatgtttctt caacggttgc aacaaattcc ttaccataat
    24601 tattattccc ttttatgttg tctaaaatat aagtaagttc ttggtggaat tcagtcaatt
    24661 ctagttcatc attgataata atttttttag tcatgttttc atgcaacatc gtccatgttt
    24721 cttgtacgaa aatgagataa gcatacgctt taccatcatc taatgtatac gcaaaggcat
    24781 aattatcact atcaacgagc atttgtccta cttcagataa tcctttaata gaatcttctg
    24841 tataacaata aagtatgtca tctttaattt taatttcatt aatatataaa cgcatgctta
    24901 atcctcctct tattccataa cattttaaca caattcaata atacaaaaca cttacgaaca
    24961 ctatttttaa tggtcaaatt aacattgata atagataaag gaaattactt ttaaaattca
    25021 actttgtcat tcataatata gttaagataa attgaatttg atacgaggtg tatacatatc
    25081 atggcaacaa atcatgaaat agaatttaaa caaatgataa ctgcatcaat ctataataaa
    25141 ttgcaagaga aatattttaa agacagtgtg ctttttaagc aagtcaatta ctatattgat
    25201 acacccgatt tcaaattaaa ggaacatcgc tctgcactac gcataagagt taaagacaat
    25261 caatatgaaa tgactttaaa gacaccagca aaggttggtc tattagaata caactatatc
    25321 gtcgacatta aaccagagat gaacttgatt atttcaaacg acaatcttcc agatgacatt
    25381 cgtcaaatta tagttgaaca gtttggtgta aaagatacaa ctttatcaat acttggtgca
    25441 ttaacgacat atcgccaaga aactaaatat aaaggtgatt tactggtttt agataaaagt
    25501 gagtatttcg atactacgga ttatgagtta gagttcgaag ttaaagacta taatcaaggt
    25561 ttacaaaagt tccaatcttt attaaacgaa ctaaacctcg aacatcatca acctttaaat
    25621 aaagttcaac gttttttcaa gaaaaaagaa acactttcaa acaatataaa ttaaattggc
    25681 aaatatgcat atttctttca aatgtttaaa atcatgttat attagttata tattaactta
    25741 agttcgtaac agtaaatttt tgaaaatggg tgattaaatg acaacaacac catatgacat
    25801 cattggtaaa gaagcgttat acgatatgat tcattatttt tacacccttg tagaaaaaga
    25861 tgaacgactt aatcacctgt ttccaggaga ttttgcagaa acaagtcgta aacaaaaaca
    25921 atttttaaca cagtttttgg gcggtccaaa catttatacc gaagaacacg gacatcctat
    25981 gctaagaaaa agacatatag attttacaat cactgaattt gaaagagatg catggttaga
    26041 aaatatgcag acagcaatta atcgcgctgc gtttccacaa ggtgttggag attatttatt
    26101 tgagcgctta agattaactg ctaatcatat ggtgaattcc taaaattaat tgtaggtgaa
    26161 taaacatggc tggagaatta cgaataatgg aaaataagag tcgtgaagat atcaatctat
    26221 cacctgtaag taaaatcgaa atttattcat ttttcgatcc atttagctcc gattgcttca
    26281 aattatcagc aatcttatcc aaattaagaa ttgaatataa tcaatatata cgtatcagac
    26341 atatattaaa tccttcgtta aaagtattaa cgaaatgcca agctcaaagt acatccaact
    26401 ttgataacat cgccctagct tataaagcag ctgagttaca aggtcgtgta cgtgccgaac
    26461 gatttataca tttaatgcaa aatgaaatca tacctaaaag agatattatt acagaatcaa
    26521 tgatttgtga ctgtattcaa aatgcaggta ttgatttaga agtatttaaa gacgacttac
    26581 aaaaaagtaa actaaccgaa agcttgaaaa ttgatttgca tattgcaaga gaaatggaaa
    26641 tcgaacaagc cccttctctc gttttcttta gtgaagatgt tcatgaagaa ggtttaaaag
    26701 tcgaaggatt atacccatat cacatctata cttatataat taatgaattg atgggtaaac
    26761 ctatcgaaaa gaatcttcct cctaaattag aaacttatat acagcaacaa caacttgtaa
    26821 cgatggaaga attacttact atttatgaat ggccagaaaa acttttaaac aaagagttaa
    26881 agaagttagc cattcaacaa aaaattgaaa aacttaaata tccagatggc gatttctgga
    26941 aatctaaaat gcctaaaatc aaatcaaaat aaataaatta aagacctcgt acaccataaa
    27001 tgtaacgagg tctttttact gactcttaat tttgcaacat taaattatta gaacgtaatg
    27061 ttacacatcg atctaaatat attagtgata tttttcgaat ttaaccagtt cgaaaattat
    27121 acaaattata aatttggtac ataatagaca tatatgaatc gttaatgtat aaaaagtgca
    27181 tatttaatac atttttatac aacttcactt ccctcttatt aaaaaagaac ataaaaaaac
    27241 gtcgtgtctg aatacacgac gctaaacaaa ggggatggga gaaatttttc acttcaaaca
    27301 aaggggtatg tttgttatgt gattaatttc atgttcataa tataacatgc tatctctacc
    27361 atttcaacct tttaatagta cttaatttaa attgtcacaa tctgttcata atgtaagcga
    27421 atacaatagt actaagcttt cattaatttt tcaaaagcgt tcaatttttg ttcaaaaact
    27481 tcacaagctt gttcaattgg ttcaggtgtt gtcatatcta caccagcatt ttttaatatc
    27541 tcaattggat aatttgagct accttttttc aagaattcat taatatatct atctactgct
    27601 ggcttacctt ctgttaaaat ttgatgactt aagctttgag ctgcactgta accagttgcg
    27661 tattgatata cataataatt catatagaag tgtggaatac gtgaccattc cttactaata
    27721 tcttcatcag tttctacaga atcaccgaag tataatttat ttaatttggc atattcttca
    27781 ttcattctag ttggcgttaa tggttcacct gcttcttcaa ttgcatgaat tttatgctca
    27841 aattctgcga acattgtttg tcggaataat gtagctctga aacgttctaa ttcttggttt
    27901 aataataata agcgtttttc atcatcaaga tgtttatcca tataatcact taaaagtgct
    27961 tcgttacaag ttgatgcaac ttcagcgaca aaaatagtgt agtcactaga atttgacggt
    28021 tggaattttc tactgaagta actatgtgct gaatgcccaa attcatgaac taatgtgtat
    28081 aagtctgaaa tagtattaga ccagttaagt agaataaatg gattagttaa atgtgcacct
    28141 gatgaatagc cacctgaacg tttaccttta ttctcataga catcgaccca acgattgttt
    28201 aagccttctt taactacatt taaatattct tcacccattg gttctaatgc ttttaacatc
    28261 cactctttag cttcttcata aggcatttca aacttaatat ctttaattaa tggtgtatat
    28321 aaatcataca ttttcaagtc atctaaacct agcaattctt tgcgcaattc agtatatcta
    28381 tgtagcaatg gtaaatattt atgtacagtt tttactagat tgtcatatac
//
LOCUS       DANRDI010000018        27748 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.018, whole genome shotgun sequence.
ACCESSION   DANRDI010000018 DANRDI010000000
VERSION     DANRDI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.018"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(137..1405)
                     /gene="ilvA"
                     /locus_tag="PZ377_000600"
     CDS             complement(137..1405)
                     /gene="ilvA"
                     /locus_tag="PZ377_000600"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDM6357490.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(1420..1992)
                     /gene="leuD"
                     /locus_tag="PZ377_000601"
     CDS             complement(1420..1992)
                     /gene="leuD"
                     /locus_tag="PZ377_000601"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDM6357491.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            complement(1993..3363)
                     /gene="leuC"
                     /locus_tag="PZ377_000602"
     CDS             complement(1993..3363)
                     /gene="leuC"
                     /locus_tag="PZ377_000602"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDM6357492.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            complement(3377..4423)
                     /gene="leuB"
                     /locus_tag="PZ377_000603"
     CDS             complement(3377..4423)
                     /gene="leuB"
                     /locus_tag="PZ377_000603"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDM6357493.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            complement(4426..5955)
                     /locus_tag="PZ377_000604"
     CDS             complement(4426..5955)
                     /locus_tag="PZ377_000604"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDM6357494.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            complement(5985..6989)
                     /gene="ilvC"
                     /locus_tag="PZ377_000605"
     CDS             complement(5985..6989)
                     /gene="ilvC"
                     /locus_tag="PZ377_000605"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDM6357495.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            complement(7126..7380)
                     /locus_tag="PZ377_000606"
     CDS             complement(7126..7380)
                     /locus_tag="PZ377_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDM6357496.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            complement(7380..9149)
                     /gene="ilvB"
                     /locus_tag="PZ377_000607"
     CDS             complement(7380..9149)
                     /gene="ilvB"
                     /locus_tag="PZ377_000607"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDM6357497.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            complement(9177..10865)
                     /gene="ilvD"
                     /locus_tag="PZ377_000608"
     CDS             complement(9177..10865)
                     /gene="ilvD"
                     /locus_tag="PZ377_000608"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDM6357498.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            complement(11183..11338)
                     /locus_tag="PZ377_000609"
     CDS             complement(11183..11338)
                     /locus_tag="PZ377_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357499.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKFKIF
                     VDILKQI"
     gene            11376..11837
                     /gene="tsaE"
                     /locus_tag="PZ377_000610"
     CDS             11376..11837
                     /gene="tsaE"
                     /locus_tag="PZ377_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDM6357500.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            11818..12480
                     /gene="tsaB"
                     /locus_tag="PZ377_000611"
     CDS             11818..12480
                     /gene="tsaB"
                     /locus_tag="PZ377_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDM6357501.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            12453..12917
                     /gene="rimI"
                     /locus_tag="PZ377_000612"
     CDS             12453..12917
                     /gene="rimI"
                     /locus_tag="PZ377_000612"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDM6357502.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            12910..13935
                     /gene="tsaD"
                     /locus_tag="PZ377_000613"
     CDS             12910..13935
                     /gene="tsaD"
                     /locus_tag="PZ377_000613"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDM6357503.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(14037..15647)
                     /locus_tag="PZ377_000614"
     CDS             complement(14037..15647)
                     /locus_tag="PZ377_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDM6357504.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            15736..15864
                     /locus_tag="PZ377_000615"
     CDS             15736..15864
                     /locus_tag="PZ377_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357505.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(16008..17936)
                     /gene="abc-f"
                     /locus_tag="PZ377_000616"
     CDS             complement(16008..17936)
                     /gene="abc-f"
                     /locus_tag="PZ377_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDM6357506.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDFGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            18189..18824
                     /locus_tag="PZ377_000617"
     CDS             18189..18824
                     /locus_tag="PZ377_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDM6357507.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            19128..20207
                     /locus_tag="PZ377_000618"
     CDS             19128..20207
                     /locus_tag="PZ377_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDM6357508.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            20267..20491
                     /locus_tag="PZ377_000619"
     CDS             20267..20491
                     /locus_tag="PZ377_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDM6357509.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            20699..21949
                     /locus_tag="PZ377_000620"
     CDS             20699..21949
                     /locus_tag="PZ377_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDM6357510.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            22132..23082
                     /locus_tag="PZ377_000621"
     CDS             22132..23082
                     /locus_tag="PZ377_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDM6357511.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            23231..24715
                     /locus_tag="PZ377_000622"
     CDS             23231..24715
                     /locus_tag="PZ377_000622"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDM6357512.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            24712..25671
                     /locus_tag="PZ377_000623"
     CDS             24712..25671
                     /locus_tag="PZ377_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDM6357513.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(25859..25966)
                     /locus_tag="PZ377_000624"
     CDS             complement(25859..25966)
                     /locus_tag="PZ377_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357514.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(26038..26754)
                     /locus_tag="PZ377_000625"
     CDS             complement(26038..26754)
                     /locus_tag="PZ377_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6357515.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(26773..>27748)
                     /locus_tag="PZ377_000626"
     CDS             complement(26773..>27748)
                     /locus_tag="PZ377_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDM6357516.1"
                     /translation="SDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGF
                     LIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEM
                     KYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMPGLRDYFNK
                     NIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNFPISIEIPDEVSSINL
                     NMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELF
                     QESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         9037 a         4496 c         4804 g         9411 t
ORIGIN      
        1 caaacacaaa gaaagatggc ttggcgaagt gaaaacgttt gaatctgacg aaacgagaaa
       61 tgtaaagtat aataaaaagc agtcataaga tgatttcaat tagaaatcaa tttatgactg
      121 tttttcttac tatgtgttaa attaacaatg aatataacat cttattttca ttaatataaa
      181 tattggaagg atcgaaatga tttacacgtt gtttgagttg tattaaatca tcatgatctt
      241 taagttgaat accaataatg acagtacctg tattttgaga agattttttt aagtattcaa
      301 atttagtaat atcgtcttga ggtcctaata cgtcatttac aaattctctc aatgcacctg
      361 gacgttgagg gaaatttaag ataaagtaat gcttcatttc ttcgtatagt aatgaacgtt
      421 cttcaatttc tttcattcga ttaatatcat tattaccacc actaatgaca caaaccactg
      481 ttttaccttt aatatgatct ttatagtttt caagcgcact tacacttaat gcgccagcag
      541 gttctgctac aattgcttgt tttgaataca tatctaaaat cgtagaacaa actgcacctt
      601 catctacttg aacgtaatca tctacatttt cttttgcaat ttcaaatgta atatcgccaa
      661 ctctagctac agatgcaccg tccacaaatt tatcgatatt aggcaatgtg actacctgat
      721 tatttaccac aacagattca tacatactac ttgcacctga aggttcaaca cctataattt
      781 tcgtggtagg tgaataggtt ttaaagtaag tactaatacc tgaaattaaa ccgccaccac
      841 caattgcggc aaatagataa tcaaagttaa cattgtcaga ctttgcttgt tctagcattt
      901 ctttagcaag cgtaccttgt ccagaaattg tatgaacatt attgaatgga tctataaagt
      961 tcatttgatg ttcacttgta taagttaaag cttcagctaa acagtgatca aatgtatcac
     1021 cagtgagtac aacttcaacg ttactatttc caaagaactt tacttgattt accttttgta
     1081 aaggtgtagt gactggcata aagataacag cgtttaaatt aagtttttta gctgtatagg
     1141 caacaccttg agcatgatta cctgcacttg cacatgtaat acctttactt ttagcttcat
     1201 ctgataaaac agaaatagcg ttgtaagcac ctcttaattt aaaagaacgt acccattgta
     1261 aatcttctcg ttttaaatag actttacaat catacttttg agataagtaa tggtctaatt
     1321 gtaaaggtgt ttctttgaca atatctttaa gtcttaaaaa tgcctcatcg atatctttcg
     1381 tagaaactgt tgttttgact gtcataatat tcaactccct taaagtgatt tttcatattt
     1441 ttctattaat gattcatatt gtagggtgat tgcaatgtca tctaagccat ttacaagttt
     1501 attcttccaa gtttcatcaa tttcaaaatg gaaacgcttg tctggtgatg acacagtttg
     1561 atttggtaaa tcgacctcaa tttcaacata ttgtgcaaga tgttcacggg cacttttttc
     1621 taaaacgata ggcaacatcg catttttagt gcaattcata taaaatatgt cactgaaact
     1681 tcctgcaata ataatatgaa aaccatagtc cttaagagcc caagcagcat gttcacgact
     1741 tgaaccacat ccaaaattat ctccagtaat taaaatagaa gcccctttat attgtggttt
     1801 gttaggattg aaatcaggat tatctgaacc atcaggtaag taccgccatt catcaaaagc
     1861 aaatggacca aagccacttt ttgaaatacg ctttaagtgt accttaggaa tgatttggtc
     1921 tgtatcgata ttgtcgttga agagagggac tattttacct ttatatgttg taataggttt
     1981 gattgctgcc atttaaacaa ccacctttct tacgtccaca aatttaccat gaatagctgc
     2041 tgctgctgcc atagcagggg atactaaatg tgttcttgca cctttgcctt gtcgtccttc
     2101 aaagtttcga ttacttgtag atgcacaatg tacgccctca ggtacttggt caggattcat
     2161 gcctaaacac attgaacatc ctggttcacg ccattcaaat cctgcatttt taaagatagt
     2221 atctagacct aatttttctg cttctttttt tactgtacga gaacctggta cgacaatagc
     2281 tgtaatattt ggatgaactt tatttccttt aacaatatga ctagcttcaa tcaaatctga
     2341 tagtctagca tttgtacatg aaccgagaaa aacataccct aagtcgatgt cttcagcttt
     2401 ttgacctggt tctaacccca tataatcata cgcacgttga tcgttgatat cattgatttc
     2461 agggaatggt tcactgaaat taacacccat ttcaggatta gttccccatg tcacttgtgg
     2521 ttctaatgtt gaaacatcaa gttcaattac acgatcaaat atcgcgtcgt catcagaata
     2581 tagctcacgc cacttatcaa ctgatttagc gaagttatcg gcaaatggtc tccctttaac
     2641 atattcaaat gttatatcat caggttggat tatgccgtat ttggcaccac cttcgatagc
     2701 catgttacaa atagtcattc gaccatccat tgaaaggttt ttaattgttt cgccagtaaa
     2761 ttccaaagca tagcctgtac caaagtcaac accatacgtt ttaattaaat gcagaataat
     2821 gtccttagca tagacgcctg ttggtaaggt accattaata tcgattttta agtttttggg
     2881 ttttgtttgc catagcgttt gagttgcgaa aacatgttca acttcacttg ttccaattcc
     2941 aaatgcaata gcaccaaatg ctccatgtgt tgctgtgtga gagtcaccac aaacgattgt
     3001 cttgccaggc tgtgtaagtc ctgtctcagg tcctaccatg tgaacaatac cttgttcatc
     3061 agaacccata tcaaaaatat gcaccccaaa atctatggcg tttttttgta atgttgtgat
     3121 ttgtttgttt gcaatttcat ctttaatatt gaatatatca atagtaggaa cattgtgatc
     3181 gagtgttgca aatgttaaat ctgggcgtct taattttctg ttttgaagcc taagtccttc
     3241 aaatgcttga ggagaagtaa cttcatgtat aaggtgtaaa tcaatgtata atagttgcgg
     3301 ttcgcccaat ttcccgtata acacatgtct gttccacacc ttgtcaaata atgtttgacc
     3361 catttacatc tcccccttag tgattcaatt tttgagatag aatttcgaaa atatcagtag
     3421 tattcaattt gccgcctaaa tctgctgtcg tttgcccatg ttcaatcatg ctataaatat
     3481 gttgttctaa ttcatctgca gcatctggtt gatttaagct ttcacgtaaa cacatcgcta
     3541 aagatagaat cattccaaat ggattggcaa cgtttttacc tgcaatatct ggtgctgatc
     3601 catgaatagg ctcatacaat cttggaccat cgttactaaa actagcagaa ggtgataaac
     3661 caagtgaacc aggaatcact gaagcttcat cacttaaaat atcgccaaat aagttttcac
     3721 atacgatgac gtcaaattgt tttggatttg tgattaaatg catactacaa gcatcaacaa
     3781 gtaagtgatt tactgttact tctggatata attgacttac ttcatttacg actttgcgcc
     3841 acaatttact agaagctaat acattttctt tatcaactga tgttagtttt cctcgtcttg
     3901 aagcggccaa tttaaatgct acgtgaacaa tgcgttctat ttcttctctt gtataagtaa
     3961 gagaatctaa ggcctcgtga ttattaaaat gtctaggttc tccaaaataa ataccacttg
     4021 tcaattcacg gactataact aaatctgtgc cttcaacgcg ttcttccttt aaaggtgata
     4081 aagaactagc gcctttgaca acggtagtgg ggcgtatatt tacaaataaa tttaaggatt
     4141 tacgcaattt taataatcct tgttctggtc gattgttagg atctgtccat ttaggtccac
     4201 cgattgcacc cagtaaaata gcatctgctc ttttacacgc atttaaggtt ttctcagtta
     4261 aaggctcgcc gaatgtatca atagaggcac caccaaattc gtggtgctct atttgataat
     4321 taaagttata tttattactt ataatttcaa gcaatgatag agatccgttc aaaatttctg
     4381 gaccgattcc atcaccaggt agggcaacaa tgttataagt cataattaat tacctacctt
     4441 ctcaacattt tcagctgcaa atttagcatg tgcttctacg tatgctttac aagaggcttg
     4501 taaaatatca tgatcaatac caaagccatt gacagtctta ccttcaatca ataaatttac
     4561 atgtacttct gcttgggcat cagtaccttc agtgacagaa ttaatacgat aatcaattaa
     4621 ttctgtttct ttctggaaaa tacgatcaac tgcattgtaa attgctacga ttgaaccagt
     4681 accaatactt gaatcctggt aaatatgacc ctctttatct ttaacaacaa caacagcact
     4741 ttgaaggccg ctagagacat attgtagttg cagtgtttcc aatttataaa gtgcttgatg
     4801 ctcatgctca gaaccttgaa taatcgcatg aatatctcta tctgaaacag atttcttttt
     4861 gtccgcaatg gccttgaatt gtttaaataa atctatttgc gcttctttat caatgtcata
     4921 acctaatgct tttaacttct ctgagaaggc gtgtttacca gataattttc ctaatggaag
     4981 ttcagtcgtg cttacaccaa caagttgagg tgtcataatt tcatatgttt cacgatgttt
     5041 taatacgcca tcttggtgaa tacctgattc atgactaaat gcattttggc caacaattgc
     5101 tttatttcta ggcactcgaa tacctgcata tcttgaaatt aaatccgatg tttttttagt
     5161 ttcttcgaga ttaagagcag tttgagcacc ataatgatca tttcgaacgt atagtgcaag
     5221 cgcgacttct tcaagtgctg cattacctgc tcgttcacca ataccattta cagtgccttc
     5281 aattcgtctc gcaccgcctt caattgcagc taaactattt gaaacagcca ttcctaaatc
     5341 gtcatggcaa tgagcactat aaataatttc atttgaagat gttacagatt ctgttaaggt
     5401 tttgaaaata tgtgcatatt catcatggta actgtagccg actgtatcag gaatattaat
     5461 aactgtagct ccagcgtcaa cggcagtttg tacacatttc actaagaatg gtaattcagt
     5521 acgcgttgca tcttcaggtg aaaattgaac aacgtcaaat aattgtttcg cgtatgtgac
     5581 atgttcttta atagatgcta aaacgtcttc ttgagacatt ttaagtttat gttcaagatg
     5641 aataggtgat gttgctataa aaacatgcac gaccggcttc gctgcatctt ttgttgcttc
     5701 atatacagca tcgatgtcag attttttaca tctagctaaa ccacatacag ccgttgttgt
     5761 taatgtttgt gcaattgctt gaacagattt aaagctacct gtacttgaag caggaaatcc
     5821 agcttcaata acatctacac cccatttttc taattgcaat gcaatacgca agcgttcatc
     5881 aaaagtaaaa ttcactcctg gtgtttgttc accgtctctt agtgtcgtat caaaaatttg
     5941 aatatgacta ctcatattta acaactcctc attgtaggtc tatcttattt ttcaatgctt
     6001 ttagatttaa taaaaggcat catttcgcgt aattcacgac caactttttc aatttgatga
     6061 ccatgttgtt cttcgcgtaa tttataaaat tctttgaatc catttttatt gtcttcgata
     6121 aagcgattac tgaagttacc attttggata tcagttaata cagctttcat attttcttta
     6181 acatctggtg tgataacacg tggtcctgaa acatagtcac caaattcagc agtatttgaa
     6241 attgagtaac gtacattttc cataccgcct tcatacatca aatcaacgat taatttcatt
     6301 tcatgtaata cttcaaaata agctaattct ggttgataac ccgcttctac taatgtttca
     6361 aagccacttt gaattaattt cgatacaccg ccgcaaagta ctgcttgttc accaaataaa
     6421 tctgtctcag tttcttcttt aaatgttgtt tcaataacac ctgcacgagt tgcaccaata
     6481 ccttttgcat aacttaaagc aatattacgt gcttgacctg aagcgccttg ttgaatacca
     6541 aatagtgatg gtacagcaga accttcaaca aatgtacgtc taactaaatg acccggtcct
     6601 ttaggagcta ctaaaaatac atcaacatca gctggtggtt gaataacacc aaaatgaatg
     6661 ttaaagccat gagcaaatgc aagcgcatta tgtttttcta aatttggttc aatttcgttt
     6721 ttgtatacat caccttgaat ttcatcaggt aatagcacca taattacatc agcttgctta
     6781 actgcttctg caacagggaa cacatcaaat ccatcttctt tagctttgtc aaaagaacga
     6841 cctgggcgaa tgccgatgac tacatcatat ccattgtctt ttaagttttg tgcatgcgcg
     6901 tgaccttgtg atccataacc tactactgca atttttttgc cttgtaaagc gtccgttttt
     6961 acatcttgat cataataaac tgttgtcata ataaattcct ccaaataatt aaatattttg
     7021 tgtttcgaaa attctgaaat ataactttgt aatagtggca gatgttaact agcagcctat
     7081 gatatatcta taactaatta gcgcatcatt gcctttgtct taaaattaag cttcgttatc
     7141 aacaaggtcg tagcattcaa ccgttaaaac attaatttgt tgttttaatt ttttaattaa
     7201 tatatgaagt gatgtatcat cttgaatatc gacttgaatt tccatgttag aaatcccagg
     7261 ttgttcagaa tgtgtaacat gtaatgtatc gatattatat tgtaggcgaa caaaagcact
     7321 tgtaattcga tttagcgtgc tgacttgatc cgcaacttgt aatttaagaa ttcttgtcat
     7381 tataagccct ccatttcatg atttgatttg ccactcggaa ccattggggt tacagcttca
     7441 gtaggggaaa tacgaacctc aattaaagct ggtccttgat aagcaaacgc tgcatctaat
     7501 tgttcttcca gttgttctgg cttatcgatt aagaaacctt tgacgccata tgcttctgcc
     7561 attttcataa aatcaggttg accattaaat actgagtgtg agaagcgttg attaaagaac
     7621 ttatcttgcc attgtttaac catacctaat gttccattat tgattagtac gattttgaca
     7681 tctaaaccat attcgggtaa aagtgccatt tcttggtttg tcatttggaa accaccgtca
     7741 ccgacgaaac atacgactgt tttatcagga ttagctaatt tggcaccaat tgacgaagga
     7801 ataccgaatc ccattgttcc taaaccaccg cttgtaaccc attgtccgtg atttttaaat
     7861 ggataaaatt gagctgccca catttgatgt tgtcccacgt ctgtagtaac aattgcttca
     7921 ccatttgtaa ttttgccgat atattcgatt gtttgttgtg gcttacaaaa tacttgatct
     7981 tcttcaccaa gtttaaatgg gtgtttctgc ttattatttt gacaatgttt aacccagtca
     8041 ctgtgttcta tagtctcaac atttttatca tttaaacatt ctaaaaatct tttacagtct
     8101 gcaataatac ctaaatctac atgaataact ttattgattt ctgaaggatc aatatctaca
     8161 tgtacaattt tggcgttagg tgcaaaggca tcaggtttgc ttgctaatct atcatcgaag
     8221 cggctaccta aattaatgag taaatcacac tcagttaatg ccatgttact agcataagaa
     8281 ccatgcattc ctcccatacc taaaaatagt gtatcctcat aaggtacagc acctagacct
     8341 agtaaagtag taactgttgg gatttgatgt ttattaacaa actgtgttaa taattgattt
     8401 gattttgatt gattaatacc tgcgccggct aatacgacag gctttttcgc ttcttttaaa
     8461 agtgagatga aagtgtcaat gtctttattt tctggttctg taacaacttc ataacctgga
     8521 atattgattt cgtcgcataa atccacattt gtagctaaaa cacccatatc ttttggaaaa
     8581 tcaatcacta caggaccttt gcgtccagaa ttagctacat ggaaagcttc gtgtacgatt
     8641 ttagggatat cttcaacacg tttcacttga taattttgtt ttgtaattgg tgaagtcata
     8701 gatagaatat ccgcttcttg gaatgcatct ttaccaatgc ctggtgtagc aacttgtcca
     8761 gtgaatacaa ctagaggtaa agagtcgcaa tgtgcatccg taatacctgt cattacatta
     8821 gttgcacctg gaccgcttgt aactacaacg acgccagttt taccagatac acgtgcataa
     8881 ccttctgcag catgaacagc accttgttcg tgtcttgcta aaatatgttt gattttacca
     8941 tcataaaacg tgtcatataa aggtagtacg gcaccaccag gataaccgaa taaataatcc
     9001 acattttctt taagtagagc ttctactagc acttctgatc ctgaacgcat atcatttaaa
     9061 gttttttcat ttagttgttc aggttcaagt gattcagcca tttttaaagg gtcaatattt
     9121 tggtttactt catgttgagt tttagacatt ttaaccatct ccaatataaa aataaattaa
     9181 attaaattct cagggacttg catgacgcca cctgtatttg cgctagttac tagggcagta
     9241 taacgagcta gataacctgt ttttactttc gctttaaatg gtgttaaaga ttctcggcga
     9301 cgcgctagaa catcttcagg ctggtttacg tttaatgtac gatttgttaa atcaatagta
     9361 atctcatcac catcttcaat taaggcaatt ggtccaccag atgcagcttc aggggaaata
     9421 tgaccaactg caatacctct tgtggcaccg gaaaaacgcc catcagtaat taatgcaaca
     9481 tctttaccta agccgcgacc aacaatagag gaagtaggtg ctaacatttc aggcatacct
     9541 ggtccacctt taggtccttc atatctaatg acaacgacgt ggcctgcacg aacggtacga
     9601 ttgtctattg cttcaacagc ttcatcatgc gaattgaaac aaattgcttt cccagtaaat
     9661 gttttgatag atggatcaac gccgccaact ttaataactg cgcctttagg ggcgatatta
     9721 ccaaataaga tagataaacc gccttgtgca tcatatggtg catcaagagg gtgaatgaca
     9781 tcaaagttct taatttcttt gccttcgtta ttttcacgta acgttttgcc agtaactgtg
     9841 attctatctg ggtgtaacgt gccatctttc ttcatcaatt cattaataat tgctgggacg
     9901 ccaccagctt catgcacatc atgcattgaa tacgatgaac taggtgctat ttttgataaa
     9961 tatggcgtgc gtttggcaat agcattaatg cgctctaagt cataatcaat accagcttca
    10021 ttggcaatgg ctaacgtatg cagtaccgtg tttgttgaac cacccatagc catatcaagt
    10081 gcaaatgcat catcaattgc ttcgcgagta acgatatcac gtggttttaa atcattttta
    10141 atattttcaa ctaatttaaa agctgcttgg cgaatcattt ctcgtcgctg atcactgact
    10201 gcaagtgcag taccgttgta tggtaatgtt agacctaaaa cttccatcaa acagttcatt
    10261 gaatttgcag taaacatccc agcacatgaa ccacaagtag ggcaggcatt ttgttccata
    10321 tctaaaaatt cttctttaga aatcgatcct tctttaaatg cgccgactgc ttcaaacatt
    10381 gatgaaagtg ttaatgcttt tccatgtgca gataagccag ctttcattgg tccaccagag
    10441 caaaagatag ctggtacgtt tgtcctcatg gctgctaaaa tcataccggg tgtaatcttg
    10501 tcacaattag gaatgtaaaa tacgccgtca aaccaatgag cgttaattac agtttcagct
    10561 gcatctgcaa taatttcacg tgatggtaga gaatatcgca ttccgatatg tcccatagct
    10621 attccatcat caacaccaat tgtattgaat tcaaatggaa tggcaccggc ttctctaatt
    10681 gcttctttag ctatatctgc aagctctctc aagtgaacat gtccaggaac aatatcaata
    10741 taagagttac aaatagctac aaatggtttg ttcatatcag ttggactttt tagcgcgccc
    10801 gtggcatgta aaagacttct tgctggtgct tggtgatctc cttttttgat catgtcgctt
    10861 cgcatagtaa attcccccgt aaattttaat gattaatcat gttttatagt aataaaaata
    10921 agtaattgaa agataaaaca taaaaaaacg tcccaatatt attgggacgt ttacaagtcc
    10981 cattcaaaca gaaataggac ttaaagcgtt tagtaaatat ttactaacgt tcgagtccta
    11041 aagagtataa taaaataata atatttgaag ttgtcggttg atgttcagta tattgattaa
    11101 gcattttatt atactccttc cgtgaatggt atatatcact ttaatagtta gaattgtcta
    11161 aaaatttgat aaatttaatt tactatatct gctttagtat gtcaacaaat attttaaatt
    11221 ttcttacaat taactttgaa tagataatct gtttcaaaga taagaaattt aagttgaaat
    11281 ttgttacaat ataaaggtta aatataaagt tagatacgat tgaacaatgg ataatcattt
    11341 ttatgaatga aaagcataat ataggagaat caacattgat aaagataaat aatttagatg
    11401 aaatgaatca atttgctata tttttagttg agcaattgaa aagtggtgat ttgattttac
    11461 ttaacggaga tttaggagca ggtaaaacaa cgttaacgca atttatagga aaagctcttg
    11521 gtgtaagacg tacgattaat tccccgacat ttaacatcat taaatcatat aggggtaaaa
    11581 atttaaaatt gcatcatatg gattgttatc gcttagaaga ttctgatgaa gatttaggat
    11641 ttgatgaatt tttcgaagat caggcaatta ctgttattga atggagtcaa tttataaaag
    11701 atttacttcc agcgacgcat ttatctatta acatttcaac aatatctgaa aatacaagac
    11761 aaattgagtt gttcgcgcaa ggagaacatt atgaacaaat taaggaggca attatccatg
    11821 aattcgctgc tcattgatac atcgaatcaa ccattatcag tagcattgat gcaaaacgat
    11881 gaagttttag ctgaaataac tacggattct aaacaaaatc attcagtgca attaatgcct
    11941 gcgattagtc agctatttga gcagagcaaa atagctaaac agcaattaga tgcgattatt
    12001 gttgctgaag gtcctggttc atatactggc ttacgtatcg gtgttactgt tgctaaaaca
    12061 ttagcgtatg cgttagatgt taaattatat ggtgtttcat cattaaaggc attggcagca
    12121 acaatcgatc atactgataa attattggtt cctgtgtttg atgcaagacg acaagcagtt
    12181 tatacaggca tattccagtg gcaaaatggt caattagtga ctattttaga agatcaatat
    12241 atgtcgatag aggcattgcg agctttttta gaagatttaa accaaccatt tatttatatt
    12301 gggaaagata ctgtaaaatt acaagatgaa ttacaaggtg aagtgattgc gcagctacca
    12361 aatgcatcag tcatgtatca attgctagat aaaccttcag acgtacatac atttactcct
    12421 aaataccata aattagctga ggcggaacga aattggatca acagtcaaaa gaacaattaa
    12481 acattagaga gatgactaaa gaagatgtgc cacaagtctt tgatcttgag cgtcggagtt
    12541 tcaatgacag ttcttggaca attgacgcat tttatcatga aatagagcaa aataactttg
    12601 cgaaatattt tgtcttagaa tttgaacaac aaattattgg ttatttaggt ttatggattg
    12661 ttatcgatca agctcaaatt acaacagttg caatcgatga taattataga ggctatggtt
    12721 taggtcaaat gcttttaaaa tacggaataa actatgcaag tcacacttgt gatgtgatga
    12781 gtttagaagt aagagttaat aataaagtgg cacaacatgt atatgaaaat ttaggatttc
    12841 aatatggtgg taaacgtaaa aattattatg gtgaaggtga ggacgcaatg gttatgtggg
    12901 tgaatttaaa tgactaaaga tatattaata ctagctgttg aaacaagttg tgatgaaaca
    12961 agcgttagtg ttataaaaaa tggcagagat attttatcaa atacagtttt aagtcagatt
    13021 gaaagtcata aacgatttgg cggtgtcgtt cccgaagtgg caagtagaca tcacgttgaa
    13081 ggtataacag caacaataaa cgaggctcta ggggatgccg atgtatcaat agaagatatt
    13141 gatgccatag cggttacaga aggccctgga ctaattggtg cgttactaat aggtgttaat
    13201 gcagccaaag cattggcatt tgcttacgat aagccactta ttcctgttca tcatattgca
    13261 ggacatatat atgctaatca catagaagag ccattaacat tcccgctaat tgcacttatt
    13321 gtttcaggtg gacatactga attagtttat atgaaggatc atttatcatt tgaagtcatt
    13381 ggtgaaacac gagatgacgc agtaggtgag gcttatgata aagtggcacg aacaattggt
    13441 ttaaattatc caggtggtcc acaagttgat cggttggctg ctgaaggtga agatacttat
    13501 tcattccctc gtgtttggtt ggataaagat agttatgatt ttagttttag tgggttgaaa
    13561 agtgccgtaa tcaatcaact tcacaatcaa cgacaaaaaa atattccaat cattgaagct
    13621 aacgtagcaa cgagctttca aaacagtgtt gtagaggtgc tcacgtttaa agctattcaa
    13681 gcttgtaaag aatatggtgt tcagcgatta attgttgctg gtggcgtggc gagtaataaa
    13741 ggattacgtc aatctttagc ggatcaatgc aaagtcaatg acattcaatt aactatccca
    13801 agtcctaaat tatgcacaga taatgctgca atgataggcg ttgccggcca ctatttgtat
    13861 cagcaaggtc gatttgctga tttagcatta aatgggcaca gcaatataga tttagaagag
    13921 tattctgcag aataaaataa acgtcagtta ttaacatgat tactatgaac attattgaaa
    13981 ctgttcatag tcttctgtca tgtgatagct gacgttttta tgtttaaagt taaatgctaa
    14041 attttcggga cattatcttt tgctcgttca tatatttttg ctggaaatga agtgattttt
    14101 aataattcga tggcattacg tgtatttgct ttgccaggct taattttgta atcaaaatgt
    14161 atgttattat tttctattac ctcattgaaa tggtaatttt cataacgttg ttttaagagt
    14221 tcagctaact caatatcatg tgttgctgca ataactctaa agttagattt ttcatgtaaa
    14281 aatgatagta ctgattctga agcggcaatt cgttcagttg tgttggtacc tttaaaaatt
    14341 tcatctataa agcagtatat tttttgatta tctggaattt caacaatacg cttaatagat
    14401 tttaattcag ccataaaata actatcgcct gataacacat catcggcatt tgccattgac
    14461 gtgaacacaa ttccaggttg atatacaaat ttgcttgcgg taactgtttg tatagccgat
    14521 gcaagaataa tgtttattgc tatggatttc ataaatgttg atttacccga agcattagac
    14581 ccagttaaca gtatattttg tgatagtgag aaatcattgg ctactgcatc tgctatgagt
    14641 ggatgtgtta actcagagaa tactatgcca tcatttgaat catcaatttg tggttcagta
    14701 taacattcta gcgttctacg atacattgct aatgcataat gattatctaa ttcagctatg
    14761 taatcgaaac aatttttaag ctcattcata tgtgttgtat agcttttttg aattgaatga
    14821 aataaaacat aatcaagcat gaaaataagt ttaactagtt taattaccat agctccgata
    14881 tcttcatcat taacttcagc taaaacactc gttaaatgac gtgctgttct aaattgctta
    14941 aaattgactt caggttgtgg agcatgttta atttttgaga tagtatagcc ttgttttaaa
    15001 acattggatg cataaaagat agattttaag tcatcctcat atgtacgctt taaagttgct
    15061 gataaaatga tattaaatag gcaactcata agaaataata aaataccaac ttgtgaatta
    15121 ataaatataa ctgcaaatga gataactggt aaaaacgggc atagaaacaa tatattttga
    15181 cgttttaccg gtgtgatttg gtctggaaat gttggataaa cagttttacc aatcaaagct
    15241 aaatgatatg ttacgttttt tctaaattca gcattatcat taaataaact aagtaatgat
    15301 ttgttcgtaa ttgaaagcat tccacgtaaa caggcatata gcttcatttc accaatagct
    15361 gttaaattat aattcatctt atgaaagatt gcattcatat ttaagtcgga ccaagtttta
    15421 tcatcaacta atgaatgtga ttcaaatttt tccttgtaga gcttatactc atagtcgaaa
    15481 tgtgagttcg gtctgataaa agcttctaat ggagaaatcg tttgccataa tgtttccata
    15541 gattttaata gacgttgacg actcatgtat cgtccaacaa ttcctattac agatgtaagt
    15601 agaataattg ctataacaaa tattaaaaat gtttgatttg tgctcataag tatcccctta
    15661 tttattttta ataataataa aaatatactt caatattatt attatttcat aaagaaaatg
    15721 tcttaacaat gaattttgat aaaaacaatg tcaatagaga acatgcgtac ttatcaacaa
    15781 agagtgaata acttgtggat aaaaggtgtt gataacgtca tttattgtgg atatcaggtg
    15841 ggaaaacaga aaataaatga ttgattagtg ttaatttact tgtgggtaac taggataagt
    15901 ctgtggatag ctataatatc agcgttttta ttgtgaataa aaaaattaaa atttgtaaaa
    15961 aaataactct aactatagcg attttacagt tagagttatg tatattttta tagtttttgt
    16021 tgtaattctt cccatttaga catagcgtgt tctaattttt gttcgctatc ttgtttttgg
    16081 atagctaatt cgtttgcttt ttgtggattg ttatacactt ctggttgggt taattgctcg
    16141 tctatttgta agatagtagt ctctaaagtt tctatttcat tttcacactc ggatatttgt
    16201 cgttcaagtt tgcgttgttc acgtctttgt tctttttggt tatgatacga cgattgttcc
    16261 atagactttt tatgtgcttg tacgttaact gactgttctt cttggtgcgc tttcaacgct
    16321 tccgcttcct caactttttc aatgtaatat tgataatctc ctaaatacat ctttccgcca
    16381 tcaattgtta aatcaaatac cttgtttgct aattggttaa taaagtaacg atcatgggaa
    16441 acaaatagta ttgttccttc aaaatgttgg agtgcttgct ctaacatttc ttttgaatct
    16501 atatcgaggt ggttggtagg ttcatctaaa ataagtacat tgtcacgttg caacataagt
    16561 agtgctagta gtaaacgtgc tttttcacca ccagataaat cattaattat ctttttaaca
    16621 tcgtcttgta caaataagaa acgtccaaga actgctcgaa tatctttttc attcattaac
    16681 ggatattgat cccacacata atctaaaatc gttttactag atttaaattc tgcttgcttt
    16741 tgatcataat aaccaatttg taaatttgcg ccgaaagtaa tatcgccatt aagcgctttt
    16801 tgttgattag caatagtttt aattaaggtc gattttccaa taccatttgg cccaatgatt
    16861 gctatatgat cgcctttaga gacctctata ctcataggtt tggtaattgc agtttgataa
    16921 ccgatttcta aattttttac atgcatgacg tcattacctg tattccggtc aaagccaaat
    16981 tgaatatttg cacttttggc atctaacatt ggtttatcaa tgcgttccat tttttctaaa
    17041 atcttacgtc tactttttgc cattccactt gttgaagcac gggtaatatt tttctcaaca
    17101 aaagtttcta atcgttttat ttctgcttgt tgactttcat attcttgcat tcgtttttga
    17161 taatataaat cccgttgctg tataaattcc tcgtaattac caacatagcg tttgacatca
    17221 cctaaagcca catcataaat ttgagtaact attttatcta aaaagtaacg atcatggctg
    17281 atgatgacga ttgcaccttt aaaataacgt agataatctt caagccactt tgtcgtttct
    17341 aaatctaagt ggttagtagg ttcatctaaa agtaataaat caggttcgtt taatagcatt
    17401 tgagctaaag ataaacgtgt tttttggcca ccgctaaaat cattgatagg tttattgaaa
    17461 tcttcttcac taaaatttaa cccgtaaagt actgttttaa ttttactttc atattgatat
    17521 ccttctaatt gttcaaattg attcgataaa gattcataac gggacatatg tgttttatat
    17581 gtatcactat cgtaatcatt tgcatgtttt gataaacaat ctgtttcttc tttgattaag
    17641 ctttccattc gtttaatatg ttcaaatggt tttgacattt cttcaaaaac tgttgcatta
    17701 gaatttaaag tcatttgttg agttaaataa cctagtttta agtttttaat ttttgacaca
    17761 tttccaaaat catagttttc tacgccagct ataattttca tcaatgttga tttaccggcg
    17821 ccatttcttc ctacgatacc aattcgttca ccagttttta cttcaaaatc aacatcagta
    17881 aatatatctt caccatcgaa cgattttgat atatgattga gttgtaaaag tatcattcga
    17941 tcttcacctt tcgtctttct caaattttat taacttatga aaaaagtcgt aattattaca
    18001 ttaataacca ttttacagta taaaacgccg tcttgaaact aagatattta tttttaaaat
    18061 tcaataatgt ataatattta ggtattcatt gtaatcaatt atttgttttt taataatcta
    18121 acaattacgc tctaactcgt ttgaatacat agatataatt cattcaaaca cgaaggagga
    18181 aatagcgaat gagtgaccaa gttaaaattc ctcgagcaac tttaaaacgt ttgccgttat
    18241 attatagatt tgtcagttca ttaaaatcta aaggtataga tcgtgtaaat tcaaaagcga
    18301 ttagcgatgc gttacaaatt gactcggcaa caattcgtcg tgacttttca tattttggcg
    18361 aattaggtaa aaaagggtac ggatataata tagatagttt attggatttc tttaaatctg
    18421 aactaagcga gagtgacatg atcaaaatcg caattgtcgg agttgggaac ctagggaaag
    18481 ctttgctcac atataacttt tcaatacatg acgatatgac gattacagaa gcgtttgacg
    18541 taaaagaaga tgttattggc cagaaaatag ggaacgttat tgttaaagat aacgatgaat
    18601 taataacaac attgaagaag gaagaaatag atgttgtgat tctaactaca ccagaaagag
    18661 ttgcacagaa agttgcagat gaactcgtcc aagctggtgt gaaaggtatt ttaaacttca
    18721 ctcctggtag aattaatacg ccttcagatg tgcaagtaca tcaaattgac ttaggtatag
    18781 aattacagtc attattattc tttatgaaaa attacagtga ataaatgtga tgttagcttt
    18841 gaatgattaa ccttgttata tctttaatta attatagaac agtctgggac taagtatgtc
    18901 tcaggctttt tctgttatgt ttgaaaatga aaggaaaaac tgttgtgttg ataaatatag
    18961 tataggttaa taaattaaca cgtagagata taatgatgaa tcgtaattaa tcgccttcct
    19021 tatatgaata taatataaat atcaaaaata ttgtgtataa ggttgaaaac aagaaaatca
    19081 tcacctataa tacatactaa acttatcgga aatgagaggt taagattatg tggatgattt
    19141 ttagtggctt aattgtcggg ggactcttag gttttgtcat gcaaagaaca agattttgtt
    19201 taacaggtgg ctttcgagat atgtatgtgc aaaagaataa taagatgttc tatgcattat
    19261 taatcgctat tactattcaa agtataggat tattgatttt gacggcaaca gatattttac
    19321 aaattcctgc acatagtttt ccaatattgg gaacaattat aggttctttt atttttggaa
    19381 ttggaatagt attggctgga ggatgtgcaa caggtacttg gtatcgcgct ggtgaagggc
    19441 taattggtag ttggattgca ttagtattat atgctgttac tgcagcaatc actaaaacag
    19501 ggattttaaa gccagtaatg gataaaatta atcaaccaac gaatgtaaat agtgatatgt
    19561 ctcaaacaac tggcattccg ttttggggat tagtcgttat attaactata atcaccattt
    19621 ttctagttgt aagaacactc aataataaga aagttcgagt tgctgttcca aaactaaagc
    19681 aacgttatac aggtattaga tattaccttt tcgaaaaacg ataccatcca tttattgcag
    19741 caattgtaat tggacttatc gcactcttag cttggccaat gagtgcatca actggaagaa
    19801 atgacggttt aggtataaca acgccttcag caaatttagt acactttttg attacaggtg
    19861 aaactaaatt tattgattgg ggtgtctttt tagttctagg aattttcatt ggttcatata
    19921 ttgcagctag aggatcaaga gaatttaaat ggcgattgcc agacaagatt acaatacgaa
    19981 acagtgccat tggtggcata tgtatgggat ttggtgcgtc agttgctggt ggttgttcta
    20041 tcggtaacgg tttggttgaa acggcaacga tgacttggca aggatggatt gcgctagcat
    20101 cgatgatagt tggtgtatgg acaatgagtc attttatctt tgttcgtcca atgaaaaaag
    20161 tacaccaaca atctgcaaag gttaaacagc aaacgcaaat agtatagaag attattatgc
    20221 aaatgatgtt gatcaaataa aagtgattgg aaaaggagaa ataattatga tacacgaatt
    20281 aggtacagta ggaatggtat gtccatttcc gttaattgaa gcgcaaaaga aaatggcaac
    20341 attgcaatct ggagatgaat taaaaattga ttttgattgc acgcaagcga cggaagccat
    20401 tccaaattgg gctgcagaaa atggttatcc tgtaacaaac tatgaacaaa ttgataatgc
    20461 ttcatggaca attacaattc aaaaagttta acgttatcat tttaacaata aaatagatat
    20521 tagattctat ggctacttcc gctaatttaa aagtgagtaa gtagtctttt ttgttttagt
    20581 tcatgaaatc atttttatat agtgtggcac attttattcc aaaagatgta ataaaactta
    20641 acgcattttt gctttttata aattgtcaga ttattatgaa aaaaagggag tggtaagtat
    20701 gaatcttaac gatacgatat ttatgttttt gtgtacatta ttagtttggt taatgacacc
    20761 aggattaagt ttattttatg gtgggttagt tcaatctaaa aatgcgctta atactgtcat
    20821 gcaaagtatg gcagcaattg tgcttgttac atttgtatgg ataacagttg gttttacaat
    20881 tagttttggg aatgggaatt tatggttcgg aaattgggaa tatacttttc ttaatcatgt
    20941 aggttttgcg actcaagaag atattagccc acatattcct ttcgctttgt ttatgttatt
    21001 tcaaatgatg ttttgtacga ttgcaatttc tattttatct ggttcaatcg ctgagaaaat
    21061 gaagtttatt ccttatttat tattcgtagt aatatggact gctcttgtat acagtccagt
    21121 agcacattgg gtttggggcg gcggttggat taacaaactc ggtgtattag atttcgctgg
    21181 aggtacggtt gttcatatta catcaggtgt ttctggttta gtattagcta ttatgattgg
    21241 aaaaggaaac aaacattctg aatcaacacc acataatctt atcattacgt tgattggcgg
    21301 tatattcgtg tggattggtt ggtatggatt taatgtaggt agtgctttta catttgataa
    21361 tattgcgatg cttgcattta caaatactgt catttcagcc agtgcaggtg ctataggttg
    21421 gttaatttta gaatatattt ttaaaaagac gacaagttta cttggacttt tactcggtgc
    21481 attagcagga ttagttgtca ttactcctgc agcaggatat gtaacatatc ttagtgcaac
    21541 aataatggct ttaataggag gtatctgttg ttatattgtc attaattaca tcaaggtaaa
    21601 actaaaatat catgatgcat tagatgcatt tggtattcat ggtgttggtg gtattattgg
    21661 tgctgtttta acagcagttt tccaaagtaa aaaagccaat cctgacattg agaatggctt
    21721 tatttatact ggtgacatac atattatact tgtacaaata ttatgtgtaa cagcagttgt
    21781 aatttttagt atagtcatga cgtttattat tgcgaaagta attaaattaa ttacaccatt
    21841 atctgttacg gaacaagaaa cgaatatagg attagacaag attgttcacg gtgaacatgc
    21901 ttactttgaa ggtgagctaa atagattcaa taaacatatt cgatattaga atatatttac
    21961 atagaatatt cattgtcctg acatttaact aaaggttgat gttgggacat tttgttatac
    22021 aaaagtttta ttttgaaatc tttttatgaa agaagcagaa atattattta aagcggttac
    22081 acatatgcta aaataaggct aagtgtcaca aataatgata ggtgaataag tatgaaaaat
    22141 atatctgata ttgccaaatt ggcaggcgtt tcaaaaagta cagtatctag atttttaaat
    22201 aatggatctg tcagtaaaaa aacaagtgaa aaattaacaa gaattatagc agaacatgac
    22261 tatcaaccga atcaatttgc tcaaagttta agagcgagac aaacacacct tattggtgcc
    22321 attataccta gaatgaattc atatgcagta gatgaaacaa tcaaaggatt ggcaaaacaa
    22381 tgccaaaaat atgaatcaca attaatttta aattacacag gtttaaatat cgaagcagaa
    22441 atacaagcgc ttgaaacatt agcacgcagt aaagtagatg gtattgtttt aatggctaca
    22501 gacataacag agagacatat tgaagtcatt aataaaatga atgtaccaat cgttattgtt
    22561 ggtcaacaac atgaacaact tcatagtatt gtgcatgatg attataaagc aggtcaaatt
    22621 ataggcgaat ggattggtca acagggatat caacaagttg aagtgtttag tgtaagtgaa
    22681 aaagatattg cagttggtat acatagaaaa cgtggtttac ttgaccagtt agctaaatac
    22741 caaattaaac ctaatataca tgaaacaaat tttacttatg tggaagcaca aaaagatgtt
    22801 gcaaatgttt tggaaaatgt ggagcaagta gatgcggttg ttggagcaac tgatacgatt
    22861 gcattagctg cctataaata ttattctgat aaaaaagatg ttatgaaacc acatcaaata
    22921 tatggttttg gtggtgaccc aatgacacaa ttagtgtctc catcgataaa aacaattcat
    22981 tataattatt ttgaagctgg ccaatgcgcg atggaagaga tacaacagat gcttaaaaag
    23041 caagatatgc catatagcgt cacagtagat gttaatattt agacgctgta ttttttaaaa
    23101 taaatgtgga accgatacca tataactata aatggatagg ttaaaagtta aagaacgtag
    23161 gtaaaatttg ctataataga atataaattg ttattagcat aaattataaa aggaggactg
    23221 ggtaaatatt atgaccgaat ggactagaga agaacgttat caacgaatcg aggacgttga
    23281 tactgagtat tttaaaacat taaaacaaca agttgatcaa tcaaaatttc gtcaacaatt
    23341 tcatatacaa ccagaaacag gcttattaaa tgaccccaac ggacttattt tttataaagg
    23401 gaagtattat gtttcacatc aatggttccc attaggcgca gtacatggct taaagtattg
    23461 gtataactac acgagtgatg acttaataaa ctttaaagct gaagggccaa ttttaaatcc
    23521 agatactaaa tatgacagcc atggtgtata tagcggtagc gcttttgaat ataacgggca
    23581 tttatattat atgtacacag gaaatcatcg agataatcat tggcaacgac atgcgagtca
    23641 gatgatcgca cgattgaaag aagacggttc agttgaaaag tttccaaagc cagtaattag
    23701 ccaacaacca gaaggatata cgagtcattt tagagatcct aaagttttta aatatgatga
    23761 gaaatattat gcaatcattg gtgcacaaaa taatgatcag caaggtcgat tattacttta
    23821 taatactgaa gatataatta attggcatta tttaggtgaa ataaatacag agttggatga
    23881 ttttggatat atgtgggaat gcccagatta ctttaatgta gataatcaag atgtcatact
    23941 tatttgtcca caaggtattg aaccaaaagg cgatcagttc aaaaatattt atcaaagtgg
    24001 ttatatactt ggaaagtttg atattgaaaa gttaacatat gaacatgaaa attttgtcga
    24061 gcttgataat ggttttgatt tctatgcacc tcaaacattt ttagatgaaa aaggccgacg
    24121 agtactaatt ggatggatgg ggttaccgga aatcgaatat cctactgata atgaaggatg
    24181 ggcccattgc ttaacaattc ctcgtgtatt aaatgtagaa aatggtcaac ttaagcaacg
    24241 tccatatcca gcgttggaaa aattacgtca caataaagag acagcattag gctacgcaaa
    24301 taaatttact cgaaaattac atccgtatga aggtaaacag tatgaattaa tcatagatat
    24361 tttggataat gatgctaccg aagtgtactt tgaattacgt acatctaaga cttcttcaac
    24421 attaattgct tataacaagc gtgaaaataa aataacatta gatcgcagcg acagtggttt
    24481 attgccgaca aatgttgaag gtacgacgcg tagtacgata ttagacacgc cattaaagca
    24541 attacaaatt tttgttgata catctagtat agaaattttc tgtaatgatg gagaacgtgt
    24601 tttgacatct cgaattttcc cgacagaaga cgcactaggt attaaaacat caacagaatc
    24661 agggcaagta tatttacaat ttactaaata tgatttaaag gatgaacata aatgagacgt
    24721 ctattttcaa taggagaagc gttaattgat tttattccaa atgttacaaa tgcgaattta
    24781 aaagatgttc aaacatttac gaagcaaatt ggtggcgcgc catgcaatgt agcttgtaca
    24841 gttcaaaagt taggacaaca agcgtatatg attacacaat taggtaatga tgcatttgga
    24901 gatagcatta ttgaaacgat atcaagtatt ggtgtagatg tatcaaaagt gtatcgcacg
    24961 aatgaagcaa acactgcgtt agcttttgtg agtttgactg aagcgggcga gcgagatttt
    25021 tctttttatc gtaagccctc tgctgatatg ttatttgaac caagttttgt gaatgatatt
    25081 gatgtgaatg aaaatgatgt agtacatttt tgttctgttg atttggtcga tagtccgatg
    25141 agagatgcac attatcaatt gattaccaaa acattaaatg caaatggtac tgtcgtattt
    25201 gatcctaatg taagattgcc attatgggat aacgctgaag atttaagaca aactattcat
    25261 acctttttgc cattggcgca tattgtaaaa gtttcagatg aagaattaga atttattaca
    25321 ggtattcatg atgaaaatga agccattcaa tcattattta caggtaatgt tactgtagtc
    25381 atttatacga agggagcaga tggtgctgcc gtatatttga aaaatggtat taatcattat
    25441 catagtggct ataaggtaaa acctgttgat acaactgggg caggggatgc atttattggt
    25501 gcagttatta gcaggatttt agcaaccgat gtgttaaatt taactcagtt atttgaaaat
    25561 gaaggtgagg aaattttagc gtttagcaat cgcgttgctg caatagtgac aacgaagtat
    25621 ggtgctatta atagtttgcc aacattacaa gaggttgaac aagcatttta aataattcat
    25681 ttataaaaca gtgtaggaaa caagttgaat cttgtgctac actgtttttt aaaacaaaat
    25741 aagattcacg gagtaggaaa ttatagcatt ttatttatgg aaactgtcaa tttcaaattt
    25801 agtatatttt gttaaaaatt gcgccatagg attgtagagt gaaatagaaa ttgataaatt
    25861 atgtagtaat aggaataggt atacaaagag acgttacatc attttttaat tcacgcgtca
    25921 tatttaattt tgttaataaa attaaaaagc cagctataca gtgcatttgc tagttatctt
    25981 gttaaaatcc aacaagattt acaattgaat acgccgttaa ctgactttat tatcttatta
    26041 tattttttta acgtttctca ccgatgcata gcagtgttct ttatttttaa aatagacaat
    26101 tcgctctttc gaatctatag attcaatatt atggcgattg acgacaaagc tattatgaca
    26161 tctaaagaaa cgatcatcta attgactcag ttcttttaaa ttaccataaa attcaatttg
    26221 acggttatct aaatgggcaa tgagtctgtg agattttgtt gatgattcaa aaaacataat
    26281 atcatcatat tgaacataca ctgaattact gccacgtttt aattcaatcg tttcaacgct
    26341 attatcttta gacaacaatt gtaagcgtgt atgtgcagtt tctaaacagt ctataattcg
    26401 agttcttaat tcagctggat catctttaaa aataaaatcc atcgctgcaa ctttgtagac
    26461 aaatgttaaa taggtaagtt cactgtgact cgtaacgaaa ataatgttac caactgggtc
    26521 atgcttacga atttcactgc ctaatttgat accattaata tcagttgaaa gttgaatatc
    26581 taaaaagtaa cagcctatgt cattcatatt tttagcttgc tcaagcacct cataaggatt
    26641 atcagttgcg agggcaattt ccataggctt ttcttctatc attatataat ttttaataat
    26701 ggtaaccatg ttttctcttt gttttggatc gtcttcgcaa atgaaaattt tcatacattc
    26761 acatccttat ggctagttgt taataatttc aactttttga ataaagaaac cattttcgat
    26821 aattgtatct aataagacat tgtctgcatt atcagcaatt tcttttaaag ttgatagacc
    26881 taaaccacga ccttcacctt tagtagaaaa actttcttgg aacaattcat gaatgcgtgg
    26941 tatatcatca gcgcatttat tcataacaat aaacgttact gaattttcac tttcaataaa
    27001 tgcaacgcga atgatagggt catcaatttc agttgatgcc tcaattgcat tatcaagaat
    27061 aataccaata ctgcgactta aatcgatcat attcaagtta atgctactta cttcatcggg
    27121 tatttcgata ctaatcggaa aattcatttc ttgtgcacgt aaaattttcg cagtaattaa
    27181 gcctttaatt tcacgtactt taagattctc gataccattt aattttatag cattcatttg
    27241 taaattgtct ttcataggta caatattttt attgaaataa tcacgtaggc caggcatgtc
    27301 atcttctcga atgtattctg aaagtgtcgt taagatattg acataatcat gacggaactt
    27361 gcgcatttcg ttgttgatag cttcaatctt caatgtatat tcataatagg tttcaatttc
    27421 ttcttgatta cgtttatatt tcatctcttt aaggagaaat tgagaaataa caaatgttaa
    27481 tatacttaaa aatatagtga taccaataaa aataaaagaa tactgcctta ttactttagc
    27541 ttcatccgag tttatttgtg aataaaagaa aaataatgaa aaagtaagca gtaagatagt
    27601 cgaaataact attaaaaatc ctttgtttag tattagatat ggtgtgctaa tttttttgag
    27661 aactctattt attatatatg agaatagtat actaatagtc acataaacta caaaaaagct
    27721 agggaatatt acaaatatac tatcagaa
//
LOCUS       DANRDI010000019        26111 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.019, whole genome shotgun sequence.
ACCESSION   DANRDI010000019 DANRDI010000000
VERSION     DANRDI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.019"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(208..582)
                     /locus_tag="PZ377_000627"
     CDS             complement(208..582)
                     /locus_tag="PZ377_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDM6357517.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(1530..2078)
                     /locus_tag="PZ377_000628"
                     /pseudo
     CDS             complement(1530..2078)
                     /locus_tag="PZ377_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(2298..2834)
                     /locus_tag="PZ377_000629"
     CDS             complement(2298..2834)
                     /locus_tag="PZ377_000629"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDM6357518.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            complement(2815..3318)
                     /locus_tag="PZ377_000630"
     CDS             complement(2815..3318)
                     /locus_tag="PZ377_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDM6357519.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            complement(3427..3783)
                     /locus_tag="PZ377_000631"
     CDS             complement(3427..3783)
                     /locus_tag="PZ377_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HDM6357520.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            complement(3839..4429)
                     /locus_tag="PZ377_000632"
     CDS             complement(3839..4429)
                     /locus_tag="PZ377_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357521.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            complement(4436..5482)
                     /locus_tag="PZ377_000633"
     CDS             complement(4436..5482)
                     /locus_tag="PZ377_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDM6357522.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            complement(5472..7319)
                     /locus_tag="PZ377_000634"
     CDS             complement(5472..7319)
                     /locus_tag="PZ377_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357523.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            complement(7324..8682)
                     /locus_tag="PZ377_000635"
     CDS             complement(7324..8682)
                     /locus_tag="PZ377_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDM6357524.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            complement(8736..11231)
                     /locus_tag="PZ377_000636"
     CDS             complement(8736..11231)
                     /locus_tag="PZ377_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDM6357525.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            complement(11266..11649)
                     /locus_tag="PZ377_000637"
     CDS             complement(11266..11649)
                     /locus_tag="PZ377_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDM6357526.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            complement(11661..11921)
                     /locus_tag="PZ377_000638"
     CDS             complement(11661..11921)
                     /locus_tag="PZ377_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357527.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            complement(11926..12981)
                     /locus_tag="PZ377_000639"
     CDS             complement(11926..12981)
                     /locus_tag="PZ377_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDM6357528.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            complement(13042..14133)
                     /locus_tag="PZ377_000640"
     CDS             complement(13042..14133)
                     /locus_tag="PZ377_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDM6357529.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            complement(14308..14610)
                     /locus_tag="PZ377_000641"
     CDS             complement(14308..14610)
                     /locus_tag="PZ377_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357530.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            complement(14624..14944)
                     /locus_tag="PZ377_000642"
     CDS             complement(14624..14944)
                     /locus_tag="PZ377_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HDM6357531.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            complement(15095..15379)
                     /locus_tag="PZ377_000643"
     CDS             complement(15095..15379)
                     /locus_tag="PZ377_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357532.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            complement(15388..15816)
                     /locus_tag="PZ377_000644"
                     /pseudo
     CDS             complement(15388..15816)
                     /locus_tag="PZ377_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(16011..16235)
                     /locus_tag="PZ377_000645"
     CDS             complement(16011..16235)
                     /locus_tag="PZ377_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDM6357533.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(16252..16455)
                     /locus_tag="PZ377_000646"
     CDS             complement(16252..16455)
                     /locus_tag="PZ377_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDM6357534.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            16614..17174
                     /locus_tag="PZ377_000647"
     CDS             16614..17174
                     /locus_tag="PZ377_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDM6357535.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            17193..19595
                     /gene="mnhA2"
                     /locus_tag="PZ377_000648"
     CDS             17193..19595
                     /gene="mnhA2"
                     /locus_tag="PZ377_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDM6357536.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            19582..20007
                     /gene="mnhB2"
                     /locus_tag="PZ377_000649"
     CDS             19582..20007
                     /gene="mnhB2"
                     /locus_tag="PZ377_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDM6357537.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            20004..20348
                     /gene="mnhC2"
                     /locus_tag="PZ377_000650"
     CDS             20004..20348
                     /gene="mnhC2"
                     /locus_tag="PZ377_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDM6357538.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            20338..21834
                     /gene="mnhD2"
                     /locus_tag="PZ377_000651"
     CDS             20338..21834
                     /gene="mnhD2"
                     /locus_tag="PZ377_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDM6357539.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            21835..22317
                     /gene="mnhE2"
                     /locus_tag="PZ377_000652"
     CDS             21835..22317
                     /gene="mnhE2"
                     /locus_tag="PZ377_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDM6357540.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            22314..22616
                     /gene="mnhF2"
                     /locus_tag="PZ377_000653"
     CDS             22314..22616
                     /gene="mnhF2"
                     /locus_tag="PZ377_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDM6357541.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            22591..23028
                     /gene="mnhG2"
                     /locus_tag="PZ377_000654"
     CDS             22591..23028
                     /gene="mnhG2"
                     /locus_tag="PZ377_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDM6357542.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            23368..25410
                     /locus_tag="PZ377_000655"
     CDS             23368..25410
                     /locus_tag="PZ377_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDM6357543.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
BASE COUNT         8671 a         4299 c         3644 g         9497 t
ORIGIN      
        1 gaaaaagctg attactattc attatttgat agaaatcagc tttttttgaa atgtatttga
       61 tatatactgc tcgttatgcg gctatcttcc ttatattaag tgccattagt gcaaaacctc
      121 ttaacaatta ggtaaaaaga gcataaaaaa aggaagttta atagaatgta tcatctatca
      181 aacttcacca aattgcgcta aacaaaatta tagttcaatt tcgttgtttg cttcagtgat
      241 tcgtttattt actcgactca ataatgattc gattttttta cgttgttgtg cattaacaag
      301 aattaataca gttctttcat catgctcatt acgtttttta tcgaagtaat cttcttgaga
      361 taaaatttta actgctttaa caacttgtgg ttgtttgtag tttaaatgat taataatatc
      421 tttaagatag tattctttct ctttgttttc gctgatgtat gtcaatacag cgaattcttc
      481 aaagctaatt gaaaattcct ttttaattaa actttttaat ttgtcagcat aagtgaccat
      541 tgataacaac tcaaagcaat cattgatttt tgtaattgcc atgtttaaaa cctccctatt
      601 tgatgcatct tgctcgatac atttgcccga taatatattg atatctaatc tttatttatt
      661 atagatatgt tagtcataat tttgcattaa ataagtttta ttaaatatat ttaatgctct
      721 attatttagt taattataac taattaaaaa tgagaagtaa acaaaaaagt gtttataaaa
      781 caaattattc attagacaca gtgattgtat ttctgggtta gcatttggtt tagtcaaaaa
      841 tatcagcatt ttttataatt taccttaatt taatcgacaa tacaaggtaa tttatttaat
      901 gaaacgattt agcgcaatta aatgtttcga tattcaatgt gtgtctaaag agattctttg
      961 ttatagctta actcttccaa gttaacttct tctactattt atacccattt ttcagaattt
     1021 tatcactaaa aatgaaaaat ttttataatt tttttaattt agtaattctc agtgcagttt
     1081 taatgataag aaaaacacat taatttagtg attaaaacca atctatagca cgttaaagtg
     1141 tctatacgtt attccgattg ataacgggta acaaataata gaaagtacga taattgatca
     1201 tgcattaaag cacttttatt tttcaaactc gactcaatga attataaaaa gttgtgcatt
     1261 ttgacttatt tttaattaat tgaataacca actatgaaat attagcacga ataacataat
     1321 ttttgctatt aatttggtgt aaattattaa aaaataacat taatttaggt cttaagaatg
     1381 agttgactat cgaatttgct aaattaaaat ttgttgtgtc aaagcatact acccaaatca
     1441 acgcttttct aaagattttt agaccacaaa aaaatagctt atcactttta aaaataatta
     1501 caagttaaaa tgataagcta aaagttaatt taaaataatc ttaaaagtat aatgcctgtc
     1561 aaaatacata atagaccaat agtttttctg gatgtcattg ctattttagg tgaaccaaat
     1621 aatccaaagt gatctatcaa tatgcccatt agaatctggc caaacatccc aataagtgtt
     1681 gttaatgctg cacccatatg aggcattaag ataatgttag ctgttacaaa agccatgcca
     1741 agtataccgc cagtaaaata gataggcttt aatttaccga attttaaatg acttgttttt
     1801 agttttaaag aacgattaaa aatagcggtt aaaatcaata gcgctattga cccaattgta
     1861 aatgatacta atgatgcaaa ggctggtgaa tgagtatgac tagctaaagc actattaatt
     1921 gtcgtttgaa taggtggaaa gaaaccaaaa ataaatccta atagaagcca aaacagtaaa
     1981 tacttttgat cagttagtaa taaattattc ttgttaaatt gattcattat gacgatgccg
     2041 acaatgagta acaatactcc aattgcttta attaaatggc agtgtgtcaa tagaaaaatg
     2101 agaatttctt aaattttttt aataaatatt actaaaagag tacagaagaa gaaaaagaag
     2161 cattttacta gtaatatctg tcaaaaacca cagatttaaa tagtatgctt ctttttagat
     2221 tatgcatttt acatacaaaa tttattcgtc ctgtccttcc gcgctcattg taaaccattc
     2281 gcttagatta acctatatta attgacgggt tcgtgcctca cacagtcatt taatataggt
     2341 tcacaatttt gtgtgcttac atggtacttt agcatttcct cactcgattt atagtcaaat
     2401 atcattcgag gccgtgcatt aatttgttta acagcttcat ctaatgactt ttgtgttata
     2461 tttgcaaaat ccgtaccttt aggataatac tgtcttaaaa gtccatttgc attttcaata
     2521 gaaccacgtt gatatggaca acccaaatca ctaaaataaa tactacaatt caattcatct
     2581 tctatttctt caaatagagc aaactcctta cctctatcca tagttatact tttgggtaat
     2641 aatggtttca taatatttat aatctcatta caaactacat tggatttacg tgtatttaat
     2701 ctactagcat ataaatatct tgtctttctt tcaataatag ttaacaagca atatgttgat
     2761 ggtctaacac tccaaacagt atcaatttca taatgtccaa attcttcacg attattaata
     2821 ctttttgagc cgatacttta taacacgaga ccctacgtct aatttgccac gcttttcaga
     2881 agacttatta tatttcttgc ctgcttgtct catatcacgc ttaacatcaa cttttaccaa
     2941 tccattgcga atatatctat atatagtagg ataacttata taataccctg tatctttttc
     3001 aatttgactt gctatctgtc taggagacca ttttaatgaa agtttttctt caatatatgc
     3061 ttttgtttca tcattcaaac gtctacgttt agcaacttgt tcatctgaaa tactacgcga
     3121 ttttatagca tcttcctcag ctaataaagg attataaaca ccatcaacag aacgcttatt
     3181 tacttctcta taaagaactg catcatcacg acctaaaatc ttagaaatct gaatgatgtt
     3241 taaaccatca tttaaatact ttgcgatagc tagacgtgtt ttatagtcta attctttacg
     3301 tttttttctt tttttcatgt atatcctcct ttacttttaa aaaggttatt tataaaataa
     3361 gttataagag gatatatgtt catcaactcc cgataccatc gctaaagcgt atagggagtt
     3421 gattctttat tttttcatat tattttcttc ataaactggt ttattatctt tctcaaactt
     3481 ttcagcatca atattagaat cttcttcata tttatcgcta gtctgattta ttttatatac
     3541 tcttgaaact tttgtattac tatctttaaa aggcatataa gtcattataa taacctcacc
     3601 atcattataa atcttataat aactttttga ccgctcaaca tttttatact tctttgtatt
     3661 tttcacatct tgaatacttt ctttttctaa ctttgtaact tcatcaattt ctttatcata
     3721 tttctgacca catgcagata ataatataac taatcctaaa attaatacaa accatcgcct
     3781 catgataaat tcactccttt ttacgtaaat tatatcattg ctatcgtctt gaaacaaact
     3841 aacgtcctag aggttttaaa ttgaaacgtt ttaaattatc aatacgccaa ccttcagctg
     3901 tacgaactaa atccaattgt gcagatgttt gacgatgttc aattttatca tctccatctg
     3961 tttcaatacg ttcgtcgaac tgaacaaaaa ctttataact atctttttta gggtcataag
     4021 tatcaaaata aacatgaata tcattaaccg ttatatcatc atataaatca tatttatctt
     4081 tagaatcctt aaataatgta tcgtaataat cctttgtaac taaaggcttt atttgtggtg
     4141 cttttgattt aaaagaagaa tcttttttca tttcaaacat ctgtttaaca aaatttgaag
     4201 aaacttcacg tatttgtttt tcttcatcag aaataactgt ttttgattgt ctctttacta
     4261 atttatttaa ctctccattt ttatctctta atatacgttg ttcttcctgc aaatcttcaa
     4321 tttgattttg tttcatttta acttgttgtt taggaatctg ataaccaatt aaaacagcac
     4381 aaaataatat tactgttaca agagtaaaca taataatttt ttgcttcatt tattttcact
     4441 ccttatcatg aataaaacta tatccgtcag tcattttcaa tgtacgttca ttaactttaa
     4501 aagcaccttc ccaattcatt tctgttattc taaatgtttt accaccatca cttacaccaa
     4561 taacaacagc cacatgacca taatgcccat catgaccgcc tgcaccttgt tcagcaataa
     4621 ataatgcacc aggttccgga tttttatcaa ctttataacc ctcagcttta gctctatcat
     4681 tccatttatg tgcatcatgc caataagtcg atacaggttt acccattttt tgacgcatac
     4741 cgtatgcata ccaagtacat tgaccagcag tgtataaatt accttttcca gggtcatcat
     4801 ttgaccaatg tggcaattta ccattaaact ttatagactt tcctttacca ttactacatg
     4861 acgttttagc ttctgaaccg cctaaatctt tcataatttt ttcaactgtt ggtatccaac
     4921 gggcattcat attattaggg tcatttgacg caccaacagg cgcatatttg ggacctattt
     4981 ttttaggagt atctaaacct ttagaaatat aaacatcata caaattttta gctcccgcat
     5041 taagaccatc ttcaatagtt ggatatgttg aatcatgaat cgattttgaa cccataacag
     5101 acaaaggatt attttgtctt gtcgcatttt ctcctttacc ccattgtgat tcactcgcaa
     5161 taatggccat aaaaatattc acaggtactt tatttttttc tgcaattttc tgtatttctt
     5221 ttcctttacc ctctaatttt ccacctttag cattttcgtt aaacttatca ataccatttg
     5281 ccgaaacatt accgccacta acagtacaac cagcatcatc tttaccttta tttaaatcag
     5341 gcgctaacaa aaacattata attactggaa atataaaaaa agccaccaca cataaaatga
     5401 tgatgacttt cgctaatttc aatttaacgc ctattggcgt tttacctatt acttttttag
     5461 caatatcttt tttatttacc atgcttattg atatcctttt gctcttttaa acggtctttt
     5521 gactgagaat tacgcgtaat aggttgatta cgatttccag attgtctact ttgttgattc
     5581 tgtgtcgcat tttgtatttg tcgtttttga acatcagtat cataagcagt ttgaactgac
     5641 ttctgttctt tacgatttat tgaatgattc tcattattta atggctgatt aaccacttgt
     5701 ggcgaccttt gtatatcttt tcgctgtctt gtttctcgta cttgatgttc ttgctgtgat
     5761 gctttttgtg gttgtttaga ataattattt gcatttgatt caacagaagc agattttaca
     5821 tcgttaccat tcattgactc ttgttgttct ctgcgcttat taccattttt ttgattatct
     5881 ttatttataa ataaaggcat tggtttatcc tgttgtcttc ttcttaaatc attatcattt
     5941 tgaagtggtg gggcgggttc atctctttga ttttctcgtt tagctttctt attttgcgct
     6001 tgttcatgac gaatcttatt aaaacgttct tgtgttcgat tcatcacacc agcaccaatt
     6061 gcactaggag aaaaactaat attaccacga cttacaaaat tgataatttg agcccgtttt
     6121 ttatacatta caaacaatat tacaattata aatattgaat ttaaaaaata agcattttga
     6181 ctatccggtt caataaaact atttgcaagc ttgatacaca aaccaactat cacaaataag
     6241 aatgtcatac aagcacttaa aataatatag cctaacgttt ttgcaaacgc agagaaaaca
     6301 gataattcaa atcctggtat aaaactcact atccaactga atactaaaaa taagaataaa
     6361 aataaagcaa atgtagaaaa aatcaacttc gcaaaactta ttgataaaaa tactatcgac
     6421 ataaaaagtg taataataaa accaatcact gaaatagcca acttttgaaa tacataacct
     6481 tgcgtcatat atttattatt tttcttttca acctcatctt taacaatatc atctaattct
     6541 ttttctgatt ttttatcata atcaagtttt aaaatgttat caatacggtc tttacctttt
     6601 gcttttatct ctttttcgtc gacagttcca taattcatta ctaaatatgt tctatatatc
     6661 gtcatatcaa ataattgatt acgtatatga ttcatacctg aattctcatg aatataatca
     6721 atattaccat caatagaaga tgtactattc ataattatat tatttaagtc cttaccaata
     6781 ttgtttgtac cacgtataat attacctgca ttcgcaagta taccaacacc aaaaccaatt
     6841 aaacacataa ttaaaaacgc tcgttttaat gcttctcttg cattaccttg tacactaaaa
     6901 ataataaatg cgttaaaaca aacaataata aataatgtta atgctaaatt actcaataac
     6961 ttttgatata tatcgccaac aaaatcgccg attttatccg caaaatcatt aataacatct
     7021 aattgaaata aattttcaat cgcataatct gtaacacctg ctaaaatctt agtcatagaa
     7081 aagaaaaaat ctgacacatt attaatttga cgtgaaattt cttcttgacc aaacggattc
     7141 cagtcgcctt tttcactata tattgaacga taatgtgata aatcatactt ttcaattttt
     7201 ggcttaactg catgtgaggg ctctccaact ggattagaaa cagcataagc actcacagat
     7261 gttaaaagca gaataggcaa aattaaaaag cataaaagat acacactttt atgcttcatc
     7321 aaatcactcc ttatttaata tttgtttcac atcttccaca aaatcatatt gttccattaa
     7381 aggtgcatca aataaaattg gttcattata ttgtccaagt atagaaatat aacctcggcc
     7441 gatatcacta tcatgaatcg tttgaaattc tttatcagtt tgaccaaagg tcatacgata
     7501 accatcattt gacaaattac ctaaagacac acgtaaccct aattggtcac gcacattacc
     7561 tttaatcaca tctgcatctg gtctttgtgc tgttaaaaac ataaatacac cagcttgacg
     7621 acctttcata ataatattaa ttaaataatc attcatttct tgtaattctt ttttatctaa
     7681 agttgaagta aaagctgtta cctcatcaaa aataatgaaa taggggcgca tatcaaaatt
     7741 acggaaatca ttcccgattt tataatccga actatcattc attaatctaa aacgctcttc
     7801 catttcatta ttagcttctc tcaattgttt taatatttga cctttagtat ctgcaacttt
     7861 atcatcacca ataacgcgct tcataaagct caaatctgaa acttttgggt ctaataaacg
     7921 tacttctgaa tgtctacgga ataactcacg aatcacataa aataaaaaat atgttttacc
     7981 acctccggtt atacctgtaa ccaatccatg aggtgctttc acaaaatccc aagtaatttg
     8041 atgcgtaata gcaatttttg tatcataaaa aattgtattt gattcttgtt cgccattcat
     8101 aacaatacgc tttttatatt taagtggcat aaattcatat ctcaaataac gaccaatatg
     8161 ctttttttct actaattcag aattaaacat tggttcaata atcgtctcta actctaaata
     8221 attatcttga aacctcgaac cgtctttctt aatatgaata acaaaagatt ttttaccacg
     8281 cttataaaac attgttggat aataatcata ttcagtttgt ttataatcaa tagggatata
     8341 ctcagttgtt ttcttgatta ctgtacgatt atgaaaacct tgtgtttcaa tcatttcagc
     8401 caaatctttt gttttaaaac gtattaaaaa ctgagcaata agcttacata tcacaaatat
     8461 aattgctgta ccaattaata aatacaataa atgcggttta aaatatgcaa acaacgtcaa
     8521 tatatccaat tttttattaa ctgatttata atcaataaac aaaatatata tacgatatac
     8581 caaaaaaata aatgctaata cacttgcaaa cataaaatca atatacttct gataaggctg
     8641 tatacgtacg cctttaaata aagcaatttc attcattgcc atcaaatcca ctcctttaaa
     8701 ataaaaagca catacaattt tgtatgtgct aaaaattaca tcgcaaattt ttcttcagct
     8761 ttagagcttg cagaatcttc ttgtgttcta aatgcttcag tcatttcctc aaatggacaa
     8821 tgtacaacca tttttccaac acgtccataa aaatcactga atatacattg acctttcttc
     8881 aaatctttca acatctccat gttttcagga gattcttttg aatattctaa gcctaaagat
     8941 tttaaaatat cctcacgttc atctttttca tcaaatgcaa aatgacaacc aaagttacca
     9001 ccatcatcat ctgctttatc ttttaccgat tgtgtaataa atacaagtga attattctca
     9061 gaacgtcccg ttctttttat acgcttacca acttttttac cttgtcttga agcactaaaa
     9121 gcccaaccct catcaataaa tatagtcgtt tgaacatctc tacgagaacc gaatttctct
     9181 aaaaacttac caattaataa cattaatgta atcccatttt tctcacttgt tgaataactt
     9241 gatgtatcat catctgcttt aggcatatct aaacctttaa cctgtaaaat agttgctttt
     9301 tcttgaatat ttaaagctgg attcgtacca tcactaaaaa caagttttaa aatattgttt
     9361 tgcgtttttt caaataataa atcgcctgca ttttttacat gctcatcttc attattctgt
     9421 aatttttcaa ttacatttaa tgaaccaact tttttaccat tttcacgttc ttcaaccgta
     9481 tcagaaatag cttgataaat tgctgtttca atatgatgat aattttcaaa agatttaatt
     9541 tcatcaaaca ctgatttcaa catctcatta gcttcaatac catctaaaaa tacaataggg
     9601 tctagacaac cataattggt tgaatcttca taattcaatg ttacatattt aaatgttttt
     9661 aaatgattaa taaatagggg atacttttct tgcatttcct tactttctgt tactctgtca
     9721 aaccattctg caaactcaga ttttggatcc cagtacaaca tttgaccttt aaaaaatgat
     9781 gtataaatcc ataataattt tgctaaaaat gatttacctt tcccagtttg accagtaatt
     9841 aaaacatgcg gactatcact gacagccccc ttaatacctt ttgcggccaa caataaacta
     9901 aacaaaatta tatcccttga acttgcaaca gcttcctcac gtgaaacaga acgtataaat
     9961 ttatcaatac gccctatata aaaacccgtt tttgttccca aactttgaga cacaccaaat
    10021 aaattctctg ctatacctgt tgccgttgtt ctttgtaacc aatacttatt aataaataaa
    10081 tcattaccat gtaaacattg ataaaacaat tgtaactggt cagctaatgg attaactgca
    10141 tatatttgag tttcctttaa tcgtttcatc aaacgctttg ctttacgttt acaatctcta
    10201 taatcatcac ttttaattac aaacataaat acccactcaa taaatacacc tgagccagtc
    10261 acttctcgtt ccatttgatt caatacaaac aaattatctt gaacatcttc cgaagcactg
    10321 tcgccagtat cttgcttttc ttttgcttgt tgtttatgat ttattttcgc acggtcaaat
    10381 tttttcttaa ctttctcatt attaatgatt tttgctttta catggcattc aaccggaaaa
    10441 ggcatatttt gagctttctc aaataaatgt gtatattcca tatctaacgg aaaatcatca
    10501 acaacaactg ttgttgtata aaatttctcg ttatcatttt caacttctaa aacactcaag
    10561 cgagtagggt caattactgc attataaata tctgtaatag ctcttcttga agattcttcc
    10621 tgtacgtcat gtgttgcatt tgaaataaaa ggctttctaa taatataagc caattcattc
    10681 tcagttaaac gctcgcctcc aatacctaga aaactatcag ccaaatctcg ttcaaatgtt
    10741 ttatacttat ctaaaatttt atcgtctaaa ttcacatcaa aaccaaaatt cgaaacaata
    10801 cgctcagccg tatctgataa tgtacttttt aaaatctctt taagtgtaac tgcatcgtca
    10861 gctaattcac gtattttaac acccataaca aaagaattat gcgtaatcat ttgtaattct
    10921 tgttctaata cgtcaatagt ttcttttgaa taatattcag ctacattaaa agcttcatca
    10981 tcaaaatctt tactaatttc tttaaattta tttcgcaaat ctaaatcgtg gtcatacatt
    11041 tcaaaatgaa aatcttgata acctttaatc gactctaaaa aataacgcat acgctttttt
    11101 gattcttctt ttgcatcata atttgctgtt gatatagact cagaacgaac acgataatac
    11161 gcccaaactt cgccagtttt cgttaataac aaattatctc ttaaacctgc atacggtgct
    11221 tttaaattca tgtcatccct ccaataaaaa taaccccaat aggggctatt taaatgttat
    11281 ttttttatca tgatattcta cttcttcatc ataacttaat ttaacttttc ttaactgcac
    11341 attaaaaaca aatattacaa aatcccataa gaagaaaaat aatttctttc catcttgttt
    11401 taacttaaca atatatttcg ttatataata aggtgcaacc gtaaagtaaa taaacataag
    11461 tccaccaatc gacataatta caggctgtaa taaataccaa aataacaaaa ataatataaa
    11521 ccacacaatc aaaaatgtag cccaaaaatc taaacgaaaa cccttattaa tacgaaactt
    11581 atctgtaaat tcatacataa ctatcggttg ttcaaaagtt cgctttaaat tataagcttt
    11641 tttatccaaa tggaatcacc tcaaccgcca aaaatcattt tccaaacacc tgcaatcgca
    11701 ttaaatacct gttcaggacc tttagaaact aagaataaaa agccaccaac aacaaataat
    11761 ataaccatat gcccatattt accactagcc cattttacaa taccaacaac caatactact
    11821 aaaccaatac ccgtacctac ttcattactt atccagtcac caactccacc aagtgatgga
    11881 cggtcagcac ctaaaacaaa aaaattaaat aaaccactca taacattaac ctccaataga
    11941 attagtcata tttttaacgt aatatttacc atcttttttt gttatatcta atgtgtaatg
    12001 ctccaaattc tccaaaggag aatctttatc tttcatcaaa atttcaactt tagcaacata
    12061 atcatcgcct tttggatata acctaatttc tcttatctga gaaacttctc tagttccttc
    12121 taagccctca gggttatcca ttaaataggc catatcatca gacttactag aagcatattt
    12181 attaaagaaa tcttcaataa aagcttttgc tttcggattg tcttcccgtt tattgtcttt
    12241 caaattatcc tcaaccattt tagcttgatt caattgacta tcagggattg gcgtaaaata
    12301 aggatattca acaacaacat acttattatt ttcactctta ataggtatat ttattaaaat
    12361 gttttgattt actttacgtt gcttttcttc tgtttttgtt tcatactcat ctttatcttt
    12421 ttcacttttc tttttcttct taactttaac ttctttcttt tcagtaatat tcacatcata
    12481 attaacaata tactgtataa ttgtttgttt atctttacgt ttaatattat aaaattcttt
    12541 actattcaat tttcgttctg tatctgaaat tttttcatca ggttttttat aatctgatgg
    12601 aaaatatttc aacaattctt tttctctaga ttccaattct tttgaatctt tcgagatatt
    12661 catatacgta tcaataaact tatcagcata taactttaat ttaggactat actgaactgt
    12721 attcgcatta tcttcatact ttttttgaat catattcgtt ttattaactg cttctttact
    12781 gtcattattt gctcttgtag ctttaataaa agaagctaat aaaagaatta acaaaatagc
    12841 taataatgag taaaaacaaa ttacaatcat cttacgacga tttttaaatt ttggaattac
    12901 tcggcgttgt tctttaggaa tatcaaaatg ctttccgttt ttagaaaatc gtgttaaaat
    12961 gtacttgctt attattttca taaacgattt tctaaaatcc tttacgaaat ttttaataaa
    13021 aatcacatcc cagttatttt tttaacactt atcttttgat aagtgctttt tttgttttaa
    13081 agcctccaaa taatgattaa ttctattcgt atgctcatca ttaaattgtg aatgcgctaa
    13141 aattttatct aaaaaatcag tatctgtaag caacccctca gattgcgcca tactttcggc
    13201 agttaacact gtatctaaag tagtagcaac ttgtttacat aaccaatcaa gattatcctc
    13261 aatggacttt aatgttggtc tagtagttaa attcaatttt tcagtatctt taataaataa
    13321 cgcccaaggt ctatataatg gccaccgctt acgattttta tcatttccac ctttaggagg
    13381 agagataaaa cgcaaattat tatgcaatat agactttaca atttcgctta tattatctgt
    13441 tctactcaac actttcgcac aattaactgc atatgccttt cgcatttgta tttcataacg
    13501 attccatagc ccaatgtctt cgacatcaca atgacgttta aacgcttgtt cataattctt
    13561 ttcataaaaa cgacaatata aattgctttg tttagaacca atataaaaag tagcgccttt
    13621 acttcttccg tctgacaaat taaaaccgtt tattgcgctt ccagctctaa aacgtgaaac
    13681 acactcagct ttttcagcct ttttaattaa taaaggcact tttaaatatg ttttataatc
    13741 atcaattgca atatcaattc ttgtaaaatt tgcattaact gataaacatt tgtcaaaaaa
    13801 atcttgccaa gttttattca actgctgtaa atgatgttca aactctctac aacctgtacc
    13861 agttaactgt aagtgaatgc ccattgattc atcatcactt gaataataca atttaatgtt
    13921 tgaaaattca taaccgcgat tataaaagta aatgccacta tttcgttttt tgaacaacgt
    13981 tataggcaaa ccgataacgt cctcaattac tgctgaaata ggggctatat ggaaagtgac
    14041 ctgaacccag tcaacaactg cctccacagc acttttattt gtacgctcta cccccctatt
    14101 agtaaggggg gtacactggt tttttaaagt catatttatt tatgcctcct atcaaatcgc
    14161 aagcgatttg atacatgacc gttctcgctt ttttaacgcg tatttgtcgc aggctccgcc
    14221 caaatgctta acgcactccg tgcttgcatt tctgcgcctg catacgctaa aagctacacg
    14281 gtcatgttaa atcactaata ctatatgtta aacatcaata tcgccaatat aaatacaatc
    14341 ttccaaattc acttgtagca ttctgccatg tcctgcttca ctcaagctca tacacaatag
    14401 acaaaggtca gcgtaaattt taggccgacc ttttaccgta ttttctttaa cgtaactatc
    14461 attaaattta gactgtattt ctttagctaa agtcattaat gaaactttat cgccccaatt
    14521 tatacgacta gcaacatagt ttaaaatagg ttcactcaaa tttgaactat ttgtgtagcg
    14581 atataatcta tctgctgtat aataatccat ttacatcacc taattatttt tgtttaagtg
    14641 cactattaga accaactact ttaatatctt ctgcattcca gctatatgcg atagttccaa
    14701 aatcgcctga catttgaaca tacattttaa cgtctggttc aactaattcg cattcttgta
    14761 aatagtctaa ttccacttta ttaggcgttt taacagtgat ttgttcgcct tgttcttgac
    14821 tcgctaattt atatgcataa acagttttat cagtacgctc tccattttca tatttatatt
    14881 tttcatctac acctaaaaag tttaattgtc ctaaagtttt tttaatatcc aatactggtt
    14941 tcataatata tccatccttt acaaaatttt taataaaatt aaacgacttc taaagggtcg
    15001 cacgaatagg aataactccc gcaaatccgt accactgcat ttttgataat ggagtgtcta
    15061 tgcttctcct gacctatcat atttttaatc aaacctatcg gttcatcacc tcaaaaaatt
    15121 ctaagtcttc aattgaaata tgatacatct tacaaataaa tgtattagac acaccatatt
    15181 gttttaaaaa tttataatcc ttataagtaa atcgataatc attctttttt gccatttcac
    15241 aaacttctaa tatacgaaaa tatgcaaaat tatacatttt acaaattttt cttttagaaa
    15301 caccatgaaa tcttaaaatt acaaatttaa gataatttaa ttgatttgaa ccatcaaaaa
    15361 aaggtgtaaa ctcagtcata attatcaatt cctttcataa gaattaactg ttaactaaag
    15421 tttacaccac caattaaaat catgaattgt agcgccaaat aatccaaatg tatcaataat
    15481 gacacccata ataatttgac ccgcaactgt tgcaattaca gttaatgttg cacctaattt
    15541 tggcaataac aataaattgc cagttaaaaa gctaacccca agcaaaccac cgactaccca
    15601 tgtgtagtta aatgattgat tattgtaaaa gtgaatagta aatacttctg gattgataat
    15661 gatatttaaa ataattaaac aaattgttcc aactgaaaat gaaatgaatg aagtatagaa
    15721 aggcgattta gtatacagtg atagtcttga attgacagat gtttggatag gaataagcat
    15781 tccaactagg acaccaatga tatagaaaag aagcatatga ataaagtctc actttccaaa
    15841 gttaataata aaatcatata tttacattat cattatagag aaaatataca actcatttac
    15901 aataatgttg aaaaaaacag gacattatta caacaaaatc accctagaca atcatgatga
    15961 aagtctaggg tgttagtatt tatactaaat taattgcgct ttctttatat ctatttctta
    16021 tcttcaaata aaacagttgt tgttttatca ataatttgag cagaatgtac tttggtgata
    16081 gtaccactat ttgactttat aatatttaaa tctactaatg cattcatact atcacgtgca
    16141 acttgctcgg tcactacgtt gtttaattgt ggtaattgca gttttacagg cttgtctagt
    16201 gaagatttaa aacttaattc aagtgtttta gtcatgaata tgtcctcctg attaaattga
    16261 taaagatttg atgagttcga tattgctata tgtttctcca gtaagtcgct caataagttt
    16321 gctgaatgtt ttaatttggt cgtttgatgc atcagggtta atgttagcga atcgacgctt
    16381 aaattctgtt tgtttgccgt tagcgtctac tttagtaaat gataatacaa tagtgatgtg
    16441 gtttatttta ctcatatttt aaaacctcct ttcacactat atatcgaaac aaaataataa
    16501 aatggctaat tttattttct atgtttaaaa tctataaaaa aggcaataga tatatgtaac
    16561 taaaatataa tatagctatg attaagacac ttaaagcaag gggaggttga gtaatgaata
    16621 aagtagaagc gattaaattt aatgatgata ttgttaaaat gtatgaagcg ctcaagataa
    16681 aatctgaacg tgactattta ttctttaagt tagctataca tagtggattg aaagtatcag
    16741 aattattaac aattacagtc tctcaagtta agagactaat tgaaaagtgt acgttatcag
    16801 aaatgtgtaa agcacatttt cattcgttga ttaaaattag gttaccagaa acattatcga
    16861 aagaactact tcaatatata gaggacagga gtctttcgaa tgaagacgtt ctttttcaat
    16921 cactacgaac aaatcaagta ttatctagac agcaagcata tcgaataatt caccaagcat
    16981 caattgaagc tggtatagat aatgtaggac taacgacatt gcgtaagaca tttgcatatc
    17041 atgcttatca aaaaggtata cctataccag tcattcaaaa gtatttaggg catcaatctg
    17101 ctattgaaac actaaatttt atcggtttag aaaatgagtg tgaacatagt atttatattt
    17161 cattacaatt atagaaacaa aggaggctaa taatgagttt ggtttattta ttaattgcta
    17221 tacttgtgat tatggcgatg atacttctaa tgtctaaacg tagagcattg gctaaatatg
    17281 ccgggtacat agcgttggtt gcacctgtaa tttcatctat ctattttttg attcaaatac
    17341 catcagtagc taaactgcaa tatctttcta cctctattcc atggattaag acattagata
    17401 ttaatttaga tttacgttta gatggtttaa gtttaatgtt ttctcttatt atttcactta
    17461 ttggaattgc agtattcttc tatgcaactc aatatttatc ctctcgaaaa gacaatttac
    17521 caaggtttta tttttattta acgttattta tgttcagtat gattggtatt gtattatcag
    17581 acaatacgat attgatgtac attttttggg aattaacgag tgtatcatca tttttattga
    17641 tttcatattg gtataacaat ggtgacagtc aatttggtgc gattcaatca tttatgatta
    17701 cagtatttgg tggattggcg ttattagttg gttttattat gctgtatatc atgacaggaa
    17761 cgaataacat cacagagata ttaggacaag cagatcatat taagaatcat ggattgttta
    17821 tccctatgat ttttatgttt ttattaggtg catttacaaa atcagcacaa tttccatttc
    17881 atatttggct acctagagca atggctgcac ctacacctgt aagtgcttat ttacattcag
    17941 ccacgatggt aaaagctggt atctttttat tacttcgatt tacaccatta ttaggtctta
    18001 gcaatatgta cgtatatatc gttacgtttg ttggtttaat aacaatgtta tttggttcaa
    18061 ttacagcttt aaaacaatgg gatttaaaag gtatcctagc gtactctaca atcagtcaac
    18121 ttgggatgat tatggctatg gtgggtatag gtggcggata tgctcaacac caacaagacg
    18181 caatagcatc tatttatgta tttgtattat ttggtgcgct atttcatcta atgaatcatg
    18241 ccatctttaa atgtgcgctt ttcatgggag taggtatttt agatcatgaa gcaggttcaa
    18301 gggatatacg aattttaagt ggaatgcgtc aactatttcc taaaatgaat ctagtcatga
    18361 cgatagcggc tctatctatg gctggagtac catttttaaa tggattttta agtaaagaaa
    18421 tgtttttaga tgcattaaca caaactggac aattatccca atttagtttg atttcaatga
    18481 tagctatcgt gtttgttggt gttattgcga gtgtttttac attcacatat gcactataca
    18541 tggtaaaaga agtattttgg acaaaatatg attctaaggt ttttactaaa aaaaatatcc
    18601 acgaaccatg gttgtttagt ttaccatctc ttatattaat ggtgctagta cctgtaatct
    18661 tttttgtacc aaatatattt gggaagggga ttatcgttct agcattaaga gctgtatcag
    18721 gtggtaatca tcaaattgat caattggcac cacatgtttc gcaatggcat ggatttaaca
    18781 taccgcttct tttaaccatc atcattattt tattgggtag tgtactagca atcaaagtag
    18841 attggaaaaa agtgttcaca ggtaaaatta gacagatttc agtttcaaaa agctatgaga
    18901 tggtatatcg acattttgaa aagtttgcta cgaagcgatt taaacgtgtt atgcaagatc
    18961 gtttaaacca atacattatt atgaccttag gcatatttat gattatcatt ggatatggtt
    19021 atattcgaat tggacttcct aaagtacatc agttacatgt ttctgaattt ggggcattag
    19081 aaattatatt agcaatcgta actgtcacaa ttggtatttc tttaattttt atacgtcaac
    19141 gactgacaat ggtcatttta aatggagtca tcggatttgt tgtgacctta ttctttatag
    19201 caatgaaagc ccctgatcta gcattgactc agctagtagt tgaaacaata acgacgatac
    19261 tatttattgt cagtttttca agattaccaa acgtgccaag atctaacgct aacaaaaaaa
    19321 gagaaataat taaaatttct gtatcactct tgatggcact tattgttgta tcattaattt
    19381 ttattacaca acaaacagat ggtttatcat caatatcaga cttttattta aaagctgaca
    19441 aactaacagg tggtaaaaat attgtaaatg cgatacttgg tgactttaga gcattagata
    19501 cattatttga aggattagtg ttaattatta ctgggctagg tatttacaca ttattaaatt
    19561 atcaagatcg gaggggacaa gatgaaagag aatgatgtcg tgttaagaac ggtcacgaaa
    19621 cttgttgtat ttattttatt gactttcgga ttctatgtct tcttcgcagg tcataataat
    19681 cctggtggtg ggtttattgg tggtttaata tttagttcag cgtttatttt aatgtttctg
    19741 gcttttaatg ttgaagaggt tttagaaagt ttaccgattg attttagaat tttaatgatt
    19801 attggagcat tggtatcatc tattactgcg ataataccta tgttttttgg aaaaccattt
    19861 ttgtctcaat atgaaacaac ttggatactt ccaattttag gacaaattca tgtaagtaca
    19921 ataacacttt ttgaattagg tattttattc tcagttgttg gtgttattgt cacagtgatg
    19981 ttgtcgctta gcggaggtcg atcatgaatt taatattatt actagttata ggatttttag
    20041 tgtttatagg aacatatatg attttatcaa tcaatttaat tcgtattgta atcggaattt
    20101 caatatatac tcatgctggt aatctcatta ttatgagtat gggaacgtat ggttctagta
    20161 gatcagaacc actaataact ggtggaaacc aattgtttgt tgatcccttg ttacaagcta
    20221 ttgtactaac tgcaatagtt atagggtttg ggatgactgc gtttttactt gtacttgttt
    20281 atagaactta taaagtaaca aaagaagatg aaattgaagg cctaaggggg gaagatgatg
    20341 ctaagtaact tattgatttt accaatgtta ttaccattcc tttgtgcctt aatccttgta
    20401 tttttaaaaa ataatgatcg tatttctaaa tatttatact taggtacaat gactatcacc
    20461 acaattattt cattaatgct attaatttat gttcagcgtc accgtccaat tacgctagac
    20521 tttggaggat ggtcagcgcc ctttggtata cagtttttag gagattcttt aagtttaatt
    20581 atggttacaa ccgcttcgtt tgtgattact ttaattatgg catacggatt tgggcgtggc
    20641 gaacataaag caaatcgtta tcacttgcca tcgttcatat tatttttaag tgttggcgtg
    20701 ataggctctt ttctaacatc agatttattt aatttatacg tcatgtttga aattatgtta
    20761 ctagcgtcat ttgtactcat tacacttgga caatctgtag aacaattacg tgctgcaatt
    20821 atttatgttg tcttgaatat tattggttca tggctattct tattaggtat aggtttactt
    20881 tataaaacag taggtacatt aaacttttca catattgcaa tgcgtttgaa tgacatggga
    20941 gataatcgca ctgttacaat gatttcatta atcttcttag tcgcatttag tgcgaaagca
    21001 gcgctggtcc tttttatgtg gctacccaaa gcctacgctg tgttaaatac tgagcttgca
    21061 gcattatttg cagcgttaat gaccaaagta ggggcctatg cattaattcg attcttcact
    21121 ttactatttg atcaacataa tgatctcata catccattgc tagcaactat ggctgctata
    21181 actatggtca tcggcgctat aggtgtcatt gcttataaag atattaaaaa gattgcagct
    21241 taccaagtca taatctcaat aggatttatc attttaggtt taggaacaaa cacgtttgca
    21301 ggtattaatg gtgcaatatt ttatttggta aatgacattg ttgtaaaaac attgctattt
    21361 tttattattg gtagtttagt ttacattaca ggctatcgac aatatcaata tttgaatggc
    21421 ttagctaaaa aagaaccttt atttggagtt gcgtttatta taatgatttt tgctattggc
    21481 ggcgtgcctc catttagtgg ctttccgggg aaagtactta ttttccaagg tgcattgcaa
    21541 aatggcaatt atattggact agcgttaatg attattacta gtctaattgc aatgtacagt
    21601 ttatttagga tactttttta tatgtatttt ggagataaag atggggagga agttaatttt
    21661 aagaaaatcc cgctatatcg aaaaagaatt ttaagtattt tagtagttgt ggttatcgca
    21721 atcggaattg ctgcacctgt tgtgttaaat gttacaagtg atgcaactga gttgaacacg
    21781 agtgatcaat tatatcaaaa acttgtaaat ccgcatttga aaggagagga ctaaatgaat
    21841 caaatagttt taaatattat cattgcattc ttatgggtat tatttcaaga tgaagatcat
    21901 tttaaattct cgactttctt ttctggatat ctaattggtt taattgtcat ttatatatta
    21961 cacaggtttt tcagcgatga tttttatgtt agaaaaatat gggtagctat taaattttta
    22021 ggtgtttatt tatatcaatt aataacatct agcattagca cgattaatta tattcttttt
    22081 aaaacaaaag atatgaaccc tggattactt tcatatgaaa caagactaac aagtgattgg
    22141 tcaataacat ttttaacaat tttaattatt ataactccag ggtctacagt aatacgaatt
    22201 tctcaagact ctaaaaagtt ttttattcat agtatcgacg tgtcagaaaa agaaaaagat
    22261 agtttgttaa gaagtattaa gcattatgaa gacttaatat tggaggtgtc gcgatgatac
    22321 aaacaataac acatattatg attattagtt cactcattat ttttggaatt gcattaatca
    22381 tctgtttatt tagattaatc aagggaccta caacagcaga tcgtgtcgtt acatttgata
    22441 caacaagtgc tgtcgtaatg tcaattgtgg gtgtgttaag tgtacttatg ggcaccgttt
    22501 ctttcttaga ttcaatcatg ctcattgcca ttatatcttt tgtaagttct gtttcaatat
    22561 cacgctttat tggtgggggg catgtgttta atggaaataa caaaagaaat ctttagtctt
    22621 attgctgctg tgatgttgtt gttaggtagt tttattgctc ttattagtgc aataggtatc
    22681 gtgaaattcc aagatgtttt cttaagaagt cacgctgcga caaaaagttc aactttatcc
    22741 gtgttattaa ctttaatcgg tgtattaatt tattttattg tgaatacagg atttttcagt
    22801 gtgcgtttat tactgtcact tgtttttatt aatttaactt caccagtcgg catgcactta
    22861 gtcgctcgcg ctgcttatcg caacggcgct tatatgtatc gaaaaaatga tgctcacaca
    22921 catgcatcaa tattattaag ttcaaatgaa caaaactcta cagaagcatt acaattacgt
    22981 gctgaaaaac gagaagagca tcgtaagaaa tggtatcaaa acgattgaag tgttggctaa
    23041 attgttaagc ataacaatgc ttttgaaaat ctgttttcaa aattaatcat taaaagagta
    23101 ctttgaaatt ggttagtcga ttttaaggtg ctcatttttt gtattataag ttaatgttgt
    23161 tatgtgaaag caagttgttt atttatacaa acaattagtg ataaaataaa attaaataat
    23221 tgcaaattta agtactgcaa tcgtagacat atatctatat taaagtgctt ttgtggtcgc
    23281 taactcaatt tatcctctaa agtataatta caacgagatg tgatacttaa ttttcaatta
    23341 aacaatactt tttagagagg tgaaagtttg gaaatatttg aaacaattct tatatttata
    23401 gctgttgtga tactaagttc gtttgtccat actttcatac ctaaagtacc cctagcattt
    23461 atacaaattt tcttgggcat gttactattt attaccccaa tccctgttca atttaatttt
    23521 gattctgaat tgtttatggt aacaatgatt gcgcctttgt tatttgtaga aggtgttaat
    23581 gtttctagag tccatttaag gaaatatatt aagccagtga tgatgatggc attaggatta
    23641 gtcattacta ctgtgatagg tgtaggttta tttattcatt ggatttggcc agatttacct
    23701 attggagcag catttgcaat tgctgccatt ctttgtccta ctgatgcagt agcagtgcaa
    23761 gcaatcacta aaggaaaggt cttgccaaaa ggagcaatga caattcttga aggtgagtca
    23821 ttattgaatg atgctgctgg tattatttca tttaaaatag ctgttggagt attagttaca
    23881 ggtgcttttt cacttgttga tgctgttcag ttgtttttaa ttgcatcaat tggtggcgca
    23941 gtggttggtt tacttatagg tatggcatta gtaaggttcc gattaacatt gatgcgtcga
    24001 ggatatgaaa acattaatat gtttacaatt attcaattgt taacaccatt tgttacgtat
    24061 ttaattgctg aattgtttca cgcatcagga atcattgcag cagtagttgc aggacttgta
    24121 catggtttcg aacgtgacag aattatgcaa gtacgtacac aactgcaaat gagttacaat
    24181 catacatgga atatactagg ttatgtttta aatggctttg ttttttcaat attaggattt
    24241 ttagtacctg aagttattat taaaattatc aaaacagaac cgcacaattt aatcttttta
    24301 ataggcatca ctattgttgt tgctttagct gtctatctat ttagatttgt ttgggtttat
    24361 gtcttatatc cttattttta tttagccatc agtccattcc aaaaaatgat gactaaaaat
    24421 gatgatgata atccaacgac tgagaaacca ccaaagcgaa gtttatacgc tttaattatg
    24481 acgttatgtg gtgtgcatgg aacaatttct ttagcaatcg cattaacgtt accgtatttt
    24541 ttagcagggc atcatgcttt tacgtataga aacgacttat tatttattgc atctggtatg
    24601 gttattatta gtttggtagt tgcgcaagta ttattgccat tattaacgaa acctgcacct
    24661 aaaacagtaa ttggcaatat gtcgtttaaa gttgctagaa tttatatatt agaacaagtt
    24721 attgattatc taaatcaaaa atctactttc gaaacaagtt ttaaatatgg taacgtgatt
    24781 aaagaatatc atgataaatt agcattttta aaaactgtag agaaagatga tgaaaactct
    24841 aaagaattag aacgtctaca aaaaattgct tttaatgtag aaacaaaaac attagagtct
    24901 ttagtagatg aaggacaaat aacgaatagt gtacttgaaa actatatgcg ttatgctgaa
    24961 agaacacagg tatatagaca agcatcatta ataagaagaa tgattgtatt attacgaggt
    25021 gctttattaa aacgaagagt acaaacgaga gtgaactccg catcttcact tagtgttacg
    25081 gataacttaa tggaattaaa taaaattaat aaattagtcc attataatgt ggttagtcgt
    25141 ttgtctaagg aaacaacaaa agataataca cttgaagttg gaatggtttg tgacggttat
    25201 ttaatgcgaa ttgaaaactt aacaccatca aatttcttca actcagcaag tgaagatacg
    25261 attactaaaa ttaaattaaa tgcattgaga gaacaacgtc gcattttacg tgagttgatt
    25321 gatacagatg aagtatcaga aggtacagcg ttaaaactaa gagaagccat caattatgat
    25381 gaaatggtta ttgtagatag tatgacgtag ttcctaatta tgctaaaagg gattgatgaa
    25441 aaactgaagg gcttttcatc aatccctttt attttagggg aattgaatag atagttttaa
    25501 actatacgaa ttattaatat ttgagattta attgaaataa gttttaaaaa ttggaggaga
    25561 tagattaagc gaagtcattt aaaggtgaag ttaagtgtat tcacaaaaaa tagccacact
    25621 catatgacat cggatgagtg tggcttaagg atctatgggg ggaggaaacc atagatgttt
    25681 actttgatag gccagattaa atatcaaagt atgcgattat ttatagcttg atgcaaaagt
    25741 ggtatgccta ttaaaagtta ctgcacatag cttttaatat tccgttcaaa ggaaaggggc
    25801 atacaattga acaatctgta atagtacttt taaccagcta tgctaaaagt ctagtaggga
    25861 gaacagttgt ccaatcacat aagaacctct aacttcgtta gtacgattaa gaaaagcttt
    25921 ttagttagta tgtaatacaa tttattgacg cgcgtgaatc tcttttataa gagtgtgtag
    25981 ggaatggcgt tgtataaatt gtattagaag aacttctaac gcatctctgt ggttaaaaga
    26041 gatgaaggga acgacagttt aattaaaact gcataagaac ttctagcttt tctctctcgt
    26101 tcaaagagaa g
//
LOCUS       DANRDI010000020        25799 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.020, whole genome shotgun sequence.
ACCESSION   DANRDI010000020 DANRDI010000000
VERSION     DANRDI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25799
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.020"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(81..668)
                     /locus_tag="PZ377_000656"
     CDS             complement(81..668)
                     /locus_tag="PZ377_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDM6357544.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(646..744)
                     /locus_tag="PZ377_000657"
     CDS             complement(646..744)
                     /locus_tag="PZ377_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357545.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            801..2483
                     /locus_tag="PZ377_000658"
     CDS             801..2483
                     /locus_tag="PZ377_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDM6357546.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(2612..2995)
                     /locus_tag="PZ377_000659"
     CDS             complement(2612..2995)
                     /locus_tag="PZ377_000659"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDM6357547.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(2997..3848)
                     /locus_tag="PZ377_000660"
     CDS             complement(2997..3848)
                     /locus_tag="PZ377_000660"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDM6357548.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(3841..4659)
                     /gene="panB"
                     /locus_tag="PZ377_000661"
     CDS             complement(3841..4659)
                     /gene="panB"
                     /locus_tag="PZ377_000661"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDM6357549.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            4732..5592
                     /locus_tag="PZ377_000662"
     CDS             4732..5592
                     /locus_tag="PZ377_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDM6357550.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(5678..6382)
                     /gene="budA"
                     /locus_tag="PZ377_000663"
     CDS             complement(5678..6382)
                     /gene="budA"
                     /locus_tag="PZ377_000663"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDM6357551.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(6660..7619)
                     /locus_tag="PZ377_000664"
     CDS             complement(6660..7619)
                     /locus_tag="PZ377_000664"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDM6357552.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            8306..9754
                     /locus_tag="PZ377_000665"
     CDS             8306..9754
                     /locus_tag="PZ377_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6357553.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(9835..11172)
                     /locus_tag="PZ377_000666"
     CDS             complement(9835..11172)
                     /locus_tag="PZ377_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDM6357554.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            11430..11846
                     /locus_tag="PZ377_000667"
     CDS             11430..11846
                     /locus_tag="PZ377_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357555.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            11969..12859
                     /locus_tag="PZ377_000668"
     CDS             11969..12859
                     /locus_tag="PZ377_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDM6357556.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(13052..14548)
                     /gene="mqo"
                     /locus_tag="PZ377_000669"
     CDS             complement(13052..14548)
                     /gene="mqo"
                     /locus_tag="PZ377_000669"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDM6357557.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            14844..15173
                     /locus_tag="PZ377_000670"
     CDS             14844..15173
                     /locus_tag="PZ377_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357558.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(15233..16831)
                     /locus_tag="PZ377_000671"
     CDS             complement(15233..16831)
                     /locus_tag="PZ377_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDM6357559.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(17025..17468)
                     /locus_tag="PZ377_000672"
     CDS             complement(17025..17468)
                     /locus_tag="PZ377_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDM6357560.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(17749..17961)
                     /locus_tag="PZ377_000673"
     CDS             complement(17749..17961)
                     /locus_tag="PZ377_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDM6357561.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(18239..19948)
                     /gene="betA"
                     /locus_tag="PZ377_000674"
     CDS             complement(18239..19948)
                     /gene="betA"
                     /locus_tag="PZ377_000674"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDM6357562.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(20209..21699)
                     /gene="betB"
                     /locus_tag="PZ377_000675"
     CDS             complement(20209..21699)
                     /gene="betB"
                     /locus_tag="PZ377_000675"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDM6357563.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            21768..21863
                     /locus_tag="PZ377_000676"
     CDS             21768..21863
                     /locus_tag="PZ377_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357564.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            21952..22515
                     /locus_tag="PZ377_000677"
     CDS             21952..22515
                     /locus_tag="PZ377_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDM6357565.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(22703..23389)
                     /locus_tag="PZ377_000678"
     CDS             complement(22703..23389)
                     /locus_tag="PZ377_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDM6357566.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(23653..25275)
                     /locus_tag="PZ377_000679"
     CDS             complement(23653..25275)
                     /locus_tag="PZ377_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDM6357567.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            25407..25568
                     /locus_tag="PZ377_000680"
     CDS             25407..25568
                     /locus_tag="PZ377_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357568.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            complement(25584..25694)
                     /locus_tag="PZ377_000681"
     CDS             complement(25584..25694)
                     /locus_tag="PZ377_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357569.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
BASE COUNT         8099 a         4894 c         3989 g         8817 t
ORIGIN      
        1 tgttattgat aaagatatca tttaataagc ttatgttatt tagcaaggca agccactctc
       61 gattcatggc ttgccttatt ttattgtata tttttaatta tcgttgctgg cccttgattg
      121 acaatcgtat taaaatgcct tattaagtca actttgtcta tacggtttgg atcttctacc
      181 caatgtctta taaaagacaa tcccgcacct gaaacataac tcatgaaata agaaaatggt
      241 ataccattaa tttgatcatt ttcattttta taacgttgta aatgacacat aattaaatca
      301 taaagttttt cgtggatatt tgaatcttta cccatacgaa acatcaattg ataaaatgcg
      361 atgtcttttt ctatcatttc tattaaaacg gtcataattt gatgtatgtt atccgtggat
      421 aacttaactg ctccatttaa cttctcatca tgaatgaagt ctcttatttc ctccaactgc
      481 tggtcctcta atttttcaag caaatcatac ttatcataat aatgcgtata aaatgtacta
      541 cggttaacat cagctaaatc tgcaatttgt tgcacagtaa tctcttctaa ttggtgttga
      601 tgtaaaagtt caataaatgc atttctcatt gcaacttgtg attttctaat acgtcgatct
      661 atagtcattt atatcaagtc ctccccaatg attataaacg ttatgttcat tatcccacaa
      721 atctccaaca ttgatgattg gcacacaatg tttacctgtt taatataggt gatacaaaca
      781 aacagaaaaa ggtgataaca atgaaccaac atttactagg aaatccaaaa ttaactgtaa
      841 ctcatgtcaa tgaagttaaa gccggtatta accacatcgt tgtcgacagt gttcaatatg
      901 gaaatcaaga aatgattatg gaaaaagatg tcactgtgga aatgcgcgat ggcgaaaaat
      961 tatatattaa tattttcaga ccaaataaag atggcaaatt ccctgtagtt atgtctgcag
     1021 atacttacgg taaagataat aagcctaaaa tcacaaatat gggtgccctt tggccaacat
     1081 taggtaccat tccgacatct agttttacac ctgaagaatc accagaccca ggattttggg
     1141 tgccaaatga ttatgttgta gttaaagttg cattacgcgg tagtgacaaa tccaaaggcg
     1201 tcttatctcc atggtcaaaa agagaagcgg aagattatta cgaagtgatt gaatgggcag
     1261 caaatcagtc atggagtaat ggaaatatcg ggacaaatgg tgtttcttat cttgcggtga
     1321 ctcaatggtg ggtcgcatca ttaaatccac cacatttaaa agcaatgatt ccttgggaag
     1381 gcttaaatga tatgtataga gaagtagcct ttcacggagg tataccagat actggctttt
     1441 atcgtttctg gactcaaggt atttttgcga gatggacaga taatccaaat atcgaagatt
     1501 tgattcaagc acaacaagaa catcctctgt tcgatgattt ttggaaacag cgtcaagtgc
     1561 cattatcaca aattaaaaca cctctactaa catgtgctag ttggtctaca caaggtttgc
     1621 acaaccgtgg ctcttttgaa ggatttaaac aagctgcatc tgaagaaaaa tggctatatg
     1681 tgcatggacg taaagagtgg gaaagttact acgctagaga aaatctcgaa cgccaaaaat
     1741 cattctttga tttttacctt aaagaagaaa ataacgattg gaaagatacg cctcatgtca
     1801 tttatgaagt tagagatcaa ttttataaag gcgaattcaa atcagcgtca gcgttccctt
     1861 tacctaacgc agaatataca ccattgtatt tgaatgctga aaatcacaca ttgaatcatg
     1921 caaagattag tagcgcgcat gtcgcacaat atgactctga agataaacaa caagatgtaa
     1981 gttttaaata tacgtttgac aaagatactg agttagttgg aaacatgaac ttaaaactat
     2041 gggtaagcac taaagactca gatgatatgg atttatttgc aggtattaaa aagttagatc
     2101 gtcgtggtaa tgaagttaac ttccctgatt ttaatcatat tgaaaatggt caagtagcta
     2161 ctggttggtt acgcgtatca catcgtgaat tagatcaaga aaaatcctca atcgcgcaac
     2221 cttggcataa acatgaaaca gaattaaagt tgtcacaaga tgagattgta cctgttgaaa
     2281 tcgaattgtt accttcaggc acgctattta aacaaggcga aacattggaa gttgttgtaa
     2341 agggtagtga aattgtaatt ggtaatagta ctcctggcat gaaaacacgt tatgaacatg
     2401 aagaaaccgt aaataaaggc atgcacatga tttatactgg tggtaaatat gattcacaat
     2461 taatcattcc tatcgttaat tgatatgcag caattacggt cgcttttgat taaaagtgac
     2521 atagtgatag gactgtataa caagagaaag ccacacgctt ggaatcttaa accaaggtgt
     2581 ggcccttttt attattgatg gctatttaat tttataacac tatcgtattt tctttttcat
     2641 gaatcatttc aataatgaca ttatcttcat tcattactgc tactttaggt gcatggtttt
     2701 taatttcttc ttcattcaac tgtgcataag tcatgattat gactacatcg cctacttcaa
     2761 caagtcttga cgctgcaccg tttaaacaaa ttttaccact acctctttca ccagctatta
     2821 cgtatgtttc aaaacgtgca ccattattat tattcacgat ggctactttt tcatttggca
     2881 agatgtctac cgcttccaat atatctgaat caatcgtaat gctacctaca taatttaaat
     2941 ttgactcagt cactcttgct ctatgaattt tagcattcat cattgttctt atcactttat
     3001 tcagctccaa ttattatatt atctattaaa cgcgcttttg aaaatttaac agctaacgag
     3061 ataaatatgc gtccagttat ttcgtgttgt tctactaatt gaggataact ataaacagca
     3121 acttcttcaa tgcgtccact tatatgtgat tcaagatatt cagtaaccct gtctataatt
     3181 actttacttt gacgttcacc gtcttgatac aacgcttgtg ctaatagcaa acttttactt
     3241 aaatgtaccg cttcttgtcg ttcttgctcc gttaaataaa catttcttga acttttcgcc
     3301 aaaccatctg cttctcgaac gatatcaata ccaataattt caacggcatg attgaagtct
     3361 tttaccattt gctcgacaat agccaattgc tgggcatctt ttttaccaaa ataagcataa
     3421 tccggcataa caatattaaa tagcttatta actactgtta ccaccccatc aaaatgccct
     3481 ggacgcttcg ctccttctaa cacatcagct aatgggccta ctttgacatc aatacctaat
     3541 tcacctggat acatatcttc tactgcagga tgaaaaacaa tgtccgctcc tacttctgat
     3601 actaattcta aatctttatc aatttgtctc ggataagcat cgaaatcttc gtttggacca
     3661 aattgtaatg gattaacaaa tacactcaca attgtaatat catttgtact aactgattcg
     3721 cgtaccatcg ttaaatgtcc atcatgtaag gcacccattg ttgggataaa accaatcgtt
     3781 gtgcctgagc gtttggctgc tttaacaatg tgttgcatct cttttaccgt agtaatcagc
     3841 ttagtcattg ttattaacct cattcataat tttcttttta tacgtatatt cttctgatgg
     3901 aaatgcacca gatttaactt cttgatcgta ttgttttaaa ccatccacac caacactaaa
     3961 atcagcaaat tgcttcacaa atttcgcttt atgttcaaca ccataattta acatatcgtg
     4021 ataaaccaat acttgaccat ctgtaccttt tcctgcacca ataccaatga ctggaattgt
     4081 taagtgcttg ctaatttctt ctgctaaatc atttggaatt gcttcaagta ctaacgcaac
     4141 tgcaccagct tgttctacat ttttcgcgtc taaaataagt tgctccgctg cttctttcgt
     4201 tgcaccttgt aatttatacc ccataacgcc aacactttga ggtgttaatc ctaaatgtgc
     4261 aacaacagga ataccaattg ccgttgcttt ttcaataaat ggtgtaatat gcgctccttc
     4321 tgctttaatt gcatttgcat tcgtctcctg ataaagcttt agagcatgat ttaagtcttg
     4381 tgtcatagag atgcctactg caccaatcgg catatcaaca actacaaatg tatttggtgc
     4441 gcctcttctt actgcacgac cgtgatgaat catatctgct aacgtcactt gtacggtact
     4501 ttcataacct aatacagtca taccaagtga atccccaaca agaatcatat caatacccgc
     4561 tgcttccact tgtttagcac ttggaaaatc ataagctgtt accatagaaa ttttagtttg
     4621 cttttgtttc atatctatta attgacttac tgttttcaat gttattcaac ctctttttgc
     4681 agtattatta gaaatagtat aatgaatctt ttatagaaag gaaagaagaa gatgttatca
     4741 gttgcgatta tcggcccagg cgctgtcggt acaactattg cctatgaatt acaacaatca
     4801 ttgcctcata caacgcttat cgggagacac gccaaaacaa taacatatta tactgtacca
     4861 catgcacctg cacaagatat tgttgtgaaa ggttatgaag atgtcacaaa cacatttgat
     4921 gttattatta ttgcagtcaa aacacatcaa cttgatgcag ttattcctca tttaactcat
     4981 cttgcacatg aggacacgct catcatttta gcccaaaatg gttatggtca actcgaacat
     5041 atttcattta aaaacgtatg ccaagcagtt gtctatataa gtggtcaaaa gaaaggcgat
     5101 gttgttacgc acttcagaga ttatcaatta cgtatacaag ataatgcatt aactcgacaa
     5161 tttagagatt tggtccaaga cagtcaaatc gacattgtat tagaagcaaa tattcaacaa
     5221 gctatttggt ataaattgtt agttaacttg ggcattaatt ctatcacagc actcggtaga
     5281 cagaccgttg caatcatgca taatcctgaa atacgtacac tgtgtagaca gctattacta
     5341 gatggttgtc gtgttgcaca ggcagagggg ttaaactttt cagaacaaac tgtcgatact
     5401 attatgacta tatatcaagg ttatcccgac gaaatgggaa cgagcatgta ttacgacatt
     5461 gcgcatcagc aacctcttga agtcgaagcg attcaaggct ttatttatcg acgtgcacgt
     5521 gaacacaatc ttgacacccc atatcttgat actatttata gctttttacg cgcgtatcaa
     5581 caaaatatgt aagcacatat ataatataaa aagaccttaa cgatgcattt agaaccacct
     5641 gttcctaata tcgctaaggt cttttaatta tattagacta ttcagcttct ctaatttctt
     5701 ctgccacatc tttatagtct attttcgctt taacaaacgt ctcgttattt accgggaaat
     5761 gttgttggaa tgtttcaaag ttttgtatct caacgacaac gtcatccact tcaaagtcaa
     5821 gaacatgtcc accataagct ctttcatcat ccgcaaaatg tatatgaaaa ccagcagacc
     5881 ctacgccatg aaataattct ggtgtaaaaa atccaacaat ggcaccacga atatcttgtc
     5941 ttttttcctc aggttgtctg cgtgctgaat caatcaaacg tgtatatggc ggttgctgag
     6001 caggcatcat tcgtacatgc atatgtttaa atgtgccata aattttaaca gccgaaaata
     6061 aattctcact taacatttca tttttaattt gtgcaaatac atcatcttgt gataattgtt
     6121 gcaatggaaa tgtcttactc gctttaaaat tagtaatcga tgcatacggt actttctcat
     6181 cgccttttaa ttctataaat tctttatgct cgttagcatg atatgccttt ccgtctaaaa
     6241 atattacttc gccatcagac cctgttaacg ttgcaatccc taaattccca tgttctaaca
     6301 attcattaat tgtagctgtg ccttctagta agccagccat taacgtacct aatgtaccat
     6361 gttgatacaa gacattcgtc attccaaaac catctcccta tagttattcc tcgttgtcat
     6421 gctctacttc gtgattaatg cagaactact ttcctttata ctcaatgacg cctcaacatt
     6481 tttattcatg tcactaaatt ttcacgagtc ataatacttt attcaattat tattttacgc
     6541 aaaagtattg cctctatcaa tgcttgctaa aaaaatggac ctatcagtgc cacgtgttac
     6601 tgcaacacta atagatcctc ttaccattaa aatatttcta attaatactt aaaataggct
     6661 tagtcttcta ataaatattt aattgaatca aatgtatctt ctaatgtttt aacagattta
     6721 tcgaacaacg cttgttcttc ggcacttaat ggcatttcat aaattttaac tgcgccatgt
     6781 tggttaacta atgttggtac acctaggtaa acgcctttgt gaccaccata ttgaccatct
     6841 aattgtatag aaacatttaa gacattattt tcattattta aaatggcttt tgaaatgcgc
     6901 atcaatgcta atgcaatacc atagtatgtt gaccctttag cttggataat ttcataagca
     6961 gcgtcacgtg tattcacata aatttcttcc gctttagctt cgctaccagt ttgttctttt
     7021 aatgtgtcat atactgaaat acctgctaca tttgcttgtg accaaactgc aagttcagta
     7081 tcaccatgct cgccaataat actagcgtca acacttgaag gtgcaacacc aagttcttgg
     7141 ctaattaaat attgtaaacg tgcactgtcc aatacagtac ctgaaccgat aacacgctct
     7201 gctggtaatc cagtatattc ttttacaaat cttgttaaaa tgtctacagg gtttgccgcg
     7261 attaagaaat atccatcaaa gccactatcc ataacactct taacgatgct cttcataatc
     7321 ttagtatttt tttcaactaa ttgtaaacgt gtttcacctg gcttttgagg tgcaccagct
     7381 gtaataacaa ctaaatctgc atctttacag tcttcgtatt cacctgcttt cacatcaact
     7441 ggtgaaggac tgtggactgt accatggttt aaatcttgaa catctgcttt tactttgtct
     7501 tttgcaatgt caataattac aaattcatca gcaacacctt gcgtaaccat tgcaaaggca
     7561 tagcttgatc ctacagatcc atctccgatt aatacaacct ttttaccaaa tgttttcata
     7621 attatgtctc ctaacctaat tgaatttatt ttacacgtga ctacacgtaa aaatatctat
     7681 actgaaatta aagattaaca attttatttt attataatat tatgctacca aattacaaac
     7741 ataaaactga gatgtaagcg gagtcaaatt acactctatc gccaataaat attgatatct
     7801 caagctttag cacgtaattt gaaatttgtg acaattttta gcattctaaa attgcgtaac
     7861 gacgctattt aaaggcttta acaaactatt gagctttcta tttttttcat tttaaaaagg
     7921 atggctattc gacttaaatg acttactata taacctccta ttttccgcct taccctcatc
     7981 atatttgatg catcatttta tagtcaaaga attacatttc atacaaacta gccaatcaga
     8041 cataaacatg aaattgattt taaaaatata atgtgcaaaa ttgttagaaa attagcaaaa
     8101 aatagtactt attcatttaa tatctgttat aatcagtttt atcatttaaa aaatctatgg
     8161 atgttatcaa gacaagtcat taatacgtaa cataaagttg tgatttttca gaatttttag
     8221 acaaaatatt gattatttgt gctatgatat aacatattaa aaaacaccgt atagaattgt
     8281 ttatgaatgt ttgggaggaa agtttatggg aagttttttc aataaaatag cacgaaaaga
     8341 ggatccggct atctatcaaa ataaagatgg tcatttaaag cgtacacttc gggtgcgtga
     8401 tttcttagct ttaggtgtag gaacaattgt atcgacatct atctttacgc tacctggcat
     8461 tgttgctgca gaacatgcag gaccggccgt tgcgttatca ttcttactcg ctgctattgt
     8521 tgctggttta gttgcattta cttatgcaga aatggctgcc gctatgccat ttgcaggttc
     8581 agcctattct tgggtcaatg tattatttgg tgaatttttt ggatgggttg ccggttgggc
     8641 tctattagct gaatatttta tcgccgtagc ctttgttgca tcaggattct cagcgaattt
     8701 acgcggactt gtgaaaccaa ttggcatcga attacctgca gcattatcaa atccatttgg
     8761 tacaaatggc ggttttatcg atattattgc tgctatcgtt attttattaa ctgcattatt
     8821 actatcacgt ggtatgtcgg aagcagctcg tatggaaaat attttagtta ttttaaaagt
     8881 attagctatt attttatttg tcatcgtagg tttaacagca ataaatgtta gtaactatgt
     8941 gccatttatt ccagaacaca aagtaactgc tacaggtgac tttggtggat ggcaaggcat
     9001 atatgctggt gtttcaatga ttttcttagc gtatatcggt ttcgattcta tcgcagcaaa
     9061 ctcagcagaa gcacttgatc ctcaaaagac aatgcctaga ggtattcttg gttctttaag
     9121 cgttgctatc gtattattta ttgctgtagc acttgtgtta gttggtatgt tccattactc
     9181 acaatacgca aacaatgctg aacctgttgg ttgggcttta cgtcaaagtg gtcatggtgt
     9241 tgtagcagct attgttcaag ctatctctgt tatcggtatg tttacagcat taattggtat
     9301 gatgttagca ggctcacgtt tactttattc atttggacgt gacggcttat taccttcatg
     9361 gttaagccac ttaaacgaca aacatttacc taatcgcgca ctagttatac ttactattat
     9421 tggtgtttta attggttcta tgttcccatt cgcattttta gcacaattaa tttcagcagg
     9481 tacacttgtt gcatttatgt tcgtttcatt agcaatgtat cgtttgagaa agcgtgaagg
     9541 taaagatcta ccaattcctg catttaaatt acctttatat cctgtgttac cagcaattac
     9601 atttgtttta gtattgctag tattctgggg attaggattc gaagcgaaat tatacacttt
     9661 aatttggttt attgttggta tcatcctata tttaagttat ggacttagac attctaagaa
     9721 aaatgacgta gctgaatacc acccaccaaa ataaaaaata aaaaaaatca tttcccatct
     9781 gattaacatc cagatgtgga aatgattttt tattttatat taagataatc gctattacca
     9841 accttgtcca gatatgtcat attgatctaa gttacctgct tccaaagcag tcagtgcatc
     9901 ttctatagtg tttaacgccg tgtctaattg ctcatatgtt attaccaatg gcggttggaa
     9961 tcgcaacaca tttcctgcta cagctataat aactacgcca tgctcaaagc agtaattaca
    10021 aattttaagt gccgcactgg catcacgtgt tttgagtttt ttgtcggaaa caatatcaat
    10081 accaatgctc agacctttac ctctaacatc gcctacacta ttgtatttag atacccattg
    10141 atccattcgt ttcctaacat attccccttt ttccgcacta gcctgaagaa gcgactgatc
    10201 ttcaatcatt tgaattgtgg ctaatgcagc ttcacaacta actggattag cacctgttgt
    10261 aaataaatgt gctggtgctt ctaaacaatt cataatctct ttgcgtccaa caattgctga
    10321 cataggcata ccacctgcta aggattttcc gaaagtgatt aaatcaggcg taaaattaaa
    10381 atgcgagact gaactccatg tacctgttct cccaaaacct tgttgaatat catcgacagc
    10441 gattaaaata ccatgttcac gacaaatctt ttctaacgct tcaaaatacc ctggaactgg
    10501 ttctaaaagt ccaccatcgc cttgtatcgt ttcaataaca atacatgcta cttcgtcagc
    10561 aggtacatac ttcgcaaaca tttcttttaa gggtgctaaa tattcttcta ctgaattagc
    10621 ttgtggctgc tcgtacatac cacgatattt atctggaaac ggaatatgat aaaaaccatt
    10681 caataacgga ccataatgtt tgcgcatatt taaactaata gctgacatag acaatgagcc
    10741 aaaagttgaa ccatgatatg cattagtgaa actaatgata taaggacgcc ctgtatatgc
    10801 tctggcaaat ttaatgatgc catcatttgc gtctgatccg gttaatccga aggtcactct
    10861 tttttcaaaa tctccaggtg caatctcaca aagcttctta gctaaacgta ccaacggttc
    10921 atgatacata tatgctggtg tataatgaat aaatttatca acttgtgcct ttatcgcttc
    10981 tgttacttct ctaggtgcat gacctacgtt ttgagaactc gcactcgata acaaatcgat
    11041 atatgtcttc ccctcaatat caaccaatgt tgctccatat ccatgatcaa tcactaacgg
    11101 ataatattta atacgtcctg atttcgcaaa ataatgttca tcctcttgaa ttaattgatg
    11161 tgctttactc atatacattc ccctcccatt gacagtatta atgtgcaagc aaagtggcct
    11221 ctcttaaatc gattataaaa tccgatttaa aatcctgctc aaataccgtc atgcctctat
    11281 gaatactatc caatatacta ataaaattca gatttgtaaa tatattctga taattaagaa
    11341 ttttatatca atctactttg tttatgcgca taattttgtt cgattaggga attgtttact
    11401 atattccatt tgactctgag gtgatacgaa tgcgtttaaa agtattgttc catttcattg
    11461 ctgctatttt catatcattc atgttgctat ggatgaccat gttgtttgac ttaataagca
    11521 atcaatcaca tttaaaagct ttattattga atttagattt tctaattcct tctgacaata
    11581 caccatatat tctagaaata atatgtcact tactaattgg tagcgtcatt tattttgtat
    11641 ttgtgttgct gtttcatact tctaaaaggc tttactatct atgttatatc cctttatttt
    11701 tcttatttat agcattgtat ccctttttag tatttattgc gcaacgtcca atttttcaat
    11761 ttagtgtgac agaattaatc ggttggatta ttacacatat tttctttatg tcattaatgg
    11821 cgttagtcat acctagaata aaataatatt tttaattaaa ctgacaatta tttttcacat
    11881 tttatacact tttcaaggtt tatgacttga gaaatgttct catttttatc atactggagg
    11941 tgtattgaaa agttaaggag tggaaagtat gaataaagag caattagaaa aaatgaaaaa
    12001 tggaaaaggc tttattgccg cattagacca aagtggtggt agtacaccaa aagcacttaa
    12061 agaatatggt gtaaacgaag atcaatatag caatgaagac gaaatgttcc aacttgttca
    12121 cgatatgcgt acacgtgtgg taacttcacc ttcattctca ccagataaaa ttttgggtgc
    12181 tattcttttc gaacaaacaa tggatcgcga agtagaaagc aaatacactg cagattactt
    12241 agctgataaa ggtgttgttc cgttcttaaa agtagacaaa ggtcttgctg aagagcaaaa
    12301 tggtgttcaa ttaatgaaac caatcgacaa cttagacaat ttattagacc gtgcaaacga
    12361 acgtcacatt tttggtacaa aaatgcgttc taacatttta gaattaaatg agcaaggtat
    12421 caaagacgtt gttgaacaac aatttgaagt tgctaaacaa attattgcta aaggtttagt
    12481 tccaattatc gaaccagaag ttaatattaa tgcaaaagac aaagctgaaa ttgaaaaagt
    12541 attaaaagct gaacttaaaa aaggtttaga tagcttaaat gctgatcaat tagtaatgtt
    12601 gaaattaaca attcctactg aaccaaactt atacaaagag ttagctgaac atcctaatgt
    12661 tgttcgtgtc gttgtattat caggtggtta cagcagagaa aaagcaaatg aattacttaa
    12721 agataacgct gaacttatcg caagtttctc acgtgcatta gctagtgatt taagagctga
    12781 tcaatctaaa gaagaattcg ataaagcttt aggtgatgct gtagaatcaa tctacgacgc
    12841 atctgtaaac aaaaactaat ttatacatta aactattttt tgatataaac tactaaaaag
    12901 gactcataca tgtgctttaa aggcattgtg tatgagtctt ttttgattaa agttgatttt
    12961 gccgtatgta ttagattacg taaaaaaatc ggacagctga aaaatcttta agatttctca
    13021 gttgtccgat tttaaatata gctattagaa tttagttttc gtagtaacct aattctaagt
    13081 cttttgaagt ttgtttacgg atttttctca ttaatttttc gtcttcaatt aatgattcac
    13141 cgtatgatgg aatcattttc ttaattttag gtgcccattc agttttgtat tcagggaagt
    13201 tacgttctaa tacttctaac gcaactgaaa ctgaagttga agcccctggt gattcaccta
    13261 ataatgcaat tacagtgtgg tcttgtgagt taaccacttc tgtaccgaat tggatgaatc
    13321 ctttaccgtg ttcaggtgta tctttgataa cttgtacacg tttaccagca gtgtataatt
    13381 gccaatcttc attacgtgct tctggataga aagtacgtaa gtggttcata caaccttctt
    13441 ttgtcataat tacttggtca aatgagtatt taattaaagg taagttttta actgctgctg
    13501 ctaataaagt tgtaatgttg tatgttttaa cagacttgaa taaatctaag ttagaaccat
    13561 ttttcaagaa tttaggtcca acattagcaa atggtccaaa taataatgtt ctttgaccat
    13621 caatgtaacg cgtatctaaa tgaggtacag tcattggtgg tgtaccaggt ggctctttac
    13681 cataaacttt ggcatcgtgt tgttcaataa cttgtgggtt tgtacaagct aagaattgac
    13741 cactgatagg gaatccaccc aaatgtttac tttcagggat acctgttttt tgtaataatg
    13801 gaattgctcc accgccagca ccgatgaata cgtagtcagt tacttgtttg aatttctcac
    13861 cagttaggcg atttttaaca gtaacttccc attgaccatt tgataattgt tcaaaatcaa
    13921 caacttcatg gttaaattgc actgtagcat ttggatgtgc ttcaatgctt ttagccattt
    13981 tacgtgttaa ttcaccgaag tttacatctg taccttcgtc aattttactt gccgccatga
    14041 taccagggtt atcttcacgg cctttcatca tcaatggaat ccattttttc attacttcga
    14101 tgtcttcagt atattcgata ttatcgaaca tagggaaagc tttcatcgct tcgtaacgat
    14161 cttttaagaa tttaacattg tttttacctc taacataact gatgtgtggt aatggattga
    14221 taaattctct tgggttctcg atgctaccgc ttttcactaa gtgaccccag aattgttttg
    14281 aaatctcaaa ctcttcgtta atcactttcg ctttttcgat gtcgatagaa ccatcaggtt
    14341 gtaaaactgt gtagttcaac tcacataatg ctgcatgacc cgtaccagca ttatttcttt
    14401 cgtttgaact ttcgattgca ggacgatcca agcgttcgta aacgtggata ttccagtctg
    14461 gctcaatttc ttttaacatt gaaccaaatg ttgtgctaag tacaccggct ccaattaaaa
    14521 cgatgtcttt actattagac ttagccattg gtttcacctc tccaaaaatt gtaaatgtgt
    14581 atcatcaata tgaaagttac ataaaactga catatttctt taaaatatca acgccattga
    14641 taacttcctg ttttaattga tacgctgtaa caaaatacta tagttagtgc ttacatgtat
    14701 atgttaaagc aagcagtggt aaatgtaaat tataattatt cattaacttt gcaatatatt
    14761 aaatctttta ttcatagaag ataaatatca aatcaatcat aattatttga caataaatag
    14821 ctaacgattg ttttaatcta catttggctt atagcatttt aaacctatac tctattttga
    14881 tacaatataa gtgtaaaatc aatcataaaa aggatattca atatctgcat ccaagaaaaa
    14941 cattacaatt acctttaata ttgaaaatta ttttgaatct tgcacttata ggtattgtaa
    15001 ttatcgttct aacacatact gagtttcaac ttaatgccgg tgatagtaat gtgtttttat
    15061 taaactggga tattcgaggt ttaacatctt atattttctc attcttaata ctggttgcgc
    15121 ttgtttctaa actaatttta atatttataa ctagacgaca aagaacatca taaataaaca
    15181 agcagtccag tgcgtatcat cataccttac gcattggact gctttgctat gtttattgat
    15241 tgctatcttt ttgttgttgc catttttcta tttcagcatc tcgtagttca acacgacgaa
    15301 ttttacctga gtttgttttt ggtaagtcgt caacgaattc aatctctctc ggatatttat
    15361 atggtgcaac ttcattttta acaaattgtt gtagttcttt aactaacgta tcatcacccg
    15421 cagtatggtc ctgtaaaata acgaatgctt taacaatatt tcctcgtata tcatgaggtt
    15481 ttgctactac agcacattct ttaacggctg gatgattcgt taacgcatct tcaacttcaa
    15541 aaggcccaat cgtatagcct gaactaataa taatgtcatc tcgacgtcct tcaaaccaga
    15601 aataaccatc atcatctaca tgagctaagt caccagtgat gtagtattta cctgtttgcg
    15661 ctttcgccgt acgttctggc tctttataat accctttgaa aagtgctggc aaatcaagtg
    15721 gtactgcaat attccctttc gtattagcag gtacgctatt cccctcatca tctactacag
    15781 tgaccgaact acccggaatg cctttaccca ttgatccaat cctctgtggt gtatctttta
    15841 aaaagcctat aagcaaggta ctttcagtct ggccatatcc atctcttaca gttaaattaa
    15901 agtatttctt gaattgttca actacttctc gatttagtgg ctcacctgca gaaacggcac
    15961 tatgtaaatg cgttaagtca taatcattta agttctgtaa tttagccatc atacgatatt
    16021 ctgtcggtgt acaacataaa acattaattt gatatttttg aagcaattct aagtatgttt
    16081 caggactgaa ccttccatta aatacaaaag cagttgcacc tgaacctaat acagataaga
    16141 aaggactcca tacccatttt tgccaacctg gtgctgctgt tgcccaaact aagtcatctt
    16201 cattaataca taaccaatgt tttggtgcca tttgtaaatg tgcaaatccc catccatgac
    16261 aatgtgtaac ggctttagga ttgccagttg taccagatgt atatgacaga atcgccatat
    16321 catcacgcgt cgtatctgcc atttctagtt tgttacttgc gttttctttt tcagcttcaa
    16381 gtgaaatcca tccatctttt tgaccggcaa taacaaattt agttaacgca tcatattctt
    16441 taattttttc aaattcaact gtgaatggct ctagtgcaat aactgcatta atttcaccat
    16501 gtgtgatacg gtattgtaaa tctttagttc ttagcatttc agaacatgga atgattgcaa
    16561 cacctaattt taaagcagca atatataatt catacgtcgc aatagatcgt ggcatcataa
    16621 tgagtacttt atcgccttta gataaaccgt gcgatgctaa aacattacct actttattag
    16681 actgttcaat gagctgttgg taagtgactg atatatcttc gccttcagta ttatgatata
    16741 aaattgcctt tttatctggt atgtggctat atttttcgat ttccgaaata atgttatatt
    16801 tttcaggcgc gaatagagct gacttttgca taactaactt cctttcatac atccactttt
    16861 cctgtgatga acattgtaat tttataaatg aattatatac atcatacgcc tatctttaca
    16921 gaattttcaa ttaaataggg ttaaatacca aagtcctcga cactacactt tgacatgacg
    16981 tagcattcaa ggactttcaa atgattgagg gttgatatct cgggctagac catatcagct
    17041 aattcaatac gaatattgta tgataattca cgattaatta tttttacatc tgcacctttc
    17101 gaagtgccac gatgcttgtg tgtatgcttg tactcagctg aattttgcca atgataaaat
    17161 gcttgcctat tttcccacag cgtaataatg atatagtgtc taccagctgt tctaggtctt
    17221 aaaaacctta atgctttaaa tccatcaacg ttttttaaat gcttattacg tcgcaaaaat
    17281 ttttgctcga atgtttcttc ttgtccttcg ttaatatata aatgatttaa cacacataat
    17341 gaatcatttg ataaatcatt tatcgcttct agcacatcgt aatatgcagt gtcattattt
    17401 ttttgtattg tgagacaatc atccagttct tctattacat aacttctata ttcatcataa
    17461 attttcataa taaatgcctt catttcattt atatttttgg tcatattact ttatatctat
    17521 tactaattca ttcccgtatt tattaattac aatcatagtt tggcttcttt ttaaaagata
    17581 agactttgta aaaagtatta atatttcatg caaatggggg acaggagtcg cccactattt
    17641 ttgtgtcttc aatttcatga tcattattta acattagtca tgaaaatagc cgacaacttc
    17701 atctgtgaaa tcaccggcct tttattttag ctaactttat ttctgatttt acgattttaa
    17761 ttgatcatac agagaaagtg atctttttac aatttctaaa aactcatgat ctatattgga
    17821 catttgatga aaataagaca aaatgttttc tgttagcttc tcttgttttg ggaatgaatc
    17881 atcttcttta atccaaatcg ctaattcgcc taatggtgtt ttatcatctt taaatgtttg
    17941 tatatattcg taaaagctca tagtattcct tctctcaatt tacttatata aatcctacca
    18001 cgaaagcttt caagaaaaca caattaaatg tctatttagt gaacttttta aggttgtgca
    18061 ctcttttaat gtctgccaat taggtcaatt aatcatcaca atgtacaatt aactctattt
    18121 tcagttcata tactcacaca ccgtttttga acaacacatt aacttctcat ttagataaaa
    18181 cgcaaaaaag cctggcacca atacaataga tgccagacta agagtctact atataaattt
    18241 atttagcgta tggttttact tcgattgcac cttcattttc atcatgaaca ccatgcttat
    18301 aataatcaat atattgtggc tctaaaggct ttctgccacg tataatgtct gctgcttttt
    18361 cagctaacat taaaacaggt gcgtgtatat tgccatttgt cgtacgtggc atagctgatg
    18421 catcaactac acgtaaattt tccataccgt ggactttcat tgttaacggg tcaactactg
    18481 ccattggatc tgaagcagga cccattttag cactacaaga tgggtgtaat gctgtttcac
    18541 catctctacg aacccaatca agaatttctt cgtctgtttg cacttctggt cctggtgaaa
    18601 tttctccacc attgaatgga tccattgctt tttgagataa gatatttctt gctacacgaa
    18661 ttgcttctac ccattctttt ttatcttctt ctgttgataa ataattaaag cggatacttg
    18721 gtttttcgaa tggatcttta gatttgattt tcaagctacc acgagagttt gaatacattg
    18781 gtcctacgtg aacttgataa ccatgtgcga ccgctgcctt ttgaccatca tatcttacag
    18841 ctattggtaa gaaatggaac attaagttag gataatcaac ttcgttattt gaacgtacaa
    18901 atccgccacc ttcaaaatgg ttagatgctg ctgcacctgt acgtgtgaaa atccattgta
    18961 aaccaataaa tggcatgcgc ttgatatcta agcttggctg taatgataca ggttccttac
    19021 atttatgttg aatgtatacc tctaagtgat cttcaaagtt ttcacccaca ccaggtaaat
    19081 gaacacgtgg ctcaatgcct tttgatttta ggaactctga atcaccgata ccagataatt
    19141 gtagtaattg tggcgtattg aatgccccac cagacaaaat gacttcatta gcatcgatgg
    19201 tatgtagttt gccatttttc ttatacgtaa caccagttgc tcttctacct tcataatgaa
    19261 tttcagttac aaaggcacgt gtttcaacgg ttaagttttt acgcttcatc gctggatgta
    19321 aatatgctct tgaagctgac attcggcgac cacgatgtac ttgactatcg aacggtccaa
    19381 aaccttcttg tctaaatcca ttcacatcag gtgttttatg atagcctgct tcaacacctg
    19441 catcaaagaa tgactggaat aaaggattcg ttgctggccc tcgttttaac ttaattggtc
    19501 catcatggcc tctaaattta tcataaggcg ctgcaccgta tgttttttct aattttttaa
    19561 aatacggtaa acagtgcgca aaatcccaag tttccatacc ttctggttct gcccagcctt
    19621 catagtccat tggattacca cgttgataaa tcatgccatt aatcgaactt gatccaccta
    19681 aaactttacc tcgggcatgt gctactttac gaccgcccat atgtggttct tcatctgttg
    19741 aataaatcca atcgtaaaat ttattgcctg aagggaacat taacgcagca ggcatttgga
    19801 taaataaatc ccaaaaataa tcactgcgac ccgcttctaa tactaagact tctttatctt
    19861 tatcttcact cagacgatta cctagtacag aacctgcact gcctcctcca atgatgacat
    19921 aatcatatga tttgtttttg ttactcatcg ttatgacctc caaagtttgt tgtatttcaa
    19981 agtgagtatt gcatgaaata tttgttagtg gctgattggt tatcgttgaa ttgctaaact
    20041 tagctaacca aaacactcgc ttttcctttt acgattgtta ctaacagtaa tgaaataatc
    20101 tatgattcat tttgcgttta gtccaaatta tcaatcaaca tttcgccact tatacaaaac
    20161 aaagtgagtg tcttgtgttc ttacaatggc actcacctta tctaattttt atttgctaaa
    20221 ccaattcact aattgtggat ttgtatttgt taaaatgtgt tttgaaacaa ggtactcttc
    20281 taagccttct ttgcctaatt ctctaccgat acctgattgt ttgtatccac cccatggcgc
    20341 ttgtgcaaaa tatggatgga aatcattaat ccacaccgtt ccaagtttca acttgttagc
    20401 aacgcgttgt gcttttccaa tatctttaga aaatacagca cctgctaaac catatataga
    20461 atcattcgct aattgaatcg cttcttgttc agtttcaaag ccttctacag taacgacagg
    20521 tccgaaaacc tcttcttgta caatacgcat tgacgtatca caatttgtaa tgactgttgg
    20581 ctcgaagaat agaccatctt ttaaatcatc tctatctgga cgtttaccac caacagcaat
    20641 tgttgcgcct tctgctttag ctacatccat ataagattcg atcttattac gatgttctgt
    20701 tgaaatcact ggtcccattt cagtatcagc atcaaaacca ttacctaatt tgattttttt
    20761 cacgcgatca ataagtgctt gctcaaattt gtctttaata ctgttttgta ctaatattct
    20821 tgatcctgct gaacaaactt gacctgcatg gaaatatcca ccatttaacg cttggtctac
    20881 tgccaattca aaatcagcat catcaaagat aatgtttgga tttttaccgc caagttccaa
    20941 ggcaatattc gtaacattat tagcagcatt tttcataata tgcttaccag tctcaatgcc
    21001 acctgtaaat gatacaaggt caacctcttt atgacctgac attacgtcac caacttcaga
    21061 acctgcacct agaataagat taattgttcc tttagggaaa ccaacttctt ccattaattc
    21121 aaaaacacgt attgttgtta atggtgtaat ttcacttggt ttcataacta gtgaacaacc
    21181 cgtagcaagc gctggcgcaa ttttccatga tgcttgtaat aacggataat tccaaggtgt
    21241 aatttgtgta actacaccta ctggttcttt aacaattttg ctttctgtat ctggaattgg
    21301 tgaatcaatc atttcgccac cgtctttatc tgctaatcca gcaaaataca taaacacatt
    21361 atgaatatca tccatatctg catatgattc ttctaacgtt tttccagtat ctaatgtttc
    21421 taatcgtgct aacgcttcgc gatgttcttt aatcttgtcc gcgatggcac gtactttttt
    21481 acctcttgtt tcagcagttt cttgcgacca ttcaccagac tcaaacgcac gtcttgcagc
    21541 taagattgca cgttctgcat cctcttttgt cccttcagaa accgtaaata tcacttcttg
    21601 attgtaagga ttgataatat ctcttgtatt tttattcgcg ctttcaaccc actcaccatc
    21661 aatatattga cgctgagata aatgttttaa aagttccatt caacaacctc cgttaagttt
    21721 gttaaatttt tatttaacga attttacaaa cttatgttag catagaaatg aaaaatgtgt
    21781 caatttacaa aacaactcaa gaattaagtt ttgtaagttc gttagatttt tatttaacga
    21841 aacatttaac tttaatgctt taaatatttt taatgtcttt ttttagaaaa ggttttataa
    21901 tgtattagta tttattttaa cgtgaccaaa atgatgtacg gaggtgcaat gatgactcgt
    21961 tctcagaaca aacagcaaca attagaacaa gcaaaagata ttgtaattaa ttcaattgga
    22021 cagacaatgg atttgtatgg cactaataga agtgtaggaa acttatatgg cacaatggtg
    22081 tttgaaggca gtatgacgct tgatgaaatg cgtcatcaat tgcaaatgag caaaccaagt
    22141 atgagtgctg gcgttaagaa attgcaggaa tttgatattg ttaaacaaca atttacgaga
    22201 ggaagccgca aacaacattt tatcgccgag aaagactttt tcatcttttt ccgaaacttt
    22261 ttcactaaaa aattccagcg tgaaattgac atcaatgtcg aagcagtaaa agatgctcaa
    22321 gctattatca attcattact tgaaagcagt gatttaactg aagctgaaac acaagaggca
    22381 caaaaaatta aatctcagct tgatcataca catgtttatt acgaatggtt agaacagtta
    22441 accgaagcga ttgaaagcgg tgaaattttc aaatatttcc caattccaga acagccttct
    22501 gattctgaaa attaaaattg actcaaacaa cgacaatgat gattatgcat gataaaaaca
    22561 atgcaatagg tccacgaaat aaacggtaat ttctatctca ataaatagaa attaccgttt
    22621 ttgttttata tgacaatcaa ttatggaggt attttctagg gctttcttag attcgttaca
    22681 ttgcttcgcc aatatatttc aattagctat tctcgccctc acgtactcta tcaataatat
    22741 tttgtatttg ttcacgtttt tcatattcat tttctacaaa ataaactaac ggttcatctt
    22801 tgatagtaac ccaaggtaca agtacatcaa aatctaattc tttaaccatc tctaaacttt
    22861 gaatagattt ttctctatca cttgaaccta atataactgt acgccatctc ttcccttcaa
    22921 aacatataaa atctccagta aataagtaac ggtgatgacc atcatcccat aaaaatagtg
    22981 tcgtccctgg tgtatgtcca ggcgcgggaa taacttctaa atccttatcc aacattacac
    23041 gattactaaa ttgtttttga acagaaatct tatgtttcag tgcagccaca tcattttcat
    23101 gtataaagta aggggcttca acttgattag aacctcctat agattcatga tcatgattca
    23161 tcaatacttt tgaaacacca cctaatgatg caatgtcttg ttgggagtca cctaagtgtc
    23221 cggaatgata gataacaata ttacctgttt cacgttttaa aataaaagat ttaaatagaa
    23281 atcgattatc aaaaggcagt tccgaagtag gtgtcgcata taagtttttt gtgatggatt
    23341 ctaaactgtc atgtaatttg gactgtttat ttttaaattg attagtcatt ttatttcatc
    23401 cttcgattaa tttgaattgt acatacgcag taccctttat ttaatttaat aatcaatgag
    23461 agtctttcaa acttcgattt agaatatgct tcatacaata aataagtact tggagcacta
    23521 aaaaattaaa tcttttagta caacattaat gaaaacctaa aagttcatcc tacaatgcta
    23581 ctaaaaaaag gggaatggaa cagaaatgat attttcacaa aattcatttc gtcgtcccag
    23641 acccgctttg aattataaat tatcgtcttg ttcttcttct gatacttgaa cgattcgcaa
    23701 tgaacgacgt tcaacttctt ttaatttttc agcacgcgtt tcaagtttaa ttctatcgcg
    23761 ccctaaaatg attaaaaatg atatcatcat gaaaataaaa ataacaatta atggcacact
    23821 tgccagtatt gaagcagttt tcaatacttc taatgcacgt tcaccaccaa ctagcatcaa
    23881 tgaaaatggc aataagcaca atgcaaatgc ccagaataaa cgattggcac gtaatggttc
    23941 gcctaccact tttttctgag atgctgccgc taaaatatat gaacccgaat caaatgttgt
    24001 tgctaagaat aagaaagcag atactaagaa tagtacaatc atcaatgatg ggaatggtaa
    24061 atgatgcacc acttcaataa tggttgcctc tgtaccatgt gtatttaaat attgtgttac
    24121 attaaactgt ccagaaattt gtaaatacac agcatagtta ccaaaaatac caaagaataa
    24181 tacgcatcca agcgttccat aaataattgt tcctagcacg acttctttaa ggcgtcgacc
    24241 ttttgaaatt ctagcgataa ataaaccgat aaatggcgca tatactaacc accatgacca
    24301 gtagaatatt gtccagtctt gtgggaaatt cgtttctttt cgacctttaa taccaccgaa
    24361 tggttctaac catgttgcca tatgaaagaa atctctcaac atatttccga accctgtcac
    24421 tgtcgtttcc ataataaaaa cagtcggtcc aataataaat ataaaggcta aaagtacaaa
    24481 ggatagccaa acgttgatat cacttaactt ttgaatacct tttttcaatc ctgtatatga
    24541 actaatggca aatataaccg tgattgttaa taaaatggcc gaacgtaaaa tcatattttt
    24601 accatctaaa ccagttaatc tttctatgcc tgcagaaatt aatggcacac ctaacgctag
    24661 tgatgttgcc gcaccaccta gcaatccaaa gataaataag atatctacaa ctttacctac
    24721 aaatttatct gtttgacctt ttaaaatcgg acgacaagct tgactaattt tatacaccgg
    24781 ttgtttttta acaaatacta aataaccaat tggtaatgct ggtagaacat aaatagccca
    24841 agcaattggc ccccagtgga acataccata ttgcgtcgca tattggagtg cttcatcact
    24901 catacttttc gcgccatttg gtggaacttg atagtaaaaa gcccattcaa taacgcccca
    24961 gtataaaata tcagagccta tgcctgcaca aaacagcatt gccgcccatg taaatgtatt
    25021 aaattctggt ttatcacttg ctttaccaag tgtgacatta ccatatttac caaatgcgat
    25081 atacattaca aagcaaaaaa tcgccagccc cataaataaa tatatcgaac caattgaatc
    25141 agaaatggca ctattaatac cagtgatgat atcttcactt gcttttggaa aagccatcat
    25201 aggtataact gcaaaaagaa gtacagctac tgtccctata aaggtcgtcc agtccataac
    25261 tttctctttt ttcaattgtg ctccccctaa ttattaattt tatgaatcct gtttcgattt
    25321 atctcaaaat gtaataatta tattgattca caaaattgac aataactaac atttaataat
    25381 aatgcaaatt tcatacaatt tgaaacttgg caattattga atatttatat aatttttccc
    25441 gtaataaaca aaaccttaat agcgctaaaa taacagtgtt aagttacgat ttaacgaatt
    25501 taacaaattt tactagaatg gcatttaaga atatttatac gttattaacg aatatttatt
    25561 tattgtaaaa cgctaccaaa aagttagact tccttcccac taaaatacca cttttctctt
    25621 tcaacttttt taaaaaacgg atatgcaact tttagtattg gtatcaaaat gattgttagg
    25681 tcatattcta tcaatatatt tttataaaga attgctttta ttaactttca attatgtacc
    25741 taacctaaaa agaagccaag gcaacgaatg ttaccttgac ttctaataca tattcaact
//
LOCUS       DANRDI010000021        25783 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.021, whole genome shotgun sequence.
ACCESSION   DANRDI010000021 DANRDI010000000
VERSION     DANRDI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.021"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..860)
                     /gene="folD"
                     /locus_tag="PZ377_000682"
     CDS             complement(<1..860)
                     /gene="folD"
                     /locus_tag="PZ377_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDM6357570.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1061..1543
                     /gene="purE"
                     /locus_tag="PZ377_000683"
     CDS             1061..1543
                     /gene="purE"
                     /locus_tag="PZ377_000683"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDM6357571.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1530..2654
                     /gene="purK"
                     /locus_tag="PZ377_000684"
     CDS             1530..2654
                     /gene="purK"
                     /locus_tag="PZ377_000684"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDM6357572.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            2658..3362
                     /locus_tag="PZ377_000685"
     CDS             2658..3362
                     /locus_tag="PZ377_000685"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDM6357573.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3362..3625
                     /gene="purS"
                     /locus_tag="PZ377_000686"
     CDS             3362..3625
                     /gene="purS"
                     /locus_tag="PZ377_000686"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDM6357574.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            3627..4298
                     /gene="purQ"
                     /locus_tag="PZ377_000687"
     CDS             3627..4298
                     /gene="purQ"
                     /locus_tag="PZ377_000687"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDM6357575.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4291..6480
                     /gene="purL"
                     /locus_tag="PZ377_000688"
     CDS             4291..6480
                     /gene="purL"
                     /locus_tag="PZ377_000688"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDM6357576.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            6459..7943
                     /locus_tag="PZ377_000689"
     CDS             6459..7943
                     /locus_tag="PZ377_000689"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDM6357577.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            7936..8964
                     /locus_tag="PZ377_000690"
     CDS             7936..8964
                     /locus_tag="PZ377_000690"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDM6357578.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            8967..9533
                     /gene="purN"
                     /locus_tag="PZ377_000691"
     CDS             8967..9533
                     /gene="purN"
                     /locus_tag="PZ377_000691"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDM6357579.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            9548..11026
                     /gene="purH"
                     /locus_tag="PZ377_000692"
     CDS             9548..11026
                     /gene="purH"
                     /locus_tag="PZ377_000692"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDM6357580.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11048..12295
                     /locus_tag="PZ377_000693"
     CDS             11048..12295
                     /locus_tag="PZ377_000693"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDM6357581.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(12560..13366)
                     /locus_tag="PZ377_000694"
     CDS             complement(12560..13366)
                     /locus_tag="PZ377_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDM6357582.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13359..14759)
                     /locus_tag="PZ377_000695"
     CDS             complement(13359..14759)
                     /locus_tag="PZ377_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6357583.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(14774..15349)
                     /locus_tag="PZ377_000696"
     CDS             complement(14774..15349)
                     /locus_tag="PZ377_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDM6357584.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(15426..15526)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15933..16067)
                     /gene="graF"
                     /locus_tag="PZ377_000697"
     CDS             complement(15933..16067)
                     /gene="graF"
                     /locus_tag="PZ377_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDM6357585.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16219..17514
                     /locus_tag="PZ377_000698"
     CDS             16219..17514
                     /locus_tag="PZ377_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357586.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGDFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            17939..19111
                     /locus_tag="PZ377_000699"
     CDS             17939..19111
                     /locus_tag="PZ377_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDM6357587.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            19165..19707
                     /locus_tag="PZ377_000700"
     CDS             19165..19707
                     /locus_tag="PZ377_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDM6357588.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            19861..20127
                     /locus_tag="PZ377_000701"
     CDS             19861..20127
                     /locus_tag="PZ377_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDM6357589.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            20130..21848
                     /gene="ptsP"
                     /locus_tag="PZ377_000702"
     CDS             20130..21848
                     /gene="ptsP"
                     /locus_tag="PZ377_000702"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDM6357590.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(22085..22318)
                     /gene="nrdH"
                     /locus_tag="PZ377_000703"
     CDS             complement(22085..22318)
                     /gene="nrdH"
                     /locus_tag="PZ377_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDM6357591.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            22516..23877
                     /locus_tag="PZ377_000704"
     CDS             22516..23877
                     /locus_tag="PZ377_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDM6357592.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            23874..24893
                     /locus_tag="PZ377_000705"
     CDS             23874..24893
                     /locus_tag="PZ377_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDM6357593.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            25026..25688
                     /locus_tag="PZ377_000706"
     CDS             25026..25688
                     /locus_tag="PZ377_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDM6357594.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT         8929 a         3683 c         4849 g         8322 t
ORIGIN      
        1 tacgaatcaa tacctcgacg cattttttct gcaagcaaag tattatttaa taccatagta
       61 attgttaatg gaccaacgcc accaggaact ggtgtaatag ctccagcaat ttctttaacc
      121 gcatcataat caacgtcacc ttttaatttg ccattttcat ctggcgtatt gccaacatcg
      181 ataattactg ctccttcttt gaccacatct tttgttacta aaccaggctt accaactgca
      241 ctgacaatga catcagcatc ttttaaatat gatgccatat cttttgaacg agaatgtaag
      301 attgttactg atgcattttt ttgaagtagt aacttagaaa ctggttgtcc gacaatatga
      361 cttcgtccaa ttacaactgc atttttacct tctaaatcaa tatcagcatg ttttaatatt
      421 tccatgatgc cgagcggtgt gcaaggtaca aaagtttgtt catcgatata taatttccct
      481 atatttattg gatgaaaacc gtccacatct ttttcaggat tgattgcttc taatattttc
      541 tgttcgctaa cttgttttgg taatggtact tgtaccaaaa taccacttac agaatcatca
      601 ttatttagtc tatttagttc gtttaatact tcttcttcag tagctgtttc ttccaaatgt
      661 acgatttctg aaatcatacc aattttttca gctgctttct tttttgatct aacataactt
      721 tgactagcgc catcattacc aactaatata acggataatt taggtgtaaa acccttttct
      781 tttagcgctt caacttgatc ttgtaacccc tgtctgtagt ctttggcaat ttgtttacca
      841 tctaaaattt tagcaaccat aaaaacttct cctcctattg tcaatatcct aactttgtta
      901 gtttataaaa ctaatttaca tagcaaaaga cgtaaattca aacaaaaaat caaaataaag
      961 ttcgattttt gattgaaaaa gcagaaattg cttgttatgc tatatctata atatacaact
     1021 aaagtaattc acaattcaaa cttttgaaag ggtgtacaaa gtgaaagtag cagtcattat
     1081 gggcagttct tccgattgga aaattatgca agagagttgt aacatgttgg attattttga
     1141 aattccgtac gaaaaacaag tagtatccgc acatcgtacg ccaaaaatga tggttcaatt
     1201 tgcttctgaa gcgagagaaa gaggtataaa cattatcatt gcaggcgctg gcggtgcggc
     1261 acatttacca ggtatggttg catcattaac gacgctacca gttattggag tgccgattga
     1321 aacaaaaagt ttaaagggta tagattcttt attatcaatt gttcaaatgc caggaggtat
     1381 tccggttgca acgactgcaa ttggtgcagc aggtgctaaa aacgcaggta tacttgcagc
     1441 aagaatgtta agtattcaaa atccttcttt agttgaaaaa ctaaatcagt atgaatcttc
     1501 gttaattcaa aaagtggagg acatgcaaaa tgaacttcaa taaattaaag tttggtgcga
     1561 ctattggcat tattggtggt ggtcagcttg gaaagatgat ggcacaatca gctcaaaaaa
     1621 tgggttataa agtggttgta ttggatcctt ctgaagattg tccatgtaga tacgttgcac
     1681 acgaatttat acaagccaag tatgacgatg aaaaggcact caatcaatta ggacaaaaat
     1741 gtgatgtgat tacttatgaa tttgaaaaca tttcagccca acaattaaaa ctattatgtg
     1801 aaaagtacaa tattccgcaa ggttaccaag ctatacagtt attacaagat cgcttaactg
     1861 aaaaagaaac attaaaaagt gctggtacca aagttgtccc gttcatttca gtaaaagaat
     1921 ctacagatat tgataaagca attgaaacat taggatatcc ttttattgta aaaactagat
     1981 ttggtggcta cgatggcaaa ggtcaagttt taattaacaa cgaaaaagac ttacaagaag
     2041 gttttaaatt aattgaaact agtgaatgcg tagctgaaaa atatttgaat atcaagaaag
     2101 aagtatctct tactgttaca agaggaaaca acaatcaaat cacttttttc ccattacaag
     2161 aaaatgagca tagaaatcaa atacttttca aaacaattgt tccagcgaga atagataaaa
     2221 cagctgaggc gaaagagcaa gttaataaaa ttatccaatc gattcatttc attggaacat
     2281 ttacagttga attttttata gatagtaaca accaattgta tgtgaacgag atagcaccaa
     2341 ggcctcacaa ttccggacat tattcaattg aagcatgtga ttattcacaa tttgatactc
     2401 atattttagc agttaccgga caatcattac caaattcaat tgaattatta aagccagcag
     2461 tcatgatgaa cttactaggt aaagatttag atttattgga aaatgaattt aatgaacatc
     2521 cagagtggca cttacatatt tatggtaagt ctgagcgtaa agatagcaga aaaatggggc
     2581 atatgactgt actaacgaat gatgtaaacc aaactgaaca agatatgtac gctaaatttg
     2641 aggggagtaa ttaaagcatg acattattat atgaaggaaa agcgaagcgc attttctcaa
     2701 caaatcaaga aaatgaatta agagttgaat ataaagatga agttactgct ggaaacgggg
     2761 ctaagaaaga cacaatggca ggtaaggggc gattaaataa tcaaattact tctattatat
     2821 ttaaatattt acaagaaaat ggaatagaaa gtcactttat taaacaatta tctgaaacag
     2881 aacaattagt taagcctgtg aaaataattc cattagaagt agttgttcgt aatattgcta
     2941 gtggatctat tacaaagcgt ttaggttttg aaaatggtga agtttttaga gaaccacttg
     3001 tagaattttt ctataaaaat gatgcgttaa atgatccgtt gataacggat gaccatgtta
     3061 aattgctcaa tatagcatca gatgaagata ttgaaatact aaaatccaaa gcattaaaga
     3121 ttaataatgt gttgaaacaa ttaatggatg ctatgaattt aaaattagta gattttaaaa
     3181 tcgaatttgg aaagactgag actggtcaaa ttttgttagc ggatgaaata tctccagata
     3241 catgtcgaat ttgggataaa gctaccaatg caaactttga taaagatgta tatagaaata
     3301 acactggatc actgattgaa acatatcaaa tatttttaaa caaattggag gatttaaaat
     3361 aatgaaaaca attgaactac atatcacatt acaaccacaa gtattagata cgcaaggaca
     3421 aacgcttact cgagctgtac atgacttagg ttatgcacaa gtgaatgata ttcgtgtagg
     3481 aaaagtatta tatatgacag tggatgaggt tagtgatgaa aaggtacaca acattattac
     3541 aactctaagt gaaaaattgt ttgcaaatac agtgattgaa gaatatagct ataaagtgtt
     3601 agatgatgaa aaggagaatg cataaaatga aatttgcggt tcttgttttt ccaggttcga
     3661 attgtgatag agacatgttt aatgctgcta ttaaaagtgg tgttgaagcg gaatatgtag
     3721 attatagaga aacatcacta agtggatttg atggcgtact tattcctggt ggattttcat
     3781 tcggggatta cttaagatct ggggcaatgg ctagtgtagc gccgattatt tcggaagtta
     3841 aacgtcttgc agctgaaggt aagccagtat taggtgtttg taatgggttt caaattttaa
     3901 ctgaaatagg cttattacct ggtgcattat tgcataacga ttcacattta tttattagta
     3961 gaaatgaaga gttagaaata gtgaataatc aaacggcatt tacaaatctt tatgaacaag
     4021 gtgaaaaagt tatatatcct gtagctcacg gtgaaggtca ttattattgt actgatgaaa
     4081 tatatcaaca attaaaagct aacaatcaaa ttattctgaa atatgtgaat aatccgaacg
     4141 gttcatatga tgatattgca ggaattgtta acgaaaaagg caatgtatgt ggcatgatgc
     4201 cacatcctga aagagcttta gaaacgttgt taggtactga tagtggtgtg aaattatttg
     4261 aagcgatggt aaaaagttgg agggaacaac atgtctaaat ttatcgaacc aagcgttgaa
     4321 gaaattaaac ttgaaaaagt atatcaagat atgggattaa gtgatcaaga atatgaaaaa
     4381 gtttgcgata ttttaggcag acaacctaac tttacagaaa caggtatctt ttctgttatg
     4441 tggagtgaac attgctctta taaacattct aaaccgtttt taaagcaatt tcctacgtca
     4501 ggtgaccatg tgcttatggg gcctggtgaa ggtgcagggg tagtcgatat aggtgataat
     4561 caagccgtag tatttaaagt agagtctcac aatcatccat cagcaattga accatatcaa
     4621 ggggctgcta caggcgttgg tggaatcatt cgtgacattg tctctattgg ggctagacct
     4681 attaatttgt taaacagtct tagatttgga gaattagata ataaacaaaa ccaaagatta
     4741 cttaaaggtg ttgtaaaggg tatcggaggt tatggtaact gcattggtat tccaacaact
     4801 gctggtgaaa tcgaatttga tgaacgttat gatggcaatc cacttgttaa tgcaatgtgt
     4861 gttggtgtta tcaatcacga catgattcaa aaaggcacag caaaaggtgt aggtaattcg
     4921 gtcatttatg ttggtttgaa aactggtcga gatggtattc atggtgctac ttttgcatct
     4981 gaagaattga cggaagaaag cgaaagtaaa cgaccttctg tacaaatcgg tgatccattt
     5041 gtaggtaaaa aattaatgga agcaacactt gaagcaatta catttgatga attagttggt
     5101 attcaagata tgggtgctgc tggtttaaca tcttcatcgt ctgaaatggc ggcaaaaggt
     5161 ggtagtgggt tacatttgag attagaacaa gtgccaacac gtgagccagg tatttctcct
     5221 tatgaaatga tgctttcaga aactcaagaa cgtatgttac tagttgttga aaaaggtact
     5281 gaacaaaaat tcttagattt atttgataag cacgaattgg atagtgctgt tataggtgaa
     5341 gttacagata caaatcgttt tgttttaaca tatgatgacg aagtttatgc tgacattcca
     5401 gttgaaccac tagctgatga agcacctgta tatattttag aaggagaaga aaaagattat
     5461 aatacttcta aaaatgatta tacacacatc gatgttaaag atactttctt taaattactt
     5521 aagcatccga ctatagcatc taaacactat ttatatgatc aatacgacca acaagttggt
     5581 gccaatacga taattaagcc aggacttcaa gcatcggtag tacgtgtgga aggcacaaat
     5641 aaggcaattg cttcaacaat tgatggtgaa gcgcgttatg tatataacaa tccatatgaa
     5701 ggtggaaaga tggtagtagc tgaagcttat cgaaatttaa ttgccgtggg tgcaacacca
     5761 ttagcaatga cagattgttt aaattatggt tctcctgaaa agaaagaaat ctatcaacag
     5821 ttgatagatt caacgaaagg tatggcagaa gcatgcgaca ttcttaagac accagtagtt
     5881 tctggtaatg tatctttata taacgaaacg aaaggtactt ctattttccc aacaccagtt
     5941 gttggaatgg taggtttgat tgaaaatgta aattatttaa atgattttga acctcaagtt
     6001 ggagataaat tatatttaat cggtgatact aaggacgact ttggtggtag tcaacttgaa
     6061 aagttaattt atggcaaagt taatcatgaa tttgagtcat tagatttgag ttcagaagtt
     6121 gaaaaaggtg aatcaatcaa gaccgctatt cgtgaaggac tattatcaca tgttcaaaca
     6181 gttggtaaag gtggcttact gattacctta gctaaactaa gtgcgcatta cggtttagga
     6241 ttaaaatctt caatagatat aacaaatgca caattgttta gtgagacgca aggccgatat
     6301 gttgtttctg ttaaatcagg taaaacttta aatattgata atgcaataga aattggactt
     6361 ttaacagata gtgataattt caaggtaaca acaccatata cagagattag tgaaaatgtt
     6421 tcagatatta aacaaatatg ggaaggggca attgctcaat gtttaactac tcaggattaa
     6481 acgaagaatg tggcgtgttt ggtatttgga atcatcctga agcagcgcaa ctaacatata
     6541 tgggacttca tagtttgcaa catcgtggtc aagaaggtgc aggtatagtt gtttctgatc
     6601 aaaatgaatt aaaaggcgag cgaggattag gcttactaac tgaagcgatt aaagatgatc
     6661 aaatggaacg attaaaagga tatcaacatg caattggtca cgtccgttat gctacttcag
     6721 gtaataaagg tattgaaaat attcaaccgt ttctgtatca cttttatgat atgagtgtag
     6781 gtatttgtca taatggtaac ctcattaatg ctaaatcatt gcgtcagaat ttagaaaaac
     6841 aaggtgctat cttccattcg tcttctgata ctgaagtcat tatgcatttg atacgtcgaa
     6901 gtaaagctcc tacttttgag gaagcgttga aagaaagttt gcgaaaagtt aaaggcggtt
     6961 ttacatttgc gattttaact aaagatgcgt tatatggcgc agtagatcca aatgctatca
     7021 gaccacttgt tgtaggtaaa atgaaagatg ggacatacat ccttgcaagt gaaacatgtg
     7081 caatagatgt gttaggtgca gaatttgttc aagatattca tgcaggtgaa tatgtcgtga
     7141 ttaacgataa aggtattaca gttaaatctt atacacatca tacgacaact gcaatttctg
     7201 cgatggaata tatttatttt gctagaccag actcaacaat agctggtaaa aatgtccatg
     7261 cagtacgtaa agcttctggt aaaaaattag cccaagaaag ccctgtaaat gctgatatgg
     7321 tcatcggtgt acccaattca tcgctatcag ctgcgagtgg ttatgctgaa gaaataggtt
     7381 tgccatatga aatgggacta gttaaaaatc aatatgttgc aagaacattt attcaaccaa
     7441 ctcaagaatt acgtgagcaa ggtgtgagag tgaagttatc tgcggtaaaa gatatagtag
     7501 atgggaaaaa tatcattctt gttgatgatt ccattgttcg cggtacgaca attcgacgca
     7561 ttgtgaaaat gttaaaagat tctggtgcaa ataaagtgca tgtgcgtata gcatcaccgg
     7621 aatttatgtt tccaagtttt tacggaatcg atgtttcaac tacggcagaa ttaatttctg
     7681 caagcaaatc acctgaagaa attaaagatt atattggcgc tgattcatta gcatatctat
     7741 ctgtagatgg gttaattgaa tcaattggtt tagattatga cgcgccatat agtggcttat
     7801 gtgtagaaag tttcactgga gattatcctg cagggttata tgattatgaa gcaaattata
     7861 aagcgcattt aagtcatcga caaaagcaat atatttctaa aaacaaacac ttttttgata
     7921 gcgagggaaa tttaaatgtc taaagcatat gaacaatctg gtgtaaatat tcatgctggt
     7981 tatgaagctg tagaaagaat gtctagtcat gttaaacgta cgatgcgtaa agaagttatc
     8041 ggtggtttag gtggattcgg tgctacattt gatttatcac aattaaatat gacagcgcca
     8101 gttttagttt ctggaacaga cggagtaggt acgaaattaa aactagctat cgactatggt
     8161 aaacatgatt cgataggtat cgatgcagtc gcaatgtgtg ttaatgatat tttaacgaca
     8221 ggtgcagaac cattatattt tttagattac attgctacaa acaaagttgt tcctgaagtt
     8281 attgaacaaa ttgttaaagg tattagtgat gcatgtgttg aaacgaatac tgcacttatc
     8341 ggtggagaga ctgctgaaat gggtgaaatg tatcacgaag gcgaatatga tgtagctgga
     8401 tttgctgttg gagcagttga aaaggatgac tatgtagatg gttcagaagt gaaagaggga
     8461 caagttgtta tagggcttgc gtcaagtggc attcattcaa atggatacag tttagtgcgc
     8521 aaattaatta atgaatcagg cattgatttg gcatcaaact tcgataatcg tccatttatc
     8581 gatgtctttt tagaaccaac taaattatat gtcaaacctg tacttgcttt gaaaaaagaa
     8641 gtttctatta aggcaatgaa tcatattact ggtggaggtt tttatgaaaa tattccacgt
     8701 gcattgccag ccggatatgc tgctagaatt gatactacat catttccaac accaaaaata
     8761 tttgattggt tacaacaaca aggcaatata gacacaaatg aaatgtataa catttttaat
     8821 atgggtattg gctatacggt tatcgttgat gaaaaagatg tatcacgcgc tttgaagatt
     8881 ttagcagaac aaaatgtgga agcctatcaa attggtcata ttgtgaaaaa tgagtcaact
     8941 gcaattgaat tgttgggggt ataacaatgg ttaaaattgc gatttttgca tcaggttcag
     9001 gaagtaactt tgaaaatata gttgagcatg ttgaatcagg aaaacttgaa aatattgaag
     9061 ttacggcgct atatacggat catcaaaatg cgttttgtat agatagagca aaaaagcacg
     9121 atattcctgt ttatattaat gaaccaaaac aatttgattc aaaagcagcg tatgaacaac
     9181 atttagtaac attattaaat gaagataagg tagagtggat tattctagct ggctacatgc
     9241 gtctaattgg tccagactta ttagcttcat ttgaaggtaa aatattgaat atacatccat
     9301 ctctattgcc gaaatataag gggattgacg caataggcca agcatatcat agtggcgata
     9361 ctattactgg ttcgacagta cattatgttg atagtggtat ggatacggga gaaattattg
     9421 aacagagaaa atgtgatatt agaccggacg attcaaaaga acaattagaa gagaaagtaa
     9481 aaaaattgga atatgagtta tatccaagtg ttattgctaa aattgtaaaa taaggagtat
     9541 gtatgtgatg aagaaagcta ttttgagcgt atcaaataaa acaggtattg tagagtttgc
     9601 taaagcgtta acgcaattaa attatgaatt atattcaaca ggtggtacta aacgtatatt
     9661 agatgaagca aatgtaccag ttcgttctgt ttcagactta acacattttc cagaaataat
     9721 ggatggccgt gttaaaacat tacatccggc tgttcatggt ggcattttag ctgatcgaaa
     9781 taaaccgcag catttaaatg aattatcaga acaacatata gatttaattg atatggtagt
     9841 agttaattta tatccattcc aacaaactgt tgcaaaccct gatgtgacga tggacgaagc
     9901 aattgaaaat attgatattg gtggtccaac aatgttacgt gctgcagcta aaaactataa
     9961 acatgtaaca acaattgtac atccggcaga ttatcaagaa gtattgacgc gattaagaaa
    10021 cgattcgtta gatgagtcat atagacaatc attaatgatt aaagtttttg agcatactgc
    10081 agaatatgat gaagcgattg tacgtttctt taaaggggat aaagaaactt taagatatgg
    10141 agaaaatcca caacaatcag cgtattttgt gagaacttcg aatgctaagc acacgattgc
    10201 aggcgctaaa caattacatg ggaaacaatt aagctataac aatattaaag atgcagatgc
    10261 tacactagct ttagttaaaa agtttgatac acctgctaca gttgcggtta aacacatgaa
    10321 tccatgtggt gttggtatcg gtgacacgat agaacaagca tttcaacatg cgtatgaagc
    10381 ggatagtcaa tcaatatttg gtggaattgt tgcattaaac cgagctgtaa cacctgagtt
    10441 agcagagcaa ttgcatagta tctttttgga agtcattatt gcaccaaaat ttacagatga
    10501 agcattagat attttaaaac aaaagaagaa tgtaagatta ttagaaattg atatgactat
    10561 agacagtaac gaagaagagt ttgtttcagt atctggcgga tatttagttc aagataaaga
    10621 caattatgtc gtgccaaaag aagaaatgaa agttgttaca gaagtagcac ctacagatga
    10681 acaatgggaa gcaatgttat taggatggaa agttgtacca tcagtaaaaa gtaatgcaat
    10741 tattttaagt aataataaac aaactgtagg tataggtgct ggacaaatga atcgtgtcgg
    10801 tgctgctaaa attgcgttag agagagctat tgaaatcaat gatcatgtag cgttagtatc
    10861 tgatggattt ttccctatgg gagatacagt tgaacttgca gcacaacatg gtataaaggc
    10921 aattatccaa ccgagtggtt cgattaaaga tcaagattca attgatatgg ctaataaaca
    10981 tggtattgca atggtagtca caggcactcg acattttaaa cactaatttt aaaggggcgt
    11041 aacataaatg aatgtattag taattggtgc tggtggacga gaacatgcac ttgcatataa
    11101 acttaatcaa tcgaatctag ttaaacaagt gtttgtcatt ccaggtaatg aggcaatgac
    11161 acctatagct gaagtacaca ctgaaatttc agaatctgat catcaagcga tactagattt
    11221 tgctaaacgg caaaatgttg attgggtagt tataggtcca gaacagccgc taattgatgg
    11281 attagcagac attttacgag cgaatggttt caaagtgttt ggtccaaata agcaagcagc
    11341 tcaaatcgaa ggctcaaaat tatttgctaa aaagataatg gaaaaatata atattccaac
    11401 tgctgattat aaagaagttg agcgaaaaaa ggatgcttta acatatattg aaaactgtga
    11461 attgcccgtt gttgtcaaga aagatgggtt agctgctggg aaaggcgtta ttattgcaga
    11521 tactattgaa gcagccagaa gtgctattga gattatgtat ggtgatgaag aagaaggtac
    11581 tgttgtattt gaaacgtttt tagaaggtga agagttctcg ctaatgacat ttgttaatgg
    11641 tgatttagca gtacctttcg actgtattgc acaagatcat aaacgcgcat ttgatcatga
    11701 tgaaggacca aatactggtg gtatgggggc ttattgtcca gtaccacata ttagtgacga
    11761 tgttttaaaa cttacaaatg aaacaattgc acaacccatt gcaaaggcaa tgcttaatga
    11821 aggttatcaa ttcttcggtg tattatacat tggtgctatt ttaactaaag atggtccaaa
    11881 agtaatagaa tttaatgccc gttttggtga tcctgaagct caagtattat taagtcgcat
    11941 ggaaagtgat ttaatgcagc atattattga tttagatgaa ggaaaacgta ctgaattcaa
    12001 atggaaaaat gaatctattg taggggtcat gttggcatca aaaggatatc ctgatgcata
    12061 tgaaaaaggg cataaagtaa gtggctttga tttaaatgaa aactattttg ttagtggatt
    12121 aaagaagcaa ggtgatacct ttgttacttc aggtggtaga gttatacttg ccatcggaaa
    12181 aggtgacaat gtacaagatg cacagcgaga cgcatacaaa aaagtatcac aaatacaaag
    12241 tgaccattta ttctatcgtc atgacattgc gaataaagca ctacaactta aataagtaaa
    12301 tttaaaatac taagattagc tatgaacgaa tctataacga tagatttttt catagctttt
    12361 ttagttgtag agtctaggac attgatttct gtaccaaatt tgtgattatg catatgtaat
    12421 acaaaagagg cgccacaaca tgtttggatg aacaaaataa catgtttgtg gcacctcttt
    12481 tgtttagtat ggaataaatg gttttctttt tctatacaat gaatttctaa tttagtatct
    12541 atacaattat ggataaaatt taacctacac gaccaagacg aacatcatct atgcccgtga
    12601 tgggtaaggt gattgaacaa taatatgcca tagtaataat ggcaattaaa actataataa
    12661 agattatatc tttatatgag aaaggtacgt tgtaatagta agtacgagga ccatctctaa
    12721 atcctttcga ctccatcgca actgataatt gatgtgcctt tctaatattt tggcttaata
    12781 gaggtataat taaatgctta aatcgcttta accctctata atttgccgcg tctatcatct
    12841 gatagcgcat ttttaaagat ctgcgaagct gtattaaaga actaatcatt aaaggtatca
    12901 tacgaatggc agccatgaat gcataagcaa cttttgattt aacctttaaa tgttgcatta
    12961 aactataaaa tatcatgaca acctgagatg taagtgcgat taagataccg aaaaatgaaa
    13021 tagcaatggt tcttaatgat acatgtaaac cacgaactaa actttctgtt gtaatatgga
    13081 taaatccgaa tttcaaaatt gtatggctac cattcccgta taaaatcatg aacagggaag
    13141 agagtaatgc aaagccaata cttatagtta taaaaattgc tgtaatttta aactgagtac
    13201 cattaaacat caataagaaa actaacatta agatagtgat ataaagcata aaatcgaaat
    13261 tatgcacaaa tataataaag aaaaatagta taattccaag aaatagtttc gttataatgt
    13321 tgacatcatc aacaaatgat tgccgaactt tccattgctc atacattcgt atcaccatca
    13381 caatctagta acgcaccatc tgaaatttta agtcttcttg atggataacg ttcaattatt
    13441 tcatcgtcat gtgtaaccat gacaatactt tgtcccaaat taattcgctt ttggaaaagt
    13501 ttgatcaact ggaatgtatt atggctatca agtccaaatg tcggttcatc taaaaagata
    13561 atatcagctt tagaacttag tgcggtagct acgctaaggc gtcgtttttg accaatagac
    13621 aactcataag gatgttgatc ttttacattt tgtaaatcta aaagttttaa aagttgtatc
    13681 gtttcatcat cactttgatc tttagaaagg tgattaaaat gaatgttaat ttcatcataa
    13741 accgaatttg ttataaattg taattctggg ttttgataaa ctaggtacat gtgttttgct
    13801 gcatgtttaa tttttgttaa acgctgattt tcaaaataaa catcaccttg atatttaatc
    13861 aattgcataa ttgattcaag caaggttgtt ttaccactac catttgcccc tgtaattgta
    13921 atccactcac ctagaccaat ttctaaatct gagaatgaga gcaatgttga tttaccgcga
    13981 ataatacgtc catttttaaa ttgtaataag tgtgagtttg ttgttggaaa gtcaacacga
    14041 cttggtgcga attcccatgc acgtggatgc cacacaccat attcactgag taaatgaaca
    14101 tacttctgta atatgatttc aggacattca tcggcaatga tatttccgtt ataatccatc
    14161 aaaatgacgc ggtcgacatg attccagatg tgtttaactt tatgttcaac gattacaacc
    14221 gtttgatctt cccaaagttc aattagttta gtccataaat cttctgttgc ttgaacatct
    14281 aacattgctg tcggttcatc taaaaacaat gtttttgatt gttgaagaat ggtttcaaca
    14341 attgccaatt tctgtttcat cccgccactt aaatctttga tatacgtttc aggggtaaca
    14401 tttaaattga ccatatttaa agcattgata attaacgcat ccatgtcttc acgtggtaat
    14461 tgtctatttt ctaaaacgaa tgcaagttct tcgtatactt ttggcataca aaactggcta
    14521 tcagggtctt ggaaaataac gccacttaat gggtcaacga ttagttcatc atatttcata
    14581 ggtaattcaa ttaaattagg aacaatacca cttaatacat tcagaagtgt acttttaccg
    14641 caaccagaag gaccgagtaa aagtactttt tctttgtctt gaatagtgat atttaaatga
    14701 tcgaaaattt tacgttgacc acttggatat tttaatcgta aatcacttac ttttaacact
    14761 ctgaatgctc ctcttataag ttgtcgtaat cttctttagc agctggtcta aataattttg
    14821 ttacgcctgt cttatctaaa gcttttacta aaaggtaaga taggacgccg gcgactactg
    14881 caccactaat taatctaaat acgatgaata atgttaagtt ccaacctgca acttcattta
    14941 aataaccata gaaataatct atcgggaaag ccgcgattgc tgtacaaaaa cctgctaaca
    15001 tagctaccat aactgaacgt gattgatatt taaaaattgc aaagacaagt tcacacgcta
    15061 aaccttgtat aaaagcgtaa acgattgtcg gaatatcgaa acgacccata atgatagttt
    15121 cgccggcacc tgcagcaaat tcagccagta aagcaatacc tggttttgga ataattagat
    15181 agcagacaat cgctgccatg aaccaaaccc cgtttgttaa ttgttcgagg tgaaggcctg
    15241 tagcttgcac accattgtaa acaaaccacc ataaattgta aataactgcg aatactactg
    15301 aaataagtac ggttactagt atttcagata gctttaaacc ttttgacatt tttacatcct
    15361 cctaataaaa aaacgcacaa ccatccatag gaaagttatg cgttcacaat atatattagt
    15421 aaaacatatg tatagtaaca ctttcctacg ctagttcaag ctagatcagg ttcaaagggt
    15481 ttgagggcaa gcctcatctc agtataaaac acccctagtg tgtgcgattt atttaattaa
    15541 ttatactgta agacgtttgt aaacttatgt caataggttg tcttcatgaa atttcgttta
    15601 attcgattta aaatttataa tattagcatt ggatttaaat tgaagatgta gtaggaatgt
    15661 tagtaattaa agatataaaa atatgtgaca tgtaataata ttgagctgat aaatgaagag
    15721 ggatacttat caatcatacc tctttaacaa cagtgaagaa cccgtgcata atggcttacg
    15781 aattatagtt tataaggaag aagagggata catgcgccga gcacatgcat aaaagcccct
    15841 aacaactaaa agttgtaagg aaggagaggg atacatgcgc cgatcacatg cataaaatcc
    15901 cctaacaact aaaagttgta aggggattta aattaattta gtgtatcttg gatatcttgt
    15961 tttgtttgat taatatcttc tgttttttct tcttttttat cttttaattt ttcttcaact
    16021 tctttagctt tttctgctgc ttttttattt tgattttcat tagacatgat taattcctcc
    16081 caaattggat aattatttat atataaatct tacccggttg tactttcgtt aaacttttct
    16141 aagtctatag cactatttat tcatttatct aaagacaaca acattagatt aatatataat
    16201 gattttgagg tgaacataat gtcttttctt aggaaacacg ccgaaattat ttttagctat
    16261 ttaatcggta tcgtttcact cttcactggt ctcattattt taattaactt gccattaatt
    16321 aaacaattaa atggtggtaa aaaagttgat acacatgttc ataatgtgtg ggaatttctg
    16381 aatgcatttt tcagtgaaat tattaaagta atgagtcgat ttataggtga tttccctata
    16441 gttagtgcaa ttgtgataat tatattcggt attttagtta tgttgattgg tcatacatta
    16501 cttagaacta ttaagtatga ctatgatatt tctatctttt tcttagttat cggtatcatg
    16561 tactttatta ttactcttat attaatgact caagtttatg gattctttgc agtgattttc
    16621 attattccat ttacaattca tataggatat atcgtctata aagatgaatt gaatcaggaa
    16681 aatgtaaaaa atcatttcat gtggataatt gtgagttatg gtataagtta cttaattaca
    16741 caaattgcat tgtatggcag aattgatgct aatgaaatag agtcaattga tatcttaagt
    16801 gtcaatgctt tctttataat tatgtggtta cttggtcaaa tggctatttg gaatttcttg
    16861 ttcttgcgcc gagctttacc tttaacaaag caagaattag gtgaagagga gccagaatta
    16921 tcaagaacaa gtaaagggaa tgtcacgaat caaactaaaa ttcacttgaa acaactccaa
    16981 gataagacta cagaatatgc acgtaagaca agaagaagtg tcgatttaga taaaattaga
    17041 gctaaaagag ataaattcaa aaagaaagtt aatgatatta tcgatattca agaagacgat
    17101 attcctgatt ggatgagaaa accgaaatgg gttaaaccaa tgtatgtcga actattttgt
    17161 ggtgtcgtca tctttttatt cacattttta gaatttaata atcgtaatgc attatttgta
    17221 tctggtgatt ggaaattatc acagacacaa tatgttattg aatgggttac attattaatt
    17281 ctgttattca ttattatcgc atatatcgct acaacgttaa ctttccactt gaaaggtaag
    17341 ttttattatt tacaattatt tatggggagc attttattct ttaaattgtt aacggaattt
    17401 ataaatataa tgattcatgg actattactt tcagtgttca ttacgccaac attactatta
    17461 atgttattgg caatcatcat ttcttattcg ttacaattac gagagcgacc ataattaaaa
    17521 gcattataaa agtactatct attaaacatt ttgatgtgta cgctataagt tagatatatc
    17581 tctaacttac ttagatacag gtcaatgaag tttatggata gtactttttt tgtaactaga
    17641 tttgattgat tcaggtgatg tgaattaagt attgaaaatt gtatacaaag ttttagtgca
    17701 aataaaatag ttgaaaagtt atccatttgt aaaatcaaga aaactagtaa atagttgaag
    17761 cgacttatgg aatttgcgaa acgatatata gtatttcctt tgtagaaatt taacatatat
    17821 cattcgaatt actaatttgt taaaatcaac agtaagatta gaagtagatg atattgaaat
    17881 ttggcaaaca atttaatcta tataaaacta caacgaaaca cagaaaggaa gttgtcagat
    17941 gaaaatagca actctgaaca aaggcaaaga aacaaaatat tttaatggat atcctttaat
    18001 tgaagaagag gatatctatt cacaagatca tttaaaagaa ggagatattt ttcaaattgt
    18061 gactgataaa tcacaatatg tcgcaacggc ttatgttggt cgccaacata aaggattagg
    18121 ttgggttcta acatacgata aagctcaaca aatcaacaca gctttctttg taaaattgtt
    18181 taatactgca ttagcagaac gtgattatta ttttaatata gatggaacaa atgcttttag
    18241 attatttaat gctgaaggtg atggtgttgg gggattaaca atcgacaatt acgatggtca
    18301 tttgttgatt caatggtact caaaaggtat ttataaattt aaatatgcca ttcttgaagc
    18361 ggttagaaaa gtatttgatt ataaatctat ttacgaaaaa gtaagattta aagacagcga
    18421 atatagtggt ggttttgttg aaggagatgc acctgagttt ccaattgtta tcgaagaaaa
    18481 cttcacattt tataatgtag accttgaaga tggtttgatg acaggtatct ttttagatca
    18541 aaaagaagtg cgcaagaaat taagggatca atatgccaaa gaacgccatg ttttaaactt
    18601 atttagttat acaggtgctt tttctgcaat agcagcaagt gaggcatctt caacaacaag
    18661 tgtagatttg gctaatcgtt ctcgtagttt aactgaagaa aattttggat taaatgctat
    18721 tgatcctaaa tcccaatata tttatgtcat ggacactttt gatttctata aatatgctgc
    18781 acgacatgga catagttatg acacgatcgt gattgatcca cctagctttg cgcgtaacaa
    18841 aaaacgtaca ttttcagtgc aaaaagatta tgacaaatta attaatggcg ccttaaatat
    18901 cttatcatct gaaggaacat tattgttatg tacaaacgca agtgtatatc cattaaagca
    18961 atttaaaaat actattaaaa agacgcttga agagagtggc gttgattatg aattaactga
    19021 agttatggga ttaccaaaag attttaaaac gcatccacat tataagccat ctaaatattt
    19081 aaaagctgtt tttgtaaata ttagacatta atttgataaa aaggggtata gtaacttatt
    19141 gagaaaaaga agggtgataa tattatggga ttcaaaaaca atttaacatc aaatttaaca
    19201 aataaaatcg gtaattcagt ctttaaaata gaaaatgttg acggaaaagg tgcaatgcca
    19261 acgacgattc aagaattgag agaaagacga caacgtgctg aagcaattgt aaagagaaag
    19321 tctttaatgt catcaacaat gagcgttgtt ccaattccgg gtttagattt tggtgttgat
    19381 ttaaaattaa tgaaagatat tatcgaagat gttaataaaa tttatggttt agatcataag
    19441 caagttaata gccttgggga tgatgtgaaa gaaagaatta tgtctgcagc agcaattcaa
    19501 ggtagtcaat ttattggtaa aagaatttca aatgcatttt taaaaattgt aattagagat
    19561 gtagctaaac gtactgctgc aaaacaaaca aaatggtttc ctgttgtagg acaagctgtg
    19621 tctgcatcta ttagttacta ttttatgaat aaaattggaa aagatcacat tcaaaaatgc
    19681 gaaaatgtta ttaaaaatgt catgtaggtg ctataatagt tttgcaattt gcaaatttta
    19741 ctgaaaccgg ttttaaacga attgaattta aagcatggtt ttggtaaagt taatgtataa
    19801 aactaagtta gtattgtaat aatattgaag attctaacta tacgaaggag aaatgtaatt
    19861 atggaacaaa attcatatgt aatcatcgac gagactggta ttcacgctag accagcaaca
    19921 atgttagtac aaacagcttc aaaattcgat tctgatattc aattagaata taacggtaag
    19981 aaagtaaact taaaatcaat catgggtgtt atgagccttg gtgttggtaa agatgctgaa
    20041 attacaattt atgctgacgg tagtgatgaa tctgacgcca ttcaagcaat cagtgacgtc
    20101 ttatcaaaag aaggattgac taaataatca tgtctaaatt aattaaaggt attgccgcat
    20161 ctgatggtgt cgcaattgct aaagcttatt tattagttga gccagactta acattcgaca
    20221 aaaatgaaaa agtcactgat gttgaaggag aagttgcaaa gttcaatagc gctatcgaag
    20281 cttctaaagt tgagttaact aaaattagaa ataatgcaga ggttcaacta ggtgctgata
    20341 aagctgctat ctttgatgca catttattag ttttagatga ccctgaatta attcaaccaa
    20401 tccaagataa gattaaaaat gaaaacgcta atgctgctac agcattaacg gatgtaacaa
    20461 cacaatttgt tacaattttt gaatctatgg ataacgaata catgaaagaa cgtgcggctg
    20521 atattcgcga cgtttctaaa cgtgtgttat cacatatttt aggtgtagaa ttaccgaatc
    20581 cgagtatgat tgatgaaagc gttgttattg tagggaatga cttaacgcca tctgatactg
    20641 ctcaattaaa taaagaattc gtacaaggtt ttgctacaaa cattggcgga agaacaagtc
    20701 actctgcaat tatgagtcgt tctttagaaa ttccagcaat tgttggtaca aaatcaatta
    20761 ctcaagaagt taaacaaggc gacatgatta tcgtagatgg attaaatggt gatgtaatcg
    20821 ttaatccaac tgaagatgag ttaatcgctt atcaagataa acgtgagcgt tattttgctg
    20881 acaagaaaga attacaaaaa ctacgtgatg ctgatactgt tacagttgat ggtgttcacg
    20941 cagagcttgc tgcaaatatt ggtacaccta atgatttgcc aggtgttatt gaaaatggtg
    21001 cacaaggtat cggcttatat agaactgagt ttttatatat gggtcgtgac caaatgccta
    21061 cagaagaaga acaatttgaa gcttataaag aagtattaga agcaatgggc ggtaaacgtg
    21121 ttgttgtacg tactttagat ataggtggag ataaagaatt atcatactta aacttgcctg
    21181 aagaaatgaa tccattctta ggttaccgtg cgattcgttt atgccttgcg caacaagata
    21241 ttttcagacc acagctacgt gcattattac gtgcatcagt ttatggtaag ttaaatatca
    21301 tgttcccaat ggttgcaaca attaacgaat ttagagaagc taaagctata ttattagaag
    21361 aaaaagaaaa ccttaaaaat gaaggtcatg acatttcgga tgatatagaa ttaggaatca
    21421 tggtagagat acctgcaaca gcagcattag ctgatgtctt tgctaaagaa gtagatttct
    21481 tcagtatcgg tacaaatgat ttaattcaat acacattagc tgctgaccgt atgtcagagc
    21541 gtgtatcata tctataccaa ccatataacc cttcaatctt acgtttagtt aaacaagtta
    21601 ttgaagcgtc acataaagaa ggtaaatgga caggtatgtg tggtgaaatg gctggagatg
    21661 aaacagctat tccattattg cttggtttag gtttagatga gttctctatg agtgcaacgt
    21721 ctattctgaa agcaagaaga caaattaatg gtttaagtaa aaatgaaatg actgaacttg
    21781 ctaaccgtgc agtcgactgt gcaacgcaag aagaagttat tgaattagtt aacaactacg
    21841 taaaataatt aattctttcg aaagacagaa ttataaataa acccgaagta ttcattgtaa
    21901 gtaattatga atacttcggg ttctttttta tgatttatgt taatttcaat ggaatgttgt
    21961 tgctaactaa ttaaacaact atgtgtcatt aacaaaatgt agttctttaa ttaatgttag
    22021 ttaatttaaa aatcgatatt taatttatga tagctataaa tgatgaagtt gatttagatg
    22081 aaaattaatc ttggatattt aatactttgt tgatttcatc aagatcaaca tggtacattg
    22141 gattgccatt caacaaaata aacggagttg aaaaagcatc aaaatctatc atttcgtttc
    22201 gatattgttg attgttgata tttctctctt caaaatcaat gtgatgctca tttagataat
    22261 tttttacaaa tgtacaaggt ggacaatcat tctgcgtata aacgattatt tctgacatgt
    22321 atcatgctcc ttcgtgcttt attctaatgt aatatacaca attatacatt gaaattcaaa
    22381 tttgtgaaca cattgtgaac tgacataaat tatacacaat ttcatagcgc ataatgtttt
    22441 aaaaatacac attatagcaa ttataattaa gttcattgtg ttgtcatcaa aaaaagaaaa
    22501 aggtgatgtt tttaaatgga tacagttgaa atcagtcggt ttttgacagc tatgacttta
    22561 gcagttcata tcatttttgc aacgattggt gttggtatgc ctttaatgtt cgcaattgca
    22621 gaatttttag gtattcgcaa aaatgatctt caatatatag ctatggccaa aagatgggct
    22681 aaagcttata caattactgt agcagtggga gttgttacag gtacaattat aggacttcaa
    22741 ttatcattga tttggcctac atttatggaa atgggtggac acgttattgc acttccatta
    22801 tttatggaaa catttgcgtt cttctttgaa gctattttct taagtatata tttatatact
    22861 tgggatcgtt ttaaaaataa atggacacat ttcttaatta gtataccagt aattattggt
    22921 ggctctttct cagcattctt cattacttca gtgaattcat ttatgaatac gcctgcaggt
    22981 tttgagttga agaatggaaa gatggtcaat gttcaaccta tagaagcgat gtttaaccca
    23041 tcgtttatag ttcgatcatt tcacgtaatt actacagccg gtatgacgat ggcatttgtt
    23101 attgcatcaa tagcagcttt taaattattg cgtaatcgtc aacctaaaga tactgtctac
    23161 cataagaaag ctttgaaaat gtctatgata gttggattct tttcaacatt actttctatg
    23221 ttggcagggg atttatctgc aaaatttttg cataaattcc aacctgaaaa attagcagct
    23281 tatgaatggc atttcgatac atcttcccat gccaaattat tattatttgg tgtgttagat
    23341 gaaaagactc agcaagttaa aggtgcgatt gaattacctg gactattgag tttcttagca
    23401 gataatagtg tcaaaactaa agtacaaggg ttaaatgatt ttccaaaaag tttacatccg
    23461 cctatgatcg tccattattt ctttgattta atggtaacga tgggaatttt atgttttgtc
    23521 atttcaggtg tttatgtctt aactttaatg tttaaaaagc ttagaaagtt ttctactcat
    23581 aaatggatgc tttatggaat attattaaca ggcccagctt caatgctagc tatagaattt
    23641 ggatggttct taacagagat gggtagacag ccttggattg ttcgtggtta tatgcgcgtg
    23701 gcagaagcag caacacaagc aggcggaata accttcgtta caattttatt tggcatattg
    23761 tacatcattt taatgtatac atgtgcatac gtattaattc gtatgtttaa aaataaaccg
    23821 gcgtatgaag atgtaaatcg tttagccaag aagcaaggag gagaaataga aaaatgattt
    23881 atgcatttat aggtataaca gtgttatggt tgtttttatt ttgctatatc attattgctt
    23941 ctatagattt tggggcaggt ttcttcgcat tgcattcaaa gttaactggt gatgaaaaga
    24001 aaattaatca cttaatttca cgttatttaa acccagtttg ggaagttacg aatgtattct
    24061 ttgttttctt cttcgtagga ttcgtaggtt tctttccaga gtcaatcaaa tatttaggca
    24121 cggtattgtt aataccaggt tcaatagcac tgattatgat atcgttgaga aacagttttt
    24181 atgcatttga aaattatggt caagatacaa aattagcatg gatgatcatg tatggggtaa
    24241 gtggattatt aattccagct tcattatcta ctgctttaac tattacagaa ggtggctata
    24301 ttaatgttcg aaacaatgtt atcgatctag attgggtgca gttattatta agcccatttg
    24361 cttggtctgt agtattcttg gcaattattt cagttttata tatttcatca ggatttttga
    24421 catattatgc taaaaaagca aatgacgaac cagcatataa tttaacaaga caatggcaca
    24481 tatttttagg gccgccgatg attatcattt gtttattcgt atttctatca ttacgcattc
    24541 aaaattctga acatttttat tcagctgttt ttgactattg gtggatgttt ggtataagtt
    24601 tcttattctt tgcattagct tcattgttaa cattctttaa gaaaaaacat ggtttggctt
    24661 ttgtatttgt tattttacaa atgatgttcg cgttctttgg ctatggaatt agtaaattgc
    24721 catacctttt atatccgttt gtaaaaatta cagatgcata tgttaatcca gaaatgggct
    24781 ggacattagt gattgtcttt attttaggtt tacttttatt acttccatcg ttaatattat
    24841 tattaagatt atttgttttc gacaaagaat atgttgaagg aaagaaatca taagttgaga
    24901 cgatacccca actttcattt acttgcatta ctgcatttag ataatttagt tggggtactc
    24961 atttataata attttaaatt tatattttca atgaattgat gaaaatcatt taatggaggg
    25021 cgagtatggg taaagaatat gtagtcatcg gtctaggtcg ttttggaggt agtatcgttc
    25081 gtgagttgaa tgcattagac atggatgtaa tggccatcga ccatgatgaa aatagagtga
    25141 atgaatatag tgatatcgct actcatgcgg ttgttgcaga cactacagat gaagcagtta
    25201 tgaaaagttt aggtatccgt aactttgatc atgtcattgt ggcaattggt gaaaatattc
    25261 aatcaagtac gttgacgact ttaattttaa aagagttagg tgtaaaaaaa gtaactgcta
    25321 aagcacaaaa tgattatcat gcaaagattt taaataaaat tggagcagat acggttgtgc
    25381 accctgagcg tgatatgggt agacgtattg cgcataatgt tgcgagtgca agtgtacttg
    25441 attatcttga gttggcagac gagcattcta ttgtagaatt gaaagcaact gaaaagatgg
    25501 cggggcagtc tatcattgat ttagatataa gagcacaata tggaattaac attattgcaa
    25561 ttaaacgagg caaagagttc atcatttcac caaatccaaa tattaattta gaaataggtg
    25621 atattttaat catgattgga catgataatg atttaaatcg ctttgaaaaa aatattgcga
    25681 cgagataatc gggagtggga cagaaatgat attttctcaa aatttatttc gtcgtcccac
    25741 cccaacttac attgtctgta gaaattggga atccaattcc tct
//
LOCUS       DANRDI010000022        25442 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.022, whole genome shotgun sequence.
ACCESSION   DANRDI010000022 DANRDI010000000
VERSION     DANRDI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.022"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1004
                     /locus_tag="PZ377_000707"
     CDS             <1..1004
                     /locus_tag="PZ377_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDM6357595.1"
                     /translation="ASHIEDLSFGYNNGYGYIKLRDIIVERMSKQGINVGRENVMITS
                     GALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHIDVPYNQINEIDTIIDR
                     FINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPIIEDDIFRDIFFSDPTP
                     SIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLADVRMQIDYGSSILSQMV
                     VYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHWEVPSGGYFVWLVFKID
                     IDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNENIKHALYKIYTYVKKV"
     gene            1194..1667
                     /locus_tag="PZ377_000708"
     CDS             1194..1667
                     /locus_tag="PZ377_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDM6357596.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            1929..3521
                     /locus_tag="PZ377_000709"
     CDS             1929..3521
                     /locus_tag="PZ377_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDM6357597.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(3850..5352)
                     /gene="spa"
                     /locus_tag="PZ377_000710"
     CDS             complement(3850..5352)
                     /gene="spa"
                     /locus_tag="PZ377_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDM6357598.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGKEDGNKPGK
                     EDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVD
                     KKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL"
     gene            complement(5773..6525)
                     /gene="sarS"
                     /locus_tag="PZ377_000711"
     CDS             complement(5773..6525)
                     /gene="sarS"
                     /locus_tag="PZ377_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDM6357599.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
     gene            complement(6894..7892)
                     /gene="sirC"
                     /locus_tag="PZ377_000712"
     CDS             complement(6894..7892)
                     /gene="sirC"
                     /locus_tag="PZ377_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDM6357600.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(7889..8884)
                     /gene="sirB"
                     /locus_tag="PZ377_000713"
     CDS             complement(7889..8884)
                     /gene="sirB"
                     /locus_tag="PZ377_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDM6357601.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(8900..9892)
                     /gene="sirA"
                     /locus_tag="PZ377_000714"
     CDS             complement(8900..9892)
                     /gene="sirA"
                     /locus_tag="PZ377_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDM6357602.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            10123..11103
                     /gene="sbnA"
                     /locus_tag="PZ377_000715"
     CDS             10123..11103
                     /gene="sbnA"
                     /locus_tag="PZ377_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDM6357603.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            11100..12110
                     /gene="sbnB"
                     /locus_tag="PZ377_000716"
     CDS             11100..12110
                     /gene="sbnB"
                     /locus_tag="PZ377_000716"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDM6357604.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            12131..13885
                     /gene="sbnC"
                     /locus_tag="PZ377_000717"
     CDS             12131..13885
                     /gene="sbnC"
                     /locus_tag="PZ377_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDM6357605.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            13878..15134
                     /gene="sbnD"
                     /locus_tag="PZ377_000718"
     CDS             13878..15134
                     /gene="sbnD"
                     /locus_tag="PZ377_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HDM6357606.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            15124..16860
                     /gene="sbnE"
                     /locus_tag="PZ377_000719"
     CDS             15124..16860
                     /gene="sbnE"
                     /locus_tag="PZ377_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDM6357607.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            16769..18619
                     /gene="sbnF"
                     /locus_tag="PZ377_000720"
     CDS             16769..18619
                     /gene="sbnF"
                     /locus_tag="PZ377_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDM6357608.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            18594..19370
                     /gene="sbnG"
                     /locus_tag="PZ377_000721"
     CDS             18594..19370
                     /gene="sbnG"
                     /locus_tag="PZ377_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDM6357609.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            19370..20572
                     /gene="sbnH"
                     /locus_tag="PZ377_000722"
     CDS             19370..20572
                     /gene="sbnH"
                     /locus_tag="PZ377_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDM6357610.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            20576..21340
                     /gene="sbnI"
                     /locus_tag="PZ377_000723"
     CDS             20576..21340
                     /gene="sbnI"
                     /locus_tag="PZ377_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDM6357611.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            21536..21943
                     /locus_tag="PZ377_000724"
     CDS             21536..21943
                     /locus_tag="PZ377_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357612.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            21993..22619
                     /locus_tag="PZ377_000725"
     CDS             21993..22619
                     /locus_tag="PZ377_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6357613.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            22831..23607
                     /locus_tag="PZ377_000726"
     CDS             22831..23607
                     /locus_tag="PZ377_000726"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDM6357614.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            23751..23873
                     /locus_tag="PZ377_000727"
     CDS             23751..23873
                     /locus_tag="PZ377_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357615.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            23943..24923
                     /locus_tag="PZ377_000728"
     CDS             23943..24923
                     /locus_tag="PZ377_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDM6357616.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            24886..>25442
                     /locus_tag="PZ377_000729"
     CDS             24886..>25442
                     /locus_tag="PZ377_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDM6357617.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQI"
BASE COUNT         8420 a         3895 c         4886 g         8241 t
ORIGIN      
        1 cagccagtca tattgaagac ttatcttttg gttataataa tggctatggt tatatcaagt
       61 taagagatat tatcgttgaa cgaatgtcaa agcaaggtat aaatgtaggt agagaaaatg
      121 taatgatcac ttcaggcgct ttacatgcca ttcaactttt atctattggg tttttaggtc
      181 aagatgccat aataatttcg aatacaccat catatattca ctctacaaat gtttttgagc
      241 aattgaattt tagacatatt gatgttcctt ataatcaaat taatgaaatt gataccatca
      301 ttgatagatt tattaatttt aaaaataaag cgatttatat agaacctagg tttaataacc
      361 cgacaggtcg ttctttaacg aatgagcaaa agaaaaatat aattacttat agcgaaagac
      421 ataatattcc tatcattgaa gatgatatct ttagagatat tttctttagc gatccaactc
      481 cttctatcaa aacttatgat aaattgggaa aagttataca tataagcagt ttttcaaaaa
      541 cgattgcacc agcaataaga ataggttgga ttgttgcttc tgaaaaaata atagagcaat
      601 tggcagatgt aagaatgcaa attgactatg gatccagtat tttgtcacaa atggttgtat
      661 atgagatgtt gaaaaataag tcttatgata aacacttagt aaagttaagg tatgttttaa
      721 aagataaacg agactttatg ttaaacatcc tcaataattt atttaaggat atagcacatt
      781 gggaggttcc aagtggaggt tattttgtat ggttagtctt taaaatagat atagatatta
      841 aatatttatt ttacgaattg ttaagtaaag aaaaaatatt aatcaatccg ggttacattt
      901 atggcagtaa agaaaagagt ataaggctat cttttgcctt tgaatcaaat gaaaatatta
      961 agcatgcgct ctataaaatt tatacatatg tgaaaaaggt ttaattaaaa caataattcg
     1021 aatcattatg tggcatgtta aacagctaaa tataagctat gcacatttaa caagcggata
     1081 ctcctgacgc aattatagta tcattacata ttgtttaatc gtttggggta atgtaattaa
     1141 aaggtaaaat aggcagagtg ttatacataa aatgtaaaga aagaggcgat agaatgtcta
     1201 aaatcaggtc ttttacaata ttaagtctac ttatttactt agctatgatg tgctatacag
     1261 tagtgaccta tccaaaatta ccaaccaaag tgcctattca ttataattta gcaggggatg
     1321 ctgataattt tgctgataaa tgggtgctgc ttttgattaa tagcgcattt atagtgattt
     1381 ggcttatatt tttcattgca ggtagatact atgaacgatt tgccaaatgg tcacattata
     1441 atcatacacc acgtgaaatt cgagcgatta aattattttt aagtacgtta aatttagaga
     1501 ttatgagcta tatgtctatc ttcacagtat tagaaatttg gcaaatacaa caccatcatc
     1561 aattcaattt actatggttt aatatgatat ttatcattat cattggtttg acgcttgtca
     1621 tattttgttt acttcctaca attcataaaa tgagagattc tcaataaaaa tataaactct
     1681 catgatgcag ttttgaagcg aattcctgag attattataa ataatatcta gtatttataa
     1741 ttaaaatgat atattattta ttatagttaa atattgtgta tattttgtga actttttgta
     1801 aaaattcgat tgcctgtcac atataggagt gttacatttt aaatatgtga tcatcgcaaa
     1861 atataagttg aaataggttg tagattaatc agaatgataa atattttata taaagagagg
     1921 gagtcattat gacactactt actgtaaatc cattcgataa tgtcggatta tcagccttag
     1981 ttgcagcagt acctattatt ttatttttat tatgcttaac cgtttttaaa atgaaaggca
     2041 tttatgcagc attgacaact ttggttgtta cattgattgt ggctttattt gtatttgaat
     2101 taccagcgcg tgtatcagca ggtgcgatta cagaaggcgt tgttgccggt attttcccaa
     2161 taggatatat cgttttaatg gcagtttggt tatataaagt ttctattaaa acaggacaat
     2221 tttctattat tcaagatagt attgcaagta tttcagtgga ccaaagaatc caactattat
     2281 taattggatt ttgtttcaac gcatttttag aaggtgcagc aggatttggt gtgccaattg
     2341 cgatttgtgc agtattatta attcaacttg gatttgaacc attaaaagca gcgatgttat
     2401 gtttaattgc taatggtgcg gcgggtgcct ttggtgcaat tggtttacca gttagtatta
     2461 ttgatacgtt taacttaagt ggaggcgtta caacattaga tgttgcgaga tactcagcat
     2521 taacacttcc aattttaaac tttattattc catttgtttt agtattcatt gtagatggta
     2581 tgaaaggtat taaagaaatt ttacctgtca ttttaacagt gagtggtaca tatactggat
     2641 tacaattatt attaacaata ttccatggtc cagaactagc agacattatt ccatcactag
     2701 caacaatggt ggtgttagca tttgtttgtc gtaaatttaa accgaaaaac attttcagat
     2761 tggaagcgtc tgaacataaa attcaaaaac gaacgcctaa agaaattgtc tttgcttgga
     2821 gtccgttcgt aattttaact gcctttgtat tagtatggag tgcaccattc ttcaaaaaat
     2881 tattccaacc tggaggtgca cttgaaagtt tagtaataaa attgccaatt ccaaatactg
     2941 tgagtgattt atcgcctaaa ggaattgcgt tgcgtctcga tttaattggt gcaactggga
     3001 cagcgatttt attaacagta attattacaa ttttaattac gaagttaaaa tggaaaagtg
     3061 caggtgcttt attggtcgaa gcaattaaag aattatggtt accgatcctt acaatttcag
     3121 ctatcctagc tattgctaaa gttatgacat acggtggttt gactgtagca attggacaag
     3181 gtattgctaa agcgggagca attttcccat tattctctcc agtattaggt tggattggtg
     3241 tgtttatgac tggttcagtt gtaaataaca atactttatt cgcacctatt caagcgacag
     3301 tggcacaaca aatttcaaca agcggttcat tacttgtggc agctaacact gcaggtggtg
     3361 tagcagcgaa acttatttca ccacaatcaa ttgccattgc gactgcagct gttaaaaaag
     3421 ttggtgaaga atctgcatta ttaaaaatga cgttaaaata cagtattata tttgttgctt
     3481 ttatttgtgt ttggacgttt atactaacgt taatattcta aatataaata atgttgtcac
     3541 ttggattcaa atgacatttt aaatctaatt attcatgaat cgaactagta cgaaatgcaa
     3601 tgagcatctt gtctagttcg attttttaat gcctaaaaat gtcatatatg taatcagagt
     3661 agaaagtgtt gaggcgtttc agaagttgtt tagaaaagta agtaaaataa aaaatgcact
     3721 gagcaacaaa agatgttgct cgtgcattta gatgattctt atcatttcaa ataagaatgt
     3781 gttaatcaac gtatataagt taaaattggt ttggataaaa tgatatctat cgttgtgtat
     3841 tgtttgtttt tatagttcgc gacgacgtcc agctaataac gctgcaccta aggctaatga
     3901 taatccacca aatacagttg taccgatgaa tggattttct tcaccagttt ctggtaatgc
     3961 ttgagctttg ttagcatctg catggtttgc tggttgcttc ttatcaacaa caagttcttg
     4021 accaggtttg atcatgtttt tatcagctaa tttgttatct gcagcaattt tgtcagcagt
     4081 agtgccgttt gcttttgcaa tgtcatttac tgtatcacca ggtttaacga catgtactcc
     4141 gttaccatct tctttaccag gtttgttgcc atcttctttg ccaggcttgt tgccgtcttc
     4201 tttaccaggt tttttgttgt cttctttacc aggcttgttg ccatcttctt taccaggttt
     4261 tttgttgtct tctttaccag gcttgttgcc gtcttctttg ccaggcttgt tgttgtcttc
     4321 tttgccaggc ttgttattgt cttctttgcc aggcttgtta ttgtcttcct cttttggtgc
     4381 ttgagcatcg tttagctttt tagcttctgc taaaatttct ttgctcactg aaggatcgtc
     4441 tttaaggctt tggatgaagc cgttacgttg ttcttcagtt aagttaggta aatgtaaaat
     4501 ttcatagaaa gcattttgtt gttctttgtt gaatttgttg tcagcttttg gtgcttgagc
     4561 atcatttagc tttttagctt ctgctaaaag gttagcgctt tggcttgggt catcttttag
     4621 gctttggatg aaaccattgc gttgttcttc gtttaagtta ggtaaatgta agatttcata
     4681 gaaagcattt tgttgttctt tgttgaattt gttatccgct ttcggtgctt gagattcatt
     4741 taacttttta gcttctgaca ataggttagc actttggctt gggtcatctt ttaagctttg
     4801 gatgaaacca ttgcgttgtt cttcgtttaa gttaggcata ttcaagattt catagaaagc
     4861 attttgttgt tctttgttga aattgttatc agctttcggt gcttgagatt cgtttaattt
     4921 tttagcttca cctaaaacgt tagtgctttg gcttgggtcg tctttaagac tttgaatgaa
     4981 gccgttacgt tgcgcttcgt ttaagttagg catgttcaag atttcataga aggcgctttg
     5041 ttgatctttg ttgaagttat tttgttgcgc atcagctttt ggagcttgag agtcattaag
     5101 tttttgagct tcacctaaaa cgttagcact ttggcttgga tcatctttaa ggctttggat
     5161 aaaaccattg cgttgatcag catttaagtt aggcatattt aagacttgat aaaaagcatt
     5221 ttgttgagct tcatcgtgtt gcgcagcatt tgcagcaggt gttacgccac cagatataag
     5281 taatgtacct aaagttacag atgcaatacc tacacctagt ttacgaattg aataaatgtt
     5341 tttctttttc aaattaatac cccctgtatg tatttgtaaa gtcatcataa tataacgaat
     5401 tatgtattgc aatactaaaa tctatattta taattaaatt taaaggtaag ttttacaact
     5461 tataaaataa atattttgct gaaagattta ttcaggaagt aaaaggctta aaaccgcaaa
     5521 atcacgctat ttcgattaat aaaaacagat aaaatataga gatatttttt ataatgaaag
     5581 cggtttaata catatgagtt aaaaaatatt tttaagaata aagtggggct ttgaatgtgc
     5641 tgaggtttaa tttcggaaat ggttttaagt gatatagaaa atagaaagtt gttcattatg
     5701 tgctgaggtt ttattaaaaa atagaaaaca caagtgcact ttagaataca gcacacttgc
     5761 gtaatagaga tattattcaa aaacaagatg taaatgatct ttatctgcta ataattgatt
     5821 cacttgagct aataattgtt cagcatggtc ttgctgcgcg tcatccatat gaattaaaat
     5881 ttttctttca tcttcagttg agcgttcttt tattagatag ccttgctttt ttaaattatt
     5941 gagagctcta acagtttgag ggtatttatg gtggattgtt tcaattaaat ctttaagaag
     6001 aacgatgttt ttattttgag aagtgataat agctagaatt gtgaattcta caaaacttaa
     6061 tgttagatgt tttttgataa tattcttgaa atacattgta tacatcatca agtttagaaa
     6121 ttctttacta tcttttggta tcatctgtga ttcactttga tctgcaaggt taaattgttt
     6181 aatgatttga tcaaacaatg taacacgttc tgcaattttc tctcgttgtt cttcagatat
     6241 tgaaatgtaa gtattacgct catcaatttt acttcgaact ttactaatat atgaatgttt
     6301 cacaagtact tttatatgct gtactaaatc cgattgttta taacataaat ctgaaacaat
     6361 cttcttaaat ggaagtgtgt tttcttgttg atgaaataaa taagtcagta atataaattc
     6421 ttttatagtc atatcgactt caggcttgac ttttttctta aaacgaaaca tatatgcttc
     6481 aatgattata aaatctctaa ttttgtcatg gttattatat ttcattgttt tatctccttg
     6541 tatatgcact ttattttaat ataacacaat ataataagaa aaataatcca taatttacaa
     6601 ttaaaaataa tatgatattt ttattaaaat ttgtgttttt gaataaatga cattatcaaa
     6661 cactatattt aaataagaaa tagttatcga tatttgaaaa atcgaagaat attgattgat
     6721 attatagaat atgagtcggt gttatttgta tataaagtat taatatgtaa ttaagaaagt
     6781 cgacgattgt taacaataac aacaactata tgtataaaaa aacctcgcaa cggttagtta
     6841 actgaatcat ttagctaact tcgttgtgag gtcattttgt tttaataata tcgttataac
     6901 tttttcacgg ttaataataa gtatatgaag aatggggcac caaaagcagc aataaataca
     6961 cctgctggca cttctttagg caagaataag gtacgcccaa ttaagtctgc aataacaatt
     7021 gatatggcac caatcattgc tgacattagt aactttttag cataacttcc gcgaacgatt
     7081 gttttcgcga tatgtggtgc gattaaaccg acaaacccaa tgttacctac taaactgatt
     7141 gccatagata cgagtatagt agaagtgatt aattggatta gtttcatacg ttgtacatgt
     7201 aagcctaagc caatcgctac agggtcatca agtatagata ttttcatttt tggtataaca
     7261 agaaataaca acggcacaac agctaaaata accataccca aaatgattgt atctttaaac
     7321 gtagcaccgt aaagacttcc gactagccat gtataagctt tggcagcaga taattgcttc
     7381 gttgtaatga gtaatccttg gacaagcgca ataaacaacg tttgcatcga aataccgatg
     7441 attatgagtg ttgtcgggcg tatttgtcct ttcgtttgaa acactaatag tatcatcatt
     7501 gcaactgcgc cacctaatac tgcaaatagt ggaagtaaat gtattgttaa atggctgaaa
     7561 aatgcaataa agacaacagc acttaagcta gcaccacctg tgataccgat aatatcaggt
     7621 gaggcaattg gattttttaa tacattttgc aacattaaac cactcattcc tagtgcggca
     7681 cctgctaaaa tcgcaagtgt aatgcgaggt aagcgtaata cttctaaagt gaattgatcc
     7741 atactgtcat ttggatttat aaagtacatc agtacgcgtt gtaatggtat aaagcttgaa
     7801 ccaatcatca tacttaccac tgaaacgatg gctaaaaaga ttaacgcgaa gatgagatgg
     7861 taattgtctt ttttattaat cttttcggtc ataagcgttg acgtcctttc ttcataatat
     7921 agattaagac aatagcgcca atgacagcgg taacgacacc gataggcaac tctagtggct
     7981 taattattat acgagcaaca atgtctgaaa tgatcattag gattgctcca gctaatgcag
     8041 taaaaggaat taaatactta tagtttggtg gtaataatcg tttgctaata ttcggtacga
     8101 taagacccac aaagacgatt gatccagcta cggctaccga aataccggct aacatactga
     8161 tgagcataat aatcatccat ttgattaatt ttatgttttg accgaggccg gttgcaatgt
     8221 cgtcacttgt catcaagatg ttgatgtgtg cagccatgct aaatgcaatt aaaataagta
     8281 tcaatacaag cggaataatc catgggatat cccaaatatt acgtaatgaa acggagccac
     8341 ttaaccaaaa taataggcct tgtaagtctg tttcgttcat aataagtatg ccttgagtaa
     8401 aggctgtaaa tagcatcgca atcgcagcac ctgccaaaat gacacggtga ggtgagaata
     8461 gtgtttgtct aaacatacct agtgcaacaa ctaatacagt aacaacaata gcccccaaaa
     8521 atgcaataac tacaatcatt ttaaaagatt gaatttggat aaatgtaata ctaaaaatga
     8581 caaaaaatac tgcgcctgca ttgacaccga aaagccctgg tgaggctatt gggtttcgtg
     8641 taagtgcttg catcaacaaa cctgagacag caagggcagc accagtcaat aacgcaatga
     8701 ttgttctcga cgcccgtgca ccagtgacaa catcatgtaa atcgttttca ctatcaaagt
     8761 tgaataacgc ctgtatcacc gtacctggtg acacaagcgt atttccaatc attaaactta
     8821 agatagctac tattgcaaga cataaaccag caataacgat ttggtatttt ggtttaagta
     8881 gcatcgtaaa actccttaat tattttgatt gtttttcaat atttaacttt tcatataaat
     8941 cgtcaataag ttttaatgaa gatttatatc cgccagctaa gttccaagtg atttcatcta
     9001 aatcatcaga tacttggttg tttttaactg cgtctaaatt tttccactct ttacttgaag
     9061 tccattcgct ttcagtcttt ttaactaatg cagcatcttt cgcatttgga tctgatttta
     9121 ctacaaaaat atgatcagcg ttcattaatg gaatgctttc tttagatgta agttggataa
     9181 tatctttacc attatcaact tgtttttgta agtctttatt acgtttgaat cctaaatcat
     9241 ttaagatttc accagcatat ccaccagcat aaattcttgt atgatcagca cggaagttaa
     9301 caactgaagc tttcaatggc catgcatctt tatactttgc ttttgcatct ttttggaatg
     9361 cagctacttt atcatcgtac tttttaagta aatcttcagc ttctttttct ttccctaaag
     9421 ctttccccat taacttagtt gtatctttga atttgaaaac tgtatcagta gaaactgttg
     9481 gtgcgatttt agataattga tcgtaaactt tttcatttct aacttttgac gcgacaatta
     9541 agtccggttt taatttagag atttcctcta agttaggtgc aggttcttga cctacaatct
     9601 tagtatcttt taaatcattt tttatgtatt cgaatttcgg tttttgtgtc catgattcta
     9661 cagcacctac aggtttaaca cctaaagata cagcgacgtc agtggcacct tgatatagcg
     9721 taacaacacg ctttggtttc cctttaattt cagttgtacc cattgcatgt ttaattgaag
     9781 ttgtttcctt atctttgtta tcagatgatt gtttatttga attcccacta catcctgcta
     9841 aaacaagtag gaaagcaagc gtaacaacaa gcattttaat tactttattc attgactaat
     9901 tagcctcctt cgtgatgtat gacaatgaga atcattatca cgatttagta tgaattaaat
     9961 tttttcctaa gtcaataaaa tatttatgat ttacatgcaa cttataatta tttgacatat
    10021 aaatgcataa aaaatataat cctaattact tgatagtgag aatcattatc aattaggtaa
    10081 cacacaatat tatagaattt taaatttgag gaggaagcgc ttttgattga aaaaagtcaa
    10141 gcatgtcacg attcattgtt agattctgta gggcaaacac ctatggttca acttcatcaa
    10201 ctatttccga aacatgaagt gtttgcaaag ttagagtata tgaatcctgg aggcagcatg
    10261 aaagatcgac ctgccaagta catcattgaa catggtatta aacatggttt aatcactgag
    10321 aatacacatt taattgaaag tacttctggt aatttaggca ttgcgttggc aatgatagct
    10381 aaaatcaagg gattaaaact cacgtgtgtt gttgatccta aaatatcacc aacaaatttg
    10441 aaaattatta aaagttatgg tgccaatgta gaaatggttg aagaacctga tgcacatggg
    10501 ggttatttaa tgactcgtat tgcaaaggtg caagaactgt tagccactat tgacgatgca
    10561 tattggatta atcaatatgc gaatgagtta aattggcaat cccattatca tggtgcaggc
    10621 acagagattg ttgaaacaat taagcaacct atagattatt ttgtcgcgcc agtcagcacg
    10681 acaggtagca ttatgggtat gagtagaaaa ataaaagaag tgcatccaaa cgcacaaatt
    10741 gttgctgttg atgcgaaagg gtcagtcatt tttggtgaca aacctattaa tagagaatta
    10801 cctggtatcg gtgctagtcg tgtacccgaa atattgaata gatcagaaat taatcaagtg
    10861 atccatgtag atgattatca atctgctttg ggctgtcgaa aactgattga ttatgaaggc
    10921 atatttgccg gaggttcaac aggttcgatt attgcagcga ttgagcagtt gataacgtca
    10981 attgaagaag gtgcaacaat tgtcacgatt ttaccagatc gaggcgatcg ttacttagat
    11041 ttagtttatt cagatacatg gttagaaaaa atgaaatcaa gacaaggagt taaatcagaa
    11101 tgaatagaga gatgttgtat ttaaatagat cagatattga acaagcggga ggtaatcatt
    11161 cacaagttta tgtggacgca ttaacagaag cattaacagc ccatgcgcac aatgattttg
    11221 tacaaccgct taagccgtat ttaagacagg atcctgaaaa tggacacatc gcagatcgaa
    11281 ttattgcaat gccaagtcat atcggtggtg aacacgcaat ttcaggtatt aagtggatag
    11341 gtagtaagca cgacaatcca tcgaaacgta atatggagcg tgcaagtggc gtcattattt
    11401 tgaatgatcc agaaacgaat tatccaattg cagttatgga agcaagttta attagtagta
    11461 tgcgtactgc agcagtttca gtgattgcag caaagcattt ggctaaaaaa ggatttaaag
    11521 acttaacaat cattggatgc gggctaatcg gagacaagca attacaaagt atgttagagc
    11581 aattcgatca tattgaacgc gtgtttgttt acgatcaatt ctctgaagca tgtgcacgct
    11641 ttgttgatag atggcaacaa cagcgtccgg aaattaattt tattgcgaca gaaaatgcta
    11701 aagaagcagt atcaaatggt gaagtagtca ttacatgtac cgtaacggat caaccataca
    11761 ttgaatatga ttggttacaa aagggtgcat ttattagcaa catttctatc atggatgtgc
    11821 ataaagaagt ctttattaaa gctgacaaag tcgtagtaga tgactggtca caatgtaatc
    11881 gagaaaagaa aactattaac caattggtgt tagaaggtaa attcagcaaa gaagctcttc
    11941 atgctgaact aggacaactt gtgacaggtg acataccagg acgtgaagac gatgatgaga
    12001 tcatattact taatccgatg ggtatggcta tcgaagatat ttcaagtgct tattttattt
    12061 atcaacaggc acaacaacaa aatattggga caacattgaa cctatattaa gaatgcgagg
    12121 tgtctgaaca ttgcagaatc atacagcagt caatacagca caagcgataa tattaagaga
    12181 tttagttgat gcattattat ttgaagatat agccggaatt gtatcgaata gtgagattac
    12241 taaagaaaac ggacaaacgc ttttgatata cgaacgtgaa acacaacaaa taaagatacc
    12301 tgtttatttt agtgctttaa atatgtttcg ttacgaaagt tcacaaccaa ttacgataga
    12361 gggaagggtg tctaagcaac ctttaacggc agctgaattt tggcaaacaa ttgctaatat
    12421 gaattgtgat ctaagtcatg aatgggaagt ggctcgcgtt gaagaaggac tgactactgc
    12481 tgccacacag cttgctaaac aattatcaga attagattta gcgtcacatc cttttgtgat
    12541 gtcagagcag tttgcaagtt taaaagatcg tccatttcat ccattagcta aagaaaaaag
    12601 aggattaaga gaagcggatt atcaagtgta tcaagctgaa ttaaatcaat catttccttt
    12661 aatggttgca gcagttaaaa agacacatat gattcatggc gatactgcaa atatcgatga
    12721 attagaaaat ttgacagtac ctataaaaga acaagcgaca gacatgttaa atgatcaagg
    12781 gttatcaata gatgactatg tactatttcc ggtacatcct tggcaatatc agcatattct
    12841 gccgaacgtc tttgcgaaag agattagtga aaagttggtt gtactattac cgttaaaatt
    12901 tggagattat ctgtcgtctt caagtatgcg ttcattaatt gatattggcg caccgtataa
    12961 ccatgtcaaa gtaccatttg caatgcagtc attaggcgca ttaaggctaa cgcctacgcg
    13021 ttacatgaaa aacggagaac aagcagaaca attattacgt cagcttatag aaaaagatga
    13081 agcactagct aagtatgtca tggtttgtga tgaaacagct tggtggtcat atatgggtca
    13141 agataatgat attttcaaag atcaattagg tcatctaact gttcagctaa gaaagtatcc
    13201 cgaagtgcta gccaaaaatg atacgcaaca gctagtgtca atggcagcac tcgcggcaaa
    13261 tgatcgcact ttatatcaaa tgatttgtgg aaaagataat atttctaaaa atgatgtcat
    13321 gacgttattt gaagatatcg cgcaagtctt tttaaaggta acactatcat ttatgcaata
    13381 cggcgcatta ccagagttgc atggtcaaaa tatattgttg tcatttgaag atggacgtgt
    13441 acaaaaatgc gtgttacgtg atcatgatac tgtcagaatt tataaaccat ggctaacagc
    13501 acatcagctt tcattgccga agtatgtcgt cagagaagat acacctaata cgctaattaa
    13561 tgaggatttg gaaacattct ttgcttattt tcaaacatta gctgtatcgg taaatctata
    13621 tgccattatt gatgcaattc aagatttatt tggtgtaagt gagcatgaac ttatgtcgtt
    13681 gttaaaacaa attttaaaaa atgaagtggc aactatttcc tgggttacaa ctgatcagct
    13741 agctgtcaga cacattttat ttgataaaca gacgtggcca ttcaaacaaa ttttattacc
    13801 attgctatat caacgtgata gtggtggagg tagtatgcct tcaggtttaa ctaccgtacc
    13861 aaatccaatg gtgacatatg attaatcagt ctatatggcg cagtaacttt cgcattttat
    13921 ggctcagtca gtttatagcg attgctggac tgacagtact tgtgccatta ttgccaattt
    13981 atatggcatc actacaaaat ctatcagtcg tagaaataca gttgtggagt ggtatagcga
    14041 ttgctgctcc agctgtaacg acgatgatag cttcgccgat atgggggaag ctaggtgata
    14101 agatcagccg aaaatggatg gtgttaagag cgttacttgg tttggcggta tgcttatttt
    14161 taatggcatt gtgtacgaca ccattacagt ttgtacttgt gaggttattg cagggactat
    14221 ttggtggtgt tgttgatgca tcaagtgcgt ttgcgagtgc agaggcgcca gctgaagatc
    14281 gtggaaaggt attaggaaga ctgcaaagtt cagtcagcgc agggtctctt gtggggccat
    14341 taattggcgg tgttacagct tcgatattag gttttagtgc gttactgatg agtattgccg
    14401 ttattacttt tattgtctgt attttcggtg cattaaaatt gattgaaacg acacatatgc
    14461 caaaatcaca aacaccaaat attaataaag gtattcgccg ttcatttcaa tgtctattat
    14521 gcacacaaca aacatgtcga tttattatcg ttggcgtttt agcaaacttt gctatgtatg
    14581 gcatgctaac tgcattatca ccacttgctt catcagtgaa tcatacagcg atagatgacc
    14641 gtagtgtgat tggattttta cagtccgcat tttggacggc ttcgatatta agcgcgcctt
    14701 tatggggacg ctttaatgat aaatcatatg ttaaatcagt atatatattt gccacgattg
    14761 catgtggttg tagtgcgata ctgcaaggtt tagcgacgaa tatagagttt ttaatggctg
    14821 caagaatact tcaaggatta acatatagtg cattgattca aagtgtcatg tttgttgtcg
    14881 tgaatgcgtg tcatcaacaa cttaaaggca catttgttgg aacgacgaac agtatgttag
    14941 ttgttggtca aattattggc agtcttagtg gcgctgccat tacaagttat actacaccag
    15001 ctactacgtt tatcgttatg ggcgtagtat ttgcagtaag tagtttattt ttaatttgtt
    15061 caaccatcac taatcaaatc aacgatcaca cattaatgaa attatgggag ttgaaacaaa
    15121 aaagtgcaaa ataaagaatt aatacaacat gcagcgtatg cggctatcga acgcatttta
    15181 aatgaatatt ttagagaaga aaatttatat caagtaccac ctcaaaatca tcaatggtct
    15241 atacaattat cagagctcga aactttaacg ggtgaatttc gctattggtc tgcgatgggg
    15301 catcatatgt atcatccaga ggtatggctt atcgatggaa aaagtaaaaa aataacaact
    15361 tataaagaag caattgcgcg tattttgcaa catatggctc aaagtgcaga taatcaaacg
    15421 gcagtgcaac aacatatggc gcaaattatg tctgacatcg ataatagcat tcatcgcacg
    15481 gcacgttatt tgcaaagtaa cacaatagac tacgtagagg atcgttatat cgtttcagaa
    15541 caatctttat acttaggtca tccatttcat ccgactccta agagtgcaag tgggttttca
    15601 gaagcagatt tagagaaata tgcacccgaa tgtcatacat cattccaatt gcattattta
    15661 gctgtgcatc aagatgttct gctcacgcgc tatgtagaag gtaaagaaga tcaggttgag
    15721 aaagtgttgt atcaattagc agacatagat atatcagaga tacccaaaga ttttatttta
    15781 ttaccaacac atccttatca aatcaatgtg ttgcgacagc atccacagta tatgcaatat
    15841 agtgaacaag gtttaataaa agaccttggc gtttccggtg attcagtgta cccgacgtct
    15901 tcggttagaa ctgtattttc aaaagcatta aacatttatt taaaattacc gatacacgtt
    15961 aaaatcacta attttatacg tacgaatgac cttgaacaga ttgaacggac aattgatgcc
    16021 gcgcaagtta tcgcatcagt caaagatgag gttgaaacac cccattttaa attgatgttt
    16081 gaagaaggat atcgtgcatt gttaccgaat ccattagggc aaacagttga acctgaaatg
    16141 gatttattaa caaatagtgc catgattgtt cgtgaaggga taccgaatta ccatgctgat
    16201 aaagatattc atgtattggc gtcattattt gaaacgatgc ctgattcacc gatgtctaag
    16261 ttatcacaag tgattgagca aagtggttta gcaccagaag catggcttga atgttatttg
    16321 aatcgtacat tattgccgat attaaagctg tttagtaaca caggcattag tctagaagca
    16381 catgtacaaa atacattaat tgaattaaaa gatggcatac ccgacgtatg ctttgtcaga
    16441 gatcttgaag gcatttgtct atctagaacg attgctactg aaaaacagct tgtgccaaat
    16501 gttgtggcag catcaagccc tgttgtatat gcacatgatg aagcatggca tcgtcttaaa
    16561 tattacgttg tagtaaatca cttaggacat ttagtatcaa ctattggtaa agcgactaga
    16621 aatgaagttg tgttatggca acttgtagcg catcgtctta tgacttggaa aaaagaatac
    16681 gcgaataacg cagtatttgt tgactgtgta gaagatttat atcaaacgcc gaccattgcg
    16741 gctaaagcga atttgatgag taaattgaat gattgtggtg caaaccctat ttatacacat
    16801 ataccaaatc caatttgtca taacaaggag gtatcgtatt gtgaatcaaa caattcttaa
    16861 tcgtgtaaag actagagtga tgcaccaact ggtatcatca cttatttatg agaatattgt
    16921 tgtgtataaa gcgtcatatc aagacggtgt cggtcatttt acaatagaag gacatgattc
    16981 agagtatcgt tttactgctg aaaagacaca tagctttgat cgtatacgta tcacatcacc
    17041 aattgagcgt gtcgtaggag atgaggcaga tacaacaaca gactatacac aattattgag
    17101 agaggttgta tttacatttc ctaaaaatga tgaaaagcta gaacaattta ttgtcgagtt
    17161 attacagaca gaattaaaag atacgcaaag tatgcagtat cgagaatcaa acccaccagc
    17221 aacacctgag acatttaacg actatgaatt ttatgcgatg gaagggcatc agtatcatcc
    17281 aagttacaaa tcacgtttag gatttacgtt gagtgataat ttgaaatttg gtcctgattt
    17341 tgtaccaaac gttaaactgc agtggttagc tatcgacaaa gataaagtag aaacgacggt
    17401 atcaagaaat gttgtagtta acgaaatgtt acgtcaacaa gttggcgata agacttatga
    17461 acattttgta cagcagattg aagcatctgg caaacatgta aatgatgttg agatgatacc
    17521 tgtacaccca tggcagtttg aacatgtcat ccaagttgat ttggctgaag aaaggcttaa
    17581 tggcacagta ctatggttag gggaaagtga tgagctatat caccctcaac aatcgattcg
    17641 tacgatgtcg ccaatagaca cgacaaaata ttatttaaag gtaccaataa gtataacgaa
    17701 cacttcaacg aaacgagtgt tggcgcctca tacaattgaa aatgcagcgc aaattacgga
    17761 ttggttaaag cagatacagc aacaagatat gtatttaaaa gatgaattaa agacagtttt
    17821 tctaggggaa gtcttaggac agtcttattt aaatacacaa ctttcgcctt ataaacaaac
    17881 tcaagtttat ggtgcgttag gtgttatatg gcgtgaaaat atatatcata tgttaatcga
    17941 tgaagaggat gcgataccat ttaatgcact ttatgcaagt gataaggatg gtgtaccatt
    18001 cattgaaaat tggattaaac aatatggttc tgaagcttgg acaaagcaat ttttagctgt
    18061 agcgattcgt ccaatgattc atatgcttta ttatcacggt attgcctttg aatcgcatgc
    18121 acaaaatatg atgctcattc atgaaaatgg ttggcctaca cgtattgcct taaaagattt
    18181 ccatgatggt gttcgtttta agcgtgagca tttaagtgaa gctgcttcac acctgacatt
    18241 aaagccaatg ccagaagcac ataaaaaagt gaatagtaat tcatttattg aaacagatga
    18301 cgaacgttta gtacgcgact ttttacatga tgcatttttc tttattaata tcgccgaaat
    18361 catcttattt attgaaaagc aatatggtat cgatgaggag ctgcaatggc aatgggttaa
    18421 aggcatcatc gaggcgtatc aagaagcatt tccagagttg aataactatc aacatttcga
    18481 tttgtttgaa cctacgattc aagttgaaaa gttaacgaca cgtcgattat taagtgactc
    18541 cgagttaaga attcatcatg ttacaaatcc attaggtgta ggaggtatca atgatgcaac
    18601 aactatctct gaaacataga ttaaacaatg gtgattcagt ttatggcatt tttaattcta
    18661 taccggaccc attgatgatc gaggttatcg cagcaagcgg gtatgacttt gttgtgattg
    18721 atacagaaca cgtggcgatt aatgatgaga cactagcgca tttaattcgt gcagctgaag
    18781 cagcgcatat tataccaatt gtacgtgtca ctgcagtgat agatagagat atcattaaag
    18841 tgttggatat gggtgcgaga ggtattattg tgccacacgt taaagatcgt gagacagttg
    18901 agcatattgt gaaattaagt cgttattacc cgcaaggatt aagaagtttg aatggtggtc
    18961 gcatggcaag atttggacgt acaccattac ttgatgcaat ggagatggct aatgagcata
    19021 ttatggtgat tgccatgata gaagatgttg aaggggttat ggccattgac gatatagcac
    19081 aagtcgaagg tttagacatg atagtcgaag gtgccgcaga tttatcgcag tcacttggca
    19141 taccatggca aacgcgtgat gatcaagtaa catcacatgt tcaacatatt tttgaagttg
    19201 tgaatgcaca tggtaaacat ttttgtgcat taccacgtga agatgaagat attgcaaaat
    19261 ggcaggcaca aggtgtacaa acatttattt taggtgatga tcgcggaaaa atatatcgcc
    19321 atttaagtgc atctctagcg acgtctaaac agaaagggga tgaaggctaa tgcgtatagt
    19381 tcaacctgtt attgaacaat taaaagcaca atctcatcca gtttgtcatt atatctatga
    19441 tttagtcgga ctggaacatc atttgcaaca tattacatcg tcattgccga gtaattgtca
    19501 aatgtactat gcaatgaaag caaatagtga acgaaaaatc ctagatacaa ttagtcagta
    19561 tgttgaagga ttcgaagttg catctcaagg tgaaatagca aaaggtcttg cttttaaacc
    19621 agcaaatcat attatttttg gtggccctgg taagacagac gaggaactaa gatatgcagt
    19681 aagtgaaggt gttcagcgta ttcatgttga aagtatgcat gaattacaac ggctaaatgc
    19741 catcttagaa gatgaagata agacacaaca cattttattg cgtgttaatt tagcaggacc
    19801 atttcccaat gcaacgttgc atatggcagg acgcccaaca caatttggta tttctgaaga
    19861 cgaagttgat gatgtcattg aagctgcgct cgcaatgcca aagattcatc tagatggatt
    19921 tcattttcat tctatttcta acaatttaga ctcgaattta catgtcgatg tagtgaaact
    19981 ttattttaaa aaagcaaagg catggtctga aaaacatcga tttccactca aacatatcaa
    20041 tcttggtggt ggcataggcg ttaactatgc agatttaact aaccaatttg aatgggataa
    20101 ttttgtagaa cgttttaaaa cacttatcgt tgagcaagaa atggaagatg tgacattgaa
    20161 ctttgaatgt gggcgcttta ttgtggcaca tattggttac tatgtgacag aagtgctaga
    20221 cattaagaaa gtacatggtg cttggtatgc catattaaga ggaggtacgc aacaatttag
    20281 actgccggta tcttggcaac ataaccatcc ttttgacatt tatcgctata aggacaatcc
    20341 atattcattt gaaaaagttt caatttcgag acaggacaca acgttagtcg gtcaattatg
    20401 tacaccgaaa gatgtctttg ctagagaagt acagatagac gcaatcagta caggcgacgt
    20461 tattgttttc aaatatgcag gtgcatacgg atggtctatt tcacatcacg atttcttaag
    20521 ccatccacat cctgaattta tttatttaac gcaaacaaag gaggatgaat aactattgaa
    20581 tcatattcat gaacatttaa aattggtacc agtagataag attgatcttc acgaaacatt
    20641 cgaaccttta agattggaaa aaacgaaaag tagtattgaa gcagatgatt ttatacgtca
    20701 tcctatttta gtgacagcga tgcaacatgg tagatatatg gttatagatg gtgtgcatcg
    20761 gtatacaagt ttgaaagcgt taggatgtaa gaaagttcca gtgcaagaaa tccatgaaac
    20821 acaatattca attagtacat ggcaacataa agttccattt ggtgtgtggt gggaaacgtt
    20881 acaacaagaa catcgcttgc catggactac tgagacaaga caagaagcgc catttattac
    20941 aatgtgtcat ggtgatacag aacaatattt gtatacaaaa gatttaggcg aagcacattt
    21001 tcaagtatgg gaaaaggttg tcgcaagtta tagtggttgt tgttctgtag agagaattgc
    21061 acaaggtaca tatccttgtc tttctcaaca agatgtactc atgaagtatc agccattgag
    21121 ttataaggaa attgaagcgg ttgttcataa aggggaaact gtgccagcag gtgtgacacg
    21181 ctttaatatt tcaggacgat gtcttaatct tcaagtacca ctggcattac ttaaacaaga
    21241 tgatgatgtt gaacaactgc gcaattggaa gcagttttta gcagataagt ttgccaatat
    21301 gagatgctat actgaaaaag tatacttggt ggagcaatag ttttactgtg atgttgaggg
    21361 aaatatgatg atttagcgta ttgatagcga aaatataata aaacaatata gtgtggagaa
    21421 cttttgatat tttataaata ttgaagttct ccatttttgt attttgcata taaaaattaa
    21481 ataaaataag gtatattaag gtaaagtata aattttaaat aaatggggaa tgagtatgag
    21541 ctcaattata ggaaaaatag caatttggat aggcatcgta gctcaaatat attttagtgt
    21601 cgtttttgtt aggatgatat ctattaatat tgctggagga tctgattacg aaacaatttt
    21661 tttattagga ttaatattgg ctcttttcac tgttttacca accatcttta ctgcgattta
    21721 tatggaaagt tactctgtaa tcggaggtgc actttttatt gtttatgcta ttattgcact
    21781 gtgtttatat aatttccttt cgtcaatttt atggctgatt ggtggtattt tgctgatttg
    21841 gaataaatac tcaaaagatg aatcgacaga cgaaaatgaa aaagttgata ttgaaagtac
    21901 agagaatcaa tttgaatcta aagataaaat cactaaagaa taaagagaat atttaaggta
    21961 aagtataaat tttaaataaa tggggaatag acatggaaaa aaatgtagaa aaatcattca
    22021 taaagatagg tttatatttt caaatagctt atatagtact catggctata actttatgtg
    22081 ggtttgtaat ttgctatgga ctaattttcg gccttttcta tttattatca ggtagcagag
    22141 ctgattattt aatagtaaca atagttatat cggcaataat ttctatattt gtaattatac
    22201 tttcaatcgt acctgtcatc gtattggcat ctgacttatt taaagaaagg atttcaaaag
    22261 gtgtcatatt aattgtattg gctattatcg ctttagtatt atgcaacttt gtatctgcaa
    22321 tactctggtt tgtttcagcc atatctattt taggtagaaa aaaattagta gctgcagcag
    22381 atactaccac tattcaaaaa agtaaaggga acgcaaatca agcatcacat aaagacacgt
    22441 gtaaaaagga acttgatagt caagacatga tggaacatcc tgaggttaaa aatcccacga
    22501 ctaaaaacct tgaaggattt aacgaagaaa tacataaaga tgaagctaca actaaagttg
    22561 tcagtgataa cacggaaccg cctattgaat caaaagacca tgtctcgaaa aaagattgat
    22621 gacaaactaa tcgagagact taaaaaaata atattcaaca taagaacttt taaaacgaca
    22681 tttaaacgca ttgccaatca ctaatggtag tgcgtttaac tataccttaa atatctgaat
    22741 attttgttaa atggagctac ctttgttgta ctattcaaat gaagaggagt aaaatgtaat
    22801 taaaggaaag aaatttgagg agtgatcttt atgacaaaca acaaagtagc attagtaact
    22861 ggcggagcac aagggattgg ttttaaaatt gcagaacgtt tagtggaaga tggtttcaaa
    22921 gtagcagttg ttgatttcaa tgaagaaggg gcaaaagcag ctgcacttaa attatcaagt
    22981 gatggtacaa aagctattgc tatcaaagca gatgtatcaa accgtgatga tgtatttaac
    23041 gcagtaagac aaactgccgc gcaatttggc gatttccatg tcatggttaa caatgccggc
    23101 cttggaccaa caacaccaat cgatacaatt actgaagaac agtttaaaac agtatatggc
    23161 gtgaacgttg caggtgtgct atggggtatt caagccgcac atgaacaatt taaaaaattc
    23221 aatcatggcg gtaaaattat caatgcaaca tctcaagcag gcgttgaggg taacccaggc
    23281 ttgtctttat attgcagtac aaaattcgca gtgcgaggtt taacacaagt agccgcacaa
    23341 gatttagcgt ctgaaggtat tactgtgaat gcattcgcac ctggtatcgt tcaaacacca
    23401 atgatggaaa gtatcgcagt ggcaacagcc gaagaagcag gtaaacctga agcatggggt
    23461 tgggaacaat ttacaagtca gattgctttg ggcagagttt ctcaaccaga agatgtttca
    23521 aatgtagtga gcttcttagc tggtaaagac tctgattaca ttactggaca aacaattatt
    23581 gtagatggtg gtatgagatt ccgttaataa tcatccacta atgataaata aatccttatt
    23641 gttaagttta atcacttagc agtaaggatt ttttagtgca cttagaaggg agtgtattgg
    23701 tagaaaatta ataagcgaag ttcttaagtg agttatgatg tcacagtcta atgcatcagt
    23761 tgaaagcatt attagtatta acacacccaa gatattataa aacatcacaa aaacaccact
    23821 atctaattta tctcaataaa aattcacaaa gttatctcat tttattttta taaataaaaa
    23881 atatcgataa aaagcttaca atactttatg tttttatgat atatttttaa tgtataaatg
    23941 aggtggaaga tttggaaaga gttttgataa ctggtggggc tggttttatt gggtcgcatt
    24001 tagtagatga tttacaacaa gattatgatg tttatgttct agataactat agaacaggta
    24061 aacgagaaaa tattaaaagt ttggctgacg atcatgtgtt tgaattagat attcgtgaat
    24121 atgatgcagt tgaacaaatc atgaagacat atcaatttga ttatgttatt catttagcag
    24181 cattagttag tgttgctgag tcggttgaga aacctatctt atctcaagaa ataaacgtcg
    24241 tagcaacatt aagattgtta gaaatcatta aaaaatataa taatcatata aaacgtttta
    24301 tctttgcttc gtcagcagct gtttatggtg atcttcctga tttgcctaaa agtgatcaat
    24361 cattaatctt accattatca ccatatgcaa tagataaata ttacggcgaa cggacgacat
    24421 taaattattg ttcgttatat aacataccaa cagcggttgt taaatttttt aatgtatttg
    24481 ggccaagaca ggatcctaag tcacaatatt caggtgtgat ttcaaagatg ttcgattcat
    24541 ttgagcataa caagccattt acattttttg gtgacggact gcaaactaga gattttgtat
    24601 atgtatatga tgttgttcaa tctgtacgct taattatgga acacaaagat gcaattggac
    24661 acggttataa cattggtaca ggcactttta ctaatttatt agaggtttat cgtattattg
    24721 gtgaattata tggaaaatca gtcgagcatg aatttaaaga agcacgaaaa ggagatatta
    24781 agcattctta tgcagatatt tctaacttaa aggcattagg atttgttcct aaatatacag
    24841 tagaaacagg tttaaaggat tactttaatt ttgaggtaga taatattgaa gaagttacag
    24901 ctaaagaagt ggaaatgtcg tgaaaatgac attgaagctg tccataataa taagggttat
    24961 gcctatcaaa gaaaattaga caaactagaa gaagtgagaa aaagctatta cccaattaaa
    25021 cgtgcgattg acttaatttt aagcattgtt ttattatttt taactttacc gattatggtt
    25081 atattcgcca ttgctatcgt catagattcg ccaggaaacc ctatttatag tcaggttaga
    25141 gttgggaaga tgggtaaatt aattaaaata tacaaattac gttcgatgtg caaaaacgca
    25201 gagaaaaacg gtgcgcaatg ggctgataaa gatgatgatc gtataacaaa tgtcgggaag
    25261 tttattcgta aaacacgcat tgatgaatta ccacaactaa ttaatgttgt taaaggggaa
    25321 atgagtttta ttggaccacg cccggaacgt ccggaatttg tagaattatt tagttcagaa
    25381 gtgataggtt tcgagcaaag atgtcttgtt acaccagggt taacaggact tgcgcaaatt
    25441 ca
//
LOCUS       DANRDI010000023        24671 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.023, whole genome shotgun sequence.
ACCESSION   DANRDI010000023 DANRDI010000000
VERSION     DANRDI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24671
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.023"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..735)
                     /locus_tag="PZ377_000730"
     CDS             complement(<1..735)
                     /locus_tag="PZ377_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDM6357618.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHY"
     gene            complement(753..3143)
                     /gene="secA2"
                     /locus_tag="PZ377_000731"
     CDS             complement(753..3143)
                     /gene="secA2"
                     /locus_tag="PZ377_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDM6357619.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(3133..4092)
                     /gene="asp3"
                     /locus_tag="PZ377_000732"
     CDS             complement(3133..4092)
                     /gene="asp3"
                     /locus_tag="PZ377_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDM6357620.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(4070..5638)
                     /gene="asp2"
                     /locus_tag="PZ377_000733"
     CDS             complement(4070..5638)
                     /gene="asp2"
                     /locus_tag="PZ377_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDM6357621.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(5628..7181)
                     /gene="asp1"
                     /locus_tag="PZ377_000734"
     CDS             complement(5628..7181)
                     /gene="asp1"
                     /locus_tag="PZ377_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HDM6357622.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKETDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(7192..8403)
                     /gene="secY2"
                     /locus_tag="PZ377_000735"
     CDS             complement(7192..8403)
                     /gene="secY2"
                     /locus_tag="PZ377_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDM6357623.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            complement(8533..15348)
                     /gene="sasA"
                     /locus_tag="PZ377_000736"
     CDS             complement(8533..15348)
                     /gene="sasA"
                     /locus_tag="PZ377_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDM6357624.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDLNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
     gene            complement(15893..16585)
                     /locus_tag="PZ377_000737"
     CDS             complement(15893..16585)
                     /locus_tag="PZ377_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDM6357625.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            16821..17279
                     /locus_tag="PZ377_000738"
                     /pseudo
     CDS             16821..17279
                     /locus_tag="PZ377_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17266..17568
                     /locus_tag="PZ377_000739"
     CDS             17266..17568
                     /locus_tag="PZ377_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357626.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            17722..18081
                     /locus_tag="PZ377_000740"
     CDS             17722..18081
                     /locus_tag="PZ377_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357627.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(18166..18777)
                     /locus_tag="PZ377_000741"
     CDS             complement(18166..18777)
                     /locus_tag="PZ377_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDM6357628.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(18856..19332)
                     /locus_tag="PZ377_000742"
     CDS             complement(18856..19332)
                     /locus_tag="PZ377_000742"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDM6357629.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(19335..19835)
                     /locus_tag="PZ377_000743"
     CDS             complement(19335..19835)
                     /locus_tag="PZ377_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357630.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(20070..20837)
                     /locus_tag="PZ377_000744"
     CDS             complement(20070..20837)
                     /locus_tag="PZ377_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDM6357631.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(20834..21526)
                     /locus_tag="PZ377_000745"
     CDS             complement(20834..21526)
                     /locus_tag="PZ377_000745"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDM6357632.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(21543..22205)
                     /locus_tag="PZ377_000746"
     CDS             complement(21543..22205)
                     /locus_tag="PZ377_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDM6357633.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            complement(23052..23612)
                     /gene="icaR"
                     /locus_tag="PZ377_000747"
     CDS             complement(23052..23612)
                     /gene="icaR"
                     /locus_tag="PZ377_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDM6357634.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            23776..>24671
                     /gene="pgaC"
                     /locus_tag="PZ377_000748"
     CDS             23776..>24671
                     /gene="pgaC"
                     /locus_tag="PZ377_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDM6357635.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIDENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQI"
BASE COUNT         7592 a         4555 c         3819 g         8705 t
ORIGIN      
        1 ataatgatca gcatgaatca caacaccaac tttactatca cctttatgct gcaaaacagc
       61 ctgaccaata tcagaagcgc ggtctaatat gacaatatcg tctcgggtta aattcaatcg
      121 ttgtaaaaag tatgcaataa attccgtttt gttatacaac accgcatctt caaacacata
      181 tatagagctg tctccatcaa tatattcgtt ataagcgatg gaaccatctt gattataaaa
      241 ttgtcgcata tataatttcg ctttattatc agctggtgca taatactcag aaaatatgcg
      301 cgtataacta taaaaatctt tacgtactaa catactatta attacaaatt ctgcacgatc
      361 cacaatatct ttttgttcat tttgcagata acatgttaca aatgatgatt tcccattaaa
      421 atataggcgg actatcttac catttctttc tctaaaacta atgtcatgac caagctcacg
      481 ttcaatgtca tctaacgtgt acgttgttgg tgctaaagaa atatcactaa aatactgata
      541 caaccaaata acttcttgat ctttaaaccc aatgttttgc gttaatgtct gtatgttctc
      601 tgactgtata aaatctaaaa acacaaattt agtgtcttga tttgtacgtc tcaataattt
      661 agcacggtaa gcttgtgcat attcaacacc gctactcgcc cagcctatac caaagtttat
      721 attatatatt gtcatgcgct accccttttc atttatggaa aatgtataac tggcataccc
      781 tctttatcaa atgtaatcat gctttgacaa atatttttca ccattctttt tttgatattt
      841 cgtgtcataa cttcaaatga atctaaggca actctatggt attcaaaaat agcgttacgt
      901 tgcccatttt gtcgttgatt aacgcttgct tttaattgtt gtaaatagtc gacttgttct
      961 aaccaacatg aatcaattgc tttcaaaaag actttttgaa cgaaaatatt ataataatat
     1021 gcactttgca tgtttttacg attcaaagct aattgctttt caaattgctc taataaaaat
     1081 gtcactactg cttgcttatc tttaaaatta acacaagcca catctttatt aaattggaaa
     1141 cttaaatttt gataaatata ctcgacaaca cgcgattttg ttagcacctt ttcctcattt
     1201 acaaacattt caaatacatc tttagctaac gctttaaaat cttgattctc agcatcatct
     1261 atttctaaaa ctcgattgcg ttcctcgtat acaagatctc gctgtatact aatgcttttt
     1321 tcaaattcat tagccatttc acgagcttta accccttgtt cttccgagat acgctgcgct
     1381 ttaactacaa tttgcttaac tttgcgatta aacaaattac tttgcgataa tcgttgtgca
     1441 tctaatgaat ataattgatt attttccgct aaattactat cgctccatcg cttaactaaa
     1501 taatcatcta gtgaaatata tatacaagat gatcccggat ccccttgtct accagaacga
     1561 ccacgtaatt gcctgtctac acggctattt tccatatgtt catgaataat aacagctaat
     1621 ccacctaatg cttcgacacc ttcaccaagt ttaatatctg tgcctcgacc tgccatacta
     1681 gtcgcaacag tcatggaacc aatttgccct gcttcagcta tcatctgcgc ttcttttgca
     1741 acattttgcg caatgagtaa attattagga atatccattt ggaataatac tttcgaaaag
     1801 tattcagccg cttcagcagt tctcgttatg agtaaaaccg gtcgccccgt ttcatgaagt
     1861 tcaactatat catgaatcat cgcgatgttt ttctcatcaa ctgaacgaaa cactttatct
     1921 ggttcatcga tacgttgaat cgctttatca gttggtactt gtacgactat ttttgaatac
     1981 aaatcaaaga actctgattc gcctaatttt cctgtagctg tcatacctga aaatgattca
     2041 aaaagtttaa ataaattctg gaaggtaatt gttgccataa cacttttatc tgttgaaacc
     2101 tccatacctt ctttcgcttc aatagcttgg tgaagtccag cttgcaactt agttcccggt
     2161 aacatacgac ctgtaatacg gtcaattaaa acaatatcac cattatatac aaagtaatcg
     2221 acattagatt caaacaaata ttgtgcgcgc agtgctaaat taatattacg cactaggacc
     2281 atcgcttgtt cgctatataa atcttcaaca ttaaagtatg attgtgccgc ttcaatacct
     2341 tgatttaaca gccatatttc ttttttggtc ttcttcattt taaaatgcac gtcttcaatc
     2401 aatgtatcta caaactcttt cacaatatga aatagatttg attgtaatct tggtgcaccc
     2461 gaaataacta atggtgtttg agcagcatct aaaatgattg aatccacttc atcaataata
     2521 ccgtaattta attgtggtaa aaatttccct tccgcactat cagccaaatt atcaattaaa
     2581 taatcaaaac cgagacgtcc attagttgta tatataatat catgttcata tatattacgt
     2641 ttttcccctt tttgatactc ataatccaca atatcaacaa aacctaatga agcagttaaa
     2701 cctaaccatt catataatgg ttgcatctct tcaaaatctc gcttcgctaa ataatcattc
     2761 gttgtaatta aatatgttcc ttttcccgaa agagcattta aatataaagg catcgttgcc
     2821 gttaatgttt taccttcgcc tgtttgcatc tccgcaatgt taccttcatg caatacaatc
     2881 gctccgatta actgaacttc tttaggatac atacctaata ctctccagct cgcttcacgt
     2941 gccactgcat aagcttcagg taacaatgta tctagtgtat caactcctga tgctaaacgt
     3001 tctttaaatt ctattgtctt ttgttttaac gcatcatcag aatatgattt aacttcatcg
     3061 ctccatgtat tgatgcgttt cactattttt ctaatcgact ttagtcttaa ttcgtttatc
     3121 gtaacatcta gtttatgttt catttacttc cccaccattc agtttcgata catctaagta
     3181 atctaaaaat cgtactggat tcattaaacg tgacatataa tttagatgtt tgtcttgctc
     3241 ttctttaaaa taaacctcga catttgtatc ttttagttca tgatttcctg ggacatgttc
     3301 tgtaagccat ccttttaaat catcatcttc atggcttgta cgatacactt tgcaacccaa
     3361 atgctgagcg acataagttg caaaaacatt tgactttgac ccataactaa tcaaattaat
     3421 agcctttagg gtatcttgac tttgcaaatc attctttagt tgcttaatat ttccctcgat
     3481 attgtcgtcc atccaacgtt caacgagcca aacatgacca aacagtttca aaaaatcatt
     3541 cgaaatagtt ggataggtgt cagatggttc tgcaataatg acattgatca tatcatttcc
     3601 atattggtca tcgcctatct tcgtcacccg catgctttta tactctaaat catattgatg
     3661 cgtcatctct gtgattgtta aacatctaaa tataagactc gtcgatgctg cattcatcat
     3721 ttttatttta taagcatagg cttcatcagg atattgaatc gtaatactat ttgactttac
     3781 aatctcagta cttagttttg tgccattttt attataaaaa atgatgataa aatacactga
     3841 accagcaggc gttgcatcaa aatcaaaatg caatttataa tgctgtcctc tacgcaaaat
     3901 tggtaaactt ggcgcacttt tatattttga aaattgcttt aacatcaacc actcatgaat
     3961 cggtaatcca gagggcatca aaggatttat aaaagtcact tcaccatttg aaaatgatac
     4021 tttagagcca tacataaatg tagtttgtga aatataattc caagtaactt taaatgtttt
     4081 gtttttcagc atgttgaact ctcccaaact tgtcttccaa aataatgttg taaaaattaa
     4141 caaaccaact tgcaatggta ggtgaatcat cattatgtcg cccaggaata ctgcgattca
     4201 tcactcttgc ttggtgtgct gtcaatacag gtaatagctc ttgaaatgca tgtggatcat
     4261 aatcatcatg ttgcatatat gctatggcaa aaacagtttg tgacaatgat ttcttttgaa
     4321 atgtttgcca aaatttttga tttaatgcct gtatcgacgc ttgagatgta tcaccttcat
     4381 tagacaccag gacgtctaat gctgtaccga actcttctgg tctaagtaat cgcatatgtt
     4441 cagcaatcgt tccaatatta acaagtggtt taccaacaat aattgcctga ggatttaact
     4501 gagcaccata atacaaggca ccaaatgaac ccatagataa accagataat attaattcat
     4561 gtgatttaaa attcagcttt tctaatgtct cgtcaataac attgataata ccttgttcat
     4621 attcagatga accgatataa aaactaccac cttcaacacg aggatcgccg ataagtaaaa
     4681 acggtgcatt catacgtttc atcatataat atccttcgaa accttccgct gttcgataac
     4741 cactaaaata tacgtttagt ggcggtttca tatcaccagg gtggaaataa taaataaatt
     4801 cctgtcgttg actatctacg aaacgactac caccaagtaa aaattgaccc atgtctaatc
     4861 tagaccatcg tttgtgtata ggtcctaaat gtaccgtccc gttcccacgc gccttaacag
     4921 ttacacttat ataagcatca aatggtttcg caggtatctc taaaggactg tctaacatat
     4981 catcagtcaa tacgatttgt tcaattaatg caccatcagc gccagtctga atcaatctaa
     5041 atgtatattg caactcgacc gcaccatcaa tatcaaattc tggccatatt tgaatgactt
     5101 tatctttatc gtaaacgaga ttattttgcc aagatgcgat aggtttaaat tctttcccaa
     5161 attctccact caatgtgagc tctgaattac cttggtaaac gacatctcct ttaaaattcg
     5221 gatgcacaag tgctaactta ggagaaacct tatctccata ctgtcctgag aagctaactg
     5281 cctctaattt attattacgt tcttcaatat tccggtaatg taatggttga acaacgtatt
     5341 tttggacatt ttcgtcttgt tcatattcaa ctgaccaaaa tgattcatca acatacgtat
     5401 tgtatggttc gcttatcatt tgtaataaat tcgttaatgt ctccgagtat ggtgcttgaa
     5461 tatagataaa atcaaagcgc ccttctgctt caacaatcgc ttcaatagcc tctacataac
     5521 cactatcaaa ttcaaacaat ccaatatcga agtaatccca actcacacct tttttgtgtt
     5581 gaaaaatagg ttctaaatcg tctcctccaa tttgcaaaac tctaaattta cgtggcatca
     5641 ttttcacctt ctattaactc atcgagctga ttaataatat tcttagaagc atatgcatct
     5701 attaatttta aagaataggc gtacgcataa ttccaatttt tcaaataaaa taaataataa
     5761 tttaacgcat catctaattc atcaactgta tttataatac ggccattgtc ataatcagag
     5821 acgtaatctg tttgttgacc attaatttgt ggaatcccag cgctaattgc actaatttgt
     5881 aaatacaagt cagtttcttt tgacatatct atcacaagtc gcaacgtccg caatgcttct
     5941 acaacatcat gttcagcatg tatcgtctta acagcaatga tgtcatcttg atcttcaggt
     6001 gtcattaatg ctgaaacatt aacatccgca ttctgtttag cttggtattc ctcatttacc
     6061 gacgtaatac attcacgaag ccacatcggt atgtcatttt gatggcgcga taataaaatt
     6121 aaacggtaat aatcttcctg tgcgatataa tccacaagtc gttgcatcat ttgttgcaaa
     6181 tcagcgtcac tcataccatc tatccataca cctataaatg tttccatcaa ttgactactt
     6241 atattaggtg attgtctcgt ttcaaatggt gtgattcgaa tcattgtatt ctcccgctga
     6301 tacttctctt gatgactaat taaatcacgt tctagttgca cactatcaac aattaaatgg
     6361 gcattcttta cgatagattg atattcctca tctgacacag tttcatttct atttttaaaa
     6421 aatgaataac ttaatgattt cgctggaata tgattggcta tttgtcgatt gtgcctagca
     6481 tctgaagcca caatcacatg atcatcttca tgtatttgtt gtgcaatcat tgcttgaaat
     6541 ttttcttcaa ttagttgagc catattgtta tattctgttt gttgatagtg atgttgatat
     6601 ctttttgaaa cagtgactct gccatttttc aaatcttcat gaagtacaca atctccatta
     6661 atcgttaaat attcttggta agaagcctct ccctgatcat caaaataacg tatcgctgat
     6721 aaataacctc tgtcatcaaa aatataacgc cgttgtaact gatctctttc aaattcttca
     6781 aaccaaattg aatacccttc ttgactaaaa taaatatttg tataggtctg ttcactcgtc
     6841 acacatttta ataaatacgg tgtgtacaca aactcaacat catccggcca ttttaagtga
     6901 tgataattaa tcgcttgtgg cgcatggtga ctgaatcctt gaatttcatc aaacacagac
     6961 gaatactttg tctcatataa gtcatatcga tgtaaaaatg ttcttaaatt tggtgcatga
     7021 ttgagaacaa tcagttgata atctaagtca ttttcaaggt gcattcccat taaactaatc
     7081 atatcgtcaa attccgtctt attttgtagt tgataatacg gcacagtcgt gtcttgccac
     7141 catcgttggt catcgtacca agctggaata aagtatttca taattacctc cttaccaata
     7201 ctggtttaaa aatggcttat atttatcaaa atataaatat gtacgaattg tttctgcaat
     7261 attaatactg atgtaaacta atacaatcag ttgtactgag aaataaattt cagtagataa
     7321 atgcggtaca aacaatgtga aataaagcgg tataccaata atgactgtaa ctaatgccaa
     7381 tccaaaccaa catacgcgtc gtgcttgata atttaaataa cgttctgtat ccttaccagg
     7441 tttaactcct gaaaaataat tgccactctt taagaaatct ttggattttt gtttagtatt
     7501 gattaaaaat ctcgataaaa aataacccaa taacatttga atcactaaat atactgaaat
     7561 acctactgga ctatcaaatg tcagcattgg catgtcatct gatatgcttt tattaaacat
     7621 agataaaata aaatgaatgc cactttttaa gaaaacaaaa gctgaaatac tcatcattaa
     7681 agtaatactg cctgcagggt taactttcca agataaataa gatttcatat ttgttgcgga
     7741 aacgttcatt aaatcgatat atggtattct cacttctact aattcaataa ataataagat
     7801 aaacaatgtg attatcacaa ggatgattaa caacgcaatc acaatatgac ttgcatctat
     7861 atattccatt ttttgatgca tcattgattt aataatacta accattacaa tcggcattgg
     7921 tcctgcgatg ccgtagcgac tatttttgtc agctaaccaa actaataaca tcgttccagt
     7981 aaccaaaatc aatattgtta agtaaatatt gtcttgatga acacgttctt tcgaaacata
     8041 ttcatgaatc acaaaataac tttgaataac acttaaaatt aatgttaaga tgcgctcttt
     8101 ataatgcttc tcaagacttg tttgtttcat gtatttatcc atattccgat aagaaatcag
     8161 catcaagata atcattgatg ttaaccacgg acctaatcct aaagtgaaaa tgtttaaagt
     8221 attaacgtct ccacccatat tagaaatagc tattttaaaa aatgactcat gttttacttg
     8281 catatcgtta taggaaacga tggaaatgtt tgtgcctaat atataaataa acaagataaa
     8341 acatgtgtat agcatacgtt tatatataat tttatattcg tattgttgta aaagttttaa
     8401 catgttgcac ctcttttata tcaaaaacat taaaaagact aagggttcat cactaattat
     8461 taaaatccta tatcgatttt tctagtgatt ggtgcctcag tctttttaat tttagccagc
     8521 tataaattca atttatgctt gagaatcatc ttgatcattt tcatctttct ttttctttct
     8581 cttcattaaa cctaaaccaa ctaataatgt cataacgcca cctagtaatc cattttgttt
     8641 tattgagtca cctgtatctg gcaatctttt ttcactttgt gctggtgtgc cattatgttt
     8701 agtcacttca gatgttgcac ttaatgtaga ctgagattca ctcgtgctcg ttgttgcttc
     8761 acttgataag cgagatgtgc tcgtgctgtg agtatgatgc atactcattg agtctgacgg
     8821 atgcattgag ttagattcgg atgtacttgt tgagccggac atacttgttg atgttgagtc
     8881 agaaatgctt tgtgaaccag acatagatgt actcagtgat tcggatgtgc ttgtcgaatc
     8941 ggatgtgctc aatgacgttg atgtgcttgt tgacactgat tctgagtcac taattgatgt
     9001 tgagtcggat ttgtcttgtg acattgaaac actcgatgaa ttagattcac tcattgatgt
     9061 tgagtcagat acgctcgttg aacctgaacc agacgtactt aatgattcag atatgcttgt
     9121 tgaagttgaa ccacttgttg agtccgatgt acttgtcgat gtcgagtctg aatctgatgt
     9181 actcaatgat tctgagtcac tgatagaagt tgaatcactt gtagattctg attctactgt
     9241 actttgtgaa ccactgatac ttattgaagt agaatcactg atactgtctg atgttgataa
     9301 tgatgtcgac accgatgtgc tttgtgatga cgatgtacta gcactcattg acattgatgt
     9361 tgatatcgat gtacttaagg aaccagatgc acttgtactt gttgactggc tttgtgacat
     9421 tgaatcactt aatgatgtag atgtgcttgt tgagctcgag tcacttacac ttgttgaacc
     9481 tgatattgag tcacttaaac ttgtcgatgt tgaaactgat acgcttccgc tcattgagtc
     9541 agatgttgaa agtgatgtac tcgttgaatt tgatccactg atgctagacg aatcacttgt
     9601 agacattgag tcgctttctg atgcactgat gctcatagag tcaaattgac tattacttgt
     9661 tgagcttgac tgcgaatcgc tcacacttgt tgacgttgat tctgatccac tcatactttg
     9721 cgagctactc aatgattttg aatcacttaa tgaatccgaa gtgctaagac ttgtggaacc
     9781 acttaaagat attgatccac ttaatgagtc ggagtcactt gtactagtag aatcactcat
     9841 tgatattgaa tcacttagcg aggtagactc gcttacgctt tctgaaccac ttaatgatgt
     9901 tgaggtactc aatgaaccag atgtacttgt tgaagtcgaa ccacttgttg attttgaatc
     9961 acttaatgaa tcagattcac tcacgctttc tgaacttctt agtgacgtcg atacacttaa
    10021 tgatgacgaa tcgcttgtgc ttactgaatc gctcatcgat tgtgagcaac tcaatgaact
    10081 tgactcgctt acactttctg attttcttaa tgacgttgag acgctcaatg agccagaatc
    10141 actgacactt gttgagccac tcatcgattt agagtcactt tcagaattag attcacttac
    10201 actttctgaa tcatttacag attctgacat actttgtgaa tcagatatgc ttgcgctcat
    10261 tacttcacta gccgatgttg atgtacttgt cgaatcactt aacgatatag acacactcat
    10321 cgaaccagat gtactcgcac ttgttgagtc tgatgttgaa tcactaacac tatcagataa
    10381 tgacgttgaa tcactcatac ttgttgatgt acttgtcgaa agcgacatac tttgtgaatc
    10441 actagtactt gtacgcatcg aagtactagt tgaagctgat gtactacgag agtcacttgt
    10501 tgatgttgat gtacttgctg atcctgatgc acttgtactt cttgatgtgc tttgtgaatc
    10561 gcttaatgat gttgatgttg aatcggattc acttgtactt tctgatgttg agccagactc
    10621 tgatgtactt accgatgtag ataaacttgc aatggtcgac atgcggtttg aagttgatgt
    10681 acttagcgaa tcacttaatg atgctgatgt gctttgtgaa tcggattcac tgctttgcat
    10741 tgacgcagat gttgaatcac ttaaagatgt tgacgacaaa tcggattcac ttgcgcttgc
    10801 tgagttactg agcgatgttg acgtacttgc tgatcctgag ttacttgtac ttgttgagtc
    10861 actgagtgat gttgacgcac tttctgatgt tgactcactt gcacttgttg agatactttg
    10921 tgaatcagat tcactctctg aaccacttaa tgacgctgat gtgcttaccg aacctgattg
    10981 actcgtactt ttagatatac ttgttgaatc gcttagtgac gttgatgtcg atgtggattc
    11041 actttggctt gttgactgac ttgtatatga tgatgtgctt tgtgaatcgg attcgctcgt
    11101 gcttgtactt gttgagtttg aggcactttg gcttgctgag tttaagtcta ctccgctttg
    11161 attcattgag gcacttagtg acaatgatgt actcgttgag tcagacaaac ttgtactcgt
    11221 tgacgtactt gtacttcctg atgttgattg agacatactt atgctcattg atgttgaatc
    11281 ggatttactt tcacttgatg atgttgagtc ggattcactt tcacttgtag aaccacttaa
    11341 tgatgtggat gtactaatgg aatcagattt acttgtactg attgaatcac ttgtcgacat
    11401 tgatgtactt aatgaatcag acttactatc acttgtggaa tcacttaatg atgttgacaa
    11461 acttgtagag tcagacaaac ttgtactcgt tgacatactc agtgatgttg aaacactctc
    11521 gctcttaaac gttgacgttg attcactgat acttgtcgat gttgaaatgg acgtactacc
    11581 acttgttgaa ttacttaatg atgttgatgt gctaccagat tctgatgtac tgtctgataa
    11641 tgacgtactc tcacttgtcg aactactcac tgactctgat gttgattcag acgtactttc
    11701 acttaatgat tcacttaaaa aggcagatgc actttgtgat tctgaatcgc tagtactatt
    11761 tgattcactt aatgatataa acgtgctctc tgaagcagat attgcttcac ttatagagtc
    11821 gctcgttgac gttgattcac ttattgaatc agactctgat gtacttaagc ttgtggaatc
    11881 actcatagat gttgatgtac gttctgaatt acttgtactt aacgatgtcg aagtacttac
    11941 cgaaccagat gtgctcgtaa aagcactttg tgatattgat tcacttgatg cagttgatgc
    12001 tgatttgcta tcactcgttg aatcactaaa cgacgttgat atgctcattg aatcggattg
    12061 acttgcactc attgaaccag acgtactttg tgattccgaa gtacgtactg aagcacttgt
    12121 cgacgttgat gtacttgttg aacctgattg actagtactt tgtgataatg acttactatc
    12181 agaatcagat gtactttgtg aatcacttaa tgattctgat gtactacctg actgagacgt
    12241 gctcattgaa ctacttacgg acattgattt actgtctgat gcagataatg acccacttgt
    12301 actgatagag tcacttacta tctctgaagt actcatcgag tctgatgtac ttgttgagac
    12361 actttgtgat gctgctatgc ttagtgatcc agaaacagaa ccacttgtgc tcgtcgaatc
    12421 gctcaatgat tctgatgtac tcatcgattt tgaatcactt gtacttaatg atattgatgt
    12481 actttgtgaa tctgatttgc ttgttgacgc actttgagag ttggctatgc tatttgaaat
    12541 actgatagag tccgaggtgc tagctgactc gctcaatgat gttgatgtac taattgcatt
    12601 cgatgtactg tcacttaatg atgctgatgt actagacgac cctgatatac tcgttgataa
    12661 gctttgtgac ttagacaagc ttcctgatgt actcatactt aatgagtcac tgagtgatgt
    12721 tgatgtacgc aatgaatcag atgtacttgt tgatagactt tcggattttt cagtactgct
    12781 agagtttgaa atagaatcgc ttaatgatgt tgatttacta gctgaatccg acatgcttga
    12841 tgatacactt tgtgaattca ctaaacttgt gcttgttgag cttgatacac tattactttc
    12901 agatgtgctt aatgacttag atgcactcac agaatcagat aggcttacac ttgtcgattt
    12961 cgaggtacta gctgatgtag ataccacaat cgatcctgat gtactcgttg atgcactttg
    13021 tgagtcagct ttacttgttg acacactttg agattgttgt gtacttcctg atgttgatac
    13081 ggaatcactc atgctatttc ttgttacttc atatttaaaa gttgtcgtcg ttttgttacc
    13141 gctcgcatct gtagaaacga ttgatatagt acttgtacca atgtttgttg gtgtaccact
    13201 aatagtatta tttgtactat caaatgttag tccggatggc aatccagtca ctgtattcgt
    13261 caccgcattt ccactgttat cttgcgtagc aattttaatc ggggatattg gtgaatacac
    13321 ttctgatgat tgatctccta ttggtgtcac tgttggtgct gtcgtatcca caacatttat
    13381 tgtaaaagtt gtcgtcgatt tgttatttgc ttggtcagta gacacaactg tcactgttga
    13441 ttgaccaatt tttgttggtg tcccaatgat tgaattcgtt gcactatcgt aacttaatcc
    13501 gcttggtaat cctgtaactg tatttgtcac agtcccagta ccattatccg ttgtagtcaa
    13561 tacaatagga ttcattgttt tacccacttc tatggtttga ttgcctacag ttacagttgg
    13621 tgcttttaca tcagtaaaat aatatgtcac tgattgtcca gcattcgtca tttttacagt
    13681 tttatttgta tcattataag ttgacgcata tgaactatcg acggacgtgt agttatatcc
    13741 tttagcagtc aatgcagatt gctgattatc gattgtcacg acttgatcaa catttcctga
    13801 atatgttttt ggtggaataa tatctttacc tgttgttaca tcaacgtatc tcacttgtgt
    13861 aacagcagac tctgtatatt cgaatgttcc aaattgtact tgttgtaaat ttgtcgcgcc
    13921 acctgttgag gctgtcattg ataatgaaaa gttggtcgta ccacttttcg caatccaatc
    13981 tgaaatatta cgtgtccatg tttgacctgc atatttgaca gtcataacct ttgtatcacc
    14041 attatagtta atatcaaaat cttggaacgt gttatttgta ggttgaacat ttaacttcgc
    14101 agcattatca gctgttgaac ttgatgtata cgttgtcgca acaccataac tatctgttgt
    14161 tacaaatgca ccaaacgcac ctccaccagc tacattagat gggtcagcat tcgcctttgc
    14221 agctgaattt ggtttagatg tattgtgata cgtatccaat ttgaagccaa atgcgttact
    14281 taagccacca atacctactg cggcaccgtt taaccctgtt tcacctaata cacctggtga
    14341 aaaggcaaaa ccgataccat ctccaccatt tccatgccct tcatatttgt tacctaaatt
    14401 tacttttcca gaaaaatgaa aactcttatt agagtcaata cgtgttccta atgtaatagc
    14461 acctttttgg ctgtatgcat cctgtgttaa cgtcacaata ccggtacttt gatcataggt
    14521 agcattacct gacgttgtca tatattgttt taagttatct ttattaactg taattgtatt
    14581 agcagttact gcggttgtcg tcgctgctga cgcaaatgtt gacatagcta agcgactgaa
    14641 agttcgaagt tttactggtg cggtgctagt tgacgttgtg ctagttttgt ttaagttgac
    14701 cgaagatggc gttgtgcttt gtgaagtgtt atttgatgca gtactttgat ttgttgatgt
    14761 attaattggt tgttctgtac ttgaagttga agctacagat ttagtatcag aacttgatgt
    14821 agtattcttt gaggatgttg attctgatgt agatgtcaat ttctcttgtt gattgcttgt
    14881 actattagtt gtcgaagtga ccttttcaga cttttcactt gagactgtgt cactatttga
    14941 tgtttgtacc gaactactat ttttcgttac acttgtggaa tcggctgttg atgttgatgc
    15001 ttcgattgtc gttgagtttt gattacctac tgtttcactt tgtgtgttta attcagaagt
    15061 taatggtgca tcagaagccg caaaagcttg ctggtcatgc aacatattta ccgtgaatgc
    15121 accaccaata accgccgtag ttttcagtcc gtatcccgtc atttttttac taatgctttg
    15181 attatcttga ctcactaaac tatgactaat aaatggtagc cccataattt tgaacatttc
    15241 tatttcttta attccggatt ttacccaatt ttttccagat ttataaagtc ttactcttgt
    15301 tttttcgttt gctaagctgt catgaaatgc tttctgtctt ttactcatgt aataactcct
    15361 tgtattatct ttacattcat tagattataa tatatgccac tattcaattt aatacaactc
    15421 ttttttgata caaaaatact cattttgtta aaatttgtaa aaattcattt ttattcgtct
    15481 aaatgtaatc gttttcatat ttttaaaatt actttttctc gtttatgcgt ataatctttt
    15541 tttatataaa tttggctaat tggctttatg tttaatcatt ataattgttt cgtttttaaa
    15601 ataattattg tattaatata tctataccat ccacctttta tttataaata gttaatttac
    15661 aactaaacga taaatattat atgcaaaata catctttaat attaaagtaa taccaatatt
    15721 ttttcaataa acctagtgta atatatgtgt aattctaaaa gattcttctt taaaaatata
    15781 aataccacga catattgctt taacattttc atttataaag cgaaaaaatg catcgctact
    15841 aagttgaatg tttagtaagg atgcatttaa ttcactaaaa tgattaaatt acttatatct
    15901 tttcatctga ttgattatcg aaatttcttc cttctaaacc tgctaactct tctttagaag
    15961 ctgcaggtgc tttcatttca aatatctcat tcactactgt gtaatcgtaa tatcctaatc
    16021 tggcaatagg tttaatcgac ttaatgtcca atttaccatt atcaagaata accttatcgt
    16081 caatatgaac ttgggcaact cttcctataa caatatctac ggtagatact ggatctccag
    16141 ttggaatacg aatcgtttga acgtactcac attcaaaatg aactggcgat tcttttacac
    16201 gatatcctgg agcttctata catttttcct ttgttacatc tgcaaaatta aattcatcct
    16261 cttctggtgg caatgctttc gatgataaat taactgcttc tcttaaatca tacgttgcca
    16321 tattccacac aaaccaacct gtctcttcag catttttcac tgtatcttta cgttcgtgat
    16381 caccaagaac ggattgattt gctgcgaaca taaccatagg cggatcccaa gttaagtttt
    16441 gatactgact ataaggcgct aaattatctt tcccatcttt cgatacagta gagatccacc
    16501 ctattggacg tggtactgta ctacttttaa atgggtcgtg cggtaaacca tgacttctta
    16561 caccttgttt tggcgaataa ttcatactat cttcacccct tataagtaat tacatttaag
    16621 gttacgccct ctttacataa gcgtctaata taaataaaca atttatttat aagtagaaac
    16681 tatatatgac gtggttgctt ataatttgcg ttcttgattc gaaaaattca gataaggatt
    16741 tatacaatta atatttatga tatcttttgt aaatttaatt aattatagtt acttcaatca
    16801 tgattagttt ataataataa agtgaaattg aaaaagacag ctattatgcg atgagcgaaa
    16861 aacttcaagt aaaacaagaa tttaacggcg ttacaaggca atacttgaat ttcagaacag
    16921 atagtatcga tgatctggat aagttcaaaa aagaattcaa acctgttatg cacttaaaag
    16981 gtgatgcatt caatcaacag ttacaatctt tgattaacaa atatccacaa atacaaaaaa
    17041 atatgaaatc agagttcatt gcttattatg ataaagaaaa aaatagagaa acagtaaaaa
    17101 actatgcttg gaaccttcaa aaatctataa atgacattat gcaatcatat cctagcacaa
    17161 aatttgtaca gttttataaa gatgatgttt ccccgtcaat ggtagatgga aatggccgtt
    17221 taaaatcgga tactaatgta atttccatcg agggaggaaa gtatgatgag aacaaatgat
    17281 catataaccg atttagatgc tattgagtta agtagcggtt ggaattacaa ttcgaaattg
    17341 gagactggca aaaatgcact actatcagat agccttttaa gagatattaa agtattaagt
    17401 tctaatgcta aaggcgacgc tgacatacta gaaacagaac ttcgtgctgt aataaactca
    17461 atgtctcaaa acgaaggtaa atctgttgat gcacttaaaa atagtaccgc ttctctaaaa
    17521 aacaatctaa ccaaattaaa agagcaactc gacaaaacta cacattaatt tcatacattt
    17581 tgagtgaaca gaattatatt tccatagcaa acattcctaa actacacata cgttacataa
    17641 ttgattcatt tttatagaaa cgggtaaaaa tgataaagta atcatctttc acaaaaaatc
    17701 aaatattggg aggaattaaa tatgattttg cgttacgcaa cgaatttaaa acttgcgaaa
    17761 gagctatacc aagccagtaa acctaaattg caagaagata agggtatgat tgaactgttt
    17821 gaacatactt ttggcttgca aaaagaactt gtaaaatacg tcggcattgc agaagcaact
    17881 actgctgctt tatactcagc tagctttatc aataaaaaca tttctcgcct tgcatcacta
    17941 tcaacgatag gcattttaag tgtagctgct tacaaacatt tcgaagctgg ccaaggtaaa
    18001 aaaggtgcac aacatgcatt aaatttatta ggtcttgcta ctttaagttt actagataca
    18061 ttttcttgta ataaaaaata gtcttcattt tagaagcaca tactttagca cctgtgtact
    18121 tttgattcaa ttcataaacg gtataccgct atagcatgtg ttttattatt tttctggtct
    18181 ctttatcgta aatattcccc ctaatttagc ataatcattt ccggctaaac gagaaacagc
    18241 ctgtaatgcg tgtggattga ttttaaaatc accctcataa atactgtcat caatatgata
    18301 agttacaatt tcacctatta ttaaatcagc cccatctaat acatctccaa gcaatatcat
    18361 ttgcgatagt ttacattcga atctcatttt cgcatcttta attcctggcg tcttaatcgt
    18421 tgtagatgtt aaaagtgata attctgtacg actcaactca ctgtcaccat atgctaacgg
    18481 cgctgcagtc tcattaatat cttgaacatt atcttcgtct gtaatatgca caacaaagtc
    18541 tccagtccgt tctatattta atgcagtatc ttttctctta cctcctgcac gttgaactgc
    18601 aatagcaatc attggcggat gattattaac aatattaaaa aagctaaatg gtgctgcatt
    18661 tactgatgca tcttgattta atgttgtaac aaaagctata ggtcgtggaa taattgaacc
    18721 aattaataat ttatagtttt ctctagcagt taatgattgt gcatcaaacg tatacataat
    18781 acctacctct tttctaagta tatctaggta tttctccgat tttggttaat ttaaacatct
    18841 attctcctct gaaaatcact tgtatttatt tagcaaatct tttgaaatat gacacatatg
    18901 catatcttct ggatattttt ctaaatgttg ctgatgttct tcagcacttt taatgtagtt
    18961 agacagcggt aagacttcca ctgcaatttg atctctgtct ttacgtcgtt caatgaactg
    19021 acgcgcttca attaagtggt catctacaca actatataaa cccgttcgat acttttgtcc
    19081 aatatcattt ccttgttgat tcacactgta aggatcaatg atttcaaata aataattcat
    19141 aatgtctgta attgttaaca tacgatcatc gaaatgaagt ttgacacatt cagcataacc
    19201 atcatacgga ccgtctaatt tagagcttct tccatttgct cttcctgctt ctgtatgtat
    19261 aattccaggt attgttgcaa aaaatgcttc aacaccccat aaacatcctc ctgctacata
    19321 aacaactgcc atatttacac ctcatcatcc ttttttatat ttttaacaag gttataccat
    19381 ttaataccgc catgacatga ttctgataca ccttcattac gatacccata tttttcataa
    19441 aatgaaatta atgattctcg acatgttaac gttacaccat gtcgatgatg attcttagca
    19501 agagtttcaa aatagtttag taagcgacct gcaataccct gaccttgata atttggtgct
    19561 acaacaagac ctaacacact aatatagcca ccttcactat tatttgtgga gacattttta
    19621 aataaatcat cgctaatgta acgctctttt atgactggac cgttgatgta tccaattact
    19681 tcatcattct tttcagctac aataaaagtt tcttgaattt gttcaattct caatttcaat
    19741 gcctctcgtg ttgcagcttc ttccggtgaa aaaccaatat tttcaagtgc aataatttga
    19801 tctaagtcct gcatgattac ttttcttaat accataaaaa cacttcctta gccctatata
    19861 tcaaaagtaa taccttcatc tttatattca ttattttcaa actaccttca ctataaaaaa
    19921 cgagtctttt tattgaaagc cattcgcctt aatcaattag acaagttgta ttatacgaat
    19981 tagtaaataa tcatgattat aattcatttt aagcatactc aaaaagactg gtacatgtat
    20041 accagtctaa ttcgaaaaaa tatatttagt taaaaccatt tcaaaaacga cttcactttt
    20101 cctggcttag tcggtctgtc taaataaaca aaattatcat tgattactga actggcattt
    20161 cgaagtaatg cttcaatatc attcgaatat ttcttcaatt tatgattgtg aaataattct
    20221 tgcatcaaaa atggtctttg gtcacatgaa tgtgcatctg aagctacaaa atgagccaaa
    20281 ttacattcta taaattgtaa tgataacttt tgaatgtttt taccaaatcc accaactaaa
    20341 gaactcgatg ttaattgact cagtgcccca tttgcaacca attcatataa tatttccgga
    20401 tttttggcga tacttctatt tctttcagga tgtgcaatga ttggtatgta acctctcgat
    20461 tgtatttcaa aaaacaattg ttttgtataa tgtggtactt cgcccgttgg aaattcaatt
    20521 aataaatatt tcgaacgatt aataccttga atactaccat tatctaagcc tttcagaatc
    20581 gaatctgtaa ttctaatttc ttgcccggga aataatttaa tatccaatgc ttgaacttct
    20641 ggatgcgttc ttaactccgc caatttcaca agcacttgtt gaaatgtatt atcatatctc
    20701 ggatgcaaat gatgaggtgt cgctacaata cttgttacac cttcatcctt agcttgcttt
    20761 aatagtgcaa tactcttttc aattgtttta ggaccatcat ctatatcaac taatatatgg
    20821 ttatgaatat caatcatgat tcatcagtcc cataatatgc atagtaacta gcacttttat
    20881 ctttaggcat tctatttaag actacaccta ataatttagc acctgttgct tcaataagtt
    20941 cttttccttt tttaacttca tctctattat tattttccga attaactacg tagacaacat
    21001 tgccggtaaa ctttgaaaat aattgcgcat ctgtaactgt gttcactggt ggcgtatcga
    21061 taattacaaa gttataattc atcaataatg tgtcatacaa atttgcaaat gcccttgatg
    21121 taattaactc tgacggattc ggtgggattg gcccagacgt caagacgtct aaatcttgaa
    21181 tttcagttga gataatactg tcttgataag ttgaccaatt tagcaataaa cttgataggc
    21241 cttcattgtt tggcaaatta aaaatataat gctgcgtagg tttacgcata tccccgtcta
    21301 cgattagtgt tttataacct gcttgcgcat atgcaactgc taaatttgct gcaattgtag
    21361 acttacctgc gcctggtgcc tctgaagtga ttacaatgct ttgaactgca ctgtcaggat
    21421 ttgcaaacat aatatttgat cttatgcctc gaaatttctc gctaataggt gactttggtt
    21481 gttcatggac aattaaactt gatgtacttc ttcgtgtatt cgtcatggta attcctcgta
    21541 aattaaaatt tttgtattga acctaaaata ggtaatccta gttgcgattc aacatcttct
    21601 tctgtcttaa tacgcttatc taataattct tttaagaaaa taatcaatat tgctaaaaca
    21661 ataccaacaa taatgctgat aactaagttg acagatacta ttggagatac ttttacagca
    21721 ttatcatgtg ctgaggaaag tatcgtaaca ttatcaacac tcataatttt aggcatgtca
    21781 tgagcaaaaa ctttagatat tttattaaca attttgtcag attcagattt attcccagtg
    21841 gtaactgata cagtaataat ttgagagttt gtttgattgg ttacttttaa aaatgaattc
    21901 aactcagctg ttgaatactg accatcaaat tctctagata ctttatctag aattctagga
    21961 cttttgataa tttccgtata tgtattaaca gactgcaaac tactttgaac attttggaaa
    22021 gctaaatcac ttgaggactt tttcatgttc actaatattt gagtagaagc agtatatttg
    22081 tcaggcataa caaaaaaggt taatgccgca cttactacaa gacatattgc cggtaaaata
    22141 agcaataatt taatattctt ctttagaata tttaatagtt ttactaaatc aaacttttct
    22201 ttcatggttt cctccacata atcaatcatt gtattcatta tgtatgtttt ataaatcgga
    22261 caattatatc tagtttaacg accacaaaac atacacaact acattttctc taattattta
    22321 tataaatatt ttatcgttta aaattatatc atgattctct accattatgt ataacttatt
    22381 tatatttttg cacaagatat aatattgtcc aactttaaat atccaaacct attaataata
    22441 aaactagata ccatcgtact ctatcatggc tttcttataa tcgagtagaa gcatcatcat
    22501 tacttgatta tttgctcttt acaacaccga gcgtgcccgt actcggtaat tcaatacctt
    22561 gcgtaacccg tcactgtgag ttgggttaat gataataaag cccacacctt ttaaaaagat
    22621 gtgggtaatt tatataattt ttatttacat ttttaactta taaaaaagcg cctatgtcat
    22681 gatttaccat cacataggcg cttatcaata aattattact tattactttc catttcatct
    22741 aatttatgcg gatttcctgt aattagatga caacttattc ttttcagggg aacattacac
    22801 ttttataata tgttcaaaga caaacttaac cattcacaaa tataaagaat aatattatca
    22861 aatcattgaa caaatcgtat tttgcaacaa ttgatattta tattaatgta ttgcatttaa
    22921 tttataaaat tcatatacat cttaatattc tcaatatcga tttgtattgt caactttata
    22981 tagatttaaa aaaataatct catgtctttt tttacaaaag taagttaatt attacaaact
    23041 agtaacaaaa attatttctt caaaaatata tttagtagcg aatacacttc atctttgaat
    23101 tgacttttac tttcttccac tgctccaaat ttttgcgaaa aggatgcttt caaataccaa
    23161 ctttcaagaa acagcaatat taaattctga aagtcttctt ttgtcatctt tatctttgat
    23221 tcatcataga attttgctat ctctttactt aatgattgat ttaaatcttg tatttgtccg
    23281 taaatatttc cagaaaattc ctcaggcgta ttagataatt gaacgtacat tctaatatac
    23341 ctttcttcga tgtcgaaaat aaactcaaat aagaattgat ataaagcatc aattgaatag
    23401 ttcgatttat tttgattcat cataataata ttattaaggt aatcaaaaca acatttaaca
    23461 ctttgttcgt aaatactttt tttcgagtca aaatggtaat ataaactcgc tttctttata
    23521 tttacacttt tagctatatc atcaagtgtt gtaccgtcat accccttctc tgaaaataag
    23581 gttattgcgt tatcaataat cttatccttc aatttttata accccctact gaaaattaat
    23641 cacactatgt tacaggaaaa ttaagttgca attacaaata tttccgttta attataacaa
    23701 caatctattg caaattaaaa tactatcaat taccatatgg cttacaacct aactaacgaa
    23761 aggtaggtaa agaaattgca attttttaac tttttgcttt tttatcctgt atttatgtct
    23821 atttactgga ttgtcggttc aatttatttc tattttacca gagaaattag atattcattg
    23881 aacaagaagc ctgacataaa tgtggatgaa ttagaaggca ttacattttt acttgcctgt
    23941 tataacgaaa gtgaaacgat tgaagatacg ttgtctaatg ttcttgcact caaatacgag
    24001 aagaaagaaa ttattatcat taatgatgga agttcagata atacagcaga actcatctat
    24061 aaaatcgacg aaaataatga ctttattttc gtcgatttac aagaaaacag aggtaaagcc
    24121 aacgcactca atcaaggcat taaacaggct tcatatgatt atgtaatgtg cttggatgca
    24181 gatactatcg ttgatcaaga tgcaccatat tatatgattg agaatttcaa acatgatcca
    24241 aaacttggtg cagttacagg taatcctaga attcgaaata agagttctat tttaggtaaa
    24301 attcaaacga tagaatatgc aagtttaatt ggctgtatta agcgaagtca gacacttgct
    24361 ggcgcagtca atactatttc gggtgtcttc actctattta aaaaaagtgc agttgtcgac
    24421 gttggctact gggatactga tatgattacc gaagatattg cagtttcttg gaaattgcat
    24481 ttacgtggat atcgtattaa gtatgaaccg cttgccatgt gttggatgtt ggttccagaa
    24541 acattgggag gtctttggaa gcaacgcgtg agatgggctc aagggggaca cgaagtatta
    24601 ctacgagact tttttagcac aatgaaaacg aaaaggtttc ctttatatat tttgatgttt
    24661 gagcaaatca t
//
LOCUS       DANRDI010000024        24312 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.024, whole genome shotgun sequence.
ACCESSION   DANRDI010000024 DANRDI010000000
VERSION     DANRDI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.024"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            330..2939
                     /gene="adhE"
                     /locus_tag="PZ377_000749"
                     /gene_synonym="adhC"
     CDS             330..2939
                     /gene="adhE"
                     /locus_tag="PZ377_000749"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDM6357636.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            3284..3952
                     /gene="capA"
                     /locus_tag="PZ377_000750"
     CDS             3284..3952
                     /gene="capA"
                     /locus_tag="PZ377_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDM6357637.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            3968..4654
                     /gene="cap8B"
                     /locus_tag="PZ377_000751"
     CDS             3968..4654
                     /gene="cap8B"
                     /locus_tag="PZ377_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDM6357638.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            4657..5421
                     /gene="cap8C"
                     /locus_tag="PZ377_000752"
     CDS             4657..5421
                     /gene="cap8C"
                     /locus_tag="PZ377_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDM6357639.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            5441..7265
                     /gene="cap8D"
                     /locus_tag="PZ377_000753"
                     /pseudo
     CDS             5441..7265
                     /gene="cap8D"
                     /locus_tag="PZ377_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            7255..8283
                     /gene="cap8E"
                     /locus_tag="PZ377_000754"
     CDS             7255..8283
                     /gene="cap8E"
                     /locus_tag="PZ377_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDM6357640.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            8296..9405
                     /gene="cap8F"
                     /locus_tag="PZ377_000755"
     CDS             8296..9405
                     /gene="cap8F"
                     /locus_tag="PZ377_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDM6357641.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            9409..10533
                     /gene="cap8G"
                     /locus_tag="PZ377_000756"
     CDS             9409..10533
                     /gene="cap8G"
                     /locus_tag="PZ377_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDM6357642.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            10536..11162
                     /locus_tag="PZ377_000757"
     CDS             10536..11162
                     /locus_tag="PZ377_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HDM6357643.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            11167..12276
                     /locus_tag="PZ377_000758"
     CDS             11167..12276
                     /locus_tag="PZ377_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDM6357644.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            12290..13456
                     /locus_tag="PZ377_000759"
     CDS             12290..13456
                     /locus_tag="PZ377_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDM6357645.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            13449..14654
                     /locus_tag="PZ377_000760"
     CDS             13449..14654
                     /locus_tag="PZ377_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDM6357646.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            14655..15860
                     /gene="cap8L"
                     /locus_tag="PZ377_000761"
     CDS             14655..15860
                     /gene="cap8L"
                     /locus_tag="PZ377_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDM6357647.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            15871..16428
                     /gene="cap8M"
                     /locus_tag="PZ377_000762"
     CDS             15871..16428
                     /gene="cap8M"
                     /locus_tag="PZ377_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDM6357648.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            16428..17315
                     /gene="capN"
                     /locus_tag="PZ377_000763"
     CDS             16428..17315
                     /gene="capN"
                     /locus_tag="PZ377_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDM6357649.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            17369..18631
                     /gene="cap8O"
                     /locus_tag="PZ377_000764"
     CDS             17369..18631
                     /gene="cap8O"
                     /locus_tag="PZ377_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDM6357650.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            18708..19853
                     /gene="cap8P"
                     /locus_tag="PZ377_000765"
     CDS             18708..19853
                     /gene="cap8P"
                     /locus_tag="PZ377_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDM6357651.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(19918..20244)
                     /gene="isdI"
                     /locus_tag="PZ377_000766"
     CDS             complement(19918..20244)
                     /gene="isdI"
                     /locus_tag="PZ377_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDM6357652.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(20251..20634)
                     /locus_tag="PZ377_000767"
     CDS             complement(20251..20634)
                     /locus_tag="PZ377_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDM6357653.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            21061..22548
                     /locus_tag="PZ377_000768"
     CDS             21061..22548
                     /locus_tag="PZ377_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDM6357654.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            23194..24153
                     /locus_tag="PZ377_000769"
     CDS             23194..24153
                     /locus_tag="PZ377_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDM6357655.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
BASE COUNT         8694 a         3439 c         4539 g         7640 t
ORIGIN      
        1 atttgtaaca tctgttgata ggtatcgaca aggacctttt aatgttgtgt tttgtgcgat
       61 ataacaagct ttttaggcta ttaaagaata ttcagaatta aatatataat tatgaataaa
      121 ttatgtcatt gaaaaaatta tgattataaa atcattctaa aaacaccgaa ataacaatga
      181 tttcatgaaa acatttattt taaaatttga tatttgttca aataatattc gaaattaaac
      241 ttttttgtat agaattttct ttatatcctg agagacatgt actataatgt ttgtgaaata
      301 attcacaaag tataaaggag tggttgtata tgttaactat acctgaaaaa gaaaatcgtg
      361 gatcgaaaga acaagaagtg gcaattatga ttgatgctct agctgacaaa gggaaaaaag
      421 cattagaagc attatctaaa aagtcacaag aagaaattga tcatattgtt catcaaatga
      481 gcttagcagc tgttgatcaa catatggtgc tagcaaaatt agcacatgaa gaaactggaa
      541 gaggtatata cgaagataaa gcgattaaaa atttatacgc ttctgaatat atatggaatt
      601 caataaaaga caataagaca gtagggatta ttggtgaaga taaagaaaaa ggattaacgt
      661 atgtagcgga accaattggt gttatttgtg gtgttacgcc aacaacaaat cctacgtcga
      721 caactatttt taaagcgatg attgcaatta agacaggaaa tccaatcatt tttgcattcc
      781 atccaagtgc acaagaatcg tcgaagcgtg cagcagaagt tgtattagaa gcggcaatga
      841 aggcaggtgc acctaaagat attattcagt ggattgaagt gccttctatc gaagcaacaa
      901 aacaattaat gaatcacaaa ggtattgcat tagttctagc aacaggtggt tcgggcatgg
      961 ttaagtctgc atattcaact ggcaaaccgg cattaggtgt gggaccaggt aacgtgccgt
     1021 cttacattga aaaaacagca cacattaaac gtgcagtaaa tgatatcatt ggttcaaaaa
     1081 catttgataa tggtatgatt tgtgcttctg aacaagttgt agtcattgat aaagaaattt
     1141 ataaagatgt tactaatgaa tttaaagcac atcaagcata ctttgttaaa aaagatgaat
     1201 tacaacgctt agaaaatgca attatgaatg aacaaaaaac aggtattaag cctgatattg
     1261 tcggtaaatc tgcagttgaa atagctgaat tagcaggtat acctgtcccc gaaaatacaa
     1321 aacttatcat agccgaaatt agcggtgtag gttcagacta tccgttatct cgtgaaaaat
     1381 tatctccagt attagcctta gtaaaagccc aatctacaaa acaagcattt caaatttgtg
     1441 aagacacact acattttggt ggattaggac acacagccgt tatccataca gaagatgaaa
     1501 cattacaaaa agattttgga ctaagaatga aagcttgtcg tgtacttgta aatacaccat
     1561 cagcggttgg aggtattggt gatatgtata acgaattgat tccgtcttta acattaggtt
     1621 gtggttccta cggtagaaac tcaatttcac ataatgttag tgcgacagat ttattaaaca
     1681 ttaaaacgat tgctaaacga cgtaataata ctcaaatttt caaggtgcct gctcaaattt
     1741 attttgaaga aaatgcaatc atgagtctaa caacaatgga caagattgaa aaagtgatga
     1801 ttgtctgtga ccctggtatg gtagaattcg gttatacaaa aacagttgag aatgtattaa
     1861 gacaaaaaac ggaacagcct caaattaaaa tatttagcga agtcgaaccg aacccatcaa
     1921 ctaatacagt atataaaggt ctggaaatga tggttgattt ccaaccggat acaatcattg
     1981 cacttggtgg tggttcagcg atggatgctg caaaagcaat gtggatgttc tttgaacacc
     2041 ctgagacatc attcttcggt gctaaacaaa agttcctaga catcggtaaa cgtacttata
     2101 aaataggcat gcctgaaaat gcgacgttca tttgtatccc tacgacatca ggtacaggtt
     2161 cagaagtaac accatttgca gttatcacag atagtgaaac aaatgtaaaa tatccgttgg
     2221 ctgattttgc tttaacacct gacgttgcaa ttattgaccc tcaatttgtg atgagtgtgc
     2281 caaaaagcgt tacagcagat acaggaatgg atgtactaac gcatgcaatg gaatcatatg
     2341 tatctgtaat ggcttcagac tacacaagag gtttgagtct acaagcgatt aaattgacgt
     2401 tcgaatattt aaaatcatct gttgaaaagg gtgataaagt ttcaagagag aaaatgcata
     2461 acgcatcaac tttggctggt atggcatttg caaatgcatt cttaggcatt gcacactcaa
     2521 ttgcgcataa aattggtggc gaatatggta ttccgcatgg tagagcgaat gcgatattac
     2581 taccgcatat tatccgttat aatgccaaag acccgcaaaa acatgcatta ttccctaaat
     2641 atgagttctt cagagcagat acagattatg cagatattgc caaattctta ggattaaaag
     2701 gtaatacgac agaagcactc gtagaatcat tagctaaagc tgtctacgaa ttaggtcaat
     2761 cagtcggaat tgaaatgaat ttgaaatcac aaggtgtgtc tgaagaagaa ttaaatgagt
     2821 caattgatag aatggcagag ctcgcatttg aagatcaatg tacaactgct aatcctaaag
     2881 aagcactaat cagtgaaatc aaagatatca ttcaaacatc atatgattat aagcaataat
     2941 ctatctgata ataatcatct aactcacctg aaattacaaa agtaaaaaat gccacataaa
     3001 ctttaagtcg ataatcatta tacggttatc ggcttttatt tattgccaaa tcttcagaga
     3061 gatacaaact agacaatcat ttttttaaat aaagaaaata ttaagattga tactcatttc
     3121 acaaactatt actactttag agtataatta tttttaattt catataaata aaaaggcgaa
     3181 aataatgcgg tttaaaagta attaattgtt caaacgatat gtaatatgta aatactatat
     3241 atataatacc aattttaatg aaaattttta agggaggtaa ataatggaaa gtacattaga
     3301 attaacaaaa attaaagaag tattacaaaa aaacttgaag attttaatta ttttaccgct
     3361 attattttta attattagcg ctattgttac atttttcgtc ttatcaccta aatatcaagc
     3421 taatactcaa attttagtga atcaaactaa gggtgacaat cctcagttta tggcgcaaga
     3481 ggttcaaagt aatattcaac ttgtaaatac gtataaagaa attgttaaaa gtcctagaat
     3541 tttagatgag gtgtcaaagg acttaaatga taagtattca ccatctaaat tgtcgagtat
     3601 gttgacaatt acaaaccaag aaaatacgca acttatcaac atccaagtta aaagtggtca
     3661 taaacaagat tcggaaaaaa ttgcgaatag cttcgctaaa gttacaagta aacaaattcc
     3721 gaagattatg agtgtggata acgtatcaat tttatctaaa gcagacggta cagcagttaa
     3781 agtcgcacca aaaactgtag tgaatctaat cggtgcattc tttttaggat tagttgtcgc
     3841 gcttatatat atcttcttca aagtaatttt cgataagcga attaaagatg aagaagatgt
     3901 agagaaagaa ttaggattgc ctgtattggg ttcaattcaa aaatttaatt aaggatggtt
     3961 gctacttatg tcaaaaaagg aaaatacgac aacaacacta tttgtatatg aaaaaccaaa
     4021 atcaacaatt agtgaaaagt ttcgaggtat acgttcaaac atcatgtttt caaaagcaaa
     4081 tggtgaagta aagcgcttat tggttacttc tgaaaagcct ggtgcaggta aaagtacagt
     4141 tgtatcgaat gtagcgatta cttatgcaca agcaggctat aagacattag ttattgatgg
     4201 cgatatgcgt aagccaacac aaaactatat ttttaatgag caaaataata atggactatc
     4261 aagcttaatc attggtcgaa cgactatgtc agaagcaatt acgtcgacag aaattgaaaa
     4321 tttagatttg ctaacagctg gccctgtacc tccaaatcca tctgagttaa ttgggtctga
     4381 aaggttcaaa gaattagttg atctgtttaa taaacgttac gacattatta ttgtcgatac
     4441 accgccagtt aatactgtga ctgatgcaca actatatgcg cgtgctatta aagatagtct
     4501 gttagtaatt gatagtgaaa aaaatgataa aaatgaagtt aaaaaagcaa aagcacttat
     4561 ggaaaaagca ggcagtaaca ttctaggtgt cattttgaac aagacaaagg tcgataaatc
     4621 ttctagttat tatcactatt atggagatga ataagtatga ttgatattca taaccatata
     4681 ttgcctaata tcgatgacgg tccgacaaat gaaacagaga tgatggatct tttaaaacaa
     4741 gcgacaacac aaggtgttac agaaatcatt gtaacatcac atcacttaca tcctcgatat
     4801 accacaccta tagaaaaagt gaaatcatgt ttaaaccata ttgaaagctt agaggaagta
     4861 caagcactaa atctaaagtt ttattatggt caggaaataa gaattaccga tcaaatcctt
     4921 aatgatattg atcgaaaagt tattaacggt attaatgatt cacgctattt actaatagaa
     4981 tttccatcaa atgaagttcc acactatact gatcaattat ttttcgaatt acagagtaaa
     5041 ggctttgtac cgattattgc acatccagag cggaataaag caataagtca aaaccttgac
     5101 atactatacg atttaattaa caaaggtgct ttaagtcaag tgacaacggc gtcattagcg
     5161 ggtatttccg gtaaaaaaat tagaaaatta gcaattcaaa tgattgaaaa caatctgaca
     5221 catttcatcg gttcagatgc gcataacaca gaaatcagac cgttcttaat gaaagactta
     5281 tttaatgata agaaattacg tgattattat gaagatatga acggatttat tagtaatgcg
     5341 aagttagttg ttgatgataa aaaaattcct aaacgaatgc cacaacaaga ttataaacag
     5401 aaaagatggt ttgggttata aacagcaaat gaggggtttt atggcacatt tatctgtgaa
     5461 attgcggctt ttaatactag cattaatcga ttcactgata gtgacatttt cagtattcgt
     5521 aagttattac attttagaac cgtatttcaa aacatattct gtcaaattat taatattggc
     5581 agctatatca ctattcatat cgcatcatat ttcagcattt atttttaata tgtatcatcg
     5641 agcgtgggaa tatgccagtg tgagtgaatt gattttaatt gttaaagctg tgacgacatc
     5701 tatcgttatt acgatggtgg tcgtgacaat tgttacaggc aatagaccgt tttttagatt
     5761 gtatttaatt acttggatga tgcacttgat tttaataggt ggctcaaggt tattttggcg
     5821 tatttatcgg aaataccttg gaggtaagtc atttaataag aagccaactt tagttgttgg
     5881 tgctggtcaa gcaggttcaa tgctgattag acaaatgttg aaaagtgacg aaatgaaact
     5941 tgaaccggta ttagcagtcg atgatgacga acataaacgc aatatcacaa ttactgaggg
     6001 tgtaaaagtc caaggtaaaa ttgcggatat tccagaacta gtgaggaaat ataagattaa
     6061 aaaaatcatc attgcaattc caactattgg tcaagagcgt ttgaaagaaa ttaataatat
     6121 ttgccatatg gatggcgttg agttattgaa aatgccaaat atagaagacg tcatgtctgg
     6181 tgagttagaa gtgaaccaac ttaaaaaagt tgaagtagaa gatttactag gcagagatcc
     6241 tgttgaatta gatatggata tgatatcaaa tgaattgacg aataaaacta ttttagttac
     6301 gggtgcaggt ggttcaatag gatcagaaat ttgtagacaa gtttgtaatt tctatccaga
     6361 acgtattatt ctacttggcc atggtgaaaa cagtatttat ttaatcaatc gtgaattgcg
     6421 aaatcgcttc ggaaaaaaat gttgatatcg ttcctattat agcggatgtg caaaatagag
     6481 cgcgtatgtt tgaaattatg gaaacgtata aaccatacgc agtttatcat gcagcagcac
     6541 acaagcacgt gccgttaatg gaagacaacc ctgaagaagc agtacgtaat aatattttag
     6601 gtacgaaaaa tactgctgaa gctgctaaaa atgcagaggt aaagaaattc gttatgattt
     6661 ctacggataa agccgttaat ccgcctaatg tcatgggagc ttcaaagcga attgcagaaa
     6721 tgattattca aagtttaaat gatgaaacgc atcgaacaaa ttttgttgca gtgagatttg
     6781 gtaatgtact tggatcgaga ggatctgtga ttccactttt caaaagtcaa attgaagaag
     6841 gtgggccagt tactgtgaca catcctgaaa tgacacgtta ctttatgaca attcctgaag
     6901 cttctagact agttttgcag gcaggggcat tagcagaagg tggcgaagta tttgtgctag
     6961 atatgggaga accagtgaaa attgtagatt tggcacgtaa tttaattaag ctaagtggta
     7021 aaaaagaaga cgacatacgc attacttata cagggattag acccggcgaa aaaatgtttg
     7081 aagagcttat gaataaagat gaggttcatc ctgaacaagt atttgaaaaa atttatcgtg
     7141 gcaaagtaca acatatgaaa tgtaatgaag ttgaagcgat tattcaagac atcgtcaatg
     7201 actttagtaa agaaaaaatt attaactatg ccaatggcaa aaagggagat aattatgttc
     7261 gatgacaaaa ttttattaat tactgggggc acaggatcat tcggtaatgc tgttatgaaa
     7321 cagtttttag attctaatat taaagaaatt cgtatttttt cacgcgatga gaaaaaacaa
     7381 gatgacattc gaaaaaaata taataattca aaattaaagt tctacattgg tgatgtgcgt
     7441 gatagtcaaa gtgtagaaac agcaatgcga gatgtttatt acgtattcca tgcagcagct
     7501 ttaaaacaag tgccgtcatg tgaattcttt ccagttgagg cagtgaagac aaatattatt
     7561 ggtacagaaa atgtcttaca aagtgctatt catcaaaatg ttaaaaaagt catatgttta
     7621 tctacagata aggcagcgta tcctattaat gctatgggta tttcaaaagc aatgatggaa
     7681 aaagtattcg tagccaaatc aagaaatatt cgtagtgaac aaacgcttat ttgtggtaca
     7741 agatacggta atgtgatggc ttcaagagga tcagtaatac ctttgtttat cgacaaaatc
     7801 aaagctggag aacctttaac gattacagat cctgatatga caagattttt aatgagctta
     7861 gaagatgcgg tagaactagt tgttcatgca tttaagcatg cagagacagg agatattatg
     7921 gttcaaaaag caccaagctc aacggtaggg gatcttgcga ccgcattatt agaattgttt
     7981 gaagctgata atgcaattga aatcattggt acgcgacatg gagagaaaaa agcagaaaca
     8041 ttgttgacga gagaagaata cgcacaatgt gaagatatgg gtgattattt tagagtgccg
     8101 gcagactcca gagatttaaa ttatagtaat tatgttgaaa ccggtaacga aaagattacg
     8161 caatcttatg aatataactc cgataataca catattttaa cggtggaaga gataaaagaa
     8221 aaacttttaa cactagaata tgttagaaac gaattgaatg attataaagc ttcaatgaga
     8281 taggagagat tgacgttgaa tattgtaatt acaggagcaa aaggttttgt aggaaaaaac
     8341 ttgaaagcag atttaacttc aacgacagat catcatattt tcgaagtaca tcgacaaact
     8401 aaagaggaag aattagagtc agcattgttg aaagcagact ttgtcgtgca tttagcgggt
     8461 gttaatcgac ctgaacatga caaagaattc agcttaggaa acgtgagtta tttagatcat
     8521 gtacttgata tattaactag aaatacgaaa aagccagcga tattattatc gtcttcaata
     8581 caagcaacac aagataatcc ttatggtgag agtaagttgc aaggggaaca gctattaaga
     8641 gagtatgccg aagagtatgg caatacggtt tatatttatc gctggccaaa tttattcggc
     8701 aagtggtgta agccgaatta taactcagtg atagcaacat tttgttacaa aattgcacgt
     8761 aacgaagaga ttcaagttaa tgatcggaat gttgaactaa cgctaaacta cgtggatgat
     8821 atcgtcgctg aaataaagcg tgctattgaa ggaactccaa cgattgaaaa tggtgtacct
     8881 acagtaccaa acgtatttaa agtgacattg ggagaaattg tagatttatt atacaagttc
     8941 aaacagtcac gtctcgatcg aacattgccg aaattagata acttgtttga aaaagatttg
     9001 tatagtacgt atttaagcta tctacctagt acagacttta gttatccctt acttatgaat
     9061 gtggatgata ggggttcttt tacagaattt ataaaaacac cggatcgtgg tcaagtttct
     9121 gtaaatattt ctaaaccagg tattactaaa ggtaatcact ggcatcatac taaaaacgaa
     9181 aaatttctag tcgtatcagg taaaggggta attcgtttta gacatgttaa tgatgatgaa
     9241 atcattgaat attatgtttc tggcgacaaa ttagaagttg tagacatacc agtaggatac
     9301 acacataata ttgaaaattt aggcgacaca gatatggtaa ctattatgtg ggtgaatgaa
     9361 atgtttgatc caaatcagcc agatacgtat ttcttggagg tatagcgcat ggaaaaactg
     9421 aaattaatga caatagttgg tacaaggcct gaaatcattc gtttatcatc aacgattaaa
     9481 gcatgtgatc aatattttaa tcagatatta gtacacactg gtcaaaatta tgattataca
     9541 ttgaatcaaa ttttctttga tgatttggaa ttaagacaac cggaccacta cttagaggca
     9601 gttggaagta accttggaga aacgatgggg aatattattg cgaagacata tgatgtttta
     9661 ttacgcgaac aaccagatgc acttttaatt cttggtgata caaatagttg tttagcagca
     9721 gtatctgcta aacgattaaa gattcctgtg ttccacatgg aagcgggtaa tagatgcttt
     9781 gatcagaatg tacctgaaga aatcaatcgt aaaattgttg accatgtcag tgatgtgaat
     9841 ctaccttata cggaacatag cagacgttat ttattagatg aaggcctcaa taaagcgaat
     9901 atctttgtga caggatcacc gatgacagaa gtgatagaag cgcatcgaga taaaattaat
     9961 cacagtgacg ttttaaataa actaggatta gaaccgcaac aatacatttt agtatctgcg
    10021 catagagaag agaatatcga taatgaaaag aattttaaat cattaatgaa tgcgataaat
    10081 gatattgcca aaaagtataa aatgcctgtg atttattcaa cgcatccaag aagttggaag
    10141 aaaattgaag aaagtaaatt tgaatttgat ccattagtta aacagttaaa gccatttggt
    10201 ttctttgatt ataatgcatt gcaaaaagat gcatttgttg tgctatcaga tagtggaaca
    10261 ttgtcagaag agtcgtctat tttgaagttc cctggtgtcc ttattcgaac ttccacagaa
    10321 agaccggaag tactagataa aggtacggtt attgtaggtg gtattaccta taacaatcta
    10381 atccaatccg ttgaactagc aagagagatg caaaacaata acgaaccgat gattgatgct
    10441 attgattata aagacactaa cgtttcgaca aaggtagtta aaattattca aagctataaa
    10501 gatattatca atcgaaatac ttggaggaaa tgacgatgag gatagcgatt gaaaagataa
    10561 ttggtttgct gaaaaaccag tcctctaaag aatcgaatgt taagattcat cgcttggcgt
    10621 atattacaaa ctcaaaattt gatggcaata actatataga tagatggtgt aaaatcagga
    10681 attctcacat tggtgaatac agttatattg gatttggtag tgattttaat aatgtagaag
    10741 taggaagata ttgttcgata tcttcggatg taaaaattgg gttaggaaaa catcctacac
    10801 acttttttag ctcatcaccg attttttatt ctaataataa tccatttaac ataaagcaaa
    10861 agtttataga ctttaatgac caaccaagcc gtacaacaat taaaaatgat gtgtggattg
    10921 gtgcaaatgt aattattatg gatggtttaa caataaatac tggtgcagtc atagcagccg
    10981 gctcagttgt tactaaaaat gtaggagcat atgaggttgt tggtggtgtt cctgcaaaag
    11041 tgattaagaa gcgatttgac aataaaacaa ttgaaaaact tttggaaagc aagtggtggg
    11101 agaaaacgcc tgacaaacta aaaggatttt cggttgaata tttaaataaa aaggatactt
    11161 aatgatatga gaattttaaa tattgtatcg agtaatattg ttcaagaccc aagggtactt
    11221 aaacaaatag aaacaattaa aggcgttacg gatgattata aaattgttgg aatgaataat
    11281 tcacaagcta ctaataagcg attggaaaat ttagattgta attatcgttt gttaggtagc
    11341 aaggtagatc caaaaaatat tctttctaaa ttaattaagc gtataagatt tgcaacaggt
    11401 gttatccgag aaattaaagc ttataaacct gacgtgattc atgcaaatga tttcgacgta
    11461 ttattaatgg tctatttaag caattataaa aaagctaata ttgtttatga tgcgcatgaa
    11521 atatatgcga aaaatgcctt tattaataaa gttccactta tttcaaagtt tgtagaaagt
    11581 atagaaaaac acatagtaaa acatcgtgtt aatgccttcg taacagtaag tcatgcagca
    11641 aaagaatatt atcaatctaa aggatataag aaggaagcga atgttattac gaatgcacct
    11701 attttaaatg atagcagaga atttaaagaa atcgaaaact ttaaagaaat tgtatatcaa
    11761 ggtcaaattg taatggacag aggatatgaa gagtttatta ttgcttcatc agcttttaaa
    11821 caaaatgctc cttcattcat aattcgaggg tttggtccgc atgaagaagt gataaaagaa
    11881 ctgattagtt ataacccgga aaatattagg ttggataaac cagttgaagt aaaagaattg
    11941 gttgataagt tagcagaaag taatgttggt gttgtcttga cgaaaccagt atctattaat
    12001 tttgaatata cagtatctaa taaaattttt gaatgtatac atgctggttt accagtaatt
    12061 ttatctcctg tcaaagagca tatttatctc aatgaaaaat ataaatttgg cattgttcta
    12121 aaggaagtta cgccgttaga aattgaaaag gcggttagaa aattaagaga taatcacgat
    12181 ttgtttaatc atttacgtca aaatgcaatt aaggcgtcta aaattttgaa ttggcaaata
    12241 gagagtgaac ggttagtaga attatataaa ttttaaagag aggtagacta tgaaattttt
    12301 tgtactttgt gcaattatca gcatgaacat atttatagta atctctacat ttactaaaga
    12361 agtattaggg ttccctatag agccggtgta ttactcaacc atggttggta tagcattaat
    12421 tactacggtg tttgctattt ataagataat tgtcacccaa gaaattccgc gagggttaat
    12481 attattaatt gctatatgtt tgctttatct agctttttat tatttttcac cagataagga
    12541 agagaaacta gctaaaaata atattctatt ctttttaaca tgggcagttc cagcggcaat
    12601 tagtggtatt tatattaaat atataaacaa ggctacggta gaaagatttt ttaaattagt
    12661 atttttcata ttttctgttt catttatttt tgtaatttta ataccaaaac ttacaggtga
    12721 gatacctagc tatatcaatt ttggacttat gaactatcaa aacgcttcgt acctttcagc
    12781 atttactgcc ggattaggca tttatttcat tatgaaaggt tcagttaaac ataagtggat
    12841 atatgttcta tttacaataa ttgatatccc tattgtgttt ataccaggag ggcgtggagg
    12901 tgctatttta ttaattcttt acggcttatt tgcatttata cttattacgt ttaaaagagg
    12961 aatacctatc gcagtaaaaa gcattatgta tatttttgca ttaagcatat ctagtgtatt
    13021 gatttacttt ctttttacaa aaggttcgaa tactagaaca ttttcatatc tacaaggtgg
    13081 aacacttaat ttagaaggta cttctggaag aggaccgatt tatgaaaaag gtatttactt
    13141 tattcaacaa agttcgttat taggctatgg gccatttaac tattataaac taatcggaaa
    13201 tataccacat aacatcatta ttgagttgat tctatcattt ggcttattag ggttttttat
    13261 cataatgatt tgcattttgc tactagttta taaaatgatt aggaactatg atccaaacac
    13321 tatagattta ctcgttatgt ttatagcaat ctatccaatc acattattaa tgtttagttc
    13381 aaattattta gttgtaagtg aattttggtt tgtgttgttc tattttatta caaaaggacg
    13441 gcgtcatcat ggctaagaaa gtttttatta tggatagcgt aaagacaata attggtacgt
    13501 tgcttatagc tttaggatta caatttttag cttatccaat tattaatcaa cgagtaggta
    13561 atgaagcgtt cggttctatt ttaacgattt atacaataat aacaatcacg agtgttgtat
    13621 taggcaatac gcttaacaat atacgattga ttaatatgaa tctatacaaa tccaatcatt
    13681 actactggaa atttgcatcg atacttttaa tctcaattct gattgagagt atagctttaa
    13741 ttattgtatt tctttacttt tttaatttga acatcatcga tattatcttt ttaattctac
    13801 ttaatatttt aatgtgttta aggatttatc tgaatgtatt ttttaggatg actttaaaat
    13861 ataatcagat tttgtatatt gctcttattc aatttttagg tttgctgata ggactatttc
    13921 tatattattt aacccaaaac tggattgttt gttttattac cagtgaattg tttgcaacga
    13981 tatatacatt ggttaaatta cggggattaa ctataggcga gtatcaaagt gaagataata
    14041 atgtggtaaa agattatgtg atgctactga gtacaaatag ccttaataat ttgaatctct
    14101 atttagatag attaatctta ttaccaatta taggtggaac agctgtaact atatcatttc
    14161 tttcaacatt tattgggaaa atgttagcta catttctata tccgattaat aatgtagtac
    14221 tttcatatat ttctgtaaat gaaagtgaca atataaagaa gcaatatttg aaaactaatc
    14281 taattgctat agctgcccta tgtttagtca tgattatatg ttatccaatt acaataatta
    14341 ttgtctcttt actgtataac attgattcaa gtttatattc gaagtttatt attttaggta
    14401 atataggtgt tttattcaat gcagtgagta ttatgatcca aactttaaat acaaaacacg
    14461 catcaataac attacaagcg aattatatga cgcttcacac gattacattt atattcataa
    14521 ctattttaat gacaattgcg tttggtctaa atggattctt ttggacaacg ctgttcagca
    14581 acattattaa gtatgtgatt ttaaatatta taggtttaaa gtctaaattc attaataaaa
    14641 aggacgtcga ttagatgagt gaaaaaaaga ttttgatttt atgtcagtat ttttatccgg
    14701 aatatgtatc ttctgcgacg ttaccaactc aattggcgga agatttaatt gcgaatcaca
    14761 ttaatgtcga tgtcatgtgt ggatggccat atgaatatag taatcataaa caggtttcta
    14821 aaaccgagat gcatcgtggt attcgcattc gacgtctcaa gtattcgagg tttaataaca
    14881 aaagtaaggt tggaaggatc atcaatttct ttagtttatt ttcaaaattc gtgattaata
    14941 tacctaaaat gttgaaatat gatcagattc ttgtttactc taatccacca atcttgccat
    15001 taataccaga cgttttacac agactgctta agaaaaaata ttcttttgtg gtgtatgata
    15061 tagcacctga taatgcgatt aagacaggtg caactcgtcc aggtagcatg attgataagc
    15121 tgatgcgtta cattaataga catgtctaca agaatgctga aaatgtcatt gtccttggta
    15181 cggaaatgaa aaactactta ctaaatcatc aaatttctaa aaatgctgac aatatccatg
    15241 tgattcctaa ctggtatgac atgcgtcaat tacaagacaa tcgtatctat aatgacacat
    15301 ttaaagctta ccgtgagcaa tacgacaaaa ttttattgta tagcggtaat atggggcagt
    15361 tacaggatat ggagacactt atctcatttt taaaattaaa taaggatcag tctcaaacgt
    15421 taacaatact ttgtggtcat ggtaagaaat ttgcagatgt caaaacggca atagaagacc
    15481 atcgtattga aaatgttaaa atgtttgagt ttttaacagg tacagactat gctgacgtat
    15541 taaaaattgc ggatgtatgt attgcatcgc tgattaaaga aggcgtcggt ttaggcgtgc
    15601 cgagcaagaa ttatggctat cttgcagcta agaaagcgtt ggtactcatc atggataagc
    15661 aatctgatat cgttcaacat gttgaacaat atgatgcggg tatccaaatt gataatggcg
    15721 atgcacatgc catttataac ttcatcaaca ctcactcgag taaggaattg cacgagatgg
    15781 gtgagcgcgc acatcaactg tttaaagata aatatacgag agaaattaat actatgaagt
    15841 attacaatct gttgaagtga ggagataatt atgaagcgat tattcgatgt agtgagttca
    15901 atatatggtt tagtagtttt aagtccgatt ctgttaatta cagcattact aattaaaatg
    15961 gaatcacctg gaccagccat tttcaaacaa aaaagaccga cgattaataa tgaattgttt
    16021 aatatttata agtttagatc aatgaaaata gacacaccta atgttgcaac tgatttaatg
    16081 gattcaacat cgtatataac aaagacaggg aaggtcattc gtaagacctc tattgatgaa
    16141 ttgccacaat tattgaatgt tttaaaagga gaaatgtcaa ttgtaggtcc tagaccagcg
    16201 ctttataatc aatacgaatt aatcgaaaaa cgtacaaaag cgaacgtgca tacgattaga
    16261 ccaggtgtga caggactagc tcaagtgatg gggagagatg atatcactga tgatcaaaaa
    16321 gtagcgtatg atcattatta cttaacacat caatctatga tgcttgatat gtatatcata
    16381 tataaaacaa ttaaaaatat cgttacttca gaaggtgtgc atcactaatg agaaaaaata
    16441 ttttaattac aggcgtacat ggatatatcg gtaatgcttt aaaagataag cttattgaac
    16501 aaggacatca agtagatcaa attaatgtta ggaatcaatt atggaagtcg acctcgttca
    16561 aagattatga tgttttaatt catacagcag ctttggttca caacaattca cctcaagcaa
    16621 ggctatctga ttatatgcaa gtgaatatgt tgctgacgaa acaattggca caaaaggcta
    16681 aagctgaaga cgttaaacaa tttattttta tgagtactat ggcagtttat ggaaaagaag
    16741 gtcatgttgg taaatcagat caagttgata cacaaacacc aatgaaccct acgaccaact
    16801 atggtatttc caaaaagttc gctgaacaag cattacaaga attgattagt gattcgttta
    16861 aagtagcaat tgtgagacca ccaatgattt atggtgcaca ttgcccagga aatttccaac
    16921 ggttaatgca attgtcaaag cgattgccaa tcattcccaa tattaacaat cagcgcagtg
    16981 cattatatat taaacatctg acagcattta ttgatcaatt aatatcatta gaagtgacag
    17041 gtgtgtacca tcctcaagat agtttttact ttgatacatc gtcagtaatg tatgaaatac
    17101 gtcgccaatc acatcgtaaa acggtattga tcaacatgcc ttcaatgcta aataagtatt
    17161 ttaataagtt gtcggtcttt agaaaattat tcggcaattt aatatacagc aatacgttat
    17221 atgaaaataa taatgcactt gaaattattc ctggaaaaat gtcacttgtt attgcggaca
    17281 tcatggatga aacgacaacc aaagataagg cataagtcat ctattaaata aaatcaacat
    17341 acaaatcgtt ttatttggag gttatagtat gaagttaaca gtagttggct taggttatat
    17401 tggtttacca acatcaatta tgtttgcaaa acatggcgtc gatgtgcttg gtgttgatat
    17461 taatcagcaa acgattgata agttacaaag tggtcaaatt agtattgaag aacctggatt
    17521 acaagaggtt tatgaagagg tactgtcatc gggaaaattg aaggtatcta caacgccaga
    17581 tgcatctgat gtttttatca ttgccgttcc gacgccgaat aatgatgatc agtaccggtc
    17641 atgtgacatt tcgctagtta tgcgtgcatt agatagtatt ttatcatttt tagaaaaagg
    17701 aaataccatt attgtagagt cgacaattgc gcctaaaacg atggatgatt ttgtaaaacc
    17761 agtcattgaa aatttagggt ttacaatagg tgaagatatt tatttagtgc attgtccaga
    17821 acgtgtactg ccaggaaaaa ttttagaaga attagttcat aacaatcgta tcattggcgg
    17881 tgtgactgaa gcttgtattg aagcgggtaa acgtgtctat cgcacattcg ttcagggaga
    17941 aatgattgaa acagatgcac gtactgctga aatgagtaag ctaatggaaa acacatatag
    18001 agacgtgaac attgctttag ctaatgaatt aacaaaaatt tgcaataact taaatattaa
    18061 tgtattagat gtgattgaaa tggcaaacaa acatccgcgt gttaacatcc atcagcctgg
    18121 tccaggtgta ggcggtcatt gtttagctgt tgatccgtac tttattattg ctaaagaccc
    18181 tgaaaatgca aagttaattc aaactggacg tgaaattaat aattcaatgc cggcctatgt
    18241 tgttgataca acgaagcaaa tcatcaaagt gttgagcggg aataaagtca cagtatttgg
    18301 tttaacttat aaaggtgatg ttgatgatat aagagaatca ccagcatttg atatttatga
    18361 gctattaaat caagaaccag acatagaagt atgtgcttat gatccacatg ttgaattaga
    18421 ttttgtggaa catgatatgt cacatgctgt caaagacgca tcgctagtat tgattttaag
    18481 tgaccactca gaatttaaaa atttatcgga cagtcatttt gataaaatga agcataaagt
    18541 gatttttgat acaaaaaatg ttgtgaaatc atcatttgaa gatgtatcgt attataatta
    18601 tggcaatata tttaatttta tcgacaaata aaatgtgtca aactagggca tacatgatta
    18661 aggaaagata agctgtcatg tgtttgaact tcagagagga taatgttatg aaaaaaatta
    18721 tggttatttt cggtacgaga cccgaagcaa taaaaatggc accattagta aaagaaattg
    18781 atcataatgg gaactttgaa gcgaacattg tgattacagc acaacataga gatatgttag
    18841 atagtgtgtt aagtatattt gatattcaag ctgatcatga tttaaatatt atgcaagatc
    18901 aacaaacatt agcaggcctt acggcgaatg cacttgctaa acttgatagc atcattaatg
    18961 aggaacaacc ggatatgatt ttagtacatg gtgatactac aacgactttt gtaggaagtt
    19021 tggcagcatt ttatcatcaa attccggtcg gacatgtaga agctggactt cgaacacatc
    19081 agaaatactc accatttcct gaagagttaa atcgagtcat ggtaagtaat attgctgaat
    19141 tgaattttgc gccaacagta attgcagcta aaaatttact ttttgaaaac aaagacaaag
    19201 agcgtatctt tattactgga aatacagtta ttgacgcatt gtcaacaaca gttcaaaatg
    19261 attttgtttc aacgattatt aataaacata aaggcaagaa agttgtttta ctaacagcgc
    19321 atcgtcgtga aaatattggg gaaccgatgc atcagatttt taaagcagta agagatttgg
    19381 cagatgaata taaagatgtt gtcttcattt atccaatgca tcgtaatcca aaggtaagag
    19441 cgattgccga aaaatattta tctgggagaa atcggattga attaattgag ccattagatg
    19501 cgattgagtt ccataatttt acaaatcaat cgtacctcgt gctgacagat tctggtggta
    19561 ttcaagagga ggctcctaca tttggaaaac ctgtgttggt attaaggaat catacagagc
    19621 gtcccgaagg cgttgaggcg ggaacatcga gagtaattgg cacagattat gacaatattg
    19681 ttcgaaatgt gaaacaattg attgaggatg atgaagcgta tcaacgtatg agtcaagcga
    19741 ataatccata tggtgatgga caagcatcac gacgtatttg tgaagcaata gaatattatt
    19801 ttggattgcg cacagacaag ccggatgaat tcgtaccttt acgtcacaaa taataaaaaa
    19861 cccctaatca tgaagttggt ttagacaacc agcggtgact aggggttttt aatatattta
    19921 tttttgatag tggtagccaa tatcatattt gaatacttta tttgataata ttggactttg
    19981 ctgtccatcg tcatcacttt ttaaacgtac atttttatga gcttctttaa atacatcgga
    20041 attcaaccaa ttattaaagc tatcttcaga ttcccaaata gttaagattt taacttcgtc
    20101 tgtatcctcg gtatttaatg ttttagtgac aaacatttgt tggaagcctt caatagtttc
    20161 aataccttgt ctattgtaaa aacgttcaat cgtttcttcc gcactgcctt tttgtaattg
    20221 taatctattt tctgccataa acatgggcaa tcactcctct attttatgat ttgatttggg
    20281 taatgttttt acaaatgtaa agagtacagc ggtttgtatg ataaccatta tgattaatcc
    20341 tacacggact gcaagaacat ccaccatata aattgaaaaa cctattacaa tgtataagct
    20401 aattaaaatt ttaattttct gttgtagcgt gtagcctcga tgtaaataaa agttttctac
    20461 atattcttta taaatttttt gattaataag ccaattgtaa aagcgatctg aacttcgagc
    20521 aaaacaaaaa actgctacga gtaaaaaagg ggtcgttggc agtaaaggta atacggcacc
    20581 tgcaatacca agcgctgtaa atattaagcc aatgacgatt aaaataagtc gcattgaaaa
    20641 aactccattc tagtactaat gcgcatgtaa tattgtttta gtaatataac tcatgctaaa
    20701 tataatgtgt atgataagtg caatgactca gtaaaatgaa acgatgttga attatccttg
    20761 tcacattaac gcattttaag cgcgactttc ataacaacca aactatttaa tgagaattat
    20821 tctcaagtat tatagttata ttatgtgttt tatttttgaa aagtgcaata tgttttcgaa
    20881 aataagatta tttttatgtg caaaaacgac gcaaaagttt taaaaatgag acttctgtga
    20941 gctgattatt ttataaaatg taaacgctta ctatataatg tgaatcatat cgtttaaaag
    21001 cattattaaa tatgatgcta agagatttat attatagcca ataaacaaag gagagataat
    21061 atggcagtaa acgttcgaga ttatattgca gagaattatg gtttatttat caatggggaa
    21121 tttgttaaag gtagcagtga cgaaacaatc gaagtgacta atccagcaac tggagaaaca
    21181 ctatcacata ttacaagagc aaaagataaa gatgtcgatc atgcagtcaa agtggcgcaa
    21241 gaggcatttg aatcatggtc attaacttct aaatcagaac gtgcacaaat gttgcgtgat
    21301 attggtgata aattaatggc acaaaaagat aaaattgcaa tgattgaaac attaaataat
    21361 ggtaaaccga ttcgtgagac aacagcaatt gatattccat ttgctgcaag acatttccat
    21421 tatttcgcaa gtgttattga aacagaagaa ggtacagtga atgatatcga taaagacaca
    21481 atgagtatcg tacgacatga gccgattggc gtcgtaggtg ctgttgttgc ttggaacttc
    21541 ccaatgctat tagctgcatg gaagattgcg ccagccattg ctgcaggtaa tacaattgtg
    21601 attcaacctt cgtcttcaac accattaagt ttattggaag ttgctaaaat tttccaagag
    21661 gtattaccta aaggtgttgt caatatacta acgggtaaag gttcagaatc aggtaatgca
    21721 attttcaatc atgatggtgt agataaatta tcatttacgg gctcaactga tgtaggttat
    21781 caagttgccg aagctgcagc aaaacatcta gtacccgcta cattagagct tggtggtaaa
    21841 agcgccaata tcatattaga tgatgctaat ttagaccttg cagttgaagg tattcagtta
    21901 ggtattttat tcaaccaagg tgaagtatgt agtgcaggtt ctcgattatt agttcatgaa
    21961 aaaatttatg atcaattggt gccacgttta caagaggcat tttcaaatat taaagttgga
    22021 aatccacaag atgaagctac acaaatgggt agtcaaactg gtaaggatca attagataaa
    22081 attcaatcat atattgatgc agcaaaagaa tcagatgcac aaattttagc aggcggtcat
    22141 cgcttaactg aaaatggatt agataaaggg ttcttctttg agccgacatt aattgctgtg
    22201 ccagacaatc atcacaaatt agcacaagaa gaaatatttg gaccagtgtt aacagtgatt
    22261 aaagtgaagg acgatcaaga agcaattgat atagctaatg attctgagta tggtttagca
    22321 ggcggtgtat tttctcaaaa tatcacacgt gcattaaata ttgctaaagc tgtacgtaca
    22381 ggacgtattt ggattaacac ttacaaccaa gtaccagaag gcgcaccatt tggtggttat
    22441 aaaaaatcag gtatcggtcg agaaacttat aaaggtgcgt taagtaacta tcaacaagtt
    22501 aaaaatattt atattgatac aagcaatgct ttaaaaggtt tgtactagaa taaatatcgt
    22561 ttctgaagcg tgtttgtagg tcagtctagc ggtaagtctt aacatttaac ggcgttgttt
    22621 agattttaag caaaacaaaa tatataggaa cacgtatcat gatattagga tataatgact
    22681 aaaataatag cagtaggatg gtttttaatt gcaaatcatc ttactgctgt ttttaattat
    22741 gctaatttgc gatgcggcta ttataaggac agagttgttt attaattatg gtgatttaga
    22801 aatatgaagt tcaatatgca aagtcatcgt ttgttttaat atgcggaaca atcattaaag
    22861 ttattgcgat tttttgaact taatgaaact aaacaataaa tttgagatac ttttttgtca
    22921 tttttatgta actaacacaa taatctcgta cattattaaa attttctata tgataggaat
    22981 aaagcaaagc gcgagtgtgc tgtaaaagtt ttccaaggtg atattacata aagctataaa
    23041 gggtaaagat taatgagttg tcatgtaaat gacgatgatg tataaatcat ggttaattac
    23101 ggaagcatta atattaacct gagaagctat aaagaattat ttttaaaagc gacaatatta
    23161 aatacgacgc atttatttag gagtggcaaa cgtatgaatg ggaaaaaggc gaatacgata
    23221 aacagataca aatattttca tcatgtcaat catcaaaaaa ttcaacaaag ttctaaaaag
    23281 acgctgtggg catcactaat catcacattg ttatttacag tgattgaatt tgtcggaggt
    23341 ttagtatcta attcattggc attactgtca gattcatttc atatgcttag tgatgtatta
    23401 gcacttggtt tatctatgtt ggccatttat tttgcaagta aaaagccgac tgcacgatac
    23461 acatttggat atttaagatt tgagatatta gctgcatttt taaatggttt agcattaatt
    23521 gtaatttcaa tctggatttt atatgaagct attgtacgta ttatttatcc gcaaccaatt
    23581 gaaagtggca ttatgtttat gattgctagt attggtttac tcgtcaatat tattttgact
    23641 gttatccttg taaggtcttt aaaacaagaa gacaatatca atattcaaag tgcattatgg
    23701 catttcatgg gagacttatt gaactctatt ggtgtcatcg ttgcagttgt attgatttac
    23761 tttacaggat ggcgcatcat cgacccaatc attagtattg taatttcact catcatttta
    23821 cgtggtggtt ataaaattac gcgtaatgcg tggttaattt taatggaaag tgtgcctcaa
    23881 catttggata ctgatcaaat tatggcagat attaaaaaca tagatggcat attagatgta
    23941 catgaatttc atttgtggag tattacaaca gagcattatt cattaagtgc ccatgttgtg
    24001 ttagataaaa aatatgaggg tgatgattat caagcgattg atcaagtatc atcattgttg
    24061 aaagaaaaat atggcattgc acattcaacg ttgcaaattg aaaacttgca attgaatcca
    24121 ttagatgagc catacttcga caaattaaca taaataaaac attgtagcgc ctaaaacatt
    24181 aatctatgtc ataggcgcac gtttcgtttt atacttatgt tgcatcattt aaatgatttt
    24241 cgtcaatttc tttgatgcta tctacatcta acacgacatc tttaggtttc aaaatatgaa
    24301 tatgtttttc at
//
LOCUS       DANRDI010000025        23600 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.025, whole genome shotgun sequence.
ACCESSION   DANRDI010000025 DANRDI010000000
VERSION     DANRDI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.025"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..546
                     /gene="lpl9"
                     /locus_tag="PZ377_000770"
     CDS             <1..546
                     /gene="lpl9"
                     /locus_tag="PZ377_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HDM6357656.1"
                     /translation="EMKIQLKGENLESRGAVLEINRNTRTAKGHYIVREVVEDSDGMT
                     HNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNFFVQYGNFKELENYKEDEVSYNP
                     EVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLLLKGSGNLKGSSVGYKNIEFTFI
                     ENKEENIYFTDSIYFNPSEDK"
     gene            729..1541
                     /gene="lpl10"
                     /locus_tag="PZ377_000771"
     CDS             729..1541
                     /gene="lpl10"
                     /locus_tag="PZ377_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HDM6357657.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
     gene            1531..2859
                     /locus_tag="PZ377_000772"
     CDS             1531..2859
                     /locus_tag="PZ377_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357658.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            2856..3179
                     /locus_tag="PZ377_000773"
     CDS             2856..3179
                     /locus_tag="PZ377_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357659.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            3198..3512
                     /locus_tag="PZ377_000774"
     CDS             3198..3512
                     /locus_tag="PZ377_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357660.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(3802..4062)
                     /locus_tag="PZ377_000775"
     CDS             complement(3802..4062)
                     /locus_tag="PZ377_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357661.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            4181..5383
                     /locus_tag="PZ377_000776"
     CDS             4181..5383
                     /locus_tag="PZ377_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDM6357662.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(6042..6104)
                     /locus_tag="PZ377_000777"
     CDS             complement(6042..6104)
                     /locus_tag="PZ377_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDM6357663.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(6168..6236)
                     /locus_tag="PZ377_000778"
     CDS             complement(6168..6236)
                     /locus_tag="PZ377_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDM6357664.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(6288..6353)
                     /locus_tag="PZ377_000779"
     CDS             complement(6288..6353)
                     /locus_tag="PZ377_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDM6357665.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(6385..6450)
                     /locus_tag="PZ377_000780"
     CDS             complement(6385..6450)
                     /locus_tag="PZ377_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDM6357666.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            6971..8455
                     /locus_tag="PZ377_000781"
     CDS             6971..8455
                     /locus_tag="PZ377_000781"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDM6357667.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            8468..11173
                     /locus_tag="PZ377_000782"
     CDS             8468..11173
                     /locus_tag="PZ377_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDM6357668.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            11335..11697
                     /locus_tag="PZ377_000783"
     CDS             11335..11697
                     /locus_tag="PZ377_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDM6357669.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(11926..12342)
                     /locus_tag="PZ377_000784"
     CDS             complement(11926..12342)
                     /locus_tag="PZ377_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357670.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGIVYRLYAYVTHILFM"
     gene            12380..13054
                     /locus_tag="PZ377_000785"
     CDS             12380..13054
                     /locus_tag="PZ377_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDM6357671.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            13091..13825
                     /locus_tag="PZ377_000786"
     CDS             13091..13825
                     /locus_tag="PZ377_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDM6357672.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            14003..14116
                     /locus_tag="PZ377_000787"
     CDS             14003..14116
                     /locus_tag="PZ377_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357673.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            14281..15618
                     /locus_tag="PZ377_000788"
     CDS             14281..15618
                     /locus_tag="PZ377_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDM6357674.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            15835..16749
                     /locus_tag="PZ377_000789"
     CDS             15835..16749
                     /locus_tag="PZ377_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDM6357675.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            16733..17875
                     /locus_tag="PZ377_000790"
     CDS             16733..17875
                     /locus_tag="PZ377_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HDM6357676.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            18169..19194
                     /locus_tag="PZ377_000791"
     CDS             18169..19194
                     /locus_tag="PZ377_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDM6357677.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            19198..19857
                     /locus_tag="PZ377_000792"
     CDS             19198..19857
                     /locus_tag="PZ377_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6357678.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            19894..20736
                     /gene="gmpC"
                     /locus_tag="PZ377_000793"
     CDS             19894..20736
                     /gene="gmpC"
                     /locus_tag="PZ377_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDM6357679.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            21068..22072
                     /gene="aaa"
                     /locus_tag="PZ377_000794"
     CDS             21068..22072
                     /gene="aaa"
                     /locus_tag="PZ377_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDM6357680.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(22257..22526)
                     /locus_tag="PZ377_000795"
     CDS             complement(22257..22526)
                     /locus_tag="PZ377_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357681.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            22675..23070
                     /locus_tag="PZ377_000796"
     CDS             22675..23070
                     /locus_tag="PZ377_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDM6357682.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            23060..23545
                     /locus_tag="PZ377_000797"
     CDS             23060..23545
                     /locus_tag="PZ377_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357683.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
BASE COUNT         8153 a         3406 c         4648 g         7393 t
ORIGIN      
        1 gaaatgaaaa tccaattaaa aggagaaaat ctggaatcta gaggagcagt tttagaaatt
       61 aacagaaata ctagaacggc taaagggcat tatattgtta gagaagttgt tgaagatagc
      121 gatggaatga cacacaatca tacaaaaaga tatcctgtga aaatggaaaa taataaaatg
      181 attccattaa aaccaatcga tgacgaaaaa gtaaaaaaag aaatcgaaga atttaacttc
      241 tttgtacaat acgggaattt caaagaattg gaaaactata aagaggacga agtgtcatat
      301 aacccagaag taccaattta ctctgcaaaa tatcaattga aaaacagtga ttacaatgtt
      361 gaacaattaa ggaagcgata taatatcccg acgcaaaaag cgcccaaatt attattgaaa
      421 ggctcaggta atttaaaagg ttcatcagtc ggatataaaa atattgaatt tacctttatt
      481 gaaaacaagg aagaaaatat ttacttcaca gatagtatct actttaatcc aagcgaggat
      541 aaataaatat aattactaat ataaaaaata acgttagaac atttaataat aaagttaaaa
      601 tgaattacat gtattaaatt gaataagtta gttttgtttg ataacataaa agttgaataa
      661 tcactatttt aataagttgt atgaaatatt caaatgttta taaaattaaa acagagagat
      721 gtgaaatgat ggagtatata aaaaaaattg ctttgtacat gagtgtatta cttttaatca
      781 tttttattgg gggatgtgga aatatgaaag atgaacagaa aaaagaggaa caaacgaata
      841 aaacagattc aaaagaagaa caaatcaaaa agagttttgc gaaaacgtta gatatgtatc
      901 caattaagaa tctcgaggat ttatacgaca aagaaggata tcgagatggt gagtttaaaa
      961 aaggcgataa aggtacgtgg actatactta caggtttttc aaaaagtaac aaaccaggag
     1021 tattagatga tgaaggcatg gtgttatatc ttaatagaaa tgccaaaaag gcaacaggtt
     1081 attattttgt aaataaagtt tatgatgata ttagcaaaaa tcataatgag aaaaaatatc
     1141 gtgttgaact aaaaaataat aagattgttc ttttggataa tgtagaagac aaaaaactta
     1201 aacaaaaaat tgaaaatttt aaatttttta gtcagtatgc tgattttaaa gatttaaaaa
     1261 attatcaaga tggaaatata acaactaatg aaaacgtacc gagctatgaa gcacaatata
     1321 aaatgaacaa tagtgataaa aatgtaaaaa aacttagaga aatttatcca attacaacta
     1381 ataactctcc aaatctaaaa ttatatatag atggtgatat aaaaggaagc tcagtaggat
     1441 ataaaaaaat agaatataaa ttttcaaaag ataaaggtca agagacaaca ttaagagatt
     1501 atttgaattt tggaccgtct gaaggtgaga atgttgagta ggaagtataa aatagattta
     1561 aaagcaatta atcaaaatac tgagtctaca agtgctattt cgaaagcgtc atacgaagtt
     1621 gagaatgcta ataataatgg tctatcaaaa agagacgtga ttaatcaatt taatgatttg
     1681 aaaaaaatga aaaaattccc ttctaatctt gaatatgttg atagttacac tgattctctt
     1741 actggagtaa caacttctgc ttttttaaat aaagatacag gcaaagtaac tctcgggatg
     1801 actgggacta atttacaaga cgaagccttt aaaaagttaa aagaaggtga attttcaaga
     1861 caaaatgtta ccaatgcttt ggaaacagtt aaagatggat atgcagatct taaaatatta
     1921 tattctcctg catctgatca aaactataga tatgcgaata cacaagaatt tataaataaa
     1981 ataaaaagta agtatgacat tgattttatt actggacatt cactaggtgg aagagatgcg
     2041 gtagttctag gaatgagtaa tggtattccg aacattgtgg tttataatcc agctcctatt
     2101 tctataacta gtttgaatcc taattcccca gatggaaaac gtttattaga attatataaa
     2161 aattataaag gtaatattac taggtttgtt gcagaaaatg atgcattgac agaaaatctg
     2221 aagaaatata agcattatgt ttttttcggt aatgataaag tctttaaaaa tggtaaaggt
     2281 catgaaatgg aaggctttct gaccgaagaa gaacaaaaag ctataaaaaa agaacttaaa
     2341 aaactacaag gttatgcaga agaaaataat aagtcatttg taaagaattc aaataatgct
     2401 atctctaaat tagctagtat agaattactt agagcaaata tgatgactac aaatggagga
     2461 ggattatctt cttcgcagca aaaagtttta gaaagtttaa cagctttaac aattgcgcag
     2521 tcattcagtc aactgataga cgatgaaatt aatcaaatca aaaaaatgta taatgaaaag
     2581 aaaaagaaat ttggaaaaaa ttgggaagac gctcaaaaag ctggaaaagc tgtaggtgaa
     2641 gatttgagtg taaatggagt tctcaatgct ttagatgaag gtcaagtgaa tgaaagtagt
     2701 atggtaagag aacctgaaca aatgatatct gcaaaagaaa gacaactttc aacgataggg
     2761 tcttctgtat caaattatat tatgagagtt agactcagta ttaatgaaat cgttgataaa
     2821 gatcaagtgc ttgcatcaca aataggtgga ttactatgat gtttaatcaa attaataata
     2881 aaaatgaatt agaagaatca tatgaatctg agaaaaaacg tatagagaat gaactgcaaa
     2941 atttaaatga acttaggcat agaactcgaa aagaaaatga gcgtagttat gatgtttttc
     3001 aatatttgaa gcacgaaatg aattatagtg aagatgccca aaggaaaatg acgagaaata
     3061 tagaagcgta tgagcaagaa atcaatgaga taattagaaa gcaagaatgg aaattagaag
     3121 aatataaaga agacttaaaa aagtcttatg aaaagcagtt agataagcta agtgactgat
     3181 aatttggagg agattaaatg actctaatag aaccagatat gaccttaaga atgccagata
     3241 taagtactac agtagaaaca cttaatctca tatctaaaat ggaagcgcaa aaagaaaata
     3301 ttcgcacagt tattgcacct gaacataagc ataaatacaa agatattgaa aacggattaa
     3361 aaggtgaaga aaaagtatta attgaacaaa tggcgcaaca ttgcgaagct tttaaagcta
     3421 attttaaagg tgcagctcaa ggagattggg ttaaaagtgc catgtctgag atagacagca
     3481 ttaaggatga cctgaaaaaa attaatagct aaactacata tcatactact aaataaagcg
     3541 accaatgttc agtatattca caactgacac taggccgctt ctttttaatt tatattattc
     3601 ccatgtaaag taattcgaac cgaatcgaaa tatcacactc tcaattgtaa aaaaacaact
     3661 taaattactg tcccgcatta aacatgacca cattcgtaac agcaacaata aaaatcctca
     3721 agtactaaaa gctatgatca ctttatcaaa atcaacgcaa gttaacctct cccgaactac
     3781 ccgttactca tcaccttcac cttacaaacc ataaagcgct gcagttaagt attgaatggc
     3841 atagccaatt gaaatgaaaa tagcaatcgt tataatagca ctgccaatgg tatataaagt
     3901 atgtttcgta gataatcctt cagtctttga accaaaaaca ctgagtagaa taaatgcaat
     3961 accagtaaca tataaaacta aaatgacaaa taacatgttt atacacctcg attctctttc
     4021 gtgagatata tctttgtctc cattataaag taaagcgctc aataatttaa ttaagtatac
     4081 tattttttta ttatcgaaga ttgcgaaaga ggaaacgctc ctatataatg taaaacggaa
     4141 ttattcctat ttacaaataa attgaagtga gggtatatat atggctaaaa ttccagttac
     4201 ggtattaagt ggttatttag gctcggggaa gacaacgttg ttaaatcata ttttacaaaa
     4261 tcgagaaggt cgacgtatcg cggtaattgt aaatgatatg agtgaagtaa atatcgataa
     4321 agatcttgtc gcagatggtg ggggactatc gcgtacagat gaaaaattag tcgaactttc
     4381 taatggttgt atctgttgta cacttagaga cgatttatta aaagaagttg agcgtttagt
     4441 gaaaaaaggt ggcatcgatc aaattgttat tgagtcaaca gggatttcag agccagtacc
     4501 tgttgcacaa actttctcat atattgatga tgaacttggc attgatctta cagcgatttg
     4561 ccgtttagat acaatggtta cagttgtgga tgctaaccgc ttcgtacatg acatcaactc
     4621 agaagattta ttgatggatc gtgatcaaag cgttgacgaa acagatgagc gttcgattgc
     4681 tgatttatta attgaccaag ttgaattttg tgatgtattg attattaata aaattgattt
     4741 aattagtgaa gaagaactag cgaagttaga aaaaatgtta agcgcattgc aaccgactgc
     4801 taaaattatt aagacaacaa attctgaagt agatttaaaa gaagtcttga atacgcagcg
     4861 ttttgatttt gaaaaagcga gcgagtcagc aggatggatc aaagaacttg agtctggtgg
     4921 gcatgcatcg catacacctg aaacagaaga atatggtata tcatcgtttg tatataaacg
     4981 tcgtctacct ttccatgcta aaaggttcaa tgattggtta gaaagcatgc caaataatgt
     5041 cgttcgatca aaaggtatcg tatggctagc acaatacaat cacgtagcat gtttattatc
     5101 tcaagcaggg tcatcttgca atattcatcc agttacatat tgggtggcta gtatgtctga
     5161 agcgcaacaa acacaaatat tagcagaacg tcaagatgtc gcagctgaat gggatccaga
     5221 atatggcgat cgtcatacac aatttgtcat tattggtaca gaattagatg aagaaaaatt
     5281 aacaaaagaa ctcgacgcat gcttagtcaa tgcgcaagaa attgatgcag attggcaaca
     5341 atttgaagat ccatatcaat ggcaaattag accagcacga taagttgaat gaagtataat
     5401 catttttgaa ttgtggctta attgtttgca atttatgaca attaataaaa agttaaggtg
     5461 ttcttaggat ctttttgttt tcaatctatc taaagtgtga tagttttgat aaagcagaaa
     5521 tttgcatttt atccataggg ctaggacatg tatgtgtctt agtccttttt atatttacgt
     5581 tgatgaaaaa tggcaaaacc atcgtaccaa ctgttaattg aaggtacaat cattgtttaa
     5641 cagtcataaa aatgagtgaa gtaaatgcag ctatcaatgt actattaaca cttacggtca
     5701 cacgagtgtt tcagtctaaa cgtgcaacgc aattccgcac gtggccaata tatcttgcgc
     5761 tagatcatga aaatcattaa gatttcgcat gccaaatcat gaatgtctca gaactcgagt
     5821 atcaaacata aaacacgcca caaaaatata acgcgtcaga ctacaaagat cacgaatatt
     5881 ccgtatgtcc cactaagaac gtcctataca aaaaacacca agaccattta tttgcaacaa
     5941 actaacaaga ctcacctcac atcaataaat caacacaaag caaagccacc atccctattg
     6001 gtatagtggc ctgagaattt taagtattca attcgcttaa attattttgc gaaaatgtcg
     6061 ataattgctt tgatgatttt aatgatagta cctacaatag ccatcgtttt gtcctcctgt
     6121 atgttgattt aacatagtga tgagttgttg atcgttaatg tttgagatta gttgttacct
     6181 aaaaatttac caagtaaatc tttaaagaat ttgaataatt ttgctacgaa ttccatgtga
     6241 atggccccct tcaaataaga tgttcatatc tatgagattt ttataactta cttaccagtg
     6301 aatttctcaa ttaatccttt aatgaattta atgattcctg caatgatacc catgtgaaag
     6361 acctcctttg tttgttatga aatcttattt accagtgaat tgttcgatta agcttttgat
     6421 aactttaatg atgccagcga tgatacccat taagattacc tcctttgctt atgagttaac
     6481 ttcattgtac atagttatct tgtgcgtaat tgattttttt cctaacttga attgttatga
     6541 tcataactgc gaatttttta ttttaaaagg acgggaaata ctgcctaact gtacaggtgc
     6601 atttacaaaa tctttacaat cattggttta atagaggtta agctcatgaa tttgacatga
     6661 agcagaaatt taattatatt ttatgttata agtttaaaac gaataacacg ttaggtctcg
     6721 tctaggcaaa gcatatttat gaagatgaat tgaacagaca gaagtaacat tttatataca
     6781 tatgtgcatg ggagcacgct taaagagaac actgcaacaa ggtatatcaa cgtaccgtga
     6841 tgcagtgtct agttaaactg aaacctgcaa tagtaaattt tctgccatta tgtacagaat
     6901 ctactattgt aggtttttat ttttatacat aagcaattat gcgagagatt agaaataagg
     6961 aggttatgca gtgttaagtt ttcaattgct attttcactg tttgttattg cgcttatcat
     7021 tgcattgata agtggcttgt tgtttttagc accagttatg ccaatgagat atattaaatt
     7081 acatttatac atactagtca tgccagtatt atttgcagtc attggctttt tcggtattca
     7141 tggtcaacat gtcttaggtc catttaaaat agatcgttta tcttggttat tagctggctt
     7201 tgtaatggcg cttggcttta ttattcaaaa gttttcaatg cgatatttac taggtgatca
     7261 tcattataga cattactttc cattgttcac tgcgattacg tcgtttgcat ctttagcatg
     7321 gatgtctgaa gacttaagac tgatggcact ctgctggggt attacattat tatgtttaac
     7381 attgctgatg aacgttaatc gtttttggaa agtgccacgt gagtctgcga aattatcaag
     7441 catgacattt ttatgtggtt ggcttgcatt cgttggagca attgtaacta tttatattgc
     7501 gactggcgag tggcgggtac cacaacatat agttcatccg acatggtcat tgttgacgaa
     7561 tgtactactt gtattagctg tcatgatacc ggcagcacaa tttccttttc atcgatggtt
     7621 gattgaatct gtaacggcac caacgccagt atcggcaatt atgcatgcag gaattgtgaa
     7681 tgcaggtggt gttattctaa ctcgttttgc gccgatattt gataatggat ttgcgttatc
     7741 attattactt atcctttcta gtatttctgt attgttagga tcgggtatta gcttagttca
     7801 agttgactat aaacgccaat tagtgggctc tacgatgagt caaatgggct ttatgttagt
     7861 tcaatgtgca ttgggtgtat attcagcagc gattattcat ttaatattgc acggtatttt
     7921 taaagcaaca ttatttttac aatcaggttc tatcgtgaag cgattcaata ttccaaaaca
     7981 agcatctgct aaagacgctt atggctggat tgtcatggga cgtgtattag ctattatcgt
     8041 ggcatttcta ttttggatga gtagtgacag aagtgcatat gaagtgttaa gtgcactcat
     8101 tctaggatgg tcattacttg tatcttggaa tcaaatggta gcctttagta aaggacgcat
     8161 ggcacgtctt gttggtatga ttttgattgc aatcgtgaca tttatctacg tcatcacaca
     8221 taattatttt tacgctgtat tacaaaatat aacaacacat gcgacaacgc cgcctacagt
     8281 gagtgtcatc attagtgttg ttattttaat ctttggcagt ttattaagta tttgggtggc
     8341 gcggcatcga tactctaagg cttttgcggt attgtacgtg tggttagtta atctaggtga
     8401 agcacgctcg aaagcgatag aaagtcatcc gaattatttg aagaagtatt tataggaggt
     8461 gaaaggtatg acaacacagt taaatatcaa ttcagtcatt gaaaatgcga aacgtgttat
     8521 tacaccatta tcaccaattt cgatttttgc agcacgaaat ccatgggaag gattagaagc
     8581 ggatacgttt gaagatgtcg caaaatggtt acgtgatgtt cgagatgtgg atattttccc
     8641 aaataaagca ttaatagaaa gcgctgtggc acgtggtgaa ttagatgaaa gtgtctttaa
     8701 tcaacttgtt actgatatgt tacttgaaca tcactacaat atcccgcaac actacatcaa
     8761 tctttatatt gataacatta aaacattaaa agacgtacct gcatcatata tgaatcattc
     8821 aaatgttgat gttgttgctg atctactatt agaaaaatca aaacgtgata tggctgaatc
     8881 atatcatcac tatgatgtac gtccgatgag tgatgcaata atagatgaac aaggtgagcc
     8941 acttagcgaa caagtgaatc gtcaaatgat taaatggacg aaactttata tcgatcaatt
     9001 tctatcgagt tggacaatgc cgaagcgtga gcaaagtttt taccatgcat ggttgcattt
     9061 agcgcaacat gaccatagtt ttactaaagc acagcgccaa gtgattaaag gcttacccaa
     9121 tgatcctgaa atgacgatag agtcagtatt aactcatttt tcaatagatc aggaagacta
     9181 ccaagcttat gttgaaggac atcttttggc gttaccgggt tgggcaggta tgttgtatta
     9241 ccgttcacaa cagcattact ttgaacaaca tttgttaacg gattatttgg caattcggtt
     9301 agttgtcgaa caattgctag ttggtgatga gtttaagtca gtcgctaaag attgtgaaag
     9361 tagatcggaa aattggttta agcaaactgt tgcatcatgg tgttactaca gtgatatgcc
     9421 tagcgatgta ttactacaac atgacgtcaa tgaaattcaa acgtttattc attttgcagc
     9481 aactatgaat aaaaatgtat ttaaaaattt atggctaatt gcctgggaaa tgacatacga
     9541 atctcagtta aaacaaaaaa ttaaagcagg tcatgaaagt gtggcgggcg cattagatgt
     9601 aaaccaagta aatgtctcag aaaatgataa cgctaatcag ccacattcag tattgttaaa
     9661 tgacacacaa gcagttgatg aaaataatag cgagctaaat cagatgggca catcaacgaa
     9721 agcgcaaatt gcattttgta tagatgttcg ttcagaacca tttcgtagac atatcgaagc
     9781 agcagggccc tttgaaacga ttggtattgc aggcttcttt ggattaccta ttcaaaaaga
     9841 tgccgtagac gaacaattca aacatgattc attacctgtc atggtaccgc cggcatatcg
     9901 cattaaagaa tttgcagacc gctacgatat gaatgtttat cgacaacagc aacagacaat
     9961 gtcatcgatg ttttacacat ttaaattgat gaaaaataat gtcatgccta gtctgttatt
    10021 gcctgaatta agtgggccat ttttaagctt aagtaccatt gtcaattcga ttatgcctag
    10081 aaaaagtcgc gcgtctttac aaaaaataaa acaaaagtgg ttgaaaaagc ctgaaacaaa
    10141 gttgacgatt gatcgtgagt ttgaccgaac atcagactta cctgttggat ttactgagca
    10201 agagcaaatt gatttcgcgt tacaagcgtt gaaattgatg gatttaaccg aagcatttgc
    10261 gccgttcgtt gtgttagcag gtcatgctag tcattctcac aataatccac atcatgcatc
    10321 acttgaatgt ggggcttgtg gtggcgcatc aagcggtttt aatgctaagt tattagcgat
    10381 gatatgtaat cgtccaaatg tcagacaagg attaaaacaa tcaggtgtgt atattccaga
    10441 gacaactgtt tttgcggtag cagaacatca tacgtctact gatacgttgg catgggtata
    10501 tgtgccagac acattatctt ctattgcttt agatgcatat gaatcattga atgacgcgat
    10561 gccgatgatt tctgaacacg cgaatcgcga acgtttggac aaactgccaa cgattggtcg
    10621 tgtgaatcat ccagtggaag aagcgcagcg gtttgcgagt gattggagtg aggtacgtcc
    10681 agaatgggga ttggctaaaa atgcatcatt tataattgga cgacgccaat taacaaaagg
    10741 cattgattta gaagggcgga catttttaca caattatgat tggcgtaaag ataaagatgg
    10801 cacattatta aataccatca tttctggtcc ggcacttgtg gcacaatgga ttaatttaca
    10861 atattatgcg tcgacagttg cgccgcattt ttacggaagt gggaataaag cgacacaaac
    10921 cgtcacgtca ggtgttggtg tcatgcaagg taatgcgagt gatctgatgt atggcttatc
    10981 atggcaatct gttatggctg ctgatcggac gatgtatcat tcgccaattc gtttacttgt
    11041 cgttattcag gcacccgact atgttgtagc aagactactc gcgaataatg agcatttcgc
    11101 taggaaggtg tctaatcatt ggctgcgttt aatgagcgtt aatgaggaag ggcgttttaa
    11161 aagttggatt taacgatgat atgttgcgat gctaagtaac taatatatca tcatatctac
    11221 aatgttctag cttcttaaaa tatactaaaa atgataaact taatgtaata gttgatgtcg
    11281 tgataacgat acaaagatga agtaatcaat tttaatatta ttggagttag tgatatgaaa
    11341 agaacgaaag gtgaaatcga agctgaaatc agtaaagcca ttacgcaatg ggaaaaagat
    11401 ttccttggca gaggttcctt gtcagttaaa tcagacattt taagagatat ggtgattatt
    11461 agtttacaag gtatcttaac gccagctgaa tatcgtgtat gtagtacgaa tgaaggatta
    11521 ttaaatatta aacgaacacg ttctgaatta gttgaatccg gtgagcaaga tttgaatgat
    11581 atcattttta aaattacagg tatcaaagtg atgagcttcc atagtgattt aagtacagtt
    11641 acaggtgaac gtattatcgt attcaaactt gaggataatt tggaaaagca tatttaagaa
    11701 ggttaggtat gcttctgctg gcttattaaa gctatagaag tatgcttggt taaacagcgc
    11761 aacgccatat atttgggtgt gctgtttttc tattacatga tgtgtttatt acgtggatga
    11821 atgcgtgggt ctgaatatag cccgtgaaag gcgtatgtca taaaatttgc gaatggtgac
    11881 aagtcgacgc aagcatattt gttgcgagac atgcccacta tccttttaca taaagagtat
    11941 atgtgtgacg taggcatata atcgataaac tattcctaaa aaattaggta taatgaccat
    12001 tgttgcaata agtttttcgg ccttgaaacc gataacgcat agtataatag gaataaaata
    12061 taatacaaat atccagtaaa tcaaatttga aaatgatgga tgaatagggg taaagaatct
    12121 aactaatgta atgattaacg ctatatagac aaaaataact ctattgatac gccttacccc
    12181 ctctgtataa taaatataaa tcgtactaaa ttgaaaatag caaaatattg ttatttcaat
    12241 tatacaatgt tttatttgca atatacataa caatatgaac tttataattt tgatattgtc
    12301 ggtaaagtga aatttgtggc acattgatat tatggataac atttgtaaaa tgaaaatgat
    12361 gatacgagga gattgtaaga tgatagataa aaaattaaca tcaccgaaaa tgacagtgcc
    12421 tttattttta atcgcgctga ttgtatttat aggtatgttt tacagtgtag tgacaaatca
    12481 agaatggctt aaaaatatag atatgggatc attaacatgg tttacagatt atttcggtga
    12541 gccacaacgt cagtatgtta acaatttgtt taattactat atgacgttta gtgcggaaat
    12601 tggagatgtc aaaggtgtcg tgttgatttc cattatcgtc acaatcatac tgtttattaa
    12661 acagaggcat ttagcggttt ggtttgtgac atatttggtt tcaggtgtca tcatgaacaa
    12721 attaattaaa gatactgtat tacgtccaag accatataat catttagccg ttgatacagg
    12781 cttttcattt ccgagtggac attccaacgc cagcacatta ttatatttcg ccttaatgat
    12841 cattattatt tcacttgctg ctaagacaat aacaaaagtg ttgagtgcgt tggttatggg
    12901 aatattatgg cttagcatat tattttgtcg cctttatttt catgcacatt acttttcaga
    12961 tgtcattggc ggcacgtcac tagcaatcat ttgggtagcg ttattcttaa tggtataccc
    13021 atactttatt aatcatcgac gacaacgcgt ttagcaaaca tctttagaca tagtggaggt
    13081 atatagaaaa atgagaatta aaacaccgag tccatcgtat ttaaaaggca caaatggaca
    13141 tgcgatatta ttattacatt catttacagg tacaaatcgg gatgtgaagc atcttgcagc
    13201 tgagttaaat gaccaaggat ttagttgtta tgcaccgaat tatccaggtc atggtttatt
    13261 gttgaaagat ttcatgacat ataatgtaga tgattggtgg gaagaagttg agaaagctta
    13321 ccaattttta gtcaatgaag gttatgaatc tatcagtgca acgggtgtgt ctttaggtgg
    13381 attaatgaca ttaaaattgg cgcaacacta tcctttgaaa cgtatcgctg tcatgtcagc
    13441 accaaaggaa aagagtgacg atggcttaat agaacattta gtttattata gtcaacgcat
    13501 gtcgaatatt ttaaatttag atcagcaagc atcgagtgcg caattagcag caattgatga
    13561 ttatgaaggt gaaattacga agtttcaaca ttttattgat gatatcatga caaatttaaa
    13621 tgttattaaa atgccagcta atatattatt tggtggtaaa gatgcgccat cctatgaaac
    13681 aagtgcacat tttatttatg aacatttagg atcagtagac aaagaattaa atggtctgaa
    13741 ggattcgcat catttaatga cgcatggaga aggcagagat attttagaag aaaatgttat
    13801 tcgctttttc aatgctttaa cataattgaa gattttaaaa attaacactt gagtgacgat
    13861 gttattatat ttgtcatttg agtgtttttt gtggcgtgat agtataggga acttagctaa
    13921 agttaatttg tagtgtgatg tcgtggaaat attatggagt tataccagaa gttgatttgt
    13981 attgtaatgt cgtggaaatt ttatgggcat tgcacctgaa gttgttatgt ggcgagatgt
    14041 tggggatata gatggtgttg tatgtggcga gatattggcg cacgtataca gcgttgaacc
    14101 agaaaattta atgtagcgta atgacaacac attacaagag ttgattatat tatgataaat
    14161 ccaggtgcta ccgtattaat gaaacaattt tgatgcaggt gtattgcatg ttatttacat
    14221 acatgttaga gtgttactta aacacgtaga cgctttaacg ctttaaagga gatgtgaatg
    14281 atgaaaagac aacaatcaca atggaagtca tcaactggat ttattttagc tagtgcgggt
    14341 tctgcaatcg gtcttggtgc catgtggaaa ttcccatata tggcagggat ttatggcggc
    14401 ggtgcctttc tagctatgtt cttaatattc accatttttg ttgggttgcc attactcatt
    14461 atggaattca ctgttgggaa aatgggacgg acatatacaa cacaaatata tagtaaatta
    14521 actggtaaaa aatggctcaa tatcattggc tggaacggta atttggcagt gtttatttta
    14581 tttggcttct atagtgttat cggtggttgg attgtcattt acatcggaca agtattatgg
    14641 caattagtta tatttcaacg catcaatcat ctccaagaaa tgaattttga agcggtaata
    14701 tcaaatcctt ggttaaccgt tctagggcaa ggtatattca tattcgctac gatgattatt
    14761 gtcatgttag gtgttgaaaa aggattagaa aaggcatcga aagttatgat gccattgctg
    14821 tttgtctttt taatcgtcat tgtgattaag tctttaacat tagatggcgc cttagaaggt
    14881 gtgaaattta ttttacaacc aagagtatca gagattactg ctgatggcat cttgtttgcg
    14941 ctaggtcaat cattctttac gttatcatta ggaactacag gtatgattac ttatgcgagt
    15001 tatgcctcta aagacatgac gattaagtca tcagctattt ctatcgttgt tatgaatatc
    15061 tttgtatctg tattggcagg tctagctata tttccggctt tacatagttt tggctatgaa
    15121 ccacaagaag ggcctggatt attatttaaa gtactgccaa tggtctttag tcaaatgcat
    15181 ctaggcacat tattctattt gggattctta gtgctgttct tatttgcggc tttaacgtca
    15241 tctatttctt tattagaatt aaatgtttct aacttcacga agaatgacaa tacaaaacgt
    15301 aaaaaagtcg cagtgatcgg tagtatttta gtatttatca ttagtattcc agcaacctta
    15361 tcttttggta tcttaaaaga tgtaagattc ggtgcgggaa cgatttttga taatatggat
    15421 ttcatcgttt cgaatgtatt gatgccatta ggcgcattag gtactacgct tgtcgtagga
    15481 caattattag ataaaaaatt attacaacaa tattttggta aagatcgatt tagattattc
    15541 agtggttggt attacttaat taagtatgcg atgcctgtcg ttattatttt agtctttatc
    15601 gtgcaattat ttagttaata tatagaagca tctgacacac aagtatttgt gttgctcagg
    15661 tgtttttttg tttggaattg aaaatttagt actggttctg aattaatcga aacttagata
    15721 attgcaaaat aacaaaatgt cgtttataat tcttatctga aaagtaagaa ttaaaattga
    15781 gacatgaaga aaggtttcat tcatttgatc ggcatgtcag atgagggtgc atctatgatt
    15841 acttatgatt taattggcaa tacaccatta gtactgttag aacattatag tgatgataaa
    15901 gttaaaattt atgccaagct tgaacaatgg aatcctggag gcagtgttaa agacagactc
    15961 gggaaatatt tagtagagaa ggcaattcaa gaagggcgtg tgcgtgcagg tcaaactatt
    16021 gttgaagcga ctgctggtaa tacaggcata gggttagcta ttgcagcgaa tagacatcat
    16081 ttgaaatgta agatctttgc gccgtatggt ttttcagaag aaaagattaa tattatgata
    16141 gcgcttggtg cagaagtttc aaggacgagt cagtctgaag gtatgcatgg ggcacaatta
    16201 gctgcacgtt cctatgctga aaaatatggt gccgtttata tgaatcaatt tgaatccgaa
    16261 cataatccgg atacatattt tcatacattg ggacccgaat tgacttcagc attacagcaa
    16321 attgattatt ttgtggctgg tattggctct ggcggtacat ttacaggtac cgcacgttat
    16381 ttaaagcaac atcacgtgca atgttatgcc gttgagccag aagggtccgt gttaaatgga
    16441 gggccagctc atgcacatga cactgaaggt atcggttctg agaaatggcc gatattttta
    16501 gagagacgtc ttgtagatgg gatatttacg attaaagatc aagatgcctt tcgaaatgtc
    16561 aaaagtttgg ctataaatga agggttgtta gtaggcagtt cttcaggtgc agcattacaa
    16621 ggtgcattga atttaaaagc gcaattatct gaaggtacga ttgttgtcgt atttccagat
    16681 ggtagtgatc gatatatgtc taagcaaata tttaattatg aggagaataa taatgaacaa
    16741 gaaaactaaa ttaattcatg gtgggcacac aacagacgat tatacaggtg ccgttacaac
    16801 accaatttat caaacaagta catatttaca agatgatatt ggtgatttac gtcaaggata
    16861 tgaatattct cgtactgcga atccaacaag aagttctgta gaaagcgtta ttgcgacatt
    16921 agaaaatggc aaacatggct ttgcatttag ttcaggtgtt gcagcaatca gtgcagttgt
    16981 tatgctgttg gacaaaggag atcatattat tttaaattca gatgtatacg gcggtactta
    17041 tcgcgcattg acaaaagtat ttacacgatt tggcattgaa gtggattttg tagatacaac
    17101 gcatacagat tcaattgtac aagcgatacg cccaacaaca aagatgttgt ttattgaaac
    17161 accttctaat ccattattac gtgttactga cattaaaaag tctgctgaaa ttgcgaaaga
    17221 acacggtttg atttcagttg ttgataacac atttatgaca ccttattatc agaatccatt
    17281 agatttaggt atcgatattg tcttacattc tgcaacgaaa tatttaggtg gacatagtga
    17341 tgtcgttgct ggtttagttg caacatcgga tgacaagctt gcagaacgtt tagcatttat
    17401 ttcaaattca acaggtggca ttttaggacc tcaagatagc tatttacttg tgaggggtat
    17461 taaaacatta ggtttacgta tggaacaaat taatcgcagc gttattgaaa ttattaaaat
    17521 gttacaagca catccagctg tgcaacaagt gttccatcca agtattgaaa gtcatttaaa
    17581 tcatgatgtc catatggctc aagcggatgg ccatacaggt gtgattgcat ttgaagtgaa
    17641 aaatacagaa agtgccaaac aattgattaa agcaacatcg tattacacat tagctgaaag
    17701 tttaggtgca gtggaaagtt taatttcagt acctgcattg atgacacatg catccattcc
    17761 agcagatatt cgagctaaag aaggtattac agacggactt gtaagaattt ctgtaggtat
    17821 tgaagatact gaagatttag tcgatgattt aaaacaagca ctagatactt tataaataat
    17881 agcagcactg gcatatattt tgagttcaac gtttgttgtt cttgaatatg ctagtgcttt
    17941 tttgtgcgat gttaatattt agtaattgcg ttgtggagga ttggtggaca tttttatgga
    18001 tttaatttga taaatgtcat agtagtctca caattcgtca ttgtcacatg aattacttat
    18061 tttttaattt tttagaaaat tcggcaaatt gtattgacga tatatgcaat tttacatata
    18121 atgctcttat tcctagtgga ttaataagaa tttgtaggag gggcgatgat gattgagttt
    18181 cgacaggtaa gtaagacctt taataaaaag aagcaaaaaa tagatgcttt gaaggacgta
    18241 tcatttacgg tcaatcgcaa tgatattttt ggtgtgattg gatatagtgg tgcaggaaaa
    18301 agtacgttgg taagactcgt gaatcatctt gaagctgcct cgaatggaca agtgattgta
    18361 gatggacatg atattacgaa ttatagcgat aaaatgatga gggatattaa gaaagatatc
    18421 ggtatgatat ttcagcattt caatttatta aattcagcta ccgtatttaa aaatgtagca
    18481 atgccactca ttttaagtaa gaaaagcaaa acagaaatta agcaacgagt aacggaaatg
    18541 cttgaatttg taggattgag tgataaaaaa gaccaatttc ctgatgaatt atctggtggg
    18601 cagaagcaaa gggtggctat tgcaagagcg cttgttacta atccgaaaat actcctatgc
    18661 gatgaagcaa caagcgcatt ggatccagca acgactgctt cgatattgac gttattaaag
    18721 aatgtcaatc aaacctttgg cattacaatt atgatgatta cacatgaaat gcgcgttatt
    18781 aaagacattt gtaatcgtgt tgctgtaatg gaaaagggga aagtggttga aacaggaact
    18841 gttaaagagg tgtttagtca tcctaaaacg acgattgctc aaaattttgt gtctacagtt
    18901 atacagactg agccaagtac atcattgatt cgtcgattga atgacgaaca agttggcgat
    18961 tttaaagatt ataaaatctt cgtcgaggaa actcaggtga cacaaccgat tataaatgac
    19021 ttgattcaaa tttgtggcag agaggttaaa attttatttt catctatgtc agaaatacaa
    19081 ggtaacaccg tatgttatat gtggcttcga tttaatatgg atcaacaatt tgaagacacg
    19141 gcaataaatc aatatttcaa agagaaaaat attcaatttg aggaggtgca ttaacatatg
    19201 tttggttctg atttagacag tgcacagtta ttacaagcat tgtacgaaac gctatatatg
    19261 gtatctattg ctttattttt aggagcagtg attggtattc cattaggtgt cttattggta
    19321 attactcgaa aacaaggcat atggcccaat atagtgatac atcaagtttt aaatccttta
    19381 atcaatattt taaggtcact accatttatt attttgttaa ttgcgattgt gccattcaca
    19441 aaattagtag taggtacttc aattggtacg actgctgcca tcgtgccttt aacagtatat
    19501 gtggcacctt acattgcaag acttgttgaa aactcattat tggaagtaga cgaggggatt
    19561 attgaagcgg cgaaagcgat gggcgcttca ccactacaaa tcattagata ttttttaatt
    19621 cctgaagctt taggttcgtt agtattagca attaccactg cgattattgg acttattgga
    19681 agtacggcga tggcaggagc tgttggcggt ggtggtatag gagacttagc tttagtgtat
    19741 ggttatcaaa gatttgatac gacggtcatt attattaccg ttattgtatt agtcattatt
    19801 gtccaagtga ttcaaacgct agggaatgtt ctagctagat tcatacgtag acattaatga
    19861 tatatagtga agattttgaa aggaattgat agaatgaaaa gattgattgg gttagttatc
    19921 gtagcacttg tattattagc agcgtgtggt ggtaacaatg ataaaaaagt aacaattggt
    19981 gtcgcatcaa atgacactaa ggcttgggag aaggttaaag aattagctaa aaaagatgat
    20041 attgatgtgg agattaagca cttctcagat tacaatttac cgaataaagc attaaatgat
    20101 ggtgatattg atatgaatgc attccaacat tttgcatttt tagatcaata taaaaaggcg
    20161 cataaaggaa caaagatttc agcattaagt acaacagttt tagcaccgtt gggcatttac
    20221 tcagataaaa ttaaagatgt caaaaaggtt aaagatggtg ctaaagttgt cattccaaat
    20281 gatgtgtcaa accaagcacg tgcacttaaa ctattagaag cagctggttt aataaaactg
    20341 aaaaaagatt tcggattagc aggcacggtg aaagatataa cgtcaaatcc aaaacattta
    20401 aaaattactg cagtagatgc acaacaaact gcacgtgctt tatctgatgt cgatattgca
    20461 gttattaata acggtgtagc aactaaagcg ggtaaagatc ctaaaaatga tccgatattt
    20521 ttagaaaaat caaattcaga tgcagtaaag ccatatatta atattgttgc agttaatgac
    20581 aaagacttgg ataacaaaac atatgctaaa atcgtagaat tgtatcattc aaaagaagct
    20641 caaaaagcgt tgcaggaaga tgtcaaagat ggagagaaac ctgttaattt atctaaagat
    20701 gagattaagg caatagaaac gtcattagca aaataaatta tattgcgtcc tacaagcaaa
    20761 gttcatgctt atgtttgtag ggcgttattg ttggagaata aaattatttc caatagagaa
    20821 agggattgta atcattttat agtgaaatat tatgaaattg taataattta gatattgtaa
    20881 aatctaataa gttgtaataa ttttaagggg taattataaa atttgatgat acagtatatg
    20941 atttttttgt aatcataatg tcatcaaaca tcaacctatt atacataata aaatcgtata
    21001 atgatgtagt attcataaat tcggataaaa gaatgttagg aaagttaagc aagaggagga
    21061 ttttaaagtg caaaaaaaag taattgcagc tattattggg acaagcgcga ttagcgctgt
    21121 tgcggcaact caagcaaatg cggctacaac tcacacagta aaaccgggtg aatcagtgtg
    21181 ggcaatttca aataagtatg ggatttcgat tgctaaatta aagtcattaa acaatttaac
    21241 atctaatcta attttcccaa accaagtact aaaagtatct ggctcaagta attctacgag
    21301 taatagtagc cgtccatcaa cgaactcagg tggcggatca tactacacag tacaagcagg
    21361 cgactcatta tcattaatcg catcaaaata tggtacaact taccaaaaca ttatgcgact
    21421 taatggttta aataatttct ttatttatcc aggtcaaaaa ttaaaagtat caggtactgc
    21481 tagctcaagt aacgctgcga gcaatagtag ccgtccatca acgaactcag gtggcggatc
    21541 atactataca gtacaagcag gtgactcatt gtcattaatt gcatcaaaat atggtacaac
    21601 ttatcaaaaa attatgagct taaatggctt aaataatttc tttatatatc cgggtcaaaa
    21661 attgaaagta actggtaatg catctacgaa ctcaggatct gcaacaacga caaatagagg
    21721 ttacaataca ccagtattca gtcaccaaaa cttatataca tggggtcaat gtacatatca
    21781 tgtatttaat cgtcgtgctg aaattggtaa aggtattagt acttattggt ggaatgctaa
    21841 taactgggat aacgcagcgg cagcagatgg ttacactatc gacaatagac ctactgtagg
    21901 ttctatcgct caaacagatg taggttacta tggtcatgtt atgtttgtag aacgtgtaaa
    21961 taacgatggt agtattttag tttcagaaat gaactattca gctgcaccag gtattttaac
    22021 ttacagaacg gtaccagctt accaagtaaa taattataga tatattcact aaagtcttac
    22081 gtatataaat atataatgaa ttcctattac atttcacaag ctgaaaagtt ggattgaatc
    22141 ctaaatttta tggcggggct atcaaagtcg tgattgttgt aataggaatt tttgtatatg
    22201 aaaaaggatt ggtcgaatga acttcatgtt ctatgttcga ccaatccttt tctgaattag
    22261 tattcatgtt ttactttgcg aatgcttgta aataatctag caccgtttgt tattaaagta
    22321 acaactgcca ctgctttttt gaacttacgt ggtgacttaa ttgaaatgta aaagtctaac
    22381 acggttccta cagtgcttaa acctaatgaa agatatacta attttttatt tttagcatga
    22441 tatttatagc cattgtagcc gtcgactatg aaacctgcga catttagtaa acttgataaa
    22501 cgttgtgatt tggaacgttt tgccataata ataattcccc ctagtatctt ctgtttatat
    22561 gaaaagttgg gtgcgctgaa ttgctaacgt tttgcgctat aactactcat atatgataac
    22621 ataattgtac agtataattt gaaaaattga tttcacaaag ttggggtgtc aaagatgatt
    22681 aaatgtgtct gtttagttga agaaacagct gataaaatat tgcttgttca agtaaggaat
    22741 cgcgaaaagt attatttccc gggtggtaaa atagaagaag gggaatcaca agtacacgcg
    22801 ctgttaagag aagtaaaaga agaattaaat ttaacattaa caatggatga aattgaatat
    22861 gtcgggacaa ttgtaggtcc tgcatatcca caacaggata tgttaactga gttaaatgga
    22921 tttcgcgcat taaccaaaat cgattgggaa aacgtaacta tcaataatga aattacggat
    22981 atacgctgga ttgataaaga taatgatgcg ttgattgcgc ctgctgtcaa agtttggatt
    23041 gaaacttatg gtggtaaaca tgacaaataa tgacaccatc atgttacgac attatgtccc
    23101 acaagattat tcgatgttag aagcttttca attaagtgaa agtgatttga agtttgttaa
    23161 aacgccagag gaaaatatta cagctgcaat gtctgataat gaaaggtatc ccatcgttgt
    23221 aatggatggc aggcaatgtg tggccttttt tacattacat cgtggaaaag gggtcgcacc
    23281 atttagcgat aaccaagatg cagtattttt caggtcattt agtgttgatc aacgttatcg
    23341 taatagagga ataggtaaag tggtaatgga aaaattggcg tcatttatca cttcaacatt
    23401 tcaggatatt aatgagattg tgttaacggt taatactgac aatccacatg ccatggcact
    23461 ttatcgccaa caaggatatc aatatatggg agatagtatg ttcgtcggaa gacctgttca
    23521 tattatggcg ttaactataa aataaattaa atttaaaagc atctttactc atcgtcgacc
    23581 acaacaatta atgatgaata
//
LOCUS       DANRDI010000026        23461 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.026, whole genome shotgun sequence.
ACCESSION   DANRDI010000026 DANRDI010000000
VERSION     DANRDI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.026"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..536
                     /locus_tag="PZ377_000798"
     CDS             <1..536
                     /locus_tag="PZ377_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357684.1"
                     /translation="LEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTNRFSMVMNDLL
                     QLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMPVKSLLECVKR
                     KEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMNHISEKWPTIT
                     NNWLYHWAQYIKTIFNK"
     gene            549..1193
                     /locus_tag="PZ377_000799"
     CDS             549..1193
                     /locus_tag="PZ377_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDM6357685.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(1521..2000)
                     /locus_tag="PZ377_000800"
     CDS             complement(1521..2000)
                     /locus_tag="PZ377_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDM6357686.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(2270..3034)
                     /gene="argB"
                     /locus_tag="PZ377_000801"
     CDS             complement(2270..3034)
                     /gene="argB"
                     /locus_tag="PZ377_000801"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDM6357687.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            complement(3050..4291)
                     /gene="argJ"
                     /locus_tag="PZ377_000802"
     CDS             complement(3050..4291)
                     /gene="argJ"
                     /locus_tag="PZ377_000802"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDM6357688.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            complement(4303..5334)
                     /locus_tag="PZ377_000803"
     CDS             complement(4303..5334)
                     /locus_tag="PZ377_000803"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDM6357689.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            complement(5373..6557)
                     /gene="rocD"
                     /locus_tag="PZ377_000804"
     CDS             complement(5373..6557)
                     /gene="rocD"
                     /locus_tag="PZ377_000804"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDM6357690.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            complement(6810..8165)
                     /gene="brnQ"
                     /locus_tag="PZ377_000805"
     CDS             complement(6810..8165)
                     /gene="brnQ"
                     /locus_tag="PZ377_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDM6357691.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            complement(8441..8998)
                     /locus_tag="PZ377_000806"
     CDS             complement(8441..8998)
                     /locus_tag="PZ377_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDM6357692.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(9065..10705)
                     /locus_tag="PZ377_000807"
     CDS             complement(9065..10705)
                     /locus_tag="PZ377_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDM6357693.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            10899..11000
                     /locus_tag="PZ377_000808"
     CDS             10899..11000
                     /locus_tag="PZ377_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357694.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(10979..13024)
                     /locus_tag="PZ377_000809"
     CDS             complement(10979..13024)
                     /locus_tag="PZ377_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDM6357695.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            13610..14665
                     /locus_tag="PZ377_000810"
     CDS             13610..14665
                     /locus_tag="PZ377_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDM6357696.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            14665..15561
                     /gene="murQ"
                     /locus_tag="PZ377_000811"
     CDS             14665..15561
                     /gene="murQ"
                     /locus_tag="PZ377_000811"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDM6357697.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            15573..17027
                     /locus_tag="PZ377_000812"
     CDS             15573..17027
                     /locus_tag="PZ377_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDM6357698.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            17030..17905
                     /locus_tag="PZ377_000813"
     CDS             17030..17905
                     /locus_tag="PZ377_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDM6357699.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(18099..18380)
                     /locus_tag="PZ377_000814"
     CDS             complement(18099..18380)
                     /locus_tag="PZ377_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357700.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            18593..21382
                     /locus_tag="PZ377_000815"
     CDS             18593..21382
                     /locus_tag="PZ377_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDM6357701.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            21587..22429
                     /locus_tag="PZ377_000816"
     CDS             21587..22429
                     /locus_tag="PZ377_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HDM6357702.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            22431..23354
                     /locus_tag="PZ377_000817"
     CDS             22431..23354
                     /locus_tag="PZ377_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDM6357703.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            23341..>23461
                     /locus_tag="PZ377_000818"
     CDS             23341..>23461
                     /locus_tag="PZ377_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HDM6357704.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFD"
BASE COUNT         7975 a         3674 c         4391 g         7421 t
ORIGIN      
        1 tattagaagc tgtaaataat ggcttagatg gtcggattgt acgtgttggt aatttgacga
       61 atccttacaa tggaagatgg catatgagaa atataaagac taaccgtttt tcaatggtaa
      121 tgaatgattt gttacaactg gattgtatcg gggttagcat ggctgaaatg cctgtagatt
      181 tttcttttgt ggatacgact gcaagacaaa ttgtcgcatt agcacaggtc aacacaccac
      241 aaatcattta ccatgtgcta tcacctaata aaatgccggt gaaatctttg ttagaatgcg
      301 ttaagcgcaa agaaattgaa ctcgtcagcg atgaatcatt taatgaaatt ttacagaaac
      361 aagacatgta cgaaacgatt ggattaacta gtgttgaccg tgaacaacaa ctagcaatga
      421 tagatacaac attaacatta aaaataatga atcacatcag tgaaaaatgg ccaacgataa
      481 ctaacaattg gctgtatcat tgggcacaat atatcaaaac aatattcaat aagtaagtag
      541 ggaaagttat gacagtattt gtaatgcaat tacagagtaa cttgaaaagt attgaagaat
      601 taatatcaca aagtcgttgg tcatataaaa aaccgcgtac agtcaactat agatacaatc
      661 aagataaact catgcacaga ttgggagata ttttagtgca atatggaatt caacatgaca
      721 caggtttatt accacatgaa tggcattatc acatttcgcc acgaggtaag gcagatattg
      781 ttcaacacaa tcgtgatgga cagcccatct atgtgagctt atcatatagt tatccttata
      841 tcgtgtgtgt tgtcgataaa gaaccagttg gtattgatat cgaaaagata tcacaacgtt
      901 tagactggcg tacgttagtg acgtgtttct ctacaaacga agcacatcaa atatgtagtt
      961 taaatgattt ttatcaaata tggacacaaa aagaaagttt tacaaaattg attggtgaag
     1021 gtttaatcaa aggattggac atttatgata tgacacaatc acacttttat caatcacgtg
     1081 aagtgaagtt caaacaattt atttttgatc agtttatggt acaggtatgt ttcttaggac
     1141 aggcaccctg gggttataaa aaagtgtctg tatttcagtt attgagtagt taatatgacg
     1201 tctgacagta tcattgcgca gtgattttaa actatactgc ctaaatgttt actgttagat
     1261 gtttttttat tggaaaaatg aaaggtgaat acacgatggc atcattcaaa atcatgacga
     1321 gattaaagta agtgataagg taaaaatata ctgagcaata ttgaaattac cgaaaataat
     1381 agtttgttac gacgcatttg agactttaat gctcacgtaa gttggctgga atcatatcat
     1441 gtcagttaat aaaataacgt gagcatcaat catcatactc aaacgatgat ttaaaactca
     1501 cgttaatgtt agtcagtgta ttaatttaat ttatagcgat agacaaaatg ttgtcgcact
     1561 aattcaattg tcatcatcca gacgatatgg ccaaagaatt ctgacagatg ctcttggaat
     1621 ggttgatccc acacagcagg tacagtatgc atgattggca taatgataag gtggaataat
     1681 acccaaatag caataccaaa aacagcacct tgtcccattg ctaagtaagc gtatttttta
     1741 actaatatgc agtaaataat tgcaatgacg atagaaaaac taaagtggac aataaagctt
     1801 acccaaggca attccatatt tgaaaatgta tatgtttgat gcgtaaactc actactaaat
     1861 cctaattgtt gcaataactc ttgaggtggg ttcgttgcat tacgttctgg tgtgcgaggt
     1921 ggaaacatga cctcccaacc taattttaca attccagata acaagccacc gataattcca
     1981 gcataaatga atatacccat ttgtcgtttc gtcaacttaa ggactcctct ctatttacta
     2041 tcctcattat agtattttgt gaaaataatc acaataaaaa gctttgcaaa acttaaaata
     2101 ttttgataaa caaatttgta aaaggttatt agttttttac atctcatatt gtatcgtact
     2161 gtattcaaaa ttgttaacga gatgaacggt aaataaatgt tttagcgcaa tggataatcg
     2221 aactggaatc atctcgcgtg tcaaaacttg ttaggcctta atttcatagt tatgaattaa
     2281 ggattgttgt gccaacaaaa tcattattgt aaatagattc aatgatattt ggcttgtttc
     2341 ctgatgcaat gataacttta ggacagccat tttcaatcgc atttttggca tctagcactt
     2401 tgggaatcat acctccataa atatcaccat gttcaatata ttgatgaata tcgactaatg
     2461 gcaattgagg tataacaaca tcattgatga gtacacctgc aatattactt aatacataaa
     2521 taggcgcttt taatgatgat gcaataaaat aggcaagcgt gtcagcatta atattgtaaa
     2581 attctccatc atggttattg aaaccaatcg aattgatgat aggtacaaat ttagtacata
     2641 aatactgtaa agcatcctta tttaaagcgg tcggaacacc gacatatcca tattgttgat
     2701 caaaagatgt aatttcaaac agctgtgcat ccaaaccaca taagcctatt gcagaacatt
     2761 ggtgctggtt aaattgagct actaatgcag tgttaacgtc tgcaatgagc gtgtgtttag
     2821 taatggtcat ggttgcttta tcagtcactc ttaggccatt aacaaagtgt ggctcgattt
     2881 gctggtttga taatgcttca ttaataaatg ggccaccgcc atgaacgata atggggtaga
     2941 tgttgtttga tcgtaaatgc ttaatgttgt taataattga tggatgcatg tcactaagtg
     3001 tactgccacc aattttaatg acaataaatt tcatctaacc aacaccacct tatgttcgat
     3061 atgatgcgtt gatacgcaca taatcatagg ataaatcaca accgtatgca gtcgctgcag
     3121 cgttacctaa accaagctga acgtcaattg tgacattttc atgagttaat gtattcgaca
     3181 tagcttgctc atcaaatagt acagccatac ctttatcaac gacaggtatt tggttcagtt
     3241 gaacatatgt gcagttagga tcaatttcac atccgctgta gccaatagct gtaatgattc
     3301 gaccaaaatt ggcatcttcg ccaaaaatag ctgattttac tagatttgaa cttacgatag
     3361 ttttaccgat ttttcttgca tctgatattg atttagcgcc tgacacattg acgctgatta
     3421 actttgttgc gccttcgcca tctctggcta tagctttagc taaaaatgta cagacaaaat
     3481 tgaatgcatc aacaaatgtt tcccattgtg gatggtcttg actaagtatt tggtgttcaa
     3541 cttggtgatt tgccatgact aataccatgt catttgtact tgtatcgcca tcaacagtaa
     3601 tcatattaaa tgtatggtca gtcgaagatt ttaataattg atgaagtgta ttcgattcaa
     3661 tcgatgcatc ggttgttata aaagcaagca tggtagccat atttgggtga atcatacctg
     3721 aacctttggt gctaccacca attgtaacgg ttttaccatc gatttttagt gatacagcga
     3781 tatgttttgt acaggtatca gttgttaaaa ttgcctcgtt aaacgcacct ggcgttgcaa
     3841 aattagcatc cttaatatgt tcggtcccag tcttaatttt atccataggc aaatattcac
     3901 caatgacccc agttgaagca acagcaacat gctcagatgg tatttgaagt tgttgagcaa
     3961 cccatgtttg tgtttgtcgt gcatcatcta tgccttgttg accggtacaa gaatttgcat
     4021 tagctgaatt aacaacaagt gcttgtaatt ttcctttaga cttttgtaaa gtgtcttcag
     4081 tgacaataag tggtgcagct ttaaactgat ttaaagtata tacggcagct gcacttgcca
     4141 aagacgatga gtaaatccat ccaaagtctt ttttgttagc gcgtaaaccg atgtgcatac
     4201 caccagccgt gaagccttga ggtgtactga tatcgccatg tttaataatt gaaaagttat
     4261 attgttgtga tgtcgtttct tgatgtttca ttctaacacc ccttatggat aaactggtga
     4321 ttgatttagg ccagtcgtca cttcaaaatc atataatata tttaaatttt gaatggcttg
     4381 cccacttgcg cctttgacaa ggttatcaat cactgatact aaaattgctg tttgcgttgt
     4441 ttcatctaca tagatgccga tatcgcagta gttactaccg agtacttctt ttgtggttgg
     4501 aaaagtccca atatctctaa ttctgacaaa tggctgatta gcataataag aggtcattaa
     4561 tttatgtaat gattcagtcg tatattcaga tgataatttg acatatattg ttgataaaat
     4621 acctcgtgtc attggtacga gatgtggtgt aaatatgact gatacatctt gacccgcaat
     4681 gatagataaa tattgctcga tttccggttt gtgtttatgg tttccgattg cataagcgct
     4741 tagattttca ttcatttctg aaaaatgaac acgttgtgat aatgaacgac cagcacctga
     4801 cacgccggtc ttagcatcaa taataataga tgacaaatct actatttttt cgctaataag
     4861 tggatgtaat gctaataatg ttgctgtagg gaaacagcca ggattagaaa tgagcttcgt
     4921 tccattgtta tcaaacgatt gccattctga aatgctgtaa atagcatgat tcaaatcatc
     4981 ttgtgctgca gcagtttctt tgtaatatgc ttcatatatt tcacgattct taattctaaa
     5041 tgcgccagat aaatcgataa catgaatacc tttttctact aagggaggga tacatgtttt
     5101 acttacgggt gctggtgtcg caaagaaaat tacatcacag tcattattgt ccactgtaag
     5161 tgcttcgaaa tgttgcataa tatgttgtaa atgtggaaat gttaatttca acggttcatc
     5221 tacttttgaa tgtgagtaga tgtgtgcaat cgttacatga ggatgtgttt gtaacaatcg
     5281 aattaattca attgcgccat aaccgctacc gccaacgata cctactttaa tcatcatgaa
     5341 tccctcctat gtcatatata aatgattaat tgttaatttt taaaaacgtc ttgaaaagct
     5401 gcaacaattt gatggatttc ctctttatca atgactagag gtggagacaa tcgaatgata
     5461 gtacgatgcg tgtctttgca taagattcca cgttgaatca gttgatccac aaaaggtgca
     5521 gcatctgtgt taagctctat gcctataaat aaaccacgac ctctaatttc tttaatacta
     5581 ggatgtttaa gttgtagcaa cgcttttaat aaaaatgaac ctaagcgttc tgatcgttca
     5641 accagttgtt catctttaag tacatcaagc gctgccgtcg atattgcaat ggctaaaggg
     5701 ttaccaccaa atgttgaacc atgtgtacct ggtgttagaa cacgcatgac atcattattt
     5761 gcaagtacag cagatacagg gtataagccg ccacccaatg ccttacctaa aatataaatg
     5821 tctggaacga cttgctccca ttccatagca aaccatttcc cagttctacc aagaccaact
     5881 tgaatttcat ctgcaatcaa taatatttga tgtttatcac atagttgacg cacagcttga
     5941 atatatcctt tcggtggtat attaacgcca ccttcacctt gaattggttc caaaataatt
     6001 gctgctgtat tcggtgaaat agcttgtgtt aattgttcaa tgtctccaaa atctactgtt
     6061 gtagtgcctt gaagtagggg gtgaaatcct gctttatatg cgtcgtggtt agatagtgat
     6121 aatgagccaa gtgtacgacc gtgaaaattg ttattcatag cgatgatttc aacttgtccg
     6181 tcagtaatgc ctttaacttc agagccccat tttctagcaa ttttaatggc tgcttcaaca
     6241 gcttcagtac cagagttaag ggggagtact ttgtctttct tagcaagatg acaaattttt
     6301 tcttcccatt tcccgagatt gtcactataa aggacacgtg aaatgataga caactttgaa
     6361 gcttgttctg tcatcgcttt aacaattgtt ggatgacaat ggccttggtt tgcaactgaa
     6421 aaacccgaaa tgcaatctat atattgtttg ccatcagtat cccaaacttt gacaccttta
     6481 cctttagaaa tgacaagctt aagtggtgca taattattag agctataata atcagttaat
     6541 tcaatgattg aattcatcgg tctccctcct ttatatttat acattttaaa gaattaatat
     6601 taatatatta cagttgttca atataagtgt caaatgtaaa tttaataata taaagataag
     6661 gttgtgtttg tattaatgtg atttgtaaaa tgaaagtact aatatttttt gtggataatc
     6721 attgatatgt atgtatacga ttgcagttta atgtagtatg aataaaaaat gccacaagca
     6781 tatgtttaac atatgactgt ggcatagtat tatgcttgtg gaattttacg atgcttaatt
     6841 ttataaataa tgaagccgat aatgaaacca atcaaactga gaacaaccca gcccatacca
     6901 atgtctgata atggtaaata tttttggctg aaattaatca aagtttgtga gaatgatgtg
     6961 cttgaaatga actctggact agcttttaat ccatctacta atgcagcaat cattgtaaag
     7021 aaaatggtac attgataaat aagttttgaa tgatggaatt tgctactaaa taatgttagt
     7081 acaatcaagg caattgctaa tggatataag aacattaaca ctgggactga gtacataata
     7141 atcttagtta aaccaacatt cgcgaataag aacgaaataa agcttacaac tgttgcaatc
     7201 gctaggtaat tcattttagg gaaaaggtgt tcgaatgttt ctgaaaatgc cgtaatcaaa
     7261 ccgatggctg tttttaaaca agcaaccata acgataagtg acaacaggac gataccgtag
     7321 ttacctaagt agtattgagt aatttgcgct aaggcaatac caccattttc actaagtttg
     7381 aaatgaccaa tacttaatgt acccatgatt gctagtaggg tataaatgat ccccatcata
     7441 atgatactga tagtaccaga cttaattgtt tctttagcga tatcagttgg attttcgata
     7501 cctaactttt taatcgttgc aacaatgata ataccaaatg ccaatgacgc tagcgcatct
     7561 aaggtattgt atccatctaa aaagccgtta aataaggcat gtgattgata ttgtttacta
     7621 ataggtgcat cagatatgcc acctaatgga tggataaaag caaataataa aataattgct
     7681 aataatacta agaataccgg atttaaaaat ttaccgatat attctaaaat tcttgatggc
     7741 tttctcgcaa aaaaccatgc aatcacaaag aagacgaagc taaaaataaa taaatataaa
     7801 gtgatttgct ttggtgataa aaatggcgaa aatgcaattt caaatgatgt cgttgccagt
     7861 ctaggtaagg cgaaaaatgg tccgataact agatataagg caatcgtgaa aatgtaagca
     7921 tatgttttat taacacgcga tgcaatttca aataaaccag atgtctttga aatgccaata
     7981 gcaatgatac ctagaaatgg taagccaatt gctgtaatta aaaatcctaa gttagcgata
     8041 aaaacgttag aaccagcagc ttgacccaag tgtattggga agataagatt gccggcacca
     8101 aagaataaac caaataacat agaacctata aacatgtttt ctttaaatgt tagtttcttc
     8161 ttcatatatg tatttgttct cctttttaga acttataaaa atctacttaa ttaagataat
     8221 atcaaaaatt aaacataaag tgatacataa ttttgcaata aatactaaat tttttgaaaa
     8281 ttccggaata aatactgttt tttatgtagt aggggcgtaa gttaagaacg atttaacaga
     8341 tatagaatat gttaaatata gaaggttaga caacccgacg tattaatact ttgtgaaata
     8401 aagatccccc tcaacatgat cgaagggggg aagtatatgt ttagttcaat atatttagaa
     8461 agtcgtttgt agttattgtt tgtcccatca atgggaatac attatctatt ggaaattgat
     8521 gtagcgtttc gttttgtgca ctcatcatat ctgtaacaaa aaactgattg tagtttaatt
     8581 gataggcatc tcgcgctgtc gtatctacgc caatatgcgt tgcgacacca ccaagaacaa
     8641 tcgtatcaat tcctcgacgt cgcaattgta agtccaaatc tgttcctaca aatgcactaa
     8701 aatgtcgttt gtctatgaca aaatcgtcat ctctcttgtc taataaatga tggaaacgac
     8761 tgtagtcgtc gccttctttt ggtggtaatg agatcattgc atttggttgc aatacatctt
     8821 taccatcata gaaattcacg cgaacaaaag cgataaagcc attgtttttt ctaaaaacat
     8881 ctattaattt attagcgttt tgaacgacat tttcagctgt atatggggca taatccattt
     8941 taagaatacc ttcttgcagg tcgattaaca ctaaagcggt tttattaaaa ttaatcatct
     9001 tatatcagag tcctattcgt ttaataaagt attcgcttaa acttataccc tgtatacgat
     9061 gaaattattt attttgttgt gaaaaagctt tagcgatatc gatgagtttc ttcggtgcgt
     9121 cttcgacagc cattttgact tcgacaaaat gcatcacatc gggatgacca ttaattgcat
     9181 taaacgtgtc ttgtaaatct tttgatgatt caacgtcatg aatttcaaca tttttaccac
     9241 caaatacagc tggtaaagct ttataatccc acatgtgaat ttcattataa ggttcataca
     9301 tgccgtgaat aagtcgttct accgtatagc cgtcattatt aatcacaaat aataccggtt
     9361 taatatgctg tctaatcata gttgaaatag cttgaacagt tagttgcaat gagccatcac
     9421 caattaataa taagttacga cgatctttgt ctgctaattg tgaacctaat gttgcaggta
     9481 atgtatagcc gatagaaccc cataacggtt gccctataaa agtattgttt ttgtataatg
     9541 ctaaatcata agcaccaaag aatgatgtac cttgatcagc aatgatgaca tcatttggtt
     9601 ttaagaaatt ttgcatcatt ttaaaataag tttgttgtgt taatggttct gtgccaacag
     9661 tataatcggg tgatgttgga cgatgatacg cagggaacgt tgcgttattc gtatatgaaa
     9721 tattggataa ctgttttaac aatgatggta gagatatttc atcatttgta acatcgtcaa
     9781 ttttgatatt gtgatgattt aacataacga catcatcgat attgaattgg tatgaaaaac
     9841 ctgctgttgc tgaatctgtt aatttggctc caatatttaa aattaaatcg ctgttgtcca
     9901 cataatctcg tattttatct tcggcaattt tcccatcgta aatacccata taatatggat
     9961 tttcctcatt aaaagcacct tttcctaatg aaagttgtgc tactggtatc tgtgtttgat
    10021 ttacaaaatc ttctaattct tgatggaggt gaaaactgtt aatttcatgt ccagtaatga
    10081 tgataggctg cttcgcttga tgcagtttag ttgctaataa ctctatatat gttgatgcat
    10141 ccgtatattt agttgccgtc acttcaaatg gtgtcggtat ctcaatttca gagattgcga
    10201 catcgattgg taaatgtaaa tgaactgggc gtctttcggc gattgctgta ttaattaaac
    10261 gtggtatttc ggttgttgca ttttcaggtg tgatataacc ttgtgcaacg gttatatgtg
    10321 caaacatttt tcgatagtcg tcaaatgtac cttcaccaag tgagtgatgt acatatttac
    10381 cgccttgttc aacagcacgt gtcggcgcac ctgtaatcgc aatgacaggt atgcgttcag
    10441 catatgaacc tgcgataccg ttgacggcac ttaattcgcc aacaccaaat gtagtaacta
    10501 atgcagcgag tccattaaga cgggcataac cgtccgctgc gtaacttgcg tttaattcat
    10561 ttgtatttcc tacccaatct acattgggat tgctgataat atcgtctaga aaagcgagat
    10621 taaaatcacc aggaacacca aaaattttat cgacgcctgc tcgatgaata gcgtcaatta
    10681 agtaagctcc aatgcgttgt ttcataaaaa aagccacctt ttctctatat ttacacttaa
    10741 aatgatagcc tcgaatatag tgtaaggtca aggaagttga ctttgttgtg aaaacgctaa
    10801 caatagtgaa caattaatat tttttaggaa atgagtatga aggggggctt taattgatga
    10861 tcttaaaatt caaaatttaa ggggagagcg aactatcaat gatgctgcaa aatacaatag
    10921 caaagaaaaa acaggcgcgt caccttatga aggtatacgc acctgtttat agtaagcatt
    10981 atttagcttc aaataattga tcgccaaatg aaatgttgcc atgttcacct tgtttaaaat
    11041 caaggtttgt aatgtttcct tgtgtcacga taataggcgt aatatcactc tttgcatgat
    11101 tgcggatgta gtctaaatca aagttgatta ataaatcacc ttgtttaact tcttgacctt
    11161 cctcaacatg taaagtaaag ccttctccgt ttaatttaac agtgtctaaa ccgatgtgga
    11221 ttaatagttc taaaccacta tctgatacaa gaccaattgc atgttttgtt gggaaaatca
    11281 tttgtacttt accgttgaat ggtgcacgaa cttcaccttg tgaaggtttg atagcgatac
    11341 cgtcacccat cattttttcg ctgaacactt gatcaggcac ttctgataat ggtgttactt
    11401 caccagttaa tggtgcatgc acgatatggc tcaattcgct tgttgcagat ttatcttctg
    11461 caacaacaac agtttcgtct ttatcgtctt ccatagtagt aggattttct actacttgac
    11521 cattcataat ctgttgcatt tcatgtttga tttggtcaga tttaggacca aaaattgctt
    11581 gcatattatt gccgacttct aatacaccag atgcgcctaa atctttcaaa ccaggaacat
    11641 caactttaga tttgtcgtta acttcaacac gtagacgtgt gatacaagcg tctaaatgtt
    11701 taatgtttgc tttgccaccc atagcttcta atactgcata tggtaattca gttgctgaag
    11761 cagtagccgc ttgtgattgt ttatcttcac gacctggtgt tttgtatttt aattttacaa
    11821 ttaagaatcg gaatacgaag tagtaaataa ctgcgtatac aagacctaca ggaatgacta
    11881 accaccattg tgtcttatta ggtagtatac cgagtaagaa gtagtcgatg aaaccacctg
    11941 agaatgtata acctagatga agatctaata agtacaatgt taagaatgat aaaccatcaa
    12001 gtactgcgtg aataaagaat aataatggtg ctacaaataa gaatgagaat tctaatggtt
    12061 ctgtaatacc agttaagaat gatgttaaag cagcagaacc cattaaacct gctactactt
    12121 tcttattttc aggtttagct gtgtgataaa ttgctaaagc tgctgcaggt aaaccgaaca
    12181 tcataacagg gaattcacct tgcatgaatt taccagctgt caaatgtgcg ccttcacgaa
    12241 tttgttcgat aaagatacgt tggtcaccgt gaataatttc accagctgca tttttccatg
    12301 aaccaaactc gaaccagaac ggtgcgtgga aaatgtgatg tagaccgaat ggaattaata
    12361 aacgcttgat gaaaccaaat aagaatacgg caacaccagt atttgaatct aataatcctg
    12421 tactgaatgc atttaatcct gattgaatcg ttggccaaat taatgccatt gggaatgcta
    12481 aaataaatga tgttgtagcc atcataatag gtacgaaacg cttaccagcg aagaaaccta
    12541 aataagatgg taagttaatg ttatagaact tgttataaca ccaagctgcc agggccccga
    12601 ttataatacc gccgaacaca cctgtttgta atgttgggat acctaaaatg ctagcgtaac
    12661 cactcgctgg atcaccaata ttcttaggtg taacttgtaa aaagtcgccc attgttttgt
    12721 tcatgattat gtaaccgacg aatgctgcga tagctgctac gccatcaccg ccagctaatc
    12781 cgattgcgac acctaatgcg aaaatcatag gcaagttatc aaaaatgata ccaccagcac
    12841 ctgtcattaa tttagcgaca gtttgtacgc caccattttg tataaacggc aagtagtgtt
    12901 gtaatgattc accttgcata gctgtaccga tagctaataa cagaccagct gctggtaaaa
    12961 tcgcaacagg taacattagc gctttaccaa tacgttgcaa ttgaccgaaa agtttcttcc
    13021 tcacttgtcc aacctccaaa gttgtattta ttatttaaga ttcaataaaa aaagacacga
    13081 gcaaatagac tgtgttggta accatcacag cttaatttaa ctcatgccta atcttactta
    13141 gtaacacgtt ggtgtatgta attaatgtaa aagaagcaat tgactaagta gtatataaaa
    13201 ctgatacgtt agtttatcta gcttaccatc acatcttatt gaatcttatt tgtttgcgac
    13261 tcctatttta gcatggtgaa gtatgcgttt tcaatacaat tattaaattc aatttcaaat
    13321 ctcttaattg ttcacagatt attattaaaa cataatataa gataatcctg aataaaatgt
    13381 ataaaagtta ttgtaataat ccagcagtag cagctattat cagcacttaa aagaaaggat
    13441 aatctttgat ggtgtaataa ttaacttgtg acagaaatat gtaagattat tacaatgaac
    13501 aggctttaac tgattaatat tgtaatgtgt tttctcttat agtaaaatgt aagcgattac
    13561 acaactcaac tatattttcc taataattat attgttaagg agggctactt tgacaggctt
    13621 ttcagtgtat ttaggacaac ctttagatga agcgtatatt aagcgaatga ttaaacaagg
    13681 ttaccaaatg atttttacat ctgtacaaat accagaagaa gatgacgaga caaaatatca
    13741 ttatttcaca aaactactca atttattaaa acatgaacaa gtgacttacc tcatagatgc
    13801 taatccatct attttaacac catcttttta tgaccatctt cgacaatatg atgcacaatt
    13861 tatgattcgt atcgatcata gtacatcaat tgaggcaatc gaagcgataa tggcacaggg
    13921 tttaaagtgc tgtttgaatg caagtattat ttcccgggaa ttgttaacaa gcttacatca
    13981 acaattgaat gattttacat tactttcatt ttgtcataac tattatccaa gaccagatac
    14041 gggattatct gttgacttgg tcaataagaa aaatgaactc atttatcaat ttaatccaaa
    14101 ggcacaaata tatggtttta ttgtagggag tgatttgcga ggtcctttgc ataaaggctt
    14161 gccaacaatt gaagcaacga gacatagtca tcctgtcgtt gcagctaaat tattacaaga
    14221 aactggtgta tctgaagtgt tagttggaga ctcattgatt gaaatgaggc aggcaaaaca
    14281 acttatagat ttttgcaagc ataggcattt cacgttatgt attgaagaag tgtttgatac
    14341 gacagtgact taccttttcg atatgtgtca taaagtacgc ccggataatc cggaaaatgt
    14401 cattcgttcg gaaacgtcaa gacaaatatg tccacattcg attcaaccac agtttacgac
    14461 gcaacgacgc attggttcag taaccgttga taatttgaat aacggacgtt atcaaggcga
    14521 aatgcaaatt gtgagacaaa cgcttagtgc acatgacaat gtgaatgttg ttgcacaaat
    14581 tattaaagaa gacttaccac tgttaagttg tatcgagccg aatgatacat ttgattttca
    14641 aaaaactagg gagtgtaaga agtgatggaa aatagtacga ccgaagcgcg taatgaagcg
    14701 acgatgcatc ttgatgaaat gactgtggaa gaggctttaa ttacgatgaa taaagaagat
    14761 cagcaagtcc cgttagcagt tcgaaaggca ataccacaat tgacaaaagt aattaaaaaa
    14821 acaattgcac agtataaaaa gggtggacga ttgatttata tcggtgcagg tacaagtgga
    14881 aggttgggtg tcttagatgc agcggagtgt gtacctacat tcaatactga ccctcatgaa
    14941 attataggta ttattgctgg tggacaacat gctatgacga tggctgtaga aggtgcggaa
    15001 gatcacaaaa aattagcgga agaagatttg aaaaatatag atttaacatc aaaagatgtc
    15061 gttataggaa ttgccgcgag tggcaaaacg ccatatgtta taggcggttt aacatttgct
    15121 aacacaatcg gtgctacaac agtatctatt tcatgcaatg aacatgcagt tataagtgaa
    15181 attgcgcagt atccagtaga agttaaagtt ggtccagaag tattaactgg ttcaacacgt
    15241 ttaaagtctg gtacagcaca aaagttaatt ttaaatatga tttcaaccat cacaatggtt
    15301 ggtgtcggaa aagtttacga taacctcatg attgatgtta aagcaaccaa tcaaaaactg
    15361 atcgaccgtt cagtgcgtat tattcaagaa atatgtgcta tcacatatga tgaagcaatg
    15421 gcgttatatc aggtatctga gcatgatgtg aaagttgcga cagttatggg tatgtgtggc
    15481 atttctaagg aagaagcaac aagacggtta ttaaacaatg gtgacattgt taaacgagca
    15541 atcagagata gacaacctta ggagggattt aaatgaccaa agaacaacaa cttgcagaac
    15601 gaattattgc tgcagtaggt ggtatggata atatagatag tgtcatgaac tgtatgacac
    15661 gtgtgcgtat taaagtatta gatgagaata aagtagatga ccaagaacta aggcatattg
    15721 atggtgtcat gggtgttata cacgatgaac gcattcaagt tgtggttgga cctggtacag
    15781 tcaataaagt ggctaatcat atggcggaat taagtggtgt taaactaggt gacccaatac
    15841 cacaccatca caatgatagt gaaaaaatgg actataaatc atatgcagct gataaagcaa
    15901 aggcgaataa ggaagcgcat aaagcaaaac aaaagaatgg taagttgaat aaagtattga
    15961 aatcaattgc caatatcttt ataccgttga ttcctgcatt tattggagct ggattaattg
    16021 gtggtattgc agcagtactg agtaacttaa tggtggcagg ctatatttca ggtgcttgga
    16081 ttacgcaact tataacagta tttaatgtca ttaaagacgg tatgttagca tacttagcta
    16141 ttttcactgg tattaatgcg gctaaagaat ttggtgcgac accaggactt ggtggcgtga
    16201 ttggtggtac aacgttatta acgggtattg ctggtaaaaa tattttaatg aatgtcttca
    16261 ctggagaacc attgcaacct ggacaaggtg ggattattgg cgttattttt gccgtttgga
    16321 ttttaagtat tgtcgaaaag agattacata aaattgtgcc aaatgcgatt gatattattg
    16381 taacgccgac tattgcattg ttgattgtag gactattaac tatctttatc tttatgccat
    16441 tagcaggttt tgtttcagac agtttagttt cagtagttaa cggaattatt agtattggtg
    16501 gcgtatttag tggatttatc attggtgcaa gcttcctacc gttagttatg ttagggcttc
    16561 atcatatttt tacgccaatt catatagaaa tgattaacca atcaggtgct acttacttat
    16621 tgccaattgc agcgatggct ggtgctggac aagtaggtgc cgcattagca ctttgggtaa
    16681 gatgtaaacg caacacaaca ttacgtaata ctttaaaagg tgcattgcca gttggtttcc
    16741 taggtatcgg agaaccatta atctatggtg tgactttgcc attaggtcga cctttcttaa
    16801 ctgcttgtat tggtggtggt attggtggcg ctgtaatagg tggaattgga catattggtg
    16861 ccaaagcaat aggcccaagt ggtgtgtcac tattaccatt aatctcagat aatatgtatt
    16921 taggttatat tgcaggatta cttgctgcgt atgctggtgg attcgtttgt acatatttat
    16981 ttggaacgac aaaggcgatg cgacagacag atttgttggg tgattaatga tgacaaatat
    17041 tttatatcgc attgataagc agttgagtga ttttacgaag acagaaaaga taatcgctga
    17101 ttacatttta aagaatccac ataaaatcat tgatatgact gtgaatgatt tggcagatgt
    17161 tacgaatgtt agtacagcat caattgttag atttagtcgg aaaatgacac atcaaggttt
    17221 tcaagagcta aagattgcga tatctcgata cttacccgaa gatattgcaa ccaatccaca
    17281 tttagaattg attgaaaatg aatctgtaga aactttgaaa aataaaatga ttgctagagc
    17341 aacgaatacg atgcgatttg tagctactaa tattatggat gcgcaaattg atgcaatttg
    17401 tgatgtgttg aaaaatgcca ggacaatatt tttatttgga tttggcgcat cgagtttgac
    17461 tattggtgat ctttttcaaa agttatctcg tattggctta aatgtcaggt tattacatga
    17521 aacgcattta cttgtgtcaa catttgcgac gcatgatgat agagattgca tgatttttgt
    17581 gacgaatcaa ggtagtcata gtgaattgca gtcaattgca caggtggcca cacattacag
    17641 tattcccatc ataactatat ctagtacagc taataatcca gtggctcaaa ttgcagacta
    17701 tgcattgatt tatggcagaa ctgatgaaaa tgaaatgcgt atggcggcta caacgtcact
    17761 atttgcacag ttattcacgg tagatatatt gtactatcga tttgtagcat taaattatca
    17821 tgcgattcta gattgtataa cccaatcgaa aatggcactt gataattaca ggaagcatct
    17881 tgcgacgata gattttaaac attagatatt gtgtgcgttc taacataaag ttagttgcaa
    17941 cgtaaacaat tgaagttttt gatgtgattc agttgcttga tgaaaaattg ttgctacaag
    18001 tttgtaaaac tggcatagag gctattttga aaatgataaa ggcgtgacaa attaaagcaa
    18061 agttcagatt tgagctttgc ttttttcata gatggaaatt aaatgctgaa gaataatttt
    18121 aatgctaaat gaactaatac gaaggtgcta aaagcaatta ttatcaaatt agtgatatga
    18181 atgaaatgtt taaatcgttt gcgttgattg tattcgggac ttttatagag taggtagtag
    18241 tacataaaga gtatggctgc gaacatgccg aatattgaag tgaagttacc tacagtgact
    18301 gccatgtaat gatttaaagt taaatagtaa cttagactaa taataaaaaa taaaatgatt
    18361 tgataggtaa ttaacaccat tcaagtccct ccattaatcg tagacaaaaa atttatcaat
    18421 gatttgatta tttgtattca aattttagta tacgacttac ctcaaagaca aatattaaaa
    18481 gataagacaa taaaattaga ataaatttac gtttgaagat aaaaaacaaa catttttcat
    18541 taaagtttat gatatattta gagcaataga aagtgtatgg aaggggatat gaatggcata
    18601 ccaaagtgaa tacgcattag aaaatgaaat gatgaatcaa cttgaacaat tgggttacga
    18661 aagagtaacg atacgtgata ataagcaatt gcttgataat tttagaacga ttttaaatga
    18721 gcgtcatgcg gacaaattag aaggcaatcc cttaacagat aaagaatttc aacgtctgtt
    18781 aacgatgatt gatggaaaaa gtattttcga gagtgcccgt attttacgtg ataaattacc
    18841 acttagacgt gatgatgagt ctgagattta tttgtcgttt ttagatacga aaagttggtg
    18901 taaaaataag tttcaagtga cgaatcaagt atctgtcgag gatacatata aagcacgtta
    18961 tgatgtaacg atattaatca acggactacc ccttgtccaa gttgaattga aacgtcgagg
    19021 tattgatatt aatgaggcgt ttaaccaagt aaaacgttac cgcaaacaaa attacacagg
    19081 cttattccgc tacatacaaa tgtttatcat tagtaatggt gttgaaacgc gatacttttc
    19141 taataatgat agcgaactat tgaagagtca catgttttat tggagtgata aacagaataa
    19201 ccgtatcaat acattgcaat cgtttgctga gtcatttatg agaccttgtc aattagctaa
    19261 gatgatatcg cgctatatga ttattaatga aacagataga atactgatgg caatgcgtcc
    19321 gtatcaagtg tatgcggtag aagcacttat tcaacaagcg actgagacag ggaataatgg
    19381 atatgtatgg catacaactg gaagtggtaa gacgttgact tcttttaaag cgagtcagat
    19441 tttatcacag caagatgaca ttaagaaagt tatctttttg gttgaccgta aagacttgga
    19501 tagtcaaaca gaagaggaat ttaataaatt tgctaagggt gctgtagaca aaacttttaa
    19561 tacctcgcaa ctggtacgcc aactaaatga taaaagtttg ccacttattg taacgacgat
    19621 tcaaaaaatg gctaaagcga ttcaagggaa tgccccttta ttagaacagt ataaaacgaa
    19681 taaagttgta tttattattg atgagtgtca tcgcagtcaa tttggtgaca tgcatcgtct
    19741 agttaaacaa catttcaaaa atgcccaata ctttggattc actggtacgc cacgttttcc
    19801 agaaaatagt agtcaagatg gtagaacaac tgcagatatt ttcggtagat gcttacatac
    19861 gtatttaatt agagatgcca ttcatgatgg taatgtactt ggtttctcag ttgactatat
    19921 taatactttt aaaaataaag ctttaaaagc agaagataac agcatggttg aagcaattga
    19981 tacggaagaa gtatggttag cggataaacg tgtggaatta gtaacacgac atatcatcaa
    20041 taatcatgat aaatatacac gtaatcgtca atattcaagt atatttacag tccaaagtat
    20101 tcacgcgctt attaaatatt atgagacatt taagcgactt aacaaaaagt tggaacaacc
    20161 gttaacgata gctggtatat ttacgtttaa acctaatgaa gatgatcgtg atggtgaagt
    20221 gccatatcat tcacgtgaaa aattagagat aatgattagt gattataata aaaagttcga
    20281 gacgaatttt tcaacagaca caactaatga gtattttaat catatttcaa aaaacgttaa
    20341 aaagggcgtt aaagatagta aaattgatat cttaatcgtt gttaatatgt tcttaactgg
    20401 ttttgatagt aaagtactga acactttata tgttgataag aatttaatgt atcatgattt
    20461 aattcaagcg tattcacgta caaatagggt tgaaaaagaa tcaaagccat ttggtaaaat
    20521 tgtaaactat cgtgacttga aaaaagagac agacgatgca ctgagagtat tctcacaaac
    20581 aaatgatacg gatacaattt taatgcgcag ttatgaagag tataaaaaag aatttatgga
    20641 cgcttatcgt gagcttaaaa tgattgtgcc gacaccacac atggttgatg acattcaaga
    20701 tgaagaagag ctaaagcgct ttgttgaagc ttatcgttta ttagctaaaa taatattacg
    20761 tttaaaagca tttgacgagt ttgagtttac aattgatgaa attggaatgg atgaacaaga
    20821 gaatgaagac tataaaagta aatatttagc tgtgtacgat caagtaaaaa gagcgacggc
    20881 tgagaaaaat aaagtatcca ttttaaatga tattgatttc gaaatagaaa tgatgcgtaa
    20941 tgatacgatt aatgtgaatt atattatgaa tatattgaga caaattgatc ttgaagacaa
    21001 agcggaacaa cgtcgtaacc aagaacaaat tagacgcatt ttagatcatg cagatgatcc
    21061 gacattgagg ttaaaacgag atctaattag agaattcatc gacaatgttg taccttcttt
    21121 aaataaggat gatgatatcg atcaagaata tgttaatttc gaaagtatta aaaaagaagc
    21181 ggagttcaaa ggatttgctg gagagagatc tatcgatgaa caagccctaa aaacaatttc
    21241 aaatgactac cagtatagtg gtgttgtaaa cccacatcac cttaaaaaaa tgattggtga
    21301 tttgccattg aaagaaaagc gtaaagcaag aaaagccatt gaatctttcg tggcagaaac
    21361 aactgaaaaa tacggtgtgt aatgattcag ccccctcgct agattagtgt agggggcatt
    21421 attattttct atcttttgtt agttaccatg tgtttggcaa ccatcatttg gtttcaatac
    21481 aattttataa tcgtatgtgt atagaaataa tgtctatagg catttaaaat agtttataat
    21541 attaacagga taagtattaa atgtatgttg aaggaggtgc gtagctatgg ataatagaaa
    21601 tatgattaat cgtgttttta gtcaaaagat attacatcaa attgcaatca aaaataaaag
    21661 tgatgttgtt gatgaggcat atgattttta tatacaggga cctaaaaata tcaatgtaat
    21721 acagaagatg aaatctttat ataactatct taaaaagtct tatcgtaacg aatattttta
    21781 caaaaacaca atacttaata aactccttct aggacgacat tctattaata caactactgc
    21841 actttctgag atgcccatag ggaaaagtat tgctgatttt atattgttaa acggcaaagg
    21901 cgttgtctat gagattaaaa cagaattaga taagctggat agattagata atcaaattaa
    21961 tgattattat gaagtgttta attatgtcgt agttattaca aatgacaaac atctgaataa
    22021 agttatggct agatacaaag atacaacagt tggaatttta gtgttaacaa ctagaaatac
    22081 actgagtgaa gttcaaaaac ctaaagaaaa caatagtctc ttaaacacaa aagcgatgta
    22141 caacttttta cgaaaagaag aaagaaaaag agttattgca caaaatcata tggatgtgcc
    22201 aacttataat gatttcacag agtatgatgt gttatttgac gtgtttaaag aaataccaat
    22261 gacgaaactg cataacaata tgatttctga gttgaaaaaa agaggaaaca tgaaagaata
    22321 caaagatgaa tttttagcag cgccggctga aattaagttc ttgttatatt tcgcaaaaat
    22381 gacaaagaaa gataaaaata aactatatca ttttcttaag gaggattaat atgtattatc
    22441 cttatttgcg tgggaaacaa aatgaactat ttgcaattag agaattgtta gagaaaggtt
    22501 tgattggtga ttgtattcaa cctataattg aaccaattaa atatacaacc acatttaaaa
    22561 atactttgca atactgtggt gaaaaagcat tctctataaa tttagtagta aattcaaagt
    22621 taactgaaga agagattagt aacgaaactg ttgcacattt aactgaaata ataacaaaaa
    22681 acaaaagtgt tattcaaaaa gcttacttgg gtccttctga tgaaggcaat gataggttga
    22741 aacagcaatt ttcaagtaat agtttagcta ttttaacaag tgtagatgat tgggaaatgt
    22801 ttggagataa aaataaactt gaaatggttt ttgtaccaga tgatagacac attaaacgta
    22861 aattgcgtaa tattccaaac aaaggcatca ttatggatcc ttttaataaa ctaagtcgta
    22921 atgttgatta tttagataat gatgacgagt tttatagcga cgatcacctt tattataagg
    22981 aagatggata cgtagcattt tcagactatt ctgttatagg tggagaatat gtagacggtg
    23041 gcttttcgcc attagctatt gcgatacata ttgtctattt tgatgaggct aatgagctaa
    23101 gagttaagca ttttgtctct gattctaata atgatagatc aaatccaggt aaaaagtttt
    23161 ttgaggctgt agataaatta gtaacatggt caaaaaactt agatattaaa aatagatctt
    23221 atgcgcttgg acaatttgaa gaattaaatg aaaataataa gtatccagga ttaggtttaa
    23281 ttaaaaggtt atctatcatg catcacctag aaattatgaa tagatacttg gagtctcaaa
    23341 atgaaaatat gtgaaaaatg ttttaataat actgaaatcg tagaaatcat tgcaaatgat
    23401 aatagcaaat ttgacaattg tgatattgat aacaatcatc ttggtgttaa aatatttgat
    23461 a
//
LOCUS       DANRDI010000027        23189 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.027, whole genome shotgun sequence.
ACCESSION   DANRDI010000027 DANRDI010000000
VERSION     DANRDI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.027"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..934)
                     /locus_tag="PZ377_000819"
     CDS             complement(<1..934)
                     /locus_tag="PZ377_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDM6357705.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGA"
     gene            complement(947..3577)
                     /locus_tag="PZ377_000820"
     CDS             complement(947..3577)
                     /locus_tag="PZ377_000820"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDM6357706.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(3621..3922)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3980..4540
                     /locus_tag="PZ377_000821"
     CDS             3980..4540
                     /locus_tag="PZ377_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDM6357707.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     gene            complement(4763..5836)
                     /locus_tag="PZ377_000822"
     CDS             complement(4763..5836)
                     /locus_tag="PZ377_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDM6357708.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(5870..5971)
                     /locus_tag="PZ377_000823"
     CDS             complement(5870..5971)
                     /locus_tag="PZ377_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357709.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(6032..7318)
                     /gene="hemL"
                     /locus_tag="PZ377_000824"
     CDS             complement(6032..7318)
                     /gene="hemL"
                     /locus_tag="PZ377_000824"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDM6357710.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(7366..8340)
                     /gene="hemB"
                     /locus_tag="PZ377_000825"
     CDS             complement(7366..8340)
                     /gene="hemB"
                     /locus_tag="PZ377_000825"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDM6357711.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(8343..9011)
                     /locus_tag="PZ377_000826"
     CDS             complement(8343..9011)
                     /locus_tag="PZ377_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDM6357712.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(9033..9959)
                     /gene="hemC"
                     /locus_tag="PZ377_000827"
     CDS             complement(9033..9959)
                     /gene="hemC"
                     /locus_tag="PZ377_000827"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDM6357713.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(10001..10816)
                     /gene="ccsA"
                     /locus_tag="PZ377_000828"
     CDS             complement(10001..10816)
                     /gene="ccsA"
                     /locus_tag="PZ377_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDM6357714.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            complement(10838..12184)
                     /locus_tag="PZ377_000829"
     CDS             complement(10838..12184)
                     /locus_tag="PZ377_000829"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDM6357715.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(12401..12991)
                     /locus_tag="PZ377_000830"
     CDS             complement(12401..12991)
                     /locus_tag="PZ377_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDM6357716.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(13145..14407)
                     /gene="clpX"
                     /locus_tag="PZ377_000831"
     CDS             complement(13145..14407)
                     /gene="clpX"
                     /locus_tag="PZ377_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDM6357717.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(14558..15859)
                     /locus_tag="PZ377_000832"
     CDS             complement(14558..15859)
                     /locus_tag="PZ377_000832"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDM6357718.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(15907..16017)
                     /locus_tag="PZ377_000833"
     CDS             complement(15907..16017)
                     /locus_tag="PZ377_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357719.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(16022..16951)
                     /locus_tag="PZ377_000834"
     CDS             complement(16022..16951)
                     /locus_tag="PZ377_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357720.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(16970..17578)
                     /locus_tag="PZ377_000835"
     CDS             complement(16970..17578)
                     /locus_tag="PZ377_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDM6357721.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(17720..18076)
                     /gene="rplT"
                     /locus_tag="PZ377_000836"
     CDS             complement(17720..18076)
                     /gene="rplT"
                     /locus_tag="PZ377_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDM6357722.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(18123..18323)
                     /gene="rpmI"
                     /locus_tag="PZ377_000837"
     CDS             complement(18123..18323)
                     /gene="rpmI"
                     /locus_tag="PZ377_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDM6357723.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(18352..18879)
                     /gene="infC"
                     /locus_tag="PZ377_000838"
     CDS             complement(18352..18879)
                     /gene="infC"
                     /locus_tag="PZ377_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDM6357724.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(18908..19033)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(19108..20601)
                     /locus_tag="PZ377_000839"
     CDS             complement(19108..20601)
                     /locus_tag="PZ377_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6357725.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYKYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(21027..22964)
                     /gene="thrS"
                     /locus_tag="PZ377_000840"
     CDS             complement(21027..22964)
                     /gene="thrS"
                     /locus_tag="PZ377_000840"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDM6357726.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
BASE COUNT         7220 a         4500 c         3176 g         8293 t
ORIGIN      
        1 gtgcgccatc caaaatgatt aaaggtttgt catgcacctg ctcaatacgt ccagtccaac
       61 gaactgattc aataccgtct atcatcttat tgaaatctaa ttcaattaat ccttgttcat
      121 ttaattcaat aagagctgtt atggctaatg cagcattttg tttctgatgt tcacctaaca
      181 tgcttaaaat gattgtttct aattcataat ctttataacg gtaagtaaat tcatcatttt
      241 gcgatacaac aacaatttct ctatctaatt caattggctt tgcatgttgt tcaattgcgc
      301 gttcacgaac atattttaat gcatcttcat ttttaacagc atatatcact ggaacgttag
      361 gctttataat cgcgccttta tccctagcaa tatctagata agtaccacct aaaatatctg
      421 tatggtctag accgatacta gttaagattg ataaaaccgg tgtaaagaca tttgtcgaat
      481 cgttctttat acccaatcca gcctcaacaa tgacaaaatc aacaggatgt atttcaccaa
      541 aatataaaaa catcatcgct gtgattattt cgaattcagt tgcaacacct aaatctgttt
      601 cacgttccat catttcactt actggtttaa tacgtgatac taattctaca atagcgtcat
      661 ttgatattgg cacaccattt agactaattc gttcattaaa tgtttcaata aacggcgacg
      721 taaatgtacc tacttcataa ccattttcaa ctaaagctgt tctaaggtaa gcaactgtag
      781 agcctttacc atttgtgcca cctacatgaa tacccttaat gttattttga ggattattaa
      841 attgtgctag catccattcc atacgtttaa cacctggttt gatgccaaat ttagttcttt
      901 cgtgtatcca atacaagctc tctaggtaat tcattgttac taactcctat gcttttaatt
      961 gttcaattct tgccttcaca ccatcatatt tttcttgata atcttgtttt ttacgttttt
     1021 cttcatttat aaccttttca ggtgctttac ttacaaagtt ttcattagag agctttttat
     1081 ctactctatc taattcgctt tgaagtttag ctaattcttt ttccaaacgg ctgatttcct
     1141 tatccatatc aattagccct tctaatggta ataccacttt acctgcaatt acaactgatg
     1201 tcattgcttt ctcaggaatt tccacgtcag tgctaatatt taaggtacta ggattacaga
     1261 atttgattaa ataatctttg ttttgtgata aagttgtttc aatttcttta tctttagctt
     1321 gaattaaaat aggtatttct ttagacaatg gcgtatttac ttctacacgt gattgtctta
     1381 cagatttaat gatttcaaca agttgttgca ttgtttgttt actttcttca aaaatcaatg
     1441 attcacgcac ttctggccat gaagctttaa caattgtgtc accttcatgt ggtaaacttt
     1501 gccatatttt ctctgttaca aatggcatga atggatgtag cattctcata atattgtcta
     1561 aagtataact caatactgaa cgtgtaactt gtttttgttc ttcatcatta ctattcattg
     1621 gaattttact catttcaatg taccaatcac agaaatcatc ccaaatgaaa ttatataatg
     1681 cacgtccaac ttcgccgaat tcatatttgt cacttaaatc agtaactgtt gcaatcgttt
     1741 catttaaacg tgttagaatc catttatctg ctaatgataa gttaccactt aaatcgatat
     1801 cttcaacttt aaagtcttca ccgatattca ttaaactgaa acgtgcccca ttccagattt
     1861 tattgataaa gttccacact gactcaactt tttcagttga gtatcttaaa tcatgtcctg
     1921 gagatgaacc tgttgctaag aagtaacgca agctatcagc accgtattcg tcaataacat
     1981 ccattggatc cacaccatta cctaatgatt tactcatctt acgcccgtct tcagcacgaa
     2041 ctaaaccgtg taataataca tcattaaatg gacgacgatc tgtaaattct aagccttgga
     2101 atatcatgcg tgctacccag aaaaagataa tatcgtaacc tgtaactaag gcatttgttg
     2161 ggtagtatcg tttaaagtct tcactttcta aatcaggcca acctaacgta gagaaaggcc
     2221 ataaagcact tgagaaccac gtatctaata catcttcatc ttgttgccaa ttttcaatat
     2281 cagttggcgc ttcttctcca acatatattt cgcctgtttc tttatgatac caagccggaa
     2341 tttgatgacc ccaccataat tgtcttgaaa tcgtccaatc tctaatattt tccatccatt
     2401 ggttaaatgt atgttcgaaa cgttgcggat aaaaatcaat acgatcatct gttttttggt
     2461 tatctaatga acgtttcgct aagtcttcca tgcgcacaaa ccattgtgtt gataaatatg
     2521 gttcaacaac agcgccagat cgttctgaat gacctacaga atgaacatga tcttcaatct
     2581 tgataactaa atcttgttct tttaaatctt taactagctg tttacgacaa tcaaaacggt
     2641 ccataccttc atatttaccc gctttgtcgt tcattttacc attttcatcc ataacgataa
     2701 tattttctaa ttgatgtctt tgaccaattt caaaatcatt agggtcatgt gctggtgtca
     2761 ctttcatagc accagaaccg aagtctatat caacatactc atctgctaaa ataggcagtt
     2821 cgcgtcctac gattggtaat ataacagttt taccgattac atctttgtat cgttcgtcat
     2881 tagggttaac aacaatcgct gtatcaccta acatcgtttc tggtcttgtt gttgcaattt
     2941 caataaaacc ttcaccatca gcgtaaggat atttaaaatg ataaaacgca ccttgaacat
     3001 cttcatgtat tacttcaata tcagataaag ctgtacgtgc tttaggatcc caatttataa
     3061 tacgttcgcc acgataaata attcctttat tgtataaatc aacaaaaact tttttaactg
     3121 ctttacttaa accttcatct aaagtaaaac gttctctact ataatctaaa cctagaccta
     3181 atttagccca ttgcgcacga ataaatgacg catactcttc tttccaatcc catgcctgtt
     3241 ctaaaaactt ttcacgacca agatcatatc tagttattcc ttgttcattt aatttagctt
     3301 ctacctttgc ctgtgtcgca ataccagcat gatccatacc tggtaagtat aacgtatcgt
     3361 atccttgcat acgtttcata cgtgtaatga tatcttgtaa agtcgtatcc catgcatgtc
     3421 ctaaatgtaa tttaccagtt acatttggtg gcgggataac aattgtatat gtttcttttg
     3481 atttatcttc tgacggttta aaataaccat tctttaccca ttcttcataa cgtcccgctt
     3541 caacttcacg aggatcatat tttggtttca tttccatttg ctatacctcc taaaaaaata
     3601 aaaatatcca tcctatatac aaataggacg gatattccgt ggtaccacct atattcaaga
     3661 aggatgatta atatcaaatt cactctttta acataattgg aataatcata ccaatactat
     3721 catcgtgaaa tttgaaatgc ttcatctctt caagcactct agattatgat taacgctcaa
     3781 acacgtctta gcctactatt aatcacgttc agctaagata ctctgtgggc taccttcagt
     3841 aaaaatcatt tacatactca caccaaatca tatgctctct ttaaaataat ttgaacttac
     3901 tcttcccaaa tcctatatta aactcttaac ttatagtata atgattgaca aaataagtca
     3961 atgtataggt gggaataaaa tgaatgaatg cgcatttggt actaaagatc cagtctactt
     4021 agactatcat gatcatgtat ggggacaacc gctctatgat agcaaggcat tgtttaaact
     4081 tttagcatta gaatcacaac atgctgggct atcttggtta actattttaa aaaagaaaga
     4141 agcctatgaa gaagcatttt atgatttcga accagaaaag gtagcacaaa tgaccgctca
     4201 agatatcgac cgcttaatga cttttccaaa tatcgttcat catcgtaaaa aattagaagc
     4261 aattgttaat caagctcaag ggtatttaaa aattgaacaa gcatatggta gttttagtaa
     4321 atttttatgg tcatatgtaa atggtaagcc taaagatttg cagtatgaac atgcttctga
     4381 tcgtatcaca gttgatgata ctgcaacaca actatctaaa gatttaaaac aatacgggtt
     4441 taaattttta ggtccagtaa cagtattttc gtttttagaa gcagccggtt tatatgatgc
     4501 acatttacaa gattgtccat caaagcctaa acataattaa tttgctatag catcataaag
     4561 taaacgagac atcatctatt tttaactaat agactgtctc gttttttaat gtgaaaattt
     4621 attcaaatca taagttaaag ttccaagtta atgctctgct aatattagat atcttaatct
     4681 aaaaatagat gtacaacctt tgcttaaacc ttaataatga attctatcct aagcataatt
     4741 caacttagat ggatgatagt ttctaatctt cacgcttatt cgaacgatat tttaaaaagt
     4801 aattaattcc tggtgcaata ataaatagga tgaaaaagat tcggaaaata tgataactcg
     4861 taatcatagc aacatcggca ccagtagcta atgcaactaa aactatctga ttaacccctc
     4921 ctggtgctgc accaagaaac aattcattaa taggattatt atcaaagaaa tgtatgatat
     4981 aaaccatgat tagcgcacca attatcaaca taatattttg aattgtaatt gcgattgcta
     5041 gtctaccttt taaatctgac aataaatgcg caatttgaac tccaattcta atcatatata
     5101 ttagttgtgc catgttcaac aaccaatgat ctagtgtaaa tgttaaacct gtagaaaaat
     5161 tccaaacaat taatacaatg agtggtgcta ataattgaaa tgttggaaac tttattttag
     5221 acataattag ataaactata aagatagcta tcgctaaaat aactatttgc cctatgttta
     5281 atacttgtga taaaggcaag acttttgtta actttccatt cgcatgcatg ttaccatcat
     5341 gaaaaaaata tgaaatgaac ggtactaaaa caacaacaaa tataattcgt gatgtttgcg
     5401 ttaagctaac aactaacaaa ttagcacgtt tgtcttgttc agccatgacc agcatttgtg
     5461 ttagtgctcc tggtataaca cttaaaatag ctgtttctgt attaatacgt gcaatttttt
     5521 taaaaacaaa tgccattact attgcaatta ataatatcga aatagataca acaataatcg
     5581 aaagccaatt gtttttaata tccataacga catttttcgt aaacgttgat ccgatttgca
     5641 cacctaatag tacaatacct aattcactaa gtaagaatgg ccatttaata tcaagtttga
     5701 aaacttttac acaaatgatt gatgcgataa taggaccaaa cataaatgga agtaatacgt
     5761 gcgatgaata cagtagaata ctaataaaaa atgataaaac gaacacaatg aaattatttc
     5821 tatatatcat tgccatgttt tccacttctt tcaataaaaa ataaaatgac taaattgctg
     5881 cttgagcttc acgtttgtta agataacaat atccgctagc agttttgact acaaagcata
     5941 tatggacttt cactatcaag tcgccgccca tgccttatat acatttaaaa agagcctgaa
     6001 caaagttcag gctctcaatt tgtccgtata tttattttac aatacgactt aaagccgtat
     6061 caaatgcttg aatcgttttt tcaatatctt ctttcgtgtg tgccgtagat aagaatgtac
     6121 cttcaaattg agatggtggt aaaaacacac cttcttttgc catttctcga tacatttctg
     6181 caaataattt caaatcactt ttattcgctt gttcaaaatt agttacaggt ccttcattta
     6241 agaaataacc aatcattgaa cctgctctat ttacagttat tggtacattg tgtttagcaa
     6301 atacacgttt taaaccgtct tcaagtatat cgcctaacat attaaaatac tcatatgtct
     6361 ctggcgttaa ttggcttaac gtttcataac cacttgtcat tgcaagagga tttcctgata
     6421 acgtacccgc ttgataaata tttcctaatg gtgctatatg atccatgatt tcttttttac
     6481 caccaaatgc acctacaggt agtcctccac cgataacttt tcctaagcaa gttaaatctg
     6541 gtgtcacacc aaagtaacct tgtgcacaat gataaccgac tctgaaacca gtcattactt
     6601 catcgaaaat tagcaatgcg ccgtattcag tcgtaatatc tcttaatccc tgtaaaaaac
     6661 cttcaatcgg cggtacgaca cccatattac cagcaacagg ttctacgatt acaccagcaa
     6721 tatcgtttcc aaatttttcg aaagcgattt taagtgcatc taaatcattg tatggaactg
     6781 taattgtatt tttagcaata ccttcaggca caccaggaga atccggcaat cctaatgttg
     6841 ccaccccaga accagctttg attaataacg aatcactatg accatgatag caaccttcaa
     6901 atttcacaat tttatttctg ccagtataac cacgtgctaa tcttaaagta tccaatgtag
     6961 cttctgtacc agatgacacc atacgcactt tttctattga aggtactcgg tcaataacga
     7021 gctgcgccaa tttattttca agtaatgttg atgcaccaaa acttgtacct ttatcaattg
     7081 cttcatgtaa atgactaata acttgagggt ctctatgtcc taaaataagt ggtccccaac
     7141 ttagtacata gtcgatatac tcgttaccat cgatatcata aatttttgaa cctttaccgt
     7201 gatccataaa aattgctggt gtatctactg atttaaatgc gcgtactgga ctatttacac
     7261 caccaggcat taaagtttca gcaaccttca ttgcttcttc tgatttcgta tatctcataa
     7321 agtaaattcc tcctacagtt tatggaaaat cataaatata aaaccttatt tatctaaata
     7381 gcgacaaatg tcctttgcaa aatacgtaat aatcatatca gcacctgcac gtttcattga
     7441 aaccatttgt tccataacga cacgttcttc atctatccaa ccattttgtg ccgctgcttt
     7501 agtcatacta tattctccac tcacattata tgcaacaact ggaacattcg tatgattttt
     7561 aacatctcga actatatcta aataacttag agcaggttta acaatcatca tgtcgcaccc
     7621 ttctttaaga tcactttcta attcacgaag tgcttccaaa cggttagcag ggtccatctg
     7681 atacgttttt ctatccccaa atgatggcgc tgaatctgct gcatctctaa aaggtccaaa
     7741 gaaacttgat gcatacttga caccataact cattatagga atattgtaat agccggcttc
     7801 atctaatcca cgacgaattt cagcaacaaa accatccatc atattacttg gcgcaataat
     7861 atcagcacca gcttccactt gagaaattgc tgttttaaca agtagtggca atgatttatc
     7921 attgtcaacg tcatgtgtat ggtcatcaat cacgccacaa tgaccatgat cagtatattc
     7981 acataaacaa gtgtctgcaa caattaataa gtcatcatac atttttttag caatacgtgt
     8041 tgcctgttga ataacaccat cgtgaatgta tgcaccagta cctatatcat cttttgagtt
     8101 tggaacaccg aaaaacataa tggcacgtat gcctaagtca taagcttctt ttaattcact
     8161 ttcaagtaaa ttcaaactga tttggtatac acctggcaat gacttaattt cttttttcac
     8221 atcgtctttt tcaactacaa aaattggata tattaaatct tcttttctta catgattctc
     8281 tctaaccata tctctcattg tcgctgatga tctcaatctt ctatgtctat caaatttcat
     8341 ttttagcccc tactttctaa aatcttttca attagtgatt cgagtgtttg aatttctgca
     8401 attgttactg gttgttgata tgatttaatg gtccgtgctg tttgttctcc aatagcaaaa
     8461 tacgacttga attttggtac aaatccttca ttaaaataat aacgtactgc cgacgaactt
     8521 gaaaatgtta atgcatcgat ttgttgatgt tctatcattt ctttaacatc ttgtatattt
     8581 tgtttgttag gcactgaagt atataaatct attttaacaa cttcattatc tttagataac
     8641 gctgctaata acaatggtct cgccaattca ctcgaaggca aaagtatttt ttggttagtt
     8701 tgattaaatg attttaaaaa tccttcttga gaaaagtcgt ttggcataaa atcaactcga
     8761 atgccaagtg attcacaata ttgcgctgtc ttacttccta tcacagcaat gttatcaaca
     8821 ttaattcctt ttagatattt ataaaagaat ttcacagcat ttttagatga aaaaataagc
     8881 cagtcatagc gttgatttaa caaatgaata tcaaaattta gtggctttat atcaataaag
     8941 ggtttgtgaa taattgatac taaatcactt tgcatgtcat ttgtttgtgt cataactaca
     9001 actggcttca tatttaaacg tcactccatt atttaatgtt gttcatttaa gcgttttata
     9061 atttcataag caccttgctc ttttaatttg ttactcactg ttttgcctaa ctcaaccgga
     9121 tctgttccgt tcattgtata ttcaaatcgt tctttaccat ctggggtcat aattaaacct
     9181 gtaaattcga tttcgttttg atctgagatt gtagcatatc ctgcaattgg cacctgacaa
     9241 ctaccatcca tttctgctaa aaacgttcgt tcagcagtca cacattttgc aacctcatca
     9301 ttatgtactt tgcttaataa tgttaatagt tcttcatcgt cactacgaca ttctatccct
     9361 aaagcacctt gtccgattgc aggtaacaat gtatctctat caagataaga tgttacaata
     9421 tcatctgacc agcccattct tcttaaacca gctgcagcta aaataatcgc atcataatct
     9481 tcagtttgta acttttctaa tcgtgtatct atattacctc taatccattt aatctctaaa
     9541 ttaggatact tagataatat ttgtgcacca cgacgtaatg aactagtacc aataatactg
     9601 ccttctggca attgggatag tggtgtatgt gttttagaaa tatacgcatc aaaaggtaat
     9661 tctctatcag ggatacaacc taatgttaaa ccttccggaa ttacacttgg tacgtcttta
     9721 agcgagtgta ttgccatatc gatatttttt tcaaaaagtt catgttgtat ttctttaaca
     9781 aataagcctt tgcctccgac tttagacaat tgtttatcta ctatacgatc gcctttcgtg
     9841 acaatttctt taatttcaat ttctagattt ggctcgacag cttttaattt atcaataaat
     9901 tgctggcttt gtgttaaagc taatttactt cttctggagc caacgactaa tttacgcatg
     9961 ttcaattcct cctaggaacg gattgctcta gattattttc tcaattcaca aaatgtgttg
    10021 caaaaaataa attaatcata tttaagcaaa ataaaataat gttatagtat attaaatatc
    10081 ttgaattcaa ccatttgttg attctaagta aaatataact tccatataat actgtaataa
    10141 ttgaagagag tattaccttc gggtcaatga atatacgttc accaactgaa attacacccc
    10201 actgtgtacc taaaataata ctaaatatga gaattatcca cccacttaac gttgagtaaa
    10261 acacaattga ttcaagtgta gcaacgctac caattctaaa gtatttttga tcaaaacgtt
    10321 tttccttcaa attacggtat tgcatgatat acagtaatgc attgacaaaa gctaaggcaa
    10381 agaagacata acttaacaca gctagaccga tatggactaa cagtaactcg tctacaacag
    10441 caattttctg aaccttatta gtataatgtg tcggttgaaa tgtattcatc cctaaaagtg
    10501 ttaaccctat taaattcaag aaaaacacag agaaattcaa aactttgata aggttaagaa
    10561 ttaaagagat tgaaataatc agccaactta aagtatagaa tacatctgaa atagacccta
    10621 atggaatatg tctagtttgt ataataaaaa tagataaaga gattgtttgt aaaacccaaa
    10681 caatccccaa taaatatatg cctaaacttc taatcttatg acttttttgt acaaaatcat
    10741 aaaaatagca aatgatactg attaagtata ttaataatat aatttcattg aatcgaataa
    10801 acaggttttc ttgcatatta tcaccatatt aatatgctta ttcaaaacta aagatacgtc
    10861 gcgctgaaat ttccttgacc ttgctttctt tttgttgctt cgcttgctca tgaggacatt
    10921 cagcttcgat atcaaatata ttttgaaata gctctaattt ttcattactt ttcttatcac
    10981 tacttaattc tttggcctgt ttaataggat ctttcaacat ttgattgatg atactttttg
    11041 tatgtttaga tataatttta cgttctcttt cgcttaaccc tggcaattta cgatcaatac
    11101 tgtccatcgt ttctgcttga attgccatag ctttttcacg taaagctcta atcactggaa
    11161 caacacccaa catactaatc cactcattgt gtgcatgtat ttctgcagga atttgttccg
    11221 aaattgttgc agccgctaat tgtcgctcac gtaagtttgc atcaactaaa ccttttaagt
    11281 catcaacatc ataattaaag atgtttgtga tggcactaat accaggttca atatctcgag
    11341 gaactgcaat atcaatcaat actagtgaat cttgctttct attttctgca attctttcta
    11401 tcatttcatt tgtaatgata taagattgtg cactcgttga actaatcaca atatctgcac
    11461 tttcaagtaa atttggtaat gatgatagtt catcatattt cacttgatgc tttgctgcta
    11521 atttcatagc attttcaatt gttctattta ctactgtaat atcagtaatt ccagaaccaa
    11581 gaagatttaa tagtgataat tcactcattt cccctgcacc aataatgata gcttgcttac
    11641 ttttcaattt gccaaatact tttttcgcca actcgaccgc agcataagac acacttacag
    11701 cattatcagc tatatctgtt tcattatgtg ctctttttgc aaaagtaatt gcctgtttaa
    11761 atagatgatt aaaaattgtt cctgtcgtac ctgtgctttg cgctaagaaa aatgcatctc
    11821 ttatttgacc taaaatttga gtttctccaa gtacgattga atctaaacca gaagtgacac
    11881 gcaataaatg ttctactgct tcgtccccca cttttacttc tgacattgct ttaatatcat
    11941 ctacttcaaa tccaaatgca cgagctagaa atcgttgaat atagtaacga cctgtgtgaa
    12001 tttgatcaac aacagcatat acttcagttc gattacatgt tgataatatg acattttcta
    12061 aaatagattt agtttcatat aaatcttcat gggcaattcg taaggcatca tctctaaaag
    12121 caacttgctc tcttagtgca acatcagctg tgcgatgatt tatactaatt gcaataaaat
    12181 gcatttgaaa cgcccccata tatactaaca atacaaatat tataacataa tttaaaatcc
    12241 aattaagtac gatgcttaaa taaattataa tctttctaat ttacttttga gtttttgaca
    12301 aaatgatgaa cgttgaaaaa tgttatcaat tgtgtactaa ttaaaagttt cgtgcttaca
    12361 ttatttttaa aatttttgta taagttttga cgtacaaaaa ctatgaaata tacggttcaa
    12421 ttaaattcca tatttgttgt tgtttattat tttgaattga tgaataactt acaattgtat
    12481 cgtctgggtc catatctaat tgtgtcttaa tatttttaat atgcttttga accttacctt
    12541 ttggaatttt gtcttcttta gtgcatataa ctaaagtagg aatatcaaaa tgtttcaaat
    12601 aattgtacat taagatatca tcttgtgttg gatcatgtct taaatcaact aattgaataa
    12661 ctaattgcaa attctctctc ttagttatat attcctcaat cattttccca aatttttcac
    12721 gttgtgtttt acttacttta gcatatccat accctggaac atccacaaaa ataagttgtt
    12781 catctatatt ataaaaattt aacgtttgcg ttttgccggg ttgctgtgat gtacgtgcca
    12841 tattttttct gccaatcata ctattgataa atgtagactt acctacatta gatcgaccgc
    12901 tcagtgcaac ttcagacaat tctgtttctg gatattgttc ttcttttact gcactaatga
    12961 ttaattctat attattagga ttaactttca ttttatatcc tcactttttt atattctctt
    13021 tcaattctac cattatataa tatttgaacc tttcggtata cattataaaa aagctccgat
    13081 caaagttaaa ctatttgagc gtcaactttg attggagctt ttcactttta taacacatca
    13141 atgattaagc tgatgtttta ctattattaa ttaaattgcc ttctgcgtcg tatagttctg
    13201 gttcagtttc ttcattaatt gtttgtgctg taataactac cttcgttaca ttttcgttag
    13261 aaggcacatc aaacataata tcgattaacg attcttctat gattgaacgt aaaccacgcg
    13321 cacctgtttt tctttcaatt gctttttcac taattgctga taaagcttct tcagtgaact
    13381 ctaaatccac atcatctaat tccagcattt tagtatattg tttcacaagt gcatttttag
    13441 gttgcgttaa gatgtttttc aacgcagtta catctaatgt ttctaaatta gctacaattg
    13501 gcacacgtcc gataaattca ggaatcaaac cataggcttg caaatcttct gggcgaattt
    13561 gtgctaataa tgcttgttcg tcatatttat cagcttcatt gcttgagaaa ccaataactt
    13621 tttcaccaag acggcgctta atcacttctt caataccatc aaaggcacca ccaagaataa
    13681 ataagatatt tgttgtatca atttgaatca tttcttggtt tggatgtttg cgtccacctt
    13741 gtggcggaac acttgcagtc gtaccttcta agattttaag caatgcttgt tgaacacctt
    13801 cacctgaaac gtcacgtgtt atagatgtgt tttcagattt acgtgcaatt ttatcaattt
    13861 catctacata aataatacct ttttcggctt tatcaatgtc aaagtcagct gcttgaatta
    13921 atctcaacaa gatattttca acatcatcgc ctacataacc agcttcagtt aaacttgtcg
    13981 catctgcaat tgcaaatggt acattcaacg tcttggctaa ggtttgagct aataatgttt
    14041 taccactacc tgttggccca attaatgcaa tgttactttt ttgtaattca acatcatctt
    14101 cttttggtcc taattgttga atacgcttat agtggttata aacagctaca gctaaagatt
    14161 ttttagcttt ttcttgacca ataacatatt cgtttaaatg atccataatt tctttaggag
    14221 taggtaattc tgtcatcgct tcagaagtgt tttgagctaa ttcttcttcg acgatttctg
    14281 agcataattc aatacactca ttacaaatat atacaccact tcctgctaca agttttttta
    14341 cttgatcttg gtcttttccg cagaaagagc atttcaaatt ttcttcatct tcattgaatt
    14401 taaacattct ttttacaccc ctattcgtta aagactatac tagattggat gttacaatgc
    14461 aacatattaa catacaaact ttttgcttaa agaatagtag cagatacata agctatgcat
    14521 ctgctactca tttttgtaat ttaatattta atgaagatta atcttcttta gttccttcaa
    14581 cgaactttgc gttatctctt aataaatcga taactttttg gatacgaaca tcatttttaa
    14641 tgatatcagt attacctaaa gtatttttga tatcttcaac tgagatatta aattgtttac
    14701 tcattttttc taattcttta tcgatatctt catcagtagc ttcgattttt tcagcttcag
    14761 cgatcgcagt taaagttaag ttagttttaa cacgttgttc tgcatcgtct ttcatttgct
    14821 ctcttaattg agtttcatct tgacctgaga tttggaagta cgtttgtaaa tctaaacctt
    14881 gttgttgaat tctttgtgca aattcagaca ccatacgatc taattcagta ttaaccattg
    14941 cttcaggaat atcgattgtt gtattatcag tagcttttgt aatcgcttct tctttttcaa
    15001 cattttcagc atctgtagct ttttgttcag ctaaacgttt acgtaagttt tctttgtact
    15061 cgtctactgt atttgcttct gcatctaatt cattagcaat ttcatctgtt aattctggga
    15121 cttctttaaa tttaatttcg ttaacttttg ttttgaaagt tgcttcttta ccggctaatt
    15181 cttcagcatg gtattcttct gggaatgtta cgacaacatc tttttcttcg tcaactttca
    15241 taccttctaa ttgctcttcg aaaccaggta tgaatgaacc tgaaccgatt tctaaatcgt
    15301 aaccttcagc ttgtccacct tcgaattctt ctccgtcaac tgaaccacta aagtcgatgt
    15361 taactgtgtc gccattttca acaacaccat cttctttaac gaccatttca gctaaatgtc
    15421 ctaagctgtg gtcaatcgct tcttgtaact catcatcaga taattcagtt tcttgttttt
    15481 caatttcaag acctttatag tctcctaatt taacttctgg ctcaactgta actgttgctt
    15541 caaaaatgaa atctttacct ttttcaattt gagtaacact tacttctggt tgtgcaactg
    15601 gtttaatatc agtttcgtca attgcttcac cataagcatc tggtaataaa atgtcgatag
    15661 catcttgata taatgcttct acaccaaagc gttgttcaaa aattggacgt ggcactttac
    15721 ctttacggaa tccaggtacg ttaatttgtt taaccacttt tttgaatgct tgatctaacg
    15781 ctttgtttac tttttctgca ggaacagtaa cagttaataa accttcgtta ccttcctttt
    15841 tttcccaagt tgctgtcatg tatatatacc tccatgatta actaatttat tttttcaact
    15901 tccctattat atcataggtc tattccctat acaaacattg aaatcacaac gtttatatat
    15961 ttgtaaatca acttttttcg tcaaaactat attaaaatgc tgagtattat tttgcaacaa
    16021 tttaactatt attattcaaa tctaattgct gaataaaatt aaatgcatcg atactttgta
    16081 attcacattt aagtcctaac atatttttga aataacattc gtatgcatta atccatttat
    16141 tcgtttcaaa caaagtttca atatcaattg gataaatcat gatagagtga ttattcataa
    16201 tatggtgtgc ttcttcagcg atatgtaaag caccatcatt taatgtttcc ataacgttag
    16261 gtataacttt ttctttaagt gttgtatgtt ctagcccttt taaattagct ggtacaaaag
    16321 ttacatccat accatacttt tcaattgtca gttcttgtgt acaatttgcg aaccttaaat
    16381 actcaatcat taaactaatg aggttataac tgtacgtatt agattttaat atatataata
    16441 ccgtttcttg aaattgaaaa tgaccattgt ctattaactt caaaatcaag tgcgtctgtt
    16501 ccctcattga taacgtatca aaatcagcta atgactgagt caatcgtttt tcatcttcaa
    16561 ttaattttga cttagcaaat tctttaagag gatgtaatgc cattcttgtt ttgtgatctt
    16621 tgacttcatc aataatttga tgaattactt ctaccgcttc aaaatattgt cccaaaccaa
    16681 tcaaactttt cacgtaataa atcatcaatg catcatattg ttgtatgcca gttttcaata
    16741 agacaattgt ttcttctctt aattcaagaa atgaccccgt ctcatataac atacggcatt
    16801 tcatcattgc tatttcttca gtgagttcaa attgacgttc atattgcaca atatattcat
    16861 acatcaggtc atagttttgg tcttgttcag cacgttttat atctttatat aactttttcg
    16921 aagagttagg aaactgaata atatctgaca tatatgtcac ctcgtttgat tataaatctt
    16981 gaaggattaa tgcactccac gtttcgaaat catcatatga ctctatgttg cctttttcaa
    17041 cccattttat tcgtaaagta tctgtttctt tagcttctac atcattcgta cttacagtga
    17101 ttttaaatac aacaccaata tgtaccttgc ccacttcatt attatcgtca ttaataaaac
    17161 cgatatattc catattttgt gaatcttgct cacttaaacc tacttcttct tctaattctc
    17221 tctgtgcatt aactctcaat acttcgttaa tagattctgc tcctggaaca tcattcatat
    17281 gaccgcctac acctattgaa gattgtccat gcaatcgagc ttctccaccg ccagataatc
    17341 gttcatacac taatatctcg ccatgctcat tttcaagtaa acaataagaa atgagttgtt
    17401 tatatgatgg atcctcttcc atatcgccgc gtcgcttaac ttcatattga cttagcgcat
    17461 caaaaatatt ttgaccttct ggtttattct tatttaaaaa cccattgaaa gtattctttt
    17521 cattattaaa aataatttct ctaggtacta caatgatttg ttcatcaaat ttagacatcg
    17581 tataaaactc ctcactttaa tgtttgttct cttaaatgca ttttagcaaa tatgtcatat
    17641 tttagcgatt aaatgtggca ttttgctatt taaaaaataa aaaagagatg acaatgccat
    17701 ctctttttta taagtgttat tattttaaag catctttagc tttagttact aattgagcaa
    17761 atgctttttc gtcagaaatt gcgatttctg ataacatttt acggttaatg tcgataccag
    17821 cttttttcaa accgttcatt aaacgtgagt agctcatttc atgttgacga gctgctgcgt
    17881 tgatacgtgt aatccataat ttacggaagt cacgtttacg ttgacgacgg tcacggaaag
    17941 catattgacc tgatttcatt acttgttgct tagctacttt gtataatgta tgttttgaac
    18001 cgaagtaacc tttagctaat ttaatcgttt ttttacgacg cgctcttgtt actgttccac
    18061 ctttaactcg tggcataaaa aattcctcct taggtaattc ctaccgattt ctgtatttgt
    18121 tcttattttt tgtatgctaa taattgtttt acacgtttca tatcgctctt agacactaat
    18181 ctagctttac gtaattgacg tttttgttta gtgctcttgt ttgcgaataa gtgagatgtg
    18241 aaagctcttg aacgttttaa ttgacctgaa gcagttcttt taacacgttt agctgctccg
    18301 cggtgagttt tcatttttgg cataatataa gtcctcctgt aatagcttta attatttttc
    18361 agctgttggc gctaacatga taaacatttg acgcccgtcc attttaggtt tttgttcaac
    18421 tgttgctata tctttgcatt catctgcata tttttctagc acacgttgac caatttcctt
    18481 atgcgtaatg gcacgccctc tgaaacgaat agatacttta catttatcgc ctttagttaa
    18541 gaatttacgt ccgtttttca acttcgtttg gaaatcatgt tcctcaattg ttggacttaa
    18601 acgaatttct ttaacattga taattttttg tttctttttc atttcttttt cttttttctg
    18661 ttgttcgaat ttgaatttac cgtaatccat aattcttgca actggtggtt tcgcattcgg
    18721 tgcaacgacc actaagtcta aatctacacg ttcagccatt tctaaagctt cacgctttga
    18781 tttaacacca atttgttcac catcttgacc gattaaacgt aattcttttg cacgaatttt
    18841 gtcattgatt tgagtttgat cttttgctat ggttgacacc tccaaaattt ttacgaaatt
    18901 tgcaccaagc aaaaaggaag agcaggtata aaatacccgc tcttccttat acacagttat
    18961 gtgtaatgtg attaacctgc caactgcttt atgcgtcgct acaggtgaga agcgggtgct
    19021 tctacttggt tcgtttcgta ttcaacgtta ttaatcatat caacaattca catttaagtc
    19081 aacactataa ctgtaattat ttttatttta accttttatt tcatccattg acacgtcttg
    19141 acgtaaatct acttgttcta atggaatttt tttcgtttta tatcgaagct tatgataaat
    19201 aaagaatgct aaaaatactg gaattcccat atacgtaatt aagaagcgac taaaattaaa
    19261 atctcctgtt ttaataaagt caacatcttg accaataatt actacaatac ataaaaagcc
    19321 agcaaataat ggtccgaatg ggaataattt agcagtatat tttaatttag atttgtcata
    19381 attttgttta tcaaatgctc ttctaaatcg ataatgactt actgctatac ctacccaagc
    19441 aataaaacca gttaaaccac ttgctgcaac gatatatttg tatgcacctt ttgtaaggct
    19501 ttgtaatacg aaaattatca ctacaatgat tgctgtaacg agtaacgaca tatatggcac
    19561 accgttttta tttgttttac caaatgcttc aaacgctaat ttatctttac tcattgaata
    19621 tagcattcga gttgaagcat acatacctga gttacctgct gataacacag acgttaaaat
    19681 gactgcattc ataaatgatg ctgcaaacgc aaatccagca tttttaaaca ctaatgtgaa
    19741 tggagacgtt gctacattat cactaccccc cattaatgca ctactatcat aaggaattaa
    19801 cataccgata acaaaaatgg ctaaaatgta aaataataaa attctccaga atacttgttt
    19861 aattgctttc ggcacagcac gttcaggatt ttctgattca ccagccgtaa taccaattaa
    19921 ctcagtacct tggaatgaga aaccagcgat taagaataca cctagaattg ataacaaact
    19981 tcctcctaag ttgccaccaa gaattggacc ttcaccttta ttaaatattt cgaatcctac
    20041 aacatgacca cccatgattc cgacaatcgt taataaacca attgcaatga aaacaataac
    20101 tgtaaccact tttatcaatg ccaaccagta ttcactttca ccatagacgc gaactgataa
    20161 cgaattcaga ctaaaaatta taactaagaa caacgcactc catgcccaag cgggtatgcc
    20221 ttgcaatggt gtccaatatt gaatgacttg tgctgcaatc gtaatatctg ctgctacagt
    20281 cactacccag ttaaaccaat agttccaacc aagcgcaaac cctaaagatg gatcaacaaa
    20341 tcttgtagca tatgtactaa atgaacctga tactggcaaa tacgtagcca tttcgccaag
    20401 tgacgtcatt aagaaaaata ccattattcc gataattgcg tatcctatta atgcacccaa
    20461 agcacctgca tcatgaattg ctccaccaga agttacaaat aaacctgtac caatacaacc
    20521 cccaatcgca atcatagaaa tatgacgatc tttaagtccc cttttgacaa cattgttact
    20581 ttcattttga acttttgaca tataagctcc ctttccatgt tttagaggcc aagaaataaa
    20641 taacctttat tcactttcat accctttttt acagaacata gtgatattaa aagtaaaaaa
    20701 gtggctacat acctttaaga tatgcaacca ctttggcaat tatctatata aggtagcaca
    20761 tcacattctg tgacagtaca gttcctattc gaaaactgtc ccaatagata taatttatgg
    20821 tttatatcta tttcggcatc tttacctttc acttgttcaa cttatgtacc ataaatactt
    20881 ctgacaagtt actaattaaa catgcaacct ctaactcaat ttaatatttt aactaacttg
    20941 taatatacag gattcatcac gcataatcaa ccctgtaaaa cttgatacgc aataaaagtt
    21001 ttaaagcatt ttattgcgac aactgtctat ctatgttttt tcaaacgaat ttcatcaact
    21061 agattccaga taaattcatc tttttcaact gtttcttggt cttgcgatcc atattgacgc
    21121 acattcactt gattattttc aacttcctta tccccaacta cgatttgata aggtattttt
    21181 tgcatttgag cttctctgat tttataaccc attttttcat tacggtcatc aatacttaca
    21241 cgaacgcctt gagatttcaa ttcatcttgt aattggcgcg cataatcata atgtaaatca
    21301 acgttaactg gaatgatttg aacttgtttt ggcgctaacc aagttgggaa tgcaccttta
    21361 gtttcttcag ttaaaaatgc tacaaatcgt tccattgttg atacaacacc acgatgaata
    21421 acaactggac gatgatgttc accatcttga ccaatataag ttaaatcaaa acgttctggt
    21481 aataagaaat caagttgtgc tgttgataat gtctcttctt tacccatcgc tgttttaact
    21541 tgaacatcta gtttcggacc atagaatgcc gcttcaccaa tcgcttcttc gtacgataag
    21601 ccaagctcat ccgctgcctc tttaagcata ttttcagctt tattccacat atcatcatca
    21661 tcaaagtact tttctttatc ttcagggtct ctataactta atctaaagct ataatcctcg
    21721 aaaccaaagt ctttatacac atcaataatc atgtttacaa cgcgtttgaa ttcttcttta
    21781 atttgatcag gtcgaacaaa gatatgtgaa tcatttaaag tcataccacg aacacgttgt
    21841 aatcctgata cagcaccact tgcttcatat ctatgcatcg ttcctagctc agcgatacgg
    21901 ataggtaatt cacgatatga atgtggttta ttcgcataaa tcatcatatg atgtggacag
    21961 ttcattggac gtaataccat agattcagtt tcatctaact gcattggtgg gaacatatct
    22021 tcttgatagt gatcccagtg accagatgtt ttgtataaat caacattagc aagtactggt
    22081 gtataaacgt ggtcatatcc catgctaact tctttatcaa caatgtaacg ttcaatttca
    22141 cgtctaattg ttgcaccgtt aggtaaccat aatggcaaac cagcaccaac taattggcta
    22201 tttgtgaata gttctaactc tttaccaatt ttacgatgat cacgttcttt acgctcttct
    22261 aacatttgta aatgtgcttt caattctttt ttatcaaaga aagcagtacc gtatatacgt
    22321 tgtaacattt tgttgttact atctccacgc cagtatgcac ctgctgtaga taatagttta
    22381 aactctttaa ttttagctgt tgatggaacg tgaactccac gacataaatc agtaaaatca
    22441 ccttgactat ataatgttac attttcatct tcaggaatcg cgtcgattaa ttctaatttg
    22501 tattcatcat tgctgaataa ctctttcgct tcatctcgtg aaaccacttt tcgttcgatt
    22561 ttcatatttt cgttaacgat ttgtttcatt gttttttcaa tttgttcaaa gtcatcagat
    22621 gagatgtttt ggtcaatgtc gaagtcatag tagaatccac cttctattac aggacctaca
    22681 ccaaatttaa cattaccata taacctttta atcgcgtgtg ccattaaatg tgcagtagaa
    22741 tgacgtaata cctctaacgc ttcttcacta cctggtgtca caatttcaat tgatccatca
    22801 gtttcaagcg gtttagttaa atctacaagt tgcccgttaa atttgccggc aacagctttt
    22861 ttacgtaatc caggactaat tgattgtgct atatcttcag tagtagtacc tttatcaaac
    22921 gcctttttat taccatctgg aaattgaata ttaatttgtt ccatgttaaa ccctcctatt
    22981 tgctttataa atacttttat ctgacctcgt taaaaaattg aacaacaatc atcgttttaa
    23041 cattaaacct attaacaata aacagggaat ttatgacacc aacaaacatt tatgtctgaa
    23101 aaaaacaaaa aatcccgccc ctatacaagg gacgagatcg tcgtggtacc accctagtta
    23161 tctaatacaa tttatgtatt aaatatctc
//
LOCUS       DANRDI010000028        23172 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.028, whole genome shotgun sequence.
ACCESSION   DANRDI010000028 DANRDI010000000
VERSION     DANRDI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.028"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..657
                     /locus_tag="PZ377_000841"
     CDS             <1..657
                     /locus_tag="PZ377_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDM6357727.1"
                     /translation="LGKQTVEKLDYNRIEIKRGEIIQSDIEREEDDKGYFKRYALTSD
                     GVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQMEKRMRKIEQLLIESPVEAN
                     LQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQIRQLHPFPTSVIQDAVNKAK
                     KVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPFLPHEIEEKGKIIATEIKEMV
                     "
     gene            658..1524
                     /locus_tag="PZ377_000842"
     CDS             658..1524
                     /locus_tag="PZ377_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDM6357728.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            1618..1911
                     /locus_tag="PZ377_000843"
     CDS             1618..1911
                     /locus_tag="PZ377_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDM6357729.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            2045..3589
                     /gene="miaB"
                     /locus_tag="PZ377_000844"
     CDS             2045..3589
                     /gene="miaB"
                     /locus_tag="PZ377_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDM6357730.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            3590..3955
                     /locus_tag="PZ377_000845"
     CDS             3590..3955
                     /locus_tag="PZ377_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDM6357731.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            3982..4473
                     /gene="thiW"
                     /locus_tag="PZ377_000846"
     CDS             3982..4473
                     /gene="thiW"
                     /locus_tag="PZ377_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDM6357732.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            4776..7394
                     /gene="mutS"
                     /locus_tag="PZ377_000847"
     CDS             4776..7394
                     /gene="mutS"
                     /locus_tag="PZ377_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDM6357733.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            7407..9416
                     /gene="mutL"
                     /locus_tag="PZ377_000848"
     CDS             7407..9416
                     /gene="mutL"
                     /locus_tag="PZ377_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDM6357734.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            9422..9964
                     /locus_tag="PZ377_000849"
     CDS             9422..9964
                     /locus_tag="PZ377_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDM6357735.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            10429..11247
                     /locus_tag="PZ377_000850"
     CDS             10429..11247
                     /locus_tag="PZ377_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDM6357736.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            11376..12872
                     /gene="glpK"
                     /locus_tag="PZ377_000851"
     CDS             11376..12872
                     /gene="glpK"
                     /locus_tag="PZ377_000851"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDM6357737.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            13030..14703
                     /locus_tag="PZ377_000852"
     CDS             13030..14703
                     /locus_tag="PZ377_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDM6357738.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            14852..15766
                     /locus_tag="PZ377_000853"
     CDS             14852..15766
                     /locus_tag="PZ377_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6357739.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            15784..16719
                     /gene="miaA"
                     /locus_tag="PZ377_000854"
     CDS             15784..16719
                     /gene="miaA"
                     /locus_tag="PZ377_000854"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDM6357740.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            16734..16967
                     /gene="hfq"
                     /locus_tag="PZ377_000855"
     CDS             16734..16967
                     /gene="hfq"
                     /locus_tag="PZ377_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDM6357741.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(17189..17665)
                     /locus_tag="PZ377_000856"
     CDS             complement(17189..17665)
                     /locus_tag="PZ377_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDM6357742.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            17777..19015
                     /gene="hflX"
                     /locus_tag="PZ377_000857"
     CDS             17777..19015
                     /gene="hflX"
                     /locus_tag="PZ377_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDM6357743.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            19034..20272
                     /locus_tag="PZ377_000858"
     CDS             19034..20272
                     /locus_tag="PZ377_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDM6357744.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            20515..20883
                     /locus_tag="PZ377_000859"
     CDS             20515..20883
                     /locus_tag="PZ377_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDM6357745.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            20902..22242
                     /gene="glnA"
                     /locus_tag="PZ377_000860"
     CDS             20902..22242
                     /gene="glnA"
                     /locus_tag="PZ377_000860"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDM6357746.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            22735..22932
                     /locus_tag="PZ377_000861"
     CDS             22735..22932
                     /locus_tag="PZ377_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357747.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
BASE COUNT         8621 a         3140 c         4377 g         7034 t
ORIGIN      
        1 ttaggtaaac aaactgttga aaaattagat tataatcgta ttgaaattaa acgtggtgaa
       61 atcattcaat ctgatattga acgtgaagaa gatgataaag gttatttcaa gcgttatgcg
      121 ttaacatccg atggtgtttc tcctagacct atccccggtg ttaaaggagg tattcatcat
      181 ataactggtg tggaacacaa tgaagaaggt aaacctagtg aatctgcgtc aaatagacaa
      241 caacaaatgg aaaaacgaat gcgtaaaatt gagcagttac taattgaatc gccagtagaa
      301 gctaacttac aacatgagga tgcagatatt ctttatatcg gttttatttc tacaaaaggt
      361 gcaattcaag aaggtagtaa ccgtttgaat caacaaggca taaaagttaa cactatacaa
      421 attagacaat tgcatccatt cccaacaagc gttattcaag atgcagttaa taaagcgaag
      481 aaagtcgttg tagtggagca caattatcaa ggacaattgg ctagtattat aaaaatgaat
      541 gtcaatattc atgataagat tgaaaattat acaaagtatg atgggacacc tttcctacca
      601 catgaaatcg aagaaaaagg caaaataatt gctactgaaa taaaggagat ggtatagatg
      661 gcgacattta aagattttag aaataatgtt aagcctaact ggtgccccgg atgtggcgat
      721 ttctcagtac aagctgcaat tcaaaaagca gccgcaaata tagggttaga acctgaagaa
      781 gtagctatca tcaccggtat aggatgttct ggccgtcttt caggatatat taattcttat
      841 ggcgttcatt ctattcacgg acgtgcatta cctttagctc aaggtgtaaa aatggcgaat
      901 aaagatttaa ctgttattgc atcgggagga gatggtgatg gttatgctat aggtatgggg
      961 catacaatcc atgctttaag aagaaatatg aacatgacgt atatagtcat ggataatcaa
     1021 atttatggtt tgacaaaggg acaaacatcg ccgtcatcag cagtaggatt tgttactaaa
     1081 acaacgccaa aaggtaatat agaaaaaaat gttgcgcctt tagaattagc attatcatct
     1141 ggtgccacat ttgtagccca aggtttttca agcgatatta aaggattaac aaaactaatt
     1201 gaagatgcaa ttaatcatga tggattttca ttcgttaatg tcttttcacc atgtgtgact
     1261 tataataaaa ttaacacata cgattggttt aaagaacatt taacaagtgt tgatgacatt
     1321 gaaaattatg attctacaga taaacaatta gcgactaaaa ctgttattga acatgaatct
     1381 ttagtaactg gtattgttta tcaagataaa gaaacaccat catatgaatc tcaaattaaa
     1441 gagttagatg atacaccact tgctaaaaga gatatcaaaa ttactgaaga caccttcaat
     1501 gcattaactg aacaatttat ttaataatta aaactgagtc gtataaatgt atttataaca
     1561 gatccattta tgctactcag ttttttacta ttacaaaaaa taaaggagtt tttaaaaatg
     1621 aaagacacat taatgagtat acaaataatt cctaaaacac caaacaatga caatgttata
     1681 ccttacgtag acgaggcgat taaaataatt gacgaatctg gtttgcattt tagagtaggt
     1741 ccgttagaaa cgacagtaca aggaaatatg aatgaatgtt taattttaat acaatcatta
     1801 aatgaacgaa tggtggaact tgaatgtcca agtattatta gccaagttaa gttttatcat
     1861 gtgccagatg gcatcactat tgaaacttta actgaaaaat atgatgaata acattaaaag
     1921 tgaagtaaac tggatttgaa ttggcttgtt agagatgacg tataacttta actgtttttg
     1981 cactttatag ttaaatttaa tataattatt aaatgatacg ggcaaataga aaggattttg
     2041 taaagtgaac gaagaacaaa gaaaagcaag ttctgtagat gttttagctg agagagataa
     2101 gaaagcagaa aaagattata gtaaatattt tgaacatgtt tatcagccgc ctaatttaaa
     2161 agaagcgaaa aaaagaggta aacaagaagt tcgttataat agagatttcc aaattgatga
     2221 aaaatatcgc ggtatgggga acgagcgtac atttttaatt aaaacatatg gatgtcaaat
     2281 gaatgcacat gacactgagg tcattgctgg tatacttgaa gcattaggct atcaagcaac
     2341 gactgatatt aacactgcag atgttatttt aattaataca tgtgcgatta gagaaaatgc
     2401 cgagaacaaa gtgtttagtg aaataggtaa tttgaagcat ttgaaaaaag aacgacctga
     2461 tattttaatc ggtgtttgtg gttgtatgtc acaagaagag tcagtagtga ataaaatttt
     2521 aaaatcgtat caaaatgtag atatgatatt tggtacacat aatattcatc atttaccaga
     2581 aattttagaa gaagcatact tatctaaagc aatggttgtt gaagtatggt ctaaagaagg
     2641 agacgttatt gaaaatcttc caaaagtccg tgaaggcaac attaaagcat gggtcaatat
     2701 tatgtatggt tgtgataagt tttgtacata ttgtattgtt ccatttacaa gaggtaaaga
     2761 acgaagccgt agacctgaag acattataga tgaagtacgt gaacttgctc gtgaaggtta
     2821 caaagaaata acgcttttag gtcaaaatgt aaattcttat ggtaaagatt tacaggatat
     2881 agaatatgac ttaggagatc ttttacaagc aatttctaaa atagcgattc caagagttcg
     2941 tttcacaaca agtcatcctt gggactttac agatcacatg attgatgtta tttcagaggg
     3001 tggtaatatc gttccgcata tccacttgcc agttcaatct ggaaataatg cagtattaaa
     3061 aataatgggt agaaaatata cacgagaaag ttatttggat ttagtaaaac gaatcaaaga
     3121 tagaattcct aatgtagcat taactacaga tattattgta gggtatccaa atgaatcaga
     3181 ggaacaattt gaagaaactt taactctgta tgatgaagtt ggttttgaac atgcatatac
     3241 gtacttgtat tcacaacgtg atggtacgcc tgctgctaaa atgaaagata atgtaccttt
     3301 aaatgtcaaa aaggaacgat tgcaacgttt gaataaaaaa gttggtcatt attcacaaat
     3361 agctatgagt aagtacgaag gacaaactgt aacagtactt tgtgaaggaa gtagtaaaaa
     3421 agatgatcag gttcttgctg gctacactga taaaaataag ctagttaatt tcaaagcgcc
     3481 taaagaaatg attggtaaac tagtggaagt acgaatagat gaagctaaac agtattcatt
     3541 aaatggcagt tttataaagg aagtagagcc ggaaatggtg attcaataaa tgtataataa
     3601 agatgacgtg ttgaaccaag cggataatat tgcaaataaa attaaaaatt tggatactat
     3661 caaaacatat caacaaattg aagcacagat tcatcagaac caaacgataa agactaaaat
     3721 ggatatgtta aaaaagcatc aaaaacaagc agtaaacttt caaaattacg ggaaacaaaa
     3781 tgcgctagaa cagtcggaac ataccattca gagtatagaa gcagaaataa atacattgcc
     3841 catagttgaa cagtttcaaa cttcacaata tgaagcgaat caattattga aaatgtttgt
     3901 atcaacaatg gaaacacgtt taaatgacca taataaagcc aagcatagtg attaattaca
     3961 aacaaaaagg agaacaatca tatgaaatca agaaaactgg ctataactgc acttttaatt
     4021 gcaataaatg ttgtattaag cagtattatc atcattcctc taggaccagt taaggcagca
     4081 ccagtacagc attttgtaaa tgtattaagt gcggtcatag taggtccttg gtatggatta
     4141 gctcaagcgc ttatatcatc aattttaaga gttctttttg gtactggtac agcttttgca
     4201 tttccgggta gtatgattgg agttttattg gctagtatgt tttacatata tcgtaaacat
     4261 atatttatgg ccgcggtcgg tgaagtactt ggaactggtg tcatcggaag tttaatttgt
     4321 ataccattag catatttcct tgggcttcaa gacttcttca ttaaaccgtt aatgattacg
     4381 ttcatagtct caagtgctat cggatctatt ataagttatt tcttattaat tactctaaaa
     4441 aaacgtggta ttcttcaaag gtttataaaa taattttatg atatatgatt tcaaacagag
     4501 tcattgttca ttttaatatt gaacgccctt acttaattaa atgtcttgaa tgttagaatt
     4561 ttaatttaat tctaaacatg ctattgacac attttattag gtttgaggcg ttttcttata
     4621 catttaagat gcttcgtgat tatatggtat gaataggttt gtaaaagtaa gtatgttaaa
     4681 atgtataagt taaataatta ataattaaaa agcttacaaa cgaatatatt gtaaagacaa
     4741 attttaaaaa gagacaaggg ttgtgaagaa ataatatgtc taatgttaca ccaatgatgc
     4801 agcaatattt aaaaataaaa tcagaatacc aagattgctt attatttttt agactaggtg
     4861 atttctatga aatgttttat gaagatgcca aggaggcatc acgtgtactt gaaattactt
     4921 taactaaaag agatgctaaa aaagaaaatc caattccgat gtgtggtgtt ccgtatcatt
     4981 ctgcagatag ttatatagat acacttgtta ataatggata taaagtagct atttgtgaac
     5041 agatggaaga tccgaaacaa acgaaaggta tggttagacg tgaggtagta agaattgtga
     5101 ctccaggaac tgtgatggag caaggtggtg tagatgataa acaaaataac tatattttaa
     5161 gttttgttat gaatcaacct gaaattgcgc ttagttactg tgatgtttct actggcgaat
     5221 taaaggttac acattttaat gatgaagcga ctttattaaa tgaaattacg acgataaacc
     5281 ctaacgaagt tgttatcaat gacaatattt ccgataattt aaaaagacaa attaatatgg
     5341 tgacagaaac aataacagtc agggaaacgt tatcatcaga aatctatagt gtgaatcaaa
     5401 ctgaacataa attaatgtat caagcgacac aattattgct agattatatt catcatacac
     5461 aaaaacgtga tttatcgcat atcgaggatg ttgttcaata tgcagctata gattatatga
     5521 aaatggattt ttatgctaag agaaaccttg agttaacgga aagcattcga ttaaaatcaa
     5581 aaaaaggaac gctactttgg ctaatggacg aaacgaaaac accaatggga gcacgccgct
     5641 taaaacaatg gatagataga ccactaataa gtaaagaaca aattgaagca cgattagata
     5701 tcgttgatga atttagtgct catttcatag aaagagacac cttaagaaca tatcttaatc
     5761 aagtgtatga tattgaacgt cttgttgggc gtgttagtta cggaaatgtt aatgcgagag
     5821 atttaattca acttaaacat tccatttctg aaataccgaa tattaaagca ttactaaatt
     5881 ctatgaatca gaatactctt gtacaagtta atcaactaga accccttgat gatttacttg
     5941 atatattaga acagagttta gtagaagaac caccaatttc agttaaagat ggcggactat
     6001 tcaaagttgg ttttaatacg caattagatg aatatcttga agcttcaaaa aacggaaaaa
     6061 catggttagc agaattacaa gccaaagaaa gacaacgtac aggaataaaa tcattgaaaa
     6121 taagctttaa taaagtgttt ggttatttta tagaaataac acgtgccaac ttgcaaaatt
     6181 ttgaaccaag tgaatttggt tatatgagga agcaaacgtt atcgaatgct gaacgtttta
     6241 taactgatga acttaaagaa aaagaagata tcattttagg tgcggaagac aaagccatcg
     6301 aattagaata tcaattattt gttcagctac gtgaagaagt taaaaaatat actgaacgtt
     6361 tacaacaaca agctaaaatt atttcagagc tagattgttt acagagcttt gcagaaattg
     6421 ctcaaaaata taattacact aggccttcat ttagtgaaaa taaaacatta gaattagtgg
     6481 aatctaggca cccagtagtg gaaagagtaa tggattataa tgactatgtg cctaataatt
     6541 gtcgattaga taatgaaaca tttatatatt taattacagg tccgaatatg tctggtaaat
     6601 cgacatatat gagacaagtt gccataatta gtataatggc ccaaatggga gcttatgtcc
     6661 cttgtaaaga ggcagtgtta cctatatttg atcaaatatt cactagaata ggtgcggcag
     6721 atgatttggt ttcaggtaag agtacgttta tggtagaaat gctagaagca caaaaggcat
     6781 taacttatgc aacagaggat agtttgatta ttttcgatga aattggacgt ggtacttcaa
     6841 cgtatgacgg tttagcttta gcgcaggcaa tgatagagta tgtagctgaa acatcacatg
     6901 ctaaaacgtt attttcaaca cattatcatg aattgacaac attagatcaa gcattaccaa
     6961 gtctaaaaaa tgttcacgtc gctgctaatg aatataaagg tgaacttata ttcttgcata
     7021 aagtcaaaga tggtgcagtt gacgatagtt atggtattca agttgcgaaa ttagctgatt
     7081 tacctgaaaa agttattagc agagcacaag tgattctaag cgagtttgaa gcgtctgctg
     7141 gtaaaaaatc atcgatatca aatttaaaaa tggtcgaaaa tgaacctgaa attaatcaag
     7201 aaaatttaaa cttaagtgtt gaagaaacaa ctgatacttt atctcaaaaa gactttgaac
     7261 aagcatcatt tgatttgttt gaaaatgatc aagaaagcga gattgaacta caaattaaaa
     7321 atttgaattt atctaatatg acaccaattg aggcattggt gaagttaagt gaattacaaa
     7381 atcaattaaa atagaggtgt tgcaaaatgg ggaaaattaa agaactccaa acctcattag
     7441 caaataaaat cgcagcaggt gaagtagttg aaagaccgag ttctgttgtg aaagaactgt
     7501 tggaaaatgc tatagatgca ggcgctacag aaataagcat tgaagtagag gaatctggcg
     7561 tccaatctat tcgcgtagtc gataatggaa gcggaattga agcggaagac ttaggattag
     7621 tatttcatag acatgcgact agtaaattag atcaagatga agatttattt catattagga
     7681 cattaggatt ccgtggtgaa gcactagcca gtatttcatc agttgctaaa gtaacattga
     7741 agacttgcac ggataatgct aatggaaatg aaatatatgt agaaaatggt gaaatattaa
     7801 atcataagcc tgcaaaagcg aaaaaaggaa cagatatact tgtagaatca ttattttata
     7861 atacaccagc acgtttaaaa tatattaaaa gtttatacac tgaactaggt aaaataacag
     7921 atattgtcaa cagaatggca atgagccatc cggacattcg aatagcactc atttcagatg
     7981 gcaaaacaat gttaagtaca aatggttcag gacgaactaa tgaagtgatg gcagagattt
     8041 atgggatgaa agttgcacga gatttagtac atatatctgg agatacaagt gattatcaca
     8101 ttgaaggttt tgttgcaaag cctgaacatt ctagaagtaa taagcactat atttctattt
     8161 ttattaatgg acgatacatt aaaaacttta tgctaaataa agcgatttta gaaggctatc
     8221 atacactctt aacaataggt aggttcccga tttgttatat taatattgaa atggatccaa
     8281 tcttagtaga cgtaaatgtt catccaacaa aactagaagt gcgtttatca aaagaagagc
     8341 aactatacca attgattgtg agcaaaatac aagaagcatt taaagaccgt atattaattc
     8401 ctaaaaataa cttggattat gtgccaaaaa aaaataaagt attacattca ttcgaacaac
     8461 aaaaaatcga atttgaacaa agacaaaaca cagagaataa ccaagagaag acgttttcat
     8521 ctgaagaaag taacagtaag ccatttatgg aagaaaatca aaacgatgag atagttataa
     8581 aagaagattc atataatcca ttcgtaacga aaacatctga aagtttaata gctgatgatg
     8641 aatcttctgg ttataataat acacgtgaaa aagatgaaga ctacttcaaa aaacaacaag
     8701 aaattttaca agaaatggat caaacatttg attcaaatga tggtacaact gtgcaaaatt
     8761 atgagaataa agcgtctgat gattattatg atgtaaacga tattaaagga acaaaaagta
     8821 aagaccctaa acgaagaatt ccatatatgg aaattgttgg acaagtacat ggaacgtata
     8881 ttattgctca aaatgaattt ggcatgtaca tgattgacca gcatgcagct caagaaagaa
     8941 taaaatatga atattttcga gataaaatag gtgaggttac caatgaagta caagatttat
     9001 taatcccgtt aacatttcat ttttcaaaag atgaacaatt agtcattgat caatataaaa
     9061 atgagcttca acaagtaggt atcatgttag aacattttgg tggtcatgat tatattgtaa
     9121 gtagctatcc agtttggttc cctaaagatg aagtagaaga aattattaaa gatatgattg
     9181 agctaatttt ggaagagaaa aaagtagata tcaaaaaatt acgtgaagat gtagcaatca
     9241 tgatgtcatg taaaaaatcc attaaagcga atcattattt acaaaaacat gaaatgtctg
     9301 atttaattga tcaattaaga gaagcggaag atccatttac atgtccacat ggtcgtccaa
     9361 taatcattaa tttttcaaaa tacgaattag aaaaattatt taagcgtgtg atgtagagag
     9421 gatgaatcaa gtgaataaca acatattgcc tgctataaga aacattaaag atttagagaa
     9481 actgattaaa acagactata aaatgtgtgt gcttctagat atgcatatag gacatataaa
     9541 aagtattatg gaattgctga agcaaaatca tatagagtgt tttattcata tagatttgat
     9601 aaaaggttta agccacgatg aatttgcaag tgaatttatt attcagcaat acaagccaaa
     9661 aggtatcgta tcgactaaat ctaaagtaat aaaaaaagct aaatcattaa atactttaac
     9721 gatttttaga gtatttatta ttgatagtca agcattgaaa cgcagtatag atttgataaa
     9781 aaaagttgaa cctgattttg ttgaagtact tccaggtgtt gcgagtaaag cgattcatca
     9841 tattcagaaa gaaacaaaca cacaagtcat tgcaggtggc ctaattaata caatagatga
     9901 agtcaatgaa gctgttaaaa atggagcgaa atatgtaact actagttatg ataaactttg
     9961 gtaaatcata agtctgcaaa aatttcattt ttcacgagat ttagtcatat aacataataa
    10021 aaaagccgtt ttcatatggg atatgcagta ggtatcaaac aaaaaataat caaataaaat
    10081 aattagcacc tctaaaacga ttaaaatcat taaagtttta gaggtgttta ttagtaaaaa
    10141 atgcaactag tcataaaaaa tatggctgaa gaatgtcaat taaacaaaag agcgtcataa
    10201 ataaattagt taggattggt atttctagtt ttcaaattaa actactttta taaagacttg
    10261 aaaaattaaa tttgacaaaa tttttacaaa aaccattgac aacgctttca tatcgatagt
    10321 atcataacaa atataataaa agttaataca tagaatagag acgggagatt tctacgagcc
    10381 aaactgctag tgtaggaatc tctttgtctt tttgggagga catttaatat gaatgtatat
    10441 ttagcagaat tcctaggaac tgcaatctta atcctttttg gtggtggcgt ttgtgccaat
    10501 gtcaatttaa agagaagtgc tgcgaatggt gctgattgga ttgtcatcac agctggatgg
    10561 ggattagcgg ttacaatggg tgtgtttgct gtcggtcaat tctcaggtgc acatttaaac
    10621 ccagcggtgt ctttagctct tgcattagac ggaagttttg attggtcatt agttcctggt
    10681 tatattgttg ctcaaatgtt aggtgcaatt gtcggagcaa caattgtatg gttaatgtac
    10741 ttgccacatt ggaaagcgac agaagaagct ggcgcgaaat taggtgtttt ctctacagca
    10801 ccggctatta agaattactt tgccaacttt ttaagtgaga ttatcggaac aatggcatta
    10861 actttaggta ttttatttat cggtgtaaac aaaattgccg atggtttaaa tcctttaatt
    10921 gtcggagcat taattgttgc aatcggatta agtttaggcg gtgctactgg ttatgcaatc
    10981 aacccagcac gtgatttagg tccgagaatt gcacatgcga ttttaccaat agctggtaaa
    11041 ggtggttcaa attggtcata tgcaatcgtt cctatcttag gaccaattgc cggtggttta
    11101 ttaggtgcag tggtatacgc tgtattttat aaacatacat ttaatattgg ttgtgcaatt
    11161 gcaattgttg tagttattat tactttgatt ttaggttaca ttttaaataa atcatcaaaa
    11221 aaaggtgata tcgaatcaat ttactaaaat aaaaagaaac gtaaatagca taatttaaca
    11281 tgtttgattc atggattatg ctattttttc gccaaaattt aacagatttt gtacaatggg
    11341 ttagcgatta ttttttaata aaggagatac tactaatgga aaaatatatt ttatctatag
    11401 accaaggaac aacaagctca agagcgattt tattcaatca aaaaggggaa attgcagggg
    11461 tagcacaacg tgagtttaag caatattttc cacaatcagg ttgggttgaa catgatgcaa
    11521 atgaaatttg gacatctgtg ttagctgtaa tgacggaagt aattaatgaa aatgatgtta
    11581 gagctgatca aattgcaggt atcggtatta caaaccaacg tgaaacaacg gttgtttggg
    11641 acaaacatac tggccgccca atttatcacg caattgtttg gcaatcacgt caaacacaat
    11701 caatttgttc agaattaaaa caacaaggat atgaacaaac atttagagat aagacaggat
    11761 tacttttaga tccgtatttt gcaggtacaa aagttaaatg gattctagac aatgttgaag
    11821 gtgcacgaga aaaagcagaa aatggcgatc tattatttgg aacgattgat acttggttag
    11881 tatggaaatt atcaggaaaa gctgcgcata ttactgatta ttcaaatgcg agtcgtacat
    11941 taatgtttaa tatccatgat ttagaatggg acgatgagtt attagaacta cttacagtac
    12001 ctaaaaatat gttgccagaa gttaaagctt cgagtgaagt atatggtaag acaattgatt
    12061 accacttcta tggtcaagaa gtaccaatcg ctggagtagc tggtgatcaa caagcagcat
    12121 tatttggaca agcttgcttc gaacgtggtg acgtgaaaaa cacatatgga actggtggct
    12181 tcatgttaat gaatacaggt gacaaagcgg ttaaatctga aagtggttta ttaacaacaa
    12241 ttgcttatgg tattgatgga aaagtaaatt atgcgcttga aggttccatc tttgtttcgg
    12301 gttcagcaat ccaatggtta cgtgatggat taagaatgat taattcagca ccacaatcag
    12361 aaagttatgc gacacgagtt gactctactg agggtgttta tgttgttcca gcttttgtag
    12421 gtttaggaac accatattgg gattctgaag cacgtggtgc gattttcggt ttaacacgtg
    12481 gaactgaaaa agagcacttt atccgtgcaa ctttagaatc actatgttac caaactcgtg
    12541 acgttatgga agcaatgtca aaagactctg gtattgatgt ccaaagttta cgtgtcgatg
    12601 gtggtgcagt taaaaataac tttattatgc agttccaagc agacattgtt aatacttctg
    12661 ttgaaagacc tgaaattcaa gaaactacag ctttaggtgc tgcattttta gcaggtttag
    12721 cagttggatt ctgggagagt aaagatgata tcgctaaaaa ctggaaatta gaagaaaaat
    12781 tcgatccgaa aatggatgaa ggcgaaagag aaaaattata tagaggttgg aaaaaagctg
    12841 ttgaagcaac acaagttttt aaaacagaat aaacttgtag attagacttt tgtataaaca
    12901 ttgtgataca atcaatttaa gttaatattt gaatcgagaa gcgagagatt tgttcgaaca
    12961 tgtacaattg aaggattgtt tatgtgggac aagtctctcg tttttacatt ttttaggagg
    13021 cgttttggaa tggcattgtc tacttttaag agagaacata ttaaaaagaa tttaagaaat
    13081 gatgaatatg atttagtaat tattggtggc ggtattacag gtgcaggtat tgcactagac
    13141 gcgagtgaaa gaggaatgaa agttgcatta gttgaaatgc aagactttgc acaaggaaca
    13201 agctcaagat ctacaaaatt agtccatggt ggtttgcgtt acttaaaaca attccaaatt
    13261 ggagtagttg ccgaaactgg taaagaacgt gcgattgttt atgaaaatgg gcctcatgtt
    13321 acgactccag agtggatgct tttaccaatg cataaaggtg gaacatttgg taaattctca
    13381 acatcaattg gtttaggaat gtatgatcgt ttagcaggtg ttaagaagtc tgaacgtaaa
    13441 aaaatgttat ctaaaaaaga aactttagct aaagaaccat tagttaaaaa agaaggtcta
    13501 aaaggcggcg gttactatgt tgaatatcgt actgacgatg cgcgtttaac tattgaagtt
    13561 atgaagcgtg ctgctgaaaa aggcgcagaa attatcaact atactaaatc tgaacacttc
    13621 acttatgata aaaatcaaca agtaaatggt gttaaagtta tagataaatt aactaatgaa
    13681 aattatacaa ttaaggctaa aaaagtggtt aatgcagcag gtccatgggt tgatgatgtt
    13741 agaagtggtg attatgcacg caataataaa aaattacgtt taactaaagg tgtacatgtt
    13801 gttattgatc aatcaaaatt cccattaggt caagcagtat actttgatac tgaaaaagat
    13861 ggaagaatga tttttgcaat tccacgtgaa ggaaaagcgt atgtaggtac tacagataca
    13921 ttctatgaca atatcaaatc ttcaccatta actacacaag aagacagaga ctatttaatc
    13981 gatgcgatta attacatgtt ccctagtgtt aatgttacag atgaagatat tgaatcaaca
    14041 tgggcaggaa ttagaccatt aatttacgaa gaaggcaaag acccttctga aatctctcgt
    14101 aaggatgaaa tttgggaagg taaatcaggt ttattaacta ttgcaggtgg taaattaaca
    14161 ggctatcgtc acatggctca agacattgtt gatttagtat ctaaacgctt gaaaaaagac
    14221 tacggtttaa catttagtcc atgtaataca aaaggtctgg caatttcagg tggcgatgta
    14281 ggtggtagca agaactttga tgcgtttgta gagcaaaaag tagatgtagc taaaggattc
    14341 ggcattgatg aagatgttgc aagacgttta gcatctaaat atggttcaaa tgttgatgaa
    14401 ttgttcaaca ttgcgcaaac atctcaatac catgatagca agttaccatt agaaatttat
    14461 gtagaacttg tttatagtat tcaacaagaa atggtataca aacctaacga tttcttagtt
    14521 cgtcgttctg gtaaaatgta tttcaatatt aaagatgtat tagattataa agatgctgtc
    14581 atcgatatta tggcagatat gcttgattac tctccagctc aaattgaagc atatactgaa
    14641 gaagttgagc aagcaattaa agaagcgcaa catggaaata atcaaccagc agttaaagaa
    14701 taattaattt gtacaatcat aaactggtgt cctgttttaa gggcatcagt tttttatacg
    14761 agatacatta gtcaattgaa actatgcatc actaaatgta tgataataat aaaagcgaat
    14821 ataaaagcgt tgttattaaa ggtgtgagat gatgactgaa aaacaattta aattaactgt
    14881 acaagataat acgaatattg aagttaaagt gaattttaca gatgtagatt caaaaggaat
    14941 tattcatata tttcatggta tggctgaaca tatggaacgt tacgataaat tagcacatgc
    15001 actttcaaag catggcttcg atgtgatacg tcataatcat cgaggacatg gtattaatat
    15061 tgatgaatca acaagagggc attacgatga tatgaaacga gttatcggtg atgcctttga
    15121 agtagcgcaa acagtgagag gcaatgttga taaaccatac attataatcg gacattcaat
    15181 gggatccgtt atagctagat tgtttgtaga aacatatccg caatatgttg atggtctaat
    15241 tttaagtggt actggtatgt attcattatg gaaaggttta ccaaccgtta aagtgttaca
    15301 actgattaca aaaatttatg gtgctgagaa acgagttgaa tgggttaacc agttagtatc
    15361 aaatagtttt aataaaaaaa tacgtccatt acgtacacaa agtgattgga tttctagtaa
    15421 tccaattgaa gtagataact ttattaaaga tccatatagt ggatttaatg tgtcaaatca
    15481 attattatat caaacagcct attatatgct acatacatca caattaaaaa atatgaaaat
    15541 gttaaatcat gccatgccta tattattagt ttcaggatat gacgatcctt taggtgatta
    15601 tggtaaaggg attttaaaat tggcgaatat atatagaaaa gctggcatta aaaatgttaa
    15661 agtgaatctt tatcatcata aacgtcatga agtgttattt gaaaaagatc atgacaaaat
    15721 ttgggaagac ttgtttaaat ggttgaatca attttataaa aaataaagaa agtggaatta
    15781 aatatgaata aaaataagcc ttttattgta gtaattgtgg ggccaactgc ttcaggtaaa
    15841 acagagctta gcatcgaact cgcgaagcgt atcaatggtg aaatcataag cggtgactct
    15901 atgcaagtct acaaacatat gaatattgga actgcaaaag taacacctga agaaatggat
    15961 ggtattccac atcatttaat tgatatcttg aatcctgatg atacattttc agcatatgaa
    16021 ttcaagcgat tagcagaaga tttaattact gatataacga atagaggtaa agttccaatc
    16081 atagcaggtg gaacaggctt atatattcaa tcattaatat ataattatga attagaagat
    16141 gaaacagtta cacctgcaca attatccata gttaaacaaa agttatctgc attagaacat
    16201 ttagataatc agcaactaca cgattattta gctcaatttg atgcggtttc tgcagaaaat
    16261 attcacccta acaaccgcca aagagtgttg cgcgctattg aatattattt aaaaacaaaa
    16321 aaacttttga gtaatcgcaa gaaagtgcaa caatttactg aaaattatga tacattatta
    16381 ttagggattg aaatgtcgcg taaaacatta tattcaagaa taaataaacg tgttgatatt
    16441 atgttggatc acggattatt tagagaagtg caacaacttg ttgaacaagg ctatgaatct
    16501 tgccaaagta tgcaagctat tggatataaa gaattaatac ctgtgattaa cggacaaatg
    16561 atttatgaag atgctgtcaa tgatttaaag caacattcac gccaatatgc aaaacgacaa
    16621 atgacatggt tcaagaataa aatgagtgtt cattggttag ataaagaaaa tatgtcactt
    16681 caaatgatgt tagatgagat tacaacccag attaagtaaa aggagtccga cagatgattg
    16741 caaacgaaaa catccaagac aaagcactag agaattttaa agcaaaccaa actgaagtaa
    16801 ctgtattctt tctaaacggt ttccaaatga aaggtgttat tgaagaatac gacaagtatg
    16861 tcgtaagctt aaattctcaa ggcaaacaac acttgattta caaacatgcg atcagcactt
    16921 atacagtaga aactgaaggt caagcatcta ctgaaagtga agaataagtt gtaatcatat
    16981 gggcacgatt taatgactcg tgtacataac gtttgaagta tgataaatag atatagaaat
    17041 aaagtcatat gtgacatcaa ttaaatgatg ttcaaatgac aagatacaat agaggaatgt
    17101 ttgtaataaa aacgcttcat ataaaggtcg agtcaatata tgatacgact ttatatgaag
    17161 cgttttattt gctatgagct agtatatttt ataataattt ttctatttct ctttcgattt
    17221 gaacaggttt tttttgaggt gcaaatcgtt taacaacgtt accttcgcga tccactaaaa
    17281 acttagtgaa attccatttg attttctcat taaagaatcc gtgttgtgcc gcagtcaaat
    17341 atctaaataa aggtaattga tgttcccctt ttacgtctat tttttgatgc atagggaagg
    17401 taacaccata gtttaattta cagttttgag ctgcttcttc gcctgaacca ggttcttggc
    17461 caccaaattg attacaaggg aaacctagaa ttacaaaccc ttgatctttg tatttctcgt
    17521 ataatgattg caaaccttca aattgtgaag taaagccaca ttcgctagct gtattaacaa
    17581 ttagcataac gtcaccctta tatgcatcta atttgtaagt aacaccttta tttgtttcta
    17641 ctacaaaatc ataaattgtc tccattgtat catcctttcg atttacttaa aatgtaccac
    17701 aaaatcgtgt aatagtcttt actaaaactc tatgatagaa tactttgagt aggattttat
    17761 taaggagatg tataacatgg ctcagcaaca aattcatgat actaaaaata aactagaaaa
    17821 agctgtctta gtcggtgtac atgctcaaga tgataagcaa tttaattttg agtctacaat
    17881 ggaggaatta tcatctttat cagagacttg ccaacttgaa gtgttgggtc aaattactca
    17941 aaacagagat cgtgtagatc gcaaatatta tgttggtaaa ggtaaaattg aagaaattca
    18001 agcatttatt gagttcaaag atattgatgt agtcatcaca aatgatgaat taacgactgc
    18061 acaatccaaa tcactaaatg aagctttagg tgtaaaaatt attgatagaa ctcagttgat
    18121 tcttgaaata tttgcattaa gagcaagaag taaagaaggt aaattgcaag tagagctagc
    18181 acaacttgat tatttattac ctagattgca aggccatggt aaaagccttt ctcgtttagg
    18241 tggcggtatt ggaactagag gccctggtga aacgaagtta gagatggatc gcagacatat
    18301 tcgaactcgt atgaatgaaa ttaaacatca attgcggacg gtagaagaac atcgcgaaag
    18361 atatcgaaat aaaagaaatc aaaatcaggt gtttcaagta gctttagttg gttatacaaa
    18421 tgctggtaaa tcatcatggt ttaatgtttt agcaaatgaa gagacgtatg aaaaagatca
    18481 attatttgca acgttagatc ctaaaacacg acaaattcaa ataaatgatg gatttaattt
    18541 aattatttca gatactgttg gttttataca gaaactacct acgacgttaa ttgcagcttt
    18601 taaatcaact ttagaagagg ctaaaggtgc agatttatta gtacatgtcg tagatagtag
    18661 ccatcctgaa taccgtacgc agtatgacac agttaatgat ttaatcaaac aattagatat
    18721 gagtcatatt tctcaaatag ttatttttaa taaaaaggac ttatgtgatc atgcatcaaa
    18781 tcgtccagca agtgatttgc ctaatgtttt tgtttcttct aaaaatgatg gtgataaatt
    18841 acttgttaag acgttattta ttgatgaaat caaaaggcaa ttaacttatt atgatgagac
    18901 aattgcgacg aataatgcag atcgattata ttttctaaaa caacatacat tagtgactga
    18961 acttaaatat gatgaaattg aaaatgttta tcgtataaaa ggatttaaaa aataataaaa
    19021 ggacgaaatt caaatgaaag atataagtaa gatagtagct gacgtcgaat caacgttagc
    19081 accatatttt aaagaaattg aagaaacagc atatattaat caagaaaaag tattaaatgc
    19141 atttcatcat gtcaaagcaa ccgaaagtga tctacaagga tcaacaggat acgggtatga
    19201 tgactttgga cgtgatcatt tagaagaaat atatgcgcag gcatttaaag cagaagatgc
    19261 aattgttcgt ccgcaaatta tttcaggtac gcatgcgatt actattgcat tacaaagttt
    19321 attaaaacat ggtgatgaat taatttatat aacgggtagt ccatatgaca ctttacttga
    19381 agtcattggc gtaaacggaa atggtattga aagtttaatg gagcatggcg tatcgtataa
    19441 agatattgca cttaaagaag gtaagatcga tattgaaagt gtgttagatg gggtttctga
    19501 gcgcaccaaa gtaatagcga ttcaacgttc gaaaggctat gatcaaagac cttcaattcc
    19561 gctagatgaa attgaaaagg taattactag gttgaaaaac gtgcatccta atattttaat
    19621 atttgtggat aactgttatg gggaatttgt tgaaagacgt gaacctatag aatgtggtgc
    19681 cgatttaata gcaggatcat taattaaaaa ccctggcggt ggtttagcta agattggtgg
    19741 atacattgct ggtagaaaag atttaattga acgatgtggt tatagattga cagcacctgg
    19801 tattggtaaa gaagcgggtg catcattaaa tgcattgctt gaaatgtatc aaggtttctt
    19861 tttagcacca cacgttgtca gtcagagtct taaaggtgca ttgtttacta gtttattttt
    19921 agaaaaaatg aatatgaaca caacgccgaa gtactacgaa aaacgaactg atttaattca
    19981 aacagttaaa tttgaaacga aagaacaaat gatttcattt tgtcaaagta ttcaacacgc
    20041 atccccaatt aatgcacatt ttagtccaga acctagttat atgcctggtt acgaagatga
    20101 tgttattatg gcagctggta cgtttattca aggttcatcg attgaattat ctgcagatgg
    20161 acctattcgt cctccttatg aagcatatgt tcaaggagga ttaacatatg aacacgttaa
    20221 aattgctgtg acaagagctg ttaatcagtt gaaagaacaa ggacttatat aatagattaa
    20281 atttcaccct aaatgataat gtgttttggg tgaaattttt tgcggagatt tatcagatta
    20341 tttttaaaaa ttgaaaaaat attttagctg tagattgttt tatgtcgttt tagaagtcga
    20401 aatcgcagtt atatcaatgt tttaagcttt atgttagaaa acctgacata tttttgaaat
    20461 cctaaaaaaa ttatgataag ttattaacaa gttcaaaagt agaggagagg aacaatgata
    20521 tcgaatgatg caatcagacg aaatatggct gtcttctcta tgagtgtagt aagtaagtta
    20581 acggatttaa cgccaaggca aatacgttac tatgaaacac atgaactcat caaacctgaa
    20641 agaacagaag gtcaaaaacg tctgttctca ctcaatgatt tggaaagatt actagaaatt
    20701 aaatcattat tagaaaaagg atttaatatc aaagggatta aacaaatcat ttatgactca
    20761 caagagcatt taacaacaga tgaacaagag ataagaaaaa agatgattgt agatgccacg
    20821 caaaagccta ttggagaaac tttgccaata aatcgtggtg atttatcccg atttattaaa
    20881 taaaatttgg aggattttaa aatgccaaaa cgtactttca ctaaagacga cattcgtaaa
    20941 tttgcagaag aggaaaatgt aagatattta agattacaat tcactgatat tttaggaaca
    21001 attaaaaatg ttgaagtgcc tgtaagccaa ttagaaaaag tacttgataa cgaaatgatg
    21061 tttgacggtt cttctatcga aggtttcgta cgtatcgaag aatcagatat gtacttacat
    21121 ccagatttag atacttgggt aatcttccca tggactgctg gacaaggtaa agttgcacgt
    21181 ttaatttgtg atgtatataa aacagatgga acaccatttg aaggggatcc tcgtgcaaac
    21241 ttaaaacgtg tattaaaaga aatggaagat ttaggcttca cagactttaa cctagggcct
    21301 gaaccagaat tcttcttgtt taagttggat gaaaaagggg aaccaacttt agaacttaat
    21361 gatgatggtg gatatttcga tttagcacct acagatttag gtgaaaactg tcgtcgtgat
    21421 attgttttag aattagagga tatgggcttc gatattgaag ctagtcacca tgaagttgcc
    21481 cctggtcaac atgaaattga ctttaaatat gcagatgctg ttacagcatg tgataatatc
    21541 caaacattta aattggttgt taaaacaatc gcacgtaaac ataatttaca cgcaacattt
    21601 atgcctaaac cattattcgg tgtgaatggt agcggtatgc actttaacgt ttcattattc
    21661 aaaggtaaag aaaatgcatt ctttgatcca aatactgaaa tgggcttaac ggaaactgca
    21721 tatcaattta cagcaggtgt gcttaaaaat gcacgcggat ttactgctgt atgtaacccg
    21781 ttagtaaact catataaacg tttagtacct ggttatgaag caccatgtta tattgcatgg
    21841 agtggtaaaa accgttcacc attaatccgt gtaccatctt caagaggatt atctactcgt
    21901 atcgaagtac gttcagtaga tccagctgca aacccataca tggcgttagc tgcaatctta
    21961 gaagctggac tagatggtat taaaaataaa ttaaaagttc cagaaccagt taaccaaaat
    22021 atttacgaaa tgaaccgtga agaacgtgaa gcagtaggca ttcaagactt accttcaaca
    22081 ctttatactg cattaaaagc aatgcgtgaa aatgaagtta ttaaaaaagc tttaggaaat
    22141 catatctata atcaatttat taattcaaaa tcaattgaat gggattacta cagaactcaa
    22201 gtctctgaat gggaaagaga tcagtacatg aagcaatatt aatacgctaa acaatgatgg
    22261 gaaatcagta atatcaaggg ctagagcatt ttgctttagc tcttttttga tttggaaaga
    22321 tgggatttca cttgaccact tttgttaaaa aatggatttg gtcaagtttt ggtcaagtaa
    22381 atgttttatg tatttatttt tttaaagctg gggaagacca ttttatattt tagttcacat
    22441 gaattttaat atatttggag gtgatgagat gaggcgataa aagaacgtcg cgaaaacgtt
    22501 gagtattact tgaaaatagc catattcata ttacttctca tcgaattcat ttgcagaacg
    22561 ttctaaggta agaccccgaa aagagggacc ttactacgca ttattaaggg ggtaatacta
    22621 tatgaaaaga gaaacaaaac aaaaaatatc tttttgttta tcgatcggta ttttcatttt
    22681 agttatacta ctattgatat tctaaaaaat taaaaatttc gcctcatcta atttatgaat
    22741 tgctatgatg aaatattcaa tacaatcaaa gaattgatag aaaacaaaga gatatcgagt
    22801 tatcaaatta ataaagatac tgggataagt tacggtaata ttaatgctat gcgccgtaga
    22861 gaaagaagaa tagaaaattt aagcttaaag aatgcaaaga tcttatatga atatgcgaaa
    22921 aaggtattgt aatataagca ttgagttcaa taggcataca ataactgccc aaactagtga
    22981 ctgagtgggt gcttttatag tattaaaagt tttctaaaag tgaaagaaat catgtttagt
    23041 gatttactta tgtttttcaa aatagtataa taattttata taatatatag agcttcagta
    23101 taccttttga gtatatatgg agttctttct ttttatacaa gatgtatatg tataaatgga
    23161 aatatttaat ac
//
LOCUS       DANRDI010000029        22921 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.029, whole genome shotgun sequence.
ACCESSION   DANRDI010000029 DANRDI010000000
VERSION     DANRDI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.029"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(213..629)
                     /locus_tag="PZ377_000862"
     CDS             complement(213..629)
                     /locus_tag="PZ377_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDM6357748.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(768..2591)
                     /locus_tag="PZ377_000863"
     CDS             complement(768..2591)
                     /locus_tag="PZ377_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDM6357749.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(3069..4346)
                     /locus_tag="PZ377_000864"
     CDS             complement(3069..4346)
                     /locus_tag="PZ377_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6357750.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            4893..5504
                     /locus_tag="PZ377_000865"
     CDS             4893..5504
                     /locus_tag="PZ377_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDM6357751.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(5513..5611)
                     /locus_tag="PZ377_000866"
     CDS             complement(5513..5611)
                     /locus_tag="PZ377_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357752.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(5601..6764)
                     /locus_tag="PZ377_000867"
     CDS             complement(5601..6764)
                     /locus_tag="PZ377_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6357753.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVT
                     ITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
     gene            complement(6757..7215)
                     /locus_tag="PZ377_000868"
     CDS             complement(6757..7215)
                     /locus_tag="PZ377_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDM6357754.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            7649..8344
                     /locus_tag="PZ377_000869"
     CDS             7649..8344
                     /locus_tag="PZ377_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDM6357755.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIITETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            8346..9116
                     /locus_tag="PZ377_000870"
     CDS             8346..9116
                     /locus_tag="PZ377_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6357756.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            9489..11453
                     /locus_tag="PZ377_000871"
     CDS             9489..11453
                     /locus_tag="PZ377_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDM6357757.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            11797..12876
                     /locus_tag="PZ377_000872"
     CDS             11797..12876
                     /locus_tag="PZ377_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357758.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(12981..13574)
                     /locus_tag="PZ377_000873"
     CDS             complement(12981..13574)
                     /locus_tag="PZ377_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6357759.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(13596..14564)
                     /locus_tag="PZ377_000874"
     CDS             complement(13596..14564)
                     /locus_tag="PZ377_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDM6357760.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(14642..15076)
                     /locus_tag="PZ377_000875"
     CDS             complement(14642..15076)
                     /locus_tag="PZ377_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDM6357761.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            15303..15587
                     /locus_tag="PZ377_000876"
     CDS             15303..15587
                     /locus_tag="PZ377_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDM6357762.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            15758..16564
                     /locus_tag="PZ377_000877"
     CDS             15758..16564
                     /locus_tag="PZ377_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6357763.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(16684..17355)
                     /locus_tag="PZ377_000878"
     CDS             complement(16684..17355)
                     /locus_tag="PZ377_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDM6357764.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            17625..18617
                     /locus_tag="PZ377_000879"
     CDS             17625..18617
                     /locus_tag="PZ377_000879"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDM6357765.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(18866..19666)
                     /locus_tag="PZ377_000880"
     CDS             complement(18866..19666)
                     /locus_tag="PZ377_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDM6357766.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            19987..20622
                     /locus_tag="PZ377_000881"
     CDS             19987..20622
                     /locus_tag="PZ377_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDM6357767.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            20628..21359
                     /locus_tag="PZ377_000882"
     CDS             20628..21359
                     /locus_tag="PZ377_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDM6357768.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(21519..22139)
                     /gene="srtA"
                     /locus_tag="PZ377_000883"
     CDS             complement(21519..22139)
                     /gene="srtA"
                     /locus_tag="PZ377_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDM6357769.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            22333..22824
                     /locus_tag="PZ377_000884"
     CDS             22333..22824
                     /locus_tag="PZ377_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDM6357770.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
BASE COUNT         7552 a         4018 c         3354 g         7997 t
ORIGIN      
        1 tatataatca tctttattgt acctaacatt ttattaagat aatactaata cactatcgaa
       61 ctttgggctg atactgaaac agcacaaaga aactcaaaca attcgaatta tgtatcaaaa
      121 ccttcaatta caggaagtga ttttaaaatg atttaacgca aaaaaacacc tgttaccgtt
      181 atataggtac aagtgcttaa ttgatagaga tgttatacgt ctttaaatga ttccacaact
      241 tttggatgtg gaccatccat aagcggttct ctttgtcgaa cgccaccact ttggttgcca
      301 attgattcgc tatcaaagta cgatttatct tgatttgatt gttcttgaat atgttcttca
      361 ttatcagttg ttgcatattc actatccacc tctgtttttt ccattgttgc tgtatggaat
      421 tgtacgaagt tctcttcttc taatgctttg atttcttctt tcgataacgc tcgataccaa
      481 tctttcgctt ttttcgccgc aataggaaca acatcttttt caagatatcc ttcttttaac
      541 attgcattcg caatttctat cgctttttgc cgtttaatta cttcaaattc tttccatttt
      601 tcggggtaac ctttcattgt aaagggcatt tccttaacct ccaatatgtt ataattcatg
      661 ttatatacca ccataattat aaaatgaaac atattaaaca cattttttta gactcccgtc
      721 ataataactt cagacaaacg ttagtctgac ctgataaata tttaatttta aacgttcttg
      781 tctctctcac gaaaaccatt gtgttgaata ttcatcattg ctgattgaat ataattatac
      841 tgtggtaaat ctggtaatat ctttatttct aattctgtat ttaattcgaa atgcttagcg
      901 atgttttcaa atattgctaa atactcgccc attaattgtt catttattgt aagtctatct
      961 tcattagcca tggctctatt taacataaag ctaatttctt ctaatgcgaa taagctagga
     1021 taataatttt gaatcaatgt cttatcacta aataattcac cattagctgc attataaact
     1081 tgcgtcatgt tatttaattt cacagatagg ttcatagccg tgtttttctg atacacatca
     1141 tctttatatt gattttcaga aaataaataa tgaaagagcg ttgcttcttt acgaacaact
     1201 tcagccatta cattaggaag caatgcggat gcggtacgtt gtccaaggac aaataaacct
     1261 atgattgcaa tcacaatacc cattgcaacg tcaataactc ttggaaacgc aatgttaatt
     1321 gttaaattct gtgatgctaa tccgttcatt aaaataactt gtattgtaat aaaaactact
     1381 gcaatcgcat aatttgctcc caccaatgct tcagtaaaca atgctgcaat gcccattaaa
     1441 attactgcaa caggtgttgg tattgaaaac aacaatatga ctgaaagtac taacacacct
     1501 aaaatagtac ctaaacctcg tgccatacct ctctcgattg catgtatagt tgatgttcct
     1561 agtaatattg tatgtgcaga taacggtatc cagtatgctt tttcaaaatc aaacattaac
     1621 gcaataaata tcgctatcat cataatcgct gtatatctca atgtatttct aaaaacaata
     1681 gagtctaatg ttaaattttg ataaatgcgt ttactatata aaggttttcg catgtctgct
     1741 tcatacgcaa tacgcgcttc atttgcatgc accatttcat caattttcaa tatatggttg
     1801 aataaatttt gaaattcctc tgttactgtc acttcttttc gccaatatct tacattttcc
     1861 tcactattat ctagttgtgc aatgatatga tccatcattt caattaactc atctggcaat
     1921 ggtcgaattt gtttagcgtt caactctaac agttcagaat aaatcccttg ggcagatgtg
     1981 tgtaataata ataatttttg gaaacgtcta cttaatttat cgttacttga agtagaagtg
     2041 attaataatt tatcagatgc tttaaaacta tccaccgctg tttttgctac ttgtaaaaaa
     2101 gcagatttat cattataagt gtgtaacaac ttagatatga gtttaaaatc attttgaatt
     2161 gcttgttctt cagctttgtt tttagaaaat acgatcgtga ttaacaccat catagtggca
     2221 agtataccgc ctactaaaat cgcaaagcct ctatataatg cttcttcggg agctataggt
     2281 aagttaatgg gtagactgaa tgtcacaatg aagaatgtcg atgacggtcc agctattttt
     2341 aaggcagtaa atatataaaa tggtataacc gtaacaatta ataatgtcat tccaaaaacg
     2401 agtggcgttt tggctgttag cgtaccaagc atcatacata ttgcaaacgc taaattacaa
     2461 attattacag ttcgcagctt agatcgcgac ggacctttaa aaacataaat atgggctagt
     2521 gttccggttg caactaataa tccaaattgg aaattaccac ataagtaacc gattattgca
     2581 ggaataatca ttaacagtcc ctgtcttaca cctttataaa agtctaagtt aagtcgattg
     2641 atttttatga gtgatgtcaa aaagatgtca tttcgacttc tcctttcctt gtcaaaatat
     2701 ttccataatc tttttattag catagttaaa atgaatttgt tgctataaat tttaattacg
     2761 caacatatgt cattataaca ccttatgctc aattatctaa atttaaaaac ttttaaagtc
     2821 caattaaaat taatttctaa tgttatttaa catcgttgaa cctttggtcg cccacctttg
     2881 cgctagttaa aatttatatg tacttttaca agcgttacct gactaatgtt ttaaaacctt
     2941 atttctttac atatatcatt tcaataactt cattttcatc ttaaaccata tctgttttaa
     3001 aaagtttcaa tttcaacgca aaaaaagcaa taagccccaa ctatgtgaaa gcttactgct
     3061 tgtttaaatt atttttctat tgctttatca tcactaacaa ttaaaggttg atctggtttc
     3121 acatagaacc acataatcac agcgattact gctggaacga ttaacaattg gaatgtcatt
     3181 gtccaaccca aatgttctac gaatagacct gctaatagcg gactgattaa tgcacctata
     3241 ttaccccata agttcatcca ccctgatact gtaccagaga agttacgtcc taagtcagtt
     3301 gctgcagccc aactcatacc cattgaaata ccgataccac caagacctag tgataaccag
     3361 aaaatactta catataaatt tcctgtgcgt acagcaaaga taattgaaac tgcaaacaca
     3421 ataaatcctg caattgcaat tacacctcta gctacgaatt ttgaacgacc taatcctaat
     3481 acacggtcag aaattgcacc tgctgataag attaagaaga acattaataa ccaaggtaat
     3541 gaactaatgc tcatttcttt aaagttaacg tggaatactt ctgttaaata cgtcggtaac
     3601 caaattaaga ataatgtaat gataaattgt acaacaaagt attgaattgc aattgcatag
     3661 aagctaaaac gtttaaagaa atcattccat ggtggtgatg acttttcagt agctacgata
     3721 tcacgatttt ccataatgaa acgtttctcc gcttcattaa ccattctatg ttgctcaggt
     3781 aagtctttgg caataatcgc ccataataca gccattaaaa tacctactgc accaaaaatg
     3841 taaaatactg cttgccagtt aaatgcgtta acaatagcaa ttgtaactat tggtgctaat
     3901 acaggtccga aatatgatcc tgctaataat gcacttgatg ctctaccttt ttcattttta
     3961 gagaaccaaa atgaattaaa cacagcatta gaagggtaca ttggcgcctc acctacacca
     4021 aataagaatc tcactaaata aattaaaccg tggttcttaa tcatacccgt taagattgta
     4081 aatgcactcc accaaactaa tgcaattgta atcatcttac gtggaccgaa tttttcagcc
     4141 aacatccccg atggtacttg cattaatgca taacctagag agaagaatga tgccaataaa
     4201 ccaaattgtg gttttgtcat ccctaaatca tccatcattt gcttagcgat atatgaaatg
     4261 tttgctctat ccatatacgc aataacccca ataaagaaga acgcaagcgc gaacatccac
     4321 cttacgttag ttcgtttttc cttcataact attcctactt ccctaattaa ttttttaaaa
     4381 cttaccctga aacgttaaaa ttttcggaca tctggcatgt ccacccctac tttgtcacaa
     4441 aattgtacca aagttatttt ttataatatt atcagacatg gaaagcgttt acaatagaat
     4501 ctataaaaat tttattaaat tccgcttaac ttttgctatt aaaacgatat aaaggtactt
     4561 ttagttacat agtagcctat cgtttgttaa ttaaaaatac tgcaatccta gtgttttaaa
     4621 acgtatattg caggctttct tacatattcg acattcattt taaattgcat ataactacat
     4681 taaccattca ttgtgcagcg cctgttgttt agatgatcat ttattgactt tttgctctta
     4741 tagcaagcaa ttttttgatt tattgcttat aatacatttt aagacaaatg tttgtttcaa
     4801 catcgcatgc gacatcatta attattcatt tgaatttatc cagtttctat ttgtctgcaa
     4861 ttgctgaaaa ttaaaggagc ttatttttaa taatggaaca aattatcact gaatttatta
     4921 gccgttttgg gtatgcagcc atttttatat taattttatt agaaaatgta ctacctatcg
     4981 taccatcgga aattattctg acatttgctg gtcttatgtc tgttaaatca catttatcaa
     5041 ttttaacttt atttattatt gcaactatcg catcgtttat agggctgtta attttatatt
     5101 atatctgccg tttgatttca gaagaacgtc tatatcgttt tattgatcga cacggtaagt
     5161 ggattaaatt gaaaagtaag gatttaaagc gagcaaatga ttggtttaaa aagtatggcg
     5221 tatgggctgt atttatctgt cgtttcatac ctgtattacg tgtattgatt accattccag
     5281 ctggtgtaaa ccgcatgaat gttgtgacat ttaccgttat ttcattaata ggtactacaa
     5341 tctggaattt cggtttaatt ttactaggac gcactttgag cgatagcttt ggtatgttga
     5401 tgactggtct tcatacatat tcacgtatta tgtatgttgt catcattatc gcagtcattt
     5461 actttgctat acgttacatt ggcaaacgta agagagtaaa ataatattgt tgttagcata
     5521 aaggcatgaa ttttaaattt ctataaacaa aaagcagtga atcattccat ctattgaaat
     5581 gattcactgc ttttttaagt ttatttggca tactttagct gaaagtcaac tatctttata
     5641 taaagaaata ttactattaa catgatagtt atagttgtga agtaaaaggc taccaacggc
     5701 tgagaaatga taccaattaa taatgcaccg acagtcattc caatatatct cacaaaatta
     5761 aatacaccta gcactttccc ttttatatgt tcaaaccttt tagaaattat agtagacagc
     5821 aaagctggca caataccaac acaagtacct aatataaata taattactga taatataatt
     5881 acatttgatt cgtttaacca tccaaacaat aacgtaaata tagcaaatcc agttatagta
     5941 acacgaagta tcattatttc attatatttt tttgaaaatc gcttataaaa taaacttcct
     6001 aatataacac taacagtaat aggtaaaaac aaaactcctg atattccaac gcctatatca
     6061 taggtattat ttaataatgt tggtaaataa actaatattg aaaagtacgt aaaaaatatt
     6121 aaaaatccca atgtcactaa agtaataaac actttatcgg tcaaatattt ttcttcaatt
     6181 ttttcagtta attttacaat ccctttttca acatttggaa tatcaataaa aaagataatc
     6241 aaaaataata cgatagatat aacgagcaaa aaactaaaaa taccaatata atcccatctt
     6301 gcacctaaag tactccctaa gattggtgcc aaagctggtg cacatgataa aaatatttga
     6361 tagttcgcca tagcttgagc tctaccatta tctgtactta gtttggctaa taaagttaaa
     6421 gtcacgagag gaataattcc gcaaccaatt gcttgaaata accttgatat agcaaataat
     6481 ataaagttat gtgtcactgc acaaataaag ctagatatga ttactattcc caatcccgtt
     6541 aaaagtactt ttttcgaatc acgagaatct atacttctac caagtacaat ttgcataact
     6601 gcaacgataa atataaaacc gccaactgtc gcatttatcc aaaatagtgg tacttgaaaa
     6661 tcgtcatata gtctcggaat aataggcgtg tatatgtttt gaatcaatga tgcgaaaaat
     6721 gctaaaaatc ctagaatata taatctgata ttactattaa tcatttaaac catcttcttt
     6781 ttttaattct tctatactac ggtttaaaaa ctctataata actgccattt catcatcatc
     6841 aaagacttta agtacctctt catatttttt taccgatttt tcatgtaatt catcatgtat
     6901 ataagataat ttttttcctt tttgtgttaa tgaataatta acttctcttt tgttaaattc
     6961 attgtgcgat tcaactacta agtctttcgc taaaagtttt tttatcgatt tagtaattgc
     7021 tggttttgat acatttaaca tttcagacaa aaatttatta ttcactttat cattattatc
     7081 aatcaactct atgatatgaa attgtgttag agataaatct agttcctcat tttccttttt
     7141 atactgtctt cttttatcgg cattttctct ttctataata aattgttgta attttaaaat
     7201 aacttctctc ttcatatcca ctattattcc tcctcttcat tagttaaccg gttaactata
     7261 ttattgttgt cactcccatt tttgtcaaat attgttgtaa ttaacatata tttacttagt
     7321 tatgcaccat ttattccgca tttcacaaat ccacctataa tacaaactat cattttaaat
     7381 aagcagaatt taccctgtct tccacacaaa ttgttttcga cactttaatg tgcctactat
     7441 ccaaaaaagt tactcataac aaattgcgca ttttaataaa tgtagcaatg ccatttgcat
     7501 gctgcaaaaa tactcgtatt catatttatg ttttatatta ttaaagtttt ttaaatccat
     7561 atttttataa cacttgctat gtgataaaat taattttata tataaaatct taagattcag
     7621 attatttaat agcaaaggag atagtgatat ggatgtttta acaatagaac atttaacaaa
     7681 gaagataggc aacaaaacga ttctcgaaga tgtatcattt aagctgaaac gcggacaaat
     7741 agttggtctc gttggagcga atggtgcagg taaaacaact ttaatgaaag ttatattagg
     7801 ttactctagt ttccaaagcg ggaattttaa tgttattaac agcaaggaca gcaaaagcaa
     7861 tatcggcgca ttgattgaaa atccaggaat atatcctttt atgtctggat atgaaaactt
     7921 gaagttattg aatgaatcaa aaaacactca agatatcgat aaaattgtct cacaacttca
     7981 tatggatgaa tacattcata aaaaagctaa aacgtattct cttggtatga aacaaaaatt
     8041 aggaattgct atagcatttt taaataaacc tcaattcatt atcttagatg aaccaatgaa
     8101 tggcttagat ccaaaagctg tgcgagatgt acgtgaattg attgtccaaa aagcgcaaga
     8161 aggtgttact ttcttaattt cgagtcatat tttaagtgaa ttagttaaaa tcacaaactc
     8221 tatccttatt attaacaaag gtaaaattat tacagaaaca tcggaagaag aacttaaaca
     8281 atttaaagat aatgatttag aaaatgtatt actagaaatc atagaaaggg aggaccaagc
     8341 ataaaatggg aactttaatt aaacaagaat gtttcaaatt atttaaaaag aaatcaactt
     8401 ttatcgcacc tattgtcttt attctactaa tggttgctca aggttatatt gctacaaaat
     8461 acaatgaaat ttttacgcca caggaatctt tcacatctgc ttataatggt ttttcatggt
     8521 ttgcattttt attaattatt caagcaagta caatcatttc aatggaattt cattacggta
     8581 cgattaaaaa tttactctat cgtgaatatt caagaacaac tatgattgtt agcaaaatca
     8641 tcacattatt tattatttct ttaatttatt ttgttattac aattattgct tcaattgtta
     8701 ttgggtcttt attctttaat gatttaaata tatttgaaag tagcggtaat caattatctt
     8761 tattgaatca attattatta gttagtttag gcacatttgt tggcgtttgg ttagttttaa
     8821 gcttaacgtt gctattatca tctgcaacaa attcaacggg agtagccatt gctgtaggta
     8881 ttgtttttta ttttgcaagt tctattttag cagttattca aacggcactt ttagaaaaaa
     8941 tagactggct aaagtggaat cctattaata tgatgaatat tatgcttcaa acagttgaaa
     9001 aaggctttag taagtcgaca aaattagaac ttcatgaatt gtttattggt aatattgctt
     9061 atatttctat tttcttaata cttgtagtat ttattttcaa gaagaaaaat atttagtaac
     9121 ttaaagtatt aaatgtctaa atacacacat attccatcgt aattcaaaat cattttcaaa
     9181 tcccttcacc caaataatgg tgcggggatt ttttcatcca aattttggaa attccacatt
     9241 ttacatatcg taatttttta ggaaactagt gaatataaca aatccctcct ctcattttta
     9301 aaatagatat atcacttccc cacttttact taactaaact gcaacggttc ctaataccaa
     9361 aatcctgccc tctattttta tcaattcaag catacttatt gaaaaatgtt aacgttttct
     9421 tgataatcat tgtaagcgca tttattttat aaactaacgt ttgaaatata ctacaggagt
     9481 gacacgtaat gactcaaatt actgaaaaag aattaaaaaa gaagtattta gatttactat
     9541 cccaaaattt tgatactcca gaaaaacttg caactgaaat tatcaattta gaatcaattt
     9601 tagaattacc taaaggtacg gaacatttcg tcagcgattt acatggtgaa tatgaagctt
     9661 tccaacacgt attacgcaac ggttctggga acgtgcgagc gaaaatcaat gatattttca
     9721 aagagagact ttcaactaag gagcttaatg acttaactgc tcttgtctac tatccagaag
     9781 acaaattaaa attgattaaa agtgatttcc aaaattgcgg tcaacttaat gtctggtata
     9841 tcacaacaat cgaacattta attgagttaa ttaaatattg ttcttcaaaa tatacgcgtt
     9901 caaaactgcg taaagcattg ccaaaacaat acgtttatat tattgaggaa ctactgtata
     9961 aaagtaatga atatcaaaat aaaaaatcat attacgaaac acttgttaac caagtaattg
    10021 aacttaaaca ggcagatgat ttaattattg gactcgctta ttccgtacaa cgcttagtcg
    10081 tcgatcattt acacgttgtc ggtgatattt atgatcgtgg accacaacca gataaaatta
    10141 tggatacact gattaattat cattccctag atattcaatg gggtaatcat gatgtgcttt
    10201 gggttggagc ctatgctggg tcaaaagtat gcttagcaaa cttacttcga atttgtgcac
    10261 gttatgataa tttagatatt atcgaagacg cttatggcat taatttaaga ccactgctta
    10321 ctttagctga aaaatactat gacgcagata atcctgcttt taagcctaaa aaaagacctg
    10381 acaaacacga acgtttaact caacgtgaag aaagtcaaat tactaaaatt catcaagcta
    10441 ttgcgatgat tcaattcaag ttagaaatac caattattaa acgtcgtcca aatttcgaaa
    10501 tggaagaacg tcttgtgctt gaaaaggtta attatgatac aaatgaaatt acagtttatg
    10561 gtaatacata cccattgaaa gacacatgtt tccaaactgt caatcgtgat aatccagcag
    10621 aattactacc tgaagaagaa gaagtcatga ataaactatt attgtcattc caacaatctg
    10681 aaaaattacg tcgtcatatg tctttcttga tgcgtaaagg ctctctttac ttaccatata
    10741 atggcaattt actcattcat ggttgtattc cagttgatga aaatggtgag atggaatcat
    10801 ttgaaattga tggtcatact tacagcggcc aagaattatt agatgtgttt gagtatcatg
    10861 tccgtaaatc atttgatgaa aaagaaaata ctgatgactt atcgacggat ttagtttggt
    10921 atttatggac tgggaaatat tcgtcactat ttggtaaacg tgccatgact acgtttgagc
    10981 gatactttat tgcagataaa gcttctcata aagaagaaaa gaatccgtac tatcatcttc
    11041 gtgaagatgt gaatatggtt cgtaaaatgc tcagtgattt cggattaaat ccagatgaag
    11101 gacgcattat taatggtcac acaccagtga aagaaatcaa tggcgaagat cctatcaagg
    11161 ctgatggaaa gatgcttgtc attgatggtg gcttttcaaa agcttatcag tcaacaactg
    11221 gcattgcagg ctatacacta ttgtataatt cattcggtat gcaactcgtt gcacaccaac
    11281 aatttaacgc taaagaaaaa atactttccg aaggtatcga tgaactttct ataaagcgcg
    11341 tcgtagataa agaattacaa cgtaagaaga ttcgtgatac caatattggt aaagatcttc
    11401 aagctcaaat agatattttg aaaatgttaa tgcatgatcg ttacttagac taaagacgac
    11461 atttgatgca tgtatcgtgc acttatttag tggtactgtg tttacgcatt ggctacgcaa
    11521 acacgatgat aaataggcga cctagctaca ccgaaaaaaa tcccctcact attgcgagta
    11581 gtgaggggat tggtgtatct atgctaattc ctgttaactg tattataaca actttacagc
    11641 aaaaaaatgc aaaatcaact aataaattaa tgaaatgacc gacaccctcg cttctactcc
    11701 aatttaacaa aatactatat tagtactata tttttcatta acatgtatgt tacattttct
    11761 atattactta aaattacgaa gggacatttt taaatcatgc gtatcttaaa agagtccatt
    11821 attgtggcat ttgcctttgt tggtgttgtc gttggtgccg gctttgctac tggtcaagaa
    11881 attttccagt ttttcacaag tcatggcgca tatagcattt caggcattat tgtaacagga
    11941 ctattgatta ctttaggtgg aatggttgtc atgcatacag gtcatcatct aaagtccaga
    12001 aatcattctg attcaattaa ctatttctta tacccctcta ttgcaagagg ttttgatatt
    12061 attttaacaa tgtttatgtt gtctttagct attattatga ctgcaggtgg tgcgtcaacc
    12121 attcatcaaa gtttcaactt accgtattgg ctgagcgcac tcatattagt cgcctttatt
    12181 ttagcaacac tgtttctaaa attcgatcgt ttaattgctg tgcttggcgg tgttacccca
    12241 tttttaattg cgattgtcat tatgattgcg gtctactatt tcacaacaag tcatcttgat
    12301 tttactgccg ctaataatga tgctcagatt cataagcaga aatcattatc acctggatgg
    12361 tggtttgatg cgattaacta tgcaagcttg caaattgctg ctgccttcag cttcttatca
    12421 gtgatgggta gtaaagttaa atatcgtgac tcaacgttat acgggggctt gattggcggt
    12481 ttaatcatta catttttact catgatgatt aatctaggtt taatttctca attcgataaa
    12541 attaaacacg tagatctacc tacattaaaa ttagcgacac aaatgtctcc gtcaattggt
    12601 attattatgt ctgtcattat gatacttgtc atctacaata ctgttgttgg attaatgtat
    12661 gcatttgcgt cacgtttcag cgttccattc agcagacgtt acttcatcat tattattaca
    12721 atggctgtca tcacttatat tagtacattt atcggtttca tttcattaat tggaaaagta
    12781 ttccctatta tgggattgtt cggtttcatc ttactcatac ctgtactcta taaaggttta
    12841 attaagcgta ttaccggcaa atctcatatc gattaaaata tctatactat tgtaaaattt
    12901 agtacagcac ttcaaaatca aaaaagcggg cagatgaaaa gtaaaaatct tttccatctg
    12961 tccgcttctt aatagaactg ttatttccca agtatttgtt gaccagccgt taatccagtt
    13021 tctgtaattt catggccctt cacccaaact tcttcgactt gtgccccacg tgtattaaac
    13081 aagttaatga cttgttcact tgcagctaat ggcacaattg gatcatgttt ccccatagaa
    13141 agcaacacac tgacatctga taaatccttt gttgacgtta cttcaactgg gtataacggt
    13201 gcatataaca atgctttttt aaatggtgct tctgaacgca acattaagtt aatcgctata
    13261 tttgatccat ttgaaaatcc aacaagtact gctttttcaa tatcaaaatc ataacgttca
    13321 gcagcttctt taatgaacgt caacaattct tgtccacgaa atgccaaatc ttcttcatca
    13381 taaacacctt caccaagacg tttgaaataa cggttcatcc cattttctga aacttgtcct
    13441 ctaatactta acaagtgata attttcattc aatgcttcgc ctaacggtaa taaatcgaac
    13501 tcatcaccac ctgtaccatg caataatatt agtgttggcg caccattttg tccttctcta
    13561 aaaatatgtt ccatcacaaa tcctcctcat tccaattaac catgttgacg cttcgtatta
    13621 aaaggtctaa cttccgattc aatatattct cttttatttt ctaaaaatgg tggtaaggat
    13681 aacccttcgc ctaatgtttc ataaggttca tcttccataa atcctggtcc atctgttgaa
    13741 atttctatta aaatatgccc cacacgtgca tataatgctt caaaatagaa acgattaacg
    13801 atgcctgagt tattaatacc tacctcttta tatttcgttg cccacgcttc tattgcatca
    13861 tgatctttca cacgaaatga cacatgatgt acctcaccat aaccttgacg tgctgctggc
    13921 cctttatcat cttttattaa gattacctgg ccaccattgc ctccttcgcc aacttcaagt
    13981 aatgcgacat tatcttcatg cgcaatagtt gtcataccgt aaacagtctc taaaatattt
    14041 ttaaagtcgt caaaataact tactttaatt tcaatggggc ctaatccata aatcgcttta
    14101 tctactggaa ccggtccatt cttccaaggt acaccaggtg ctaccccttc atttaactca
    14161 tctgaaatta attgatacac ttggccatcg acttcttcaa atggcaacac ttttttacca
    14221 aataattctt gaataccttc gtgtttaaca ccaaactcat caaagcgctg ttcataatat
    14281 gttaatgcgt catcgttagg cactctaaaa gacggtcttg taatggaatt tgttcctgcc
    14341 tgcccttttg taatatttgg aaaatcaaag aacgtcatgt ctgtacctgc cgaacctaca
    14401 tcatctgcaa aaaaagtatg atacgtataa atatcatctt gattgactgt ctttttaact
    14461 aaacgcatgc ctagtacttc tgtaaaaaat ttataattac gttctgcatc atctgtcatt
    14521 gcagtaacat ggtgaatacc tagtaattca tgattattta tcattttata taccctcttt
    14581 atatttttag tgcatttatc tcgaaatcga gatactttaa tgtaaatttt tagtgcacgc
    14641 cttacacttc tgtagattgt gcacttagtt tcttaaacgc ttgttgtaag attgttaatt
    14701 catcctttgt taacacatca aacgcttttg ttaatgtctc agcatgctta gggaaaatat
    14761 ctgccatttg actttgacct ttttcagtta aacaagccat atatacacgt ttatcatctt
    14821 tatccttttc acgtgtaatc caacctttgt cctctaattg acttacaaca tatgaaatgc
    14881 tgctacttgc aattaatacg cggtctctaa tacgttgaat tggttgcgga cctttattat
    14941 aaagcaactc gagcactgca aattcagtaa tatttaagcc atatcgcttt acgtcttctt
    15001 ttgtaatttg ctctaatgtg tctaacgccc tattcattcc gacaaaaaca ttgagagatt
    15061 gtttcgttcg atccatatga tcaccaactt ttttatttta tttcgatttc gagataaatt
    15121 atatagctga atagatttaa tgtcaagata cgtgttttca tcttcatttc aaactgtgct
    15181 taataaaaac gactacatag atgtggcaat gtcgtcacat gactgtcatg cacctttgac
    15241 ttcgtttaag ttaaaataga atagggaaat actataatca atcatgatag gagttgatat
    15301 gtatgagtaa ccttgaaatc aaacaaggcg agaacaaatt ctatattggt gatgatgaaa
    15361 ataatgcttt agctgaaatc acataccgtt ttgtggataa taatgaaatt aacattgatc
    15421 atacaggcgt atctgatgaa cttggtggtc aaggtgttgg caaaaaacta cttaaagcag
    15481 ttgttgaaca cgctcgagaa aataatttga aaattattgc ctcatgttca tttgccaaac
    15541 atatgttaga aaaagaagat tcatatcaag atgtatatct tggttaaata aattttgcga
    15601 tggtgcaact gtaccatcgc ttttttgttc gcatcaatat aaaattgcaa aacgactagt
    15661 tattttgcat ttcaaacttt atgctatcaa ttcattttct tttctggtat actttttata
    15721 ctaacttata gaaaaataga aaaagaggcg atgcataatg gcatttcacg ataaaacagc
    15781 aacacaagtg acaaacattg tactgaacgt aagagactta gacttaatga caacatttta
    15841 caaaaacatt ttaggactat ccgttaaaag ttctgacgat aatacaaccg tattatctgt
    15901 tgggactggt ggccatactc taacgttaca tttattagaa gacggccgtc agacttcccc
    15961 acgtgaagca gggctttttc atatagcatt tttattacca actactgagg atctagctaa
    16021 cttcttatat ttcgtggcac aaaaaaatat gggcatcggc gctggtgatc atttagtaag
    16081 tgaagcttta tatttcaacg accccgaagg taatggtatt gaagtctatc gcgatagacc
    16141 ttcatcttca tgggaatggc aaaatggcaa agttaaaatg gatactttag aagttgatag
    16201 ccaaacctta ttaacacatc gtactgatga aggttggcaa ggaatgccag caaaaggcat
    16261 gattggacac ttacatttaa aaacacatga tttagacgca gcatatcaat gttacattga
    16321 acaattagga ttccaacatg tgtctgattt tccacgtgcg ctatttatgt caacgaatca
    16381 ttatcaccat catattgctg ctaatacttg gcaatccaat caagcaagac aaaacaattc
    16441 acaaagttat ggattaacac atgttgatat ttatcaaccc gacgcctcaa cacatacatt
    16501 tacggcacca gaagggtttg acatcacagt tcattcaaat actgaccttg ttccagaaaa
    16561 ataatcatcc atactttatt aatacaattt tttcacgcaa aagcctgcga ttcatttatg
    16621 taatcgcagg cttttttatt gctaactttt taattttata tatttagaca tacggtattc
    16681 tatttattct tttggtccaa cccattcaat aacatcttca taagcttggc gtgttttatt
    16741 tttcggtggc tcttgttgtc tgtagccaaa tgcgaccatc actgataaac caaattgctc
    16801 agtatctaag atccctttat ttgctaaaat gtctgtcact gtatccagac taaaaccttc
    16861 catcggacat gaatcaatac ctaacaatgc ggctgtcgtc atcatattgc ctaatgcaat
    16921 atacgtttgt ttacttgacc agtcatacaa ggcttgatca ttatcagaaa tatggaaatc
    16981 tgcttggaat gcatcgaatt tttgttcaac agctggaatc gtttgtgcct catatttttt
    17041 aatatctctt aacatatgtt gtacatacgg tgatcttgac gttacatttt tacgcgcaaa
    17101 aattagcaca aaatggctcg ctgtatctaa ttgttttgct gcgccccaac tgtgcgcttt
    17161 taattcatca cgtaacgctt gatcttgaat cacgacaaac ttccaaggtt ctaacccaag
    17221 agaacttgga gacaatctac ctgactctaa tattgtttca aaatcttctt tcgaaacttt
    17281 cttttgtgga tcgaattgct tagtcgcatg tctgaaatga aatgcatcca taattgtttg
    17341 attcatattg ctcattgaac atagcctccc atttttatat ttattgctat acaaattata
    17401 cttcatgttt taaaaagact aaagtaatcc gtttgcaatt cactataact tgtgcacatg
    17461 ttataagttt tctgcaataa aacacacttt tgaattttgg gaaatttagt gttataatat
    17521 atttgtgata tttttcacaa ttaaattgtg gcgtgatttt aatgtgcata atctttaatt
    17581 tcactcattt acttttcaat cttttaaaag cgaggttttt aataatgaca aaaattatgt
    17641 tctttggtac gcgtgattat gagaaagaga tggcattaaa ttgggggaaa aagaataatg
    17701 tcgaagtaac tacttctaaa gagctattat caagtgctac agtcgatcaa ttaaaagatt
    17761 acgatggcgt aactacaatg caatttggta agttagaaaa tgacgtttat cctaaattag
    17821 aatcttacgg tattaaacaa attgcacaac gtactgctgg atttgatatg tatgatttag
    17881 atttagctaa aaaacacaat attgtgatat ctaacgttcc tagttattca cctgaaacaa
    17941 ttgcagagta ttctgtttct atcgccctac aattagtgcg tcgcttccca gatattgaac
    18001 gccgtgtaca agcacatgat tttacttggc aagcagaaat catgtctaaa ccagttaaaa
    18061 atatgactgt tgcaattatt ggtacgggtc gtatcggtgc tgctacagct aaaatatatg
    18121 caggatttgg tgctacaatt acagcttatg acgcctatcc taataaagat ttagactttt
    18181 taacttataa agatagtgtt aaagaagcta ttaaagatgc cgatattatt tctttacatg
    18241 ttccagcgaa caaagaaagc tatcatttat tcgataaagc aatgtttgat catgtgaaaa
    18301 aaggtgcaat cttagttaac gcagcacgtg gtgcagtcat caatacacct gatttaatcg
    18361 ctgcagtgaa cgatggtact ttgttaggtg ctgcgattga tacttatgaa aatgaagcag
    18421 catacttcac aaatgactgg actaataaag acattgacga taaaacatta ttagagttaa
    18481 tcgaacatga aagaatttta gtaacaccac atattgcttt cttctctgat gaagcagtac
    18541 aaaaccttgt tgaaggtggt ttaaacgcag cattatctgt cattaacact ggtacatgtg
    18601 aaacacgttt aaattaatat atatatttaa aagcaaaggc gcgccatgtg cctttgcttt
    18661 ttattttgta tatctgtatc aaaatcgatt tgattaaaat ccgctttatt ttatcatcta
    18721 ttcaaatgat tttagtgcgc ttattttact atggcattat tgcctaacct gtttgagata
    18781 tattaatatt tgatgattcg tattcaaagt tttcatttaa catgtattta gtatcatgat
    18841 agctgcttca ttgatgatat actacctact ttttcaacac ttcttgttcg ataatcgtta
    18901 atacaccctg cttatcatta cttggagcga cagcagaggc aatattgaac aattcttcat
    18961 catgactatt ttccatcaca taactatgct tagcgaacgc taacatatct ttatcattat
    19021 tcgcatctcc gaaggccatg agctctgaag gagacatttc ccatttatct aacaatcgtt
    19081 ttaatgcctg ccctttagtc atgtttggca taataatatc aatgctatcg ttcccacttg
    19141 agacaagttt aatatcattg ctgaattgcg ttgctacttc ttcgtcaaca ttcggatgcg
    19201 tctcacgatt aatattaaat gctattttga cataatcatc ctcaggtaat tgctgtagac
    19261 tgtcaatttc ttttaactga tgataataaa atctcgtatc ttctttaaac gcttcagaag
    19321 tatgttttaa aatatacgcg cttttcaaac cacagatgac gagttgatca atactttgct
    19381 tcatatttaa gtaatcgaca acctgttgaa acacctgacg attaaagctt ttataattat
    19441 ataactcatt gccattataa ataactgcac cattttcaga aataaaatac atatctctat
    19501 ccccgaaaat agacttcaac ttcgcatatt gattgccact cgcagcaata aatgtaatat
    19561 ctctatttct aagttcagta aaaatcgctt caaatctaag tttatcatat gtcttttttg
    19621 aatctaaaaa cgtcccgtcc atatctaccg caatagcctt aaccatgatt atcctcctca
    19681 ttgctctttt agtcgtcttc attatatcca tctcatgaca agcaatgaag tttgtcatac
    19741 cacaatgaac ctatattcac attttgaaat tgctttcata tatgcaaaac acattacact
    19801 ggatatagca ttcagaggcg aacataaatt tcagaatatt gtaaaaatat cacactatat
    19861 ttatcattta gacaaaatat agtgtgatat actaatttat aacatctata cataatgtga
    19921 aagaattttt aaaaatacaa ttaaatatcg gtgtaataaa tccctttata cataggagtt
    19981 gtatacatga aactaaataa ctattcttta aaagttaaaa acaaacaact agttgacaat
    20041 tgtgatttaa atttctatct tggtcagatc aatcacattg ttggtaaaaa tggtgtagga
    20101 aaatctttat tagctaaaga tttcttacta aataatagtg gaaatatccc taagtccatt
    20161 tctcaaaatg taaccttaat atcaagttca tcaaatattc ctaatgatat aacaaaagat
    20221 tttttattat cattgttaaa atcaaaattt gaaaacaatc gacaaacatt cgataagatt
    20281 tataacatac taaacatcga agcaataccg tctaacgtac tactaaaaaa cttgagtgat
    20341 ggtcaaaaac aaaagcttaa attattaagt ttcttattgg aagatcatga tttaatcata
    20401 ttagatgaag ttacaaacgc tttagacaag aaaacagtta atgaaattta tgaattttta
    20461 aatgatttta ttcaaagcca tcaaactaaa actattatca atattacaca taatttatcc
    20521 gatttaagtg ctttgccggg taaatacttt atttttaaag accttcaaat agaagagtac
    20581 caatcaaaag aagaagtcat aaatgattac attaatttat aattgaggtg tccactttga
    20641 aattagaatt taaaaaaagt atttccaata aaattattta cacgcttgtt gtgctattca
    20701 cattcctttt cttacttggg tattttttac caataggtat agacaaagtt aaaagtttaa
    20761 gctatagcca gtttttcttc agttcatata ctgttgcaac gcaattaggg ttcttactat
    20821 tttcatttgt tattgcatat tttattaata aagagtattc gaataaaaat atcctgtttt
    20881 ataaattgat tggcgataat atctttacct tcttctataa aaaggtagca gtgttatttt
    20941 ttgaatgttt agtgttcata attctaagta tcactattat ttcaattata tattcggatt
    21001 tctcacatta cttactatta ataatcttat tttcactcgt tatcttacaa tatatcttag
    21061 ttgttggaac gattagtatg gtttcaccta acattttaat cagcttaggt atcagcattg
    21121 tatattggat tggttcagtc atcttagttg ccattaataa aaatatattt ggtatcgttg
    21181 ctccatttga ggcgagcaat acgatgtatc gtgccgtaga aaaaatattg aataacgagt
    21241 caacatttat gtgtcctact gaaattataa atatagtatc attcttcgtt cttctattta
    21301 tagttaatac tattgtctta ttactaagta gaaagagatg gttgaaaatc ggtatgtagt
    21361 ataaaaaagg agcggtatac attgccgctt ctttttatta tgtattttta caatctccac
    21421 caatgacacg tcgtcattgc tacctcatac caaacaaaaa acagtctcgc aatcaagact
    21481 gttttccact caatatattc atccattagc gtaatagatt atttgacttc tgtagctaca
    21541 aagattttac gtttttccca aacgcctgtc ttttcattgt aatcatcaca agtaattaat
    21601 gttaattgtt tatctttacc tttttgttca tctagaactc ctacatctgt aggcttaaca
    21661 tctcttatac ttgtcatttt atacttacgt gtttcattac caactttaaa gtacaccata
    21721 ctaccttttt tggctgcttt aagatttgta aattgatagt tcggacggtc aatgaaagtg
    21781 tgtcctgcaa ttgaaatatt ttgatcatct agtgattcat tttcttctgc aaagcttaca
    21841 cctctattta attgttcagg tgttgctggt cctggatata ctggttcttt aatatcagca
    21901 tctggaattt caatatagcc tgccactttc gatttatctt tcggaatttg aggtttagct
    21961 tgctgctttt tatctttact cgcctgttct tttacatttt tatcatattg ttcaatcttt
    22021 tcatctttat ctttatcgtg aagataatta tcgatatgtg gtttagcaaa caaatatgct
    22081 gccactagga taagtaccac accagcgatt gtcattaatc gatttgtcca ttttttcata
    22141 cgttaaggct ccttttatac atttcacaat gcctgttaac atttcatata gtataccatt
    22201 aatttcaaaa tgactcatag caattcattt tatactataa aatttacatg tatactttta
    22261 cgttagattt cattacacat atttgcattc aaataacgaa acgcttttaa taattactaa
    22321 gggggaattg atatgattag atacgctaaa aaagaggatt taaacgctat attagcgata
    22381 tacaatgatg ccattatcaa tactacagct gtttatactt atgaaccaca aaccatagac
    22441 gaacgtgtcg catggtttga aacgaaacaa cgtaagcatg agcctatctt tgtatttgag
    22501 gaaaatggaa gtgtcttagg gtttgcgacg ttcggttcat ttagaccttg gccagcatac
    22561 ctatatacaa tcgaacattc tatttatgtc gatgcttcag ctagaggaaa aggtattgct
    22621 agtcaattac tacaccattt aattgtggaa gcaaaagcta aaggttatcg tgcgctagtt
    22681 gcaggcattg atgcttccaa caaagcgagt attcagttgc atcaaaagtt tgcttttaag
    22741 catgccggca cactgaccaa tgtaggtttt aaatttaata gatggttaga tttagcattt
    22801 tacgaattag atttacaaga ctagtaatgt ttgaatcaca taatataaac aagacaacca
    22861 tgttaattcc cttaacataa caagccaaca tataaaattt taaacttctc aggggagtgg
    22921 g
//
LOCUS       DANRDI010000030        21986 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.030, whole genome shotgun sequence.
ACCESSION   DANRDI010000030 DANRDI010000000
VERSION     DANRDI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21986
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.030"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..647)
                     /locus_tag="PZ377_000885"
     CDS             complement(<1..647)
                     /locus_tag="PZ377_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDM6357771.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFS"
     gene            1048..1932
                     /gene="ylqF"
                     /locus_tag="PZ377_000886"
     CDS             1048..1932
                     /gene="ylqF"
                     /locus_tag="PZ377_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDM6357772.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            1916..2683
                     /locus_tag="PZ377_000887"
     CDS             1916..2683
                     /locus_tag="PZ377_000887"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDM6357773.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            2792..3958
                     /gene="sucC"
                     /locus_tag="PZ377_000888"
     CDS             2792..3958
                     /gene="sucC"
                     /locus_tag="PZ377_000888"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDM6357774.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            3980..4888
                     /gene="sucD"
                     /locus_tag="PZ377_000889"
     CDS             3980..4888
                     /gene="sucD"
                     /locus_tag="PZ377_000889"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDM6357775.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            5115..6233
                     /locus_tag="PZ377_000890"
     CDS             5115..6233
                     /locus_tag="PZ377_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDM6357776.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            6261..7505
                     /gene="fmhC"
                     /locus_tag="PZ377_000891"
     CDS             6261..7505
                     /gene="fmhC"
                     /locus_tag="PZ377_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDM6357777.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSK
                     KNKSKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPK
                     YNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEEL
                     IGDFIKPVRPILYKFAKLIYKV"
     gene            7678..8550
                     /gene="dprA"
                     /locus_tag="PZ377_000892"
     CDS             7678..8550
                     /gene="dprA"
                     /locus_tag="PZ377_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDM6357778.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            8724..10799
                     /gene="topA"
                     /locus_tag="PZ377_000893"
     CDS             8724..10799
                     /gene="topA"
                     /locus_tag="PZ377_000893"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDM6357779.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            10955..12262
                     /gene="trmFO"
                     /locus_tag="PZ377_000894"
     CDS             10955..12262
                     /gene="trmFO"
                     /locus_tag="PZ377_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDM6357780.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            12679..13575
                     /gene="xerC"
                     /locus_tag="PZ377_000895"
     CDS             12679..13575
                     /gene="xerC"
                     /locus_tag="PZ377_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDM6357781.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            13572..14117
                     /gene="hslV"
                     /locus_tag="PZ377_000896"
     CDS             13572..14117
                     /gene="hslV"
                     /locus_tag="PZ377_000896"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDM6357782.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            14183..15586
                     /gene="hslU"
                     /locus_tag="PZ377_000897"
     CDS             14183..15586
                     /gene="hslU"
                     /locus_tag="PZ377_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDM6357783.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            15611..16384
                     /gene="codY"
                     /locus_tag="PZ377_000898"
     CDS             15611..16384
                     /gene="codY"
                     /locus_tag="PZ377_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDM6357784.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            16435..16527
                     /locus_tag="PZ377_000899"
     CDS             16435..16527
                     /locus_tag="PZ377_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357785.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            16726..17493
                     /gene="rpsB"
                     /locus_tag="PZ377_000900"
     CDS             16726..17493
                     /gene="rpsB"
                     /locus_tag="PZ377_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDM6357786.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            17527..17640
                     /locus_tag="PZ377_000901"
     CDS             17527..17640
                     /locus_tag="PZ377_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357787.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            17675..18556
                     /locus_tag="PZ377_000902"
     CDS             17675..18556
                     /locus_tag="PZ377_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDM6357788.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            18693..19415
                     /locus_tag="PZ377_000903"
     CDS             18693..19415
                     /locus_tag="PZ377_000903"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDM6357789.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            19434..19988
                     /gene="frr"
                     /locus_tag="PZ377_000904"
     CDS             19434..19988
                     /gene="frr"
                     /locus_tag="PZ377_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDM6357790.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            20361..21131
                     /locus_tag="PZ377_000905"
     CDS             20361..21131
                     /locus_tag="PZ377_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDM6357791.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            21138..21920
                     /locus_tag="PZ377_000906"
     CDS             21138..21920
                     /locus_tag="PZ377_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDM6357792.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT         8350 a         2975 c         4189 g         6472 t
ORIGIN      
        1 taactgaaat aaaaattgct aaatagtgtt aaggctatcg caacaatgaa aataccgatt
       61 ttgcgttgtt gaaaatatct ttccaaacca agaatcgata atggcaataa atataataaa
      121 tttccataaa atgaccaagt aaaattaaag tatataacga cagttgacat gccgtataaa
      181 atcgtagcga tcatatttgc tgagcgttta aagtgtaata ttttaaataa gtagaaggtc
      241 acgacaaatg ttatgatagc tcgtatcata gccataataa gttggtttgt cggccaaaaa
      301 tgtattgtcg tcggattaaa tataccaacc gtttctccta ttttaatgaa tagaaaattt
      361 agccacatta aaggtgacag cgaataataa tatgatagtc ctttcatata atcgccacct
      421 aatccaaacg atgcatcata taaactagaa aaactacgta gatgttcata caaatacatt
      481 tgaaatggca tcatttgacg gaatccatct ccagccccgc taaaaacagt accattcaca
      541 atataatcat agatatgagt agaaaataaa ataagcgtta atattacact aatgaaagtt
      601 ataacaaaga attgtttgac gtttgaattt agccactttt ttaacacaac attatcctca
      661 actttcaaat ttaaaattaa gtttaactga aactaaagtt aatgaggttc ttgataggta
      721 aagacgaaga tgactgtgga acagatacct tatcatagtt acttaaactt tggatcattt
      781 tcagtttatc attaaacaaa tatattgaat aataaaaatg tcatactgat aaagatgaat
      841 gtcacttaat aagtaactta gatttaacaa atgatgattt ttaattgtag aaaacttgaa
      901 ataatcactt atacctaaat ctaaagcatt gttaagaagt gtgacaatgt taaaataaat
      961 atagttgaat taatgaattt gttctataat taacaggtta tagattttaa taatgagaaa
     1021 agaattgacg aaagtaaggt gaattgaatg gttattcaat ggtatccagg acatatggcg
     1081 aaagccaaaa gagaagtaag tgaacaatta aaaaaagtag atgtagtgtt tgaactagta
     1141 gatgcaagaa ttccatatag ttcaagaaac cctatgatag atgaagttat taaccaaaaa
     1201 ccacgtgttg ttatattaaa taaaaaagat atgtctaatt taaatgagat gtcaaaatgg
     1261 gaacaatttt ttattgataa aggatactat cctgtatcag tggatgctaa gcacggtaaa
     1321 aatttaaaga aagtggaagc tgcagcaatt aaggcgactg ctgaaaaatt tgaacgcgaa
     1381 aaagcgaaag gacttaaacc tagagcgata agagcaatga tcgttggaat tccaaatgtt
     1441 ggtaaatcca cattaataaa taaactggca aagcgtagta ttgcgcagac tggtaataaa
     1501 ccaggtgtga ccaaacaaca acaatggatt aaagttggta atgcattaca actattagac
     1561 acaccaggga tactttggcc taaatttgaa gatgaagaag tcggtaagaa gttgagttta
     1621 actggtgcga taaaagatag tattgtgcac ttagatgaag ttgccatcta tggattaaac
     1681 tttttaattc aaaatgattt agcgcgatta aagtcacatt ataatattga agttcctgaa
     1741 gatgcagaaa tcatagcgtg gtttgatgcg atagggaaaa aacgtggctt aattcgacgt
     1801 ggtaatgaaa ttgattacga agcagtcatt gaactgatta tttatgatat tcgaaatgct
     1861 aaaataggaa attattgttt tgatattttt aaagatatga ctgaggaatt agcaaatgac
     1921 gctaacaatt aaagaagtta cgcagttgat taatgcggtt aatacaatag aagaattaga
     1981 aaatcatgaa tgctttttag atgagcgaaa aggtgttcaa aatgccatag ctaggcgcag
     2041 aaaagcgtta gaaaaagaac aagctttaaa agaaaagtat gttgaaatga cttactttga
     2101 aaatgaaata ttaaaagagc atcctaatgc tattatttgt gggattgatg aagttggaag
     2161 aggaccttta gcaggtccag tcgttgcatg cgcaacaatt ttaaattcaa atcacaatta
     2221 tttgggcctt gatgactcga aaaaagtacc tgttacgaaa cgtctagaat taaatgaagc
     2281 actaaaaaat gaagttactg cttttgcata tgggatcgcg acagctgaag aaatagatga
     2341 atttaatatt tataaagcta ctcaaatcgc catgcagcga gctattgatg gattatcagt
     2401 acaaccaacg catttattga tagacgcgat gacgcttgat aatgcactgc ctcaagtatc
     2461 tttaatcaag ggtgatgcaa gaagtgtgtc cattgcagct gcaagtatca tggcaaaggt
     2521 ttttcgtgat gattatatga cacagttatc taaagattat cctgaatatg gttttgaaaa
     2581 aaacgcgggt tacggtacca aacaacattt actagcaatc gatgatattg gcattatgaa
     2641 agagcataga aaaagctttg aacctataaa atcgttactg taaatcgtaa aattcaatta
     2701 aatgataaaa aagtagcatt taagtttaca atagcgctta cattttttat aattgtaagg
     2761 gaacttaacc taagtaacag gaggatggaa gatgaatatc cacgagtatc aaggtaaaga
     2821 aatatttcgt tcaatgggcg ttgcagttcc agaaggacga gtagcattta ctgctgaaga
     2881 agcggtggag aaagcaaaag aattaaattc tgatgtttat gttgtaaaag cacaaattca
     2941 tgctggaggt agaggtaaag caggcggagt aaaaattgct aaatctttat ctgaggtaga
     3001 aacatatgca aaagaattat tagggaaaac tttggtgaca catcaaactg gtccagaagg
     3061 taaagaaatt aagcgtttat atatcgaaga aggttgtgct attcaaaaag aatattacgt
     3121 tggattcgtt attgatcgtg cgactgacca agtaacattg atggcgtctg aagaaggggg
     3181 cactgagatt gaagaagttg ctgcgaagac tcctgaaaag atcttcaaag aaactatcga
     3241 tccagtaatc ggactttcac cattccaagc aagacgaatt gcgtttaata ttaatattcc
     3301 taaagaatct gttaacaaag ctgctaaatt cttattagca ctttataatg tattcattga
     3361 aaaagattgt tcaatcgtag aaatcaaccc attagttaca acagctgatg gtgatgtatt
     3421 ggcattagat gctaaaatta attttgatga taatgcatta ttcagacata aagatgttgt
     3481 agaattacgt gatttagaag aagaagatcc gaaagagatt gaagcgtcta aacatgattt
     3541 atcatacatt gcattagatg gtgacatcgg atgtatggtt aatggtgcag gtttagccat
     3601 ggcaacaatg gatacgatta atcatttcgg tggaaaccca gccaatttct tagatgcagg
     3661 cggaagcgct actagagaaa aagtaactga agcatttaaa atcattttag gtgatgaaaa
     3721 tgttaaaggt atttttgtaa acattttcgg tggcattatg aaatgtgatg ttatcgcaga
     3781 aggtatcgtt gaagctgtaa aagaagtaga tttaacttta ccactagttg tacgcctaga
     3841 aggtacaaat gttgagttag gtaaaaaaat cttaaaagac tcaggattag caattgaacc
     3901 agcagcaaca atggctgaag gtgcacaaaa aattgttaaa ctagtcaaag aagcataagg
     3961 aaaggatggg agcactaaga tgagtgtatt tatagataag aatactaaag taatggtaca
     4021 aggtattaca gggtctactg cccttttcca tacaaaacaa atgcttgatt atggtacgaa
     4081 aatagtagca ggtgtgacgc ctggtaaagg tggtcaagtt gttgaaggcg ttcctgtttt
     4141 caacactgtt gaagaagcta aaaatgaaac tggggcaacg gtttcagtca tttacgttcc
     4201 agcaccattt gctgcagact caattttaga agcagctgat gcagacttag atatggttat
     4261 ttgtatcact gaacatattc ctgtattaga catggttaaa gttaaacgct acttacaagg
     4321 tagaaaaaca cgtttagttg gtccgaactg tccaggtgtg attacagcag atgaatgtaa
     4381 aattggtatt atgcctggct atattcacaa aaaaggtcat gttggtgtag tatctcgttc
     4441 aggtacatta acatatgaag cagtgcacca attgactgaa gaaggtattg gtcaaactac
     4501 agctgttggt attggtggag acccagtcaa cggaacaaac tttattgatg ttttaaaagc
     4561 attcaatgaa gatgacgaaa cgaaagcagt tgttatgatt ggtgaaatcg gtggtacggc
     4621 tgaagaagaa gcagctgaat ggattaaagc gaatatgaca aaaccagttg taggctttat
     4681 cggtggacaa acagcacctc ctggaaaacg tatgggacat gctggtgcaa tcatttcagg
     4741 tggtaaaggt actgctgaag agaaaattaa aacattaaat agttgtggtg tgaaaacagc
     4801 ggcaacacct tcagaaattg gttcaacatt aattgaagct gctaaagaag caggtattta
     4861 tgaatcatta ttaactgtta ataaataaag ttaaaagatg atataaatgg ctatagccca
     4921 ttccatactt tataaaagta tcggaaatgg gctatttgtc gtgcgatgaa ttttagtaca
     4981 cttttcatat tttggaaaga caagttaatt attttacctc ctacaatagc tttataaaac
     5041 aaagaaaatt ttaattaaaa tatatagcgc actatgttat aatgtttgta tattattgta
     5101 aggagtgttt catcatgaat aaacaacaaa gtaaagtacg ctattcaatt agaaaagtta
     5161 gtattggaat tttgtcaatt tcaataggta tgtttttggc attgggtatg tcgaacaaag
     5221 catatgcaga tgaaattgat aaatctaaag attttacaag agggtatgag caaaatgtat
     5281 tcgcgaaatc agagttaaat gctaataaaa atacgacaaa agacaaaata aaaaatgaag
     5341 gtgctgttaa aacatcggac acaagtttaa agttagacaa caaatcagca atttcaaacg
     5401 gaaatgaaat taatcaagat ataaagattt caaatactcc gaaaaactca agccaaggta
     5461 acaatctagt tattaataac aatgaactta ctaaagaaat taaaattgca aacttggaag
     5521 ctcaaaattc taatcagaag aaaacgaata aagttactaa taattacttt ggttactaca
     5581 gttttagaga agctccaaaa acacaaatct atactgtaaa aaaaggagac acacttagtg
     5641 ctatagcatt aaaatacaaa actacagttt caaatattca aaatacaaat aatatagcaa
     5701 atcctaattt aatatttatt ggtcaaaaat taaaagtgcc aatgacacca ttagtagaac
     5761 caaaaccaaa aacagtgtct tcaaataata aaagtaatag taatagcagt acattaaatt
     5821 atttgaaaac attagagaat agaggatggg atttcgacgg tagttatgga tggcaatgtt
     5881 tcgatttagt taatgtatat tggaatcatc tttatggtca tggattaaaa ggatatggag
     5941 ctaaagatat accatatgca aataatttta atagtgaagc taaaatttat cacaacacac
     6001 caactttcaa agctgaacct ggggacttag tggtttttag tggaagattt ggtggaggat
     6061 atggtcatac agctattgtc ttaaatggtg attatgatgg aaaattaatg aagttccaaa
     6121 gtttagatca aaactggaat aatggtggat ggcgtaaagc agaggttgca cataaagttg
     6181 ttcataatta tgaaaatgat atgattttta ttagaccatt taaaaaagca taatttaaat
     6241 cgaaggcagg acattgaaat atgaaatttt caactttaag tgaagaagaa tttaccaact
     6301 acaccaaaaa gcacttcaaa cattatacac agtctataga attatataat tatagaaata
     6361 aaataaatca tgaagcacat attgtgggag tgaagaatga taaaaatgaa gttatagctg
     6421 catgtttatt aacagaggca cgaattttta aattctacaa atatttctac tctcatagag
     6481 gtcctttact tgattatttc gatgctaaat tagtttgtta cttttttaaa gaattatcta
     6541 aattcattta taaaaataga ggagtattta ttcttgttga tccatattta atagagaatt
     6601 taagagatgc aaatggtagg ataataaaga attataataa ttcagtgata gtaaagatgc
     6661 tagggaaaat tgggtatctc catcaaggtt atacaacagg atattcaaat aaaagtcaaa
     6721 ttaggtggat ttctgtattg gatttaaaag ataaagatga gaatcaactt ttaaaagaaa
     6781 tggaatacca aactagaaga aatataaaaa agactattga gattggtgtt aaggttgaag
     6841 atttatctat tgaagaaaca aatcgatttt ataaattgtt tcaaatggct gaagaaaaac
     6901 atggttttca tttcatgaat gaagattatt ttaaacgaat gcaagaaata tataaagata
     6961 aggcaatgtt aaagatagct tgtataaatc ttaatgaata tcaagataaa ttaaaaatac
     7021 aattattgaa aatcgaaaat gaaatgatga ctgtgaacag agcattaaat gaaaatccaa
     7081 attctaaaaa aaataaatca aaattaaatc agttaaatat gcaattatct agtattaata
     7141 atagaattag taaaaccgaa gaactaatat ttgaagatgg acctgttttg gatttagctg
     7201 ctgctttatt tatatgtact gatgatgaag tttattatct atcaagtgga tcaaatccga
     7261 aatataatca gtatatgggt gcatatcatc tacaatggca tatgataaaa tatgcaaaat
     7321 cacataatat taataggtat aatttttatg gaataacagg cgtctttagt aatgaggcgg
     7381 atgattttgg tgttcaacaa tttaaaaagg gttttaatgc acatgttgaa gaattaattg
     7441 gtgatttcat caaaccagta agaccaattc tatataaatt tgcaaaactt atttataagg
     7501 tttaattata aagtatgttg gaaattgaaa ttttaaattc tttccaacat acttttcact
     7561 ttttaagtca gtatttttac tccttaaaat aacattacat cgacaatgtt tttgtattag
     7621 aaatgtaaaa caaaaataga atatgatttt gtatgattac aaaaaggagt tgatttattg
     7681 attaaactat ttttgcttaa gttatactgg gcacactttt cgactaaaca aattcatcaa
     7741 tttttaatgg catatcctaa tgtaattaaa gaggagggaa gaaaaaaaga tagttattta
     7801 tgtgaatggg tgaataggga agaaaatgtt catttattac gtaaatacta tgcttttata
     7861 aaacttgatc ataacgatat tattaaagaa ctgcagaaat taaaagtaag ttacattaca
     7921 tatatggatt ctgaataccc agtgctatta aaagaaatat atcaatttcc attacttctt
     7981 ttctataaag ggaacatcaa attaataaat aatatgcatc atttggcagt agtaggtgca
     8041 agagattcta caagttatac ccaacagtct ttagaatttt tattatcaaa tgataaaagc
     8101 aaatatttaa caattgtttc cggccttgct caaggagctg atgcaatggc acatcaaata
     8161 gctttaaaat acaatctccc tacaattgca gttttagcct ttggccatca aacacattat
     8221 cccaaaagta cattagcatt aagaaataaa atagaagaaa aaggtttagt tatatctgaa
     8281 tatccaccac atacaccaat cgctaaatat agatttcctg agcgcaatag aattatcagc
     8341 ggtttgtcaa aaggggtttt aattactgag gctaaggaac aaagtggcag tcacatcacg
     8401 atagattttg cattagagca aaatagaaat gtttatgttt tacctggatc tatgtttaat
     8461 cctatgacaa aaggtaattt attacgtatc caagaaggtg ctaaggtagt attaaacgct
     8521 aatgatatat ttgaagacta ctatatttaa aactaaaaat aacgagttgc ttcatatttc
     8581 ttttgtttcc ctttgtaatt tattttttaa taaaatctat aaaaaataga cagggaaaat
     8641 gatttgttta gatataaaac gttgacaaaa gcaaaattaa gcgtttatca tttatcttta
     8701 gtaattagat tagcgagggg gaaatgacat tggcagataa tttagtcatt gttgaatcgc
     8761 ctgcaaaagc aaaaaccatt gaaaagtatt taggtaagaa atataaagtt atagcttcaa
     8821 tgggacacgt cagagactta ccaagaagtc aaatgggtgt cgacactgaa gataattacg
     8881 aaccaaaata tataacaata cgcggaaaag gtcctgttgt aaaagaattg aaaaaacatg
     8941 caaaaaaagc gaaaaacgtc tttctcgcaa gtgaccccga ccgtgaaggt gaagcaattg
     9001 cttggcattt atcaaaaatt ttagagcttg aagattctaa agaaaatcgc gttgttttca
     9061 acgaaataac taaagacgct gttaaagaaa gttttaaaaa tcctagagaa attgaaatga
     9121 acttagtcga tgcacaacaa gcgcgtcgaa tattagatag attagttggc tataacatct
     9181 cgccagttct ttggaaaaaa gtaaaaaaag ggttgtcagc gggtcgagtt caatctgttg
     9241 cacttcgttt agtcattgac cgtgaaaatg aaattcgaaa ctttaaacca gaagaatatt
     9301 ggactattga aggagaattt agatacaaaa aatcaaaatt caatgctaaa ttccttcatt
     9361 ataaaaataa accttttaaa ctaaaaacga aaaaagatgt tgagaaaatt acagctgcat
     9421 tagatggaga tcaattcgaa attacaaacg tgactaaaaa agaaaaaacg cgtaatccag
     9481 caaacccatt tacaacttct acattacaac aagaggcggc acgtaaatta aactttaaag
     9541 caagaaaaac aatgatggtc gcacaacaat tatatgaagg tatagatttg aaaaaacaag
     9601 gtacgattgg tttaataaca tatatgagaa ccgattctac acgtatttca gatactgcca
     9661 aagttgaagc aaaacagtat ataactgata aatacggtga atcttacact tctaaacgta
     9721 aagcatcagg gaaacaaggt gaccaagatg cccatgaggc tattagacct tcaagtacta
     9781 tgcgtacgcc agatgatatg aagtcatttt tgacgaaaga ccaataccga ttatacaaat
     9841 taatttggga acgatttgtt gctagtcaaa tggctccagc aatacttgat acagtctcat
     9901 tagacataac acaaggtgac attaaattta gagcgaatgg tcaaacaatc aagtttaaag
     9961 gatttatgac actttatgta gaaactaaag atgatagtga tagcgaaaag gaaaataaac
    10021 tgcctaaatt agagcaaggt gataaagtca cagcaactca aattgaacca gctcaacact
    10081 atacacaacc acctccaaga tatactgagg cgagattagt aaaaacacta gaagaattga
    10141 aaattgggcg accatcaact tatgcaccga caatagatac gattcaaaag cgtaactatg
    10201 tcaaattaga aagtaagcgt tttgttccta ctgagttggg agaaatagtt catgaacaag
    10261 tgaaagaata cttcccagag attattgatg tggaattcac agtgaatatg gaaacgttac
    10321 ttgataagat tgcagaaggc gacattacat ggaggaaagt aatcgacggt ttctttagta
    10381 gctttaaaca agatgttgaa cgtgctgaag aagagatgga aaagattgaa atcaaagatg
    10441 agccagccgg tgaagactgt gaaatttgtg gttctcctat ggttataaaa atgggacgct
    10501 atggtaagtt catggcttgc tcaaacttcc cggattgtcg taatacaaaa gcgatagtta
    10561 agtctattgg tgttaaatgt ccaaaatgta atgatggtga cgtcgtagaa agaaaatcta
    10621 aaaagaatcg tgtcttttat ggatgttcga aatatcctga atgcgacttt atctcttggg
    10681 ataagccgat tggaagagat tgtccaaaat gtaaccaata tcttgttgaa aataaaaaag
    10741 gcaagacaac acaagtaata tgttcaaatt gcgattataa agaggcagcg cagaaataat
    10801 atttttattt cctagataca ttttaagatt gttaaataga atcattagtg aatcttattt
    10861 taaagatagt aaaggattaa tctaaataag tgcggataat ataaacataa caacataatt
    10921 aaaagacata aatgacaata aaaggagtat agaaatgact caaactgtaa atgtaatagg
    10981 tgctggtctt gccggttcag aagcggcata tcaattagct gaaagaggaa ttaaagttaa
    11041 tctaatagag atgagacctg ttaaacaaac accagcgcac catactgata aatttgcgga
    11101 acttgtatgt tccaattcat tacgcggaaa tgctttaact aatggtgtgg gtgttttaaa
    11161 agaagaaatg agaagattga attctataat tattgaagcg gctgataagg cacgagttcc
    11221 agctggtggt gcattagcag ttgatagaca cgatttttca ggttatatta ctgaaacact
    11281 taaaaatcat gaaaatatca cagttattaa tgaagaaatt aatgccattc cagatggata
    11341 cacaattatc gcaacaggac cacttactac agaaaccctt gcgcaagaaa tagtggacat
    11401 tactggtaaa gatcaacttt atttctatga tgcggctgct ccaattattg aaaaagaatc
    11461 tattgatatg gataaagttt acttaaagtc ccgttatgat aaaggtgaag ctgcatattt
    11521 aaactgtcct atgactgagg atgaatttaa tcgcttttat gatgcagtat tagaagctga
    11581 agttgcgcct gtaaattcat ttgaaaaaga aaaatatttc gagggttgta tgccttttga
    11641 agtaatggca gaacgcggac gcaagacatt actatttgga ccaatgaaac cagtaggatt
    11701 agaagatcca aagactggga aacgtcctta tgcggtggtt caattaagac aagatgacgc
    11761 tgctggtaca ctctacaata ttgttggctt ccaaacgcat ttaaaatggg gagctcaaaa
    11821 agaagtcatt aaattaattc caggcttaga aaatgttgat attgttagat atggtgtgat
    11881 gcatagaaat accttcatta attcaccgga cgtattaaac gagaaatatg aattgatttc
    11941 acaaccaaac atacagtttg cgggacaaat gactggtgtt gaaggttatg tagaaagcgc
    12001 agctagcggc ttagttgcag gtatcaatct tgcgcataaa atattaggca agggtgaggt
    12061 agtatttccg agagaaacaa tgattggaag tatggcttac tatatttctc atgctaaaaa
    12121 caataagaat ttccaaccta tgaatgctaa cttcgggtta ttaccatctt tagaaactag
    12181 aattaaagat aaaaaagaac gctatgaagc acaagctaat agagctttgg attacttaga
    12241 aaatttcaaa aaaactttat aaaatagtta gaaagactag atatgctatt cattcttaag
    12301 tcatcaacga gtaagtaatg actttctaaa tggaaaatac ttatcctagt ctttttaatt
    12361 ttggaattgt tacgtatttc tgacaattta gaattcgcat tcaaaaaata tctaaataaa
    12421 taacacgcaa taagttgatt gatgtaacat gtaagagaat gttttaaata aactttattt
    12481 aaaaggcaat gaaataataa atggcaaggc tattaataaa gacttttagt aattaattta
    12541 aaaaagaggt attctaatta acaggttttc cgattagtta caattattta attctcaaaa
    12601 gatttagaat tgattatcaa attactgtaa gccctttgct gtatatgcta caattcttat
    12661 tgatggaggg taaatgtatt gaatcatatt caagatgcgt ttttaaatac attgaaagtt
    12721 gaacggaatt tttcggaaca cacattgaaa tcatatcaag atgacttaat tcagtttaat
    12781 caatttttag aacaagaaca tttagagttg aatacttttg aatacagaga tgctagaaat
    12841 tatttgagct atttatattc aaatcatttg aaaagaacat ctgtttctcg taaaatctca
    12901 acgttaagaa ctttctatga atattggatg acgcttgatg agaacattat taatccattt
    12961 gttcaattag tacatccgaa aaaagaaaaa tatcttccgc aattctttta cgaagaagaa
    13021 atggaagcgt tattcaaaac tgtagaagag gacacttcaa aaaatttacg ggatcgagtt
    13081 attcttgaat tgttgtatgc tacaggcatc cgtgtttcgg aattagtaaa tattaaaaaa
    13141 caagatatag atttttacgc gaatggtgtt accgtattag gaaaagggag caaagagcgc
    13201 tttgtaccgt ttggtgctta ttgtagacaa agcatcgaaa attatttaga acatttcaaa
    13261 ccaattcagt catgcaatca tgattttctt attgtaaata tgaagggtga agcaatcact
    13321 gaacgcggtg tacgatatgt tttaaatgat attgttaaac gaacagcagg cgtaagtgag
    13381 attcatcccc acaagctcag acatacattt gcaacgcatt tattgaatca aggtgcagac
    13441 ctaagaacag tacaatcgtt attaggtcat gttaatttgt caacaactgg taaatataca
    13501 cacgtatcta accaacaatt aagaaaagtg tatctaaatg cacatcctcg agcgaaaaag
    13561 gagaatgaaa catgagtaat acaacattac atgcaacaac aatttatgct gtaagacata
    13621 atgggaaagc agctatggct ggagatgggc aagtaacgct tggtcaacaa gtcatcatga
    13681 aacaaacggc aagaaaagtg cgacgtttat atgaaggtaa agtgttagct ggtttcgcag
    13741 gtagtgtagc agatgcgttt acgttatttg aaaaattcga aacaaaatta caacagttta
    13801 gtggtaactt agaaagagct gctgttgaat tggcacaaga atggcgaggc gataaacaat
    13861 tacgtcaatt agaagctatg ctaattgtaa tggataaaga tgctatttta gttgtcagtg
    13921 gaactggcga agttattgct ccagatgatg accttatcgc tattggatca ggaggcaact
    13981 acgcattaag cgcaggacgt gcattgaaac gccatgcatc gcatttgtct gctgaagaaa
    14041 tggcatatga gagcttgaaa gtagcggctg atatttgtgt ctttaccaac gataatattg
    14101 ttgtcgaaac actataataa tcagagcacg ataaataatt acgagcaatt aattttagtt
    14161 aaaagacgga ggaatgaaat taatggatac agctggaata agattaactc caaaagaaat
    14221 cgtatctaaa ttaaatgaat acatcgttgg acaaaatgat gctaaacgta aagtggcaat
    14281 tgccctacgt aatcgataca gaagaagttt attagatgag gaatcaaagc aagaaatttc
    14341 acctaaaaat attttgatga ttggaccaac cggcgttggt aaaactgaaa ttgcaagaag
    14401 aatggccaaa gttgtcggcg cgccatttat aaaagtagaa gctactaaat ttactgaggt
    14461 aggttatgta ggacgagatg ttgaaagtat ggttagagat cttgttgatg tttcagtaag
    14521 attagtcaag gcgcagaaaa aatcattggt acaagatgaa gcaacagcta aggccaatga
    14581 aaaacttgtt aagttattag ttccaagtat gaaaaagaaa gcgtctcaaa cgaataatcc
    14641 tttagagtca cttttcggag gtgcaattcc aaatttcgga caaaataacg aagatgaaga
    14701 agaaccacct actgaggaaa ttaaaacaaa acgttctgaa attaagagac agctagaaga
    14761 aggcaaactt gaaaaagaaa aggtaagaat taaagtcgaa caagatcctg gtgctttagg
    14821 tatgctaggt acaaatcaaa atcagcaaat gcaagagatg atgaatcaat taatgcctaa
    14881 aaagaaagtt gagcgagaag ttgctgttga gacggcaagg aaaatcttag ctgatagtta
    14941 tgcggatgaa ctaattgatc aagaaagcgc taaccaagaa gcgcttgaat tagcagaaca
    15001 aatgggtatc atctttatag atgaaatcga caaagttgcg acgaataatc ataatagtgg
    15061 tcaagatgtc tcaagacaag gtgttcaaag agatatttta cctatacttg aaggtagcgt
    15121 tattcaaacc aaatatggta ctgtgaatac tgaacatatg ctgtttatag gtgctggagc
    15181 tttccatgta tctaagccga gtgacttgat accagaattg caaggtcgtt ttccgattag
    15241 agttgaactt gatagtttat cggtagaaga ttttgtaaga attttgacag aaccaaaatt
    15301 gtcattaatt aaacaatatg aagcattgct tcaaacagaa gaagttactg taaactttac
    15361 cgatgaagca attactcgct tagctgagat tgcttatcaa gtaaatcaag atacagacaa
    15421 cattggtgca cgtcgacttc atacaatttt agaaaagatg ctagaagatt tatcattcga
    15481 agcaccaagt atgccgaatg cagttgtaga tattacccca caatatgttg atgataaatt
    15541 aaaatcaatt tcaacaaata aagatttaag tgcatttatt ctataaaaat atacaaaagg
    15601 agaaaaattc atgagcttat tatctaaaac gagagagtta aacacgttac ttcaaaaaca
    15661 caaaggtatt gcggttgatt ttaaagatgt agcacaaacg attagtagcg taactgtaac
    15721 aaatgtattt attgtatcgc gtcgaggtaa aattttagga tcgagtctaa atgaattatt
    15781 aaaaagtcaa agaattattc aaatgttgga agaaagacat attccaagtg aatatacaga
    15841 acgattaatg gaagttaaac aaacagaatc aaatattgat atcgacaatg tattaacagt
    15901 attcccacct gaaaacagag aattattcat agatagtcgt acaactatct tcccaatttt
    15961 aggtggaggg gaaagattag gtacattagt acttggtcga gtacatgatg attttaatga
    16021 aaatgatttg gtactaggtg aatatgctgc tacagttatt ggtatggaaa tcttacgtga
    16081 gaagcatagt gaagtagaaa aagaagcgcg cgataaagct gctattacaa tggcaattaa
    16141 ttcattatct tattctgaaa aagaagcgat tgaacatatc tttgaagaac ttggcggtac
    16201 ggaaggccta ttaatcgcat caaaagttgc agatagagtt ggtattacta gatctgtaat
    16261 tgtaaatgca ctacgtaaat tagaaagtgc tggtgtaatt gaatcacgtt ctttaggaat
    16321 gaaaggtact ttcattaaag ttaaaaaaga aaaattctta gatgaattag aaaaaagtaa
    16381 ataagtcgat gagtctggga cataattcaa tgtcctagtc gtgacgatgt tatcttggcg
    16441 gtagtcaatg aaatgacttt gatgattatg ttgaagtcat ttctttattt ttgtctaatt
    16501 ttcaatcaaa ttgatatttc acattaactt gatagaatta cctttaaatt tatggaaaaa
    16561 tgcttgaaaa acatttgctt tagacacggc attatgttat atttattttc ggctataaag
    16621 ccaatacaca catatcaaac gtgattgtgt aaagggtgca atatttatat attgtcttta
    16681 caatagtttg ctatggaggt aattaaccaa taggaggaat ttataatggc agtaatttca
    16741 atgaaacaat tactagaagc gggtgttcac ttcggtcacc aaacacgtcg ttggaaccca
    16801 aaaatgaaaa aatatatctt cactgagaga aatggtattt atatcatcga cttacaaaaa
    16861 acagtgaaaa aagtagacga ggcatacaac ttcttgaaac aagtttcaga agatggtgga
    16921 caagtcttat tcgtaggaac taaaaaacaa gcacaagaat cagttaaatc tgaagcagaa
    16981 cgtgctggtc aattctacat taaccaaaga tggttaggtg gattattaac taactataaa
    17041 acgatctcaa aacgaatcaa acgtatttct gaaattgaaa aaatggaaga agatggttta
    17101 ttcgaagtat tacctaaaaa agaagtagta gaacttaaaa aagaatacga ccgtttaatc
    17161 aaattcttag gcggaattcg tgatatgaaa tcaatgcctc aagcattatt cgtagttgac
    17221 ccacgtaaag agcgtaatgc aattgctgaa gctcgtaaat taaatattcc tatcgtaggt
    17281 atcgttgaca ctaactgtga tcctgacgaa attgactacg ttatcccagc aaacgacgat
    17341 gctatccgtg cggttaaatt attaactgct aaaatggcag atgcaatctt agaaggtcaa
    17401 caaggcgttt ctaatgaaga agtagctgca gaacaaaaca tcgatttaga tgaaaaagaa
    17461 aaatcagaag aaacagaagc aactgaagaa taatcaactg ttgaatctga cttagatata
    17521 gtttaaatgg gtgataagat attaatgctt atcacctttt ttaaaaagaa aatcgaggca
    17581 aattacaaat attcaattag agtattggca atcttgccta taataatgct aaaatcataa
    17641 tatataaaat gataacttat tggaggaata atgaatggca actatttcag caaaacttgt
    17701 taaagaatta cgtgaaaaaa ctggcgcggg tatgatggat tgtaaaaaag cgctaactga
    17761 aactgatggt gacatcgata aagcgattga ctacctacgt gaaaaaggta ttgctaaagc
    17821 agctaaaaaa gcagaccgta ttgcggctga aggtttagta catgtagaaa ctaaaggtaa
    17881 cgacgcagtt atcgttgaaa tcaactctga aacagacttt gttgctcgta acgaaggttt
    17941 ccaagagtta gttaaagaaa tcgctaatca agtattagat acaaaagctg aaactgttga
    18001 agctttaatg gaaacaactt taccaaatgg taaatcagtt gatgaaagaa ttaaagaagc
    18061 aatttcaaca atcggtgaaa aattaagtgt tcgtcgtttt gctatcagaa ctaaaactga
    18121 taacgatgct ttcggcgctt acttacacat gggtggacgc attggtgtat taacagttgt
    18181 tgaaggttca actgacgaag aagcagcaag agacgttgct atgcatatcg ctgcaatcaa
    18241 ccctaaatat gtttcttctg aacaagttag cgaagaagaa atcaaccacg aaagagaagt
    18301 tttaaaacaa caagcattaa atgaaggtaa accagaaaac atcgttgaaa aaatggtgga
    18361 aggacgttta cgtaaatact tacaagaaat ttgtgctgta gatcaagact tcgttaaaaa
    18421 ccctgatgta acagttgaag ctttcttaaa aacaaaaggt ggaaaacttg ttgacttcgt
    18481 acgctatgaa gtaggcgaag gtatggaaaa acgcgaagaa aactttgcgg atgaagttaa
    18541 aggacaaatg aaataatctg tcataaagta aaacaaggaa gaagacacct ttaatgttgc
    18601 tttattaaaa tgtaaatcat tctaataaaa cgacaactgt gtcttcttta cttgtatatg
    18661 ttacatatat tcacgataga gaggataaga aaatggctca aatttctaaa tataaacgtg
    18721 tagttttgaa actaagtggt gaagcgttag ctggagaaaa aggatttggc ataaatccag
    18781 taattattaa aagtgttgct gagcaagtgg ctgaagttgc taaaatggac tgtgaaatcg
    18841 cagtaatcgt tggtggcgga aacatttgga gaggtaaaac aggtagtgac ttaggtatgg
    18901 accgtggaac tgctgattac atgggtatgc ttgcaactgt aatgaatgcc ttagcattac
    18961 aagatagttt agaacaattg gattgtgata cacgagtatt aacatctatt gaaatgaagc
    19021 aagtggctga accttatatt cgtcgtcgtg caattagaca cttagaaaag aaacgcgtag
    19081 ttatttttgc tgcaggtatt ggaaacccat acttctctac agatactaca gcggcattac
    19141 gtgctgcaga agttgaagca gatgttattt taatgggcaa aaataatgta gatggtgtat
    19201 attctgcaga tcctaaagta aacaaagatg cggtaaaata tgaacattta acgcatattc
    19261 aaatgcttca agaaggttta caagtaatgg attcaacagc atcctcattc tgtatggata
    19321 ataacattcc gttaactgtt ttctctatta tggaagaagg aaatattaaa cgtgctgtta
    19381 tgggtgaaaa gataggtacg ttaattacaa aataaattta gaggtgtaaa ataatgagtg
    19441 acattattaa tgaaactaaa tcaagaatgc aaaaatcaat cgaaagctta tcacgtgaat
    19501 tagctaacat cagtgcagga agagctaatt caaatttatt aaacggcgta acagttgatt
    19561 actatggtgc accaacacct gtacaacaat tagcaagcat caatgttcca gaagcacgtt
    19621 tacttgttat ttctccatac gacaaaactt ctgtagctga catcgaaaaa gcgataatag
    19681 cagctaactt aggtgttaac ccaacaagtg atggtgaagt gatacgtatt gctgtacctg
    19741 ccttaacaga agaacgtaga aaagagcgcg ttaaagatgt taagaaaatt ggtgaagaag
    19801 ctaaagtatc tgttcgaaat attcgtcgtg atatgaatga tcagttgaaa aaagatgaaa
    19861 aaaatggcga cattactgaa gatgagttga gaagtggtac tgaagatgtt cagaaagcaa
    19921 cagacaattc aataaaagaa attgatcaaa tgattgctga taaagaaaaa gatattatgt
    19981 cagtataaaa ctaatataca atgacatatt aaaatgccag tattaaacga taatgtaaca
    20041 tttaaaatgg gcatgtttaa ttaaatcaaa gatgcatgtg ataatttaaa ttcacaatga
    20101 gcataaaaat ggtgtttaaa caagttaatt aaacatatac tttataaata ataggcatta
    20161 ggtatattgc tataataaag ttatgtaatt tttaacctca gtatgtatgt cacatttctg
    20221 gtgtaaactg taccgagtca gactttggta cagttttttt atttgcttat tcaatgcatt
    20281 aaatgagtat gataaaatga taatgattgt ttagtaactt atactatatg acagagatga
    20341 tcaggctcgg aggaaagacc atgtttaaaa agctaataaa taaaaagaac actataaata
    20401 attataatga agaattagac tcgtctaata tacctgaaca tatcgctatt attatggatg
    20461 gtaatgggcg atgggctaag aagcgaaaaa tgcctagaat taaaggtcat tacgaaggta
    20521 tgcaaacaat aaaaaaaatt actagggtag ctagtgatat tggtgttaag tacttaactt
    20581 tatacgcctt ttccactgaa aattggtcaa gacctgaaag tgaagtaaat tatattatga
    20641 atttgcctgt caatttctta aagacattct taccggaact aattgaaaaa aatgtcaaag
    20701 ttgaaacaat tggatttact gataagttgc caaaatcaac gatagaagca attaataatg
    20761 ctaaagaaaa gacagctaat aataccggct taaaattaat atttgcaatt aattatggtg
    20821 gcagagcaga acttgttcat agtattaaaa atatgtttga cgagcttcat caacaaggtt
    20881 taaatagtga tatcatagat gaaacatata taaacaatca tttaatgaca aaagactatc
    20941 ctgatccaga gttgttaatt cgtacttcag gagaacaaag aataagtaat ttcttgattt
    21001 ggcaagtttc gtatagtgaa tttatcttta atcaaaaatt atggcctgac tttgacgaag
    21061 atgaattaat taaatgtata aaaatttatc agtcacgtca aagacgcttt ggcggattga
    21121 gtgaggagta gtatagtatg aaagttagaa cgctgacagc tattattgcc ttaatcgtat
    21181 tcttgcctat cttgttaaaa ggcggccttg tgttaatgat atttgctaat atattagcat
    21241 tgattgcatt aaaagaattg ttgaatatga atatgattaa atttgtttca gttcctggtt
    21301 taattagtgc agttggtctt atcatcatta tgttgccaca acatgcaggg ccatgggtac
    21361 aagtaattca attaaaaagt ttaattgcaa tgagctttat tgtattaagt tatactgtct
    21421 tatctaaaaa cagatttagt tttatggatg ctgcattttg cttaatgtct gtggcttatg
    21481 taggcattgg ttttatgttc ttttatgaaa cgagatcaga aggattacat tacatattat
    21541 atgccttttt aattgtttgg cttacagata caggggctta cttgtttggt aaaatgatgg
    21601 gtaaacataa gctttggcca gtaataagtc cgaataaaac aatcgaagga ttcataggtg
    21661 gcttgttctg tagtttgata gtaccacttg caatgttata ttttgtagat ttcaatatga
    21721 atgtatggat attacttgga gtgacattga ttttaagttt atttggtcaa ttaggtgatt
    21781 tagtggaatc aggatttaag cgtcatttcg gcgttaaaga ctcaggtcga atactacctg
    21841 gacacggtgg tattttagac cgatttgaca gctttatgtt tgtgttacca ttattaaata
    21901 ttttattaat acaatcttaa tgctgagaac aaatcaataa acgtaaagag gagttgctga
    21961 gataatttaa tgaatctcag aactcc
//
LOCUS       DANRDI010000031        21506 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.031, whole genome shotgun sequence.
ACCESSION   DANRDI010000031 DANRDI010000000
VERSION     DANRDI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.031"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            198..902
                     /locus_tag="PZ377_000907"
     CDS             198..902
                     /locus_tag="PZ377_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDM6357793.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1295..1831
                     /locus_tag="PZ377_000908"
     CDS             1295..1831
                     /locus_tag="PZ377_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357794.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(1944..3485)
                     /gene="guaA"
                     /locus_tag="PZ377_000909"
     CDS             complement(1944..3485)
                     /gene="guaA"
                     /locus_tag="PZ377_000909"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDM6357795.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3510..4976)
                     /gene="guaB"
                     /locus_tag="PZ377_000910"
     CDS             complement(3510..4976)
                     /gene="guaB"
                     /locus_tag="PZ377_000910"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDM6357796.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5014..6282)
                     /locus_tag="PZ377_000911"
     CDS             complement(5014..6282)
                     /locus_tag="PZ377_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDM6357797.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6282..6860)
                     /gene="xpt"
                     /locus_tag="PZ377_000912"
     CDS             complement(6282..6860)
                     /gene="xpt"
                     /locus_tag="PZ377_000912"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDM6357798.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7091..7193)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7373..7780
                     /locus_tag="PZ377_000913"
     CDS             7373..7780
                     /locus_tag="PZ377_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDM6357799.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            7923..8585
                     /locus_tag="PZ377_000914"
     CDS             7923..8585
                     /locus_tag="PZ377_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357800.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8703..9659
                     /locus_tag="PZ377_000915"
     CDS             8703..9659
                     /locus_tag="PZ377_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357801.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(10450..10551)
                     /locus_tag="PZ377_000916"
     CDS             complement(10450..10551)
                     /locus_tag="PZ377_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357802.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            10636..12024
                     /locus_tag="PZ377_000917"
     CDS             10636..12024
                     /locus_tag="PZ377_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDM6357803.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(12105..12860)
                     /locus_tag="PZ377_000918"
     CDS             complement(12105..12860)
                     /locus_tag="PZ377_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HDM6357804.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            13352..13921
                     /gene="ahpC"
                     /locus_tag="PZ377_000919"
     CDS             13352..13921
                     /gene="ahpC"
                     /locus_tag="PZ377_000919"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDM6357805.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            13937..15460
                     /gene="ahpF"
                     /locus_tag="PZ377_000920"
     CDS             13937..15460
                     /gene="ahpF"
                     /locus_tag="PZ377_000920"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDM6357806.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            15590..15832
                     /locus_tag="PZ377_000921"
                     /pseudo
     CDS             15590..15832
                     /locus_tag="PZ377_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15905..16531
                     /locus_tag="PZ377_000922"
     CDS             15905..16531
                     /locus_tag="PZ377_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDM6357807.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            16786..17169
                     /locus_tag="PZ377_000923"
     CDS             16786..17169
                     /locus_tag="PZ377_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357808.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(17235..17816)
                     /locus_tag="PZ377_000924"
     CDS             complement(17235..17816)
                     /locus_tag="PZ377_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDM6357809.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            17994..18203
                     /locus_tag="PZ377_000925"
     CDS             17994..18203
                     /locus_tag="PZ377_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357810.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(18242..18493)
                     /locus_tag="PZ377_000926"
     CDS             complement(18242..18493)
                     /locus_tag="PZ377_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDM6357811.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            18793..19056
                     /locus_tag="PZ377_000927"
     CDS             18793..19056
                     /locus_tag="PZ377_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6357812.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(19194..19766)
                     /locus_tag="PZ377_000928"
     CDS             complement(19194..19766)
                     /locus_tag="PZ377_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDM6357813.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(19947..20315)
                     /locus_tag="PZ377_000929"
     CDS             complement(19947..20315)
                     /locus_tag="PZ377_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDM6357814.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(20684..21295)
                     /locus_tag="PZ377_000930"
     CDS             complement(20684..21295)
                     /locus_tag="PZ377_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDM6357815.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
BASE COUNT         7226 a         3898 c         3257 g         7125 t
ORIGIN      
        1 ttttacacat acatgaacta taaaaagcga ataaatagca ggtcattgcg aaatttgcta
       61 aatacaaaat tgatgagttc cacctaataa aaattgttgg ttttatagta acatctattt
      121 tcgtaccatt catgttaatt tcagtgctaa taatgctgat ataaagaaga ataaattgat
      181 taggagaaca ctatttaatg tctaaaaata tcaatcaagc aaatagtaaa ttaaatacgt
      241 caaacaaaaa actaaaacaa gcttattcaa aatccgacag taaaaacatg aaagttatat
      301 acatgcctca ttggctagag ttttattcaa ttgctataca tagcgatgtt acaagcaata
      361 aaaaaagata ttataaatca tatagtagag gaacagtggt atatgtaaaa ctagggagta
      421 atattggaag tgagttttct ggtaatcatt tttgtgtaat attagataac aaagacaata
      481 aaggaaaaga aacagttaca atagttccac tttcttccaa aggtaataaa aattatttga
      541 agttaaacga atcggtgcta aatctaacag caacagattt aaaaaaacag attatcgata
      601 tcagtagtaa agtacaagca ttagcccaaa aatatagaga aattgacatt aaacttagca
      661 cagaagataa aaagcttttg tctaacttaa atcaaaaagc taatgaactg tatagagtga
      721 taggtgttta ttccaagcac aaagggaaag atacatacgt taatatatca gcaattacaa
      781 caataagcaa aagacgtata agcaaaataa atgattctga tcctactgga acgataataa
      841 tttcagaaga tgacatgaat gaaatcgaaa agcaattaaa gataaagttc ttttctaatt
      901 gaattgtaat attacatctg atataattga tttacaaatt cccggtaacc attccggctt
      961 cattctcttg aagaatgaca tattctcagc gttttagctg aaggtcagat gatacgtcat
     1021 ctggcctctt tttttatata aatgcccttt aagtgaaata tacaacaatc tgcaatcaat
     1081 attagacatc acaaaacaca caataaatta cacaatgtgt tataagccac atgtcactat
     1141 tatcgcagct agctgcgtca ctcaaaacga atacggaacg cgttggttat ttcaatcata
     1201 atattactct gcaaatacac agccctgtaa ccgagtaaat ggatagagat ttgaacaaat
     1261 gaaaacaatc aactaatgga aaggataaaa tattatgcac aacaataatg aattcaacaa
     1321 aaaactgaaa gattttatag gtagcgataa acggatggct ttagtaaagg gttatgttaa
     1381 cgagtataaa ttaacaactg ttttaagagc tttaaataat tccgattaca caaaagggac
     1441 gatacacact cgttcaatag gcaacctaaa acaaatagta gataatcgaa ttattcctaa
     1501 aaatataact caaaacacaa tcttcaaatt gagtaattta accttagagg ttaacctcta
     1561 tgaaagaaac acaatttatc acggagactt tgctatttac tatcctgtag aatctgctct
     1621 aataatagaa acagatactc ctaggctaat taatcatata aataacaacc cagtccaaaa
     1681 aatatttata ataacaacaa atgactggag ctttaataca cttgaattag aaaaaatagt
     1741 agacgaaact ataatttatg atttaaaaca agaagatcct aaacaatatg aaattttata
     1801 taaaaataaa cacggaaaat tgccttattg atgaattaaa acgcaaactt agaacacctt
     1861 accccttttc cgtccttatt gtaattttcc aactactcaa attaaaaacc acccgtgcaa
     1921 acaggtggtt ttattatata taattattcc cactcaattg tgcttggtgg ttttgatgta
     1981 atgtcataga ctacgcggtt gacgtgatct acttcgttta cgatacgact agaaatcttt
     2041 tgtaagactt cccaatcgat gcgtgcgaag tcacttgtca taccgtcgat agatgttact
     2101 gcacgaatac ctactgtgtg atcatacgta cggtagtctc ccataacacc tactgattga
     2161 atgtttggta acactgtgaa gtattgccaa atttctcttt caagaccttc ttctctaatc
     2221 acttggcgta aaatcgcgtc tgattctcta acgatttcta gtttatcttc agtaatttct
     2281 ccaagtacac gaataccaag accaggtcct gggaatggtt gtctccatac taaatgttct
     2341 ggaataccta actcaatacc taatttacgt acttcatctt taaacaatgt attgattggt
     2401 tcgattaatt cgaattccat gtcttctggt aatccaccaa cattgtggtg tgatttgatt
     2461 gtttgtgctg ttttagtacc tgattcgatg acgtctgtat atagtgttcc ttgcgcaagg
     2521 aagtctacac ctttcagttt tgatgcttca tcatcaaata cgtatacaaa ttcattacca
     2581 atgattttac gtttttgttc aggatctgaa acacctttta atttattcat aaagcgatct
     2641 ttcgcattaa cacgaataat attcatgttg aaaccttcac cgaattgctc cataaccatg
     2701 tcgccttcac ctttacgaag taagccatgg tctacaaaga tacatgttag ttgatcacct
     2761 attgctttat gcaatagtac agctacaaca gatgaatcta cgccgccact catcgcacat
     2821 aatacacgac ggtctcctac gcgttgacga atcttttcaa tttcgatttc gataaagttt
     2881 tccattgtcc attgacctct acaatcacaa acacgacgga caaaattatt taataaatca
     2941 ttaccatatt ctgtatgacg tacttctgga tggaattgaa caccataaat gcgacgtttc
     3001 ttatcttcga ttgctgcata gtctgtgctt gggctatcag cgataacttc aaagccttct
     3061 ggaatttcaa taactttatc agaatgactc atccaaacag tttgttctgc tggtaagcca
     3121 gcgaataact catctgactt cgcattaatg attgctttac cgtattcacg ttcattggca
     3181 cgttcaactt taccacctaa taatttagta gttaattgca tgccgtaaca aataccaagt
     3241 actggaattc ctaaattata tatttccgga tcaattgtaa atgaaccttc ttcataaact
     3301 gaatttggac cacctgataa gataatacct tttggattca ttttcttaat ttcttcaatt
     3361 gaaatttcat gatcgtgtaa ttcactataa acgcccattt cacgaattcg gcgtgtaatt
     3421 aattggttgt attggctacc aaagtctaag acaaggatta actcttgttc ttttgccatt
     3481 tccatatttg tcgttctcct ttatcttaat tagaatgagt agttcggtga ttctttcgta
     3541 atttgaatat tatgtggatg gctttctgct aaaccagcag gacccatacg tgtaaattgt
     3601 gcttcttcgc gtaattctct taaatcgtgt gaaccagtat aacccatacc agcacgcaca
     3661 ccgcccatta attggtaaat tgtatcttgt aacgcacctt tataagccgt acgtccttcg
     3721 ataccttcag gaacaaattt cttaggcgct ttgtcctctt ggaagtaacg gtcgtttgaa
     3781 cctttttcca tcgcacctaa agagcccata ccacggtata ctttatattg tctaccttgg
     3841 aaaatttctg ttgcgcctgg gctttcttca gtacctgcta ataagctacc taacataacc
     3901 gcatgtccac cagcagctaa tgctttaatg atatctcctg agaatttaat accaccatca
     3961 gcaatgatag ctttaccatg tttgcgtgct tcagttgcac aatcataaat tgctgtaatt
     4021 tgtggtacac caacacctgc tacaacacgc gtcgtacaaa ttgaacctgg gccaatacca
     4081 actttaacaa tatctgcacc cgcttcaaat aaatcttttg ttgcttctgc agttgctacg
     4141 ttacctgcta ctaatgtgat ttctgggtaa gtcttcttaa tatgtttcac ttgatcgata
     4201 acacctttag agtgaccatg tgctgtatcg ataactaaga catccacacc tgcttcgact
     4261 aatttttgag cacgaatatc agtatctttt gaaataccaa ttgctgcggc tacaagtaga
     4321 cgaccatgtt catcttttgc tgcattaggg aattcgataa ctttttcaat atctttaata
     4381 gtaataagac cttctagacg tccgtcttta actaatggta acttttcaat cttatgtttt
     4441 tggagaattt tttctgcttc ttcaagtgtt gtattcactg gagctgtaat taaattttct
     4501 tgcgtcatta catctacaat tttaatcgag aagtcttcaa taaaacgtaa gtcacggttt
     4561 gttaaaatac ctactaagtt gcgatcttct ttattatcaa caattggtac acctgaaata
     4621 cggtatttac ccattaatgc ttctgcttca taaacgcttt cttctggcgt taagaaaaat
     4681 gggtttgaaa tgacaccatt ttctgagcgt tttacttttt gaacttcgtc cgcttgttct
     4741 tcaacgccca tatttttatg aataacacct aaaccacctt gacgagccat agcaatcgcc
     4801 attttagatt cagttacagt atccatacca gcagaaataa ctggaatatt taatttaact
     4861 ttgtctgata attgtacgct taaatcaacg tctttcggta aaatatcaga ttgtgctgga
     4921 attaataaca catcatcaaa cgttaatgat tcttttgcaa atttactttc ccacattaaa
     4981 aacagcctcc atttttcaaa ttaattagtt atattatttc acattttctt ccgtttgttt
     5041 atactttatt ccattaaaaa agaaattaag aataattgca gagattgcgc ctaaaacaat
     5101 tccgttttga gtcaaccatg caaattgttc acctaaacct ttaaatgctt gtggtacagc
     5161 gcttatacca gtacctaatc ctactgatac agcgataatt aataaattgt tttgattttt
     5221 aaaatcgata tgtcctaata tactaacacc atatgccatt accatgccaa acatagctat
     5281 catcgcaccg cctaacacag gtagcggtat gatatttgct aatgcgccaa gcttaggtat
     5341 acaaccacat ataagtaata acacgaccat gccgtatata acattgtttt tcttagcgcc
     5401 ggataaagaa acaagtccta cattttgcga ataggctgta tacggaaatg aattgaatat
     5461 agaacctaac actatcgcta gaccttccgc agtataacct ttacgaaaat cttttctttc
     5521 taacttctta ccggtaattt cacttaacgc atgatagaca cctgtcgact caattaaact
     5581 aacgatagct acaataaaga acactaacgt cgatgtcaca tcaaagctaa atccagagaa
     5641 tctaaacggc actgggatgc ctaaccaacc ggcatgattg acttgattaa tatcgaccat
     5701 cccaagtaag ccagcaccta tcgttcctaa aacgagtcca attaatatgg caatactctt
     5761 aataaatcca gttgtgaatc tttgtaaaag aagaataatg attaatgtca ttaaacctaa
     5821 caaaatgttc ttaacatctc catagtcctt tgcaccttga cctccagcta agtaattcat
     5881 tgctactggc attaaattga taccaatgat agtaacaaca ctacccgtta ctactggtgg
     5941 gaagaatttt acaagatgtg aaaagaaagg cgcgatgata ataactaata tccctgataa
     6001 aaatagcgaa ccataaagta catctattcc tttcgtttga ccaattaaaa tcatgggcgc
     6061 aacagccgtg aatgtacatc caagaacgat tggtaatcct gttcctgtta ctttattggc
     6121 ttgtaaaaat gtggcaaccc cacacataaa tatatctact gtaactaagt aagcgatttg
     6181 ttcaggtgta aacttcaaac ttgtaccaac aatgattgga actaagatag cacctgcgta
     6241 catagctaaa agatgttgaa cacttaggat taaatttttc attattcttc tcccaccaat
     6301 gtcactttgt ttccttctag tgaagcaacc ttgcagagag aagaaactgt taaacctgct
     6361 tcttctaaac gttgatgccc attttggaaa ctcttttcaa caacaatacc aataccagct
     6421 gtcttagcat tcgcttgctg tgcgatatcg tataatccta atgaagcatc accatttgct
     6481 aaaaagtcat cgatgataag tacagtatct tcttctgata aaaactcttt tgaaacaatg
     6541 accgtacttg ttttattttt agtaaatgaa tgaatagatg tttcataata accatccgtc
     6601 aaagtgctag gttttgcttt tttcgcaaat aaacatggca catcaaaatg cagtgcagcc
     6661 atgattgcag gtgcgatacc ggaagcttca atggttaaga ttttagtaat ccctttatct
     6721 ttaaattgct cgtaaaaagt gcgaccaact tcattcatta actttgcatc aatttgatga
     6781 tttaaaaatc catcgacttt taaaatcttc tcatcaatga caacgccgtc ttcctttact
     6841 ttttgtccta gtaactccac tcttaaaacc tcctcagatt tgtgtgaaac attttgctct
     6901 taaattggtg ctagatacaa aaaaatcccc aaactaaata atagtttcag ggtttatgag
     6961 tgaacgaaca tgcataacga atttgtcatg caatcaatgt aaaagaagtt tcatcaaata
     7021 aactgtgacc atcatataaa atgatataaa tcacccacca tggttacaat ttaatggctg
     7081 aagctactcc tagtattgtg ttgttactca tagtcatgtc gttcaaggca acatggtaga
     7141 aacttctaaa gccatattct ttagattata tgagtttatg taaattattt aacgataata
     7201 gcaaattttc ggcatttttt caataactgc ttaggtaatc ttttaatagt tttataaatg
     7261 tacagagacg tttaatgcga atgaagactt ataacttaat aatgtaaggg ttttacttta
     7321 taataagaag ggtaaaagat taaataaaca aatttttaaa ggggcgacaa ctatggcaga
     7381 tattactgta gtaaatgaca ctggcgaatt atacaacgta atcaatcaaa agaaatctga
     7441 aggttattta gaatcagagc taacaattat tagtaaaagt aaattacacc taaatgactt
     7501 acacgactct gaaatctccc ttatctcaac aagtggaaca tttagtgata gaatgactaa
     7561 actgttaaca ggagaagatg gtgaacatgc agttttatca cgctacaact tagcgcctga
     7621 tgaattagaa aaatataaac aacttatttt agacgataaa atgttagttg tagcggttcg
     7681 tgacaaatca tcacatcaag aagtgcatga aaataattca gcttatgaag agattgatat
     7741 tacacatttt gctgaagcat ctaaaggtcc caaagcttag ttatcacact gaattcaaaa
     7801 tgagattact gtactgtcta atttaagata gtacagtttt ttataccatt taaaagttta
     7861 tgaacagtct tttacagcag gcgattatgc tataatatta tttaaattgg ggaggtgttc
     7921 ttatggaatt taaaaccatt caatctgtca aagatccaca ttacaacgaa gcacttaaac
     7981 tttacgatac taagttaaat ttatctttaa ctgaagataa acgtatcatc aaacgctcat
     8041 tagaaaacaa caaaaccgaa aacgattatg tctttatcgt aggcttagaa aacgatgttg
     8101 ttgtcagtct agcaactgca cattatgaag ccacaaccaa ttcagcattt ttaatttatt
     8161 tattcgcaaa agacctaccc aaccatgatg agttaatgac gcaaacatta catcaaattg
     8221 aacatgaact aaacttatta tcaaaccttg ttcattctag aagtattaac tttttaatga
     8281 tggaagtccc taaagaacct gcagatttaa cacaagataa cggcctcatt attgaacacc
     8341 gtagacagtt tttatacgaa catcaatttg aaagacaaac tgaaattgat tacattcatc
     8401 caactaatga tggtcacact aaaaaagtgg acttattttt aaagaaaaat attaaactga
     8461 caaaggatat ctacggtaca agtgttaaat caaattatat tttgaagtat gtatttgcca
     8521 atggtatcgc acgtgaaatt atttatgctt tattagaaga aatggattta cgtaaaccat
     8581 tttaaacaaa cttgaaatca ctaacaataa cgtttaaact atgtatgaag caagactgac
     8641 gtttcactgc ccgtagacaa acgtcagtat acataaagta tatgcagaaa ggactttaaa
     8701 gtatgttaac taaagaattt gcacaacgcg tagaactaag tgagaagcaa gtacgtaaaa
     8761 ttgttcaaca tttagaagaa cgtggttacc aactaagcaa gactgaatat cgtggtcgtg
     8821 aagcaactga tttcaaagaa gaagatatcg agcttttcaa agacattgcc gacaaagtaa
     8881 aacaaacaaa tagttatgat ctagcgtttg atgaattaga aaaagaaaaa gacttcctgc
     8941 aagtcattgt caaaaacgat gacaaaaact tacctactaa tcaaaatgtc gctcaactag
     9001 tagaagattt acgcctagaa atccagaaaa tgcgcgaaga acgtcaccta cttggtcaaa
     9061 tgatgaatca agtacatcag caacaacaag aattaaaaga acttcaaaat caacttacat
     9121 ctaaaatcga ttcaaatagc gaatccttaa aagccatcca aacatcacaa gaggctatcc
     9181 aagaagcgca agcctctcaa gcaaaagcat tagctgaatc aacaaataaa gttgaaaaga
     9241 atgctgttac tgaagataaa gctgacagta aagattcgaa ggttgctgga gtgaatacat
     9301 caacagacgc taagacggat actaaagcag aaaatgcagg cgatggcact gctactaaag
     9361 tagataaaga ggatcaaatt tctgcaactg aagcaattga aaaagccagc gttgagcagt
     9421 ctaaaaatga caatgcagct gagacttcaa ataaagaagc tacagtagat gcagaagcgc
     9481 aacatgatgc tgaacaacaa gtagcagaag cccatgcaga agcttctaaa caagctactt
     9541 cgaacgattc attggaagcg aaagcagaaa atgacagcac agcttcacag tcagaaatgt
     9601 cagaaccaaa acctcaagaa gagaaaaaag gtttcttcgc acgtttattc aacctgtaaa
     9661 cttgtgattt gaacacaaat ttaatactca attcatatat agctttgatt taaaaactga
     9721 tttttataaa cctatagaac tgtaatttac tttcaaattt aatactcaat tcataaaacc
     9781 tgaaacaagc tgatatactc cattcaatat ctactttgaa gagagcatat caaactaatt
     9841 tgtttcaggt tttttaatta ttcattatga ttaatattga attttgaata aatttttata
     9901 gtatgttact tttaaatggg atggcttctg tgtacttcta aatagattca tcttaataag
     9961 tccattcaat ttaaatgttt ggctgtgtta gtaactgacg aatcgcacaa gtgcacactc
    10021 atattcacat ttactacaaa cgcctttttg ctcaaacaaa aatatttgtt tacggtagct
    10081 tttgaactac cacctgcaca cacgaacact caaaagatgc taattttgat tcgacagaat
    10141 tttttacaac gtgtttgttc gaaacgtctt ttcaaggttg atcatttatt atcttttcat
    10201 tcaataaata tgaggttagc aatttttatc aaaccgccat agccacatct cgattcaagg
    10261 ataaatttgt tttctcatat cttatctctc atgacagtgc acgatatata tcttttccaa
    10321 atattcatca cttttttcct gttaaaatca tttctattat taatgcttta atgacaactt
    10381 atttaattta ttgcatttaa tgccctgctt cattttcaaa tattaacttt aatcgactag
    10441 ctcatcacac tatttaccct ttccacctat cactactgaa ttcaaaacga tttgtttaca
    10501 aatcccctta tgccaacggt cgaatttcat catgtttaca tttaattgca aatgccctct
    10561 tgaaatattt taaaattaat attattctta gcatactagt cggaattata ttcaaaaatt
    10621 tgaggagtga cgttcatgaa tgcttttctt acactgatta atattattgt attggtgatt
    10681 ttcatcgtaa tattgcacat gatggctcgc aaacatatat cttttgcgaa acgtgtgttt
    10741 acagctttag ggattggtat agtattcggt gtgttattgc atcttattta tggcacacat
    10801 tcgaatgtta ttacatcaac aagtgactgg ttcaacattg tcggacaagg ttacgttgca
    10861 ttgttgcaaa tgattgttat gcctttaatt ttcatttcta ttgtagctgc attcactaaa
    10921 attcaaatcg gagaaaagtt tgctaaaatt ggtagtctca tttttatctt tttaattggc
    10981 actgtcacca ttgctgcgat tgttggtgtt gtgtatgcct tggtgtttgg tttagatgca
    11041 tcaacaatta accttggtaa cgctgaacaa gcacgtggaa gcgaaattgc taaacaagcg
    11101 aaagatttaa cagcacatac gttaccacaa caaattttgg aattattgcc taaaaatcca
    11161 ttcttagatt tcactggaca acgtgcaact tccacgattg ctgtcgtgat atttgcttcg
    11221 tttataggat ttgcttattt acgcgtagca cgcaaacaac ctgatcatgg tgaattgctt
    11281 aagcgtgcga ttgacgctat ctattcacta gtaatggcga ttgtgacgtt cgtcttacgt
    11341 ttaacgccat acggtgtatt agcaattatg gccaacacat tatcgacaag tgactttggc
    11401 gccatttgga ctttaggaaa attcttaatc gcatcgtacg ctgcattaat tacgatgtat
    11461 atcattcact taattatttt aagcctgtta ggtattagcc caattcgcta tgttaaaaag
    11521 actttggaag tattaatctt tgcattcaca tcacgctcaa gcgcgggtgc cttaccattg
    11581 aatgtacaaa cacaaacaag acgtctaggc gtgccagaag gtattgccaa tttcgctgcg
    11641 acatttggat tgtcgattgg acaaaatggt tgtgcaggca tttaccctgc catgcttgca
    11701 attatggtcg cacccgtcgc taatgtagaa attgatttac aatttattgt aacgttaatc
    11761 gctgtcgtga ttattagttc attcggcgtt gctggcgtcg gtggcggggc tacatttgca
    11821 tccatattgg tgttgtcaac gttaaacttg ccagtcgctt tagccggcgt gctcatctcc
    11881 gttgaaccat tgattgacat gggtagaaca gcattaaacg tgaacgattc aatgctagct
    11941 ggtacaggta ccgccaagtt aactaaacat tgggataaag atactttcga ttcaaatgat
    12001 aatgctgcat taacttcaca ctaattcatt gcaagaaata cccgccccct ctagctatac
    12061 ttatcatttt aagctagagg ggggcgggtt attttggtat cttgctatcg ctgtattaag
    12121 cctgattttt gcaattgttc taacatatct aatcttgctt tgtttcctag gaacatctca
    12181 atttgctgcg accatgtttc tttgcgattc ccgtttgtac gttgatcata gtactcgctg
    12241 attgtctggt cgtaatctgc catttgtgca tactgtgttt ccttatcagc atgatactta
    12301 ttatgatgga agacatggtc aaatggtaag cgtggcttgg ctgcaccatt ttcgtcatct
    12361 gcgggttccc ctactgccat accaaatacc gggaagacat agtcaggtaa gtctaaaatt
    12421 tctcgaacgc gttcaacatc atttcttaat gatcctaaaa agacaatgcc ataccccata
    12481 tcttcagcag ttaccgcaat attttcggca actaatgctg catcgattgc acctactagc
    12541 aaaccttccg ttgaaccata tgcattttcc atatcagttt cagcatgttg atcaactaaa
    12601 tgatgacgat aataatcaat aacaaagacg aataaatagc cattttctac aacataaggt
    12661 tgtccagaaa cttctcgtaa attttcttta atcttctcat cgtcgatacc aataattgag
    12721 tatgcttgca ggaaacttga cgtcgaagcg ctttgtccag cttctaccaa tttcttaaca
    12781 acgtcttcac ttaaaggttt attcttaaat tttctaacag aatgatgctt tttcacaaga
    12841 ttatatacat gttctgacac gttacccact cctcgatact ttacaattaa tttaaaatct
    12901 tcatattgca attgtacacg tccaaacttt tttatagcaa ttatttgctt ataagttaat
    12961 aattgaactt tttaaaatat tgtctcatac tttaaaaatc cagtttctca ctcattacct
    13021 tcatccatct cgaacaaata ttctcacacc attatcaata aggttgactt agttgagaat
    13081 acaaatcttc tttttttaca ttttttacat taaaatcaag atattaaata tagacacaac
    13141 cttttaaatc atcaactaca aaatccatta tcaaagcact ttatattatc tttctttatt
    13201 ttaaaagtaa tcattcactt tacaaagtta tccagttgac tattctggat caaaatgtaa
    13261 taatgctaac taagagatta gaattattat aattaagcgc aatcaattga tagttctaat
    13321 tcatacttaa attcttagga ggaagatatt tatgtcatta attaacaaag aaatcttacc
    13381 atttacagcg caagctttcg atccaaaaaa agatcaattt aaagaagtta cacaagaaga
    13441 tttaaaaggt tcttggagcg tagtatgctt ctatcctgct gacttctcat tcgtttgtcc
    13501 aactgaatta gaagacttac aaaaccaata tgaagaatta caaaaattag gcgtaaatgt
    13561 attctcagta tcaactgata ctcacttcgt acacaaagca tggcatgacc attcagatgc
    13621 aattagcaaa atcacttaca ctatgattgg tgacccatca caaacaatca ctcgtaattt
    13681 tgatgtatta gatgaagcta ctggtttagc tcaacgtggt acattcatta tcgacccaga
    13741 cggtgttgta caagcatctg aaattaacgc tgacggaatt ggccgtgacg ctagtacatt
    13801 agctcacaaa atcaaagcag ctcaatatgt tcgtaaaaac cctggcgaag tatgcccagc
    13861 taaatgggaa gaaggcgcta aaacattgca acctggttta gatttagtag gtaaaatcta
    13921 aggaggcatt acaaccatgc ttaatgctga tttaaaacaa caacttaaac aactattaga
    13981 actaatggag ggcaacgttg aattcgttgc cagccttggt tcagatgata aatccaaaga
    14041 acttaaagat ttgttgacag aaattactga tatgtcacct agactatctc tttctgaaaa
    14101 atctttaaaa cgtacaccaa gtttctcagt caatcgtcct ggcgaagaaa caggtgtaac
    14161 atttgcaggt attccattag gtcacgagtt taactcactt gttttagcaa ttttacaggt
    14221 tagtggtcgt gcacctaaag aaaaacagtc aatcattgac caaattaaaa aattagaagg
    14281 ttcattccat tttgaaacat tcattagttt aacgtgtcaa aaatgtcctg atgtcgttca
    14341 agcacttaac ttaatgagtg tgatcaaccc taacatcacg cattctatga ttgatggtgc
    14401 agtgttccgt gaagaatctg aaaacatcat ggcagtccct gctgtctttt taaatggcga
    14461 agaatttggc aatggtcgta tgacaatcca agatattctt tcgaaactag gcagtacggc
    14521 agatgcatct gagtttgaaa ataaagaacc ttatgatgtc ttaatcgttg gtggtggtcc
    14581 tgctagtggt agtgcagcga tttacacagc acgtaaaggt ttacgtactg gtatagttgc
    14641 tgatcgtatc ggtggccaag ttaatgatac tgctggtatt gagaacttca ttactgttaa
    14701 agaaacaact ggttctgaat tttcttctaa cttagcagcg cacattgatc aatatgacat
    14761 tgatgcaatg acaggtatac gtgctacaga tatcgaaaag actgacgaag caattaaagt
    14821 tacgttagaa aacggtgctg tcttagaaag taaaacagtc attattgcta ctggtgcagg
    14881 ttggcgtaag ctaaacattc caggtgaaga gcaattgatt aataaaggtg ttgcattctg
    14941 ccctcactgt gacggacctc tatttgaaaa taaagacgta gcagttatcg gtggcggtaa
    15001 ctctggggtt gaagcagcaa ttgaccttgc tggtatcgtt aatcatgtta cattattcga
    15061 attcgctagc gaattaaaag cagacaacgt gttacaagat cgtttacgtt ctttatcaaa
    15121 tgttgatatc aaaacaaatg ccaaaactac tgaagttgtc ggagaagacc atgttacagg
    15181 tatacgttac gaagacatga acaccggcga agaacatcta cttaacttag atggtatctt
    15241 tgttcaaatt ggtttacttc caaacacatc atggttaaac gatgctgttg aattaaacga
    15301 acgtggtgaa attgtgattg atcgtaacaa taatacgaat gttcctggaa tatttgctgc
    15361 tggcgatgtc acagatcaga agaacaaaca aattatcatt tcaatgggcg ctggtgcaaa
    15421 tgcagcatta aatgcctttg actatattat cagaaactaa agtttcattc atttcactga
    15481 cagtatctta catttgttaa gatgctgtta tttttttatc ccgacataaa atcatgttat
    15541 gataaactta atatatacaa cgagatgtgc gataatgctt aaagtatgta cctctcgcaa
    15601 acaaatcaat atgaaaaatg atattgagaa agaaaagtat gtgaacgaaa ttattatgct
    15661 tttacgtacg atacctacgt ctaaattagt acaaccaatt atgcagttat ttatagataa
    15721 agctatcctt aacaacgtcc aaaatgatgc attattgata gaaaaagcaa ctcatgcacg
    15781 taatcattta cgtctatggt cattgcaagg tcatcgtgac attccaaact aattcaatgt
    15841 tactctatta aacatgaata catatttttt caattaaaga taaactaagg agttatatta
    15901 cattatgaaa aagagattac tactaagtac atttttagca tcgacattaa ttcttacagg
    15961 ttgcgcgtcc gatcaatctg ataacgaaga tcatcataca agtactggca tccatgcacc
    16021 taaaagcgct aaaaaattag aaacaaaaga tatctttaac tcagataaga aaaatagtga
    16081 tattagtgat gcagagatga agcaagctat agaaaagtat ttgtctgtaa atagcgatat
    16141 acttgataac aaatatatta tgcaacataa actagataaa caaattgaca gtcaaacaaa
    16201 agtgactgaa aaacaagcag aaacacttag ccacttatct aacttagctg ttaaaaacga
    16261 tttgcatttc aagaaatttg tcactgaaaa caatattcct aaagaatata agaaacccgt
    16321 tgaattaatg atgaattact tcaaagcttt aaatagtaca atcgccaatg ttgatgaaga
    16381 tattgaaaag cttagctatc aaccgcaaaa taaaatcaat gttgtcgacg tgccaacaaa
    16441 atatgccggt gatgttaata aaaagcaaca agataaaatt aaagatttct taaaatctaa
    16501 aggtatcaag agtgatgtta tcgataaata actgaaagcc atcattgcga aatgtaacac
    16561 atcattacat gagttttaat tcatgacaat tctttatctt taaaagtgac taatcaattt
    16621 agctaaagtc atcatttggg catcaagcag catattactg tttgatgcct ttttaattgc
    16681 cttaaattct ctatctatgc caaaattccc attagttgac gcatttgact atatacctaa
    16741 catattccat acttatattg actctataaa caaaggatga tcatgatgaa agttcgaatt
    16801 acttttatta ttttagcaat tttatcgacg attgtttgct tgttcttagc agcaatgcat
    16861 ccaacaggac caaacacagt aacttttgaa cagccatatt tattcaccct caacatcatt
    16921 ataatggtat tagtcgcatt accttcactt gttttagcta tatatgatta tatgagtttt
    16981 agaatttctt ctgctatttt acaatttcta ggggctatct cttggttctt tttatcattg
    17041 atattatcgc tcacacaatt tacacctttt acattagcgt catttataac ttcaattatt
    17101 ttgttcacaa gcacaattat cacattagcc attggtggta agtctgttga aaagaatgat
    17161 tccccttaaa ttccaaatga aaaaaaggtt ctgaaggccg ctataaaaca cagtttttca
    17221 gaacctctat acttctattc aatgatatat ggtttgcaat tttctacctt taaatccaca
    17281 gcttctgccc ttgaaacttt gttaaaataa accatcaaac aacgaatgac aacttgatgt
    17341 gcaacaatga caatatcatc tttttgtgta tcttcattga caacatgatt cataaaatgt
    17401 tctacgcgtt gatatacatc ttcataactt tctccttcag gcgctttttg tgaaaaacta
    17461 tgacgaaagt ctttaaagtt tggatcattg aaatatttat catatttcgg attcgcactg
    17521 atttcatctt tatattcacc ctcaaatacg ccaagtgaac gttctcttaa tagaggggta
    17581 gtcgttgatg caatgtcata tggaaaaata tgttcaaacg tttgctgtgt tcttaataag
    17641 tctgaaacat atacatgttt aatctgtttc tctttaaaat aatcacataa atcgtctgca
    17701 ctttttgtgc cagtatccgt taacggcaca tctaattgtc cacaaaaata agatcgaaaa
    17761 tgtttattat cataattcga ttttgattcg ccatgtctaa ctaaataaat cgtcataata
    17821 ttactcctta ccttatgtat ttcatatcta ccataacact ttgactacta attcgatatc
    17881 aatcttaata ttctattcta aaaaaagaat taattcatat tttaaattaa tgactctaaa
    17941 aatagtattg tctttaatca agtgatgtgt tactatatgg gtgtagttct taaatgacta
    18001 cacagtcttt tatattcatg aacataatac tcctggatgg cgcaacccaa cgtcatcctt
    18061 tttttatttg tcaaatactc gcttttttat ttccaaaatt tcctcatatt aatttattac
    18121 tttttaatcc tatcttatct acattttcaa atattataat acatattgac cattttcacg
    18181 caaataaaaa agatgacaac tgaatgccat ctttatttca atttccaatt gaattttttt
    18241 attatttacg catagctctt aaaattaacg ttacgattgc aattaagata attgaaccaa
    18301 ttaatgctgg caagatgtaa atacttccta attcaggacc ccattgtccg aatagtttgc
    18361 cacctaccca tgatccaata atacctgcga taatattgcc taaaatacca cctgggatat
    18421 ctttacccat aatagcacca gcagcccatc caattaagcc accgacaatt aacattccaa
    18481 taaatccaaa cataattttc agtctccttt ttctatttat tttgcgttat tctaagtagt
    18541 accccttatt tacaattcta aaacaaattc aaattatttt tatccaaata tttttaaaag
    18601 tagtaattga atatcaattt tattcaatgt agctatcgtt atttaaagtc tctgtaccga
    18661 taatatcata tacatttaca ttattttttc tgccgaattc atagcttgat tattttatgt
    18721 tataggacta gaatatacac atattattag agcatctttg aattttaaat caagaagcga
    18781 ggttaatgaa caatgaatat gcatatttta tataacttac gaactaaaca taatttagaa
    18841 attgacgaat tagcacagca attaaatgag aaatatggta ctaaatatga agcacatcaa
    18901 atttgggaat gggagaatca tcaccatgaa cctaaattta aagatgccat gcatttagct
    18961 gacttctttg atgcaccata tgaaatgttt ttagaaagta aggttaaaga atatcagaaa
    19021 catttagaag aagtcgatat tcgcatggat aaatagatgc aaataaaccc tcacaacacg
    19081 tttggcatat atcctttcaa atctatactg gatatattac attacgttgt gagggtattt
    19141 tattaattaa tatgaattaa gacattttac aagcgttaat gcaacgaatc tttttagtga
    19201 tcttgctcac tctttaatac tttaccgttc ttagcatcaa cagtaacttc ttgtttttta
    19261 ttaccttttt tcaaatcgat attgtaaaca agtttgccat catctttttc aagtgaccat
    19321 tctttaatat caccatcaaa ttctttttgt ccttctttaa tggctttttt gtaatctata
    19381 gcatcgctat atttaaagtt atcattttca ttcattgtat cttctttttc agtctttttg
    19441 ttaatcactt ttttattttt atcagcaaca agtacttctg actcttcacc agatttttgt
    19501 tgcgtcactt tataagccca ttcaccatta gaattttcaa atgaaattcc tttcaacttt
    19561 tggcctttgt aagtttcttc agcttttttc acagcatctt ctgggcttgt tttaacatct
    19621 tttaaagcaa taacatcttt tgttgtatta gtgtcttgat tagtatttga ctctgttgat
    19681 tttgtttcat cttttggagt atcattgcca catgcagtaa gcaccaccgc tgacattgat
    19741 aacactgcta atgattttaa tttcataata tcactctctc ttcctatttt tgaaactcat
    19801 aacaaaagct tatatgctat atagattgta ttaccccttg tttttaattt tattcataat
    19861 tattacaaat atttttaaat taatcgtcat gcgttacttt cgttcgtact ccttttataa
    19921 atgaaccatg taatataagc atgctattat cgactcagtt tgtctaatgc tttctttggt
    19981 acttcttcct tttcaacttc ttcaaaagtt tctacatgat gacctttatg tgtaattttc
    20041 aagtatctat gcggtttaac atcaaatgta gcagtataca ttaattgcgt ctctttccct
    20101 tctttattaa aaacactttg actataactt cggagttgat catccattct agtagacacc
    20161 tctgtcgttt taacataaga atcatccttc tttactaatg ggttaaattg atctgttata
    20221 ccatgggagt ctattgtttt taaaatgaat aaagcagcat aagcgccaat gatagtaagt
    20281 acaagatatg ttattgtttt taaaatcgtt ttcaaaagat cacctctgac aaaatataaa
    20341 ataggatgta tctgaaaaca acttacataa ttgtaattaa agtgtaagta ttaggagaaa
    20401 atgatggtat ttgaatctga aagatacgaa tcgtttttaa actaagttat tttatagaga
    20461 ctttattatg aaattgaaag aagttaattt taaggttgtc gaatgtaaaa acatattgat
    20521 taaccataaa agagaaatta tttttgtaga tttcggcata aacgtcatta aagaattttc
    20581 tatatgatgg tgctaatcat aacaaagaaa gctaggcacc tttagatacc taactttcaa
    20641 agtattagcg ataaaacact atatatacta atttagctta atttcaaact tgttcaatgt
    20701 cattaacact tttcacaagt ttgaaagtta cattttttat atcggctgta ttaattttta
    20761 cattttccct atctttttgt agctctttat gcgactctaa tgtatattta ccaccatctt
    20821 ttgtatttat gacgatgtta cctttaggca aatgttctcc catatacaag ccataggaaa
    20881 tatgagcaaa tcttatgata tgatccaatt cttttaaagt gacaacttct ttattaagag
    20941 taatattcct cttaggtgta tacactctgc cagcaactgt gtatgttcct tctaaatgta
    21001 tacgaacttg gttttcattt aaaggtctct ctgaataaac ccaattccta gatttattag
    21061 attttccatt tattgtatcc ttgctgtatc tatcgtaaag ttttttaacc aaagcttttt
    21121 ctgaattatt tggtacttct tcaacttttt gtaattgttt atcttgtaca cttgaggaat
    21181 tttgtgttga cgcgtctgct tttggatata ttccaaatgt tcccccatag atgataccta
    21241 gcgatagaat agattttaat acgattgaat tttttgagtc atattttttg aacatattta
    21301 attacctcct tgatgtaaag ctttatttgc tacaattata aaaataatag acgtgttcat
    21361 gaattaaatt catcttaact cttgattaac tttaatttgc taccactctg aatttaataa
    21421 ctataaatcg tctacacata attggacaaa atctaagaga ataaaatttg ttaatttaaa
    21481 atagcaagca attcaaagtt atatgt
//
LOCUS       DANRDI010000032        20867 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.032, whole genome shotgun sequence.
ACCESSION   DANRDI010000032 DANRDI010000000
VERSION     DANRDI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20867
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.032"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..64
                     /locus_tag="PZ377_000931"
     CDS             <1..64
                     /locus_tag="PZ377_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDM6357816.1"
                     /translation="PSDFAKLSDQSDLMRAEVSE"
     gene            65..211
                     /locus_tag="PZ377_000932"
     CDS             65..211
                     /locus_tag="PZ377_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357817.1"
                     /translation="MMWLIIAIILLVILLFGVMLQAEQLKGDVKVKEREIEILRSRLR
                     HFED"
     gene            235..657
                     /locus_tag="PZ377_000933"
     CDS             235..657
                     /locus_tag="PZ377_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RinA family phage transcriptional activator"
                     /protein_id="HDM6357818.1"
                     /translation="MTKKKYGLKLSTVRKLEDELCDYPNYHKQLEDLRSEIMTPWIPT
                     DTNIGGEFVPSNTSKTEMAVTNYLCSIRRGKILEFKSAIERIINTSSRKEREFIQEYY
                     FNKKELVKVCVDIHISDRTAHRIKRKIISRLAEELGED"
     gene            844..1284
                     /locus_tag="PZ377_000934"
     CDS             844..1284
                     /locus_tag="PZ377_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDM6357819.1"
                     /translation="MNEKQKRFADEYIMNGCNGKKAAITAGYSKKTAESLASRLLRNV
                     NVSEYIKERLEQIQEERLMSITEALALSASIARGEPQEAYSKKYDHLNDEVEKEVTYT
                     ITPTFEERQRSIDHILKVHGAYIDKKEITQKNIEINIGEYDDES"
     gene            1271..2548
                     /locus_tag="PZ377_000935"
     CDS             1271..2548
                     /locus_tag="PZ377_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDM6357820.1"
                     /translation="MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHG
                     VIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNK
                     VELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQ
                     IFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPA
                     YYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDV
                     KKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILP
                     TKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHC
                     IDSLRYSVERFYRPVRKRTNLSSKVDTIKSLGL"
     gene            2559..4094
                     /locus_tag="PZ377_000936"
     CDS             2559..4094
                     /locus_tag="PZ377_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDM6357821.1"
                     /translation="MLKVNEFETDTDLRGNINYLFNDEANVVYTYDGTESDLLQNVNE
                     VSKYIEHHMDYQRPRLKVLSDYYEGKTKNLVELTRRKEEYMADNRVAHDYASYISDFI
                     NGYFLGNPIQYQDDDKDVLEAIEAFNDLNDVESHNRSLGLDLSIYGKAYELMIRNQDD
                     ETRLYKSDAMSTFVIYDNTIERNSIAGVRYLRTKPIDKTDEDEVFTVDLFTSHGVYRY
                     LTSRTNGLKLTPRENGFESHSFERMPITEFSNNERRKGDYEKVITLIDLYDNAESDTA
                     NYMSDLNDAMLLIKGNLNLDPVEVRKQKEANVLFLEPTVYADSEGRETEGSVDGGYIY
                     KQYDVQGTEAYKDRLNSDIHMFTNTPNMKDDNFSGTQSGEAMKYKLFGLEQRTKTKEG
                     LFTKGLRRRAKLLETILKNTWSIDANKDFNTVRYVYNRNLPKSLIEELKAYIDSGGKI
                     SQTTLMSLFSFFQDPELEVKKIEEDEKESIKKAQKGIYKDPRDINDDEQDDDTKDTVD
                     KKE"
     gene            4101..5096
                     /locus_tag="PZ377_000937"
     CDS             4101..5096
                     /locus_tag="PZ377_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDM6357822.1"
                     /translation="MPNKNTQEYWEERGRKAIENELKRDKTKAEEIERILNMMIKRIE
                     KEINAFIVKYGDFAGVTLQEAQKIIDEFDVKAFQEEAKRLVENKEFSDRANEELKKYN
                     TKMYVSREQMLKIQIEFLIAYATAQTELSMREYFESTAYRVFSDQAGILGEGVQVAKE
                     VIDTIVDTQFHGVVWSERLWTNTEAMKQEVEEIIANVVIRGRHPNEYVKDMRKHLNKF
                     EGTARQKTAAIKSLLYTESARVHAQSSIDSMKEISPEGYYMYIAKIDNRTTKVCKGLN
                     GEIFKVKDAKIGVNFYPMHINCRSDCALLPKSMWPKKPSKKRKTKYFGGKVKSGD"
     gene            5169..5339
                     /locus_tag="PZ377_000938"
     CDS             5169..5339
                     /locus_tag="PZ377_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357823.1"
                     /translation="MSNTDKYLRDIARELKGIRKELQKRNETVIIDANLDSLRSAVLA
                     DKEKSKYNEPLF"
     gene            5448..6068
                     /locus_tag="PZ377_000939"
     CDS             5448..6068
                     /locus_tag="PZ377_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDM6357824.1"
                     /translation="MEENKLKFNLQFFADQSDDPDEPGGDGKKGNPDKKENDEGTEIT
                     FTPEQQKKVDEILERRVAHEKKKADEYAKEKAAEAAKEAAKLAKMNKDQKDEYEREQM
                     EKELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKLNVEAFSNAV
                     KKAVNEAVKVNARQSPLTGGDSFNHSTKNKPQNLAEIARQKRIIKN"
     gene            6082..7056
                     /locus_tag="PZ377_000940"
     CDS             6082..7056
                     /locus_tag="PZ377_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDM6357825.1"
                     /translation="MEQTQKLKLNLQHFASNNVKPQVFNPDNVMMHEKKDGTLLNDFT
                     TPILQEVMENSKIMQLGKYEPMEGTEKKFTFWADKPGAYWVGEGQKIETSKATWVNAT
                     MRAFKLGVILPVTKEFLNYTYSQFFEEMKPMIAEAFYKKFDEAGILNQGNNPFGKSIA
                     QSIEKTNKVIKGDFTQDNIIDLEALLEDDELEANAFISKTQNRSLLRKIVDPETKERI
                     YDRNSDSLDGLPVVNLKSSNLKRGELITGDFDKLIYGIPQLIEYKIDETAQLSTVKNE
                     DGTPVNLFEQDMVALRATMHVALHIADDKAFAKLVPADAKPSSNPGEV"
     gene            7078..7365
                     /locus_tag="PZ377_000941"
     CDS             7078..7365
                     /locus_tag="PZ377_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357826.1"
                     /translation="MPEIIGIVKVDFTDLEDNRHVYMKGHVYPRKGYDPTDERIKALA
                     SVENKRNEQMIYIVNDKLTKKELVEIASVAGLQVDEKQTKAEIINAFESLE"
     gene            7374..7706
                     /locus_tag="PZ377_000942"
     CDS             7374..7706
                     /locus_tag="PZ377_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDM6357827.1"
                     /translation="MTTLADVKKRIGLKDEKQDEQLEEIIKSCESQLLSMLPIEVEQI
                     PERFSYMIKEVAVKRYNRIGAEGMTSEAVDGRSNAYELNDFKEYEAIIDNYFNARTRT
                     KKGKAVFF"
     gene            7703..8005
                     /locus_tag="PZ377_000943"
     CDS             7703..8005
                     /locus_tag="PZ377_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357828.1"
                     /translation="MRYEDRAVFQSEQAATYNPKTSKKENALITYDAIPCNINPISRA
                     RKQLEFGDVKNDVSVLRIKESISYPVSHVLVNGIRYKIVDTRIYRHETSYYIEEVN"
     gene            8005..8352
                     /locus_tag="PZ377_000944"
     CDS             8005..8352
                     /locus_tag="PZ377_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDM6357829.1"
                     /translation="MNIDGLDALLNQFHDMKTNIDDDVDDILQENAKEYVVRAKLKAR
                     EVMNKGYWTGNLSRNIRYKKTGDLQYTITSHAAYSGFLEFGTRYMEAEPFMWPVYEVI
                     RKSTVEELKALFE"
     gene            8364..8747
                     /locus_tag="PZ377_000945"
     CDS             8364..8747
                     /locus_tag="PZ377_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357830.1"
                     /translation="MTPNLQLYNKAYETLQGYGFPVISRKEMQQEIPYPFFVIKMPES
                     NRSKYTFDSYSGDTNLVIDIWSVSDDLGHHDGLVKRCIDDLTPSVKTNDYDFEEDDTN
                     IAQLVDDTTNQELLHTSITISYKTF"
     gene            8766..9347
                     /locus_tag="PZ377_000946"
     CDS             8766..9347
                     /locus_tag="PZ377_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HDM6357831.1"
                     /translation="MANMKNSNDRIILFRKAGEKVDATKMLFLTEYGLSHEADTDTED
                     TMDGSYNTGGSVESTMSGTAKMFYGDDFADEIEDAVVDRVLYEAWEVESRIPGKNGDA
                     TKFKAKYFQGFHNKFELKAEANGIDEYEYEYGVNGRFQRGFATLPEAVTKKLKATGYR
                     FHDTTKADALTGEDLTAIPQPKVDSSTVTPGEV"
     gene            9409..9774
                     /locus_tag="PZ377_000947"
     CDS             9409..9774
                     /locus_tag="PZ377_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDM6357832.1"
                     /translation="MNVEINGKSLELSFGFKFLREIDNRLGLKVEQASIGQGVSMLPV
                     GLESGNPVVIGEVLIAATSHLKKQAITINNIDEALDEIAENIGLEEFGSDILTELGKR
                     PMTRNLVEVVETEEKPAEA"
     gene            9804..10148
                     /locus_tag="PZ377_000948"
     CDS             9804..10148
                     /locus_tag="PZ377_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357833.1"
                     /translation="MSTLGITDLNVIEQMTLTEYNYRMYAKEYEMLTQEFERYKLAFA
                     IRDAAATKNVGTENKPKEEYVFNNANDVLPYEENIQRLNEGKDIRFSSERDEYEPQNN
                     EFFKVIAEFNKQ"
     gene            10165..13632
                     /locus_tag="PZ377_000949"
     CDS             10165..13632
                     /locus_tag="PZ377_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357834.1"
                     /translation="MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELN
                     ADDKKLQKTIKVAKKSLDAFSNKKVKAKLDASIQDLQQKVLESNFELDKLNSKEVTPE
                     IKLQKQKLTKDIAEAEAKLSELEKKRVNIDINADNSKFNRVLKVSKASLEALNRSKAK
                     AIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKT
                     TKVDVDTNGLKKAYAYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILI
                     PVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLNDGTLQ
                     ASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRG
                     MEEASQSVLKWAENSSVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLF
                     QWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNA
                     FSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLV
                     AFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIPIILAV
                     SNFMGGGLIGRIIALVSKFGLLRAGLTILKGAFMLLKGPLKIISVIFQLLFGKIGLIR
                     NAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVT
                     VWNVLKTVASVVWNAISKAITTAVTNVYNFIMIVWNQIVAYLQGLWNGIIAIATTVWN
                     LLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIM
                     TIAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTAISTALQAAWSFISNIWNTIWSFL
                     SGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVIQG
                     FVNVVNKVSQGMTNAVNKIKSFIGDFVSAGADMIRGLIRGIGQMAGQLVDAAKNVAKK
                     ALDAAKSALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPT
                     LNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKIEFDVNNDALTSIVNGRN
                     AKRNSEYYL"
     gene            13645..14592
                     /locus_tag="PZ377_000950"
     CDS             13645..14592
                     /locus_tag="PZ377_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDM6357835.1"
                     /translation="MDIELTKKDGTVIKLSEYGFIVNDIVIDSMQINTKYQDKENMNG
                     RILMGSNYISRDIVVPCFCKVKNRSDIAYMRDMLYSLTTDIEPMYLREIRRKEELNYR
                     FTQPTSDDYVKLDKNNFPDYEYSRHDQQIYVNGKQYKVIFSGVINPKQKGNKVAFELK
                     FETTELPYGESIGTSLELEENKKVGLWSFDFNIDWHAGGDKRQYTFENVSKDTVYYHG
                     SAPNDQFNMYKKITIILGEDTESFVWNLTHAEIMKIEGIKLKAGDKIVYDSFRVYKNG
                     VEISTETNIAQPKFKYGANKFEFNQTVQKVQFDLKFYYK"
     gene            14601..16502
                     /locus_tag="PZ377_000951"
     CDS             14601..16502
                     /locus_tag="PZ377_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDM6357836.1"
                     /translation="MTITVKPPKGNGAPIPVETTLVKKVNADGVLTFDILENKYTYEV
                     INAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVN
                     VTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYK
                     ITYDKKKDRYKFVLTPFANQKASYFISDEVNANAIKLEEDASDFATFIRGYGNYSGEE
                     TFEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDF
                     LVLRESYPEADPQPGDIVQIKSTKLGLNDLVRIVQVKTIRGINNVIVKQDVTLGEFNR
                     EQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQ
                     QKVSSKTVTTSDGTIVHDFIDKSNIKDVKTIGTIGDSVARGSHAKTNFTEMLGKKLKA
                     KTTNLARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIG
                     TDKTDTKTFYGAFCSAIEVIRKNNPASKILVMTATRQCPMSGTTIRRKDTDKNKLGLT
                     LEDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYEL
                     IKNYYQFYG"
     gene            16517..18427
                     /locus_tag="PZ377_000952"
     CDS             16517..18427
                     /locus_tag="PZ377_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357837.1"
                     /translation="MDNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRN
                     KEVFAHNGEQIKYEHLNVTSSVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGY
                     DHKTLQDRLYHDYSTLDTFTKKVEKAVDEHYKEYQATEYRFEPKEQEPEFITDLSPYT
                     NAVMQSFWIDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYI
                     DGELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPIENL
                     MIFRREYKASERQAKNSLNFIEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITY
                     DAGILYWYTGDSNTANPNYLQGFDVKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMY
                     YDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLP
                     IQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLT
                     RNSTGRNMLKFERVIDIFNKKNNGSWNFNPQSAGYWEHIPKSITKLSDLKIVGLDFYI
                     TTEESKRFSDFPKDYKGIAGWVLEVKSNTPGNTTQVLRRNNFASAHQFFVRNFGTGGN
                     SGWSIIEGKEVE"
     gene            18427..20250
                     /locus_tag="PZ377_000953"
     CDS             18427..20250
                     /locus_tag="PZ377_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDM6357838.1"
                     /translation="MVVDNFSKDDNLIELKTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTVVDAINGRLQYVIPNEFLK
                     HSGKVHAQAFFTQHGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGK
                     DFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFNK
                     IVEKEQTIFARVNEVEQQINGADLVKGNSTVNWQKSKITDDYGKAIESSEQSIDSVLS
                     TVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEEISNNALNQAKQYVD
                     GKFQSTSWQQHKLTEHNGQSIQKNLYNAKGNLEALGAGNYYVTSVPDLPGIVESYEGY
                     LSVFVKDDANKLFNFTPSNSKKVYTRSITNGRLDSQWAAPNEHKTAVLFDGAANGVGT
                     RINLTEAYTNYAILFISGTYPGGVIEAFSLTSIPNAIQLSKTNVVDSDGNGGGSYECL
                     ITKESGTTLKIDNDVYLDLGSKTGSGANANRVTINKIVGWK"
     gene            20250..20627
                     /locus_tag="PZ377_000954"
     CDS             20250..20627
                     /locus_tag="PZ377_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDM6357839.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASKQQSESDLSDEELRGMVASMQMQMTQVNMLTMQLTQ
                     QNAMLTQQLTELKTNKTNTEGDV"
     gene            20631..20804
                     /locus_tag="PZ377_000955"
     CDS             20631..20804
                     /locus_tag="PZ377_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDM6357840.1"
                     /translation="MKMIYPTFKDIKTFYVWGCYKNEQIKWYVDMGVIDKEEYALITG
                     EKYPETKDEKSQV"
BASE COUNT         7780 a         3030 c         4214 g         5843 t
ORIGIN      
        1 gccgagtgac ttcgcaaagt tgagcgatca gtctgatttg atgagggcgg aggtgtcgga
       61 gtagatgatg tggttaatca tagcaattat attactagtc atcttattgt ttggtgtgat
      121 gttacaagcg gaacagttaa aaggtgatgt aaaagttaaa gagcgagaga tagagatatt
      181 aagaagtaga ttgagacact ttgaagatta aacatatttg tacggagggt attcatgact
      241 aaaaagaaat acggattaaa attatcaaca gttcgaaagt tagaagatga gttgtgtgat
      301 tatcctaatt atcataaaca acttgaagat ttaagaagtg aaataatgac accgtggatt
      361 ccaacagata caaatatagg cggggagttt gtaccgtcta atacatcgaa aacagaaatg
      421 gcagtaacta attatctttg tagtatacga agaggtaaaa tccttgagtt taagagcgct
      481 attgaacgta taatcaacac atcaagcagg aaagaacgcg aattcattca agagtattat
      541 tttaataaaa aggaattagt gaaagtttgt gttgacatac acatttctga tagaactgct
      601 catagaatca aaagaaaaat catatctaga ttggcggaag agttagggga agactgaaat
      661 tggcagtaaa gtggcagttt ttgatacctt aaatgagata ttatgatagt gtaggatatt
      721 gactatcgta ctgcgtttcc cttatcgcaa ttaggaataa aggatctatg tgggttggct
      781 gattatagcc aatccttttt taattttaaa aagcgtatag cgcgagagtt ggtggtaaat
      841 gaaatgaacg aaaaacaaaa gagattcgca gatgaatata taatgaatgg atgtaatggt
      901 aaaaaagcag caattacagc aggttatagt aagaaaacag cagagtcttt agcaagtcga
      961 ttgttaagaa atgttaatgt ttcggaatat attaaagaac gattagaaca gatacaagaa
     1021 gagcgtttaa tgagtattac agaagcttta gcgttatctg cttctattgc tagaggagaa
     1081 cctcaagagg cttacagtaa gaaatatgac catttaaacg atgaagtgga aaaagaggtt
     1141 acttacacaa tcacaccaac ttttgaagag cgtcagagat ctattgacca catactaaaa
     1201 gtacatggtg cgtatatcga taaaaaagaa attactcaga agaatattga gattaatatt
     1261 ggtgagtacg atgacgaaag ttaaattaaa ctttaacaaa ccgtctaatg ttttcaatag
     1321 aaacatattc gaaatactaa ccaattacga taacttcact gaagtacatt acggtggagg
     1381 ttcgagcggt aagtctcacg gcgttataca aaaagttgta ctcaaagcat tgcaagattg
     1441 gaaatatcct aggcgtatac tgtggcttag aaaagtacaa tcaacaatta aagatagttt
     1501 gttcgaagat gttaaagatt gtttgataaa ctttggtatt tgggacatgt gcctttggaa
     1561 taagactgat aacaaagttg aattgccaaa cggcgcagtt tttttgttta aaggattaga
     1621 taacccagag aaaataaagt cgataaaagg catatcagac atagtcatgg aagaagcgtc
     1681 tgaattcaca ctaaatgatt acacgcaatt aacgttgcgt ttgagggagc gtaaacacgt
     1741 gaataagcaa atatttttga tgtttaaccc agtatctaaa ctgaattggg tttataagta
     1801 tttctttgaa catggtgaac caatggaaaa tgtcatgatt agacaatcta gttatcgaga
     1861 taataagttt cttgatgaaa tgacacgaca aaacttagag ttgttagcaa atcgtaatcc
     1921 agcatattac aaaatttatg cgttaggtga atttgctaca ctagacaaat tggttttccc
     1981 taagtatgaa aaacgtttaa taaataaaga tgagttaaga catttacctt cttattttgg
     2041 attggacttt ggctacgtta atgatcctag tgcttttata cattctaaaa tagatgtaaa
     2101 gaaaaagaag ttatacatca ttgaagagta tgttaaacaa ggtatgctga atgatgaaat
     2161 agctaatgtc ataaagcaac ttggttatgc taaagaagaa attacagcag atagtgcaga
     2221 acaaaaaagt atagctgaat taaggaatct agggcttaaa aggattttac caaccaaaaa
     2281 agggaagggc tcggttgtac aagggttaca attcttaatg caatttgaaa tcattgttga
     2341 tgaacgttgt ttcaagacta ttgaagagtt tgacaactac acatggcaaa aggacaaaga
     2401 tacaggtgaa tataccaatg aaccagtaga tacatacaat cattgtatcg attcgttgcg
     2461 ttattcagtg gaacgattct acagaccggt tagaaaacgc acaaatctca gttcgaaagt
     2521 tgacacaata aaatctctag gattatagga gggaacaaat gttaaaagta aacgaatttg
     2581 aaacagatac agatctacgg ggaaacataa attacttatt taatgatgaa gccaatgttg
     2641 tttacacata tgacgggacg gaatccgatt tattacaaaa cgttaatgaa gtaagtaaat
     2701 acattgaaca tcacatggat taccaacgac ctagattgaa agtgttaagt gattattacg
     2761 aaggtaaaac taagaactta gttgagttaa cacgacgcaa agaagagtac atggcagata
     2821 accgtgtagc gcatgattac gcatcttata ttagcgattt tatcaacggc tatttcttgg
     2881 gtaatccaat tcaatatcaa gatgatgaca aagatgtatt agaagctatt gaggcgttca
     2941 atgatttaaa tgatgttgag tcacacaata gatctttagg attagatttg tcaatttatg
     3001 gcaaagctta tgagttaatg attagaaacc aagatgatga aacgcgttta tacaagagtg
     3061 atgcaatgag tacttttgtc atatacgaca atacaattga acgtaatagt atcgcaggag
     3121 ttagatattt aagaactaaa ccaatagaca agactgacga agatgaagtg tttacagttg
     3181 atttattcac ttcacacggt gtttatagat atcttaccag tagaacaaat ggattgaagc
     3241 tcacaccacg tgaaaacggt tttgaatcac actctttcga acgtatgcct attacagaat
     3301 ttagcaacaa cgaaagaaga aaaggggatt atgagaaagt aatcacttta attgatttgt
     3361 atgataatgc tgaatcagat actgctaact atatgagtga tttaaatgac gctatgttac
     3421 ttattaaagg taatttaaat ttagatcctg tagaagttag aaaacaaaag gaagctaacg
     3481 tgttgttttt agaaccgact gtttatgctg atagcgaagg tagagaaaca gaaggctctg
     3541 ttgatggtgg ttatatttat aagcaatacg atgtacaagg taccgaagct tataaagacc
     3601 gtttaaacag tgatatacac atgtttacca acacgcctaa catgaaagat gataacttta
     3661 gcggcactca atcgggcgag gcaatgaaat acaaattatt tggattggaa caacgtacta
     3721 aaactaaaga aggattgttt actaaagggt taagacgtcg tgctaagttg ttagagacaa
     3781 tacttaaaaa tacatggtcg attgacgcta acaaagattt caatactgtt agatacgtat
     3841 acaacagaaa cttacctaaa tcattgattg aagaattaaa agcttatatt gattctggtg
     3901 ggaagattag ccaaacaact ttaatgtctc tattctcgtt cttccaagac cctgaattag
     3961 aagttaagaa aatcgaagaa gatgagaaag aatctattaa aaaagctcaa aaaggtattt
     4021 ataaagaccc tagagacatc aatgatgacg aacaagatga tgatacaaaa gatactgttg
     4081 ataaaaagga atgattgtaa ttgcctaaca aaaacactca agaatattgg gaagaacgcg
     4141 gacgcaaagc aatcgagaat gagttgaagc gtgataaaac taaagctgaa gaaatagaac
     4201 gtatattgaa tatgatgatt aagcgcattg aaaaagagat caatgcgttt attgtcaagt
     4261 acggagattt tgcaggcgtt acattacaag aagcacaaaa gattattgat gagttcgatg
     4321 taaaagcgtt tcaagaagaa gcaaaaagat tggtcgaaaa caaggagttt agcgatagag
     4381 caaatgaaga attaaagaag tataacacga aaatgtatgt atctagagaa cagatgttaa
     4441 agattcaaat agaattctta attgcttatg caacagctca aacagaatta tcgatgaggg
     4501 aatatttcga atcaacagct tatcgtgtgt tcagtgatca agcgggtatt ttaggtgaag
     4561 gtgtacaagt agctaaagaa gttatagata caatcgttga tacacaattt catggtgtcg
     4621 tttggtcaga gcgattatgg actaataccg aagcaatgaa acaagaagta gaagaaataa
     4681 ttgctaatgt agttattaga ggtcgacatc ctaatgaata tgttaaagat atgcgcaagc
     4741 acttaaataa attcgaaggc acagcacgac aaaagaccgc agcaattaaa tcattgcttt
     4801 atacggaatc ggcacgtgtt cacgcacaat caagcattga cagcatgaaa gaaatttcac
     4861 cggaaggata ttatatgtat attgcaaaaa tcgataatag aacaactaaa gtatgcaaag
     4921 ggcttaatgg agaaatattc aaagttaaag acgctaaaat tggtgttaat ttctatccta
     4981 tgcatatcaa ttgtcgttca gattgcgctt tactacctaa atctatgtgg ccgaaaaaac
     5041 caagcaagaa acgaaaaaca aaatacttcg gagggaaagt gaaaagcggt gattgattta
     5101 aaagtgaagt tttttaaagg caagttagtt ttgtatgaca gtaaattaaa tgtttggagg
     5161 atactaatat gagtaatact gacaaatacc ttagagacat agcaagagaa ttaaaaggta
     5221 tacgtaaaga gttacaaaag cgaaacgaaa cagttattat tgatgcaaac ttagacagtt
     5281 taaggtcggc agtattagcc gataaagaaa aatcgaaata taatgaacct ctcttttaat
     5341 agctagcact taattgtgtt ggctgttttt tatgtccaaa acgtgctgat gacataaaaa
     5401 gcacgcatgg aaaaacagtc gacagactat aaatggaggt atatctcatg gaagaaaata
     5461 aacttaagtt taatttgcaa ttttttgcag accaatcaga tgatccggac gaaccaggcg
     5521 gagatggtaa aaaaggaaat cctgataaga aagaaaatga cgaaggtact gaaataactt
     5581 tcacgccaga gcaacaaaag aaagttgatg aaatacttga acgtcgtgta gcccacgaaa
     5641 agaaaaaagc tgatgagtat gcaaaagaaa aagcagcaga agctgctaaa gaagctgcta
     5701 aattagcgaa aatgaacaag gatcaaaaag atgaatatga acgcgaacaa atggaaaaag
     5761 aactggaaca attacgttca gaaaaacaat taaacgaaat gcgttcagaa gcacgaaaaa
     5821 tgttgagtga agcggaagtt gattcatcag atgaggttgt caatttagtt gtaacagata
     5881 ctgctgaaca aactaaattg aatgttgaag ctttttctaa tgcagtaaaa aaagcggtta
     5941 atgaagcggt taaggttaac gctagacaat cgccattgac tggtggagat tcatttaatc
     6001 actcgactaa aaataaaccg caaaacttag ctgaaatagc tagacaaaaa agaattatta
     6061 aaaattaacg gaggcattta aatggaacaa acacaaaaat taaaattaaa tttgcaacat
     6121 tttgcaagta acaatgttaa accacaagta tttaaccctg acaatgtaat gatgcatgaa
     6181 aagaaagatg gcacgttgtt aaacgacttt acaacaccta tcttacaaga ggttatggaa
     6241 aactctaaaa tcatgcaatt aggtaagtac gaaccaatgg aaggtactga gaagaagttt
     6301 actttttggg ctgataaacc aggtgcttac tgggtaggtg aaggtcaaaa aatcgaaacg
     6361 tctaaggcta cttgggttaa tgctacaatg agagcgttta aattaggggt tatcttacca
     6421 gtaacaaaag aattcttgaa ttacacttat tcacaattct ttgaagaaat gaaacctatg
     6481 attgctgaag ctttctataa aaagtttgac gaggcaggta ttttgaatca aggtaacaat
     6541 ccgttcggta aatcaattgc acaatcaatt gaaaaaacta ataaggttat taaaggtgac
     6601 ttcacacaag ataacattat tgatttagag gcattgcttg aagatgacga attagaagca
     6661 aatgcattta tctcaaaaac acaaaacaga agcttgttac gtaaaattgt agatcctgaa
     6721 acgaaagaac gtatttatga ccgtaacagt gattcgttag acggtctacc tgtggttaac
     6781 cttaaatcaa gcaacttaaa acgtggtgaa ttaatcactg gtgacttcga caaattgatt
     6841 tatggtatcc ctcaattaat tgaatacaaa atcgatgaaa ctgcacaatt atctacagtc
     6901 aaaaatgaag atggcacacc tgtaaacttg tttgaacaag acatggtggc attacgtgca
     6961 actatgcatg tagcattgca tatcgctgat gataaagcgt ttgctaagtt agttcctgct
     7021 gatgcaaaac catcttcaaa tccaggagaa gtttaataaa taattaggag tggtaacatg
     7081 cccgaaatca ttggaattgt taaagtagat tttacagatt tagaagataa cagacatgtc
     7141 tatatgaaag ggcatgtcta ccctcgaaaa ggttatgatc ctacagatga acgtatcaaa
     7201 gctttagcta gtgttgaaaa taaacgcaac gaacaaatga tttacattgt aaatgacaaa
     7261 ttaaccaaaa aagaacttgt cgaaatagca agtgttgctg gcttacaagt tgatgaaaaa
     7321 caaacaaaag ctgaaattat caatgctttt gagtcactag agtaggtggt tatatgacta
     7381 cgctagctga tgtaaaaaaa cgtattggtc ttaaagatga aaagcaagat gaacaattag
     7441 aggaaattat aaaaagttgt gaaagccagt tgttatcaat gttacctatt gaagttgaac
     7501 aaataccgga aaggtttagt tacatgatta aagaagttgc agttaaacgc tacaacagga
     7561 ttggtgctga aggtatgaca tcagaagcgg ttgacggacg tagcaatgcg tatgaattga
     7621 acgatttcaa ggagtatgaa gctattattg ataattactt taatgctaga acgagaacta
     7681 aaaaaggaaa ggctgtgttt ttttgagata tgaagataga gccgtctttc aatcagaaca
     7741 agcagcaact tacaatccta aaactagcaa aaaagaaaac gcactaatca cttatgatgc
     7801 gataccatgc aatattaacc ccatttctag agcaagaaag caacttgaat ttggcgatgt
     7861 aaaaaacgat gtaagtgtcc tgaggataaa agaatcaata tcttaccctg ttagccacgt
     7921 gttggttaat ggcattcgct acaagatagt tgatacaagg atatacagac acgaaacgtc
     7981 atattatatc gaagaggtca attgatgaat atagatggat tagacgcact gttaaaccaa
     8041 tttcacgata tgaaaaccaa cattgatgat gatgtagatg atattttaca ggaaaacgcc
     8101 aaagaatatg tagtacgagc taaattgaaa gctagagaag taatgaataa gggttattgg
     8161 actggtaatt tatcacgcaa tatcagatat aaaaaaactg gcgatttgca atacactatc
     8221 acatcgcatg cagcttatag tggtttctta gagtttggta ctcgatacat ggaggcagaa
     8281 ccttttatgt ggccagtata tgaggtaata agaaaatcga ctgtagaaga attgaaagcg
     8341 ttgtttgaat aggagataaa agcatgacac cgaacttaca actttataat aaagcgtatg
     8401 aaacgctaca aggatatgga ttccctgtta tttctcgtaa agagatgcaa caagagattc
     8461 cgtatccttt ttttgtaata aaaatgccgg agtcaaatag aagtaagtac acgtttgata
     8521 gttattctgg cgatacgaat ttagttattg atatttggag tgtaagcgat gatttaggac
     8581 atcatgacgg acttgttaaa aggtgtatcg atgatttaac acctagcgtt aaaacaaacg
     8641 attatgactt tgaagaagat gatactaaca tcgcacagtt agtcgatgat actactaatc
     8701 aagaattgct acacacatca ataacgatat cttacaaaac attttaaaaa acggaggaat
     8761 attgaatggc gaatatgaaa aatagtaatg accgtattat tttgtttaga aaagctggcg
     8821 aaaaagtaga tgctactaaa atgctttttt taactgaata cggcttatca catgaagctg
     8881 atacagatac agaggatacg atggatgggt cttataacac tggtggttca gttgaatcaa
     8941 caatgtctgg tactgctaaa atgttttatg gtgacgattt tgcagatgaa attgaagatg
     9001 cagttgtaga tcgcgtattg tatgaggctt gggaagttga aagtagaata ccaggcaaaa
     9061 atggagatgc tactaaattt aaagcgaaat atttccaagg tttccacaat aaatttgaat
     9121 taaaagcaga agctaacggt attgatgaat atgaatatga atatggagtg aatggtcgtt
     9181 tccaacgtgg atttgcaaca ctacctgagg ctgtaacaaa gaaacttaag gcgactggat
     9241 acagattcca tgacactaca aaagcagatg cgttaactgg cgaagattta acagcaattc
     9301 cacaacctaa ggtagattca tcaacggtta caccaggaga ggtataaaaa tagggcgtta
     9361 agccctattt attttgttta aattaatcat gaatggagat tttaagttat gaatgtagaa
     9421 attaacggaa agtcattaga attaagtttt ggttttaaat ttttaagaga aatcgataac
     9481 cgattaggtt taaaagttga gcaagcttct atcggtcaag gtgtatcaat gttgcctgta
     9541 ggtttagaga gtggaaatcc tgttgtgatt ggcgaagttt taattgcagc tacatctcac
     9601 ttgaaaaaac aagcaattac tattaataac attgatgaag cactagatga aatcgcagaa
     9661 aatattggac tagaagaatt tggttcggat attttaacgg agttgggaaa gcgacctatg
     9721 acccgaaacc tagtcgaagt agtggaaacg gaagagaaac cagcggaagc ctaataactt
     9781 acgacagaat cgttataact tgtatgtcaa cacttggtat tacagatttg aacgttattg
     9841 agcaaatgac attaacagaa tataactatc gaatgtatgc gaaagagtat gaaatgctaa
     9901 cccaagaatt cgaacgttac aaacttgcgt ttgctattcg tgatgctgca gctactaaaa
     9961 atgttgggac agaaaataaa cctaaagagg aatatgtttt taacaacgca aacgacgtat
    10021 tgccttatga agaaaatatc caacggctta acgaaggtaa agatataaga tttagtagcg
    10081 aacgtgatga atacgaacca caaaataatg aattctttaa agttatagca gaatttaaca
    10141 agcaatagaa agagaggtgt taatgtgacg gaatataaaa ttaaagcgac tattgaagct
    10201 agtgtagcca aattcaaaag gcaaatcgat agtgcggtta agtctgtgca aagatttaaa
    10261 cgagtagcag atcaaactaa agatgtcgaa ttaaatgctg atgataaaaa attacaaaaa
    10321 actatcaaag ttgctaaaaa gtctttagat gcctttagta acaaaaaagt aaaagctaaa
    10381 ttagatgcta gtatacaaga cttgcaacaa aaggtactag aatcgaattt tgaactagac
    10441 aaactaaact ctaaagaagt tactccagag attaaattac aaaaacaaaa attgactaaa
    10501 gatatcgctg aagcagaagc taagttatcc gaactagaaa agaagcgtgt caatattgac
    10561 atcaatgcag ataacagtaa attcaatcga gtgttaaaag tatctaaagc tagtcttgaa
    10621 gcattaaata ggtctaaagc caaagctatt atagacgtgg acaacggtgt tgctaactct
    10681 aaaatcaaac gcactaaaga agagcttaaa agtattccaa acaaaactag atctcgacta
    10741 gatgtagata cagggctttc tataccaact atttatgcgt ttaaaaaatc attagacgca
    10801 ttgccgaaca aaaaaacaac aaaggtagat gtcgatacta atggtttaaa gaaagcttat
    10861 gcctacataa taaaagcaaa tgacaatttt caaagacaga tggggaattt agctaatatg
    10921 ttccgtgtgt tcggtactgt aggttctaat atggttggtg gattacttac atcatctttt
    10981 agtatcttaa tacctgtaat agcgagcgta gtacctgtag tatttgcgct attaaacgct
    11041 atcaaagtgt taactggtgg tgtacttgct ttaggtggtg ccgtagcaat agcgggagca
    11101 ggatttgtag cgtttggcgc aatggctatc agcgctataa agatgcttaa tgatggcact
    11161 ttacaagcta gctcagcaac aaacgaatac aaaaaagcgt tagatggcgt aaagtcagca
    11221 tggactgata ttataaagca aaatcaatcc gctatcttca caactcttgc aaatggttta
    11281 aatactgtta aaactgcaat gcagagctta caaccgtttt ttagtggtat ttcaagagga
    11341 atggaagaag cgtctcaaag cgtgcttaaa tgggctgaaa atagcagtgt agcttcaaga
    11401 ttctttaata tgatgaatac aacgggtgtt tcggtattta acaagctatt aagtgctgca
    11461 ggtggttttg gtgacggatt agtcaatgta ttcacgcaat tagcaccact gtttcaatgg
    11521 tcggctgatt ggttggatag attaggtcaa tctttctcta actgggctaa tagtgcagct
    11581 ggagaaaatt cgataactcg ttttattgaa tacacaaaaa caaacttacc tatcattggt
    11641 aatattttca aaaatgtttt cgttggaatt aacaatttga tgaatgcatt cagcggatca
    11701 tcaactggca tattccaatc tcttgaacaa atgacagcta agtttaggga atggtctgaa
    11761 caagtaggac aatctcaagg gtttaaagac tttgtcagtt atatacaaac aaatggacca
    11821 ctaataatgc aattgattgg aaacatcgca agaggattag ttgcattcgc aacagcaatg
    11881 gctcctatag ctagtgcagt attacgcgtt gcagttgcaa taactggttg gatagctaac
    11941 ttgtttgagg cgcatccagc tacagcacaa ttagttggtg tcattataac tttagttggt
    12001 gcatttagat ttttaatacc gattattctt gctgtatcta actttatggg tggcggatta
    12061 ataggtagaa tcattgcatt agtaagtaag ttcggtttat taagagcggg attaacaatt
    12121 ttaaaaggtg cgttcatgtt attaaaagga ccattaaaaa ttatatcagt tatattccaa
    12181 ttgttattcg gtaagattgg attaattaga aatgctatca caggactagt aactgtgttt
    12241 ggtattttag gtggtccaat aacaatagtt attggtgtaa tcgctgcatt aatagctata
    12301 ttcgttttat tgtggaataa aaatgaagga ttcagaaact ttattataaa tgcttggaat
    12361 gcgataaaaa cgtttatggt tacagtttgg aatgtgttga aaactgtagc ttcggttgta
    12421 tggaatgcta tttcaaaagc tatcactaca gcagtaacta atgtatacaa ttttataatg
    12481 attgtttgga atcaaatagt cgcttattta caagggttat ggaatggaat tatcgctatt
    12541 gcaacaacgg tgtggaacct tttagttaca atcatcacaa ctgttttcac gacgataatg
    12601 acaatagtta tgacgatatg gacagctatt tggacattct taagtacaat ctggaacacg
    12661 ataattacaa tcgctactac gatttggaat ttgttagtca ctgtaataac tacagtgttt
    12721 accacaatta tgactatcgc aatgacaatt tggaacgcta tttggacgtt cttacaaacg
    12781 ttgtggaaca ctatagttac tgtggcaact aaggtttgga acgctatcac tacagctata
    12841 tctactgcgt tacaagcggc atggagtttt atttctaata tatggaatac gatttggagt
    12901 ttcttatctg gtatattaac gacaatttgg aataaagttg taagcatatt cacacaagtt
    12961 gtttcaacta tatcagacaa aatgtctcaa gcttggaact tcattgtcac taaaggtatg
    13021 caatgggtat ctactataac aagtacgcta attaactttg ttaatagagt tattcaagga
    13081 ttcgttaatg ttgtaaacaa agttagtcaa ggtatgacaa atgcagtaaa taaaataaaa
    13141 agctttatag gagattttgt gtctgcaggt gctgatatga tccgtggttt aattagaggt
    13201 attggacaaa tggctggtca attagtagat gcagctaaaa atgttgctaa gaaagcttta
    13261 gatgcagcta aaagtgcttt gggtattcac tcaccttcac gtgaattcat ggatgttggt
    13321 atgtattcaa tgctaggttt cgttaaaggt atagataatc attcaagtaa agttatccgt
    13381 aatgtttcta atgttgcaga taaagtagtt gatgcatttc aacctacatt aaacgcacct
    13441 gacatttcta gtattacagg aaacttaagt aatttaggtg gaaatataaa tgcgcaagta
    13501 caacacacac attctattga aacatcaccg aacatgaaaa ctgttaaaat tgaattcgat
    13561 gtcaataacg atgcgcttac tagtattgtt aacggcagaa atgctaaacg caattctgag
    13621 tattacttat aaaggaggtt acaaatggac atagaattaa caaaaaaaga tggtactgta
    13681 atcaaattaa gtgaatacgg gtttatcgtt aacgatatag taattgatag catgcaaatc
    13741 aacacaaagt atcaagacaa agaaaatatg aacggtcgca tattaatggg gagcaattat
    13801 atcagtagag atatagttgt tccttgtttt tgtaaagtaa aaaatcgttc agacattgct
    13861 tatatgcgag atatgttgta ttcgttaact actgatatag aaccaatgta tttacgtgaa
    13921 atcagaagaa aagaagagtt gaattacagg tttactcaac caacttctga tgattacgtg
    13981 aaattagata aaaacaactt cccggattat gaatattcaa gacacgatca acaaatttat
    14041 gtaaacggta aacagtataa agttattttt agcggagtta taaatcctaa acaaaaaggt
    14101 aacaaagttg ctttcgaatt gaagtttgaa actacagaat taccatacgg cgaaagtatt
    14161 ggaacaagcc tagagttaga agaaaacaaa aaggttggat tgtggtcgtt tgattttaat
    14221 attgattggc atgcaggtgg ggataagcgc cagtatacat ttgaaaatgt tagcaaagat
    14281 acagtttact atcatggtag tgctcctaac gaccaattca acatgtataa aaagataaca
    14341 attattttgg gcgaagatac agaatcgttt gtatggaatt taacgcatgc tgaaataatg
    14401 aaaatcgaag gtatcaaact aaaagctgga gacaaaattg tttatgatag cttccgagtt
    14461 tataaaaacg gtgttgaaat aagtaccgaa acgaacatag cccaaccaaa atttaaatac
    14521 ggagctaata aattcgaatt taaccaaaca gttcaaaagg ttcagttcga tttgaaattt
    14581 tattataagt aggtgtcaga atgacgataa ctgttaaacc acctaaaggc aatggcgcac
    14641 ccataccagt agaaacaact ttagtgaaaa aagttaatgc tgacggtgta ttaacttttg
    14701 atattctcga aaacaaatac acttatgaag ttattaacgc tatagggaaa agatggattg
    14761 ttagtcatgt cgaaggtgaa aatgacaaga aagaatatgt aataactgtc attgatagga
    14821 aatcagaagg cgacagacaa ctggttgaat gtactgctag agagattcct atagacaagt
    14881 taatgattga taggatttat gttaatgtaa caggatcttt tacagtagaa agatatttta
    14941 acattgtgtt tcaaggtact ggaatgcttt ttgaagtcga aggtaaggtt aagtcttcga
    15001 agtttgaaaa tggtggtgaa ggcgacacaa ggttagaaat gtttaaaaag gggttagaac
    15061 atttcggttt agaatataaa ataacgtatg acaaaaagaa agacagatat aagtttgtat
    15121 tgacgccttt tgcaaatcaa aaagcgtctt attttatttc tgatgaagtc aacgccaacg
    15181 ctataaaact cgaggaagat gcaagtgatt tcgccacctt cattagagga tatggtaatt
    15241 attcaggaga agaaacattc gaacacgctg ggctcgtaat ggaagctaga agtgcattag
    15301 ctgaaatata cggcgacatc cacgcagaac catttaaaga tggtaaagtg actgaccaag
    15361 aaactatgga taaagaatta caatcgagat tgaaaaagtc gttaaaacaa tctttgtctt
    15421 tggacttttt ggtgttaaga gaatcatatc cagaagcaga cccacaaccc ggagacatag
    15481 tacaaataaa atctaccaaa ctaggtttga atgatttagt ccgtatagta caagttaaaa
    15541 cgattagggg tataaacaat gtaattgtta agcaagatgt aacgcttggt gagtttaatc
    15601 gagaacaacg atatatgaaa aaagttaata ctgcagctaa ctatgtttct ggattaaatg
    15661 atgttaacct ttctaatcct agtaaagcgg cagaaaactt gaagtctaaa gtagcgtcaa
    15721 tagctaaatc aacactcgat ttgatgagta gaactgatct aattgaagac aaacaacaga
    15781 aagtgagttc taagactgta actacatcag atggcactat cgttcatgat tttatagata
    15841 aatcaaacat taaagatgta aaaacaattg gaacgattgg cgattctgta gctagaggat
    15901 cacatgcgaa aactaatttc acagaaatgt taggcaagaa gttaaaagct aaaacgacca
    15961 accttgcaag aggtggcgca acaatggcaa cagttccaat aggtaaagaa gcggtagaaa
    16021 acagcattta tagacaagca gagcaaataa gaggagacct aatcatatta caaggtacag
    16081 atgatgactg gttacatggt tattgggcag gcgtaccgat aggcactgat aaaaccgaca
    16141 ctaaaacgtt ttacggcgcc ttttgttctg caattgaagt tatcaggaaa aataatccag
    16201 cttcaaaaat acttgtaatg acagctacta ggcaatgccc tatgagtggt acaacgatac
    16261 gccgtaaaga tacggacaaa aacaaactag ggttaacttt agaggattat gtcaatgctc
    16321 agatattggc ttgtagtgaa ttggatgtac cagtatatga tgcctatcat acagattatt
    16381 ttaagccata taatccagcg ttcagaaaat caagtatgcc agacggattg catccgaacg
    16441 agaggggtca cgaagttatt atgtacgaac ttattaaaaa ttattaccag ttttacggat
    16501 agaaaaggag gaagacatgg ataacaaatt aattacagac ttaagtagag tctttgacta
    16561 cagatatgta gatgaaaatg agtataactt taaacttatt tcagacatgc tgacggattt
    16621 taatttctct cttgaatacc atagaaataa agaggtattt gcacataatg gagagcaaat
    16681 aaagtatgag catttaaatg tcacaagtag cgtctctgat tttttaacgt atctaaacgg
    16741 ccgtttcagc aatatggtac taggtcataa cggcgacggt atcaacgaag taaaagacgc
    16801 gcgtgttgat aatactggtt atgatcataa gacattgcaa gatcgtttgt atcatgatta
    16861 ttcaacacta gatactttca ctaaaaaggt tgagaaagct gtagatgaac actataaaga
    16921 atatcaagcg acagaatacc gatttgaacc aaaagagcaa gaaccggaat tcatcacaga
    16981 tttatcgcca tatactaacg cagtaatgca atcattttgg atagacccta gaacgaaaat
    17041 tatttatatg acacaagcgc gtccgggcaa tcattacatg ttatctagat tgaagcctaa
    17101 tggacaattt attgatagat tgcttgttaa aaacggcggt cacggtacac acaatgcgta
    17161 tagatacatt gatggagaat tatggattta ttcagctgta ttggacagta acaaaaacaa
    17221 caagtttgta cgtttccaat atagaactgg agaaataact tatggtaatg aaatgcaaga
    17281 cgtcatgccg aatatattta acgacagata tacgtcagcg atttataatc ctatagaaaa
    17341 tttaatgatt ttcagacgtg aatataaagc ttctgaaaga caagctaaga attcattgaa
    17401 tttcattgaa gtaagaagtg ctgacgatat tgataaaggt atagacaaag tattgtatca
    17461 aatggatata cctatggaat acacttcaga tacacaacct atgcaaggta tcacttatga
    17521 tgcaggtatc ttatattggt atacaggtga ttcgaataca gccaacccta attacttaca
    17581 aggcttcgat gtcaaaacaa aagagttatt atttaaacgt cgtatcgata taggcggtgt
    17641 gaataacaac tttaaaggag atttccaaga ggctgagggt ctagatatgt attatgatct
    17701 agaaacagga cgtaaagcac ttttaattgg ggtaactatt ggaccaggta acaacagaca
    17761 tcactcaatt tattctatcg gtcaaagagg tgtaaaccaa ttcttaaaaa acattgcacc
    17821 tcaagtatca atgactgatt caggcggacg tgttaaaccg ttaccgatac agaacccagc
    17881 atatctaagt gatattacgg aagttggtca ttactatatc tatacgcaag acacacaaaa
    17941 tgcgttagat ttcccgttac cgaaagcgtt tagagatgca ggttggttct ttgatgtact
    18001 gcctggtcat tataatggtg cgttaagaca agtactaact agaaacagca caggtagaaa
    18061 tatgctcaaa tttgaacgtg ttatcgacat ctttaacaag aaaaacaacg gttcatggaa
    18121 ttttaaccca caaagtgctg gttattggga acatatccct aagagcatca cgaaattgtc
    18181 tgatttaaaa attgttggtt tagacttcta tatcaccact gaagaatcaa aacgtttttc
    18241 tgacttccct aaagattaca aaggtattgc aggctgggtg ttagaagtaa aatcaaatac
    18301 accgggtaac acaacacaag tgctaagacg taataacttt gcttctgctc accagttttt
    18361 cgttagaaac tttggtactg gtggtaatag tggttggagc ataatagaag gtaaggaggt
    18421 tgaataatgg tagtagataa tttttcaaaa gatgataact taatcgagtt aaaaacaaca
    18481 tcacaatata atccagttat tgacacaaac atcagtttct atgaatcaga tagaggaact
    18541 ggtgttttaa attttgcagt aactaagaat aacagaccgt tatctataag ttctgaacat
    18601 gttaaaactt ccatcgtgtt aaaaaccgat gattataacg tagatagagg cgcttatatt
    18661 tcagacgaat taacggtagt agacgcaatt aatgggcgct tgcaatatgt gattccaaat
    18721 gaatttttaa aacattcagg taaggtacat gctcaggcat tcttcacgca acacgggagt
    18781 aacaacgtag ttgttgaacg tcaatttagt ttcaatattg aaaatgattt agtcagtggg
    18841 tttgatggca taacaaagct tgtttatatc aaatctattc aagatactat cgaagctgtc
    18901 ggtaaagact ttaaccaatt aaagcaaaat atggctgata cacaaacgtt aatagcaaaa
    18961 gtgaatgata gtgcgacaaa aggcattcaa caaatcgaaa tcaagcaaaa cgaagctata
    19021 caagctatta ctgcgactca aactagtgca acacaagctg ttacagctga attcaataaa
    19081 atagttgaaa aggagcaaac gatatttgcg cgtgtcaatg aagttgagca acaaatcaat
    19141 ggtgctgacc ttgtcaaagg caactcgaca gtcaattggc aaaagtctaa gattactgat
    19201 gattatggca aagcgattga atcgtctgag cagtccatag atagcgtttt aagcacagtt
    19261 aacacatcta ggattattca tattactaac gcaacagatg cgccagaaaa gacggatata
    19321 ggcacgttag agaagcctgg acaagatggt gttgatgacg gttcttcgtt cgatgaatca
    19381 acttatacat caagcaaatc tggtgtgtta gttgtttatg ttgttgataa taatactgct
    19441 cgtgcaacat ggtacccaga tgattcaaac gatgagtaca caaaatacaa aatctacggc
    19501 acgtggtacc cgttttataa aaagaatgat ggaaacttaa ctaagcaatt tgttgaagaa
    19561 atatctaaca acgctttaaa tcaagctaaa cagtatgtag atggtaagtt tcaaagtaca
    19621 agttggcaac aacataaatt aacagaacat aacggtcaat caatccaaaa gaacttatat
    19681 aacgccaaag gtaatttaga agcattgggc gctgggaatt attacgtaac aagtgtacct
    19741 gatttaccag gtattgttga aagttacgaa ggctacttat cagtatttgt taaagatgat
    19801 gcaaataagt tattcaactt cacaccttca aactctaaaa aagtttatac acgatcaatc
    19861 accaatggtc gattagattc acaatgggct gcacctaacg aacataaaac agccgtgtta
    19921 ttcgacggtg ctgcaaacgg tgtaggaaca aggattaatt taaccgaagc ttatacaaat
    19981 tatgcaattc tattcataag cggtacttat ccaggtggtg ttattgaagc attcagttta
    20041 acctctatac caaatgcgat tcaattaagt aaaacaaatg tagttgactc agacggtaac
    20101 ggtggtggta gttatgaatg cttaataact aaagaaagtg gtacgacgtt aaaaatcgat
    20161 aacgatgtgt accttgattt aggcagtaaa acaggttctg gtgctaatgc aaacagagtt
    20221 acgataaata aaattgtggg gtggaaataa tgaaaatcac agtaaacgat aaaaacgaag
    20281 ttatcggata cgttaatact ggcggattac gcaatagttt agatgtagat gataacaatg
    20341 tgcctattaa atttaaagaa gagttcgaac ctagaaagtt tgttttcact aacggcgaaa
    20401 ttaaatacaa tagcaatttc gaaaaagaag acgtaccgaa tgcatcaaaa caacaaagtg
    20461 aatcagattt gagtgatgaa gaacttcgcg gaatggttgc aagtatgcaa atgcagatga
    20521 cgcaagtgaa catgttgaca atgcaattga cgcaacaaaa cgctatgtta acacaacagt
    20581 tgaccgaact gaaaactaac aaaacaaata ctgaggggga cgtttaaatg atgaagatga
    20641 tttatccaac ttttaaagac attaaaactt tttatgtgtg gggttgctat aaaaatgagc
    20701 aaattaagtg gtacgtagac atgggtgtaa tcgacaaaga agaatatgca ttgatcactg
    20761 gagaaaaata tccagaaaca aaagatgaaa agtcacaggt gtaatgcttg tggcttttta
    20821 atttaacaaa aagtaggtgg cgtaatgttt ggttttacca aacgaca
//
LOCUS       DANRDI010000033        20845 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.033, whole genome shotgun sequence.
ACCESSION   DANRDI010000033 DANRDI010000000
VERSION     DANRDI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.033"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(87..932)
                     /locus_tag="PZ377_000956"
     CDS             complement(87..932)
                     /locus_tag="PZ377_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDM6357841.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    1161..1530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1596..2699
                     /locus_tag="PZ377_000957"
     CDS             1596..2699
                     /locus_tag="PZ377_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDM6357842.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            2696..3856
                     /locus_tag="PZ377_000958"
     CDS             2696..3856
                     /locus_tag="PZ377_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDM6357843.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            3825..5666
                     /locus_tag="PZ377_000959"
     CDS             3825..5666
                     /locus_tag="PZ377_000959"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDM6357844.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            5663..7891
                     /gene="metE"
                     /locus_tag="PZ377_000960"
     CDS             5663..7891
                     /gene="metE"
                     /locus_tag="PZ377_000960"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDM6357845.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            7934..8686
                     /locus_tag="PZ377_000961"
     CDS             7934..8686
                     /locus_tag="PZ377_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDM6357846.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(8769..9950)
                     /locus_tag="PZ377_000962"
     CDS             complement(8769..9950)
                     /locus_tag="PZ377_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDM6357847.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            10489..11622
                     /locus_tag="PZ377_000963"
     CDS             10489..11622
                     /locus_tag="PZ377_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDM6357848.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(11989..12618)
                     /locus_tag="PZ377_000964"
     CDS             complement(11989..12618)
                     /locus_tag="PZ377_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDM6357849.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(12618..13460)
                     /locus_tag="PZ377_000965"
     CDS             complement(12618..13460)
                     /locus_tag="PZ377_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6357850.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(13485..14198)
                     /locus_tag="PZ377_000966"
     CDS             complement(13485..14198)
                     /locus_tag="PZ377_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDM6357851.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(14195..14398)
                     /locus_tag="PZ377_000967"
     CDS             complement(14195..14398)
                     /locus_tag="PZ377_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6357852.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            14639..15028
                     /locus_tag="PZ377_000968"
     CDS             14639..15028
                     /locus_tag="PZ377_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDM6357853.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            15128..15352
                     /locus_tag="PZ377_000969"
     CDS             15128..15352
                     /locus_tag="PZ377_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDM6357854.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            15369..16070
                     /gene="tatC"
                     /locus_tag="PZ377_000970"
     CDS             15369..16070
                     /gene="tatC"
                     /locus_tag="PZ377_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDM6357855.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(16162..17874)
                     /locus_tag="PZ377_000971"
     CDS             complement(16162..17874)
                     /locus_tag="PZ377_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDM6357856.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(17855..19084)
                     /gene="efeB"
                     /locus_tag="PZ377_000972"
     CDS             complement(17855..19084)
                     /gene="efeB"
                     /locus_tag="PZ377_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDM6357857.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(19081..19935)
                     /locus_tag="PZ377_000973"
     CDS             complement(19081..19935)
                     /locus_tag="PZ377_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357858.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(20203..20748)
                     /locus_tag="PZ377_000974"
     CDS             complement(20203..20748)
                     /locus_tag="PZ377_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357859.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
BASE COUNT         6737 a         4033 c         3088 g         6987 t
ORIGIN      
        1 aaagtctcaa tatatctaga atcaaatgtt cactgattac aatttattta cacttgcaca
       61 aagagttatt atataaaatt gtgtaactat ttaccatacc tacgatttaa ttgttcaatt
      121 attctcacaa aatcttcttg tgaatcaaac tcaaatgaga ttttaccaac cgattttttt
      181 attgatatat ctactttggt accatactgt tctcgcaact gtcgttcttg ctgctttata
      241 aatttaggct tagttatatc tactttgtct gtttccgact ttgacgaaac attttttaat
      301 tcattaacat ggttttctaa atatctgaca ctccactttt ctttaacaac ccgtttcgct
      361 aacctaagca tttgttgttc atctttaatt gccaataacg ttcgtccatg tgcacttgtc
      421 agtcgcccat cttttaccat gtcagcaatc tttttcggca aatgtaataa cctcaacata
      481 ttcgctatat acgggcgcga cttactcaat cgtttcgcta cttcttgttg tgtaattttc
      541 aaatctgtca tcaaacgttg ataactttca gcttcttcaa tcgcatttaa gtcttctcgt
      601 tgtaaatttt cgatgaccgc cagttccatc atatcttcat ctgttaaatc tttgataatc
      661 gctgatacgt attttagacc agcaattttc gaagctctaa accttctttc accaactaca
      721 atgtaataac cttgaactgt ttttctaagc acaattggtt gcaaaattcc atattgctta
      781 attgaatctg ccaaatcatt taaatgattt tcatcgaaag tttttcgggg ctgatacgga
      841 ttcggtttga tatccgatat atctatttgc ttaatttgtt catcttttgt tttagtaata
      901 cgttgatctt cactttttga caattcactc acatcacttt ttatttgata acaacagtta
      961 aatatatttt atgtattcta aatgagtcag acaaccatta accacacttt atttaaaaaa
     1021 taattcattt ttcaacttca agataccaat tacaatttca caaacaaatt aacttttaaa
     1081 tcaaaatttt ctgaataatt gttgacaaat ctttttttac tctgtaagct aattaatatc
     1141 ttataacagt ttaatgaaac gtaaacacaa taaagaggaa agtaaaacac accctgctta
     1201 tacagagagt ctttagtagc tgagagaaga ttttgaaagc gtgtttgaaa atggccttgg
     1261 agtgttgatg ccaatatgag gtgtctacgg gttcgcccgt tatagcgata cagtattaac
     1321 attgatgtta aatggcgtac tggattcttt acgcacgatt ttttgttaat aagtatggga
     1381 tagcacatta ctatatcctt acttactgac tttaattgtg ataattgttc agtaagcata
     1441 tttactttta atgcgtactg aataaggtta tttcagcgat ggaataacaa ataaaggtgg
     1501 taccgcgaaa cataagcttt cgtccttttt atccgattca ttcgggtacg aaggacggaa
     1561 gcttttttta ttttttctta taggagggtc ttaatatgaa ggatacacag ttagcccaaa
     1621 tcacattaac cgatgattca accggtgcta tagcgaatcc aatccattta tctactgcct
     1681 acaagcatcc aaaactagga caatcgacag gttttgatta tacacgtact aaaaatccta
     1741 cacgctcaac atttgaaacc tgttttgcca aacttgagca tggtattgca tcattcgcta
     1801 catcaagtgg aatgtcagcc attcaattaa tatgtaatct atttaaacct catgatgaaa
     1861 ttttagtttc attcgattta tatggtggca catttagatt atttgaattt tacgagcaac
     1921 aatacgatat caaatttaag tacgttgatt ttacagatta tgaacaagtt gaaaaagaaa
     1981 tcactgataa aacagttgca ttattcattg aaccaatatc taacccacaa atgattgcta
     2041 ttgatgtaaa gccatactat caactttgta aagctaaagg cttattgtca attatcgaca
     2101 atactttttt aacaccttat ctttcaacac cactagcaga aggtgctgat atagtcttac
     2161 attcagccac gaaatatatt ggcggacata acgatgtact agcaggtgtc gtaaccgtca
     2221 aagatgaatc actcgcgcaa cagttgtttg attttcacaa catgactggc gcaacacttt
     2281 caccaataga tagttatttg ttgttacgtg gacttaaaac tttgcattta cgcattgagc
     2341 gtgcgcaatc aaacgctaga aaacttgcta aaaaatgtca gtcacttcaa gcaattgacg
     2401 aagtactata tagcgggcaa actggcatgc ttagtttaag acttaacaag gcctatagcg
     2461 tcgctaaatt attagaaaat ttagacattt gcatttttgc agaaagttta ggaggtactg
     2521 aaacattagt gaccttccct tacacccaaa cacatgttga tatgccagat gctgaaaaag
     2581 ataaacgtgg cattgatgag tatttaatcc gcttatcact aggtgttgaa aattatgaag
     2641 acatcgaacg cgatatcatc caagcattag ataaagctca gattggagag attgtatgac
     2701 actttcaaaa gagacagaag tgatattcga ttggcgtaga ggtgtggaat atcattcagc
     2761 taacccacca ctctatgatt cttcaacatt ccatcaaaca agtcttggtg gcgatgttaa
     2821 atatgattat gcacgaagtg gcaaccctaa ccgtgaactt ttagaagaga agttagcacg
     2881 attagaacag ggaaaattcg cttttgcttt tgcatcaggt attgctgcta tttcagcagt
     2941 acttttgact ttcaaatctg gtgatcatgt catcttaccc gatgatgtat atggcggtac
     3001 ttttcgcctc actgagcaaa ttttgaatcg atttaatatt gaatttacaa ccgtagatac
     3061 tacaaagctc gaacaaatcg agggtgccat tcaatcaaac acaaaattaa tttatatcga
     3121 aacaccatcg aatccctgtt ttaaaattac tgatatcaaa gctgtttcta aaatagccga
     3181 aaagcatgaa ctactggtag ctgtggacaa tacatttatg acaccgttag gtcaatcacc
     3241 tttattactt ggcgctgata ttgtcattca tagtgctaca aaatttttaa gtggacatag
     3301 cgatttaatt gctggtgctg tcataactaa taatgaggca attagtgacg ctctttattt
     3361 aatacaaaat ggtacaggca atatgttatc tgctcaggat agctggacac ttgctaaaca
     3421 tttaaaaact tttccaatca gatttaaaca atctgtcgaa aacgctcaaa aaatcgtgtc
     3481 atttttaata aagcaagatg aaatttcaga agtttattat ccgggactca ctactgctca
     3541 tttagaacaa gctaaaaatg gcggtgccgt tattggcttt cgtttagctg atgagtctaa
     3601 agcacaacaa tttgtcgatg cactgacatt accactcgtt tcagtgagtc tgggcggtgt
     3661 tgaaacgatc ctttcacatc cagcaacaat gtctcacgct gcactacctg aagaagtgag
     3721 acaagaacgt ggtatcactt tcggtctatt ccgattaagt gttggtctcg aagatcctga
     3781 tgaactcatt gcagacatca aatacgcatt aaaggaggca ttcaatgagt caattcctca
     3841 cacaattgaa agataatgtt ttagtagctg atggcgctat aggaaccatt ttatactctg
     3901 aaggattaga cacctgtcca gaagcatata atcttagcca tccagataaa gttgaacgca
     3961 tccatcgttc atatattgaa gccggtgctg atgtcattca aaccaatact tatggtgcaa
     4021 attttgaaaa gttaaaacga ttcggtcttg aagataaagt taaagcaata catcaagccg
     4081 ccgttcgcat cgcaaaaaaa gcagcaaata aagatacgta tatattaggc acagttggtg
     4141 ggtttagagg tatcaaacaa gaggatatca gcttacaaac tattctttat catactgaaa
     4201 ttcaaataga caccttaatt gaagaaggcg ttgacgcgct acttttcgaa acgtattacg
     4261 acctagaaga gttaacaaat gtcatttcac gaacgagaaa gaaatacgac attccaatca
     4321 ttgctcaatt aaccgcttca aacacaaatt acttagttaa tggtcaggca atcaatgaag
     4381 gattaaaaca actcgttcaa tgtggtgcaa acatcgtggg actcaattgt catcatggcc
     4441 cgcaccatat gcaagagtct ttcacacata ttgaattacc agagcacgca ttcttatctt
     4501 gttatccaaa tgccagctta ttagatattg aaaatagtga atttaagtat agtgacaatg
     4561 cacaatattt cggtcaagtt gctcaaaatc taattcgcga aggtgttcgt ttaattggtg
     4621 gttgctgtgg tacaacgcca gagcacatca aatttattaa agaatctatt cagacactta
     4681 aacctgttaa tgacaaaaaa gtgattccga taccaacgaa agcacttttc aatccatctc
     4741 aaaataaagt tagacaatca ttaacatcta aggttcaaga acgtccaacc gttattatcg
     4801 aattggatac accgaaacat ttagacacgg atagattttt tgaaaatatc gctaaacttg
     4861 ataaagctaa tgtagatgcg gtaacactcg cagataattc attggcaact gtcagaatta
     4921 gcaatattgc tgctgctagc ttaattaagc aatattacaa tattgaacca ctcgtacata
     4981 ttacatgtcg agaccgaaac ttaatcggct tgcagtccca tttacttgga ttatcgctca
     5041 ttggcgttaa cgaaatatta gccataactg gtgatccttc aaaagttggt cacttaccag
     5101 gtgcaaccaa tgtctatgat gttaattcta aaggattaac tgaactcgct ctaagattta
     5161 atcaaggtat taacactgac ggtgatgcgc tgaagaaacg tacacacttc aacatcgctg
     5221 gcgcctttaa ccctaatgtt cgtaaattat atggtgccgt caaaagatta gagaaaaaga
     5281 tagaaagcgg aatgtcttat tttataacac aacccgtgta cagcaaagag aaaatcattg
     5341 aaatttacca tgccactaag cacttgaaca aaccattttt cataggcatt atgcctatcg
     5401 caagttacaa aaacgcactc tttttgcata atgaagtgcc aggaatcaag atgtcagatg
     5461 aaattttaca acaatttgaa gcagttaaag atgataaagc caaaacacga gaactaagtc
     5521 ttaagctttc aaaggattta atcgatactg ttcatgaata ttttaatggt ttatacatta
     5581 tcacaccgtt tcaaaatgtc gaagattcat tagaacttgc agcatactca aaatctatta
     5641 ctgctcacaa ggaggcaata ttatgacaac aattaaaaca tcaaacttag gattcccaag
     5701 attaggtaga aaaagagaat ggaaaaaagc catcgaaagt tattgggcaa agaagatttc
     5761 taaagaagaa ttagatcaaa cattaactga tctacataaa gaaaatttat tattacaaaa
     5821 gtactatcac ttagatagca tcccagttgg tgatttctct ttatatgacc atatattaga
     5881 tacgtcatta ttattcaaca tcatccctga acgtttccaa ggaagaacta ttgatgatga
     5941 tttattattt gatattgctc gcggtaataa agaccatgtt gcaagtgcac ttataaaatg
     6001 gtttaacact aactatcatt acatcgtacc tgaatgggac aatgttgagc ctaaagtgag
     6061 tcgcaatgta ttattagatc gctttaaata tgcccaatct ttaaacgtta acgctcaccc
     6121 tgtcattgtt ggtccgatta cttttgttaa attatcaaaa ggcggtcacc aaacttttga
     6181 agaaaaagtt aaaacgttat taccacttta taaagaagtg tttgaatcat taattgatgc
     6241 aggtgctgag tacattcaag ttgatgagcc aatcttagtt acagacgaca gcgaaagcta
     6301 tgaaaatatt acacgtgaag cttatgacta tttcgaaaaa gctggtgttg ctaaaaaatt
     6361 agtcattcaa acatactttg aacgtgcaca tcttaaattc ttaagttctt tacctgttgg
     6421 tggtataggt ttagactttg tccatgataa tggctataac cttaaacaaa ttgaagctgg
     6481 agattttgat aaatcaaaaa cattatacgc tggaattatt gatggtcgta atgtatgggc
     6541 aagtgacatt gaagctaaaa aagtcttaat cgataaattg ttagcacaca ctaatgaact
     6601 tgtcattcaa ccatcatctt cattattaca tgttccagta tctttagatg atgaaacatt
     6661 agatacaagt gttggcgaag gcttaagctt tgcaactgaa aaattagacg aattagatgc
     6721 attgcgtcgc ctatttaatc aaaacgatag tgttaaatat gataaattaa aagcacgtta
     6781 tgagcgtttc caaaatcaat cattcaaaaa cttagattat gatttcgaaa gcgtgcgtac
     6841 ttcaagacaa tcaccattcg cgcaacgtat tgaacaacaa caaaaacgtt tgaacttacc
     6901 ggatttacca acaacaacta ttggatcatt cccacaaagc cgagaagttc gaaaataccg
     6961 tgcagattgg aagaacaaac gcattacaga cgaagcatat gaaacattct taaaaaatga
     7021 aattgctcga tggattaaaa ttcaagaaga cattggctta gatgtattag ttcacggtga
     7081 atttgaacgt aatgacatgg ttgaattctt cggagaaaaa ttacaaggtt tcttagtaac
     7141 taaattcggt tgggtgcaat catatggttc acgtgccgta aaaccaccaa tcatttatgg
     7201 tgatgtaaaa tggacagcgc ctttaactgt tgatgaaaca gtttatgcac aaagcttaac
     7261 agataaacca gttaaaggta tgttaactgg acctgtaaca attctaaact ggtcatttga
     7321 acgtgttgat ttaccacgta aagtcgttca agatcaaatt gctttagcaa tcaacgaaga
     7381 agtattagca cttgaagctg caggaatcaa agttatccaa gttgacgaac ctgcattacg
     7441 tgaaggctta ccattacgct ctgaatatca cgaacaatat cttaaagatg ctgttttatc
     7501 atttaaactt gcaacgtctt cagttcgtga tgaaactcaa atccatacac atatgtgtta
     7561 ttctcaattc ggtcaaatca ttcatgctat tcatgactta gatgctgatg ttatttcaat
     7621 tgaaacatct cgtagccatg gtgatttaat taaagacttt gaagatatta attatgattt
     7681 aggtattggt ctaggtgtat atgatattca tagtccacgt attccaacaa aagaagaaat
     7741 tactacagca atcaatcgtt cactacaaca aatcgaccgc tcattattct gggtaaaccc
     7801 tgactgtggt ttaaaaacgc gaaaagaaga agaagttaaa gatgcattga ctgtgcttgt
     7861 gaatgctgtt aaagctaaac gccaagaata atcgcagcat ttatcatata ataaactaaa
     7921 aacgggtgat tttatgacac aatatccttt atggcaacaa ttaaatactt taaaacaagc
     7981 aacttgggtt gatttaacgc atacatttga tccggaaata ccacgtttta gtgagtttga
     8041 aaaaggcgaa gtttcaactt tattcacggt taaagatcat ggtttctatg tgcaacgttg
     8101 gaatattgta acgcaatatg gtactcacat tgatgcacca attcactttg tcgagaataa
     8161 acgttattta gaagacattg atttaaaaga attggtattg ccgttaattg tattagactt
     8221 ttcaacagaa gtagcgaaca acaatgattt catcgtaaca cgtgcgcata tagaagcatg
     8281 ggaaaaagag catggtacta ttgaacctgg cacttttgtt gcacttcgta ccgattggtc
     8341 aaaacgttgg cctaatattg aaaagtttga aaacaaagat gcgaacggac aacaacatgc
     8401 accgggttgg ggattagatg ctttgaagta cttaattgaa gaacgtcgtg ttgaagctgt
     8461 tggtcatgaa acattcgata ccgatgcatc tgtggacgtt gtaaaaaatg gtgatttagt
     8521 tggcgaacgt tatattcttg gtcaggataa ataccaagtc gagttattaa ctaatttaga
     8581 tcaattacca actcgtggcg ccatcattta cgctatcagc cctaaaccaa aagatgcacc
     8641 tgggttccct gttagagcat ttgcaattaa accatcaaat gactaatatt acccaataaa
     8701 tccataaaca aataaaaaca ggttatgcca acatgtaatt aatactgttg tgataacctg
     8761 ttttcttatt atttatactt tgatacaact gcagcgatag cttgaccgcc accaatacac
     8821 aatgatgtta aaccagtttc aacttcatca ttcagttgat gcaataatgt cactaatact
     8881 ctagcaccag atgcaccaat aggatgtcct aatgcaatag cgccaccttt aacattcacc
     8941 ttttcaggag gtaattttaa ttcacgatca acagctaatg attgtgctgc aaatgcttca
     9001 tttaattcaa atacatcaat atcgcttaat tcttttttac tacgtttcaa agccttttct
     9061 acagcgccaa ctggtgcaat acccataata gaaggatcta caccatgact tccaaagcca
     9121 tcaagcactg ccaatggttc gatatttaat tctttagctt tgtcttctga catgactaac
     9181 atcatcgcag caccatcatt gattcctgat gcattacctg ctgtaactgt accgtctttt
     9241 ttgaaagctg gtcttaatcg acttaatttt tcgactgata cattttcacg tacaccttca
     9301 tccttagtga ctacgattgg ttcaccttta cgttgaggaa tcgatactgg aactatttca
     9361 ctatcaaatt caccattttg ctgtgcacgt actgcttttt gttgtgagtt tacagcaaat
     9421 gtatcttgtt cttctcttga aataccatat tgctctacta aattttcagc agtaataccc
     9481 atatgatatt gattaaatac atctgttaaa ccatcatata ccatgctatc aaccattgat
     9541 tgatgtccca ttttaaaacc aaagcgactg ttgttgacaa gcattggtga ttgagacata
     9601 ttctccatac cgccagctag cacgatgtca ttttcaccag tcacaataga ttgatatgct
     9661 aattgaatcg actttaaccc agaaccacat actttattca ccgtaaatgc aggtactgtt
     9721 tctggcaagc cacctttcat agcagcaatt cgtgctggat tttgtccttg tcctgcttgt
     9781 agtacgttac cgatgataac ttcatcaatc tcacttggat tcaaacccgt ctctttaata
     9841 atatgttcta ttaaagtcgc acctaaatca taggctggca cgtctttaaa cgcacctcca
     9901 aaaacgccaa taggtgtcct gtatgctgct gctaatacga ctctcgtcat gtcatattca
     9961 tctccttatg atatatacaa ttttattact aatacgtcta atgaaccaat acgccactaa
    10021 catccaattt atgaaaccaa ttgctacgta tgttacattt tcattcttgt tatgcattta
    10081 acaaatcata ttatacaatg atgtaatttg tttgggcaat cggaaacgta aacggtttca
    10141 ctttaacatt tcaccctctt aaatatgctc tatcatcctc atctatagta caaaattatg
    10201 ctttgattta ccacaaatta tcattaatca tttcgcgtaa tgactctaat taaattctat
    10261 ctatcattgc atcgctattc acagctggtt tcaccatcaa tttctcatca attttttatt
    10321 accatgacgt ctccagtcaa atattaaatc gctgatgatt gttaattccg caattacctt
    10381 gcagtcacct ctccttccac ttcatcgcca aatctgcccc tattcactag aaaaaataaa
    10441 tagtatactt agtaaattaa ttacacatct taaaggagtt aaaattttat ggttaatgca
    10501 ttaacaccaa aacatttagc tgaaaagcgt gcaggattcc atgagttgtt ctttgattta
    10561 attttcgtct atgccattca aaaaattgca catgtgattt taacaacaca aaacggttcc
    10621 atttcagctg atttattttt caaatacatc gtcatgagtt tgttcttatg gttaatgtgg
    10681 tctcatcaaa cattttttac aaatcgattt ggtcaagtta cattcaaaga cgtctcgttt
    10741 atgatgttta atatgttcat catggtcttc ctatctaaca gcctatatcc ggactttgaa
    10801 aagacattct tcccattctt cttatgcgtg gcaattatgt atttaagtat cggattacag
    10861 tatttattac atatacgtac agggttagat tatggcgata agcgtacatg tcaagctttt
    10921 gcgagcgtag cctttgtgat ttcattttta tcatttctat cattagtact accacaaagt
    10981 atccattaca tccctggatt tttaggcgta ttcattgcag cgacaggact tattccattt
    11041 cagaaatatc ttgtattatc acctgttaat atgatgcatt tagtagaacg tttttcgtta
    11101 ctaacaatta ttatctttgg cgaggtactg gttggtttag cttcgtcatc atttagcatt
    11161 gatcatttta gttacattta tatattccaa tttatgattt taattagtct tttcggtgta
    11221 tattggatca ttactgaaaa ttatattaat cataaattaa catctatcgg tttccgttta
    11281 tcgtatacgc atctattaat caatatcgct ttaggtgtga ttaatgctgc aattgttttt
    11341 agtaataaca ataaactaaa tgacttgttt gaaattaata tgatgtatat cagtgtttta
    11401 atattctata ttggattatg gttaatcacg ccttatttcc ataatgaact gacaaacgcg
    11461 aagtatattt catattcact aggtatactc gttatgtctt atattgtcag ccttttattt
    11521 aaaggtcacg accatgtaat gattatctca gtatcagttg caaccttctg catcatgctg
    11581 atttatttta aaaatcaacg tctacgtaat aaacatgaat aaatagtatt tacctcggtc
    11641 aaagtattgg aactgaatcc aaaagtgtcg aacttcacat tatacttttg tgacaaactt
    11701 cagttatgct acatgatcga ggtttttaaa tatttccgag gaaatcgtta gcctatacaa
    11761 tcatcaaatg atacttataa ataacatata tcctctaatc cccactatct aaaaggaaat
    11821 aattcccaca caacataacc acctcaaaac aaataaatca atatagatat gtcacctata
    11881 cttatccccc acatttcatt ccacaccaaa aagaacagat gacaatcgta aagatttatc
    11941 acctgctcta aatattattt caaatactat catcaccaaa atcgacaatt atagttcttg
    12001 tttgttaaaa attttaatag acaatatata agaaacgctc gttataacta taccaatgac
    12061 cgcatatgct atgacaacta gccatccttc agtgctaatt ttttcaaatt ctaatgcaga
    12121 tgcaccacta accataccat atataaagaa aaagaccacg aaagaaataa gtagtagtat
    12181 agatgcacat atcataatga cattggagtt ttcagcgcca aatttaaatg tcaatgggaa
    12241 aatgatagag tatgccccta tgataccaaa actcattaaa cctgacataa taccaatcat
    12301 cacactttgg gtcacaatcg tagtcacaac taatccaatc attaaacttg caccgaataa
    12361 gattaaataa aaggcaaagt atgacttaat ataatcacta cgtttaaccg gtaaagtaga
    12421 tacataatac atccatcttg agtctttttc atgtttaata ttatcagtaa taggtgtgat
    12481 taacataacc ccagccattg ccgaactcat caacggatta aatactgcaa agtatcctgc
    12541 agctataata gcgactataa aataaatata tgtttgcttt ctcgttgcat aaaaactact
    12601 taggaacata cctttcatta tacttcacct cgcattatga tttttgtagc gtcatcaatg
    12661 tgttttaaag gctctgcatt ttcaatttct gcataatctt taactaaaat ttgatatttc
    12721 ccctttgaca atctagaagc aatgattaaa tgcttaggaa tttcaacatc tttatcttct
    12781 gtcgtaacaa ttccatattg tgctaaaagt atatctttct gttcagttaa aatcattcgt
    12841 ccatctttca taaacactaa tttatcagct aaatgttcta tatcttcaga aatatgcgat
    12901 gatattaaga tgcctccacc ttgtgcgaca aaatcttcta gtatttccat tacttcttct
    12961 cttccagaaa catccatacc tgcagttgct tcatctaaga ttaataactt cacatcatga
    13021 gaaagcgcaa tcgttaaagc tatcttcatt cgcatccctc ttgaaaaagt tttaatttta
    13081 gtttgtagtg gtaactcgaa atatttgatt aaatcaaaga atttttgact attccaagtc
    13141 atataaatag attgaaatac tttatcaata tctttaatag tcaatttatt aggcactctc
    13201 aaatcatcaa acactacacc tatgtgttct ttatattcga catcatccac ggtatgttct
    13261 tctccaaaaa atgtgatact accgttatct ttatgtcgat tgcctactag aatattaatt
    13321 aatgtcgatt tacctgagcc atttttccca attaatccaa ctgtctcatt agtagaaatc
    13381 gttaaagatg catcttgcaa tttaaaatcc gaatctttgt attgtttgtt cacactatct
    13441 acgtttaaca atgttgtcat atccatgctc ctctttgttt aattttaata aaaacgtctt
    13501 ctcttcaata agtaactaaa ggcgttatat atgaaaatag caatgataag tagcaaacta
    13561 aagctttgat taattccagt agtaattgaa taaagcccaa ttactacaat ggctagtatc
    13621 aatattgata agttgatcgc atatgtttta aataatgcaa ttaattctat atggcgttca
    13681 ccttcatcca atatttccaa tcgcttttca gtgtaattct tatctgcaat ttttggtatt
    13741 ctgtcatcaa accttctatt aaacagtgta aattgtgtat tgaaaatagc actagcaaaa
    13801 aatggtatta aaaagaatag tattgcattt gctgcagcgt gccctaccac aaaaataagc
    13861 aatactaaaa aagcaataac tgtctgcaga atacttaata tacttccatt taaaacataa
    13921 cgattcgcaa gcaactcata ttgatctgtt gcatcgatat ctacctcttt atctacaagt
    13981 tgcttatact tcaatgcacg tctttgattc atcaaaacaa tggcttctac caatatgata
    14041 ataatcgtcg caacaatcga tattacaacg acattgttat atgtcgcaaa cgtcaagttc
    14101 tcaataccaa atccatcaaa attacctaaa attccaccta tgataccccc tacaagtcca
    14161 cccagtaaaa gatatccgat atatcttagt attttcatgc ctcatcctcc tcaataataa
    14221 aaacagtttc caccgtttca ttgaaaatgc gagcaatttt tattgccgtt aatactgatg
    14281 gcataaaatt gtttcgctca attagcgata tggtttgtct tgaaacgccc gcttgtttag
    14341 caagttgcgt ttggtttaag ccatctcgtg ctcgtaattc tttcaatcga ttacgcacat
    14401 cgcatcaact ccttaattac actgtaaatg atatgcgtct ttttgacaac tatatttgtc
    14461 aaatctacac caaaaaatat gattatccac ctatgtatga cattttgaaa caaacacctc
    14521 aacgcctaca agtcataatt gtttactttc gttacacctt cctgcataat taacagcatt
    14581 ctaattttag tatgatgcac gcattttcac taaatcaaac cattcaaagg agactattat
    14641 ggcatttaca ttatctgcaa ttcaacaagc acatcaacaa tttactggtg ttgactttcc
    14701 aaaactattc aaagctttta aagatatggg gatgacttac aatatcgtca acattcaaga
    14761 tggcactgca acatacgtac atcaatcaga agatgatatc gttacgtcat ctgtaaaaag
    14821 taatcatcct gttgctcaaa aatcaaacaa aacaatagtt caagacgtct taactagaca
    14881 tcaacaaggg caaacagatt ttgaaacatt ttgtgatgaa atggctgaag ctggcattta
    14941 taaatggcat atcgatattc aagcgggcac ttgtacttat atcgacttgc aagaccaagc
    15001 tgttatttca gaattaatcc ctcaataaac tatatttata gcaacatttt aattatttca
    15061 taaaatttta ttgataatca ttatcgttcg gtataaagta aatactatat actacttatg
    15121 agtgaggttg attatcatga taactaacac ttttatttta ggcatcacag gcccaacaag
    15181 tcttgtcgtc attagcatta tcgctttaat tatttttggt ccgaaaaaat taccacaatt
    15241 tggccgtgcc atcggttcta ctttaaaaga atttaaatct gcaacagaag atttagataa
    15301 agagtctcac gatacaccca gtaaggaatc gaaacaacag cgagagcaat agcactgacc
    15361 acaccttact ggttcacttt agcgaactac gccatcggtt agtaaaaatt ttattgtcgt
    15421 tcgtcattac ggtcatcgtc gtatatgttt catcattttg gtggatgaca ccattcataa
    15481 cgtatattac ccgtgcacat gtgtccttac atgcattttc attcacagaa atgatacaaa
    15541 tatacgtgat gattatattt ttcatcgcgt tttgtttcat ttcaccagtc atgttttatc
    15601 aattgtgggc attcattgca cctggattac ataataatga acgtcaattt atttataaat
    15661 atagtttttt cagcgtgtta ttattctgtg ctggcgtcgc ttttgcattt tatgttggct
    15721 ttccaatgat cattcaattc gcgttaaaat tatcattaac tttaaacatt tcgcctgtca
    15781 ttggctttaa agcatatttg attgaactca tacgttggtt attcacattt ggtcttctat
    15841 ttcaattacc aatattattt atgggtcttg caaaattcgg tcttatagat accacatcat
    15901 tgaaacatta tcgtaaatat atttatttcg cttgctttgt attagcaagt atcattgcac
    15961 cacccgattt aacattaaat atattactca cattgccact catattgctc tttgagttca
    16021 gtatgttcat cgttaagttc acatgccgag gcaaaccgcc aacacattaa ccttatacga
    16081 atcaatgctg tgattatcgt tatactgcaa taccgaatat ttccaatata gcaacaaaaa
    16141 aacaggcact cggcctgttt tttatttttt aaatttaaag attaagatag caactaccat
    16201 aatataagca ccttgtgcta tcaatggttc ataacttgga taaaagccca accagttaat
    16261 cgttgggaat ccttcaataa catgtcttgg catcgcacct aataattgta acttttgaat
    16321 acttacgcca agcattttga atcccataat aaaaataaag atcgacaaca ctctaaatat
    16381 atagaaaata ggtattaatt taactataaa tctaaataat aatgcaaaga tgattaaaat
    16441 aacgatagct aaagcaatac caataataaa atctttggtc gctagctcac ctatcatccc
    16501 catatagaaa atgataacct cgacaccttc acgcaacaca gatattaaac caatcgtcgc
    16561 taacaatacc aaattaccat tactaatcgc attagcatac atatttttaa tcatgtcatt
    16621 ccaacgtttt gcatttgaac gtttgtgcat ccaaacacca acgataaaca ttaatatgac
    16681 cgcaacgata cctaatcccg cttccatact ttcacgaaga atgccactat tccctaaagt
    16741 ttctacaaac gtaattgcta agataatact cagtacaagt ccggcaattg caccaccaat
    16801 cacacttgca gtccctttct tatcttttac attacgcgtc atggtagtca atgtcattac
    16861 aattaacaac acttctagcc cttcacgtaa aaagataatc atcacatcga cgaagctata
    16921 actatggcca acaacctctt taatttggtt atttaaatct actaaaccat ctttcacatg
    16981 tgctttatta tgttcgtcta atacactttg ataatatggt attttatctt caattttcgt
    17041 atacaaagca ccgtctttag tttgaatttg accttcaaca tacggccaag tttctataaa
    17101 atgtgtaagc gcagcatcag catccgacaa ttgattgtcg tcgatagctt taatcgcctt
    17161 ctctaacgca tcatttaatt gtgatacatg gtattgatca tttgcagacg tattactttt
    17221 tttatcgaca tgatcaatat ttgatttaaa agttgtccaa gcatgtgaca cttttgccgt
    17281 atctaatggt gacttatgaa ttgcaattct aagttgtaat aatgcgactt caatttgtcc
    17341 atattgattt gcgtcataat tgcgaatcac tgtttcatta cttgtccaaa tctgattcaa
    17401 actattgttc aaagattcta attccgcttt atttttatct ttaatcgctt ttgtcatcgc
    17461 agcatcttta gcatcgactt gctgttgcaa tagtttaatt ttagaacccg catctttact
    17521 agccaatttc tcttcataag caattaatga cttcgttaat tgcgaaagtg tatctttttg
    17581 attatcattc gcttttgcat cttcaagctt tctcacatct gatttgacag cattactttc
    17641 actattatct tcaagcgata atttcttaac tgcacttacc acttgctcaa ttgctttctg
    17701 cttattgtca ttcgatatcg aattattaga aagtgcagat ttcgcatccg ttatcacact
    17761 atatacatca ctaatacttt gttgttctgc tgcctgactt ttcagtaacc caaagctaca
    17821 caccatagca gcagttatta gcattgctac aaatttagtc aaataatgtt tcaccaaggt
    17881 atcctccctt actaacacct ggtaatacta aaaatgaagc agaacctcta tgtgtaatat
    17941 attcatttaa tttatcatta ctacctaaat tattttgtat atcgataaat tgttttgtcg
    18001 ctttttgaaa agcaataaaa agtaagcctg tttcgaagtt acctgtgcgg tcatccgtac
    18061 catccacata attaaaggct ctacgtaaaa ttgacgtatt tgcttctttc gctagcctcg
    18121 tatgggcatc tttatcaata atatactcgc catgactatc tttcgctttt aagtcaattt
    18181 catcaaactc tttcccacct gttaacggtg caccactatg tcgtttccga ccaaatgtag
    18241 cctcttgttc ttccagcgca gtacgatccc acgtttcaat gtgtatttga atacgtctga
    18301 caacgcaata agttccatgt ttcgcccatc cgtcatcaat aaacacataa tctttaagtt
    18361 gattattctt cctaggatta attgttccat ctttaaatgc cattaaattt ctaggtgttt
    18421 ccttaccttt agcagagata aaaccagatt gcgcccaacg taccttaaca atatctcgaa
    18481 atggacgaac taaattatga accgcatgaa aggatacttg cgaatcattt gagcatgctt
    18541 gaatcataat atcaccatcg ctgtaatcgt ctattaactg atcattcgga aaatgcggta
    18601 aatctttaaa ggcatcggga attttactag ataacccaat tttcttcatc aaagacttac
    18661 taatcccaaa ggtaatcgtt aacttgcttg cacctaatcc tatcgattca ccggtatcta
    18721 ctggtggcat taaaggattg ttactcggct tgccaactgt atcaccatcc atcatacgat
    18781 cagccataac cgtccacttt ttaaacattg ccttaattgc atctctatct tttgacttca
    18841 aatctaacgc aacgaaattg catgtttttt gcgtgggtgt ggtaatgcct ggttgcactt
    18901 taccataaaa ttcatacgca tctttttccg gatcttctgg tgtattgaac attgatttga
    18961 aagaccacat gctaccaaca ccacttgcgc caattgcaac accggcaccg ccaataccta
    19021 acattttcaa aaatgtacgt cttgaaaatt gcgtactatc gttaacttgt tcataatttg
    19081 tcattcagta atcacagcca ttttacttag tggctctcct aatgcattaa cagcatccgc
    19141 aaatgcttta cggtctttct tcgatacttt ttcaaatgac tcataaccgc cgttgttatc
    19201 tttatattta tccaataatt gattcacttt atcgaagttc atttggatat catcacttaa
    19261 ttttttatct tttttctcta aaataggttt aaataagtca taaatttttt gtgcgccttc
    19321 aacgttcgct ttaaaatcat ataaatctgt atgtgaataa atttcttctt cacctgtgat
    19381 tttagaagtt gcaacttcat ttaataggtc aacagaacct tgtaacatta attttggtat
    19441 gatatctaat gtatcagctt tggcatgcaa ttcttttgca tctttcaata attgttgtgc
    19501 atctttttta gtcacatcat caattttctt atcttcgtat aatgcttttt caatcttatg
    19561 atatcctgac cattcttttt cctttttctc ttctttcata tctgcaagac gtgcatcaat
    19621 tttaggatct aaatctccaa atgcttctgc aactggttca gagcgttcat aatacatacg
    19681 aacttttgga tataacgctt ttgcttgggc catatcatta ttttcaatcg ctttaacaaa
    19741 tttttctgta ccttttaaaa attcatttaa ctgttcatca gtatatttat catatgcttt
    19801 tgttgcttgt tttaattctg ctttaacacc atcatctttt ttcgatgtct ttgaatcatc
    19861 cttcttacta tcgtcgttcc cacatgcagc aattaataac gttgatgcta ataatagcgt
    19921 tgttaacttt ttcattttga cacctcatta tagaaattct attatcccca tagatgtgtt
    19981 cttataaata ttattttcgc attccctaca cgctaaaata atacattaca cttaaaacgg
    20041 ctgttttaaa gcatcctccc ataaacatca tctagttgat aataattatc aaaatcatta
    20101 tattgttata aaataatact gtcaacaacc ttttattttt aaaaattaaa taaaagagcg
    20161 ctgccatttt aaatgtcatc actcttttgt aagtctaatt tgtcattttt ggtcgtattc
    20221 tgattttaat aaactataga tgtaactcga tgaataaata ccatttagta attcattgtc
    20281 acgtaacata ccttctctag taaatcccag cctttcagga atagcccggc ttttttcatt
    20341 attaactgcg gcactaatct caattcggtt taagtcaagc tcttcgaaac aatactttat
    20401 caatgcctca actgcttgtg tcataatccc atgaccctca aattctttat ctaaatagta
    20461 ccctaatgaa gtttttctgt gcatgtgatt aatttcatgt aaaccgatga caccaactag
    20521 cgttccttca taccaaatgc cacactgaaa tccattacca tcagcaaatt gcaaaagtcc
    20581 tcttttaata aatgcacgcg tatctgatgg ttgctcagtt gcatctaccc aaggtaacca
    20641 ttccctaagt gaatttcttg aacgattgac taaattgaaa agcgcttctg tgtcatgagc
    20701 ttctaaaatt tttaatgtta tttgttcatt cactttcatt ccaaacatac catcacatcc
    20761 tcattcattt ttcatataat tctgtaaatt atcattataa taacatatta atgttacgca
    20821 ttgtatctta aatttgttgt ccttc
//
LOCUS       DANRDI010000034        20527 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.034, whole genome shotgun sequence.
ACCESSION   DANRDI010000034 DANRDI010000000
VERSION     DANRDI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.034"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            180..1052
                     /locus_tag="PZ377_000975"
     CDS             180..1052
                     /locus_tag="PZ377_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDM6357860.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1439..2818)
                     /locus_tag="PZ377_000976"
     CDS             complement(1439..2818)
                     /locus_tag="PZ377_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6357861.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3098..3454
                     /locus_tag="PZ377_000977"
     CDS             3098..3454
                     /locus_tag="PZ377_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357862.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3451..3636
                     /locus_tag="PZ377_000978"
     CDS             3451..3636
                     /locus_tag="PZ377_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357863.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3732..3923)
                     /locus_tag="PZ377_000979"
     CDS             complement(3732..3923)
                     /locus_tag="PZ377_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357864.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(3934..4569)
                     /locus_tag="PZ377_000980"
     CDS             complement(3934..4569)
                     /locus_tag="PZ377_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDM6357865.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4644..5561)
                     /locus_tag="PZ377_000981"
     CDS             complement(4644..5561)
                     /locus_tag="PZ377_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDM6357866.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            5829..6359
                     /locus_tag="PZ377_000982"
     CDS             5829..6359
                     /locus_tag="PZ377_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357867.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6425..8029)
                     /locus_tag="PZ377_000983"
     CDS             complement(6425..8029)
                     /locus_tag="PZ377_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDM6357868.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8359..9123
                     /locus_tag="PZ377_000984"
     CDS             8359..9123
                     /locus_tag="PZ377_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDM6357869.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9181..9687)
                     /locus_tag="PZ377_000985"
     CDS             complement(9181..9687)
                     /locus_tag="PZ377_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDM6357870.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(9854..11167)
                     /locus_tag="PZ377_000986"
     CDS             complement(9854..11167)
                     /locus_tag="PZ377_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDM6357871.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(11244..11339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11392..11961)
                     /locus_tag="PZ377_000987"
     CDS             complement(11392..11961)
                     /locus_tag="PZ377_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357872.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(12448..13329)
                     /locus_tag="PZ377_000988"
     CDS             complement(12448..13329)
                     /locus_tag="PZ377_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDM6357873.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(13440..14561)
                     /locus_tag="PZ377_000989"
     CDS             complement(13440..14561)
                     /locus_tag="PZ377_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDM6357874.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(14838..16076)
                     /locus_tag="PZ377_000990"
     CDS             complement(14838..16076)
                     /locus_tag="PZ377_000990"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDM6357875.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            complement(16076..17737)
                     /gene="hutU"
                     /locus_tag="PZ377_000991"
     CDS             complement(16076..17737)
                     /gene="hutU"
                     /locus_tag="PZ377_000991"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDM6357876.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            17897..18781
                     /locus_tag="PZ377_000992"
     CDS             17897..18781
                     /locus_tag="PZ377_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDM6357877.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            18903..19322
                     /locus_tag="PZ377_000993"
     CDS             18903..19322
                     /locus_tag="PZ377_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDM6357878.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(19578..20513)
                     /locus_tag="PZ377_000994"
     CDS             complement(19578..20513)
                     /locus_tag="PZ377_000994"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDM6357879.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
BASE COUNT         6862 a         4035 c         2864 g         6766 t
ORIGIN      
        1 tgctattttt tatctataca tcttaaaaaa atttacagtt ttacaattca atatcattaa
       61 acaaacaaca tattcagctt atttttcatt ctcttacgtt acaagttgta aataccatcg
      121 cgttaaacaa caatagccaa cgctaataaa atatattgcg ttaaagtgga gtgattaata
      181 tgtcaaacgt actaacagaa atagatagtc aatatccata tatgactaaa aatgaaaaaa
      241 agatagctaa atttatacta aattcacctc aaaaggtgat taaaatgagg tctcaagact
      301 tagcaagctt attagatatt agtacatcat cagtcattcg attcagcaag aaaattactg
      361 atggcggttt tcacgacttg aaaataaata tatctaaata tgttcctaaa gcatcatcaa
      421 tttacaatgt tgaattaatg aataacgaaa gcacagaatc tttaagaact aaactccata
      481 cacgtactac acgcgcactt aatcatgcaa ataacgaatt aaatgataaa actattgatc
      541 aaatatgtca ttgtttaaaa cgttctgaga cgatttttat atatggtttt ggcgcatctt
      601 ttgtagttgc taccgactta taccaaaagt tatcaagaat aggtcttaat attcaacttg
      661 ttcaagaaac acatattttt gctacattat tagcaactca caattcgaac gacagtgtta
      721 ttcttattac taacaatggt acgcaaagtg aaatgcaatc aatggttaaa gttattgatg
      781 actaccatat acctataatt acaattacta gtacaaggga taatcccgta gcacaggcat
      841 caaatattgt tttaacttat gggaaaactg atgaaaatga gatgcatatg ggagcaacaa
      901 cttcattatt tgctcaaatg ttcactattg atattttata ttatcgttat gttgcattaa
      961 attatcacgc atcattagat ttcataacac aatctaaaat ggctttagat aactatcgta
     1021 aacatttatc aaatattaac tttaaacatt aacagtctta tataaattct ttaatcatgt
     1081 catataataa tttatgcatc attttcaaag cttacatgtc ttttatcttg tataacatac
     1141 gcatatgacc aatgatttga taaggccatt caatttctta tattaaaaac aaacgcagtt
     1201 gattaacgga ttcattccat atatcaattg cgtttgttat tttaataatg ctatttttta
     1261 ttttaaagcc cacagtaaaa actaacgata atattgccac ttataagttt ttcacactac
     1321 aacttcgatt taagctatat ttataaaaaa atgcgcctac tcataacatt ctttggctat
     1381 ttaactttaa taagctaggg ccaagtttgt cataagataa gcgcacatta taatcatact
     1441 attttgcttt tacattattt ttattatatc taataaaata ataaatcgtt acaatcgtta
     1501 agaagatgac accaataagc actgatattc tagtttcaac attaaatacc ataccaacca
     1561 atactaatat taagaataag attgttaaat agttagttac tgcgccacca ggcattttga
     1621 atgggtgacc ttccaattct tcagggtgta attttctaaa acgtaaatga ctgaataaaa
     1681 tcataaacca aggaatcata cctggtaaaa tagacgcact ataaacgtat acgaaaatac
     1741 tcttcgctcc atcgatatat aaaggtaaaa ttacgtttaa taaagcacca attaaaatac
     1801 caagagatac tgctacaaca gtgtacaatg gtacaccatt tctcatgatt ttagtaaata
     1861 ttttcggcat ttcaccttta tgagcaagtg tataaatcat acgactcgca ctgaaaatac
     1921 ctgagttaca tcctgacatt gctgcagtta atacaacaaa gttaattaat ccagctgcaa
     1981 atgtaatacc gattttagca aatgttgcaa caaacggact tccaatgtct cctaattgat
     2041 tccaaggata cactgaaaca ataacaaaaa tagcgcctaa atagaaaatt aaaattctcc
     2101 agataacacc attcactgct ttaacgatat ttttctgagg atctttagtt tcacctgccg
     2161 taatacctat cagttccaca ccttggtatg aaccaattac aattgataat gcaaagaaga
     2221 aaccaacaat accatttggc ataaatccgc catttgtcca tagattagaa atacctaccg
     2281 catggccacc atttccaaaa ccaaagaaaa taagaccaaa accagcaata atcattaaaa
     2341 taattgttac aactttaatt aaagcaaacc aaaattcaaa ttctccaaac gcttttaccg
     2401 agaataaatt cgctgccatt aataataaaa tagcaataac accaggaatc caagttggca
     2461 attccgggaa ccaaaacttc atatattctc ctactgcgat gacctcactc ataccaacta
     2521 caatccattg gaatatatta ctccatgctg tcatataacc tgctgcagga tgtatataat
     2581 cacttgcaaa tgttgcaaat gatcctgttg tagggtttaa ataaatcatt tcccccattg
     2641 ctctcatgat taaaaataaa aagatacccg caattaaata tgcaaggata actgatgggc
     2701 ctgtccattt aattgtactt gtcgcaccca tgaaaagccc cacgccaatc gtaccaccaa
     2761 gtgcaatcat ttgaatttga cgggcactca atcccctttg tagttcattg ttctccataa
     2821 cttcttaccc catctctgat tgatactaaa taaatccata aaaaaactta ttgccgtaat
     2881 tatactataa attatcgatt ttggtacatt aatatttgca taccatgcaa tcgtttacag
     2941 aactactata gcaggctttt gcaactttgc aaacaattat tttgtgattt ttctaaaaaa
     3001 ttaaaaaagt tgctcaagta ccgttgagtc acatacaact tcagctattc ttggtaatta
     3061 tgatataatt ttaataatat taaaaaggag tatatcaatg ttaaaacaaa tactgctacg
     3121 tgctacaaaa atatcggtac tctttgctgt agcatttttc atcataaatt atatcggtat
     3181 ggaaaaacct gacatcctat atttagttgg aagaactatt attgcaacac ttgctttcat
     3241 actcatttgt cttacagtat ttactattat taattctcca gagcgcaaga ttaaactcgg
     3301 tacgacttta ccgattgcat taattattgg tatcattttc ggcgccatat ttttaacggt
     3361 acaaatcggt gtcatcactg ggttaatcat cggtgtgatt gctacgttta tttgggaatt
     3421 gattgaaaaa aataaaggag gtcgttcatc atgacaacag cattaattgg tgtaattgta
     3481 ttaatcttac taattattag ctttgtgcca aactaccgtg ctatgaaatc agcaaaagaa
     3541 caaggaagta atgcaacaag agctacaatt atggtaggca tagatattat cttaattgta
     3601 cttatcatcg tgacgttatt attaaaatat atgtaactat ttaaaccacc ctatctcata
     3661 cataaacatg aacgttctac catttcacat acatatggtc gtccatttaa tgaggtaagg
     3721 tggttttctc gttaaagttt accttgtaat agtaaaaaat agccaattgt tgcgagtatt
     3781 aagtaaatca ttgaaccaac aacaaaagat cttacttgca cgcgaaatgg tgtctgtttg
     3841 ttaaatatag gaccaatcac caaatttcca acgcctaaaa ttactggacc agctagaaaa
     3901 aacaatataa tgtaacccat catgatacgc ctcctatttc gtaaagaaac gcgctacaat
     3961 ttgatcgtaa tcaatatctt ttgctacata atctacatta gaaaagtcct gtgcgcttgt
     4021 cattttattc gtattaacaa taacatctaa tccagctgca atcgcggctg ttgcaccatt
     4081 tacagaatct tcaatagcca aacattgtgt cggcatataa tttaattgtt gtactgcaga
     4141 taaatataat tcagggtcag gtttaacttg ttcaacatct tctctaccaa caactacctc
     4201 tatataatcg tctagaccta atgctttaaa ggtgggcatt atttccgctt tcacactact
     4261 tgtggcaatt gccataggaa tgtgacgttg ctttaaaaat gccatcaatg atttaatcgt
     4321 atcaatcatc ggtaatgttg tactagtaag atgatgttct tcataaagtt tatctttatt
     4381 ttccgaacca atcgctttaa ttaaatggtc atgcaaatct gtagctgctc ctccaataga
     4441 agaacgataa aaatcaatgc ttataggatc ggcattatgc atctctaaat gtttattaat
     4501 aacattaaat aaatgttgtt ccgtatctat tattgttcca tcgaaatcaa atataactgc
     4561 tctatacata tatttactcc tctatttttt ctctttatca aagcttaaca tagtccgatg
     4621 accacttcat tctattagtg caattataac ttttttgacc aatactttgc taaaataggc
     4681 cctgttatat tatgaatgaa actaaacact gcgcctggta ctgctgcaat tggattaaaa
     4741 tgcaatgctg ctaatgacac agctaaacca gagttctgca ttccaacttc aatactgact
     4801 gctttttgat cttgtcgatc taattttaaa agacgagcta accaatatcc aatcgtatac
     4861 cctaatacgt tatgtaaaat aactactaaa aatattaata atcctgtagt taagatttgg
     4921 tgcttactgc cacctacaac tattgctaaa attaatgaaa tagcaacaac tgatataatt
     4981 ggcaaagctg tagaagcttt ttcagcaatt ttacgattaa taatttgcaa aacaatacct
     5041 aaagcaattg gaattaatac aacttgaaca actgaccaca acatactcac gaaagatact
     5101 tccaaccatt catttgcaaa tagatatatt aacgcaggtg taacgaatgg cgctagcaac
     5161 gtagagaccg tcgtaataga aacagaaagt gctacgttag ctttggctaa ataactcatt
     5221 acattacttg atgtcccacc cggacaacat ccgactaata ttacgccaac agcaatatca
     5281 ggtggtaaat gaaaagactt tgcaattata aatgctaatg tgggcataat actgaattgt
     5341 agacagacac caataattac tgctctaggt gctttaaaga ccattttgaa atcattaggt
     5401 gtaattgtta atcccatacc taacataaca ataccaagta aataaggtac ccatttacct
     5461 aatgtcgcaa catgttgtgg gaaaataaat ccaataacag ctgctactaa catccatacc
     5521 aaaaaactct tcgtggcaaa tatactcaat ttttgaaaca ttgttaacac atcctcgtcg
     5581 ataatattga aaaatattac cataagacca gtaaggttac aattactttt ctgaaaatta
     5641 agattattta gtcattctct tcttattgca aatattttat cactgataaa caattatttt
     5701 aaacttcatt caaaagtctt agtgaataaa gaaaaaaagc gtggtaaaat caacttgaac
     5761 aaaattaagt cacctttata aatattaaac gtgtatttaa tgccatgtat tcctcgggag
     5821 gtcctcacat gttaattgat aaattcgaaa catacatcat caacatcgcc ggtttaaatg
     5881 accgtactac tagaaaaaaa ttaagtaagt tatgcaaaag tgtacaattt tgtgatgcgt
     5941 tgcaattctc aataaacaaa cagtttaatc agtatgtatt agaaattagc ttaccaaagc
     6001 aacaacttcc atacttcatt tcatttttaa gttttcatca gtatagtatt ttccaagtac
     6061 tttcaccaaa gaaaataaat gaattacttg attctgataa tttatatcaa tctgcaaagc
     6121 gtttcgatat caatattgat ggcttgcaag atgctttcat taaagacaaa gtcatcgata
     6181 ttatgaatat gttccaaaat cacactgata tcacttatac gttgaataag tctcatgcac
     6241 atattatatg tacgccagaa atatttgcta aattgttaca tacgattgca actagaaata
     6301 tcgacattct atctgccaat tatagatcgt catctatgag caaagctcgt atatcataat
     6361 aaaagacaca tctatacgat gatcatttac aatgattgcg tatagtgatg tgtctttttt
     6421 tgtattattt atcttcggac gattgatcct caaccatttg ttcaaatgct tcacgaactt
     6481 gtggtactgt catacctact acaacttgga tactcttacc actctttact aatccgtgtg
     6541 ccatttgttg atgcgtgaaa tattccgtat ctgcaacttt actttcatca taaacagtta
     6601 agcgtaatct tgttgtacag ttagtaacat ctttgatatt ttctttgccg cctaaaccgt
     6661 cgaggtaata tgcagctttg tcttcgtatt cattaccaga tgaagcacgt ttagaatcta
     6721 cgctatcacc ttttttattt ttgtaatctt gcttagaaaa taatttaact tcctcttctg
     6781 ttttcttacg tccaggtaat ggaatatcaa atttcaaaat taagaatctg aataagaaga
     6841 agtaaatcgc tacaaatatc agaccgataa taacttgtgc aacataagta ccccaatgtt
     6901 ctttacctaa tggcaaccag tttgttgaaa taaagtctaa caagccacca cccatgttac
     6961 ctacaacacc aaatgcgtac attaaagtat ccattgatgc tgctaaaact gcgtgtaaaa
     7021 caaataagta tggtgcgatg aataagaatg taaactctaa tggctcagta atacctacaa
     7081 cgactgctgt taatgtggca ggaataagta gtgccgccac ctttttacga ttttctttag
     7141 gtgtcgtagc atacattgct aaagcaatac ctaaacatcc aaatactttc ccatttcctt
     7201 gaagcataaa gccataaggg aattgctctt ttaatggctt agtactctta gcaaactcat
     7261 ttaaatgttg taaccattca gctttcaagc cgtgattgac tactactggt ccaacttcaa
     7321 ttggtgcata aataaagtga tgtaatcctg ttggtattaa tacacgttct aagaagtgat
     7381 acaaccatac accgatatag cctgaagcaa tgataaagtg ttgcattgaa ccaataccgt
     7441 gctgaacagt tggccaaata acacaagtaa ttgctgctaa tggtaacatc acaaaaaatg
     7501 aaatcgtaac tacaaatgtt aaaccttgga atacaccaac catttctggt aatcgtttac
     7561 tatagtatct attgtgaatc caagtaataa tagctgaaat aataatacca cctagaatat
     7621 tcgtatccaa tgtggcaata cctgcaattg attttaaacc aggtacattt tcaacgcctt
     7681 tttctaaatt agcgccaaac gtatgtggcc attgtgttaa aatggcattt ataaatgtat
     7741 taaacattaa gtaacccatc aatgctgcaa gtgctgcatg acctggtgct tttttagcta
     7801 aagaaagtgg tagacctaca acaaatacaa cttccatatg cgtaaaaata acccagccac
     7861 cagattctat tactgaccaa aatttaaacc aaaacgtatg ttgatctgct aaactcccca
     7921 tgattgtagg atttttaaat aatgtcgcaa agccaagcac tatcccaaag aaagcgaaca
     7981 ttaataccgg tacaatcatt gcactaccga aacgctttat cgcattcatc ttctattccc
     8041 tccatatcat ctttcctaac aatacatcta attagattca tttataaata gatgtcttac
     8101 tatttaaata taatatatag taaacgctta cacacctaca acgacattga cgtattttga
     8161 aagtattttg tataatcaga ttatcttttc atatagtgaa aattttttca cgaccttata
     8221 tatgacatcg ttgtatttgt aatacattcg ttttaaacgc ataatcaaac ctatatcaat
     8281 acacaaatat atataatgac atacaagatt ttaatgtaat aacgatctat tacacattta
     8341 ttttcaagga ggttgaatat gtttttagat gaacacatta atcgaaactt tgataaactt
     8401 aatgataatg atttacatat cgctcacttt atcaatacac atatagatga atgtaaaaat
     8461 atgaaaatac aagatttagc gcaattcaca catgcctcga atgcaaccat tcacagattt
     8521 acacgcaaat taggttttga cggttatagt gattttaaat cgtaccttaa atttgaaagt
     8581 aataagacac accaattacc ttctaactct atcgatagtt tcaaacaaga aatcgatagt
     8641 acattcagtt atttagaacg cgttgattac cagcttctta ctaaaaaaat gcatacagct
     8701 tcaacaatat atttgtatgg tacagggcgt gcacagttaa acgttgcttc agaggcacag
     8761 cgtatattgt taacgttaca taatcatgtc attgtacttc atgatgaaca tgaacttaaa
     8821 atgattttga acaagagtga acctaatgat ttattcttta ttatttcatt atcaggtgaa
     8881 acacatgaat tattagaagt aacaaacttg cttcaactta gacaaaaata ctttatttct
     8941 gttactacaa tgaaagataa tacccttgct caaaaagcaa attacaacgt ctatgtatcc
     9001 agtaacacat tctacttaac tgatggtaca gactattcta gcttcatcag ttatcatata
     9061 ttctttgaaa cacttgtaag aaaatataat gagtatttac tacataatga tatttcaaat
     9121 tagaaaaaag gaagttatga tttaatggcc ttgagcctat cataacttcc ttttatcatt
     9181 ttattgttgt gttgatgttt cgataacgtg gtacatctta tcaaacatca attcgaaacc
     9241 atgcaccatg gcatcatgat attctttttt cttttgcttg tattctaaat tagtaaatcg
     9301 tctttctttt tcaactaatg aacgataata aaatagcatt tgggtgccac ctgtttcacg
     9361 ttcaaaaaat tctacctcaa tgacatcttg cgtttcactt agtccaggca taccgatagt
     9421 catcttaacg tattcatcca taactaaaga ttcataaatg ccttcaatca catttacttt
     9481 gccattacgt tgttgatcta caatacgata tttaccgcct tctttaacgt ccgcttcaat
     9541 ctctttattc gttctggctg atgtcataaa ccattgtttc aacaaatctt tctttgtcca
     9601 agcttcgtat actaactctg gagaaaattt ataaagcttt tcaatttcaa cttcgacatg
     9661 ttcattctct acattaaatt ttgccactgt tgtccaccca ctttcgctct tacttttatt
     9721 ttaacgtatt tttgctcagt tccaaacata gatgatcatc atttttaaaa gattagcgtt
     9781 atacggtgag tacaacatga tctgttaata taacaagcca ccttacttgg ctacatcgat
     9841 atattgttaa gcattaatgt ttcatttctt gactagtgtt cttttttagc tttggaaaat
     9901 taaataaaat cgcaataagt ccgcatacac ctaataatat aggataaatg ctgtatggga
     9961 ataacattaa cggtgaaata ccagctacac cagccgctga aatgacttgc gggctatatg
    10021 gtaataaacc ttggaagcag cctccaaata tatcaagaat acttgctgat ttccttgaat
    10081 ctacatcata ttcatctgca atatttttag ctaaaggacc tgacataata atagagatgg
    10141 tgttgtttgc cgtggcaata tctgcgacac ttaccaaact agcaattcct aattctgcgc
    10201 cacgctttga tttcacttta gagcgaacaa attgcaacaa ccattcaata ccaccattgt
    10261 gttgaataat accgactaaa ccaccaatta gcaacgcaat catagcaata tcttccatgc
    10321 ttataatacc tttggacact gcatctagta gccccatcca accgaatgaa ccatctatga
    10381 gaccaatgat tccggctaat aatgttccgc caatcaatac gataatgaca tttacaccta
    10441 ataatgctaa taccaatact aagatatacg gtacaacttt aattagatta taatcatagt
    10501 ttttagcatg atttaaagaa atgccattcg ttaagaaata cagaataata atcgttaaaa
    10561 tagcacctgg caatacaatt ttaaagttta ctctgaattt atctttcatt ttcgtatgtt
    10621 gtgttctaac cgcagcaatt gttgtatctg aaatcattga tagattatcg ccgaacattg
    10681 cacctccaac aactgtagcc attgctagcg cagttggtac atctgtcgct tgtgcaaatc
    10741 cgtaaccaac aggtgctatt gcagcaacag ttcctacaga cgtccccata gatatagaaa
    10801 caaacataca aatcacaaac aatcctacaa taattaaatt ttctgggatt aatgatagtc
    10861 ctaaattaac tgtcgacttt acgccaccca ttttttcagc tgtatttgaa aatgcacctg
    10921 ctaaaataaa aatcaacatc attaaaacaa tgtttgaatg gcctgcacct ttcgtgaaga
    10981 cctcaacttt tttagcaaat gattcttttc gattcattaa taacgccaca attaccgtta
    11041 tcgtaattgc aacatttaat ggcattgaag taaaatcacc tgtgataata cctacgccta
    11101 aaaacaacgc cacaaataat aacaagggga ataatgccca agcattgctc tttttatgta
    11161 cttccatcct ttttacctgc tttccaatta aaaatacctc tttctcacaa acgatgaaga
    11221 aagaggtttt catgtgcttt acctgcttat cttcaaacca ttacggttac tggaattggc
    11281 acattcgaga tgttgccgag gcttcatagg gccagtccct ccacctctct agataagtga
    11341 tgcttattta cgtttacgtt acaagataat ccttagtacg tcaatcataa attaatcagg
    11401 agtcgtataa tatttttcat aaacaatcat tgctactgta ataataatca aaacaataat
    11461 gctaataaca agtaaaagcc accatttaag cattaatgca ataaaaatga acacgataga
    11521 cacacttact aatattaatg atatgacttt aaattgctga acacgttgct tggagatgac
    11581 tttcaactgt ttgtttgata gacgcgtatt ttttatactg attcccagta tattttctaa
    11641 tatttgaacc aatacgatac ttattgcaaa tataataatt ggtaaaacat catagctccc
    11701 tatagttaat gtataaatta caaatccaat gtaaagtaac cctgagacaa aggataaaaa
    11761 gtatgcgacg tatttgttaa acttaatgat atgcttttta acgttttgat gtgtaaacca
    11821 tacattcgaa acgatcgcaa ctgctacaaa taatgtgaat actatatata atggtaattt
    11881 ttgttcagga aaaacagtcg ctattccaaa agctaatgct aaaatcaaaa ataatatagc
    11941 tctagatact attaatgcca taataacaac ccctttgttt aatatcgagt ttgcaaattt
    12001 acgtttatca gcgtttctat gatcagtact tctacgggta gcgtttctat gtaatttaca
    12061 tcatcttaac atataaatac ttcgctattt aattgaaaac atatcctatt attctttgtc
    12121 cgttctgacg tttaatatct agccttaggc atttcacttg ttaatgaatt taactttctt
    12181 ccactaaccg tccctaaacc caatcccgca acagttttta actttttcgt tgttgtcctg
    12241 acatcctcat taagaaagtt tattctgctt aaaacttata atccacaccc tgagcaaacg
    12301 ctccttatga cagagtatta aaataagccg ataaagatac acacctttac cgactattta
    12361 aaatacactt caccaattca ttttaattta atggattgaa gtaactaaat taatattatg
    12421 ttgttcaatt aaaagcttca tacaaaccta atctatttgc actccaccgc taacaccgaa
    12481 cacttgtccg gttgtataac ttgattcttc tgacgctaat agcacataag ttccacataa
    12541 ctcaacaggt tgacctgcac gacctaaagg tgttttttga ccaaatgttg ggattttact
    12601 ttgaggttgt ccaccagaaa tttgtaatgg tgaccagaat ggaccaggcg ctacacagtt
    12661 cactctaatt ccttttggtc ctaattcttc tgaaaaactt ttagttaatg aaataattgc
    12721 tgcttttgaa gcggcataat catgaagaat aggactagga ttataacctt gtacagatga
    12781 tgtcgttgta attgacgcac ccggttttaa atattccaat gctttttgaa ctgtccaaaa
    12841 tagcggatag acattcgttt caaatgtttc tgtaaatgcc tcagttgtaa atccatgaat
    12901 atcatcatga tactgttgat gtccagcaac taaagtaaca ttatctaagc cacctaattg
    12961 ttgatatgct tgttcaacaa ggtcatagtt gaactgttca tctcttatat caccaggaat
    13021 taacactgcc ttttgaccac tttcttcaat cacttggcgt acttcttgtg catcttgttc
    13081 ttcactcgga agatagttaa tcgctacatc tgcaccttct ttagcatacg caattgctgc
    13141 tgcacgccct attgctgagt caccacctgt gactaatatt ttatagcctt gtaagcgttg
    13201 atgaccttgg taagacgttt cgccacaatc gggtgctggc gtcatttcag attgtaaacc
    13261 cggtacctct tgttcttgtt tttcataatc cgttgtttta aattttgttc taggatcttg
    13321 agctgccatt tttttacatc tccttattcg cttaatggtt attatttacc caatcttcct
    13381 aggaacttaa tcatgattac actaaaaatt actttcttct ttataaaaac aagctcgaat
    13441 tattcatgca atagtctctt tacaaattca acaaaatact caggtacttt ttccagaatc
    13501 ctttcatccg gtttatattg aggatgatgt aaatcatatt cactatgaga accaattaac
    13561 gcaaatacac ttggaaaatg ttgactataa cctgaaaaat cttctccaat cgtaagcggc
    13621 tgttccatca tttccacctt atatccaaca tgttgggcta ctgcaattgc tttatgcgtc
    13681 aatgcctcat cattcatcac agcgccaggt aaatgcgtat aatttaaatt gattttcata
    13741 ttatatgctt gagccaatcc gtccgcaata tcttgtaatc gtgtttctac aagctttcgt
    13801 accacaggat caaaactacg cactgtgcct tgtacatacg catgatcagc aatgacattc
    13861 caagtattac cacatgatat ttgtccaatt gttactaccg cttcatcaaa cgcagataga
    13921 tttctactaa ctatggattg aatactatta atcaattgcg ccaacacaat aactggatcg
    13981 ttgcattgtt ctggctttgc agcatgacca cccacgcctt taatatgaaa ctcaaaacga
    14041 tctactgctg atgtaattgc ccctgttttg attgcaaatg tacctaccga acgcgatggg
    14101 tcattatgaa aacccaatac tgcttgtaca tcttttaatg catgtgtttc aataatttta
    14161 aaagcgccat gtcctagttc ttctgctgat tgaaaaatga atttaacacg cccagtaaga
    14221 gtgccctcaa tttcttttaa ttttacagct gtagccaaaa tactagccat gtgaatatca
    14281 tgaccacacg catgcataac accttcattt tcagatttaa aatcctgttc aacaagctct
    14341 tgaatcggta aagcatcaat gtcagcccga acagctatac aactcagacc ttgtcccact
    14401 tcagcaacaa gcccagtcgc aagtggtaag tctaatattc taatatgatg ttctgttaaa
    14461 atatctttaa ttttttgtgt agtcttaaat tctttatcgg atagttctgg aaattgatga
    14521 aaataccttc tccaggtaac agcttgatct tttaatccca tcggtcattc cccttcctta
    14581 agtcaatgat atgttgtcta ccctacgatg atcatctttg actattaaac gatgatttca
    14641 caacaatgta ctcttgttaa ttgctttcgt taatgataga cagttgttta ataatatcgt
    14701 aacactgttg tcaaactatt ctaactttta taattgagac tctatacaaa aacgtgttct
    14761 cgaatatact tgtttttaca aaccacaaaa agctctaaac attagtttaa accaatgctt
    14821 agagctttct aattatttta tgctttaaaa gatactgtgt tatctacgat gaccttaccg
    14881 tctttaataa ctttttctgc gtgattgata ccaaaatgat atggaatata ttcatgattt
    14941 ggtgcatccc aaattactaa attagcctta tcacctgtgt taattgtacc cgcgttaatg
    15001 tctattgctt tagcagcatt gaccgtaaca gcattccaaa cttcattagg tgatagcttt
    15061 aatttcaagg ctgcaatcgc cataacaagt tgtaagttgt ttgtgacact actaccaggg
    15121 ttataatcag ttgctaatgc aatcgcaccg ttattgtcaa gcatgcctct tgcatctgca
    15181 taatcttctt tacctaaata gaacgtcgtt gcaggtaaga ggacagctac agtatcacta
    15241 tttcgcaact tttcttttcc tttatcacta gaagctacta agtggtctgc tgatattgct
    15301 tgttcatcaa ttgctaattc cagtccgcct aacggatcaa tttcatccgc atgtattttc
    15361 actttaaaac ctgcttcttt ggctttttgc atataatgtt gcgattgttc tattgtaaat
    15421 acacctgttt cacagaaaat atccgcaaag tctgcatatt gttttacttc cggaagtaac
    15481 gcaatcattt cttctaaaaa tgcctcattt gaacttgcct ctttaggtac agcatgaggc
    15541 cctaggaaag tatgtttcat gtctaaatca tatttctcag ctaaacgatt agacactttc
    15601 aattgcttca gttcattttc tctatctaat ccataaccac tcttactttc aactgcaagc
    15661 acgccgtgtt taatcatagt aagcaaatca tgctctgctt ttttaaacaa gtcatcttcg
    15721 gatgtttctc tagtagcatt aacggtagat aatatgccac cacccatttc taatatttca
    15781 aggtaagact taccttgacg ttttaatgac atctcatgtt ctcgagatcc accaaatgtt
    15841 aaatgggtat gtgcatctac taatgctggg gacactacct tcccactagc atcaatcgtc
    15901 tcagtcgcat cgtagtcatc tgtatgtgtt ccagcatata caattttgcc atctttaatg
    15961 acaactgtac catttttcac aacatttaat tcatctaatt ccttaccctt caaaggttta
    16021 tctgttgatc tcggtaaaat taattctgct atatgattaa ttattaaatc attcattact
    16081 tatcacctgc tttatcaatc attggaatat gaataccttt ttctttagct gtttgaatag
    16141 cgatgtcata gccagcatca acatgtcggg caacacccat acctgggtca gtcgtcaata
    16201 cacgttccaa tcttctttca gcacgctctg atccatctgc tacaacaacc atacccgcat
    16261 gaagtgaata tcccatgcca acaccgccac cgtgatggaa tgaaatccat gaaccacctg
    16321 cagctgtgtt aatgagtgca ttcaatacag cccaatcacc aaccgcgtca ctaccatctt
    16381 tcatactttc tgtttcacgg ttaggactag caactgaacc agcatctaaa tggtctcgtc
    16441 caataacaat tggtgctgaa atttcaccgt cacgtacaag acgatttaaa gctaagccca
    16501 ttttcgctct ttctccatag cctaaccaag caatacgtga tggtagtcct tgatatgaaa
    16561 ttttttcttc agctaaatca agccatctta ataacttttc attttctggg aaaagtttgc
    16621 gcatttcttc atccgcacgc tcgatatctt ttggatcacc actcaacgca gcaaagcgga
    16681 atggcccttt accttcacag aataatggtc taatgtaagc tggtacaaag cctgggaagt
    16741 caaaagcatt tttcactccg ttattgaagg ctacttgacg aatattgtta ccataatcaa
    16801 atgctacagc gccacgtttt tggaattcaa gcattaattc aacatgcttt gccattgaag
    16861 cttgtgacag ttcaacatat tttttcggat ctttttcacg caatactttc gcttcttcta
    16921 cagagtatcc ttgtggcaca tatccattta gcggatcatg tgcacttgtt tggtcagtaa
    16981 taatgtcaat tttaaatcct ttttctagaa tcgcttgatg gatgtctaca gcatttccaa
    17041 ctaacccgat tgataatcct tctccacgtt ctttcgcctc ttctgctaat tttaatgctt
    17101 catctaaatc agctgtttta acatcacagt atttcgtatc aattcgctta tcaacacgtg
    17161 tttcatcaac atccacgcaa attgctaccc catgattcat agtaattgct aacggttgcg
    17221 caccacccat accacctaaa cctgctgtca gtgtaacagt gcctgctaaa tctccattaa
    17281 agtgttgatt acctagctcg gcaaatgtct cataagtacc ttgcacaata ccttgagaac
    17341 caatatatat ccaactaccg gctgtcatct gtccatacat gattaaacct tttttatcta
    17401 attcattaaa atgatcccag tttgcccatt caggcactaa tactgaattt gaaattaata
    17461 cacgtggcgc ttcttcatgt gttttaaata cagcaactgg ctttcctgat tgtactaaca
    17521 ttgtctcatc tgattctaat tctcgtaacg ttttctctat tgcttcaaaa gcttcccaat
    17581 tacgtgctgc ttttccaata ccaccataaa caactaaatc ttctggtctt tcagcaactt
    17641 ctgggtctaa attgttgtat aacattctaa gtactgcttc ttgttcccaa cctttacact
    17701 caatactcaa accttttttt gcttgaattt ttctcataaa attcgctcct gttcttttaa
    17761 gaagttaatt ccactaaatt taaaacgctt acattattat cttcaatatt cattatagta
    17821 tgttaaaata tagccaacaa atataaataa actaattatc catagcttga atctataaat
    17881 aaaaggagca aaacacatga aaattattca gttagaatac ttcttggcta tcgtgaaata
    17941 taatagtttt actaaagctg cacaattttt acatattagc cagccatctt taactgctac
    18001 gattaaaaaa atggaagcag atttaggtta tgacttattt acacgttcaa caaaagacat
    18061 caagattacc gaaaaaggaa tacagtttta tcgttatgcg agcgaattag ttcaacaata
    18121 tcgatccacg atggaaaaaa tgtatgattt aagcgttaca tcagaaccaa ggataaaaat
    18181 tgggactctt gaatctacga atcaatggat tgcgaattta attcgaaagc accattccga
    18241 ctaccctgaa cagcaatatc gtttatatga aatacatgat aaacatcaat ctatagagca
    18301 attactgaat tttaatattc atttagctat aacaaatgaa aaaataaccc acgaagatat
    18361 aagatccatt cctttatatg aggaatctta cattttatta gcacccaagg aaacatttaa
    18421 aaatcaaaat tgggtagatg ttgaaaattt gccactcata ttaccaaaca aaaattctca
    18481 agtgcgcaaa cacttagatg actattttaa tagaagaaat attcgtccaa atgtcgttgt
    18541 agaaacagat cgattcgaat cagcagttgg atttgttcat ctcggcttag gttacgctat
    18601 cattccgaga ttttattacc aatcatttca cacgtctaat ttagaatata aaaaaattcg
    18661 tccaaactta ggccgaaaaa tttatatcaa ttaccataaa aaacgcaaac actccgaaca
    18721 agtacataca ttcgtacaac aatgccaaga ttatttatat ggacttttag aggctcttta
    18781 acttaagtta ttagagcctc ttatgcagtt gcgcagatca tcgtataaaa attaatgacg
    18841 tcatttcaaa aatcgataca aaaataattt attataaaaa ttctaagaaa gaagtgaagc
    18901 agatgttaaa atctattaat catatatgct tttcagtcag aaatttaaac gattcaatac
    18961 atttttatag agatatttta cttgggaaat tgctattgac tggtaaaaaa actgcttatt
    19021 ttgagcttgc aggcctatgg attgctttaa atgaagaaaa agatatacca cgtaatgaaa
    19081 ttcacttttc atatacacat atagctttca ctatagatga cagcgaattt aaatattggc
    19141 atcagaggtt aaaagataat aacgtgaata ttttagaagg aagagttaga gatattagag
    19201 atagacaatc aatttacttt accgaccctg atggtcataa gctagaatta catactggca
    19261 cacttgagaa cagattaaat tattataaag aggctaaacc acatatgaca ttttacaaat
    19321 aaggtgtcat tataaaaagg cctcttgaac tccgttaaaa ttttaattaa ttattatata
    19381 ataagagaac ttttcaaaca atacagttgt tatttttgct atttcaacaa acataaataa
    19441 gcagtaagat gactacaact taagagtctt cttactgcaa ttatttttca aatatatcaa
    19501 cgttaatata acttctatta agaaatactc acattctgcc ctgcaatgca aatctcgtca
    19561 catataaata tttttaatta ttttaaaaaa tgatgcacta aattagcaac gagcttagca
    19621 gttctattgt cagcgtcata tgttggattc atctcagcaa tactaactga agacacctta
    19681 tcacttggaa taatacgttt tgctaattca agaacagtat gtggatacaa acctaacact
    19741 gccggcgcac ttaccccagg cgcaaacgca ctatcaatga catccataca aatcgtaaac
    19801 ataatgacat catgttcatg tacaaaacgt tcaatcatat ctttaattgt tggtgatacg
    19861 tgactcaata attcatctgc aaagacataa tcaatctttt tctctttagc ataatcaaat
    19921 aaactttgcg tattaccacc ttgagcaata ccaagcacta aataatctgt gttttcatct
    19981 tcttctaaaa tttgtctaaa gctcgttcca gatgtagatt gttgttcagc acgtgtatca
    20041 aaatgcgcat caatatttat cacaccaata gattgtgttg gatagacttt acgtgttgct
    20101 aaatattgag catacgcaat atcatgtcca ccacctaata aaaatgtttg tctatgatta
    20161 gcaattgact tcgctgcaag catagcaaat tctttttgag tatcaattaa ttcctcatga
    20221 tcatgataaa catttccgta atcgactaaa gtttcacatt gattcaaatc cggcaaacct
    20281 gcaaatgctt gtttaatcgc atctggtcct tcttttgcac caatgcgccc cttgtttaaa
    20341 gcaacacctt tgtcaacagc atagcctaat ataccgaccc ctgatggcat actactcttt
    20401 tccagcttag acaaatcttc aaatgttact gtttgaaaat gtctaaattt tttcgggtct
    20461 gtttcactat ctaaccttcc agtccataaa tttggttcac cttgcttgta cacagcattt
    20521 ccccctc
//
LOCUS       DANRDI010000035        19524 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.035, whole genome shotgun sequence.
ACCESSION   DANRDI010000035 DANRDI010000000
VERSION     DANRDI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.035"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(145..717)
                     /gene="raiA"
                     /locus_tag="PZ377_000995"
     CDS             complement(145..717)
                     /gene="raiA"
                     /locus_tag="PZ377_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDM6357880.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(778..1452)
                     /locus_tag="PZ377_000996"
     CDS             complement(778..1452)
                     /locus_tag="PZ377_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDM6357881.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(1445..2527)
                     /locus_tag="PZ377_000997"
     CDS             complement(1445..2527)
                     /locus_tag="PZ377_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDM6357882.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(2891..3757)
                     /gene="fakB1"
                     /locus_tag="PZ377_000998"
     CDS             complement(2891..3757)
                     /gene="fakB1"
                     /locus_tag="PZ377_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDM6357883.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            3901..4542
                     /locus_tag="PZ377_000999"
     CDS             3901..4542
                     /locus_tag="PZ377_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDM6357884.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(4712..5767)
                     /locus_tag="PZ377_001000"
     CDS             complement(4712..5767)
                     /locus_tag="PZ377_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDM6357885.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            6085..7155
                     /locus_tag="PZ377_001001"
     CDS             6085..7155
                     /locus_tag="PZ377_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDM6357886.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            7351..8112
                     /locus_tag="PZ377_001002"
     CDS             7351..8112
                     /locus_tag="PZ377_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDM6357887.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            8130..8624
                     /locus_tag="PZ377_001003"
     CDS             8130..8624
                     /locus_tag="PZ377_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDM6357888.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            8638..9864
                     /gene="pepT"
                     /locus_tag="PZ377_001004"
     CDS             8638..9864
                     /gene="pepT"
                     /locus_tag="PZ377_001004"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDM6357889.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(10053..11177)
                     /locus_tag="PZ377_001005"
     CDS             complement(10053..11177)
                     /locus_tag="PZ377_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDM6357890.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(11602..11922)
                     /gene="ytxJ"
                     /locus_tag="PZ377_001006"
     CDS             complement(11602..11922)
                     /gene="ytxJ"
                     /locus_tag="PZ377_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDM6357891.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(12076..12954)
                     /locus_tag="PZ377_001007"
     CDS             complement(12076..12954)
                     /locus_tag="PZ377_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDM6357892.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            13074..13592
                     /locus_tag="PZ377_001008"
     CDS             13074..13592
                     /locus_tag="PZ377_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDM6357893.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            13719..14642
                     /locus_tag="PZ377_001009"
     CDS             13719..14642
                     /locus_tag="PZ377_001009"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDM6357894.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            14660..14974
                     /locus_tag="PZ377_001010"
     CDS             14660..14974
                     /locus_tag="PZ377_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357895.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(15291..16319)
                     /locus_tag="PZ377_001011"
     CDS             complement(15291..16319)
                     /locus_tag="PZ377_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDM6357896.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(16438..17199)
                     /locus_tag="PZ377_001012"
     CDS             complement(16438..17199)
                     /locus_tag="PZ377_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6357897.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(17196..18152)
                     /locus_tag="PZ377_001013"
     CDS             complement(17196..18152)
                     /locus_tag="PZ377_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDM6357898.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(18139..19110)
                     /locus_tag="PZ377_001014"
     CDS             complement(18139..19110)
                     /locus_tag="PZ377_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6357899.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(19149..19304)
                     /locus_tag="PZ377_001015"
     CDS             complement(19149..19304)
                     /locus_tag="PZ377_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357900.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
BASE COUNT         6512 a         3359 c         2783 g         6870 t
ORIGIN      
        1 attcaaatat atacatccat taacattagc atagtcacta tgtttcattc aacaaattac
       61 attatcgaac tatgaaatag tcataatttg cttttggagt ataaaaaagc acttgtgcaa
      121 aaacacaagt gctttaaact taatttattg ttcactagtt tgaatcaagc catatttacc
      181 gtctttacgg cggtaaacga tacttgttcc atcagtttct ctgtctgtga atacaaagaa
      241 gtcatgacct aatagattca tttgtaatac cgcttcttct gaatccattg gttttaagct
      301 gaattctttt gaacgaataa tttctatctc gttatcatcg taagcgtcat tatcaacttg
      361 tgtttcttgc atttcttgta attcggcaac aaacacttct tgatctcctc gatcacggct
      421 cttacgatta atacgtgttt tatattttcg aacttgtctt tcaagtttat tattaattaa
      481 atcaatacct gcgtataaat catcgtttcg ctcttcagct cttaacgtaa catttttcaa
      541 tggaattgtt acttcaattt tagtagctga atttgaataa gttttaactt taacatgcgc
      601 cactgcattt ggtacgtcat taaaataacg ttccaactta ccaatttttt cctcaatata
      661 gttgcgaata gcatctgtga tagtgaggtt atctccatga atttcaaatc taatcatagt
      721 aatctctcct taaacctctt tattggtaac tctttattat attttaacat ttttacgcta
      781 tcgtgcaaac gcaaacactt tgaattttct gatattttta gcatataatt tacaccctgc
      841 acgatgaatt gttaatccag ttgtataaat atcgtcaacg agtaatattt ccttaccatt
      901 taaatctaat tccgtatcaa taataaatgg attttcatct gccagacgct ctttcttagt
      961 taaatgagac tgttttggtc tatttgacat ctttaaaatc ttatcaaagc gaatcccttt
     1021 agcttttagt actgcttcta ccgggttaaa tgttctagat aaatcatgtg ccggcgaaga
     1081 aggaattggc acaatatagt cataagatgt ttgtggtatt tcaatcaaat gtgccaataa
     1141 ttcacataaa taatagtctt tcaaaaattt atactgatgt atcatctctt tcattaaacc
     1201 gtcatattga aattgacaat ataattgttc cattaaatta aagtgtgccg atagaaactt
     1261 gcagtctaaa caatacgctt catcttgatt taagtgtttt aagcaccttg aacatcgcct
     1321 tgctttaata tcaagtttaa tattgtccca attctctttg catctgtcac ataatctatt
     1381 aggtttcttg aacaaattat aaatggttat attttcatat aacttagcac cacaactcaa
     1441 acaattattc atcaatccaa cctcttttta atgctaattt gttcatcctt tgaatctctt
     1501 ttttagcttg aatcatattc atacttaccc cttcatgaaa aaacaatact tttccagtag
     1561 gacattctaa ttttcgtcca acacgtccag caatttgtat taaagcctct tgagtatatt
     1621 gatgtgcatc gataacaaca acatccaaat ttgccattgt aaatccacgt tctaatattg
     1681 tcgtagtaaa aatgacatcg aaatgtccat tccttaattg ttcaactttt tcaaagcgaa
     1741 aaacatcctc gctatggacg tatgttaatt tagtaatttt ctgcttataa accgaaaatg
     1801 ttttaatcat tgtttctata ttgttaaaaa acaccagtgt ataacgttga ttattaattt
     1861 gatcttgtaa aattcggtat aacattttct gaatcttctt attattaagt ttgaaataac
     1921 gatatttagg aactggaagt gattttttat gaaagcgagc tggcaattta attatatttt
     1981 cgtggggaat ctctgacaga agttgtttcg gtggtgttgc tgtcatataa attgttgcat
     2041 gttcaacttt agaagatgac ttcaatgctt gttgtaaatt tttatccatt gataaaggaa
     2101 aggcatcgac ttcatcaata aaaatagtat caaagtgctg tttgaatcgg taaagttgat
     2161 gcactgtgca tacaacaaaa tgcccttcaa attgttgtct tgattgctgg tgtagtatgt
     2221 ctatatcttc attaagaaat gcgtctttaa tacgtttact aatttctaca acaacatcta
     2281 cacgtggtga cacaatagct atattatctc cctgtattct tgcatattga atgccttgaa
     2341 acatcatttc tgtcttacct gcacctgtta cggcatataa caaaatcgtt tgtctctttc
     2401 taatggctcg aacaatatgt tctgaggcat aagactgctg ttccgacaat tcaaatgaga
     2461 gatgataata tgcttgtgat gaactttgcg aactctctgt tattttatat cttgttacat
     2521 tatccattcg acccagtgat atacaatttc tgcaatatac aattttcttg gaatgtactg
     2581 aggaatcata ctgataaaaa tgctttggat ttgtactttc acattgaaca caataatagt
     2641 ttgaattttg tttgatgaca cctgttactt cttttttaat tttttcatta gtaagtgaag
     2701 tatgaggatg aattaatttc ccgtaatgtt taatgcttaa aacctcattt caacgtaaaa
     2761 aagagcctaa tattataatg attttcttat atctttagca ttttggcttt caaatgatga
     2821 acatttaaag ccatgtatac gagagataat ttccaattac aatatagact cttttattaa
     2881 aattttataa ttatgttaat cttatttttc tgccaacata gcctaaacct aatccaccag
     2941 aacctaaatg tgctgcaaca actggaccaa actcagagta tgctacttga taagctgaag
     3001 gacaatcatc ttgtaacttt ttgtataacg cttgaccatc ttcgaaatga tctccattta
     3061 tgacaaataa agttacttct tcaaagtctt ttacaatatc taatactttc ttttctaatg
     3121 tttgaatggc acgcttttta gtacgaactt tttcttctgg tataatcttg ccatcttcaa
     3181 acttaagaac tggcttcatt ttcaataatg ttccaaccca tgcttgagca ccagtaattc
     3241 gaccactttt ttgtaaattc tttaagtcat caacaattaa atatgcacct gtgtgttcac
     3301 gcatattagt taaatcatca ataatttgct gtggctcgta tccttcttca accatttcaa
     3361 tagcacgtaa tacataacat ccttcaatca ttgctgcaag cttactatca aatgcatgta
     3421 cattaacacc ttcaaccatt tcccctgctt gataactaga ttgataactt ccactaatcc
     3481 cacttgataa gcaaatgaca atgatatctg tgtaaccttg atctcttaac atttcatatt
     3541 tagtaatcca ttcgccaatt gctggttggc ttgttgttgg aatcgtttga gacgatgcca
     3601 ttttattata aaattcttca attgctattt cattactttc tgtaaagttc ttcccatcat
     3661 caaaagtcac acttaatggc gctatttgaa tattatattt atcgattaag tcctgcgaca
     3721 gataacttgt agaatcggtc atcacagcaa ttttcatagt cgtcctccta aaatatactc
     3781 atagcaatga tacaatattt cagttaaaaa gaaaatggca atcaaacgat ttatagtcat
     3841 gtcattttca gtgtataatg aagattaaat aatcttaagc ttatgaatta aggagttatt
     3901 atgacacaaa atatcattac tgttaaaaaa gaacacatta ttgaaaatgt gattagtaaa
     3961 tcaagattca ttgctcatat caaacctgtt caaaatgaag atgaagctaa agcatttatt
     4021 gctgcaataa aaaaagaaca taaagatgcc actcataatt gttctgcata tactattgga
     4081 cctgaaatga atattcaaaa agcgaatgat gatggtgaac cgacaggaac tgccggtgtc
     4141 ccaatgttag acatattaaa aaagctcgat gttcataatg cgtgtgtcgt agtaacaaga
     4201 tattttggtg gtattaaatt aggtggtggc ggtttaattc gcgcctatag cggtgctgtt
     4261 agagacgtca tttatgatat cgggcgtgtc gaactacgag aagcagtacc atgtatagta
     4321 acactgaatt atgatcaaac tggtaaattt gaatatgaat tagcttcaac tcattttatg
     4381 ttaagaaacc aaacttacac tgataaggta agctactata tcgatgtcgt taaaagtgac
     4441 tatgaagcgt ttattaactt tttaaatcaa atgactgcag gaaatttcga tttgactgaa
     4501 gaaactccaa agcagttacc tttcgatata ccaactgaat aaaaataaaa agcactatca
     4561 taagacggta aatatcaatt gcgtattatt aaacgacaat tgaagtacac gtttatggca
     4621 gtgcttttta tatttgaaat ttaatacaaa aaaattgtaa agagtaaagc catataatac
     4681 aaattatccc atacagctat gctttcattc cctattcctc tttatgagat gacttacgac
     4741 taattaaatt taatattggt cggtagttgt tatctattaa tcctgtaaat tcaacaatta
     4801 attcaatact aaatatgatt aatacaaaca ttaatacaac acctaatggt ggcgatacat
     4861 acaaaataat gctcgaaaga ctaaataaaa tagagattga atagattaat aatactgttt
     4921 gtctatgtgt gtagcctaaa gctaatagtt tatgatgcaa atgcgattta tcagcttgca
     4981 ttatatgctg cccttttttc acacgtcgaa tcattgcgaa caaagtatca atgaatggaa
     5041 ctgctaagat aacaattggg aagaacaatg caataattgt aatatttttg aatccgagta
     5101 aagaaaggaa tccgatgata aatccaatca ttaaagcccc actatcacct aaaaatattt
     5161 tggcaggatg gaaattgtaa aataaaaacc caattaaaga gcctaataaa acacaacaaa
     5221 tcatcgttat gaaaatatta gcttgtaaaa ttgcaatgaa ccctattgta atgagtccga
     5281 ttgcagaaac acccgacgcc aaaccatcga gtccatcaat taagttaatt gcatttgtaa
     5341 tagcaacaat ccaaatcaca gtaattggaa tactaagaaa tccaaaatga atcgttgtac
     5401 ccattggcaa cgaaataaaa tcaatagtca caccataaaa agcaactact aaggcagcgg
     5461 caatttgacc agccaatttt atatacggtt tcaaatcgta gatatcatct acaagcccaa
     5521 gtacgtacat aataatcgca ccaataataa gtggtttgat ttctgtttca ataggatgac
     5581 caatccaaat acctattaaa aatgaaaaga gaatcactgt accacccata actgaaatag
     5641 gtttagtgtg tacttttcta aaatttggtt tatcaactaa atttaatctt ttcgatattg
     5701 caataacaat tggtgttatc gtcaaactga caatcattgt tactgcaact agtaataatg
     5761 taaccattta ttcaccttca tcgatattaa ttgtttttaa cgttatattt atttaatatt
     5821 gtctttatag aatgatatat ctattttcta cgtatatctt atagctttat tataaattaa
     5881 ttcgttcaat caatgatatc atatttacaa ctatacgatt catttcttat atatcgccta
     5941 ttattcattt tttcatcata tcatttaagg caacatttta acatcaaagt ctatatcata
     6001 cattaatttt atatgtaaac taatgacaaa ttgtaagttt tcacttacaa tagctagtgg
     6061 tcaattaaag gagtcgattc agacatgttc gaagcattta tatacaatat atctgttata
     6121 gtcgctggaa tctatttatt ccatcgctta cagtattcag aaaacaaacg tatggttttt
     6181 tctaaggctt atgtcactgt tttaatgaca atcgtatcat tattattatc tgtctaccct
     6241 atcccttatc gtgaagatta tttaattcac ttaacattcg ttcctttatt gtttttaggt
     6301 agatttacaa atatggtcta tacattatct gccacagtaa ttgtagcaat tgttgaaatt
     6361 gtagtattta acaactcgat tatgtacggt gttacgttga ttgttattgc tgcagtaaca
     6421 agtgctattg gaccattttt aaaacaaaat gatgtgctat cattattaat acttaatgtt
     6481 gttacaataa tcattttatt tggtgtcgcg ttagtgagtc ctatttacac actatcggaa
     6541 gttatcattt taataccaat ttcattaatt atcactttag cttcagcgat aacatttgtc
     6601 gatatttggc atttcttctc actcgtaaat cgttatgaaa acgaagataa atatgattac
     6661 ttaacaggct taggtaatgt aaaagaattt gatagacatt taaatgaaat ttcacgaaaa
     6721 gctgaaaaag aacatcaaag tatcgcatta ttattaatcg atatcgatgg atttaaagat
     6781 gtcaatgata cctactcaca caaatcaggt gatgctgtat taaaacaaat gtctcaatta
     6841 cttaaaaact atgtgccgaa tcaatttaaa atttttagaa atggtggcga agagttctca
     6901 gttgtcattc acaattattc acttgatcaa agtgttaaat tagctgaaaa tattcgttcg
     6961 ggtgttgaaa aatcatcatt ccatttacca aacaaagaag ttattaaatt gtcagtgtct
     7021 atcggtgttg gttatttaac agatgacgat ccaaaatcac agcgtaaagt gtttaaagat
     7081 gcagacgata tggtgcatgt agccaaaaat caagggcgaa acaaagtaat gtttaaccct
     7141 attatcaatt tataacaacg acaaaactgt taatcctttg ctattattaa atcatagtat
     7201 aatagcaaag attaacagtt tttcttattt aaatttgaac catacaagtt catcatacta
     7261 acacaaagtc tcactattga aagttgtaac aacaagttgt aaacttcaat tgttcaaata
     7321 aataacacat atcattggag gtgaattgat atgccagatt caatcacaat tatagatgaa
     7381 aacaaagtga ttgatgttgt attaattgca ggtagaattt tacttgaatc aggtgctgaa
     7441 acatatcgag ttgaagatac aatgaaccgt atcgcacata gttatggtct tcataataca
     7501 tatagttttg tcagttcaac tgcaattatt ttttcattaa acgatcgaac aagtacaaga
     7561 ttaattcgtg tacaagagcg tacaacagat ttagagaaaa tcgctttaac gaatagtctt
     7621 tcacgtaaaa tatcaaataa agaactcaca attgatgaag ctaaatctga atttattcat
     7681 ttacagcatg catcattaca atattctttt ttaacgaatt tctttgcagc tgccattgca
     7741 tgtggcttct tcctatttat gtttggtggt gtcgcatcag attgttggat tgcagtcatt
     7801 gctggcggat ccgcattttt aacattcagc tttgtgcaac gctatataca aattaaattt
     7861 ttctcagagt ttgttgcagc cgctgtggtc atatccattg cagccacatt tactaaatta
     7921 ggcattgcaa ccaatcaaga tatcattact atagccagtg tcatgccact tgtccctggt
     7981 atattaatta ccaatgccat tcgagattta ttagcgggtg aattacttgc aggtatgtct
     8041 cgcggtgttg aagctgcatt aacagcattc gcaatcggtg ctggtgtcgc aatcgtttta
     8101 ttaatcattt aaggaaggac tgttaaagca tgctatttta tttatttcat tttacaatca
     8161 gctttatatc aacagtactt ttctctatca ttttcaatgc acccaaacgc ctcttagtag
     8221 catgtggatt tgtgggtgcc attgcatgga cgatttacca attaacggta gatttagagt
     8281 ttggaaaagt tggcgcttca tttttgggaa gcttaatttt aggcttaatg agtcatacta
     8341 tgagtcgcag atataaacga ccggtaatta tattcatagt gccaggcatt ataccattag
     8401 tacctggtgg tgcagcttat caagcgactc gttttttagt atcaaatgat tatacaagtg
     8461 ctgtaaatac atttttagaa gttacactga tttcaggtgc gattgctttc ggtatattag
     8521 tttctgaaat tctatattac ctatacacac gtatcaaaca actgtatggt aaaatcaaag
     8581 gtaagacata taaaaaatct tacaacatga ataatagagt ttaaggggtc gaatataatg
     8641 aagaaccaat tgatagatag attaacaaga tatacgacaa ttgatacgca atctgatcca
     8701 aaatctacaa caacaccttc aactgaaaag caatgggatt tgttacattt attagaaaaa
     8761 gaattacaac aattaggatt gccaactgat ttagatgaaa atggctactt atttgctaca
     8821 ttagaaagta acatcgatgt cgacgtccca acagttggat ttttggcgca tgttgataca
     8881 tcacctgatt tcaatgcttc aaatgttaaa ccgcaaatta ttgaaaacta tgatggtaag
     8941 ccatacaaac taggcaatac taaacgtgtc ttagatccta aagtatttcc agaacttaac
     9001 agtttagttg gtcatacatt aatggtaact gatggtacat cgttactagg tgcagacgat
     9061 aaagcgggta ttgtggaaat tatggaagct atttgttatt tacaagaaca tccagaaatt
     9121 aaacatggta ccattcgcat tggatttaca ccagacgaag aaatcggtcg tggtccacat
     9181 aaatttgatg ttgaccgctt caatgctgat tttgcttata ctatggatgg tagtcaatat
     9241 ggagaattac aatatgaaag ctttaacgct gctgaagcgg ttattacatg ccacggtgta
     9301 aatgttcatc ctggttcagc taaaaatgca atggtaaacg caatacgttt aggtgaacaa
     9361 ttcgatagtt tgctacctga tagtgaagtt ccggagcgaa cagaaggata cgaaggcttt
     9421 tatcacttaa tgaactttga aggaactgtt gaaaaagcaa ctttgcaata cattattcgt
     9481 gatcatgata aaaaacaatt cgaattgcgt aagaaacgta ttttagaaat acgtgacgat
     9541 atcaatgccc attttgaaaa ttatccagtt aaagttgata tatcggatca atatttcaat
     9601 atggcagaaa aaatattacc attgcctcat attattgata tacctaaacg tgtctttgcc
     9661 aaattagata ttccagcaaa cactgaacct attcgcggtg gtacagatgg ttcacaattg
     9721 tcatttatgg ggttaccaac tccaaacatc tttacaggtt gcggtaactt ccacggccca
     9781 tatgaatatg catccattga tgttatggaa aaagcagtac aagtaatcat tggaattgta
     9841 gaagatatcg ctgaaaatca ctaaaaacca acgattatat actagttcaa ctcaggctta
     9901 attaatatat aaaaatcacg ctaacattca gtgcaatgat agcgtgattt ttcatatttt
     9961 ttacgtatac tttcgatctt ctaattcgtt ttgctaacca tcataaatgt tattaaccat
    10021 catgacagca tcaccaattt agtccttctt tactaaacct caacattaaa atgtgttttc
    10081 aacagtttta aagactgaac cgtataatgc ctaatttgta accccatttt aaagtcagta
    10141 taagtttctg gcatatcgat aaatgtatta aacattgctg taaccccttg tgattcaaat
    10201 aaatcaaact tttcagcagt tgcacaaatt gcaatggcaa ccttttgatg tttatgacaa
    10261 agttctgcaa tacgcaatgt tgtcgtttct agcaactgat cattttcatt taatccttct
    10321 ccaaaaataa ttaaatccgc ttgttcaact aaattttcta aatgtgttag ttggtctact
    10381 aatgcatgac tggttaatat ttcagcttga tacagtccat tcaagactgc tgcaattcca
    10441 ccaccagcac caccacgttc aactggacca attgcaattt tcaattcact tttaaataac
    10501 tcactaaaat accaaattaa attatcgatt tctgctgctt gattatgatt caactgatac
    10561 gcatcataag tttgcatgat ttcactttgc ttaccatata atcgacttga aaaatccgac
    10621 attacttgaa ttcttgctgt ttccatttta gggtgtaagt tcgacatatc catacgacga
    10681 atatatttaa ttacaccagc accttgtctc atatctacga cacgcccttc gtcatcatag
    10741 aattgagcac ctaatgcttg taacatacct gcaccagcat caaaactatc aatcccacct
    10801 agtgaaatta caacatgttt tgcgtcgtta tctaatgcat gtttaatcat ttctcctaaa
    10861 ccataacttg ttcgttcaac aattggtttt ttacctttta aaaataaatt cccctcgata
    10921 acggtcatcc ctgtatcagt ttgtccgtaa acaccttcaa cttcattcat atctgcatca
    10981 tgtactggta tacgatactt ttgcccagat tgccataaaa atacagaatc taataattca
    11041 tgtcttccat taaacaatgg tacttgaact acatctgcag tttcaatttg gcttgcaact
    11101 gcctcttcaa catatctatt agcttgataa cttgaaataa ttccatgaaa ctcatccatg
    11161 gctactaata ctttcataac cttgaccacc ttactttatt atttgttgtg atacgttttt
    11221 tattcataaa agattaatct taaattttag attttatctc gtgattatat gcgaacattt
    11281 aacatgacat gattattaat tattccagta aataacaata tgcttctttt ggtttagcat
    11341 atatttccta ttaaactaac ttacaaatca tgtttaaatt ttcttattgg attcgtcgtt
    11401 ttccaaaata ttttcaataa aaatactcga atatattata catatgtttg taaaaaagta
    11461 ctaacttaac tgcttctaaa ggtgacataa aataaaaggt cagaacatca ttatagccaa
    11521 actactctga ttcacacgaa tttaagttgt gctattcacg tctagtagtg taaggcaaaa
    11581 tgttccaacc ctatagtttc attattcttc tgcttgtgct aatgacgaca cattgatatc
    11641 accgtggtct cgattccaaa ccatttcacc atttacaaaa taaaatgctt gaggtgattc
    11701 atgtttaacg ttcgtttttt tagcaatata atctgacaaa tcgcgttctt gttggacaat
    11761 caaataataa ccgtccatat cgcgttcata taaaaattta ttaaattgat cgtacgcatt
    11821 tgccgatatt ggacaagttt cactatgttt taatacaaaa acatatttat tttcctcaat
    11881 aacctgttca aattggtcaa ttgaacttag ctttatagcc actctattca cctctaaaaa
    11941 tatttatcac atttaaatag catctgcgtg acatttttat acatatagtt aatatcaatg
    12001 tttaccatta tacacatagt aagttgacat gttaaccata aattcaaaat ttttaacttt
    12061 gaaaaatgat tatagttatt tttcttctaa tttttcaagc tttttttcaa aggctttatc
    12121 gccgtgagtt atatctatac cctttttcgg caacttatct acatcgactt ttgataactt
    12181 ctcactaacc tttattgttt caatacgtgt attataatag ttctgcagac tgtaatacat
    12241 ttctattgcg tttttccttg cattattaac atgcttatca ctaatattag tttggttaat
    12301 atcggtaatt tgcttttcaa tcattggcat aatgtgattt ttaatcgcgc cttttacttc
    12361 attttcttta ctatcatcta ggttcttctt cgcagtatct cttaacgctt tattattatg
    12421 ttctaatttt gtcgtaagat tagttgcttc acttttatta tcagctaatt taatttctga
    12481 ttcaactttg tatctatttt tttcaaattg tttcgtataa tctaaaatgt cttcgttata
    12541 cttgatagat tgattacata aacctatgaa tttttttaat tgatatatcg catccttctt
    12601 ctcatttgca agcgtcatat attctttttt taatttctta actttcattg tatcatcagg
    12661 caaatttttt gctgacttat aatatgcttc aaatttaggt atgagatggt ttttaacatc
    12721 ttcttgtagt gccttaaatt ctttactatt tttatcagtt gtatcagttt tacttagatg
    12781 atcaatttct ttcaaatgta tattatccat gacatgttgt aattcttttt gtttttgctc
    12841 aacatcttta aattgcttag taaattgatg ttgtgcctct ttttctttat taccacaagc
    12901 acttattact acaaataaaa tcgctaaaac agcgataata acaatttttt tcatgattgc
    12961 actccttaac agattcatta tacaaaaaca tgtttattat gaaaatgaat tatgttatag
    13021 tattccagac tttatcgtga ttaaattaat tttcaatatc aaaggagttt atcatgtatt
    13081 caaatgttca accattcatt aatagtgatt taactaattg ttttcaatca caatacactg
    13141 aaattgaatc attattattc aacctcttag acagcccagt aaaatataca aaacatattg
    13201 gcggtacgca tcattttaaa tattcaacag agccaatttt agatatttta gtaggtgtag
    13261 aaaatttgca tgatattaca tccttagatg aaaaacgctt aaattatgta gggttttatc
    13321 gtttgcatca taaatatact aagaaagtta tgatggcgaa gtttaacaat tttcatgaac
    13381 taaaacaaca ggtgcgtcta catatcattc aaatgaatac ccaaacattt gaatcttatc
    13441 ttgcacttga ttacctctta tcaactaatg atgatattgc tttgcaattc aatacaaaaa
    13501 aacaccaaat attgcaacat agcttaacaa ttagacaata tgagatgcaa aagcaacaat
    13561 actttgaaaa actatgtaaa aaatacttat aacgacaata atgccgtatt ttataattat
    13621 actaattcta atactttctt ttcaattttc gcaaatgaat tttaaaattg gtataatact
    13681 atatgatatt aaagacatga gaaaggatgt actgagaagt gataaataaa gacatctatc
    13741 aagctttaca acaacttatc ccaaatgaaa aaattaaagt tgatgaacct ttaaaacgat
    13801 acacttatac taaaacaggt ggtaatgccg acttttacat tacccctact aaaaatgaag
    13861 aagtacaagc agttgttaaa tatgcctatc aaaatgagat tcctgttaca tatttaggaa
    13921 atggctcaaa tattattatc cgtgaaggtg gtattcgcgg tattgtaatt agtttattat
    13981 cactagatca tatcgaagta tctgatgatg cgataatagc cggtagcggc gctgcaatta
    14041 ttgatgtctc acgtgttgct cgtgattacg cacttactgg ccttgaattt gcatgtggta
    14101 ttccaggttc aattggtggt gcagtgtata tgaatgctgg cgcttatggt ggcgaagtta
    14161 aagattgtat agactatgcg ctttgcgtaa acgaacaagg ctcgttaatt aaacttacaa
    14221 caaaagaatt agagttagat tatcgtaata gcattattca aaaagaacac ttagttgtat
    14281 tagaagctgc atttacttta gctcctggta aaatgactga aatacaagct aaaatggatg
    14341 atttaacaga acgtagagaa tctaaacaac ctttagagta tccttcatgt ggtagtgtat
    14401 tccaaagacc gcctggtcat tttgcaggta aattgataca agattctaat ttgcaaggtc
    14461 accgtattgg cggcgttgaa gtttcaacca aacacgctgg ttttatggta aatgtagaca
    14521 atggaactgc tacagattat gaaaacctta ttcattatgt acaaaagacc gtcaaagaaa
    14581 aatttggcat tgaattaaat cgtgaagttc gcattattgg tgaacatcca aaggaatcgt
    14641 aagttaagga gctttgtcta tgcctaaagt ttatggttca ttaatcgata ctgaaacacg
    14701 ctgtcgccat tattttaccg aagaagatat tattgctatt aaatttaaat gttgtaataa
    14761 atactatcca tgctataagt gccataatga gtttgaaaag cacgccatta agcgttggtc
    14821 tgagccttca tttaacgaaa aagcaatctt atgcggtgta tgcaaacacg aattaacaat
    14881 caatgaatat atgatggtag agcgttgtcc aaattgccaa tctcggttta acaatcgctg
    14941 taaatatcac tatcatattt attttgaaat ttaaacaaca tagtcaaaac attataggga
    15001 gtgggtcaga catgataaag caccacttct gtagaattcc tttttgaaat tctctatgtt
    15061 tgggccccgc caacttgcat tgtcagtaga aattgaaaat caaatttctc tatgtttgga
    15121 gcccactagt attgaaatga acttgttaca agtacatgtt cattcaacca acgactgaca
    15181 acagaacatt ttagagtcta ggacattgat tgacgtccca gatttccttt tttaaagaaa
    15241 agaggggagc aatgattaag acctttaacc attgttcccc tcttttaaaa ttattttaca
    15301 actttatcaa gttcctcaat ttgtttaatt gtagttgtag ttgatccagc tgcaaagtac
    15361 cataatttag gatctaaatt atatactttg tcttctttaa ttgctttaac attttttaat
    15421 acaggattat ttaatgcttg tttcgcagtt gatttaccac ttatcgcttg acctctatcc
    15481 atcgctaaaa taacatctgg attttcttta ttaacatatt cgttagaaac attttgtcca
    15541 tgattgctat tacttacttt tttatcaact gcattgaatc ctaatgtatc gtaaactaat
    15601 ccaccaaaac gacctttagg tccaaatgtt gataattcac cttcgttaac tagtaaatac
    15661 ataacagttt tattgaagtt tttcgtttta tctttcattg aagcaatttt gttatctaaa
    15721 tctttattta attctttagc tttatcttct ttatcgtaaa tttttccgat attttcagtg
    15781 ttttgtttca ttgaaccaat taagttcttt tcatctgcac caacataaac aattttcgct
    15841 ttaggtgcag cttttttgaa ttcatctaaa ttcttttgat tagctgtacg tccagagata
    15901 aagattactt cgggtttcgt cgcagcaatt ttatcaaaat tcacttcttt taaattacca
    15961 acgtttgtat atttatcatc tttaaatgat tctaagaaat tcggtaatga cttaccgcct
    16021 tcccctttag gtaatgcttt tactttatct gataagccca tttctttcat tacatctaat
    16081 gcgccatagt ctaacacaac tgcattttta ggattttttg gtacttcaac agtttcttta
    16141 acttttttag cgtcactacc atcttttttc tcgccacgca ttttatagtt attttcaatt
    16201 tttactgtat ctttagaacc tttagtttct gattttgatt gttctttatc agaattgtta
    16261 ccgcatgccg ctaataaaaa cattactgct aatactaaat ataagactgt tttcttcata
    16321 atataatatt ctcctctttt ggcttaatct gtgtgcttga tgtgaaatat aaataaatgc
    16381 tttacgttta acccttcttt aattggcgca tcaccccttt aaagtgtctc caaattatca
    16441 atccttaaaa tataaacaaa tcctttgatt tcttatatgt tcaatttgaa tattcatgtc
    16501 atataaatca cttaaaacac tactttgaat gacattatct ttcaaatctg acttaactag
    16561 ttgtccgttt ttcaatgcta caatctgatc tgaataacag gacgcaaagt taatatcatg
    16621 taacacaatg acaatcgctt tattcatttt atgcgctaac atttttaacg tttgcataat
    16681 ttgaacagca tgcttcatat ctaaattatt taatggttca tctagcaaaa tatattcagt
    16741 atcttgtgct attgtcattg caatgtatgc acgctgacgt tgtccaccag ataatgactt
    16801 aatattacga tttctgatat cttgtagttg caacaaatct agcgcatcat tgacaatatc
    16861 atgatcctct ttcgtcaaac gacctttaga ataagggaat cgtccaaagt ttaccaactg
    16921 ctctaccgta atattcattt cagtatggtt tgtttgtttt aatatagata tttttttcga
    16981 caagtcatta tttttataat cagacatgag ctgtccatct attttcactt caccgttctc
    17041 aaaacgaatt aacctacata tcgctgaaag taaagtactc ttacccgcac cattaggtcc
    17101 aattaaagaa gtcaatttac ctttttcgat atctatgcta atatcttcca atatcatttg
    17161 attatttata gttttagtta aattttcaac ttgaatcatt gcgcatttct ccttctaact
    17221 aatagataaa tgaaataact tccaccaatc aaatcaataa gtatactcat ttctgtcgta
    17281 gcttcaaaca cattttcaac tacccattgc gcactaaata aactaatcca actcaagcaa
    17341 attgtcgcaa ttaaaatata cttatgttca tacgtcttca ttagttcatg cgctagattt
    17401 acagttaata aacctaaaaa tgtaattggt cctactaatg cagttgacac agaaactaaa
    17461 actgagacta ttacaagtag aattcgcgtt aatttttcat acgatatccc aagattaatt
    17521 gcttcagcac gacctaaaag caatacatct aaataaggca atagtaaaat tgtaatgact
    17581 aataagatta ctaatagcac tgctgagaaa gtaactaaat tcgaattaga agcattaaaa
    17641 ttagcaaaca tactactttg tattgctaaa aatgactcag gatccataat cagttgaata
    17701 aaacctgtta tgcttctaaa aaatgttcct aaaaggacac caataagtaa gataaaataa
    17761 attgaaaaat gtccgatttt aaagatacct tggaatagga ttagtgcgaa taacaccatc
    17821 gttataagtg ttaatataaa gttcaaatat acattagtaa cccataccga ttgaattcca
    17881 aataaaaaga ctggcaatac tttgataaat aaataaactg catctaaccc cattattgat
    17941 ggtgtcaata gacggttatt tgtaatcgct tgaaaaatca ccactgaagt tgcaatggca
    18001 gcacctacta aaataattaa tatgaacttt cttaaacgac ttgaaaattg atattcaaat
    18061 atttcaaaat caatacctac aaataagtac agcatactaa ttaataacgt tactgcaatt
    18121 aaaagcatta tttttttatt attgttgcgc ataatttttc ctacctttca taagcaagat
    18181 aaggaaaatg attgttccaa atacacctat tgttaaacca atatttattt catatggata
    18241 aacaacaatt ctgccaacta tatcagaaaa taatacaaag atggcaccta acatcatcgt
    18301 atgagggata gcatttttca aatgatcacc tcgataaatt gaaataatat ttggtattac
    18361 tagtcctaag aacggtaatg ttccaacagt caccactacc aatgctgtaa tagttgcagt
    18421 aataaacaat gcgatgttaa ttaatttttc gtaactcaaa cctaaattat tagtaaagtc
    18481 tttacccatt cctacaatcg tgaaatgatt agcaaaaaga tatgtcaatg ctaaaagagg
    18541 aatacttaaa tataaaattt catagcgtcc acttgtgata atggcaaagt tcccgttaag
    18601 ccagttacca atgctttgaa cagcattcgt gcgcaatgag atgaaggttg tgaaacttgc
    18661 aacaatccca cccatcataa ttcctaaaag cggtacaaaa atgacatctt tcacttttat
    18721 aaaatcaatg attttaacaa ataaaaacgt accgcaaata ctgcaaataa cagcaaatac
    18781 tagttttaat aaaatatgac cggttggaaa gaacaataaa gcaattaaaa tacctagttt
    18841 agcccattcc atcgttccag ctgtagtcgg actaacaaac ttattttgca tcatttgttg
    18901 cattatcaag cctgctaaag ccaacgaact tccagcaatt aaaatactca tcgtccttgg
    18961 tattcggctt gagaataaaa tattccgctg tgactcagtt aaatgtagta aatctttaat
    19021 tgatagttca ctcacaccta tgaacaacga aataattgtc aacaatatta gtaatagaag
    19081 caaaatattt ccctttaata aatacttcat tacaaatttt tctcccttta aattaatgat
    19141 aatcattatc attgataata tttctcatta atagtagcgt tttttgacca taatttcaat
    19201 agcctttcaa ataatttagt gtcaatttta ttgatatttg caattaaact gaaatttaat
    19261 ttttcggaat gtgtattttg tttacttaaa gtaaaataga acacttaaga ttttgatgtc
    19321 tgggaatagt ggaaatgata aaaactacta atgattgatt aagtagtggt tcttaacatt
    19381 agcctcagct aatgtgtact taaaaatagg aatacatgag taaaactcat gtggaagtga
    19441 taatttctaa aggaaaaagt ttttctttat gttggggccc cgcccgaact tgcacaatat
    19501 tgttagctga ctttccgcct gctt
//
LOCUS       DANRDI010000036        19500 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.036, whole genome shotgun sequence.
ACCESSION   DANRDI010000036 DANRDI010000000
VERSION     DANRDI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.036"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(69..233)
                     /locus_tag="PZ377_001016"
     CDS             complement(69..233)
                     /locus_tag="PZ377_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDM6357901.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            complement(226..615)
                     /locus_tag="PZ377_001017"
     CDS             complement(226..615)
                     /locus_tag="PZ377_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDM6357902.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            complement(615..2081)
                     /locus_tag="PZ377_001018"
     CDS             complement(615..2081)
                     /locus_tag="PZ377_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDM6357903.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            complement(2081..3991)
                     /locus_tag="PZ377_001019"
     CDS             complement(2081..3991)
                     /locus_tag="PZ377_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357904.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            complement(4007..4297)
                     /locus_tag="PZ377_001020"
     CDS             complement(4007..4297)
                     /locus_tag="PZ377_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357905.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            complement(4297..5880)
                     /locus_tag="PZ377_001021"
     CDS             complement(4297..5880)
                     /locus_tag="PZ377_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDM6357906.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            complement(5889..6713)
                     /locus_tag="PZ377_001022"
     CDS             complement(5889..6713)
                     /locus_tag="PZ377_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDM6357907.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            complement(6713..12914)
                     /locus_tag="PZ377_001023"
                     /pseudo
     CDS             complement(6713..12914)
                     /locus_tag="PZ377_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            complement(12928..13086)
                     /locus_tag="PZ377_001024"
     CDS             complement(12928..13086)
                     /locus_tag="PZ377_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357908.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            complement(13128..13478)
                     /locus_tag="PZ377_001025"
     CDS             complement(13128..13478)
                     /locus_tag="PZ377_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357909.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            complement(13536..13991)
                     /locus_tag="PZ377_001026"
     CDS             complement(13536..13991)
                     /locus_tag="PZ377_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDM6357910.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            complement(14083..14724)
                     /locus_tag="PZ377_001027"
     CDS             complement(14083..14724)
                     /locus_tag="PZ377_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDM6357911.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            complement(14759..15154)
                     /locus_tag="PZ377_001028"
     CDS             complement(14759..15154)
                     /locus_tag="PZ377_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDM6357912.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            complement(15155..15556)
                     /locus_tag="PZ377_001029"
     CDS             complement(15155..15556)
                     /locus_tag="PZ377_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357913.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            complement(15553..15885)
                     /locus_tag="PZ377_001030"
     CDS             complement(15553..15885)
                     /locus_tag="PZ377_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357914.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            complement(15897..16175)
                     /locus_tag="PZ377_001031"
     CDS             complement(15897..16175)
                     /locus_tag="PZ377_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDM6357915.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            complement(16244..17407)
                     /locus_tag="PZ377_001032"
     CDS             complement(16244..17407)
                     /locus_tag="PZ377_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDM6357916.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            complement(17419..18192)
                     /locus_tag="PZ377_001033"
     CDS             complement(17419..18192)
                     /locus_tag="PZ377_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDM6357917.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            complement(18176..19414)
                     /locus_tag="PZ377_001034"
     CDS             complement(18176..19414)
                     /locus_tag="PZ377_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDM6357918.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
BASE COUNT         5429 a         3949 c         2652 g         7470 t
ORIGIN      
        1 tgtcgtttgg taaatccaaa cattacacca cccactttat tcaaattaaa aagccataag
       61 attataacct atgactctag attttctgga tacttttctc ctgtaataat tgcatattcc
      121 tctttatcta taacttccat atctacatac cacgctatat cttctttact atattctttc
      181 aattgatacc atgttttaat atcttcgaat gttggtgaaa ttaatttaag cattttcagt
      241 ctctccttta acctcttcta attttttatt aagtgtcaca agttgttttg ccattagtgc
      301 attttgctta ttaacttgca tcgataactt tgtactttga acaacttgtt tctgcatact
      361 agcaaccatt tttcgtaaga tgtcatcaga agcgactgtg ttttgttctt cactgtcaat
      421 ctgttgatgc aagtcatctt tttcttctga ataatcttcg ttaaaaacta tttccccatt
      481 tgaatattta aaggctttag gtctaaaaac ttgagagaaa ttttctggta aattttcaat
      541 atcaatacct tcttcaaagc cgccaatgat agcgtatgaa attatctcat tacgcttgtt
      601 aactaatatt tgcattattt tctcactcct ataattttgt taattgtccc tctatttgcg
      661 ttcgcaccag agcctctttg acttcctaag tcgaaataga catcgtttga tatagttaaa
      721 gatgtacgac tagatttagt taatccaaac tcataaacac ctccaccatt tccatcacca
      781 tctggaagat ttgagggatt caatgaaatc tttcctcctc caaaaggact gccaaactct
      841 gtaaagtcac cacctggaaa agtcccataa aaaattaata aaataaattg gtctaaactc
      901 tcatttaagt acaatgtaga gcccacacca tttgctgttc catcaaaaat aaccgaatac
      961 cttttattaa acttgtcatc tgcgtataat ttagcgttac tttcggccat attagctttt
     1021 gattgggcac tttgaacagt ttcaaaaggt gtattgtaat cattaatagc taattctgac
     1081 cactcagacc atgaacccgc ttcttttctt ttaacaaaca ctttatttgt accgttcggt
     1141 cgataagtca tacgcttgta atctgaagtt actactaaat attcgacagt accgttagta
     1201 ctaacatctc ttggataatt tatagcttgc gaaacataaa taaattgggt tgaatcacct
     1261 attctttgtt ctggattatt aaaatcaaat ccagtaatct gcattatctt accatcatct
     1321 ttagtaatct tagctttttg ccaatttgaa gtagaaccac ttgtgactaa accaccacta
     1381 ttcactgact gcttgaaggc ttcatgtttc tcatccatat atcgcttttg ctcatcgaat
     1441 gttcttgaat atgcttgcgc tttattttcc aaatcagata tacggctatt agcaagttgc
     1501 tttaattcat caatacttga agattttgct atttgaatat ctgatagacc tttttcttta
     1561 gctttttcaa tcagactcgc ataatcctca ccatttttta tagcctcgtc cattgctttc
     1621 gcgcgatcca taatagtttt ttctaattct tgaaactcaa caatatagtg tagttttgtt
     1681 tcagagggaa tcttgctaaa caaacttttt tcaacgttaa atgtgatagt tctctctaca
     1741 actaccacgt ctgaattacc taattctgca accgaaactt gagcttgata acttccatct
     1801 cttttaatta catcattagg taattgaaat tttaaaatac ctttaaatgg atctaatatt
     1861 tctagtggag caactaccat tactccttta cctcgaatcg ctattcgtgc tttgatattt
     1921 tcttcactca gtaataacgg ttgattattt ttaatgatat taaaaagaag aacagaagaa
     1981 tcactctctc ctgttctaaa agttatatct agatttgaaa tattttcata atgcgctgtg
     2041 ttttctaaat ttatagctac agatttctct aaattactca ttaacttata attctccctt
     2101 cgtgtaaagt ccatggccct gaacttgttt tactatcata atttttcaat agtatctcag
     2161 cagatgctgt aacactatta cgaactagcc tatgaacaaa gccacctgtg tttgaagctt
     2221 ctacatataa gttccaacca gctacccctt tacgttcagt tggaaaatct gtaaaacgtt
     2281 ttgtatcatc cgtagttaaa taaaacgaca tgcctactat gttaatatct gacatttttg
     2341 tgatgaatga aggtactctc tcccatttac cactattttt aggcacataa ttccagtccg
     2401 aaatgtctcc agttcttcca gaaagcaccc tttcaaaagt catcatattc cttgcataac
     2461 tattacgcgt caatatctga attacatcac cgccagtttg tggtggctta acttccaaga
     2521 accaacctgc atcacgccat tctcttggta atgggaaatc atcgatttga actgtatgat
     2581 cagtgtataa atagtaaaga cctggctctg ttaacatccc aagattctta agtttatcag
     2641 gcctcattgg taaaggttta actctaccac ctgtgtcact catgataaaa ggaacgcctc
     2701 ttgagtgaag tatttctaaa atacctcttt gcccaatcat gaaaatacga tgtgttctat
     2761 ttccatcacc accgacagta acacctagca tcaaagcttt tttaccacta tctttgtcat
     2821 agtatatttg caaaccttct gcttccgcaa attcgccagg aaatgaatct agtgttccac
     2881 catagtcagc attaacctga tacgcttctt ctcctgtttc taaatcgaaa gccgttaaat
     2941 agtttctatt atttggatta ctgtctcctg tataccaata caagtatttt tcatcaaaag
     3001 tcacaccctg cattggttgg gtttcgtttg ttagtctcat agggatactg attttatgca
     3061 aaactttatc aatattttta tcaacatcgt ctaaacttct tatctctata taattcattg
     3121 agttttcaag ttcccactga cttctaggtc tctcaattct gtatagaatt ttattttctt
     3181 tttcatttat gacaggggtg atgtagggtt tttctgggtg tcctgtaaat acatcttgca
     3241 taccatactt gccatagcta atttccacat taggcgtata cttgaaacga actaatgtat
     3301 tctcattatt accatttaag ataaaactat aaatccataa ctcatcatca atatatctat
     3361 aaccgttatg tgtaccatga cccccaccta caatcaatga gctgtctata aattgaccat
     3421 taggtcttaa acgacttagc atatagccat tatttctagc ttgtgtcatg tatactatgc
     3481 ctgttctatt atcaaaccag aaggattgca ttactgcatt tgtaagaggt gcaagttctg
     3541 tcacaaataa aaactcttgc ttatcaggtt caaaacgata ctcgatatca agaatttctt
     3601 gtttggtctt atttaattct cttatagttt cctctttatt aatttgagtt ttggtttccc
     3661 aatcgtctaa atgttctttt aatgtgtcaa aggtttcgcc gtttacatta actcgagctt
     3721 gaacaatctc attagcactg ttattacgtg gtgccacaac aagtgcgtta atttgacttt
     3781 gtaaagattt gtttactgct gcttgcgatc taccattata ataaatttgc tcagcgaagt
     3841 gttgcattgt tttagctttc tgatgcaact taaactctgt tgtcaagcca agcgcaaatt
     3901 gctctattct ttgcaggttt tgaatttctt tagctctata atctcgacct gctaaagctc
     3961 ccaaatcctt tattaaatac aaattttcca taatgcacct tcctttctaa taaaatagca
     4021 ctgtaccaag tttcccacta tcgtcaactg ttattttcca caatttaccg tttggggatt
     4081 tctgtacaat gctattttga ataattcctg cttcgcctat ttttaattta tctaatttat
     4141 ttttatcatc taccgaaatg ataccgtctt gaggcaatcc atcaatatca ctactgcctg
     4201 cataaggtat cccatttata gctttccagt gtgtagctgg aaagtactgt ttatcgtttt
     4261 caagtagcgc tttgatttta acttcttctg ttgccattat attaatacac tccctatatc
     4321 cattgtctcg aaaggagaat tcaaagtact agtgtataaa tgatttatac gatttgcttg
     4381 atagttatat ctattatctt gtgcaataac tcgtctgtta agtgcttgtt gaatttgtac
     4441 catatctttt atttcattgc tgaaagacac ttcatctatt gcgtttacaa atggatgtga
     4501 cctatcaagt ttaacaacct ttaattcagt gttatatccc attaattcat gaacaaaaaa
     4561 tacgctatct cttggctcta ttttttcata acctatataa ttaacatcta attcagtctt
     4621 aggagtatca tttatttgct tttttgcaaa ttctaacagc ttatcctgtg tttcgatatc
     4681 ttcatttgtt tgcgtattag catatcgaat cccaaactgc tttgcactat ctgcgacgta
     4741 gtcgacaatt gctttgtatt gattgcgacc tgaattatca gcaattaaat ttaagactgt
     4801 tgatttttca gttccaacat acatacaagg cttagctttt ttatttgaag atatatcaat
     4861 tctatttttg gggtcttctc ctaaaaatat catttctaaa acgtgcttgc ctttatcaat
     4921 attttttatt aaatctattg tttcagactg aaccgactta gcaaaacaag aaatttgctt
     4981 aatttgcttg ccgtctaaaa tcaacttata tattccacct tgagagccct tttttattgt
     5041 aaatctaact gtttcattac catacttgca atcaaagtta atagtagctt tagacccaat
     5101 tgtttcggta cgataagtac cttcttttat aaaaccattt gaatatttaa tgtcagttgt
     5161 tctaatagga ttataatttt tcttttcctc agctgtatac ttttttccaa aaacttttat
     5221 agctgttctt aattccaatg tactgacagt tgcagataca gtatcagtat tatattgata
     5281 tcgaatcact ttctcgcttc tttgataaaa tgtttcagga gaataaaaac caatctctgt
     5341 atcatttggg taaattatac agccaaacag gtctaccgct tctttacagt attctaagcc
     5401 gtttttgtta cctaattcgt caatcggtac ttttcgctta aaatctccaa ttattttata
     5461 ggtcattttg accgaagttt tttgatttgc aaatccatat cttaagtact catctaaaga
     5521 gtattctggc gttttaccag tttcgctact gtcgtcatca agcttatttg attccactga
     5581 gtgattttga aattcataca ttatgtgata tgccgtaact tcaataaaaa ctttatcacc
     5641 ttcaaccttt ggcgctgtct gcttaattgt atatttttca ccatgataaa ttatgaagtt
     5701 ttcacaaatc aataaatcaa aaacaaaact attatgagta gttctataaa ctgtaaaggt
     5761 gatgtaccta gcttcattca gttcataata ttctttaaaa gaaccataat ctacatctag
     5821 taaattttca caaatcaatt cattaaaatc cattactgat aaatgatcat gataatccat
     5881 taaatcacct acctataaat aaaaggaaac ttaaatgtag ttttaatatc actgacgtct
     5941 cctttaatct taaattcatt tttacctggc gctaatgtta taatgcctct atttgtatca
     6001 attcccactc tatttatatc tcgatatgca tacacaccat ctaaaacaaa atcagtgttt
     6061 ttatctatac ttttgttgta cttaaaaata tcacctgttg tatagttaac cagttcaaat
     6121 cctccactcg catttaaatt aattaatatt ttcaaatcgt gcttgaatcg tggatttatc
     6181 gtatcagtag aaccgttcca aatagtaaat tgatttgatg tatgagtata tttaggtgtg
     6241 aaatcaagag gaattccatt ttcaaacatc caattagagt cgaataagaa ctcgctatcg
     6301 gtccaattaa ctgattcaga atacccttta taaacattta aacttacttc aatttcagtt
     6361 gaagaaccat cttttaaatt agatgtaaca ttagctgtat tcactgcata tttaacacca
     6421 ggcatttgag aagtaataac ataataagga tgtctgcgat taaacacaga tctaaaccaa
     6481 tgctcaaata aatttaaatc tataacatct ataccatcat agccaaacct taatactaat
     6541 gaaaaaggcg caaaactaat tgcgcccggt aaaataccat ctactccgtt aatagttaca
     6601 ctgttatcat tggtgtttgg actttcagcc cttgcatcta aaaatataag ctgattaaaa
     6661 tctgttatta cttcttcctt gtaaccatct atgattttta caaaagattg cattaattag
     6721 tcaaacctcc catataatta tttgcatttg ctctatgccc actttgtttt gacaatattt
     6781 tttctaaacc tctaattgca tcattagaac ctaagttatt atcctgagaa gaaacagttt
     6841 gaatcaatgc atctgttaat ttatttcctt tatcacttaa cataacaatt tgtttcaaca
     6901 atttttcaac tgttgaagta tcattattta cagtgatgtt atttggcttg ccatccatac
     6961 cgatgatgcg cataacctgt tcagttaatt gaattgctcg tttacgtcta gttaaaggga
     7021 taaccatctc ctgtttatct ccttcaccca cttcagcaag ttgatgcttt gtaatcaaac
     7081 caccattcgc atatcttctt ggaccacttg gagaccaacc acctcttggg ttaaactgtg
     7141 agcgccaata tctgttgtta aagaacgcta ataactgatc gtaaccacta tatatattgt
     7201 tgtgacctct aacagcataa tgtctaaatg tttgtgggat atattgaagc aatccttttg
     7261 ctggattgcc ctgtaaaacg ttgatgtctc taagcgcact agattgagtt atacctgcat
     7321 ttcctcctga ttcgtgttga atcaagctaa taatatttcc tacatcaccc gaagtaacat
     7381 taacacccat tcgttttgct gcacgacgta tatcgcctgc ccaagcagat gcagccttat
     7441 taacacctga accacttcga acgccactac ctttaagtga cttcaaccat ttttctggat
     7501 ctttagctgt atcattccct ggatgtgacc cttgcatcaa ttggaaatgt aagtgtgctc
     7561 ctctaacgaa attacctgta gcacctgatt tccctatcag ttgaccagct ttaatacgtt
     7621 ggccttgtct tgctaattgc ttagataaat gcatatacca gttccattca ttagcaccag
     7681 tcttaatttg tatagaatta ccgccaccgt aatcagtcca taccttatct gctataccac
     7741 ctttaacggc ataaacgttt gttccagaag gcataccaaa gtctatacca tagtgacgac
     7801 cgccattaaa gttaagtcca cctgtgtagc gtccaaatct ttgccagatt ggatattcaa
     7861 atagatagct tccatcgcct ccaccaccga aatcttcaaa ccacgatttt actttgtcta
     7921 ctaatttctt tttgagcaat gagtatgcgc ctttagctat ttttactgta gcgttagctc
     7981 cgcctccaaa attaatattt aaacctgaca ttactttatt tactagtttc cctggatgtt
     8041 gtacgtaatc ccacacatcg ccgattttat cgcctaacca agatgcacca tctttgattt
     8101 tatcgcctgc tgcttcaacc atttcttctg cacctttttt gatattatgc gctgtgtttt
     8161 tagctgtagc tccaaattct cctgcttttt tacctatatt acctttaagt tggtctagcc
     8221 aatctttctt tttcgtacct ccatgaaact ttggtaaaac acccatacgc tgtaacttca
     8281 gagtgtcatt agcatttatt acgctatccc caactcctag tggaacaacc acatctcgtc
     8341 cttggggtgc atggaatgtt ccgtcagccc tgtgaattac ttcttgaact ccaccacctg
     8401 gggcgtttcc agaacctcta tcatttaata cagcaaatgt cggttgcgtt aatgctcccg
     8461 aattatcggt agctacaccc tttcctgcta aagtaccagt agacaatgta ggtattggct
     8521 tgatgagatt tttatcagta atggctttag atattttatt aataccgcca atcatgctat
     8581 tcaaaccgcc aatagcttta ttagcaacat ttttacctaa atcagccgca gctcttccca
     8641 tgtctttacc aatatctcta atccaatcat atgttttgga tagccatttt ctaaaaccat
     8701 taaatactga tttagcgtta gaccatgccg aacttgaaat tgcatcaaaa cgatcgtggg
     8761 ctcttgaata catatcccca gtccaatctt ttaaagattt gtatgagtta ctaaaccatt
     8821 tcgatgttcc tttccaaacg gattttgcat tcgaccaagc tgtactagaa atattatccc
     8881 atttcgcgcg agatttatta gccatatccg ttagccagcc ctttgcactt ttatatgcat
     8941 tgctaaacca ttttgatgtg cctctccaaa tagattttga atgcgcccaa gctttatctg
     9001 aagcatctga atacttttgc ttagtttgat tgtaaatact tcctgttgtc gatttaacag
     9061 attgccaagc ttttccaaac catttaccag tactattagc tatagcctta gtgtggtatc
     9121 ctacagaact tttggctgag ctccaacctg aacttaattt gcttggaatc cctttgattc
     9181 cgctccacat ttttttcatt tcgccgccaa aatgattagc atttctgccc attttactaa
     9241 aggcttcgcc agttttactt tttacgccgt cccaagcatt tccaaaccat ttctttatat
     9301 tttctctgtt tctacgagct gtttcttctt gttctttagc gtatttatcg ctttttttct
     9361 tttggtcttc tctaaagtta gaccaccaac ttttaaggcc attccaccac ttttcagtat
     9421 ttttatatac acgtccactg gataaatcca tttctttatc aatatcttta ttttgctttt
     9481 taacaacatc tactacagca tcttttttag attttgcttt tcttacttca tctttatgtc
     9541 tttgatcagc aatagctaac aatttatctt tttcagactt agaaaggttg acgttatttt
     9601 ttatagcaat gacatcatct tcatattgct tgtccacttc tttttttctt gcttttcttg
     9661 ctttttctgc ttctttaatt gctttgctcg cttcgtctat tgaataagca tttctgtttc
     9721 tttgcattct tactaaaata cgctcttgct ctttttcagt cttactcaat tctttaacag
     9781 tgatatcacg tctttgattt tcaagctttt caatttcttt tctttcattt tctgaaatct
     9841 gaccatcact caaagctttt tctttcaatt ctttgatttt ctgattgagt tcttgctctt
     9901 ttttaattcg caagtcattt ttttctttag ttcgagttaa aatgttttgc ttttcttgtt
     9961 catcgaatgc actatactta tcaataagtt cttgagtttt ttcgagttcc tttttatttc
    10021 ttttttctat ttcagctata aggttattag ataaatccgt ttcaattttc aaaagttttt
    10081 ttgctttgtc ttctgatatc tgacccgagt ttaaacgtac tttttccatg attctgctat
    10141 tttcttcaga ataatgcaca tatttttcta aagctttttc tgtttctttt gaaacacctt
    10201 tccctaacac ttttacagta tcagacgctt ttttagaagc tgtgcccatg gtttgcataa
    10261 atcctttaaa cttgttgact cctactttga gaaggtcatc gtcgcttaat gatttataac
    10321 catctttcat atcttttgaa aacttttctt tgaagctttt gccgatactt ccaagatagt
    10381 ttttaaactc tcctagcttt ctaacagcgc cgccaataat tttaccacca aaaaaacttt
    10441 atagtttctc ctaaaccgtt aataccgttt ctgaaccatt ccacacgatc atatgcggtt
    10501 ttaaaaactt tatacgcaat tgtaatagca gttattgtag cacctatagg tcctgttaaa
    10561 aaccttaagg ctacacccgc aaatcttgcg cctccactta ctgcaaataa agattttgca
    10621 gctaatccta aaccgttttt caaaagtttg aacggtaaaa ttgctagctt tgcagaattt
    10681 ttcaaaacat ttataggttt taaattaaac atcatagctc cggctaaccc tttaaagcct
    10741 tttgacgttt ttcctgttgt agaaccaaga aataatgttt gaagacctaa agatttcatt
    10801 gcttttgaat tagtatttga aaggattgta ttttcagcaa tacgtctatt taatgacgca
    10861 tatcctttag cagcacttcc aactgtacgt attaataacc ctccagcaag aactgcaggt
    10921 ccaattgctg caccaaaaag tgctaatcct actgaagctt ttctaaccca accagggaga
    10981 tgtgtaaatc catcaactaa ttttgttaaa ccttccgctc ctgctctaat cataggcgtt
    11041 aaatctttac cgacttcgat tgctaatgat tcaaaagcgc cacctaattg ttccagagcg
    11101 cctttgagat tatctttcat caaatctgct gcttttttac tttcgccatt ggaattcttt
    11161 aaggatttac tatagctatt aattttatct ggtcccgctt caatcaaggc taaaaatcca
    11221 cttgctgctt cagtaccaac tattgtagcc actgtagcta gtttttgttc tctcgtcatg
    11281 cctttcatat tatcttggaa ctgtctaatc aattcaccca tgccaacaaa ttgaccttta
    11341 gcatcagaca aatgaatacc taattttttc atttccttag ctgtattttt acttggatta
    11401 gctagcctga taaatgaagc tcttagggca gtacctgctt gagaaccctc taaacctgag
    11461 ttagataaaa cttcaattgc tgcggaagtg tcctctattg aaactcctaa tgcttttgca
    11521 ggagtaccag catacttcaa tgcatctccc atgtactgaa tatctgcagc actatcattt
    11581 gctgatctcg caagtaaatc agcaacatga tttgcatcag atgcttttaa accgaaagag
    11641 ttaatcgctg aagccattac agttgcagtt gtagccattt ctgcaccact tgcttctgct
    11701 gcactgataa cacctggcat agcctccatt gtttgtttgg cattaaagcc taaagctgcc
    11761 aattcttcca tacctttagc aacttcgtta gcacttttac tggttttagc tcctaagtca
    11821 actgcttgat tagacatgct tttcaagtct ttactgcttg cttgcgcaat cgctccaact
    11881 cgagacattt ggccttcaaa gtctgcactt gtttttaatg ctgcacctaa ccctaaagta
    11941 attggtgtag atacgcccat cgtcattgta cgtcccaggg aagtcatttt gtctccaata
    12001 gaactaaatt tctttgacat gacatccgct tgacttgcaa gtttaccgaa atgactttga
    12061 gctatcattt gttctttgtt aaaagtcttc atttcggatg aagctttatc tattgaacgc
    12121 tccaaattat ttaaagcagc tttttcttta ttaacagctg tttcagcttt tgcgacatta
    12181 gcgctatgat tcttaatagt attgtttaaa tcattaaatt ctttttctgt ttgctttaat
    12241 ttagtattag ttttagcgta agaactttca attttatcat ttgattttga aagattgtca
    12301 ttttgcactt ttagtttttg aacttgattg ccttcttgtt tatattgttc aacaagtgct
    12361 ttatgcttag cggactgctt ctgtactgcg tcacttgctc tttttagttg tgcagtagta
    12421 gcttggttac tattcttaag cttttgttct gcatctctca actgtttaag tttttgatac
    12481 gcatcttgtt tacgttgatt tgtacgttta tattgatttt cagctttttt aagttctgta
    12541 ttcgatgatt ttaaggcttc tttagattta tcaagagcta atttttcttt tttattggct
    12601 tctactaact ttaaatatgc tttctcaaca tcttttacac tggatttagc tttttggtaa
    12661 ttagcgttaa cttgtttaag ctcatcttct acttgagaat acatcttttt ttgaacttta
    12721 agcctatcat ttaacccctt aattctcgcc tgatattttt ccattgattt ttcagactta
    12781 tcaaatgctg acagattagc tttcatttca ctattaacaa cacctaattg tcgctttaaa
    12841 cctttcatgc attcttggac acctaaatgg tctaatttca gctccaaggt catgccttct
    12901 actttttcat tcatattaac ctcctttcta gcttccaaaa agttttctta aatccgtacc
    12961 tgtaatgact ttttgttcac tttgtttttc ttcagtctct tctttattct cttcattaag
    13021 tatttctaaa agttttacat acggctgttt tctgacttca gttaatgtcc acccatactg
    13081 ctccatacag aaacgttgta ttttcttaat gttcgataaa atgtctttta ttgagattgt
    13141 tcttctgtct ttcccatctc ttctggttca gtttctgaat cttcttcatc ttcaccattg
    13201 atttctcgaa atatatcttg taaggctttt gtataagttt tagtactcat cttgttcaga
    13261 acatcttctt cagtcaatcc ttcatcttta aataaatcta ctaataactg tcgctctttt
    13321 tgtctcattt ttgttgcgtt aggtgcttct tttttattct cttgatttac taattctaaa
    13381 tactcatagc atttttctgc ttcgcccatt gttacatctt cttttgtata gctctctgtt
    13441 tttcctgttt tacgatcttt aatttcaaat ttaatcattg tattagctcc ttttattcaa
    13501 ataaaaaaga cgcagatata ctgcgcctta aatccctatc cgtttgttac tgtcactgaa
    13561 atttgtcctg acttatcgct tccatcagta gacatagcag tgattactga agtaccttca
    13621 gctacaccgt gaattgctcc tgtattttca tctacagtaa caaattctgg atgttcactt
    13681 gtatatttca atattttatt cgttgctgtg cttggtgcaa tgtttggctc aacattgtca
    13741 tcggtattta ccataattga tttagtttct ggtgtaaatg atacgcctga gactagaatt
    13801 ggattggttt tgaattgagg tacatcaact ttactagatt ctttaccatt ttcttcccat
    13861 gccacttggt aagtaccttt tggataagtt gtatccgctt ctaaattaga taaagttact
    13921 gacactttgc cttcaccttg ttcagaagct acgacgtcgt ctcctttata aacctttaaa
    13981 gttttagtca taaattattc tcctttgatt tattttgaaa gcccctattc tgctgaaact
    14041 gttgcagatt ttgaattaac tgctacttca acattttggg gattagctgg gtaacgaacc
    14101 tgcagaatcc tctgaatgat cttcactgtc cgtgtatcca acgaatactt ttttgaagaa
    14161 ttctgcttct ccttctttac cttcatgata accgtataca ataccttgtg acgttccatc
    14221 aacatcaact tttctattca tccagtcacc tgttaatttt gtaggttctg gggcttctgc
    14281 tttttcacct cgtgttttaa attcaattga atctaaacta aaagtacctt taagtaaggc
    14341 tacatatacc ggctgacctg ttaaaccatc ttccgattcg ccaattactg ttacatacgg
    14401 tgctcttgta ttctctccta cccaagatgt accattttta tctttagtac gtccaataac
    14461 tgtgtttaaa tcatcacttg gaatattgaa aatactcatg tcagacttaa cttcattagt
    14521 accttgtttt ttcatccata cacgtttgtt agatgcaaac atatctacta aatctggtgc
    14581 taaacctgtg atatttaggt caactgtacc acctttttca tcttcccatg tcatgcgttt
    14641 aactactttt gttgcttctg ggttaaaaac tccaacgtat aatcttttaa aacctacttt
    14701 ataagaacct tgtccttctg ccattgctta tttcctcctt aaaaattaaa aagcacacct
    14761 attcgatgcg ctgattttta taatatatat ttttgggtat gccttgatat cgtctcgaca
    14821 tcacataacg tttagtttct tcaaaataag catctaactg actagatgct tgaattaaat
    14881 tttgttgata taacaggtat cttattcgtt ttgttatatc aattgttttc tgattatttg
    14941 aagattctac atctatttga attaagtatt cttcactgag atatttatca gacataaagt
    15001 ctgaaggcaa atcataaaca ggtgtaataa caacaaaggg tttggaagtt tcagcgtttt
    15061 cagtgacttt gtaatagtat attctagaat ttatatgtgt tttgagctct gcatcagata
    15121 ataaaattcc ttttatggtg tttaatatat tcatttatct ggccaactcc ttttttataa
    15181 tttctctata cttacgttcg ctagcagcta atgtttttgc aataactcca aaacctcttg
    15241 gtgtatattt ttttccatct cttgtataac catgttcatt caagtgaata atgtttttgc
    15301 gattcatagg gcctacccat tcaattaaaa cagccctttc ttgactgcca acttttgtat
    15361 aaggcttaga tttagtcatt tcttctatac tggcacccgt atctttaaag ctctcgaact
    15421 ctttctttaa agcctttata aaaaattcag atgcttcatt taaagcttta tcactcttag
    15481 cctgcattgc ttgtttaccg tataccgatt ctaatttatt caacacttca ggtatccctt
    15541 taatttctac actcattttt ctgataaaac cactgtatta tagccaatat ctggtgtatc
    15601 aattcttatt tctacaatgt tgaataattt atcggaatat aatgcactgt caattttaac
    15661 taagtgattt gtttgtggta gatattcagt tttagaagac ctgacaatta tggttaatcc
    15721 tgattttgat tcagtcgctt ttaaaatttc tctatctttc atagaaggat tataaatttt
    15781 acaaaagcaa ctatacaatt tcattttttc ctcttcatct ggatatggtc ctttgtttat
    15841 atattgaaaa aaatacgcgc gatctttaaa ttcattaaat tccatttaaa aatcacctac
    15901 cactttttta atttcaaaat cattttttgc aatccttttt cattaaacac cttgcttcta
    15961 gattggtcat ttgagtatcc acgactttca taatctcttg caatgatata tttaatcgct
    16021 gtacaaaaaa gcgggtattc caagtcatct ttgtcataat ctggaacccc acttaatagt
    16081 aattcagact tagccgattg aatgagacct tcaattaaat cattttcgaa attatagtca
    16141 attctcaacc acaatttaat ttcttctaaa ctcatttcat cacccctatt cggctgatat
    16201 tacagctgat ttagccttag ctgttacatt aaccttttgg ggcttagctg ggtaatggac
    16261 ctgtattttc ttttgctttt gcaattctga atgcactgtc taatgtacgt tgctgatcat
    16321 accatgctgt taatacaaac aaatattcgc cttttttaac atctttatca gtgtcataag
    16381 ttgttccatc atagttaatt ccaaaataat tgaaatctcc cacaataggt ttaactgctg
    16441 catctgtaaa tactactggt ttgccaaata ctttttctgc tggtgtgtca aagaaatttg
    16501 ttgttccatt tgaaagaaca ctaataattt tgacataatc tgcatatcgc atataaattg
    16561 ttgcgttatc acgataatct tcatgtaaat ctgctaaagc gttaataata gcatcataca
    16621 tgtctgctcc ctcaacttct ttaacagatc cattataaaa tgacatgtgt tctaatccag
    16681 atttaggact tactgctaag gcatctttac gctctttagc tgctaatcct gattgtagtg
    16741 cgttttcaac ccagtttact aaatctacat ctgatccatg aattacagta tctgaaattg
    16801 cagcaaatac tttgaattta ttagtagtga acttgactgt atcacctttt gcttttaatt
    16861 cttttgctgt ttctacgtct gtaatgaaat catcatcgtc taaagtgtat gaaactcttg
    16921 gaatctctaa acctttaatg ttagttagac gagctttttc acgtaattgg tttttagcaa
    16981 atggttctga aacaatttct ttagaaagtg tttttggtaa gagcttatct ccacctgaat
    17041 catttcctgt tggtaaagcg tgtaataaac gttgtgcctc cattgaaggt ttttcaaatt
    17101 catttggtaa aatcgcgtga cgataaaact ctgccttagc tttaaccatc ttctcattat
    17161 cacttaaaga ttgataagct tctcctttat ctttaacttt cgctttttct ttctcttcaa
    17221 tgtcttgcac ttgtctttca acaatgttaa atctttgttg taaacctgct ttttctgttt
    17281 ctagttgttt gatgtcttcc atatcaatat ttggatctgt tgctttctga ctcaattcat
    17341 catttttatt ttttaattgt tgtccaatca tacctaagga ttgttttaat tcatataatg
    17401 tcggcatttc atttcctcct aataattcat tgtcattttt aaaatttcgc attcgcgttt
    17461 aattttttct cttttttctt tttcttctag tgacatactt tctttaggtg tttcaaccaa
    17521 ttcagatgta tctacatcat caattttagt gattttgtct acatctttct ttaaatcttc
    17581 tgggacgttc tcgaaacgct tatattgctc tttagagata ctagcagcta tttcattagc
    17641 tcctaaaatt tcatctatca agccgaaaga caaggcttct tctgcagtaa gccaagtttc
    17701 tgcatctaac atctgtttta agtgttcttg atctaaatct tttgctttat ctaaataagc
    17761 tgaattacta acagcatctg ttttttcaag taaatccgct gtctttctta attcttctgc
    17821 attacctaca gtcataaccc atgaattatg aatcattaaa aaactatttt tgtgcataaa
    17881 aatagtgtca ccactcatag cgataacact agcaattgat gccgctaagg catcgacata
    17941 gatattaatt tttgcaggat gcatttttag catattgtat attgcatgcc cttcaaatac
    18001 actgcctcca gatgaattta tatgaacatc tatttcactg atgtctccta gttcatctag
    18061 tttatttttg aaatctgtag cagttacatc actttcaaac catttatcac ttacaatatc
    18121 accataaata aatatttcac ctttactttt tgattttctt ttcatttgaa aatacttagc
    18181 tttcattgac atttttatca ccacctttca aagattttct taattcaagt ggcgtgtcaa
    18241 ttgggtataa atcaccgctt attagcggct tatctccacc ttcaactggt ggtaaatctt
    18301 cccactctct aatgtcattt atagtgtagt aaccactacg aactgcttta aagtacactt
    18361 ctgcttgtgt tgcactatca gcccttaaat aagatttaac gttaaattta aaatacctat
    18421 ttttttctct gtctgtttta gtaagtagtt tccgattaaa ttcttcttca tactgtttga
    18481 cgattggcaa taaggtatgc tgcaagtaaa atctgtttaa ctcttcattt ttcgcgaaat
    18541 ttgtatttga tcttgcattt aagaatactg agggcaattg aaaaacgtta gctactcttt
    18601 ctcttgttaa attctcgctt gccactatat cttcagagac atatttttta ggtaacggtt
    18661 cgatttcaac accaggctct tggaataata ttccaccgtt ttcttcatag tactgtttga
    18721 aatcttctaa cacttgctgc cttttttctt tacctacatt ggaaccatat ttaagcatga
    18781 aagaatcagg tttttgcatt tctgtaagat taaaggttct tactgcatta tcaaaatcag
    18841 ttgtattctt caacacatca atcggactaa tgccttgcac catattagat gccacgatgt
    18901 gtttaaaatg caacatgtcc atattatgaa caatcaattt atttccagtt gcagcatgaa
    18961 tggaataata aagttcacgt gattggtttt caattaacat ttcaacaaca tctggattta
    19021 ataagaaaag ctttgatggt tgatgataga tgtctcgttc aattagcaca tatgcattac
    19081 ctttttcatt tctgattgtt tcaatttgat taataaaatc aaaactgctc agagaattat
    19141 tcggtgacac tgtaagtaaa tcagatactt ctgtattaac tactttataa tcttcataca
    19201 ttttcaaggg caaactagcc attgaattag ataactttgt aatagctgaa aatatcgttt
    19261 cattagtttc aagcgtatta ttgattacac cccaaaaaga tttatttttc catgggctaa
    19321 agtcataaag cttagaagct gactgatcaa tccaattgtc tatcaatttt ttctttatgc
    19381 gtgtgacaat attctctttt gcgataacat tcacctcctt aacgcattat atctttaata
    19441 ctaataaatt ctatgtttcc ttcacccttg tcagaaacaa ctttattcat aatatctgta
//
LOCUS       DANRDI010000037        19414 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.037, whole genome shotgun sequence.
ACCESSION   DANRDI010000037 DANRDI010000000
VERSION     DANRDI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.037"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            31..846
                     /locus_tag="PZ377_001035"
     CDS             31..846
                     /locus_tag="PZ377_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDM6357919.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            1057..1911
                     /locus_tag="PZ377_001036"
     CDS             1057..1911
                     /locus_tag="PZ377_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDM6357920.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDDTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(1971..4394)
                     /gene="hysA"
                     /locus_tag="PZ377_001037"
     CDS             complement(1971..4394)
                     /gene="hysA"
                     /locus_tag="PZ377_001037"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HDM6357921.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(4391..4558)
                     /locus_tag="PZ377_001038"
     CDS             complement(4391..4558)
                     /locus_tag="PZ377_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357922.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            4802..5218
                     /locus_tag="PZ377_001039"
     CDS             4802..5218
                     /locus_tag="PZ377_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDM6357923.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            5288..6136
                     /locus_tag="PZ377_001040"
     CDS             5288..6136
                     /locus_tag="PZ377_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDM6357924.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            6356..6481
                     /locus_tag="PZ377_001041"
     CDS             6356..6481
                     /locus_tag="PZ377_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357925.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            6743..7350
                     /locus_tag="PZ377_001042"
                     /pseudo
     CDS             6743..7350
                     /locus_tag="PZ377_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
     gene            complement(7443..7604)
                     /locus_tag="PZ377_001043"
     CDS             complement(7443..7604)
                     /locus_tag="PZ377_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357926.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(7641..8381)
                     /locus_tag="PZ377_001044"
     CDS             complement(7641..8381)
                     /locus_tag="PZ377_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDM6357927.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            8587..9378
                     /locus_tag="PZ377_001045"
     CDS             8587..9378
                     /locus_tag="PZ377_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDM6357928.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            9746..10174
                     /gene="lacA"
                     /locus_tag="PZ377_001046"
     CDS             9746..10174
                     /gene="lacA"
                     /locus_tag="PZ377_001046"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDM6357929.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            10190..10705
                     /gene="lacB"
                     /locus_tag="PZ377_001047"
     CDS             10190..10705
                     /gene="lacB"
                     /locus_tag="PZ377_001047"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDM6357930.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            10718..11650
                     /gene="lacC"
                     /locus_tag="PZ377_001048"
     CDS             10718..11650
                     /gene="lacC"
                     /locus_tag="PZ377_001048"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDM6357931.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            11654..12634
                     /gene="lacD"
                     /locus_tag="PZ377_001049"
     CDS             11654..12634
                     /gene="lacD"
                     /locus_tag="PZ377_001049"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDM6357932.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            12656..12967
                     /locus_tag="PZ377_001050"
     CDS             12656..12967
                     /locus_tag="PZ377_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDM6357933.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            12973..14685
                     /locus_tag="PZ377_001051"
     CDS             12973..14685
                     /locus_tag="PZ377_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDM6357934.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            14703..16115
                     /gene="lacG"
                     /locus_tag="PZ377_001052"
     CDS             14703..16115
                     /gene="lacG"
                     /locus_tag="PZ377_001052"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDM6357935.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            16381..17250
                     /locus_tag="PZ377_001053"
     CDS             16381..17250
                     /locus_tag="PZ377_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6357936.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            17533..18534
                     /locus_tag="PZ377_001054"
     CDS             17533..18534
                     /locus_tag="PZ377_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDM6357937.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            18768..>19414
                     /locus_tag="PZ377_001055"
     CDS             18768..>19414
                     /locus_tag="PZ377_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDM6357938.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVD"
BASE COUNT         6889 a         2826 c         3547 g         6152 t
ORIGIN      
        1 aattcattaa aatattatgg gggaatgagt atgtcaaagc gtctattatt gtttgatttt
       61 gatgaaacat attttaaaca taatacaaat gaagaagatt taagtcattt aagagaaatg
      121 gaaaaattgt tggaaaaact aactaataat aatgaagtga tcactgctgt attgacagga
      181 agtacatttc aaagtgtcat ggataaaatg gatcaagtta atatgacgtt taaaccatta
      241 catatctttt ctgatttgag ttctaaaatg ttcacttgga ataatggcga atatgttgaa
      301 tcagaaacat ataaaaagaa agtcttgagc gaaccatttt tatttgaaga tattgaagat
      361 atattacgtc atatttctgc gcaatataat gtcgaattta ttccacaaag agcatttgaa
      421 ggtaatgaaa cacattataa tttttatttt cattcaacag gtaatcacaa caatgatagt
      481 cgtatcctag aagcacttgt cagatacgca aatgaccaaa actatacagc gagatttagt
      541 cgaagcaatc cattagctgg tgatcctgaa aatgcgtatg atattgactt cacaccgtca
      601 aatgctggaa aattatatgc tactcaattt ctgatgagga aatataatat accagtaaaa
      661 tcaatattag gttttggaga tagcggcaat gacgaagcat atttaagcta tcttgaacat
      721 gcatatttga tgtcgaatag tagagatgaa gcattaaagc agaaatttag attaacaaaa
      781 tatccatatt atcaagggat aacactgcat gttaaagaat ttgttgaagg taagtatgat
      841 tattgattag ttgagtgata tgtatacttt ttaagtgcaa atattaatat ttaagggatg
      901 attagaaaat atggtgaatc gactacactt tagctttaaa aaattattta tatgttgatt
      961 tatccaatta agtgtagacg attcaggtag cgcaaaaatg acgcaggaac tggagaaaga
     1021 attgatacca aaattaatat aataggttaa gtatatatga ataaaacaaa ctttggggct
     1081 tatttaaata aaagtaagag caggaaaata tataagattt ttgatcaaaa tttgaagcga
     1141 gagtttagtt attggcagtt taggaaactt ttcaaatctt atacaaggca gtctgatgta
     1201 catgaattaa aagtgtgtac tcaaaatgat gatactattg agcaaatatg gatagatgaa
     1261 gcaaatcaat ttggagtgtc agtgacttgg aaagataata gtattatcgg atttgttatg
     1321 cgtccgttaa ttaattatga tgcgattaag aatgaaacga ctttaaaata cgcagtgccg
     1381 acaacagatt gctatgagat tttttggggt ggagataatg aattatataa ttatcactat
     1441 ttatacccac atcaaaggta tgctattgat ttagttatta aagaaaatca taaaacatat
     1501 aagaatcaag gaaaagtaaa tactgattat ttttgttttg gaaaagatat cattgcgcca
     1561 gcaaatggta cagttgaaaa agtagtgaat ggggttcaag acaacagtat tggaagtacg
     1621 aatgaatcac agtttttagg aaattatatt gtaattaagc acgcagaaaa tgagtatagc
     1681 ttaatagctc atttacacca atattcaatc attgtgaatg aggggcaaaa tgttaaatat
     1741 ggtgatatca ttgggaaggt tgggaattct ggcaattcta ctgaacctca tatacatttt
     1801 caagtaatga atgataagaa tattgaagca tgtacatctt taaaaattcg atttataaat
     1861 aatcgagaac ttatcaaagg ggatgtggtc tgcggattac aagctgaatg atggcgatac
     1921 ttataaaatc tcgacactat aaaaatggta tagtgtcgag attttcttgc ttatttagtt
     1981 aattcaaagt gcacgccgga ttcattagaa gtcgacgtat ttttgtttgt aatagagtaa
     2041 ccggtcattg aaattttaga ttcaatatct gaagcggaat ttgtagattc aggattatag
     2101 aagctacatt cataagtgtt atcatctttc tttttaagta taaacatacc tttggcttta
     2161 acttcgactt tagtgttgtt aatgtcaaaa gtttgagtgc tattactata attaacacca
     2221 gcccaaaccg attcattatc tttcacaacg tggaagtcat cttcttgctt aacgacagtt
     2281 acttcatctt tttttgtctt aaagacatct ttagataagc ctggatacaa cacatatcca
     2341 tatttattgt cagaattaga atgcttttga gttacttcat aatactcatc agttttttgt
     2401 gtatcctttt gacttttatt tatttctttc cacttaccag tatgactttc tttttttaca
     2461 gttattttcg gtttgtttaa aaaatgataa ccgatgttct ttttggtatc tgtggactct
     2521 aaaaagactg aattgttttc ctgattatca gaattggttg tttgtttatc gtctgtatat
     2581 aacgtatacc cattcgcttt gcgattttca atcgttgtaa ctggattctt tgatgaatca
     2641 gtacttttaa tgccagttcc taagaagaca attttatcgt ttaatatgaa atatgatttt
     2701 ttggcagtta aagttttgtc ctgattttca aaatccattc cgatactagc atgttggtca
     2761 tcaacttttg ttccgccaac aaaagtttta ctcgactttt tatcatccgt atcttttaat
     2821 atttcattgt ctaaagttgt tgtacctgat aaacgtttca tatcggctgt cacccagaag
     2881 ttatcatgat agtgtttgac atcgctgtta tataaataag acattccagc accagtgtgc
     2941 caacctttta aattctctcc gttgatactt tcatagcgtg ctacgttttt cgacgtcata
     3001 cttaaaccaa atgcaaagtc taagtctttg ttatgatagg tgacacgatc catgtcatta
     3061 tatattttaa gttgttgtgt taatccgttt ttagaaatac tgttatctgt cattaaagac
     3121 ttcattttat ctatgtccga atatgaattt aaataatcat tttgtttata acttgaatct
     3181 gactctactg aagatttgac aatcttttta tacttagctt ttgttgaatc atccatggca
     3241 tcacttaatc tcaacaatga tttcattact gttgcagatg ctgagtgact ggtttcattt
     3301 tcacgactga tagctctacc tcgtgataaa tccatcattt cacctttata aatgagtggc
     3361 ataaatccgt cgtcaatcca tgactttaag gttgtatcat tttgggtttt atcattaaaa
     3421 ggtgtttctt ttatcatcgg catcatttga gaaatcccct ctaagagtac aacgccataa
     3481 gcaccagtgt atggaacgtc ttgatgatca atgtaagagc catctttata aaatccatta
     3541 cgttctttac cagtggcaga atcttgaacg taagtgaaga ctttattaaa tgaatctata
     3601 gactttttca tcatatcttt atcttcttcg ataatacatt ctaaaagttt caccttagaa
     3661 atgtctacta gatttccgcc tttagcaagt tcagcttttc ctacagaaga taatattttg
     3721 tcactatctg gggcgaaagt tttaataggg gcagtgaatt ttttcttttc ttcatttgaa
     3781 aattgatcat tcagcaatat aagcgtattt gttaatgatt taggtgttcc aatttcataa
     3841 tcccaccaat ttaagttggt attcttgcca gttgttttag taaaattctc acttaattct
     3901 tttacttttt tatctggttc ttttccatat gcatttttat gcagccactc aagggcatct
     3961 ttcactttct tcttattttc gtcagtattt aaagtggttt taggattcct catcgcttct
     4021 gcgattttct caatattacg ataggtacga gtcatatgag aagaattagt ttcaagggtt
     4081 tccgctcctg accacaagta tttcctacca ctttcagttt tcatttcctt gagtaaattc
     4141 gtcgcctctt tctctgtagc atcaaacttc ttcttcatat ctggattatt ctcatcatac
     4201 ttatcataac catagttaac gtccagccat gtgttcctca atttttcata atctggcgtt
     4261 tgaacattcg tatcagccac agcgatttga tgtttatcaa cacttctgaa ttcaccacca
     4321 ttcaaagtaa tcacaccagc cattaataac gtaatggtgg ataatttttg ccatttcttt
     4381 attctatatg tcattgacat gtctcctttt tgtgttgcgc gtgcgcaatg aatattatga
     4441 ttaaataatg attcaatttt tcaaaattcg ttaacgtata caaatgactg tctactgtca
     4501 aacaatccac aaagaatgtt gatgcatata aacaatcgat cacccaaatt ttccgcatca
     4561 cccaaaaatt gcaactaaaa taattttcaa aaaattaaat attatcaatc ttagtgatta
     4621 tatcatttta aatatttaag ttatacaatt tttaaagtaa ttaaaaacga tgaaatgtaa
     4681 aatgttaaaa aagagacaaa tttaaatttg agtaataaaa taagaagtat tcaaacgaag
     4741 atgacaatat ataaagcaac attactttag acgaccaaac ataactgaaa ggatagtcgc
     4801 aatgaaaact aaagaagtcg tagcgctcat gaatatatct caagacactt taagatatta
     4861 tgaaaaggtt ggtgtgattc caccagttaa tcgagatgaa aatggatata gaatatataa
     4921 tgatagtgat ttaaattgga tttatttagt gaaaaatttg cgaaatgcag gcgtcagtat
     4981 tgaatcgctt atcgaatttt gcaggttagc gcagcttcct aaaaatgaaa atattcaagc
     5041 acagcaaaag caaattttaa ataagcaact cgaagaatta aatgaaaact taaagacaat
     5101 tcatgatgtg agagatttac tacaatataa aattgataat tatgataatc atattgctaa
     5161 aatcaatgct agtgataatt atgatgacaa tgttgaacgt ctttgggaga gaaagtaaac
     5221 ctatggcttg ctatagagtt cgctccatgg tttatagtgg acatacaaga taaaggaggt
     5281 tcattttatg aatcatattg aaataagtaa agatgtaaag ataccagtat taggtttcgg
     5341 agtatttcaa attcctcaag aacaaacagc agaagcggtt aaagaagcga ttaaagcggg
     5401 ttatagacat attgatacag cacaaagtta tttaaatgaa actgaagtag gtcaaggtat
     5461 tgaagcttca ggtattgata gaagtgaact attcattacg actaaagtat ggattgaaaa
     5521 tgttaactat gaagatacta tcaaatcaat cgaacgatca ttacaacgat taaatttgga
     5581 ctatcttgat ctagtgctta ttcaccaacc ttacaatgac gtatatggct catggcgtgc
     5641 attagaagag ttaaaagaaa atggtaaaat taaagcaatc ggtgtatcta acttcggcgt
     5701 tgatcgaatt gtagatttag gtattcacaa tcaaattcaa ccacaagtga accaaataga
     5761 aattaatcca ttccatcaac aagaagaaca agttgcagca cttcaacaag aaaatgttgt
     5821 ggtagaagct tgggcaccat ttgcagaagg taaaaatcaa ttattccaaa atcaattgtt
     5881 gcaggccatt gcagataaat acaacaaatc aattgcacaa gttattttac gctggttagt
     5941 agaacgtgac attgttgtac tcgcaaaatc agtgaatcca gaacgtatgg ctcaaaactt
     6001 agatatattt gattttgaac ttacagaaga agataaacaa caaattgcga cattagaaga
     6061 aagtaatagc caatttttct cacatgctga tccagagatg attaaagcac ttacaagcag
     6121 agaattagat gtttaatcat aatataacaa agtaaatcat ttacattaca ctaacctgaa
     6181 acttggatgt taaagtctat gtttcaggtt attttttact cttaaaaagt gaaaggagca
     6241 agcaaataag ttattatata tttttatatt atgtaataac gtaactaagg ttataattag
     6301 ataattaata tgtcttggaa ttaattattt aataagttgt atagcaggtg gcgatatgag
     6361 atatatacac ttttctatta ttacattaat tacaggtatc attatgcata ttacaatgta
     6421 ctttgtacca tttgaatctc ggaaaatgcc actatttatg acgataattt ttacaatcta
     6481 aaagcagtaa agactcataa tataaccaaa aacattaata aaagtttgtt aaatgataag
     6541 gggatgagta ttggtgaatt tactcaaaag gtttaaggga aaaatcctac ctggagagat
     6601 tcaaaaacta aatggactat ttccaaaaat aaagggcaat cacatggtgg ttcttattgg
     6661 aagttaataa ataacaaagg aaaaagaata gcttctttaa ctaaagaagg aaaaatctta
     6721 agggaataag gtgttgtaaa ttatgtgctt tgatataaat aatttggata taaaaaagtt
     6781 gaattttaga aaggtaaaaa acgcaattca tttaagttta agtggcgata aataccagtt
     6841 tttggaggac ggtaaaacta tcaataatac ttatttttag ctgtatatga taatgcaata
     6901 aacatttttg aagatttgtt ttcgccttct gattctataa atttggttca tgtagtatat
     6961 gtttatagtg aaccttataa gaaaacaaat gttataaaaa aatttacatc gttaacaata
     7021 gatgaattgt attactttaa agaatataaa aaggagaagg atgataatat tttgtgcgag
     7081 gagttttcat ataagtgttc cataaaagat ataaaatata aaaaattatt aaaagctata
     7141 tccaatcaag actttaaagg gttagttcca aatatcaatc agaatgataa ttattctgaa
     7201 atatatataa ttaacaatac taaaaactta atatatcatt tatatgatga tagaggtcta
     7261 tggctagctt ttaataataa tgaagattat gcaagatatt ctgaaaaata taatgattta
     7321 atatcggaat ttgataatga agatatctaa ttttttaata ttcgtagctt ttatttttct
     7381 acttatttta tgtttatttt taatcttaca aatgacaaac cattaaaagt agcatcccaa
     7441 catcaaacaa aaaaacaatt catcaaataa aaatcgctac aaaaccaagt cattaaacac
     7501 gcaataatta aaattttcca ctcattataa ttctgaattc caaatgtcga attccgaaaa
     7561 ccaaactcca aattccaaaa acgcaactcc aaaattaaaa gcatttccct accattcggg
     7621 aaatgctttt tacatactga ttactctgtc attaatgatt ttacaacgga aaccatgtcg
     7681 tcatgtatga ccaaagtagc gtcgctatca taaggtgttc gatctttatt gataataatt
     7741 aaattgtcgc ctttaaaatg tgatattaat cctgcggcag gttgtacaac gagtgatgaa
     7801 cctagtacaa caagggtgtc agcatgttca attttattta atgcccttat gatggtaggt
     7861 tgatctaaca tttcaccgta taatacgatg tccggtcgaa tggcaccacc acaattatca
     7921 caatgtttca aagttctatc aataacatct gacttcgtat aagatttatg acatacatta
     7981 caataaaaac gatttaacgt gccatgtaat tcatcaacat gttgacttcc agcgtctgag
     8041 tgcaaaccat cgatattttg cgtgatgaca cctaaagatt gttgattacg ttctaatttt
     8101 gcaatccaat catgaacgat attgggcatc gtatcgacaa atagtaagcg cttatggcag
     8161 aaattgataa aaccttcagg atcatcttct aaataatcac ggcttaacaa gtattctggc
     8221 gaaagcccat cttttgaaat ttcatcaaat aagccaccca ttgaacggaa atctggaacg
     8281 ccacttgcga cagatacacc agcacctgta aaaaatgtaa tacgattcga actatctata
     8341 atatgtttta gtgtctctaa atcatgtttc aagttaaaca ccccatcaaa ataatgaatg
     8401 atttataacg tcttttactt ttattatagt atattcattt tacgattatc aaaagcgcag
     8461 tttaaacagt tctttttaaa aatgaattat acatataatc gaacagttaa attatattag
     8521 tttttacgca atttttatta tagaataagc atgtatatta aaattgatgt ctaaacataa
     8581 aggataatga cccatcgcct aaataaagga ggcaatttta cattgaataa acatgaacgt
     8641 ttggacgaaa ttgctaaact agtgaataaa aagggcacga taagaacgaa tgaaatcgtc
     8701 gaaggtttaa atgtgtctga tatgacagtt cgaagagatt taattgaatt ggaaaataaa
     8761 gggattttaa cgaagattca tggtggtgca cgcagtaatt caacatttca gtataaagaa
     8821 atatcgcata aagaaaaaca tacccgtcaa attgcagaaa aacgatttat agctaaaaaa
     8881 gctgcatcat taattgaaga tggggatact ttgtttttcg gaccaggaac aacagtggaa
     8941 ctattagcag aagaagtcaa tcatcatacg ctcacaatta ttacgaattg tttgccggtg
     9001 tataaaattt tgttagaaaa acaaacagca cattttcgtg tctatttaat tggtggtgaa
     9061 atgcgccata ttacagaagc atttgtaggt gaaatggcga acgccatgtt ggaaaaacta
     9121 agatttagca agatgttctt tagtagtaac gcagtaaata aaggtgctgt gatgacatct
     9181 acattagatg aagcctatac gcaacaactg gcactaagta attcaattga aaaatacttg
     9241 ttaatcgatc atacgaaagt tggcaaagaa gattttacat cattttgtca gttaaacgaa
     9301 ttgactgcag tggtcatgga ctatgaagat gaagaaaaag tagaaacgat taaaacatat
     9361 attgaagtag tcgactaaat taaaagtata actgcattgt taaaatatgt atctatatgc
     9421 tcataagtga atgtgaattt gcattcattt atgggctatt ttttatgcaa atttatgtca
     9481 acgggaacct cgacaaagtt ttactacaat ttcagacgaa tacctaatta aaacaacacc
     9541 acaatatgaa tgttcactta ttgtttgaaa atacgtcata aacaaacata ttataaacat
     9601 attatgttga tttattgttt gtttatgttt ataatttaaa cgtaatcaaa ttgaaagcct
     9661 tttctcaaga ttttcataac ctacaaataa atatgtcaca ttttaagaaa ggtatttcaa
     9721 aattaaagta aaaaggagtc ttattatggc gattattatt ggttcagatg aagctggcaa
     9781 acgattaaaa gaagtcatca aatcatactt attagacaat aaatatgatg ttgttgacgt
     9841 aacagaagga caggaagttg actttgttga tgcaactttg gctgtagcaa aagatgttca
     9901 aagtcaagaa ggtaacttag gtattgttat cgatgcattt ggtgcaggta gctttatggt
     9961 tgctacaaaa atcaaaggca tgattgctgc tgaagtatca gatgaacgct ctggatatat
    10021 gactagaggc cataacaatt caagaatgat cactatggga tctgaaattg ttggagacac
    10081 attagcgaaa aatgtcgtta aaggttttgt tgaaggtaaa tacgatggtg gtagacacca
    10141 aatccgcgta gatatgctta ataaaatgtg ctaaggagga aaaattacta tgaagattgc
    10201 attaggatgc gaccatattg ttacagatac aaaaatgcgt gtatctgaat ttttaaaatc
    10261 aaaaggacat gaagtcattg acgtaggaac atacgatttc acaagaacac attatccaat
    10321 ttttggtaaa aaagttggcg aacaagttgt tagcggtaat gcagacttag gtgtttgtat
    10381 ttgtggaaca ggtgttggta ttaacaatgc tgtaaataaa gtacctggcg ttcgttcagc
    10441 actagtacgt gatatgacat cagcgttata cgctaaagaa gaattaaatg cgaacgttat
    10501 tggcttcggt ggacgtatta taggtgagtt attaatgtgc gatattatcg atgcatttat
    10561 taatgctgaa tataaaccaa ctgaagagaa caaaaaatta atcgctaaaa ttaaacattt
    10621 agaaacaagc aatgcagatc aagctgatcc acatttcttt gatgaattct tagaaaaatg
    10681 ggacagaggc gaataccacg attaagggac gacacatatg attttaactt taacattaaa
    10741 tccatccgtt gatatttcat acccattaac agcattgaaa ttagatgatg ttaatcgtgt
    10801 tcaagaagta agtaaaacag ctggtggtaa aggtttaaat gtaacaagag tacttgcgca
    10861 agtaggtgag ccagtccttg cgagtggttt tattggcggt gaattaggtc aatttattgc
    10921 taaaaaatta gatcatgccg acatcaagca tgcattttat aacattaagg gtgaaacacg
    10981 aaactgcatt gcaattttac atgaagggca acaaacagaa attttagagc agggaccgga
    11041 gattgataat caagaggctg caggatttat taaacatttt gaacaattat tagaaaaagt
    11101 tgaagcagtt gctatttcag gtagcttacc taaaggatta aatcaagatt attatgcaca
    11161 aatcattgaa cgatgccaaa acaaaggtgt gcctgtgata ttagattgct cgggtgcaac
    11221 tttgcaaaca gttttggaaa atccatacaa acctacagtc attaaaccaa acatttctga
    11281 actatatcaa ttgcttaacc agccacttga tgaaagttta gaaagtttga aacaggcagt
    11341 gtcacaaccc ttattcgaag ggattgagtg gattattgtg tctttaggtg cacaaggtgc
    11401 attcgctaag cacaatcata cattctatag agtgaatatt ccgacaataa gtgtattaaa
    11461 tccggttggt tcaggtgatt ctacagttgc tggaattact tcagcaattc ttaatcatga
    11521 aaacgaccac gatttactaa aaaaagccaa tactttaggc atgctgaatg cacaagaagc
    11581 acaaacaggt tatgtcaatt taaataatta tgatgattta ttcaatcaaa ttgaagtttt
    11641 agaggtgtaa aaaatgtcaa aatcgaatca aaaaatcgca tcaattgaac aattaagtaa
    11701 caacgaaggt atcatttctg cattagcatt tgaccaacgt ggtgctttaa aacgcatgat
    11761 ggcaaaacat caaactgaag aaccaactgt agctcaaatt gaacaattaa aagtattagt
    11821 tgcggaagaa ttaacacaat atgcatcttc aattttatta gatcctgaat atggtttacc
    11881 agcttcagat gcacgtaaca aagattgtgg attattactt gcttatgaaa aaactggata
    11941 tgacgtaaac gctaaaggtc gtttacctga ctgtttagta gagtggtctg caaaacgttt
    12001 aaaagaacaa ggcgcaaatg ctgttaaatt cttactttac tatgatgttg atgatgctga
    12061 agaaattaac attcaaaaga aagcatacat tgaaagaatc ggttcagaat gtgttgcaga
    12121 agatattcca ttctttttag aagtattaac atacgatgac aacattcctg ataacggtag
    12181 tgttgaattc gcaaaagtta agcctagaaa ggttaacgaa gcaatgaaat tattctcaga
    12241 accacgcttt aacgttgatg tattgaaagt tgaagtacca gtaaacatga aatatgtaga
    12301 aggctttgct gaaggtgaag tggtttatac aaaagaggaa gcggcacaac actttaaaga
    12361 tcaagatgca gcaactcatt taccatacat ttacttaagt gctggtgttt ctgcggaatt
    12421 attccaagaa actttaaaat ttgctcacga agctggtgct aaattcaacg gggtactttg
    12481 tggccgtgct acatggtctg gtgctgttca agtatacatt gaacaaggtg aagatgctgc
    12541 tcgtgaatgg ttacgtacaa ctggtttcaa aaatattgat gacttaaata aagtattaaa
    12601 agatactgcg acatcttgga aacaaagaaa ataagtttaa aaggggatta atgctatgaa
    12661 tagagaagaa gtccaattat taggttttga aattgttgca tttgcagggg atgcacgttc
    12721 taagttttta gaagcattaa cagcagctca agctggagat tttgcaaaag cagatgcact
    12781 gattgaagaa ggaaacaatt gcattgcgga agcgcataga gcacaaacaa gcctgttggc
    12841 taaagaagcg caaggtgatg atattgcata cagtgtaacg atgatgcatg gtcaagatca
    12901 tttaatgaca acaattttac tgaaagattt aatgaagcat ttattagaat tttataaaag
    12961 agggtgatga caatgatgca aaaattgatc gcacaaattg aaaaagggaa gccatttttc
    13021 gagaaattat cacgtaacat atatttaaga gccatccgtg atggatttat ttctgcaatg
    13081 cctgtcatct tattctcaag tatctttcta ttaattgcat atgtgccaaa tatatttggt
    13141 tttaaatggg ataaaggcat ggaagccatt ttaatgaaac catacaacta tacgatgggg
    13201 cttgtagcat ttttagtagc aggaacgaca gcgaaatctt taacagattc atttaaccgt
    13261 aagctagaaa gtacaaatca aattaacttt atttctacaa tgctagcagc gatgtgtgga
    13321 ttcttgttct tagcttctga tccagccaaa gatggtggat tcttaagtgc atttatggga
    13381 actaaaggtt tattaacagc attcttatca gcatttgtaa ctgttattgt ttataacttc
    13441 tgtgtgaagc gcaatattac aattaaaatg ccgaaagaag taccgccgaa tatttcacaa
    13501 gtatttaagg acttaattcc attttcagcg gtaatcatca ttctttatgc attagattta
    13561 gtcattcgca acagctttaa atcaaatgta gcggaaggta ttttaaaatt attcgaacca
    13621 ttatttacag cagcagatgg atggattggt gtcacaatta tctttggtgc ctttgcatta
    13681 ttctggtttg taggtattca tggtccgtca attgtagagc cagcaattgc agccattaca
    13741 tatgcgaata tcgaagcgaa cttcaagttg cttcaagctg gagaacacgc agataaaatt
    13801 attacatcag gtacacaaat gtttatcgtt acatttggag gtacgggtgc aacattggtc
    13861 gtcccgttca tgtttatgtg gatgacgaaa tctaaacgaa acaaagcgat tggacgtgca
    13921 tcagttgtac caacattctt tggtgtaaac gaaccaattt tatttggtgc accactagta
    13981 ttgaatcctg tgttctttat tccatttgta ttagcaccaa ttgttaacgt atggattttc
    14041 aaacttttcg ttgaagtgtt aggaatgaat agttttagtg tgaatttacc ttggacaaca
    14101 ccaggtccat tgggcattat catgggtaca ggttttggtt tatggtcatt cgtactagct
    14161 attactttga ttgttgtaga tattattatt tactacccat tcctaaaagt ttatgatagt
    14221 gaaattcttg atgaagaaga aggacgtaaa gaaagtaatt cagatttaaa agaaaaagtt
    14281 gcagcaaact ttgatacgaa aaaagctgat tcaattttag cggcaagtgg tgtatcagac
    14341 gatgcagcta aagcttcaaa tatcacagaa caaacaaatg tcttagtact ttgtgccggt
    14401 ggtggtacaa gtggattatt agccaatgca cttaataaag cagctgaaga atatcatgtg
    14461 ccagtgaaag cggcagctgg cggatatggc gcacatatgg acattatgaa agagtaccaa
    14521 ttaatcattt tagcgccaca agttgcgtct aactatgaag atattaagca agatacagat
    14581 agattaggta ttaaattagc gaagactcaa ggtgctgaat atatcaaatt gacacgagat
    14641 ggccaagcag cccttgattt cgttcaacaa caatttgaaa attaagaata ggagtttttc
    14701 atatgactaa aacattacct gaagatttta tttttggtgg agcaacagca gcatatcaag
    14761 cagaaggtgc gacaaatacg gatggtaaag gtcgcgtagc atgggatacg tatttagaag
    14821 aaaactattg gtacactgca gaaccagcga gtgattttta taacagatat ccagtggatt
    14881 tagaattaag tgaaaaattc ggtgttaatg gtatccgaat ttcaattgca tggtctcgta
    14941 tcttcccaaa tggatatggc gaagttaacc ctaaaggtgt tgagtattac cataagttat
    15001 ttgcagagtg tcataaacgt catgttgaac catttgtaac attgcatcat tttgatacac
    15061 cagaagtgtt acacaaagat ggcgattttc taaatcgtaa aacgattgac tactttgtag
    15121 attatgctga atattgtttt aaagaattcc cagaagtaaa atattggact acattcaatg
    15181 aaattggacc tattggtgat ggtcaatatt tagtaggtaa attccctcca ggtattaaat
    15241 atgattttga aaaagtattc caatcacatc ataacatgat ggtggctcat gctagagcag
    15301 taaaattatt taaagatggc ggatacaaag gagaaattgg tgttgtacac gcattgccaa
    15361 caaaatatcc gttcgatcca tcaaatcctg aagatgttcg tgcagcagaa ttagaagata
    15421 tcatccataa taaattcatc ttagatgcaa catatttagg taagtattct cgtgaaacga
    15481 tggaaggtgt gcaacatatc ttatctgtta atggcggtaa attaaacatt acagatgaag
    15541 attatgccat tttagatgca gccaaagatt taaacgactt cttaggtatc aattactaca
    15601 tgagtgattg gatgagaggt tacgatggag aatctgaaat cacgcataat gcgacaggtg
    15661 ataaaggcgg ttctaaatac caacttaaag gtgtcggaca acgtgaattt gatgtcgacg
    15721 tacctcgcac agactgggac tggatgatct atcctcaagg tttatatgat caaatcatgc
    15781 gtgttgttaa agattatcct aactatcata agatttatat cactgaaaat ggtttaggat
    15841 ataaagatga atttattgaa tctgaaaaaa cagttcatga tgatgcacgt attgattatg
    15901 taagacaaca tttgaacgtt attgcagatg ctattataga tggtgcaaat gttaaaggtt
    15961 actttatttg gtcattaatg gatgtgttct cttggtcaaa tggttatgaa aaacgatatg
    16021 gtttattcta tgtcgatttt gagactcaag aacgttatcc taagaaaagt gcttattggt
    16081 acaaagagtt agccgaaaca aaagaaatta aataattata gatattaaaa ggcctatcag
    16141 tcgagtgctg gtaggtcttt atttatagaa agagataaat taaacatgca tatacatatt
    16201 tatatattaa tcaatgggtg acacaccatt gaaagctaaa gaagcaatca attaacaaac
    16261 gttaacggac aactttccaa agtcatctta aaagttaatg atgtattcaa aatcaactaa
    16321 aggacatttt taatcacatc ctttatactt atataaaaga catgaatggg agttaaaagt
    16381 gtgaagaaaa tttttgtaat tattacgaca ttgttagcgg tcgctattat tataggctct
    16441 attattatgg tggtgtttag tcaacgacag gcacaaacat ttaaaataca acaacagcaa
    16501 tttgttaaaa agccaattcc aacattgttc ttacatggat ttggtggtag tgccaattct
    16561 gaaaagttta tggtgaagca agcagaaaaa agaggggtaa ctaaagatat catcactgct
    16621 tacgtttcaa aggatggtgc agtgacattt aaagggaaac taagaaaaga tgcagtgaat
    16681 cctattgtga aaattgaact tgaaaataac agacaaggct atttggataa gaatgctgcg
    16741 tggtttaaaa atgtactgac taagttacaa tctgaataca atttcgataa attcaacttt
    16801 gtcggtcact caatggggaa cttaacattt gcacaatata tgatgacata tggtaacgac
    16861 aagtcactgc cacagttaaa caaacaagtg aatattgcag ggacatttaa tggtgtttta
    16921 aatatgaatg aagatgtcaa tgaaattaca gttgataaag atggtaaacc aagtcgaatg
    16981 aatcaacctt atcagcaatt acgcgtgcta aaagatattt ataaaggtaa aggtatcgaa
    17041 gtgctaaata tttatgggga tttgaaagat ggtacacatt cagacggcag agtgtctaat
    17101 agttcttcga aatcattaaa atatttactg ggaaatagtc cgaaaagtta tcgagaatct
    17161 aaatatgaag gtgagcctgc acaacatagt cagcttcatg agaatgaaaa tgttgctaat
    17221 gagttaatcg acttcttatg gaaaaaataa agcaactgct agtttttctg gggcaaaaaa
    17281 gcttgtaata ttagagcttt tcaaaaatta tctattgttt gagagtttcc ataaaatgtc
    17341 taagtcgtaa cattttaaat ttatgattat agcaaatata atgtggttga agttacatga
    17401 atgaatcagt gcttaagtca aaatataaca actaagaatg tatttgcata cagacatcac
    17461 gaagacacaa ttcaaactta acgcttaagt aacaaataag taaaagtttc aaataaagaa
    17521 aggtgattaa tgatgcaaaa taaacaaatt ctttttaaca aaataccaga aggcatgcct
    17581 caagaggata cattcaaaat tgaagagatt gacacgccta aacttgagag cgatggtgta
    17641 cttgtacaaa cgctgtatat ttcagtggat ccatatatga gaggacgtat gactaaggca
    17701 gattcgtatg tacagccttt tgaaattggt aaaccgattg ttagccatgt cgttgcaaaa
    17761 gtaattgatt cgacactcgc agattataaa aaaggagatg tcgttgtagg tatgttgcct
    17821 tggcgaatca ttaatcatgt ccaagcggat caaattacta aagttccaac gacagatgta
    17881 ccacttgatt tatatttaag tgttttagga atgccaggac aaacagcata tcatggtctg
    17941 cttgatattg gacaacctaa agccggtgat actgttgtgg tttcagctgc atctggtgct
    18001 gtaggttcag ttgtaggtca aattgctaag attaaaggtt gccgtgtagt aggtattgcg
    18061 ggtggcgaca aaaaagtaaa ttatttaaca gagacactgg gctttgacgc tggtattgat
    18121 tataagaaag aggattttgc agaagcgtta gcacaagcag taccagatgg tattgatgtt
    18181 tattttgaaa atgttggtgg aacagttggc gatgaagtat ttaagcattt aaatcgtttt
    18241 gcacgtgtac cagtatgtgg cgcaatttcg tcatataatc atcctgaagc cgatatcgga
    18301 ccacgtattc aaggtacgct tattaagaag caagtaatga tgagaggctt cttggtaagt
    18361 gaattcgcta atgcttttaa agaagctagt gaacagcttg caacatgggt tcaagaaggt
    18421 aaaattcagt cacaagtaac cattgaagat ggttttgaaa atgcgccaca tgcatttaaa
    18481 aatttattta ctggcgataa ctttggtaaa caagttatta aagtaacgga ataataaagt
    18541 atacgtttta acaaactaac tttgttaatt gaccaaaatt taataatttt ttatctaaag
    18601 taaatttgtt cagcaacatg gtagaaataa gtctaaaatt taatacgaaa gtcgtctgta
    18661 attatttata tattcaaatg aaaaaggagt catgatgctg cgatataagc acacatggct
    18721 tctattttgt ataataagaa ataagttcat aaatgaggtg aaacgatatg aaaatgatag
    18781 gatttgagaa gccctttaaa ttggaagaag gaaatttatt taaagtatac gaacaaagaa
    18841 agccaacgcc agaaaacgat gatatcttag tcaaagtaaa tagcattagt gtaaatcctg
    18901 tagatacaaa gcaacgacaa atggaagtca ctcaagcacc tcgtgtactt gggtttgatg
    18961 ctattggtac tgttgaagct atcggaccag acgttacatt atttagtccg ggtgacgttg
    19021 tattttatgc tggatcgcca aatagacaag gatcaaatgc gacatatcaa ttggtgtcag
    19081 aagctatagt tgcaaaagca ccacacaaca taagtgctaa tgaagcggtt agtctaccgc
    19141 taactggtat tacggcatat gaaacgtttt ttgatacatt taaaatttct acgaatcctg
    19201 ctgaaaacga agggaagtct gtcttaatca ttaatggtgc cggtggtgta ggcagtatag
    19261 ccactcaaat tgctaaacgg tatggtttaa ctgtcattac aacagcatca cgacaagaaa
    19321 caacagagtg gtgtgaaaag atgggcgcag atatcgtatt gaatcataaa gaggatttag
    19381 tgcgtcaatt taaagaaatt ccattagtag atta
//
LOCUS       DANRDI010000038        18797 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.038, whole genome shotgun sequence.
ACCESSION   DANRDI010000038 DANRDI010000000
VERSION     DANRDI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.038"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            10..1548
                     /locus_tag="PZ377_001056"
     CDS             10..1548
                     /locus_tag="PZ377_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDM6357939.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            1583..3715
                     /locus_tag="PZ377_001057"
     CDS             1583..3715
                     /locus_tag="PZ377_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDM6357940.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            3727..4161
                     /locus_tag="PZ377_001058"
     CDS             3727..4161
                     /locus_tag="PZ377_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDM6357941.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            4161..5267
                     /locus_tag="PZ377_001059"
     CDS             4161..5267
                     /locus_tag="PZ377_001059"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDM6357942.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(5586..13004)
                     /locus_tag="PZ377_001060"
                     /pseudo
     CDS             complement(5586..13004)
                     /locus_tag="PZ377_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(13281..14636)
                     /locus_tag="PZ377_001061"
     CDS             complement(13281..14636)
                     /locus_tag="PZ377_001061"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDM6357943.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(14663..15595)
                     /locus_tag="PZ377_001062"
     CDS             complement(14663..15595)
                     /locus_tag="PZ377_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDM6357944.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(15597..16406)
                     /locus_tag="PZ377_001063"
     CDS             complement(15597..16406)
                     /locus_tag="PZ377_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDM6357945.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(16595..17503)
                     /gene="rocF"
                     /locus_tag="PZ377_001064"
     CDS             complement(16595..17503)
                     /gene="rocF"
                     /locus_tag="PZ377_001064"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDM6357946.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            17729..>18797
                     /locus_tag="PZ377_001065"
     CDS             17729..>18797
                     /locus_tag="PZ377_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HDM6357947.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKS"
BASE COUNT         5390 a         3259 c         3095 g         7053 t
ORIGIN      
        1 ggtgtaaaca tgtcacaaac tgaagagaaa aaaggaattg gtcgtcgtgt tcaagcattt
       61 ggatcattct taagtagtat gatcatgcca aacattggcg ctttcatcgc ttggggtttt
      121 atcgcggcca tttttataga taatggatgg ttacctaaca aagatttagc tactttagca
      181 ggaccaatga ttacttattt aatcccatta ttgatcgcat ttagtggtgg tcgtttaatt
      241 tatgatttac gtggtggtat catcgcagca acagcaacta tgggggtcat cgttgcatta
      301 cctgatacac caatgttact tggtgcaatg attatgggtc cacttgttgg ttggttaatg
      361 aagaagaccg accaattgat tcaacctaga acgccgcaag gttttgaaat gttatttaat
      421 aacttttctg ctggtatttt aggatttatc atgacgattg caggatttaa aattttagca
      481 ccacttatga aatttattat gcatatttta tcagtggcag ttgaagcatt agtacatgca
      541 cacctacttc cacttgtaag tattttagta gagcctgcaa aaattgtatt tttaaacaat
      601 gcgattaacc atggtgtatt cacaccgctt ggtgcagatc aagctgcaaa agctggtcaa
      661 tcaattttat acacaattga atctaaccct ggaccaggtt taggtatctt acttgcttat
      721 atgatttttg gaaaaggcac tgcaaaagca acgtcatatg gtgctggaat tatccacttc
      781 ttaggtggta ttcatgaaat ttatttccca tatgtattaa tgcgtccttt attatttatc
      841 gctgttattt taggtggaat gactggtgtt gcaacttatc aagcaacagg atttggattt
      901 aaaagtcccg cgtcaccagg ttcatttata gtttactgtt taaatgcacc tagaggtgaa
      961 ttcttgcaca tgttactcgg tgtcttcctt gctgcacttg tatcattcgt tgtagctgct
     1021 ttaattatga agttcactag agaaccaaag caggatttag aagctgcgac agctcaaatg
     1081 gaaaatacta aagggaaaaa atcaagcgtt gcttctaagt tagtatcttc tgataaaaat
     1141 gttaatacag aagaaaatgc tagtggtaat gttagtgaaa catcttcatc agatgatgat
     1201 cctgaagcgc tattggataa ttacaacact gaagatgttg atgcacacaa ttacaataat
     1261 ataaatcatg ttatttttgc ctgcgatgcg ggtatgggtt ctagtgcaat gggtgcaagc
     1321 atgttacgta ataaatttaa aaaggcgggc attaatgata ttacagttac aaatactgcg
     1381 attaatcaat tgccaaaaga tgctcaatta gttatcactc agaaaaaact aactgatcgt
     1441 gctattaaac aaacaccaaa tgccatccat atttcagtgg ataatttcct taattcacca
     1501 agatatgaag aacttttaaa taatctaaaa aaagatgatc aagcataata attaaataaa
     1561 ttaaaaaatg gaggataccg ccatgttatt gagtacacgt gaaaaagaaa tgatagccct
     1621 attgattaag taccacggtc aatatatcac tatacacgac attgctcagc aacttgcggt
     1681 gtcctctcgt actattcacc gtgaattaaa aggtgttgaa gcatatttaa cttcattttc
     1741 attaacttta gaacgcgcaa acaaaaaagg gctacgcatt gctggcacag attctgattt
     1801 aaacgatttg aagcaatcga ttgcacaaca tcaaaccatt gacttatctg ttgaagagca
     1861 gaaagtaatt attatatacg ctttgataca agccaaggag ccagttaaac aatatagttt
     1921 agcgcaagaa attggcgttt ctgtccagac tttagcaaag atgttagatg atttagagct
     1981 tgatttaaat aagtaccaac tatctttatc tcgaaagcgt ggcgaaggca tttacttggt
     2041 aggtactgaa tcaaagaaac gtgaattttt aagtcaatta atggtgaata acttaaatag
     2101 tactagcgtt tattcagtaa ttgaaaatca ttttgtcttt cattcattaa atcaaatcca
     2161 caaagacttt gttgacttag agcgcatttt taatgttgaa agactattaa tggactacct
     2221 aagtgcctta ccctaccaac ttaccgaatc aagttattta actttaactg tccatatcgt
     2281 gctctccatt tcacgtataa aaaatggaga gtatgtcgca ttaaacgatg atatttatga
     2341 ttctgtacaa aacacatttg aacacaaagt agcaagcgaa cttgctgata aacttggtca
     2401 aatatatgac gtcacgttta atcaggcaga aattgctttc attactatcc atttacgtgg
     2461 agctaaacga aaaaatctta atgatacatc attaaataat cgttgtgaag aaaacaaaat
     2521 taaagcgttt gttaacaaag tagaaatgat ttccggtatg acatttgcag atttggatac
     2581 tttagtagat ggactgacgc tacaccttaa tcctgcaatc aatcgtttgc aagctaatat
     2641 cgagacctat aatccgttaa cagacatgat taagttcaaa tatccaagac tatttgaaaa
     2701 tgtaagatta gctttaaatg attgttggcc tgatttgatt tttccagaga gtgaaattgc
     2761 ttttatagtt ttacactttg gtggctcgat taaaaaccaa ggtaatcgat ttttaaacat
     2821 attagtcgtt tgcagcagtg gtatgggaac tagtcgtcta ttatcaactc gtctagatca
     2881 agtttttagt gagattgagc gtattacaca agcatcagtc agcgatttga agtcactaga
     2941 tttaagtcaa tatgatggca ttatttctac tgtgaattta gacatcgact ccccctattt
     3001 aacggtaaac ccattattac cagatagtga tatcagttat gtcgcacagt ttttaaatac
     3061 aaagtctacg ttccaagaga cgcatgataa atcatcaaac atgattgata aggatgatgt
     3121 tcatgttgaa acgaaagatg ttgatggcaa cacatctttt gaaaatgaac aaacttcata
     3181 cttaacttca gttttcgaaa aacatttaag tgacgaaaaa tcagaacaat tattgcatca
     3241 tatgcgttcg ggtttaactt tgcttgattc agtgaaaata gttagtaccg aagttaaaca
     3301 gtggcaaaca tatatcgcag attatctata tcaatgcgat gtaataaacg atccaacgtc
     3361 attcgctgaa ctactagagc aacgattgat tgacaatcca ggatggatat taagtccata
     3421 tcctgttgca ataccacacc taagagacaa tatgattaaa caccctatga ttctaatcac
     3481 agttttagaa gaaccgttaa cattgcctag tattcaaaat gacaatcaaa caattaaata
     3541 tatgatttcc atgtttattt ctgacaatga ttttatggca tcactggtaa gtgacttgtc
     3601 cgaattttta agtttgaaat tagaatctat tgatactttt atggaaaatc cacaggaact
     3661 tgaaacatta ttaagaaaca aatttttaga acgaattaaa aaacaattta tttaggagtg
     3721 tacaaaatga gcgaattatt tagtaatgac aatatctttt taaatgtaaa tgttaacagc
     3781 caaaatgaag caattgaaaa agcaggtaaa gccttagttg atagtggtgc tgtaacagat
     3841 gcttatattc aagcaatgaa agatcgtgag caagtcgtat caacatttat gggaaatggc
     3901 ttagcaattc ctcatggcac agatgaagct aaaacaaatg tgattcactc aggtttaaca
     3961 ttattacaaa tccctgaagg cgttgactgg gatggcgaag tagttaaagt tgtcgtggga
     4021 attgctggta aagatggcga acatttagac ttgttatcta aaattgcaat tacatttagc
     4081 gaagaagaaa atgtggatcg tatcgttcaa gcaaaatctg cagaagaaat taaacaagta
     4141 ttcgaggagg cagatgcata atgaaagcag ttcactttgg tgctggtaac ataggtcgtg
     4201 gtttcattgg ttatattctt gcagacaaca atgttaaagt aacatttgca gacgtcaatg
     4261 aagaaatcat taatgcttta gctcatgatc atcaatacga tgttatttta gctgatgagt
     4321 ctaaaacaac gacgcgcgtg aataatgttg atgcaattaa ttcaatgcaa ccttctgaag
     4381 cgttgaaaca agcaattcta gaagctgata ttattacaac agctgttggt gttaacatac
     4441 tacctattat tgctaaatct tttgcgcctt tcttaaaaga aaaaacaaac catgttaata
     4501 ttgttgcttg tgagaatgct attatggcaa ctgatacatt gaaaaaagca gtacttgata
     4561 ttactggccc tcttggtaac aatattcatt ttgctaactc agcagttgat agaattgtac
     4621 cattacaaaa gaatgaaaat atattagacg ttatggttga gccattttac gaatgggttg
     4681 ttgaaaaaga tgcatggtat ggtccagaac taaaccatat taaatatgtt gatgatttaa
     4741 caccatatat tgagcgtaaa ttattaactg tgaatacagg acatgcatat ttagcgtatg
     4801 ctggtaaatt tgcaggtaaa gctacagttt tagatgcagt tgaagatagt tcaattgaag
     4861 ctggcttacg ccgtgtttta gctgaaacta gtcaatatat tactaatgaa tttgatttta
     4921 ctgaagcgga acaagctgct tatgttgaaa aaataataga tcgtttcaac aattcttatt
     4981 tatctgatga agtaacacgt gtcggacgag gtacattacg taaaattggc cctaaagata
     5041 gaattataaa accattaaca tatctttata ataaagattt agaacgcact ggtttattaa
     5101 atacagctgc attgttattg aagtatgatg atacagcaga ccaagaaact gttgagaaaa
     5161 ataattacat taaagaacac ggtttaaaag cgtttttaag tgaatatgct aaagttgacg
     5221 atggcttagc cgatgaaata attgaagcgt acaattcact ttcataattt attgagcttt
     5281 gtttgaaaca agaagtttcc aacgttattc gttaacaatc agtaataatg tagtagttcc
     5341 cttgaattaa caatattaaa tttctgaaca taaaaaatac tcccttcaac atagacactt
     5401 aacttgtgtt atgtatgaaa ggagtatttt tgcgttaata atttgtttta ttttcgagcc
     5461 acagccacct attcaatggc tattggtcat tactaaaaca aattcatatt aactgttaga
     5521 cttggttact tagtaaggaa tatttcccta tgaaataact agatgttcac attcttgaat
     5581 aaattttatt cttcagtttg ttggtctttc ttagtgaatc ttctaattaa gaatgccata
     5641 cctgcaccta gagctaattc agcatatggt aaatcgtcat tatgtgacat accagtatct
     5701 ggtaaagttt tagcttgttg tttagcttta ttaacttttc cttgttgagc tgattttgtc
     5761 ttagcttggt ggtcgtcagt gttagttaca ttaagcatat cttgattagc actattgctt
     5821 ccatttgaaa ctgtagctgg agatgcattg gcaccgtcgt tttgcgtagc tttattgttt
     5881 gcagctgaac caactgattt ttgcgtatca ttagtatctg ctgttgccgt atcatctttt
     5941 tggctaacat tagttgaagt cattttttct tttgcttcag aagatgcaga tgttgatggt
     6001 ttattcgaaa cttcagtatc agctttgctt ggcgatttat ctgcttcgtt agatgcaacg
     6061 ttagtttcag acttaagttg tcctgcatca gtttgatttg tcgtactttc ttctttatct
     6121 tttgatgtat tagaaggtac atttggttct gttatgtctg ctgaaggcaa tgtttcagtt
     6181 gttgattcaa ccatactttg atttgttgaa tcactaccat ctttttctgc cttagcttta
     6241 ttttcagatt ttggttgtgc aaccttgtca ttagttgatt gagattcagc actattattt
     6301 acttcagcat tttgttttga atcatttaca gatgcattat ctttgctatc agcagatgat
     6361 gctgcttctg tgctcgcagt tgttggagcc gttgctgttg atcctgttgg tgcattctcg
     6421 tttgttgctg tagttgtact attgttattt gttgtgcttt ctgctggcgt tgcattatca
     6481 gtttctgtta caggtttatc agttgtgccg ttattagttg attctacttc tggtttacta
     6541 gttacatcgt tatccattgt cggactgttt gttgatgcat ctacactaga attgttatta
     6601 gcttgcggtt tatcatttgc atcatcagtt gctgatgttg ctgttgtttc acctgttgcc
     6661 gcatcactat tatttggtgt tgtcggagaa gcgtctgctt tgccattagc tgtcgtctca
     6721 gatacgttag gttgtccagt attttctggt gttgcattag catttgaatt tgctgttgca
     6781 tcattattat ctataccatt attagtatca ttagcatctg gatcattctg aggcacaatc
     6841 gcttcaattg caggtatcgt tacattttgt aattcagcaa cttctgcatt tgtttgtgtt
     6901 ttatctaatt tatcagcaaa tctgtcaaaa tatctaccta aatccgtacg tgcaatttct
     6961 ttcgccgatg catctgcatc tgcattttta attatttcta tttgcttgtt aaccacttct
     7021 ctgattgctt ccaaagcatt tttcttaact tcaggattaa tacgttgtgc tttaagttgt
     7081 tcaagcgcac tatttttgac agtagcgatt tctgcatttg tagtttgatc agaaatatct
     7141 tcagttgctt ttgataaaat gtcttctaaa gcattcgtaa acgcttcttt ttcttcagtt
     7201 gtagcatcag cgttgacatt tacacctgct tcaatctggt ctagtgcagt ttctaattct
     7261 tcgatagctt tttgtttttc tgttgagtcg atttgaatgt tatcaaatgc ttcaagtcct
     7321 tgagctttcg ctttttcaac ttcagcagtt gttgttgcat cagtaatacc ttgtttagct
     7381 tgatctgtaa tttgtttaat cattgctaat gcttcagttt tttcttcagc agttaactgg
     7441 tcactttgat caatagattc tttcgtatct tctgctttaa cttcaatagc ttggttcgct
     7501 ttaggtttaa cagtagcatc tacttgaata gcatcaattg ctgctttacc ttgtgttaat
     7561 gcatcatcaa cgtcaccatt atccacacca ttattaatgc tttctaatgc agtttgaaca
     7621 ttttggtcaa cttgcttaat tgcttgttgc ttttcatctt gtgttgcatt agtgtcagct
     7681 gaaatattat ttttcttctg atctgcataa gcatataaat ctgttgtagc tgattctttt
     7741 ttacctgttg gaattgtgta atcgttaata ttatctaagt cattatgaat ttgaacttca
     7801 atgtcatctt tagaagtagc ttgattaaca ttttgatccg cagtttgttt taattcagca
     7861 agtttttgtt tcgcttcagc aatttcactt gaagtcgatg cgttagagtt atccgcttcg
     7921 tttactttag cattatatgc atcttcaatt ttagctaaag catctttttt gtactcacta
     7981 aatgttttaa ctgcattaat tttagctttt ccttctttaa ctgcattatc aacatattca
     8041 tttgttgatg attgatcgac atttgttttc gcttgattta attcagtatc aacttcttgt
     8101 tttgcatcat taatttcttg ttgtgatgca tttggtgttt gttctatttg tgttttctta
     8161 tcagctgcag cttgatctaa ttctttttta cctgctggtt tcttaacagg atttgcatga
     8221 agttgttgaa ctttttgtac tgctgtatct ttagcagtag ttacatcacc tgtagtagtt
     8281 gctgcattaa tattatttaa accttcttca tatgctgctc taactggtcc aatatcgtta
     8341 cctttttctt cattagtagt ctcattatta ttaagtattt cagttatttt attttgcatt
     8401 tcagttagca attccgctct tgcattcgtt ttaacatctg ttgatgcttg aattgcatta
     8461 tcttttgcag tgtttacatc atcgattgac tgtgcatttt caatattttg attaccttgt
     8521 gttaattgtg cgtctacttg ttgatttgct tgttcttttt cttcagtagt cgcatctgca
     8581 gtttgtgcaa taagcgcttt ttgttcgttt gcttttgttg ctaattcatc tttcgcaaca
     8641 tctttaattg ttgtatctgc agtaatacct tgaatatcag caactgcttg atctttaatt
     8701 tgcgtaacat cattagttgt ttgtgcattt aagatatctt gatacgcttt ttctttagct
     8761 tttaaaacta aatcttttgc tgcatttttc tcttcagttg tagcaccagt tgtattatca
     8821 attgcttgat tttgagttgt cacagcttga tcaacatcat ttttagcatt tgatttaacc
     8881 gctgttgctg gttgcgtgct ttgaattgaa ttctttccag cgtctttcgc ctgatctaca
     8941 ccattatcat cagttgcagc tgtaatatta tttttcgcgt ctgtaactgc tgttgctaat
     9001 tgttgaatag ctgcttcttt ttcttctgtt gtagcgttct gatcattatt gataacattt
     9061 gtttgcgttg cttgtaattg atcaatttca tctttagccg cttgtttctt cacaactttt
     9121 ggtgttaccg cattaatcgc tgcttctgca tttgctttag cttcatcaac ttgtgcgtta
     9181 gtagttgctg ctgaaatggc ttgatttgct ttaccatttt cagtatttgc ttcagcatca
     9241 gctgcttgtt tttcttcatc tgttgcatct ggcgtagctt gaatctcttg caatttgtta
     9301 tttaaaattg ctgtgatttc attacgtgca gttgcttttt tattaactgt tggtgttact
     9361 tgatcaatac tattttcacc tgtcgttttc gcttggtcta catcattttg actattagca
     9421 gcttcaatgt tgctatttgc ttgtgtcaca gcattatcta cgtccgcatt agccgctgca
     9481 atttcttcag cagtaatgtc ttgcgtttga gcgattgctg ttttacgttc attcgctttc
     9541 gtagcaattg cttctttcgc attatcttta gttgttgttg ctggctgaat cgcttcaatt
     9601 ttagcaattg ctgctttttt agccgcttca acttccgcat ttgtatgtgc tgcatctatt
     9661 gcggcatcag ctgttgtttt ttcagtttga acttgttatt tagcagctgc tttttcttca
     9721 gttgttgagc cgttatttcc atcaattgct gtttcttgag cttgtacttt atctgcaatt
     9781 gcttgttttg ctgctggttt aacatttgca tcaggtgtaa tggctgcgat tgtagcttca
     9841 tttgtagttt ttgcattatc cacatcattg tttgctgcag cattatctat atcagcgttt
     9901 gcagtaacta ctgcttgatc cactttgtct ttcgctgctt gttgttcttc agtagtcgaa
     9961 tcattcattg cttcaattgc tgttttacgt tcacttgctt tttgagcgat ttccgctttt
    10021 gcatccgatt tcttagttgt ggcagcttga acttgattaa ttgcagcaat actattgtct
    10081 ttagctgttg ttacatcact gtttgtattt gcagcatcaa gatttgttct tgcttcttgc
    10141 tttttagtat ctaattctgt atatgcagct tgtttttctt ctgtagttga agcattgcta
    10201 ttttgaattt cttgtttacg tgtattatat gcgttttcta cttccgtatc agctgcaggt
    10261 ttaacttttg cttgtgtttg aattgcattg attttatcta atacttttga ttttgtatca
    10321 gcaacttcct gttttgattt aacaacttgg atgtcatgta aaccttgctt ttgagctgca
    10381 tctactgctg catcagcttc tgctacttct tcatttgttg catttgaaac tgtagcattt
    10441 tgagcttttc tagctgttgc agcttgttta acttcattat aggcatccat ttttgtttgc
    10501 gtagagtcag taatattttc aatttttgag atttcttcgt ttttaactct ttctaaatct
    10561 tgtgcagtcg ttgtcgcttc aattgcttta acaccagaaa ctttagctgc attaatacgt
    10621 tcaatagctt ccgctacttc ttcatttgtt gtatcaggat ttaaaggtgc ttgatcaatt
    10681 tgtgcttgaa caacttcgtc aaattcttca agagctgctg ctttagctgt tgtagcaggt
    10741 gtagtttgat taatatcatt gattgctttt gttttaatgg cttctacttg tgcatttgtt
    10801 gttgctgcat caatatcttt aattgccttt gtttcaattt taccaatttt atcatttgct
    10861 acatcttttt catcttgtaa tgatgcattt gactttttaa tttgttgttt acgagttgta
    10921 actgcttgga taatatcttg tttcgcttga ggtttaactg ttggtgtaat cacatcttgt
    10981 tctaacactg cgataccatt atctttttca gttgtgacac cttgtgctgt agtttgttta
    11041 ttaatttcag cgattgcatt atttttatca ttgttaattt tagttacaag tgctgcaact
    11101 tcatcttgcg taactttttt actttgttgt gctgctgtaa ccttttcttg agctttagta
    11161 tctaattcag caattgctgc ttgattatca attaatgcag cttgtaattt agttactaaa
    11221 ccatcaatat ccgcttgaga tgcacgattt gcagttttaa catgattcgc atcttcattt
    11281 aaaatagtat ctgcttgttg tttaagttta ttgtattctg caattgatgc tgttgtgtaa
    11341 tgactattat caacttgtga gtttacttgt tgttgcaacg catctttgtt catttcaacc
    11401 gctactgaaa atggatctgc agttagtgta acttctggtt gcgcattatt aattacaaca
    11461 tctgaagcag tacgatatgt taatttttca ttaaaatcaa tatttttagg tgtatcgata
    11521 ttcgcaacat taactttata tgataatttt aaagatttat ctgggaataa aacttcttta
    11581 gtgtgtgtac cacgtgccgt tgtcacacct tggcttgtaa atgtaacttt atttgcattt
    11641 tgatcataat taacagtcat atttttcaat actgtactgt cttcattacc attagggaat
    11701 gttgtagtta atgaattatt aacgtaagtt acaccttgtg gtaatgttac ttcatattta
    11761 aattgatctg cttttaatga agcaccaaaa ttaccattgt taccgatttc agtattaata
    11821 gtatattctt tattgttagt tgctttcggt gctaaatctc tatttttgac atataaatgt
    11881 gatcctgaga atagacctac attatcaatg aatgaatagt atttataacc atctttattt
    11941 gttgtaattc ttgaagcatc agcaactgct gtattgtcag gaataaattg taaatctaaa
    12001 cgatcagcat cagttggtac tttaaataaa tgtgcagtac cagtcttagc aacatttaca
    12061 gttgctaatt cagtattttt cttagcatct ttaataacta aatttgttgc accttgctta
    12121 ctattcgttt gcatagtagt aaagttaata attaattctg aatctggttt tacatttaca
    12181 gtttttgaaa taccgttaaa gttaccatga tctgtagaat catttgcatt cacacgacct
    12241 aatgcagcca cgtttccttt agcttgatag ttttgagggt tattcttatc aaacatatcg
    12301 cttcgtctta attctgagtt aacgaaacca atcttaccgt tgttaattaa tgaataacca
    12361 tttactttat ctgtaacagt tacagttgga tcttgtctat tctcatctgt tgatatggca
    12421 ggatcatcaa atgttaatgt cgtattaata ctgccttcac cagtattgct agcatttgga
    12481 tcttgagttt gtgcgtttgc tgctacaggt gctgctggtt gcgctgctgc tggagcattc
    12541 gctggctgtg tttgatttgc cggtgttgca ttattattag gtgttgcttg gttatttcct
    12601 tgacctgctt ggtttgccgg tgttgcttga tttccaggtt gtgcttgtgc agcgttattc
    12661 ggatcagctt gattaccttg tccagctggt tgtgtatttg gttgtgctgc tcctcctgct
    12721 ggattagcct gtccaccttg gtttgctggt tgtactgctg gttgtccttg gttggcaggt
    12781 gcagctggct gtgctgtagg attagcttga gcaccagcat ttgcgttagg ctgtgtattg
    12841 gcatcagctg gttgtgctgg ttgattttgt gcaggctgat tttgctctgc tgcagacgct
    12901 gttgtcgggt tagtagatat aaaagtaaca gtggcaatta aagctgaaaa aataccgaca
    12961 ttaaattttc tgatactaaa tttttgttgt ctgaataaat tcattaagtc atcctcctgg
    13021 ttgattattc tcgctgttaa atgatttcac ttaatcaact gttaagataa gtagtagcat
    13081 ctgcgttaaa aacacaaagc aactctatct aattaaaatt aattttatca tcattatata
    13141 ttgagtacca gtgtatttta tattacatat tgattacttt gtttttattt tgtttatatc
    13201 attttacgtt tgtactataa attatttcta caaacacaaa aaaccgatgc atacgcatcg
    13261 gctcatttgt aatacagtat ttatttatct aatcccattt tatcttgaac cacatcagct
    13321 atttgttgtg caaatctttc agcatcttca tcagttgctg cttcaaccat gacacgaact
    13381 aatggttctg ttccagaagg tcttactaaa attcgacctt ctccattcat ttctacttct
    13441 actttagtca taacttcttt aacgtcaaca ttttcttcaa cacgatattt atctgttacg
    13501 cgtacgttaa ttaatgattg tggatatttt ttcatttgtc cagctaattc acttagtgat
    13561 ttaccagtca tttttattac agaagctaat tgaataccag ttaataaacc atcaccagtt
    13621 gtattgtaat ccatcataac gatatgtcca gattgttctc cacctaagtt ataattaccg
    13681 cgacgcattt cttctactac atatctgtcg ccaactttag ttttattaga tttaattcct
    13741 tcttgttcaa gcgctttgta aaaacctaaa ttactcataa cagtagaaac aatcatgtca
    13801 ttattcaatt cttgattttt atgcatttct tgaccaataa taaacataat ttggtcaccg
    13861 tcaacgattt gaccattctc atctactgct atgattctgt ctccatcgcc gtcaaatgct
    13921 aacccaaaat cactttcagt ttcaactact ttttcagcta atttttcagg atgtgtagag
    13981 ccacatttct cattgatatt atatccatca ggactacatc caattgtttc agtatctgct
    14041 tctaagtcac caaataagaa tggcgctagt gatgatgttg aaccatttgc accatctaaa
    14101 gcaattttca aaccttcaaa gttaacatct actgttgatt ttaaatagct caaatatttt
    14161 tgtgcccctt caaagtaatc tgaataatgt acaatatcat tgccaactgg tcttggtaat
    14221 tctgggtttt cttgatccaa taatgcttca atttcatttt cttgttcatc tgatagttta
    14281 aaaccatctg atccaaagaa tttaatacca ttatctgcaa ctggattatg agaggctgaa
    14341 atcattacac ctaactctgc acccatatcg cgtgttaaat atgcaacacc tggtgttgaa
    14401 ataataccta atcgcatcac ttctgcacca attgaaatca aaccagctat taatgctgat
    14461 tctaacattt cacctgaaac tctagtatcg cgacctacaa gtacacgtgg gtgtttttca
    14521 cctttattat gtgctagaac atagccaccg tatcttccta atttaaatgc caattcaggt
    14581 gttagttctt ggtttgcgac acctcttact ccgtctgtac caaaatattt tcccattgtt
    14641 tactctcctt taatttagct atttatttta catttatata agccttcgtt tcacttggtt
    14701 gtgctttagt gacatgttct ggtaaattga tttttacagt cttttcagtt gattctgaaa
    14761 taccatctaa atctacttct gcatcaactt cgcttatatt ttgtaagtca tctcgactac
    14821 cgaagatttc aatttcttta tcttctaaat caatcgaact taactcttta tcatctgcta
    14881 aactaccttt ctgtttaaca tttactttaa cctttttgct aaaaggctct actttaactt
    14941 gtaaattcac ttcattaggt tgaatcgata catttaattt attcagtttt ttatcaaaag
    15001 ccgttacttc tgcgacatct tttgtgtcac cattaatctt tttattagtt ttaaaagtgg
    15061 cttttaaata agcgatatca ttcaattgtt cttctccacc tgttacttta actgtttgtg
    15121 gtgaaacttc ttgctttgta attttataaa gtggatcaat atcactctga cttacatcag
    15181 gttgaactgt catcttttta gttactttgt tttcaagcgt aatatttgct aatttaggtt
    15241 ttacagaata atgaatgtca tcagctaacc ctttaacttg atacttctct tgatattttc
    15301 cagctttagc atttgataaa tcaatcacta ctcttaaatc ttctggattt tcaattttta
    15361 ttatctttga ttgtggtcct gaaatagtca cattaactgt ttcaggcgct tttgttaaat
    15421 gcaaatcttt agtgttataa agaatttcaa cgggtacatc ttgaatcgtt ttactagact
    15481 tttgaccaag attaccagtg ttaaagatat ttccaaaaac attgttaaca gataaaaaga
    15541 aaaacaatgc caaaagaaag gcaataaatc tcaagcccca tttactttct agcatattat
    15601 ttcacacctt tcttttgaaa gcgtgtgcca aaccaatgtt cagcaagcaa ctcttcaaaa
    15661 atttcgtttg aaatgtctcg tcgtaatttt ccatcaaatg ttaccgaaat atcaccagtt
    15721 tcttcagata caataacggt aaatgcatca gatacttctg aaataccaac cgcagctcta
    15781 tgtcttgtac ccaaactttt agatatctta ggactatcag acaatggcaa ataacttgct
    15841 gctgctgcaa tcttcgtgcc ttgaataatc attgcaccat catgtaaagg tgtgttaggt
    15901 ataaagacat taattaaaag ttcttgcgaa atatttgaat ccattgcaat acctgtttca
    15961 atataatctt gaagacctgt ttctttttca aagacaatta atgcacctat acgtctttta
    16021 gccatatatt gcacagcctt tgaaaccgat tgaatcaatt tctcttcatc tttactatac
    16081 gtattagaag tatagcgttt taaaaagcta cctctaccaa gttgttctaa cgcacgtcta
    16141 atttctggtt ggaatattac tattaaagct aatacccccc attgaataac gatatcgaat
    16201 aatttagatg ttgcagtcaa gttcaatatc atacttatct gctgaccaat aacaattact
    16261 aatatccctt taagtaattg tatcgcttta gttcccttaa agaccgtgat gagaagataa
    16321 agtacatacc aaactatcag taaatcaagg atactcgtta caatttttaa cgtactgagg
    16381 ttttgaaaaa agttggaaaa atccataaca tctcctccgg gtaatatttt tccataatac
    16441 ccattatacc aatcatttac atcactgtca tacgaacata tatttaaata gaaaaaattt
    16501 attttaaaga ttataactac tcttaatcat tttagtgaat taaaaaaagt agtgcaaaaa
    16561 gcaaaatata ctttatacac tacaaatcat ttatttataa taaagtttca ccaaaaaatg
    16621 ttccaactaa tgaaaccgct tgttcagcag tatgattatt actgtcaatc aatggattta
    16681 cttcaactaa atccattgag gaaattaaat gtgattgatg cagtaattcc aatgcaaaat
    16741 ggctttctct ataactaaga ccacccaaaa ctctagtacc agtgcctggc gtttcaagcg
    16801 gatctaaagc atcaacatct aaagataaat gaacgccatc aacattgcgt gacttcaaat
    16861 attctattgt attttcaatt acttccttta tccccaattt atcaatatct gacatagtaa
    16921 atgttttaat attatgatct ttgataaatt gtctttcacc tttatctaaa tctctcatac
    16981 caattagtac gatgttttct ggcttgatta cattactatt taattctaaa agttctttgg
    17041 gaccttcgcc tgtcaaaatc cttagaggca taccatgaat atttccactt ggtgactctt
    17101 caggtatatt taaatcacca tgtgcatcat accaaataac acctaaatta ttataatgtt
    17161 tacttattgc tgatactgaa cctaccgcaa tagaatgatc tccaccaaga actagaggaa
    17221 atctgttatt ttcaattgat gctgaaacct ctttatttaa tttttgatta acatctataa
    17281 tttcatcata atttcttaat cctttttgtt cactatgaaa tttttcaatg ttcacagcag
    17341 gtaccttaat atcccccttg tcatatacat caaggtctaa ttgctttaat cttgaaatta
    17401 atccagcata tctaattgct gttggtccta aatcaacacc taattttctt tgtccaaatg
    17461 ttgatggtgc acctataata tcaattgctt ttgtctttgt cataagcgtc cccctttgct
    17521 ctatgtaatt aaagaataat gtatgcgctt accattatca agcaatagct acacatataa
    17581 tctgtttatc ttattacttc ataaaaaaag gttcttcatc ttttatggtg ggaaggtaaa
    17641 acttcctgct ttttttaata cacaaaaagc gcaattgcct ctataattta aagtgaccaa
    17701 acccaaacta aaggagacaa gtgcgcctat gtgtaatgat accttagaat tactaagaat
    17761 aaaagatgaa aatataaaat atataaacca agaaattgac gtcattatca aaggaaaaaa
    17821 agcaacagtg gttaatgctg tactaacgta taagccttcg gcctgttatt gttgtggagt
    17881 taaaaatgaa ggacaaattc ataaacatgg taagcgtgtt tctcgtatta ctttacttaa
    17941 aactcaaggg tataacacat acctcaactt agctaaacaa cgttttaaat gcttagaatg
    18001 caatggcact tttactgcta aaacgtcaat tgttgatgag tcgtgtttta tctcaagatg
    18061 tgttactcaa aaagttatag aagaagctac taaagttaaa acagagattg atactgcaga
    18121 agataactgt atctctccat ctactgtaag tcgtattaga actaaagcgg ctaattcatt
    18181 acgaattaaa ccctttaatt gtttgccaga acacatcgct atggatgaat ttaaaagcgt
    18241 taaaaatgta actggatcaa tgagtttcat ttttatagat aatgatactc atgatgttat
    18301 agatatttta gaaaatagaa ctacaagatt cttgcgtgcc tatttcgagc gattcgattt
    18361 aaaaaatcga caacaagtta agacggttac tattgacatg tatgaaccct atgtccgatt
    18421 atttcgcgac ctatttccta atgcagctat tatttttgac agattccata tcgttcaaca
    18481 tttaaataga gaacttaata agtatcgtgt acaagttatg aatgaatacc gtaataaaaa
    18541 aggacctgat tatacaattt ttaagaataa ctggaaagtc ctattgatgg atactagtaa
    18601 aaccatattt agtaaataca gatggaataa atcttttaag gcttataaac gctcatctga
    18661 cattgtagaa ttcatgcttt caaaagacga tatactacga cactcctacg aacttgtcca
    18721 aggattacga aaagacctaa ggttatgtaa ttggcctaaa tttattaatc gtttaaattc
    18781 agttagtaaa aagtctg
//
LOCUS       DANRDI010000039        18178 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.039, whole genome shotgun sequence.
ACCESSION   DANRDI010000039 DANRDI010000000
VERSION     DANRDI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.039"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..291
                     /locus_tag="PZ377_001066"
     CDS             <1..291
                     /locus_tag="PZ377_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDM6357948.1"
                     /translation="TLQKTSLFGILKAQGFTNGYLANVVISQTVILALFGTAFGLLLT
                     GVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLFSILTIRKIDPLKAIG"
     gene            304..981
                     /locus_tag="PZ377_001067"
     CDS             304..981
                     /locus_tag="PZ377_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6357949.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            1189..2220
                     /locus_tag="PZ377_001068"
     CDS             1189..2220
                     /locus_tag="PZ377_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDM6357950.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            2563..3681
                     /locus_tag="PZ377_001069"
     CDS             2563..3681
                     /locus_tag="PZ377_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDM6357951.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            3656..4579
                     /locus_tag="PZ377_001070"
     CDS             3656..4579
                     /locus_tag="PZ377_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDM6357952.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            4590..5810
                     /locus_tag="PZ377_001071"
     CDS             4590..5810
                     /locus_tag="PZ377_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDM6357953.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(5915..7447)
                     /locus_tag="PZ377_001072"
     CDS             complement(5915..7447)
                     /locus_tag="PZ377_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDM6357954.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(7487..8368)
                     /locus_tag="PZ377_001073"
     CDS             complement(7487..8368)
                     /locus_tag="PZ377_001073"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDM6357955.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            8526..9386
                     /locus_tag="PZ377_001074"
     CDS             8526..9386
                     /locus_tag="PZ377_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDM6357956.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(9664..10464)
                     /locus_tag="PZ377_001075"
     CDS             complement(9664..10464)
                     /locus_tag="PZ377_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDM6357957.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            10604..11272
                     /locus_tag="PZ377_001076"
     CDS             10604..11272
                     /locus_tag="PZ377_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDM6357958.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(11399..12712)
                     /locus_tag="PZ377_001077"
     CDS             complement(11399..12712)
                     /locus_tag="PZ377_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDM6357959.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            13130..15202
                     /gene="lip2"
                     /locus_tag="PZ377_001078"
     CDS             13130..15202
                     /gene="lip2"
                     /locus_tag="PZ377_001078"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HDM6357960.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(15444..16271)
                     /locus_tag="PZ377_001079"
     CDS             complement(15444..16271)
                     /locus_tag="PZ377_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6357961.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(16344..17543)
                     /locus_tag="PZ377_001080"
     CDS             complement(16344..17543)
                     /locus_tag="PZ377_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDM6357962.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            17639..17731
                     /locus_tag="PZ377_001081"
     CDS             17639..17731
                     /locus_tag="PZ377_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357963.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            17845..>18178
                     /locus_tag="PZ377_001082"
     CDS             17845..>18178
                     /locus_tag="PZ377_001082"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDM6357964.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPG"
BASE COUNT         6200 a         2891 c         3500 g         5587 t
ORIGIN      
        1 acattacaaa agacgagttt atttggcata ttaaaagctc aaggatttac gaatggctat
       61 ttggcgaatg tggtaatttc gcagacggtc atattagcac tatttggtac ggcatttggc
      121 ttactgttaa caggcgttac aggtgcattt ttacctgatg cagtacctgt caaattcgat
      181 gtactaacat tgctcgtatt tgcaattgtg ttaatgattg tctctgtatt aggaagttta
      241 ttctccattt taacaattag aaaaatagat ccgttaaagg cgattgggta ggaggtgtag
      301 caaatgttga aatttgaaaa tgtaacaaag tcatttaaag atgggaatcg taacattgaa
      361 gcggttaaag atacaaattt tgagataaat aaaggtgata ttatagcatt ggttggacct
      421 tctggctctg gtaaaagtac atttctaact atggcaggtg ctttacaaac accgacatct
      481 gggcacattt taatcaataa ccaagatatt acgacaatga agcaaaaagc attggcaaaa
      541 gttagaatgt ctgaaatagg ttttatttta caagctacaa accttgtacc atttttaacg
      601 gtaaagcaac aatttacatt attgaaaaag aaaaataaga atgttatgtc taatgaagac
      661 tatcagcaac ttatgtcaca attaggtcta acttcattgc ttaataagtt accttcagaa
      721 atttcaggtg gtcagaaaca acgtgtggcg atagccaaag cgttatatac gaatccgtcg
      781 attattttag cggatgaacc taccgcggcg ttagatactg aaaatgcgat tgaagtcatt
      841 aaaattctac gtgatcaagc caaacaaaga aagaaagcat gtattattgt tacacatgat
      901 gaacgactta aagcatattg tgatcgttca tatcatatga aagatggcgt ccttaatctt
      961 gaaaatgaaa cagtagaata gttttattaa gccggtacat catgtgccgg tatttttatg
     1021 tttatgtatt atttgaataa actttcacat tcaattaata ataattatta tcgaaaatca
     1081 gaaatattcc gtgaaatata atattttttg tagtaaaatg gcctctaagt attcaatatt
     1141 taaatatggg gattgaatat aaaattatcg taatgggggt caatggttat ggatttattg
     1201 ataggtactt tatttttatt tttggtctta gtgattttta cattatttac atataaagcg
     1261 cctaatggta tgcgtgccat gggagcatta gctaatgcag caatcgcaac atttttagtg
     1321 gaagcattta ataaatatgt tggtggcgaa gtattcggta ttaaattttt agaagagcta
     1381 ggagacgctg cgggaggtct aggtggtgtc gctgccgctg gattaacagc attagctatc
     1441 ggtgtgtcac cagtatatgc attagttata gcagccgcgt gcggtggtat ggatttatta
     1501 ccaggtttct ttgcgggtta tatgattgga tatgtgatga aatatacaga gaaatatgtg
     1561 ccggatggtg tcgacttaat tggatcgatt gtcatcttag cgccattagc tcgtcttatt
     1621 gcagtattat taacgccagt agtgaatagt acattgattc gaattggtga tattatccaa
     1681 agtagtacga atacgaatcc aattatcatg ggtatcattt taggtggtat tattacggtt
     1741 gtcggcacag cgccattgag ttcaatggca ttgacagcat tattaggttt aacgggtgta
     1801 cctatggcta ttggtgccat ggcagcattt agttcggcat ttatgaatgg gacgctattc
     1861 catcgcttaa aattaggtga tcgtaagtct acgattgcag taagtattga acctttatca
     1921 caagcagata ttgtatcagc caatccaatt ccaatctata ttacaaattt ctttggtggt
     1981 gcgattgctg gtttaattat tgctatgtca ggtttaatta acgatgcgac aggtacagct
     2041 acaccgattg caggattttt agttatgttt ggatttaatc atccgacgac aattgtgatt
     2101 tatggtgtag taatggcgat tgtaggtgcg cttgcaggtt atcttggttc aattgtattt
     2161 aaaaaatatc caattgttac taagcaagac atgattaatc gaggtgcagt agacgcatag
     2221 catcatcata ttgaatagta aaaacaaata aaacatagta acgtgattca gtcgatgtaa
     2281 cagtcgataa tgagtcacgt ttttttatag aaaaatacaa gacataaaaa tgtcataatt
     2341 tattgtcgac aaatatcata ctgtataaac atttatcatt ttctcaagta ccttttacac
     2401 gatggaatga acttactttt tacgaaatta tgcgtatttt ataaacaaat atcattgata
     2461 taacggtaaa tgtaagcgtt tacaacagaa ataacagcat gctacgatat ttttgtaaat
     2521 tcactgattc aagtatttta agtcaatatg aggagggatg ttatgagcga ttctgagaaa
     2581 gaaattttaa aaagaattaa agataatccg tttatttcac aacgtgaact tgctgaggca
     2641 attggattat ctagacccag cgtagcaaac attatttcag gattaataca aaaggaatat
     2701 gttatgggaa aggcatatgt tttaaatgaa gattatccta ttgtttgtat tggcgcagcg
     2761 aatgtagatc gtaagtttta tgtgcataaa aatttagttg cagaaacatc aaatcctgta
     2821 acgtcaacac gctctattgg tggcgtagca agaaatattg ctgagaactt aggtaggctt
     2881 ggcgaaacgg tcgctttttt atctgctagt ggacaagata gtgaatggga aatgattaaa
     2941 cgattgtcca caccatttat gaatttggat catgttcaac aatttgaaaa tgcgagtaca
     3001 ggttcatata cagctttaat tagtaaagaa ggcgacatga catatggctt agcagatatg
     3061 gaagtgtttg actacattac gcctgaattt ttaattaagc gttcacactt attgaaaaag
     3121 gctaagtgca ttattgtaga tttgaattta ggcaaagagg cattaaactt cttatgtgcc
     3181 tataccacga aacatcaaat caaattagtt atcaccacgg tttcttcccc aaaaatgaaa
     3241 aatatgcctg attcattaca tgctattgat tggattatca cgaataaaga tgaaacagaa
     3301 acatacttaa atttaaaaat agaatctact gatgatttaa aaatagctgc taaacgctgg
     3361 aatgatttag gtgttaaaaa tgttattgtg acaaatggcg tgaaagaact catttatcga
     3421 agtggtgagg aagaaatcat taagtcagtt atgccatcaa atagtgtgaa agatgttaca
     3481 ggtgcaggcg attcattctg tgctgcagta gtgtatagct ggttaaatgg gatgtctact
     3541 gaagatatat taattgctgg tatggttaac gcaaagaaaa cgatagaaac gaaatataca
     3601 gttaggcaaa acctagatca acagcaactt tatcacgata tggaggatta taaaaatggc
     3661 aaatttacaa aagtatattg agtattctcg agaagttcag caagcacggg agaacaatca
     3721 accgattgta gcattagaat caacaattat ttcgcatggt atgccgtacc cacaaaatgt
     3781 tgaaatggca acaacagtag agcaaattat caggaataat ggtgccattc cagcaaccat
     3841 agccattata gatggcaaaa ttaaaattgg tttagaaagc gaagatttag aaatactggc
     3901 aactagtaaa gacgttgcta aagtatctag aagggattta gcagaagtta ttgcgatgaa
     3961 gtgtgttggt gctactactg tagcgacgac gatgatatgt gctgcaatgg ctggtattca
     4021 attttttgtt acaggaggta ttgggggcgt ccataaaggt gcagaacata cgatggacat
     4081 ttcagcagac ttagaagaac tgtctaaaac aaatgtcact gttatctgtg caggtgccaa
     4141 atcaatttta gacttaccta agacgatgga gtatttagaa acaaaaggcg ttccagttat
     4201 tggatatcaa acgaatgaat tgccagcatt cttcactcgc gaaagcggtg ttaagttaac
     4261 aagttcggtt gaaacgccag aacgacttgc tgacattcat ttaacaaaac agcagttaaa
     4321 tcttgaaggt ggcattgttg ttgctaatcc aattccatat gagcatgcct tatcaaaagc
     4381 atatattgag gcaatcataa atgaagctgt tgttgaagcg gaaaatcaag gtattaaagg
     4441 taaggacgcc acaccgttct tgttagggaa aattgtagaa aaaacgaatg gtaaaagttt
     4501 agcagcaaat ataaaacttg ttgaaaacaa tgcggcgttg ggtgctaaaa ttgctgtcgc
     4561 tgttaataaa ttattgtagg tgatgataca tgaatatttt attcgctatc acagggatag
     4621 catttgcact atttgttgcg tttttattca gttttgatcg taaaaaaata gacttcaaaa
     4681 agacgttaat aatgatattt attcaagtgt tgatcgtgtt atttatgatg aacacaacga
     4741 ttggtttgac aattttaact gcactaggtt cattttttga agggctaata aatattagta
     4801 aagcaggcat aaattttgtt tttggagata tacaaaataa aaatggcttt acgttctttt
     4861 taaacgtatt actgccatta gtttttattt ctgtattaat aggcatcttt aattatatta
     4921 aggtattacc atttattatc aaatatgtag gtatcgctat taataaaata actagaatgg
     4981 ggcgcttaga aagttatttt gctatttcaa cagcaatgtt tgggcaacca gaagtatatt
     5041 taacaataaa agatattatt ccaagattat ctagagcgaa attatataca attgcgacgt
     5101 ctggtatgag tgctgttagt atggcaatgc taggttcata tatgcagatg attgaaccca
     5161 agttcgtagt tacagcagta atgttaaata tttttagtgc gcttatcatc gccagtgtaa
     5221 tcaatcccta taaatctgat gatactgatg ttgaaattga taacttaacg aaatccacag
     5281 aaactaaaac attgaatgga aaaacaggaa aacctaagaa agttgccttt ttccaaatga
     5341 ttggtgatag tgcgatggat gggtttaaaa tcgctgttgt agtagccgta atgttgttag
     5401 catttatttc attaatggaa gcaattaata tcatgtttgg tagtgttggt ttgaacttta
     5461 aacagcttat tggctatgtg tttgcaccaa tcgcattctt aatggggatt ccatggagcg
     5521 aagctgttcc agctggctct ttaatggcga ctaaattaat tacaaatgag tttgtagcaa
     5581 tgcttgattt taaaaatgtc ctgggtgatg tatcagctcg aacacaaggt atcatttcag
     5641 tttacttagt aagcttcgct aattttggta cggttggtat catcgtaggt tcaattaaag
     5701 gcattagtga taaacaagga gaaaaagttg catcctttgc aatgaggttg ctacttggtt
     5761 caactctagc ttcaatcatt tcaggatcaa tcattggctt agtattgtaa atgaatcgaa
     5821 gtacctaaat taaattcatg gcaaagctaa accccgtcac caagttggcg caacagcgca
     5881 tcataactta gtgacggggt tttatcataa caatctactt tttcgtagcc gtttttgaaa
     5941 tgtatgttga tggtttatct ttttcaaaaa ttgttaatcc cgttatatct tttttatgtt
     6001 ttgaagggac aatgaagcta agtatataag caaagacaaa agcaactgta aatgaaatgg
     6061 tagatacata gaaaggtgag ttacctttgc caacaccatt atagacataa gcaaagatga
     6121 tacccaatat taatccacaa ataacaccga atgtattcgt acgtttagtg aaaataccaa
     6181 ctgcaaatac accagccaat ggaacgccga ataatccagt cacaaacaag aataaatccc
     6241 ataagtcatt tgaattagaa gcaattaagt atagtgacat tccaaaaccg aaaatacctg
     6301 caatgataat aatgaaacgt gcaaagttaa cttcgtgtcg ctcgctacct tttccgaaga
     6361 agcgttgctt aatgtcgatt gaaatacaag cagatataga atttaaacta gatgaaatgg
     6421 tagactgtgc agcggcgaaa atggctgcaa taagtaatcc tgctacaaat ggtggcatct
     6481 cagtcaaaat gaaatatggc actacagatg atgtattgaa gccttttggt aaaacagctt
     6541 catgtgtata aaatgaatac agcattgtac ccataccata aaataagggt gctgaaatta
     6601 aagctaggat accatttgtc cataacgatt tatttgtttc ttttaaacta tcagaagctt
     6661 gataacgctg cacgacgtct tgactcgctg tgtattgata caagttgttg aaaatatttc
     6721 ctaggaaaat aattggaatg gcagctgccg cagtatttag tttccaattg tctgcactaa
     6781 ttaatttttt gtgctcaatc gcatctgcaa agacagtgcc gaaaccgcct ttaatgttca
     6841 caacacctag aataataata actaaagcgc cgcctaataa aatgacgcct tgaatgaaat
     6901 cactccaaac cacaccttcg aaaccaccta aaaatgtata taaaatacat agtaaaccaa
     6961 cgagtgatgc aacgatataa gggttcatgt ctgatacaga tgtgattgct aatgttggta
     7021 agtagataac aattgcaaca cgccctaaat ggtaaacgac aaataataat gagccaatga
     7081 cacgtatgct agggccaaat ctagcttcta aatattcata tgcagatgtt acctttaact
     7141 ttttaaagaa agggacatag aaataaataa gtaatggaat aattgcgacg atagcaatgt
     7201 taccagcgat atatgaccaa tctgttaaaa atgctttctc tggtgtcgac ataaatgtaa
     7261 tcgcacttaa cgtagtagca taaattgaaa agccaactac ccaagatggc aagcgaccac
     7321 ttgcggtaaa gaaactattg gtactttggc tcgcgcgctt ggtaaaataa acgccaatga
     7381 acaacatagc tagtagataa atgataacgg caacccagtt tagtgtgcca aatccaactt
     7441 ctttcatggg caacatcccc tttacaatgt attgattctt tgatgtctat aaatcgtatt
     7501 ttgcaatgag ttgatctaat gtttgtcgat gtgcttcgtt aaaaggtttg aaaggtcttt
     7561 tcggtaatcc tgcatcaatg ccacgatgac gtaatatttc tttcaatgtt ggataaatcc
     7621 ccattgataa cactgtttcg ataatgtcgt ttgaatcatg ttgcagttgg taagcttctt
     7681 gaatttgacc ttgtcgtgct aagtcgaaga tttttcttgc acggcgacca ttaacgttat
     7741 atgtagaacc aattgcacca tctacgccag aaatcgtagc ttgaactaac atttcatcaa
     7801 agccagataa gattaatttg tctgggaatg cttttctaat acgttcgagt aggaagaagt
     7861 ttggcgctgt atatttaaca ccaacaattt tttcatgatt aaatagctcg ctgaattgtt
     7921 caatagaaat attcacacct gttaaatctg gtattgcata aataatcata ttgttctgag
     7981 ttgcttcgat aatatcgaaa tagtaatctc taatttcttc aaaagtaaat ggatagtaga
     8041 atggtgttac ggcagaaagt gcatcataac cgagttctgt ggcatatttt ccaagttcaa
     8101 tggcttcatt taaatctaac gaacctactt gagcaatcaa tttcacttta tccccaactg
     8161 cctctttggc aaccttgaaa acttgcttct tctgctctgt atttaataaa aagttttcgc
     8221 ctgagctacc atttacataa agaccgtcta attcttcagt ttcaatggca ttttgagcaa
     8281 tttgtttaag tccttgttca tttacttgac cattttcatc aaaaggaacg agtaacgctg
     8341 catataaacc ttttaaatct ttgttcatta tgaagtccct ccaaaaatca tttgataata
     8401 tagtttacag ctataattgt aaacgctatc ataaaatgta acaatatctt tttgaaaatt
     8461 gtagtcatat ttatgtataa ttaatgaaaa tgtttttcaa aatcaataga aatggagtga
     8521 gtaaggtgta ttacatcgca atcgatattg gaggcactca aattaaatcg gcagttattg
     8581 ataagcaatt gaatatgttt gactatcaac aaatatcaac gccggacaac aaaagtgagc
     8641 ttattactga caaagtatat gagattgtaa caggatatat gaagcaatat cagttgatcc
     8701 aacctgtcat aggtatttca tcagcaggcg ttgttgatga acaaaaaggc gaaattgtat
     8761 acgcagggcc aaccattccg aattataaag gtactaattt taagcgatta ttaaaatcac
     8821 tgtctcctta tgtcaaagta aaaaatgatg taaacgctgc attactaggc gaattgaaat
     8881 tacatcaata tcaagcagaa cggatctttt gtatgacgct tggtacaggc attgggggtg
     8941 cgtacaagaa taatcaaggt catattgata atggtgagct tcataaggca aatgaagttg
     9001 ggtatttatt gtatcgtcca actgaaaata caacgtttga gcaacgtgct gcaacgagtg
     9061 cattgaaaaa gcgcatgatt gccggaggat ttacgagaag cacacatgtg ccagtattgt
     9121 ttgaagcagc tgaagaaggt gatgatattg caaaacaaat attgaatgag tgggcagaag
     9181 atgtagcaga agggattgcc caaatacagg tcatgtatga tccagggctt atattaattg
     9241 ggggcggtat atctgaacaa ggagataatc tcattaaata tatcgagccg aaagttgcac
     9301 actatttacc aaaagactat gtttatgcac caatacaaac gactaagagt aaaaatgatg
     9361 cagcattata tggctgtttg caatgatagt tgaaagaagg agtcattcta aaatagaatt
     9421 tgaaaccgtt acgagagatg agagctgttg ttagttccac acatcacact ctatctagga
     9481 ccaatctaaa ctatatcaac caacagtgtg ccacgggcaa attaaattga agaagctgag
     9541 atattaaaat tttagaaaat gtaaaaaaat atttggtatt gaaattaaaa aagcacctag
     9601 caactcgttg ggacaatcac gatgattgtc tacagttgca ggtggatttg aatatactac
     9661 tagttatttg ttgtctagga taatagattt agtatgttga taagtttgac tcagattcgt
     9721 attttctaat aaatgataac tcacgatatc gattaaaaag agtgtcgcaa tttgtgtgtt
     9781 gataaattga tggtcggtat tacgcgattg atccgttgtt aaaagtacta aatctgcaca
     9841 atctgtaagt ttactacctt caaaatttgt gatggcaacg acatatgcac catgagattt
     9901 ggcgacttcc gctgcagaaa ttaattccga agtattacca ctatttgaca tagcaataaa
     9961 catatccgaa tgagatagta gggatgccga tattttcatt aaatgtgaat cggtagtaac
    10021 attacctttt agccccatac gaatcatacg ataataaaat tcagtcgctg ataaaccaga
    10081 gctacctagt ccagcaaaga gtatatgtcg acttgattga agtttgtcga taaaggtttg
    10141 gataatgtcg ttatcaataa attcaccagt ttgttgaatg atttgttgat gatatttatg
    10201 aattctttga ataattgggc tattttcaat aactgtctct gtcatttctt gttgaatatt
    10261 aaattttaaa tcttggaaat tctcataatc cagcttatga ctaaagcgtg tcatcgttgc
    10321 tggtgatgta ccaatcgcat gggctaagga gttaatcgtt gaaaaggcat cgctataacc
    10381 attttgtctt atataattga cgatgcgttt atcagttttt gtaaataaat gttgataacg
    10441 ttgaacacga ttctcaaatt tcattgtgtc accccttcat cttaatgatt actattatat
    10501 atgaaaaata ttttcaagat agtaaaaagc attgataaaa attatcttaa tgatatattg
    10561 taaatgactt tacgtgaaaa aacgacttat ggagtgagga ataatgttac cacatggatt
    10621 aatagtatct tgtcaggcac taccagatga accattgcat tcatctttta ttatgtcgaa
    10681 aatggcatta gctgcgtatg aaggtggtgc tgttggtatt cgcgcaaata ctaaggaaga
    10741 cattttagca attaaagaaa cggtagattt accagttatt ggcattgtga aacgtgacta
    10801 tgatcactca gatgttttca ttactgcaac gtcaaaagaa gttgatgaac tgatagaaag
    10861 ccaatgtgaa gtcattgcat tggatgcaac gttacagcaa cgtccgaaag aaacgttaga
    10921 cgaattagta tcatatatta gaacacatgc accgaacgtt gaaatcatgg ctgatatcgc
    10981 gaccgttgaa gaagctaaaa atgccgcacg acttggcttt gattatattg gcacgacgtt
    11041 acatggctat actagttata cgcaaggaca attactttat caaaatgact tccaattttt
    11101 aaaagatgta ctacaaagtg ttgatgcaaa agttattgcg gaaggtaatg tcattacacc
    11161 ggatatgtat aaacgtgtga tggacttagg cgttcattgt tcagtcgttg gtggtgcgat
    11221 aacacgacca aaagaaatta cgaaacgttt tgttcaaatt atggaagatt aaatgataac
    11281 gataaaaaaa cgagatgacc atcattaatt aaaggcacct aattatctta ggtggctgaa
    11341 tgaatgtaat gggttcatct cgttttgttt gtttatgata gtgattttat tttcaacttt
    11401 atccaaaaat aagtaaagcg acggggatgg tgattaatag cgacaacgcc acgcgtaaaa
    11461 accaaatgat gatgagtttc cagacaggta ttttaatttc agttgctagt atacatggca
    11521 ctaatgctga gaaaaagata atggctgata cgcttactac accgacgaca aatttagtac
    11581 tcattgcagc tttagttact aacaaagatg gtagaaacat ctctacaata gaaatcgctg
    11641 acgcttttgc tagtaaagcc tgatcagcaa ttgggaaaat ataaataaat ggatagaaga
    11701 tatagccaag ccaatcaatg aatggtgtat agttcgctac aatcagtcct aaaaaaccaa
    11761 tcgataatat agaaggtaaa ataccaacag tcatttctaa accgtctttc aaattgtccc
    11821 aaacgttctt cacgagagat ggtgttaatg cattttgttt catcgcctct gcatatgcag
    11881 ttttcagtct gcttccttca atagcaactt cttgttctcc ttcttgtccg ttataatatt
    11941 ctgttgattc attgctgatt ggcggtagcc atgcagtaat tgcagtcacg acaaatgtga
    12001 tgactaaagt tatccaaaag tataaattcc aatgcggcat taatcctaaa gttttagcaa
    12061 cgataatcat aaaagttgct gaaactgttg aaaagccagt cgcaataatc gtggcttctc
    12121 gtttgttgta catcccttgc ttatagacac gattagtaat caataatcct aaggaataac
    12181 tgccgacaaa cgaagccact gcatcgacag cggattttcc tggtgtttta aaaataggtc
    12241 tcataatagg ctccatataa acaccgacaa attctaataa gccatagccc actaataaag
    12301 aaagcgcaat tgcacctact ggaattaaga tacttaatgg catcattaat ttttcaaaca
    12361 aaaacggacc atagttagct ttaaatagta ttgatggacc gattttaaat acatacatta
    12421 taccgatcat tgcacctgca actttaaata atgtaatgac caagtttgtg attgaagtca
    12481 taaaagtacg tctcactatt ggtaacgctg taccaattaa aatcataatc agtgcaacat
    12541 agggcataag tggacctatg attgagcgaa tggctagatg aacatgatcg acgaaaatag
    12601 tgttgttacc attaatcgta aaaggaataa agaaacatag tatgcccact aaactataga
    12661 caaaaaaacg ccatgcactt ggttgttgtg cattagaatg atattgattc attaaagcaa
    12721 cccctttgtt taaatgaata cacaaaactg tatgatgcat cttcccctta atgagatgaa
    12781 tcattatttt aatttagaaa aatctgaaaa cttactataa ttgtatagtt tgaattattt
    12841 tcataccaat acaaattaac taattatata tagattgaaa ctatattact taataaaata
    12901 tttatcttaa atgttgttgt gttgattcaa caccacaact aaaagtgttt ataaattatt
    12961 tggaaataca catatttgta aatgattagt atcgatttaa tatcgtatta ttaaattttt
    13021 attaattttg tagtcttaat caaaaaataa tatatgtcat gttatattga aggtgcagtt
    13081 gtttttcatt ctcaagaggg ggtcaaaaaa atacttttga ggtgattata tgttaagagg
    13141 acaagaagaa agaaagtata gtattagaaa gtattcaata ggcgtggtgt cagtgttagc
    13201 ggctacaatg tttgttgtgt catcacatga agcacaagcc tcggaaaaaa catcaactaa
    13261 tgcagcggca caaaaagaaa cactaaatca accgggagaa caagggaatg cgataacgtc
    13321 acatcaaatg cagtcaggaa agcaattaga cgatatgcat aaagagaatg gtaaaagtgg
    13381 aacagtgaca gaaggtaaag atacgcttca atcatcgaag catcaatcaa cacaaaatag
    13441 taaaacaatc agaatgcaaa atgataatca agtaaagcaa gattctgaac gacaaggttc
    13501 taaacagtca caccaaaata atgcgactaa taatactgaa cgtcaaaatg atcaggttca
    13561 aaatacccat catgctgaac gtaatggatc acaatcgaca acgtcacaat cgaatgatgt
    13621 tgataaatca caaccatcca ttccggcaca aaaggtaata cccaatcatg ataaagcagc
    13681 accaacttca actacacccc cgtctaatga taaaactgca cctaaatcaa caaaagcaca
    13741 agatgcaacc acggacaaac atccaaatca acaagataca catcaacctg cgcatcaaat
    13801 catagatgca aagcaagatg atactgttcg ccaaagtgaa cagaaaccac aagttggcga
    13861 tttaagtaaa catatcgatg gtcaaaattc cccagagaaa ccgacagata aaaatactga
    13921 taataaacaa ctaatcaaag atgcgcttca agcgcctaaa acacgttcga ctacaaatgc
    13981 agcagcagat gctaaaaagg ttcgaccact taaagcgaat caagtacaac cacttaacaa
    14041 atatccagtt gtttttgtac atggattttt aggattagta ggcgataatg cacctgcttt
    14101 atatccaaat tattggggtg gaaataaatt taaagttatc gaagaattga gaaagcaagg
    14161 ctataatgta catcaagcaa gtgtaagtgc atttggtagt aactatgatc gcgctgtaga
    14221 actttattat tacattaaag gtggtcgcgt agattatggc gcagcacatg cagctaaata
    14281 cggacatgag cgctatggta agacttataa aggaatcatg cctaattggg aacctggtaa
    14341 aaaggtacat cttgtagggc atagtatggg tggtcaaaca attcgtttaa tggaagagtt
    14401 tttaagaaat ggtaacaaag aagaaattgc ctatcataaa gcgcatggtg gagaaatatc
    14461 accattattc actggtggtc ataacaatat ggttgcatca atcacaacat tagcaacacc
    14521 acataatggt tcacaagcag ctgataagtt tggaaataca gaagctgtta gaaaaatcat
    14581 gttcgcttta aatcgattta tgggtaacaa gtattcgaat atcgatttag gattaacgca
    14641 atggggcttt aaacaattac caaatgagag ttacattgac tatataaaac gcgttagtaa
    14701 aagcaaaatt tggacatcag acgacaatgc tgcctatgat ttaacgttag atggctctgc
    14761 aaaattgaac aacatgacaa gtatgaatcc taatattacg tatacgactt atacaggtgt
    14821 atcatctcat actggtccat taggttatga aaatcctgat ttaggtacat ttttcttaat
    14881 ggctacaacg agtagaatta ttggtcatga tgcaagagaa gaatggcgta aaaatgatgg
    14941 tgtcgtacca gtgatttcgt cattacatcc gtccaatcaa ccatttgtta atgttacgaa
    15001 tgatgaacct gccacacgca gaggtatctg gcaagttaaa ccaatcatac aaggatggga
    15061 tcatgtcgat tttatcggtg tggacttcct ggatttcaaa cgtaaaggtg cagaacttgc
    15121 caacttctat acaggtatta taaatgactt gttgcgtgtt gaagcgactg aaagtaaagg
    15181 aacacaattg aaagcaagtt aaattcatct tctgaattta atatgctatg taaatcgtgc
    15241 tgttatcacg gcacatcaga tataagtagc atcacagtgt tgaatttaaa aatagtaaag
    15301 tgaaataaag cgcctgtctc attagcgaaa actaaaggga caggcgtatc tgtttatgag
    15361 cttaataaat tgtatgaata atatggttga tcgaataact gtttatcatg atgataaatt
    15421 gagtttttta aaataatgat atattacatc attgttatag cgtttaagaa atcaacaact
    15481 ttacgataaa tagtgattgc ttcgtcatta ggtctacgat caaaatcatg ctcgttttta
    15541 ttcacgcgtt caaatgttga atgtggaaca tgattcatga tatgttcgct ttcctcaacg
    15601 ggaacatcat aatcgccatt acaatgcgca atgaaaacag gtggaagtgt tttaagttca
    15661 tctggtgcaa tattatattt tgaattagta taatcagcaa tgttaatcat atttatccat
    15721 ttacctgtgc cacgtgcata aacgtagatt aaaaaacgtt gtgcgatttg atcttgaaca
    15781 accggtgttg gtgaagtgag ttgtgcaatc attgtttcgt ttacgctttg agctattttt
    15841 gcgtaataac tattagttgt tttaaaaggt tcagtgttga tgcgactata accataaaaa
    15901 tcaataacac catcaatatc tctgtctcgt gcaattaata gacttaaata tgcacctgat
    15961 gatctgccaa aggtaaaaat agggcaatta gaatattgtg attgaatcgc atcgaatgat
    16021 gcgtagacat cctcaataat gcaatcgaga cttacttctg gtaataaacg ataacttagt
    16081 tgaattaaat cgtaatgttc cgtaaggata tcgatatact gtggggataa atcgttagct
    16141 ttaccgaaca ttaatccacc accgtggatg tagacaatag cgccttttgt tggttgattt
    16201 tttgctttaa taattgtgta aggtaatgca aatgcatctt tagtaattac tttatcttta
    16261 atttcagtca cgatttaata ggctccttat ttttgatatt gatgtcatta taacactgtc
    16321 ttaaatttcc atgaaaaata gtcttaagac gatgagtcat gataattctg ttccaattga
    16381 cgtaaagcgt cacgggtatg cttctttaga ccttccccat aatccatcat tttaacaata
    16441 tctttaaaag cagcatgtgg aatggctaaa tcttctaaat ctgccataga aaattcaaga
    16501 ttgatatcat gtggtcgctg ttcagcaagt ttatgcacaa agtcaggttc tgtgacaaaa
    16561 ggcgaagaca tgccgaccat atctgcatgt tgtaaagcat ctaaagcaga ctctggagaa
    16621 ttaatcccgc cacttgcaat taaagggata cgacctgcta aatgttcata gacaatttgg
    16681 ttaactggtc gaccgaaatg atcacctggt gtacgagacg tattttgata aatatgtcga
    16741 ccccagctag cgattgctaa gtattggatg tttgaaacgt ccatgaccca attgattaat
    16801 tggttgaact cgtcaatggt atatcctaaa tcactgcctc tggtttcttc tggcgttgct
    16861 cgaaatccta aaataaaatt gtcaggtgct tctttatcaa tcacttcttg taccgcacgc
    16921 ataacttcta aacataatct tgcacgattt tttaatgagt cggcaccgta atggtctgta
    16981 cgtttattcg aaaaagttga gaaaaatgtt tgaatcagca aacgttgtgc aatcgaaatt
    17041 tccacaccat caaaacctgc tttaatcgcg cgtaatgtag catcgcgata ctgctgaatg
    17101 atgctattga ttttctcatg agacatggcg ataacatcgt gttcaatcgg tgaatgcaat
    17161 gtcatagggc ttggtccata cacctttcca aaatttaaaa tggcttgatt tgaaaaacga
    17221 ccagcatgcg ctagctggat aatagcgagg ctaccatgtt gtttcatcgt agatgccatg
    17281 ttagttaatc cagggataca agcatcatga tcaatattaa agccatattc aaacaattga
    17341 ccataaggtt caatgtaagc agcgccggtg acttgcattc cagctgaatt agagcgacgt
    17401 gcagcataag ccaagtcttc ttttgtaata tagccttctt ttgttgatgt gtttacggtc
    17461 attggtgata atacaaagcg attcgaaatt ttgatgccat taggtaagtg gattgattgt
    17521 aaaagtggtt tgtatcggta catactatga ttccttttct attcaatatt gttttcaaag
    17581 taccatggaa agaatgaata atcaatgatg aacagtcttg atagaataga attggtacat
    17641 ggaaagtatt tttaaaatta aactaatgaa tggcatttgt aggtctgaaa atatgaatat
    17701 gaaaaagaaa aataaaggcg aaaagatata aaagttaatt gaaaaacgtt atcatatacg
    17761 tgggtatatg aagagggaat ggtattaaga acgctaaaat gttatgtcgg tttgacatga
    17821 caggataagt ttggagatga cggattggtt aaattaagcg tattagacta tgccttaata
    17881 gatgaaggta aggatgcaca aaaggcattg caagattcag tgacacttgc aaaattagca
    17941 gatcgacttg gctttaagcg aatttggttt acggaacatc ataatgtacc agcgtttgcg
    18001 tgtagtagtc cagaactttt gatgatgcat acattggcgc agacaaatca catacgagtt
    18061 ggctctggtg gtgtgatgct gccgcactat cgaccttata aaattgctga gcattttaga
    18121 atgatggcag cgttatatcc aaatcgtatt gatttaggta ttggtaataa tccaggta
//
LOCUS       DANRDI010000040        18121 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.040, whole genome shotgun sequence.
ACCESSION   DANRDI010000040 DANRDI010000000
VERSION     DANRDI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.040"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..274)
                     /locus_tag="PZ377_001083"
     CDS             complement(<1..274)
                     /locus_tag="PZ377_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H) small subunit"
                     /protein_id="HDM6357965.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVY"
     gene            complement(278..2683)
                     /locus_tag="PZ377_001084"
     CDS             complement(278..2683)
                     /locus_tag="PZ377_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDM6357966.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(2749..3480)
                     /locus_tag="PZ377_001085"
     CDS             complement(2749..3480)
                     /locus_tag="PZ377_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDM6357967.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(3710..4246)
                     /locus_tag="PZ377_001086"
     CDS             complement(3710..4246)
                     /locus_tag="PZ377_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6357968.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(4479..5303)
                     /locus_tag="PZ377_001087"
     CDS             complement(4479..5303)
                     /locus_tag="PZ377_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDM6357969.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(5520..5702)
                     /locus_tag="PZ377_001088"
     CDS             complement(5520..5702)
                     /locus_tag="PZ377_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357970.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(5786..6253)
                     /locus_tag="PZ377_001089"
     CDS             complement(5786..6253)
                     /locus_tag="PZ377_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDM6357971.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(6439..7989)
                     /gene="adcA"
                     /locus_tag="PZ377_001090"
     CDS             complement(6439..7989)
                     /gene="adcA"
                     /locus_tag="PZ377_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDM6357972.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(8178..8249)
                     /locus_tag="PZ377_001091"
                     /pseudo
     CDS             complement(8178..8249)
                     /locus_tag="PZ377_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8284..8550)
                     /locus_tag="PZ377_001092"
     CDS             complement(8284..8550)
                     /locus_tag="PZ377_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDM6357973.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(8550..8801)
                     /locus_tag="PZ377_001093"
     CDS             complement(8550..8801)
                     /locus_tag="PZ377_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDM6357974.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(8966..9067)
                     /locus_tag="PZ377_001094"
     CDS             complement(8966..9067)
                     /locus_tag="PZ377_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357975.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(9074..9673)
                     /locus_tag="PZ377_001095"
     CDS             complement(9074..9673)
                     /locus_tag="PZ377_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDM6357976.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(9692..10051)
                     /locus_tag="PZ377_001096"
     CDS             complement(9692..10051)
                     /locus_tag="PZ377_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDM6357977.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            10306..11556
                     /gene="fmhA"
                     /locus_tag="PZ377_001097"
     CDS             10306..11556
                     /gene="fmhA"
                     /locus_tag="PZ377_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDM6357978.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(11653..12384)
                     /locus_tag="PZ377_001098"
     CDS             complement(11653..12384)
                     /locus_tag="PZ377_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDM6357979.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(12381..13100)
                     /locus_tag="PZ377_001099"
     CDS             complement(12381..13100)
                     /locus_tag="PZ377_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDM6357980.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(13081..13860)
                     /locus_tag="PZ377_001100"
     CDS             complement(13081..13860)
                     /locus_tag="PZ377_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDM6357981.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(13981..15420)
                     /gene="mdeA"
                     /locus_tag="PZ377_001101"
     CDS             complement(13981..15420)
                     /gene="mdeA"
                     /locus_tag="PZ377_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDM6357982.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            15594..15698
                     /locus_tag="PZ377_001102"
     CDS             15594..15698
                     /locus_tag="PZ377_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDM6357983.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(15852..16538)
                     /locus_tag="PZ377_001103"
     CDS             complement(15852..16538)
                     /locus_tag="PZ377_001103"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDM6357984.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(16865..17731)
                     /locus_tag="PZ377_001104"
     CDS             complement(16865..17731)
                     /locus_tag="PZ377_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDM6357985.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
BASE COUNT         5770 a         3460 c         2483 g         6408 t
ORIGIN      
        1 cataaacatc tacacaacct tcatcaggtt cttgaacaat acctgtattt aaatcaattt
       61 tttgatcgtg gagcgggcaa aatacatatt ccccactcac tgtcccttca gacaatggtc
      121 cttgtttgtg tggacagata ttgtgaatcg catgaatttt gccactttct gttaaaaaca
      181 accctacctc tttgcctttg acaataacct tttttccaat taggggtgtt aattcatcta
      241 tagttgtcac tttaattttt tcttttgttt ccatgtatta caccttctcc acttcaaaaa
      301 ttctacgtgc ttgcgcattg ctagttattg cttcccaagg ttcagcttcg actgcttttt
      361 tagcatccat aatgcgttca aatagttcat tttgtctttc tgggtcaagt aagacttctt
      421 ttacattttc aaatccaagt cttcttaacc atggcgctgt tctttcagca tatatacctg
      481 tttcgcgata atattgcatc aaagcaccac ataatttgat tacttcatct tctgtttcta
      541 cagttgttaa aaattcagct ttttcaactt ctgtaccacc attaccaccg atatagattt
      601 ggaatccatt ttcaactgag ataataccaa aatctttaac acctgattca acacaacttc
      661 ttgggcagcc tgatacaccc attttgaatt tatgaggtgt atcgatgtat tcaaatgttt
      721 tttctaaacg aatgccaagt cgtgtcgtgt attgcgtacc aaatcgacaa aactctttac
      781 caacacagct tttaactgag cgtgttttct taccataagc tgatgctgaa cgcataccta
      841 ggtcttccca tatatttggt aattcttctt ttttaactcc atacaaacca acacgttgtg
      901 aacctgtcac tttaactagt ggcacatgat atttcttagc cacttctcct agacgaatca
      961 gttggtctgc atctgtaaca cccccacgca tttgaggtat aacagaaaat gtaccatcat
     1021 tttgaatatt cgcatggtaa cgttcgttag caaatcttga ttctctttca tcttcatgat
     1081 catgtggata aaccatgttt aaataatagt tgattgctgg tcgacatttt ggacatccac
     1141 ctttattttt aaagtttaaa acatgtcgaa cttctttaga tgtttttaaa cctttcgctc
     1201 ttatttgcgt tactatttga tcgcgtgtca aatcagtaca accacatata ccagcaggtt
     1261 ttgcggcaac aaagtcatct cctaaggtgt gctgcaatat ttgagcaatt tgcggtttac
     1321 atttaccaca tgaattcccc gcttttgttt tagccgttac ttcttcaact gttgtaaagc
     1381 cattttccgt aatcgcattt actatagtac ctttatcaac accattacaa ccacaaattg
     1441 tttcatcatc agccatatca gcaattgata gcgatgcctc ttctccacct ttagtaagca
     1501 atgatacaag tgtgtaatct tcagtggatt cacctttttt catcatgtta taaaagcgtg
     1561 aaccatcatc gatatcacca tatagtactg caccaactac attaccgtct tttaaaaaga
     1621 tttttttata gttattatca acactattaa atatttcaat acctttaatt tctgcatttt
     1681 ctacaatttg accagcacta tacaagtcac acccagaaac ttttaatgac gtaaatgttg
     1741 ttgatccctt gtatccgttc gtttctttat ttgttaaatg atcagctaat actttacctt
     1801 gttcatatag tggtgcaacg agtccataaa ctttgccgtt atgttctgca cattcaccaa
     1861 ctgcatatac attgctatca cttgtttgca tcacatcatt gacaacaata ccacgattaa
     1921 catctagacc tgattctttg gctacttctg tgtatggtcg tatacctact gccataacaa
     1981 ctaagtctgc cggaatctcg cgtccatcag ccaatttaac accctcaaca tcatcttctc
     2041 ctaagatttc agttgtgttg gcttgcattt caaacttcat accttgcttt tctagatctg
     2101 ctttaagcat atttccagct ttacggtcta gttgcatttc catcaaccat tcagctaaat
     2161 gtaacaccgt tacttccata ccttgatcta ataaaccacg tgcacactct aaacctagta
     2221 atcctccacc aattacaatt gctttctttt tagtcttagc aatgttcatc atttgttcag
     2281 tatcatcaag cgttctccaa ccaattacac tcggtaatgt cgaacctgga actggaatga
     2341 cgaatgcttt agaacctgtc gcaaaaatca atttatcgta tgatacttca ataccatttg
     2401 cagtagtaac tgattgattt gctctatcta cttcaattac aggatcattt gtaattaact
     2461 cgataccatg ttcctcatac cactcatatg gattcataat tgtttcttca actgtcattt
     2521 tattttgtaa aatatttgaa agcatgatgc ggttatagtt tggataaggt tctttaccta
     2581 ttaccgtaat atcatataaa tcgttggcgc gctctaatat ttcttcgatt gttcgaatgc
     2641 ccgccatacc gttaccaatc attactagtt tttgctttgc cataaaatat gcccctttac
     2701 tccataatat ttatttcaaa aaaaggtatt aatttttcgt tagtgctttt atattttcat
     2761 tggaatcatt aagctttcta atctatcgtt aatgatttgc tttaaaattg ggtcgaagtt
     2821 aattgaaggt gtgaagtgta tatctgtatt aataaccatg tcattcattt gctgcttcac
     2881 tttgttaaca agtcttccgt catataaaaa taatggtacg acaatcaatt tttgataccg
     2941 tttcgagatg ctttctaaat catgtgtaaa actaatctct ccatatagcg ttctcgcata
     3001 tgtcggcttg ctaatttgca aattttgagc gcatatttgt aactcttcgt gtgccttagt
     3061 aaactttcca ttaatattgc cgtgtgcaac aaccataact ccaacttgtt gttcgtcacc
     3121 tgctaatgcg tcacaaatac gttgttcaat taatcgtctc attaaaggat gtgtgccaag
     3181 tggctcgctt acttctacct ttatgtctgg ataccgtcgt ttcatttcat gaacgatatt
     3241 cggtatatcc ttgagataat gcattgcact aaagattagc aatggtacaa ttttaaaatg
     3301 gtcaacccca ctttgaatca acgtcgtcat taccgtctct aaatcctgat gctcactttc
     3361 taaaaacgca atatcatagt gatgtatatc atcttttact aattcagaaa taaatgcttc
     3421 taacgcttga ttctgtcgtc cgtgcctcat gccatgtgca acaatgatat tcccattcac
     3481 atttaccaac cctttcacac gtattgtata ccaaatcatt ttgtttttgt gaaaagaatc
     3541 acattataat gtaaaatcag ggaattccct gatgcctgta gtcatgcata ttccttatac
     3601 attttccctt tttgttaaat caaaaaaagc gaccgatata tgaatcccta ctcaacattt
     3661 atttgagcaa gcattaatat atcggtcgct tgtagtgtat attattatct taaaatggtg
     3721 gttggcctaa tattgtttcg tcaaagcgct cgggtatcaa tactttgcgc atgatcacac
     3781 ctaaatcgcc atcatcattt tcatgttcgc tgtatatttc ataacctctt ttttcataaa
     3841 ttttaagtaa ccacggatgc aatcttgcag atgtacctaa agtaactgcc gctgacttta
     3901 acgtatctcg caaaaatgct tcttcaacat aagtaagtaa ttggctacca tagcctttcc
     3961 cttcatactc aggatttgtc gcaaaccacc agacaaaagg ataacccgaa atacttttca
     4021 cacttcccca aggatatcta accgtaatcg tagatataat ttcatcatca attgtcatga
     4081 caaatgtagt atttttatct atattttctt taacagcatc taaattagca ttaaccgaag
     4141 gccaatcaat acctagttct cttagaggcg taaatgcttc atgcatgagt tgttgcaatt
     4201 tttctgcatc ttgttcactt gcgagtcgaa tcatcgtttt tgtcatatta atccccactc
     4261 ttttttaaat gatttaacca tattttattt ttaaaataaa tatccatcaa agtgtatcaa
     4321 taaatttatc acatgtcaga aagtatgctt catctgaata caccaatact ctcatgaaac
     4381 ttattaaaaa ttactctctc aacgtaaaaa accattcaaa ttcatgaatg gtttggaaga
     4441 atgattcatt gttacgctat ttaatcacta catcttaatt attgttgctc taaacgatta
     4501 cgcttaccat ttaagaaagc ataaacgaga cctacaaaaa taccgccacc gacaaagtta
     4561 cctaagaaag caaaaacgat attttttaaa acgtgtaacc atgaaactgc atcaaggtta
     4621 aagaatacca tacctgcata tagacctgca ttgaacacaa cgtgctcata tcccatgtat
     4681 acaaagacca cgacaccaca agctatgaag aatgcctttg ttaagccgcc tttgaattgc
     4741 atagagatga aaataccaat attaataaag aagttacaga aaataccttt tgtaaaaata
     4801 ttcaaccatg ttgaatcaac agtctttttc tgaactaaag ctgttaaagc ttgtgtcatt
     4861 tcaggtgtca tgacatgcgc aaatttcatt aagaaaaata acacaaaccc acctaaaata
     4921 tttcctaaga aacaatataa taaaatccaa gtcatcttct taacagaaac gactttataa
     4981 taccagccta ctgtaaagta catgaagtta cttgttaata attcagagtt agttaataca
     5041 actaaaatca aacctaaact aaacgcaatg gctcccatta aattgataag tccatctacg
     5101 tgagtactcg caaactgcgt ctttattccg aacataaata cagtaacgat tgagagtaaa
     5161 aatccggcca tcattgcttt aagcatatat cggccaggtg tttgctgtac cattacttgc
     5221 ttcatttgta ctgaagatga tattgaatca acaattgcgc ctgttgaata tgtatcttca
     5281 actgttttct tattttctat cacagtactt cccccattta ctttgatcaa ataaaattta
     5341 ctttctaatt atattagtaa atcaatgtaa ttgatagatt aaatttcaga aaaatctttt
     5401 tattctctcg aaaatgtagt tttctttcat aaaaacagcg agataaatcg cgtgattggc
     5461 taatatcttt atgatttaag atattacgac gctcatttta tctcgcttta tgtaaagtat
     5521 tacttattct tttttaaatt tgaaaagtaa tgtaaaccat atgcaaatac actgtaacca
     5581 attgcagtac gtaaaataaa ccttataact ttattaggta cacattttga cgttactgaa
     5641 atagaagata gtagtgttgt gaaaatatat aatattaaaa ctttgatata acgttctacc
     5701 atcgaatcat ccctcatttt tataatatta caagattatt ataaaccgaa tgaaacaaaa
     5761 aagtttttac atattttcaa caactttatt tcaataaagc atcaagttga tctaaagttt
     5821 gattcatacc ttgttcaaca cccatgttta tgacttgttg agcggcttct tttgttggaa
     5881 ataccgatgt tgatgtcact gtcgttttcg ttgtattgct cttggaaaat gacaaagtaa
     5941 ttttcatgct tggcattttt gtatcttttg caccttgagg tgtcgcaaat gagtcaatat
     6001 attcaattaa atatggacgc ttaaccgttt tatattctgc taatgtatag ctggtcattg
     6061 taggtgtttt aatcgcataa aatgcatcac cgcctgaaac agcattaaag cgaaacactt
     6121 tggtactagc gtcttttgga tgaaaccatt tttcaaataa tgcttttgtc gtataggcat
     6181 caaacacctt ttcaatcggt gcttctatcg ttcttgaaaa aataatttta ttatcttcaa
     6241 ctttaatcgt catcttccca ctccctatct tttatattta ctcatcttaa catgcattga
     6301 gtaacatatg ttaccaaatc atttgttata aactatagta atattaatca ttccctctgt
     6361 tgaaattgat aactacgaaa taaaaaacac actctattca gctattaaca gttgagtaga
     6421 gtgtgcaaac ctgttgaatt aatgcgctaa catttcttct ttgatttggt ctttatttaa
     6481 ttttgaagga taatatgttg gccagttatc catttctttt aataacgcgt cattatcatt
     6541 acccataaag atatggaagt gttctgcctt tttaggtgca atgttgtgat cgctaaattg
     6601 gatgaatttc ggtaagtctt tatcattacc atcgaccaat ttaaacataa atctcacgcc
     6661 acggttacct ttaggatatt tcaatgtttt cttaccattg tattcatatt taccagtgtg
     6721 tttcttacca tctttagtaa atgtaatttc atttcctttt atatcaatgt tagtaatatc
     6781 agttttatat cctttgtcat aataagcttt taaatcttta gcagatttct tcggatcatt
     6841 ttcagcttta tgttccatca cttcatcaag cgtaccgtct tttaagtaag ggtaaacaga
     6901 ttgccattca ccagcataat cgcttaattc acggtcttta acttgctcat ctttaaaata
     6961 cccatcagaa attgctttgt catgtttact ttcagctttg tcgtctttca ctttaacacc
     7021 actgtctaaa gctttaccga tattttcaat attagatttc attaatgatt gatacgtaat
     7081 attatctttt ttctgttgtt ctttatttaa agactccatg ttgtagaatt ttaaaggctt
     7141 cgcatctgtt tctttacgaa ttgtttcagt cactttattc gcaacattat cttcgtaaag
     7201 aatatattta gcattgctat ctctaatttc tttaacaatt ttagttaatt ctttttgtga
     7261 tggatcttca gcattcatgt tttgaatacc tttttgaaca aaaccataac aatcagctaa
     7321 gtatccaatt gattcatgtg aaatgaatac tgcattacct tgcttatctt ttgtaacttg
     7381 cttcatatcg ttatcaattt tcttaagatc gtcgtttaat tttttgtagt ttttctcata
     7441 gtcatcttta tgttttggat cttttttcac taattcatct ttaatttctt tagcgaaagt
     7501 ttggttaatt ttaggatcta accatacgtg tggatcatat ccaccatgat gatgatgatg
     7561 ttcttctttc tcatgatcat gtccctcatg ttcatgctct tcaccatgct cgtgttgatc
     7621 agttaaaagc tttgctttat ctaatttatc ctctaaagac agttttttat ctttatcttt
     7681 gatagtagat gcaactttct tagcaaccgg atctaaatta tcccctgtat acataaacaa
     7741 gtctgacttg cttgcactta atatatcttt ttgtgttggt tcatagctat gtaaatctgt
     7801 ccctgctgga tagattgatg ataccttcac gtgttttcca ccaatttgct ctgcaaatga
     7861 ttgcaatgga taaactgtcg ttttaattgt taccttgcca tctttatctt ttccatcatc
     7921 attcccacat gcggctaatg ataaagttac ggctggtaca agaagtaaca tacctaattt
     7981 ctttttcata gtcaccctcc taaatagtaa tctttacgtt ttaaaattat atactcatgc
     8041 tacttaaaat acaagcactg tttttataaa taatataaag tcgaatcata ctttaggtat
     8101 ttaccacttt tcgttttatc taatacatga ctattgtaca aatatgatac agaaaacttt
     8161 atagtattta cgaacgtcat aatataagaa aaaaagacaa tctctatcat attgctaata
     8221 gaaattgcct tttcatattg tcctaaaata acttacccta tttttatagt gtgactttta
     8281 gatttaataa tggtatttac atgatgctat ttttatatga ttgtcatcta ctgtataaac
     8341 caatctatgt tcgtgattga tacgtctact ataataccca gtcagattcg attttaactt
     8401 ttcaggctta cctatacctt ccaatgcacc atttctgtca atacttttaa gtaactcatt
     8461 aatcttcttc accatttttt tatcgttctg ctgaaaatac ttataatctt caaaggcttg
     8521 aggcgaaaac gtaatattta acctagccat tatacatcta tatctttcgt tatagttttc
     8581 ccacgttcta gatctgcaat tgattgagct aagtgttcag cattatttgg gttctgttgg
     8641 aggtaaagtg tttccatcat ggagttataa tctgattctg acatgattac tacgttttta
     8701 tcatcagtag atgttacagt taccataaca ctatcatcat ttacttttgt cataagtgcc
     8761 tttaaattct gtcgagcgta tgaataattt ttaataatca tatttatcgc ctccttttaa
     8821 cacacctcaa ttgtacaaat atctgtacaa taaaacaagc gacacagaaa aaaactacta
     8881 atcaagattt cgcacgggga agaatcaata cctatcctgt actgattccc tactaaacaa
     8941 tgtttacatg ataatttcca cctatttaga cttcatacat taaaaagaca atctctatca
     9001 tatcgctaat agaaattgcc ttttcgtatt gtcctaataa attggtatca cttactgatc
     9061 tcaacaatta agactatttg attttatctt ttaataactt ctcataactt tcataatcat
     9121 atggatcttc aactttctcg ccattaataa atgcagttgg tgtcgttttt atatgattat
     9181 ctttcgctat ttttttatct ttctctgcag ctttccaaga cttgctatct tttgtcttgt
     9241 aatcttttat aattttattt tccgtctctt tatctaaatg cagttgttta atatgtttat
     9301 ctaatagttc ttttgttaac cattctttat tttcatcttg ctgggcagca aataattgct
     9361 tttgaaaatc taaaaatgat ttaggtgcat acatcaatac tgcatgactc gcacgcgaac
     9421 caacaattga gtctttacct aagaaagcta aattgacaaa ttggtattcc actttgtgat
     9481 tatctatata atttttacgc aactttggca tgactttttc atctaattct ttacaataag
     9541 gacatttata gtcgccatat acgacaacta atggtttgcc gtttttcgaa gatgtcgttg
     9601 ctgattcttt tttaccgcaa gctgttaaaa ttaacatgct cactataaat aatgttagta
     9661 attttttagt cataataact cctattcata tctagttcgt catattttct tcatgataat
     9721 ccgcatcatg ttgttgataa tatcgtctcg aatcaaaatc ttgcttatat gatacacgtt
     9781 tatcactaaa atcatatgca taataatgca cttcatcatt actcaaaggt ttataagcta
     9841 aaatgataat tttatcctta tcatagacat aaatattagc atctttgcgt tcaaaatgat
     9901 caacacgatc accgttgcct tttttggcaa gttgttcttg tttttgattt tgaattttaa
     9961 ctgcttcatc tatttctttc acatatttat cattaccaca tcctgatata aagactagta
    10021 ataataatac cgtcgccaca aatcgcttca atgctcccac tcctactatc aacttccaac
    10081 taactttcaa tataattaat actatacacc gtcaaacatt aatgcgctac aactactacc
    10141 aatgcattta atataaatat ccaaagaaat aacagttatg tatagtcaaa gaaaaatata
    10201 tttcattttc tatgtgtatt tgtaatcagt ttttgataaa ataaaaaaat agcattatta
    10261 ataaattcaa aaattatagt tcaattcaga gagggatggg ataaaatgaa ctttgtaacg
    10321 ttgacttcgg atgagttcaa tgcgtttaca acaaagcatt tttcacatta cacacaatca
    10381 gctattcatt acaatcatag agttgattta aaaggcgatg tgcatcttgt aggggttaaa
    10441 gatgacaatg gtcaagtgat tgcaggatgc ttattgacag aagcacgcac acttaaattt
    10501 ttcaaatatt tttatacaca tcgcgggcca gtgatggatt atacaaatca atcattagta
    10561 gcatttttct ttaaagcatt aacgtcatat ttaaagaaac acaattgttt atatgtcctt
    10621 gtagatccat atttaattga aaatttacgc aatgcagacg gtgaaattgt taaatcttat
    10681 gataaccgag catttgttag aacaatggat aaattaggtt ataaacacca aggtttccct
    10741 gtaggttatg attcaatgag ccaaatccgt tggctgtcag tgttagattt aaaagataag
    10801 actgaagacc aacttttaaa agaaatggat tatcaaacga gacgtaatat taaaaaaaca
    10861 tatgatattg gtgtcaaaac taaaacgtta acgattgatg aaacgcaaac ttttttcgac
    10921 ttattccata tggctgagga aaagcacggt ttcaaattcc gtgagttacc atactttgaa
    10981 gaaatgcaaa agttatacga tgaccacgcc atgttaaagt tggcgtatat tgatttaaac
    11041 gagtatttaa aaacgttaca attaaagcaa caacaattaa cagctgaact ctcaggtgtt
    11101 gaagaagcat tggaagaaag tcctaattca aagaaaaata aaacgaaacg cacacagcta
    11161 gaacaacagc taaatagcaa taagcgtaaa atcgacaata caatagaaca aattgaacaa
    11221 gatggtgcag tcttaaactt agcttctgct ttatttatct acaatgagca tgaagtttat
    11281 tacttatcta gtggttcaaa ccctaaatat aatgcttata tgggtgccta ccatttgcag
    11341 tgggaaatga ttaagtttgc taaagcgcat catattgatc gctataattt ttatggtatt
    11401 accggtgact tttcagagtc atcagaagac tatggcgtcc aacaattcaa aaaaggtttt
    11461 aacgcacatg tagaagagta tatcggtgac tttattaagc ctatcaaacc attactatat
    11521 aaggtacaaa cgtacttaaa tcataaacgt cgttaaaata tcgctccaag attatgacaa
    11581 cataacaaag aaccacgcat catgtatatg gcttcatcgc catactaaaa catgatacgt
    11641 ggttctattt gattattctt catttataac atttaagaaa cgtcttaatt cttctgtttt
    11701 cggacgattg aacatctctt ctggagcccc ttgttctccg atcatgcctt catgaataaa
    11761 tacaatgtta ttagatactt ctttagcaaa acgcatttca tgtgtcacaa tgaccattgt
    11821 catgccttca ttagccaaat ctttaataac ctttaaaaca tcattcacaa gttcaggatc
    11881 aagtgcagat gttggttcat caaacaacat cactttaggg ttcattgcta gtgctcttgc
    11941 aatagcaaca cgttgttgtt gaccacctga taatgcatgt ggacgttgat ctttgacatg
    12001 tgttaaacca actttctcaa gtaactcaag tgatttccca cgtgcctcat cctttttcaa
    12061 ctttttaact gtgataagac cttccattac attttctaat gccgtcttat gcggaaaaag
    12121 gttataactt tgaaatacca taccagactg tttacgaact tctatttgtg attttttatc
    12181 tttagatgta tatgttttgc cgttaacata aactgtacct tcagttggaa tttctaatgc
    12241 attaatcata cgtaacaatg ttgttttacc tgaaccagat cgaccgatta aggttacaac
    12301 ctcaccttgt tcaacagata aatcaatacc tttgatgact tcaacatcat taaatgattt
    12361 atggatattg ttcaattgaa tcatgagcga taccctcttt caatataaga ttcatagata
    12421 ccttggataa ttgaaataat aaagcatacc acccagtaca ttagcgcaac taacacataa
    12481 attgttaaat attcatatgt tgtagacgca acttcttgag cttttctaaa catttcagcc
    12541 actaaaataa atcctaataa tgatgtatct ttgattaaac ttaaaaatgt attacctagt
    12601 gcaggaatcg acactcgaat tgcctgcggt aaaatgatac gttgtatcgt ttgaccatac
    12661 gtcataccga tggaatatgc agcttctgtt tgtcctttcg gtatagaaat aataccgcca
    12721 cgaataattt ccgaagcata cgcacctaca tttaatgata aaccaataat agcagccact
    12781 acaggtgcca atgtccattg gttgtcagcg tcatttgtta ataatctacc taattctgga
    12841 ataccataaa atatgataaa tagttgtact atcattggtg ttcctcgaat aatagataca
    12901 tagacacgtg aaatacttct taaaatttta ctcgttgaaa ttcgcattaa tgctgtaaat
    12961 aatgcaataa tcaatcctaa aacaaatgta actaatgtaa taggaattga atatttgacc
    13021 aaaccttcta gcataggtcc gaaagcttgt tttgcagcat ctagcgcatg ttgttgttcg
    13081 ctatttagat ttagaaacat cttgaccaaa ccatttctta cctattttag ctaattcacc
    13141 gttttcctcg atttttttca agccatcatt gaatttttga actgtttcat catctgcttt
    13201 tttagaaaat gcaaatgcag atctactttg ttcagcatta cctttgattg ctttgatctt
    13261 agcattaggt ttttgttttt tataatccaa gtatgacaga ctatcattaa atgtaccatc
    13321 aacacgctta gacaataata aatccattga ttggttaaag ccatcaactt ttgtaatatc
    13381 agcaccttta tccttagcta atttaccata attagatgtg aatgtttgtg ctaacttctt
    13441 acctttaaca tcatcaaaat ctttaatatc tttttcattt tcacgaataa ctaaaacagc
    13501 acttgagaat gtgtaaggct tagaaaattt atatttcttt tctctatcag gattaatacc
    13561 tacttggttc gcgattacat caaaacgccc tgcgtctaaa cctgcaaaca tagaatccca
    13621 agaagtttca ttaaatttaa gttttaaacc ttcttcttta gccactgctt taataacatc
    13681 aatatcgtaa ccagttaatt taccatcttt atcgtggaaa ctaaatggtg cataagtccc
    13741 ctcagtccca acattaattg tcttgctatc tttactagct tccttgtctt tcgacgaatt
    13801 gtttccgcat gcagccaata tgaaaacgaa agctatcatc acaaataaaa gtcttttcat
    13861 agaatatatc tccttattct tattattcta atcggaataa aaatatttta atctgaaatg
    13921 ccggataagt caatatgttg aacttttgct aaaaagcttt atatagctag caacttatat
    13981 ttattgacga gaatcaactt cttttttctt accttgtaac ataaatgaac aaaagaaact
    14041 aacaactgta atcaataatg caaataaaaa tccagcgtga taaccatgca acatcgtatc
    14101 aactttaatt tgattcatca tatcaatttt gttcatgcca ttatagtctg acatagttgg
    14161 agaaaagttt ttgcttgcat gggataaaat agtaattaat gcagctgttc cgatagaacc
    14221 agcaatttgt tgtactgtgt ttgtcataga tgaaccatgt gcattcattt ctggctttaa
    14281 ttgattcatc gtatgggtca ttactggaat taatcctaac gcgataccaa ccattcgaat
    14341 cgcataaact gttgccaaca tgattgttga tgtttgttca tccattacaa caaaataagc
    14401 cgtagtaata acaacaatcg ccatacctac taaagcaaga tttctaggac caaacttttc
    14461 atataaagca cccgaagtca tcgacataaa tgccataaca attgctcctg gtaataaagt
    14521 taatccagaa tctaaagctg aacgatgcaa taaattttgc acaaagatag gtaaaatcgt
    14581 ttccgaacca atcatcgata ccattgtgac acccataata atgataccaa ctgtaaattg
    14641 tttattttta aatacagcaa aatttaataa tggtgttgat agccgatatt gacgtaatac
    14701 aaataatgcg actaaaacaa cgcctccaat catagatact aatacgatag gattatcaaa
    14761 acctttttct gaaattgaac tcgttccata caataacccg ccgaaaccta gtacagaaaa
    14821 aatgacagaa ataaaatcaa taggtacttt aattgtagtt ccaacatttt ttatcgtttt
    14881 aaatccaatt aaaaatgtca cagctgcaat tggagcgaca acatggaaaa gcgatctcca
    14941 gttaaaatat tcaacaaggt aacctgaaag agtaggtcca atagctggtg ctaaaccaat
    15001 taccaaacca aatatcccca tatatttacc gcgttcatgt ggttggaaaa catccaaaat
    15061 cgttgtcatc atcaatggca tcataatacc tgcgcctaac gcctgaataa ttctagcaac
    15121 taataatgtt gcaaagtgcg gactcaagcc gccgagtaat gtacctaaga aaaagataga
    15181 aatacccact aaaaacactt gtcttgttgt ataacgttgt ataacaagcg cggacaaagg
    15241 aataacaatc ccattagtca ataaaaaagc tgttgtcagc cattgaactt gtgtatatga
    15301 aatttcaaaa tctttcataa tacttggtaa agccgtcact aatagtgttt catttaataa
    15361 tccaaaaaat ccacccatca gcatagtaat aatcatcgtt aatttttgtt tcttcgacat
    15421 ttaaagcttc cccctcacca tctaaaaaac gacacacatt tttagatatc cggtaattat
    15481 acccattttc caaaaaatta gcacgacttt attacaattt ctttaaaacg taatcattac
    15541 tatttacata tgagtagtta tcggtcataa tatatttaca aggagggata gagatgaaat
    15601 tagatttaca gactgctcgt cgtaatttaa atagccctaa cattaaaaca agaaagcgtg
    15661 ctttaaagat tattaagcaa cataaaagag cgaaataatt gttttaattt gactatattg
    15721 tatgtctaac tatattttcg agtatcccta gctcgttaat ataaactcta tacttaacta
    15781 tttccttaca ttacttcaca tatttaagag ctgcatatac actcactcct tgtgtacatg
    15841 cagctctttt tttataagta gtatttatct ataacttcta aatcatccgt taattcataa
    15901 acaagcggtg cacctgtttt aatttcataa ttaatgatat cttcatctga cacatcttca
    15961 agatatttaa tcaatgcgcg aattgaattt ccgtgtgcag aaactaatac cgtttgacca
    16021 tctagcaaat attgtgaaat atgatctgtc caaaatggta tcactcgaac taaagtatct
    16081 ttcagacttt cagaataagg catcatacgt ttatctaaat gattatatcg acgatcagct
    16141 aagtaagctt cacgttgttc ttcggtttca gcaggtggtt tcacatcata agaacgacgc
    16201 caaatatgta cttgttcttc tccaaattct tttctagcat catctttatt taagccttgc
    16261 aatccaccat agtggcgttc atttaaacgc cagcttttat atacaggaat ccattgttgt
    16321 ttagattcag ttaaaatata atgcgttgta tctaaagcac gtgttaataa cgatgtaaaa
    16381 gctacatcga tggcaatgtt attttctctt actttttcac ctgctctagt cgcttcatta
    16441 ataccttgtt cagataaatt aacatcttcc catccagtaa ataagttttt agcattccac
    16501 tcgctttgtc catgacgaca taaaattaat tttggcataa cttataacct ctttccttta
    16561 atctttagga cgttatttca gggatatttt aagatgattt aatcttagta attttctttt
    16621 caatatattt taaaattcaa tgattaaatc aattatcact agacattaaa tacataaatc
    16681 ctattcccca ttttcatttt ttaattcata aatgaatcaa taaccaccta ataacaaatc
    16741 atattataca cctttgttcg ctatttttct aaggtttaaa aaatattttt aggtaaacct
    16801 aaaaatagat gtaataaaaa cgcctcctca gatatttata tatctatgaa gacgtttaaa
    16861 tacattatag atggtctggt tctgggtgaa cgtatactga ggaaatacct tttttgtgca
    16921 aatgatgttc gacattgtca caaatttgat gcgcttctac taaggaaagg ttagcatcta
    16981 caacaattgt gacatcaata aacacactac ttccatggta acgcccttta atacttttaa
    17041 cttcttgtac ttcatcaact tctaaaatat cattgcgata cgcttctaat tcagtttcat
    17101 tgaaaccatc actcaacata aaaattgctt ctttaaaaat accaaaacca gtataaacga
    17161 ttagtaagcc tagtaatgtt gctaaaataa tatcgacaat tgggaaaccg atttgcgtaa
    17221 aaattaatcc tatcgctgtt ccaatgctga ctaaactatc cgataaatta tctttggcag
    17281 ccgaatttaa agaactactt ttcgttcttt tcgctagtct ttgattgact gcaaatacaa
    17341 tcaacattac aagaccactg attaagctga cgataattgt tattgcgtta ggtacaacgt
    17401 catcttcttt gaacaaacga ggtgcatttt gaataactac ttggatacct acaaacataa
    17461 tgacaaatga caccaataat gaagaaatat tttcagactt caaatggcca taaggatgat
    17521 ttcgatcggc aggtttaatt gaaattttca atccaataat aacagctaaa gaaacgataa
    17581 tatctgtcat attgtttaat gcatcggctc ttacagctgc agagttaaag acaaaacccg
    17641 tgacatactt aacaatagat aagattatat atacaattaa actcaaataa gcaccgcgtt
    17701 gcgccaattt aagattttca ttatgagaca tgcgttgaac caccttgaat tagtatagta
    17761 acaatattat gaatgattca ttttaatttt acaacgtttt taatttttat aaatttttat
    17821 aaaattaaac taatttattc attcgcaacc cctaaaaata atttttagcc tttctgcgaa
    17881 ttttatgagc tagaaaggcg cccaactctc cctgtttgtt aactttcgcc tcgaaagttt
    17941 ctatgttggg accctatgca ttagtagcga agctatctaa tgtgttcctt tattttctgc
    18001 gaattttatg agctagaaag gcttatgcag ttgacgtttt acgtccaact gcgttcctcc
    18061 gtcttcttca aatttatttg ttagaaaggc acccaactct ccctgtttgt taactttcgc
    18121 c
//
LOCUS       DANRDI010000041        17691 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.041, whole genome shotgun sequence.
ACCESSION   DANRDI010000041 DANRDI010000000
VERSION     DANRDI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.041"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(74..1351)
                     /locus_tag="PZ377_001105"
     CDS             complement(74..1351)
                     /locus_tag="PZ377_001105"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDM6357986.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            1605..2771
                     /locus_tag="PZ377_001106"
     CDS             1605..2771
                     /locus_tag="PZ377_001106"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDM6357987.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(2941..3462)
                     /locus_tag="PZ377_001107"
     CDS             complement(2941..3462)
                     /locus_tag="PZ377_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDM6357988.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            3871..5976
                     /gene="clpL"
                     /locus_tag="PZ377_001108"
     CDS             3871..5976
                     /gene="clpL"
                     /locus_tag="PZ377_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDM6357989.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(6045..6215)
                     /locus_tag="PZ377_001109"
     CDS             complement(6045..6215)
                     /locus_tag="PZ377_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDM6357990.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(6228..8222)
                     /gene="feoB"
                     /locus_tag="PZ377_001110"
     CDS             complement(6228..8222)
                     /gene="feoB"
                     /locus_tag="PZ377_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDM6357991.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(8234..8461)
                     /locus_tag="PZ377_001111"
     CDS             complement(8234..8461)
                     /locus_tag="PZ377_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDM6357992.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(8677..11166)
                     /gene="farE"
                     /locus_tag="PZ377_001112"
     CDS             complement(8677..11166)
                     /gene="farE"
                     /locus_tag="PZ377_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDM6357993.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            11311..11859
                     /gene="farR"
                     /locus_tag="PZ377_001113"
     CDS             11311..11859
                     /gene="farR"
                     /locus_tag="PZ377_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDM6357994.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(11936..12103)
                     /locus_tag="PZ377_001114"
     CDS             complement(11936..12103)
                     /locus_tag="PZ377_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6357995.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(12215..13759)
                     /gene="pruA"
                     /locus_tag="PZ377_001115"
     CDS             complement(12215..13759)
                     /gene="pruA"
                     /locus_tag="PZ377_001115"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDM6357996.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(13949..14548)
                     /locus_tag="PZ377_001116"
     CDS             complement(13949..14548)
                     /locus_tag="PZ377_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDM6357997.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            14787..14978
                     /gene="cwrA"
                     /locus_tag="PZ377_001117"
     CDS             14787..14978
                     /gene="cwrA"
                     /locus_tag="PZ377_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HDM6357998.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            15218..17626
                     /locus_tag="PZ377_001118"
     CDS             15218..17626
                     /locus_tag="PZ377_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDM6357999.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT         5779 a         3320 c         2826 g         5766 t
ORIGIN      
        1 gttcatatta atttatctaa gcgctacgat ggactgactt aaacttttct ttaaaatcgc
       61 tattcgccat ttactattgt tgtctaattt cttgtaaaat atgttccgct gcttgtgtat
      121 ttgcacgggg ttcttttttc aaagcttcag ctactttagc aatttcatca ccttttgccc
      181 ctacaacgat agctaatgat ttatattgta aactcatatg accttgttga ataccttctg
      241 acacaagcgc gcgacatgct gcaaagtttt gcgctaaacc aacggcagca actacatgac
      301 ctaattcttg tgctgactct acatttagta gctctaatga agctttagca attggtaata
      361 cttttgtacc accgccaacg attgccaatg tcataggcac ttcaattgta ccaatcaatc
      421 gttgacgatc ttgatcgtaa cgccatgtag caataccacg atactgtccg tcacgactcg
      481 cgtatgcatg cgcacttgct tctgcaccac gcgtatcatt tcctgttgct aaaacaacag
      541 catgtatgcc attcataaca cctttattat gtgttgctgc acgatgaata tctacttggg
      601 ccaatacaga agcacgttcc attcgtttgg caacctcttc tccagttctc tcgccccttg
      661 ctaaatcttt aacatcaatt tcgccttgaa ctttaacaac ggacgctgtt gcatgattgg
      721 ataaaatact cattaaaatg tcgctttgcg gaaattcatt ttttaaaaat gcagttatgg
      781 cctctaaaat cgtattaagc atattagcgc ccatagcatc tttcgtatca acaaatactt
      841 ttaaagatag taactgttgc tcaggaaatg tatcaatcgc tatacgttgg taaccaccac
      901 cacgcgcttt aatagaagga tatgcctcat ccgcaatttt atgaatttgc ttttctaaag
      961 ctttaatgtc tgctgataat ttttcagtat cgtcaacgcc atcaaagacg atttgaccta
     1021 tcataatacg ttcagaagat accgttttaa atccgccagt ctgattcact agctttgcac
     1081 cataactagc tgcagcgaca actgaaggct cttccaccat cataggtaca acatatgcct
     1141 tatcgtccac aatgatattc ggtaataatc caacgggtaa tgcaccttgc gcgatgacat
     1201 tttcaattaa actattggct acttcttcat cgattaatgg atgattcagt aaaatgtcga
     1261 attgttcttc tgataaccat tgcttatcaa ccaattgttg taacttttct ttacgagata
     1321 aatgtcgaaa attcttatct aaattttgca tggacgtact ccttttactt cacataattt
     1381 ttaacatttc aatcactact atttttacca caaaataacg tcattcgtct taaaattcaa
     1441 ttgaataatt gtcgttttga ctttaaaata aaacaaggta aattaaaacg cttacaagaa
     1501 acgacaaatc atttttaaat ttagtatatt tctttgtata aaattagcat attctgatat
     1561 gatacaagtg ttgcttttat aaatttgaaa ggatgtaaaa ccttatgaca ataggtatcg
     1621 ataaaataaa cttttacgtt ccaaagtact atgtagacat ggctaaatta gcagaagcac
     1681 gccaagtaga cccaaacaaa tttttaattg gaattggtca aactgaaatg gctgttagtc
     1741 ctgtaaacca agacatcgtt tcaatgggcg ctaacgctgc taaggacatt ataacagacg
     1801 aagacaaaaa gaaaattggt atggtaattg tggcaactga atcagcagtt gatgctgcta
     1861 aagcagccgc tgttcaaatt cacaacttat taggtattca accttttgca cgctgctttg
     1921 aaatgaaaga agcttgttat gctgcaacac cagcaattca attagctaaa gattatttag
     1981 caactagacc gaatgaaaaa gtattagtta ttgctacaga tacagcacgt tatggattga
     2041 attcaggcgg cgagccaaca caaggtgctg gcgcagttgc gatggttatt gcacataatc
     2101 caagcatttt ggcattaaat gaagatgctg ttgcttacac tgaagacgtt tatgatttct
     2161 ggcgtccaac tggacataaa tatccattag ttgatggtgc attatctaaa gatgcttata
     2221 tccgctcatt ccaacaaagc tggaatgaat acgcaaaacg tcaaggtaag tcgctagctg
     2281 acttcgcatc tctatgcttc catgttccat ttacaaaaat gggtaaaaag gcattagagt
     2341 caatcattga taacgctgat gaaacaactc aagagcgttt acgttcagga tatgaagatg
     2401 ctgtagatta taaccgttat gtcggtaata tttatactgg atcattatat ttaagcctaa
     2461 tatcattact tgaaaatcga gatttacaag ctggtgaaac aatcggttta ttcagttatg
     2521 gctcaggttc agttggtgaa ttttatagtg cgacattagt tgaaggctac aaagatcatt
     2581 tagatcaagc tgcacataaa gcattattaa ataaccgtac tgaagtatct gttgatgcat
     2641 atgaaacatt cttcaaacgt tttgatgacg ttgaatttga cgaagaacaa gatgctgttc
     2701 atgaagatcg tcatattttc tacttatcaa atattgaaaa taacgttcgt gaatatcaca
     2761 gaccagagta gtcggtgtat ttaaaacaca tataataaaa cctaaaagca gcagtaagac
     2821 cacttctaat tgaaatcgtc ttactgctgt tctctattta taacacttcg tattgaatga
     2881 attcattatg cctatttgac acattattga agttttccta atgcctggat cctttatacg
     2941 ttacggcttc gtgctatgtt ttggtacata aagctttgac atatcgatat tctccaactc
     3001 taacagctta attttattat taatcgttcc accgaaccct gttaagctac ccgttttacc
     3061 gacaacacga tgacatggca cgataataga taatggatta cttccgactg cacctccaac
     3121 cgcttgggct gacatttttg gcttgttaag cagcttgcct acttttttgg caatagcacc
     3181 atacgttgtt agagtcccat aaggaacctg tcttaattca ttccaaacac actgttgaaa
     3241 atgactacct gttggcttta aaggtattgt gatttcagga ttgtcacctt taaaatacgc
     3301 gtctaaccac tgtgtcgcct ctctaaatat cgctaaagac gtattttctt ccctagtacc
     3361 atcaccttgt tgattttcaa acaaaacagc ggtcagactt accccatcac tcaaaagttc
     3421 caatcgtcct acaggcgaat catagtaact cttatactcc ataaaaatcc cccctttttc
     3481 taatcatacg ccttaattgt taaattagaa aatattcatg ttcacatttt ttaaaaataa
     3541 ataagtcgtc aattacgtat ataaacacgt aataccagct atcactttgc tgcaatatac
     3601 agttacatat cttactacac gtgctaacct cttactttgt aaaccaaatc ttaaattaaa
     3661 atattgaaaa tgcaatgaat ccttaatatt ttattaaacc tataattact tattaaaaat
     3721 aacacacaat attcataaag ttttaaaaat attctgtttt atcacctact attagtggaa
     3781 aagtacaatt gcaattgtat atagtttgca taacgcttca aaagtaattt cttttttgtt
     3841 tagttcaaaa aaatttagag gtgatgttat atgaataacg gttttttcaa tagcgacttt
     3901 gattcaattt ttcgaagaat gatgaaagat atgcaaggtt caaatcaagt cggaaacaaa
     3961 aagtactata ttaatggtaa agaagtttca cctgaagaac tagcgcaact cacacaacaa
     4021 ggtggcaatc actctgctga acaaagtgcg caagcttttc aacaagcagc acaaagacaa
     4081 caagggcaac aaggtggcaa cggcaattat ttagaacaaa ttggtcgtaa ccttacgcaa
     4141 gaagcacgtg acggtttatt agatccagtc attggtcgtg ataaagaaat tcaagaaact
     4201 gctgaagttt taagtagacg aactaaaaac aatcctatat tagttggaga agctggtgtt
     4261 ggtaaaactg cgattgttga aggtttagca caggcaatcg ttgaaggaaa tgtaccagca
     4321 gcaatcaaag acaaagaaat tatttctgta gacatttcat cattagaagc tggaacgcaa
     4381 tatcgtggtg cttttgaaga aaatattcaa aaattaatcg aaggtgttaa atcttcacaa
     4441 aatgccgtac tattctttga tgaaatccat caaattatcg gttcaggtgc cacaggaagt
     4501 gattcaggta gcaaagggtt atctgatatt ttgaaacctg cattaagtcg tggtgagatt
     4561 tctattattg gtgcaacaac acaagatgaa tatcgaaaca atattcttaa agatgctgca
     4621 ttaacgcgca gatttaatga agtgcttgtt aatgaaccaa gcgctaaaga tactgttgaa
     4681 attttaaaag gtattcgcga aaaattcgaa gaacaccatc aagtaaaatt accagatgac
     4741 gtattaaaag catgtgttga cttatcaatt caatatattc cacaacgatt attaccagat
     4801 aaagcaatcg atgtgttaga tattacagca gcacatttat ctgcgcaaag tccagctgtc
     4861 gataaagttg aaactgaaaa acgaatttct gaattagaaa atgataaacg taaagcagta
     4921 agtgctgaag aatataaaaa agctgacgac attcaaaatg aaatcaaatc attacaagat
     4981 aaattagaaa atagtaatgg tgaacatact gctgttgcta cagttcatga tatttcagat
     5041 actattcaac gattaactgg tattccagtt tctcaaatgg atgataacga tattgaacgt
     5101 ttaaaaaata tttctaatcg tttaagaagt aaaatcatag gtcaagatca agctgtagaa
     5161 atggtttcac gtgcaattcg ccgtaatcgt gctgggtttg atgacggcaa ccgtccaatt
     5221 ggcagtttcc tatttgttgg ccctactggt gttggtaaaa cagagcttgc taaacaatta
     5281 gcaattgatt tatttggtaa taaagatgca ctgattcgac ttgatatgag tgaatatagt
     5341 gacacaacag ctgtttcaaa aatgattggt acaactgctg gttatgttgg ttatgatgac
     5401 aattcaaata cgttaactga aaaagtacgc cgtaatccat actcagtcat tctatttgat
     5461 gaaatcgaaa aagcaaatcc acaaatttta acattgttat tacaagtaat ggatgatggt
     5521 aatttgactg atggtcaagg taatgtcatc aactttaaaa atacaattat tatttgtaca
     5581 tcaaatgctg gctttggcaa tggcaatgac gctgaagaaa aagatattat gcacgaaatg
     5641 aaaaaattct tccgccctga attccttaac cgcttcaacg gcatcgttga attcttacat
     5701 ttagataaag atgcattgca agatatcgtc aacttattat tagacgatgt acaagttaca
     5761 ttagacaaaa aaggtattac gatggacgtt tctcaagatg cgaaagattg gttaattgaa
     5821 gaaggctatg atgaagaatt aggtgcacgt ccattaagac gtattgttga acagcaagta
     5881 cgtgacaaaa ttacagatta ctatttagat catacagacg ttaaacatgt ggatatagat
     5941 gttgaggata acgaattagt cgtaaaaggt aaataacgac actttaacat atcgcgcatc
     6001 aaaaatgagc atcaggtcgc ccttgcctgt gctcattttt ttaattattt ccctggaaaa
     6061 tgattcgctg tgtgctgttc tgttccacaa caatcacgat taatgtcaca tgtaccacat
     6121 tttccttgtt ttgaacgctt gaaaaatttt actagtgtat ataaggcata tccgaaaatt
     6181 gctaaaaaaa ttaaaatgtt aataatgact gacactttaa ccactcctta aacaaataaa
     6241 tgtccgactt gataaaaaat gaatgttaag acatatgcag tgactagagg ataggcaact
     6301 gcaagtgccg tccatttcca tgaataagtc tctttacgga ttgctgctac tgtagaaaca
     6361 caaggaatat acaatagtat aaatatcata aatgcatacg cagatagcgg tgtgaattga
     6421 ttttgaatca cattaacaag gcctgcatca cctgatgaat agataatcgc catcgaactt
     6481 acgataactt cttttgctaa aaatcctggc actaacgtag cacctgcttg ccatgttcca
     6541 aatccgagcg gttgcactaa cataccaaag aaactaccaa ccatatgtaa aaaactttga
     6601 ttgatattca cattgatacc atgtggtcct acataactta atagccaaat gactactgag
     6661 ccgccaaaaa taaatgtacc tgctttacga acaaagccct tagccttttc ccaagtacta
     6721 cgccacaacg ttttaatgga aggcacacgg tatgttggca attccacaat aaagattgca
     6781 ttatcatttt ttaaaatcgt cttagtaagt actgtactga ctaaaaatgc cataataata
     6841 cctaaaacat acaggcttaa tactactaaa gattgattct ctttgaaaaa gatacctacg
     6901 aacaacgcat acactggcag tctagcagag catgacatga atggtgcaat taatatcgtt
     6961 gttaaacgct ctttttcatt ttcaatactg cgcgcagcca taatactcgg tacattacaa
     7021 ccaaatccga taatcattgg tataaaagac ttcccgctta aaccgaacga ttccataata
     7081 cgatccatta aaaccgcaat acgtgccata taacccgaat cttctaataa agatataaag
     7141 aaaaagagca caacaatttg tggtacaaag actaatactg atcctacacc agcaataatg
     7201 ccatctgtaa ttaaatcttg taaaaatggt ataacaccaa gataattcat aatcgtcttc
     7261 acactatctg taaatgtacc acctataaat gcatcgagtt gatccgacaa aggtgtgcca
     7321 atccatgtaa atgtagtttg aaagatcaac cacataattg ctagaaagat aggcatccct
     7381 atatatttat gtgttaatat cttgtctatt ctagagctga aatattgctt atcttcatct
     7441 ggatacgtta ccacgtcttg caataacgtc tcaatataat gattgcgtat acgctccatc
     7501 tctcgacgaa cagatacagc ccctacttgt tcagcaactt gatcacgtaa actcgacaat
     7561 ttatttacaa cctctgaatt aagttcgttt gcaatttgca tgttatttaa taagaattga
     7621 atcgcaataa acctagcttg atacttatca tgagatgtct ctgtcattat tatttgacac
     7681 atatttttaa ttgtctcttc aatcttctca ccataattga ttttaaaatg cggttgatac
     7741 ccttccccta gatgctttat ttcgccaagt aaatattttg ttcctttgcc tgtacgtgcc
     7801 acaactggaa aaataggtgt ttttaacttt ttcatcaatt tatgataatc gatttttatc
     7861 ccgcgctttg tagctacatc aatcatattt aatccgatgt atattggttg attaagttct
     7921 aacaattgta ctgttaattg catatttctt tttagttgac tcgcatcaac aatgttaatg
     7981 attcctgaaa atgaatcgtt taataaatag tctgtcacta cagtttcatc tttagaaatc
     8041 ggcgataaat catatgtacc tggtaaatca attaattgtc ctacattttc tttaagtttc
     8101 cctacttttt tctctaccgt tacgccactc cagttgccta tatattcata cgaaccagtt
     8161 aaagcgttaa acaaagatgt tttaccaaca ttaggatttc ctaaaataca ataattttcc
     8221 attcgtccgg ctcctattct tctaatgcaa tagaacaagc atcgcaatgt ctaatactta
     8281 actgttgtcc gtttacttca ataatacatg gccctttaaa taaacatttt tgtttaatcg
     8341 ttatgatagc gtcatctgtt aacccaaagg cacttagacg atacaacata ttctcattag
     8401 caatatccat tcgctttatt ttataagcct tattcatttc accattttta atgtttaaca
     8461 tactatttcg ctctcctatt agaaataata atcattatca cttaaaaatc ataaccctta
     8521 aaattgtagc tcgcaatact ttatttaaat aattttcatt tttcatgtaa aatttgtgac
     8581 attgcaaaaa tgttttgtat aatcacttcc atgcaaaaaa cctccgcttc aaatgtcatt
     8641 gatagacatt taaagcggag gcatgtacat gttattttat gatttatttc gtagattttt
     8701 ttctaacaat tgattgagct tttcaatatt tttattcaac tgttctatat tcgttgcttg
     8761 tttttttgaa tttagttgat gcttgttttg ataatctatt aaagcatcat ataagaacaa
     8821 tgaacttgct gattgctgtt tcacttcttg gtacaatgcc atcgttttat catctagtac
     8881 tattgaatgt gcatcatctt gcccatgtgc atcttgatgt ttcgatacat ttgttgcacc
     8941 ttgtggatat actttaaagg ttgtgtcgta tgtttcctta ctatctttag cttcagattc
     9001 ctgtgattca accgttttat atttttcaag tgcatgtcct tcaatatcaa ctcgtggaat
     9061 aatgcgattc aaccatgctg gtaaatacca cgaacctttt ccaaacaatt tcgttaatgc
     9121 aggaattaac atcattctga ctacgaaggc atcaaagagt acaccaaacg ctaatgccat
     9181 acccattgat ttaatcatga catcttcttg gaatacaaac gcaaagaaga cactaaacat
     9241 aattaatgca gctgctacaa taacaggacc gctttctttc aatcctactt tgatagaata
     9301 atcattatcc cctgttttac tatattcttc atgaattcgc gacataagga agacttcata
     9361 atccatcgct aatccaaata agatacctat agtaataacc ggtaaaaatg ctagcattgg
     9421 tcctgtcgtt tcaataccaa acagaccttt cataaaacca tcttgcatta ctaatgttgt
     9481 aaatcctaat gttgccatta atgacaagac gaatcctaaa actgctttta atggtattag
     9541 aattgaacgg aagacaatca ttaataagaa aaatgctaat acaacaatga ctgaggcaaa
     9601 taaaggtatc gcctcattta actttttaga catatcaata ttaatgacac tttgtcccga
     9661 aatctccgtt ttgaacccat atttatcttg tgcatcttta tgataatctc gtaaatcatg
     9721 cactaaatca tttgtactct ctgcattagg cccttgctta ggtatcacga ccatcaaagc
     9781 gtaatcatta tctttactca tttgtggtgg cgtaacgata tctacatttt tcttatcttt
     9841 aatatcttta tatacagact gtaaatcttg ttgtaatcct tgtggatcat cctttttatc
     9901 tttcacattt atcaacatcg gtatttggcc attaaatcct tcaccaaatt tatccgagat
     9961 aatatcgtaa gcttttttct gtgtagaatc tgctggttta acaccgtcat ctggaatacc
    10021 aagtcgcata tgactaactg gtattgcagc tgctactaat atgattaaac ctagtaatac
    10081 tgccgcaagt gcatttcctg taataaattt agaccatggc gtatcaatat cttttttgaa
    10141 tttagactgt aatttattca ctttaatgcg tttatggaaa atgcttatta atgcaggtaa
    10201 taaagttaaa gcgctaagta ctgcaaaaac aacactaatt gccgaagcaa atcccattac
    10261 cgctaagaag tcaatgccta ctaatgataa accacatact gcaattacaa ctgttacacc
    10321 agcaaaaaca actgcactac ctgctgttcc tattgcaaga ccaatgcctt taatgtaatc
    10381 tgtttcagtt ttcataactt gtcgatatct gaataaaata aataatgcat aatcgatacc
    10441 aactgctagt ccaatcatta cggctaatgt cagtgtgaca tttggtatat cgaatgcata
    10501 agttaacaaa ctgataatac ctacaccaga ggctagacca atcaatgcac ttataattgg
    10561 taatcctgca gcaatgactg aaccgaatgt gattaacagt acaacaaatg caacaataat
    10621 accaactagt tcagaattac cgcctacttc tgtagatgtc attcctgtac cagttaactc
    10681 tgtttgtaca ttatgattgt cacgtaagtc tttcaaatga cttttaacat tatctctaga
    10741 gccatctttt aaagatgttt gactaacgtc atatgtgata tctgcaaatg cagttgtttt
    10801 atctttacta atttgcttat tttcataagg atctgatatt ttatcaatgt gcttgtcatc
    10861 ttttttaata tcatctaacg ttttcttaat atctttagta atgttcggtt gcacaatacc
    10921 atcatcttta gtcgtcttaa agacaacacg tatttgtgcc ttttcactat cttgattaaa
    10981 atgtttttca atctttttat tcgtatctaa cgactctaat cctgtcattt taatatcatt
    11041 gtcaaatttc ggtgcatttg tagcaagtgg tatcaatatt gcagctacaa tcactatcca
    11101 tgcaatgacc gcggaccatt tatgttttgc gatgaatgtc cccatcttat ataaaaattt
    11161 tgccaaagta tattgcctcc ttttaaaatc aacgttatag tttaaatata cagtgtagat
    11221 tattgttcga ttatagtatc tatccccgac ctcttaaaga atcaattgga aaattttgta
    11281 tattaaacta cacacaaagg agaaatgtag atgaaagaga ctgatttacg agttataaag
    11341 acaaaaaaag cattgtcgag tagcttgcta caattgttag aacagcaatt attccaaacg
    11401 attactgtca atcaaatttg cgacaacgca ctcgtacacc gtacaacatt ttataaacat
    11461 ttttatgata aatatgatct tctagagtac ttgttcaatc aattgactaa agactacttt
    11521 gctagagata tcagtgaccg tcttaatcat ccattccaaa cgatgagtga tacgattaat
    11581 aataaagagg atttgagaga aatcgcagaa ttccaagaag aagacgctga atttaataaa
    11641 gtattaaaaa atgtctgcat taaaattatg cataacgata tcaaaaataa tagagaccgt
    11701 atcgatattg acagcgacat cccagataat ctcatatttt atatttatga ctcgttgatt
    11761 gaaggtttta tacattggat aaaagatgaa aaaattgatt ggcctggcga agatattgat
    11821 aacattttcc atagattaat caatattaag attaaatagt agatgagaaa ctcatgagcg
    11881 ttaccaacat tcataataaa aacgatagtg tacacgttaa tgaattcgtg tactactatc
    11941 gttttttatt tttatcgtgc ttatcgctat taaaacaact gatacacaac acataaacta
    12001 tgaagaaaaa aataaatccg ctatctaaat gactttgact cagttgttta aatgaccaaa
    12061 ttgctaatac aattcccatt attattgaaa taacgtatct cacattctta tacctataat
    12121 ccttttctaa aaatatggtt gctattactt aatttttaaa gttataaata aaaagagcca
    12181 accgcaatgg atggcccttg ttcattatga agcattagaa catttctgaa acaacctttt
    12241 gttctaagaa gtgtaataag tagtctggac tacctgtttt agcgtccgta cctgacattt
    12301 tgaaaccacc aaatggatgg tatccaacaa ctgctgaagt acagcctctg ttaaggtata
    12361 aattgcctac atcaaattcg tttaccgctt taatccaatg ctcgcgatta tttgtaatca
    12421 ctgcaccagt taaaccgtaa tctgtatcat ttgcaacctc aattgcttca tcaaaatcgt
    12481 taactttcac aaagccaaca actggaccaa aaatttcttc ttgcatgatt ctatctttag
    12541 atttaagtcc tgaaatgatt gttggttcta caaagtaacc ttttgaatca tcagtgccgc
    12601 caccttgttc taatttacct tcttctttac caatctcaat ataattttta atcttatcaa
    12661 attgtttttt attaataact gggcccatat acgtattgtc tacagtattg cccaacgtta
    12721 attcttttgt taatttgatt gatttctcta atacttcgtc ataaacgtct ttatgcacaa
    12781 ttgcacgtga acatgctgaa catttttgac cagaaaaacc aaatgctgac gttacaatag
    12841 cttctgctgc catatctgta tcaatatttt catcaactac aatggcatct ttaccaccca
    12901 tttcagcgat aacacgtttc aagaagtttt gaccttcttg aacaacggca ctacgttcat
    12961 aaattctagt acctgtcgca cgtgatcctg taaatgtaac gaaatgcgta tctttatgat
    13021 caactaagta atcaccaatt tctttcggat caccaggaac aaagttaact acgccttttg
    13081 gtaatcctgc ttcttctaaa atttccatta atttataagc gatataaggt gtatcctcag
    13141 caggtttcaa taacactgta ttacctgcca caactggtgc taaagttgta ccagccataa
    13201 tcgcaaacgg gaagttccac ggcggaattg taacacctgt accaattgat ttatagaaat
    13261 atttattgtg ttcaccttca cgatcaagta ctggcttacc ttgagccaag tccatcattg
    13321 aacgtgcata gtattcaata aaatcaatac cttcagctgc atcaccaact gcttcatccc
    13381 atggcttacc tgcttcataa accataattg ctgcaatttc cgcttttcga cgacgaataa
    13441 ttgccgaaac acgtaacata agctctgcac gatcatttgc tgaccatgtt ttccaagatt
    13501 tataagcttc gtttgctgct ttaaacgcat cttcaacatc ttgttttgtt gcctttgatg
    13561 catttgcaat cacttgtgat gtgtctgcag gattgattga tttaattttg tcatctttga
    13621 aaatcttctc tccattaatc actaatggta tgtcttgacc taattctttt tccacgtctt
    13681 tcaatgcttt cttaaacata tccacatttt cttggactga aaaatcgtaa ccaggttcat
    13741 ttttaaattc tactaccatg tacacttacc ccctataaat tttgaaagtg gtttaaccct
    13801 ttgatttaat gatataacat catttaaact cattttacta tgattaaggt tagttttgca
    13861 atcgctttca tttttatgtt ttatcactta ttctcaagta ttttgaaatt gattggttac
    13921 tttttaaaat ttatatgggt cgcaactgct actttatcgt ttcgtcattt aatgtttcgg
    13981 atggtaggtc attatcaatt ttacgaacga ctttacaagg gtttccaacc gctaagctgt
    14041 gtggcggaat atctttagtg acaacactac cagcaccaat cacactgcct tctccaatcg
    14101 tcacccctgg taacacggct acatgaccgc caaaccaagt attactgcca atatgaatgg
    14161 gtccggcttt ttcaaaacct tcatttctat gatggaaatt aagtggatgt gtcgctgtgt
    14221 agaatccaca attaggtcct ataaaaacat tatcgccaat tgtaatctgt ccaccatcca
    14281 taaagtaaca attggtgttt acatagacat tttttcctag tttaacgttc caaccataat
    14341 ctgtatcaaa aggaatcgaa atacttacat tgtctgttgt tgtttgaaat aattgatcaa
    14401 ttaattcctt tcttttattt gtagcactcg gtcttgtatg atttaattca aagcaaatat
    14461 ctttcgctcg tgcacgttca ttgattaagt attgatcaaa gtttgcatcg taccattttt
    14521 ctgctaacat tttttctttt tcagtcatta cacctttcaa ctcctaataa cttatttact
    14581 tgtttaaaag ttaatcaaat aaaccttcgc ctatgcaact aatacgctat aacattatga
    14641 aatcatgacc ttatcaccct tatctataca attctcgcat caaatactgc taaagtagta
    14701 gataaattca atactacaga cgcattcatt ttttaatcta ttaacgtaca atgtgagtaa
    14761 gagaaatata aaggagtatg atagcgatga gaatattaat tacaggcaca gttgctatct
    14821 taatcattct aggtttggtc aaaacgatac aagattacga aatgacaaac gacacgagtc
    14881 gtcagttgtc agacaacaaa gatgatgata aagtcatcca tcttaataat tttaaaaatt
    14941 tacatgcgaa agaatttaac ccatctgatt tcttttaagt cacctaagaa ttgcaaatcc
    15001 agaagtcatt taagttttac ctttcattca tacatccttt aatattaatt acgacttctt
    15061 ttatatagat gctaagtaga gagattgttg tgcaatgttt gcacggcaat ctctcttttt
    15121 ctttttaaaa ttggtaaaag taaaacgcaa cgattgactt atatacctat agggggtaca
    15181 ttagacgtgt aacaatgaat cacagggagg caataatgtg gctaatacga aaaaaacaac
    15241 attagatatc actggtatga cttgtgccgc atgttcaaat cgtatcgaaa agaaactgaa
    15301 taaacttgat gacgttaatg cccaagtgaa tttaactaca gagaaagcaa ctgttgagta
    15361 taaccctgat caacatgatg tccaagaatt tattaatacg attcaacatt taggttacgg
    15421 tgtcgctgta gaaactgtcg aattagacat tacaggtatg acttgtgctg catgctcaag
    15481 ccgtattgaa aaagtgttaa ataaaatgga cggcgttcaa aatgcaacgg tcaatttaac
    15541 aacagagcaa gctaaagttg actattatcc tgaagaaaca gatgctgata aacttgtcac
    15601 tcgcattcaa aaattaggtt atgacgcgtc tattaaagat aacaataaag atcaaacgtc
    15661 acgcaaagct gaagcgctac aacataaatt gattaagctt atcatatcag cagtattatc
    15721 tttaccacta ttaatgttaa tgtttgtaca tcttttcaat atgcatatac cagcactatt
    15781 tacgaatcca tggttccaat ttattttagc tacacctgta caatttatta ttggatggca
    15841 attttatgta ggtgcttata aaaacttaag aaatggtggc gccaatatgg atgtacttgt
    15901 tgctgttggt acaagtgcag catattttta cagtatttat gaaatggttc gttggctaaa
    15961 tggctcaaca acgcaaccgc atttatactt tgaaacaagc gccgtactaa ttaccttaat
    16021 cttattcggt aagtatttag aagctagagc gaagtctcaa acaaccaatg cgcttggcga
    16081 attattaagt ttacaagcta aagaagcacg cattttaaaa gatggtaatg aagtgatgat
    16141 tcctctaaat gaagtacatg ttggagatac acttatcgtt aaaccaggtg aaaagatacc
    16201 tgttgatggc aaaattatta aaggtatgac tgccatcgac gaatctatgt taacaggtga
    16261 atctatccct gttgagaaga atgttgatga tactgtaatt ggttcaacga tgaacaaaaa
    16321 cggtactatt actatgacag caacaaaagt tggcggggac actgcgttgg caaatattat
    16381 taaagttgtc gaagaagctc aaagttctaa agcgccgatt caacgattgg cagatattat
    16441 ttctggttat ttcgttccta tcgttgttgg tatcgcacta ttaacattta tcgtgtggat
    16501 tactttagtt acaccaggta catttgaacc tgcacttgtt gcgagtattt ccgttctcgt
    16561 cattgcttgt ccatgcgcat tgggacttgc tacaccaact tctattatgg taggtactgg
    16621 tcgcgctgct gaaaatggta ttttatttaa aggtggcgag tttgttgaac gcacacatca
    16681 aattgatacc atcgttttag ataagacggg taccattaca aatggtcgtc cagtcgtgac
    16741 agattatcat ggtgacaatc aaacgctaca actacttgct actgctgaaa aagattctga
    16801 acacccattg gcagaagcca ttgtcaatta tgcaaaagaa aagcaattaa tattaactga
    16861 gacaacaaca tttaaagcag tacctggcca tggtattgaa gcaacgattg atcatcacta
    16921 tatattggtt ggtaaccgta aattaatggc tgacaatgat attagcttgc ctaagcatat
    16981 ttctgatgat ttaacacatt atgaacgaga tggtaaaact gctatgctca ttgctgttaa
    17041 ttattcatta actggtatca tcgcagtggc agatactgtc aaagatcatg ccaaagatgc
    17101 tataaaacaa ttgcatgata tgggcattga agttgccatg ttaactggcg ataataaaaa
    17161 cactgctcaa gccattgcaa aacaagtagg catagatact gttattgcag atattttacc
    17221 agaagaaaaa gctgcacaaa ttgcgaaact acagcaacaa ggtaagaagg ttgcgatggt
    17281 tggtgacggt gtaaatgatg cacctgcatt agttaaagct gatatcggta tcgccattgg
    17341 tacaggtaca gaagttgcca ttgaagcagc tgatattact attcttggtg gcgacttgat
    17401 gcttattcct aaagccattt atgcaagtaa agcaaccatt cgtaatattc gtcaaaatct
    17461 attttgggca ttcggctata atattgccgg tatccctata gctgcattgg gcttacttgc
    17521 gccatgggtt gctggtgctg caatggcact aagttcagta agtgttgtca caaacgcact
    17581 tagattgaaa aagatgcgat tagaaccacg ccgtaaagat gcctagattc cttaataatg
    17641 aaggattcgt tggtgattct gagataggct agtgattggc tctataatgt c
//
LOCUS       DANRDI010000042        17444 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.042, whole genome shotgun sequence.
ACCESSION   DANRDI010000042 DANRDI010000000
VERSION     DANRDI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.042"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(429..1712)
                     /locus_tag="PZ377_001119"
     CDS             complement(429..1712)
                     /locus_tag="PZ377_001119"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDM6358000.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(1990..3390)
                     /gene="dnaB"
                     /locus_tag="PZ377_001120"
     CDS             complement(1990..3390)
                     /gene="dnaB"
                     /locus_tag="PZ377_001120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDM6358001.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(3422..3868)
                     /gene="rplI"
                     /locus_tag="PZ377_001121"
     CDS             complement(3422..3868)
                     /gene="rplI"
                     /locus_tag="PZ377_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDM6358002.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(3865..5832)
                     /gene="gdpP"
                     /locus_tag="PZ377_001122"
     CDS             complement(3865..5832)
                     /gene="gdpP"
                     /locus_tag="PZ377_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDM6358003.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(5847..6785)
                     /locus_tag="PZ377_001123"
     CDS             complement(5847..6785)
                     /locus_tag="PZ377_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDM6358004.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7076..8044)
                     /locus_tag="PZ377_001124"
     CDS             complement(7076..8044)
                     /locus_tag="PZ377_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDM6358005.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8104..8202)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8407..8736)
                     /locus_tag="PZ377_001125"
     CDS             complement(8407..8736)
                     /locus_tag="PZ377_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDM6358006.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(8733..9428)
                     /locus_tag="PZ377_001126"
     CDS             complement(8733..9428)
                     /locus_tag="PZ377_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDM6358007.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            complement(10078..11364)
                     /gene="serS"
                     /locus_tag="PZ377_001127"
     CDS             complement(10078..11364)
                     /gene="serS"
                     /locus_tag="PZ377_001127"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDM6358008.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(11454..11670)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11743..13257)
                     /gene="hutH"
                     /locus_tag="PZ377_001128"
     CDS             complement(11743..13257)
                     /gene="hutH"
                     /locus_tag="PZ377_001128"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDM6358009.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     gene            13583..14395
                     /locus_tag="PZ377_001129"
     CDS             13583..14395
                     /locus_tag="PZ377_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDM6358010.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            complement(14482..17145)
                     /gene="gyrA"
                     /locus_tag="PZ377_001130"
     CDS             complement(14482..17145)
                     /gene="gyrA"
                     /locus_tag="PZ377_001130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDM6358011.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(17182..>17444)
                     /locus_tag="PZ377_001131"
     CDS             complement(17182..>17444)
                     /locus_tag="PZ377_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDM6358012.1"
                     /translation="DRELDKLKSELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRA
                     LLQVKLEDAIEADQTFEMLMGDVVENRRQFIEDNAVYANLDF"
BASE COUNT         5338 a         3458 c         2643 g         6005 t
ORIGIN      
        1 ttcttaagca caagatagat tatagcacag ccaatcttgc catgacaaga tattttttac
       61 ataattttgc tattattttt tgaagaatgt tataagtgaa aaattgcatt ttattggcag
      121 tctattcatt ataataatta cgctattttt gaaatttttc tattttaggt gctttcattt
      181 acacttacgt ctactctatt ttaatagcta ttttcgacat cagacttctt acattcaagt
      241 atttcatctt aattttcgtt aaatatcatc taatcttcta aattatgact agcaaatatc
      301 gcctttacgt tagtattttt tatctaaata atcgaatcga tacagaagca agcataacaa
      361 aagcctccta ccaataaaga tagaaggctc atgtatttat aatcattgtg tatgacttat
      421 ataaagttct accacaattc ttttaatagg tttgtttgtt ctctatctgg accaactgag
      481 aagatagaaa tttgtacatt acataattct gaaatacgct ctaaatattt acgtgcattt
      541 tcaggtaatt cttctaaagt acgcacattt gttacgtctt ctgtccaacc tggtaactct
      601 tcaaagattg gtttacaacg ttttaattga tctaagtttg ctgggtactc agtaatttct
      661 ttaccgtcta attcataagc tgtacagatt ttcactgtgt ctaggcctgt taaaacatcg
      721 attgagttaa tagataaatc tgtaatacca cttacacgac gagagtgacg taatacaact
      781 gaatcaaacc aacctacacg acgtggacgt cctgttgttg taccgtattc acgaccaacc
      841 tctctaatat gatgtccatc ttcatcgaat aattcagtag ggaatggacc atcaccaaca
      901 cgtgatgtat aagctttaca tacaccaatt acctttgaaa cgaatgtagg acctacacct
      961 gtaccaacag taacgttacc tgcaattgga ttacttgatg taacgaatgg atatgtacca
     1021 tggtcgatat ctaacattac accttgcgca ccttcgaaaa gtaccttttc atctgctaca
     1081 aatgcatcgt ctaagatttt tgatgtgtct gttacaaatt cttttagacg ttgacctgct
     1141 gcataatatt cttcaaagat atcatcaaat gatggacatg tttcgttaaa catacctttg
     1201 aaatatgctt gtttatattc aatgtttgat tttaataatc tttcgaatgt ttctttttca
     1261 agtaaatctg ccatacgaat accgatacgt tgaactttgt ctacatatgc tggaccgata
     1321 ccttttttag ttgtaccaat cttattgtca ccacgtaaac gttcttcata ttcatcttgt
     1381 gctaagtgat atggtaaaat cacttgcgca cgattagata tacgtaaatt acttgtagga
     1441 atgccacgtt catttaatcc gtctaattct ttcaatagtg caactggatc aacaacgact
     1501 ccgttaccga ttaccgctaa tttgtctttg taaaagatac cagatggtac taaatgtaat
     1561 ttatatgttt ctccgccaaa ttgaatggta tggcctgcat tattaccacc tgaaaaacgc
     1621 gcgataacat ctgactgttc tgccaagaaa tccgttattt ttccttttcc ttcgtctccc
     1681 cattgtgtcc caactactac gattgatgac atgtgagcac ctccaagttt ttctcattta
     1741 accatttgat attctaccaa tatcgagacc ataatgcaaa taaaaatcga acattgattt
     1801 tcggcctttt taaaagaaaa atcattttaa caaatcgttt tcaacgtcct aaaaaccctt
     1861 taacagaggc gttgacagta ctaatttatc actttcaata cacaaactta acatatatca
     1921 acccgttcag aacaaaataa aaaccgtaca attttatcat cttaatgatt attgtacgga
     1981 aaaacttttt tacatcatat ctgcatgtgc ataatcgata tcggtaaatt tattatattg
     2041 tttcataaaa tgtaacttaa ctgtgcctgt tggaccgtta cgttgcttag caatgataat
     2101 ttcaatttca ccgttttcat cattcgtttg tggctcgaaa ccaccatcat cgtcatcatc
     2161 ttcatcgccg ccacggttat agtaatcatc acggtataag aatgcaacga tatcggcatc
     2221 ttgctcaatc gaaccagatt cacgaatatc actcatcatt ggacgtttat cttgtcgttg
     2281 ttcaacacca cgagataact gacttaatgc gataactgga cattctaatt cacgggctaa
     2341 tgcttttaat gtacgagaga tttcagaaac ttcctgttgt ctgttatcgg acgcacgtga
     2401 accactacct tgaatcaact gtaagtagtc aatcacaatc atgtctaagc catgttcttg
     2461 ctttaatcga cgacatttag aacgtaaatc attaattcga atacccggtg tatcatcaat
     2521 aaaaatcttc gtacgtgata atttacctac cgctatagta aaacgactcc aatcttcctc
     2581 agtcatagta cccgttctta agcggtttga gtcaacattt ccagaactac aaatcatacg
     2641 tgtggctaac tgatcagcac ccatctctag cgagaaaata ccaactgtat acatatcttc
     2701 atgcgttgca actttttgtg caatattaag tgcgaacgcg gtcttaccta cagatggacg
     2761 cgctgcaagg ataattaaat catttcggtt gaaccctgct gtcatttggt ctaaatctcg
     2821 atatcctgta ggtatacctg gtgtttgacc actattttga tcaagctctt cagctgtttc
     2881 atacacttgt cctaagacgt ctcgaatgtc tttaaagcca tcgctttcac gagaagatga
     2941 tagctctaaa attcgacgtt ctgcatcact taaaatcgca tctagttcaa gttcatcatt
     3001 atatccatca ttggcaatac tatctgcagt ttgaatcaat ctacgtttta atgcatgctt
     3061 agaaacgata tcagtataat actgaacatt tcgcgtcgtt ggtacatttg tagataactc
     3121 tgcaagatat tgcgggccac ccgcttcatt caacgtacct tccgtcgata attgatccat
     3181 caatgttaca acatcaattt ctttattatc ttcatttaag tgcatcattg cacggaaaat
     3241 atgttgatgg gcacccctat aaaacgactc aggaagcaaa acttcctgag tagtattaat
     3301 caattctgga tctataataa ttgaacctaa gacagactgt tcagcttcat tgttatgcgg
     3361 catttgattt tgctcataca ttctatccat gaatggttac acctcttatt tcaatccaac
     3421 tttattgttc aactgtgtgt acgcgaattg taccttcaac ttctttatct aatttaacag
     3481 gtacattcgt atatcctagg gaatgaattc catttggtaa atccatttta cgtttatcaa
     3541 ttttaatatc atgttgtgct tttagtgctt cggcaatttg ttttgtactt actgacccaa
     3601 acaatttacc accttcacca gtttttgctg atacttcaac ttcaatgttt gataacgttt
     3661 cttttaatgc tttagcatct tcaatttctt gttggcgttc ttgttttgca cgttttttct
     3721 gtaactctaa ttgtttaagg ttacctggtg ttgcttctac agcataattc tttttcaata
     3781 agaagttatt tgcataacct actggtactt ctttaacttc acctttttta cctttacctt
     3841 taacatcttg tgtaaaaatt actttcatgc atcttcactc ctacttaatt gttctgtaat
     3901 tgcttgttgt aattgtgcta tcgcctcttc gactgtcaca cctttaagtt gtgttgccgc
     3961 attggttaaa tgtccaccgc caccaagtgc ttccattgtt aactggacat ttactgaacc
     4021 gagtgaacgc gcagatatac caatcagatt atcttcacgt ctcgcaacaa catatgatgc
     4081 ttcaatacct tctaaactta acagttcatc tgctgcttgt gcaactgtta ctggatgata
     4141 aattttatcg tctgaaccat gcgcaatggc tatgccatta tcttcaactt ttacagttcg
     4201 aattaattca gatcgattaa tgtaagtatc cacatcatct tttaagaaat gttgcgttaa
     4261 aatcgtatct gcaccatgtg cacgtaaata actcgctgca tcgaatgttc ttgatcctgt
     4321 tcgtaatgta aagtttcttg tatctacaat aatacctgca tacatcactg ttgattcaag
     4381 acgtgttaaa cgttgttctg ttggttgata ttccagtaac tctgttacca attcagctgt
     4441 cgaacttgcg tatggttcca tatatatcaa caatggatta gagatgaagc tttcaccacg
     4501 tctatgatga tcgataacaa ctttacggtt tgctttattt aagacatttt catctaaaac
     4561 cagttccggt ttatgcgtat caacaatcac tacggttgtc ttagatgtca tcatatccca
     4621 agcatcatct gatgtaataa atcgctctct taactctggc tttttatcta tttcgttcat
     4681 cacgcgtcgt aatgttggat caatgtcagt ctcatttaat acgatgtatg cttctaaatt
     4741 attcatcatt gcaaatctag acacaccgat tgctgcacca attgcatcta agtcaggacg
     4801 tttatgtccc atgataatga ctttgtcacc ctctgcaagg atatctttta acgcatgtga
     4861 gatcacacgt gctcttacac gagtacgttt ctccatcggg tcagtcttac cgccatagaa
     4921 acgcacatta ccattaatac ttttaattgc aacttggtcg ccaccgcgtc ctaatgctaa
     4981 gtctaggcct gattgtgata attcacctaa gtcgattaaa ttttcagtac cttcaccaac
     5041 accgatactt aatgttaatt gggcacgata accaacactt ttttcacgta attgactcaa
     5101 gatatcaaat ttagattctt ctaagtcagc taatattttt tgatttaaat aggctacgaa
     5161 ttgatcggaa ctgtatcttt tgaaaaatat attatactca gttgcccatc gactaatgac
     5221 acgcgttacc attgagttga tttccgaacg ctgcgtatca ttcatatttt gcgtaatctc
     5281 atcgtagtta tctaaaaata atgtcgcaat gattggttta gaattttcat atagttcatt
     5341 tgtttgtact tgttcagtta tatcaaagaa atagaggcag tgatcattct cagaataacg
     5401 tacttggaaa tgatactgat tatattctat ttcaacggat ttcactctat ctaattgctt
     5461 taaaatgttt ggaaatactt catttacaga ttcagaaatg acattcgctt ccatatgatc
     5521 tgtcataaat tggttaaccc attcgatgtg atcattttca tctaaaacaa tgataccaat
     5581 tggtaaatgt ttgattgctt tattatttgt tgttgaaatt tgagcactca aaccatctac
     5641 ataactatcc attttcatta aagcttgtct gaataaaatg atgctaacaa taatcatcac
     5701 gacaagaacg atagatgcaa ttagtgctat aagactatta aagataaacc atacacccat
     5761 taaaacaatt gctgtgatga tcatgatgac aaatggtatt agtaaagctt tcttagtgga
     5821 ctgccgattc attattccac ctctattcac tttttagaat tatttttcat gattcgcttc
     5881 aaattcaaac ttaaatcgat aacaccaagt agtcctacaa tatgtgtcgt aggtgtcagt
     5941 attgtaccga taaccaatag taaaatcgtt actgcattcg gcaaaccttt cgctttacca
     6001 aagaaatgaa taacacttaa accttgaata tacattacta atgataacac aagttggaag
     6061 tttaaaagaa tgctctggaa cacactcggt tgacctgtaa ataataaaca tatgataaca
     6121 ataatgtata tccataataa aataccgctc atttgccacg cgaaaagtgg cttaaataca
     6181 ggtgtagcga ttttaaattt tcgtaaaatc ggaaatgtaa cgattaagtt aattaagacg
     6241 attaaaaatg taatgataat gatgaaacct ggtaattgaa cggtcgcttg tctaaaccct
     6301 tcttctaata tttgggtcat attcgcatcg gcaccgctca tcgtaatcgc ttcatgtaat
     6361 gtttgcttga aaggttttac tatgctcgct gatggtggaa tccttccgaa tgtttgtagt
     6421 aacataaaag cgattaatga aattaagctc atcgctactg ttgttacgta taatattctt
     6481 tctttagacg ttctttcttt gagcaattga ccaataatta aacttgcaat taagactaat
     6541 atgatggcac ttaaaacgaa agtattacct aaaacagttg ttataattac tgtaataagt
     6601 gcactaatcc cgaaagattg tattgattta ttccataaaa cgatacctgg tatggttgca
     6661 aataatgcaa atattagtcc taaaccaggc actagatata aagctaaagc gacaaatacc
     6721 aacgcaatcg ttgcaattat tgttgcttta ggttgtattt ttgaaaacac ataagccact
     6781 cccatatttt taactatagc tattatttta acctctttaa tgaaaattaa caatttatag
     6841 attgtatgct tctatttcat ttaattgaat aataactttc atgttttata agtaattaac
     6901 atactcattt gaatcgcttt tgtgtgcttt cattttcaac atgattattt aatcccacta
     6961 catagcaatc aagcttgatt tagatttaca atacatttcc actctcatgt actctagatg
     7021 tttttgaata tgataactgt gatttagtgg cttcattctt tgaaaatata tattattact
     7081 tacgcttaaa atgctttaaa tttaagaaat gatataagtt aggtgcccag gtactaaagt
     7141 ttagtaggaa tccatcatgc ccaacattat caggcacgaa gaaatgacga tgatatttaa
     7201 aacgttcacc taatgcacga acttgatcat ccggatatag caaatcatct atgaacccca
     7261 tcgttaacac ttttgtttct aaatttttaa aaacatgcgt tacgtctgtg cgacctcggt
     7321 caatgttgtg actatccaat acatctagca gtgtcagata acaattcaaa tcaaaatgtt
     7381 ctttaaattt attaccttga tgttgttggt atgcgactac ttcatccggc gtgaaacgtt
     7441 catcataact ttttgatgat cgatatgtca aaaaacctaa ttggcgtgca atacttagac
     7501 cttccttacc accaagatga atggcttgcc ttgcaatttc attgaaagct ctactataag
     7561 atgatgttcg acttgttgca gcaaggataa tggctttatc tacttcaaac tgttgattgt
     7621 agagtagttc cattgcttgc atacctccaa gacttccccc tattaaaata ttaatcttat
     7681 cataaccaag ggcttgtata cctcgttcat tcgctctgac tatatctctt aatgttaatt
     7741 ttttaggaaa atgagggtcg tttaaaggtg aacttgaacc gaaaggacta ccaataacat
     7801 caaatgttaa aaattgataa tcgtgaatgg gtatatatcc cccatcaata atttctcgcc
     7861 accaacccgg ataatcatct gttccatatg ttaaatgatt gccagttaat gcatgacaaa
     7921 ctacaactaa tggttgtcca tgataaccga catgctcata tctcaaacgc aagttatcta
     7981 tgacttcccc agattctgta ataaattccc ctaaatttaa agtatctact gtgtaatttg
     8041 tcattgttct ttcctcctta aacaaaaaaa cttctcaccc tattgaaaag taagaagtct
     8101 ttatacttat cattcgagta actcgttggt tttagcaccg tgctataaag tcggttgctg
     8161 aagtatcaca gggccaagtc cctcaacttc tcacaataag aaatatgaaa ttgttatgtg
     8221 ttagttgaga ttcagtgatg aattactttt atcatttaaa atgttgttat cattgtcatg
     8281 cgttagccaa atcgcttacg tatacacgat tcccaatctt aacatagacg atttgtatat
     8341 cagaattttc tgattactaa cagtttacct aagtttaaat atctgttcaa tgattttcag
     8401 ttatttttaa aagaaaaatc gtaatgctgc catgataaca atcccactaa taattgtaat
     8461 agttaaacta cgcgtgatta tagataaaat aaccgtcgga atgagcgcga taatgtaagg
     8521 gatgtttaat gtatacccct caccatgagg cgtctgttga ataatgctgt caatgacaag
     8581 tgccgtaaat agtgtgattg ggataaatga tagccatcga accacgacat caggcaattg
     8641 cacttttgaa atcatgataa aaggtataat tcgaattaat agcgttacga taccacacaa
     8701 taaaataagt attaacatgt tcatatgagt tatcattgtt ccatcatcac tcctaacgct
     8761 gctgaaattg tggctgcaat taatattgct agatatgaag gcataaacat acttagcgat
     8821 aacatcatta ctatgacggc aataatgagt actatgtaaa ttcttaatcg cgatttagta
     8881 attgattcaa attgcgcaat ggccaaaaag ataaacatag ccgtgatagc aaaatctaac
     8941 cctagcgttt gcggatttga gatatattcg ccaaataaag ccccagctac acatgaaatt
     9001 gcccaaaata aatatgctgt gatgttaaga ccatgcatcc aacgatcatt gatagcttct
     9061 ccttttaaat aaggtgtaat ggcgacgcca aacgtttcgt cagttactaa tgaacctaat
     9121 ccaacacggt tccaaaaccc atatgtcttg aagtttggtg caagcgacat acttaaaagg
     9181 aacattcttg aatttacgat aaatacagtt agtacaatcg ctgatatagg tgtacctgct
     9241 ataaacaacg cgcacataat aaattgcgca gcaccggcat atataacaag acataacaag
     9301 acaatttcta aaatactaaa gttttgagac gaagccacaa taccaaatga aataccaaca
     9361 ccggcataac ccaataatgt tgggatacac tcttgcacgc cttgtctaaa acttaaatgt
     9421 gttgtcatct caattacctc ctttgccact ttatatttat ataattacga aaaaataata
     9481 tacctagtct aatacagttc tataccctaa gcaataacat tagacatcag tttgtctgag
     9541 gttagacata ccggagtctt tagtcagctt catattaact ttttattttt gagaattttc
     9601 aattttttat ttaagactac ctccatattt tctatggatt tgtagttgtt tttaagtatc
     9661 aattttataa atttttatat ctgatgatga gtctgggata ttgattcatg taccactccc
     9721 ttataatcat cccctccccc taccctactc catcgatata actcatacta catatcaacg
     9781 aaatcagtat tttatcgctt cctttcctat attggtgatg ctcaaacttg ttacgtttta
     9841 gattgtttta gttcatcata attatcccgt attgttgcta taatgaaatg cgttcacccc
     9901 attaaaccac aaacttaatt tattgttgtt atgtgcattg gctcactatt atacttttac
     9961 agcacaaaaa aagtggcgac agcttcgtca ccacttttta aaatattatt taaagtatct
    10021 tgcccttgct ttaagtatac gtagatatat actttttaaa gcttgtagct aaagccttta
    10081 tttaactggt tttgaaattt gtgttttacc acccataaat ggtactaatg cttctggaat
    10141 tgttactgtt ccatcttcat tttggtaatt ttcaacaata gcagcaaatg tacgtccaac
    10201 tgctaaacca ctaccattta atgtatgtgc taattctggt ttagctgctt tgtcacgctt
    10261 gaagcggatg ttagcacgac gcgcttggaa atccgtacag tttgagcatg aactaatttc
    10321 tttataatca ttgtagcttg gtaaccaaac ttctaaatca tatgttttgc ttgcactaaa
    10381 tccaatatca cctgtacata aaataacacg acggtatggt aaacctaact cttctagaat
    10441 tgcttctgcg tttgttgtca tttcttctaa agcattccat gaatcttcag gttgttcaaa
    10501 acgtaccatt tccactttat cgaattgatg taaacgaatt aatcctcttg tatctctacc
    10561 tgctgatcct gcttcactac ggaaacatgc agattgacca gtgaattttt caggaagtac
    10621 acctggttga ataatttcat tacggtagaa attcgttaat ggtacttcag cagttggaat
    10681 tgtatataat ccttcttttt ctactttaaa taaatcttct tcaaatttag gtaattgacc
    10741 tgtaccatac attgtatctg cgttcacaag ctgtggtacc atcatttctg tataaccatg
    10801 ttgtgttgta tgttttgtaa tcatatagtt cattaaagca cgctctaatt gcgcaccttc
    10861 atttgttaaa tatacaaaac gcgcacctga aacttttgct gcacgatcaa aatcagccat
    10921 tttcaattct tctacaatat cccaatgtgc tttgggttca aatgaaaact cacgtggtgt
    10981 accccacttt ttaacttcaa cgttatcttc atcagattca ccttgaggta catcatcact
    11041 tattaaattt ggaatacgac aaaggatacc tgtcatttta ttatcaattt catttaattg
    11101 actatctttt tctttaatat cgtcacctaa tgtgcgcatt tcagcaatca catcatcagc
    11161 attttcttta ttacgttttt ttaatgcgat ttcttcgctt actttattac gacgtgcttt
    11221 catttcttct gttgcactaa ttaatttacg tcgttgctca tccaattcta aaatttcatc
    11281 tacaactttt ggatcatctc cacgtaattc aattttgctc ttaactgtgt caggctcatt
    11341 tctgaataat ctaatgtcta acattaacct tcatcctttc ccaaataatt atcatttatt
    11401 atggaatgac gtacgtcttt attttttaga aaataaaaaa agaccacatc cctacaaggg
    11461 acgtggtcta cgcgttgcca ccctatttaa caatttaagt tataaagata cactaaacct
    11521 aaattgcact tcactaaaat aacggttatc accgattgtt cttttaaatt aagtaggtag
    11581 attcatatat atgttgattc ttgttcacac taaccacaag ctctctgata tcgaacacta
    11641 tatattactt gtcctacgaa caatgtctta ttaagttatt tttaatatag caaactatat
    11701 ttgctttttc aagtaacgat ttcaaacatc actcatgtcg atttagtgac atgcagtcgt
    11761 ttgataaatt gattgcttta aatactgtgc aaccgcttca atatctttat gaaattgacg
    11821 atcatgtgta atggatggca cgatacttcg aaactcatca tacttgcgac gtgtttttgg
    11881 tgataatcct tcaacacctt ttaactctgc tgcttgtaat gcaataacac attcgattgc
    11941 cagcacacgt cttgcatttt caataatttg ataaccatgt ctagcagctg tagttcccat
    12001 agatacgtga tcttcttggt tcgcagatga agtgatagaa tcaacactcg ctggatgcgc
    12061 taaagtttta ttttcagaaa cgagacttgc agcagcatat tgcataatca tcgcgccact
    12121 ttgcaatcct ggctctggac taagaaatgc tggtaaatca ccatttaatt gaggatttac
    12181 tagtcgctct agacgacgtt ccgatacgtt tgctaattca cttacaccta atttaagatg
    12241 atctaatgca aaagcaatag gttgtccatg gaagttacca cctgaaataa caaacgtttc
    12301 atttgcttcc tcaaatataa gtggattatc attagccgca ttcatttcaa attctaattg
    12361 ctgtttaaca taattgaata cttgaaaact cgcgccatgg atttgtggta tacaacgcaa
    12421 cgtatatgca tcttgtacac gtatttctga ttgtcgcgtc gttaatgttg atccttctaa
    12481 ccaatcacgc atacgcgctg ccacattaat ctgttcttga aaattacgaa ctgcgtgcac
    12541 atcatgtcga tatgcatcta taatgccatt aagagactga tgcgttaatg cagcaatcca
    12601 ttcagattgg taacctaaat cttctgcttc tatataacta atgacacctt gagctgtcat
    12661 agcttgcgta ccattaatca atgctaaacc ttctttagcc tgaaggttca aaggttgtct
    12721 atttaattct cttaatacat cgtcactatc cttttcttcc cctctgtaca atactttccc
    12781 ttcaccaatt aatgctaatg ctaaatgtga taatggcgct aaatctcctg atgcaccgag
    12841 agagccttgc tgtgggatta tcggtataat acgttcattt ataaaaaatt gtaattgtct
    12901 cactaattct aaagtggcac ctgaatgacc ttttaataat gtattcaatc gtaaaatcat
    12961 catgactaat gctacttctt ttgaaaatgg ctcacctagt ccacaggcat gtgagcgtat
    13021 cagattcact tgtaattcat tatattgcgt cgggtctata cgtacatcac taaataaccc
    13081 aaaacctgta gtaataccgt aaaccgtttc ctcattttca ataatacgtt caactaccgc
    13141 tctacttttt ttgacacgtt ctaacgcatc atcaataatt tcaatctttg attgttgttg
    13201 taaaaatgat ttaatatcct caattgttag tgtttcacca tctaaatata aagtcatata
    13261 tgttaccccc ttgtttatat taagtaaccc atccttcttg aagtatacgt tttcattttt
    13321 attgaaacaa tggttttacg tacatttata acctattatc agagcactat tgtagtgcgt
    13381 taaaggatat taagattgtt gtaagcatat ttaataattt atctattgac gaattgcata
    13441 tacaggtata gtattttcta ttgtatttaa cgacaaataa taatgaattc agaaatttat
    13501 aatacatttt gttaaaagtt actatatatt tttaaaattg aataaattcg gaaaaggctt
    13561 ttacatggga ggttatatca ctatggaaac gttaaattct attaacattc ctaagcgtaa
    13621 agaagattca cataaaggtg attatggcaa aattttatta attggtggat ctgctaactt
    13681 aggtggtgcc attatgttag cggctcgtgc atgtgtattt agcggtagtg gtttaatcac
    13741 tgtagctaca catccaacaa atcattcagc attacattct cgttgcccag aagcgatggt
    13801 tattgatatt aatgatacga aaatgttgac gaaaatgatt gaaatgactg acagtatact
    13861 aattggtcca ggtcttggcg ttgatttcaa aggaaataat gccattacat tcctactaca
    13921 aaatatacaa ccgcatcaaa atttaatcgt agacggcgat gcgattacaa tctttagtaa
    13981 actgaaaccg caattaccta catgtcgtgt gatctttaca ccacacctca aagaatggga
    14041 acgattaagt ggtattccta ttgaggaaca gacatatgag cgtaatcgtg aagcagttga
    14101 tcgtttaggt gcaactgttg tacttaaaaa acatggtact gaaattttct ttaaagatga
    14161 agactttaaa ttgacaatcg gtagcccagc aatggcgact ggtggtatgg gcgatacact
    14221 tgctggtatg attacaagct ttgtcggtca atttgataac ttaaaagaag cggttatgag
    14281 tgccacatat acacatagtt ttattggcga aaaccttgca aaagatatgt atgtggtgcc
    14341 accatcaaga cttatcaatg aaatacctta cgcaatgaaa caattagaaa gttagtcatt
    14401 actaatcatt gaatatagta aagcattact ttctagcata aaaataagac tcccctacat
    14461 atagggaagt cttatttttt attattcttc atctgatgat tgttgtatat cttcttcaac
    14521 acgatccatg aaatcttgtc ttacttcaat acgtccatct tcatcatttt cttctgaatc
    14581 aatcacttca gtatgaattg catttcctgg tgtttcatca tttacaaccg cttcacgttg
    14641 ttgttcagta ccatcttcag atacagttga agtagattgc tcatcttcat tcgtttcatc
    14701 ttctgcatct tcttttactt tagcaaccgt tgaaacaaat tgatcatcac ctaagcgaat
    14761 taagcgaaca ccttgtgctg cacgaccatt ttgagaaata tctgcaacat ctagtcgaat
    14821 aatgacacct gcattagtaa caatcattaa atcttcttca ccagttactg tagtgataca
    14881 tacaacatta ccattacgct cagtaatcgt agctgtttta atacctttac caccacgatt
    14941 tgataagcga tagtcattaa ctggcgtacg tttaccataa ccattttcag taactactaa
    15001 tacttcatca acactgtttg catgagctac atcaagccct acaacttcgt caccttcacg
    15061 aagtgtaata cctttcacac ccgttgctgt acggcctaaa ggacgtaatg ttgattcagg
    15121 gaatcgaatt aatgatgcat gtgatgtacc aatcaagata tcttcttgac cacttgttaa
    15181 gcgaactgca attaactcat catcttctct gaacgaaatc gcaatcttac catttctatt
    15241 tattcttgag aagttactta atgctgaacg tttaacgaca ccacgtttag ttgcaaacac
    15301 taagaagttg tcttcacttt caaggtcttt aacagcaatc attgtactaa tgacttcatc
    15361 attttcaagt tcaatagcat tcactacagg aataccttta gactgtcttg ataactcagg
    15421 cacttcgtaa cctttaagtt tgtatacacg acctttgtta gtaaagaaca atacatggtc
    15481 atgtgtactt aaagttacca attgactgac aaaatcttct tccaatgtat tcataccttg
    15541 aacaccacga ccaccacggt tttgagcacg atatgtagat accggcaaac gtttaatgta
    15601 gttattatgg cttagtgtaa ttactatttg ttcttctgga attaagtctt cgtcctctaa
    15661 gttttcaaat ccacctaatt gaatttctgt acgacgatca tcaccgaaac gatctctaat
    15721 ttcagtcaat tcatctctaa ctaactgtaa taacacttct tcatcagcta agattgcttc
    15781 taattcacta atataattta ataactcatt atattcagct tcaattttgt ctctctctaa
    15841 acctgttaga cgtcttaaac gcatgtctaa aatagcttga gcttgttttt cagaaagttt
    15901 gaagcgttgt tgcaagcttt ccattgcaac tttatctgta tctgactcac gaatcgttga
    15961 aataatttca tcgatatggt caagtgcgat acgtaatcct tctaaaatgt gggcacgatc
    16021 tttagcttta cgtaagttgt attgcgtacg tcttctaaca actgtctttt gatgctctaa
    16081 ataatgtacc aacgcttctt ttaaattaat aagcttcggt ctaccattta caagtgcaat
    16141 catattcaca ccaaatgatg tttgaagagg tgtttgtttg tataagttat ttaaaatgac
    16201 actagcattt gcatccttac gcacatcaat aacgacacgc acaccagtac gtaaacttgt
    16261 ttcatcacgt aaatcagtga taccgtcaat tttcttgtca cgaacgagct ctgcaatttt
    16321 ttcaatcata cgagccttat tcacttggaa aggaatttca gtgacaacaa tacgttgacg
    16381 tccgcctcca cgttcttcaa taactgcacg agaacgcatt tgaattgaac cacgacctgt
    16441 ttcatatgca cgtctaatac cactcttacc taaaataagt ccagcagttg ggaaatcagg
    16501 accttcaata tcctccatta actcagcaat tgaaatatca gggttcttac ttaagctaag
    16561 tacaccattg attaattctg ttaagttatg tggtggaata ttcgttgcca tacctaccgc
    16621 gatacctgat gcaccattgg ctaataagtt agggaatcga gcaggtaaga ctgacggctc
    16681 tctttcatta ccatcatagt tatcgataaa atctattgta tctttattaa tatcacgtaa
    16741 cagttcaagt gtgattttag tcatacgcgc ttcagtataa cgcattgctg ctgcgccatc
    16801 tccatccatt gaaccaaagt taccttggcc atcaacaagc ggataacgat aactgaaatc
    16861 ttgagccata cgtaccattg cttcataaat agataagtca ccatgagggt gatatttacc
    16921 cattacgtca ccaacgatac gtgctgattt tttatatgat ttatccggtg tcataccttg
    16981 ttcatttaat ccatatagta tacgacgatg tactggtttt aaaccgtcac gaacatctgg
    17041 caatgcacga gcaacgataa cactcatcgc ataatctaaa aatgattcac gcatttcact
    17101 ggtaatattt cgttcattta ttcttgattg aggtaattca gccatcaaga gttcctcctt
    17161 caaaagttca gttcacagcg cttagaagtc taagtttgca taaactgcat tatcttctat
    17221 aaattgtcta cggttttcta caacgtcacc cattaacatt tcaaatgttt ggtccgcttc
    17281 aatcgcatct tcaagtttta cttgtaaaag agcgcggtgc tcagggttca ttgttgtttc
    17341 ccataattga tctgcattca tttctccaag acctttgtat cgtgcaatag accattttgg
    17401 tgttggattc aattcagatt taagtttatc aagttcccta tcat
//
LOCUS       DANRDI010000043        17402 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.043, whole genome shotgun sequence.
ACCESSION   DANRDI010000043 DANRDI010000000
VERSION     DANRDI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.043"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..644
                     /locus_tag="PZ377_001132"
     CDS             <1..644
                     /locus_tag="PZ377_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDM6358013.1"
                     /translation="GINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHFPEMW
                     LLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMDAKVM
                     ASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKEMIQA
                     HQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            678..1010
                     /locus_tag="PZ377_001133"
     CDS             678..1010
                     /locus_tag="PZ377_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDM6358014.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            1011..1811
                     /locus_tag="PZ377_001134"
     CDS             1011..1811
                     /locus_tag="PZ377_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDM6358015.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            1801..2745
                     /locus_tag="PZ377_001135"
     CDS             1801..2745
                     /locus_tag="PZ377_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDM6358016.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            2723..3745
                     /locus_tag="PZ377_001136"
     CDS             2723..3745
                     /locus_tag="PZ377_001136"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDM6358017.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNEDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            4060..5085
                     /locus_tag="PZ377_001137"
     CDS             4060..5085
                     /locus_tag="PZ377_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDM6358018.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(5178..6524)
                     /locus_tag="PZ377_001138"
     CDS             complement(5178..6524)
                     /locus_tag="PZ377_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDM6358019.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(6539..6823)
                     /locus_tag="PZ377_001139"
     CDS             complement(6539..6823)
                     /locus_tag="PZ377_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDM6358020.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(6825..7268)
                     /locus_tag="PZ377_001140"
     CDS             complement(6825..7268)
                     /locus_tag="PZ377_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDM6358021.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(7273..9228)
                     /locus_tag="PZ377_001141"
     CDS             complement(7273..9228)
                     /locus_tag="PZ377_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDM6358022.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            9440..9859
                     /gene="mepR"
                     /locus_tag="PZ377_001142"
     CDS             9440..9859
                     /gene="mepR"
                     /locus_tag="PZ377_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDM6358023.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            9966..11321
                     /gene="mepA"
                     /locus_tag="PZ377_001143"
     CDS             9966..11321
                     /gene="mepA"
                     /locus_tag="PZ377_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDM6358024.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            11425..11865
                     /locus_tag="PZ377_001144"
     CDS             11425..11865
                     /locus_tag="PZ377_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDM6358025.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(11948..13306)
                     /gene="glpT"
                     /locus_tag="PZ377_001145"
     CDS             complement(11948..13306)
                     /gene="glpT"
                     /locus_tag="PZ377_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDM6358026.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            13614..14540
                     /locus_tag="PZ377_001146"
     CDS             13614..14540
                     /locus_tag="PZ377_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6358027.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            14554..15615
                     /locus_tag="PZ377_001147"
     CDS             14554..15615
                     /locus_tag="PZ377_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDM6358028.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            15629..16195
                     /locus_tag="PZ377_001148"
     CDS             15629..16195
                     /locus_tag="PZ377_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDM6358029.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(16255..17250)
                     /locus_tag="PZ377_001149"
     CDS             complement(16255..17250)
                     /locus_tag="PZ377_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDM6358030.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
BASE COUNT         5888 a         2674 c         3383 g         5457 t
ORIGIN      
        1 atggaataaa tcctacatat gatagttacg atgaatcgat ttcgttatta cgtgattatc
       61 ttacaataaa ggataaacca agtgcgcata cgttaggtgt ccaaccacac attgatcatt
      121 ttccagaaat gtggttatta agtagtagcg caacatctgc caaaatagct gccgaactag
      181 gtatagggct ttctgttgga acatttttgc taccagatat aaatgcgata catgcagcga
      241 aggataacat tgatatttac aaaaaacatt tccaagcatc aacgattaaa atggacgcaa
      301 aggtgatggc atctgtattt gtcattgtag ctgataacga agcggaagta gcagcattac
      361 aacatgcctt agatgtttgg ttattaggta aattacaatt tgcagaattt gaagattttc
      421 cttcagtaga cacagcacaa aagtataagc ttaatgatcg agacaaagag atgattcaag
      481 cacatcaagc acgcatcatt gcaggtacac aagaaaaggt taaagcacaa ttagatgatt
      541 tcattgctac gtttgaagtt gatgaggtgt tagtagcacc gcttattcca ggtattgaac
      601 agcgttgtaa aacattaaaa ttactcgcgg aaatttattt gtagcatttt aaatagaaga
      661 gaaaggatga agataagatg aaaaagttag ccaattattt atgggtagaa aaagtaggag
      721 atttgtatgt gtttagtatg acacctgaat tgcaagatga tattgggaca gtaggttatg
      781 ttgaattcgt aagtccagat gaagttaaag tggatgatga aattgtgagt atcgaagcat
      841 cgaaaacggt cattgatgtg caaacgccat tgtcaggaac gattattgag cgaaatacaa
      901 aagcggaaga agaaccgaca attttaaact ctgaaaaacc agaagaaaat tggttgttca
      961 aattggatga tgtcgataaa gaagcattcc tagcattacc ggaggcttaa atggaaacgt
     1021 taaaatcaaa taaagcgaga cttgaatatt taatcaatga tatgcatcga gagagaaatg
     1081 acaatgacgt attggtaatg ccatcttcat ttgaagattt gtgggaatta tatcgaggct
     1141 tagcaaatgt cagaccggca ttacctgtaa gtgatgaata tttagctgta caagatgcta
     1201 tgttaagtga tttgaatcgt caacatgtta cggatttgaa ggatttgaag ccgataaaag
     1261 gtgacaatat ctttgtttgg caaggtgata tcacgacgtt aaaaatcgat gctattgtta
     1321 atgctgcaaa tagtcgtttt ctaggatgta tgcaagctaa tcatgactgc attgataata
     1381 ttattcatac aaaagcgggt gttcaagttc gacttgattg tgcagagatc attcgacaac
     1441 aagggcgcaa tgaaggtgta ggtaaagcca aaataacacg tggatataat ttgtcagcaa
     1501 agtatataat tcatacggtt ggtccgcaaa tacgtcgatt gcctgtttca aagatgaatc
     1561 aggacttgtt agctaaatgt tatcttagct gtcttaaatt ggctgatcaa catagtttaa
     1621 atcatgtcgc tttttgctgt atatctacag gtgtatttgc ttttcctcaa gatgaagcag
     1681 cagaaattgc tgttcgaaca gtagaaagct atctcaaaga aacaaattca acattgaaag
     1741 tcgtgttcaa tgtatttaca gataaggatt tacaactgta taaggaggca tttaaccgtg
     1801 atgcagagta gtaagtggaa tgcaatgtct ctgttaatgg atgacaagac aaagcaggct
     1861 gaagtattgc gtactgcgat tgatgaagca gatgcgatag tgattggaat tggtgcaggc
     1921 atgtctgcat ctgacggatt tacatatgta ggagagcgtt ttacggaaaa tttcccagat
     1981 tttattgaaa aatatcgctt ctttgatatg ttgcaagcga gtttacatcc ttatggcagt
     2041 tggcaagagt attgggcatt tgagagtcgt tttattacat taaactattt agatcaacct
     2101 gtaggtcagt cttacctcgc tttaaaatcc ttggtggaag gtaaacagta ccacattata
     2161 actacgaatg cagataatgc tttcgatgta gctgattatg atatgactca tgtatttcat
     2221 atacaagggg agtatatact gcaacagtgt agtcagcatt gtcatgctca aacgtatcgc
     2281 aatgatgatt taattcgtaa aatggttgtt gcgcaacaag atatgcttat accttgggag
     2341 atgattccaa gatgtccaaa atgtgatgcc ccaatggaag tgaataaacg taaagcggaa
     2401 gttgggatgg ttgaagatgc tgaatttcat gcgcaactac atcgttataa tgcttttcta
     2461 gagcaacatc aagatgataa agtgttgtat ttggaaattg gaattggtta tactacacca
     2521 caatttgtga agcatccttt tcagcgtatg acacgtaaaa atgaaaatgc cctttatatg
     2581 acgatgaata aaaaggcata tcgcattccg aattcaattc aagaacgtac catacattta
     2641 actgaggata tctcaacatt gattacagca gcactccgga acgacagcac aacgaaaaat
     2701 aacaacattg gagagacaga agatgtactt aatagaaccg attagaaatg gagaatatat
     2761 tactgatggt gcgattgcac tcgctatgca agtttatgtt aaccagcata tctttttaga
     2821 tgaagatatt ttattccctt attattgtga tccaaaagtg gaaattggac gttttcaaaa
     2881 tactgctata gaagtgaatg aagattatat agataaacac agtattcaag tagttcgccg
     2941 agatactggt ggtggcgctg tgtatgttga taaaggtgcc gttaatatgt gttgtatttt
     3001 agaacaagac acttcaattt atggtgattt tcaacgattt tatcaaccag ctataaaggc
     3061 gttgcataca ttaggtgcaa cagatgtggt acaaagcggt agaaatgatt taacattgaa
     3121 tggtaaaaaa gtgtcaggcg ccgcaatgac attaatgaat aatcgtattt atggcggtta
     3181 ttcgctatta cttgatgtta attatgaagc aatggataaa gtgttaaagc ctaatcgcaa
     3241 aaagattgca tcgaaaggga ttaaatctgt gcgcgcacgt gttggtcatc ttagagaagc
     3301 actggatgaa aagtatcgtg atataaccat tgaagaattt aaaaatttaa tggtgacgca
     3361 gattttggga atcgatgaca ttaaagaggc gaaacgatat gaattaacgg atgcagattg
     3421 ggaagcgatt gatgaattag ctgataaaaa gtataaaaat tgggattgga attatggcaa
     3481 gtcacccaaa tatgaataca atcgaagtga aagattatct tcaggtacgg tagacataac
     3541 aatttctgtt gaacaaaatc gtatcgcaga ttgtcgtatt tatggggatt tctttggaca
     3601 aggtgatata aaagatgtgg aagaagcatt acaaggaaca aaaatgacaa gagaagattt
     3661 aacgcatcag ttaaagcaat tagacatcgt ttattatttt agcaatgtta cggtagaagc
     3721 attagtggat atgattttaa gttaatattg ttattttatg tatgctgaat cattggaagt
     3781 gtttgcttgc tcttgaaaag gtgacaatag tgtttggtga aggttgaaca tatgagtgga
     3841 aattattgcc tttaactatt caaagtatga tatatatatg gtttttgttt ctaaatgatt
     3901 gggtatttga aaatagatga gtttaatatt ttaaggaata taatgatgtt tacttttata
     3961 attcatatag aatattaagc aatataagtc tgttgatata tacaaaatat aatgactgct
     4021 ataatgagta atcaatagac acaaagagga gattatgtga tgaataataa agtattagca
     4081 accggtggta cagggtttgt tggcatgcga attatttcac gattattaga acaaggttat
     4141 gacgtacaaa cgacgatacg tgatttaagt aaagctgata aagtaattaa aacaatgcaa
     4201 gacaatggca tttccacaga gcgattaatg tttgtcgaag cggatttatc acaagatgaa
     4261 cattgggatg aagcaatgaa agattgcaag tatgtcttga gtgtagcatc tccggtgttt
     4321 ttcggtaaaa cagacgatgc agaagtgatg gcgaagcctg caattgaagg tatacaacgt
     4381 attttaagag ctgcagaaca tgcgggtgtt aaacgtgtgg taatgactgc aaactttggt
     4441 gcagttggtt ttagtaataa agataaaaat tcaatcacaa atgaaagtca ttggacaaat
     4501 gaagatgaac caggcttatc agtatatgaa aaatcaaaat tgttagctga aaaggcagcg
     4561 tgggattttg ttgagaatga aaatacaaca gtagaatttg ccacaatcaa tccagttgca
     4621 atttttgggc catcattaga tgcacacgtt tcaggaagct ttcatttatt agaaaattta
     4681 ttgaatggtt caatgaaacg tgtaccgcaa attccgttaa atgttgttga tgtgagagac
     4741 gtagctgaac tgcacatttt ggcaatgaca aatgaacaag ctaatggcaa gcgatttatt
     4801 gcgacggctg atggacaaat taatttgttg gaaattgcaa aattaattaa agaaaagaga
     4861 cctgaaatag ctcaaaaagt ttctactaaa aaattaccag actttatttt gagtctaggt
     4921 gctaaattta atcatcaagc taaagaaggt aaacttttat tagatatgaa ccgcaatgta
     4981 agtaacgaac gtgcaaaaac attacttggt tgggaaccga ttgcgacaca agaagaagca
     5041 attttagcag ctgtcgatag tatggctaag tatcatttaa tataatacaa aacaccgtcc
     5101 ataagtaagt ggctttcctc ttgataaaag gggcattcac tcaatatgtg acggtgtttt
     5161 atatttctac gttaacacta ttgctgttct ttttgacggc cttttaataa aattgttgtc
     5221 gcacctacga taataataaa tagaatcgca ccaaataatc ccatatattt tactgcgtta
     5281 ccgaacacga taccgacagc taaaaagtct gtatctgaga atgttgttgc agcaccacct
     5341 aattcgccta aaaatggcaa gaataataat ggtaaaaacg tgattaggat accatttaga
     5401 gcggcgccag caatagcacc tttaataccg cctcttgcat taccgaatac agcagccgtt
     5461 gcacctaaga agaagtgtgc aactacgcca ggtaaaatga cgacgccacc aaataagaat
     5521 aagataaaca taccgatgac acctgtaata aagctgacaa agaatccaat taatactgca
     5581 ttttgtgcat aagggaacac aatagggcag tctaatgcag gtttagaatt tggtacaagc
     5641 ttttcagaaa ttcctttaaa tgctgggacg atttcagcta agattaaacg aacgcccgtt
     5701 aaaataataa atacaccagc agcaaatgtc acaccttgaa ttaatgaaaa gacaataaag
     5761 ttttgaccat cactaataga ttcgtgtaca taactaacgc ccgcaaataa gcatgcgatg
     5821 aagtaaagta atgccatcgt aatcgagata ctaattgtac tttctcgtaa gaaacttaag
     5881 ccttttggaa atttaatctc ttccgttgat ttagacttac ctttgaataa ttgacctaca
     5941 gcacctgcgg caaagtaact gattgagcca aaatgaccta aagctacttg gtcattccct
     6001 gtaatttttc gcatcgtagg ttggagtaat gcaggtaata ctgccatgat taatcctaat
     6061 acgagtgcgc cgataacaat cgttagccag cctttaatat gactgactgt taaaatgatt
     6121 gctaaaaacg cagccatgta aaatgtatga tgacctgtta aaaagatata ttttaaatta
     6181 gtgaagcggg caattaaaat attaacaatc atgccacaga ccatgatgag tgcagctgtt
     6241 gttccaaaat cttttaaggc tagtgagacg atagcttcgt tgttaggtac gataccttgc
     6301 acaccaaatg cgtgttggaa tattttgccg aatggttcaa gagatcgaac gacgacatca
     6361 gcacctgcac ttaaaattaa gaagcctaat atcgttttaa tggttcctga agtgatcgtt
     6421 gcggcaggtt ttttctgaac gattaaacct ataaaggcaa tcagtgcaac aagaatggct
     6481 ggttgactta aaatatcgac tataaaatta aggattgctt gcataggtcg tacctccttt
     6541 aaatcatgtt aagttgttgt aatttttctg agagcttttg ttgtaattca gctttatcta
     6601 aaatattatc aagaactaag acatccccta gacgttcggc attttcagct aaatctctac
     6661 cacaaataaa caagtcagcc atctctggac ttgctgtcat aatgtcacta tgttcaactt
     6721 cgatatcaga tggtgcatta agttgcctaa gtgcttcttg tgcgttcatt tctaccataa
     6781 aactacttcc taaaccgtgg ccacatacta ctaaaatttt catattaatc atgctccttt
     6841 aaaatgtttt taatgtcttg tgcatttgtt gcagttaata gttgctggac tgtttggtta
     6901 tcgcccagta cggttgctaa attttgtaat acagataagt gtgaatgatt gtcgatggca
     6961 ctcaatacaa aaatgagaga tgcgtagtga tcttcatcac aaaatgccac atgttgattc
     7021 aactttaata gacttaaacc aacttgatgt acgtcattgt tcggtcttgc atgtgcaatt
     7081 gcaatttcag gtgcgataac gatataaggt ccaagttcat taacgctatc aatcattgct
     7141 tgaacatagc cttgttcaat aatttgttct tgtagtaatg gctgagaagc tatagttata
     7201 gcttcagtcc aatcatttac ttgttctttt acaatgatgc gtgttgttga caaaatgtct
     7261 aatgacacgt tattaagcct cctttgtcat agttaaagca atgtgttgtt taattttaaa
     7321 aatattccca tctaagaaat cttgtcgata taagtcgttg cttaagcatt cgcttaactg
     7381 tcccaatgcc tttaaatgtg cattggggtg gtccgttgct aatgtaatta caaggtgaac
     7441 gggatcgtta gctttactac caaagataat cccttcagtg aaatatgtta gtgcgaaacc
     7501 tacaccattc tgtacataat cagtaccagc gtgaataagt gcaatatgtg gactaatgac
     7561 catatatgac ccgaattgtt caaattgttt taaaattgca gctgtataat ttgaatagac
     7621 aatgccatca ttgattaaag gttgcacagc cactgcaatt gcggattcaa ttgataatgg
     7681 ttgtttattt ataatgatgc gatgttcagg caataaatct gcgagtgact tgccatcagt
     7741 tgccattttc atgactcgtt gttctcttga gtcattgata atttgattca atttttgacg
     7801 agattgttga ttgataaatg gatcgacatg aataactggt acagctgata tttcacaagg
     7861 tactgttgaa atgacataat caatgttatc ttgcaataat cgactttctt ccaattgata
     7921 aatggaatag gcatcccaaa tgtgaaactc aggatacagg tgatttagtt ttgattttaa
     7981 aagttgtgac gtgcctatac cagaaccaca tagtaagaca accttaatca ttgattgttt
     8041 atgtgttgca acacgctcta tacttgatgc gaagtgaatt gtaatgtatg ttaattcatc
     8101 ttcgttgaag cgaatagcag catcttgttc aattggacta atatgcttgc taacggcttc
     8161 aatgatttga ggatagcgac gcataacttc ttgcctcaaa ggattaggtt gtagcatatc
     8221 gtatttaata cgatgtatag ctggtttgat atgtgtgatc agactggtat gtaacttgtt
     8281 gtcttttgac atatcaatgc ctaattcttg gctaacacaa gtgatcaatt catgtatatt
     8341 ttgcgataaa tcatggtatt caaaggtaat tgaagatgct gtatgttcag tcattttaga
     8401 gcctagtaaa tgtaacgtga taaagataat ttcagactct ggaaatgtga cattacaact
     8461 gcgttctaag ttttctatca tttttgaagc aatagcatac tgattagtat gtcgccattt
     8521 atcaatttca ttgataggta tatcgaacga aaaattttca tttaaacgct gaatggcaat
     8581 gagtatatga tagattaagc catcgatagc cgactgaact aaatgataat tttcactatt
     8641 taatgtctta ataatggcac ggcgaaccaa tgcgattgat tctgaattaa agatatccgc
     8701 ctctataaaa ggtgcagctt gtttcatata ttgatgtata aagtgtgcat acgctttacg
     8761 ataatgatct tcctcaccaa taatattgaa tcctttattg tggacataat ttaactttaa
     8821 atggtattga tctagttggg cttgaatcat tttaatatca tctgcaattg tccgacgcga
     8881 aacattaaca tcttgcgcaa gttgctttgt tgaaacagga tcggttgttt cgaataactt
     8941 taaagcgata tgtgtgagtc gttcatcttt tgaaaaatga atttgatttg ttagattgtg
     9001 ctctaattca tttaatagtg tcgcgtgagc tgttgttact ttgatgcctg cagctttatt
     9061 acggctgact tggtaatgat aagtttcagc atattgctca atatatgcta tatcatattg
     9121 aatggtacga ggtgatacac caagttgatt agcaatggta ttgattggaa taaacgtttg
     9181 ctcatgaatt aaaagataca aaatttcgat ttgtctataa cttaacaacg taatatcctc
     9241 ctatttgtaa ttgtaagcga tttcttaaaa acgtagatat gcaatctctt tcatatttta
     9301 atccgaaaaa ttacatatca aaatgtttat ggcgcaagat tttataggaa cttttaaaat
     9361 aaattagata ttcatgttgt caatttaaaa atgtcgcagt atatttagtt agacatctaa
     9421 cgaaatggtg gtgcaataaa tggaattcac ttattcgtat ttatttagaa tgattagtca
     9481 tgagatgaaa caaaaggctg atcaaaagtt agagcaattt gatattacaa atgagcaagg
     9541 tcatacgtta ggttatcttt atgcacatca acaagatgga ctgacacaaa atgatattgc
     9601 taaagcatta caacgaacag gtccaactgt cagtaattta ttaaggaacc ttgaacgtaa
     9661 aaagctgatc tatcgctatg tcgatgcaca agatacgaga agaaagaata tagggctgac
     9721 tacctctggg attaaactcg tagaagcatt cacttcgata tttgatgaaa tggaacaaac
     9781 actcgtatcg cagttatctg aagaagaaaa tgaacaaatg aaagcaaact taactaaaat
     9841 gttatctagt ttacaataaa tgataagtgt gactggtaga aatcagtcac tttgtcttta
     9901 atattatagt tagatatcta attgttagta agctaattat tggaaaagac aaggagtatt
     9961 gaacaatgaa agacgaacaa ttatattatt ttgagaaatc gccagtattt aaagcgatga
    10021 tgcatttctc attgccaatg atgataggga ctttattaag cgttatttat ggcatattaa
    10081 atatttactt tataggattt ttagaagata gccacatgat ttctgctatc tctctaacac
    10141 tgccagtatt tgctatctta atggggttag gtaatttatt tggcgttggt gcaggaactt
    10201 atatttcacg tttattaggt gcgaaagact atagtaagag taaatttgta agtagtttct
    10261 ctatttatgg tggtattgca ctaggactta tcgtgatttt agttacttta ccattcagtg
    10321 atcaaatcgc agcaatttta ggggcgagag gtgaaacgtt agctttaaca agtaattatt
    10381 tgaaagtaat gtttttaagt gcaccttttg taattttgtt cttcatatta gaacaatttg
    10441 cacgtgcaat tggggcacca atggtttcta tgattggtat gttagctagt gtaggcttaa
    10501 atattatttt agatccaatt ttaatttttg gttttgattt aaacgttgtt ggtgcagctt
    10561 tgggtactgc aatcagtaat gttgctgctg ctctgttctt tatcatttat tttatgaaaa
    10621 atagtgacgt tgtgtcagtt aatattaaac ttgcgaaacc taataaagaa atgctttctg
    10681 aaatctttaa aatcggtatt cctgcatttt taatgagtat cttaatggga ttcacaggat
    10741 tagttttaaa tttattttta gcacattatg gaaacttcgc gattgcaagt tatggtatct
    10801 catttagact tgtgcaattt ccagaactta ttatcatggg attatgtgaa ggtgttgtac
    10861 cactaattgc atataacttt atggcaaata aaggccgtat gaaagacgtt atcaaagcag
    10921 ttatcatgtc tatcggcgtt atctttgttg tatgtatgag tgctgtattt acaattggac
    10981 atcatatggt cggactattt actactgatc aagccattgt tgagatggcg acatttattt
    11041 tgaaagtaac aatggcatca ttattattaa atggtatagg tttcttgttt actggtatgc
    11101 ttcaagcgac tgggcaaggt cgtggtgcta caattatggc cattttacaa ggtgcaatta
    11161 tcattccagt attatttatt atgaatgctt tgtttggact aacaggtgtc atttggtcat
    11221 tattaattgc tgagtcactt tgtgctttag cagcaatgtt aatcgtctat ttattacgtg
    11281 atcgtttgac agttgataca tctgaattaa tagaaggtta aatatttcgt ccacttctgg
    11341 ctgagtatat ttcggtcgga agtgtatttt tcgaaaaaaa taaatatatg atacgattat
    11401 gaaaaaataa agtgagattg gcatatgtgt aaatctaaaa tactgttgaa aaatattttt
    11461 agtgaagaat cagaagttaa agatttaact gaagaaaaat ataatcaaga ttacgaagca
    11521 ttaacattta gctttaaaga ggaaacatat caaagtaggt tagctaagaa aacaccgact
    11581 aaatcgggat atttcgtgac atgttggaca aaagacgaag ataattataa tcggccatac
    11641 aaaattgaag agtttgctga ttacctgatt gttgctgtta tcgatgatga attaaatggc
    11701 tactttctat ttcctaggga attattggta gaaaaaggta tcttagcttc atctaagtat
    11761 caagggaaaa tggcttttag agtttatcct aagtggtgta atcaattgaa taaaacagca
    11821 gggcaaacac aaaagtggca atgtaaatat ttttttgaat actaataaaa agtcatatgg
    11881 ttgaacttat aagagatgaa tatatcatct ttcgataaga taaccatatg actttatttt
    11941 ttatcattta atgatgaacg gtttcttgtc ctactttatt ccaagtgagg ataaagctca
    12001 acattgcaaa cacactaatt gctgttaata aaataaaacc gacatcccat ccgaatttat
    12061 caactacagc acctaagacg atgttggcca ttacagcacc aaacagataa ccaaataatc
    12121 ctgttaatcc agctgctgtg ccagctgctt ttttaggtac ataatctaat gcttgtaaac
    12181 caattaacat aactggtcca tatattaaga aaccaatggc aattaatgag acattgtcta
    12241 accaagcatt gcctggagga tttaaccaat aaattaatac aaatactgtg acacctaaca
    12301 taaagaagaa acctgcaggt ccacgacgac ctttgaataa tttatcagaa atgtaaccac
    12361 ataataatgt accaggaatt ccagcccatt cgtataagaa gtatgcccaa cctgatgctt
    12421 ttaagtcgaa atgtttttct tcacttaagt agactggcgc ccaatcaagt acaccataac
    12481 gcacgaaata aacaaatata tttgcaaagg caattgccca tacccattta ttgttcagta
    12541 catatttaaa taaaatttct tttgtagtta attctgtttc taatgttttc ttatcgcttg
    12601 tagcaaagtc atttttataa atttcgattg gaggtaaacc ttgagattga ggtgtgtctc
    12661 taatcaatac gtatgaaatt gcggcaatga taagtgctaa gagtgcaggg taaatgaata
    12721 caccttcgaa accttttaaa taaccaaagt tgataaatgc agttgttgta ataccccaag
    12781 cagcaatagg tgccataata cctccaccaa cattatgcgc aacgttccaa agggcagtct
    12841 tacttccgcg ttcacttaca ctaaaccagt gaacgagaac acggcctgaa ggtggccagc
    12901 ccataccttg aaaccatcca tttaagaata ataggacaaa cataataccg atacctgatg
    12961 tgaagaacgg tacaaatccc attaacaaat tgacgatagc agtgagtgct aatccaagaa
    13021 ctaagaatat ccgagcattg ctccgatcac ttacagtacc cataaagaac ttactaaatc
    13081 catatgcgat ggaaacagca gaaagtgcaa aacctagttc cgcttttgta aaaccttgct
    13141 cttgcaatgc cggcatcgct aacgaaaagt ttttacgtaa taaatagtac ccagcataac
    13201 cgatgaaaat accaagaaat acttggagac gtaatcgttt ataggtatca tctatctgat
    13261 tttctggcaa aggcttaata tgctttgcag gtttaagaaa attcataaaa tcctccttaa
    13321 tatgtattta tatgcatttt gtgcgataaa cttatattag acatgtatga aagcgttgtc
    13381 aatatgcttt tgtgaaatct tgcatattta gttattgaag tattttataa acatttagaa
    13441 aaaataaatt gctacacata aatttataat tatgcaatta ttatgtcgtt atacatacta
    13501 gctgtgtatc tacgaatgag tacaaacata tttttatttg caaggggtaa atggcatata
    13561 actatctttt ttatgtaagc tggtataaaa tttatactaa taggagggat agtatgaata
    13621 tagtagggca tcatcacata tccatgtata caaaagatgc aaaacgtaat aaggattttt
    13681 acacaaatgt ccttggatta cgattagttg aaaagtcggt taatcaagac aatccttcaa
    13741 tgtatcattt gttttatggg gacgaagtag gtacagccgg aacaatttta agcttttttg
    13801 aaattcccaa tgcgggtcat aagcagccag gtactgaaac gatttatcga ttttcattat
    13861 tagtaccaaa tcaagcggca cttcattatt ttgaaaaacg tcttgagaat aatggtatta
    13921 agtctgaacg tttgtactat cttggacaag aaggtgttgt ctttaaagat gaagacgact
    13981 tagaaatcat attgcttgtt aatgatagtt ttgaagtacc acatcaatgg caacataacg
    14041 cttatagtga aatacctcaa gcatatcaaa ttttaggaat agggccagtc gaattaagag
    14101 ttagaaatgc agcgcgtacg gtagaatttt tggaaaatgt cttaggttat cgcaaaagag
    14161 ataataaatc attcgatgtg ctgacattag caccacaagg tttatattcg gattttgtag
    14221 ttattgagca acagggacaa cgtgaaagac ctggacgagg ttatatccat catattgcag
    14281 ttaatacacc acaaatgagt gacttagatg caatttacaa gaaattacaa caacaaccac
    14341 aaagtaattc aggtataatt gatcgctatt tctttaaatc attatactat cgccataatt
    14401 caattatgta tgaatttgcg actgaagcgc ctggatttac tattgataca cctgttgaac
    14461 aattaggaag tcaattgaac ttgcctgact ttttagaagc agaacgtgaa caaattgaaa
    14521 gtaagttaca cgaaatataa aggagaatgt ttaatggcca aattagaaat gaataaaaat
    14581 acgcctcttg agtttggttt gtattcctta ggtgatcatt tattgaatcc attgaaaggt
    14641 gaaaaagtta gttatgagca acgtattaat gaaattattg aagcaagtaa attagcagat
    14701 gaagcaggta ttgatgtttt tgcagttggt gaaagtcatc aggagcattt tacaacacag
    14761 gcacatacgg ttgtgttagg tgcaattgcc caagcgacaa agcatattaa agtttcaagt
    14821 tcttcaacga ttattagtgc aacagatcct gtaagagtat ttgaagactt cgcgacatta
    14881 gatttgattt ctcatggtag agccgaaatt gtagctggca gagcatcaag aacaggtatt
    14941 tttgacttgt ttggctatga tttaaaagac tatgatgaat tgtttgaaga aaaattaggt
    15001 ttacttttag agttaaataa aactgagcgt attacttggt ctggaaaata tcgtccagaa
    15061 cttagaaata tgaaaatatt cccaagacca atcgataata tattgccaat atggcgtgct
    15121 gttggtggtc cacctgcaag tgctattaaa gcgggaaaac aaggtgtgcc aatgatgatt
    15181 acaacccttg gtggcccagc aatgaacttt aaaggttcta tagatgctta tcgtcaagcg
    15241 gcaactgaag caggtttcga tgcttcgcct aagtctttac cagtaagtac agcgagtctg
    15301 ttttatacag ctgaaacaac tcaggatgct atgagagaat tttatccaca tttgaataca
    15361 gggatgtcat ttattcgtgg tgttggttat ccgaaacagc aatttgctaa ttcgtcagat
    15421 tatcgagaag cgctaatggt tggaagcccg caacaaatta ttgaaaagat attgtatcaa
    15481 cacgagttgt atggtcatca acgttttatg gcacagcttg attttggcgg tgtgccattt
    15541 gaaaatgtta tgaagaatat tgagttaatt ggcaacgaca ttataccggc gattaaaaag
    15601 catttatcaa aataggaggg gcgtcatcat gaatattgta ttattgtcag gttccacagt
    15661 aggttctaaa acgagaattg ctatggatga tttaaaaaat gaactagaag tcatcaatga
    15721 gggacatcaa atagagttga tggatttacg agaacttgaa ttagaattta gcgttggaaa
    15781 gaattatcta gatactacag gagatgtata taaattaacg acgtcgttaa tgcaggctga
    15841 tgtgattttt attggttttc caatttttca agcttccatc cctggtgctt tgaaaaatgt
    15901 gtttgatcta cttccagtca atgcgtttcg tgacaaggta ataggacttg tagcgacagc
    15961 aggttctagt aaacattatt taattcctga aatgcattta aaaccaatat tgagttacat
    16021 gaaagcacat acgatgcaaa cgtatgtatt tattgaagag aaagattttt caaatcaaca
    16081 aattgtcaat gatgatgttg tatttcggtt aaaagcgttg gcacaatcca caatgcgaac
    16141 tgccaaagta caacaacaag tgtttgaaga agaaaacaac caatacgact tttaaagtat
    16201 aaaaataaga cgctcggcac actaaatttg taagtgtttg agcgtctttt catattaact
    16261 atatagccaa tgaacgacga taaaggcaag tgatgacaag catattgagg taataatgat
    16321 tgtcataagc ggtttaagtg cgcgattttt aagatcttta aatgcaacat ttaaccctaa
    16381 agcaaccatg gccattaata agcaaattgt tgatacagta tttaaaatat ttagcaatgc
    16441 tgacggaata gttacatatg tattcactaa ggccataatg acaaatccaa ttaaaaagta
    16501 tggaatgctt attcgaccct tgctagatga ttctgatgaa cggaaacgca taattaaaat
    16561 aagtacgatg gttaatggaa tcagtaagaa tactctacca agtttaccaa gaagtgcaat
    16621 tttaagtgca tcactaccac caaagccacc agctaagaca acgtgtgcaa tttcatgaag
    16681 actaacacca gaccaagcgc cataaacatt tgtcgtcatt gaaaagatag cgtagatagc
    16741 tgtatatata agtgaaaata tcgtaccaat caatgcgatg ataccgatac taatagctgt
    16801 atccttttca cgtgatttga atattggagc gactgcggca atagcagcag caccacaaac
    16861 gcctgtgccg acacctagta ataatgcgat gtttttgtca ccatgcaaca gtttgttgac
    16921 aaagagcatc attacaatac tgaaaataac gacacctaca tcgatggcta atagtttact
    16981 accttgaccg ataatatcga atatattgag tttaagtcca tataggatga ttgcaaatct
    17041 taataaatat ttagatgaaa acgtaatacc tgagctatat tgttcaggat atcctctaaa
    17101 gtgacgatat agaatagcga ttaatatcgc gatagttaat gcgccaacct tatctaggat
    17161 tggcaattta gctgctaaaa agctaaataa tgcgactata aatgttagtg atagcccaat
    17221 cataaaatgc ttatttttca atgatgccat gagcagtgcc tcctttaata gcattttagc
    17281 actgttttgt cgtattttta aatataaatt tggaatgaat aataaagtag tgattaaatt
    17341 aagttgtgtg ataggaaact tggacatcaa tcaaagtaat aggcactaca acgcttattg
    17401 gc
//
LOCUS       DANRDI010000044        16295 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.044, whole genome shotgun sequence.
ACCESSION   DANRDI010000044 DANRDI010000000
VERSION     DANRDI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.044"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..66)
                     /locus_tag="PZ377_001150"
     CDS             complement(<1..66)
                     /locus_tag="PZ377_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose pyrophosphatase"
                     /protein_id="HDM6358031.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVE"
     gene            148..1056
                     /locus_tag="PZ377_001151"
     CDS             148..1056
                     /locus_tag="PZ377_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDM6358032.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(1272..1520)
                     /locus_tag="PZ377_001152"
     CDS             complement(1272..1520)
                     /locus_tag="PZ377_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358033.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(1536..2291)
                     /locus_tag="PZ377_001153"
     CDS             complement(1536..2291)
                     /locus_tag="PZ377_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDM6358034.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            2433..3248
                     /gene="proC"
                     /locus_tag="PZ377_001154"
     CDS             2433..3248
                     /gene="proC"
                     /locus_tag="PZ377_001154"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDM6358035.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(3353..4273)
                     /gene="rnz"
                     /locus_tag="PZ377_001155"
     CDS             complement(3353..4273)
                     /gene="rnz"
                     /locus_tag="PZ377_001155"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDM6358036.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            4586..6070
                     /locus_tag="PZ377_001156"
     CDS             4586..6070
                     /locus_tag="PZ377_001156"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDM6358037.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            6300..7166
                     /locus_tag="PZ377_001157"
     CDS             6300..7166
                     /locus_tag="PZ377_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDM6358038.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(7249..8898)
                     /locus_tag="PZ377_001158"
     CDS             complement(7249..8898)
                     /locus_tag="PZ377_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDM6358039.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(8914..9933)
                     /locus_tag="PZ377_001159"
     CDS             complement(8914..9933)
                     /locus_tag="PZ377_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDM6358040.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            complement(9934..10188)
                     /locus_tag="PZ377_001160"
     CDS             complement(9934..10188)
                     /locus_tag="PZ377_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358041.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            complement(10127..10498)
                     /locus_tag="PZ377_001161"
     CDS             complement(10127..10498)
                     /locus_tag="PZ377_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6358042.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            complement(10717..12123)
                     /gene="gndA"
                     /locus_tag="PZ377_001162"
     CDS             complement(10717..12123)
                     /gene="gndA"
                     /locus_tag="PZ377_001162"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDM6358043.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            complement(12191..13324)
                     /locus_tag="PZ377_001163"
     CDS             complement(12191..13324)
                     /locus_tag="PZ377_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDM6358044.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            13587..13766
                     /gene="prli42"
                     /locus_tag="PZ377_001164"
     CDS             13587..13766
                     /gene="prli42"
                     /locus_tag="PZ377_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HDM6358045.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            complement(13893..14873)
                     /locus_tag="PZ377_001165"
     CDS             complement(13893..14873)
                     /locus_tag="PZ377_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDM6358046.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(14887..15324)
                     /locus_tag="PZ377_001166"
     CDS             complement(14887..15324)
                     /locus_tag="PZ377_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDM6358047.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            complement(15675..>16295)
                     /locus_tag="PZ377_001167"
     CDS             complement(15675..>16295)
                     /locus_tag="PZ377_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDM6358048.1"
                     /translation="HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVAD
                     ALKAYPLLNSSWQGNEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTL
                     ATKARNKQLTAEDMQGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVIND
                     MIAIRNMVNLCISIDHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
BASE COUNT         5332 a         2922 c         2352 g         5689 t
ORIGIN      
        1 ttctacatct acaattttgc cattataaat aactgttcga tcaattgttt tttcatttaa
       61 atccattata atcacgttcc tttaaattca tattatatat tgatacacta tgcttgttag
      121 ctaaagtgta tcgaaaggag aacagacatg caaaaaaata tattaaaaag tggtatttca
      181 ttatctgaat taggtctagg ttgcatgagt ttaggtacag atttaaaaaa agccgaacaa
      241 attatagatt gtgctgttga aaatggtatc acttattttg atacagcaga tatgtacgat
      301 aaaggtataa atgaatcagt tgttggtaag gcacttctta aatatcaaca acgcgatgat
      361 atttttattg gtacaaaagt aggcaatcgt ttaacaaaag atggcagtac aacatgggat
      421 ccgagtaaat cctatattaa agaggcagtt aaaggttcac taaagcgttt aggtatcgat
      481 catatcgatt tatatcaact tcatggcgga accattgatg acccattaga cgaaacaata
      541 agcgcatttg atgaattgaa acaagaagga attatacgtg cttacggtat ttcttctatt
      601 cgcccaaatg taattgatta ttatttaaaa catagtcaaa tcgaaacgat aatgtctcaa
      661 ttcaatttga ttgataatcg tccagaatca ttattagatg caattcacaa caatgatgtt
      721 aaagtattgg caagaggacc tgtgtctaaa ggattattaa cttcaaacag tgttaatgtg
      781 ctcgacaata aatttaaaga tggtattttt gattattctc atgatgaatt gggtgaaaca
      841 atagcctcta ttaaagaaat tgaaagtaat ttatctgcat tgacatttag ttatttaaca
      901 tcacatgacg tgcttggttc catcattgta ggtgcaagta gcgtcgacca attaaaagaa
      961 aatattgaaa actatcatac taaagttagt ttagatcaga ttaaaacagc aagagctcgt
     1021 gtaaaggatt tggaatatac caatcattta gtgtagaagt cattttcagt aataaaaaca
     1081 gcagcatgag gcgtttcatt ataaaaatgc cttactgctg ttgtttatgt acaattcgct
     1141 ataatttatg attatgatta ctcacttatg atagaaatta aagcgttgtc ctcacgcatc
     1201 agtatttagt aatttcgcct tgcggcattg ccttaagcaa acttctgcca cttcatctct
     1261 taataatttt attaaaacat ctttctatat ttcacttcgc atgttgattc atcattatta
     1321 gttattattt gtacacccag cacatttcct tgcaacacaa gtagtttgaa tttttcacaa
     1381 gtataatata atgtaccgtc tgaaatttgg tctacagaaa tatcgcctaa aatatccagc
     1441 actgtaaatt cttcaaatac tgatagttgt tccgcatatc gtacacaaag tcttaccaca
     1501 ctctccgatt gacagttcat tgccatccca cctatttatg ctttattttt aaataattta
     1561 gggaaacatc gttcaaaaaa tctaggcgca atttgataca ttttcaacgc atgatgcatc
     1621 catttaggcc gattaatttc caattgtttt gttttaatgc cataaatgat atcttctgca
     1681 agctgattag catcaagcat aatttccccc atctttttag catacttcat tgatgggtcg
     1741 gctttttgat gaaaaggtgt atcaatcggg ccaacattaa ctgtcatgat atgtaagttt
     1801 ggtgactcta gtcttaaagc attcattaat gcataaaacc ctgctttcga tgccccataa
     1861 tgtgcagcat ttgcttgtgt ggaaaatgca gcttgacttg aaatacctac aatatgtgcg
     1921 ttagatgtta aatatggtct caacacagta tataaaacat taaaactaat taaattaagc
     1981 tgatacgttt caatcatttc tgaaaaacta tggtctgaaa tagatttgaa ataacctaaa
     2041 cctgcactat aaatgaatcc atcgaatgat gtattgtctt caaattgcag tgcctgtatc
     2101 gacttcaaat catttaagtc acaaggaata acatttatag ttttccccaa ttcctgttca
     2161 aagattctag ttgctttatc aacatcacgc accaacaacg ttacatgcac tttattttct
     2221 agtaactttc ggacaatcga taaacctaaa ccactcgtac caccagtcac tataaaatgt
     2281 tgtcctttca tcaattaacc ttccttttca attatataga atgcaattta tcaactttac
     2341 ataattgaga caagttgatt atctttccta atatatatac aataataaga aaatataaca
     2401 tacaaatcaa aaactaaagg gatgtgacgt taatgaaact cgtattttat ggagctggta
     2461 atatggcaca agctatattt acaggaatta ttaactcaag caacttagat gccaatgata
     2521 tatatttaac aaataaatct aatgaacaag ctttaaaagc attcgctgaa aaactaggtg
     2581 ttaactatag ttatgatgat gcgacattat taaaagatgc agattatgta tttttaggta
     2641 ccaaaccaca tgattttgat gctctagcaa cacgcatcaa accacatatt acaaaagaca
     2701 attgcttcat ttcaattatg gcaggtattc cgattgatta tattaaacaa caattagaat
     2761 gccaaaatcc agttgctaga attatgccaa acacaaatgc gcaagttgga cactctgtta
     2821 ctggcattag tttttcaaac aactttgacc ctaaatctaa agatgaaatt aacgatttag
     2881 ttaaagcatt tggttctgta attgaagtat cagaagatca tttacatcaa gtaacagcta
     2941 tcaccggaag cggcccagca tttttatatc atgtattcga gcaatatgtt aaagctggta
     3001 cgaaacttgg tctagaaaaa gaacaagttg aagaatctat acgcaacctt attataggta
     3061 caagtaagat gattgaacgt tcagatttga gcatggctca attaagaaaa aatattacct
     3121 ctaaaggtgg tacgacacaa gctggccttg atacattgtc acaatatgat ttagtatcta
     3181 ttttcgaaga ttgtctaaac gctgccgtcg accgtagtat tgaactttct aatatagaag
     3241 accaataaaa acaaacccgc caacacatgt atgcatcatc gcaagcactg tgtttgacgg
     3301 gttattttta taatttattg ttatttggca agcattgttt attactttgt cattagattt
     3361 taaaactatc aaaatctttt acaaaattaa aattaggtgt atcttcattt tgtatcaatg
     3421 tttgataaat ttcatttata tcttctgtat tatagcgatt gctcaaatgt gtaatcaacg
     3481 tacgtttaac attggcttct tttatcaatg caaatacgtc ttcaatatgg ctatgatgat
     3541 aattgttggc taaatgcttt tcaccatcta tataggtcgc ttcatgtacc atcacatcag
     3601 catctctaga aatcacacgt tcattagaac atggttttgt atcaccaaaa attgctacaa
     3661 ctggaccctg tttggactca cctctaaaat cttttgattg ataaacttga ccattatgtt
     3721 caaatgtatc atgagatttt acttcttgat atttaggacc tggttcaaga ccaatgtttt
     3781 ttaacgcttc aacattgatt gtacctgtag tttcaggtgc cattactcta tatccatatg
     3841 atggaatacc atgattaagt aaatgcgcct ctacagtaaa accatcatga tgatatgtca
     3901 gatgatcatc gatttcaata tatgtaattg gatagtttaa atgtgactct gataaattca
     3961 tagacatttc cacatatgct ttaattcctt ttggtccaac caatgtaagc ggcttctgtt
     4021 caccgccctg aaaagaacga ctagaaagta atcctggcaa accaaaaata tgatcgccat
     4081 gcatatgagt aataaatata tgtgtcactt ttcctaattt aattgcatga tgtaaaattt
     4141 ggtgctgtgt accttcacca acgtcgaaaa gccatatgga attggaatat ggttctaaat
     4201 ttaaggcgat tgcttgtgta tttctctctt ttgtaggcaa acctgcactc gttccaaaaa
     4261 atgtaacttc catatatgcc cctccttttc ttcaattcat tttatcataa aatttgtatc
     4321 aatttagttt taatttatac ttgaatcaaa atctgttaaa aattcgtctt tgctagttaa
     4381 tttttctaac tttaacgtag acataactat ataaattttg ataattacgt tatacttatc
     4441 attaataagt atcacattaa acatgataca tgaatcgata tttcatttaa gacactgcat
     4501 acagtcgagc atattgtatg acctactgaa tggattatct tataataata aatcatatat
     4561 ctaattaaga attgaggttt taatcttgag tactaaaaac aaacacatcc catgtttaat
     4621 cacaatcttt ggtgcaactg gtgacttaag ccatcgtaag ttgtttccat caatattcca
     4681 tctctaccaa caagacaatt tagatgaaca tattgccatc atcggtattg gacgtcgtga
     4741 cattactaat gatgatttcc gtaatcaagt aaaatcatca attcaaaagc acgtaaaaga
     4801 tacaaacaaa attgacgcgt ttatggaaca tgtcttctat catagacatg atgttagtaa
     4861 tgaagaaagc tatcaagaat tactagattt tagtaatgaa ttagatagcc aatttgaatt
     4921 aaaaggtaat cgactattct atttagcaat ggcaccacaa ttctttggcg ttatttctga
     4981 ttatctaaaa tcttctggtc ttactgatac aaaaggattt aaacgccttg ttatcgaaaa
     5041 accattcggt agtgatttaa aatcagccga agcattaaac aatcaaattc gtaaatcatt
     5101 taaagaagaa gaaatttatc gtattgacca ctatttagga aaagacatgg ttcaaaatat
     5161 cgaggtatta cgttttgcga atgcgatgtt tgaaccatta tggaataaca aatatatttc
     5221 aaacatccaa gttacatctt ctgaaatact aggtgttgaa gatcgtggtg gttattatga
     5281 atcaagtggc gcgctaaaag atatggtgca aaaccacatg ttacaaatgg ttgcattatt
     5341 agctatggaa gcacctatta gtttaaatag tgaagatatc cgtgctgaga aagtaaaagt
     5401 acttaaatca ctgcgtcatt tccaatctga agatgttaaa aagaactttg ttcgtggtca
     5461 atatggcgaa ggctatatcg atggtaaaca agttaaagca taccgtgatg aagatcgcgt
     5521 tgcagatgac tctaacacac ctacctttgt ttcaggtaaa ttaacaattg ataactttag
     5581 atgggctggt gtaccattct atattcgtac tggtaaacgt atgaaatcta aaacaattca
     5641 agttgtcgtt gaatttaaag aagtaccaat gaacttatac tatgaaactg ataaactgtt
     5701 agattcaaac ctattagtaa tcaatatcca acctaatgaa ggtgtatctt tacatctaaa
     5761 tgctaagaaa aatacacaag gtatcgaaac agaacctgtc caattgtctt actcaatgag
     5821 cgctcaagat aaaatgaata ctgtagatgc atatgaaaat ctattatttg attgtcttaa
     5881 aggtgatgcc actaacttca cgcactggga agaattaaaa tcaacatgga aatttgttga
     5941 tgcaattcaa gatgaatgga atatggttga tccagaattc cctaactatg aatcaggtac
     6001 taatggtcca ttagaaagtg atttactact tgctcgtgat ggtaaccatt ggtgggacga
     6061 tattcaataa ttgaattaaa acgcacatgt taaacaaaaa taaatgagcg aatgacaagg
     6121 attgattacg aatcaagtaa atcaattgtt ctatcattcg ctttttttct agactatatt
     6181 atgaaattat attttacaat gcccaaaact attttaataa tcattgaaca aatgggtgta
     6241 taatttatag aaataatgta gaataaaaat aaatgattga attaattgga gtgaaagttt
     6301 tggacgttat caagcaaata caacaggcaa ttgtttatat tgaagatcgt ttattagagc
     6361 ctttcaattt gcaagaatta agtgattacg ttggtctttc gccataccat cttgatcaat
     6421 catttaaaat gattgtcggc ttatctccag aagcttatgc acgcgcgcgt aaaatgacac
     6481 tcgctgcaaa tgatgtgatt aatggtgcta cacgacttgt agatatcgct aaaaaatatc
     6541 actatgcaaa ttcaaatgat tttgcaaatg attttagtga ttttcacggc gtatcaccta
     6601 ttcaagcctc tactaaaaaa gatgaattac aaattcaaga gcgattatat atcaaattat
     6661 caactactga gagagcacct tatccataca gattagaaga gacagatgat atttcattgg
     6721 ttggatatgc acgatttata gacactaagt atttgtcaca tccttttaat gttccggatt
     6781 ttttagaaga cttgctcatt gatggtaaaa ttaaagagtt acgacgatat aatgacgtta
     6841 gtccatttga actatttgtt attagttgtc ctcttgaaaa tggtttagaa atatttgtag
     6901 gtgtaccaag tgaacgttat cctgcacact tagaaagtcg atttttacct ggcaaacatt
     6961 gtgcgaaatt caatttacaa ggtgaaattg attatgcaac taatgaagct tggtactata
     7021 ttgaatcaag tttgcagtta acattgccat atgaacgaaa tgatttatat gttgaagtgt
     7081 accctctcga tatttcattt aatgacccat tcactaaaat tcagctttgg attcctgtta
     7141 aacagagtcc ttatgacgaa gattaaataa taaaaaacaa agaagccccc taatatatct
     7201 ataggtctac aaatggcctt agattctatt agggggcata ttaatatgtt aatttagttc
     7261 gataacacat gcttcatatg gacgtaactg ttttaaatta actttggcat cataattaaa
     7321 tagctttact tctccatggc ttaaatcaaa tggtacagtt aattctgctt cgtggttagt
     7381 aagattacct acaataagaa cttgcttttc atttaatgtt ctcgtgtacg caaaaacttg
     7441 tgaattttca gcatctacta aatcaaattg accatatacg tatacatcat tagactttct
     7501 taattgaatt aaatctttat aaaattgtaa tactgaatgc tcatcttcta attgttgtgc
     7561 aacattgata gttttataat tcggattcac tgggaaccac ggttcaccat ttgtaaatcc
     7621 tccatttaac gtatcatccc attgcattgg tgtgcgagaa ttatctcggt tctcatcttt
     7681 atatttcgca agtaaagcgt ctacatctcc accttgagct ttcactattt gatagtcatt
     7741 tttaacagca acatcgttaa acgtttcaat actttcaaat ggataattcg tcataccaat
     7801 ttcttgacct tgataaatga atggcgtacc ttgttgcaag aaataaacag ctgcatgact
     7861 tgttgctgat tcataccaat acttgtcatc gtcaccccac gtcgatacac gtcgtggttg
     7921 gtcgtgattt tcaataaaca acgcattcca acctttattt tcaagttgtt tttgccatct
     7981 atttaataca gatttatacg aatttacatc aaagtgagaa tcaccactat tccacagtcc
     8041 caaatgttca aattggaata tcatattaaa tttaccattt tcttccccga cccagtcatc
     8101 agcatcatca gggcttacac cattcgcttc accaacagtc ataatgtcat acttacttaa
     8161 tgagcgatct ttcatctctt gtaaccaagt ttgtatacct ggctgattca tatctacatc
     8221 aaatgctggg gcatatgttt taccctcagg tacaggtaag tcacccgctt caaacgtctt
     8281 cttaatatgc gtaattgcat ctactctaaa tccatcaatg cctttatcaa accaccagtt
     8341 catcatttca aatacagcat ctctaacttc cggattaccc caattcaaat caggttgttt
     8401 tttactgaat aaatggaaat aatattgctc agtattagca tcatattccc atgtagatcc
     8461 attaaatata ctttcccagt tgttaggttc agagccatct ggctttggat cttgccaaat
     8521 gtaccaatca cgtttgggat tgtctttact agatttggat tctataaacc aaggatgttc
     8581 atcagatgta tgatttacaa ctaaatctaa aataagcttc atgcctctat catgaacacc
     8641 ttttaataaa cgatcaaagt cttccatcgt tccaaattca tccataatct cttggtagtc
     8701 actaatatca taaccattgt catcattagg tgatttaaac attggactga gccaaatgac
     8761 atcgataccg aaatctttta agtagtccaa tttatcaatc attccaggta aatccccaat
     8821 accatcgtga ttactatcat taaaacttct tggatatact tgatatgcta ctgcttcttt
     8881 ccaccattgc ttattcattt taaaactcct ttgctatcgc tgtgttgatt ttcttatttt
     8941 taattctgta tctataatga cgagttcaat aacatcctgt gctttgtttt tcaatatatt
     9001 taaaattgct gcaccagcct gttgacctaa cattcgaggc ttgatgtcaa tacaggtttg
     9061 tggtggtgac gcaatttcgg ttaaataaga atcattgaac gttgctgtca ttacatcttt
     9121 cggaatttca atattaagtt catataggac acttaaaatc gctaaatgta acatagcatc
     9181 taacgaaatg attgcctgtt taatatttgg gtccttcaaa cgcgtatgta gattttgcat
     9241 gtaattaaaa ataacttctc tttcattact agtctcaata atttgataat taattttatt
     9301 ttgagaagct atcgtttcaa atccttgaat tctatctttt gaaacttcaa aatttccttt
     9361 ttctgtaata aatattaatt catctacacc ttgttcaata acatgtcgtg tcaaattttc
     9421 agaagctaat atattatcat tatctatatg tgtaaattga tgatctatat ccgatgtagg
     9481 cttaccaatc acaataaatg gcatgctttc atcaattaac atttgtttaa tcggatcatt
     9541 ttcttttgaa tagagcagta taaacgcatc aaccattcgt tgtttaatca ttttataaac
     9601 ttcatccatt aaatcattca tattatttga gactgtcgtt tgtgtaccat agccatgctg
     9661 gttacacgtt tcagaaattc ctagcaatac attgatgtag aatggattca gtcgaatagg
     9721 ctcctcagac ccttttaaca ctaaaccaat tttatatgtt tggtttgtaa ttaaagttct
     9781 agcagcggta ttaggaaaat aattcaattc ttccataact ttcttcactt ttgaaattgt
     9841 cgcttcgcta atacgttgat ttccttttat aactcttgaa actgtcgaag gagaaacacc
     9901 ggcttttagt gcaacatctt taatcgtaac catttaatca cctcctgtta atttctgcat
     9961 tggaaaacgc ttccaaccac tgtataatac cagtttagtc acactttcta aaaaagtcaa
    10021 aagatttgtg caaacgattg cataaaacga taaaaataaa accttcatac tgaaattcaa
    10081 tccgaaaatc aatataaagg tttgtataaa tattaaaatc gattgtttag tcactaactg
    10141 caaaatagtt accttggcca tcttgaaaat taaatacacg ttgaccattc atttctacta
    10201 tatcatgccc agttaaacct aaatcattta attttgagta taatgcatca aagtttttct
    10261 ctttaaacat taaagatggt gttcctaggt tcacttccgg gctatgcttt tcaataaatt
    10321 cttttgccat aatcgtcaat gacgtttcag catctttggt aggtgatact tcaactgcaa
    10381 catagtcctc agctaacggt gtttcactta caacaacaaa ttctaaagtt tctgtccaaa
    10441 atgctttcgc tttttcgaca tcatcaacat ataacataac ttgatttaac ttttccataa
    10501 aatagtacct ctatttctct atagtacatg ctatcataac acagtaaata ttttattact
    10561 tcacaaaatg cttaaaaata tggcgggatg cttttaaggt caaggataat acttgtgtaa
    10621 ttttttatag gttgtagcta ctctatcaca ctctctttta tatttatcaa aagatataaa
    10681 aaaggatagt atctttcaac tatcctttaa tcaatattat tcttcaatcc attgtgtatg
    10741 gaatacgcct tctttatctt ttctttcgta cgtatgagca ccgaagtagt cacgttgtgc
    10801 ttgaattaag tttgcaggta aatcagcagc acggtaacta tcatagtaat taatacttga
    10861 tgagaaacca ggtgttggta caccattttg aacaccagtt gcgacaacat cacgtaacgc
    10921 atcttgatat tcagtaacga tgtttttaaa gtaaggatct agcaataagt tttgtaatcc
    10981 tggattatta tcgtaagcat ctttgatctt ttgtaagaat tgtgcacgga taatgcaacc
    11041 ttctctccaa atcatagcta aatcaccaag ttttaaattc cattcattat cttcacttgc
    11101 tttacgcatt tgcgcgaaac cttgtgcata agaacaaatt ttactcatat ataatgcttt
    11161 acgaattttt tctaaaaagt ctttcttgtc accatcaaat gatgcttttg gaccatttaa
    11221 ttctttagaa gcatttacgc gctcttcttt gattgaagag ataaaacgtg caaatacaga
    11281 ttcagtaatg attgttaatg gaatacctaa ttctaatgcg ttaattgaag tccattttcc
    11341 tgtacctttt tgacctgcag tatcaagaat tttttcaact aatgcttctt tattttcatc
    11401 taatttcatg aaaatatcac cagtgatttc aattaaataa ctttctaatt caccagcatt
    11461 ccagtctttg aacgtttgag caatgtcttc atgagacatg cctaataatt ctttcatcat
    11521 agcataactt tctgcaatta attgcatgtc agcatattcg ataccattgt gtaccatttt
    11581 cacatagtgt ccagcaccat taggtccaat ataagtaaca catgaagcac cgtcttttgc
    11641 ctttgcagca attgcatcaa gaatatctgc aactttgtta taagcttctt cttgtccacc
    11701 cggcattaat gacggaccag ttaacgctcc aatttcacca ccagaaacgc ccataccaat
    11761 aaagttgatt gcactttgtg ctaatgcttt attacgtctg atagtatctt gatagtttgt
    11821 attaccacca tcaattaaaa tatctccatc atctaataaa ggtaacaaac tatcaatcgt
    11881 tgcgtccgta gctttacctg cttgaaccat taataaaatt ttacgtggtt tttctaaaga
    11941 attaacaaat tcttccaatg aatacgttgg atgaatattt ttcccttttg attcttcaac
    12001 cattaaatca gttttttcac ttgagcggtt aaatacagat acactatatc cgcgtgattc
    12061 aatattccaa gctaggtttt tacccataac ggctaaacca ataactccaa tttgttgtgt
    12121 catattactt acctcacttg ttgatttttc attagtattg tatcacaaaa tagacataca
    12181 ctacactaaa tcatttcgaa tgtcgcgcaa ctattttgat tatttctaac acttgacttg
    12241 caagcaagtt caatgattta atcggcattc tctcatttgt tgtatggatt ttttcataac
    12301 ccactcctaa aatgactgaa ggaataccaa atgtattaat aatactgccg tctgaaccgc
    12361 caccagaaat aattgtattt gcagataatc ctaaattacg agcactttct tgtgcaattt
    12421 taacaaccgc ttcattatca ttaattttaa atcctggata actttgctcc actgtaacta
    12481 ctgctttccc acctaattct gatgcagtag tttcaaacac atcagtcata tgtttgactt
    12541 gtgtttttat tctttctgga tcgtgagaac gtgcctctgc ttctaaaatg acttcatctg
    12601 caacaatatt cgtagctgaa ccgccatgaa acttaccaat attggcagta gttatttcat
    12661 caacttgtcc taatttcatt cgactaattg ctttcgccgc aatattaata gcactaacac
    12721 cctcttttgg cgtacttgca tgagccgttt tgccaaaaat tttagctgaa attaacattt
    12781 gcgtcggtgc acctacaacc gtagtaccga catcagcact tgcatcaata gcataaccaa
    12841 agtccgcgtc caacaactct gaatttaatt ctttagcacc aattaaacct gattcttctc
    12901 caacagtaat cacaaattga atttgtccat gtgggatttg ttgttccttt atcacttgca
    12961 aaacttcaag catcgctgct aatcctgctt tatcatctgc acctagaata gtcgtaccat
    13021 cagagtatat gtagccgtca tcttttacaa ttggctttac attaattgcg ggtacaacag
    13081 tatccatatg gctcgtcaaa tataatttag gtacttcgcc ttcttcgata gtactattca
    13141 ttgtacacac tagattattg gcacctaatt taggatgttt agccgcttca tcttctttaa
    13201 catctaaccc taatgctatg aatttttctt ttaaaatagg ttggattgtt gattcattcc
    13261 ctgtctcaga atcgatttgt acaagttcaa aaaacgtatt aagtaatctt tgctcattaa
    13321 tcataatgga acccctttta tcaaaatttt catgtacgcc aaacggtaaa aattgatgaa
    13381 ataaaatgtt acagtaattg acgttacaca gatttatcag gtttgtaaat tgtgtcatat
    13441 tattttcaat ttattatata taattattgt aactcaaact aagctttgtc aaaaatatat
    13501 tgattgattt ttcaaagata tcgtataatg aggaaaatga cataagcaaa cttactcatg
    13561 ttttttatta tattccttta tgatgattgc tagttatatc gtctcaagtt aaaagtttta
    13621 tatcttatgt cgtaattatt aatacaaagg ttattcattt ggaggcacac aaaatgcaaa
    13681 ataaagtttt aagaattatc attatcgtta tgcttgtatc agttgtatta gcattgttat
    13741 taacgagtat cattccaatt ttataaacta tatctcaact acctatacaa aatcatacaa
    13801 ttaaaaatcc atccattata aacgcatgta ttaataagtt atcgtattgc aacgattact
    13861 ttcaaacatg gctcatacgg atggattatt ttttaagcta cttcactatg cattttcaat
    13921 gaaccaaatt gcgatttgat ttgtaaatat tcttctaatt catttaatat ttgaataata
    13981 cttgctctcg agttaagcgc tttgtgtgtt gttggcaatg gcagttcatc caatttcaaa
    14041 cgcgtctcat acaaattgtg taaacgcatt gctgtatagt cattactatt cacatttaga
    14101 ccaatttctt tcagcagtga cgcaacatca tttaaaagcg gatctttatg acagatactt
    14161 tcgatgagcg gtttcattct cattaacaat tccacttgct cttctcgcat atcaaaataa
    14221 tgatagtatg aattttcgtt tctaacaaaa tgatttttaa catctcggaa cgcgatagac
    14281 ttcgcctttt taatatttaa aagtaacact tcaaattcaa tcgcaatggt atcttcatat
    14341 ttttcacaaa tataactata tttactaaaa atatcagcaa tttgttgctc aattttacat
    14401 ttgtattcgt ctagttgttt gtctaaactt ggcatcatta aattcattgt aaatgcaatg
    14461 cttagtccaa ttaacagtaa taatgtttca ttaacaatta aatgtgcatc aattgatttt
    14521 gcattaaaaa catgaagtaa tataacgcaa ctcgtaatga caccttcttg tacttttaat
    14581 acgacagtta atggtataaa taacaatacg ataataccga gtacaattgg actctgacct
    14641 aataaactaa atattgctga acctaaaaac aatactaaaa aacatgatac taatcttgaa
    14701 ataatcgctt gtagcgaatg tacttttgta tgtttaatac ataatacgac taatatggcg
    14761 cttgaagcat aattatctaa acctaacagc ttactaataa ttacacctaa agtcataccc
    14821 actgctgttt ttattgttct aaatccaatc ttgtaaggat ttaactttaa catgggttag
    14881 cgcctcttat ctttcttcac aatatttatt gaataatgtt tgtaattgat taattacgtt
    14941 catcacatca tgaccttcga tttgatgtct ttcaatcatt tctgtaatct ttccatcttt
    15001 tactaatgca aatgacggac ttgaaggcgc ataaccttcg aagtattcac gcgctctttg
    15061 tgtcgcttct ttatcttgtc cagcaaatac tgtcactaga cgatcaggta atacgtcata
    15121 atgtaaagca tgtgatgctg ctggtcttgc gataccacct gcacaaccac atacagaatt
    15181 gatcataact agtgttgtac catcttgttt aagaactttg tcaacatctt ctgcagtagt
    15241 taattgctca tatcccgcag attcaatttc attccttgct tgttctacaa caccgttcat
    15301 gtataaatcg aaattcatgt ccataagttc aatcacctat ccctttatat ttaaactatc
    15361 ctcattctac taattaataa catattgttc aataaactaa tctgaatcac acctatattt
    15421 agacacaatt ttaacaatat accaaacatt attgtgctta aaatcatggt aactaatttg
    15481 ttcacatgtt ttcattaata tgtttcaagt atgatgtctt attttgactt tactgcaaaa
    15541 atgcattcaa ccatgttgat tattgttctt tatctttttt gaatatattg cacatatttt
    15601 agtgccaaaa aataatacat ccatcgacaa gaacaagata aaacaagttg tcgatagatg
    15661 catctatgtt atcactaata tatatttgta ttttctaaag tatactgttc gatacgctgt
    15721 ttaatatgat tcataaattt acctgtttgt aaaccatcta aaatacgatg atcaattgaa
    15781 atacataaat taaccatgtt acgaattgca atcatatcat taattactac tggcttttta
    15841 atgattgatt ctacttgtaa aatcgctgct tgtggatgat ttataatacc cattgatgat
    15901 actgaaccaa atgtaccagt attatttacc gtaaatgtac cgccctgcat atcttcagct
    15961 gtcaattgct tattacgcgc tttcgttgct aaagtattaa tttctctagc tatacctttg
    16021 attgactttt cgtctgcatg cttaatcaca ggtacgtata atttattttc atcagcaaca
    16081 gcaattgaaa tattaatgtc tttatgtaag acaatttcat ttccttgcca gctactattt
    16141 aataaaggat atgcttttaa agcatctgct acagctttta caaagaaagc aaagaacgtt
    16201 agattatatc cttctttatt tttaaagctg tttttataat gatttctcgt attcacaaga
    16261 tttgtagcat ctacttcaat catcatccat gcatg
//
LOCUS       DANRDI010000045        16215 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.045, whole genome shotgun sequence.
ACCESSION   DANRDI010000045 DANRDI010000000
VERSION     DANRDI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.045"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(53..1207)
                     /locus_tag="PZ377_001168"
     CDS             complement(53..1207)
                     /locus_tag="PZ377_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDM6358049.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(1403..2413)
                     /locus_tag="PZ377_001169"
     CDS             complement(1403..2413)
                     /locus_tag="PZ377_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDM6358050.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            2566..2985
                     /locus_tag="PZ377_001170"
     CDS             2566..2985
                     /locus_tag="PZ377_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDM6358051.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            3012..3122
                     /locus_tag="PZ377_001171"
     CDS             3012..3122
                     /locus_tag="PZ377_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358052.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(3192..6962)
                     /locus_tag="PZ377_001172"
     CDS             complement(3192..6962)
                     /locus_tag="PZ377_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDM6358053.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(7190..7624)
                     /locus_tag="PZ377_001173"
     CDS             complement(7190..7624)
                     /locus_tag="PZ377_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6358054.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(7779..8249)
                     /locus_tag="PZ377_001174"
     CDS             complement(7779..8249)
                     /locus_tag="PZ377_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDM6358055.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(8297..9514)
                     /locus_tag="PZ377_001175"
     CDS             complement(8297..9514)
                     /locus_tag="PZ377_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDM6358056.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
     gene            9965..11158
                     /gene="fmtA"
                     /locus_tag="PZ377_001176"
     CDS             9965..11158
                     /gene="fmtA"
                     /locus_tag="PZ377_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDM6358057.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(11695..11973)
                     /gene="qoxD"
                     /locus_tag="PZ377_001177"
     CDS             complement(11695..11973)
                     /gene="qoxD"
                     /locus_tag="PZ377_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDM6358058.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(11982..12587)
                     /gene="qoxC"
                     /locus_tag="PZ377_001178"
     CDS             complement(11982..12587)
                     /gene="qoxC"
                     /locus_tag="PZ377_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDM6358059.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(12577..14565)
                     /gene="qoxB"
                     /locus_tag="PZ377_001179"
     CDS             complement(12577..14565)
                     /gene="qoxB"
                     /locus_tag="PZ377_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDM6358060.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(14565..15665)
                     /gene="qoxA"
                     /locus_tag="PZ377_001180"
     CDS             complement(14565..15665)
                     /gene="qoxA"
                     /locus_tag="PZ377_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDM6358061.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            15967..16089
                     /locus_tag="PZ377_001181"
     CDS             15967..16089
                     /locus_tag="PZ377_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358062.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
BASE COUNT         5383 a         2527 c         2679 g         5626 t
ORIGIN      
        1 aactgaacgt actatgaatt tagttcacga cgtccagttc aacatatcat ttttatttag
       61 tatttaaata tttagcaagt ctcttcatac cttcttttaa aacatccatt tcataagcat
      121 aggaaatcct tacaaatcct ttaccgaatt ctgtaaagga tgagcctgga acgattgcta
      181 aatgtgttga ttcaagtaaa tcgacacaaa attcgaaatc gtcatcggtg atatgtttaa
      241 tacttgggaa aatataaaac gcgccttcag gttgagcggt aatctcaaaa cctaatttag
      301 ttaattcaga tactaaataa tttcttcgtt ctacataagc ttcgttcata tatttaggag
      361 cttctaatcc ttcgttaagt gctgttatac atgctatttg agctggaaca ttggcacaaa
      421 tacaattata ggcgtgcata aatgttaatt tatcaatcaa atattgaggt cctaatagaa
      481 aaccaattct tattccggtt gctgagtgtg atttacttaa accaccaatt aatattaatt
      541 gatcacgaat gtcttcaaat tcagcgaagg atacatgttt accactaaat gtattttcag
      601 cataaatctc atcgctaata ataaataacg gatatttttt taatacattt acgatattta
      661 aaacttcatt tctttttaaa actacgccag ttggattagt cggataattt aacaagacag
      721 cttttgtctt tggagaaata tgactttcta atgcatcagg tgtaatttta aattgtgttg
      781 ctgttgtatc aatataaatt ggtttaccac ctagtacttc gatgagtggt atgtagcctg
      841 cataaattgg tcccggtatt ataatttcat ctccaggctc tatgatagaa cgtaacgttg
      901 tgtctattgc ttcacttgct ccatttgtca caataatttc ttcaggatca taggaaaaat
      961 gataacgatt tttgaagtat tgactaattg cttcgcgagt ttctaataac cctttattgt
     1021 gagagtatga tgtcttgtca ttgttaatag catcaatata tgcctttttc acaacatcag
     1081 gcatcgggaa gtctggttgg ccaatagtta aattaacaca atcatccaaa ttattcatac
     1141 gatttgaaaa ttggcgaata cttggtgctc ttaaatattt agaattagaa tttaaagaaa
     1201 gtttcatttg tgacacctca aatataaatc aaatattgtc taaaaattta gaaattaata
     1261 atatcataac atacttttaa agcaaaaaag ggtaaattac ttaagcttta tttgaaaaac
     1321 gaaaaatgtc taaatacact gtagtaacta cttttcaaaa tgaataaagt ggttacagtt
     1381 aatgtactta gacagtataa aattatgaat ctttaaactg tgatggtctt tgtaaattaa
     1441 ttattggatt tgtccattta caaacaaagt ttgtagataa tacatatacg atgattacag
     1501 atatacttat taaataaaga taagtcatta atgatatagg attatcgaat gggtaccatt
     1561 caaatcctct aacaatgcca ataattaaac catgtaataa atatacgtat agcgtacgac
     1621 taccaatata agtatataat tttttctttg ttgacattaa atttagaaac gcagtcattg
     1681 cgattaatat aattccatat aatataagtc gtttaaaagg actgaatata ctctgtcctt
     1741 cattttcaag tgaagtatat ggtgaacttc ccaataacca atctgcattg ataggatgaa
     1801 tcacgtaaac gataaaaaac aaaataaagg taatgataga tactggtatt agttttttat
     1861 ttttaaaaat agccgtatgt tttttggtga aaatgtaacc tagataaaat attgggaaaa
     1921 atacgattgt ccttgaaatg cttaagtagc tatcgatgtt atctgaaaaa cctgctccaa
     1981 tagatataat aattgaaact gatagcactt tatatggatt aaatcttcta actattacta
     2041 aaatgacatg aaagaaaaat agcgtgatca aaaaccataa cgcaaatact gggttaaaag
     2101 gatcaagttg taattcgtca cttttacctg ttaagaaata ataaattgaa aagaatgcaa
     2161 aaaatatcat ataaggtact atcaaacgtt ttgaaatttt ttctaaatag tatggtttat
     2221 caatattttt cgcgaaataa ccagatataa acaaaaatgt tggcatatgg aaactataaa
     2281 taactaggta taatgctgat aaatatttat cgccactagt gtagggttgt aacatatgtc
     2341 caaatacgac taataatatt aaaattgctc ttgcgttgtc aaaaaaataa tctctctctt
     2401 ttaatgaagt catattttat ctcctttatg tccataagac tcttactaat ttattatatt
     2461 ttgtattagt tatgcaacaa atttgtctat tgtgtcgaat agtattgtaa aaattataaa
     2521 aaaagcatat aatattataa ataatatgta gtggagtaga acaacatgta taaacaactt
     2581 gaaaaactta ttacactgac taacaatgac ttaaacttag tgaatagaag atttggacaa
     2641 cgcacggata tcaaatctga acagctagaa cttctccgta ttttatttaa ttacgatcgc
     2701 ttatcacagt atgatttgac gatgaagatt agcagggaac aatctatagt ttcaaggtgg
     2761 attaagaaat tagttttgaa agggtacatc acaagtcaac aatctagcga agatttaaga
     2821 tgtaaagaat taattttgac tgatcaagca cgtacattaa tttcacaaat aaataatgca
     2881 cgttgcgaat tgattgaagc aagatgtcaa tgtttatcgg aagtcgaatt agacaattta
     2941 aatcaattac ttgataagtt aaatcaacga cgcatatcgt tgtaacacga caatagcaaa
     3001 cacataattt agtgtcgttg tgcacacaac aaaaattgaa tcatgaatta caagcaaaag
     3061 tagcggtgat tgttaaaatt gatggtaaac aatcaccgct atttttgctt gtgtatgtat
     3121 aaaaaaggga tcaaaggtca tcccccatga ttgatagtgg gggatgactt ttgatcctat
     3181 gttcatgttg cttatttata ttgtgggatg tcgaagtatt tgccgacttc gccaatttta
     3241 tcatagtagc ctttgatgat tttagcattg atgttagccc aatctacatc tgtagcatat
     3301 tggtgtgttc ctggatgtgc aggattccat ctcattttgt aaagtgtatt ttgaccagct
     3361 tttacatatg agttgccgat gaatttagca ccaccaacga ttgcttttga tactgtgtcc
     3421 caaccagctt gtttagcata tttaatacct tcacgtaaag gatcgttatc atatgcagca
     3481 ataccaaata cgttatggta tttcgtgttt gagttagtta caactttgtt gttcactaca
     3541 tctgcacctt tcgctaattg agaagtaccg ttacctgttt ctaatagggc atgtgagata
     3601 agataaactt cattaatgcc atacatttga gcagctttgt taaatgcagc accttggttt
     3661 tctaatacac ctttaccttt taagaattga ttaattttat caatagaaat attttgtggt
     3721 tggtctaagc gtaagaattg atattttaat gctggatctt gagctaaacg cttcgtatcc
     3781 attgcatgct taacatcatt aaagttagca tctgtccact tacctggtac acgttgtact
     3841 tgtggtttat attgtaaacc agcttgtatt tgagcaactt ggtttaatgt catacctgtt
     3901 tgattatact taattaattc tttagctaaa tcagttgatt taatccatgc taatttaccg
     3961 ttagataatt taccatagta ccaagtttgt ccattaatga cttgttcttt aacaactgcg
     4021 aatggttgtt cattaaatgc ttttaatgag tatttagctg tatcagaatt tggtgttaca
     4081 tagtaataac cattaccatt tttaattaca taagtgtagt tataatcttt ggcagctgat
     4141 gtagttggtt tcacagcggt tggtgcagtt aaatcttttg catttaccca accagtgcgg
     4201 ttattaatag taccgtataa ataaacatct ttgcctacag atacttgttt cgttgcatta
     4261 aatgtacctt gagcaatgtt attgcctgtt aaaatgactt ggtttttagt accccaagga
     4321 accattgata agccgttatt tgatttatta acagtatatt tttgagtcgt tttaacttct
     4381 ttgcctaagt tttgaacatt taagtctttt acattgaacc aacctaatgg gatgttatgg
     4441 cttgtattgt ttaataatac atacgtttca ttaccatgag cacgctcttt tgttacatag
     4501 aacgtacggt ctgcatattt cgcaccgttt ttcgctgttt tttcataaac agaagcacga
     4561 ataccagtgt tgtttggttt aacttgagca atcttgctaa ctgtttgagt cgtttgtggt
     4621 ttagtaacag tataagcttt tacagctgtt tttggttgtg ctactgcttt tttaggtgca
     4681 gcaggtacag ctaaatatgc tttacttacc caaccagatt taccatttac agttccaaat
     4741 aaatagatag atttatcaat ttgttgttgc ttagtcgctt taaaagtttg gttacctgta
     4801 ccagaaactg caccagcttc ttgtttataa gtgccccaag gtactgaata taatttagtg
     4861 cctggtttta ctgtatatgt ttgcattaca tttacaggtg attttgcatt gttataaata
     4921 acgtcacctt gtttaaccca accaattaaa gttggactat tgtaatcttt aactaagtag
     4981 aatttgtttc cacctaaact tgcttctttt gttacagcaa atgttttttg aacttctttc
     5041 gttggcttac cagttttgtc ataaactgta gtgaataagc cattgttttt agcattaatt
     5101 tgagcaacac cgtttaatga tgaaactgtt aatttattat ttgttgtagg tgttgatggc
     5161 ttaggtgttg gtgtaggcgt aggtttagca gtatcaacta aatatgcttt acttacccaa
     5221 ccagatttac cattcacaga gccatataaa taaattgatt tatcaatttg ttgttgcttt
     5281 gaagccttaa atgtttggtt tccagagcca gacacactac cagcaacttg tttagatgta
     5341 ccccaaggta ctgtataaag tttcgtacca ggtttgattg aatatgattg atttacattt
     5401 acaggtgatt tagctgtgtt gtaaaccaca tcgccttctt taacccaacc aaatttatta
     5461 ccagaattgt aatcttgaac aagatagaat ttttgattac ctaatgtagc tgttttagat
     5521 acagcaaatg ttttttgaac ttcattagtt gctttaccag ttttgtcgta tacagtagta
     5581 tataaaccac tatttgttgg tttgatttgt gcgacaccat tgtttgcagc aactgttaat
     5641 ttaccagttg atggtttcga cggtgttgtt ggttttgatg gtgtagtagg ggtagttgta
     5701 gattgcgtac cccatggcgc cactttaccc atttttatta aatatttttc attaattaag
     5761 tcatataatt gatcataact ataattatga cttcttaaat atccatgtgg atcggcatgg
     5821 tcagtaccac ctaaatattt acttacagcg tagtgagtcc atactgtacc atttccatca
     5881 tactcagcac tgtctggttt taaaccataa tattgtaatt gtgtagctgc atagtcagca
     5941 tagttattca ttgaacgtgc aaatgaagca tagtcgtgtg tgtgtacgat ttcaacattg
     6001 atgaatctag ggttaccgac tgcaccgaca ccccaagata agtaatccgt tggtgctgtt
     6061 tcgattatac gatccccatc aacaaatgca tgtacgaatg cgttttgata gttatttttc
     6121 atataactaa tttcaccatt tatcgtcgaa cgatcattag ctgtatcatg aactacgata
     6181 ccttcaggac gacctacgcc gttacggtat gcgtatttag ggaagtaaga tgtataatct
     6241 tcttcaattt taggtgcttt taagttattt ttacgaatgt aatcgttaat tgaagagttt
     6301 acttgtggtt tatattttgg caaactcgtt tttggtgttg cagcaactga tcttggttgt
     6361 gctgaagcgc taaaagtagt tactttaggt gtcgcttcag ttttagcttt aggtgctgat
     6421 gtagttgcag ctttaggtgc tgcggtttta tattgcgttt caagagctgc aggtttagca
     6481 gctgatttaa ttaattctgg attaatttga ttttctgaat tatcatcttc atcatcaact
     6541 aaactataac cagcatttgt aacattagtg ttagttttag gtgctgtagt gcttgttgac
     6601 tttgcaacag gctgcgtatt atttgtagtc gctgattgat tagcacgagt gtcaccattt
     6661 acttgtgcag tatcaacttt ttgacttact tgagcattgc ctgttttgtt atttgctgtt
     6721 tttggttgga caatagcagg gtcttgatat acttgagtgc cagaaatgtt ttgcgttgga
     6781 ttttttacct cagcttttgc ttgttcagta gttgctttaa ctttattact atctaaaacg
     6841 tttttattag tagtttgatc ttgtgtcgtc tcagctgctt gaacttgatg tgcagtgact
     6901 gctgaaccta caagcgttaa tgcaaccatt gatggtagtt tgtaattgaa ttttttcgcc
     6961 attctattta ttactcctaa catttattaa ttattactaa cattatagta cctgttttat
     7021 atacctgtgc gtatttaacc aatttgtaat atactcttaa tttacatttt cactgtaaaa
     7081 aaatgaaaaa aattttgaat ctcaatggtg acgtgacaaa tcaaatagaa ttgatgtcaa
     7141 tttaagaatt ggtttattac aaatgtttct aaaatagata caactttttt taattaagcg
     7201 aggttaactt ttttgtcatt tcaatatgct cgatgccttc ctcaagaaat atattacctc
     7261 tcattttaaa gtttaaactt tcataaaatg ggatagcatg acattgggca ttcatagtag
     7321 ctacgtaaaa accttcatct ttagctaatg attctacagc ttgcataagc attctaccca
     7381 ttccttgtcc acgatgtgat ttcatcacag ctactcgttc tattttgaca gttgtttcat
     7441 taatagggcg tattcgagca gtggcaactg gctgtccatt atcatatcca atgaggtgaa
     7501 tagattcaga ttcatattca tcaatttcac tttcctcagg gacgccttgt tcttctacaa
     7561 acactttctt tcttatatag aagcaatctt ctaacatctt ttgattgttt acttttgaaa
     7621 acataaaatt cctccacatc ctaacaaaat catttacatt taatttacta ataataacat
     7681 agaataacga ttgttatttt cctcaaaaat aattagacat aaaaaagcta taaagatgat
     7741 tgttgtgaat catctttata gctaattaat tattaatgtt atagtgcttc ttttctaata
     7801 attgctgtat attgccaaaa gattcgcatt tgagcaatat tccaattatt cattcccatt
     7861 gtaaatcctg aaggcttaaa tatattcaca tcagttactt ctaaagctgc tgcaactaaa
     7921 tattgtaatg gaatgctaag tgatttcatt gtttcggtaa tgccattttc atcatatacc
     7981 catgaaaatg gtaactctga ttcgaaaaca tcaacactat tgaaaatttc aggtagggct
     8041 aaaatataac atgcaccatc tacaatagga ttgtcgatac tatttttgta ataaattagg
     8101 agtgcttcat agttttctcg atgctcatga ttgacgtaaa aaaattcact tctaaaatga
     8161 gccatatctt ggctatgaat gatttgttgt tctaacatat taaacatgcc atttatattt
     8221 gcaatctttt cgtatgtttt tctactcatg tatgatgctc ctttcataaa tagtaaatgt
     8281 taatcgtcat ataatattaa tttacaacac cattttggtt atttgaagct tgtggcgctt
     8341 gttgtgtgcc accttgattt tgatttgagt tttgatctgt agcaggttgt tgttgattgc
     8401 tggaatcact gttattagtt gaatcactgt tctcgttaga tgtcttatct ttatctgtcg
     8461 tatcattttg ttcttttctc aataaactat tatctaaagg cgttaatggt attaatgaac
     8521 cataatgatt aatgacacgt tgatctaaga aatcattttt atcattaata ggtgataatt
     8581 ctaagtcttt acgaagtaag tttgcatatt tttgaatgct ttcaacactt ggatgataat
     8641 agtaaatacc atttaacata tcatctttac cttttaattg cgcagtttta atttcaacat
     8701 catttgttaa gtacattttt gctaaagctt taatttcaga gttagttaaa ttatgctttg
     8761 catttttacc tacaatttga atcacgttat caagtttatc aatagaatca acttcctgtg
     8821 ctttttggaa taaaatctta attaattcca tttgacgttg tccacgtttt aagtctgaat
     8881 catgatgtct agttctagca actgctaaag cctcatcacc atttaatttt tggtaccctt
     8941 ttttaatttt aatcttacca gtatcatctg tgttaggttc atttaagtcg tatggcacat
     9001 catagtatat gccaccaagc tcatttacag cctcgacaaa tgctttcata ttgactctca
     9061 cataataatc aacaggtaca ttcatggtag cttctaccga atccattgcg gcaattggac
     9121 caccatatgc atgtgcatgg gtaatcttat cgtaatagcc aactttagga atgtagctga
     9181 tagtatcacg tggaatacta agcattctaa tttgatgttt tgattgatta aaagtagtta
     9241 aaatcatagc gtctgatcta gagtgttcag catcctgtcc tttttttctt cttccatcgt
     9301 tatcatcgat acctaagaaa agaatagaga taggttgttc ttcgggattg actttattat
     9361 ctcttaagtt ggattgacga ttagcatttt tgctgtcttg agaagattcg aatgcatctt
     9421 gggacgtttt aaaaagtaac gtagcgaaga ctattggaac aacaatgaga accaatgcta
     9481 gaaggatcaa aaagtatttt aaaaatttat tcatgattga tgctcctata ttaaattttt
     9541 gttaaatcat aatcataatt ttaaaatata attagttgaa cgatatttat acaacttcat
     9601 tactaataca ttatagcctt aaattgtagt actttatagt aaaaaataca aatgttatgt
     9661 caaagttcct tgtaatttga atgttttgtt aaaatcaaaa agcattttta aaaatataat
     9721 gcgactttag tatgatgtct taaaaaattt aaaggcgtaa tataaattcg agtgatttat
     9781 acaaagtgat aaggtataat taattttgtt gtatagataa catattagaa aaacttatta
     9841 ctatgtagcg taaagcataa atgaatgata agttaatttt tgtaagttgt attattaggc
     9901 atctaggttc aaatttttaa cgatttaaat atataaaatg aatacattgt gaggcaatta
     9961 tcaaatgaaa tttaataaag taaaactagt tatacatgcg tgtgtactat tatttatcat
    10021 tatttctata gcgttaattt ttcatcgatt acagacgaag acacattcta tagacccaat
    10081 acataaggaa acaaaattat cagacaatga aaaatattta gtggatcgta ataaggaaaa
    10141 ggttgcgccg tctaaactaa aagaggtata taatagcaag gatcctaaat ataagaaaat
    10201 tgacaagtat ttacaaagtt cattatttaa cggttcagta gctatatatg aaaatggcaa
    10261 attgaaaatg agtaaaggtt atggatatca agattttgaa aaaggtatta aaaacacacc
    10321 gaatacgatg tttttaatag gttcagctca aaaattttca acagggttac tgttaaaaca
    10381 gttagaagaa gaacataaaa taaatatcaa tgatccagta agtaaatacc ttccatggtt
    10441 taaaacatct aagcctatcc cattgaaaga tttaatgttg catcaaagtg gattatataa
    10501 atataaatcc tcaaaagatt ataaaaattt agatcaagca gttaaagcga ttcaaaaacg
    10561 tggtattgat cctaagaaat acaaaaagca tatgtataac gatgggaatt atttagtact
    10621 tgcgaaagta attgaagaag ttacaggtaa atcttatgct gagaattatt atacaaaaat
    10681 aggagatcct ttaaaacttc agcacacagc attttatgat gaacaacctt ttaaaaaata
    10741 tctagcaaaa ggttatgctt ataatagtac aggactttca ttcttaagac ctaatatttt
    10801 ggaccaatac tatggtgcag gtaatttata tatgacacca acagatatgg gtaaattaat
    10861 tactcaaata caacaatata aattattcag tcctaaaata accaatccat tattacatga
    10921 gtttggtacg aaaaagtatc cagatgaata tcgatatggt ttctatgcta agccaacatt
    10981 gaatagactt aacgggggat tctttggaca agtctttact gtttactata atgataagta
    11041 tgtagttgta cttgcattaa atgtaaaagg aaacaatgaa gttcgaatca aacatattta
    11101 taatgatatt ttaaaacaaa ataaacctta caatacgaag ggtgttattg ttcaataatt
    11161 aatatagaag atataacatg tatatggcat taaggcatcg accttatctg accagtatac
    11221 gagttatatc ttctttttta tagtggtaaa aagtttaaag tataaggttg aagaaggatg
    11281 agtttaaaaa tatgtgttaa ctgataaaag gggaaatcat ttggtgagtt ggcatcagac
    11341 taaaatgaat gaagacgaat acgttggtcc atgcgtggtg tgaatgtttt ctaaatctaa
    11401 ctggaaatga taatttaata atgtacactt ttgattgttt aaacatgtac aatttttaaa
    11461 aacctaggga taattttatt gacgtgaaca acattgagat tatctttaga cataaatatg
    11521 gattttgaag ttactatatt tattagttgt ggttgttaat gtattaattg aattgattat
    11581 aatgattgtc ttataagata tagaagggga cttaaccatt taaaaattga aggaaaataa
    11641 aaaccagcta acaaaagtgt tagctggtta aactttacat tattaaaatg ttgtttataa
    11701 gtgtgaagag tgaccgcctt gcataaccca atatgttccg acaacgaaac aaagtgtaat
    11761 tacaagagca aagataactt tgaatgtttg taaacgtcca tctttacctt cagttaaatg
    11821 catgaacatt aataattgaa gtcctgcttg gacgaatgca aagccaaaga taattgtcaa
    11881 cttcgcgtgg aatgttaatg acgtgtatag tgttacgtaa actgctaaaa gcgttaatac
    11941 gatagatgcg ataaatccta cagtatgttt cattattgta ctcatccgct atacaccatc
    12001 cctatcatat atacggcagt aaagatgaaa acccaaacaa catctaagaa gtgccagtat
    12061 aaacttacta taaataattt tggcgcatta tatttgtcta atccgcgtcg ttggatttgg
    12121 attaataaac aaatggccca aacgatacct agcgatacgt gacaaccatg cgttcctaat
    12181 aggataaaga aactagacca gtaagaacca attgttgggt taacgccttc tgatgcatag
    12241 tgtgcgaatt cataaatttc gaatccaaca aagactaaac ctaaaagtaa cgtaatgatc
    12301 atccaaaaca tcattaactt ttgtttttct tggcgcatgt agtaaatagc aataccacat
    12361 gtgtaagaac tgaataataa tgcaaacgtc attattaaaa caagaggcaa ttcaaataac
    12421 tcagtagtca ttttacctgc ataatcgcca ccatgttgca aagttaatag tgtcgcaaat
    12481 agggtaccga ataacgcaaa ttcggctgta atgaaaatcc aaaagccaag cttatttaat
    12541 tcgccttcat gtgtgcgtga atcaatagtg tttgtatcat gactcatgac ttacagcctc
    12601 cctttcttta atacgcgctt ctcttaatct agcttcagtt tctgcaactt cagcagcagg
    12661 gatatgatat ccgtgatcga tttggaaact gcgataaatc atagtaccaa aaataccgaa
    12721 taaacaaatt aatgctggaa ttacagtttc gaaaattaag aagaaaccgc cgatagtcat
    12781 aaagatacca atccagaatc caacaggagt attgtttggc atatgaatgt ctttgtaatt
    12841 atggttgtct aagtaatgac gaccatgttc tttcatatca acaaatgtgt cgtagtcatt
    12901 ccaatctggt gtaatggcaa agttgtattt aggtggaatt gctgatgcag tagtccactc
    12961 tagagtacga ccaaggccat cccagttatc tccagttgct tcacgtggag atttgaagtg
    13021 actgtatacg atactaacaa caaggaataa gaatccgatt gccattaata atgcaccgat
    13081 agttgaaatt aagtttaata agaaccaacc atctgatggc atataagtgt ataaacgacg
    13141 tggcatacca tctaatccaa gaatgaattg tggtaagaaa caaacgttaa atccgatcat
    13201 gaagaaccag aagcaccatt tgtttaatgt ttcgtttaat ttgtaaccca tcatctttgg
    13261 ataccagaag attaaaccag ctaagcaggc aaatacaaca ccagtaacca atgtatagtg
    13321 gaagtgagct actaagaagt acgtattgtg atattgatag tcagctgatg ccattgctaa
    13381 cattacaccc gtaacaccac ctaaaaggaa gttagggata aatgctaatg agaatagcat
    13441 tggtgactca aatgtaatac gtcctttata taatgttaat aaccagttaa acaatttcac
    13501 accagttgga ataccaatca gcattgttga aattgagaag aatgagttga ttaacgcacc
    13561 attacccatt gtgaagaaat ggtgaaccca aactaagaaa ctaaggaacg cgatgccggc
    13621 agttgcccat accatacttt gatgtccgaa taaacgctta cgagcgaatg tcgggataat
    13681 ttctgagtaa ataccaaatg ctggaaggat aacgatataa acttcagggt gcccccatac
    13741 ccagaagaag ttagcccaaa gcattggcat accgccatgt gcaactgtga agaatgctgt
    13801 gtcaaatatt ctatcagttg tcattaatgc taacgctact gttaaaggag ggaaagcaag
    13861 aataacaatt aatgtagtaa taaatgttgt tactgtaaac attggcattt gcataaactt
    13921 catagttggt gttttacatc ttaaaattgt tacaaagaag ttgatacctg tagctaaggt
    13981 accaagccct gaaatttgta tagctattaa gtaatagtta acacccggac caggactgaa
    14041 ttcacctgct agtggcgcat agtttgtcca accagctgct ggtgaaccac caataataaa
    14101 tgacaggttg aataaaatca tacctgcaaa gaatagccag aaacttacgt tgtttaatac
    14161 tgggaatgca acatcacgtg ctccaatttg taatggaaca acgatattcc ataaaccaaa
    14221 gataaatggc attgccatga agataatcat gattacacca tgtgtactaa aaatttcgtt
    14281 atagtggtta gattctaaaa atttgttatc aggtactgtt aattgcgcac gaataagtaa
    14341 cgcatcaata ccaccacgga cgaacattaa tacggcacag attaaataca taataccgat
    14401 tttcttatgg tctacagatg tgaaccattc tttgtaaaga tatttccata atttaaagta
    14461 agtaattact gcgattaaac caataactaa gaatggggca ccaatttgtg ccattgtaat
    14521 catccagtta cctttaacta gtaattgatc ccatggaaaa ttcattaatg tccacctcca
    14581 tgatcatcat tgtcttgatc ttgcgcatct tttgaaattt tcttcatttc tttcgcattt
    14641 ttcgattcat ctttcttgaa ctcattgtta tatggttcgt catttccaag aatcatcaac
    14701 ttcataccat gtcgtttata gttcgcattt gtaatttgag ctttacgagc aggtattaat
    14761 ggtttgtctg atacatcttt aaacatattt tcttcactag tgaagtttgg atcttttaat
    14821 tcgaaattga aacgtttata tgcatagaag atgtattctg gatcggctgc tggatcaaca
    14881 aacgccatat gtgtaccatt aaattctaaa gctttattag gtgtgcttgg taataattgt
    14941 ttatcaaatg tatcttgatc taacgttttc ttacctttaa cttctttcac ccatttgtcg
    15001 tagtcttttt gactaacggc atttacttta aatgtttgac gtgagaatcc ttcaccattg
    15061 aagttagagt tacgacctct gaacgtacca gtttgagatg cttctaacgt ccaattcatt
    15121 gtcatgccag tcatggcata tttttgacca cctaattgtg gaatccagaa acttgtcatt
    15181 gtatccatag cttgaagctt aaatacaaca ggacgatctt tagggattgt taatgtatta
    15241 acagtctcta tatgttcatc tggataagca aagaaccatt tgtatcctgc acttactgca
    15301 tatacaacca ttggatcttt ctcactcttc ggtggttttt cgtaatcgta taaagtttta
    15361 actgtaggaa tagctaaagc agcaacgatt atgataggta ttacaaacca tattgtttca
    15421 atgatggcat tatggtgcat cttaccagat tcggcattct tattataact atacttgtaa
    15481 ataaaaatgg cgaacatgcc aagtacaacg aaacaaataa caagcatgaa gacgattgaa
    15541 taaagaatca agaacttctg actacttgct actggccctt ttgcgttgaa aatttctata
    15601 tttgaacaac cactaagtaa aattagtgtg ccaaataata gaagcaaaga cttaaatttt
    15661 gacacttttt tgacctccta atactacaaa tgtagggctt aacattaatt ttaagttatt
    15721 acacaatatt tacaagggct tatgggaaaa aaattaataa aattgtatca aaaatgttga
    15781 taaatcaagg tgtgacgtgg gttcacacat ttgttaaaat tatgtgtaca ttttgtgact
    15841 aatagcgttt ttgaaaaaat tgcttcatat ttaaaaggaa agttgagaat agctttcaat
    15901 taaaggttaa ttgaatgaaa ataaaaaaag tttgaaattg tctaataaat tgaaaatgat
    15961 ggctagttga ttgaaaggaa gcgcaaacat tttaataagg aatttgccaa aagtaattta
    16021 tcgttaaaca atgaaaagtg gaaatttgtt tgttcaaaat caaaacattt ttttaaaaag
    16081 agcagttaag ttttaataat ttgataggta gtcctttggt atacagaaaa aatacaatat
    16141 ttgggttaaa gttgcagatg aagttagaat aacaaataaa atggtagcaa aacatgttcg
    16201 tttgctacca tttta
//
LOCUS       DANRDI010000046        16113 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.046, whole genome shotgun sequence.
ACCESSION   DANRDI010000046 DANRDI010000000
VERSION     DANRDI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.046"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            363..758
                     /locus_tag="PZ377_001182"
     CDS             363..758
                     /locus_tag="PZ377_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358063.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            954..2339
                     /gene="fumC"
                     /locus_tag="PZ377_001183"
     CDS             954..2339
                     /gene="fumC"
                     /locus_tag="PZ377_001183"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDM6358064.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(2802..3623)
                     /locus_tag="PZ377_001184"
     CDS             complement(2802..3623)
                     /locus_tag="PZ377_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDM6358065.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            3786..4898
                     /locus_tag="PZ377_001185"
     CDS             3786..4898
                     /locus_tag="PZ377_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDM6358066.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            4920..5543
                     /locus_tag="PZ377_001186"
     CDS             4920..5543
                     /locus_tag="PZ377_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6358067.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            5899..6363
                     /locus_tag="PZ377_001187"
     CDS             5899..6363
                     /locus_tag="PZ377_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358068.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            6542..7666
                     /locus_tag="PZ377_001188"
     CDS             6542..7666
                     /locus_tag="PZ377_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDM6358069.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            7735..8079
                     /locus_tag="PZ377_001189"
     CDS             7735..8079
                     /locus_tag="PZ377_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDM6358070.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            8462..8560
                     /locus_tag="PZ377_001190"
     CDS             8462..8560
                     /locus_tag="PZ377_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358071.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            8851..10047
                     /locus_tag="PZ377_001191"
     CDS             8851..10047
                     /locus_tag="PZ377_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDM6358072.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            10037..12973
                     /locus_tag="PZ377_001192"
     CDS             10037..12973
                     /locus_tag="PZ377_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDM6358073.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            12970..13911
                     /gene="yhaM"
                     /locus_tag="PZ377_001193"
     CDS             12970..13911
                     /gene="yhaM"
                     /locus_tag="PZ377_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDM6358074.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(14032..14994)
                     /locus_tag="PZ377_001194"
     CDS             complement(14032..14994)
                     /locus_tag="PZ377_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDM6358075.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(15199..15756)
                     /locus_tag="PZ377_001195"
     CDS             complement(15199..15756)
                     /locus_tag="PZ377_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDM6358076.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
BASE COUNT         5975 a         2271 c         2727 g         5140 t
ORIGIN      
        1 aatttgcaat gtctgtagaa attgggaatc caattccact atgttggagc tccaccaact
       61 tgcatgaaat agaagctgac attccgaagc ctctatgttg tggactgcag tcaactactg
      121 ccaatataac tttgtaaagc ataaaacatt gatttgtgta ccagacatag taatatatca
      181 aaagttcaat tttaaatatt aaaacaccat ttaaacggaa ttgataatta attactccag
      241 acataattga aagaagtcat ctatcagtaa tatttttaat gaaatcatta aaccgcgttt
      301 cttaatatgc caagattatg atatgctatt gaagaatgaa attagaggag atttatatat
      361 ttatgttagg aaaatactgg attcaattat tgatagcaac agttattata agtttaatat
      421 caattaaagc ttttccattg gcaattgggg cattatattt acctgttatt ttcaaagtaa
      481 taaaattaca actcaattta tcaaaaggat taattgatga tgttaatgcg caaacattca
      541 ttaaaagtaa tcaatcaggt attgtaatca gtgtaatttg ttgtttatta ataacagcaa
      601 tattgtatta tacacttgat gggttttatg cgagtttaac aggagtttta ggtacgcttg
      661 ttgctctaaa tccatatacg acaattgtta gtgctgtatt atatatactt acagctattg
      721 ctacggttga agctactaaa acaaagtatc gtaattaaac ttagttcaat aataactaaa
      781 taatgataga taacacacta atcgtatgac cgtatcactg taataaaggg tgaacacgtg
      841 cgattagtgt atttttattt caaattatga aaacgtttta atcataaatt gctcaattaa
      901 attatttaat gatatactac agttaaataa acagtgataa ggggagaaat tgaatgtcag
      961 taagaattga acatgatact tttggagaaa tagaagtacc tgcagataaa tattggggtg
     1021 ctcaaacaga aagaagtaaa cgtaatttcc cagttggtaa agagcgtatg ccaatcgaag
     1081 tagtttatgg ttttgcacaa ctaaagcgtg cagcagcaat agctaatttt gatttaggaa
     1141 aattaagcga ggcaaagaaa gatgccattg tatacgcatg tgatcaaatt ttatcaggtg
     1201 aattagatga acacttccca ctagttgtat ggcaaacagg aagcggtaca caaagtaata
     1261 tgaatgtgaa cgaagtagta agttatgttg ctaatatgta tttaaaagat catcaaagtg
     1321 atgaaagtat ccacccaaat gatgatgtaa ataaatctca aagttcgaat gatacattcc
     1381 caactgctat gcacgttgca ttatatcaag aggttgaaac aaaattagaa cctgcattaa
     1441 aacttttaag aaatactttg aaagaaaaag aagataaatt tgattcaatt attaaaattg
     1501 gtcgtacaca tttacaagat gcaacgccga tcaaactagg acaagagatt agtggctggc
     1561 gttatatgct tgaccgttgt gaaacaatgt tatctgaatc taagaagcac attttaaatc
     1621 ttgccatcgg tggtacggct gttggtactg gtattaatgc gcatcctgaa tttggtgata
     1681 aagtggcaca ttatatttca gaaaatacgg gttatccatt tgtatcttct gaaaataaat
     1741 tccacgcact tacagcgcat gatgaagttg ttcaattgca tggaacattg aaggcattag
     1801 caggagactt aatgaaaatt gctaatgatg tgagatggtt ggcttcaggg ccacgagctg
     1861 gtttggcaga aatttctatc cctgaaaatg aaccaggttc atcaattatg cctggtaaag
     1921 ttaatcctac acaatgtgaa atgttaacaa tggttgcagt ccaagtaatg ggtaatgata
     1981 cagttgttgg cttcgcaagt tcacaaggta actttgaatt gaatgtttat aaaccagtta
     2041 ttatgcataa tacactacaa tcaatttatc ttttagctga tggtatggaa acatttaata
     2101 acaattgtgc agtgggcatt gaaccaatcg aagagaatat tgataattat ttaaatcaat
     2161 cattaatgtt agttactgca ttaaatccac atattggtta tgaaaaagca gctcaaattg
     2221 ctaagaaagc ccataaagaa ggtttaactt taaaagaatc tgcaattcaa actggatatg
     2281 ttacagaaga acaatttgaa gcatggatta aaccagaaga tatggtagat cctcattaat
     2341 taaatattaa tataaaataa agtttgggca ttacaggtag atgtagtgtt caagctttat
     2401 tttttgtcta tgtcaaattg aacttataag tctgatttga tatagagcca tgaaaaggaa
     2461 aaacttttca tggagtccgt tttattatag aacctatttt ttatatgatg aattgttgta
     2521 catgtattgg ccaaacaacc tgtgataaaa atcttatgat gtcatgtatt gtccatagat
     2581 acacgtaacg cagttgaaaa agactacaag tcattatcca aggatgttat aaaatgaata
     2641 attactaaaa tatcaaaata taaaatccta agcatcgtaa aaaacaaaaa aaccgaacat
     2701 ctgaaaaaat taataatttt ccatctatcc gatttaagtt gatacttgat ttagttacaa
     2761 tgcggtattg catgtttaaa tatcatgcaa aactaatcaa attagtcatc caaagaaacg
     2821 gaaatgactt ccttagtcaa gggatgcaca aactctattt tgtaactttc aagttctaat
     2881 tgtcttaacg ttgaatcacc atataaagga tcaccaataa cagggtgacc aatttcagct
     2941 aaatgcacac gaatttgatg agtacgtcca gtatccaact tgatttcaag ttggcacaca
     3001 gcttctttta tcatttttga agttaaaatg tgtgtaattg cacgctgtcc agtaggagac
     3061 acacgtcgtt tattcgaatg gaatttatct ttaccaattg gcatatcaat cgtttgtggt
     3121 tttaaaggta aaagtgcctt aacatttgct ttgtatatcc gcgtaatatc attgtcttct
     3181 aacatgcgat caagaatttt tttcattaaa ggatttttag caacaattaa taatcctact
     3241 gtttcctggt ccagtcgatg aattggttcg acatagtcac tatcaatagt gtaaatcaca
     3301 tgattcatta aagtattgct ttcttttaaa tcattagggt gagtcttaac acctttaggt
     3361 ttcattacga ttgccatatc atcgtcttcg tatttaattt gtgcataacg ataacttgga
     3421 acataattac ttttttcatc gatggttgga acaaatacat cgtcgccagt atgcacttta
     3481 tccattaatc gcgcaggttt atcattaata gtaatatctt tagacatatt taaatgatgt
     3541 aaatcttttt taggtacctt aagttgttgg aaaatatctc gtaaacttaa gtcattaaag
     3601 ttttctggta ttttaaattt cataaaatcc tcctatagtc tttaatatca tatcacaaat
     3661 cagttctccg aaatataact attgagtaat caaggccatt ttatcatgtt tacgtctaag
     3721 atttaaaaat ataataactc aatgtataat agttaataca cgtaaaaatg aattggagcg
     3781 atttgatgga acaaaggacg cgactagctt tattaaaaga gattgccgaa tttttaaacg
     3841 aagaaactga aatgtatagc atgacccaag gtgcgttaaa atatttaatt gagggcagta
     3901 atttcacgac aggctggata tttttcatca atagcgtagg tgaacatgaa cttgtatcac
     3961 atgtggctct accacaatct ttgacagcag accactgtca ctatattaaa gatgggtctt
     4021 gttggtgtgt gaaagcattt aatcaacgta ggctaatgaa agcgtcgaat atcgtcaatt
     4081 gttctcgtat taacttagcc tctaaagcat ttcctagcca aaatgacaat attacacatc
     4141 atgcaacggt accacttaaa tcgggtcaag aacaattcgg cattttaaat gtagcgtctc
     4201 caaatactga aatatatagc gatgaagatt tagagttgct agagtctgtc gcatttcaat
     4261 tagggtcagc catcaaacgt atttatttaa cagaccgtga aaaagaagcg gcaaaaatta
     4321 atgaacgtaa tcgtttagct agagatttac atgattcagt aaatcaaatg ttattttctg
     4381 taaaactaac agcacatgct gcgtatggca tgtcaaacga atccatcgct aaacaagcat
     4441 tcaagacgat tgaggaaacg agtcaaaatg ccgttaatga aatgcgtgca ttgatttggc
     4501 aacttaagcc agttggactt gagcaaggtt taattcatgc tttgacagca tatagtaaat
     4561 taatgcatat acaattaaat gtaaatgtag aaggtttaat cgatttatct aatgaaatcg
     4621 aagaaaacat atacagagca ttacaagagt gtattaataa tgttaagaaa catgctgata
     4681 cgaataaaat ggatcttaca ttaaaacaaa tgaacgatat actatatatt gacgtgattg
     4741 attatggtca aggatttgag atcgataatg ttcagatagc ttcatcacac ggtattaata
     4801 acataaaaca aagagttaaa ttattaagag gtaaagtgac ctttcattct cagccaacaa
     4861 agggtacaca aatacaattc acaattccta taaaatagct tgatttaagg agataaccca
     4921 tgaacaaagt aatattagta gatgaccatt atattgtgcg acaaggattg cgatttttat
     4981 tatccacgat tgaaaacata gaagttttac aagactttgc agatggagaa acatttttag
     5041 aatatttaaa agagcatgag caccctgata ttgtgctatt agatttagtg atgcctggca
     5101 tgaatggtat tgaaattacg gaatatatta aggcacatta tccggatatt aaagttttgg
     5161 tattaacaag ttatgttgat gatgaacatg taatttcagc aatcaataaa ggtgctgatg
     5221 gttatgaaat gaaagacgtt gagcctcagc aattaattga aactattaga cgagttatga
     5281 acggtgaaaa aatgatacat cctaaggcac aagatgtatt cgaaacagtt agccaaaaac
     5341 cacactacac gaataagttg tcaaagagag aaattgaagt gttacgtgaa atggttaaag
     5401 gtaaaacaaa taaagagatt gcagaaactt tatttgtatc tgaaaaaaca attaaaacac
     5461 atgtcagtca tatatttagt aaattacaag ttagcgatcg tacacaagca gcaatttatg
     5521 caatggaaaa taagttgatt tagaatgagc ttttaaatat ttgtcgaaaa atagacaatt
     5581 tttaatctga ttttgatgaa ttttttatag cgatagggtt tattatacag ttttctttgt
     5641 aaatttgata tcattataaa agtgtaagtt ttatcaaatc tagtgattat gcataaaaat
     5701 ttacttcatt tactttcgct tcattataaa gtaaacttaa ctcttgttga cttgatcaaa
     5761 atttaataca aatgtgaaat tgactctttt cattttatta aaaatatatt aagatgtaat
     5821 ctgtgtttca agaaatgtgt attattgcaa tttcttgtgt ccggataaaa ttccccaaac
     5881 agaaaggtag ataacagaat ggatagacag agttttacag atttaattca aacaaaattt
     5941 aaaatggttc gtatagaggc tggttatacg caagatacta tggcgcaaac aattggactt
     6001 tctaaaaaga ctttagtaca aatcgaaaaa gagagagtat taccaaactg gacaacttgt
     6061 atttcaattt gtgcattatt cagagactct gaagtattaa acagtacatt tggctgtgat
     6121 ccattagaaa tcgttcaaac aatttcaaga aatcactgtg catatccaaa ccatgcacca
     6181 acaagtgata tttattggaa taacattgag actcgtaatg ggtacatttt acaaagtaac
     6241 aaagtaagca atatttatcg tgtattaaat ccagataacc aaccaatttt cggtacttct
     6301 aaaatgagag aagctgaaac atactttaac agaaatgcta aagaagaatt agtacatatc
     6361 taggcctata tacttttaat aaataataaa agcgatgtaa cacacctgtt tcgagaggtg
     6421 aggcatcgct ttttgtatgt aaactaataa taaattgagc agaaagtgaa atgattaatg
     6481 aattaaaaaa taaaaatagt ggtataatga ataactatgt tgtcacaaag gagtcaaaat
     6541 tatgaatgca ctatttatta tcatatttat gattgtagta ggggcgataa ttggtggcat
     6601 tactaatgta attgcaatta gaatgctgtt tcaccccttt aaaccatact atatatttaa
     6661 atttagagtc ccatttacac caggtttaat accgaaaaga cgcgaagaaa ttgcaactaa
     6721 aattggccaa gtgattgaag agcatttgct tacagaaact ttaattaatg aaaaattgaa
     6781 aagcgagcaa tcacagcaag caatagaatc tatgattcaa cagcagttac aaaagttgac
     6841 gaaagatcaa ttgtcaataa aacaaattac ttctcaaatc gatattgatt tagaacaagt
     6901 attacaaact aatggaaatc aatatattga atcacaattg aataattatt atacaaagca
     6961 tcaaaaccaa acaatagcat ctttattgcc aaatcaactt gtaacatttt taaatcagca
     7021 tgtagataac gcaacagact tattatgtga tcgtgcaagg aattatttat catctgcaaa
     7081 aggcacacaa gatattaatg atatgttgga tacttttttc aatgagaaag gtaagttaat
     7141 tggtatgttg caaatgttta tgacaaaaga gagtattgca gatcgcattc aacaagaact
     7201 tatacgttta acatctcatc ctaaagcaag aacaattgtg acatcgttaa ttaccaatga
     7261 atatcaaact tttaaagata aaccattgaa tgaactgtta gatgcatcgc agtttaatga
     7321 aatcgctgaa aatctatcag tgtatgtaac aacatatgca agcaaacaag cgaataagcc
     7381 agtggttacg ttaatgcctc aattcgttga ttatttagaa ggtcaactct ctagcaaatt
     7441 agcaaattta atcattgaaa aattatcgat tcacttatct acaattatga aaaaagtgga
     7501 tttacgagga ttaattgaag aacaaattaa tacgtttgac ttagattata ttgaaaaatt
     7561 aattatagaa attgctaaca aagaactaaa gttgattatg tcattaggtt tcattttagg
     7621 tggtattatt ggatttttcc aaggtttggt tgcaatcttt gtctaaaaaa gcgagatgtg
     7681 gtaaagtaat atcgaacgtt ttaataaaca tcacaacaaa ggagtgctat aaccatggca
     7741 gtaaatttat atgattatgc aaatcaatta gaacaagctt taagagaaag cgaagaatac
     7801 aaagcaatca aagaagcatt cgctaatgta aaagctaacg aagaatctaa aaagttattc
     7861 gacgagttcc gtgaaactca aattaacttc caacaaaaac aaatgcaagg tgaagaaatt
     7921 gctgaagaag atttacaaaa agcgcaagaa caagcgcaag caattgaaaa agatgaaaac
     7981 atctctgcat taatgaatgc tgaacaaaaa atgagtcaag tattccaaga aatcaaccaa
     8041 attatcgtta aaccattaga cgaaatttac gctgactaat attaaaataa tatttttcaa
     8101 tcctttaaat agaggaatag actgccgaca gagtccgaga ctttgtgggt agttttttta
     8161 gttttgataa cggaagttag aggctctctg tcaaattggg cagatgcatt cagtaacgga
     8221 agttaggcta gtaaacgtgt cctaacttcc tttttgcatt ttgaagtgta aagttttcaa
     8281 cataatacta ttagttcggt catgtatcgg actgatggaa aagcgtttca cttttaatga
     8341 ctcattaaga acggcctgaa aatgtttggc gtattaagtg caatgatagt tttgacattt
     8401 agtttctaat tggtcattac tgccgagcaa atctagtaga gtaatcatgt aaatctttaa
     8461 tgtgccattt gattcactag cggtgttaat aactacggaa attgcatttc cgactgaaat
     8521 ttttgaaaaa tatcaacgta cgctacaaat aaaattttaa actgttataa atgtgtctca
     8581 atttcatatg ttcatcgacg atatgaagcg tattatggta aaatgaagaa ataataaact
     8641 tgttaataaa taaaacatca cgatttgact aaagcacttt attattgtgt agataatagt
     8701 tttttaacga aataaaaatg gcgactggtt ttaataaatc agctaatgaa tcactacacc
     8761 tataagtatg aatatagtga ttagaatgct ttgtatagtt ggattttgca aaattgatgt
     8821 tagaaccttt aattaaggag atatggatgt atggttaaat ttattcattg ttcggattta
     8881 catttagata gtcctttcaa atctaagagt catataagcc ctaaaatttt tgaggatgtt
     8941 caaaaaagtg cttatgaaag ttttaaaaat attgtagata ttgcattaca acaagatgtt
     9001 gattttgtaa ttatcgcggg tgatttattt gatagtgaaa acagaacatt acgtgctgaa
     9061 attttcttaa aacagcaatt tgaacgttta caaaatgaac aaatctttgt ttatgtttgt
     9121 catggtaatc acgatccttt atcatcaaag atttcatcaa actggccaga taatgtttct
     9181 gtattttcaa ataaagttga gacgtatgaa gcaattacta aatctggtga aacaatttat
     9241 attcacggat ttagttatga aaatagagca agttatgaga acaagattga tgaatatcca
     9301 tcaagtcaag gccaaaaagg catacatatt ggtgtcttgc atggtacgta tagtaaatct
     9361 tcagttaacg aaagatatac cgagttcatt ttagaagatt taaacagtaa attgtatcat
     9421 tattgggctt taggtcatat acatgaacgt caacaattaa gtgatatgcc tgtaattaac
     9481 tattcaggta atattcaagg tagacatttt aatgagcaag gtgaaaaagg ttgcttatta
     9541 atcgagggtg accacttaaa attaaagact aaattttatc ctacacagta tattagattt
     9601 gaagaagcaa ctattgaaac ggataagaca tctaagcaag gtttatacga ggtcattcaa
     9661 aactttaaag aacaagtgag agaagaagga aaagcctttt atcgtttaac gcttgttatt
     9721 aatagtgaga cattaatttc acctcaagat ttattacaag ttgaagaaat gattacagat
     9781 tatgaagaaa acgaaaatca atttgtatat attgatgagt taaaaataca atatgcacaa
     9841 aatgatgagt cacctttagt taatgaattt tcagcggaat tattagtcga tcaaactgtt
     9901 tttgataaag cgatgtcaga tttatattta aatccaaggg catctaagtt cctagacgat
     9961 tatggaacat tcgaccatac agcattagtt aatcgtgctg aagaaatatt aaaagctgaa
    10021 atgagaggtg aacaaaatga taattaaatc acttgaaatt tatggttacg gtcaatttgt
    10081 tcaacgtaaa attgaattta ataaaaactt cactgaaatt tttggtgaaa atgaagcggg
    10141 taaatcgacg attcaagcat tcatccattc gatattattt ggatttccaa ctaaaaagtc
    10201 taaagagcca agactagaac cacgtctagg taaccaatac ggtggtaaat tagtacttat
    10261 tcttgatgat ggcttagaga ttgaagttga acgaattaaa ggcagtgctc aaggtgatgt
    10321 gaaagtatat ttacctaatg gtgctgtgcg tgatgatgct tggttacaaa agaaacttaa
    10381 ttatatttct aaaaagacat atcaaggtat cttttcattt gatgtactag ggcttcaaga
    10441 cattcataga aatctaaatg aaaaacaatt gcaagattat ttattacaag caggggcttt
    10501 aggatcaact gaattcacgt caatgcgcga agtgattaat cgtaaaaaag atgaattata
    10561 taaaaaatca ggtaaaaatc cgatcattaa tcaacaaatt gagcaattaa aacaactaga
    10621 aagtcaaatt cgtgaagaag aagcaaagct agaaacatat catcgcttag tagatgatcg
    10681 agataaatca tcacgtcgat tagagaattt aaagcataat ttaaatcaat tatcaaaaat
    10741 gcatgaagaa aaacaaaaag aggttgcttt acatgatcat tcacaagaat ggaagtctct
    10801 agaacaacag ttaaatattg agccaatcac attcccagaa aaaggtgtgg atcgttacga
    10861 aaaagcacga gcgcataagc aatcgttaga aagagatatt ggtttaagaa atgagcgttt
    10921 agctcaactt aaagaagaag cgactcaatt agagccagtt aaacaatctg atattgacgc
    10981 cttcattagt ttgaatcaac aagaaaatga aattaaaaat aaagaatttg aacttactgc
    11041 aatcgaaaag gatattgcga ataaacaacg tgataaagat gaattgcaat caaatattgg
    11101 ttggtctgaa acgcatcatg acgtagatag ttcagaggca atgaaaagtt atgtcagtga
    11161 gcaaatcaag aataaacaag aacaagctgc atacattaaa caattagaac gtagtttaga
    11221 agaaaataaa atcgaagata atgcggttca tagcgaacta gattctgttg aagaaaaaat
    11281 agttcctgaa gagacttttg aaaagaaaaa agaatactca caacaagtca ttgaattaaa
    11341 cgaaaaagag aacttgtata gcaaattgaa agaacgtttc gaaattgagc aacaagaaaa
    11401 acaaaaacgt caaaaattgt tacgaacaac gtttattctt ttaacattag taggtattgg
    11461 tttaactgcc ttctcattta tttcaaacaa catgttgttt ggtattattt tcgcagtatt
    11521 aactttagta tttgttatcg gtatcatcat gtctaaatca aaagaagtag attatagcga
    11581 ggccatcaca gatgaaatcg aagaaattaa agcacaacta gccattttag atgaaaatta
    11641 tgacttagat tttgatcttg atgagcaata tcgcattcgt gatcactggc aacaagcttt
    11701 gaaaaataaa gatattttag aagagaaacg tcagtatatt gaaggtcgtt taaatgatgc
    11761 gaaagggcgt catgatgaat tacaatcgac agttgagaac gttaaggatg aattatactt
    11821 atcatctaaa atttcaaatg atttaattgt tgatagtatt tctacaatgg cgaatattaa
    11881 agcattagat caacatatca gtgatttaaa tcaacaacgc cagcaattgg ttcaagaatt
    11941 agatacattc tacaatcatg ctgaagctgt tacaaaatca caatttgttt atttcaacaa
    12001 attatcttta ttccacgatg tgcaacaatg gttgaaatca gctgaagaca cgaatgaaaa
    12061 atggcgtatt aatgctgaaa ataccaaact tgttacaaat gaattaaatc atttaaatgc
    12121 tcaattagaa gaaaataata aagaaattac agcactattc gattttatca atgttggtac
    12181 tgaagaagat ttttaccaac atcatgaaga ttaccaaact tatactagta atttgagtcg
    12241 ttttaatgat ttaactaaat atcttgagaa ccaaaactat tcttatgaat taagttctag
    12301 tttaagtgaa aaaacaactg cacaacttga agaagaagat catttattgg ctactcaagt
    12361 tgacgaatat aatgagcaat atcttgaaat gcaagcacaa gtcagtgatt taagtgcaca
    12421 aatcaatcac atggaaactg atacaacgct tgctaattta agacatgaat atcacagtct
    12481 taaaaatcaa cttaatgata tcgcaaaaga ttgggcaagt ttaagttatt tacaaagttt
    12541 agttgatgaa cacattaaac aaattaaaga taaacgtttg cctcaagtta ttaatgaagc
    12601 ggtagaaata ttgaagcatt taacagatgg cagatatacg atgattaact ataatgaaga
    12661 ttcaattacg gttaaacatg ttaatggtca attatatgat cctgttgaac taagtcaatc
    12721 tacaaaagaa ttactttatg tagctttacg tatcagttta attaaggtac taagaccata
    12781 ttatccgttc ccattaattg ttgatgatgc atttgttcat tttgataaaa aacgtactga
    12841 aaaaatgttg aattatttac gatcattatc agaacactat caagtactat actttacatg
    12901 tgtaaaagat aatattgttc catcaaaaga agtgattaca ttaaacaaaa tagaggaagg
    12961 cgggaaacga tgagaaatat agagaatcta aatcccggag attcagttga tcactttttc
    13021 ttagtgcata aagctacaca gggtgtaaca gcacaaggta aagattatat gacattacat
    13081 ttgcaagata aaagtggtga aattgaagcg aaattttgga cggctacaaa aaatgatatg
    13141 gcaacaatca agcctgaaga aattgtacat gttaaaggtg acatcataaa ctatcgcgga
    13201 aataaacaga tgaaagtcaa ccaaattaga ctagcgacaa ctgaagatca attaaaaaca
    13261 gaacaatttg tagatggtgc acctttatca ccggcagaaa tacaagaaga gatttctcat
    13321 tatttgctag atattgaaaa tgctaattta caacgtatca cacgtcattt attgaaaaaa
    13381 tatcaagaac gattttacac atatccagct gctagttctc atcatcataa ctttgcgagt
    13441 ggcttaagct atcatgtatt aacgatgtta cgtattgcaa aatcaatttg tgacatttat
    13501 ccattgttaa acaaaagttt gttatatagt ggtattattt tgcatgatat tggtaaagtt
    13561 agagaattga gtggtcctgt tgcgacgtcg tatacagtcg aaggtaactt attaggacac
    13621 atctcgattg cgagtgatga agtagttgaa gcagctcgtg aattgaacat tgaaggagaa
    13681 gaaatcatgt tgttaagaca tatgatttta tctcatcatg gtaagttaga gtatggttct
    13741 ccaaaactgc catacttaaa agaagcagaa attttatgct atatcgataa tatcgatgct
    13801 agaatgaata tgtttgaaaa ggcatataaa aaaactgaca agggtcagtt tacagataaa
    13861 atatttggtc ttgaaaatcg tagattctac aatcctgaat cactcgatta agaattaaaa
    13921 gatatcggac agatgagtat actgaaacat gagctcatct gtccgatttt taatgcgaaa
    13981 aaaccgagcg gccgtggtat gatttttgaa ccacggtcgc tcggttttgt gttattggct
    14041 catgccggat tgtccgcctt gtgcgccacc ttgtttaagt ttttcagggt ttaagatttt
    14101 atcttcgaca actgatttaa tatcacgatc tttaaagtca acatcgtatt cttttaatag
    14161 atctttgtat gcatcagtca ataattttgg atttttttgt actttctgat cgactaattt
    14221 ttctttcagg ctttgttttt cactgttaaa gtctgttggt ttatcagctt taataatatg
    14281 atatccaaag cttgatttaa caacctctga tacttcacca tctttaagct taaatagtgc
    14341 tttttcaaaa tctttatcag tttgtccttt aagaacataa cctaattcgc catctttttt
    14401 agctgaacca gtatccattg attctttttt agcgatttca ccaaatttac ttggatcttt
    14461 tgaaacttct ttttgaattt cttcagcttt ttgtttcgct tctttatcat ctaagccttc
    14521 tttgtcgctt ttcttagatt taactttaat taaaatgtgt gaagctttct tgctgtcttc
    14581 tttaatttca gaatcagaga ttttaatttt atctgatagt aattctttat gataagcagc
    14641 agtacgtaaa ttttctttat atttatcggc tgttaaacct tgctgttgaa gggccttttc
    14701 aaatttatct ttaccgccgt attgcttttg cattttttca atttgttcgt caatcttctt
    14761 atcattaact ttatttttat atttatcagc taaaatttta tttaacattt cagtaaatga
    14821 tgcatttgca atttgatctt taccgatttt tttcattgta tctgcaactg ttacgtctcc
    14881 agctttagaa gaaattaatg tattttcttt agagtctgtg gcactagcgc cacaagcgcc
    14941 taataataaa gcactagctg ttaccggaac gattaatttg tttatcatct tcatagttga
    15001 aactcctttg taagttaatc atcgtgaata taacatagtt ttatattatc accaatcaat
    15061 aacccatgac atcagactag agcattaggt ttaggggtag aaattgatga aaataaaaaa
    15121 acaaggtcta tatacatttg attgtaagct attacatagc ctaaaaacaa ataataacac
    15181 cttgctataa ttattttgtt ataaatgata tggattttta atttttttca aaatattaat
    15241 acctgtacta tgttcttcta tatattgtcc ataattagaa aatataatta tttttaaata
    15301 taataaatct gtaatggaaa taccgaaatt caatgccaat aagaacataa aataatgtcc
    15361 atattgcggg aatgaaattg ttagataaac gaacattgca gataagataa ttaacggcaa
    15421 tattaagtta atgcaaaaat aaaatttgtg tacaggttga ttgacgtagg tattatagaa
    15481 tataagccat tttcttttag ttaacttatg aactttaaac gattttctat atggtaagaa
    15541 aaataataaa tgtatacttt tatgtaatgg atacgtcata aatacaagta ataaaaagat
    15601 gagaaaatgt ctatctgata atggcgtatt agataaaaaa tacattactt cataactaac
    15661 taaaaacata atgatggttg caactgcact cataaatgca attctaggca aaccaaatcg
    15721 tgcattgata tcaatttgtc ttttacataa aaacatatct tacgcctcat ctcttttcaa
    15781 tataagttag attataacta actcaggttc agtaattcaa tagtaacata tcatgttaga
    15841 agaattgtta tataagaaga aaggagcgac gtattgtttg atttgaaacg cgaaagtggg
    15901 gattggtaaa attgtctgat tgactagcgt agaagtagaa tgcagaacaa atttctgcga
    15961 acacatattc ttgctaataa agcatcactt atgaaaaagg cgcgtgtgac atgagtattt
    16021 gaaaacttga ataatataaa aaatggatat ggcagggcat atgcaaaagc atcttttgac
    16081 caactaagtt atacaaatgc taaaaatata gtg
//
LOCUS       DANRDI010000047        16097 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.047, whole genome shotgun sequence.
ACCESSION   DANRDI010000047 DANRDI010000000
VERSION     DANRDI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.047"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(208..714)
                     /locus_tag="PZ377_001196"
     CDS             complement(208..714)
                     /locus_tag="PZ377_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6358077.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            827..1750
                     /gene="graX"
                     /locus_tag="PZ377_001197"
                     /gene_synonym="apsX"
     CDS             827..1750
                     /gene="graX"
                     /locus_tag="PZ377_001197"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDM6358078.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            1766..2440
                     /gene="graR"
                     /locus_tag="PZ377_001198"
                     /gene_synonym="apsR"
     CDS             1766..2440
                     /gene="graR"
                     /locus_tag="PZ377_001198"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDM6358079.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            2433..3473
                     /gene="graS"
                     /locus_tag="PZ377_001199"
                     /gene_synonym="apsS"
     CDS             2433..3473
                     /gene="graS"
                     /locus_tag="PZ377_001199"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDM6358080.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            3617..4378
                     /gene="vraF"
                     /locus_tag="PZ377_001200"
     CDS             3617..4378
                     /gene="vraF"
                     /locus_tag="PZ377_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDM6358081.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            4368..6257
                     /gene="vraG"
                     /locus_tag="PZ377_001201"
     CDS             4368..6257
                     /gene="vraG"
                     /locus_tag="PZ377_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HDM6358082.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
     gene            6982..7599
                     /locus_tag="PZ377_001202"
     CDS             6982..7599
                     /locus_tag="PZ377_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDM6358083.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            7615..8622
                     /locus_tag="PZ377_001203"
     CDS             7615..8622
                     /locus_tag="PZ377_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDM6358084.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(9210..10007)
                     /locus_tag="PZ377_001204"
     CDS             complement(9210..10007)
                     /locus_tag="PZ377_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDM6358085.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            10368..11012
                     /locus_tag="PZ377_001205"
     CDS             10368..11012
                     /locus_tag="PZ377_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDM6358086.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            11709..13858
                     /locus_tag="PZ377_001206"
                     /pseudo
     CDS             11709..13858
                     /locus_tag="PZ377_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14004..14363
                     /gene="sarX"
                     /locus_tag="PZ377_001207"
     CDS             14004..14363
                     /gene="sarX"
                     /locus_tag="PZ377_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDM6358087.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDD
                     ISKFNALFEDVEQFLNI"
     gene            14553..15269
                     /locus_tag="PZ377_001208"
     CDS             14553..15269
                     /locus_tag="PZ377_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDM6358088.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            15269..15742
                     /locus_tag="PZ377_001209"
     CDS             15269..15742
                     /locus_tag="PZ377_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDM6358089.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            15834..15950
                     /locus_tag="PZ377_001210"
     CDS             15834..15950
                     /locus_tag="PZ377_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358090.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
BASE COUNT         5861 a         2002 c         2743 g         5491 t
ORIGIN      
        1 caacatatat aaagcaccac taatcgtagt tgatagttaa tcaattacac attagtggtg
       61 ttttattttg aatttaaatt tgataaatga ataagagtgt acgtatttat ttgagattag
      121 ataacattga taagtatgag taatgtatta ggtaaccatt aaattcgttg ttatacgatt
      181 ctgatacaag attatgataa aatagcttta attaagaatt ttagccatca tatagtcatc
      241 ataatattta ccatcgataa ataacttatc ttttaaaacg ccttcgattt gaaaatcggc
      301 acttttaaaa agctcgaggg caggttggtt attgagtggt acatttgctt caattcggtg
      361 tatttgattg tttaaacacc aagccataat ggcatcaaga agtgcttggc caattccacg
      421 atgttgatat aatttcttta cacctaaatc aattttagca acatgtttaa tgcgttgaaa
      481 tggtgtcgta ttaacaaagg caaagccaac gagttgttca tcactttcag caacgaagat
      541 gactttatgc ggagaagtga tatattcttc taattgttta ctagccgatg tgacgctagg
      601 atcatattct cctggtgtgt agaacatata cggagattcg tcgtatatgt tcgctaacat
      661 tgaaatgaaa ttttctacat ctttgatact aactctacgt ataatatggg ccataaaaag
      721 cctccagtat tttgaaattt taaaacattt gctactatta taatatatat gtaactaaaa
      781 ggtggagtaa tatgtttttt ggttacagtt taaaggagta ttttagatga aacctaaagt
      841 tttattagca ggtggaacag gatatattgg taagtattta agtgaagtga ttgaaaatga
      901 tgctgaactt tttgctatat caaaatatcc agacaataaa aaaacagatg atgttgaaat
      961 gacttggatt cagtgtgata tatttcatta cgaacaggtt gttgcagcaa tgaatcaaat
     1021 agatattgct gtattcttta tcgacccaac aaagaattct gccaaaataa cacaatcatc
     1081 agcaagagat ttaacattaa tcgcagcaga taattttggt cgagcagcgg ctatcaatca
     1141 agtaaaaaaa gtaatctaca tacctgggag tcgttatgat aatgaaacaa ttgaacgcct
     1201 aggtgcatat ggtacacctg tagaaacaac aaatttagtt tttaaacgtt ctttagttaa
     1261 tgtagaatta caagtttcaa agtatgatga tgttagatca acgatgaagg tagttttacc
     1321 aaagggatgg acattaaaga acgttgtaaa ccattttatt gcatggatgg gttacactaa
     1381 aggaactttt gtgaaaacag aaaaatcaca tgatcaattt aagatatata ttaagaataa
     1441 ggtgcgaccg ctcgcagtat ttaaaataga agaaacagct gatggaataa taactttaat
     1501 tttattgagt ggaagtttag tgaaaaaata tacagttaat caagggaagt tagaatttag
     1561 attaatcaaa gagtcggcag tcgtttatat acatctatac gattatatcc ctcgattatt
     1621 ttggccgatt tattacttta tacaagcacc aatgcaaaaa atgatgattc atggctttga
     1681 agttgactgc cggattaaag attttcaaag tcgattaaaa tcaggagaaa atatgaaata
     1741 tactaaatga tattgggtga tatggatgca aatactacta gtagaagatg acaatacttt
     1801 gtttcaagaa ttgaaaaaag aattagaaca atgggatttt aatgttgctg gtattgaaga
     1861 tttcggcaaa gtaatggata catttgaaag ttttaatcct gaaattgtta tattggatgt
     1921 tcaattacct aaatatgatg ggttttattg gtgcagaaaa atgagagaag tttccaacgt
     1981 accaatatta tttttatcat ctcgtgataa tccaatggat caagtgatga gtatggaact
     2041 tggcgcagat gattatatgc aaaaaccgtt ctataccaat gtattaattg ctaaattaca
     2101 agcgatttat cgtcgtgtct atgagtttac agctgaagaa aaacgtacat tgacttggca
     2161 agatgctgtc gttgatctat caaaagatag tatacaaaaa ggtgatcaga cgattttcct
     2221 gtccaaaaca gaaatgatta tattagaaat tcttattacc aaaaaaaatc aaatcgtttc
     2281 gagagataca attatcactg cattatggga tgatgaagca tttgttagtg ataatacgtt
     2341 aacagtaaat gtgaatcgtt tacgaaaaaa attatctgaa attagtatgg atagtgcaat
     2401 cgaaacaaaa gtaggaaaag gatatatggc tcatgaataa tttgaaatgg gtagcttatt
     2461 ttttgaaatc tcgcatgaac tggatatttt ggatattgtt tttaaacttc cttatgttag
     2521 gcattagtct aatcgattat gattttccaa tagacagttt attttatatt gtttctttga
     2581 atttaagttt aacaatgatt tttcttttat tgacatattt taaagaagta aaattatata
     2641 agcattttga caaagataaa gaaatagaag aaattaaaca taaagattta gcggaaacgc
     2701 catttcaacg tcatacagtt gattatttat atcgtcaaat ctcagcgcac aaagaaaagg
     2761 ttgttgagca acagttgcaa ttgaacatgc atgaacaaac cattacagaa tttgtgcacg
     2821 acataaaaac acctgtgaca gctatgaaat tattaattga tcaagaaaaa aatcaagaaa
     2881 gaaaacaagc attactatat gaatggtctc gtataaactc gatgctagat acacagctgt
     2941 atattactag attagaatct caacgtaaag atatgtattt tgattacgtg tcacttaaac
     3001 gcatggtcat tgatgaaata caattaacaa gacatattag tcaggttaaa ggtattggtt
     3061 ttgatgttga ctttaaagtg gatgattatg tttatacaga tataaaatgg tgtcgtatga
     3121 ttattagaca aattttgtca aacgcattga aatatagtga gaattttaat attgaaattg
     3181 ggacagaatt aaatgatcaa catgtttcgt tatatattaa agactatggc agaggtatta
     3241 gtaaaaaaga tatgccgcga atatttgaac gaggatttac gtcaacggct aacagaaatg
     3301 aaacgacgtc ttcaggtatg ggtctatatt tagtaaatag tgtaaaggat caattaggta
     3361 ttcacctgca agtcacgtcg actgttggta aggggacaac tgtcagattg attttcccat
     3421 tacaaaatga aattgttgaa cgcatgtcgg aagtgacaaa tttgtcattt taaacatgcg
     3481 ttttgttact tagaattgat acatcaatgc agcttcaacg ttataataag atagatgtta
     3541 gtcatatgtt aaattgaaga tacaagtgcc aaagcctaaa ggaaatgaag ttaagataaa
     3601 ttataggagt gttaaagtgg caattttaga agtaaaacaa ttaacaaaaa tatatggaac
     3661 taaaaaaatg gcacaagaag tgttgcgaga tatcaatatg tctattgaag aaggcgagtt
     3721 tattgctatt atgggtccct ctggatctgg gaaaacgaca ttattaaatg ttttaagttc
     3781 aattgattat atttcacaag gttctattac attaaaagga aaaaaattag aaaagctttc
     3841 aaacaaggaa ttatctgata tacgcaagca tgatattggt tttatttttc aagagtataa
     3901 tttactgcat acattgactg ttaaagaaaa cataatgtta ccactaacgg tacagaagtt
     3961 agataaagaa catatgttaa atcgttatga aaaagtagca gaagcattaa atatattgga
     4021 tattagtgat aaatatccct ctgaattgtc tggtggacaa aggcaacgaa catcagctgc
     4081 cagagcattt ataacattgc cttctattat atttgctgac gaaccaacag gtgcattgga
     4141 ttctaaaagt actcaagatt tattaaaacg attaacaaga atgaatgaag catttaagtc
     4201 tacaattatt atggtaacgc atgatcctgt tgcagcaagc tatgcaaatc gagtagtgat
     4261 gctaaaagat ggtcaaattt tcactgaatt ataccaaggg gatgacgata aacatacctt
     4321 tttcaaagaa ataatacgtg tacaaagtgt tttaggtggc gttaattatg acctttaacg
     4381 agataatatt taaaaatttc cgtcaaaatt tatcacatta tgccatctat cttttttcgt
     4441 taattacgag tgtagtattg tattttagct ttgtagcatt aaaatacgct cataaactaa
     4501 acatgacaga gtcatatcca attataaagg aaggctcaca agtcggaagc tactttctat
     4561 ttttcatcat aattgcattt ttgttatatg ccaatgtgtt atttattaaa cgacgaagtt
     4621 atgagcttgc attatatcaa acattaggtt tatctaaatt caacattatt tatatactaa
     4681 tgctcgaaca attactaata tttataatta cggcaatatt aggtattatt attggtattt
     4741 ttggttcgaa actgttatta atgattgtct ttacattatt aggaattaaa gaaaaggttc
     4801 caattatttt tagtttgagg gcggtatttg aaacattaat gttaatcggt gtcgcttatt
     4861 ttttaacatc tgctcaaaat tttatattag tgttcaaaca atctatttca cagatgtcaa
     4921 agaataacca ggttaaagaa acaaatcata ataaaattac atttgaagag gttgttttag
     4981 gcatcttagg tatagtattg attaccacag gatactatct atctttgaac attgttcaat
     5041 attatgattc tatcggtaca cttatgttta ttttattgtc aactgtgatt ggggcatact
     5101 tattttttaa aagctctgtt tctctagttt ttaaaatggt gaagaagttt agaaaaggtg
     5161 ttataagtgt aaatgatgtc atgttctcat catctattat gtatcgtatt aagaaaaatg
     5221 ctttttcact tacggtcatg gcaatcattt cagcgattac tgtttcagtt ctttgctttg
     5281 ctgctataag tagagcgtcc ttatcaagtg aaataaaata tactgcacca cacgacgtta
     5341 caattaaaga ccaacaaaaa gctaatcaat tagcaagtga attaaacaat caaaaaattc
     5401 ctcattttta taattataaa gaagtaattc atacgaaatt gtataaagat aatttatttg
     5461 atgtaaaagc gaaagaacca tacaatgtaa caattactag tgataaatac atccctaata
     5521 ctgatttgaa acgtgggcaa gctgatttat ttgtagcgga aggttctatc aaagatttag
     5581 tgaaacataa gaagcatggt aaggcaatta taggaacgaa aaaacatcat gttaatatta
     5641 agttacgtaa agatattaat aaaatctatt ttatgacaga tgttgattta ggtggaccaa
     5701 cgtttgtctt aaatgacaaa gactatcaag aaataagaaa gtatacaaag gcaaagcata
     5761 tcgtctctca atttggattc gatttgaaac ataaaaaaga tgctttagca ttagaaaaag
     5821 cgaaaaataa agttgataaa tctattgaaa caagaagtga agcgataagc tcaatatcaa
     5881 gtttaaccgg aatattatta tttgtaacat catttttagg tattacattc ttgattgctg
     5941 tatgttgcat tatatacata aagcaaatag atgaaaccga agatgagtta gagaattata
     6001 gtattttgag aaagcttgga tttacacaaa aagatatggc aaggggacta aagtttaaaa
     6061 ttatgtttaa ttttgggtta cctttagtta ttgcactatc acatgcatat tttacatcat
     6121 tagcatatat gaaattaatg ggtacaacga atcaaatacc ggttttcata gtaatgggat
     6181 tatacatttg tatgtatgct gtttttgcag tgacggctta taatcattcc aagcgaacaa
     6241 ttagacattc catataaaat atacagatgg ctttcagtag agtagtggat tcggattcac
     6301 gaactatact ggaagctttt tattataaat gaagagaagt tatattttta gcatgtatag
     6361 ttgaatactg ggttaaaata ccatattaat aatgaagtaa aggtatgagt gattatgaaa
     6421 gtgttttgaa tgaaatatat ttaattggtg atgcttttaa ttgaaaagat taacaggatt
     6481 caactttgta aattgtatta aatgtgagaa aataaaagta tattcattga gagatatatg
     6541 agtcaatgat cgttttaaac aagataagtg tattttaata tgtaaaagtt atgtaataaa
     6601 tattgtatcg ttgcaaattt cccaactata tccatttaca atttttagag ttgtatagat
     6661 aaaaaattgc ctatacaata actttttcaa aacaaatgcc tactactatt taaattttat
     6721 gtaaaagtta agtttcggaa ttgttaaaaa tttgtaaatt gaattgtggg attgtaaatt
     6781 ttgtgctatt ttcatataag ggcaaataca gtaaattgtt tactgtgagg catttttgaa
     6841 attaatatca gtacactaaa attatactga cgattgataa taacaaattt gtatcattag
     6901 tttgtaaatt cacttgtctt aatttgaaac aaattataac tgaatgtgat tggtgacaat
     6961 cgcttaaatg gaggatttta aatgtttagt aagaaaaaag ataagtttat ggttcaatta
     7021 gaagagatgg ttttcaatct ggatcgtgct gctattgaat tcggtaaaat ggatttcaat
     7081 acacatttag atttaaaagc atactcagac aacattaaaa cttatgagtc acatggtgac
     7141 gaattagtac atcaagtaat tactgattta aatcaaacat ttatcacacc aattgaacgt
     7201 gaagatattt tatcattatg tgatgcaatt gatgatgttt tagatgcaat tgaagaaacg
     7261 gcagctatgt ttgaaatgta ttcaatcgaa tacacagatg aatatatggc tgagtttgtt
     7321 gataacattc aaaaagcagt tgcagaaatg aaacttgctg tcggcttatt agtcgataaa
     7381 aaattatcac atatgcgtat tcattcaatt aatattaaag aatttgaaac aaactgtgat
     7441 ggtattttaa gacagtcaat taaacatatt ttcaatagcg aaacagatcc aatcacttta
     7501 attaaaataa aagatattta tgaaagcatg gaagaaatcg ctgataaatg tcaaatcgta
     7561 gcaaataatt ttgaaactat tattatgaaa aatagctaag gggagtatat atttatgtca
     7621 tatataatca tcgtcactat agctgtagtt attttctcgc tgatatttga ctttatcaat
     7681 ggattccatg atacagccaa tgcagtagct actgctgtat ctactagagc gttaacgcct
     7741 aaaacggcaa ttttaatggc agcagtgatg aactttatag gtgctttaac atttacgggc
     7801 gttgcaggca ccattactaa agacattgtc gatccattta aattggaaaa tggattagtt
     7861 gttgtgttag ctgcaatact tgcggctatt atttggaatt tagctacttg gttttacgga
     7921 attccaagtt cgtcttcaca tgcacttata ggttcaattg cgggtgcagc aatcgcatct
     7981 gaaggctcat ttggagtgtt acattaccaa ggtttcacaa aaattattat tgtattaatc
     8041 gtttcaccga ttatcgcatt ttgtgttggt ttcttgatgt attcaatttt taaagttatc
     8101 tttaaaaatg caaatttaac aagagcgaat cgtaacttta gatttttcca aattttcaca
     8161 gcagcgttac aatcattctc tcacggtacg aatgatgcgc aaaaatcaat gggtattatt
     8221 acgttggcat tgattgtcgc taatgtacag aatgatggca gtgttgaacc acagttatgg
     8281 gtaaaatttg cctgtgcgac agcaatgggg cttggtactg caattggtgg ctggaaaatt
     8341 atcaaaactg taggtggtaa tattatgaaa atacgtccag caaatggtgc tgcggccgat
     8401 ttatcatctg cattaacaat ttttgttgca tcatcgctac atttcccatt atcaacaact
     8461 cacgttgtgt catcatcaat cttaggtgtt ggtgcttcta accgagctaa aggtgtaaaa
     8521 tggagcactg cgcaacgaat gatcattaca tgggtgatta cattacctat ttcagcattg
     8581 ttagcaggtt tactattcta tatacttaac ttatttttct aattgaaaat aaaactaaac
     8641 tgaacttcag tatcacaaac atatggtggt attgaagttc agttttttat gtaagtagat
     8701 aacatatttt atagaattta cgtagaatga ccaagacaaa gcattttaat taggaggcta
     8761 tattataaat gtattccatg gaacataatc agttaggtca ttaaaatata tgtaagagca
     8821 aaataatgat ttaatagttt atattcttca atgaaggaat aattaaatag ttatcaactt
     8881 gtttaattaa cctaaattgt attgggcttt atttaatata aatttgtata tgtagacgaa
     8941 aaagcgtaaa atactggtat aacaaattaa atcattatat agtcaactta gttgcattat
     9001 tttgattttt aatatattat gcatttaatt taatcaaata ggtaggttgt taatttttac
     9061 ctaactacca ttcatacaaa atataaatat gtatgatagg gaagggtgag ataatttttt
     9121 gtaccaaaat gaaaattttt ttcaaaaata aaaaactagc aatctcacat gatgtgaatt
     9181 gctagtatat atcagtacaa tttatttaat taatggatga atgcatagct agaaacttct
     9241 gaagctggaa ttgtacggta gttcatattg tatggaccat atgtgtaatt catttcagaa
     9301 atcaagatac taccatcacc attgacacgt tcaacataag caacatgacc atatggacca
     9361 ggtgtgcttt gcataattga accaactgat ggtgtgttgt ttacttggta accatcatta
     9421 gctgcgttac cagcccaata cttagcgtct gaccaatatg tgctaattgg actaccagct
     9481 tgagcacgac ggtcaaatac gtaccatgta cattgaccag cagtgtataa attttggtga
     9541 ttaaaagatg atgcattgcc attgctacct gttgtagctg ttggtgttgt accacctgat
     9601 ccaccattag gaatttgtaa tgtttggtta ggcataatta aataaccacg taagttattg
     9661 gctgccatta attgatcaac tgaaacacca tatctgctag caatgatatt taatgattca
     9721 ccagcttgta cagtatgaga tgatgctgaa ccagcttgtg gagaagtgtt tgacgtattt
     9781 tgtgcatcac ttccacctac tgagataact tgaccaggga ataccaagtt gttatctaat
     9841 tggttatttt gtttaatact ctctactgaa gtgttgtatt tttgagcaat actccataat
     9901 gattcaccag attgtactgt atgttgtgta gaagcttgtg catcatgatg cgttaaaaat
     9961 gcagctgcac cagatgttgc tgttattgca aatgctaatt ttttcaaagg gactcctcct
    10021 taaaattatg ttttactact tttaaaaatt attaagacaa tttatatagt aaattaagtt
    10081 ttcgttttat attacgattt ccatattatc aaaaataagt agctttgtgt ggtttgttat
    10141 tatcttgtaa tattattaac attttaggat atcggttcat atttttataa atgctgattt
    10201 gatagtgtat aaaagcgttt tagtgatttt aactaattat aaaaaattat attttgtaac
    10261 attgtaaaat gtaaaattac tatacttgtt aaaggttatt tcttttgatg aagcaaaatt
    10321 tactgtaaaa tttgacgcat aaaacgacgg tagaaggagg aattattttg tcgcaaaata
    10381 caaatcattc atattatcat caaaaccagc atgctcaatc aataagtaaa gtgtggcttt
    10441 attttatgta ttattggatt atatttggca taggatgcta tctaggtcag tttttaccat
    10501 taagttggcg acaacccttg tcatttggat tactgattat tattttagca acacttgttt
    10561 ttgaaagagc gagacggttc ggtttaatta tttcacatat ttacgctgta gtgattggct
    10621 tattgtcata cgcaacgttt accacgtatt tacaaaattt aggaccagat attttctata
    10681 aaaatatcgc attagcaatt tttgcattta tagcatttgg tattattggt tatttcttcg
    10741 ttggagatgc atcgagtata ggcaaatatt tattcgttac attaataaca ttaattattg
    10801 cgagtctaat tggtattttt cttcaaaatc ctatttttta cactattatt accgtcgtta
    10861 gtctgttgtt atttctactt tatactttgt atgattttaa tcgtttaaaa agaggtgact
    10921 attcaccaag agaaatggga tttaatctat ttattaattt gttgaatatt attaaggata
    10981 tactttacct tgctaatatg ttcagaagat aaacagttaa attaataaag aaatttgtat
    11041 aaagttttgg aataatcaat aatgaatatc aaccttaaac tggagcgatt gtcataagtt
    11101 agaaacttag actgagattt tactgataaa gcaataaaaa tatattacct taattgtaaa
    11161 gatattatta atcattgaaa tgattaactg ctatagttct aaagtaaaca aatcttagaa
    11221 gataaacttt aaacaggtgt acttgccttt ctaattacac ttttattaga aaagtaaagt
    11281 atagctacgt attaatttta aaatcgaaac attaatagga agaagtatag ataatataaa
    11341 caatttaatg ttagtttgag ctcatctcaa aagtagaatg tgatttaaat aagtgtttat
    11401 ttttaaattg aaaatgaata tataactaac aaaagataat agtaatacgt tgtagatgtg
    11461 gtcatttaaa attattttta aaatactaaa aagtagtgtg ttttcaattg gatagtttaa
    11521 ttaaactgac tacatctatt tttgtaatat ttaaatgatt gatatataag tttaaaaatt
    11581 aaccattata tagttttatt tatttttgat ttggtattcg tattgtgtta aatttacgtt
    11641 taattaaata agttaaatat tatagggaat gataaaagtg gtacttaaat atagaaagag
    11701 gtaaagttat ggcaaaatca tgcttgcata tacttactaa taatgaatat gcgacaacgc
    11761 gttgccaaga tggcatagtc ttattttggc caattgacgg ggaaatcgaa ctacaaaaat
    11821 ttcgtaaaag taaaataatt gaagatgata tatatattat taatcatctg gatgtattta
    11881 gtattaagaa taataaaaaa acgatcatgt tgtatttgag tagcgattgg tttgcggaat
    11941 taggctttac tttctttaat taccactata cagcaaagtt gattaaatca tcctataatt
    12001 tgaaatgtct actattaaaa ttgacatatc gataccttga taatcagcct cttaatgacg
    12061 ctgatattag aaaattacag gatattatta aaatcattgc aaaagaagca agtatggata
    12121 aaaagattgc acaaaatcaa tatcgatatg cgtattatgg tgatttgcgt gatgagctcg
    12181 aatatattta tcaaaatgta aatcaacgat tgacattaaa aagtgtcgct gataaattat
    12241 ttgtctcaaa gtcaaatttg tcatcacaat tccacttact tatgggcatg ggttttaaaa
    12301 aatatattga tactttgaaa attggtaaat cgattgaaat tctacttact actgatagta
    12361 ctattagcaa cataagtgaa catttaggtt ttagtagtag ctccacttac tctaaaatgt
    12421 ttaaaagtta tatggatata acaccgaatg aatatcgtaa tttatcaaaa tataataaat
    12481 gtttaatgct aaagccagaa ccactagtag gcaaaatggt gcaagaagta aaagaaatca
    12541 tattgaatta tattgaacat tataaaaacc acctaactga tgttatacat attgatgaag
    12601 acaaatttga aacacctaaa ttgtttcaaa cggttattca aataaatact tatacagaaa
    12661 tgaaattagt tttcttagaa ggaatcttta aaaccttatt gaataagaac agtcaagttg
    12721 tctttttcat catgccatcg attctaaaaa gtaaaaatac catgtccgaa gaagaaaaat
    12781 tcacaatcat taaaacaata attgaaagtg atctaaagat agcatttaat ataaataata
    12841 ttgaaacaac ttattttgtt gaagaagctt ttatgagtgt tttcagacaa atatctccaa
    12901 acgaattaag taatcataat aattacgaag tgcattttgt ttttgattta tcattgatgg
    12961 aaattagaac aatttatcga atgatattaa aattacataa catcatgttg aatgtgaaat
    13021 taggattgaa cattacctgt ttatttgaaa aaccttcagt ttttaaatca ctagtatcac
    13081 aaataaagcg acttaaattc gattcgttaa taatagataa tgcaaattta agtagccctt
    13141 atttgatggg ggaaagtgat gagttactat tgaaaaatat tttgcatttt aaaaatttaa
    13201 aacaagtaat taatgaattg gatattgaac aagaaaagct gatttttcta aatgttgaaa
    13261 atcataaact gcttaataat aaagaacgag atctaagtaa tagtgctcca ttaatttata
    13321 agacattaag tgcgctgtat cacaactttg atggctttgg attaaacatt tttgacaatc
    13381 atcaagcatt taatgcgatg catctatatg acaaaaatgg atttaaaaca acactaggtc
    13441 ttatattaga aaagtttatc gaatatgtct cgaaaccaaa atacgaaaac agttattatt
    13501 ctatttttga tatagagaat tattattgtc ttgttattta tgattggcga gtgatagaga
    13561 gcgagacaat tatgagtaat tttgaggata gtcaagttta tataaatttt aaaaacaatg
    13621 ttttaaacga taaatatcta attgtaatag aaacattgga caaaatagtg gcaacattaa
    13681 tcatttgatt tctaaagaat taagagataa atatgaatgg aaccctagtt tactatctaa
    13741 aattgacaac taccttaaac cagcaataga gattaaagag cataatttta gtgataattc
    13801 cttgaatatt aacgttactt ttaatgcgtt atacataatt aaaataggaa aaaaataaca
    13861 ccttgatatg tattgcaaaa tttaatttgc atttgttggt aattttgaca acgtattaaa
    13921 aacaaatgag aaagacaatg gctgaaaaat ttaagataat aatgacagaa gcattgtctt
    13981 tatatatttg ggggtgcaac attttgaata ctgagaaatt agaaacattg cttggcttct
    14041 ataaacaata taaagcatta tctgaatata ttgataaaaa atataagttg tcgctaaatg
    14101 atttagcagt cttagattta acgatgaagc attgcaaaga tgaaaaagta cttatgcaat
    14161 catttttaaa aactgcaatg gatgagctag atttaagtag gacaaaatta ttagtttcta
    14221 taagaagact aattgaaaaa gaaagactta gtaaagttag atcatctaaa gatgagcgta
    14281 aaatttatat ttatttaaat aatgatgata tatctaaatt taatgcttta tttgaagatg
    14341 tagaacaatt tttaaatatt taattgaaat tgagtgtcga aagcatagaa tttgcttatc
    14401 ggcacatttt taatttatac atattttaaa actaagtaac agtttgaaga aatcgtagtt
    14461 caataatgtt aattgtgaaa atgtatataa acataaaaaa atcatgtata atatatgttg
    14521 ttaattaaac agttcgaaag cgagatgaca ttatgggacg taaatggaat aacattaaag
    14581 aaaaaaaggc ccaaaaagat aaaaacacaa gtagaatata tgcgaaattt ggtaaggaga
    14641 tttatgttgc agcaaaatct ggtgaaccca atccagaatc taaccaagct ttaaggttgg
    14701 tgcttgaacg cgctaagaca tattcagtgc cgaatcatat tattgaaaaa gcaatagata
    14761 aagctaaggg tgctggagac gaaaactttg atcacctaag atatgaagga tttggcccaa
    14821 gcggatcaat gctaattgtt gatgcgttaa caaataatgt aaatcgtact gcctctgatg
    14881 tgcgagctgc ttttggtaaa aacggcggta atatgggtgt atctggatca gttgcttata
    14941 tgtttgatca tgtggcaaca tttggtattg aaggaaagtc tgttgacgaa atacttgaaa
    15001 cattaatgga acaagatgta gatgtaaatg atgtgattga cgataatgga ttgacaatag
    15061 tctatgctga accagatcaa tttgcagtcg ttcaagatgc gcttcgtgca gcaggtgttg
    15121 aagaatttaa agttgctgaa tttgaaatgt tacctcaaac agatattgaa ctttctgaag
    15181 cggaccaagt aacatttgaa aaattaatcg atgcattaga agatttagaa gatgtacaaa
    15241 acgtattcca taatgtggat ttgaaataat gaaatcagca gaacaatgga ttgatgaatt
    15301 gcaacttgaa tcacatcctg aaggtggttt ctatagagag acaattcgag aagtattgaa
    15361 agatggacgc agagcgccgt ttagtagtat ttatttttta cttacagatg acaatatttc
    15421 gcattttcat cgaattgatg ctgatgaagt atggtactat catgctggcg attctctaac
    15481 aattcatatg ataaatccgg atggggaata tacgactgca acattgggta ctgatatcca
    15541 aaatggagat gtattgcaat atgtagtgcc taaaggaaca atttttgctt cttcaatcga
    15601 aatatcaaat acttttagtt tagtaggttg tatgtgtcaa ccggcatttg agtttaagca
    15661 gtttgaattg tttaagcaat ctgaattaat tacgcaatat ccgcatctta aatcagtaat
    15721 cgaaaaatat gctttaaaat aaaagtgatc aatgaagtgg tttgaaggtt gttaataaac
    15781 ctttgagtca cttcattttt atatgtattc ttgattgaat cagaatagat ttgatgcttc
    15841 agctgttttt aatgaaaata gcattaaatg attttgaaaa cgataagagt gtgttattta
    15901 tatttttgaa aaatcacttt tatgaagaaa tgtgtgtact atctaattaa tattgtctat
    15961 tttaagtaaa ttgaagcgag ttgtagtaaa ataataataa atattttcat atgattaaca
    16021 aaaactataa aactgtatca tgacatgaca tcatttatgt agttttatat aataatgaca
    16081 aggagttggt gactgtg
//
LOCUS       DANRDI010000048        15732 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.048, whole genome shotgun sequence.
ACCESSION   DANRDI010000048 DANRDI010000000
VERSION     DANRDI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15732
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.048"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(120..1022)
                     /gene="cvfB"
                     /locus_tag="PZ377_001211"
     CDS             complement(120..1022)
                     /gene="cvfB"
                     /locus_tag="PZ377_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDM6358091.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1169..2770
                     /locus_tag="PZ377_001212"
     CDS             1169..2770
                     /locus_tag="PZ377_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDM6358092.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3630..4835
                     /locus_tag="PZ377_001213"
     CDS             3630..4835
                     /locus_tag="PZ377_001213"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDM6358093.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4899..5888
                     /locus_tag="PZ377_001214"
     CDS             4899..5888
                     /locus_tag="PZ377_001214"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDM6358094.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5890..6777
                     /locus_tag="PZ377_001215"
     CDS             5890..6777
                     /locus_tag="PZ377_001215"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDM6358095.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6774..7496
                     /locus_tag="PZ377_001216"
     CDS             6774..7496
                     /locus_tag="PZ377_001216"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDM6358096.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7523..8242
                     /gene="dapD"
                     /locus_tag="PZ377_001217"
     CDS             7523..8242
                     /gene="dapD"
                     /locus_tag="PZ377_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDM6358097.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8385..9536
                     /locus_tag="PZ377_001218"
     CDS             8385..9536
                     /locus_tag="PZ377_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDM6358098.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9541..10626
                     /locus_tag="PZ377_001219"
     CDS             9541..10626
                     /locus_tag="PZ377_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDM6358099.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10616..11881
                     /gene="lysA"
                     /locus_tag="PZ377_001220"
     CDS             10616..11881
                     /gene="lysA"
                     /locus_tag="PZ377_001220"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDM6358100.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12121..12522)
                     /gene="msa"
                     /locus_tag="PZ377_001221"
     CDS             complement(12121..12522)
                     /gene="msa"
                     /locus_tag="PZ377_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HDM6358101.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
     gene            complement(12719..12919)
                     /gene="cspA"
                     /locus_tag="PZ377_001222"
     CDS             complement(12719..12919)
                     /gene="cspA"
                     /locus_tag="PZ377_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDM6358102.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            complement(13090..13398)
                     /locus_tag="PZ377_001223"
     CDS             complement(13090..13398)
                     /locus_tag="PZ377_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDM6358103.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            13561..13830
                     /locus_tag="PZ377_001224"
     CDS             13561..13830
                     /locus_tag="PZ377_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDM6358104.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            13849..14478
                     /locus_tag="PZ377_001225"
     CDS             13849..14478
                     /locus_tag="PZ377_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDM6358105.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            14510..15646
                     /locus_tag="PZ377_001226"
     CDS             14510..15646
                     /locus_tag="PZ377_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDM6358106.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
BASE COUNT         5609 a         2474 c         2749 g         4900 t
ORIGIN      
        1 cctattcgtt tgaattattt aattttttac tttgaaagga atgtttaaaa aactacttca
       61 tctgtccgat ttaatattga ataaaaaaaa cacgcatcct acgacacgtg taaaaatgat
      121 tattcttttg agtccattcg actccaacct tttttagtta aagcgatttt acctgtttct
      181 atattaataa tcttctgttt atataagtga ccgattgcac gtttgaatga acctttactc
      241 atattgaata cttctttaat cgcttcaggg cttgatttgt cccagaatgg taattcacca
      301 tcatattcaa ctagtaaatc aaagatgact tggccgtcat cgtctaaacg ttcatgtgca
      361 agtggtaaaa atgaaccatt taactcacct ttatcattat gcccgataat tctaacttga
      421 acagattcac ctaatcttgg ttcagcttta cgttctgatt catgtacgaa aattttgtaa
      481 cctgattcgc ttaataaaaa gctaccaatt cgtaatacgc ggtaaggttt ggcttcaatg
      541 acttcgtttt ttaaattatc gtcgtgtaca ggtgtaaaca tattttctac aacagattca
      601 ctcgctaaac gtccatacat atgattctca cggtcaattc gtaatgtaac tagcaaataa
      661 tcaccaggtt gtggccatag tgatttcact tttggtaaat cttcccatgg tactaacact
      721 tcacggggta atccaacatc tatacgtgcc ccatcgcgat ccgttttaag tactttagca
      781 aagtcatatt tatctttcgt aatatcaggc atattttgag ttgcaaataa ttcacctgaa
      841 cggtttggat aaatgaagaa actatattct tcacctactt ctaattcatc atcatcgttc
      901 atttctgatt ggtttaactt tacgttttca ccgtttggtc cttttaaaag gtaagttgaa
      961 ccttgtaacc ctactacttc aaggaattct atagaaccta ctatatcttt gtctaatgcc
     1021 atgttattct ccttcgattc ttgtattctt tattattata acatgtgtga gtcattctat
     1081 cattacaact tatatgaggt cgaaactgtg acgataatat gatataattc ttccgttaga
     1141 atatcgaatg aaaaaaagga gaaaatgcat gttacaagta actgatgtga gtttacgttt
     1201 tggagatcgt aaactatttg aagatgtaaa tattaaattt acagaaggta attgttatgg
     1261 attaattggt gcgaatggtg caggtaaatc aacattctta aaaatattat ctggtgaatt
     1321 agattctcaa acaggacatg tttcattagg gaaaaatgaa cgtctagctg ttttaaaaca
     1381 ggaccactat gcttatgaag atgaacgcgt gcttgatgtt gtaattaaag gtcacgaacg
     1441 tctttatgag gttatgaaag aaaaagatga aatctatatg aagccagatt tcagtgatga
     1501 agatggtatc cgtgctgctg aacttgaagg tgaatttgca gaaatgaatg gttggaatgc
     1561 tgaagctgat gctgctaacc ttttatctgg tttaggtatc gatccaactt tacacgataa
     1621 aaaaatggct gaattagaaa acaaccaaaa aattaaagta ttattagcgc aaagtttatt
     1681 cggtgaacca gacgtactat tactggatga gcctactaac ggtctcgata ttccagcaat
     1741 cagttggtta gaagatttct taattaactt tgataatact gttatcgtag tctcgcatga
     1801 ccgtcatttc ttaaataatg tatgtactca tatcgctgat ttagacttcg gtaaaattaa
     1861 agtttatgtt ggtaactatg atttttggta tcaatctagt cagttagctc aaaagatggc
     1921 tcaagaacaa aacaagaaaa aagaagaaaa aatgaaagag ttacaggact ttattgctcg
     1981 tttctcagct aacgcttcta aatctaaaca agcaacaagt cgtaaaaaac aactcgagaa
     2041 aattgaatta gatgatattc aaccatcatc aagaagatat cctttcgtta aattcacacc
     2101 tgagcgcgaa atcggtaatg acttactaat cgttcaaaat ctatctaaaa cgattgacgg
     2161 tgaaaaagta ttagataata tttcattcac aatgaatcca aatgataaag caattttaat
     2221 tggggatagt gaaattgcga aaaccacatt gcttaaaata ttagccggtg aaatggaacc
     2281 agacgaaggt tcatataaat ggggtgtaac aacgtcatta agttacttcc ctaaagataa
     2341 ctcagaattc tttgagggcg ttaatatgaa ccttgtagat tggttaagac aatacgcacc
     2401 agaagatgag caaactgaaa catttttacg cggcttctta ggccgtatgc tatttagtgg
     2461 agaagaagtt aagaaaaaag ctagtgtact ttcaggtgga gaaaaagtac gttgtatgct
     2521 aagtaaaatg atgttatcaa gtgcaaacgt tcttttactt gatgaaccaa caaaccactt
     2581 agacttagaa agtattactg ctgttaatga tggacttaaa tcattcaaag gttctattat
     2641 ctttacttca tatgactttg aatttatcaa tacaatcgca aaccgtgtta tcgatttaaa
     2701 taaacaaggt ggcgtttcaa aagaaattcc atatgaagaa tatttacaag aaatcggcgt
     2761 tttaaaataa acataatatg tagaatttct tttcgaaaat ctctgtattg aggcccgacc
     2821 aacttgcaca ttattgtaag ttggcttact gtcaccttct atattgggac ttattccgca
     2881 acttgtattg tttgtagaaa ttaggaatcc aatttctcta tgtgttgcac cgacaaggat
     2941 tgaaaaaagc tagctacaaa gtcattttca ttcagtcaac tactgtcaat ataacattgc
     3001 agggcctagg gcattgattc atgtccgaga ctatattcaa tttaatagac attaactggg
     3061 cgtctcgatg ctaatattta tagcatcgag gtgtcctttt tattttattt tttaacacat
     3121 cagcatagtg aacacccctt ctctaaatta caaatacagt tatcaacgca ttaaaagagt
     3181 acatttattt aatgtgcata ttgattatgc tataagtatt ttgaatttta tttttcaaaa
     3241 agtctcatta tcaccacctt ttaaaatata agcaacgcta taacttgaat tttcaggtta
     3301 ttccaaaaaa tctaaattat cggtttacat tcatatttgt catatgtata atgaacacat
     3361 accaatttaa taatattttg atgaggcgca tcaatcatga gtaaagttta gattactgtc
     3421 tgctaacagc taaatttgaa agggtgcgat gccgaagcaa ttataatagc agttataatt
     3481 tgttggactt tttggttaag agctgagagt ttgtcattat ttaaaaataa tggagtgcat
     3541 cacttgtata tagattaaga gcaagttcgc attccgaact tgttcttttt ttattattgt
     3601 gtgcccttcc taacaattag gaggattata tggtaacaag aagtgtgttg aaatttggcg
     3661 gatcatccgt cagtgatttt acaaaaataa aaaggatcgc tgaaatgtta aaggagcgag
     3721 tcaatcaaga tgaacaatta attgtcgttg taagtgctat gggtaacaca acagatcaat
     3781 taatgacgaa tgtatcaacc ttgactaaag caccaaaaca acaagaactg gcattattat
     3841 tgacaaccgg agagcaacaa actgtatctt atttatcaat ggtattaaat gatatcggta
     3901 tgaatgccaa agcaatgact ggctatcaag cgggtattaa aaccattggc catcatttaa
     3961 aaagtaaaat tgctcaaatt aatcctcaaa catttgaaca agcctttcaa gaaaacgata
     4021 ttttagtagt tgctggattt caaggcatca atgaacatca ggaattaaca actttaggca
     4081 gaggtggttc tgatacgacc gctgtggcac ttgctgttag taatcaaata ccttgtgaaa
     4141 tttataccga cgttgatggt gtgtatgcca ctgacccaag acttttacca aaggctaaac
     4201 gactagacat cgtctcatat gaagaaatga tggaaatgag cgctttaggt gctggtgtac
     4261 ttgaaacaag aagtgttgaa ttagctaaaa actataatat ccctttatat ttaggaaaaa
     4321 ctttatcgaa cgtgaaagga acatggatta tatcaaatga agaaatatta gagaaaaaag
     4381 cagttactgg tgtggctttg gataaacata tgatgcatgt aacaattagt tatcccctac
     4441 ctgacaatca gctacttacc caactattta cggaacttga agaaggtgct gtaaatgttg
     4501 atatgatttc acaaatcgtc aacttggatg ggctacaact atccttcacg attaaagata
     4561 gtgattttca tcaaatttct atgattcttg aaacattaaa gaatcaatat gaagcattag
     4621 cttataaaat caatgagcat tatgtcaaaa tttcattaat tggctcaggc atgcgtgata
     4681 tgtcaggtgt ggcatcaaaa gcatttttga cattaattga aaataatata cctttctacc
     4741 aaacaacaac atctgaaata agtatttcat acgtcattga tgattttaat gggcaacaag
     4801 cggtagaaaa actatatgac gcatttaaca tttaatggta aaatgattgt taaaatattc
     4861 taaaaattgg aaattattat aaaatggagt gacaagttat gacaaagtta gcagttgtgg
     4921 gtgcaacagg attagtaggt acaaaaatgt tggagacatt aaatcgtaaa aatattcctt
     4981 tcgatgaatt agtattattt tcatcagcac gttctgcagg gcaagaagtt gaatttcaag
     5041 gaaaaacata tacagttcaa gaattaactg atgctcgtgc aagtgaacat ttcgattatg
     5101 tattaatgag tgctggtggc ggtacaagcg aacactttgc cccacttttt gaaaaagctg
     5161 gtgcaatcgt tatagacaat tcaagtcaat ggcgtatggc agaagatatt gatttaatcg
     5221 ttccggaagt caatgaacct acatttacaa gaggtatcat tgccaatcca aactgctcta
     5281 cgattcaatc tgttgtacct ctaaaagtat tgcaagatgc ttatggttta aaacgagtgg
     5341 catatacaac atatcaagct gtatcaggtt cagggatgaa aggtaagaaa gatttagctg
     5401 aaggtgtaaa tggtaaagca ccagaagcat atccacatcc aatttataat aatgtgttac
     5461 cgcatattga tgtgttttta gaaaacggat atacaaaaga agaacaaaaa atgattgatg
     5521 agacgagaaa aattttaaat gcgccagact taaaagtaac agcaacatgc gcacgtgtgc
     5581 ctgttcaaga tagtcatagt gttgaaattg atgtaacgct tgacaaagaa acaacagcag
     5641 aagatattaa agcgttattt gatcaagatg accgcgttgt tttagtagac aatccagaga
     5701 acaatgaata tccaatggca atcaattcta ctaataaaga tgaagtgttt gttggccgta
     5761 tacgtagaga tgattcatta gaaaatactt tccatgtatg gtgtacatca gacaatttat
     5821 taaaaggtgc tgcattaaat gctgtacaag tattggaaca agttatgcgt ttaaaaggag
     5881 cgaattaaaa tgacacattt atttgagggt gttggcgttg cacttacaac cccttttaca
     5941 aataacaaag ttaatattga agctttgaaa acacacgtta attttttact agaaaataat
     6001 gcccaagcaa tcatcgttaa tggaactact gctgagagcc ctactttaac aacagatgaa
     6061 aaagaacgca ttctaaaaac agttattgat cttgtagata aacgtgttcc tgtcatagca
     6121 ggaactggca ctaatgatac tgaaaagtca atccaagctt caatccaagc taaagcctta
     6181 ggggctgatg caattatgtt aattacgccc tactacaaca aaacgaacca acgtggttta
     6241 gtcaaacact ttgaagcgat tgcagatgct gtgaaattac cagtcgtgct gtacaatgtt
     6301 ccttcaagaa cgaacatgac aattgaacca gaaactgtag aaatattaag tcaacatcct
     6361 tatatagttg ctttaaaaga tgctacgaat gattttgagt atttagaaga agtgaaaaag
     6421 cgcattgata caaattcatt tgcattatat agtggcaatg atgacaacgt cgtcgaatac
     6481 tatcaacgtg gcggtcaagg ggttatctct gttattgcca atgtcattcc taaagaattt
     6541 caagcgttat acgatgctca acaaagtgga ttagatattc aagatcaatt taaaccaatc
     6601 ggcacactgt tatcagcttt atcagttgat attaacccaa ttcctattaa agctctaaca
     6661 agttatttag gatttggaaa ttatgaatta cgtctaccat tggttagcct agaagataca
     6721 gatactaaag tgcttcgtga aacatatgac acatttaaag cgggtgaaaa tgagtgaaaa
     6781 tattactaat tggctatggc gcaatgaatc aacgcgttgc tagattagca gaagaaaaag
     6841 gacatgaaat cgttggggtc attgaaaata caccgaaagc aacaacgcca tatcaacaat
     6901 atcaacatat tgcagatgtt aaaggtgccg atgttgcaat agatttttca aatccaaatc
     6961 tgcttttccc tttattagat gaagattttc atttgccatt agttgtggca acaactggcg
     7021 agaaagaaaa actacttaat aagttagatg aattgagtca aaatatgcct gtgtttttca
     7081 gcgcgaacat gagttatggc gttcatgcat tgactaaaat tttagcagct gctgttcccc
     7141 tacttgatga tttcgacatc gaattgactg aggcacatca taataaaaaa gtagatgcac
     7201 caagtggtac gttagaaaaa ttgtatgatg tgatcgtatc tttgaaagaa aatgtaacac
     7261 ctgtgtatga tagacatgaa ttaaatgaaa aacgccagcc acaagatatt ggtatacatt
     7321 ctattcgtgg aggtacgatt gtcggtgaac atgaagttct atttgctggc actgatgaaa
     7381 cgattcaaat cacgcatcgt gcacaatcaa aagatatttt tgcgaatggt gcaatacaag
     7441 cagcagaacg cttagttaat aaaccaaacg gcttttatac gtttgacaac ttataaacat
     7501 attaaaggag atcgattatt ttatggtaca acatttaaca gctgaagaaa ttattcaata
     7561 tataagtgat gctaaaaagt ctacaccaat aaaagtatat ttaaatggta attttgaagg
     7621 catcacatat ccagaaagtt ttaaagtatt tggttcagaa caatctaaag taatcttttg
     7681 tgaagcggat gattggaaac ctttttacga agcatatggt agtcaattcg aagatataga
     7741 aattgaaatg gatcgtcgca attctgctat tccattaaaa gacttaacaa atacgaatgc
     7801 acgaattgaa ccaggtgcgt ttattagaga acaagccatt attgaagatg gtgctgtcgt
     7861 tatgatgggc gcaacaatta atattggcgc agtcgttggc gaaggtacaa tgattgatat
     7921 gaatgctact ctcggtggtc gtgctacaac tggtaaaaat gtacatgtag gggctggcgc
     7981 agtattagca ggtgtgattg aaccccctag tgcttcaccg gttataatcg aggatgatgt
     8041 attaatcggt gcaaatgcag ttattttaga aggtgtacgt gttggtaaag gtgctattgt
     8101 tgcagctggc gcgattgtga cacaagatgt accagctggt gcagttgttg ctggtacacc
     8161 tgcaaaagtg attaagcaag cttctgaagt acaagatact aaaaaagaga ttgtagcagc
     8221 attaagaaaa ctgaatgact agtgacgtca acgtataata atttcaaggt tgagatacga
     8281 ttatgtctca accttattgt ttagcattga ataaacttat gatcacaaat ttcgaaatac
     8341 gttacactat ttatatagta atttaacaca aaggattgat aattatgaat gaattagaat
     8401 ttgttacgaa acatcgccgt catttacatc aacatcctga attaagctta catgaatttg
     8461 aaacaactgc ttatattaaa gcgtttttag atagtttaaa tattaaatac gattgcccat
     8521 tggaaactgg cgtcattgca tacttagaag gtaatggctc acatacgata gcgtatagag
     8581 ctgatattga tgcgttacct attttagagg aaaatgatgt gccttatcgc agtcaatctg
     8641 atcatgtgat gcatgcttgt ggacatgatg gtcatacaac tgcattaatg ctttttgtac
     8701 aacgttgcaa agacatgcaa gatgcaggtc aattaccgca aaatgtcgtt ttcattttcc
     8761 aacctgcaga agaaactggt ggcggtgcaa atcgattaat aaaagccggt gcctttgata
     8821 agtatccaat tgaagcggta tttggtattc atgttaaccc atttgctgat gaaggcattg
     8881 cagtgataag agatgaagaa attacggcca gcgcaacaga gtatcgcttt ttcttaacag
     8941 gcctgtcaag tcatgttgct gataaagaac aaggtcattc ttgtggtgaa gcattacaac
     9001 atgtattaac tcaaatatca caaattcaac aatttcacct taacggtttg aaacgaaata
     9061 ttgttcatat tggtcatttt aaagctggtg aagcgattaa cactgtacca agtaatggct
     9121 atttagaagg tactattcgt acatatgata ttgatgattt aacaatcgtt aaaaatcaaa
     9181 tgcacaagat agcagaaagt gtcaagcttc tgtttaatgt agattgtgaa gttaaatttg
     9241 cagaaggtta tccccctaca atcaatagtc cgaaattacg tactcaaata gaggacgcct
     9301 taataaaagc tgatttaaat gtctatgaca aaccaacgcc attcttattt ggggaagatt
     9361 ttagttttta tggtcaacaa ctagctccag cttactttgt ttttatagga acacgaaatg
     9421 aagataaagg ttttgtaact ggtttgcaca catcacattt aaattttgat gaaaaagtgt
     9481 taataaacgt ggttaatttt tacgaaaatt tattaaataa ttacaaagag gtgtaataca
     9541 ttgacagcaa catggtctgt aaataagaaa atatttttac aaaatgcaat cacagtcaaa
     9601 aacaatcagc cattaatggc agttgttaaa aataatgcat atcactatga cctagaattt
     9661 gctgtaactc agtttatcca tgcaggtata gatacattta gcacaacatc actacgagaa
     9721 gcaattcaaa ttagacaact tgctccagat gcaacaatct ttttaatgaa tgcagtttac
     9781 gagtttgatt tagtccgtga acatcaaata catatgactt tgccgtcgtt gacatattac
     9841 tataaccata aaaatgattt agctggtatt catgttcact tagaatttga aaatttatta
     9901 catcggtctg gatttaaaga tttaaacgaa attaaagaag tattgaaaga tcaccatcat
     9961 aatcaaaatg caaaaatgat tattagtggt ttatggaccc attttggata tgctgatgaa
    10021 ttcgatgtgt cagattataa tgttgaacgt tcacaatgga tggaaattgt tgaagcactt
    10081 ttatctgaag gttatcagtt cgacctaatc catgctcaaa atagtgcgag tttttatcgg
    10141 gaaggacaaa tattactacc ccaccataca catgcgcgtg taggtattgc gttatacggt
    10201 tcaagaccat atagttcact gaatcaacat gatatagttc agtcattaac tttaaaagca
    10261 catgttattc aagtgcgcga agtacaagct ggtgattatt gcggttatag ctttgccttt
    10321 gaagtgacta aaaacaatac aaaattagct gtagttgata tcggttatgg cgatggaatt
    10381 ttaagaactc gtgctaaaca tgaagcactt atcaatggta aacgctaccc gatacgtgca
    10441 ttaatgatga gccatatgtt tgttgaagta gatggcaatg tacatgcaca agatgaagtt
    10501 attctttata ataatgatat ccgcatcgat gaatatacct ttaaaggtgt tggtgcaaat
    10561 tctgaacaat taagtgctat gaatcatgat tctttaaaaa aggagtacat ttcaaatgac
    10621 tgttaaatat aatcaaaatg gcgaattaac aatggatggt attagtttaa aaacgattgc
    10681 acaaagcttt ggtacaccta ccattgttta tgatgaacta caaattagag aacagatgcg
    10741 ccgttaccat cgcgcattta aagatagtgg attaaaatac aatatttcat acgcctcaaa
    10801 ggcatttact tgcattcaaa tggtcaaact tgtagctgag gaagatttac agttagatgt
    10861 tgtttctgaa ggtgaattat atacagcttt agaagcaggt tttgaaccga gtcgcatcca
    10921 tttccatggt aacaataaaa cgaaacatga aattaggtat gctttagaaa ataatatcgg
    10981 ttattttgtt atagattcat tagaagaaat tgaattaata gaccgctatg ctaatgatac
    11041 ggttcaagtt gtattacgag ttaatccagg tgttgaagca catacacacg aatttattca
    11101 aactgggcaa gaagatagta agtttggatt atcaattcaa tatggcttag ctaaaaaagc
    11161 aattgacaaa gtccaacaat ctaaacactt aaaattaaaa ggtgtacatt gtcatattgg
    11221 ttcacagatt gaaggtacag aagcatttat tgaaactgct aaaattgttt tacgttggct
    11281 taaagagcaa ggcattcaag ttgaattatt aaaccttggt ggtggctttg gtattaaata
    11341 tgttgaaggt gacgaaagtt tccctatcga aagtggtatt aaagatatta cagacgcaat
    11401 aaaatccgaa attaaagttc taggtataga tgcaccagaa ataggtattg aaccgggacg
    11461 atcaattgta ggtgaagctg gcgttacttt atatgaagtt ggaaccatta aagaaattcc
    11521 agagattaat aaatatgttt caatcgatgg cggtatgagt gatcatatca gaactgcact
    11581 ttatgacgca aagtatcaag cattgcttgt taatagaaat gaagaagcag atgacagtgt
    11641 aactatagct ggaaaattat gtgagtctgg tgatatcatt attaaagacg ctaaattacc
    11701 ttcatcagtc aaacgtggag actatcttgc tatattatca actggtgcat atcattactc
    11761 tatggcatcc aattacaatc aaatgcaaaa gccttctgtg tttttcttaa aagatggcaa
    11821 agcacgtgaa gttataaagc gacaatcgtt aagacaactc attattaatg atacaaaata
    11881 aaaataaaca aagtaatccc cgaagcacag aaaattatac tgtgactcgg gggattttta
    11941 tgcattatag atgactgtag aaaacatgct tggacatata ctctaaaaaa ggaagaacag
    12001 cacattttta gttagaaagt atttatttct ttttgtcatt aatcctttat atttagttag
    12061 ccctattcaa ctaatttaaa gcatcatata cataccacac tttaaacaag catcatgaaa
    12121 ttagatttta tgctttactt gctttttcaa ataatataaa acgataagaa ttaacaacaa
    12181 atataatgta taaaacagaa tttcatcaaa tccaaaatct ttacttgtaa ttaatacatc
    12241 aatatcttct aattccatca tctctgcttt aatataatta ttaaactttg gcaaagtcat
    12301 taaatgtaca ttgtaaatat agtaataaaa gtcgataaat atgatgttaa aaaatatgta
    12361 tgttttatca atattagttt tgaaaaatag tatcccagaa atcattatcg gaatcactaa
    12421 tacaatcatc attgctgcta aaatattaat atttaatcca attgcagata atacaccgaa
    12481 aactaataaa ttagcaacta aactaaggat aagatatttc ataaaatcac ctcgtcgttg
    12541 tacaccataa ttatattata taaatatatt tttctattgc aaacaatgtt ggtgatataa
    12601 aaaaagagtt ctcaatttgg ttgagaaact catcgcgtta agtatttgta ataagcatat
    12661 aaaaacacct tacttcttgg taaggtgtta gttaaaatca atgattcaaa tctaagaatt
    12721 atagtttaac aacgtttgca gcttgtggac cgcggtcgcc ttcaactact tcaaactcaa
    12781 cagcttgacc ttcttctaat gatttgtaac catcttggtt aattgctgaa aaatgtacga
    12841 atacgtcatt ttctccttca acttcgataa agccgaatcc tttttcagcg ttaaaccatt
    12901 taactgtacc ttgtttcata atctgaaacc tccaagacta aaattcattc aatatgctta
    12961 ttcgcatatt acaaaaaata catgcaaatt tcactcaaat attctttgca atatggaata
    13021 atcatctgct taacttgtat tatagtgcag ttattaccaa attgcaatac aataataaaa
    13081 aatattcatt taatcaatta ttggtagttt ataatatact tcattatttt gtaaaacaat
    13141 gaaactataa aatatttgta aatcctcttc acaatcttcg caatagttca tgtcacaatt
    13201 attataaaat gcataaatat tatatttgcc tgtgacatag ttatcgaact gaactttaca
    13261 ttgattaatc aattgttgat agtgcttaaa catgtcatca aaatcttgat attgatattt
    13321 ttcaacaatg ttttctggcc agtcactgaa taaccaccat ccctcataat cggctctaat
    13381 tttggtaact gtccacatta ttaaagaccc cttccatact tcaaaaactc tatacttatt
    13441 tttatacctt tatcctctga aaatcaagtt tgacgacttg aaacaaacaa agtagtaact
    13501 atagtaatta ttagtttttt taagtaaaac tacgtataat aacattatga ggtggtaaaa
    13561 atgagacata tacatttaca agtattcgga cgcgttcaag gcgtcggatt tagatatttt
    13621 acacaacgca ttgcaatgaa ctataacatt gtcggtactg ttcaaaatgt agatgactat
    13681 gtagagatat atgcacaagg ggatgacgca gatatagaga gatttattca aggtgtaatt
    13741 gaaggtgcct caccagcatc aaatgtaaca agccatcaac ttgaagagtt agaactaaat
    13801 caaaaattat cggattttcg atcaatataa aatgctaaat aaaggaagat gacagtgaga
    13861 tataatattt ctcatatatt tggggtgtta gtgggaattc ctgtagcgtt tttaacaagc
    13921 atatttggga tgattgcact tgatgtatct tttttaattg atatgtctat tggtattgtt
    13981 ggctttttaa tgacatacct accgatacaa aaactcactt cacgcaaata tttaaacgaa
    14041 attggtttga ctagaaaaga ctatcgctat attcgaaatc agttaaatca tacacaccaa
    14101 aaacttagag gtattttaaa aacgtatgtc aatataagat caattaaaga ttttaggcag
    14161 attaatgata tataccaaat ttcacgttct atttatacga cagttagaca gagacctgca
    14221 tcattttata aagttgaagg ctttttttat tctcatattg ataatgcttt aaatttggtt
    14281 gatgcatata cacgtctagc aaaaatgccc aaaaaatcaa ttaatgaaca gcaaaagtta
    14341 gaacaaacac gaattacttt ggatgaggtc aaacgaacat taatcgctga tttaaagcgt
    14401 ctcaacgaag atgattatga acgtttagat attgaaatgg aattaaataa gttacatcaa
    14461 aaacatcatc aagattgatg taaaatagca actattggag agatgattga tgactgaaaa
    14521 taaaagtttc aaagaaagcc atccactaga tgattttata agcgataaag aattatcgaa
    14581 tactactatt caaaaagaaa agttaacaat tgaacaacaa aaacaggtag acacaatcag
    14641 taaacaaatt aaccctttag acaatgaagg tttattagcg tttggttctg atttacagaa
    14701 acaaatgtct caattttcac atcaaatgtt ggatgaagta caaagtaaag atgttggtcc
    14761 tattggagat actttgtcag atctaatgtc aaaactaaag tcagttaatc caaatgagtt
    14821 aaatactgat aaaccatcta tgttaaaaag aatttttagc agagcaaagt cgtctatcaa
    14881 tgaaatcttt tcaagaatgc aatcagttag tgctcaagtc gatcgcataa cgattcaact
    14941 gcagaaacat caaacacatt taacaagaga tattgaatta ttagatacgc tatatgataa
    15001 aaacaaacaa tactttgatg acttatcatt gcatatcatt gctgcacagc aaaaaaagtt
    15061 gcaattagaa aatgaaaagc taccacaatt gcaacagcaa gcgcagcaat ccactaatca
    15121 aatggatatt caacaagttg cagatatgca gcaatttata gatagactag ataaacgcat
    15181 atatgactta cagctttcaa gacaaatagc tttgcaaact gcgccacaaa ttcgtatgat
    15241 tcaaaatgtt aatcaagcac ttgccgagaa gatacaaagt tcaattttga caagtattcc
    15301 actatggaaa aatcaaatgg ccattgcgct tacattaatg agacagcgta atgcagttgc
    15361 tgcacaacga gctgtcactg atacaactaa tgatttatta acagcaaatg ctgaaatgtt
    15421 gaaacaaaat gcgattgaaa ctgcaacaga aaatgagcgt ggcattgttg atcttgatac
    15481 attgaaacgt acacagcgta acattattga gacaattgaa gaaacattaa ttattcaaca
    15541 acacggtcgc gaagaacgac aattagctga aaaagaatta caacaattag aacaagattt
    15601 aaagtcacat ttagtgaaca tcaaaggacc gaataaacaa tcataaatta cgaattagat
    15661 ataacaaaaa agagcttggg acattaagtt ccttaaagtt cttaggcaat gtaaaaaagc
    15721 tgatttctat ta
//
LOCUS       DANRDI010000049        15693 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.049, whole genome shotgun sequence.
ACCESSION   DANRDI010000049 DANRDI010000000
VERSION     DANRDI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.049"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..269)
                     /locus_tag="PZ377_001227"
     CDS             complement(<1..269)
                     /locus_tag="PZ377_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDM6358107.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRI"
     gene            971..1600
                     /locus_tag="PZ377_001228"
     CDS             971..1600
                     /locus_tag="PZ377_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDM6358108.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            2360..3373
                     /locus_tag="PZ377_001229"
     CDS             2360..3373
                     /locus_tag="PZ377_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDM6358109.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            3426..4436
                     /gene="gap"
                     /locus_tag="PZ377_001230"
     CDS             3426..4436
                     /gene="gap"
                     /locus_tag="PZ377_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDM6358110.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            4575..5765
                     /locus_tag="PZ377_001231"
     CDS             4575..5765
                     /locus_tag="PZ377_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDM6358111.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            5887..6648
                     /locus_tag="PZ377_001232"
     CDS             5887..6648
                     /locus_tag="PZ377_001232"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDM6358112.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            6651..8168
                     /locus_tag="PZ377_001233"
     CDS             6651..8168
                     /locus_tag="PZ377_001233"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDM6358113.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            8298..9602
                     /gene="eno"
                     /locus_tag="PZ377_001234"
     CDS             8298..9602
                     /gene="eno"
                     /locus_tag="PZ377_001234"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDM6358114.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            9942..10400
                     /locus_tag="PZ377_001235"
     CDS             9942..10400
                     /locus_tag="PZ377_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358115.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            10467..10700
                     /gene="secG"
                     /locus_tag="PZ377_001236"
     CDS             10467..10700
                     /gene="secG"
                     /locus_tag="PZ377_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDM6358116.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            10816..11556
                     /locus_tag="PZ377_001237"
     CDS             10816..11556
                     /locus_tag="PZ377_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDM6358117.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            11590..13962
                     /gene="rnr"
                     /locus_tag="PZ377_001238"
     CDS             11590..13962
                     /gene="rnr"
                     /locus_tag="PZ377_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDM6358118.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            13984..14448
                     /gene="smpB"
                     /locus_tag="PZ377_001239"
     CDS             13984..14448
                     /gene="smpB"
                     /locus_tag="PZ377_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDM6358119.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            14539..14900
                     /gene="ssrA"
                     /locus_tag="PZ377_001240"
     tmRNA           14539..14900
                     /gene="ssrA"
                     /locus_tag="PZ377_001240"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         5534 a         2343 c         3060 g         4756 t
ORIGIN      
        1 tgaattctac ttagcattag cgtttgttta tattccggtg tccatcgttt attcaatgta
       61 gcacctgcta agttgatgac cacatcgata ttttgaggaa ctttgtgttc ccacccagat
      121 ttagcccagt tgacatatga aattttctta tcatttgaaa tttggtcgtg tcgcgttaat
      181 atcgtgatat gtgaatctga ttttttaatt tcattaacta attgagatcc aaccatacca
      241 gtcccaccag taattaagta ttgtttcatt atcattcacc ccatgtaatt ttgtatttag
      301 ttactattta ataatcatta ttgttgttca aaggttatac attatttaga caataatatg
      361 tcaataactt tttttgaatt gtattttaat aaaaatgata taagttataa taaaggtgta
      421 ccttcgataa cgaataaaca tctcttaaaa gtatgtgtaa aacgctgcat gatacaaacg
      481 aaggtaaaaa tttgactccc tttagtagtg gacccgtacg ttaatcgtgc gggtcgtttt
      541 tatttatttc ttattcccat tatacatcaa tttaaagcat taatttttta aacaaattta
      601 agaatacata gtaatataac aatctaaaca taaaaacttt taacacaaca cttaaaccaa
      661 tgctttaatt ttcaatacgt agctataatt tgttgtaaaa tcaaaaaggt taaaatgtta
      721 attttcaaaa aaggctcaaa atatgtttga tttagttatt aaatgttaag atatataaga
      781 ctactatttc tttgtaaaaa tgaatccgat ttacgagtga gtaatagtga aggcagtttt
      841 aagttgaaga aggcaaaaag agtaaatgtt tattaatatt tgtagaaact aggtaagcaa
      901 attagttgtg aaaatgttaa tggttgcgtg ataatttcta tatttaaatt agtttgaagt
      961 gagggagagt atgtcgaatc aaaattacga ctacaataaa aatgaagatg gaagtaagaa
     1021 gaaaatgagt acaacagcga aagtagttag cattgcgacg gtattgctat tactcggagg
     1081 attagtattt gcaatttttg catatgtaga tcattcgaat aaagctaaag aacgtatgtt
     1141 gaacgaacaa aagcaggaac aaaaagaaaa gcgtcaaaaa gaaaatgcag aaaaagagag
     1201 aaagaaaaag caacaagagg aaaaagagca gaatgagcta gattcacaag caaaccaata
     1261 tcagcaattg ccacagcaga atcaatatca atatgtgcca cctcagcaac aagcacctac
     1321 aaagcaacgt cctgctaaag aagagaatga tgataaagca tcaaaggatg agtcgaaaga
     1381 taaggatgac aaagcatctc aagataaatc agatgataat cagaagaaaa ctgatgataa
     1441 taaacaacca gctcagccta aaccacagcc gcaacaacca acaccaaagc caaataataa
     1501 tcaacaaaac aatcaatcaa atcagcaagc aaaaccacaa gcaccacaac aaaatagcca
     1561 atcaacaaca aataaacaaa ataatgctaa tgataagtag tatttagtca aacaaaaatg
     1621 aaccagtatg acagacaaca caattaatta ggttgtctcg aatattggtt cttattttta
     1681 taattgttaa ttaggggaga gatgatactt aaatagttag ttatttattt tacggatagt
     1741 gaaatttatt ttgagtgagg tgggacagaa atgatatttt cgcaaaattt atttcgtcgt
     1801 cccaccccaa cttccattgc ctgtagaaat tggaaatcca atttctctat gttggggccc
     1861 caccccaact tgcattgcct gtagaaattg ggaggagcca atttcactat gttggggccc
     1921 caccccaact cgcattgcct gtagaatttc atttcgaaat tctctatgtt ggggcccctg
     1981 actttaattg aaaaaagctt gttacaagtg cattttcgtt cggttaacta ctactaatgt
     2041 gactttttgg attctagagc attgatttat gtcctagtct caaataataa gcaaatgatt
     2101 atcgagtcag tataagggta tacatttgac tacagcgaat aaaataaaac gttttgttga
     2161 ataacaaatc gaaaatatat tgcaagcgct ttatcaaatt atttagaaaa tttaagtttt
     2221 atgcttgcaa tttttgaaat agaatagtac tattgcaagt gtaaagaggt taatttttgt
     2281 cccacgcggg acttaaaaag gcaaccactg gttgtgacat atccttattt acatttataa
     2341 atataaggag gaggtagtag tgaaagactt attgcaagca cagcaaaagc ttataccgga
     2401 tctcatagat aaaatgtata aacgtttttc tattcttact actatctcaa aaaatcagcc
     2461 tgtcggacgt cgaagtttaa gcgaacatat ggatatgact gaacgtgtac tgcgttctga
     2521 aacagatatg cttaagaaac aagatttgat aaaagttaag cctaccggaa tggaaattac
     2581 agctgaaggt gagcaactga tttcgcaatt gaaaggttac tttgatatct atgcagatga
     2641 taatcgtctg tcagaaggta ttaagaataa atttcaaatt aaggaagttc atgttgttcc
     2701 tggtgatgct gataatagtc aatctgttaa aacagaatta ggtagacaag caggtcaatt
     2761 acttgaaggc atattacaag aagacgcgat agttgctgta actggcggat ccacgatggc
     2821 atgtgttagt gaagcaattc atttattacc atataatgta ttcttcgtac cagccagagg
     2881 tggactaggc gaaaatgttg tctttcaggc aaacacaatt gcagccagta tggcacaaca
     2941 agctggcggt tattatacga cgatgtatgt acctgataat gtcagtgaaa caacatataa
     3001 tacattgttg ttagagccat cagtcataaa cactttagac aaaattaaac aagcaaacgt
     3061 tatattacac ggcattggtg atgcgctgaa gatggcgcat cgacgtcaat cacctgaaaa
     3121 ggtcattgaa caacttcaac atcatcaagc tgtcggagag gcatttggtt attattttga
     3181 tacacaaggt caaattgtcc ataaggttaa aacaattgga cttcaattag aagaccttga
     3241 atcaaaagac tttatttttg cagttgcagg aggcaaatcg aaaggtgaag caattaaagc
     3301 atacttgacg attgcaccca agaatacagt gttaatcact gatgaagccg cagcaaagat
     3361 aatacttgaa taagagataa aaagtttaat actttttaaa tatcatttta aaggaggcca
     3421 ttataatggc agtaaaagta gcaattaatg gttttggtag aattggtcgt ttagcattca
     3481 gaagaattca agaagtagaa ggtcttgaag ttgtagcagt aaacgactta acagatgacg
     3541 acatgttagc gcatttatta aaatatgaca ctatgcaagg tcgtttcaca ggtgaagtag
     3601 aggtagttga tggtggtttc cgcgtaaatg gtaaagaagt taaatcattc agtgaaccag
     3661 atgcaagcaa attaccttgg aaagacttaa atatcgatgt agtattagaa tgtactggtt
     3721 tctacactga taaagataaa gcacaagctc atattgaagc aggcgctaaa aaagtattaa
     3781 tctcagcacc agctactggt gacttaaaaa caatcgtatt caacactaac caccaagagt
     3841 tagacggttc tgaaacagtt gtttcaggtg cttcatgtac tacaaactca ttagcaccag
     3901 ttgctaaagt tttaaacgat gactttggtt tagttgaagg tttaatgact acaattcacg
     3961 cttacacagg tgatcaaaat acacaagacg cacctcacag aaaaggtgac aaacgtcgtg
     4021 ctcgtgcagc ggcagaaaac atcatcccta actcaacagg tgctgctaaa gctatcggta
     4081 aagttattcc tgaaatcgat ggtaaattag atggtggtgc acaacgtgtt cctgtagcta
     4141 caggttcatt aactgaatta acagtagtat tagaaaaaca agacgtaaca gttgaacaag
     4201 ttaacgaagc tatgaaaaat gcttcaaacg aatcattcgg ttacactgaa gacgaaatcg
     4261 tttcttcaga cgttgtaggt atgacttacg gttcattatt cgacgctaca caaactcgtg
     4321 taatgtcagt tggcgaccgt caattagtta aagttgcagc ttggtatgat aacgaaatgt
     4381 catatactgc acaattagtt cgtacattag catacttagc tgaactttct aaataatttt
     4441 agtatagttt ttattcaaat acgctagtgc tcagaactat ttagcattaa ttaaagctta
     4501 tgagtaagcg gggagcacaa acgcttctcc gcttattttt atataaaatt tcctaattac
     4561 aaggaggaaa caccatggct aaaaaaattg tttctgattt agatcttaaa ggtaaaacag
     4621 tcctagtacg tgctgatttt aacgtacctt taaaagacgg tgaaattact aatgacaacc
     4681 gtatcgttca agctttacct acaattcaat acatcatcga acaaggtggt aaaatcgtac
     4741 tattttcaca tttaggtaaa gtgaaagaag aaagtgataa agcaaaatta actttacgtc
     4801 cagttgctga agacttatct aagaaattag ataaagaagt tgttttcgta ccagaaacac
     4861 gcggcgaaaa acttgaagct gctattaaag accttaaaga aggcgacgta ttattagttg
     4921 aaaatacacg ttatgaagat ttagacggta aaaaagaatc taaaaatgat ccagaattag
     4981 gtaaatactg ggcatcttta ggtgatgtgt ttgtaaatga tgcttttggt actgcgcatc
     5041 gtgagcatgc atctaatgtt ggtatttcta cacatttaga aactgcagct ggattcttaa
     5101 tggataaaga aattaagttt attggcggcg tagttaacga tccacataaa ccagttgttg
     5161 ctattttagg tggagcaaaa gtatctgaca aaattaatgt catcaaaaac ttagttaaca
     5221 tagctgataa aattatcatc ggcggaggta tggcttatac tttcttaaaa gcgcaaggta
     5281 aagaaattgg tatttcatta ttagaagaag ataaaatcga cttcgcaaaa gatttattag
     5341 aaaaacatgg tgataaaatt gtattaccag tagacactaa agttgctaaa gaattttcta
     5401 atgatgccaa aatcactgta gtaccatctg attcaattcc agcagaccaa gaaggtatgg
     5461 atattggacc aaacactgta aaattatttg cagatgaatt agaaggtgcg cacactgttg
     5521 tatggaatgg acctatgggt gtattcgagt tcagtaactt tgcacaaggt acaattggtg
     5581 tatgtaaagc aattgcaaac cttaaagatg caattacgat tatcggtggc ggtgattcag
     5641 ctgcagcagc aatctcttta ggttttgaaa atgacttcac tcatatttca actggtggcg
     5701 gcgcgtcatt agagtaccta gaaggtaaag aattgcctgg tatcaaagca atcaataata
     5761 aataataaag tgatagttta aagtgatgtg gcatgtttgt ttaacattgt tacgggaaaa
     5821 cagtcacaag atgacatcgt gtttcatcac ttttcaaaaa tatttacaaa acaaggagtg
     5881 tctttaatga gaacaccaat tatagctggt aactggaaaa tgaacaaaac agtacaagaa
     5941 gcaaaagact tcgtcaatgc attaccaaca ttaccagatt caaaagaagt agaatcagta
     6001 atttgtgcac cagcaattca attagatgca ttaactactg cagttaaaga aggaaaagca
     6061 caaggtttag aaatcggtgc tcaaaatacg tatttcgaag ataatggtgc gttcacaggt
     6121 gaaacgtctc cagttgcatt agcagattta ggcgttaaat acgttgttat cggtcattct
     6181 gaacgtcgtg aattattcca cgaaacagat gaagaaatta acaaaaaagc gcacgctatt
     6241 ttcaaacatg gaatgactcc aattatatgt gttggtgaaa cagacgaaga gcgtgaaagt
     6301 ggtaaagcta acgatgttgt aggtgagcaa gttaagaaag ctgttgcagg tttatctgaa
     6361 gatcaactta aatcagttgt aattgcttat gaaccaatct gggcaatcgg aactggtaaa
     6421 tcatcaacat ctgaagatgc aaatgaaatg tgtgcatttg tacgtcaaac tattgctgac
     6481 ttatcaagca aagaagtatc agaagcaact cgtattcaat atggtggtag tgttaaacct
     6541 aacaacatta aagaatacat ggcacaaact gatattgatg gggcattagt aggtggcgca
     6601 tcacttaaag ttgaagattt cgtacaattg ttagaaggtg caaaataatc atggctaaga
     6661 aaccaactgc gttaattatt ttagatggtt ttgcgaaccg cgaaagcgaa catggtaatg
     6721 cggtaaaatt agcaaacaag cctaattttg atcgttatta caacaaatat ccaacgactc
     6781 aaatcgaagc gagtggctta gatgttggac tacctgaagg acaaatgggt aactcagaag
     6841 ttggtcatat gaatatcggt gcaggacgta tcgtttatca aagtttaact cgaatcaata
     6901 aatcaattga agacggtgat ttctttgaaa atgatgtttt aaataatgca attgcacacg
     6961 tgaattcaca tgattcagcg ttacacatct ttggtttatt gtctgacggt ggtgtacaca
     7021 gtcattacaa acatttattt gctttgttag aacttgctaa aaaacaaggt gttgaaaaag
     7081 tttacgtaca cgcattttta gatggccgtg acgtagatca aaaatccgct ttgaaataca
     7141 tcgaagagac tgaagctaaa ttcaatgaat taggcattgg tcaatttgca tctgtgtctg
     7201 gtcgttatta tgcaatggat cgtgacaaac gttgggaacg tgaagaaaaa gcttacaatg
     7261 ctattcgtaa ttttgatgcc ccaacttatg caactgccaa agaaggtgta gaagcaagct
     7321 ataatgaggg cttaactgac gaattcgtag taccattcat cgttgagaat caaaatgacg
     7381 gtgttaatga tggagatgca gtgatcttct ataatttccg acctgataga gcagcgcaat
     7441 tatcggaaat ttttgcgaac agagcattcg aaggctttaa agttgaacaa gttaaagact
     7501 tattctatgc aacattcact aagtataatg acaatatcga tgcggctatc gtcttcgaaa
     7561 aagttgattt aaataataca attggtgaaa ttgcacaaaa taacaattta actcaattac
     7621 gtattgcaga aactgaaaaa taccctcacg ttacttactt tatgagtggt ggacgtaacg
     7681 aggaatttaa aggtgaacgc cgtcgtttaa ttgattcacc taaagttgca acgtatgact
     7741 tgaaaccaga aatgagtgct tatgaagtta aagatgcatt attagaagag ttaaataaag
     7801 gtgacttgga cttaattatt ttaaactttg ctaaccctga tatggttgga catagtggta
     7861 tgcttgagcc gacaatcaaa gcaatcgaag cggttgatga atgtttagga gaagtggttg
     7921 ataagatttt agacatggac ggttatgcaa ttattactgc tgaccatggt aactctgatc
     7981 aagtattgac ggatgatgat caaccaatga ctacgcatac aacgaaccca gtaccagtga
     8041 ttgtaacaaa agaaggcgtt acacttagag aaactggtcg cttaggtgac ttagcaccta
     8101 cattattaga tttattaaat gtagaacaac ctgaagatat gacaggtgaa tctttaatta
     8161 aacactaata ttgtaaaaga tgttaagtaa acgcttaatg acacttattt tttgaaaata
     8221 atagtaatat cattttgtta aatgaaagaa taaagctata ataattatag aataactatt
     8281 taaaggagat tataaacatg ccaattatta cagatgttta cgctcgcgaa gtcttagact
     8341 ctcgtggtaa cccaactgtt gaagtagaag tattaactga aagtggcgca tttggtcgtg
     8401 cattagtacc atcaggtgct tcaactggtg aacacgaagc tgttgaatta cgtgatggag
     8461 acaaatcacg ttatttaggt aaaggtgtta ctaaagcagt tgaaaacgtt aatgaaatca
     8521 tcgcaccaga aattattgaa ggtgaatttt cagtattaga tcaagtatct attgataaaa
     8581 tgatgatcgc attagacggt actccaaaca aaggtaaatt aggtgcaaat gctattttag
     8641 gtgtatctat cgcagtagca cgtgcagcag ctgacttatt aggtcaacca ctttacaaat
     8701 atttaggtgg atttaatggt aagcagttac cagtaccaat gatgaacatc gttaatggtg
     8761 gttctcactc agatgctcca attgcattcc aagaattcat gattttacct gtaggtgcta
     8821 caacgttcaa agaatcatta cgttggggta ctgaaatttt ccacaactta aaatcaattt
     8881 taagcaaacg tggtttagaa actgcagtag gtgacgaagg tggtttcgct cctaaatttg
     8941 aaggtactga agatgctgtt gaaacaatta tccaagcaat cgaagcagct ggttacaaac
     9001 caggtgaaga agtattctta ggatttgact gtgcatcatc agaattctat gaaaatggtg
     9061 tatatgacta cagtaagttc gaaggcgaac acggtgcaaa acgtacagct gcagaacaag
     9121 ttgactactt agaacaatta gtagacaaat atcctatcat tacaattgaa gacggtatgg
     9181 acgaaaacga ctgggatggt tggaaacaac ttacagaacg tatcggtgac cgtgtacaat
     9241 tagtaggtga cgatttattc gtaacaaaca ctgaaatttt agcaaaaggt attgaaaacg
     9301 gaattggtaa ctcaatctta attaaagtta accaaatcgg tacattaact gaaacatttg
     9361 atgcaatcga aatggctcaa aaagctggtt acacagcagt agtttctcac cgttcaggtg
     9421 aaacagaaga tacaacaatt gctgatattg ctgttgctac aaacgctggt caaattaaaa
     9481 ctggttcatt atcacgtact gaccgtattg ctaaatacaa tcaattatta cgtatcgaag
     9541 atgaattatt tgaaactgct aaatatgacg gtatcaaatc attctataac ttagataaat
     9601 aattttcttt ataatcaaat gctgacataa ttttagttga ggattattat gacggtataa
     9661 attaaataaa gattttgagt tcacgcttaa ataagttcac gcttaaattt atagcctgcc
     9721 acagagttga gactgtggta ggttttttat tttgaagtat taatcataac agactaataa
     9781 tcatgaggta actaataaca catatttaac ttgtattctt aaactggtat aataaattta
     9841 tgttgaaatg aatattgtat gacagggtat tcacttttat taaaaggtaa aattaaataa
     9901 aggttttata gaacgtattt aaatatatga ggagtaaaca aatggctgat agaacgaata
     9961 aagaaattaa aacaggacgc tttattgcaa ctgcatcaat cgtattctca atattattga
    10021 ttattcatta ctttgtttcg ttggataatg cgactgccaa agcattactt aatttaacga
    10081 atcaaaacac ttcagataaa gcgattgatt acattttaaa cagctttaga ttcactggta
    10141 ttatgtatat tttggcttat ctagcaggct tcatcacttt ttggaatcga catacttatg
    10201 tgtggtggtt tatgtttgca gtttatgtat caaatagttt gtttacgttg attaatttat
    10261 caatcacaat tcaagcaata aaagctgcac acggtgcgta cttaacattg ccaattttaa
    10321 ttgttattat aggttcggtt gcattagcga tttatatgct tgttgtttct atcaaacgta
    10381 aaagtacatt taatcgctag aaaattgatt ttaacaataa aaatatgata tactacttgt
    10441 cgtatataag gaacggagga caatttatgc atacattttt aatcgtatta ttaatcattg
    10501 attgtattgc attaataact gttgtactac tccaagaagg taaaagcagt ggactttcag
    10561 gtgccatcag tggtggtgct gagcagttat tcggtaaaca aaaacaacgt ggcgtcgatt
    10621 tattcttaaa tagattaaca attattttat caatattatt ttttgtactt atgatttgca
    10681 taagttatct tggtatgtaa ggtccggcga tgtaaatgtc gggctttttt atttataatt
    10741 aagaatgtaa tagtttaaca ataagctatg taaaatatat agcctagtta agtatgcaaa
    10801 gggagcgtta gatttatgca gataaaatta ccaaaacctt tcttttttga ggaaggtaaa
    10861 cgtgccgtgt tattactaca tggttttaca ggcaattcgt ctgatgttcg tcaattaggt
    10921 cgatttttac aaaagaaagg ttatacatca tatgcaccgc aatatgaagg ccacgcggca
    10981 ccaccagatg aaatactgaa atctagtcct ttcgtttggt ttaaagatgc gttagatggt
    11041 tatgattatc ttgttgaaca aggttatgat gaaattgttg ttgctggtct atcattaggt
    11101 ggggattttg ctttaaaatt aagcttaaat agagatgtaa agggtattgt aacgatgtgt
    11161 gctcctatgg gtggcaaaac tgaaggtgcc atttatgaag gctttttaga atatgcacgc
    11221 aattttaaaa agtatgaagg taaagatcaa gagactattg ataatgaaat ggatcatttt
    11281 aaaccaactg aaactttaaa agaactaagt gaagcattag atacgattaa agagcaagtt
    11341 gatgaagtgt tggatcctat tttagtgatt caagcagaaa acgacaatat gattgatcca
    11401 caatccgcaa attatatata tgaccatgta gattctgatg acaaaaatat caagtggtac
    11461 agtgaatctg gacatgttat tacgattgat aaagagaaag aacaagtatt tgaagatatt
    11521 tatcaatttt tagagtcatt agactggtca gaataaaaag agattttaac attagaaagg
    11581 aggggcataa tgaatttaaa gcaatctata gaagagatta ttaatcaacc tgaatatgaa
    11641 cctatgtcag tgtcagattt tcaagatgca ttaggtttaa gcagtgccga ctcgtttaga
    11701 gatttaatta aggtgcttgt ggagttagaa caatcaggat taatcgaacg tacaaaaaca
    11761 gacagatacc aaaaaaagca tagttataga ggtcaatcaa aattgataaa aggaacgtta
    11821 agtcaaaata aaaaaggctt tgcattctta agacctgaag atgaggatat ggaagatata
    11881 tttattcccc cgacgaaaat taatcgtgcc ttggatggag atactgttat tgtagaaatc
    11941 catcaatcaa aaggtgaaca taaaggtaaa atcgaagggg aagttaagtc gattgagaag
    12001 cattctgtaa ctcaagttgt tggtacgtat agtgaagcta gacattttgg ctttgttatt
    12061 ccggatgata aacgtattat gcaagatatt ttcattccta aaggtcaaag tttaggcgca
    12121 gtcgatggtc ataaggtact tgtacaaatt actaagtatg ctgatggttc agataatcca
    12181 gaaggacata tttctgctat tttaggacat aaaaatgatc ctggcgtaga tattttatct
    12241 attatctatc aacatggcat agaaattgaa tttcctgatg aagtgttaca agaagctgaa
    12301 gcagtacctg atcatattga aaatactgaa attaaaggcc gtcatgattt acgtgatgaa
    12361 ttgacaatca caattgatgg tgctgatgct aaagacttag atgacgcaat tagtgttaaa
    12421 aagttagcga acggtaatac gcaattaact gtaagtattg ctgatgtcag ctattatgta
    12481 acagaaggtt ctgcattgga taaagaggca tatgatagag cgacaagtgt atatcttgtt
    12541 gaccgtgtaa ttccaatgat tccacatcga ttaagtaatg gtatttgttc attgaatcct
    12601 aatgttgatc gtttaactct aagctgtcgc atggaaatcg atgctagtgg tcgcgttgtt
    12661 aaacatgaaa tttttgatag tgttatacat tctgattatc gaatgacgta tgatgcggta
    12721 aatcagatta ttactgaaaa ggatcctaac attcgcgaac aatataatga aattacgcct
    12781 atgctagatt tagcacaaga tttatctaat cgtttgattc aaatgagaaa acgacgtggt
    12841 gaaatcgatt ttgatattag tgaagcaaaa gtattagtta acgaagacgg tataccaaca
    12901 gatgttcaat taagacaacg tggcgagggt gaacgtctaa ttgaatcatt tatgttaatt
    12961 gcaaatgaaa cagttgctga acattttagt aagttagatg taccttttat ttaccgagtg
    13021 catgagcaac ctaaatcaga tcgcttaaga caattctttg attttattac aaactttggc
    13081 atcatgatta agggtactgg cgaagatatt catccaacaa cacttcaaaa ggttcaagaa
    13141 gaagtagaag gtcgacctga acaaatggtc atttcaacaa tgatgttgcg ttcaatgcaa
    13201 caagcgcatt atgatgatgt gaacttggga cattttggct tatcagctga atattatacg
    13261 cattttacat caccaattag acgttatcct gatttaacag ttcatcgttt aatccgtaag
    13321 tatttaattg agaaatcaat ggataacaaa gaagtgaagc gttgggaaga caaattgcct
    13381 gagttagctg aacatacttc taaacgtgaa cgtcgtgcta ttgaggcaga acgtgatact
    13441 gatgaattga aaaaagcaga atatatgatt caacatattg gtgatgaatt tgaaggtatt
    13501 gtcagctcag tagctaactt cggtatgttc attgaattgc caaatacgat agaaggtatg
    13561 gttcatattg cgaatatgac tgatgattat taccgttttg aagagcgtca aatggcatta
    13621 attggtgagc gtcaagctaa agtatttaga attggtgaca cagttaaggt taaagtgacg
    13681 catgttgatg tagatgaacg attaattgat tttcaaattg taggtatgcc tttaccgaaa
    13741 aatgatcgat cacagcgccc agcgcgaggt aagacaattc aagccaaaac gcgtggtaaa
    13801 tcattagata aatcaaaatc tgatgataag ggtcgtaaga aaaaaggtaa gcaacgtaaa
    13861 ggtaaaaacc aacgtaataa tgataaatca ggtaatagta agcataagcc attttataaa
    13921 gataaaagtg tgaaaaagaa agcacgtcgt aagaaaaaat aagcagcaat gaggtgagta
    13981 tgaatggcta agaagaaatc accaggtaca ttagcggaaa atcgtaaggc aagacatgat
    14041 tataatattg aagatacgat tgaagcggga attgtattgc aaggcacaga aataaaatca
    14101 attcgccgag gtagtgctaa ccttaaagat agttatgcgc aagttaaaaa cggtgaaatg
    14161 tatttgaata atatgcatat agcaccatac gaagaaggga atcgttttaa tcacgatcct
    14221 cttcgttctc gaaaattatt attgcacaag cgtgaaatca ttaaattggg tgatcaaaca
    14281 cgtgagattg gttattcgat tgtgccgtta aagctttatt tgaagcatgg acattgtaaa
    14341 gtattacttg gtgttgcacg aggtaagaaa aaatatgata aacgtcaagc tttgaaagaa
    14401 aaagcagtca aacgagatgt tgcgcgcgat atgaaagccc gttattaagc gatttagttg
    14461 cttaatcggg ctatatttga tatagttata tgtgcttttg taaattacaa aagtatgatt
    14521 tgtttgattt attatttcgg ggacgttcat ggattcgaca ggggtccccc gagctcatta
    14581 agcgtgtcgg agggttgtct tcgtcatcaa cacacacagt ttataataac tggcaaatca
    14641 aacaataatt tcgcagtagc tgcctaatcg cactctgcat cgcctaacag catttcctat
    14701 gtgctgttaa cgcgattcaa ccttaatagg atatgctaaa cactgccgtt tgaagtctgt
    14761 ttagaagaaa cttaatcaaa ctagcatcat gttggttgtt tatcactttt catgatgcga
    14821 aacctatcga taaactacac acgtagaaag atgtgtatca ggacctttgg acgcgggttc
    14881 aaatcccgcc gtctccatat ttgtagccta cagcctttgt ggttgtgggc ttttttattt
    14941 tgtgtttttc aggggataat gcattgcaga atttgttgtg agtattgata tagcagtgtt
    15001 tgtataggtg tttatttgat ggaggaaaga gtaataagtg attatgaatt agtttttgag
    15061 atataagggg acagtgatgt gtgtcaaata agtgtcaaaa aagttggatt ctgagtttta
    15121 cattcaacat tgttcatgaa gaaacttctt tatacgcaaa aaattctcca tgttatatat
    15181 gtcaatataa aaatgtgaat cgtctacact taattggata aatggctact gaaaaagaac
    15241 ttttcatttt tgttacgtca ctaagtgggt gtagttataa agagatgagc cgagttttga
    15301 tattttcatt agaatcaata tgcctattaa cacaatcagc aatagttgac gagacggaaa
    15361 taaaagaagt cgtagttaag aaatgcattt cacaacatac cattgtagcc atttttattg
    15421 ttttggatga taaactcttt ttggaatttt tagtttttat aatttgcaac tacactactt
    15481 cttttactaa tattaatgtc taagtaatcg ataaaaaatt ttccattgaa taaatgagaa
    15541 gttaaaaact ttacttaacc tttctcattg cattttccta ttcacgattt taagaaccca
    15601 acatactaca aacgaatttt aaaaggcgag agtaaagttt acttgtttat tatacatatt
    15661 taaaatccaa gagtcagaac agactactcc tct
//
LOCUS       DANRDI010000050        15543 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.050, whole genome shotgun sequence.
ACCESSION   DANRDI010000050 DANRDI010000000
VERSION     DANRDI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.050"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(7..459)
                     /locus_tag="PZ377_001241"
     CDS             complement(7..459)
                     /locus_tag="PZ377_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDM6358120.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(472..1236)
                     /locus_tag="PZ377_001242"
     CDS             complement(472..1236)
                     /locus_tag="PZ377_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDM6358121.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            complement(1785..2537)
                     /locus_tag="PZ377_001243"
     CDS             complement(1785..2537)
                     /locus_tag="PZ377_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDM6358122.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            2705..3691
                     /gene="pta"
                     /locus_tag="PZ377_001244"
     CDS             2705..3691
                     /gene="pta"
                     /locus_tag="PZ377_001244"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDM6358123.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            3694..4530
                     /locus_tag="PZ377_001245"
     CDS             3694..4530
                     /locus_tag="PZ377_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDM6358124.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            5117..6037
                     /gene="mvk"
                     /locus_tag="PZ377_001246"
     CDS             5117..6037
                     /gene="mvk"
                     /locus_tag="PZ377_001246"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDM6358125.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            6042..7025
                     /gene="mvaD"
                     /locus_tag="PZ377_001247"
     CDS             6042..7025
                     /gene="mvaD"
                     /locus_tag="PZ377_001247"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDM6358126.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            7038..8114
                     /locus_tag="PZ377_001248"
     CDS             7038..8114
                     /locus_tag="PZ377_001248"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDM6358127.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            8291..8632
                     /locus_tag="PZ377_001249"
     CDS             8291..8632
                     /locus_tag="PZ377_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDM6358128.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(8684..10006)
                     /locus_tag="PZ377_001250"
     CDS             complement(8684..10006)
                     /locus_tag="PZ377_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDM6358129.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(9993..10433)
                     /locus_tag="PZ377_001251"
     CDS             complement(9993..10433)
                     /locus_tag="PZ377_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDM6358130.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            10491..10592
                     /locus_tag="PZ377_001252"
     CDS             10491..10592
                     /locus_tag="PZ377_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358131.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            11058..12467
                     /locus_tag="PZ377_001253"
     CDS             11058..12467
                     /locus_tag="PZ377_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDM6358132.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            12471..13097
                     /locus_tag="PZ377_001254"
     CDS             12471..13097
                     /locus_tag="PZ377_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDM6358133.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
     gene            13223..13864
                     /locus_tag="PZ377_001255"
     CDS             13223..13864
                     /locus_tag="PZ377_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDM6358134.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNC
                     VIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIFPSFKNLCFVLFSYFFCGG
                     LSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            13952..14569
                     /locus_tag="PZ377_001256"
     CDS             13952..14569
                     /locus_tag="PZ377_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDM6358135.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            14644..15273
                     /locus_tag="PZ377_001257"
     CDS             14644..15273
                     /locus_tag="PZ377_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDM6358136.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            15330..15446
                     /locus_tag="PZ377_001258"
     CDS             15330..15446
                     /locus_tag="PZ377_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358137.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
BASE COUNT         5540 a         2165 c         2823 g         5015 t
ORIGIN      
        1 aaatatttat aatcgtttaa aagatttcac gaacccagaa acaattaatt tggaaatttg
       61 gtcggcgaat aataaaccta atgcgatggc gcctgcaata agtgtaacct ctagcatggt
      121 attgattgct gtactgaaat ttaataagac taaatttttt gtagcatcgt atgctaagcc
      181 accaggtact aatggaatga tacccgttac cataaaaatg atggcaggtt ctttttgttt
      241 acgagccata taatgactta acaagcctaa tgctaaacta ccaaagaaac tagagtatat
      301 agtgtgcaca ttaaagccgt tgaagaataa ggtgtaaacc atccatccac acgtaccaac
      361 gaaaccacat gatagatata attttctagg tgcatcaaaa atgacgcaga agaacattga
      421 agctaaaaag ctaaagataa agtttaagat ccaaaacata gtctgatact cctatactaa
      481 aattaatacg ctaccaacgc cagcaccgat gccaaacgca gtaaccaatg cttctaatga
      541 tttcgttgtg aacatcaaca tgtgtccacc aaataaatct tgtattgcgt ttgttattaa
      601 tacaccagga acaataggca tgactgccgc aatgataata gttgccaagt cacctgttgg
      661 aataagtgta tgtccaataa cggcgataat cccaataact aatgaaccaa tgaattctgg
      721 gataaactgt gcgtgtaact tacgatctaa aatctcagtg actaggtatc ctagactacc
      781 tgctaatatc gcagttaaaa catcaatcaa tctaccacct tgtaaatata agaaactcat
      841 tgcaatcatt gctgcagcaa aacctttaaa gggaagactg ctgtcacgct tagcaacata
      901 tattttttca agttgcgttt ttgcttcggc taaagaaatt tcattgtttg taatttgacg
      961 cgaaatttta ttagcttgag aaatttttat taagtttgta tctcgagagg taattctaaa
     1021 tattctagga aacgattccg aatgtaacgt aaactggatg acagtgtttg taacaaagct
     1081 gttactttca ctgtaaccaa gtttttttgc aatacgtgtc atggtatctt ctacacgcgt
     1141 accttctgca ccagattcta atagtatgcg agcagcaagc atgacaacgt ctttgataag
     1201 tacctcttgt ttgtattctt ctgaatttat gtccatttca tcaccattgt ttataagaat
     1261 ttaatactca ttatagttta tacactataa aataaccaca tgagcttttt gataagttgt
     1321 tattgatttt aaaaaactaa tatttgatac tattgtaata ataatcatcg gaatttttag
     1381 tagttaaaat tctcttgaag tataaaaagg aaaaattatt gaaaaaaatt cgacaaaaat
     1441 atgtttcaaa tatttcaaat aggttaaaat aatattagtt aaccattaca aaaattgtat
     1501 agagtagcga ctgtataatt tctattgagg ttaacgttta tatgtagtga tagtagttaa
     1561 agttctccca aggaagacta ctcgggtaca ctttgctatg agcaaagtgt actttgttat
     1621 tgataataca ttagcacata tatataagtt taaacataca gatttcaact atttaccaaa
     1681 tcacgtctta tgtatacggg aatatactga agataaacga aaaattccaa gcttaaaccg
     1741 ataagcttgg aattttttta ttaatttata aacgtaccaa tgtattaaga aatcgcaaag
     1801 aattgatcga attcgtttgt gttaataata tgtcctacaa agaaactacc gaattcaccg
     1861 tatcgtgctg ttgtttcatc aaagcgcatt tcgtatacaa tttttttgaa ttgtaatacg
     1921 tcatctgaga acaatgttac gccccattcg aaatcatcaa accctacaga accagtaata
     1981 aattgtttga ttttgccagc atattttcta ccaatcatac catggtcata cattaatttt
     2041 tggcgttctt ccatagttaa catgtaccag ttataagttt cattacgacg tttgttcatt
     2101 ggatagaaac aaatataatc agaatgtggt aattctgggt ataatcttgc tttgatatga
     2161 gggttctcat aaggatcttc atcagattta ccagctaaat aattgctcaa ttcaatgact
     2221 gatacatatg aatatgtagg gattaggaag tcagcaatgc gcaatttgtt aaattcattt
     2281 tcaatatgat ttaaagactt catttcagga cgtaagaacc ataataacaa atctgctttt
     2341 tgaccagtta tattataaat agcttgatca ccagattttg atgatcttac agttgctgta
     2401 ttttctaaaa atgattgaaa ttcagtgaca agtgcatcgc gttcgtcctt tggaactata
     2461 cgtaatgatg cccaatcaac tgcataaaat aaatgtagac tataccaacc atctaatgtt
     2521 tcggctgctt gactcatgtt tatcgctcct tttcaaaaat catcagttct ttataaattt
     2581 tatcataaag caaaggggta acgataaaat tatgattaca aattggtgac gtggcattat
     2641 gaaataaaat ggcgtataat tataccgtga atgattaata agatttatat tacaggagga
     2701 cattatggct gatttattaa atgtattaaa agacaaactt tctggtaaaa acgttaaaat
     2761 cgtattacct gaaggagagg acgaacgtgt tctaacagct gcaacacaat tacaagcaac
     2821 agattatgtt acaccaatcg tgttaggtga tgagactaag gttcaatctt tagcgcaaaa
     2881 acttgatctt gatatttcta atattgaatt aattaatcct gcgacaagtg aattgaaagc
     2941 tgaattagtt caatcatttg ttgaacgacg taaaggtaaa gcgactgaag aacaagcaca
     3001 agaattatta aacaatgtga actacttcgg tacaatgctt gtttatgctg gtaaagcaga
     3061 tggtttagtt agtggtgcag cacattcaac aggcgacact gtgcgtccag ctttacaaat
     3121 catcaaaacg aaaccaggtg tatcaagaac atcaggtatc ttctttatga ttaaaggtga
     3181 tgaacaatac atctttggtg attgtgcaat caatccagaa cttgattcac aaggacttgc
     3241 agaaattgca gtagaaagtg caaaatcagc attaagcttt ggcatggatc caaaagttgc
     3301 aatgttaagc ttttcaacaa aagggtctgc taaatcagac gacgtgacaa aagttcaaga
     3361 agctgtcaaa ttagcacaac aaaaagctga agaagaaaaa ttagaagcaa tcattgatgg
     3421 cgaattccaa tttgatgctg cgattgtacc aggtgttgct gagaaaaaag cgccaggtgc
     3481 taaattacaa ggtgatgcaa atgtctttgt attcccaagt ttagaagctg gtaatattgg
     3541 ttacaaaatt gcacaacgtt taggtggata tgatgcagtt ggtccagtat tacaaggttt
     3601 aaattctcca gtaaatgact tatcacgtgg ctgctcaatt gaagatgtat acaatctttc
     3661 aattattaca gcagcgcaag ccttacaata acgatggatt tagcgagtaa atattttaat
     3721 ggcgtcaact ggcgatatat cgatcattct tctggattag aacctatgca atctttcgca
     3781 ttcgatgata cattttgcga aagtgtgggc aaagatatat cagataatgt tgtgcgtact
     3841 tggattcatc aacatactgt tattcttggt attcatgatt caagattgcc gtttttaaaa
     3901 gatggcattg attatttaac gaatgagatt ggttataatg ccattgttag aaattctggt
     3961 ggcttaggtg tcgttctaga tcaaggtgta ttaaatatat cgctgatgtt caaaggacaa
     4021 acagaaacaa cgattgatga agcgtttact gtgatgtacc tcttaattag caaaatgttc
     4081 gaaaatgaga atgttgatat tgatacgatg gaaattgaac attcttattg cccaggaaaa
     4141 tttgacttaa gtatcgatgg taagaaattt gcaggcatat cgcaacgaag agttagaggc
     4201 ggtattgctg tacaaattta tctttgtgtt gaaggctctg gttcagaacg tgcattgatg
     4261 atgcaaacat tttatgaaca tgctttaaaa ggtgaagtga ctaaatttaa atatcctgaa
     4321 attgaaccat cttgtatggc ctcattagag acattgctta acaaaacgat tactgttcaa
     4381 gatgtaatgt ttttactatt atatgcaatc aaagatcttg gcggtgtatt aaatatgacg
     4441 ccaattactc aagaagaatg gcaaagatac gatacgtatt ttgataaaat gattgaaaga
     4501 aacaagaaaa tgatagatca aatgcaatag ttaaatattt aacagcccct ttcaagttat
     4561 cgtttggggc tgttattttt gaaagtatcc ataaaaagca taaatatgtt attgacctaa
     4621 tatttaaatt gaacatcatt aaattaaaag taatgatatg atggaaataa taaatttaat
     4681 aagaaaattt catgttttac gtattaaata gattgagaat agggtatata taatgcgtat
     4741 gactatgaaa gaaacgacct tctattgaaa tgttctaata ctttagtagt aaatctatat
     4801 attccaatta ttatttgtag tattatagtt agggtggaaa attataaatt gtagggctat
     4861 tttataaaaa gataaagaat ctgaaatatc actcgataag atttaaactt tgaagtaaac
     4921 gaaagttagt tttatactaa tcaagttaag tcaaatataa attcaaaata aagattatac
     4981 ataatatgaa taatgtgaga taaagactgc aatgaaattt aacgagaatg cgtatgcaac
     5041 ttagaatatg atgtctttta ctaataaata aaaataatga atcaagtata ggataaatat
     5101 aaaaggtgat attgacatga caagaaaagg atatggggaa tcgacaggta agattatttt
     5161 aataggagaa catgctgtta catttggaga gcctgctatt gcagtaccgt ttaacgcagg
     5221 taaaatcaaa gttttaatag aagccttaga gagcgggaac tattcgtcta ttaaaagcga
     5281 tgtttacgat ggtatgttat atgatgcgcc tgaccatctt aagtctttgg tgaaccgttt
     5341 tgtagaatta aataatatta cagagccgct agcagtaacg atccaaacga atttaccacc
     5401 atcacgtgga ttaggatcga gtgcagctgt cgcggttgct tttgttcgtg caagttatga
     5461 ttttttaggg aaatcattaa cgaaagaaga actcattgaa aaggctaatt gggcagagca
     5521 aattgcacat ggtaaaccaa gtggtattga tacgcaaacg attgtatcag gcaaaccagt
     5581 ttggttccaa aaaggtcatg ctgaaacgtt gaaaacgtta agtttagacg gctatatggt
     5641 tgttatagat actggtgtga aaggttcaac aagacaagca gtagaagatg ttcataaact
     5701 ttgtgaggac cctcagtaca tgtcacatgt aaaacatatc ggtaagttag ttttacgtgc
     5761 gagtgatgtg attgaacatc ataactttga agccttagcg gatattttta atgaatgtca
     5821 tgcggattta aaggcgttga cagttagtca tgataaaata gaacaattaa tgaaaattgg
     5881 taaagaaaat ggtgcgattg ctggaaaact tactggcgct ggtcgtggtg gaagtatgtt
     5941 attgcttgcc aaagatttac caacagcgaa aaatattgta aaagctgtag aaaaagctgg
     6001 tgcagcacat acttggattg agaatttagg aggttaatgc gttgattaaa agtggcaaag
     6061 cacgtgcaca tacgaatatt gcacttataa aatattgggg taaaaaagat gaagcactaa
     6121 tcattccaat gaataatagc atatctgtta cattagaaaa attttacact gaaacgaaag
     6181 tcacttttaa cgaccagtta acacaggatc aattttggtt gaatggtgaa aaggttagtg
     6241 gcaaagaatt agagaaaatt tcaaaatata tggatattgt cagaaataga gctggcatcg
     6301 attggtatgc agaaattgaa agcgacaatt ttgtaccaac agcagcaggg ttggcttcat
     6361 cggcaagcgc atatgcagct ttagcagcag cttgtaatca agcgctagac atgcagctgt
     6421 cagataagga tttatcgaga ttggcgcgaa ttggttcggg ttctgcgtcg cgtagtattt
     6481 atggtggatt tgcagaatgg gaaaaagggt atagtgatga gacgtcatat gccgttccac
     6541 ttgaatcgaa tcattttgaa gatgaccttg ccatgatatt tgttgtgatt aatcaacatt
     6601 ctaaaaaggt acctagtcga tatggtatgt cattgacacg aaacacatca aggttttatc
     6661 aatattggtt agatcatatt gatgaagatt tagctgaagc aaaagcagcg attcaagaca
     6721 aagattttaa acgccttggt gaagtaattg aagaaaatgg tttgcgtatg catgccacga
     6781 atctaggatc aacaccgccg ttcacatatc ttgtgcaaga aagttatgat gtcatggcgc
     6841 ttgttcacga atgccgagaa gcggggtatc cgtgttattt tacaatggat gcgggaccta
     6901 atgtgaaaat acttgtagaa aagaaaaaca agcaacagat tatagataaa ttattaacac
     6961 agtttgataa taaccaaatt attgatagtg acattattgc cacaggaatt gaaataattg
     7021 agtaaggaag agataaaatg attcaggtca aagcacccgg aaaactttat attgctggag
     7081 aatatgctgt aacagaacca ggatataaat ctgtacttat tgcgttagat cgttttgtaa
     7141 ctgctactat tgaagaaaca gaccaatata aaggtaccat tcattcaaaa gcattacatc
     7201 ataacccagt tacatttagt agagatgaag atagtattgt catttcagat ccacatgcag
     7261 caaaacaatt aaattatgtg gtcacagcta ttgaaatatt tgaacaatac gcgaaaagtt
     7321 gcgatatagc gatgaagcat tttcatctga ctattgatag taatttagat gattcaaatg
     7381 gtcataaata tggattaggt tcaagtgcag cagtacttgt gtcagttata aaagtattaa
     7441 atgaatttta tgatatgaag ttatctaatt tatacattta taaactagca gtgattgcaa
     7501 atatgaagtt acaaagttta agttcatgcg gagatattgc tgtgagtgta tatagtggat
     7561 ggctagcgta tagtactttt gatcatgaat gggttaagca tcaaattgaa gatactacgg
     7621 ttgaagaagt tttaatcaaa aactggcctg gattgcacat cgaaccatta caagcacctg
     7681 aaaatatgga agtacttatc ggttggactg gctcaccggc gtcatcacca cactttgtta
     7741 gcgaagtgaa acgtttgaaa tcagatcctt cattttacgg tgacttctta gaagattcac
     7801 atcgttgtgt tgaaaaactt attcatgctt ttaaaacaaa taacattaaa ggtgtgcaaa
     7861 agatggtgcg tcagaatcgt acaattattc aacgtatgga taaagaagct acagttgata
     7921 tagaaactga aaagctaaaa tatttgtgtg atattgctga aaagtatcac ggcgcatcta
     7981 aaacatcagg cgctggtggt ggagactgtg gtattacaat tatcaataaa gatgtagata
     8041 aagaaaaaat ttatgatgaa tggacaaaac atggtattaa accattaaaa tttaatattt
     8101 atcatgggca ataaatgatt tgatagggga gcaatcatct aatttaatta gaagataaat
     8161 gctccatttt ttattgtcag ccgtgttacg tatcttgaaa aaaatattaa aaagagtaaa
     8221 atagatagag ttaacgaaaa aattaatgaa atcgacgata tagatatgat aaagaaaggt
     8281 gggtagcaat atgaaaaata cattccttat ttgtgatgaa tgtcaggcag tcaatataag
     8341 aacgttacaa aagaagttgg aaaaattaga tcccgatgct gaaatcgtga taggttgtca
     8401 atcttattgt ggacctggac gccgaaaaac attcactttt gttaataacc gcccactggc
     8461 tgcgcttact gaagaagaat taatcgaaaa agtttctcaa caattaaaga aaccacgtga
     8521 tcctgaagaa gaagagcgtt taagaaaacg acatgaagaa cgtaaacgtc gtaaagtaga
     8581 acaagataga aagcttaaag aaaaattaga aaagcgaaaa gcacaacaat aaagcctgat
     8641 ggcagcatca ttcaatgcgt gccaccaggt ttttatgttt tgtctagaaa ttaaataaat
     8701 cattaaatga ttcggccatc gtaggatgcg tataaatatt atctcgtaat acggtatatg
     8761 gaatgttttg atcaatcgca agtttaatta tattaattaa ttcttcagat tgcttaccat
     8821 ataatgtagc acctaaaatc atattatttt cattattaat gactacttta aataaacctc
     8881 ttggatcatt gttaatttta tgacgaggta tagcacttac taaaagttga tgttcagtgt
     8941 aatcataatg ttgagcggca gcttctttac tagttaatcc aacacgtgat aatggtggat
     9001 ctataaatac tgtataaggc acgctgcctc tattgtcagt cgtacgtgac tgattaccat
     9061 ataacgctga tttgataatt cgataatcat ctaaagatat atacgtaaat tgaagtccgc
     9121 ctttaacatc acctgcagca taaatatgcg gcacagttgt ttgaagatga gcattgactt
     9181 taatttcgcc tctgtcgcct aattcgatat cagtattttc taaagctaaa tccgtattcg
     9241 gtttgcgccc gatagccaaa agtactgcat cagcctcaaa gttatcaacg ttggtatgga
     9301 ctgttgtatg atgattgtca gatgacaatt cagtcgtttc aacatttgta tgcaatgcaa
     9361 tgcctttatt ttctaagtca gtaataccat atgcaacgac atcttgatct tcgcgtggca
     9421 taaatgattc gccacgttct aatactgtta ccttactacc taaattcgca aacattgaag
     9481 caaattctaa ggcgatataa ccgccaccta caataacgag gtgcttaggt tgatagctaa
     9541 tgtttaataa acctgtcgaa tcgaagacgt gtttagcttg atcaaggcct ttaatgttag
     9601 gaatgacaga ggtagcaccg gtattaataa tgatatgagg tgcagtaata ctatcgacga
     9661 tatcgtcatg ttgatctaat aaattcactt cagtattaga tttaaactgc gctttaaaat
     9721 ccagtacatc aatgttgtta tcgtctgcta ataagtggta atttttattg tttagcgcat
     9781 tgacaacatc gtttttacgg ttataacttg cttcaaaaga tttgccttct aatccatcat
     9841 gtacaagtgt cttcgaaggt atacatccta tgtttataca agtgcctcca tacattttcg
     9901 gagattgttc gataactgcg acgtgttgac ctgttgatgc agcgtatttc gctaaagttt
     9961 taccagcttt cccaaatcct attacaatta aatcatatgt tttcatgaca taaatcctcc
    10021 ttttgaatgt cttcaatgac atctttgatt gtttttccat tataaaaatt aataatgata
    10081 ttctgttcgt cttgctgata atgtgacatg gtagttgcaa tattacgagc aatttgacag
    10141 tgactgcctt cgtcgccagt aaatagacgt gtgtggtgtt ctttctctaa gacaaaatgt
    10201 ttatataatg ttgctagaga gacatcagca ctttgatcat ttgctaaata accgccatct
    10261 ttacctcgta ttgtgtcaat catttttaaa tcgacaagtt gagtcgtcac gcgtcgtaat
    10321 tgaacaggat ttaaacaagt taattctgct aatgaactac tattgaattt ttctgaatga
    10381 tgcttagtta aaaaagctaa tacatgcacg gcaatgttaa attctaaatt caatgtttct
    10441 cacctcataa ttgtaactgt attagttaca attaaaaatc attttgctca atgtgtcaat
    10501 acttatgttt gaaaagtgtt gaattaggtg tggttattgt gatgatttat tgtttgaaag
    10561 ttgatgcatt aacggtgaga ttgcacaatt gaaattttaa ttatgtatga actgatagat
    10621 atgaaatgaa tattgaaaat gagagcaata aaattttgaa tgatgatgtt gtgtgattgt
    10681 ataaacttat tcgtatttta gatacgtttt gatatttcgc atgatgagga aatactttta
    10741 acaactgatg acatatttta atagtgaata tattttgaga tattaatata tttaatgtta
    10801 aggatgttgt cgcaataaaa ttaaaatatt gaaagataat ttttattgat taattgcgaa
    10861 tatacaaaga caccattata tccatatgct aaaataagaa aaaaggcgac atgttatctc
    10921 aatttacctg catctagact cctttacata agaaataatc atcgccgagt tttaaaattt
    10981 tacaactttg tagatgtaaa aagcatttga ttttaaatag tttgaaaatc atatattttt
    11041 gaaaaaggag catgccgatg agtattgaca tgtatttaga cagatctcga aaccaagctt
    11101 caagtgtggg gaatttgagt caaacaatga attcaaatta tgatgcgttg gaaaaagcaa
    11161 ttactcaatt tattaatgat gatgcgctta aagggaaagc gtatacgtca gctaagcaat
    11221 tttttagtac ggtgttaatt ccattatcaa caagtatgaa aacattgagt gatttaacga
    11281 agcaagcttg cgataatttt gtgtcacgtt atacgagtga ggttgatagc atatctttaa
    11341 aagaatcaga gcttgaagaa gatatcagat cattaagtca acaaattacg cgatatgaaa
    11401 atttgaataa caatttgaaa aagcatgctt ccgataatca gcaagccatt tcatcgaacc
    11461 aacaaataat acgaacatta ggtcaacaaa aacatgaatt agaagagaag ctacgcaaat
    11521 tgcgtgagtt taatcaaaaa tcaccagaaa tatttaaaga agttgaagaa tttcaaaaaa
    11581 ttgtccaaca aggacttacc caagcgcaga atttttggaa cttttcaaca aatcaattta
    11641 atattccttc aggtaaagaa cttgattggg ccaaagcaag tcatgaaaaa tatttgaaag
    11701 ttgctatggg gaaaattgaa cataaagcag agaaagaaac tttaaataaa gcagactttg
    11761 ctgttataaa ggcatatgcc aaagaacatc cagaagacga tatcccgaaa agtatattga
    11821 aatatataaa tgacaataaa gacagtatta aaagagatat aggattagat attacgtcaa
    11881 cacttttaga gcaaggcggt ataaatgcaa gtaaattcgg tgtatttatc aatacagcag
    11941 gtggagtgaa aggcccagca ggtccaaatt catttgtgga agtcaaacgt acatcaggta
    12001 atgtgtttat agaaaatggt agtaaatttg caaaaggcgg aaaataccta ggtaaaggtg
    12061 ttgctggtgt aggatttggt ataggtatgt atgatgacct tgcaaatgat gataaaacat
    12121 ttggagaggc gttgtcgcat aatggtatga cgcttgcagc tggatctgca gggacagcag
    12181 ttggagctgg attagcgact tttgttttag gaagtaatcc agtaggatgg gtgattttag
    12241 caggtttggc tatgagtaca gtatttgcat taggaacaga tttaatttat caaaataata
    12301 tttttggatt aaaagataaa gtagactggg tagggcataa aatcgataat agtatagatg
    12361 ttgttaaaaa aactacagaa aaatccatgg atagtgttgg gaatgctgtt agtgaagcta
    12421 aaaatattat tagcaatcat ataaatccaa tgaaatgggc gtggtgagtt ttgcttttta
    12481 atattaaggt tattaataaa aatccgagat ataaagtcgt tcaatataat gatgagtatt
    12541 tgttaattga tttagtaagc acttggcttg tatacttttt tccttttatt aattggttca
    12601 ttcccaaaag gtatgcaaaa cttagtgaga aggaacttga aaatttaaat gttgataaac
    12661 aaaataaaaa taatattttc tggccagtta ctggtagttc gtttttattt gtagttattt
    12721 tacggaaata tgtacatacg tttgaagttc aattagataa taagatttta atatctttat
    12781 gttttatagg atttataggt attgcagcat tttacattta cttaaataaa aagcttaaac
    12841 taaagatata tgatgataat ctagataatg aaaatagggt tatattagtg cctactttta
    12901 aagatggaag ttttatagtt ttcacatatc tattattagg aggttgttct atattatttt
    12961 taatatggct aatgactata aaacctcaaa atctactggt atttattatg tggattatta
    13021 ttacaatttt tttctttcta ataagcatgg gctcaattag taataaaaag gtttatgcca
    13081 aacttaaaaa gcaatagaaa ttactaaact tagattgtag ttcgtaagtt aagtaataaa
    13141 caggaaaata gtatgttact aaaatacaaa acacttataa agtatctgaa agtgtattag
    13201 atataaaacc aacgagacgg aagtggagat attgctttgc gaggttagag ttgtaaacaa
    13261 aaatcccaga tatagaatta ttaaatataa aaacgactat ctgatgattg atttagtaag
    13321 cacctggtta gttttgttct ttccgtttat taattggtta ataccaaaaa aatatgtcca
    13381 aatcagcaga gaagattttg ataatttaaa tattgtcaaa ccagttaaaa ataaagcttt
    13441 atggccagct ataggtagca tacttttatt tggcactatg tttagagata aaatatatat
    13501 acctgattct catttagaaa aaaattgcgt tattatcatt tgttcggttt tattattaag
    13561 tattctagtg ttttatatat atttaaatca gaaagtaaag ttatctatct ataatgatcg
    13621 gagtagcaat ggaaaaatta tgatttttcc atcattcaag aatctttgct ttgtactatt
    13681 ttcttatttt ttctgtggtg gattatcaat catgttctta gatgttttga ttagtttatc
    13741 tatccaaaat attatagtat ttattgcttg ggttattatg acaatgctat ttttctttat
    13801 aaatatgtct tcaataatag acaaaaagat tcatgttata tatttaaggt catataaata
    13861 ttaattttga tacaaaaggg cacaagtgtt ttgagaaaca gaaacgataa tatagctgtg
    13921 tgaatttgtt attaatagga agtgataatt attgctttgt gaaaccgaaa ttatcaataa
    13981 aaatccgaaa tacagagtta ttaagtatga tgatgagtat ttaatggtcg atgtaataag
    14041 aacttggctt gtatactttt ttccatttat taattggttc attccaaaaa ggtgcgccaa
    14101 aattagtaga gaagagtatg aaaaactgaa cactgttaag ccagttaaaa ataaaaattt
    14161 ttggcctgtt gtaggaggaa caatcctact aggtgctact tccagaaagt acatacactt
    14221 acttaatatt caattagaaa aaagatcggt tatatttata tgcttcgttg tatttctatg
    14281 tattttaatc ttttttgtgc ttctgaatcg aaaattaaaa ttgaaagttt tcgataataa
    14341 aaaagaagaa caaaaaataa ttttagtacc aacattaaaa aatgctgttc ttatattata
    14401 tggctatttg ttgattggag gcatgtcaat attagcttta agcatgttgc tgacattaga
    14461 aaaccagaat ttaataactt ttatagcttg gggtatgggg ttaatgttgt ttttcttgat
    14521 gaatataaca ctcattgtca ataaaacagt caaagttata aaaaggtgaa taaataacta
    14581 attatattgt attggccgtt cttcaaataa aaaataattt gaataaggtg gaagtgataa
    14641 acagtgcttt gcgaatctag agtcattaat caaaacccta aatatagaat tattaaatac
    14701 aataatgaat atttcatggt tgatttagta agtacttgga ttgcttattt tttgcccatg
    14761 attaattggt ttattcccaa aaagtacgcg aaaattagta gagaagaatt tgaaagttta
    14821 aatattgtca aacccgctaa aaataatact ttctggcctg ttgcaggatt tgcagtgtta
    14881 ttaacaacct taacaagaaa atatatctat ttgcttaaca tccatttaga aaaagaaata
    14941 gttatattaa catgctgtat gatacttcta ggtgttttcg cattgtttat atatataaat
    15001 acaaaattga agttacatat ttttgataaa aataaaagta ataacgaaaa gatcatatta
    15061 atacctacat ttaaaaatat ttgtttatcc ttatttgctt atatattatt tggtggattg
    15121 tcaacaatgg ctctgagtat gttagtaact tcatcccctc aaaatataat agaatttctt
    15181 gctttaattg gcatgactgc atgcttcttt ctactgaata tgtcatcggt tctagataaa
    15241 aaaattcatg ttattttaaa aacaaataag tagtaaaatt gattaactta ggtagtatcg
    15301 gatacttaaa tgttggttca taaaaagcaa tgattttaaa tcgaggagct atcttagaac
    15361 agggaaataa aacagccaaa gttataaaaa gtgaattaat aactaattat attatgttag
    15421 ccacgcttca aataaaaaat aattagaata aggtgggatt gataatcaat gctatgcgaa
    15481 tctaaaatca tcaataaaaa ccccaaatat agaattatta aatataatga tgagtactta
    15541 atg
//
LOCUS       DANRDI010000051        15466 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.051, whole genome shotgun sequence.
ACCESSION   DANRDI010000051 DANRDI010000000
VERSION     DANRDI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.051"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            64..651
                     /locus_tag="PZ377_001259"
     CDS             64..651
                     /locus_tag="PZ377_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDM6358138.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(794..1687)
                     /locus_tag="PZ377_001260"
     CDS             complement(794..1687)
                     /locus_tag="PZ377_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDM6358139.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            1935..2228
                     /gene="esxA"
                     /locus_tag="PZ377_001261"
     CDS             1935..2228
                     /gene="esxA"
                     /locus_tag="PZ377_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDM6358140.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            2312..5341
                     /gene="esaA"
                     /locus_tag="PZ377_001262"
     CDS             2312..5341
                     /gene="esaA"
                     /locus_tag="PZ377_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDM6358141.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            5341..5799
                     /gene="essA"
                     /locus_tag="PZ377_001263"
     CDS             5341..5799
                     /gene="essA"
                     /locus_tag="PZ377_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDM6358142.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKNSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            5771..6013
                     /gene="esaB"
                     /locus_tag="PZ377_001264"
     CDS             5771..6013
                     /gene="esaB"
                     /locus_tag="PZ377_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDM6358143.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            6026..7360
                     /gene="essB"
                     /locus_tag="PZ377_001265"
     CDS             6026..7360
                     /gene="essB"
                     /locus_tag="PZ377_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDM6358144.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            7382..11821
                     /gene="essC"
                     /locus_tag="PZ377_001266"
     CDS             7382..11821
                     /gene="essC"
                     /locus_tag="PZ377_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDM6358145.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            11851..12243
                     /gene="esaC"
                     /locus_tag="PZ377_001267"
     CDS             11851..12243
                     /gene="esaC"
                     /locus_tag="PZ377_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HDM6358146.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            12259..12573
                     /gene="esxB"
                     /locus_tag="PZ377_001268"
     CDS             12259..12573
                     /gene="esxB"
                     /locus_tag="PZ377_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HDM6358147.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            12573..13247
                     /locus_tag="PZ377_001269"
     CDS             12573..13247
                     /locus_tag="PZ377_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HDM6358148.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            13247..13564
                     /locus_tag="PZ377_001270"
     CDS             13247..13564
                     /locus_tag="PZ377_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358149.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            13574..15418
                     /locus_tag="PZ377_001271"
     CDS             13574..15418
                     /locus_tag="PZ377_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HDM6358150.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
BASE COUNT         5871 a         2149 c         2923 g         4523 t
ORIGIN      
        1 gatatcgaaa atgaaggtaa gacaatgtaa aggaaagaca taaaaaataa taggagtcta
       61 accatgataa tttttatttt aataacaata tttgctattt attatatagc tatgattgct
      121 agtttgttta aaagtgaagg tttttcaata ataggtttaa tattagatgt tgttataatg
      181 gggacattga ttttttacta ttttataggc gctcgctttg ttgatcatga tttaagcaac
      241 tttttaatgt ttatggatac tggatcatat atttttatgt actttgcaat taagtgttta
      301 tgggtgaaac ctaaggtagt aaattatttg attgcaaaag aattgggtga atctaaagag
      361 gtcattgaag agcaggaatt agatttacag acatcaaaga taagaggtat ctactttttc
      421 attatttcag tagtgatgtt aattatcact aaattaagaa tgcaacctga gttacaggca
      481 gatgctatat cgatgaatcc tgtatttatt tttgttggtg ttataatcat tttaatttgg
      541 ctagtactgg atatatatcg caagaaaaaa tacggtatat tcttattcaa gacgatagtg
      601 ccactagttg ttacgacttg gattattata gctacaatta tactttcgta aacgattttg
      661 attaatgggg aatgtagaga acatttcttt gtaattttat aatcgaataa catacaaaat
      721 aaagcgacca attttgcagt gttacgaatt gtaaactgcg aactggtcgc tttttattga
      781 tgtcatgatt aatttaatgg atgtaattat atgatgaaac ttctgaagca gagatggttc
      841 ttgatgaaac gatatattca ccaatccagt tcatttctga aattagaata cttccatcaa
      901 tattaacttt ttcaacgtag gctacatgac caaatggacc atttactgtt tgtaaaattg
      961 atcctcgtgt tgggtgtcta tctactttga agccattgct tgaagcttgg cctgcccagt
     1021 ttttagcatc tccccaaaat gtactaatcg tgtgtccatc tttggcacgt ttatcaaaga
     1081 cataccatgt acattgtcca gcagtatata agttgttctt acttgtgata agaggctgat
     1141 caatgatttt accgttacct aatgctaaag gtttaccgtc agcggtcttt gctttgtcat
     1201 taaattcggc gatttgtaat tcgtcataca attcgtctaa atcgatgcct gtaataagcc
     1261 ctttgttatc ttttgaaaaa gcgttattta attcatcgtc attgtcttcg acattcggta
     1321 ttgctggtgt caaaggattg cttggtgacg tttgaggcgg tgtgtgtgaa tcaattgcgt
     1381 cattaatgtg cgtatactga ccacttaatg aagaatggta ctgattgttg ttaagatcac
     1441 gttgatttgc gtgttgatga ttgtcgtttg tacgtgactg gttttgatga ttgttgtttg
     1501 gcgtgttgtt tttgtcatat gtataagtat acgcgccggt gtctttattc actttgaact
     1561 gtgcgtttgg gtgtgctttc tttgcttctt ctaatgtttt gctatcattc gtatatgctt
     1621 gagccgagtt aggcgacata ctaaataaag taagagttgt catcgtcagt aaaattgttt
     1681 tcttcataat aaccatttaa tcctttatgt atttaattta attttagtat acacatttat
     1741 attacaaaaa tgaatggtta attaaaaata tatgggtata ttcaatatat ttatttaaaa
     1801 aaagctaaaa atacttaaaa aactatatac atatactaat aatttatata ttatttgagt
     1861 aaggagcact ttttcaaaaa atagtgtccc taaaaagttt tgataaactt aaaatattca
     1921 ggaggtttct agttatggca atgattaaga tgagtccaga ggaaatcaga gcaaaatcgc
     1981 aatcttacgg gcaaggttca gaccaaatcc gtcaaatttt atctgattta acacgtgcac
     2041 aaggtgaaat tgcagcgaac tgggaaggtc aagctttcag ccgtttcgaa gagcaattcc
     2101 aacaacttag tcctaaagta gaaaaatttg cacaattatt agaagaaatt aaacaacaat
     2161 tgaatagcac tgctgatgcc gttcaagaac aagaccaaca actttctaat aatttcggtt
     2221 tgcaataagc attctgaaat tggcaaagtc acattttcta atgtggcttt gcttatcatt
     2281 tttttaagaa aacaactgaa aggaaataag catgaaaaag aaaaattgga tttatgcatt
     2341 aattgtcact ttaattatta taattgccat agttagtatg atattttttg ttcaaacaaa
     2401 atatggagat caatcagaaa aaggatccca aagtgtaagt aataaaaata ataaaataca
     2461 tatcgcaatt gttaacgagg atcaaccaac gacatataac ggtaaaaaag ttgagctggg
     2521 tcaagcattt attaaaaggt tagcaaatga gaaaaactat aaatttgaaa cagtaacaag
     2581 aaacgttgct gagtctggtt tgaaaaatgg tggataccaa gtcatgattg ttatcccaga
     2641 aaacttttca aaattggcaa tgcaattaga cgctaaaaca ccatcgaaaa tatcgctaca
     2701 gtataaaaca gctgtaggac aaaaagaaga agtagctaaa aacacagaaa aagttgtaag
     2761 taatgtactt aacgacttta acaaaaactt agtcgaaatt tatttaacaa gcatcattga
     2821 taatttacat aatgcacaaa aaaatgttgg cgctattatg acgcgtgaac atggtgtgaa
     2881 tagtaaattc tcgaattact tattaaatcc aattaacgac ttcccggaat tatttacaga
     2941 tacgcttgta aattcaattt ctgcaaacaa agacattaca aaatggttcc aaacatacaa
     3001 taaatcatta ttgagtgcga attcagatac gttcagagtg aacacagatt ataatgtttc
     3061 gactttaatt gaaaaacaaa attcattatt tgacgagcac aatacagcga tggataaaat
     3121 gttacaagat tataaatcgc aaaaagatag cgtggaactt gataactata tcaatgcatt
     3181 aaaacagatg gacagccaaa ttgatcaaca atcaagtatg caagatacag gtaaagaaga
     3241 atataaacaa actgttaaag aaaacttaga taaattaaga gaaatcattc aatcacaaga
     3301 gtcaccattt tcaaaaggta tgattgaaga ctatcgtaag caattaacag aatcactgca
     3361 agatgagctt gcaaataaca aagacttaca agatgcgcta aatagcatta aaatgaacaa
     3421 tgctcaattc gctgaaaact tagagaaaca acttcatgat gatattgtca aagaacctga
     3481 tacagataca acatttatct ataacatgtc taaacaagac tttatagctg caggtttaaa
     3541 tgaggatgaa gctaataaat acgaagcaat tgtcaaagaa gcaaaacgtt ataaaaatga
     3601 atataatttg aaaaaaccgt tagcagaaca cattaattta acagattacg ataaccaagt
     3661 tgcgcaagac acaagtagtt tgattaatga tggtgtcaaa gtgcaacgta ctgaaacgat
     3721 taaaagtaat gatattaatc aattaactgt tgcaacagat cctcatttta attttgaagg
     3781 cgacattaaa attaatggta aaaaatatga cattaaggat caaagtgttc aactcgatac
     3841 atctaacaag gaatataaag ttgaagtcaa tggcgttgct aaattgaaaa aggatgctga
     3901 gaaagatttc ttaaaagata aaacaatgca tttacaattg ttatttggac aagcaaatcg
     3961 tcaagatgaa ccaaatgata agaaagcaac gagtgttgtg gatgtaacat tgaatcataa
     4021 ccttgatggt cgcttatcga aagatgcatt aagccagcaa ttgagtgcat tatctaggtt
     4081 tgatgcgcat tataaaatgt acacagatac aaaaggcaga gaagataaac cattcgacaa
     4141 caaacgttta attgatatga tggttgacca agttatcaat gacatggaaa gtttcaaaga
     4201 cgataaagta gctgtgttac atcaaattga ttcaatggaa gaaaactcag acaaactgat
     4261 tgatgacatt ttaaataaca aaaagaatac aacaaaaaat aaagaagata tttccaagct
     4321 gattgatcag ttagaaaacg ttaaaaagac ttttgctgaa gagccacaag aaccaaaaat
     4381 tgataaaggc aaaaatgatg aatttaatac gatgtcttca aatttagata aagaaattag
     4441 tagaatttct gaaaagagta cgcaattgct atcagataca caagaatcaa aatcaattgc
     4501 agattctgtt agtggccaat taaatcaagt cgacaataat gtgaataagc tacatgcgac
     4561 aggtcgagca ttaggcgtaa gagctaacga tttgaatcgt caaatggcta aaaacgataa
     4621 agataatgag ttgttcgcta aagaatttaa aaaagtatta caaaattcta aagatggcga
     4681 cagacaaaac caagcattaa aagcatttat gagtaatccg gttcaaaaga aaaacttaga
     4741 aaatgtttta gctaataatg gtaatacaga cgtgatttca ccgacattat tcgtattatt
     4801 gatgtattta ctatcaatga ttacagcata tattttctat agttatgaac gtgccaaagg
     4861 acaaatgaat ttcattaaag atgattatag tagtaaaaac catctttgga ataatgtcat
     4921 tacgtcaggt gttattggta caactggttt ggtagaaggg ttaattgtcg gtttaattgc
     4981 aatgaataag ttccatgtat tagctggcta tagagcgaaa ttcatcttaa tggtgatttt
     5041 aactatgatg gtcttcgtac ttattaatac gtatttacta agacaggtaa aatctatcgg
     5101 tatgttctta atgattgctg cattgggtct atactttgta gctatgaata atttgaaagc
     5161 agctggacaa ggtgtgacta ataaaatttc accattgtct tatatcgata acatgttctt
     5221 caattattta aatgcagagc atcctatagg cttggtgcta gtaatattaa cagtacttgt
     5281 gattattggc tttgtactga acatgtttat aaaacacttt aagaaagaga gattaatcta
     5341 atgttgatga atagcgtgat tgctttaact tttttaacag catctagcaa taatggcgga
     5401 cttaatattg atgtgcaaca agaagaggaa aagcgaatca ataatgattt aaatcaatat
     5461 gatacaacgc tatttaataa aaacagcaaa gcggttaatg atgcgattgc taagcagaaa
     5521 aaagaacgac aacaacaaat aaaaaatgat atgtttcaaa atcaagcgag tcactcgact
     5581 cgcttgaatg aaactaaaaa agtgttattt tccaaatcta acttagaaaa gacttcggag
     5641 agtgataaaa gcccctatat tcaaaacaag caggagaaaa aaatattccc gtacattttg
     5701 atgtctgtag gggctttttt gactttagga tttgtcattt tttcaattca taaagggaga
     5761 cgaacgaaaa atgaatcagc acgtaaaagt aacatttgat tttactaatt ataattacgg
     5821 cacatatgac ttagcagtac cagcatattt accgataaaa aacttaatag ctttagtatt
     5881 ggatagtttg gacatttcaa tatttgatgt caatacacaa attaaagtga tgacgaaagg
     5941 tcaattactt gttgaaaatg atcgactcat tgattatcaa atcgctgatg gagatatttt
     6001 gaagttacta taggaggaaa aatagatggt taaaaatcat aaccctaaaa atgaaatgca
     6061 agatatgtta acgcctttag atgctgaaga agcagctaaa acaaaattac gcttagatat
     6121 gagagagatt cctaagtctt caattaaacc agaacatttt catttaatgt acttattaga
     6181 acaacattct ccatatttta tagatgctga attaactgaa ctacgtgaca gtttccaaat
     6241 acattatgac attaatgaca atcatacacc ttttgataat attaaatcat ttactaaaaa
     6301 tgaaaaatta cgttacttac tcaatatcaa aaatttagaa gaagtaaatc gtacacgcta
     6361 cacatttgtg ttggcaccag atgaattatt tttcacaaga gatggattac ccattgctaa
     6421 aacaagaggg ttacaaaatg ttgttgatcc attacctgtg tcagaagctg aatttttaac
     6481 aagatataaa gcgctggtta tctgtgcatt caatgagaaa caatcatttg atgctttagt
     6541 tgaaggaaac ttagaactac ataaaggaac gccatttgaa actaaagtta ttgaagcggc
     6601 aacgttagat ttactaacgg catttttaga tgaacagtat cagaaacaag aacaagatta
     6661 tagtcaaaat tatgcatatg tacgcaaagt aggacatacc gttttcaaat gggttgctat
     6721 cggtatgaca acgttaagtg ttttattaat tgcattctta gcctttttat atttttcagt
     6781 aatgaagcat aatgagcgca ttgaaaaagg ataccaagca tttgtaaagg atgattatac
     6841 gcaagtacta aatacgtatg atgatttaga tggtaaaaaa ttagataaag aggcacttta
     6901 catttatgcc aaaagttata tccaaacaaa taaacaaggt ttagaaaaag ataagaaaga
     6961 aaatttactt aataatgtga caccaaattc aaacaaagac tacttattat attggatgga
     7021 attaggacaa ggacatcttg atgaagcgat taatattgcc acttatttag atgataacga
     7081 tattacaaag ttagcgttga ttaataaatt aaatgagatt aaaaataacg gagatttatc
     7141 gaatgataaa cgttctgaag aaacgaaaaa gtataacgat aaattgcaag atattttaga
     7201 caaagaaaaa caagttaaag atgaaaaagc gaaatctgaa gaagagaaag caaaagcgaa
     7261 agatgagaaa ttaaagcaac aagaagagaa cgaaaagaaa caaaaagaac aagcacaaaa
     7321 agataaagaa aaacgccaag aagctgaaag aaaaaaatag tataggactg aggcaaagac
     7381 aatgcataaa ttgattataa aatataacaa acaattgaag atgctcaatt tgcgagatgg
     7441 taagacatat actattagcg aagacgagcg tgcagatatt acgttgaaat cgttaggcga
     7501 agtcattcat ttagaacaaa ataatcaagg tacttggcaa gcgaatcata cttctattaa
     7561 taaggtgctt gttagaaaag gtgaccttga tgacattaca ttacagcttt atacagaagc
     7621 tgattatgca tcatttgcgt atccttcaat tcaagatacg atgacaattg gaccaaatgc
     7681 gtatgatgat atggttattc aaagcttgat gaatgccatc attattaaag attttcaatc
     7741 aatacaagaa tcacaatacg tacgcattgt gcacgataaa aatacagatg tgtatattaa
     7801 ctatgaacta caagagcaac taacgaacaa agcttacatt ggtgatcata tttatgttga
     7861 agggatatgg ctcgaagtac aagctgatgg tttaaatgta ttgagtcaga atacagtggc
     7921 atcgtcatta attcgcttaa cacaagagat gccacatgca caggcagatg attacaatac
     7981 gtaccatcgt tcgccaagga ttattcaccg tgaaccgacg gatgatatta agattgaaag
     8041 accgccacag ccaatacaga agaacaatac agtgatatgg cgttccatta taccgccatt
     8101 agtaatgatt gctttaactg ttgtcatctt tttagtgaga ccaattggta tttatatttt
     8161 aatgatgatt ggtatgagta cagtaacgat agtatttggt attacaacgt atttctctga
     8221 aaagaaaaag tataacaaag atgttgaaaa acgagagaaa gattacaaag cttatttgga
     8281 taataaatct aaagaaatta acaaagcgat taaagcacaa cgttttagtt tgaattacca
     8341 ttatccaacg gttgctgaaa ttaaagatat cgttgaaacg aaagcaccaa gaatatatga
     8401 aaaaacatcg catcatcacg atttcttaca ttataagtta ggtattgcga atgtagaaaa
     8461 gtcattcaaa ttagattacc aagaagaaga atttaaccaa cgtcgtgatg aactattcga
     8521 cgatgctaaa gaattgtatg aattttacac agatgtagaa caagcaccat taatcaatga
     8581 tttaaatcat gggccaattg catatattgg tgcacgacat ctcattttag aagaattgga
     8641 gaaaatgcta attcaattgt caacattcca tagttatcat gatttagagt ttctatttgt
     8701 gacacgtgaa gatgaagttg aaacattgaa atgggcacgt tggttgccac atatgacatt
     8761 gagagggcaa aacattagag gatttgttta caatcaacga acgcgtgacc aaattttaac
     8821 gtcaatttat agcatgatta aagaacgtat ccaagctgtg cgtgaacgca gcagaagtaa
     8881 tgagcaaatt attttcacac cgcaattagt gtttgtcatt acagatatgt cattaattat
     8941 tgatcatgtc attttagaat atgtaaacca agatttatca gaatatggta tttcattaat
     9001 ctttgttgaa gatgtgattg aaagtttgcc agagcatgta gataccatta ttgatatcaa
     9061 gtctcgtact gaaggcgaac tgattacgaa agaaaaagaa ttagttcaat tgaaatttac
     9121 acctgaaaat attgataacg tcgataaaga atatatcgcg cgacgtttgg cgaatttgat
     9181 acacgtcgaa catttgaaaa atgcaattcc tgatagtatt acatttttag agatgtataa
     9241 cgtgaaagaa gtagatcagc ttgatgtggt taatcgatgg agacaaaacg aaacatacaa
     9301 aacgatggca gtacctttag gtgtaagagg taaagatgat attttatcat tgaacttaca
     9361 tgaaaaagca cacgggccac atggtttagt tgctggtacc actggttcag ggaaatctga
     9421 gattatccaa tcatacattt tatctttagc tattaatttt caccctcatg aagttgcatt
     9481 cctattgatt gactataaag gtgggggtat ggcgaactta tttaaagatt tagtccattt
     9541 agttggtacg attacaaact tagatggcga tgaagcgatg cgtgccttaa catcaatcaa
     9601 agccgaattg agaaaacgtc aacgtttatt cggagagcat gatgttaacc atattaatca
     9661 ataccataag ttatttaaag aaggtattgc gacagaacca atgccacatt tattcattat
     9721 ttccgatgag tttgccgaat taaaatcaga acaacctgat tttatgaaag aacttgtatc
     9781 aacggcacgt attggacgtt cgttaggtat tcatttaata cttgcgacac aaaaaccatc
     9841 gggtgttgtt gatgaccaaa tttggtctaa ctctaaattt aagttggcat taaaagtaca
     9901 agatagacaa gacagtaatg aaattttaaa aacaccagat gcagcagaca ttacattacc
     9961 aggtcgtgcg tatttacaag ttggtaataa tgaaatttat gaattattcc aatctgcatg
    10021 gagtggtgca acatatgaca tcgaaggcga taaattagaa gttgaagata agacgattta
    10081 catgattaat gactatggtc aacttcaagc aatcaacaaa gacttgagtg gacttgaaga
    10141 tgaagaaacg aaagaaaatc aaactgagtt agaagcggtc atagatcata tcgaatctat
    10201 tacaacacga ttagaaatcg aagaagttaa gcgtccatgg ctaccaccat tgccagaaaa
    10261 tgtatatcaa gaagatttag tagaaacaga tttcagaaaa ttatggtcag atgatgcaaa
    10321 agaagtggaa ttaacattag gacttaaaga cgtaccagaa gaacaatatc aaggaccgat
    10381 ggtattgcaa ttgaaaaaag ctgggcacat cgcgttaatc ggaagtccag gatatggtag
    10441 aacaacgttc ttacacaaca ttattttcga tgttgcaaga caccatcgtc ctgatcaagc
    10501 acacatgtac ttgttcgatt tcggtaccaa tggtttgatg ccagttacag acataccaca
    10561 tgtcgctgat tactttacag tagatcaaga agacaagatt gctaaggcga tacgtatatt
    10621 taatgatgaa attgatcgtc gtaagaagat tttaagtcag tatcgtgtca ctagtatttc
    10681 tgaatatcga aaattaactg gtgaaacaat tccgcatgtc tttattctta ttgataactt
    10741 tgacgcagta aaagattcac ctttccaaga agtttttgaa aatatgatga ttaaaatgac
    10801 gcgtgaaggg ctagcattag acatgcaagt aaccttaact gcttcaagag ctaacgctat
    10861 gaaaacacca atgtacatta atatgaaaac gcgtatcgca atgtttttat atgataaatc
    10921 agaggtgtcg aacgtagtag gacagcaaaa atttgcggtt aaagatgttg tgggtcgagc
    10981 attgttaagt agtgatgaca acgtatcatt ccatattggc caaccattta aacatgatga
    11041 gaccaaatca tataatgatc aaattaatga tgaagtatcg gcgatgacag aattttataa
    11101 aggtgaaaca ccaaatgata ttcctatgat gccagatgaa attaaatatg aagattacag
    11161 agaatcatta aacttaccag atatagttgc aaatggtgct ttaccaattg gattagatta
    11221 tgaaggtgtt acactacaaa aaattaaatt aactgaacca gcaatgattt catcagaaaa
    11281 tccgagagaa attgcgcata ttgctgaaat tatgatgaaa gaaattgaca tattaaatga
    11341 aaaatatgcg atttgtatcg cagactcaag tggagagttt aaagcttata ggcatcaagt
    11401 ggctaacttt gccgaagaaa gagaagacat taaagcgatt catcaactaa tgattgaaga
    11461 cttaaagcaa agagaaatgg acggcccatt tgaaaaagat tcactttata ttatcaatga
    11521 ttttaaaaca tttattgatt gcacgtatat tccggaagat gatgttaaaa agcttattac
    11581 aaaaggacca gaacttggct tgaacatttt atttgtcggc attcataaag aattaataga
    11641 tgcttatgat aaacagattg atgttgcacg taaaatgatt aaccaattta gtataggtat
    11701 tcgtatttca gaccaacaat tctttaaatt tagatttatt caacgagaac ctgttattaa
    11761 agaaaatgaa gcatatatgg tcgcaaacca agcttatcaa aagattagat ggtttaaata
    11821 gcaatgaatt aaataggagg gaggtatgtt atgaatttta atgatattga aacaatggtt
    11881 aagtcgaaat ttaaagatat taaaaagcat gctgaagaga ttgcgcatga aattgaagtt
    11941 cgttctggat atttaagaaa agctgaacaa tataagcgat tagaatttaa tttgagtttt
    12001 gcactagatg atattgaaag cacagcaaag gacgtacaaa ctgcaaaatc tagtgctaat
    12061 aaggacagtg taactgttaa gggaaaggcg cccaatacgt tatatattga aaaaagaaat
    12121 ttgatgaaac aaaagcttga aatgttgggt gaagatatcg ataaaaataa agaatccctc
    12181 caaaaagcta aggaaattgc tggcgaaaag gcaagtgaat attttaataa agcaatgaat
    12241 taatattgag gtgaagatat gggtggatat aaaggtatta aagcagatgg tggcaaggtt
    12301 gatcaagcga aacaattagc ggcaaaaaca gctaaagata ttgaagcatg tcaaaagcaa
    12361 acgcaacagc tcgctgagta tatcgaaggt agtgattggg aaggacagtt cgccaataag
    12421 gtgaaagatg tgttactcat tatggcaaag tttcaagaag aattagtaca accgatggct
    12481 gaccatcaaa aagcaattga taacttaagt caaaatctag cgaaatacga tacattatca
    12541 attaagcaag ggcttgatag ggtgaaccca tgatgaaaga tgttaagcga atagattatt
    12601 tttcttacga agaattaaca attttaggtg gtagtaaatt gcctctcgta aattttgaat
    12661 tgtttgatcc atcaaatttt gaagaagcta aagctgcttt aattgaaaag gaattagtaa
    12721 cagagaatga caagttaact gatgcaggtt ttaaagtggc tacattagtc agagagtata
    12781 ttagcgccat tgtaaatatt cgaattaatg atatgtattt tgcaccattt agctatgaaa
    12841 aagatgaata tattttgtta agccggttta aaaataatgg gtttcaaata cgaattatca
    12901 ataaagacat tgcatggtgg tcgattgtac aatcatatcc tttattgatg agacaagaaa
    12961 agtccaatga ttgggacttt aaacaaattg acgatgaaac attggagaac ttaaataatg
    13021 aaagtatcga tacgattggg cgtgttttag aaattgaaat atacaatcat caaggtgacc
    13081 ctcaacaaag tttatataac atttatgaac aaaatgattt gttattcatt cgatacccat
    13141 taaaagataa agtgctgaat gttcatattg gtgtcattaa tacatttata cgagaattat
    13201 ttggattcga tactgatgaa aatcatatta ataaagcaga ggagtaatga cgttgagtgg
    13261 aaaaattagt gttaaagctg aaacgattgc acatgttgta aaagaattgg aaagcataag
    13321 tcaaaagtat gatgaaatag ctcaaaactt tggaaaaata gcgcaattaa attactacag
    13381 tagtgaaaaa gctgcacatt ctatggaaaa tggctatagt agtgctgcaa cagtcattag
    13441 tggtctcaaa ggtccattga gtacactcgg tggtggcgtc atgaattcag cacaaaagtt
    13501 ctttgaagca gatgaacatt ggggtacgga atttgccaag ctttactata atattgaggg
    13561 ataggtgcat gacatgacaa aagatattga atatctaaca gctgattatg acaatgaaaa
    13621 gtcatctatc caaagtgtaa tagatgcaat agagggtcaa gacttcttag atgtagatac
    13681 aacaatggat gatgcggtaa gcgatgtcag ttctttagac gaagatggcg caatatcatt
    13741 aacaagtagt gtagtaggtc cacaaggatc taaattaatg gggtattatc aaaatgagtt
    13801 atatgattat gcatctcaat tagattcgaa aatgaaagaa attattgaca cgccatttat
    13861 agaagatata gataaagcat tcaaaggtat aacgaatgtt aaattggaaa atatactaat
    13921 taaaaatggt ggtggacatg gtagagatac ctatggggct tctgggaaaa ttgcaaaggg
    13981 agatgccaag aaaagtgaca gcgatgttta tagcatcgat gaaatattaa aatcggatca
    14041 agaatttgta aaagtaattg atcagcatta caaagaaatg aaaaaagaag ataagaaatt
    14101 atctaaaagt gattttgaaa aaatgatgac tcagggcgct tcttgtgatt acatgacagt
    14161 agctgaagcg gaagagctag aggagcaaaa gaaaaaagaa gaagctatag agattgcagc
    14221 actagctggt atggtagttt tatcttgtat taatcctgtt gctggagcag tagctattgg
    14281 tgcttattcc gcttattcag cagcaaatgc agccacagga aaaaatattg taactggaag
    14341 aaagctatct aaagaagaac gaatcatgga aggactttcg cttattccat tgccaggtat
    14401 gggcttcctc aaaggtgctg ggaaaagttt aatgaaatta ggcttcaaag gcggagaaaa
    14461 atttgcagtt aaaacaggat tgcaaaagac aatgcaacaa gcagttagtc gtatttcacc
    14521 taaaatggga atgatgaaaa acagtgtgtt gaatcaatct cgtaactttg ctcaaaatac
    14581 tcatgttgga caaatgctga gtaacatgcg tggtcaagca actcatactg ttcaacaaag
    14641 tagaaattgg attggacaac aagcacaaaa tgtcaaacga atagtgaata atggacttga
    14701 taaagaaata gcacatccat ttaaacaaca acttgcacca gcgggaatgg gtggtataaa
    14761 atttgctgaa acaactactt tgagaaacat gggtcaaaac ataaagcgtg ctgttacacc
    14821 acaaaatcac gtgacacatg gtccaaaaga tagtatggtg agaagtgaag gcaaacatag
    14881 tataagtagc catgaaatga attcatcaaa gtatgttgaa tcaccaaact acaccaaggt
    14941 tgaattcgga gaacactatg caagacttag acctaagaaa ctaaaggcga atattgaata
    15001 cacaacacct actggtcaca tatatcgaac cgatcataaa ggtcgcataa aagaagttta
    15061 tgtagacaat ctctctctaa aagatgggga tcgtaatagc catgcacaaa gaactgtggg
    15121 gggagaggat agattaccag acgatgatgg aggtcactta atcgctagaa tgtttggtgg
    15181 ttcaaaagac attgataacc ttgtggcaca aagtaaattt atcaaccgtc catttaagga
    15241 gaaaggtcat tggtataatc ttgagaaaga gtggcaagag ttcttaaact ctgggaaaga
    15301 ggtgaaaaat attaaaatgg aagtaaaata tagcggtaat agtcaaagac cgactatatt
    15361 taaagttgaa tatgaaatta atggtgaaag aaatattaga agaatattaa ataagtagag
    15421 gtgccaacat gacatttgaa gagaagctta gcaaaatata caatga
//
LOCUS       DANRDI010000052        15299 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.052, whole genome shotgun sequence.
ACCESSION   DANRDI010000052 DANRDI010000000
VERSION     DANRDI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.052"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..492)
                     /locus_tag="PZ377_001272"
     CDS             complement(<1..492)
                     /locus_tag="PZ377_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDM6358151.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPY"
     gene            878..1128
                     /locus_tag="PZ377_001273"
                     /pseudo
     CDS             878..1128
                     /locus_tag="PZ377_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            1450..1629
                     /locus_tag="PZ377_001274"
     CDS             1450..1629
                     /locus_tag="PZ377_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358152.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            1940..2200
                     /locus_tag="PZ377_001275"
     CDS             1940..2200
                     /locus_tag="PZ377_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358153.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(2253..2603)
                     /gene="scn"
                     /locus_tag="PZ377_001276"
     CDS             complement(2253..2603)
                     /gene="scn"
                     /locus_tag="PZ377_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HDM6358154.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
     gene            3288..3737
                     /locus_tag="PZ377_001277"
     CDS             3288..3737
                     /locus_tag="PZ377_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HDM6358155.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            complement(3832..4167)
                     /locus_tag="PZ377_001278"
                     /pseudo
     CDS             complement(3832..4167)
                     /locus_tag="PZ377_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(4817..5308)
                     /gene="sak"
                     /locus_tag="PZ377_001279"
     CDS             complement(4817..5308)
                     /gene="sak"
                     /locus_tag="PZ377_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HDM6358156.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            complement(5499..6254)
                     /locus_tag="PZ377_001280"
     CDS             complement(5499..6254)
                     /locus_tag="PZ377_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDM6358157.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            complement(6266..6520)
                     /locus_tag="PZ377_001281"
     CDS             complement(6266..6520)
                     /locus_tag="PZ377_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDM6358158.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            6572..6679
                     /locus_tag="PZ377_001282"
     CDS             6572..6679
                     /locus_tag="PZ377_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358159.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            complement(6732..6908)
                     /locus_tag="PZ377_001283"
     CDS             complement(6732..6908)
                     /locus_tag="PZ377_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDM6358160.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(7058..7354)
                     /locus_tag="PZ377_001284"
     CDS             complement(7058..7354)
                     /locus_tag="PZ377_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDM6358161.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            complement(7412..7699)
                     /locus_tag="PZ377_001285"
     CDS             complement(7412..7699)
                     /locus_tag="PZ377_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358162.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            complement(7746..7898)
                     /locus_tag="PZ377_001286"
     CDS             complement(7746..7898)
                     /locus_tag="PZ377_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358163.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            complement(7888..11673)
                     /locus_tag="PZ377_001287"
     CDS             complement(7888..11673)
                     /locus_tag="PZ377_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358164.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            complement(11689..13173)
                     /locus_tag="PZ377_001288"
     CDS             complement(11689..13173)
                     /locus_tag="PZ377_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDM6358165.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            complement(13170..>15299)
                     /locus_tag="PZ377_001289"
     CDS             complement(13170..>15299)
                     /locus_tag="PZ377_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDM6358166.1"
                     /translation="TNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIF
                     SNLRNWMANIWNSIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAI
                     KKGLNKLIEGLNWVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGR
                     GNGPNGFRNEIIEFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGT
                     MWKDIKSGASSAFNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNS
                     LTKGMGIAGDITKAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAA
                     YTAATGRPFHEGVDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHL
                     KNFSKSPPSGTMVKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGR
                     LSIGGGGATSGSGATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNN
                     WDINAQRGDPSRGLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKR
                     AGDYAYATGGKVFDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKN
                     KRPSQLSSVNGFDDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDK
                     KVNASIEKRERQESTKVKFRKGGIAIQ"
BASE COUNT         4730 a         2861 c         2099 g         5609 t
ORIGIN      
        1 gtatggaact tggtaatttg ctttatcttt ctttttatct tcaacttgct ttttagtatc
       61 tacgttaata ttaatagctt taatttcact tgtattaatt aaatcagctg tgtaaatacc
      121 tgctttcaaa tcgataactt tctttgttcc atttttaaag taaacagtat atttcgcttg
      181 cttcgatagt cttaaatcta tatcactaat acctctgtct gattttaaaa ctgatttaac
      241 tctatcctct aaatctttat aactaatatt ttgattctta ttaaatgtta agcttgataa
      301 aatattttgg cttgtaccgt tcacagtgat tgcatatgga acatggactt tagaatatcc
      361 atggtgtaac gaacttgatg atttatctaa tggcttagct gcggcagacg cttcattatt
      421 attaaagttt gcacctgttg atgctaaaac acctaatgct aaagttgttg taatcaatga
      481 cttaaatttc ataaattatc tctccttttt tgtgtaattc gtatttgcaa cttaattata
      541 gccagacttt ctctattttt tgaattaact gaatattaat aataaattat ctttaacaat
      601 aattttttaa cactgttaaa agttctttta attttgatta actaattaat ttacaatacc
      661 taaaatgttg tttggttttg tttataccaa gcttcaaact taaatgtcat aacaacattc
      721 atttcttaat tccgattaga tttgtcgatt atatttacag catctttata ctcaaaaaac
      781 atttacttaa aaatataaat tcgatttaat aattaattta aatttagtta atcaattttg
      841 catctatttt gttgtaagct atataaaagg agtgataatg atggtgaaaa aaacaaaatc
      901 caattcacta aaaaaagttg caacacttgc attagcaaat ttattattag ttggtgcact
      961 tactgacaat agtgccaaag ccgaatctaa gaaagatgat actgatttga agttagttag
     1021 tcataacgtt tatatgttat cgaccgtttt gtatccaaac tggagacttt taacataaaa
     1081 ttacttatca ttcaaaaagt aaaacagcat aatatcaagg tttataactt tatcattatc
     1141 aataatacct catataaaat aaaattttag ggacttttta gggactttaa atttaaaatt
     1201 acaagtttaa tagaaacatc aaaataatca catgtttgtg tggaatgtac accccaaaag
     1261 ctagactgaa aaatctattt tttgaggtgt atttttatag gtaaatataa taaattagag
     1321 tagacaactc agaattccaa ttttataata atattgcttg acatatcaaa ctagatagta
     1381 ctattttgaa tatattatta taatcaaaaa ttcattgtaa acttttagac aaaaggaagt
     1441 aataaaaacg tgaaatttaa aacaactaaa gaatgtaaaa gtaataatat ctttaaaaga
     1501 agtcaagaaa ttaataatag agaaagtgaa aagggttgtt tatggggcat tagcatgttg
     1561 attctacttt tcttattgat tctgtttgga ataactgctt gttcatcaag cattcacttt
     1621 attaattaga tttttttact tggaggtatc atgtgaagaa ccatacaaat ataattaata
     1681 tcttattagt tatagtcaac tcattaactc attttctaac tctaaacacc tcatttttta
     1741 ataattcagc atcggatttc tgttttatca taggggctat atttttctta atcggaattt
     1801 ttgttgcaat atacggtatg aagcgagcaa catattggtt aaacttattg attttattta
     1861 ccaatatttt ttattttcta cacttctgtg tgttactttt gttaaaatat ataggattta
     1921 aattatttat ttatgaaggg tgtgtattgt tatttatcta atttatagtc taattgtcta
     1981 tttcatctgc attataaatt ttttcataat ggctgcacca ttcttaatga ctaatgctga
     2041 ttatgtatgg acgcctatga ctacagttac gttatttatt ttgagtttga ttattttctt
     2101 gatatttata aaaacaaaag atgtcgttca tttaacaatt ttcatattaa acttactttt
     2161 ttcaatactt tattgtttgc ctatactgtt ttatttatga acacttttat attatctaga
     2221 aaattaaaac cacccgtaaa agggtggttt ttttaatatt tactttttag tgcttcgtca
     2281 atttcgttat aaatcttttg aagttgatat tttgcttctg acattttctt aaagtctttt
     2341 gacttaagag catacattgc tttttgacct gaaattttta tagttcgctt ataataagtg
     2401 tttaaacttc cagtagctaa ttcattaaca tttagttcat ctaataacga ttttaattca
     2461 ttagctaact tttcgttttg atattcattc gatgttggca agcttgtgct agcttgtgcc
     2521 tcatttttat ctagattagt tactagtggt gatgctaaaa cgattgctaa agttcccgca
     2581 agtatagatt ttctaatttt cattttttat tctccttagt aattagtact aatttatttt
     2641 aatgactgac taaaaaaaag tacattaatt attcattaac tatatagatc ttcttattaa
     2701 atgaaaatta ataaattatc aaattgacat accttattgt atttatagaa aaacaaaaaa
     2761 aggtaagcac cgaaatgctt acctacttcc cataaacaat ataacacata tacattgatt
     2821 tggaaagcgc aaaaataaat ttaatatact agcccgaagg ggagcaatac ataaaaaatg
     2881 aaaggcgctc cttgaaaacg cccaaggtaa tattaacata aaatggctac tattgcatta
     2941 tctaatttta tttaattaaa acaaatatat atagcacaaa aaaactagcc cgaaggctag
     3001 cttatgaata gatgaaaatt tgaacacatt gctgtgtcta agataaatat agcacatatt
     3061 aattactatt aatagaaaaa ttatagcgtt ttttagatta tttcaattta tcgagaccaa
     3121 attcatcaaa acactaattg aattttaatt tttagttaaa aataattaat cttttattaa
     3181 tttcaagtta attaatatat ttcgtcgatt agattaaact cattttagga atttcgcaaa
     3241 actaactata aatttaaata tagaatttaa ggagaattaa catcattatg aaaaagaaat
     3301 tagcaacaac agttttagca ttaagttttt taacggcagg aatcagtaca caccatcatt
     3361 cagcgaaagc ttttactttt gaaccgtttc ctacaaatga agaaatagaa tcaaataaga
     3421 aaatgttaga gaaagaaaaa gcttataaag aatcatttaa aaatagtggt cttcctacaa
     3481 cgctaggaaa attagatgaa cgtttgagaa attatttaaa gaaaggcaca aaaaattctg
     3541 ctcaatttga aaaaatggtt attttaactg aaaataaagg ttactataca gtatatctga
     3601 atacaccact tgctgaagat agaaaaaatg ttgagttact aggtaaaatg tataaaacat
     3661 acttctttaa aaaaggagag tctaaatcat cttatgtaat taatggtcct ggaaaaacta
     3721 atgaatatgc atactaatag tagttacata aattaaaagg tagatatttc ttttttatat
     3781 aaaggtttgg cagacatttc ataacttgcc aaacctttat atatctaatt atcaaactgc
     3841 actaaactta ccaaaactgc ttattctatt acctgccttg tctacctctc ctgtcgctat
     3901 ataacgacgt tgtccactat tagcaatata agtaatccat ctatagccat taatgcaata
     3961 tgcgccgtca tatttgatag ttgcgttatt gggtaataca cctgtaattc ttgaattagt
     4021 tgaatagcca tccctcacgt tattaccttt aacattggca actgtgtaat aaccagtctc
     4081 ttttttatac ggtacattgt ttttatcgag tgtataacct gctggcactg gtgaattctt
     4141 ttcattttta gctggtgttt taacattact gatacctgat acacacttcc aataaaaata
     4201 accacaccat ttaagatgcg gtgtagcgac tgtaatattt ctatgttgtt gagatatatg
     4261 tatcgagtga agggcaaaaa ggatatcaat tgcaggataa gtgttaggtt actaggccac
     4321 ttaacaggct atatagttca ctcctactat atacagttaa ttataacata aaaagcaccc
     4381 cgtaaactat tatacgggaa tgctaaagtc atatatacta cgagggagta gtatgaaaac
     4441 tatgctctct atcataagaa aaaacaccca gtgacatgct tgggtgaaca aggatagatg
     4501 taaatagttg atgcatgtgt aacacatcat aacaaaaaac tagcccgaaa gctagctata
     4561 acatacaatc taaaaagacg tcccttgaat acgtctagaa agattataac ataaaaaaat
     4621 aggcaagtac cgaagtacct gcctaaaaaa ggattatcca ctttttcatc ctaactgatt
     4681 tctccccata agtcacctaa tatctgatta ggtggggaag aaccattcgt gcatgaatga
     4741 gaatttgatg aaagataatt ttcactacac atattcaatc aagacattgc tttctataat
     4801 aaacaactat tttgttttat ttcttttcta taataacctt tgtaattaag ttgaatccag
     4861 ggtttttaat atgctctgat aaatctggga caacaaaacc tttttctgtt atagggaaag
     4921 acttcgtttc ttcttttttc ttattcttat cataataagt gacttcgatc tttgcgcttg
     4981 gatctaattc aactactcta aactctttat atgctgtcgc atctaatgcc cattcgacat
     5041 agtattcaat tttttctttt gtaagtgtag tcccaggttt aataggaaac tcgacataat
     5101 gaggggatag caattcattt cctttaccat caactccagt cacatttacc atcaaatacg
     5161 ggcctgttgg ttcaaaataa ctcgcgtcat cgcctttttt atattttcct ttgtcgaatg
     5221 aacttgatgc acttacctca ttagtaattg aagaaaatga gaataataac aataaaacag
     5281 ttaaaaataa taaacctctt ttgagcatgg cgcttcctcc aaatataata tatttgtttt
     5341 tatttatctc tttttatagc taacaccctt atttaagatg ataaatatca acaacttcat
     5401 tttatattga gaaaatatta aaaatcaata aaatattaaa agaaactaaa attacattaa
     5461 aaaataatta acaaatattt aacattttaa cctaagaatt aaaacacttc tttcacaatc
     5521 aatctctcat gccatatcca ctcattatga ttgttccaat aaatgcgaca ccaaccatct
     5581 ataatttcaa acacatatat taatgttcca ggcgcgtata cagcctgtcc aacatcgaat
     5641 ctatagttag tacgattatc accgtatcta gtggctgaag tagcacctaa gccgtcgatt
     5701 ttcgcattaa aataagcacc ttttgaccat ttaaggttat aaggcgcttt acttccaact
     5761 gttattttac ttgcagattt accgactgct ttttgagcag gtggtttaac tttatttgtg
     5821 atcttattca ttaagccctc acttttatac ttaggtctaa taaagtgagt acagccgtaa
     5881 taattatccc aacgtaactt tgcaggcgta tttgcgttac cgtcatagtt ctgttccaaa
     5941 attaaaaatt ggtttgtatt accaccatta aacactaaac caatatgacc gtattgttta
     6001 tatattcctt tggtaaatac agccacatca cctatttgtg gaacaaacga tggtgtgttt
     6061 tcatatactg ttgccatgtt tttaaaatcg ttattgattg catcttttgc atttccccac
     6121 attctaattt ctaacaacca ataaatgtaa tcaactgcta aatctgcaca ttggtaacca
     6181 taccaaccgt caaaatcaat atatctacct tgataccaac gtaaccttgc tcttgcttca
     6241 ctgtatgttt tcattatttt acctcctagt attttcttct tggttcttca tattctaaag
     6301 cttggtggct atcacctata cctttagtag tcgggtcttg aatcacacca gttaatacta
     6361 aaaatcctaa tatagcgttt aaaccgtctg ttaattgctc tgtataaact tggatatcat
     6421 acccaatagc ttttgcgatg ttttgagcaa ataaaaagat agctgacaat atcgctaccc
     6481 aaaatgattt ttgtttcatt ctaattttcc aattaatcat attcttatct ccttttaccc
     6541 aaaataaaaa gacgactaat aagccgtcta tttgatattt atattatggt gtgttaattt
     6601 atatatagaa aaagggcaac atgcgcaaac atgttaccct aatgagcccg ttaaaaagac
     6661 ggtggctatt ttagattaaa gattaaatta ataaccattt aaccatcgaa accagccaaa
     6721 gttagcgatg gttatttttt attgcttaat tcaataagct tgattactag acctatcaat
     6781 gcaataagga ataaaccaaa ctgcaacatg gtactaattg taatcattag gcgtctcctt
     6841 tctaaagatt tcagtaatgc caccataggc accacctcct tatactcaga tagccaccat
     6901 ctatccaact tgctcacttc tgcatattac cataattaca acaataaata aaaagtcagt
     6961 accgaagcac tgactaaaac ttatttacat ttaccgaacc aaaaacatgt ccagaaacta
     7021 taaccaaaga ttagtttaaa cattttattc acctctctta tatgcccata agcatacgca
     7081 ataatgctat aattagcgat ccaaatattg tcccaactaa accaagcacc cacattttca
     7141 tatcacgtat gttcttatca ttttctttct tattcttttc atctatttct ctttcttttt
     7201 gaatagcatc taaggtttta tctaatttaa tgttaacttg ctcttgggtt ttttgaccta
     7261 atttaatttc attgagtgtg ctgagcattg ttttatcatt ctcttctaac cttctgatgc
     7321 gccattcatg ttcgtgtttt ttgaaccacc ccaattcagt acacccgctt tctaaaagaa
     7381 taaagattat gagtatctaa ctcatagctt ttcatactgt ttcagtgtta actgttacct
     7441 ctggagataa atctgatctt tcaactactt ctttaactac tttcacacgt tgttttttgt
     7501 tagttaattg atataacaaa tttaacgtct ccgcaatttt cttagcgttt tcttcagatt
     7561 taaaatcttg agcatggtta accatttcag aagttgtaaa acttcctgtg aaatcttgat
     7621 atactacacg ttctgtacct tctttgtcga tttgtactaa aataaacctt tctgtattgt
     7681 tgataatttc ttttgccata attaaatgac ctccttaaat ttttgtataa aaatagtgct
     7741 aaagattact cttcctcagc acattgttga ttttctttat tttcttgtat atacgctttt
     7801 aacatcgcgt tttcttgtgt taacctcata atttcctgtg ataaataatg aattgtatat
     7861 tcaggattag cttgtaatcc ttgtttgtta tcctgcattc tttgactcct ccaatttctt
     7921 gattcttagt tgttgttctt tgataacagg gataagatga atccatagac gatcatacgc
     7981 tataccttca atttctcctt tgtcatcata cgtgacaaac tcttttaatc ctaaattctc
     8041 cacctcttca gcaatcaaac ctacgtatct atcaagttta taggtgtctt ccgataattt
     8101 tctatcttct ctcagctctc tagctaaaat ttcagactca gctttatcaa accacgttct
     8161 aataggtaag ttaagaatag cttttgaatg ttccagttgt tcatctctat cgttatattg
     8221 attttcgata gataacttgt atttacgcgc tgatgtcgaa cgcccaattg tgccagcaga
     8281 agtaatatgc aaattagctg cggccgaata agtacgtcta taaattgagt tagaagctat
     8341 cctatctcct gcatcatctg aacctacaga cagtaggtct gtactctgta tatgaatata
     8401 cctattacca tcacgtcgtt tcagcatatt aaatttgcca taccctgctt cgattgttgt
     8461 atctccacct gttgcatatc gtccattaac aatttgaaca agacctttat ttctttcttt
     8521 agaaaacctg atacccgcac cgtaatcata gttctcatca gaaccaaaca taatataacc
     8581 gtcactcgaa taagcattat ctgcattaga cagcgtgaat gcaaatcggt ttaatccagg
     8641 cactttgtct gtgtttggat ataaatacac cggtgcctgt ttgcttttga tattcgatga
     8701 agcgtaagac tccagaacaa cccgattatt atctgacgtt agtgcaacga caccaccata
     8761 ggaattgatt gttatgccat tcataccgct atcactgtaa gttttatccc accattgaat
     8821 agtaccggat gaacctccgt cttcgccttc tccatcaata tatgttgaaa taccaaaatg
     8881 tgacatataa agtgaaccgc ctgcggtatt atttctaaac cttagatgtc catctttaag
     8941 acgtgtgaat atatcatcgg ttgatcgttt gcctttccaa gttcgttgca caataccacc
     9001 tagttcaata gaatcattct gtatttgaac atatctgtta ttgttaccgc ctttaattcc
     9061 aattctatta acattgatat caagaccctc tcttgataaa ttaaggctgt tgacaatatc
     9121 ggttttatct actttatctc gcatattttg gataagaagg tttatttctc tattaccgtt
     9181 aatatcaatt ttatcagcat ttaatctaat accacgtggc cccacattta aagcttgagc
     9241 cactccgtta tcatcatatc tgattgttgt tccatctgta acgttttgga caatctcgtt
     9301 taatatattt gaaagtgtac gattggttgc attaaactct tctttagtag ttcttaattt
     9361 gatttcctta ccattttgta taatttgaga accatagcga gtcagtgttc tcctctgtgc
     9421 atctgtgctt tctttgacct tgttgtctgt ataagcatta gctttctttt cagcgtttct
     9481 agcctttagt tctgcgtttt gttttgcctc ttcaagttta gcttgagcat cttgtatagc
     9541 gcgttgctct tcttccgaaa ttttaccatc agcatacgct tgcgattcct tctctttaag
     9601 attatcttga gcatcaatgt atgattttaa agcttcttgc gcttcttgat ttgcttgttc
     9661 aatacttgct ttaatctcag gattattgga caaatcactt aactggtcat cagtatattg
     9721 tttttgttct tccaatccgt ttcgatattc gtttaacgta actttatctt tgatttcacc
     9781 ttttaaagtc gttctctcag cttcagcagt atctaaacgt tcaacaatac cgtctttgtc
     9841 tgttttatag tccgatgttt ttacatagtc acgtaattgt tcttttgtgg attctctagc
     9901 tgcttcaata gctgatttaa caacattagg ttctccgact aactgcaaat cttcattcac
     9961 cgttaaacca aattttgttg ctattatttc caacgcttct ttatattttt catcagtgta
    10021 ttgtgactgt aataatttaa atctatctga aatggcgatt ttgacatctt ctacatctgt
    10081 ataaacatct tgtaatttct ttctatactc aagaaataaa gtttttgtat ctaccaaccg
    10141 accaatcgtt gcagtttcgg gtgtcataga ttctaaatta tttttaattt gattataaac
    10201 atcaatcaca gcgtctaaac ttgcttgtaa gtccgctttc aaatcattat ctactaagta
    10261 ctcgctattc agtaattctg tagcttctga caaaagacta gcgtgttgta tagataaatt
    10321 aataaaaata ttgtttaatt cactgaatag cgctttctct cttgttatac cacctaattt
    10381 ttcaacatca tttggtgttg cttcaatcca tcgaccattc caatatctac gcaagacagc
    10441 aacatcaggg ttacttgtat cataccaaag cgtatcattg actggatttt ctggcggtgt
    10501 atcactttta tgaattttgc gttcaaagta ttctaattca ccatctacaa catcttttac
    10561 tatagtattg atattgctaa tattgtcgtt taatttttgg tgtattaggt ttaatcgctt
    10621 gttaaactct tctcgtaatt ctgattcttt gaactcttta ggttgaccga atgtatatgt
    10681 gctattttct gaaattatgt tatattcttc agcaataact tctgcctcta catacaatgg
    10741 cgggttaaaa tctctatgtt ttactctgac tgtatcgcca attgatataa tctcgtgcgg
    10801 atacgtaact tccaaatcag tagaagtaat ctcatatgac ataactgccg acttacgttt
    10861 atttaactct gttttggcta aagaacttaa tcgtgtttca ttcatatttt gatcatctga
    10921 ttgtggttca tatatccccc aaatatagcg cataggtagg ttgaattgac tttgcgcttc
    10981 gtcatctgtc acaactagct ctaaacgctt tcctttgtca ttttcgggtc ccacagcaat
    11041 taatgctgtt ttgatttctg acatatcaat cttcctagtt aacccaacca aatctttacc
    11101 atactcaatt tctttacctt tgaataagct gtttttcttt ttgagtacca catatctacc
    11161 tttgacggta ttagaactaa gctctatata aaaatccaat accattttat aggttgtaca
    11221 taattgcttt aaaacttcat atctagtttg ataagaagtc catgacgtag tacgtaagcc
    11281 atcgtattcg gtttgttcag aaacttccca acctgtatcg ctcaacacat ctttcaatgc
    11341 ttctgaagtt gtctttttct caaatttgcc tggtgcatac ggtttagctg ttgttatatc
    11401 agcaagataa gacgctatac attctatctc tgtgtagccg tccatcgtat cttgaaccca
    11461 gttaataata aattcacgcc attgtttgtt tgaatccctt ataataacac gatgtcgttc
    11521 acggaacttt tcagctcttt ctgatgatat gagcagttca agcatttctg aattgtcatt
    11581 aacattacgt ttatgaatcg ctctaactaa ggaagggtca tcagtagaaa ggaaatctat
    11641 aatcttgtcg ttaaaatcta aaacatgtat cacactctca tctcctttct ataaatatct
    11701 atcttgccat ttaaccgtcg tatcaaagac gttttcaggt tgtatgatta attcactgta
    11761 cccagaatca acattgaaat aattacttcc aaacgatttc tcgctcaaca ttggttcctc
    11821 attgatgaca acactttttg cttgcatatc tatttttact aaatcacctt tttgtataat
    11881 gacatccctt gcgcctttcg gttttggtag aatctccgta ttgaatgaac ctaatccatt
    11941 catctccatc cacttataac cattatactt cgcactatag atagctatga tagaagctgg
    12001 acgctgataa aacttaccgc catctatcca ctctttctca tccatatcaa taggtttacg
    12061 tctatctggg tctttaatgt gatcaaattt ccaagtttta atagaaaatt tattacctac
    12121 tcttctgagc cgcatataaa caacgattct gtccaagtta tacattatcg gtttattctg
    12181 atagtcgtat atctttttgg ggtctccttt ttggttatac aacgtaacaa caatatgtcc
    12241 tatttttcta tcatgatatt tattttcata accaatagaa gcaagtaact taccatcact
    12301 atcataaata tgttgtgctg ttcttccggc acctttacct ttttgttcaa caatacattt
    12361 ataggtaatt tgaaaatctg tcatcgcttt agggagccct cgtttcgtgc cagcaccaac
    12421 ccaacctttt gcatcagaaa aattagttgc tttatatcct tcgccaagat tggatatcac
    12481 aaagtcaccg ccgactttac cacctaaatc attacttgga atatcttcag taatcatctt
    12541 agtccaacct ttgaaatcac gaaactcact atgataaaca ggaggcatgt aatccttaac
    12601 ttctttggtt acctcatcat caccaaccat aaaataatct tcatcatttt tagtgatcat
    12661 aaagtaacta gatggtttaa ttgctcgggc ttcaacaatt aaaggagtgt cagcagtccc
    12721 actatttaca actgaaactt ggtctgaaat cgcagtattt ttatatcctg ttactgaata
    12781 tttgtaaggg tctgttagta ctactttgat agtgaactta acaggtattg caaattcttt
    12841 gtgcagcttt attggtcctt caaaataagc gttccagtac caatctttag atttgaattg
    12901 taatttaact tgttcctcgt agttaaaaaa ctttactaat tcattcaaga cgtcatcatg
    12961 tgttttaaag ccgttgtgag ataaatagtc attacgtacc accaaaggta tatcaaaact
    13021 ataagattca agcctacgcc ctttatatat agaccccgaa cgtccatcta cattttcagt
    13081 ttttaaaaca taattaaaag agggtatttc aaaccctctt tcgacataca accaaggaat
    13141 tgttttgttg ttcactttaa tagtgtctat cattgaatag caattcctcc ttttctaaac
    13201 tttacttttg ttgattcttg cctttctcgc ttttctatag acgcgttcac ctttttatca
    13261 aaagcgtatt cgtcaataat cggctgataa tctttatctg caatcacatc gttggattgc
    13321 gctattttca gtaataaagc tatttgttgt tgctgttgtt caatcatttt caataataag
    13381 cttgggtcat caaacccatt tacactagac aattgactag gacgcttatt tttactcgct
    13441 tttctccctc ttacttctgc tgctgcataa tgcaacatct tcattgcatc atttctacga
    13501 gctggatctg ttggaataat ccattctgga tgaccgtctt cacctaagtt ataccaacca
    13561 tcaaaaactt ttccacctgt agcatatgcg taatcaccag cacgcttaaa tccatcccaa
    13621 ccgtagcgtc taacaatgta ctgcattgct gaaatacctt gatgaaccgg attattaaag
    13681 ttagtatatc cacgtttagc gtttgctcta aaagttgagc cgatgatttg gaataatcct
    13741 cttgatgggt ctcctctttg agcattaata tcccaattat tcactgcatt tgattgatag
    13801 ttactttcac gctttgcaac tcgcatcatc tggtcatgaa tccacttacc tttataacgt
    13861 cctcctaaaa tactttgcgc ttgacggatt actcggcttg cataagttgc accacttcca
    13921 gaagtagcac caccgccacc aattgataac ctaccttttt tctttgcatt tcttaaatat
    13981 ggttcagggt caaagtgacg tccatttctt ctcatttcaa aatgtaagtg tggtcctgta
    14041 ctaaatccgg tattaccagt taaaccaaca acatcgccgg gctttaccat cgtgccacta
    14101 ggtggtgatt tgctaaagtt tttcaaatgc gcaaatagca tatcgataac tccactagta
    14161 atttttacat aattaccata accaccagac ataaatggca ttcttgtaag tctgccaccc
    14221 atcggcgttc taacttcttg atatacaaat ggaaaatcga caccttcatg aaatggtctt
    14281 ccagttgcag cagtataagc tgcggtacgt ccataatgat aattaatttt gtcagggtct
    14341 aatattccgc cgactaaatc gccaccgccc atagcttcta aattttcttt tatccaatca
    14401 gtagcacttt tcttaatctt agaccatgca gcttttgtta tgtcgcctgc aattcccata
    14461 cctttagtta aagaattgaa atcaattcca aaagcttcaa gtatataatt taaaagtttg
    14521 ccaggatttt ccataaaatc taaaacatcg ccaactttat cgccaagcca tttggtacct
    14581 ttacctattt gatcttttgt ccagttaaat gccgatgatg caccggattt aatatctttc
    14641 cacatagtac ctaagctaaa tcttggaagc gttccgttta acattgaata agtttgtgta
    14701 ccgttgtata cttttgagcc tttaggtaaa taagcggtag tatctgtatt aggtgtgatt
    14761 acacgtttac cattagggaa ttcaattatt tcatttctga aaccattcgg accatttcca
    14821 cgtcctttat ccccaaccgt agcgaacgta tcccgcgcaa tcttaccgtt cttaactaat
    14881 cttgtagtag tatgtgtgtg ttctgtacca gtgtgtaact taggtatttt atccattccc
    14941 agcttaccac cgacccagtt taaaccttca attaatttat taagtccttt tttaatagca
    15001 tctaccatac cgccgatatg acttttaatt ttgctgataa tactttgtaa gccgtcacgc
    15061 atatttgtaa aaattccacg cactttactc cataaacgac ttgcaattcc aaccgtatta
    15121 tctttaatgg aattccaaat atttgccatc caatttctta gattactaaa aatatctttt
    15181 gtcgcattcc acaaactagt gaattttgat ttcacaccac taaataatga ctgtgctttt
    15241 cctatcgtat tcgtacggat actactccat gtgttagata accaattttt catattcgt
//
LOCUS       DANRDI010000053        15203 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.053, whole genome shotgun sequence.
ACCESSION   DANRDI010000053 DANRDI010000000
VERSION     DANRDI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.053"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            108..1394
                     /gene="rseP"
                     /locus_tag="PZ377_001290"
     CDS             108..1394
                     /gene="rseP"
                     /locus_tag="PZ377_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDM6358167.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1414..3117
                     /locus_tag="PZ377_001291"
     CDS             1414..3117
                     /locus_tag="PZ377_001291"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDM6358168.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            3381..7691
                     /locus_tag="PZ377_001292"
     CDS             3381..7691
                     /locus_tag="PZ377_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDM6358169.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            7981..8448
                     /gene="rimP"
                     /locus_tag="PZ377_001293"
     CDS             7981..8448
                     /gene="rimP"
                     /locus_tag="PZ377_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDM6358170.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            8469..9644
                     /gene="nusA"
                     /locus_tag="PZ377_001294"
     CDS             8469..9644
                     /gene="nusA"
                     /locus_tag="PZ377_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDM6358171.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            9665..9949
                     /locus_tag="PZ377_001295"
     CDS             9665..9949
                     /locus_tag="PZ377_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDM6358172.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            9946..10263
                     /locus_tag="PZ377_001296"
     CDS             9946..10263
                     /locus_tag="PZ377_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HDM6358173.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            10268..12385
                     /gene="infB"
                     /locus_tag="PZ377_001297"
     CDS             10268..12385
                     /gene="infB"
                     /locus_tag="PZ377_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDM6358174.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            12771..13121
                     /gene="rbfA"
                     /locus_tag="PZ377_001298"
     CDS             12771..13121
                     /gene="rbfA"
                     /locus_tag="PZ377_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDM6358175.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            13290..14207
                     /gene="truB"
                     /locus_tag="PZ377_001299"
     CDS             13290..14207
                     /gene="truB"
                     /locus_tag="PZ377_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDM6358176.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            14222..15193
                     /gene="ribF"
                     /locus_tag="PZ377_001300"
     CDS             14222..15193
                     /gene="ribF"
                     /locus_tag="PZ377_001300"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDM6358177.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
BASE COUNT         5546 a         2052 c         3028 g         4577 t
ORIGIN      
        1 acgttatatt ttgtgatttg tcagtatcat attataatga cttatgttac gtatacagca
       61 atcattttta aaataaaaga aatttataaa caatcgaggt gtagcgagtg agctatttag
      121 ttacaataat tgcatttatt attgtttttg gtgtactagt aactgttcat gaatatggcc
      181 atatgttttt tgcgaaaaga gcaggcatta tgtgtccaga atttgcgatc ggtatggggc
      241 caaaaatttt tagttttaga aaaaatgaaa cactttacac tattaggtta ttgcctgttg
      301 gtggatatgt tcgtatggca ggagatggct tagaagagcc accagtcgag cccggtatga
      361 acgttaaaat taaacttaat gaagaaaatg aaataacaca tatcatatta gatgatcatc
      421 ataagtttca acaaattgaa gcgatcgaag ttaaaaaatg tgattttaag gatgacttat
      481 tcatagaagg tatcactgct tatgataatg aaagacatca ttttaaaatt gctagaaagt
      541 ctttctttgt tgaaaatggt agcttagttc aaattgctcc gagagacaga caatttgcac
      601 ataaaaagcc atggccgaaa tttttaacat tatttgcggg accgttattt aactttatat
      661 tagctttagt cctatttatt ggtcttgcat attatcaagg cacgcctacg tctactgtag
      721 aacaagtcgc agataagtat ccagctcaac aagcaggatt acaaaaaggt gataagatcg
      781 tccaaattgg caaatataaa atatctgaat ttgatgatgt tgataaggcg ttagataaag
      841 ttaaagataa taagacgact gttaaatttg aacgtgatgg taaaacaaag tcagttgaat
      901 taacacctaa aaagactgaa aaaaaactga ctaaagtaag ttcagagacg aagtatgttc
      961 tcggattcca accagcgagt gaacatacac tttttaaacc aattgtattc ggatttaaaa
     1021 gctttttaat cggtagtact tatattttta cagctgtagt aggtatgttg gctagtatat
     1081 ttacgggcgg attctcattt gatatgttaa atggtccggt tggtatttat cataacgtcg
     1141 actcagttgt taaagcgggt atcattagct taattggtta cactgcgtta ttaagtgtaa
     1201 acttaggtat tatgaattta attcctattc ctgcactaga cggtggtcgt attttatttg
     1261 ttatatatga agcgattttc agaaaaccag ttaataaaaa agcggaaaca acgattattg
     1321 ctattggtgc cattttcatg gtcgttataa tgatattagt aacgtggaat gatattcgac
     1381 gatatttctt ataatttagg aggataaata attatgaagc aatccaaagt ttttatacca
     1441 acgatgcgtg acgtgccatc agaagcagaa gcacaaagtc atcgtttatt attgaaatcg
     1501 ggtttgataa aacaaagtac aagtgggatt tatagttatt taccgctagc aacacgtgtg
     1561 ttaaataata ttactgcaat tgtgcgacaa gaaatggaac gtatcgattc tgttgaaatt
     1621 ttaatgccag cgttacaaca agctgaatta tgggaagaat caggacgttg gggtgcatat
     1681 ggcccagaat taatgcgttt acaagataga catggaagac aatttgcatt aggtccaaca
     1741 catgaagaat tagttacatc aatagtaaga aatgaattga aatcatacaa acaattaccg
     1801 atgacattat tccaaattca atctaaattc cgtgatgaaa agagaccacg ttttggttta
     1861 cttcgtgggc gtgaatttat tatgaaagat gcatattcat tccatgctga cgaggcatca
     1921 ttagatcaaa cgtatcaaga tatgtatcaa gcgtatagcc gtatttttga gagagttggc
     1981 attaacgcaa gaccagtagt tgcagattca ggtgctatag gcggtagcca tacacatgaa
     2041 tttatggcat taagtgctat cggtgaggat acaatcgttt acagtaaaga aagtgattat
     2101 gctgctaaca tcgaaaaagc agaagtcgtt tacgaaccaa atcataagca tactactgtg
     2161 caacctttag aaaaaattga aacaccaaat gttaagactg cgcaagaatt ggcagacttc
     2221 ttaggtagac cagtagatga aatcgttaaa acgatgattt tcaaagttga tggcgaatat
     2281 attatggttt tagtgcgtgg ccatcatgaa attaatgaca ttaaattaaa atcttatttc
     2341 ggcacagata atattgaatt agcaacacaa gacgaaattg ttaatttagt tggtgcaaat
     2401 cctggttcac taggtcctgt aattgataaa gaaatcaaaa tttatgcaga taattttgtg
     2461 caagatttaa ataatttagt tgtcggtgct aacgaagatg gttatcactt aattaatgta
     2521 aatgtaggta gagacttcaa cgttgatgaa tatggcgatt tccgttttat tttagaaggc
     2581 gaaaagttaa gtgatggttc aggcgttgca cattttgctg aaggtattga agttggtcaa
     2641 gtattcaaat tgggtactaa gtattcagaa tcaatgaatg ctacattctt agataaccaa
     2701 ggaaaagctc aatctttaat tatgggttgt tacggaattg gaatttctag aacgctaagt
     2761 gcgattgttg aacaaaatca cgatgataat ggaattgttt ggcctaaatc agttactccg
     2821 tttgatttac atttaatttc tattaatcct aagaaagatg atcaacgaga actagcagat
     2881 gcactatatg ctgaatttaa tactaaattt gatgtgttgt acgatgatcg tcaggaacgt
     2941 gcaggtgtta aatttaatga tgccgattta attggtttac cactgcgaat tgttgttggt
     3001 aaacgtgcat cggaaggtat tgtagaagtt aaagaacgtt taacaggtga tagcgaagaa
     3061 gttcacattg atgacttaat gactgtcatt acaaataaat atgataactt aaaataatta
     3121 agatcgaatg aattataaga gtaggaaaaa gctgaaagaa atctgatgct tatgtcctgc
     3181 tcttattatt tttgatataa tgattattcg atgaaaaatg actgaagaca tagtataatt
     3241 aaagataaat ttgttttaac aatataatga ttagccaaat ataaagcatt taattttcta
     3301 tcattactat gctcacataa tctaaatatt gttcgaacac gtaaaagtaa tttctattta
     3361 aggtggtaat tgtcttggca atgacagagc aacaaaaatt taaagtgctt gctgatcaaa
     3421 ttaaaatttc aaatcaatta gatgctgaaa ttttaaattc aggtgaactg acacgtatag
     3481 atgtttctaa caaaaacaga acatgggaat ttcatattac attaccacaa ttcttagctc
     3541 atgaagatta tttattattt ataaatgcaa tagagcaaga gtttaaagat atcgccaacg
     3601 ttacatgtcg ttttacggta acaaatggca cgaatcaaga tgaacatgca attaaatact
     3661 ttgggcactg tattgaccaa acagctttat ctccaaaagt taaaggtcaa ttgaaacaga
     3721 aaaagcttat tatgtctgga aaagtattaa aagtaatggt atcaaatgac attgaacgta
     3781 atcattttga taaggcatgt aatggaagtc ttatcaaagc gtttagaaat tgtggttttg
     3841 atatcgataa aatcatattc gaaacaaatg ataatgatca agaacaaaac ttagcttctt
     3901 tagaagcaca tattcaagaa gaagacgaac aaagtgcacg attggcaaca gagaaacttg
     3961 aaaaaatgaa agctgaaaaa gcgaaacaac aagataacaa cgaaagtgct gtcgataagt
     4021 gtcaaattgg taagccgatt caaattgaaa atattaaacc aattgaatct attattgagg
     4081 aagagtttaa agttgcaata gagggtgtca tttttgatat aaacttaaaa gaacttaaaa
     4141 gtggtcgcca tatcgtagaa attaaagtga ctgactatac ggactcttta gttttaaaaa
     4201 tgtttactcg taaaaacaaa gatgatttag aacattttaa agcgctaagt gttggtaaat
     4261 gggttagggc tcaaggtcgt attgaagaag atacatttat tagagattta gttatgatga
     4321 tgtctgatat tgaagagatt aaaaaagcga caaaaaaaga taaggctgaa gaaaagcgtg
     4381 tagaattcca cttgcatact gcaatgagcc aaatggatgg tatacccaat attggtgcgt
     4441 atgttaaaca ggcagcagac tggggacatc cagccattgc ggttacagac cataatgttg
     4501 tgcaagcatt tccagatgct cacgcagcag cggaaaaaca tggcattaaa atgatatacg
     4561 gtatggaagg tatgttagtt gatgatggtg ttccgattgc atacaaacca caagatgtcg
     4621 tattaaaaga tgctacttat gttgtgttcg acgttgagac aactggttta tcaaatcagt
     4681 atgataaaat catcgagctt gcagctgtga aagttcataa cggtgaaatc atcgataagt
     4741 ttgaaaggtt tagtaatccg catgaacgat tatcggaaac gattatcaat ttgacgcata
     4801 ttactgatga tatgttagta gatgcccctg agattgaaga agtacttaca gagtttaaag
     4861 aatgggttgg cgatgcgata ttcgtagcgc ataatgcttc gtttgatatg ggcttcatcg
     4921 atacgggata tgaacgtctt gggtttggac catcaacgaa tggtgttatc gatactttag
     4981 aattatctcg tacgattaat actgaatatg gtaaacatgg tttgaatttc ttggctaaaa
     5041 aatatggcgt agaattaacg caacatcacc gtgccattta tgatacagaa gcaacagctt
     5101 acattttcat aaaaatggtt caacaaatga aagaattagg cgtattaaat cataacgaaa
     5161 tcaacaaaaa actcagtaat gaagatgcat ataaacgtgc aagacctagt catgtcacat
     5221 taattgtaca aaaccaacaa ggtcttaaaa atctatttaa aattgtaagt gcatcattgg
     5281 tgaagtattt ctaccgtaca cctcgaattc cacgttcatt gttagatgaa tatcgtgagg
     5341 gattattggt aggtacagcg tgtgatgaag gtgaattatt tacggcagtt atgcagaagg
     5401 accagagtca agttgaaaaa attgccaaat attatgattt tattgaaatt caaccaccgg
     5461 cactttatca agatttaatt gatagagagc ttattagaga tactgaaaca ttacatgaaa
     5521 tttatcaacg tttaatacat gcaggtgaca cagcgggtat acctgttatt gcgacaggaa
     5581 atgcacacta tttgtttgaa catgatggta tcgcacgtaa aattttaata gcatcacaac
     5641 ccggcaatcc acttaatcgc tcaactttac cggaagcaca ttttagaact acagatgaaa
     5701 tgttaaacga gtttcatttt ttaggtgaag aaaaagcgca tgaaattgtt gtgaaaaata
     5761 caaacgaatt agcagatcga attgaacgtg ttgttcctat taaagatgaa ttatacacac
     5821 cgcgtatgga aggtgctaac gaagaaatta gagaactaag ttatgcaaat gcgcgtaaac
     5881 tgtatggtga agacctgcct caaatcgtaa ttgatcgatt agaaaaagaa ttaaaaagta
     5941 ttatcggtaa tggatttgcg gtaatttact taatttcgca acgtttagtt aaaaaatcat
     6001 tagatgatgg atacttagtt ggttcccgtg gttcagtagg ttctagtttt gtagcgacaa
     6061 tgactgagat tactgaagta aacccgttac cgccacacta tatttgtccg aactgtaaaa
     6121 cgagtgaatt tttcaatgat ggttcagtag gatcaggatt tgatttacct gataagacgt
     6181 gtgaaacttg tggagcgcca cttattaaag aaggacaaga tattccgttt gaaacatttt
     6241 taggatttaa gggagataaa gttcctgata tcgacttaaa ctttagtggt gaatatcaac
     6301 cgaatgccca taactacaca aaagtattat ttggtgagga taaagtattc cgtgcaggta
     6361 caattggtac tgttgctgaa aagactgctt ttggttatgt taaaggttat ttgaatgatc
     6421 aaggtatcca caaaagaggt gctgaaatag atcgactcgt taaaggatgt acaggtgtta
     6481 aacgtacaac tggacagcat ccagggggta ttattgtagt acctgattac atggatattt
     6541 atgattttac gccgatacaa tatcctgccg atgatcaaaa ttcagcatgg atgacgacac
     6601 attttgattt ccattctatt catgataatg tattaaaact tgatatactt ggacacgatg
     6661 atccaacaat gattcgtatg cttcaagatt tatcaggaat tgatccaaaa acaatacctg
     6721 tagatgataa agaagttatg cagatattta gtacacctga aagtttgggt gttactgaag
     6781 atgaaatttt atgtaaaaca ggtacatttg gggtaccaga attcggtaca ggattcgtgc
     6841 gtcaaatgtt agaagataca aagccaacaa cattttctga attagttcaa atctcaggat
     6901 tatctcatgg tacagatgtg tggttaggca atgctcaaga attaattaaa accggtatat
     6961 gtgatttatc aagtgtaatt ggttgtcgtg atgatatcat ggtttattta atgtatgctg
     7021 gtttagaacc atcaatggct tttaaaataa tggagtcagt acgtaaaggt aaaggtttaa
     7081 ctgaagaaat gattgaaacg atgaaagaaa atgaagtgcc agattggtat ttagattcat
     7141 gtcttaaaat taagtacatg ttccctaaag cccatgcagc agcatacgtt ttaatggcag
     7201 tacgtatcgc atatttcaaa gtacatcatc cactttatta ctatgcatct tactttacaa
     7261 ttcgtgcgtc agactttgat ttaatcacga tgattaaaga taaaacaagc attcgaaata
     7321 ctgtaaaaga catgtattct cgctatatgg atctaggtaa aaaagaaaaa gacgtattaa
     7381 cagtcttgga aattatgaat gaaatggcgc atcgaggtta tcgaatgcaa ccgattagtt
     7441 tagaaaagag tcaggcgttc gaatttatca ttgaaggcga tacacttatt ccgccgttca
     7501 tatcagtgcc tgggcttggc gaaaacgttg cgaaacgaat tgttgaagct cgtgacgatg
     7561 gcccattttt atcaaaagaa gatttaaaca aaaaagctgg attatctcag aaaattattg
     7621 agtatttaga tgagttaggc tcattaccga atttaccaga taaagctcaa ctttcgatat
     7681 ttgatatgta aaatgaaata atcaaggtat ttatttaatg cgtatggcgt agtcaaagaa
     7741 atacaaaatt gttgctggac acaaaattat gcccgtattt cttttcaatg tcttacgagt
     7801 ctattcaaat gtaatggtga aataaaggaa caaactttta caagaatctc tgattaatag
     7861 tgaagtcatt tgtttcaagc ataaacttat gctataatta agttgcttaa aaattagtga
     7921 actcaggcag aagagtggga gattcccgct cttttctatt tgccaaaaag ggaggcctgt
     7981 atgagtaaaa ttacagaaca agtagaagtg attgttaaac caattatgga agacttgaat
     8041 tttgaacttg tagacgttga atatgtcaaa gagggtagag atcattttct tagaatctct
     8101 attgataaag aaggtggcgt agatttaaat gattgtacgc tagcttctga aaaaataagt
     8161 gaagctatgg atgcaaatga tcctattcct gaaatgtatt atttagacgt agcgtcacct
     8221 ggtgcagaac gtccaattaa aaaagaacaa gatttccaaa atgcaataac taaacctgta
     8281 tttgtttctt tatatgtacc aattgaaggt gaaaaggaat ggttaggcat tttacaagaa
     8341 gtcaataatg aaacaattgt agtacaagtt aaaatcaaag caagaacgaa agatatagag
     8401 ataccgagag acaaaatagc aaaagcacgt cacgcagtta tgatttaacg tgatgaggag
     8461 gaaaaaacgt gtcaagtaat gaattattat tagctactga gtatttagaa aaagaaaaga
     8521 agattcctag agcagtatta attgatgcta ttgaagcagc tttaattact gcatacaaaa
     8581 agaattatga tagtgcaaga aatgtccgtg tggaattaaa tatggatcaa ggtactttca
     8641 aagttatcgc tcgtaaagat gttgttgaag aagtatttga cgacagagat gaagtggatt
     8701 taagtacagc gcttgttaaa aaccctgcat atgaaattgg tgatatatac gaagaagatg
     8761 taacacctaa agattttggt cgtgtaggtg ctcaagcagc gaaacaagca gtaatgcaac
     8821 gtcttcgtga tgctgaacgt gaaattttat ttgaagaatt tatagacaaa gaagaagaca
     8881 tacttactgg aattattgac cgtgttgacc atcgttatgt atatgtgaat ttaggtcgta
     8941 tcgaagctgt tttatctgaa gcagaaagaa gtcctaacga aaaatatatt cctaacgaac
     9001 gtatcaaagt atatgttaac aaagtggaac aaacgacaaa aggtcctcaa atctatgttt
     9061 ctcgtagcca tccaggttta ttaaaacgtt tatttgaaca agaagttcca gaaatttacg
     9121 atggtactgt aattgttaaa tcagtagcac gtgaagctgg cgatcgctct aaaattagtg
     9181 tcttctctga aaacaatgat atagatgctg ttggtgcatg tgttggtgct aaaggcgcac
     9241 gtgttgaagc tgttgttgaa gagctaggtg gtgaaaaaat cgacatcgtt caatggaatg
     9301 aagatccaaa agtatttgta aaaaatgctt taagcccttc tcaagtttta gaagttattg
     9361 ttgatgaaac aaatcaatct acagtagttg ttgttcctga ttatcaattg tcattagcga
     9421 ttggtaaaag aggacaaaac gcacgtctag ctgctaaatt aaccggctgg aaaattgata
     9481 ttaaatcaga aacagatgcg cgtgaagcgg gtatctatcc agtagttgaa gctgaaaaag
     9541 taactgaaga agatgttgct ttagaagatg ctgacacaac agaatcaacc gaagaggtaa
     9601 atgatgtttc agttgaaaca aatgtagaga aagaatctga ataataggtt ggagtgaagt
     9661 atctatgaaa aagaaaaaaa ttccgatgcg aaaatgtatt ctttcaaatg aaatgcatcc
     9721 caaaaaagat atgattcgtg ttgttgttaa taaagaaggc gaaatctttg cggatgttac
     9781 tggaaagaaa caaggccgtg gcgcatatgt ttctaaagat gttgctatgg ttgaaaaagc
     9841 acaacaaaaa gaaattttag aaaaatattt taaagcatct aaagagcaat tggatcctgt
     9901 ttacaaagaa attattagat taatttatag agaagagatc ccaaaatgag tatagatcaa
     9961 atattaaact ttttaggatt agcaatgaga gctggtaaag taaaaacagg tgaatcagtc
    10021 attgttaatg agattaaaaa aggaaatttg aagctcgtta ttgttgcaaa tgatgcgtct
    10081 gataatacag ctaaattaat tacagataaa tgtaagagtt acaaagttcc attcagaaag
    10141 tttggaaatc gaaatgaatt gggaatagca cttggaaaag gtgagcgtgt taatgtaggg
    10201 attactgacc caggctttgc taaaaagttg ctatcaatga tagatgaata tcataaggag
    10261 tgattatatg agtaaacaaa gaatttacga atatgcgaaa gaattaaatc taaagagtaa
    10321 agagattata gatgagttaa aaagcatgaa tattgaggtt tcaaatcata tgcaagcttt
    10381 ggaagatgac caaattaaag cattagataa aaagttcaaa aaagaacaaa agaacgacaa
    10441 taaacaaagc actcaaaata atcaccaaaa atcaaacaat caaaaccaaa ataaagggca
    10501 acaaaaagat aacaaaaaga atcaacaaca aaataataaa ggcaacaaag gcaataaaaa
    10561 gaataataga aataataaga aaaataacaa gaataataaa ccacaaaatc aaccagctgc
    10621 tccaaaagaa ataccatcaa aagtgacata tcaagaaggt attacagtag gcgaatttgc
    10681 ggataaatta aatgttgaat catcagaaat tatcaaaaaa ttattcttac ttggtattgt
    10741 tgctaatatc aatcaatcat taaatcaaga aacaatcgaa ttaattgccg atgattatgg
    10801 cgttgaggtt gaagaagaag ttgtgattaa tgaagaagac ttatcaatct atttcgaaga
    10861 cgaaaaagat gatccagagg caattgagag accagcagtt gtaacaatta tgggacatgt
    10921 tgaccatggt aaaacgactt tattagattc aattcgtcat acaaaagtta cagcaggtga
    10981 agcaggcgga atcactcaac atattggtgc atatcaaatt gaaaacgatg gcaaaaaaat
    11041 cactttctta gatacaccgg gacatgctgc atttacaacg atgcgtgcgc gtggtgcaca
    11101 agtaacagat attactattt tagtagtagc agctgacgat ggtgttatgc cacaaacaat
    11161 tgaagcaatt aaccatgcta aagaagcaga agtaccaatt attgttgcag taaataaaat
    11221 tgataaacca acttcaaatc ctgatcgagt tatgcaagaa ttaactgaat atggtttaat
    11281 tcctgaagat tggggcggcg aaacaatttt cgttccactt tctgcattaa gtggtgatgg
    11341 tatcgacgat ttattagaaa tgataggatt agttgcagaa gttcaagaac ttaaagcaaa
    11401 tcctaaaaac cgtgctgttg gtacagttat cgaagctgaa ttagataaat cacgtggtcc
    11461 ttctgcatca ttattagtac aaaacggtac attaaatgtt ggtgatgcga ttgtagttgg
    11521 taatacttac ggccgtatcc gtgcaatggt taatgactta ggtcaaagaa tcaaaacggc
    11581 tggtccatca acgcctgttg aaattacagg tattaatgat gtgccacaag ctggggatcg
    11641 ctttgttgta tttagtgatg aaaaacaagc tcgtcgtatt ggtgaatcaa gacacgaagc
    11701 tagcattata caacaacgtc aagaaagtaa aaatgtttca ttagataacc tgtttgaaca
    11761 aatgaaacaa ggtgaaatga aagatttaaa cgttattatt aaaggtgatg ttcaaggttc
    11821 tgttgaagct ttagctgcat cattaatgaa aattgatgtt gaaggcgtaa atgttcgtat
    11881 cattcataca gcggttggtg caattaatga gtcagacgtg acacttgcta atgcctcaaa
    11941 tggtattatc attggtttca atgttcgtcc agacagtggt gcaaaacgtg ctgcagaagc
    12001 tgaaaatgtt gatatgcgtt tacacagagt tatttataat gttatcgaag aaattgaatc
    12061 agcgatgaaa ggtttacttg atccagaatt tgaagaacaa gttatcggac aagctgaagt
    12121 tcgtcaaaca ttcaaagttt ctaaagttgg tactattgct ggatgttatg ttactgaagg
    12181 taaaattacg cgaaatgctg gtgtacgtat tattcgtgat ggtattgttc aatatgaagg
    12241 cgaattagat acacttaaac gtttcaaaga tgatgctaag gaagttgcaa aaggttatga
    12301 atgtggtatt acaattgaaa actacaatga ccttaaagaa ggcgatgtta tcgaagcatt
    12361 tgaaatggtt gaaattaagc gttaattaaa taaattacaa gctaaaagta tagttaagat
    12421 tgatatgctc cctataaata ttgcactttt taagtgtcta ctttataggg agcatatttg
    12481 atactagctt ttggtttttt attagaatag attacctatt aaaagttacg ttatatggac
    12541 atgattttgt ataaaatttt gtggtggcct agaatgattt ttaatgacaa aatataatgt
    12601 cgactattat tggaaaattt tctgttgaaa tgcctatctt acggcaaact ttatttgatt
    12661 ttataggctt aatttattaa aataacgtgt gagctaaaat aattgtttaa gcattgttac
    12721 actaaaaaat gcaaataaca attgaactta aagataaaga ggtgacaaga atgagcagta
    12781 tgagagcaga gcgtgttggt gaacaaatga agaaggaatt aatggatatc atcaacaata
    12841 aagtcaaaga tcctcgagtt ggttttatta caattacaga tgttgtttta acaaatgatt
    12901 tatcgcaggc taaagtattt ttaactgtat taggtaacga taaagaagta gaaaatacat
    12961 ttaaagcact tgataaagca aaaggcttca ttaagtctga attaggttct agaatgcgat
    13021 tacgtattat gccggaatta atgtatgaat atgatcaatc aatcgaatat ggtaataaaa
    13081 ttgaacgaat gattcaagat ttacacaaac aagatagata atttagtgtt aggtatctgg
    13141 aaaatgtttg ataatttctt aatatcggta tattaacatt aaacagttaa tacatagatg
    13201 tgtagaaata gttaacattt tccagttttt ttatgaataa atttagttga tacgctatta
    13261 aaatatattt taaaaaagaa ggtgactata tgtataatgg gatattacca gtatataaag
    13321 agcgcggttt aacaagtcat gacgttgtat tcaaattgcg taaaatatta aaaactaaaa
    13381 aaataggtca cacgggtacg cttgatcccg aagttgcagg cgtgttaccg gtatgtatag
    13441 gtaatgcaac gagagttagt gattatgtta tggatatggg caaagcttat gaagcaactg
    13501 tatcgatagg aagaagtaca acgactgaag atcaaacggg tgatacattg gaaacaaaag
    13561 gtgtacactc agcagatttt aataaggacg atattgaccg attgttagaa agttttaaag
    13621 gtatcattga acaaattccg ccgatgtact catccgtcaa agtaaatggt aaaaaattat
    13681 atgaatatgc gcgtaataat gaaacagttg aaagaccaaa gcgtaaagtt aatattaaag
    13741 acattgggcg tatatctgaa ttagatttta aagaaaatga gtgtcatttt aaaatacgcg
    13801 tcatctgtgg taaaggtaca tatattagaa cgctagcaac tgatattggt gtgaaattag
    13861 gctttccggc acatatgtcg aaattaacac gaatcgagtc tggtggattt gtgttgaaag
    13921 atagccttac attagaacaa ataaaagaac ttcatgagca ggattcattg caaaataaat
    13981 tgtttccttt agaatatgga ttaaagggtt tgccaagcat taaaattaaa gattcgcaca
    14041 taaaaaaacg tattttaaat gggcagaaat ttaataaaaa tgaatttgat aacaaaatta
    14101 aagaccaaat tgtatttatt gatgatgatt cagaaaaagt attagcaatt tatatggtac
    14161 accctacaaa agaatcagaa attaaaccta aaaaagtctt taattaaagg agatagaatt
    14221 tatgaaagtc atagaagtga cacatcctat acaatctaaa cagtatatta cagaggatgt
    14281 tgcaatggca ttcggatttt tcgatggcat gcataaaggt catgacaaag tctttgatat
    14341 attaaacgaa atagctgagg cacgcagttt aaaaaaagcg gtgatgacat ttgatccgca
    14401 tccgtctgtc gtgttgaatc ctaaaagaaa acgaacaacg tatttaacgc cactttcaga
    14461 taaaatcgaa aaaattagcc aacatgatat tgattattgt atagtggtta atttttcatc
    14521 taggtttgct aatgtgagcg tagaagattt tgttgaaaat tatataatta aaaataatgt
    14581 aaaagaagtc attgctggtt ttgattttac ttttggtaaa tttggaaaag gtaatatgac
    14641 tgtacttcaa gaatatgatg cgtttaatac gacaattgtg agtaaacaag aaattgaaaa
    14701 tgaaaaaatt tctacaactt ctattcgtca agatttaatc aatggtgagt tgcaaaaagc
    14761 gaatgatgct ttaggctata tatattctat taaaggcact gtagtgcaag gtgaaaaaag
    14821 gggaagaact attggcttcc caacagctaa cattcaacct agtgatgatt atttgttacc
    14881 tcgtaaaggt gtttatgctg ttagtattga aatcggcact gaaaataaat tatatcgagg
    14941 ggtagctaac ataggtgtaa agccaacatt tcatgatcct aacaaagcag aagttgtcat
    15001 cgaagtgaat atctttgact ttgaggataa tatttatggt gaacgagtga ccgtgaattg
    15061 gcatcatttc ttacgtcctg agattaaatt tgatggtatc gacccattag ttaaacaaat
    15121 gaacgatgat aaatcgcgtg ctaaatattt attagcagtt gattttggtg atgaagtagc
    15181 ttataatatc tagagttgcg tat
//
LOCUS       DANRDI010000054        15191 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.054, whole genome shotgun sequence.
ACCESSION   DANRDI010000054 DANRDI010000000
VERSION     DANRDI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.054"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(164..499)
                     /locus_tag="PZ377_001301"
     CDS             complement(164..499)
                     /locus_tag="PZ377_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358178.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(588..1847)
                     /locus_tag="PZ377_001302"
     CDS             complement(588..1847)
                     /locus_tag="PZ377_001302"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDM6358179.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(2313..3173)
                     /locus_tag="PZ377_001303"
     CDS             complement(2313..3173)
                     /locus_tag="PZ377_001303"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDM6358180.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            3391..3912
                     /locus_tag="PZ377_001304"
     CDS             3391..3912
                     /locus_tag="PZ377_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDM6358181.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAIALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(4021..5631)
                     /locus_tag="PZ377_001305"
     CDS             complement(4021..5631)
                     /locus_tag="PZ377_001305"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDM6358182.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(5967..6497)
                     /locus_tag="PZ377_001306"
     CDS             complement(5967..6497)
                     /locus_tag="PZ377_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDM6358183.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(6609..7469)
                     /locus_tag="PZ377_001307"
     CDS             complement(6609..7469)
                     /locus_tag="PZ377_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6358184.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            7893..8696
                     /gene="coaW"
                     /locus_tag="PZ377_001308"
     CDS             7893..8696
                     /gene="coaW"
                     /locus_tag="PZ377_001308"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDM6358185.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            complement(8826..9497)
                     /locus_tag="PZ377_001309"
     CDS             complement(8826..9497)
                     /locus_tag="PZ377_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDM6358186.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(9896..11089)
                     /locus_tag="PZ377_001310"
     CDS             complement(9896..11089)
                     /locus_tag="PZ377_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDM6358187.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(11089..12273)
                     /locus_tag="PZ377_001311"
     CDS             complement(11089..12273)
                     /locus_tag="PZ377_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDM6358188.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            12584..13054
                     /locus_tag="PZ377_001312"
     CDS             12584..13054
                     /locus_tag="PZ377_001312"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDM6358189.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(13206..13556)
                     /locus_tag="PZ377_001313"
     CDS             complement(13206..13556)
                     /locus_tag="PZ377_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358190.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(13570..14871)
                     /locus_tag="PZ377_001314"
     CDS             complement(13570..14871)
                     /locus_tag="PZ377_001314"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDM6358191.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
BASE COUNT         4797 a         2926 c         2075 g         5393 t
ORIGIN      
        1 tatgatacgc tcccttttaa tatgttcaaa ataaaatgct ttaaaatttt gctgcatcta
       61 tttttttata tttttaaata aaaataacta ttcccacatc atcgcaaata atgcgaagac
      121 ttagaaatag ttataatcaa tgctatgttt aaacgtcatg taattattta gcagtacgtt
      181 gatctgttaa atcgacatat gattgcgccc atgcttcaat tggatgtaaa gcctctgcta
      241 acgctttacc tttttcagtt agcacataaa taatttgtac tggactcgta gaaatgattt
      301 gcttctcaac taactcccat tgtgcaagct ctgacaactt aagacttaaa gcacgtggtg
      361 ttattgtttt caaatctctt ttcatatcgg aaaagtgtgc tgaacagtca ttacatcttg
      421 agagataatt aatgattaat ccattccaac ttctaccaag tattttaaaa gtttcttcga
      481 gatacggaca tacttccatc atcttcacct ctcatcaata aatttagaat gtaaacgaac
      541 accatttttt aatcagtata attattatac cacaaaacaa gataaatcta tacagtttcc
      601 gtccaaatat ctgcacctaa agcttttaag tgttctacaa tatctgtata acctctataa
      661 atatgtttaa cattgtaaat tgtagttaca ccctcagcaa ttaaaccagc aataattaaa
      721 caagctcctg ctcttaaatc gctagcataa acttcagcac catgtaatgt tgatggtttt
      781 atcgttgctg tgccttcgtc aacttcaata tttgcaccca tgcgctttaa ttcttcaaca
      841 tgtttaaaac gctccggata aatcgtatca gttacaaatg aaggaccatt tgccataaat
      901 aataatggtg taataggctg ttgcaaatca gtagcaaaac ctggatatac tagtgtttta
      961 atatcaacaa attgatatgg cgcattatta ttgatgcgaa ttctttcgtc tcttacatca
     1021 acattcacac ctaattcact aaatttagca gttaatgttt ctacatgttt cggaacaata
     1081 ttatttaata taacattttc tccacatgct gcagcgatac acatatatgt gcctgcttca
     1141 attctatcag gtataacttg atactcagaa ccatgtaatt ctttgacgcc attgatttta
     1201 attgttgatg tacccgctcc cttaatatta gctcccatac ttgttaagaa gttagcaaca
     1261 tcaactactt ccggttcttt agcagcattt tcaattacag tttgtcctgt tgcataaact
     1321 gcagctagca taatgttaat tgttgcacct acgctaacca tatctagaaa aatatgtgca
     1381 ccttttaact ctttagcttc gattttcatt gatgtcgtac ttgattcatc aatttcagca
     1441 cctaaagctt taaatccttt aatatgttga tcaattggac gtggcccaag tggacatcct
     1501 cccggtaaac caatcacaca ttttttaaat ctacctaaca tggcacccat catatagtat
     1561 gaagcacgta atgattcaac tttattattt ggtaatgcag cattttgtat ttcagttgta
     1621 tcaacttcta attccgtacc atttagtgat gccttaatat ttaaatcttc taataaactc
     1681 actaaagttt taacatcaga gatttgcggt aacccttcta atttcacatg tccttgagct
     1741 aataatgttg caggaatgat agctacagca ctgtttttcg cgccactgat attaacttcc
     1801 ccatttagtg tgcgtccacc tcttattttt attacctctt gagccatatc atttatctcc
     1861 tttgtccatt actttttaat tttttgcatt attagcatta taaattaatt ttactttatt
     1921 tgtaaaatag tttattttaa tcgttaaaat cttgtctttc tattttcgat tgattaataa
     1981 caaatcattc acctttacta atatatgctg ttcttcccca cttgaccatg gtctaaagtc
     2041 tgattattca actaaatatt gacgttgttt tgacaattat ttagttttaa ttctacggtt
     2101 ttcacgtaaa cttttttcat aaattgctca ttttccttat aattatatca caatcaattt
     2161 tgatatattt taccattttt acgctttcat tttaaaatca aactgtttta tttattactt
     2221 atacgacata aaaaagaagc aaacaccatt ttgtctgctt cttttaaaat taatatccct
     2281 acgttattaa taacttaaag actaaatatt aattatttag cgcggttaga agtaccgaac
     2341 tctttaattt tacctttaac tgtttctttg atggcttcac gtgcaggtcc taagtattta
     2401 cgaggatcgt aaacttcttt gtcgttattt aaaacgtcac gaactgcttt tgctgaagcg
     2461 atttggtttt cagtgtttac gttaatttta gctgtaccaa atggaattgc tttttggata
     2521 tctttagtcg ggataccagt accaccgtgt aatactaatg gtaaacctgt agataaaccg
     2581 atttcttcca tttctttaaa tcctaatttt ggttcacctt tgtatggacc atgaactgaa
     2641 cctaatgctg gcgctaatgc atcaatacca gttttttcaa ctagttcttg acattcttta
     2701 ggatcagcat aaatgatgcc gtctgctaca acatcatctt cttgtccacc aacagtacct
     2761 aattcagctt ctacagaaac acctttttca tgagcgtatt caacaacttt tttagttgtt
     2821 gctacgtttt cttcgaatgg gctgtgtgaa gcatcgatca ttactgatgt gaaaccagca
     2881 tcgatagctt ctttacattt ttcaaagctt gaaccatggt ctaaatggat tgctacagga
     2941 atagtgatgt ttaagtcatg cattaaccct tcaaccattt taacaattgt gtagaaaccg
     3001 ctcatgtaac gagcagcacc ttcagaaaca cctaaaatta caggtgcatt ttcttcttgt
     3061 gacgcttcta aaattgcttg agtgaattct aggttattaa tattgtattg acctaccgca
     3121 taaccatttt cttttgcatc aattaacatt tctttcattg aaactaaagg catgaaagtt
     3181 cctccttgag tgcttacgca cgtatttttc aaagtaatta tatcaaaaaa atagcgtcaa
     3241 tgcattgaaa tactgcagat taaatgtgta ttttttgaaa aatgcgatag ttatactaaa
     3301 tgcaaacgta tacattttta tacaatttaa gccgttgcat aatatgtagt cattaattac
     3361 aatattttat aagtagattg gaggataaag atgtcaaaaa tcttaaacac acaactaata
     3421 ggtatcttta ataggctaga aaaacaatct ttagaaattc aaatggccgc ccaatgttta
     3481 attcaagcaa ttggtggcga aggttatgtt tatgtaaaag gttatgatga tttacagttt
     3541 ttcgaaagtt tcattctaca tagtgatgag cgtttaaaat ctagccgaaa acttgacgct
     3601 attaaagact ttaaagaaat tgactcaact gatcgtgtat tattattcgc accattttat
     3661 aatgatcaag ttgcattaga tattcaaaaa cttatcgact tagatatcga tgtggtctta
     3721 ataagcaaca aacctaaaac agatgatttc cctgatcatc tcgtacattt tatcgattta
     3781 tctacaccaa gacctatagt ctacactgaa gattacgata agattgtcca accacatgcg
     3841 atagcattga attatgtata ctatgacatt tatacacaaa tgattgaaat gacaagagac
     3901 ttagaattat aaaagtcact attgttattt cataccttgt cactaacata atattaaaat
     3961 tatacgaaaa accggtactt tcaatgttta acaatgaaag taccggtttt attagcaaat
     4021 ttatttattt tgttgatatt ttaatgaagc ttcaataaat gatttaaaaa tcgggtgcgg
     4081 acgatttggt ctagataaga attctgggtg gaattgacaa gcaataaaga aatcatttgt
     4141 cggaatctct accatttcta ctaaacgtcc atctggactt gtaccagaaa tcaccatacc
     4201 atttgcttct aattgttctc tatagtcatt attaaattca taacgatgac gatgtctttc
     4261 ttcaatttcc gctttaccat aaacatcttg tgccaatgtg ccttctttaa ttgaacatgg
     4321 atataagcct aagcgtaatg taccacctaa atcttcgata tctttttgtt ctggtaataa
     4381 atctataatt gggtatggtg ttgctgggtc taattcagct gaatgtgcgc cttcaaggcc
     4441 taatacgttt cttgaaaatt caactgttgc aagttgcatt cctaaacaaa taccaaagaa
     4501 tggtacattg ttttctctag catacttaat tgcactaatt ttaccttcac ttgcacggaa
     4561 accaaatcca cctggtacta aaataccgtc gacatctgca aggtattcgg ctgcattttc
     4621 atctgttact tcacttgaat caatccatct aatgtcaata tctttggcaa aaggatatcc
     4681 agcatgtttc aatgattcaa caactgataa atatgcatct tgtaagctaa catatttacc
     4741 tactaaacca attgtaattt taccatctaa attattaacg atatctaaca actgtttcca
     4801 ttcatcaagc tgtgtttcat atttcgcgtt taattgtaaa cgtttaataa cgatatcatc
     4861 catattttgt tggcttaatt gtaatggaat ttcgtataaa gagtctgcat cacgacattc
     4921 aataacactt tctttattaa tgtcacagaa taatgcaatt ttatctttta aatcttgtgt
     4981 catttcatat tcagttctta caacgattaa gtctggttga atacctaagc ctcgtaattc
     5041 tttaacacta tgttgtgttg gcttcgtttt catttctcca gcagctttaa tataaggcag
     5101 taatgtacag tgaacataca taacattttc tctacctaaa tcgctacgaa tttgacgaat
     5161 cgcttcaata aacggtaatg actcaatatc acctgttgta ccgccaattt cagtgataac
     5221 aacgtctgca ttcgtacttt cccctgcaag taataaacgt tctttaattt catttgtaat
     5281 atgcggaata acttgaactg ttccgcctaa gtaatcacca cgacgttctt ttttcaatac
     5341 gtgtgaatac actttaccgg ctgtcacatt tgaaaacttg tttaaattaa tatcaataaa
     5401 tctttcgtaa tgtcctaagt ctaggtcagt ttctgcacca tcatccgtta cgaatacttc
     5461 accatgttga taaggactca ttgtacctgg gtcaacattt aagtatggat cgaatttttg
     5521 aattgttaca tttagacctc tatcttttaa taatctacct agagaagatg ctgtaatacc
     5581 cttccctaat gatgaaacta cgccacctgt tacaaaaata aattttgtca tgtcttgacc
     5641 tcctaattat taaagggaat gcttcctcat caattgtaaa aattacgtaa aacataacat
     5701 taaagcaagc tcattcaagt tgtttatttg tcgaattcga caaaattaaa ttctgtttac
     5761 ttaccgtttc catactgcta tgtcactatg tatatcattt acaactttga tttttgcgtt
     5821 atcatttctt gatttactca attaaataaa aaaacgctcc ctctcacaaa ttctgtaagg
     5881 ggagcgtata aattttatac acgtgtccta tttaaaggag cccgaataaa attttatcat
     5941 ctttcaacta aaagtcaaac aaaaaattaa tcgttgaagt cttcttcgtc ttcaaacact
     6001 tcttcgtctt cgtctagttc atcttcatct tcttcaatga ctatatccga atgattgatt
     6061 tcttcttcaa cctgctcatc ttctggatca tttagttctt cttgatcatc tgtttgagct
     6121 ggaatatcat cgtcgtcatc catttcatct tcgcccaata attttaagtt ttgatcttct
     6181 tcatcatctg catccagaat atcgaatttt tgaatagttg gtgcgatttt ctcttcaata
     6241 tcatctaccg aataccaatc acgtaatccc cataaatttt ctccaacatt taaaaaacga
     6301 ccatctgtgt ttaaatccgt gtaaaattgt acaacacgat tttcaatttc ttcgtactca
     6361 taatcaccta acgctctaaa ttcatcgatg atatcatata agttcattgt ttcgccttta
     6421 tcattcaata atgtataagc catatcaata aatgattttt catcaaccat ttgttttgta
     6481 taatcttgaa ttttcattgc ccaatacttc ctttcgaagg atataatcta aatttatacg
     6541 tctgaattca tttatatata gtaacaaaac taaattataa atgacaatat catatttcgc
     6601 aatcactgtt agtaatttaa atatttttcg aaaacgacat aatcatcagt ttgttcgatt
     6661 tcaacgaaat tactatttaa acataatggt ttaaaatcat tattctctcc aaacagaatc
     6721 atcgtaatca cattaaggtt gtcatagttt tttcttaagt aagttggcaa ttgtcgcaga
     6781 gcacttgaac caataccatt aaagcgatat gatttatcca cttcaatagc agcaatgttc
     6841 atgcgttcac caatctgctc caatgcgata aagccaacct tttggccatc aatcattaaa
     6901 ttaatgatat gtacattggt cacttctgaa cgactttctt cttcaagctt taatgatgaa
     6961 acatgttttg aatctaagtc caagtaatat agtcgttctt tgccaattgg tccttcttct
     7021 ttagtcgctg tatgcatgaa tccagcacgt tcgtataggt tccaagcatt gtcattttcc
     7081 gcatctacta ctagatatag atgattaaaa tcaggaaata caccttgaac atattgcggc
     7141 aatgacatca ttattttcgt gccatatcca aaaccttgat atttttcatt aatcgataat
     7201 gaacgaatat aaacgacatt ttcaggtgta tcataacctt catgctgata gtaacgatgt
     7261 aatacaaaaa agcccaccac ttcattttta tcgtttattg ctacgttagc aatcctatca
     7321 gcatcattaa ttgcatcatc aataacttct ttaggtaaag atgaatatat ttgttgtctt
     7381 tcattcaagt caaaatcatt aattgctgaa cgatattgtt catcaaatgc ttttaaagtg
     7441 ataccttcaa aacaaagttc tttagccata tatttaaccc ctatcttttt atatatgaag
     7501 catgaatccc catattattt tacctattat ttcactacga taccattatt tgctctaagt
     7561 taaataaatt acttatttat attttttaaa tttcaaaatt aatgtactat tcctcattac
     7621 ttatcatttt aatcagagac ttacaaatac actgcgtacg aactactttt aatattgcgt
     7681 cactaactaa caatcctata caacttcaaa attcctagaa aataatatat ataacttaac
     7741 gtccacgttt gtaaaacatt aattgcgata cgcttacaat gttgaaatgc aatggcatca
     7801 aatttgttaa taattactat tattacttgt tttttagtca ttttactata ctattattta
     7861 aggatagaca ttaataatga ggtgggaatc ttatgaaagt tggcattgac gctggcggta
     7921 cactaattaa aatcgttcaa gagcaagata accaacgtac ttttaaaact gaattaacta
     7981 aaaatattga tcaagttgtg gaatggttaa accaacagca aattgaaaaa ttatgcttaa
     8041 ctggaggtaa tgcaggtgtc attgctgaaa acatcaacat tcctgcacaa atttttgtag
     8101 agtttgatgc tgcatctcaa ggactaggta ttttgttaaa agagcaaggc catgacttag
     8161 cagattatat ttttgcaaat gttggtaccg gtacgtcact acattatttc gacggccaat
     8221 cacaacgtcg tgtaggcggt atcggtacag gtggaggcat gattcaagga ctcggttatt
     8281 tactaagtca aatcactgat tataaacagt taactgacat ggcacaacac ggtgatcgta
     8341 acactatcga tttaaaggtt agacatattt ataaagatac cgaaccaccc attcccggtg
     8401 atttaacagc agctaatttt ggtcatgtac tacatcattt agatgctgat tttacaccta
     8461 gcaataaatt agcagccgtt attggtgtcg ttggcgaagt tgttacgaca atggccatta
     8521 ctgttgcacg tgaatttaaa actgaaaata tcgtttatat cggttcttca tttcataata
     8581 acgctttatt acgcaaagtt gtggaagatt atactgtgtt acgcggttgc aaaccgtact
     8641 atgttgaaaa cggcgcattt tcaggtgcta ttggtgcatt atatttagaa aaatagaaaa
     8701 agaccattta tatgcgcgtt gttttaaata aacgctatat aaatggcctt ttattttatg
     8761 gtctaagtgt tttgaatacg tattgttcca tttattatca cttaattact tacaactcaa
     8821 ttcaattaaa atgtaagttc attagacaat ttttgcgctg aaacaattgt accaaccacg
     8881 ccagttttta aatctatcgc aacggcttta tgatcatctc taagttcatc tagccattct
     8941 ccgaaaacag aaccttcaat cgtaattaaa tcacactctt caaaatcttc atctcgaact
     9001 tttaacgctc tgtttttaat actccataca ggcataatat gcggcttttt ttcgtcgcta
     9061 tcattttcac taatgagaat aaactgctta ctaccttttt tagttaagcc gtatacttca
     9121 tcatatttcg ctacatcttg gacaaattct ttgattctaa ttttatctaa ttccgtcatc
     9181 acttcttgtg taacagctac aacgtttagt agatgccctt cttcttccat tgtttgattc
     9241 ataataatat ggtcttgttc atcaaacaat tcatctaatt cataagtagc aaaacgatca
     9301 acgtccattt ttacaacttt atcaaatgga atatcacgac ttgttaaaaa tgatacggcc
     9361 acactttctt ctgtccaaac gcctacatac gcttcacttt tataatttag gcgtactagt
     9421 tttttatttt tagaggcgaa ataaatacat tcatttatta atatatctct aaagaatgga
     9481 tatgctttat aagacatgct tgaccctcac tttttaaaat atgtcataca taaaatagta
     9541 aatgactcca acaagttagt caattagaca tattatgaat tcaatttaat gtcatgtttt
     9601 tcattttaaa aattcaatag cattaaattt tacgtataca caaattcggt ctataacacg
     9661 aaacatatct agttactaaa aatttcaaaa cgataacctt tagtatacct ttatcaacaa
     9721 aatggcaact cttataacat agtgaaataa aagtcgccat ttaatattag tattacaaag
     9781 tattgccata tcataaattt aattactgtt gactagaatc gaaaaaagct tgttacaaac
     9841 gcatattcag tcaactactg gcaacacaac attgtagcgc cttatgttcc gaactctaat
     9901 agtgttcaag ttcagctaaa aatgcgcgtt cattctccag tactgtttct tttgaatcac
     9961 catgccaaat acagccaaac cttgatttaa ctttatcttc accgtaccaa tcaccatcat
    10021 aataagataa cgggtataaa ctaccttctt tgacatattt caaaatcgta ttgaagaaat
    10081 gtgtgttatc accttttgaa tgataactat aaaacttttg atatcctgaa ttcaactcgt
    10141 tagcaagtaa taacatgctc gttgaaccat tttgtctaaa gtttaaatca atcgcataaa
    10201 cgttatcatc ctcatcgacg agtaggtcaa aaccagctac accaaagaat ccttgattca
    10261 caccgttttc cataatttgt ctacctgctt ctataacgtg ctctggtaca ttagtagtat
    10321 tttcatttcc gttataaaat ccgtacttat ccgttaattg cgttgccgca ccaagatatt
    10381 gaatgcctag cgattccgag tatgcaaact gaacgcaata attcgctttt tcttcaatct
    10441 tctgttcgat aataagcgaa ttcgtttcag cagttgcctc tttaatacgc gttatcgctt
    10501 tttgtaaatc tgcatcatga taacaaatca taacgccata gccacctgct gtcggaagat
    10561 catctcccgg cttaattact aacgggaatt cccaattctt aatctcgttt tcgaattgct
    10621 caatttttac aacttttctt tttggtaaaa acttcccatt tgtccattca ggtattcttg
    10681 ctttattatt taaagcaaca aataacgttt tatctaatgc ataatattgc tgattcaaga
    10741 ttgtttcatc atgaatatat tgaaaataaa tctttttatt ttccttatgt gccaattgtt
    10801 tgatcaagtt ttcgtaagat tgctgattgt taaatgtata aattgagttc ggtacttcct
    10861 taccaataac ttgaaatagc tgatgcaatt tgtctgtcgc actagcttcg tgaacaataa
    10921 caggtaattg atttgctatt aataactccc taccagttaa aaaattagat tgatgttcgt
    10981 ccggtttcaa ccatggattc gatatatacg aaggtcttga cgtatagaca acatctttgt
    11041 catataaatc acttaacgtt aagttcggct cattaccatt atttgtcatt acttcccatt
    11101 ccctttcaaa tgcgcatgct cttcaataat gtcttgataa acgtcttgat ttgtaattaa
    11161 ctctaacccc atcaacgcca ttattttagc gcctttaatt aatgcttcat caccatgtac
    11221 actcgcagcc gcttctctaa atctatgcgt atgtcctact aaattacgtg atcctatttt
    11281 aatatgagga tgtattgttg gcacaacatg acttacgttc cctgtatccg tagagccata
    11341 accaaaatca tcatcaataa ctgcttcacc aacttcttca gcatatttag caaataaatc
    11401 atctaatttc ggcgttttaa tgaattcatt cacaccgttt tgaattcgac caaattcata
    11461 atcacaacca gtctgtatcg cagctccacg tgcgatttga tttacttttt ctgttaatat
    11521 atccaattct ttacgcgtca ttgctctagt ataaaaacga gcatgtgtat agtctggaat
    11581 aatattagct gctttcccgc catctaaaat cacaccatgc acacgttgat cttttttaat
    11641 atgttgtcgt agttgtgcta caccattaaa ataactaatc atagcgtcta atgcatttaa
    11701 cgcttcatct gcattttcag aggcatgagc actttttccg taaaatttaa catctaaaac
    11761 atccactgcc aaagtatcaa tcgttttata agtttcattt cccggatgaa tcattaaggc
    11821 aatgtctatt tgatcaatca caccagcctt gacataagaa gctttagcgc taccattttc
    11881 cccaccttct tcagctggac atccaagaac gactacttta ccaccaattt ggtcaatcac
    11941 ttgcttcaaa ccaattgcac caagaacact tgcagttcca atgatattat gaccacaagc
    12001 atgacccaat cctggcaaag catcgtattc tgctaaaaaa cctatagctg gcccgtcaag
    12061 tcctgaatca tatgtcgcta taaacccagt tgcatgccca gcaatctcgg tttcaatttc
    12121 aaaatcatgc tctttcaaac gatctattaa agttcgagac gcaaatattt cttcattacc
    12181 aagttcagga cgttcatgaa ttctatgact gatttcgata taactatatt tatttgtttc
    12241 tatataatcg agaatttgtt gtttttcact cattttttac tatctccctt ttaccctaca
    12301 ctcattttaa tcatccacaa tattttgttc tttcaaaatg aattattact tattctatcg
    12361 gttttatctc atgatgtcat ctagtttttc tttatttaca aaattttcta ataactaaaa
    12421 gcctttcata aatttataaa acagtttcaa attgtaaaac ataagcccta ttgttacaat
    12481 ttaagtattg ttagaaacac aacttacaac aaacttaagt tttataatgc aacaaatcat
    12541 aagcgtttta tagttttgag tagttaatag gaggaaattc aaaatgacaa aaatgaatgt
    12601 tgaaagtttc aatttagatc atactaaggt ggttgcccca tttattagat tagcgggaac
    12661 gatggaagga ttaaacggag atgtcattca caaatacgac attcgtttca aacaaccaaa
    12721 caaagaacat atggatatgc ccggactaca ttcattagaa catttaatgg ctgaaaatat
    12781 tagaaatcat agtgacaaag ttgttgattt aagtcctatg ggttgccaaa ctggtttcta
    12841 tgtatcattt attaatcatg ataattatga tgatgtatta aatattgttg aagcaacttt
    12901 aaatgatgtg ctaaatgcta ctgaagtgcc tgcttgtaat gaagtacaat gtggctgggc
    12961 agcaagtcat tcattagaag gtgctaaaac tatcgctcaa gcatttctag acaaacgaaa
    13021 cgaatggcat gatgttttcg gtacaggaaa ataaatctta gtcaatcaag ttaatcagaa
    13081 aagcagtcga acaatgattt tacaatcgcc attgtccaac tgctttttat tatgcttcaa
    13141 agtcaaaaaa tcgaacaaat gaaaaagtaa aatctttaac atttgtccga tttatttgag
    13201 aaccactata atttcttaat tagtcccatt aacacgaact gcataggtaa ccttaaatat
    13261 agttgccatg ttggcaattg tttatctcct aaaggtaact ttttaactgc catatagata
    13321 ttagctggga atacagctag caagaataga ttgattgtat ttttcaagca ttgagatggt
    13381 cttttaatta aaagtgcaag tccaaataat atctcaaaga ttcctgtaac aagaaccgct
    13441 gtttttcgaa gtggcaaaca tttcggtatg atatttctaa attgtcgttc tcgtgtaaaa
    13501 tgcaatacac ctattacact aaaacctatt cctaataaat atcttagtat gttcaatcag
    13561 cttcaactcc tattctgtaa tgattttatg aattaatgta ggcgatacaa catgatcagc
    13621 aattgttatg cttgaatcta attttttaac aacatcgtct acatcttggc gattactgtg
    13681 aatcgttaat aatgattctc cttcttctac tttatcacca atttttttat ttaaaacaat
    13741 accaaccgct aaatcaatat catcctcttt tgttaaacgt cccgctccta acatcatcga
    13801 agcgacacct atatcgttag agactaattc agtcacataa cctgattttt tagctttata
    13861 ttcaatttga tattgagctt gtggcaaacg ctctggatgg tcaataacag tttcgtcgcc
    13921 accttggttt ttaataaatg ttttgaattt ttctaatgct gcacctgaat taattgcctc
    13981 aattagcaac gctctcgctt cttcaagcgt ttcagctttg tttgcaagta caaccatttg
    14041 agaacctaat gttaatacaa gttctgttaa atctttcgga ccttgtcctt tcaacgtatc
    14101 aattgcttct tgtaactcaa gcgcattgcc aatcgcacgt ccaagtggct gattcatatc
    14161 agaaataatc gccatcgtat tacgtcccac attattacca atacgtacca ttgcgtgcgc
    14221 taatgcttca gcatcttcta atgttttcat aaatgcaccg ctaccagttt ttacatctaa
    14281 tacaattgca tctgcaccag cagcaatctt tttactcata attgaagagg caattaatgg
    14341 tattgaattg acagtaccag taacatccct taaggcatat aattttttgt ctgcaggagt
    14401 taaatttcct gattgtccta caactgccac tttattttca ttaaccaatt tcacaaatgt
    14461 tgcttcatct atttcaacat gaaaaccatc aattgcttct aatttatcaa tcgtaccacc
    14521 tgtatgacct aatccacgcc cactcatttt tgcaacagga acatctacag ctgctactaa
    14581 tggtgctaaa accaatgtag ttgtatctcc tacaccacct gttgagtgct tatctacttt
    14641 gacaccttta atatcactca aatctatcat atcaccagaa ttaaccatag ccatcgttaa
    14701 tgctgcacgc tcatcatcat tcatatcttg gaaataaatc gccattgcta aacttgatgc
    14761 ttggtaatca ggaatatccc ctttaacata gccgccaata aagaaattaa tttcttccgt
    14821 tgttagtgta tgaccgtcac gctttttctc aataatgtct atcattctca tttttatcat
    14881 ccttttctta aaaagcttag gacaaagcat ctgcgctttc tctagtccat ttttaaaagc
    14941 acaagcgaaa attattatag caagctatcg atttcatttt taatatcaca atttcaatgc
    15001 gatgttatta ttcttaaata gattggttat aacgttaaag tccctattaa attatcttag
    15061 aatcatcatg gcatttatga tgtcttaaag ctgatatcga catacttata tatggttacg
    15121 atgtcccatg cttacatatt tttataaaat tagtaatctg aatctgcttc taaaccttgc
    15181 ataatttgaa c
//
LOCUS       DANRDI010000055        15105 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.055, whole genome shotgun sequence.
ACCESSION   DANRDI010000055 DANRDI010000000
VERSION     DANRDI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.055"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..6592)
                     /locus_tag="PZ377_001315"
     CDS             complement(<1..6592)
                     /locus_tag="PZ377_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDM6358192.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQ"
     gene            complement(7039..8289)
                     /locus_tag="PZ377_001316"
     CDS             complement(7039..8289)
                     /locus_tag="PZ377_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6358193.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(8675..9700)
                     /locus_tag="PZ377_001317"
     CDS             complement(8675..9700)
                     /locus_tag="PZ377_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HDM6358194.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(9873..10232)
                     /locus_tag="PZ377_001318"
     CDS             complement(9873..10232)
                     /locus_tag="PZ377_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDM6358195.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(10449..11480)
                     /locus_tag="PZ377_001319"
     CDS             complement(10449..11480)
                     /locus_tag="PZ377_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDM6358196.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(11493..12254)
                     /locus_tag="PZ377_001320"
     CDS             complement(11493..12254)
                     /locus_tag="PZ377_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358197.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(12251..13225)
                     /locus_tag="PZ377_001321"
     CDS             complement(12251..13225)
                     /locus_tag="PZ377_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDM6358198.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(13239..13979)
                     /locus_tag="PZ377_001322"
     CDS             complement(13239..13979)
                     /locus_tag="PZ377_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358199.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(14321..14833)
                     /locus_tag="PZ377_001323"
     CDS             complement(14321..14833)
                     /locus_tag="PZ377_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDM6358200.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
BASE COUNT         4890 a         2956 c         2205 g         5054 t
ORIGIN      
        1 gttgcccttt atagacatcc gcttctgaaa atgtcacatc ttctgtgtct atatcaaaat
       61 aagttcccac acttatcgta gacacatata ttaaccttgc atgatgttgt tgtgccaaac
      121 gtatgacatc aacagtacct tgaacattta ctttttcaaa ttcatcatca tcaccaaagt
      181 gatctgtacg agcacctgca tgaataatcg tatccatgtt ttctggtaaa acaacatcat
      241 ccatacactc gaaatcacca acaatgactt caatgtttga taacattatt tcaaccgtct
      301 cttctgaaaa ataatcattt aaattcgtca tcaacttata ccatgctatt tcctcattat
      361 cagcacgtat gaaacaataa atgcgatgac tgtatccttg tagtacttca atcagataag
      421 cacctaaaaa acctgtcgcg ccagtcaata gtgtatttcc tagaggtcga tgacttagac
      481 tatcctctaa aatacccaag ttataacgag acataacaat cttttgtaat tccgaaagat
      541 tatccggtaa tgcaactaat gattgttgat tttggtacat ataattaaca atctgtcgca
      601 cggttttata ttggtataat gtctgcattg aaatatgatg gccaaatcgt tttaaatgcg
      661 agacaactaa catcgcctct aatgagttac caccaagttc aaagaaatca tcgtcaacac
      721 cgacatcatt ttgtttcaat acctctccaa atacatcaac aaatgtctgc tcaatttcat
      781 tagagggttc gctatacact ttattagact gttgtatagg tgatggattt ggcaaacgcg
      841 tagtatccac cttgtcattc gtggttaatg gcatacaatc gatatgcgtt atagtcttag
      901 gaatcatata cttaggcagc tgatcattta aatattgctt caaatcctgt tccacttgtt
      961 gctctccgac ataataagca ttcaatatat catgcgtatc aaagtgactt actgttacaa
     1021 cacaatcaga tataccacga atagctaata ttgcattttc aatttcatca agttcaatac
     1081 ggtacccgtt aactttcact tgtttatcta ttcttcctaa aaattcaatt tgaccatcag
     1141 atgtataacg tgctaaatca ccactatgat acaactttcc tttaccaaat ggattatttt
     1201 gccatttatc agccattaat tctggacgat taatatatcc tatcgctaaa ctatcacctg
     1261 caatacacaa ctcgcctggc ataccaatac cgcataacaa accatctgac ataatataca
     1321 cttggatgtt agataagggt ttgccaattg gaatcgtctc aggtatcaaa tcaccacaat
     1381 gatgtgacca atacgatgtg atgactgttg actcagatgg tccataggca ttgaaatacg
     1441 tgccacaatg cttctcaata tatttaacaa aggatgccgt actagttgcc ccgcctgtaa
     1501 tcaacttttc aatataaaag tcttccataa cactacacat ctgtaacgga atcgacgcaa
     1561 ccgtcacacg atgcttatta atgagttgtt gtaactgttc tggattaaca cgttcctctc
     1621 tatctggaat cacaagcgta tgaccattta acaaacaaca ataaatctcc ataactgatg
     1681 catcaaaaac aatatttgca tgttgcaaaa atacttcatt gtcgcctaat tgcaattcag
     1741 ttgaccatgc atgcactaaa ttcaacaaat ttcgttgtcg tatggcaacc cctttaggca
     1801 tcccggtcgt accagatgtg taaatagcat acatctcatt atctaacatc gctgtgtttt
     1861 caagttgatt gccatgtaaa tcatcatatt gttcattttc ctttgattca acaaagcctt
     1921 tagcattttc caattgaatg actggtaatg tcgtttcaat ttcaacgccg taagacatga
     1981 ctgcagttac tttagcatcc tccaaaattg caccttgtcg tttattcgga aaatcaatat
     2041 cgataggtat ataagatgca cctactttaa ctgtcgccaa catcgccgca atcatttcaa
     2101 aactacgttc tgtaaacaag gcaacccgtt gaccattgcc cacaccattt gataggagca
     2161 tgtgcgcaat ggcatccaca tagttgcgta atgtttcata cgtcattgtc aaatcattca
     2221 tgactagcgc aacatgatta ccttgtcgtg agacaacttc attaaagtaa cttatgatag
     2281 atttatttcc cgggacatta agcattcgat cgttaacatg cgtattgacc caatttagaa
     2341 gttcctccgt gccgtttggt atatcacaaa tttgtagtgt atcttgatgc ttcaaaatat
     2401 aatcaatcat aatcatacat tgattaccca tgtgacgaac tgtttctgag tgatataaat
     2461 cggtattata ctcgatattg attgtatagt catcgcgatc ttcttcaatg atgaaagata
     2521 aatcaaattt cgccgtcact gatttgggtt gaatgtgtgt taatttacta tgcccaaaat
     2581 gagcatgatt cgtttcattg ttttgtagta ctaacatgac atcaaataat ggattccgtg
     2641 aggcatcatg tgattgatct aagtcattta ctaaacattc gaatgggtat tcttgatgct
     2701 cgtatgcctc caaactcatt tccttaacct cttgtaaaaa ctgtgtccac attttatcag
     2761 gtgacggttg ccctctatat accaacgtat tagcaaacat gcctagcatt tgctccgtgc
     2821 ctttatgcat acgcgcactc atcacactac cgacaacaac atcatctttt cgagcatatc
     2881 tacttaacaa cgtcatgacc acactcataa agaacataaa atcagtaatt tgatgctttt
     2941 ctacatactt ttgaagtagc tgtctcattt gttgattcat tgtaaatgac atcattgctc
     3001 catttgtcgt tttaatattt ggtctaacat agtctgtcgg taagcttaaa ataggtactt
     3061 catctttgaa ttgagataac caatattgtc tatgtttcgt catatcacga tgcgacatcc
     3121 actcactata gtctttatat tgcaatttaa gtggtaacaa taatttatgt tgataaagtg
     3181 cgttaagatc attcattaat tgtatattac tcataccgtc attaatgata tgatgcgtat
     3241 ctataaagag gtatgcatgt aagggacttc taatgtatct cactctaatt tgacttggct
     3301 tttccaaatt aaaaggtgct acaaattggc gcatgatttc ttgttcatcc gtaaaatgcg
     3361 tgttaacttc ttcaaagtca actgcaacat ctgccacaat acgttgtcga acctcatcat
     3421 ctacaacaat atattgtgtt cgtaaaatct catgtcgcgc tatcaaacgc tgcactgctt
     3481 gtcgcaattg agctacatta agttctgatg ataaccgcca taaaaaaggt acgttataca
     3541 ccgtatcttt atggtttgat ttccataata aatacatacg cttttgtgca gagctcagca
     3601 cataatcatc tttaactata gtttctggaa tcacttcata gttttgttct tgaaccttag
     3661 caatcgcttg tgctagttca aatacagttg gcttttgtaa taaatcacca atttgtaatc
     3721 gtttcccagt agatgcctct atccgattca ccactaacgt tgcttttaat gaatggccac
     3781 ctaattcaaa gaaattatca tgaataccta cttgattcac atgtaaaata tctgcaaaaa
     3841 tttggcatag caagtgttcg gtatctgtac tcggtgctac ataggcatcc gtatcgacat
     3901 agtccatgat aggcaatgcc ttcttatcta atttcccatt aatagtaata ggaatttgct
     3961 caatatgcat gaaattaact ggtatcatgt actccggtaa ggtcatacgt aattgtgatt
     4021 taatcttatt atgtgataat gtatgcatcg cttcataata agcaacgata tactgatctt
     4081 gatcatgatt ttgaacaata acaactgctt tattaatacc ttgtatacgc tcgagcgcat
     4141 gctcaacctc tgacaactca atcctaaacc ctcgaatctt aacttgtttg tcctttcgat
     4201 ataaataatc tatgttgcca tcgggtaaca aacgaacgat atcaccactt ctatacatca
     4261 gctgatttat atttgaatct ttgataaatt tatctgctgt caattctggc tgatttaaat
     4321 aacctgcagc taacccaaag ccacttgtac ataattctcc aggaatacca acgccacacc
     4381 gacgctcgcc ttgcatgata taaacatgag tacccagaat cggtttacca ataggaatac
     4441 gatttggaac tttgttaggt atattatacg tcgttgtaaa tgttgtattt tcagttggtc
     4501 cataaccatt aataatttga ggatgcttcg gtttttgatt aagcaaatcc acccacttag
     4561 cattcaatac ttctccacca attaataaat actttaacgg taccaatact tctattcgtt
     4621 cactagcaat ctgattaaat aatgaggagg ttaaccacat agtattaacg tcattttcat
     4681 tgattaattg ttctaccgct attggattta ataattgttc ttttttagca acaatcagct
     4741 ttccaccatt gagcaatgca ccatatattt caaatgttgc agcatcaaag gctatagttc
     4801 ctgataacaa aatcgtcgtc tcttcattta atggtacata atgattttga tggaccaatc
     4861 gaacaatacc tcggtgcgga attagtgtcc ctttagggtt accagttgtc cccgacgtgt
     4921 aaataacata agcatgatct tctaacgtgt tacatttaga aagattatca atatttttcc
     4981 acgctatctt attcaaatca atgtgattaa tattttgttt accattttca tataaagctt
     5041 ggtacgttat tacaacttta ggcgttacat cttttaaaat gtactcctga cgatcacttg
     5101 gatagttcgg atcaattggc acgtaagccc caccagcttt caacacacct atcatcgcta
     5161 ttatcatctc aatacttttt tcagctatga cagcgacacg atcattaggt tcaacaccat
     5221 actggtttct caaacggtgt gctaaatcat tcgcgcgtgc attcaatgtt tgatatgtta
     5281 taaacactcc gtcaaattgc acagcgacat gattcggcgt tgcttcaact tgttgctcaa
     5341 ataaggtaac aactgtttgc gcatcatcta tctcaggcaa acttaaattg atatcgtcat
     5401 ataattgaat atcacgttct gtcatcaaat taagttcatc tacagttgtt cgtttatttc
     5461 cattttcttc agtaatttgc aaataaatat ttcgaactaa gtcactcagc gtctcgattg
     5521 agagcaaatc atatgctgca ctgttatata taattttgaa cccgtgttga tgtggaaaaa
     5581 tttcaatatc cgctaaagat gtatgtgcat catgtatttg atgtacatcc tcaataacat
     5641 cattacaaca agacatcata tgatgataac aatgaaaaat agtctctagt gaaagcgaag
     5701 acttcgcgca ctgtaattgc gacatatttt gcaacacaca tttattaaaa tctgttgtaa
     5761 aacgttgaca tacatctttt gcatcgattg ttaacgttaa cggcacaata tttccgtgta
     5821 aatcatttgg taaatgtgat ggtacatgta tacctaatgt gacatcatgt tgttgactca
     5881 ttatatgatt agctaaatac acactaacag ccaacgatgc catatctatt gatgtcatat
     5941 catcaatcaa atacgtttga tataaagctt gttcaaatgg atgcttaatt ggaaaataac
     6001 tatcaatatg gatgtcagag ttattctcta accgaaaata gtttgagtct aatgctatat
     6061 gcgatgcatc ttgattgtct ttatcatcat ttctatttat atgtgcatgc tgtcgagtat
     6121 tgttaataac agtattgcca cgatatgcat tgcataaatc atcaagaaaa atatcaattt
     6181 gactatcatc gaaaatggac acatgaaaat ctaatagtat atatgcagca tcagcgaact
     6241 gaaacaattt aactttgaat aaaggtgaat cattaaaatg gtaagtactt aattcttgct
     6301 taaaaaaagc ttctaaatca tagtttgcgg aagaagatgg aacttgtttt atctcaataa
     6361 aaggcagaaa ttcatgaagt atcattgtta aattgtcatc ggtagtaaca tcaaaaaaat
     6421 gtcttataga tgcatgttgt tgcacaattg tcgataatgc atacatcatt ttagtagctt
     6481 caacattttt agcgagttta acccaatacg cattacggtg tgtcgttgat tctgtattat
     6541 ttttgtatat acgaaaatat tcctgttgaa atctcaaatt acccataatc ataaaaagtc
     6601 cttctttcat atcataatac tcattactta ctgaaattgc atgatgatat gataaccgac
     6661 gaaatgttaa ttaactcgtt atgtaatgat taatataaaa caccattcgc aacatatgag
     6721 cgatatattc taccctaaaa tacatcttgt atcatcgtta caattggtat atttttcaat
     6781 gtaaattaca tacatcttcg ataaatagca cactacaact cgttaatcac tttctgttgt
     6841 tcacatctca ttgcaaactc aatattgttg ttacaaaata tccatgaagc aagtttatat
     6901 taaacaaaca actcgcataa aacaattgtt atccttaaat tttaacaaat tcttaataaa
     6961 tttatctcta ttttaattac gaccaaatta ataggttttc catataaaaa gatgcataaa
     7021 ataaatattt aaataaattc aatttgtatt cacttgtttt tgtcccccaa atacaccagc
     7081 aacaagcatg ctagcaccaa ttgttaaaac gataaacata tacagtccca tttgtaatga
     7141 cgttaagaaa acacccaaca caatccctaa cctagctagt gtttctgaaa aatgaatacc
     7201 taatgcatta actgcactat atgttcctct tttagcttta ggaataattt taaagcgttg
     7261 ttctgaaact ataggcgaat aaataatttc acctacagtc gcaattatca taaaaacaac
     7321 taataagcca aactgattaa gataggttag accactatag ccaacaatat aaatcagcaa
     7381 accagtgatt aaagcttttt taaaatctat tttcaataca actttcgaga ttgaatacgt
     7441 gagtaaaatg acgacgaccg tattaatcat tagcaagatt gctaacatct tagcacctgt
     7501 aatatcatat gaacctatac ttattgtttc aaactgatcc tttagtctaa tagcaatata
     7561 tgaggagatt gaaaattcac ccatcatgat gatactgaac cccgaaatca ataacatata
     7621 attacggtct ttcaaaacta atttataact gcgaaatata ttcattattt gtaatttttg
     7681 ataacgactt gcatgcctct tgtcatcact ttgctttact tgatttcggt cttgaggtaa
     7741 ccaaatatat aaaataaaga gtacaattaa aaatatacaa gctgctatta agaaaagtag
     7801 taacatactg tagccataca tcaagccacc taacaatgcc ccaatagcta ccgataagtt
     7861 tgtcatccaa tagctaatct tgtaaatata atgttccacg tcttcggtaa ttgcatccat
     7921 aattaatgtg tccataactg gaaattgtaa tccccaaacg attgtaaata tggcatatgc
     7981 aacacaaaaa ccaataattt gccacaattg atgtgaccca aatacgccca tgaacacaag
     8041 cattatcacc atcgtcgctt gataaataag tactagcaac tttttcggaa atatctcaat
     8101 aaggtaacca gatataatgg acaatggaaa ttttagaacc actaaaccaa caagatatat
     8161 accgacaatt gattgactta acatatctgt taaatatagt gctataaacg gtataaatgc
     8221 tgtcgtaata attagctgta aaaaattgct aatcaatcgt actttcaaag tcgtacttaa
     8281 gcgtttcatt aataactaca cccctttcca catatataca cactaccatt tatataaaat
     8341 ctattattag attatacgtt gaaacaaaga atttttagtt atacaaagtt atttaacaaa
     8401 caaatgcatc gactttacaa tgcatcttgc atataatgcc tcaaaaaaat tagcactata
     8461 tgactatata ttattccaaa tgaatgaaat ttatcaataa ttgcgtaagt gaacacatat
     8521 gtgcgtaaat gttaaatcat tgtcctaaca tagctgtgac tatacttaga tgtaatcata
     8581 aaaaaactac tgccatcttt acaacagcag tagtgcttat atgattcatt tatataaaat
     8641 tgaaagcgtt aatcgctagc ccagcatcct tattctattt agctgtataa cttttactag
     8701 caatacgacc acttgcaaca ataatatctt tatcttggaa aggttcatga ttgaagaaac
     8761 gctctaaaat atctttaact ccatcttcaa tacgtttttg tgcttctaaa gtcatacctg
     8821 aatagtgaac cgtcatagca tttctaggca ttgttctcca tggatgatca gcaggtgcag
     8881 gttgtggata ccaaacatca ccagcatatc cttgtaaatg ctcggatgct aacgcttcaa
     8941 ctaacgcatc gcgatttaca attttaccac gtgcagtatt cactaaataa ctgtgttttt
     9001 tcatacgact taaaacatct ttatcaaata agttatcagt ttctggtgtt aatggtgcat
     9061 gaattgtaat cgcatcactt gttgaaacaa gttcatcaaa gcttacaaat ttagacaatt
     9121 tatggtcttg ttgattgatt ggatcatagt gttgtaatgt tacattaaat ggcgctaatc
     9181 tttcagcaac aagttgtcca attcgaccaa atccaaaaat accaattgtt ttgtgttgta
     9241 attcatgcgc atgattacct acttgagaca agttccattc accttctact gattgacgat
     9301 gaccttcttc atagtttcta agaagtatta ataaatccat aaccgcatgt tctgccacac
     9361 taactgtatt acttcctgta acttcaacga caccaatatt gtgttcactt gctgccgcta
     9421 aatctacatg gtcagatcct acacctgctg taattgctaa tttcaagttc ggtgcttttt
     9481 caatacgttc acgagtcata tatgcaggat aaaatggcgc actaataatc acatccatat
     9541 ctggtaaatg tttatctaag tcttcaccat tatctgctaa tataatgaac tcatgtcctc
     9601 tttcctctaa aaatgttttt aatcctaatg cttttttagt attaagtaat tgattttctt
     9661 gaccttctac tgcttctgga aataatgcta cgattttcat atccatctct cctaaatgat
     9721 aaattttgac ttccttaaag tacgtgttgc atgttgcttg ttttaaaaat atgacaaaaa
     9781 aacggcaccg tttgacatac cacccccttg aattattgtt aaattcaaag cgaagttagc
     9841 aaacagcacc gtgtacctta caccagcaac acttattcta ttgacttatc gaaaacaaat
     9901 attgaaatga cttcatcatc attcaaatca atatctgtaa ataattttac aaacttagcg
     9961 cctacaagac tttccatctc atttacatct atttctttat acatctgttt aatcttctct
    10021 gtgcgtgtat aatggagcat cgattcattg cgtttgtcgt ttaggtaaaa actctcaact
    10081 ttactcaaaa cacctgtcat atgtgcaatc gcccaattat ctttaaacga cactcgaata
    10141 ctattgggtc ctttacccac gtattcttta cgataagatc ttactagatt cgagaattgt
    10201 tgttctttag tatgcgtatc tatagtattc accttcttta aaataagaag ttcattaatt
    10261 gttcacattt tcattataca ccttattaac actttttcaa tgataacgat tattattatt
    10321 acgaaatgta tattttccta gttaaaagga tataacaaat taacagaaaa tgtttataaa
    10381 ctaagattta tattatttta aaaaatgttt caacaaacgc atctaaacaa aaaatcagct
    10441 tatcacactt accccttcaa ctctgcttca agtttaccta aatgtcttaa tgtcggtgtc
    10501 aatgctgcga agaagaatcc ttcttttaac ttgcgaactt gtggcgaata tggtgtgtac
    10561 gctctagaac caccattgac aacagaagct tgatttacat ctaacaataa atagccgatg
    10621 tccttcttca atgatattaa ttcatttaaa tgtgaagtta agttaccgtc atctaatatt
    10681 gcataatatt cctctctaag ttgacgataa cgttttttaa aagcttcaac atcatactct
    10741 aaatattgat ttattccgtt ttgcacattt gaaaatgcat caattaactc taaagaactt
    10801 ttaattgagc ctaatcctat tggaatttgg taagcaataa attgcgggcg aatagttgcc
    10861 gcaaactgct tcgcatcatg cgtgataatt tgtgattgtg gcactacgac ttgattcaat
    10921 gtgatttgat acgttgctga cccgttgact cctaaaaaat tggttttttc aacaagagtg
    10981 ataccatctt gattggcacg tagaatgaac atgacaaatt catctgatga ttcatgtttc
    11041 gaaatcgcac caaaataatg gtcttcttga atattactta cagctggcat acgtccactg
    11101 acaaccaatt gtccatcaac ataagtgtgt tcaaggttca acttttctaa atcattaaat
    11161 gacttcatcg gattagacaa tccggtagca cctaatattt ctccagataa caattgctgt
    11221 tgtaagtcat tatttaaatg tggctgcgtg gcattttcta aatacgttga aaaagctaat
    11281 tggcaccata aacaaaatcc tgttgtcaaa caagactgcg atacagcttc gattacttca
    11341 gcattttttc tcaatgccgc ctcaccgaaa taaccatcta caaataattg ctgaataaat
    11401 gttctcggat aatacgtccc ttcatcaact tctactaaat gaggatctaa ttgttctttg
    11461 ataagcgttt ctaaagtcat catcatctct ccttattcac caaatcttct aagtataaac
    11521 tgctcaatat aactaatgaa tcgatcaata ataaaaccaa ataatccgat aaagaatatt
    11581 gctgctaaaa catcttctaa gttcaacata tttcgtgcat caacgattaa aaaacctaat
    11641 cccgattgtg caccaatcat ttcaccagaa actaaaaata tccaacttgt tcctaccgcc
    11701 atatgtatcc cagccatgat ttgtttaaat gccccgggaa ataatatatt gcgatacaat
    11761 gaccacccag ttaaatttaa atttgctgct atttttaaat attgaggttc aatgtctcta
    11821 acgcctttaa tagtattgaa cacaattggg aaaaaagcag cgataaaaat aatcgcaatc
    11881 gctggcaaac taccaatacc aaaccataga acaacaaatg gtgcccatgc tatcggagat
    11941 atcggcctaa tcaattgaaa tagcggttcg atagcgttgt atagccaacg attccttcca
    12001 agcaagaagc ccaatggaat agcaaccaac aatgcgacaa caaagcccgc tacaaatctc
    12061 cataaactaa ttgctaaatg ttggaaaatt tctccagtaa caatgaaaga ccatatactt
    12121 tttcctacaa gagcaggacc cggtaacaat acaggttggt aatgcccaat aataatgacc
    12181 atttcccaaa tgcctaagaa aataataaat gtgataatag gtaatataaa tttgttattt
    12241 gtgggacgtg tcatgaacgc gatgcctcct tatacaatga cggttcaaca aagtcatcat
    12301 atgcaggtgg attaaacaaa tgatgttgtt ttaccaatgt cgtaatttct tgatagccgg
    12361 atggcttaat tgttaaatca ccataggatg tccatgccgc tgactgtgtt aaaacgtcac
    12421 gactttgttt aaaatgatgc gtcataatgt ctacactttg cttgcgatca ttcattttaa
    12481 agccagactt tttataatct tgtacaaatg cttgcgctac atccttgtgt tgatcaagca
    12541 attcccctct cagtactagc acacagcaat acgcatcagg tataacgtca tcaccatgtt
    12601 tcaaagtctt acctttgcct aacttttcac ccagtgcacc gaatggttcg gctacagaat
    12661 accctgtaat tctgtgttca ctcaatgcgg ctggcatttc tgctggcgac atttcatgat
    12721 agctaaaatg ccccggttta atctttaatt gtttacgtaa ttcctcaagt aaaagataat
    12781 gtgttgaata acgatgtggt ataccaaaat ggtaatcatc gccattatta ttaaattcat
    12841 ttaagtgcat acctttttgt cccataatga cattgccttc atgatggccc aatgccacag
    12901 cctttatatt tgagcccttc tgttttgatt tcatcgctag ctctattaaa gttgatgcac
    12961 catcaatacg accactgttt aatgcgtcca ttaaatctgg ccaattattg aatttaacta
    13021 attctagttt atatttcgga tgattgtatt gtgataataa ttttttagtc atcatcaaat
    13081 tagctgaatg tgtaatcggc aaatatccaa ttttaatcac ttgctgattt tgggcatttt
    13141 tagaccgttc tttagacgtc ctttgccaat cacatcctgt aattataaag attccaatga
    13201 tgacgattat gcttaacctt ttcatcgtca ctcactcctt ataaataata ttcaggttca
    13261 acttgatgat gattcaatgc aaatgtttcc ataatttcat tacgaatctt aagtaggtgg
    13321 ctatcattac gactgcgtgg atgtgatgct gtaatttcat attgagaaat aatattgcac
    13381 ccttcaccta acagaacaat gcggtcggaa agataaatag cttcatcaat gtcatgcgtc
    13441 actaaaataa tagttgattg cgttttatgt tttagttgca ctagttgatc ctgaagttta
    13501 taacgtgtaa atgcatctaa tgcacctaat ggctcatcca tcgatataac gttaggctta
    13561 tgcacatgcg ctcgacatag tgccacacgt tgtttcatac ccccggacag ttgctcggga
    13621 aaatgctttc ccctgtcttc taaatcaact aatttaagct gtgcgttaat ctcttcatca
    13681 ctaattttct gttgtaatcc aatcctaatg ttgtcattaa tcgttttcca tggcagcaaa
    13741 ttatgatgtt gaaatagcat taaacaatct ggagatggct gttgtttaat ttcgttatca
    13801 ataatgacac gaccagacga tggatgaata aatccaccga taatattgag taaagtagac
    13861 tttccgcaac cacttttccc tatgaaagtg actatttctc ccttgctaat gtccaaatta
    13921 aagttatgaa ttactttatg tgatccaaag tgatgttgta attgttgaat tttaatcatg
    13981 acaagccccc ttttaacccc tactatttta ataggaatta agtgtatatc taaagggcta
    14041 tgccaaacac agcccttata tcgtgaattg ttatatctat agatgattat ggtttggtat
    14101 gagtgctctt ataacataag cgttattcaa ttaacgacgt tatattaatt tcagctctac
    14161 taaatttcaa taaaatgatt gttttagaca atacttgcat ttagttcatt agtttcttca
    14221 tatcaactac ttacatttat agcattgagc tattctaatt aactgcaatc gaaatccatg
    14281 ccaaacctat atacatatat ctcatcaact tgttgtctta ttaaagtttt tcgatgccat
    14341 caatcggtgt atcaactgct ttgcgcgcgc gacgtaccgc ttcttcatca ggtgcgttgt
    14401 ataaacaaat acatttagac atatcttcac atacatatgt gcgtttaaat tcaacttctg
    14461 gcacttcttc ataatgaaca gaatttttct ttttacgtgc taaatattga tccatcgtaa
    14521 tgccttccgg aatgttccaa gttacaaggt aatcaacatg tgcatcacca tttttaactt
    14581 catctaaatc tttaccaact aagcgtactt ctttgactaa ttgaatacta atatctgctt
    14641 ctgttaaaaa ttgtttagct tctgcttcgt catttgcttc cacaataaaa taaccatgag
    14701 ttaaattttc agtagcttgt acttcaatta atgttggaat tgtcttcgta gatagtgatg
    14761 ctgcctttgc ttctagttct tctttcgttg atgcaaaatc aagattgtta gcttctaata
    14821 aaaataatgt cattaataag acctcctata acaatggttg aaaaaatatt actataattc
    14881 ttatcggatt acaaggatat taagatattt ttttaaatat ttagataaaa ttctgtaaaa
    14941 tatattttca aagagtataa aaatcatgtt agggtataag taaataaaca tatatatagg
    15001 gggctatcat tatggataaa gaatctgtcg tagcaagttt agctagaaac aaaaagattg
    15061 ctgtagaaac aatggccggt caaaggtaca tcatagaacg cattt
//
LOCUS       DANRDI010000056        15000 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.056, whole genome shotgun sequence.
ACCESSION   DANRDI010000056 DANRDI010000000
VERSION     DANRDI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15000
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.056"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..261
                     /locus_tag="PZ377_001324"
     CDS             <1..261
                     /locus_tag="PZ377_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDM6358201.1"
                     /translation="NTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASP
                     EHNYQFGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            713..1135
                     /locus_tag="PZ377_001325"
     CDS             713..1135
                     /locus_tag="PZ377_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDM6358202.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(1247..2785)
                     /locus_tag="PZ377_001326"
     CDS             complement(1247..2785)
                     /locus_tag="PZ377_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDM6358203.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            3018..3836
                     /locus_tag="PZ377_001327"
                     /pseudo
     CDS             3018..3836
                     /locus_tag="PZ377_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            4099..4464
                     /locus_tag="PZ377_001328"
     CDS             4099..4464
                     /locus_tag="PZ377_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358204.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            5331..6152
                     /gene="cntK"
                     /locus_tag="PZ377_001329"
     CDS             5331..6152
                     /gene="cntK"
                     /locus_tag="PZ377_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDM6358205.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            6163..6981
                     /gene="cntL"
                     /locus_tag="PZ377_001330"
     CDS             6163..6981
                     /gene="cntL"
                     /locus_tag="PZ377_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDM6358206.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            6974..8275
                     /gene="cntM"
                     /locus_tag="PZ377_001331"
     CDS             6974..8275
                     /gene="cntM"
                     /locus_tag="PZ377_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDM6358207.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            8418..10016
                     /gene="cntA"
                     /locus_tag="PZ377_001332"
     CDS             8418..10016
                     /gene="cntA"
                     /locus_tag="PZ377_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDM6358208.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            10029..10964
                     /locus_tag="PZ377_001333"
     CDS             10029..10964
                     /locus_tag="PZ377_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358209.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            10961..11830
                     /locus_tag="PZ377_001334"
     CDS             10961..11830
                     /locus_tag="PZ377_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358210.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            11827..12642
                     /locus_tag="PZ377_001335"
     CDS             11827..12642
                     /locus_tag="PZ377_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358211.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            12635..13384
                     /locus_tag="PZ377_001336"
     CDS             12635..13384
                     /locus_tag="PZ377_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358212.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            13396..14589
                     /locus_tag="PZ377_001337"
     CDS             13396..14589
                     /locus_tag="PZ377_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6358213.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(14813..>15000)
                     /locus_tag="PZ377_001338"
     CDS             complement(14813..>15000)
                     /locus_tag="PZ377_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358214.1"
                     /translation="IVLSIILETLFYYPYINTWWEFIPALILAGSATYLYLKSYRNFM
                     VSYFYQLAVILVLHIFM"
BASE COUNT         5177 a         2256 c         2910 g         4657 t
ORIGIN      
        1 aatactaaag aaacgaacac gattaaatct gtacatttca caaacttgga aacaagtcct
       61 atgggaagtg ctgtgattga aggatacatc aatgaaaata aagaagatga ttttactgct
      121 tatgcatcgc ctgaacataa ttatcaattt ggtggcgcta tgataaaaag tgaaggagta
      181 gataaattat taaaaccagc acatgaaaga aaatcaccag aaaaaatcaa agaagaatta
      241 gataaaaaag aaggccacta gggtcttctt tatttttgat ttaatcttcc aataatctat
      301 gtcattgcta tcgaaggtgt ttcgcaatta atataaatca cttcatcatc accaatactt
      361 ccccagtttt gtacagtaca ttaacacaaa cgaaccacgt taatttaaat ggaataatag
      421 tttggccatt ataagaacaa tatatatcga ataacaatgt cgatgacgtg atttattgac
      481 attatcaaat tttctcaaat gtgtgtgatt ttttataaaa aagggtagag caaggtataa
      541 tatcctttca atctgttttc atattttata tattttttaa atataagtgc taaatgtttt
      601 aactaaagca tagattgaca agatgttata cagaatttca aattctatcc aatattgttc
      661 gaagtgtagt atcactggat tggtattaaa caatgtaaag gagagattgc aaatgccgta
      721 taattacaag aaacaaaatg gagagttaat gtctgtaatg agccaaggtg aaaagtttat
      781 tcatcaatca cccgttaatg atgaacttag tgcattgatt aagttattaa tttctaaaat
      841 taacggttgt cattattgtg ttgatatcca taaaaaagaa ttaaaggaat tgggtgtaac
      901 acaaatgaaa attgatgaag tcttgagttt tagacattta gatttattta ctgatcaaga
      961 aaaagtgacg cttgaatttg cagaaatgtt aaattcaatc aaagacttta agaagtttga
     1021 aattattgac cggctaaaat cattttatga tgaagaacaa attattgatc ttgtctttgt
     1081 tgtaaaccaa attaacggtt ggaacagatt aaatattatt agtgatagac tataattgtt
     1141 catataaatg cagagtttca tctcgaacgc tatatcataa caaatcatgc cactatacag
     1201 gtcaaatctt gtatagtggc attttaattt atccctttga atactgttat ttaacgaata
     1261 tcggtccacc tggtccaaca gggataccta ataggaacca aatgatgaca aatactgtcc
     1321 atacaatact tagcgcgatt gaatacggca ttaaactaga aagtaaggct ccgagtttca
     1381 tgcgtttatc gtatttttgt gcataagtta ataataaagg taagtacggc atcatcggtg
     1441 taattggatt ggtaattgaa tcgcctacac ggtaaatgac ttgtgtgaat gcgggatgaa
     1501 agccgataag gattaacatt ggtacgaata tcggtcctaa aataccccat ttagccgatg
     1561 cgcttccgat taacatgttg accattgcac tcagtacaat aatacctagt atcaatacaa
     1621 taccgttttg atgttctaat aatttggcac ctttaacagc agcgataatt cctaaattac
     1681 tccactttaa atacgcaagt agctgtgctg caaaaaacac aataacgata aatgttccca
     1741 ttgatcctac agcatcgccg aacattttac ctaagtcttt tgtattttta atttctttgc
     1801 ttaaaatccc ataaactaat ccaggtacta aaaatacgac aagaataatt aatccgacac
     1861 cgttaattaa tggcgcatcg tctagtaagc tgcctgtttt agcatttctt aaaaagctat
     1921 gttcaggaat ggctgtaata attaataaaa taattgtgac tatgaaactg atatttgccc
     1981 attttaaagc atgtgcttct ttgtcagtaa tatgtgaaga tgtttcttca tggtcatcat
     2041 gcattaaact gtcatcatat tttcctaatc taggaataat gagcttagtt gtaactagca
     2101 atatcgtagg aagtaataca acgacactcg ctgcgataaa gtaccagttc atagcaacgt
     2161 ttgttttaat agaatctgaa acgatacgtg ttgccggttc tgtaaatgaa tagaccaaag
     2221 catcttgcat accaacaact atatttgctg caaatcctcc aacagcggaa gcatatgcca
     2281 tcgttagtcc agcgataggg tgatagccaa ttttaataaa aagcattgct gcaagcggcg
     2341 gcaagataat tgtcgcagca tcgccggctg tactacctaa aataccaatt aatataatag
     2401 tcggtaaaat taagaagcgt ggtgcgcgat tcacaacaga aatcattaac ttatcgaagt
     2461 atcctgtttt ctctgcaaca ccaataccaa tcatcactgc tagtactaag cctaatgctg
     2521 ggaactctga gaaattttta atcgtatcat tcattatcat cgtaaatcca tcatggctaa
     2581 ttatattttt aatataaatg gtttgatgcg tacctggatg cttaacagat acattaaata
     2641 atgagataac ccatgtcata atggctaagc ccacacacat taaaaagaat aagacgctag
     2701 gatctggcaa tttatttccg attttttcaa cactattcaa gaaacgattg acgatagacc
     2761 ccttttgttg atgttttgat gtcatcaatt attcccccct ttgttaaata tttaaagtgt
     2821 aacaaaaaat actctcaaaa gtaacaattt tcagaaaata aaaaaactaa tattgttaaa
     2881 tattttgagt tattcaatag aaagtgtata gcagagtagt taagactgcc tgaagactta
     2941 tctattaggt ttatgaagca tcgaacagtg gaaaataagg actgtaagtt taagatatgt
     3001 tgtataggag tgactgaatg aaacgtttgg aaaataaagt agcagtcgta acaggagcaa
     3061 gtacaggtat cggtcaagct tctgcaatcg ctttagctca ataaggtgcg tatgtattgg
     3121 cggtagacat agctgaagcg gtatcggaga ctgtcgataa aattaaaagt aatggtgaca
     3181 atgcgaaggc gtataatgtg gataatgcaa gcgaacaaca agtggtagac tttgtgtctg
     3241 acataaagga acagtttgga agaatcgatg tgttgtttaa taatgccggt gtggataatg
     3301 cggctgatag aattcatgag tatccaatag atgtgtatga caagattatg aatgtagata
     3361 tgcgtgggac atttttaatg acgaaaatga tgttaccttt aatgatgaat caaggtggct
     3421 ctattgttaa tacgtcttca ttttccggac aagcagcaga cttgtatcgc tctggatata
     3481 atgctgcgaa aggtgcagtg attaatttta caaaatcaat cgcaattgag tatggccgtg
     3541 atagcattcg agccaatgcg attgcaccag gtacaattga aaccccgtta gtagataaac
     3601 tgacaggtac gagtgaggat gatgcaggta aaacatttag agaaaatcaa aaatggatga
     3661 ctccgctggg acgtttaggt aaaccagaag aagttgctaa attagtagtc ttcttagcat
     3721 ctgacgacag ttcattcata actggagaga cgattcgaat tgatggtggt gtgatggctt
     3781 acacatggcc tggcgagatg ttaagtgatg attcgtggaa gcggacattg gaataattat
     3841 ttaaaatcga tatccagtgg aaaagaattt ggcatgaagt aggcaatgta tgcatattgt
     3901 cgcaatgaaa gacatttaaa gcggagatta attaatcttt gaaaatcaca tattgttcat
     3961 ttgaagtgta ttgcttaaag cttgttttaa cgtattgtag gtcgtgcatc taatttatca
     4021 gaatctcgag ttcaaacttt tggagtatct ttaaaataag ctgtatttta tctattctaa
     4081 taaattaagg agaattttat gttaaaagaa aaagaaagtt ttagattgct atatcaagct
     4141 ataagagaga ttgcagataa aattggagat aatcagttag aaactaattc cgttagttta
     4201 ttattattgg actttgattt tgaacatgaa gtatttgatg aattgtatct tgtgatttta
     4261 aaatatttaa atacagtaag tatagagaac ataagtcata gtgagctttt gaatttaata
     4321 gaaaatacga ttccagaaga tcgagagatt aacacgtttg taaaaaataa aataataatt
     4381 ggctttgcta ataattactt ccctgaatta caagtattag caaacgaaat aaaatctgat
     4441 atggctagtt cattaaaaca atgatatttt tatttaaatt tttaaagctt tgtacgaaat
     4501 tgtacaaagc ttttttggtg cgtattgtat gggcaacaac ttgacgatga aaatccgtta
     4561 caggattggt aataggaaat gttagcgaaa gacaagggta tccattgtag attaacaaaa
     4621 ggacgtttcc acaagtgtgg gttattctca ctaaagcaat acgcagagac aacttacgta
     4681 aaattttgaa ctgactagaa cggaacttct actcaattat tgataaaaat tttcaaaaag
     4741 acttgaatgt gctgagaata cgaagtttat ggaaggatta tcaaaatata aatgtgcatt
     4801 catttacaac ctttattgac aatgattctc aactaatata gtatataatc aaatcgtaat
     4861 agttacgatt tgttttctgc aacttttttg aagttttagt tgaggtgaaa acagtaaaag
     4921 catctaagtg aatgtagtta acggacaact gcattcgctt gtagagccac aagaagcaac
     4981 tttaaataag gtttacggtt gcattttgat acaacaaccg attactaagt catgctttcc
     5041 actttgcggg ttagcatgac ttacctaata gatagagcta ttaggttcag cttctaaaaa
     5101 attacagttt tagaggaata cagttgcttg cttcgcaaca actgcataag agccatggtt
     5161 ttcgcttttg cgaattagca tgacttacct actagataga gctattaggt tcatcttcta
     5221 aaaaattaca ggtttagagg aatacagttg tttgcttcgc aacaactgca taagagcctc
     5281 tagtaattaa aattacagag gctctaaaaa tacatctaaa ggagtgtcgt atgaatcggc
     5341 aggttataga attttctaag tataatcctt cggggaatat gacgatactt gttcattcaa
     5401 aacatgatgc tagtgaatat gcatctatcg ccaatcagtt gatggccgca acacatgtat
     5461 gctgtgaaca ggtaggcttt atagaatcaa cacaaaatga tgatggtaat gattttcact
     5521 tagttatgag cggtaatgaa ttttgcggta atgcgacgat gtcatatata catcatttgc
     5581 aggaaagtca tttgcttaaa gaccaacagt ttaaggtgaa ggtgtctggc tgttcggatt
     5641 tagtgcaatg cgcaattcat gattgccaat actatgaagt tcaaatgcca caagcccatc
     5701 gtgttgtgcc aacaacaatt aatatgggta atcattcatg gaaagcaata gaaattattt
     5761 atgaaacata tgtacattat gtgattccag ttaaacaagt aacaacagaa attcaacatt
     5821 tggttgaagc gtttgtgcgt gagcaacaat ggagtcacaa atataaaaca gtaggtatga
     5881 tgctttttga tgaacaacgt caatttttac agccattaat ctatatacca gaaattcaaa
     5941 gtttaatttg ggaaaatagc tgtggttctg gtacagcatc aattggggtt tttaataatt
     6001 atcaacgtaa tgacgcatgc aaagatttta cagtacatca gccagggggc agtattttag
     6061 tgacatcaaa gcgatgtcat caattgggat atcaaacttc aattaaagga caggttacaa
     6121 ctgtagctac aggaaaagca tatatagaat aaggagccta caatgaataa ctttaataat
     6181 gaaatcaaat tgatattaca acaatattta gaaaagtttg aagcgcatta cgagcgtgta
     6241 ttacaagacg atcaatatat cgaagcatta gaaacattga tggatgacta tagtgaattt
     6301 attttaaatc ctatttatga acaacaattt aatgcttggc gtgacgttga agaaaaagca
     6361 caattaataa aatcactgca atatattaca gcgcagtgtg ttaaacaagt ggaagtcatt
     6421 agagcgagac gtctattaga cggacaggcg tctaccacag gttactttga caatatagaa
     6481 cattgtattg atgaagagtt tggacaatgt agtatagcta gcaatgacaa attattgtta
     6541 gttggttcag gtgcatatcc aatgacgtta attcaagtag caaaagaaac aggtgcttca
     6601 gttatcggta ttgatattga tccacaagcc gttgacctag ggcgcagaat cgttaacgtc
     6661 ttagcaccaa atgaagatat aacaattacg gatcaaaagg tatctgaact taaagatatc
     6721 aaagatgtga cgcatatcat attcagctcg acaattcctt taaagtacag cattttagaa
     6781 gaattatatg atttaacaaa tgaaaatgtc gtagttgcaa tgcgctttgg tgatggcatc
     6841 aaagcaatat ttaattatcc gtcacaagaa acagcggaag ataagtggca atgtgtgaat
     6901 aaacatatga gaccacagca aatttttgat atagcacttt ataaaaaagc agctataaag
     6961 gtaggtatta cggatgtcta aattattaat gataggcact ggtccggtcg caatccaatt
     7021 agcgaatatt tgctatttaa aatcagatta tgagattgat atggttggac gtgcctcaac
     7081 atcagaaaaa tcaaaacgct tatatcaagc gtataaaaaa gcgaaacaat ttgaagtcaa
     7141 aatacaaaac gaggcgcatc aacatctgga aggtaagttt gaaattaatc gtttgtataa
     7201 agatgttaaa aacgttaagg gtgaatacga aacggttgtc atggcatgca cagcagatgc
     7261 ttattatgac acactacagc aattgtcgtt agaaactttg caaagtgtca aacatgtcat
     7321 tttaatatca ccgacatttg gttcgcaaat gattgtcgaa caatttatgt ctaaatttaa
     7381 taaagatatc gaagtgattt cattctcaac ttatcttggc gatacacgta ttgttgataa
     7441 agaagcgcct aatcatgtgt tgacaacagg tgtaaaaaag aaattgtaca tgggatcgac
     7501 acattcaaac tcaacaatgt gtcaacgaat ctctgcttta gctgagcaat tgaagattca
     7561 attagaagtc gttgaatcgc cactgcatgc tgaaacgcga aacagttcgc tttatgtgca
     7621 cccaccacta tttatgaatg acttttcatt gaaagccatt ttcgaaggaa cagatgtacc
     7681 ggtttatgtg tataagttat ttcctgaagg accgataacg atgacactaa tccgtgaaat
     7741 gcgtttaatg tggaaggaaa tgatggttat tttacaagca tttagagtgc cgtcagtcaa
     7801 cctgcttcaa tttatggtga aggaaaatta tccagtacgt cctgaaactt tggatgaagg
     7861 tgatattgag catttcgaaa tcttgccaga tatcttacaa gaatatctgc tttatgtaag
     7921 atataccgca atcctcattg atccattttc acagccagac gaaaacggac attactttga
     7981 tttttcagct gtaccattta agcaagtcta taaaaatgaa caggatgttg ttcaaattcc
     8041 aagaatgcca agtgaagatt attacagaac ggcgatgatt cagcatattg ggaaaatgct
     8101 aggtatcaaa acgccaatga ttgatcagtt cctaactcgc tatgaagcaa gttgccaggc
     8161 gtacaaggat atgcatcaag atcaacactt atcttctcaa tttaatacaa atctatttga
     8221 aggagataaa gcactcgtca caaaattttt ggaaatcaat agaacgcttt cataataagg
     8281 gtttgaagtt ttataataga aaaaaattat tgaattatgt ttgacattta cataaaaata
     8341 agcaaataat tgagaaaaat aatcattacg atttgattaa gtaatgcaac ttatcaattt
     8401 agaaagagga aaagcaaatg agaaaactaa ctaaaatgag tgcaatgtta cttgcatcag
     8461 ggctaatttt aactggttgt ggcggtaata aaggtttaga ggagaaaaaa gaaaacaagc
     8521 aattaacgta tacgacggtt aaagatatcg gtgatatgaa tccgcatgtt tacggtggat
     8581 caatgtctgc tgaaagtatg atatacgagc cgcttgtacg taacacgaaa gatggtatta
     8641 agcctttact agctaaaaag tgggatgtgt ctgaagatgg gaagacatac acgttccatt
     8701 tgagagatga cgttaaattc catgatggta cgccatttga tgctgacgca gttaagaaaa
     8761 atattgacgc agttcaagaa aacaaaaaat tgcattcttg gttaaagatt tcgacattaa
     8821 ttgacaatgt taaagttaaa gataagtaca cggttgaatt gaatttgaaa gaagcatatc
     8881 aacctgcatt ggctgaatta gcgatgcctc gtccatatgt atttgtgtct ccaaaagact
     8941 ttaaaaacgg tacaacaaaa gatggcgtta aaaagttcga tggtactggt ccatttaaat
     9001 taggtgaaca caaaaaagat gagtctgcag actttaacaa aaatgatcaa tactggggcg
     9061 aaaagtctaa acttaacaaa gtacaagcaa aagtaatgcc tgctggtgaa acagcattcc
     9121 tatcaatgaa aaaaggtgaa acgaactttg ccttcacaga tgatagaggt acagatagct
     9181 tagacaaaga ctctttaaaa caattgaaag atacaggtga ctatcaagtt aagcgtagtc
     9241 aacctatgaa tacgaaaatg ttagttgtca attctggtaa aaaagataac gctgtgagtg
     9301 acaaaacagt cagacaagcg attggtcata tggtaaacag agataaaatt gccaaagaaa
     9361 ttttagatgg tcaagaaaaa ccagcaactc aattatttgc gaaaaatgta acagacatta
     9421 atttcgatat gccaacacgt aagtatgacc ttaaaaaagc agaatcatta ttagatgaag
     9481 ctggttggaa gaaaggtaaa gacagcgatg ttcgtcaaaa agatggtaaa aaccttgaaa
     9541 tggcaatgta ctatgacaaa ggttcttcaa gtcaaaaaga acaagcagaa tacttacaag
     9601 cagaatttaa gaaaatgggt attaagttaa acatcaatgg cgaaacatca gataaaattg
     9661 ctgaacgtcg tacttctggt gattatgact taatgttcaa ccaaacttgg ggattattgt
     9721 acgatccaca aagtactatt gcagcattta aagcgaaaaa tggttatgaa agtgcaacat
     9781 caggcattga gaacaaagat aaaatataca acagcattga tgacgcattt aaaatccaaa
     9841 acggtaaaga gcgttcagac gcttataaaa acattttgaa acaaattgat gatgaaggta
     9901 tctttatccc tatttcacac ggtagtatga cagttgttgc accaaaagat ttagaaaaag
     9961 tatcattcac acaatcacag tatgaattac cattcaatga aatgcagtat aaataaagga
    10021 gcaattagat gttcaaattt atcttaaaac gtattgcgct catgtttcca ttgatgattg
    10081 tagtaagttt tatgacattt ctattgacgt atattacaaa tgaaaatcca gctgtgacaa
    10141 ttttacatgc acaagggacg ccaaatgtaa caccagagtt gattgcagaa acgaatgaga
    10201 agtacggttt caatgatcca ttattaattc aatataaaaa ttggttactt gaagcgatgc
    10261 aatttaattt tggtacaagc tacattacag gtgacccagt tgctgaacgt attggtccag
    10321 catttatgaa tacattgaaa ttaacaataa tttcaagtgt tatggtgatg attacatcaa
    10381 ttattttagg tgtagttagt gcattaaaaa gaggaaagtt cactgatcgt gcgatacgtt
    10441 cagtggcttt ctttctaact gcattaccat catattggat agcttcaata cttattattt
    10501 acgtttcagt gaagttaaac atattgccga cttctggatt aacaggtcca gaaagttaca
    10561 tattgccagt gatcgttatt acgattgcct atgctggtat ttactttaga aatgttagac
    10621 gctcgatggt ggaacaatta aatgaagatt atgtacttta tttaagagca agcggtgtga
    10681 aatctatcac attaatgttg catgtgttgc gtaatgcttt acaagttgcg gtatcaatct
    10741 tttgtatgtc tataccaatg ataatgggtg gactagttgt tatcgagtat atctttgcat
    10801 ggcctggact aggtcaatta agtttaaaag caatacttga acacgatttt ccagtcattc
    10861 aagcatatgt attaattgta gcggtattat ttattgtatt taatacatta gcagatatca
    10921 ttaatgcgct attaaatcca agattaaggg agggcgcacg atgataattt taaaacgatt
    10981 attacaagat aaaggtgcag taattgcttt aggcattatt gtattatatg tctttttagg
    11041 attagcagca ccacttgtga cattttatga tcctaaccat atcgatacag caaacaaatt
    11101 tgctggcatg agttttcaac atctactagg tactgaccat ttaggtagag atattttaac
    11161 taggttaatt tatgcgatta gaccaagttt gttatatgtc tttgttgcgc tatttgtttc
    11221 tgtacttatt ggatctattt taggattctt atcaggatat ttccaagggt ttgttgacgc
    11281 cttaatcatg cgtgcgtgtg atgttatgtt ggcattccca agttatgttg taacgttagc
    11341 attaattgca ttgtttggaa tgggtgccga aaatattatc atggcattta ttttgacgcg
    11401 ttgggcatgg ttctgtcgtg ttatacgtac aagtgttatg cagtacactg cttctgacca
    11461 tgtaagattt gctaaaacaa tcggtatgaa tgatatgaaa attattcaca aacatattat
    11521 gccattaaca ttagcagata ttgctatcat ctctagtagc tcgatgtgtt caatgatctt
    11581 gcaaatatct ggcttttcat ttttaggatt aggtgtcaaa gcgcctactg cagagtgggg
    11641 catgatgctt aacgaagcta gaaaagtgat gtttacacat cctgaaatga tgtttgcgcc
    11701 aggtattgcc atagtgatta tagtgatggc atttaacttc ttatccgatg ctttacaaat
    11761 tgctattgat ccccgcatct cttctaaaga taaacttcgt tctgtgaaaa aaggagtggt
    11821 gcaatcatga cattgttaac agttaaacat ttgacgatta cagatacctg gacagatcaa
    11881 ccactcgtga gtgatgtgaa ttttacatta actaagggtg aaactttagg cgttattgga
    11941 gaaagtggta gtggtaaatc aatcacttgt aaatcgatta ttggtttgaa tcccgaacga
    12001 ctcggggtga caggtgaaat tatctttgat ggtacatcaa tgttgtcatt atctgaatcg
    12061 caattgaaaa agtaccgtgg taaagacatt gcgatggtca tgcaacaagg tagtcgtgcc
    12121 tttgacccat caactactgt cggtaaacaa atgtttgaga ctatgaaagt acatacgtca
    12181 atgtctacac aagaaattga aaagacattg attgaatata tggattattt aagtttgaaa
    12241 gatcctaaac gtatattaaa atcataccct tacatgttat caggaggaat gttacagcga
    12301 ttgatgattg ctttagcgtt agctttgaaa ccaaagttaa tcattgctga tgagccgaca
    12361 acggctttag atacaattac acaatatgat gtactggaag catttataga tattaaaaaa
    12421 cactttgact gtgcgatgat tttcatttca catgatttaa cggttattaa caagattgca
    12481 gaccgtgttg ttgtgatgaa aaatggtcag cttattgaac aagggacacg tgaatcagtc
    12541 ttgcatcatc cagaacatgt ttatacgaag tatttattat caacgaagaa gaagattaat
    12601 gatcatttta aacatgtgat gaggggtgat gtacatgatt aaaattaaag atgttgaaaa
    12661 gtcatatcaa agcgcacatg tttttaagcg tcgtcgaaca cctatcgtga aaggtgtgtc
    12721 atttgagtgt ccaatcggtg cgacgattgc gattatcgga gaaagtggta gcggtaaatc
    12781 gacgttgagt cgtatgatat taggtattga gaaaccggat aaaggttgtg taaccttaaa
    12841 tgatcaaccg atgcataaga agaaagtgag acgtcatcaa attggtgctg tatttcaaga
    12901 ttatacgtca tcattacatc catttcagac tgttagagaa atcttatttg aagtgatgtg
    12961 tcaatgtgat ggacaaccta aagaagttat ggaagtccaa gcaattacat tgttggaaga
    13021 agtcggtcta tctaaggcat acatggataa atatcctaat atgttatcag gtggagaggc
    13081 gcaacgtgtt gcgattgcgc gtgcaatatg tattaaccct aaatatattt tgtttgatga
    13141 agccattagt tcactcgaca tgtcaattca aacacaaata ttagatttat tgattcattt
    13201 acgtgaaacg cgtcagttga gttatatttt tatcacacat gatattcaag ctgccacgta
    13261 tttatgtgat caattaatta tttttaaaaa cggaaaaata gaagaacaaa ttccgacaag
    13321 cgcattgcat aaaagtgaca atgcttatac aagagaatta atagaaaaac aactatcatt
    13381 ctaaggagtg agataatgaa aggtgcaatg gcttggccct ttttgagatt atatatatta
    13441 acattgatgt tctttagtgc caatgcaatc ttaaacgtgt ttataccttt acgagggcat
    13501 gatttaggcg caacgaatac ggttatcggt atcgttatgg gggcatacat gttaacagca
    13561 atggtatttc gaccatgggc aggacaaatt attgctcgtg tcggtcccat taaagtatta
    13621 agaattattt tgattatcaa tgccatagct ttaattattt atggttttac tggcttagaa
    13681 ggttatttcg tagcacgtgt tatgcaaggt gtgtgtacgg cattcttttc tatgtcttta
    13741 cagctaggta ttattgatgc attaccagag gaacatcgtt ctgaaggtgt atcattgtac
    13801 tcgctatttt caacgattcc aaacttaatc ggaccattag ttgccgtagg tatttggaat
    13861 gcaaataata tttcactatt tgcaattgtc attatcttta tcgcattaac aacaacattc
    13921 tttggctatc gcgtgacctt tgctgaacag gaacccgata cgtcagataa gattgaaaaa
    13981 atgccgttta acgctgtaac tgtttttgcg caatttttca aaaataaaga gttgttgaac
    14041 agtggtatta tcatgattgt tgcatcgatt gtatttggtg cagttagtac atttgtaccg
    14101 ttatacacag tgagtttagg attcgcgaat gcgggaatct ttttgacaat acaggccatc
    14161 gcagttgttg cggcaagatt ttacttaagg aaatacattc cgtcagatgg tatgtggcat
    14221 cctaaatata tggtatctgt actatcatta ttagtaatcg cgtcatttgt agtggcattt
    14281 ggtccgcaag taggtgcaat tattttctat ggtagtgcga tattaatagg aatgacgcaa
    14341 gcaatggtgt acccaacatt aacatcatac ttaagcttcg tcttaccaaa agtaggtcgt
    14401 aatatgttgt taggtttatt tattgcctgt gcagacttag gtatatcgtt aggtggcgca
    14461 ttgatgggac ctatttccga tttagtagga tttaaatgga tgtatctaat ttgtggtatg
    14521 ttagtcattg taataatgat tatgagtttc ttgaaaaagc caacaccacg tccagcgagt
    14581 agtctttaat gaagtgaatt aaagcatatt aagttaatga atatttaaat tttaaaaggt
    14641 atattgagca tggcgattca tgtgcttcat gctaggacat gaaacattct atatggctcg
    14701 tttttagaac gacagtatat ctaaataaag cacgcttaaa agtgagtttt gatactgact
    14761 tttaagtgtg ctttttgatg tgtatggata atggataata ctctgaaaaa ttttacatga
    14821 aaatatgcaa aacgagtata actgctaatt gatagaaata gctcaccata aaattacggt
    14881 atgattttaa atataagtaa gtcgcactac ctgctagtat caatgctgga atgaattccc
    14941 accatgtatt aatgtatgga tagtagaaca gagtttcaag gataatggac aatactattg
//
LOCUS       DANRDI010000057        14929 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.057, whole genome shotgun sequence.
ACCESSION   DANRDI010000057 DANRDI010000000
VERSION     DANRDI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.057"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(67..945)
                     /locus_tag="PZ377_001339"
     CDS             complement(67..945)
                     /locus_tag="PZ377_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDM6358215.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(1124..1504)
                     /gene="gcvH"
                     /locus_tag="PZ377_001340"
     CDS             complement(1124..1504)
                     /gene="gcvH"
                     /locus_tag="PZ377_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDM6358216.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            complement(1662..2018)
                     /locus_tag="PZ377_001341"
     CDS             complement(1662..2018)
                     /locus_tag="PZ377_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDM6358217.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            2162..2482
                     /locus_tag="PZ377_001342"
     CDS             2162..2482
                     /locus_tag="PZ377_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDM6358218.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            complement(2632..3171)
                     /locus_tag="PZ377_001343"
     CDS             complement(2632..3171)
                     /locus_tag="PZ377_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDM6358219.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(3254..3970)
                     /locus_tag="PZ377_001344"
     CDS             complement(3254..3970)
                     /locus_tag="PZ377_001344"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDM6358220.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            4118..4540
                     /locus_tag="PZ377_001345"
     CDS             4118..4540
                     /locus_tag="PZ377_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDM6358221.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            4939..5433
                     /locus_tag="PZ377_001346"
     CDS             4939..5433
                     /locus_tag="PZ377_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6358222.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            5588..6205
                     /locus_tag="PZ377_001347"
     CDS             5588..6205
                     /locus_tag="PZ377_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDM6358223.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(6278..6859)
                     /locus_tag="PZ377_001348"
     CDS             complement(6278..6859)
                     /locus_tag="PZ377_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDM6358224.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            7084..7320
                     /locus_tag="PZ377_001349"
     CDS             7084..7320
                     /locus_tag="PZ377_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358225.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            complement(7317..7520)
                     /locus_tag="PZ377_001350"
     CDS             complement(7317..7520)
                     /locus_tag="PZ377_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDM6358226.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            7861..8121
                     /locus_tag="PZ377_001351"
     CDS             7861..8121
                     /locus_tag="PZ377_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358227.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            8522..9091
                     /locus_tag="PZ377_001352"
     CDS             8522..9091
                     /locus_tag="PZ377_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358228.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            9179..9463
                     /locus_tag="PZ377_001353"
     CDS             9179..9463
                     /locus_tag="PZ377_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358229.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            9525..9743
                     /locus_tag="PZ377_001354"
     CDS             9525..9743
                     /locus_tag="PZ377_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358230.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(10239..10439)
                     /locus_tag="PZ377_001355"
     CDS             complement(10239..10439)
                     /locus_tag="PZ377_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDM6358231.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(10796..11482)
                     /locus_tag="PZ377_001356"
     CDS             complement(10796..11482)
                     /locus_tag="PZ377_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDM6358232.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
     gene            complement(11823..12293)
                     /locus_tag="PZ377_001357"
     CDS             complement(11823..12293)
                     /locus_tag="PZ377_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358233.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
     gene            complement(12628..13650)
                     /gene="emp"
                     /locus_tag="PZ377_001358"
     CDS             complement(12628..13650)
                     /gene="emp"
                     /locus_tag="PZ377_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDM6358234.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            complement(14001..>14929)
                     /locus_tag="PZ377_001359"
     CDS             complement(14001..>14929)
                     /locus_tag="PZ377_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDM6358235.1"
                     /translation="MGYKDEERANKKAVNKRMLENKKEDLETIIDEFFSDIDKTRPNN
                     IPVLEDEKQEEKNHKNMAQLKSDTEAAKSDESKRSKRSKRSLNTQNHKPASQEVSEQQ
                     KAEYDKRAEERKARFLDNQKIKKTPVVSLEYDFEHKQRIDNENDKKLVVSAPTKKPTS
                     PTTYTETTTQVPMPTVERQTQQQIIYNAPKQLAGLNGESHDFTTTHQSPTTSNHTHNN
                     VVEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSY
                     KDRINAQQKVNTLSEGHQKRFNKQINKVYNGK"
BASE COUNT         4829 a         2407 c         2079 g         5614 t
ORIGIN      
        1 tgtcccactc cctagattga tctatagata ctacacttat taaagtaata tatttttatg
       61 attctcttag ctgcaatccc atgaatacat gtaatcatca aacttcatag cctcaaggtc
      121 agtagatttc actaagatat tggcaatacc taaatcaccc catataatcg aagaattacc
      181 atctcttgta gaatcaattt gcagtaagac tctatcatac attttcagtt cttgatcgtt
      241 cgttcttgga tctatttggt caaaatatgg ataaccacca agttgattaa atggttcctt
      301 agcagtaaat gtgttacaca caggttccat caattcatca taaccaccaa acattgattt
      361 tagcgcgaag gatggattga ctttattcca caatgcaagc acgtcctcat cgtacttata
      421 atcagtacat gtaatagttt gtttcgacaa tctaaattct atagaaaatg ctccactgaa
      481 cgggatatcc tcattatcag catctaatga cttcaaatgc gcttgaatat tttcgagtga
      541 taaatctgta attggctctt ttatataaac aagccttgag tttatattgt ttccctttaa
      601 attttcagac attccataca tttgatcttc tgaactaatc caaaactgca atatcccttc
      661 ttttacagga aataattctt gttgttctgg aagatcatcc aaattaatct gtgctagcaa
      721 taccattggt acattatttt catctaatgg tatatcttta aaatatttta aaaatggcat
      781 tccaccaatc ttactctggt ttaatgtttc ttcttgcatc tcttttagat ccatttcaat
      841 acatggtagt actggtaaat taccgttttc tacagattct acgaaacggt tatatgcttt
      901 ttgctccttt ttagaaagat gactaacgtc ttgaatgttt agcatattaa cttcccccaa
      961 tttttatttt taaaatagct aaattcatta tttccatcat tcttgaataa taaaatgtag
     1021 caatactcca aataatagta cctaataatt tttctttcat atcatttatt ttctcagact
     1081 aaaaataaac tcctcaatct tagattaagg agttacacgg tgattattca ccaatcattt
     1141 ctgaatattt ttcagctgtt aataaagctt caatctgact ttcatcacta atttctactt
     1201 ttaccatcca tgctttttcg tatggagatt catttacaaa ttcgggacta tcttctagtt
     1261 cttcgttgac ttcaactact ttaccagaga ttggtgcata taattctgat acagttttaa
     1321 ctgattctac gctaccaaac gtatcccctt cattaatttc atcatctgtt tctggtaatt
     1381 caacgaaaac aatatcacct aactcgcttt gtgcgtattc tgtgattcca attattgcta
     1441 cattaccttc aactttaacc cattcatgct cttttgaata tttcaattca tttggtactg
     1501 ccaagagaat cccctcctaa ttaatcttta catcattaat gatgccataa ggctagatgc
     1561 tattcaacta tatttataaa aattaacaat tgtatagctt atttcatttt acatgtaatg
     1621 aatctaaata tcgagatatt tagtaaacgc ttacatttca cttacgctaa ccaagtctct
     1681 ttatattgat cttctttaaa tcctaatgtt atcttatcgc ccattactgc tagaggacgc
     1741 tttactaaca taccatcaga tgataacaac tctaactttt catcatctga taaagtttgt
     1801 aatttatttt tcaaatcaag ctcacgatat ttcgcgccgt gtgtattaaa caatttatta
     1861 atttctacgc ctgtatttgc aattattgtt ttaaattcat ttattgtagg tgtatgttga
     1921 acgatatcaa ttggttcata acttacgcca tattcatcta aaaactttgc tgccttttta
     1981 caagttgtac aattcttata ttggtaaaat ttaatcatac gactgcaacc tcctttatgt
     2041 atttaaaata aatatatcat tttccaaagc aaaaggcttt cattttgaag ctaaaatttt
     2101 ttattatgat ataaatttag ctataataaa aattattaaa ctatctagag gagttgttac
     2161 aatgcaatca atcaaaagta atgaatcatt taaatctgta attaatagcg atacacctgt
     2221 aattgttaaa tttgaggcag gatggtgccc agactgtcgt gctatggatt tatggattga
     2281 cccaatcgta gaacaatata atgattacca atggtatact gttaatcgtg atgaattaga
     2341 agatgtagtt gttgaaaatg aagttatggg tatccctagc ttgctagtat ttaaaaacgg
     2401 cgataaaatt gcacaccttc attcagcaaa tgctaagtca cctgaacaag ttgaatcatt
     2461 tttagcagaa acttttaaat aaatactaaa ataaaaatgt gtatatactg cacaataaaa
     2521 agctgagact gttattgtac cgactctagt aagttagatt aaatctctaa ctcgatagat
     2581 gtccatcaat tcatgactca gcttttatta atgtttctat aattatacat attaatataa
     2641 tgtaattaag tttctatttt tacgtggtgc tttaggcata tctccttcta aatccgttaa
     2701 atataagaat ccagcaagaa cttcatcttt ctttatacca agtgtttttc ttactttagg
     2761 atcatagata tataatggcg atttccaaca tgtacctatt cccgcttcat aaagtaacaa
     2821 cataagattt tgtgcatatg caccaaatgc aaagtagttt tcatcatttt gacgttgtct
     2881 tggatctgtt tttaaaataa gcaataacat gccacctagt ttcgtaactg catcataatg
     2941 acattgttgc ttatctaatt cattaggaaa tgcaaattta gaaatatcct tactcatatc
     3001 tcctaatctg tctttcggaa catgcacaac tctccatggt tccctcattc cgtgatttgg
     3061 agcatcagca gctttctcaa ttgcttgata tagcaatgcg tcatctatgt gcatatctct
     3121 tttaaatttt ttcacacttc ttctattagc tattgcttgt tgtaattcca tctaaaatcg
     3181 ctcctccacg aacaatgata atacttctca attataagac agcacttaca caaaagcaac
     3241 ctaattcatt ttattagtat aaagtcactt gtgcttttaa atcagtaaca tcaatctgtc
     3301 ctggagcttg tggtactccg atacaaccat aagtcaacgc accaccaaaa acgccttgag
     3361 ccgttctact tattagtcca agtttagaca ttgaaatacc aacaactttg cagtccatag
     3421 tatctgaaaa tgtagacatt gcctgcaata aatttaacac atcattttta ttatgtggca
     3481 ttactgctaa tttaacgtat tctgggttga atttttgcat tttaaaaaat ataaattgca
     3541 attcatctaa tggaggcgta ctttcgaaat tatgatgtga tataaccacc tctttattat
     3601 actgttgcaa atgtgtaatg attcgttgat gtttttcaat gtcaatatct gcttgccatt
     3661 ctatatcaat catatctatg ccattgatat ttgctaagtc tgatattaaa ttaagatacg
     3721 agtcatttgt aaattgccca tagccacctt gtaactttgt acgatacgta actaataatt
     3781 tgaatgaatc ttgcataacc ttcagctttg taatcatttc tgccacttga tcaactgtga
     3841 cattttcaat ttgatcaatt cgtaattcta atacgtctat tgcatcaata cgatgattaa
     3901 ttttttgaat taaagtttct tcgatagata attgtggcgc gatagtcgct actacttcca
     3961 catgtgtcat agtgaatccc tcatttcaga tactcattat ttttattgta aacaacaact
     4021 ttttatcctt caatcaaaca atgctataaa tgaaaatgat taaggtattt tcgaatgggt
     4081 aaagcataaa tgtattttaa attaggaggt tataagtatg gcaatacatt atgaaactaa
     4141 agcgacgaat gttggcggac gtaaaggaca tgtttatact gatgatcgag cattagatat
     4201 tgatatcgtt ccgcctgctc aagcagatgg taaagctact aaccccgaac aattatttgc
     4261 agcaggttat gcatcttgct tcaacggtgc tttcgaccta attttaaagc aaaacaaagt
     4321 gcgtgatgct catccagaag taacactaac agtgagacta gaagatgatt cagactcaga
     4381 aagtcctaaa ttaagtgttt caattgatgc gacaattaaa aatgttatat ctcaagaaga
     4441 agctgaaaaa tatttacaaa tggctcatga attttgtcca tattcaaaag cgactcaagg
     4501 aaatattaat gtcgatttaa atgtaaatgt tgtagattag cattaactta aagagattat
     4561 tcaacgttat taataaaatt cacataaaat tcaaatttgc caaccaaaaa tttttggttg
     4621 gttatttttt ctattcgtga ttgaaatttc tggcaatatt taactgaaaa tgattgtacc
     4681 ttagtcatca taaatgtgac cggttccaat actggcttga cttcttcgca taccgtctac
     4741 aaataaaagt ccagtgtcga ccaataacca attccataat ttcctgaact taaaaataaa
     4801 tataattaaa ttgataatcc atttacaaga atgtgcatat aaatcagtga ttgcaaaatt
     4861 ttaataaaca ttttaatatg taattaagaa atttttttag tacaaaatct ttcttaataa
     4921 tgattaagga ggtcagtaat gattgaaatt aaaacattaa cgaataatga ttttaatgag
     4981 tataagagac ttgtttcgac agtcaatgaa gaattcactc aagattcaca ttatagtcaa
     5041 acaatgactg acaccttaat acatgacatt ttaaatcaag gttcaccgaa atgtattgta
     5101 tttggctgtt atgaaaacga aacacttatc gcaacagctg ccttagaaca aattcgatac
     5161 gttggaaaag aacataaatc attaattaaa tacaactttg ttactaataa cgataaatcg
     5221 attaatagcg agctcattaa tttcattatt aattatgcac ggcagaacaa ttacgaatct
     5281 ttacttacat caattgtgtc aaacaacata ggtgctaaag ttttctatag tgcactagga
     5341 ttcgacattc ttggttttga gaaaaatgca attaaaatcg gaaataccta tttcgatgaa
     5401 cattggcttt tttatgattt gattaataag taatacagtt ttatatattc tacatttctc
     5461 ataaattcaa atcatatagg ttctattttt catacaacta ctctactatt gagtagttgt
     5521 tttttatttg atatgataac aataagtttt ttcagaatat tctatgtttt aggggtgtca
     5581 aaacttaatg gtaaccgcaa ttattcatgg atttatctta gcgattggtc taattttacc
     5641 tttaggtgca caaaatgtat ttatttttaa ccaaggagct aatcaaccaa aatatagata
     5701 tgtattgcct gcaataatta cagccgggtt gtcagacagc ttacttatta ttattgcagt
     5761 ggtaggagta tctatcatta ttatgtcttt acctgtactt caagcaatta tttatatagt
     5821 tggtttaatt ttcttgatgt atatggcttg gaccatttgg catgataaac cctcaacaga
     5881 tggagaaact caaattatgt ctccaatgaa acaagtaagt tttgctttat cagtttcatt
     5941 actcaatcca catgctattt tagatacaat tggagtaatt ggtagtagtg ctgcattata
     6001 tagtggcagc aataaaattg cgtttacaat cgcttgtatt agcgtttcat ggttatggtt
     6061 ctttttactc gcaattttag gaaaaatggt tggatcaatt gataaaaccg gaaaattatt
     6121 aacaataatc aataaaatat caagcataat aattattatt gttgctctga tgatattaca
     6181 aaaacttatt caattattat tctaaagcgt tatgtagttg tattttaaaa gcgttataca
     6241 aaagacatac ttgtgtctat gtataacgct ttatttatta taaatttgga tcaattaact
     6301 caacaaattt aaatgttcca ttgtcatatt caaatttcag gatattacaa ttaccgatat
     6361 tatgctttaa tgcttcatct agtttaaggc aatatcttaa aaatactccc attattgttc
     6421 catgactcac gactaatgca ttattctttg tttgagacat aatgtcatgt aaagttttca
     6481 caatgcgttc ttcaacttgt tgccttgcct ctcctttgaa aggaacaatt cgatctccga
     6541 ataagtcctc aggcttgtat agattatcaa atagatagac tgactcacct tcaaatattc
     6601 caaaatgcca ttccttcagt cccttaaaac gttgatacga ttggttaggt gcaacatttt
     6661 caagtgtgtc acttgcgcgt tcttgcgttg atgatgcata taaatcgaag tttatcccct
     6721 tagtttcata ataactacgt gccttttgag cttgagcaat tccaagttct gttagcggcg
     6781 aatctccaaa tccctgaatt agtcccttaa aattaaacaa agtttgtccg tggcgcatta
     6841 tatataacgt tttagccaac aataacacct cttcaataaa aagtattgat agctcaaaat
     6901 gtattctctt tttattaaaa tgagtaccaa atatattaaa ccattattta aactttttcg
     6961 gtagcgaaaa cctttgcttt tgatgtacat ttttttcata agtaatgatg attttagttt
     7021 aaaagataat gtgacggggt aaaacgcaat gaagagtaca gaattttaaa ggagtgagtg
     7081 tttatggata atcataatca atgtaattat gtgaatccac aaaatgtttc acttgactgg
     7141 gaatgtttta taattagtaa aagtgaaatg ttgttagatg gtgtaccaaa tgaacttatc
     7201 aacacttggt tagataaaga cattattaca ccattttcga taagaaatga tgaaataaac
     7261 tttaaaacaa aagatatttg ggatgcacta atacatcata attggtacta ctcaaattaa
     7321 tttgccatat ataagctaat agctctattt gtagtttcaa ttaattgatg atctgtgtaa
     7381 ttagacatca catatgctct aattacttgg tgtctttctt ctaatcgagg gaatgcttta
     7441 tcttcactaa cgtgttcggc caatatacca aatggtgtgt tatcattttg aaaacccatg
     7501 atgaaattgt aaaacgtcat aagacttgcc cccttcattt attaagcgct atttaaagtg
     7561 ttattattaa tcttatcaga aaactagtat ttgtaaatgt gcaaaagtga ttttgcacat
     7621 ttttttgata tctgattcta aaaatattaa cgaattatac aactttaaga gtttttagta
     7681 tgtaaatatt cttttcatat taatacaata cacccctata tatgctcact tgtttgaatt
     7741 aataaacttt agcattctgc aaataatatt ttaaaaataa ctatactttt tatttaccta
     7801 ttgaaaaata gtgttatcat atttattaag aagctatttt tatactagta ggtgtcagtc
     7861 atgaataaca ttttgttaaa tgctatcaat atagttatta ctaccacttt tgttatcttt
     7921 aatattttaa tcacatataa taaagattta gatgatttat gttggctcct gcctggtatt
     7981 atcatttgtg gtgtgatact catcgtatcc tttaccattg caatgataac taaaaactgg
     8041 ttaagtgaaa tattattttt tataaatatc gtactcgttc tctattacat ttatcctatt
     8101 ttttatagtt ttataggtta ataattacgg tatataagga gggtttcaca tgtctttaca
     8161 ttttgcaatt ctgttttggc tagcattaat tttcttagtt gccgctacgt ttatactcgt
     8221 attaatgaaa aaaactagca aagaatctaa aaaagagtcc tatttaagtt tcactgtcat
     8281 tctctatatt tttggattcg ctatattaat acatttatat ttggtgtgct ataaatgaaa
     8341 ttcattttat atccagtctg aaacgtctca atgatgtttc agactgtttt ttattcttaa
     8401 tagacatttc acactctcat actttaatat ttaagatatg ttttagtctt acaagcttat
     8461 taaagattac aattctaatt taagactatt tacatatatt tcttcaacaa ggagctttta
     8521 agtgaactat attaaacgaa cgataatttt acttatatta ttcgttgtcg tatccccaat
     8581 aaactcacca aaaacaattg cagataataa gtattcagaa attcaagatg acaaattcca
     8641 attacaacct ggtgatatta ttgttacaaa aggccctgtc atgtggggat tttttggtca
     8701 ttgtagtatc gcgattgatg ataaaacgat tttacaaatt gaggggcctg gcgacaaacc
     8761 aactacacaa tccttcgaat cttttaaata taattatgca agtggcaaaa atgattggat
     8821 gaaagtttat cgttgcagct atcctggtgc aggtaaaaaa gcggctgaat gggttaagaa
     8881 aaactatgag aatacaaatc atcgttatct agttacatta aatttgaaca gcaaaaaatt
     8941 tacgtattgt actaaaatta tctaccaagc ttataaattt ggtgtaagtg aaaaatcagt
     9001 taaaagttat ggactacata ttatttcacc ctatgcaatt aaagataatt ttatagatcc
     9061 atacaaacta aggcttgtta aagcttatta acacagtttc atcaaatagt taaattagtt
     9121 tgtgttttgc aataaattgg gtatagatta caaatgatat ttcaggaggc ccaatatttt
     9181 gaatgaacta actaaagaac aaaagtatac aatagctaaa ttttacaaat tatatattga
     9241 acgttcgaat aaaggagaaa cagaaacagt cgctaacttt tttggcgatg ctaaagatgc
     9301 acgtgaaaac tatttttgcg atcgtgatta tcaagacttc ttaactaact gccaaatttt
     9361 aattcaaaac aaatacttaa ctggtgaagt tttggacgat aacatttaca atatttctat
     9421 tttaaacaag acatttattg aattcgaaca aaattttggt tgaacatgaa tgtctcaact
     9481 ataaactttc taaccctatt ttaaagcagg agtgaaaaac atacatgagt gaatggcata
     9541 ttaataatca atctacaaaa ccttttttaa tacaacaagc ggaaaagcaa ttgtctattg
     9601 ttaaaccgtt gcctaatggt agcttccaaa tactcacaga aataaatttg gagaatggca
     9661 atattagtaa catcgatcat tctttacttg tttcagtaga ccccaaagca ttagaaataa
     9721 gtatatttga cgctgtaaat taaacgcaat atttttattt aattatttta atgtttcgcc
     9781 ttttaaataa aaacaagatt actgaattat tatgagattt taaaaacaat aaatattctc
     9841 aataattaat cagtcttaat aaaaaattaa caaaaagttg attagttaaa ttattaatta
     9901 agtagtaaga tattatgcga tgtggtatcc tattattgtt aaacattttg agtgtttaac
     9961 attacttctt gttatcgcca tatacagact ttaattctcg tcattattaa agttctgtat
    10021 tggcgctttt ttatattcaa aatcaaaagt cggacagatg aaaagtaaaa aacttttcca
    10081 tcagttcgat ttattttaga accattatat attgttaaat ctccaactaa atttattgga
    10141 caaaaaaata acggctagtg cttttaccac accaactgtt atcgatttaa agcataaaaa
    10201 aacagcccta tagtaaggac tgtttgaata agttaaaatt acattttaac tacgtttgca
    10261 gcttgctcgc cacggtcgcc ttcaacgatg tcgaattcaa ctttttggcc ttcttctaat
    10321 gatttgtatc catcttcagc gattgctgag aagtgtacga atacgtcgct accatcttct
    10381 ctttcgatga aaccaaaacc tttttctgca ttaaaccatt taactgtacc gttattcata
    10441 ttgaatacct ccgtcgtgct tttgcactaa atattttgta acaaattcat aaatgaaaag
    10501 gagaatattc tatataaata actcacattt ttcttcacgc tctttattac ttaactttca
    10561 ttatacactt ttaaatatag aagtaaagca ttattttatt ttctgtgata atacacattg
    10621 aactatatag taacattcca ttttaatact aaatttaaaa tttttatttt ctaatattaa
    10681 atacacttac ttttgatact atttactttt ttaattctga attgataata aatcatatcg
    10741 tgatctaaaa attataaaag tgccactagc agcagtgaca cttttacaat gagcattatt
    10801 gacctgaatc agcgttgtct tcgctccaaa tatttaattt ctcttttttt gcttgtgctt
    10861 cactttttct taaaagttgt tcatgtgtat tgttaggttt ataaacataa gcaactttag
    10921 ccaagccttg acgaactaaa gcttcgttta ccatttttcc atcagcataa atatacgcta
    10981 agccacgtcc atatttatca gttctttgac ctttgtcaaa ctcgacttca attttctttg
    11041 cattttctac catttttttc gtaaatgcac ttgcttcagg accatatttc tctacacctt
    11101 ttttaggatg ctttgtttca ggtgtatcaa ccaataatag tctgaatgtc attggttgac
    11161 ctttgtacat taatttaacc gtatcaccat caatcgcttt aattaatgtc gcaggttctt
    11221 tatgtaattt tttagttgaa gttgcactat atactgttgg atcttcagaa ccacttctat
    11281 ttacgccatt atctgtttgt gatgcatttg ctgagctact tagacttgaa actacaacta
    11341 aagttaacac taaacaacta gtagcgaaat agaaaaacct ctttgcgtat tgccctttcg
    11401 aaacattact gatagccatc cctataagta atattgaaac gattgccata catatgccag
    11461 cacttaataa gtattctgtc ataactaaca cctctttctt tttagttaat tttaatatta
    11521 aacgttaatt aattataatt ctacattttt acaattaatt aataaaaatt taataattat
    11581 aataaaataa ctatacttac ttttatgtat tttctatatt ttaggtgcca gaacttatat
    11641 atttagcctt atttgtaaaa ttaaaaagcc tacaacatga atcgttgcag actatcatat
    11701 cgagacaaag aggtttatta gtgtctctat atctatgtgg aatttgaaaa gattatttat
    11761 aatatttacg gggataaatt gtcatttaaa aacgagatgt ataacttata atttactttg
    11821 gattagcgtt gtaatccatt ataaacctta tcaatttgtt tattgaaata gttttgatgc
    11881 ggtttgctta atgtgttaac tttttgtttg gctttcatcc tagcatcagc actttgtgta
    11941 tctttatagt tctctactaa gctttgtgct tctttaacat gatcacgtct aataacgcgt
    12001 ttgtcacggt ctgtgaaatg tgatgagtca atttcactat aaccaaccat agctcctgat
    12061 ttaccgccca ttgttaatat gttgaactcc atcaagtttg aattgctatt agattgaaaa
    12121 atattaattt gagcatgttc aactgttata actttctctt ccgatacaat ttcagtaaat
    12181 tgcttagtat catttgcgcc actctctgtt actaatgcgt ttgcgtgatt agaatgccat
    12241 aattgagttg cacaaattac acccattgtt agtactaata ctttcctttt catttgttta
    12301 tgctccctta tttgctttga tataaaaacc gactttttca ccctgttttc ttcgccattg
    12361 aaaattatat accaatattt tagaaacaat aattaacttg cgatgaacaa actattaaca
    12421 ataatcttga gtattatatt tatcttaatt aataatatat ttatttaaga ttatgttact
    12481 tccaactttc aaagtagaaa aacggtataa tttgttgatg ggtgtttatt gataaactgc
    12541 aaaaaataca gcctacaacc acatagattg tagactatat ttaaaataat aggtatttat
    12601 catatctcgt aaacttagtg ccaattttta tactcgtggt gctggtaagc tacccttaaa
    12661 ttcaggaacg tagtgtgtag ggctataact tggaacagca tattgataat ttacattttt
    12721 gatatttaat gatggtttcc caattttata accatttgat tgtgaaaatg agaaatattt
    12781 cttcacacct ttaactactt tataagaata gaagtattta tagtcatatg ctttatttac
    12841 tttagcattt tgatgtgttg ctgttgtgtt attttggaaa cttggtacat gcatacgatg
    12901 tgaattgtga ccatatggtg gaattacctt gaaactattt atttgtggca caacacaaaa
    12961 gtgattaatt ttaatgctag catgccctgg tgtaacaaat ttatgcgcgt gatatccagg
    13021 aactgcaaaa tgatgcttga taattaaaga ttgagatgga tgtgtatatc taggcgattc
    13081 tgatggttta acaataaagt gtttattaat agaatccttt gcatgattta catgtttatg
    13141 tacatgtgtt gatttgtatg aagtaataac tttcttgtag tgggtttgcg tagtaatgaa
    13201 gtggtggttt actttgtttt gcgtaataaa tctatttact tctgggcctt tagcaacaaa
    13261 atgcttatca acttgttgtg aagctggatt gtttgttgct acattcacgc gattattgat
    13321 ttctttgtac tctggcacaa tgtttcctaa ctttgattct ggtacgacaa agtttttatc
    13381 tacaatttta ctagctgccg atgaatctgc aatcttattc aatttatctt cagaagctac
    13441 aaagttttta ttgtcagtta aattactaac atttactttt ggcgaattct taaattcatc
    13501 gtaagctgga ataattttat taattcctga ttctggaact acaaattttt tgtcaacact
    13561 ctctgtcact gatgctttag cgtgatttga attcataatt tgtgtagcaa atagcgtgct
    13621 catagttaaa actaataatt tctttttcat ctgttatttc tcctttatat agactcaata
    13681 ttataaccaa tataatttcc ctgttatatt cactaacagc attatatacc agaattttca
    13741 gtataataat taacttgaag taaacgttgt cttaacattt ttattgtttt tcagcttaaa
    13801 attaattatt gatattgata gttaagcata ataatttttt cgtaatataa agtgaaaaaa
    13861 gtaatagtcc acacctgttt agaatgtgga ctatactaga ttgcatcatt gaaatgatga
    13921 ctttgatatt atttattgct agtttaaaat gttgttattt ttacgacaaa ctcattattt
    13981 gctttgcagc catgcattaa ttatttgcca ttatatactt tattgatttg tttattaaaa
    14041 cgtttttgat gaccttcact taaagtattt actttttgtt gtgcatttat tcggtcttta
    14101 taactatgtg tatctttata attatcaact aacttttgag cttctctaac gtgttcacgc
    14161 ttaattacac gcttctcacg ttcagttaga tgagaagaat caatttgact tataccaacc
    14221 agtgatcctg attttctacc aggtaaagca gacgtttctt caaattcaac aacattatta
    14281 tgcgtgtgat ttgaagttgt tggtgattga tgcgttgttg tgaaatcatg actttcacca
    14341 tttaatccag ccaattgttt tggtgcatta taaataattt gttgctgagt ttgacgctca
    14401 actgtaggca ttggtacctg tgtcgttgtt tcagtatatg tagtcggtga tgttggtttc
    14461 tttgttggtg cagaaaccac aagtttcttg tcgttttcgt tgtcaatacg ttgtttatgc
    14521 tcaaaatcat attctaatga cactacaggt gttttcttaa ttttttgatt atccaaaaat
    14581 ctcgcttttc tttcttctgc tcttttatca tattcagctt tttgttgttc agaaacttct
    14641 tgagatgcag gtttgtgatt ttgagtattt aaacttcttt tacttctctt gcttcttttt
    14701 gattcatcac tttttgctgc ttcagtgtca gattttaatt gagccatatt tttatgattt
    14761 ttctcttctt gtttttcatc ttctaaaaca ggaatattat taggtcttgt tttatctata
    14821 tcactaaaaa attcatcaat tatggtttct aagtcttctt ttttattttc taacatcctt
    14881 ttgttaactg cttttttatt tgctctttct tcatctttat atcccataa
//
LOCUS       DANRDI010000058        14577 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.058, whole genome shotgun sequence.
ACCESSION   DANRDI010000058 DANRDI010000000
VERSION     DANRDI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14577
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.058"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..542)
                     /locus_tag="PZ377_001360"
     CDS             complement(<1..542)
                     /locus_tag="PZ377_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDM6358236.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKT"
     gene            complement(763..3564)
                     /gene="clfA"
                     /locus_tag="PZ377_001361"
     CDS             complement(763..3564)
                     /gene="clfA"
                     /locus_tag="PZ377_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDM6358237.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVKFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSE
                     SDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLL
                     ASIGSLLLFRRKKENKDKK"
     gene            complement(3827..4357)
                     /locus_tag="PZ377_001362"
     CDS             complement(3827..4357)
                     /locus_tag="PZ377_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDM6358238.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            complement(4496..5017)
                     /locus_tag="PZ377_001363"
                     /pseudo
     CDS             complement(4496..5017)
                     /locus_tag="PZ377_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5025..5180)
                     /locus_tag="PZ377_001364"
     CDS             complement(5025..5180)
                     /locus_tag="PZ377_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358239.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            5616..6344
                     /locus_tag="PZ377_001365"
     CDS             5616..6344
                     /locus_tag="PZ377_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDM6358240.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            6429..6682
                     /locus_tag="PZ377_001366"
                     /pseudo
     CDS             6429..6682
                     /locus_tag="PZ377_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6698..7021
                     /locus_tag="PZ377_001367"
     CDS             6698..7021
                     /locus_tag="PZ377_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358241.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            7300..8478
                     /locus_tag="PZ377_001368"
     CDS             7300..8478
                     /locus_tag="PZ377_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDM6358242.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
     gene            8738..9430
                     /locus_tag="PZ377_001369"
     CDS             8738..9430
                     /locus_tag="PZ377_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDM6358243.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            9427..10980
                     /locus_tag="PZ377_001370"
     CDS             9427..10980
                     /locus_tag="PZ377_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HDM6358244.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            10983..11939
                     /locus_tag="PZ377_001371"
     CDS             10983..11939
                     /locus_tag="PZ377_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358245.1"
                     /translation="MLKRTIKFILYLIVSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            11939..12706
                     /locus_tag="PZ377_001372"
     CDS             11939..12706
                     /locus_tag="PZ377_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358246.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            12673..13440
                     /locus_tag="PZ377_001373"
     CDS             12673..13440
                     /locus_tag="PZ377_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358247.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            13433..14068
                     /locus_tag="PZ377_001374"
     CDS             13433..14068
                     /locus_tag="PZ377_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDM6358248.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
BASE COUNT         4950 a         2421 c         2438 g         4768 t
ORIGIN      
        1 gttttaccta acattaatat ctgattttct aatttgttta attctaagtc gcactttaat
       61 tgctcctctt cattaaagtc tttcaattca gggttatctt tttttatatc ttcaatttct
      121 tttaagaact cctcactttg ttttttcaaa gtattaaata cactttcata tctttcatga
      181 gataatccta gataatctaa attatcagta tttccatttt ttattttttt ctcttcacta
      241 aaaaatttct tcaaagcctc atcttcagcc ataaatctct gttgatattt tttcatagct
      301 tctttatatt caggattatc aaatctttca ttatctaagt ttggaaagga ccatattaaa
      361 tcatccaagc ttttcttata ctcttctact gttctagatt tatttttatt attagtcagt
      421 ttcaacgact cagatacata tggattcttc tccccagaaa ccactgcact cgcacgatta
      481 ctttcccaaa tttgtgatac acataatgct cccaatgata aaactagcaa tttatttttc
      541 aactgtattt tctccttaat tttccctaat taacaaccta ccttacaccc tattttttcg
      601 ccaagccaaa ttatataaca tctacctttt taaaataatt aactgttgat gaattaatta
      661 ttaattatat tcaaacaata tatttggaaa atccaagtaa aaaagccacc ttttaaggtg
      721 gcttcttcat gaatcatatg attaatttaa tatcattatt acttatttct tatctttatt
      781 ttcttttttt cttctgaaaa gtagtaatga acctattgat gctaataatc cccaaattag
      841 tgacgtattt gcttcatctt cagaacctgt atctggtaat ggttctttac tatctttagc
      901 ctcattttta ttagaagcat tagtaccatt tttaggtgaa ttaggcggaa ctacattatt
      961 gttagaacct gactcggaat cgctatttga atcactttct gagtcggaat cacttgatga
     1021 atcggagtca ctacctgagt ctgaatcact cgctgaatcc gagtcactat ctgaatctga
     1081 gtcactgtct gagtcgctgt ctgaatctga atcgctgtct gattctgaat cgctgtctga
     1141 ttctgaatcg ctatctgagt ccgaatcgct atctgaatcc gagtcgctat ccgagtctga
     1201 gtcgctatct gaatccgagt cactgtcgga gtcggaatca ctatctgaat ccgaatcact
     1261 cgctgaatcc gaatcactgt ctgagtctga gtcactgtcg gaatctgagt cactgtcgga
     1321 atctgaatcg ctatctgaat ccgagtcact gtcggaatct gagtcactgt cggaatctga
     1381 atcgctatct gaatctgaat cgcttgctga atctgaatcg ctgtctgaat ctgagtcgct
     1441 atctgagtcg gaatcgctat ctgagtcgga atcactgtcg gaatctgaat cgctgtctga
     1501 gtcggaatca ctgtcggagt cagaatcgct gtctgagtcg gaatcactgt cggaatctga
     1561 atcgctatct gaatctgaat cgctatctga atctgagtca ctgtctgagt cagaatcgct
     1621 atctgaatct gagtcactgt cggaatctga gtcactgtct gagtcagaat cgctatctga
     1681 atccgagtca ttgtctgagt cggaatcgct cgctgagtcg gaatcgcttg ctgaatctga
     1741 atcactcgct gagtctgaat cacttgctga atctgaatca ctcgctgaat ctgaaccact
     1801 atctgatgta gaatcactac ccgaatctga accgctatct gaattagaat cgctgccaga
     1861 atctgaacct gggtcagaat ctgaatcctc tggaattggt tcaatttcac caggctcatc
     1921 aggttgttca ggaacaactg gtttatcgat accgtcacca gaacctgatc cgttattaaa
     1981 tgctacttcg ttgtcccatg acatagagcg ccaaattata ttcgagttat acccatataa
     2041 agttgaacgt aaagctaaat cacctttgct attcggatca atatgaccat taacaactac
     2101 tatatacggt gttgtaattt gatcatcagg cgtattaaac tttactttat attgatttgg
     2161 atttgggaat gtaatattca cactattagt gacatcctca aagttttctg gattcacaaa
     2221 gtaactttca gataaatcag ctgcattatc tactttatat actttaatac ttgtattttg
     2281 ctgatctatt aatgcattac tatccgtatt tggttttaaa ttacctgtta aaaccggcgc
     2341 aataacgtta tctccacttg gattgacata aattgtctga cgatacgtat tatttgtttt
     2401 atcgatttgg tcaattgtac ctttaataga taagttataa aacttaccat atttttcata
     2461 atctactaat actgttttgt ttgctgttgt actacctatg ccagtagcca atgtcacatt
     2521 acctgtcttt ttaacatttt cagggtcaat ataagcgggc atggtcaaag ttgcttttac
     2581 atcatcttta gtatttacat agtctgtaaa tgtataaata acattaccat cactatcgat
     2641 tacaccattt gccaatactt gatctccagc cataattggt ggcactttag cagttgaagt
     2701 tacaccattt aagtttaatt ctttaggtac agttattttg aatgtgtcac ctttaacagc
     2761 agaattaggc actgaaaaac cataattcag tttgacataa cctgcttggt gcggatacac
     2821 agtcgtacca gagtcaatac caactgtcac attcgtcaac tgattcgtaa tatctgtgcc
     2881 agctgccggt gcatctgcag ctactgccgc taaactaaat gctctcattc taggcgcact
     2941 tgtattaacc gcttgattaa ctacatcttt attacttgca tctgtactct gtggagctga
     3001 ttcattgttt gaaggtgttg cttcagttga agtatcttgc gttgttgaaa cattttccgc
     3061 atttgtagaa ttttgaggtg aatttacaga tgatactgta ttagtatcat tagaagtcgt
     3121 ttcattactt gtttgattca ctaattcctc cgcatttgta ttgcttgatt gagttgttgc
     3181 cggtgtatta gcttgattcg ttgtcgtagt agtagcttca ccagttaccg gcgtttcttc
     3241 cgtagttgca tttgttgatg atgattgtgt cgtttcctgt tgtgctggat tttgcgccac
     3301 actcgtttcg ccattattag tgtttgacga tgttttagta tcactcacgt ttgtgtcgtc
     3361 tgttttaggt gcagcactaa cgctacttga atcattactt ttgctttcgt tacttgcgct
     3421 atcagattgc gtaacactat tttcacttgc atctgcttct ttactgctga gtagtccaaa
     3481 accgattaac gtacctacaa gcactgaagc cacgccaatc gattttttcc gaattgcgtg
     3541 tttttctttt ttcttcatat tcattttatt ccctcttttt aaaaagtcat tttatattaa
     3601 ctatataccc tttaaagata tatttaatct ctgttaatgg aattatacac taaaattgca
     3661 ttatagcaat taatttgtat cgatatttta ttatccacaa taatacttta ctaacaaaca
     3721 ttttatttat tgctatttta agaattacaa acgacaacgt acgatttgat tgcaaacatt
     3781 ttttattatt aatatgaact ctacctaatg taatcctagc ttgaaatcat attttttcaa
     3841 aagcagatgt gtaatttatg gtacctgttt ttcccgctaa actgttcact tttaattctt
     3901 taattaaaaa cgcttcgtct ggtataccat catatggtgg ataaatgtcg tacatacttg
     3961 cgcgttgata tcctagattg gcataatacg tcggccatcc taatacactg ataaaattat
     4021 atccttttaa tattgcttct cgttctaatg cttgaatcaa tcgcttccca atacctttat
     4081 tttgatgatg aatatcaaca gatacaggtg ctaacactaa tccaatttcc cgttgtgcct
     4141 cgttatcaag ataaacttca cttagtaaac cgtgccctac aacttcattt tgaagaacag
     4201 ctactaattc taaggtattg tcatacgtat cacttagacg aatttggtct actagctctg
     4261 attcattacc ataaccatgt tcactatttt caaatgccgt tctaattaac tggtccactt
     4321 gagcaaagtc atgttgatgt atttgtctaa tttgcatctt tgcccctcct tttagttcta
     4381 tcttatagaa aaatgcatta caacataaag taatatttta tagtaatgac gccattattt
     4441 cattaaaatt gaatgatatt tttcgcaata aacaactacc ctcttaaatc aatttttaga
     4501 taaactgcaa atcataaatt tctttattat tataagtatt aaaacggtcg atttgacctt
     4561 ttaatataac ttcctattgt ttgcgactta cattatgata ataagtcata cgtacattca
     4621 aatcagcttc atgaccattg ttctcaatcg ttctttctaa ttcttcgtac acatttttat
     4681 taattggtaa catgtaccct tctttactag gtaaaatatg cacgtatttt ttctgaatga
     4741 ctttatcctt ccccaataaa acctctattg caattttttt agcagcaccg ccacctaaat
     4801 tatatagttt aatataatct ttatgatgat gctcttctgt tgccgaattc attatattaa
     4861 gttgatcctc ctccctttct aataaaattt ggttaaaacc taaagctggc agaaagctaa
     4921 ttttcatttg ataaagttga actgaaactg atacaaaata aaataaagcc ataataaatg
     4981 ttccaataga tcctatagct gaaataatgt taatcattaa gatatcaacc ctcccataag
     5041 aaatttatat gattaatttc aaactgaaaa agcattttac ttaatcgttt atatccaaaa
     5101 acaatcattt taaacgttat attcgactgc tcatataaaa agttttgctt tatagcctta
     5161 agtaattttt taaccatcaa gtacgattat tattacggca atctatttgt attgactaaa
     5221 aaatacaata acgcttttaa gagctcccat ctttgaccag tattttccaa attaataaca
     5281 cgtcttcttc aatacatatt tatctcatac aaataagttg atttttttat ttttacaaac
     5341 gtaatttctt aacaaatcaa aattttacat ttaaatcttt cgatatatta caagtttttt
     5401 taaaaaagta atatatttct atcaataaaa gtaatataat attagtaatt atagtttcat
     5461 taatttattg caacaataca taggatcatt attacaagaa ttatttaata tcatcttaga
     5521 atctcacaaa atatcaactt tgtttaatta ctcaaaatga taagtcattt agagttttta
     5581 aaataaattt ttgcgaaata aaggagacat gttaaatgaa aaaagtaatg gggatattat
     5641 tagcaagtac acttatctta ggtgcttgtg gacatcatca agatagtgca aaaaaagaga
     5701 gcactagtca caaaaagaaa gaaaatgaca atgaagaatt aaatgaagaa cttaaagaat
     5761 ttaaaagcaa aaaaaatatg gatataaaaa ttaaaggcga tactattgtt agtgacaaat
     5821 ttgaagctaa aataaaagaa ccgtttatca tcaatgaaaa agatgagaaa aagaaatata
     5881 tcgcttttaa aatggaaatt actgctaaaa aagacgataa agatttaaat ccatcttcta
     5941 tttctcatga ctatattaat atcactcaag atgataaaaa tacagtaaat aaattaagag
     6001 atggttatct tttaagtgat aaaaaatata aagattggac agaacataac caagatcaaa
     6061 ttaaaaaagg caaaactgca caagccatgt ttatctatga gttaagaggt gatggaaata
     6121 ttaatttaaa tgtccataaa tactcagaag ataaaacagt tgattctaaa tcattcaaat
     6181 ttagtaaact taaaaccgaa gatttttctc atagagcgga aacaagagaa gaagtagaaa
     6241 agaaagaaaa agaatttgaa gaagagtaca aaaaagaaca agaacgagag aaagaaaaag
     6301 aaaagcaaaa agatgacgac cacagtggtt tagatgaagt ataaatttgg tttgttgatt
     6361 gtcttacatt cagttattta atttttagag aaactgactt atttatagct tagccaacat
     6421 gatactataa agctatgatt aaaaataaaa aatctgtgtt taatggtgtt aaagaaatca
     6481 gtccccgtgc cggaaaatca taaaaaaaag atgggataaa tttgtaaatc ccgcattgaa
     6541 taatgcatta aaacaagaag aggctacatg gggaaatgta caaggtcaag tttcacaagc
     6601 attgatggga actggtatta aagattctac tgcaagaagc atagggtttt gggtttccca
     6661 agttggtcaa gcattaatat aaaaagagtg tgataaaatg aataatgaag aattagaaat
     6721 gagattgtta ttaatgaaac aatcaataga acaattacaa gaagaactag cgcctaactt
     6781 aaaaactaga gatttagtgt tattaagata tatgtattca tataaagaaa ttaatatgct
     6841 agattcttac ttgttccaat tggctacaaa taaagagcaa ataacaaaaa aacaatttaa
     6901 aacaaaattg gaaaacatta gagaagtacc agaaatacct attagacaag ttaatgatat
     6961 attagaaggt tataaaaata gtgagttata tgttgagttg ataaatagca tcctaaaata
     7021 aaaataaaca tactaagatt agctatgaag gaatctatga cgatagattt tttcatagct
     7081 attttttata gttatagaga ggagtagact gtccagactc ttggatttta aatccgtaaa
     7141 aaaaacaagt cagctttact ctcacctttt gaaattcgtt tgtagtatgt tgggttcttg
     7201 aaaccgtgta taggaaaatg aaatgagaaa ggttaagtaa agtttttagc ttctcaacta
     7261 ttcaaaggag gtttttttat cgattactta ggtgttgata ttagtaaaag gagtagtgta
     7321 gttgcacatt ataaaaatgg aaaattccaa aaagagtttt tcatccaaaa taataaaaat
     7381 ggctacaatt atttactcaa gtatttgaat gacttagacc acccacaact catttttgaa
     7441 tctacaggta tctattcaag aggtatggaa cgattttgtt gtgtaaatca aattaactat
     7501 attcaaatga atccgttaga agccaaattt aaaacgagcg ctctaagatc atggaaaact
     7561 gatcaggcag atgctcataa gcttgcttgt ttaggaccga cgctcaaaca aacaggcagc
     7621 ttacctatac atgagttaat attctttgaa ttaagagaac gtgcccgttt tcatctagaa
     7681 atcgagaatg aacaaaatcg acttaaattt cagattcttg aattactcca tcaaacattc
     7741 cctggtttag aaagattatt tagtagtcga tattcaatca ttgcactcaa catcgcagaa
     7801 atttttactc atccagacgt ggttcttgat atcgacaagg atgtacttat tacacatata
     7861 ttcaattcta cagataaggg aatgtcaatg gataaagcta caaaatatgc acttcaatta
     7921 agagtgattg ctcaagaaag ctatcctaat gtcgatagac attcctttct agtcgaaaaa
     7981 ttacgcttac ttattcaaca attaaaacaa tctattcatc atctcaaaca attagatgat
     8041 gccatgattc aattagcaca acaactcgat tattttgaaa atattcattc gatacctggt
     8101 attggtaagc taagcacagc tatgattatt ggggagattg gtgatattaa gcgatttaaa
     8161 tcaaataaac aactcaacgc ttttgtaggc attgatatca aacgatatca atcaggtcat
     8221 acacactgta gagataccat caacaagcgt ggtaataaaa aagcgagaaa acttttattt
     8281 tgggtgatta tgaatataat aagagggcag catcattatg acaatcatgt cgtcgattat
     8341 tactacaaac taagaaagca gcctaatgag aaacctcata agactgccat cattgcttgt
     8401 ataaatcgat tattaaaaac gattcattat ctggtaatga atcataaatt gtacgattat
     8461 caaatgtcac cacattagcc aaacgtacaa ttaaatatat tttaatacct tattcaaaaa
     8521 attaaaatga acggtttagt taagtaatgc ttattttaat tataagtact tgactaatcg
     8581 taagaaagag cctaggacat aaatcaatgt ctcgcgacca cagcttaatt tttggtgttc
     8641 attcatgact ttaaaaaatc cttattgcat aaatgtacat agtgtagtac tattcaaaac
     8701 gtaattatta cgatttgaat taagcgagga gaatgaaatg actaagactt atgacgtttg
     8761 gtggcaaaaa ggtcaagaat cagatgatga tatggcacga gaccatcaag aagcttggga
     8821 gagaacaata aaaatgcttg atacatctga catcgaaggg aaaacgattt tagatgtggg
     8881 atgtaatcaa ggcggatttt tacgacagtt atacgataca acaccgttta aaaaaggtgt
     8941 tggcatagat ttagcacgtt tatctttgga aaaggcagag acattaaaag gacaacgtcc
     9001 acttacatac tatttaacag ataaaccgca agaaacgaag cacgtgtttg atacggcagt
     9061 aagtacgtct gtcttgtact taatagaaga tattccgcaa catgcaaaag atttaaaaga
     9121 ggtattgaaa ccaggcggtg tttattacgc ttcattcgcg gatttaacta ataacccaag
     9181 tcgtcagttt atggatgaca cgattaatca atatggtgca acaccttctc agaatcactc
     9241 tctaaaacat atcgttgata gctttgtgga tgcaggattt gaagttgcag taatgaaaga
     9301 gcatgtacct gacgtgattg atttaacaca ttatagcgat ttttatttat caccgaatga
     9361 ttatttacaa acactatatg aagaatcgtt tttaataaaa gcaagtgtga aagaaggtac
     9421 tgagaaatga ggaaatgtgt attaatgacg gtagcagcaa gtgctacgct cttattggca
     9481 ggttgtggca atggtcaaaa agaagataaa gatgttacgg tatcgctacc tactgaagca
     9541 aaggcggata aacttgacgc gcaaggctat gatgcagcga tgcccgttta tagtgcagtg
     9601 tatgatgcat tagttaaata tgataaagat aagggtatta aagcaggttt agcagataaa
     9661 tggagcgttg atgaatcagg gaaagtttat gaattccatt tgaaaaagaa tgttaaattc
     9721 tcagatggtt cagcattaga tgctaaggac gtgaaattct cgattgatcg tgcgaaagcg
     9781 atgaacaaag attcgactgt agaaacgtta aaaaaattag ataaggtcgt tgttaaaaat
     9841 gagcacgtgg tccaaattag attgaaatct ccttcaaatc aagtgttaaa tgaattaaca
     9901 caagtgagac cgttgcgtat tatgagtcca cattcagtag aagatggtaa agtaaacggt
     9961 aaatttgaaa aagcgattgg aacaggtgca tttgttgttg ataaaactgg taaagaaaaa
    10021 acgacaatga agccaaataa atattttgac aacggtcacc cagtcaatta tcatcttgca
    10081 ttccaaacga ttgaagatgg ggactcaaga aattctgcag tacaaagtgg ttctgtagat
    10141 atttctggtg gtgctttagg tatgctctca gacgaacaaa tcaaacaaga taagaaaaat
    10201 aagaacttaa cgattgaaga tagacctagc acagtaagtc actttatggc atttaaccct
    10261 aaaaatgatg tattaaatca acgcacaatt cgtgaagcga taagtaagag catcgatgcg
    10321 aaagacattg cgggcaaatc tgtaaatggt ctgttccaga agaacgtaca atttgtgact
    10381 aaaaataatc aacagccaca cgattatgat atgaaagcgg ctgaaaggtt acttaaatca
    10441 gaaggatatc ataaaaacga tgacggcatc tttgaaaaga atggcaaacc tttatcattt
    10501 aacttagtca ttcaaactgc agagttccca aattggaaag ataaagctga aaaagtgcaa
    10561 cgtcagctta aacaagccgg tattaagtta aatgtgaaaa cgttagattc acaatcatac
    10621 tatgatacat tatggacgaa aaaagactat gatttgattt tctatagaac gtattcagat
    10681 gcattaatgc cttacaactt tataagttca gtgtttaaaa ataatgatgg tcaaccaggg
    10741 gtgttagctg atgatgaaac attaacgaaa cagctagacg atttcccatc aaccgtatca
    10801 aaagaagacc aacagtgttc atttgatgac atatttaaac actttaatca acaatactat
    10861 ggtgtgccaa ttgcttatcc aaatgagacg tttgtagtga gtgataaagt aaaacaattc
    10921 aaattctctg gacttacgga tgcaccaatt gattataaag cgttgaaagt taatgaatag
    10981 caatgctcaa acgtacaatt aaattcatac tttatttaat cgtaagttcg tttattatct
    11041 tcattttagt tgagaagaca tctggtaatc cagcgattct gtatctacaa cgtcatggtt
    11101 atacgtcgat tacgcaagac aatattgaag cggcacaaca tcaacttggc ttaggacaac
    11161 atgtgttact aagatatatc gattgggttg gacatgcact cacgggcaac ttaggatacg
    11221 gctttagtac gaacgaagca gttaccgcta tgataatgga agccatcgtg ccgacgcttg
    11281 tgctaatcat tgtctctagt tgtatcatgt tgccatttgg ctatattgtt ggttacttcg
    11341 ttgggacgcg tccgcataca cgttacgcta atggaattcg tggattcgcc caagtgatga
    11401 cctcaatgcc agaatactgg ttagctattt tattcattta ttatttaggc gtacgttggc
    11461 aattgttacc atttgtaggt agtgattcat ggcaacactt tgtgctgcca atcttcacaa
    11521 ttgttgttat agaagggtgt catatcttat tgatgacagc acatctgatt acacaaacgt
    11581 tagatcaaga tgcgtatcaa ctggcgcagt taagacattt ttcgttaaaa gcgcgtatca
    11641 tcgtacaaat taaagagata tttgcaccac taatgacgat ttcaattaac agtatcattc
    11701 atttaattgg aaaagccgta atactagaag tcatcttcag catgtctggt ataggtaaat
    11761 tgttgattaa tgctattaac caacgagatt atccactgat tcagggcatt gtcatcttta
    11821 tcattgtctt tattatgcta atgaattatt taggcgatgt gattattttg aagaatgaac
    11881 ctagacttcg acgacgtcat acccagcagt caggcaatga gaaaagaggt acgatgtgat
    11941 gaaaaaatat caaacgtaca tcgcaatagg ttcactattg agtttgatgg ttgtattaat
    12001 tacgtatggt ttaatgcaag acacgcaaca tttgaaccca cttgagtcac ctaatggaca
    12061 acattggttg ggtaccgatc aattaggcag agacttctta gtaagactga ttgtcggtag
    12121 tcttgtcaca ttgagtttaa caggcatagt gattctatta agcgtttgta tgggacttat
    12181 ctttggctta attgcaggca tagaaagacg atggttagat caaatcatca tgtttgttgc
    12241 cgatatgttg ctggctattc cgtcatttat tatcgcatta gtcatcttaa gtttagtaag
    12301 taactccatg ataggtttga tacttgcttt aacgattgga tggataggac gttatttacg
    12361 ttacttcaga aatttaacgc gagatattca aaaacgtcca tttgttcaat atgcacgatt
    12421 gagtgggaac tcaacattca aaacgacagt aacacatgtg attccacatt tattaagtag
    12481 tatattcgct ttggtaacgg ctgactttgg caaaatgatg ctcagcatat ctggacttgc
    12541 ttttctagga ctaggtatta aaccgccgac gcctgagtta ggaacaattc tttttgatgg
    12601 gaaaagttat ttcaacggcg caccgtggct cttcttcttc cctggtgtat tgttaggagg
    12661 tttcgcctta ttatgtcaaa ttatcaacaa aaaaataacg cagtaaatac ggtagtcaac
    12721 gtcaatcaat tatcgatttt agatcaagag aaagtattgt taaaggatgt tgatttgaca
    12781 gtaactaaag gtgcatttca ttgcattata ggtgaaagtg gcagtgggaa atcactgtta
    12841 acaagaacaa tacttggaat gaaacaatca caattatgtt atcaaggaga tattgacatc
    12901 gatttaactc aaacagatgc agtgtttcaa gatgttcaaa gtaatatgtt tcaaaatata
    12961 acattagcta agcatttcca atacatttat gaagccaatc gcacacatct cactaaacag
    13021 cgtattaagg aagatgtctt acagatgatg caattacttg gtttaagaca aggggaacaa
    13081 ttgcttgagc gttatccctt cgaacttagt ggaggtatgg cacaacgtgt cgcctttata
    13141 atgtcattaa ttagacgtcc gaactactta tttttagatg aaccaacgag tgcacttgat
    13201 caagaaaata ttaaaaagtt tatgcattac cttcttaggg cacaggagcg ctaccaaatg
    13261 accattgttt ttatcacaca tgatattaac ttagtgaaag attgtgccac acatattagt
    13321 attatgcagc aaggtaaatt gatagaaaat ggtgaggccg cgtcgatctt aactaagccg
    13381 acacataatt acacgaaaaa attaattacg attgcacatc ggagacaacc ttatgcttaa
    13441 aatagagaga ttaaccaaat atatagacac gcaactgata tttaaagaga tatcatgtac
    13501 aattaacgac cagcacttac tcataagtgg ggagagtggt tgtggtaaat ccacattagc
    13561 caagattatc gctggcttag atacggatta tcagggcgaa ttatatctta atgggcgctt
    13621 acgtgaatct tatacgtcta aagagtggat gaagcacatc caatatgtac ctcaatatca
    13681 acgtgatact ttaaatcagc gtaaaacggt attagctaca ttattagaac cacttaagaa
    13741 ttataaggta aataaacagc gttatacatc aagcattgaa gcagtgcttg atcagtgtaa
    13801 tttaccacac gatatactta atcataaagt ttcgacatta agtggtggcc aatttcaacg
    13861 cgtctggata gctaaagctt taatattaga accagagatt ctcatattgg atgaagctac
    13921 aaccaactta gatgtcatta atgaagaagc tatacttcaa atgttgattt ccttaaagat
    13981 gacacaatta atcattattt cacatgatac atacgtctta agccaatttg aaggaattca
    14041 gttacagcta aataaattga ataattaaga tcacaaatct taatatggtg aatatttaat
    14101 ggtacctaaa aaataaaatt taaactacaa tgtctaaatc catatgttgt ttcattagag
    14161 gatttaaaaa tgattataac actaaaagat ttcaaattat tatatttaat ataaatttac
    14221 atatgataaa cgaataacaa ttccaatata aattattttt tgattatttt tatttataac
    14281 tatatatttt atatgaaaaa tataagttat aataataagt ttaatattgc ctcgtggttc
    14341 tgagcttgaa cttatctcta aatcattttg agctacttat ctatcaattc atatattcta
    14401 taacaatatt tgtgacatca cgtgctattt catgaagtga ttttacgata tcaccttctt
    14461 tagaaaaaat atatttcagt aggcaccgac gtatacagaa tcatttgagt attaaaaata
    14521 aaaactagaa aaagaaaccg catcattaac tgatacgcag aatcatatta taaataa
//
LOCUS       DANRDI010000059        14564 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.059, whole genome shotgun sequence.
ACCESSION   DANRDI010000059 DANRDI010000000
VERSION     DANRDI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.059"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(61..159)
                     /locus_tag="PZ377_001375"
     CDS             complement(61..159)
                     /locus_tag="PZ377_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358249.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(178..1077)
                     /gene="sdaAA"
                     /locus_tag="PZ377_001376"
     CDS             complement(178..1077)
                     /gene="sdaAA"
                     /locus_tag="PZ377_001376"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDM6358250.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(1091..1771)
                     /gene="sdaAB"
                     /locus_tag="PZ377_001377"
     CDS             complement(1091..1771)
                     /gene="sdaAB"
                     /locus_tag="PZ377_001377"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDM6358251.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(1774..2817)
                     /locus_tag="PZ377_001378"
     CDS             complement(1774..2817)
                     /locus_tag="PZ377_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDM6358252.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            3368..3571
                     /locus_tag="PZ377_001379"
     CDS             3368..3571
                     /locus_tag="PZ377_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358253.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            3754..4689
                     /locus_tag="PZ377_001380"
     CDS             3754..4689
                     /locus_tag="PZ377_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDM6358254.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(4771..5739)
                     /locus_tag="PZ377_001381"
     CDS             complement(4771..5739)
                     /locus_tag="PZ377_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6358255.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(5872..6216)
                     /locus_tag="PZ377_001382"
     CDS             complement(5872..6216)
                     /locus_tag="PZ377_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDM6358256.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(6258..6668)
                     /locus_tag="PZ377_001383"
     CDS             complement(6258..6668)
                     /locus_tag="PZ377_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDM6358257.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(7048..9114)
                     /locus_tag="PZ377_001384"
     CDS             complement(7048..9114)
                     /locus_tag="PZ377_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDM6358258.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(9415..11154)
                     /locus_tag="PZ377_001385"
     CDS             complement(9415..11154)
                     /locus_tag="PZ377_001385"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDM6358259.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(11201..11890)
                     /locus_tag="PZ377_001386"
     CDS             complement(11201..11890)
                     /locus_tag="PZ377_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDM6358260.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(11883..12278)
                     /gene="cidA"
                     /locus_tag="PZ377_001387"
     CDS             complement(11883..12278)
                     /gene="cidA"
                     /locus_tag="PZ377_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDM6358261.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(12491..13369)
                     /locus_tag="PZ377_001388"
     CDS             complement(12491..13369)
                     /locus_tag="PZ377_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDM6358262.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            13536..13754
                     /locus_tag="PZ377_001389"
     CDS             13536..13754
                     /locus_tag="PZ377_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDM6358263.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            14143..>14564
                     /locus_tag="PZ377_001390"
     CDS             14143..>14564
                     /locus_tag="PZ377_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDM6358264.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFN"
BASE COUNT         4514 a         2801 c         2184 g         5065 t
ORIGIN      
        1 tcatcactcc ttttaagtta ttttagatat acgggggcgc ttttgcaatc actattttga
       61 ttagtatgca ttttccataa atctttcaac ttcttcagag ataattaaga agcatctatc
      121 tggtactaat gatccagaca gatgcttctt ttttatcaat attttattgt tatctcatta
      181 attattttta accatatctt cagctgtgcc aaagatttta cgtttaattg cttcgccagt
      241 tggtgtgcct gctagtccac ccaatccagt ttcacgtaat gatgcaggaa ggttacgacc
      301 aaccttatcc attgcttcaa taacttcatc aacagggatt ctactttcaa tacctgctaa
      361 tgctaaatct gctgaaatta aagcgttacc cgaaccaatt gcatttctca taacacaagg
      421 aatttcaaca agtccggcta ctggatcaca aactaaacct aataaattac ttatcgctaa
      481 tgccatagcg tgcccggatg cttctggtga tcctccgaat atagctactg ctgcagctgc
      541 ggccattgca gatgctgaac caacttcagc ttggcagcca cctgttgcac cagctacact
      601 tgcattgttt gctacgacac gcccaaacaa tgctgaagtg aataagaaat caatcatttg
      661 ctcttctgtt aaatcatgtg ttttttctaa tttaaaaagt gcaccgggaa tggtacccga
      721 ggaaccagct gttggcgttg cacaaataat acccatcgca gcattgactt catttgttgc
      781 aatggcacct ttgactgcgt caatcatttc atatccagac aaagcatgat gtgtttcatt
      841 ataatcacgt agtttagcag catcatgacc agtgtagccc gttacacttt caaccccatc
      901 acctgtcgtc cctttgatta ctgcgtctcg catgacatct aaattttgtt tcatttgcgc
      961 tcgcacttca tcacgtgatt taccgcttaa ttccatttct tctttaacca tgatatccgc
     1021 aaatgacata ttattttcta cggcataatc tatagtctct ctaattgaat caaacatgtt
     1081 tattccccct ctaatttata taggaaacgt ttacgtcact gtatttctct ttaattgtat
     1141 ttaatgttga ttctgagatt gctttattta atggtattac aaccaagcat ttatcttcat
     1201 ctatcttaat aaattcatct ttacagtcta atttcatatc gttgatatca ttgatgaaat
     1261 gatttacttg tgctttagtc atatttccgt caacaactaa aattggtaat ccatgattta
     1321 aatctacttc tagtccattt atatgaatac ctttaatttt aattgtacca ccaccgattg
     1381 aaataccgat aatttcaatg tagcgaccat cattacgaga tgatttgata taagcacagt
     1441 ttggatgttg accaatacta tcgccttctt cttcgatgat atctatttta ataccatcat
     1501 cagctgcaat ttctaatgaa gatttaattc ggttatcaaa tgttgaatat cccattgctc
     1561 cacccacaat agcgacatct gtaccatgtc cttggtgtgt ttgagcaaat gattcataat
     1621 aatgtatttc aatattttta ggaatatctc ccaatattgc gcgtgctgaa ttcccaatct
     1681 ttactgcacc agccgtatga gaacttgaag ggcccatcat aactggtccg ataatatcga
     1741 aagcactttg ataatcatag ctctttgcca taattaaaca ctctccttaa tatgattctt
     1801 tttgctcggc attttaaagt tgatattcat taaattaaac ttattaatta gtgttccaat
     1861 aatataggct aagacaatgc tgacaataat cactgttgcg attgttacaa ttgatgtcac
     1921 tgcattatta aagccaaaca atacgatggc gcctgcaatt ggtgttgcca tacctttgac
     1981 acctattact agtccgctaa atgtcacgat acatgcgttg acgaccccaa tcagtgcatt
     2041 tgtaccatat agttgtactg gatattgcgc tattaaatca atttgcgtca atggctcaat
     2101 acaaactgca aatgcttttg acggtccacc aatgtttaat tttcggaata aaataaggtt
     2161 aacaaatgag ctacctgtac atgttagtgc tccaatagcc ataggaacac ctgtcagtcc
     2221 taataaactt gttaatacca ttgaacttag cggtgtcata cctgtaacag gaatcactag
     2281 tcctaaaatg accgctaatg catatggatt gttatcacct accgcagtga cagcactacc
     2341 tatttgtttt aatgttgcta gcacaccagg tgtaatgatt gatgcaagtc cgaaagcaat
     2401 tgctggtgca aataagatca ccacaattaa gtccaagcct tctggaactt tcttttcaat
     2461 ccatttaatt aaaaaagcta cgccataagc tgcgatgaat gctggtaata atttaaagtc
     2521 atgtaatact aaaccaacaa tgaccgcaaa tactggtgca acgcctaagt ttaagcacgt
     2581 tagaatacct actgcgatac cgcttaaact tcctgctaaa tccccaatat cttgtagaaa
     2641 tttaatatca aatacgccac caatagcata acttaagaat gcttgtggta gaaatgtcgc
     2701 acaagctgca ccggataatg cttgtagtcc ttgtttaccg tacggtgcat actttaaaaa
     2761 tagcgtcatg atcactaaaa ccaagactaa tgtgcctaca cctaacagaa tatccatttc
     2821 ccaaaaacct ctctctatgt ttattttatt ttcagaccat aaacatcgta cacccttaag
     2881 aaaacgtttt caacttttat ctgttatcaa atcaaatatt taagtgaaat atttgatatt
     2941 tgtgaaagat ttttaaaatg gattgtttca aaaactactt atgttgtcgt taatatttac
     3001 taattaactt tttactctat atttcaaaca gttgtgtgac agttttttga taactttttt
     3061 acatctgaaa gtaagtaata tttctaaaaa cttttaatat ttatacactt tatctttcga
     3121 gctatttatt ttttgttttt gtgctgtgtt tataaaaccg cacactatat tcaaaaataa
     3181 cttattacac atatatttgc aataagataa ttaaagtagg atattatttt tagttttctg
     3241 ataggaatga tgatagtcta taggttgaat ctttactttt ttttaaagct aaatttacat
     3301 caacttaaca atggttggtt atactgaaga tgaaaatatt tagtataact tagtggaggc
     3361 gataaaggtg caattgagtc attccgttaa agttgcaatt tctatctatt tagcacttat
     3421 ctttataacg ttcacttctt atttagtcat tattttatat acgagtatga ctggacatga
     3481 tgtatcacat ttcgtgttag atagtcagca ttctcatcat ggatctctta cgcaaaaaca
     3541 tttgagtctt cctgaaatct catttaaata gtttatcctc tgtgtttcaa cattcatttc
     3601 ccatatcgat tcatttatct atcatctaga ccactacatc ttagatgatt tttttatttt
     3661 ctcatttcac tctttcttaa aagtcgatat aatgaattaa atcattatca tacaccgaca
     3721 tattttatgt tgttggtgtt aagttttaaa ggggtgagat acttggcgaa taatcattca
     3781 gctttgcgtt taaaaggaat tatacttgcc attgtcggtg cttgtttatg gggattaggt
     3841 ggtactgttt ctgatttctt gttcaaatat aagaatatta atgtcgattg gtacgtcact
     3901 gctcgacttg tagtcagtgg tgttttctta cttattatgt acaaaatgat gcaacccaaa
     3961 cgttcaatat ttagcgtatt ccaagatcga cgtatgttag gcaaattact tatcttcagt
     4021 atactgggca tgttagtagt acaatatgct tatatggcat ctattaatac aggtaatgct
     4081 gcgattgcaa cattactaca atacattgcg ccagtttata ttattatttg gtttgtcata
     4141 agaggcgttg caaaactaac attatttgat gtgcttgcta ttatcatgac actattagga
     4201 acatttttat tattaacaaa tggttcattt tctaatttag tcgtcaatcc tgcaagttta
     4261 ttctggggta ttttagctgg tgtagcactc gctttttaca caatttatcc ttcagaccta
     4321 cttaaccgct tcggttcgat tctaattgtc gggtgggcaa tgcttatttc tggtgttgcg
     4381 atgaatttac gccatccaat ttggcacatt gatatcacta aatgggacat atcaattata
     4441 ttatttttaa tctttggtat tatcggtggt accgcactcg cattttattt ctttatcgac
     4501 agtttacaat acatatcagc gaaagaaaca acattattcg gaactgttga acctgtcgta
     4561 gccgttatcg caagcagtct atggttacat gtggcattca aaccatttca aatcgtaggc
     4621 atcattctta ttatgatttt aattttatta ctatcactta aaagacaacc tgaaacatta
     4681 gatgaataag aaaactctga taatcacttt agcaagtaac tattatttaa caacgtagtt
     4741 accttatagg tgatatcaga gttttttatt ttagttaata atatttttca cttggtataa
     4801 aaagcgtcgt cgctctggta tcggaaatac tggataaata tggaattggt aatatccagg
     4861 tataaagtca tgttcgatac ctttcgcact caacaattgc gataagttca aagcatctgg
     4921 gtacagtact tcttttgtac caacagttaa tgtaatacga cctaaaccat ctaagtcacc
     4981 attaattggt gatactttgt agttatctaa aggtgtgtcc cctgcccatt gttcagctaa
     5041 gaacacactg ccatccacac ctaccattgg gtctttcttt aagtagtcag gaatttcagg
     5101 atgctgcatc gttgcatcta aaactggtga aattaataca atatgtcctg gttgcacaat
     5161 atgtttttct tttaacaatt gagcaaatga taaagcaatt tgaccgcccg cagagtcacc
     5221 cattacaacg atttgtttag aatctgctac ttgattcaat aaatcatggt acaacttttc
     5281 aaaaagcaca tacgtcgctt gataatcttg atgcggaatc ttcggatata ctggcatgat
     5341 gactttagca ttgagtgttt ctgcaagttc atcaataaat tcgaaatgaa ttttgagtgg
     5401 gtcttggaac catgcgcctc catgtgcata taatacaacg cgttgatgct tatcttgttt
     5461 atcgttaacg gtataaacgg ttgaaccgaa gtgcttatcc acttttactg gcatcttaaa
     5521 ctgataattg gttccctcat attcgcctgc tgtctgtggc gccatttttt ctaatgcttt
     5581 catcgcttct tctttataca taaaacgacg cttagctcta gataatcgta taccttgttc
     5641 taacatataa cttttcacac tgcgtttttc tttaattcta atatgtgcgt atgctgcaac
     5701 taaaaatcca aacgcaagtg tagaccattt tttacgcatt tgtcgtcact cctctttgat
     5761 gatttctact atagttctac tgtaccatgc tgattctaat tactaccaat atttactggg
     5821 aaataagtta tgatgtctac ataatcttag caatgctaat catttgcgat attaatgctc
     5881 atcgacatcg ttatttacac tatcattcat ttgcttaaga tgatgttcaa aacgttgcat
     5941 atcgataaaa cgcccttgac gatgcatttc ttttccattc ataaaaatta aatccacagg
     6001 tacagtgaaa atatttaatt ctccagcaat agcttccacc tgactttgat taatcacagc
     6061 taatggcaca ttgggatatg attgcatcaa gtcttcaatt tgtggtaaaa cggcatgaca
     6121 cacgctacac tgatcgcgca tgacatgtac aactgctaac ggatattgat gaataaattc
     6181 acggaatgct tcgacactat ttaattgctt aaccatttcg aacaccttct tactttttat
     6241 attttaaaat gaagtcatta ttttgttgct aaaatgtctt gaaacacttg tggcacagag
     6301 gttatcttct tatcactcaa tctgtaaaaa ccgcctgtct gtgtaccaat acaatgcacg
     6361 ccatgtgcgg acacaatttc aaactgaaac acccatttca tcttgcttgc tttaactaag
     6421 taaatacgac ctactggttt gtcatgtatt gtgacaggta ctttatactc tgcttccgtt
     6481 ttcataagta taggtgaaat gccttgattg atcattgttg agtaattcat gtgctgatta
     6541 ataaacgctg ttctcaaatc ttcgaaccat ctaatataaa cgatattact aacgacaccc
     6601 atcgcatcaa tgtcgtaacc attcacttcg atttctttct ctgcaactaa tagaccttct
     6661 gttgtcataa tgacaaatct ccttatctta caaatagatt taaaatcatg ttaatttatt
     6721 tttattactt tcatgataaa aatacaagca attgaacttt attacggaaa atcaaacttt
     6781 tcaatgagac tattaatttg gctagtcgcg aagatgcatt tttctaaaat gtgacaaaaa
     6841 cataagcaat gttttctata tctaacacta atatttaact tattatcatc aacacacctg
     6901 ctatacatca ttgcgcacta aaaaggcagt agcttcattt taagctactg ccttttgtaa
     6961 cgtattctct ttcaactcgc tacttcgcat acgcactacg aatgactaaa agttcattca
     7021 tttgatatgc tgtttcattt ttaatcatta tttgactgtc ataattagct tacctggatc
     7081 agcatcttgt acatcttcaa tgacaagttc tttattttca agatttgtaa taatcattgg
     7141 tgtaacgatg cttggtgcgt gtgctttcaa gtatgctaaa ttcactttca ttaatggttg
     7201 accttgtgtt actttttcat caacgttaat cagactttcg aatccttcac cattcagttt
     7261 cactgtatcg ataccaatat gaataagtac ttcgacgcca ctttcagatt ctaatcctat
     7321 cgcatgtttc gtagggaaga ttgttttcac tgtaccatca aacggtgcta caatttcacc
     7381 tttttcaggg ataaagccaa caccatcacc catcatttta ccagcgaaca cttgatcagg
     7441 tacttctgat aatggaatga tttgaccgat acctggtgca tagatgtctg ttgttccaag
     7501 tgcttctacg tgaactggtt catctgacat ttcttcagtc actgtcgttt cacttggttt
     7561 cgtaatttca ccactcataa tcttggccat atcatgttta atttgatctg attttggacc
     7621 aaagatagct tgcatattgt ttccaacttc taatacacct gatgcgccta aagctttaat
     7681 acctgctaca tctacttttg atttatcaac cacttctacg cgtagacgtg taatacatgc
     7741 atctaaatgt ttaatgtttt cttttccacc cattgcatct aagacatcaa atggtaattt
     7801 tgcgacacta gagttacgaa tttcagtttc ttcatcttca cgacctggtg ttttcaattt
     7861 aaacttacga attgcaaagt cgaataagaa gtaatacacg atagcatata caataccgac
     7921 tggaataact aataatgcgt gtgaacgatc ccagtttaat aaaccatata aaatataatc
     7981 tatgaaacca cctgagaatg tcataccaat tttaacgcct aataaatgca ttactaagaa
     8041 tgatgtacca gctaataata cgtgaattcc ataaagtact ggtgctacaa ataagaatga
     8101 aaattctaat ggctcagtga taccagttaa aaatgcagtt aatcctgctg ataacattaa
     8161 accacccacg acttttttac gttctggtcg tgcattttta taaatagcaa atgccgccgc
     8221 tggtaaacca aacatcataa atggatattt accagtagta aatgcaccag cagtaaatgg
     8281 tacgccatct ttcaattgtg ccatccaaat acgttggtca ccacgaacca attcacctgc
     8341 gtgatttgta taacttccga attcaaacca gaacggtgaa tagaaaatat gatgtaaacc
     8401 aaatggaatt aatgagcgtt caataatacc gaatataaac gttgttaatg ttaaattttt
     8461 atttaataag aaattcgata aactatttaa tccatcttga attggtggcc acgcaaagct
     8521 taaaagcaca cctgttgcga ttgctacgac cgatgtcaca atcggtacaa atcgtttacc
     8581 tgcaaagaat cctaaaaatg gtggtagtgt aatattataa aatttgttgt aacaccatgc
     8641 ggctaaagca cccatgataa taccaccaaa cacacccgtt tgtaacgttg gaatacctaa
     8701 tactaaagca tgtgctggtt ctttcgctgc ttgacttaat tcttttgccc ctttggcata
     8761 tgagaaaatg tcatcaattg taatgtgcaa cactttcccc attgttgcat tcataattaa
     8821 gtaacctact agcgctgcta atgctgcaac accgtctcct cctgctaatc caagtgctgt
     8881 accaactgca aataataatg gcaagttatc aaatacaact tgtcctgctg cttccatgac
     8941 cgacgaaatc attacaatga tatcgttttt taaccatggt gcaatttcta ctaattgttc
     9001 gttgtgcatt gcgttaccaa acgctaataa aataccagct gctggtaaaa tcgcaacagg
     9061 taacattaat gctttaccga tacgttgcaa ttgtccaaac aatttcttaa acatataaac
     9121 atcccctatc tcaatttccg cttccatgta caaaaaaagc aggcatgaac aaacaagact
     9181 attctcatag tgcggttgtt tgctcatgcc tgcattacca gtaacacgca agtgatatct
     9241 aattcatagc tattatacat atttgtaaaa gcgttttcaa caactttttc acaattttat
     9301 catcaattca gtacgatcat ttgtgataaa taaacaaaac ggcaattccc attttgttga
     9361 aatagaaatt accgttttta tagttcaatt caattgtgat aacctttaaa tcagttataa
     9421 gaaacgtttt gctgccactg aaattggtgg aatttgatct aaatctaaat gtttatcttc
     9481 agtaattgat ctaaatgccc acttaccata accaagcgct tcttcattta caattttacc
     9541 tggtaatggc gcagcattag gatcaacata tacatctaca atcgttggta catcttgtgc
     9601 taatgcctct tcgactatag catctacttc gctagcactc ttaattgtat aacctttacc
     9661 acctgctgcc tcagcaaatt ttgcatgatc catatcagaa aaatcaactg catattctaa
     9721 ttcaccagct gcttgttgtt catatttaat aaatgctaac tgtttgttat taagtacaaa
     9781 tacagttaaa ggtaaatcat attgtactgc tgtagcgaag tcttgcatta ccatttggaa
     9841 tgcaccgtca ccagcaattg cgatggcttg tctatttgga tatgcaattt ttgatgcaat
     9901 tgcacctgga agaccgcaac ccattgtacc taaccaactt gaaatgatga acttgttatt
     9961 tacaccaagg tttaagtatc gagttgacca aactgttgct gtacctacat ctgctgaaat
    10021 cactgcatca tctttaataa atttattgat tgatgccatt aatcgttctg gacgtaatgg
    10081 tttactatta ttatttttat cttgttccat ccatttatcc caaaccgctt tacgttctaa
    10141 cgttttgttt aagaatggtc tttcagcaac atgtttaata ttttcagtta actgatgcaa
    10201 cgcaatttta ctatctccaa caattcctac attaatattg aaacgatgtc cgatattttt
    10261 aggatttgtg tcaatttgaa tggctttaat atttttctta ggtaagtaat ccacatatgg
    10321 atagtttgta ccaaccataa ttaataaatc cgcttcctgc attgtttgat aagatgtttt
    10381 ggtaccgatt ttacctaagt taccgatact atatggatga tcatccggca agattgtttt
    10441 agctggtaat gaatgaatga caggaatttt agccatttca ataaattcac gtagctcatc
    10501 tttcgcatgt ttcgcaccta caccaattaa catgacaggc tttttacttt tattaattag
    10561 tttaaccgct tttttgatgt ctttatattt tggtgatact actgttggtc ttgatgtatc
    10621 tactggttta ttcgttgtat ctttaatttt ttcagttaat aagtcgttag gacaaataac
    10681 aacagctaca cctttttgtt catatgccgt acgaattgct tcgttaacga tttcaaacac
    10741 attgtcacct ttttcaattt ggtgattata aacggctaca tcttcacata atttttgtaa
    10801 atttgtttct tggaatgctt tcgttccaag tgctgtacta ttcgtttgtc cagataatat
    10861 taattgcggt acattatcca ttttggcatc atacatacca tttaataaat gaattaaacc
    10921 agggccaccg atacttaatg ccacaccgat tttaccagtt aattttgtgt aaccagcagc
    10981 cgctaagctt gctacttctt catgacgtac atgataaaat ttaaattgat ctctcactgt
    11041 acgtaaacta tcgactactg cgtcgattga gtctcctgga ataccataca agtgatctat
    11101 atcccatgct tgtaatgctt taactaatgc ttcatttgct tttatttttg ccattattac
    11161 taatagcctc cctttctgtc tatcatttta aatatctaaa ttaatagaat aataaaatta
    11221 gaacaggtat taacactgaa ctaataactg cagttaaaat catcccaatt gaactaaatg
    11281 caccggattc aatatccatt tctagtgctt tagctgtgcc aaatgcatgt gacgcattcc
    11341 catacgttaa tcctttggcg atagaagatt caaatcttcc aaatcttaat aacatcgaac
    11401 ctaaaatact accgattaaa cccgttgtga taataaaaag tacggtcatc gtatctgtac
    11461 cacctagttc atgtgacact tcgataccta ctgcggctgt tatagatcgg ggcaataacg
    11521 ttacaatgac gtctttagaa tagccaaatg ctttaagtgt taagaacact aacatgaaat
    11581 tcagcatgac gccagttaat acacttgcaa aaatgataga gacattgtct ttaattttct
    11641 ctctattttt ataaagtggg tacgctaaac atacgaccgt tgcgtttaaa atatggttga
    11701 tccaactgcc acctttcata tacccgttat aactaattcc aaagataagt aagacaaaaa
    11761 taattcctaa agatgcaatt aatgctggat tcaaaaatgg gttcggatat ttttgttgta
    11821 accttttagc gaaataatat aaaacgacag tcaacaaaat cattaataag gcttgcacgt
    11881 aatcattcat aagcgtctac acctttacga tgtttatgtt taacagacat tttttcagca
    11941 atataacctg aagataatgc aacgatacat gttcctatga taatgactgc gaaaaagagt
    12001 atataattta gcgtaatttc ggaagcaaca tccataatac ctacaactga cggtatgaag
    12061 aaaaagacca tcgtctttaa taaaaagttt gcaccgtctt ctacccaagt tagcggtaca
    12121 atcttaaatt gtaatagtaa ataaaataaa aatagaccaa caatactgcc ggctaaggga
    12181 agatgaaaaa tcttttgaat ttctgtgcca atataagtaa tcacaatgat gattcctagt
    12241 tgtagtagta gtttgattat taattggact ttgtgcatgg cgccatccct ttctaaatat
    12301 gtctaaattg ttacaataac tattataaag atggcgtaca caatataaaa atactctttt
    12361 tttatagttt gtattactat tttctatgga aacgctctct aaatttcact tcattttcac
    12421 aaaaaaacac tcattatttc aatgacccac gtaatgatgc gccttaaata atgagtgctt
    12481 tattaatatg ttagcctaaa cggtctttca aaaattctat ccatttcgtt gtggcatgac
    12541 ttaattgttt atccttcttc caaacaacac ctaattccca atgtacatga gcatcttcaa
    12601 tgcgtagcag cttcacatct ccatttagtt gctctgaaat tgatgttggt aaaatactaa
    12661 tacctaattc attcgtaact aaatcttcta taacatgcca ttgtgaaatt tgcgctacag
    12721 tattcggaac aaagccaacg tttttagcat tttcaataat tttatcattc aagtaaaagt
    12781 ctttattaaa taaaatgaag tcttcacctg ctaaatcttc gagtttaaca gtttcatatt
    12841 ttgcgagtcg atgctctctg ctcacgataa gtcgcaaatc ttccttatct agggtagtat
    12901 aatcgaaaat atgatgatcg actggcaaag tggtcacgcc tatatctact tcatcattga
    12961 taatttgctg ttcaattgtt ttaccgccat tttctatcaa attatatgta acatttggat
    13021 attgttgatg gaatgcacca agaatattga taagaatctt catattcatg actgccgaca
    13081 tgcccatgtt tatatgtcct gtttccagtc cattcaagcg ttccatttca gatggtaaat
    13141 aatcatacag tgcaacaatt tctttacttt tctcataaaa aatttgacct gcatcggtaa
    13201 gaattaaatg tcttttactt ctatcaaata agggcgtgcc catttcattt tcaatatctt
    13261 taattgcttt actaattgta ggttgtgcaa tatataatga tttggaagca ttagtcatgc
    13321 ctccttgctt aacgacttca ataaaatatt tcatatgttt gatatccact aacatcatcc
    13381 taactatttc aacacgttta tttcattata cacaacttct taaacattca ataaatttag
    13441 atttataaaa ttgctttata aaaagtttta tgtaaaatac acattctttt atggtattat
    13501 attaatggct caagtatatt aatcgaggtg aggtattgag cttttacgaa tttatgcaaa
    13561 gttttcttgg tgatgacaca ccattaggtg aattggttga ttggattaat caagatagca
    13621 atttccctag agaagtgaag agccatagtg aaatattgtc atattttcga acaaatccat
    13681 gtcctgaaag catctcagta cctgtaatta aacgggcact atcagttttc aaccaattca
    13741 caaatgtaca atgatacatg aatacatgga gtcacatcat ctcatgttag cgctctgatt
    13801 gcagggcgct atttttaatg ttattgctac atttagtata ttgtgattgc tattttcttt
    13861 cagttataga accatgtacc ttttttttaa atcattttat attttcccac taatatccgc
    13921 tgttaatcaa tcctgacatc cttgtatcac tatgacaatt aattgttaaa tacatgaatt
    13981 tctacatttt atgaaaaaat ccatttttat tacaattcaa cactttatat gacaacttca
    14041 ttacagttac ttttattgtt gattgcttac attgttttct aaaaaaaatt tgttatcata
    14101 attaacgttg aataaagaaa aaaattaact tgggagataa aaatggaata taaaaagata
    14161 ctaattcgtt tattaattgc ttttgcagta cttttctcag cagatttcac ttatcaatcc
    14221 gtcgaacaaa cgcatcaatc gcatgccgca gttaattatt atagtaaaaa ccaatgtaca
    14281 tggtgggcat ttaaacgtcg cgcacaagtc ggtaaacctg tttctaatag atggggcaat
    14341 gctaaaaatt ggtattacaa tgcacgtaaa tcaaaatatg cgactggtcg tacaccaaga
    14401 aaatttgctg tcatgcaatc aactgcagga tattatggac atgtcgcagt tgttgaacaa
    14461 gtatataaaa acggtagtat taaagtttca gaatacaact tttatcgccc attaaaatac
    14521 aatacacgtg tactaagcaa aaaggcagca cgtaacttta acta
//
LOCUS       DANRDI010000060        14249 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.060, whole genome shotgun sequence.
ACCESSION   DANRDI010000060 DANRDI010000000
VERSION     DANRDI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14249
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.060"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..376
                     /locus_tag="PZ377_001391"
     CDS             <1..376
                     /locus_tag="PZ377_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDM6358265.1"
                     /translation="DFAIQHSPNSIEGTIATLPTVQQNLGKMETLLLCARQFLWSTAK
                     GYQQYKDDSQIRNPTSASKVMVMNQGLEVIDLAMRIVGAKSLEMNRPLQRYYRDMRAG
                     LHNPPMEDAAYTNIAKSITDTF"
     gene            590..1498
                     /locus_tag="PZ377_001392"
     CDS             590..1498
                     /locus_tag="PZ377_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDM6358266.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            1897..2838
                     /locus_tag="PZ377_001393"
     CDS             1897..2838
                     /locus_tag="PZ377_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDM6358267.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            2959..3513
                     /locus_tag="PZ377_001394"
     CDS             2959..3513
                     /locus_tag="PZ377_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDM6358268.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            3585..4355
                     /locus_tag="PZ377_001395"
     CDS             3585..4355
                     /locus_tag="PZ377_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6358269.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(4527..5324)
                     /gene="fdhD"
                     /locus_tag="PZ377_001396"
     CDS             complement(4527..5324)
                     /gene="fdhD"
                     /locus_tag="PZ377_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDM6358270.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            5614..6396
                     /gene="modA"
                     /locus_tag="PZ377_001397"
     CDS             5614..6396
                     /gene="modA"
                     /locus_tag="PZ377_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDM6358271.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            6410..7081
                     /gene="modB"
                     /locus_tag="PZ377_001398"
     CDS             6410..7081
                     /gene="modB"
                     /locus_tag="PZ377_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDM6358272.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            7082..7687
                     /locus_tag="PZ377_001399"
     CDS             7082..7687
                     /locus_tag="PZ377_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDM6358273.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            7857..8861
                     /locus_tag="PZ377_001400"
     CDS             7857..8861
                     /locus_tag="PZ377_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDM6358274.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            8892..9398
                     /locus_tag="PZ377_001401"
     CDS             8892..9398
                     /locus_tag="PZ377_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDM6358275.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(9400..9894)
                     /gene="moaC"
                     /locus_tag="PZ377_001402"
     CDS             complement(9400..9894)
                     /gene="moaC"
                     /locus_tag="PZ377_001402"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDM6358276.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            9957..11216
                     /locus_tag="PZ377_001403"
     CDS             9957..11216
                     /locus_tag="PZ377_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDM6358277.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            11213..11698
                     /gene="mobB"
                     /locus_tag="PZ377_001404"
     CDS             11213..11698
                     /gene="mobB"
                     /locus_tag="PZ377_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDM6358278.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            11712..12158
                     /locus_tag="PZ377_001405"
     CDS             11712..12158
                     /locus_tag="PZ377_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDM6358279.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            12164..12397
                     /gene="moaD"
                     /locus_tag="PZ377_001406"
     CDS             12164..12397
                     /gene="moaD"
                     /locus_tag="PZ377_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDM6358280.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            12404..13003
                     /gene="mobA"
                     /locus_tag="PZ377_001407"
     CDS             12404..13003
                     /gene="mobA"
                     /locus_tag="PZ377_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDM6358281.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            13023..14045
                     /gene="moaA"
                     /locus_tag="PZ377_001408"
     CDS             13023..14045
                     /gene="moaA"
                     /locus_tag="PZ377_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDM6358282.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
BASE COUNT         5051 a         1961 c         2797 g         4440 t
ORIGIN      
        1 agactttgca atccaacata gccctaatag tattgaagga acgattgcaa cattgccaac
       61 tgtgcaacaa aatttaggga aaatggaaac gctattgtta tgtgcgagac aatttttatg
      121 gagtacagca aaagggtatc aacaatataa agatgacagt cagataagaa atccaacaag
      181 tgcgagtaaa gtgatggtaa tgaatcaagg gcttgaagtg attgatttag cgatgagaat
      241 agttggtgct aaaagcttag aaatgaatcg accattacaa cgttactatc gtgacatgcg
      301 tgcaggatta cacaatccac ctatggaaga tgcagcatat acaaatattg ctaagagtat
      361 tacagataca ttttaaattt taaaaggtaa atcgatcgtt gattggcata aagagatgaa
      421 atttgacgtt cggatttacc ctttttttgt ggggatttat agggatgtta agatattttg
      481 ttaaattttt tgcgaaaata aaaaataaat aacactagct attgtaaatg tatatgcaat
      541 cttgtataat ttttgaatga ttgataatca ttttcaatag gaggaaatta tgaaaaaact
      601 attattacca ttaataatta tgttattagt gttagctgcg tgtgggaacc aaggtgaaaa
      661 aaataacaaa gctgaaacta aatcttataa aatggacgat ggcaaaacgg tagatattcc
      721 gaaagaccct aaacgcattg cagtagttgc gccaacatat gctggtggac ttaaaaaatt
      781 aggtgcaaac attgtagctg taaatcaaca agtcgatcaa agcaaagtat taaaagataa
      841 atttaaaggt gttacaaaaa ttggtgatgg cgatgtagaa aaagttgcta aagaaaagcc
      901 agatttaatt attgtatact ctactgacaa agatattaaa aaatatcaaa aagtagcacc
      961 aacagtagtt gttgactata ataagcataa atatttagaa caacaagaaa tgttagggaa
     1021 aattgttggt aaagaagata aagtaaaagc ttggaagaaa gattgggaag aaacaactgc
     1081 taaagacggt aaagaaatta aaaaagcaat tggacaagat gcaacagtgt cattgtttga
     1141 tgaatttgat aaaaaattat acacttacgg cgataactgg ggtcgtggtg gagaagtatt
     1201 atatcaagca tttggtttga aaatgcaacc agaacaacaa aagttaactg caaaagcagg
     1261 ttgggctgaa gtgaaacaag aagaaattga aaaatatgct ggtgattaca ttgtgagtac
     1321 aagtgaaggt aaacctacac caggatacga atcaacaaac atgtggaaga atttgaaagc
     1381 tactaaagaa ggacatattg ttaaagttga tgctggtaca tactggtaca acgatcctta
     1441 tacattagat ttcatgcgta aagatttaaa agaaaaatta attaaagctg caaaataatt
     1501 cagctatata agttagtgaa atgagagtct gaaacatatc aatcttttga tattgtatta
     1561 ggctcttatt tttatagcta gaaagttaga tatttgtatt tttttaaata ataagtgccg
     1621 ttgttatcgt tcaatttaat taatgataga ttagtattat tatagctaaa gtagtatacc
     1681 tgagaaaata gctcaatgta tctctttatt aataagttat atcataatta ttttagtgca
     1741 tactttatgg aagggatatc agggaatggc tttcaattaa agaagaggtt taaaaggatt
     1801 acaacagaat gttatgattt tgtagaaaga tatataacaa cgttttataa aaacataata
     1861 ttgttaatgg aaaatgaaat gtaaggggga tttcgagtga ctaagaaagt ttattttaac
     1921 cacgatggtg gtgtagatga tttagtatct ctatttttat tattacaaat ggaaaacgtt
     1981 caattgatag gggtcagtac aattggtgct gattgttatt tagagccatc tttgagcgca
     2041 tcagtaaaaa ttattaatcg tttttcaaat gaagatattc aagttgcgcc atcatatgaa
     2101 cgaggaaaaa atccatttcc taaagaatgg cgtatgcatg ccttttttat ggacgcattg
     2161 ccaattttaa atgagccagt caaacatgtt gcttcaaatg tgagcgacaa agaagccttt
     2221 gaagacatta ttcaaacttt aaagagacaa tcagaaaaag taacattatt atttacaggc
     2281 ccgcttacag atttagcaaa agcactacaa aaagattcat ctatcgttca gtatatagaa
     2341 aaattagttt ggatgggtgg caccttttta ccaaaaggaa atgttgaaga acctgagcat
     2401 gatggttctg cagaatggaa tgcatattgg gatccagaag cggttaaaat tgtttttgat
     2461 agcgatatag agattgatat ggttgcttta gaaagtacga atcaagtacc gctaacgtta
     2521 gatgttagac aaagatgggc gaatgaacgc caatatacgg gcattgattt tttaggagta
     2581 agttatgcag cggtaccacc attaacacac tttataacaa attctactta ctttttatgg
     2641 gatgttttaa cgactgctta tattggtaac aaggacttgg ttcattcaat tgagaaaaaa
     2701 gtcgatgtaa taagttatgg accaagtcaa ggtaagacat ttgagtgtaa agatgggcgc
     2761 aaaattaatg tcataaatca tgtagataac aacgcatttt ttgattatat aactgcactt
     2821 gctaaaaaag taaattaaca gctgtgtaga ataattaagg ttttaattta tatagaacaa
     2881 cttattgtaa acttttcatt tcttaaagtt tacaatggtg ctataataat ggtcatgaaa
     2941 tacgaaagga agtaaaaaat gacaacaaaa cagttagtat atacagcttt aatgacagcg
     3001 attatcgcta ttttaggatt ggtaccggta attccactac cattttcttc agtaccaatt
     3061 gtacttcaaa acattggtat tttcttagca ggtgtgattt taggacgtaa atatggcaca
     3121 ttaagtgtta tcgtcttttt attattagta gttgctggct tgccattgtt atcaggtggt
     3181 cgcggtggca tcggtgtatt cgcaggtcct tcagcagggt ttttactatt atatccagtt
     3241 gtagcattca tgattggggc gattcgagat agattcatca atgaaattaa tttctggatt
     3301 ttattcgttg gtattttagt ttttggtgtt atagcattag atgttattgg tacattgatt
     3361 atgggcatga ttattaacat accatttacg aaagctattt caatttcatt agcttatttg
     3421 cctggtgata tattaaaagc aattgtagca agtttgattg gtacagcttt acttaatcac
     3481 tcgcagtttc gtcaaattat gggaataaaa taatcatatt taagatagta aagtaattga
     3541 ataagttgct ttgaaattta taaaagtgaa aggagtaggt gtcaatggct agtataagta
     3601 tgtcagacat atattgtaac ggcactatat ttgaaaatga cgacgagcag ttgatttatt
     3661 taacgccttc ttttccacaa cgatacacaa gtaacacatg gatatataaa aagacgccta
     3721 cccaagagcg atggctgaaa gacttagaac gtcaacatca attacataca aatcaaggtt
     3781 caaatcatta tgcgtttagt ttcccggaaa atgaacaact tgataatcat tggatggcta
     3841 tgtttaaaga tatgaatttt gaactaggta ttatggaatt gtatgccata gaaagtgatg
     3901 cgcttgccaa tttgccgcgt aactctgacg ttgaaattgc catcgttgac gagtcgcata
     3961 tagatgccta tttaaaagtt gcatatcagt ttagtttgcc atttggaaaa gactatgcag
     4021 atgcacatga agaaatggta agggaacatt atcaaaaaga tgtgattaaa cgcttagtag
     4081 cttatttaaa taatgaacct attggcgttg tagatgtcat tgaaagtgaa aattacattg
     4141 aattagatgg atttggtgta ttagaacaat ttcggcacca aggaattgga tctacaattc
     4201 aatcgttgat aggtgaatac gccatatcaa aaaatcacaa accaatcata ttagttgcag
     4261 atggtgaaga tacagcaaaa gatatgtatg caaagcaagg ttatgtctat caatcgtttt
     4321 gttatcaaat attaaaagaa gatattggaa attaacgatt gatgataggt ttgtattaga
     4381 ataaaaaagc agtaagctgg tttcgagtag aaatcaactt actgcttttt aaattgtttt
     4441 gagctactta tacttataaa aatagtgcgt ttaaattgtt gattcatgta gaatatcgtt
     4501 cattatgaca cactataatg aatatgttat tgttcagaat caatgatacg ttctggatga
     4561 ctgtatatat taaagccacc atttcgaata aatccaactg ccgtaatatt taggtcatta
     4621 gctaaggtta cagcaagcgt tgtcggagct gatttagata aaatgacgcc aacaccaatt
     4681 tttgcggctt taattaaaat ttctgatgaa atacgtccac taaaaattaa tactttatct
     4741 cggacagtaa tatgtcgctg aatacaaaat ccatataatt tatctagagc gttatgtcta
     4801 ccaatgtctt gtcgatgtac aaaaaatgtc aaaccatcgc ttatagcagc attatgtaag
     4861 ccacctgttt cttggtaaat atgacttgca ctttgtaatc gagtcatcat gttaataatt
     4921 tgcattggag ttaaagtgat tttagacata gatgttttag cgatagcagc atcattttga
     4981 aaataaaact cacgactctt tccgcaacaa gatgcaatca ttcgttttgt ggaatattga
     5041 aagcgatcgc ctaaatcttt attaagttca acatgggcaa aacctttact atcatcaatc
     5101 agtacagatt ttaattcatc tcgctttaaa atggcacctt ccgaagccag aaatccaatg
     5161 actaactcct caaggtttgt tggactgcat ataacagtcg caaattcttc accattcacc
     5221 ataattgtaa gtggaaattc tgtcacatat tgatctgttg tattgaataa ttttccatct
     5281 tcatatctaa caattggttg acctaaagat acatctttgt tcattatcta acccctttaa
     5341 ttagcttaaa ctttatttta aagaaatttg cttaaaattt taacatattt gcttaagttt
     5401 gaaatttgat tgataaaaat taatagcgag caatctgttt gatttaaatt gaattcgaga
     5461 atatacatac tagggcatca attaataaat atcaatctta tgcaaatttg acaattgttt
     5521 gaatcaatat ataaacaggc aacggttctt ttcaaatata atagtaagtg tataatgaaa
     5581 atgtaaatat tattaaaaat gggggttcac tcaatgaaat tgaaacgttt atttgctgtt
     5641 gtgattgcaa tgcttttagt attagctggt tgctctaatt ctaacgataa taatgaaagt
     5701 aaaaaagatg acgcagacaa tggtaagaaa caagagattc aagttgcagc ggcagcaagt
     5761 ttaacagatg taaccaagaa attagcttca gaatttaaaa aagagcataa aaatgctgat
     5821 attaaattta actatggtgg atcaggggca ttaagaaaac aaattgaatc aggcgcacct
     5881 gttgacgtat ttatgtctgc aaatactaaa gatgtagatg cattaaaaga caagaataaa
     5941 gcgcatgata catataaata tgcgaaaaat agtctagtat taattggtga taaagattca
     6001 aattacactt cagtaaaaga cttaaaagac aatgataaat tagcattagg tgaagtgaaa
     6061 actgtaccag caggaaaata tgcgaaacag tatttagata acaataactt atttaaagaa
     6121 gtcgaaagta aaatcgttta tgctaaagat gtaaaacaag tattaaatta tgttgaaaag
     6181 ggtaatgcga aacaaggttt tgtgtataaa actgacttat ataaacaaaa taaaaaaatt
     6241 gatactgtaa aagtaattaa agaagtagaa cttaagaagc caatcacata cgaagctggt
     6301 gctacatcag atagtaaatt agcaaaagag tggatggaat tcttaaaatc agataaagct
     6361 aaagaaatac taaaagaata ccactttgca gcataaggag ttgtaatcca tgcctgactt
     6421 aacacctttt tggatatcaa tacgagttgc tgtaatcagt acgattattg taacggtttt
     6481 aggtattttt atatctaaat ggttgtatcg tcgtaagggt tcgtgggtta aattattgga
     6541 aagtttattg atattaccta ttgttttgcc gccaacggta ttaggtttta ttctattaat
     6601 catcttctcg ccaagaggac caatcggtca attctttgcg aatgtactac atttacctgt
     6661 agtgttcact ttgacaggtg ctgtgatagc atctgtcatt gttagttttc cactaatgta
     6721 tcaacatact gtgcaaggct tcagaggtat agacacgaaa atgattaata cagctagaac
     6781 gatgggagca agtgaaacga aaattttcct caaattaatt ttaccattag ctaaacgctc
     6841 tattttagca ggtataatga tgagttttgc tcgtgcatta ggtgagtttg gtgctacatt
     6901 aatggttgca ggatatattc caaataaaac gaatacacta cctttagaaa tatacttctt
     6961 agtggaacaa ggtagagaaa atgaagcgtg gttatgggta ttagtgctag tcgcattctc
     7021 tattgtggtt atatctacaa ttaatttatt gaataaagat aaatataagg aggtcgacta
     7081 gatgcttaaa atcaatgtga aatatcaatt aaagaacact ttaattcgca tcaatataga
     7141 tgatactgaa ccaaaaattt atgcagttcg tggtccatct ggcattggta aaactactgt
     7201 tttaaatatg attgccggat tacgtaaagc agatgaagct attatcgaag tgaatgggca
     7261 attacttact gatacggcaa aaaacgtgaa tgttaaaatt caacaacgac gtattggata
     7321 tctgtttcaa gactaccaat tgtttcctaa tatgacggtc tataaaaata ttacttttat
     7381 ggctgaacca tctgaacaca tcgatcaatt aattcaaact ttaaacattg atcatttgat
     7441 gaaacaatat cctatgacat tgtcaggtgg agaggcacaa cgtgtagcac ttgcacgtgc
     7501 acttagcacg aaaccagatt taattttatt agatgaacct ttttctagtt tggatgatac
     7561 tacaaaagat gagagtatta cattagttaa acgtattttc aacgaatggc aaataccaat
     7621 catatttgtg acacattcaa actatgaagc agaacaaatg gctcatgaaa ttattacaat
     7681 tgggtaatca tttatttgcc attaaagagt ttagaacgta tttaaaattg tagaagtgaa
     7741 tgcttctatc agcattttaa tgatgtttta aactcttttt taggggcagt ttttttgaga
     7801 gacattgacg cgcgtcatat aatgaaagta atgataaaaa gaaaggataa cttaatgtga
     7861 gtcaagaacg ttattcaagg caaattttat ttaaacaaat aggtgaaata ggtcaaagca
     7921 aaataaatca aaaatgtgcg ttgattattg gtatgggcgc attaggtaca catgtggccg
     7981 aaggacttgt tagagcaggc attgccaaac taatcattgt tgatagagat tatattgaat
     8041 ttagtaattt acaaagacaa acattgttta ctgaagaaga tgctttgaaa atgatgccta
     8101 aggtggttgc agctaaaaag catttgctag cgttacgtag tgatgttgat attgatgatt
     8161 atattgccca tgtggattat tattttttgg aaacacatgg acaggacgtt gacgttatta
     8221 ttgatgcaac cgataacttt gaaacacgac aactgattaa tgattttgca tataaatatc
     8281 gtataccttg gatttatggt ggtgttgtac agagtacata tacagaagct gcatttatac
     8341 ctggtaaaac accttgcttt aactgtttgg taccacaatt gccagcatta aatttaacat
     8401 gtgatacagt aggggtcatt caacctgccg tgacgatggc aacaagttta caattaagag
     8461 atgcgatgaa agtattaacg gaacaaccaa ttgacacaaa aataacttat ggcgatattt
     8521 gggaaggtag tcattattca tttggtttca gtaaaatgca acgttcagac tgtacaactt
     8581 gtggagatgt accaagttat ccgtatttaa acaagaatga acaacgttat gcaacattgt
     8641 gtggtagaga cactgtacag tatgaaaatg catcaattac acacgacatt cttgttcaat
     8701 ttttaaaaca acatcagtta aattatcgca gtaattcgta tatggttatg tttgaattta
     8761 aaggacaccg cattgttgct tttaaaggtg gaaggttttt aatacatggc atgacacgca
     8821 catcagatgc cacacatcta atgaatttat tgtttggata aaaaaagata agacaaaagg
     8881 agtgtaatat tatgggcgaa catcaaaacg ttaaattgaa tcgtacagtt aaagcagccg
     8941 tactaacggt atcagatact agagactttg atacagataa aggtggtcaa tgcgtgcgcc
     9001 aactattaca agcagatgac gttgaagtga gtgacgcaca ttatacaatt gtgaaagatg
     9061 aaaaagtagc catcacgacg caggtgaaga agtggttaga agaagatatt gatgtcatca
     9121 ttacgactgg tggaacaggt attgcacaac gtgatgtgac gattgaagca gtaaaaccac
     9181 ttttaactaa agagatagat ggctttgggg aattgtttag atatttgagt tatgttgaag
     9241 atgttggcac gcgtgcatta ttgtctcgtg ctgtagcagg tacagttaat aataaattga
     9301 tattttcgat tccaggatca acaggcgcag ttaaattagc attagaaaag ctcattaaac
     9361 cagaattaaa tcatctgatt catgagctta caaaataatt tattgatttg attggcgttg
     9421 aaaatctcca gatttaccgc cagacttgct ttcaaggtag gtttcgccaa taatcatacc
     9481 tttatcaact gctttcgtca tgtcgtaaat ggttaaagcc gttgctgatg cagcggttaa
     9541 agcttccatt tcaacaccgg ttttgccagt tgtagagaca gttgtttgaa tgtttaaagt
     9601 ataaaggggt gcatttgttt catcccagct gaagtgaaca tctatgccag tcaatggtaa
     9661 tggatggcac atcggaataa gtgttgatgt atttttggca gccataatac cagcgatttg
     9721 agcagtgttc aatacattgc ctttggtatt ggtgttatta gaaatctgct taaaaatagt
     9781 ttcatttaca gtaatgcttg aatgagcgac agcagttctt tttgtaattt gtttgtctga
     9841 tacatcgacc attttggcgt ggccttgttg attaatatga gtaaactcag tcattttacc
     9901 cctcctagtg catctagtat atcatgaaaa aataaaagtt ttggagatga tttttaatgg
     9961 tagtagaaaa aagaaaccca atcccagtta aagaagcaat tcaacgtatc gttaatcagc
    10021 agagttcaat gccggcaatt acggtagcac ttgaaaaaag tctaaatcat atcttagcag
    10081 aagatattgt agctacttat gatataccaa ggtttgataa atcaccttat gatggttttg
    10141 caattcgcag tgttgattca caaggggcaa gtggtcagaa tcgcattgag tttaaagtga
    10201 ttgatcatat tggtgcaggt tcagtttctg ataaattagt tggggatcac gaagcggtgc
    10261 gtattatgac tggagcacaa atacctaatg gcgcagatgc tgttgttatg tttgaacaaa
    10321 cgattgaact agaagataca tttacaattc gtaaaccatt ttcaaaaaat gaaaatatat
    10381 ctttaaaagg tgaagaaaca aagacaggcg atgttgttct aaaaaaagga caagtaatta
    10441 atccaggggc tatcgcggtc cttgcaacat atggctatgc agaggttaaa gttattaagc
    10501 aaccgagtgt cgctgttatt gcaacaggaa gcgaattatt agatgttaat gatgtattag
    10561 aagatggaaa aattcgtaac tctaatggcc caatgattcg tgccttagca gaaaaattag
    10621 gtcttgaagt tggtatttac aaaacacaaa aagatgattt agatagtggc atccaagtcg
    10681 ttaaagaagc tatggaaaaa catgatatcg ttattacaac gggcggagtt tctgttggag
    10741 attttgacta tttacctgag atttataagg ctgtaaaggc ggaagtgtta tttaataaag
    10801 tagcaatgcg tcctggtagc gtaacaacgg ttgcatttgt agatggaaag tatttgtttg
    10861 gattatctgg aaatccatca gcttgtttta caggatttga actatttgtg aaaccagctg
    10921 ttaaacatat gtgtggcgca ctagaagtct tcccgcaaat aattaaagca acattaatgg
    10981 aagattttac caaggcaaac ccattcacac gatttatacg tgctaaagca acgttaacaa
    11041 gtgctggagc tactgtagta ccttcaggat tcaataaatc aggtgcggtt gtagcgattg
    11101 cacatgctaa ctgtatggtc atgttaccag gagggtcacg tggttttaaa gcggggcata
    11161 cagtagatat tatattgact gaatctgacg ctgctgaaga ggaacttctt ttatgatttt
    11221 acaaattgta ggttacaaaa agtctggtaa gacaacattg atgaggcata ttgtctcttt
    11281 cttaaagtca catggttata cagttgctac tattaaacat catgggcatg gtaaggaaga
    11341 tattcaatta caggattcag acgtcgatca catgaagcat tttgaagcgg gggcagatca
    11401 aagtattgta caaggttttc aatatcagca aactgtaaca cgtgtagata atcaaaatct
    11461 tactcaaatt attgaaaaat ctgttacaat tgacaccaat atcgtattag ttgaaggctt
    11521 taaaaatgct gattttgaaa aagtcgtagt ctatcgaaat gaagaagagt tgcaagtatt
    11581 acaacaattg tcgaatgttt gttatagcat taatgtaagg gagcatgaag attttacagc
    11641 atttgagcaa tggttattaa ataaaattaa aaatgattgt gatacacaat taacatagag
    11701 gattgaaatg aatgaaacaa tttgaaatcg tgacagaacc gatacaaaca gaacaatatc
    11761 gtgaattcac tataaatgaa tatcaaggtg cagtagttgt ttttaccggt catgttcgcg
    11821 aatggactaa aggcgtcaaa acggaatatt tagaatatga agcgtatatt ccaatggctg
    11881 aaaagaaatt ggcacaaatt ggagatgaaa taaatgaaaa atggcctgga acgataacga
    11941 gtattgttca tagaataggg ccattacaaa tttcagatat cgctgtatta attgcggttt
    12001 cttcaccgca tcgtaaagat gcctatcgag caaatgaata tgcaattgag cgtataaaag
    12061 aaattgttcc gatttggaaa aaagaaattt gggaagatgg ttcaaaatgg caagggcatc
    12121 aaaaagggaa ttatgaagaa gcgaagaggg aggaataaga gagatgaagg tactttactt
    12181 cgcagaaatt aaagatatat tacaaaaagc acaggaagat attgtgcttg aacaagcatt
    12241 gactgtacaa caatttgaag atttattgtt tgaacgttat ccgcaaatca ataataaaaa
    12301 gtttcaagtt gctgtaaatg aggaatttgt acaaaaatcg gatttcattc aacctaatga
    12361 tactgttgca ttaattccac cggttagtgg aggttaaggg agcatgaaag caataattct
    12421 tgcaggtggt cattcagtgc gatttggtaa gcccaaagct tttgcggaag tgaacggtga
    12481 gaccttttat agtagagtaa ttaagacatt agaatcaaca aatatgttca atgaaattat
    12541 tattagtaca aatgcgcaat tggcaacgca atttaaatat ccaaatgttg ttatagatga
    12601 tgagaatcat aatgataaag gtccattagc aggaatttat acaatcatga agcaacatcc
    12661 tgaagaagaa ttgttttttg tcgtttctgt tgatacacca atgattactg gtaaagctgt
    12721 aagcacgttg tatcagtttt tagtttctca tcttattgaa aatcatttag atgtcgcagc
    12781 ttttaaagaa gatggacgtt ttattccaac aattgcattt tatagtccga atgcattagg
    12841 cgctataact aaagcactac attctgataa ttacagtttt aaaaatgtat atcatgaatt
    12901 atcaacggat tatttggatg taagggatgt agatgcgccc tcatattggt acaaaaatat
    12961 aaattatcag catgatttgg acgctttaat tcaaaaattg taagctgtta ggaggtccac
    13021 aaatggtaga acaaataaaa gataaactag gacgtcccat ccgtgactta cggttatctg
    13081 tgacagatcg gtgtaacttt aggtgtgatt attgcatgcc taaagaggta tttggagatg
    13141 atttcgtatt tttacctaaa aatgaacttt taacgtttga tgaaatggct agaatcgcta
    13201 aggtatatgc agaattaggt gtaaaaaaaa tacgcattac aggtggagaa ccattgatgc
    13261 gacgggattt agatgtactt atagctaaat taaatcaaat cgatggtatt gaagatattg
    13321 gtttgactac aaatggtttg ttattaaaaa agcatggaca aaagttatat gatgctgggc
    13381 tgcgcagaat taatgtcagt ttggatgcta ttgatgatac gctatttcaa tcaatcaata
    13441 atcgtaatat taaagcgact acgattttag aacaaattga ttacgcgacg tctattggtt
    13501 tgaatgtaaa agtaaatgtt gttatacaaa aaggtattaa cgatgatcaa atcataccaa
    13561 tgcttgaata ttttaaagat aaacatatag agattcgatt tatagaattt atggatgttg
    13621 gtaatgataa tggatgggat ttcagtaaag ttgtaactaa agatgaaatg cttacaatga
    13681 tagagcagca ctttgaaatc gatcctgtag aaccaaaata ttttggggaa gtagcaaaat
    13741 attatcgcca taaggataat ggtgttcaat ttggtttgat tacaagtgtt tcacaatcat
    13801 tttgttctac atgtacacgc gcaaggctgt catcagatgg gaagttttac ggatgtttat
    13861 ttgcaactgt cgatggattt aacgttaaag cgtttattcg ttctggcgtg accgacgaag
    13921 aattaaaaga acaatttaaa gctttatggc aaataagaga tgatcgatat tcagatgaga
    13981 gaactgctca aacagttgcc aatcgtcaac gtaaaaagat aaacatgaat tatattggtg
    14041 gttaatgtgt agggaccact acatattaaa tcattagaga tgttttaata tttctgtctt
    14101 actccctaaa atacaatatt atttattaaa gtaaaaacgg tcatatctat gccagattta
    14161 atagaaatga tcgtttttaa agtttttaca agttggcggg gccccaacac agaagctgac
    14221 agaaagtcag cttacaataa tgtgcaagt
//
LOCUS       DANRDI010000061        14095 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.061, whole genome shotgun sequence.
ACCESSION   DANRDI010000061 DANRDI010000000
VERSION     DANRDI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.061"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..554
                     /locus_tag="PZ377_001409"
     CDS             <1..554
                     /locus_tag="PZ377_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDM6358283.1"
                     /translation="FFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVR
                     NISESFNFNIVDLKITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVM
                     ILFAVLILFRGLGKDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVP
                     IVGAFLIDLIGVIVIMGFIQWFS"
     gene            complement(602..1651)
                     /locus_tag="PZ377_001410"
     CDS             complement(602..1651)
                     /locus_tag="PZ377_001410"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDM6358284.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(1682..2629)
                     /gene="corA"
                     /locus_tag="PZ377_001411"
     CDS             complement(1682..2629)
                     /gene="corA"
                     /locus_tag="PZ377_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDM6358285.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            2726..3217
                     /locus_tag="PZ377_001412"
     CDS             2726..3217
                     /locus_tag="PZ377_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDM6358286.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            3214..3633
                     /locus_tag="PZ377_001413"
     CDS             3214..3633
                     /locus_tag="PZ377_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358287.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(3741..4661)
                     /locus_tag="PZ377_001414"
     CDS             complement(3741..4661)
                     /locus_tag="PZ377_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6358288.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            4869..5507
                     /locus_tag="PZ377_001415"
     CDS             4869..5507
                     /locus_tag="PZ377_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358289.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(5753..7684)
                     /locus_tag="PZ377_001416"
     CDS             complement(5753..7684)
                     /locus_tag="PZ377_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDM6358290.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(7697..8344)
                     /locus_tag="PZ377_001417"
     CDS             complement(7697..8344)
                     /locus_tag="PZ377_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDM6358291.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            8465..9016
                     /locus_tag="PZ377_001418"
     CDS             8465..9016
                     /locus_tag="PZ377_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDM6358292.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            complement(9246..10454)
                     /locus_tag="PZ377_001419"
     CDS             complement(9246..10454)
                     /locus_tag="PZ377_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDM6358293.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(10564..10692)
                     /locus_tag="PZ377_001420"
     CDS             complement(10564..10692)
                     /locus_tag="PZ377_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358294.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            complement(10723..12105)
                     /locus_tag="PZ377_001421"
     CDS             complement(10723..12105)
                     /locus_tag="PZ377_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358295.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(12345..12800)
                     /locus_tag="PZ377_001422"
     CDS             complement(12345..12800)
                     /locus_tag="PZ377_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDM6358296.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            12995..13963
                     /locus_tag="PZ377_001423"
     CDS             12995..13963
                     /locus_tag="PZ377_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDM6358297.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYTAFIYNS"
BASE COUNT         4612 a         2488 c         1942 g         5053 t
ORIGIN      
        1 tattcttcat tcaatttaca atcgttgtat tctgtatggc agttggaagt tatttcagtc
       61 atttgtttac agctcaaaca gggattaatg ttccaattta cgttggctca ttatttgtag
      121 ctgttattgt ccgaaatatc tctgaaagtt ttaattttaa tattgtagat ttaaaaatta
      181 ctaatcaaat tggcgatgtc gcattaggta ttttcttatc tcttgcgcta atgagcattc
      241 aattaatcga aatttataaa cttgctatac ctcttattat tatcgtttta gttcaagttg
      301 tcgttatgat tttatttgct gttttaattt tatttagagg tttaggaaaa gattatgatg
      361 ctgcagtaat ggtaggtggt tttatcggtc atgggcttgg tgcaacgcca aatgccatgg
      421 caaatttaga tgttattact aaaaaatatg gaaactcacc taaagcatat ttagttgtac
      481 ctattgttgg tgcattctta atcgatttaa ttggtgttat agtcattatg ggattcatac
      541 aatggtttag ttaaacacca aactcataaa taaaagagga ggccttcgcc tcctctttta
      601 tttatcctcg atgtatattc aagttacgtt gttctatcca tgacaatatt tccggactaa
      661 atacgatttg tttttgtgtt aagtcgtcaa tatttttagc atctaacatc gtcattattg
      721 atttcatgtg ttcaataaat gattctacat aagctactgt atgtgcaatg ccattatttt
      781 caacttgatt taaaaacgga cgtgacatac cagttgcctt tgcaccaagt gctaaacttt
      841 taattgcatc gagtggtgta cgtaaaccac cactcgcgaa aactgaaatt tcgctttgat
      901 aagccgttgt ttcaagtaat gactcaactg tagactgtcc ccatgatgat aagtaatcca
      961 tatctttatt tgcacgacgt tcattttcaa tatctacaaa gttagtacca cctttgccac
     1021 taacatcgac atacttgacg cctatttgtt gtaagtcatg cattaattct ttgctcatac
     1081 caaatccaac ttcttttata atgactggaa cagacactcg tgatacaatc gacgctatat
     1141 tatctaacca agtcacaaat tcacgattcc cttcaggcat aactaattct tgaggagaat
     1201 taacatggat ttgtaacgct tgtgcctcaa gtaattcaac tgcttccaaa gccttttcta
     1261 ctggtacgtc cgcaccaaca ttgctaaaaa tcatgccttc aggattcatt tttcgcgcaa
     1321 tcgtaaacgt ctcagccatg cgtggatttc tcaatgccgc atgtgttgat ccaactgcca
     1381 tcgctaagcc agtttctctt gcaactacag ctagcttttc attgatgttt ttcgtccact
     1441 cgctaccacc cgtcattgca ttaatataaa ccggatatgc catcgttaag tcaggcgtct
     1501 gtgatgtcaa atcgatatca tttacattaa ttgatgggat agaatgatgc acaaaacgca
     1561 tcttatcaaa atctgaatgc attgcgtcag attgggccat tgctatttca acatgttcat
     1621 tttttctctg ttctctttga aaatcactca tgattaaacc taccttttcg tcatttctaa
     1681 tttaaaacca ttttttacgt ctgaaaaaga tgattaatac gacagctata acaaacatta
     1741 cagctaagca aatataatat ccataatgta aatgtaatgc cggcatattt acaaagttca
     1801 taccataaat cccagctatg aatgttaacg gtgaaaatat aactgatact aatgtcagta
     1861 cttgcataat actattcatt ctaaatgacg tgtatgactc aaaattttct cgtatttcgt
     1921 ttgtcatttc ttgagcagta cgaatgatat tacgttgctt aatcaagtgg tcatcgatat
     1981 gttgaatgta tagcgaatgt ttattatcta taatcaaatc accattttgt ttcattgtat
     2041 caattagctc ttgcatagga aacagtacac gttttacttt aatcaaatcc gaacgtaact
     2101 taaagacact atccatgacc attttattaa agcgatcatc tacatggcgg tcttcaaaat
     2161 gataaacact atcttcaagt gcatatacaa agttgaaata tttatcaacc atcatatcta
     2221 aaattaatat gacgacatct gcacaatcta attctgcatc taatgtattc atatacttat
     2281 agactacttt atttaatgat tccaacgttt gatgatgata tgttactaat acattgtctt
     2341 gtataaaaat atttagtgct attggtgaat agtttgaccc cataatacta tggaatacta
     2401 agtattgata atctttataa gatttatatt tagctcgtgg cataccgtta attgcatcat
     2461 ccacttctaa atcattaaaa ttaaaatgtg ctttaaacca ttcattttct tgttcattcg
     2521 gttcatcaaa atcataccaa acaatagtcg catcttttgg tatctctttg atatcatcaa
     2581 ctactttaaa cggttcatat gtagtttgat accgtatctt taaagccatc gatactcccc
     2641 ctaaataacg aattctctat tattttatca tgaattaaat aacgtgtatg tcttaattta
     2701 ttttagtatg atagtcacta aggagatggt tattatcaaa caacttttta cacatactca
     2761 aaccgtaaca tctgaattca ttgaccataa caatcatatg catgatgcaa attataatat
     2821 catttttagt gacgtcgtga atcgttttaa ttacagccac ggtctttctt taaaagaacg
     2881 cgaaaattta gcatatacgc tatttacact agaagaacat acgacatacc tctcagaatt
     2941 gtctcttggc gatgtattta ctgttacttt atatatttat gattacgatt ataagcggtt
     3001 gcatttattt ttaacattaa ctaaagaaga tggtacacta gcatcaacaa atgaagtaat
     3061 gatgatggga attaatcagc acacacgtcg ttctgatgct tttcctgaat cattttcaac
     3121 acaaatagca cactattata aaaatcaatc aactatcact tggcctgaac aattaggaca
     3181 taaaatagca attccacaca aaggagcatt aaaatgacag atgcattaca acaaaagatt
     3241 catatcgaat tactagattt attagatgat gttaagtttg aattaacaga attaaatgca
     3301 caaaaagggt tatacattaa cggaccagca aatcagctac ttaagcgtgg cgtgcatatg
     3361 gcttatgttc aaggacaaaa gcaagccatc gataatatta tgactattgt ggaacaacag
     3421 cttgaagatc aacatttcct agaacattat gataaatttc aaaatgaggt tgctcatcgc
     3481 aattatgata aaacagctaa ctttgcagaa cttagtgata ttccacgtca attcgataat
     3541 tttttagatc aattttatca aattaaaggg caatacttta tcatcacaca tatcaataca
     3601 cttattggtg attttcactc agaagctcat taacaattag tctatataac ccttgctata
     3661 ttttcaaaaa caaaacccaa ttacgttttc atgtcaaata tcatcttgca tgaaatcgta
     3721 actgggtcat ttatatgtta ttagttattt tgtgttacat cctcatctat cgatttggca
     3781 atttgtttaa tagctttatg tgattgtcta attggataaa ttggaaaatc atgtaccatc
     3841 ttaggataat cataaaactc aatgtattga tgatgttgca acatcatttg ttcaaatagc
     3901 ttcatatcag gatgtgtcat ttcacgtcca ccaccaaaca tataaactgg tggcaatcct
     3961 tctattgtgc cattaattgg cgatatgcgc ttatctgtta atggtaggcc attcgcccat
     4021 tttttcataa tctcattgac accaaactga cttagaaccg catcttgttc gattaaggcg
     4081 tccgaaatat ctttattaga tagtgttgca tctaaaattg gtgagattaa atacaattta
     4141 ttcggtaatg gctgttgatt atctaaaaga gattgtacaa aggataatgc cagtgcacca
     4201 cctgaaccat cacccatgac tacgacattt tgatgtccta cttcagatac taattgatca
     4261 taaacacgtt gtatcgcttg gaaagtatcg tcaatatgaa actctggtgt ctttggatag
     4321 ataggcagta caacctcata taatgtactt aaagtgattt tatccaacaa tctccaatgg
     4381 aacggtgatg gttgtagtgc attgaatcca ccgtgaatat ataaaatttt cttatcaatt
     4441 tgatgtctga aattaaagcg aaagacttgc atatcatcta atgacaattt ttctaaattt
     4501 gctttaacat ttaatgttga aggctgctta tgtttttttc tattttcaat ttctctttta
     4561 taaaaaaatc tttcaacatc ttgatcattt ttaaacataa tcgagcgatt gtgaagcaaa
     4621 tatttattga caacgctatt cataacacgg tttctaatca atgtcttaac ctacctttat
     4681 atattttatg tatccaatga ttgtctatcc cctacattct ttgccaaaaa aagtatataa
     4741 tgtagaagat attttctttt tcactttcaa atttaagact acaattgaac agtgattttt
     4801 catcattata acagacaact agacatattg ataagtaaag aaaagaactt tatacggagg
     4861 taccttgcat gacaaatcca aatcaacgat tagaaccatt tgatgagaca tttcaacaac
     4921 cgaatattca tcgtggtaag cgatatggta agaaaaaacg ttcattggta agcatgatta
     4981 ttcaaatcat tgttgttata ttaaccgcaa tcgctggata cagcatgtgg aaacaaccca
     5041 tttttaatat tgtcttcacc aaagaatctg tagattttga tggcattaaa aattttaacg
     5101 agacaattgg aaaaatgcat caattaaata ttaatttggg taatatcaat gatttacaac
     5161 aaggaatcga tcgcttaatt ttagtcttta acggtttctt tattttatgt atcgttagcc
     5221 tagtcataac aatactgaca attgtattta atcgcacagt tctaaaagta gtaaatattt
     5281 tagtgttaac aattatgtta gtcattacat tgtatttcag ttatattatt aaagcactcg
     5341 cacaagaaat tgcagatgtt ttcaaaaaat attatttaaa tgtaccacca gatcaagtga
     5401 ttacagaagc tgacgctatt cataacggca tcattttact tggctgtagc atcggtttac
     5461 ttgttataag cttattcttc cgtaatcgca tgcctcgttt aaaataatgt tatatagtag
     5521 ggattgggac agaaatgtta ttttcacaaa attcatttcg ttgacccact tcctgctttt
     5581 tctttattaa tgacctacta cgtcaatgcc aaattttaat ataattcctc taacattcta
     5641 taaatcaaca aataaaaaaa gtatcgataa ccatatttgg caccgatact ttaaaaatca
     5701 tgtccccaca acataatttt tgattagtca cacttcaatt aatttatttt atttattcat
     5761 gattgatact attatctgca tctaactttt gagctgtagc ttttgctttt ttattacttt
     5821 gtaaaaataa acataaaatt aaggcgatga tgctaaatat cgttgcaact ataaatgcat
     5881 cattaatacc ttcaaccgtt gctagtttat ttacaaattg tagtaacact ttcattgcgc
     5941 cttcttgtcc gccatattgt gatgctaatt catgcatatg atcttgtaca acaggattcg
     6001 ttttatctaa ctcttcccca aaagctgata agtgttgtgt agtttgtgtt gtcattacag
     6061 taactaagat tgctgtacct atagagcctg ctaattgacg catcgtattt aagaaagcat
     6121 taccatgaga ggcaagtcgt cccggtaacg cattaatagc tgcagttacc attggcatca
     6181 ttataaatgc cataccaaat gaacgaagta catagatacc catgattgtc atatatggtg
     6241 tatccatatt taatttagtt aattcccatg ttgcataagt cattacagca ataccaaaga
     6301 tagctaatgg ttttaaacca atagtatcta acaatttacc tgcaaatggt cctagtagac
     6361 ccataattag agaaccaggt aataataaca atccggaatc taatgctgag aatccgcgta
     6421 aattttgtaa ataaatcggt aataaaatca taccaccata taaacttaac attacaacca
     6481 tattaataat tgttgttaat gtaaatgttg ggaatttcaa tacttctaaa ttcaacattg
     6541 gtgatttcat tcttaattct ctaataacga atagaataat aaagataata ccaatcgcaa
     6601 acattgtttc tatctctact gaaccccaac ctttgttgcc agcttctgag aaaccatata
     6661 acaaagcacc aaaaccaatc gtactaaaaa tgatacctgg gatatcagct ttagggtttg
     6721 ttgtatattg atataactta aaccatacaa aaccaattaa aatagcgata atcccgataa
     6781 tgaacatacc gtaaaacatc acattccaat ggtaattttg tacaatataa cctgataatg
     6841 ttggaccaat tgcaggtgct aaaatcattg cgatacccat tgtacccatg gcagcaccac
     6901 gtttttcagg tggataaatt gtaataataa caattgaacc taatggcatt agtacacctg
     6961 caccaatggc ttgtaataca cgtccaacca tcatgattgg gaaattcatt gaaatcgcac
     7021 agattaatga accaattgta aagagtacta acgcaactaa aaataatttt cgatatgaat
     7081 atttattaaa tagatacgcc gtaattggta ttaaaatacc gtttactaac atgaatcccg
     7141 tcatcaacca ttgccctgtt gacgcagaaa tattaaattc cgtattaatt tttggtaaag
     7201 caacatttaa taatgtttgg tttaaaatcg caataaacat accgaataat aatgccgcta
     7261 atattttacc gcgtgaaaca ccttcaccaa aaataaagtt tttatgttct ttttttattt
     7321 tttcattcac tttatattct tctgattcag gatttttagc agcaactgct tcctcatcct
     7381 tattattagt gaatgcttct tgatctttct cagacccctt tgtgtaacca tttagactaa
     7441 cttggctatg atcatcttga ttgtcaacac gcaactcttc atgcgtcata cgttgtgtcg
     7501 attgatcagt tgttttgtct aaatcactag ctttaaattt agattgattt gattgacgtg
     7561 tcgtaaattg ttgttccttt tgttggcgtt tgcctttttt tcttgatctt attaaaaata
     7621 aattgataac cccaacaata atgagcgcta aaataatgta gctaataatg aaggtcgtag
     7681 tcatttaatg acccccttaa tttttatgga tttttacttc agcgttcatt ccaggaacaa
     7741 cttgtttaga cggttctgat tctagagtga ttttaacagg tattacttga gaaactttag
     7801 tgtagttacc atcactattt gatgatggca ttaatgaaaa gcttgcagca gttgcttttc
     7861 caatactatc aactttacct ttaatagaag ctttttgacc gtcaatagtc acatcaacat
     7921 ctttacctac ttcaacatct ttaatatctt tttcgtcaat atttgctgtt acatataaat
     7981 catctaaatt gtatgcataa gcgattgggt taccagcttg caccattgaa ccttccatac
     8041 catctaattt ggcaattgta cctttttgag gcattttaag atccatatct ttcgtttcgc
     8101 catcttgacc ttgtacagta acaattgcta ctttgtcacc tttatcgagt ttgtcacctt
     8161 gtttaacatt aagtgattta atttgtccag atgcaggact tgctatttta atttgatcgc
     8221 catttacttt tgcattatca gttgttacat agcttgttgt tttattccaa aaataaaagc
     8281 cagcaatccc aattgctaac agtacaacaa ccgtaatgac attaattaat atcatctttt
     8341 tcatgaaaca tttcctccta tttgctttta aaattcactt tatttattat agagacatga
     8401 taactaaaaa aacaccaaca atttttataa gagtgttggt taaaattcac aaaggtgtaa
     8461 caatatgaaa cgacaagcca aaatagaaat tcaaaacgca cttgttgatt tgatggctga
     8521 atatcctttt caagaaatat ccactaaaat gatttgcgcg tattgtaata ttaatcgttc
     8581 tactttttat gattattaca aagataaatt tgatttattg gataccatca actcaaagca
     8641 taaggaaaaa ttccaatttc ttctaagcgc gctacatcat aatttcgaaa atattaaaca
     8701 ggataagctt aaactttata aattcttcat tattattgct aagtatatca agcacaatga
     8761 gcagtttttc aaagacattt tagttaccta tccaatgaaa acattattca tagactatat
     8821 aaatttagcg agagattact atcaacaaat tatgaatgac tatagtacta cagtaaccaa
     8881 taaacagctt tatgttacat atatcatagg cggtcaagct ggggtgttta ttaattggct
     8941 ttcgaatggt tgtaatgaat cacctgaaga agttgctgac attttattgg ctaatacgat
     9001 taaacttcaa aactaataat acggtttcat tctaataaag aaatgctata aaacaactag
     9061 ataaagttaa accaaatgga tagataatag tacatatgta acggtgctat caatataaaa
     9121 atgccgagat ttccacataa taatctcggc atttttattt ttgatgctaa attcataggt
     9181 ctatgaatag ctcaagtttt gttttttaat ttttcaattc aatataatag aagaaactat
     9241 gtttatcatc gatttgtttt aaataccttc atattataaa tctgtaaaat aattaatata
     9301 actgctgtag ctataataat aattatgtaa ggtataggat tatcttcacc ttttaccccc
     9361 actaatggcg atgcaacacc accaaataaa aactgtacta aacctaataa acttgacgaa
     9421 ctccctctac cactactact ttcatccata gctattgtaa aaccaagtgt cgcaacacct
     9481 gtcactggtg ctattaaaat gataaatcct atcgctaaaa tccaaaagtt ccaatgatta
     9541 agcaatacaa tcgtcactaa aattacaccg ataatctgaa tcatcgtcat gcctctcatc
     9601 aatttttgtg agtctataaa atcaacaagg tacccagtaa gttgacttga tataatgagc
     9661 gtaataccaa ttcctgcaaa catccaacta aattgtattg cagtcatgcc ataaattttt
     9721 tgaataataa acggagacgc agaaatataa gtaaataata ttacaaatgt cataccttga
     9781 atgagcattg gtaatacaaa acgtggcgtc ttcaataata ttttgaagtt tttaaacatt
     9841 gtctttaatc cactacttga ctcacgattt gtcactgtta atgattcagg tacttttaat
     9901 aaagaaccta tgaccatgac gaagccaaag atagtcagaa tgacaaagac catacgccag
     9961 acagaataat ttaaaattat gccccctatt gttggcgcaa caactggtgc aataccatta
    10021 acaagcatca ataatgccat aaacttagtt agttcattac cactatacat atcacttgct
    10081 atagctcttg aaataactgc tgctgcgcca cctgtcactc cttgaagaaa tcttaatgca
    10141 accatcagcc aaatattatg tacaaaaaca atacctaaac ttgctaatgt aaaaataatc
    10201 atagctataa taagcggctt tctgcgccca gttgaatctg aaattggacc agcaaacaaa
    10261 ttaccaaacg ctaaaccaat cataaacatc gataatgtta attgtgcgtt agacgtcgtt
    10321 gtttgaaaat catgtctaat atctggtaat ccaggcaaaa acatatctat cgataatgct
    10381 ccaatggccg tcaaagatcc taaaataata ataaatatcg gtgaacgctt gttaaaatgc
    10441 gttaaatcat tcatatgact atctccttta tataatgatt agtttataac tatcacgaac
    10501 agaaactaaa tttaaatatg ctcttttata tattagcatt agtcacaagt ttcatgaaca
    10561 tcattacaac ttataaaagg tataaaatga tataaacctc agcacttcat aaatttttcc
    10621 acaaaaaaca atcccttcaa agtaattcac actaattgta aatactttga aaggatgttg
    10681 tttgtattcc atgacacatg catcttattt aagttttata ctctattttt ctgatgtctt
    10741 gattaattgc aagcggtctt gcgagccttg ttcaagctca tattctttat ctacttgctt
    10801 accgttttca tttattaatc ttacggttgc ggtaatctta tcaccatgtt tttcagcatc
    10861 tataatttgt ggtgaactaa tcatcattaa acttccttta gatacacgct tctttacatc
    10921 atcataaaaa gatgatcctt tttttatata tgatgataca aaatcaaaat ccgactgatt
    10981 aaacgcagca ttattcgcta aagaatatcc agcaaagaat tctatcaatt tgttcttcaa
    11041 gctgttttct tctttttctt tcttttcaac atagtcttcg atatcttcac tgtcaaaatt
    11101 caaagttatc tctgtattgt attttaaatc gctagcttta atcgtcttcg tctgtgttgt
    11161 aaatgtttta tctttcgctt tacctgaagc tgaaatggta atatctttat tttgtggata
    11221 tggaccatac gttttggaac ttgaaaatgc catttcatgg tcatttatag ttactttttt
    11281 agaactatca tttaattttg tatcgccttt taaagttaca gatatatttg cttcttcaaa
    11341 atcttcagta acatctaccg tttcagaatt actttgtcta aaatcaaatt ttaaatggcc
    11401 tgcaaaatca ccgttctcag ttgatttcat agctggaatt ctatatgtcc ccggtataaa
    11461 attacctatt ggcgtcactt tatttgcttc agctataacc atctttttct taccaccaga
    11521 tttaaactca tattttgttt tttctttcgg tttaacaatt ggttgcttgg taggtgcagt
    11581 aaagctcata ttatcgaaaa agatataacg tgtgccattt ttacttacac gtaatatatt
    11641 ttgaccagat ctggtttgaa tataagaagc tacgctggtc ttactcttat tcaatttatg
    11701 taccgtattt ttaaggtcgc tgacaaattg cttaagccca acttcatctt tgatatagtt
    11761 aatgtatact tttgcttctt cagaatctac tttattatct ttagtactta ataatgttgc
    11821 tactttttgt ttatcattat tctcaatcgc attcactaat attttagttt gcgcttcagg
    11881 tgaattgaag tttcttagta aaaagaacaa tataataatt aagattaaaa tgaaaaaacc
    11941 aattgcccaa ggcaccattt ttctcatctt gatgttatta gtttgtatat cttcatcggt
    12001 tgactttcta tataatgctt gacgactatc aaatttatgt ccacattgtg tacatatctg
    12061 tacatcatct tgtgcttgtt gtccgcactt cgggcaagat ttcatacttt cacctcaatt
    12121 ctgttcctct tcaattgtta tatgtatatc acaaaaatta tttcattatt ttaccattat
    12181 tattttacaa atattatccc aagaatacaa ttcaagaaat gattaacttt actttagaat
    12241 tgtaccttat tacattattt atgaaagtac tctctcaatt ataactaaaa agaaggtgtt
    12301 caatacatac atatttagtc ttatatttga acaccttcaa aaacctattt atgattgcta
    12361 tatgttttaa tgcgatgatt gatgacttct aatacttgcc ttacattttc aatatcttca
    12421 gaatttaaat cattagtaat atcttgcgca atatctgtca taatcgcatt acgttctttc
    12481 aaatatgctc tacctttgtc agttaaggtt atgaatttca aacgttgatc aatatttaaa
    12541 tttggtttat ctaacttaac taatttagca tcgattaact ttttaattcg tcggcttact
    12601 gcggccttat ttacaccttg tctttgcgtg atttcactaa ttgtcaaagg ttctttattt
    12661 aacataccta atacgttaga ctgctctaat gaaatttcat attcattttg taaatctttc
    12721 aacatttttg cagttaatgc gttaacgtta tttataaact gctctaaaaa ttgaatatgg
    12781 tcttgtaaat gttttaccat aattatttct ccctaattac tatttttata aagtatcctc
    12841 agcaatcatt gtatcgcaca tctaaaaaac acaaaaatat tgacccatat ataatataac
    12901 aaattaagat ttacatgttg ctgctttctc cttgctttaa taaattaaat catctacatt
    12961 tatatttaat atttattata agcgaggtgt cagtatgtta ttgaatttat tattaatatt
    13021 tatacttatc gttatttgtc cgttaatttt caactatact ctgacgttaa atttaacatg
    13081 ctacatctta gttgtcatat cgatattttt gtcactcatc tatagcatca tgacctatac
    13141 tttcccttgg tctatgtttg taacatttat aataatcagt attatcatgc tattcaaaca
    13201 tagatattta ttttctcata cttctagtcg cttatttatt aaaaaattat tatctttttc
    13261 atataaattt gtactctaca catcagcact tctatttata agtacgattc agtcacctat
    13321 tgtgaaaggt ttgtctatgt ggttagctat attatgtctt tccctgttac taacctttat
    13381 ttgttatctc gtttggacag tatcatatag acatagatca tacaacaaag aagttgatat
    13441 cataattgtg ttaggtgctg gtatttttac cgaatttgtc acaccaatgc ttgcagcgcg
    13501 tttagatcgt gcacttgata tctatcaaca acaagcatca gcaacaaaga tcattgtatc
    13561 tggcggacaa ggaccagacg aaccaatttc tgaagcttta gctatgcaac gttatttgat
    13621 tgcacatggt gttgataaaa cagatatact aatggaatct cactctataa atacatacac
    13681 aaacttttta tattctaaac aaatcatcaa caatttgtat catgaagcag ttaaaattgt
    13741 ttgtgtaaca agtcagtttc atgtattgcg cgccttacga ctagcccaaa aattaggttt
    13801 atctatcgat ggtgtcggaa gtcatacacc atatcacttt ttcttacatg tgctcatcaa
    13861 agattatcta ggcgtaatgt atcagtatag attgttatta acgttatatt gttcaagtag
    13921 ttggtttata tgtctttaca ccgcttttat ttataatagt taatccacaa acaaactatt
    13981 attataggtt gtttctaata ttgcgtcaaa ctatctttaa atattcgtta atccttgatt
    14041 agacaattgt tatattgaaa ataaataaac taaagagtct gggacattaa tccct
//
LOCUS       DANRDI010000062        14031 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.062, whole genome shotgun sequence.
ACCESSION   DANRDI010000062 DANRDI010000000
VERSION     DANRDI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14031
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.062"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            602..1003
                     /locus_tag="PZ377_001424"
     CDS             602..1003
                     /locus_tag="PZ377_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDM6358298.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(1123..1686)
                     /locus_tag="PZ377_001425"
     CDS             complement(1123..1686)
                     /locus_tag="PZ377_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDM6358299.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1683..2636)
                     /locus_tag="PZ377_001426"
     CDS             complement(1683..2636)
                     /locus_tag="PZ377_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDM6358300.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2746..3927
                     /locus_tag="PZ377_001427"
     CDS             2746..3927
                     /locus_tag="PZ377_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6358301.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            4215..6632
                     /locus_tag="PZ377_001428"
     CDS             4215..6632
                     /locus_tag="PZ377_001428"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDM6358302.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6654..6965
                     /locus_tag="PZ377_001429"
     CDS             6654..6965
                     /locus_tag="PZ377_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDM6358303.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            7291..13851
                     /gene="sasC"
                     /locus_tag="PZ377_001430"
     CDS             7291..13851
                     /gene="sasC"
                     /locus_tag="PZ377_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDM6358304.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
BASE COUNT         5266 a         2150 c         2592 g         4023 t
ORIGIN      
        1 tgctcaaatt agaaaacaga cccgtaaaaa agaactgaaa gatgagacag tagatgctca
       61 tcttttttta gaactttttt aaggttgaaa atgtatatca cattttatac acatttgatt
      121 tgtaagaaat gttttgattt atacaaatca tatcttgaaa aataaccaat ttagcctcat
      181 tcggtttgat ttaatttgtt aaatttaagg ctaactaatt aatttagttt aatcaatttt
      241 catgagagtt atatgtaata aaaattcaat gcgtatcttt tttgaagaaa tatatgtaga
      301 attgttgcaa tttaatggta atattgatat attttctttg tatataaatt ataaaattaa
      361 tatgtaatag agtgatttgt tttatgtact attatcttat ttctaaatat taactctatt
      421 gattattggt ttttatactt atttaatttt attcaacttt gacaattgaa tagaaagcaa
      481 gtttatttac acttgtagtt ttatgcataa gttagcacat acaagttttg ggattgttgg
      541 gatgtttgtt aatacttgta tagtagctaa atatgtgatt attaattggg agatgtttag
      601 catgaaaaaa gtaaataacg acactgtatt tggaattttg caattagaaa cacttttggg
      661 tgacattaac tcaattttca gcgagattga aagcgaatac aaaatgtcta gagaagaaat
      721 tttaatttta ctaactttat ggcaaaaagg ttctatgacg cttaaagaaa tggacagatt
      781 tgttgaagtt aaaccgtata agcgtacgag aacgtataat aatttagttg aattagaatg
      841 gatttacaaa gagcgtcctg ttgacgatga aagaacagtt attattcatt tcaatgaaaa
      901 gttacaacaa gagaaagtag agttgttgaa tttcatcagt gatgcgattg caagtagagc
      961 aacagcaatg caaaatagtt taaacgcaat tattgctgtg taagtttaat agcataaaaa
     1021 gaggttttca ttaagttgaa aacctctttt tgttgttggc attaattttt caaatgttga
     1081 ctactcaatc ctaaattata aatagtatag cgcagcaaat gcttaagaaa ttttttctat
     1141 ggcacaaatg aatggagcat gattacgttg gtttaaaaat tgatattgca aaacttgcgc
     1201 atgcttttga tccaaagtac tcaagtaatc aagcaatgca tgcttctcaa tttgtccttc
     1261 gctatgacca tgatatataa caagtacaat aataccttca attgacatta atgatagcaa
     1321 tgaattaata gcttggattg tcgtgtcagg ctttgtcacg atagatttat cacctttagg
     1381 caaataacct aggttaaaga tggctgcatc aatatgacct ttatgtgcat catttatatg
     1441 atgttcaata ttttcatgtc catcttttat taaagaaaca tgattgaaat ccttaacttt
     1501 atcacgtgta ttttccaaag ctaaatcttg aatgtcgaaa ccataaacat gtccttctgg
     1561 tacttgttcg gctaaaaata aagtgtcatt gccgttaccg caagttgcgt ctacaacaat
     1621 actttctggt gttatatgtt gtttaataag tgtttttgaa aaagggagta tacgttctaa
     1681 tttcattgct tcaccttaga cttgtaacgc aagccttgat aagaatttct acgtgctaat
     1741 tcagcatcga tgccatttaa tacttcccat ttattaacac tccacattgg acctaccatg
     1801 atatctattg gaccatcacc ggtaattcgg tgaacgatca tttcaggggg aatcacttct
     1861 aattggtcca caactaggtt tgtgtactct tcttgagtca taaaagttaa taaaccttta
     1921 tcgtattgtt ttaccatcgg tgtacctttt aacaaatgaa gtaaatgaat tttaatacct
     1981 tgtacatcca tttgtgcaac ttctttggca gtagccatca tcatgtcata gtcttcgcca
     2041 ggtaagccat taatgatgtg tgtacataca ttgatattat gcttacgtaa ttttgccaca
     2101 ccatcataat aagttttcat atcatgggca cgattgatta aatcagatgt tgactgatgg
     2161 attgtttgta gtcctaattc aacccataag tatgttcgtt gattcaaatc tgctaaatat
     2221 tcgacaacat cgtctggtag acagtcagga cgcgtaccaa tagataatcc cacaacaccc
     2281 ggttctttaa gtacaggttc gaatttttct tttaatactt caaccggtgc atgtgtattt
     2341 gtaaatgcct gaaaataagc aatatatttt ccttcgtgcc atttctcatg catcttttct
     2401 ttaatttctt taaattgtac tgcgattgaa tctgcacgat tacctgcaaa gtctccgcta
     2461 cctgcagcag aacaaaatgt acatccacca tgtgctacag tgccatcgcg gttaggacag
     2521 tcaaacccgc catccaatgc aactttaaat attttttgtc caaatttatt ttttaaatgg
     2581 taattccatg tgtgataacg tttgttttca aaagcgtatt ggaaatgatt gcccatatgt
     2641 cattttcctt tctataaaaa aagagttcta agtacagatt ttaacatatt ttaatgttat
     2701 agtgtttatt atagtttgac aaaaaagaga gaggaactat gaaatatgaa tatacctaaa
     2761 tcagtctggt ggctagtaat tggcatggcg ttaaatatta ctggttccag ttttttgtgg
     2821 cctttaaata caatttatat gaaacaagaa cttggaaaaa gtttaactgt tgctggttta
     2881 gtgctaatga taaattcatt tggcatggtt attggaaact tattaggtgg ttcactattt
     2941 gataaattag gtggatacaa gacgatttta attggaactt tcacttgtct ttgtagtaca
     3001 acgctactta atttctttca cgggtggcct tggtatgctg tatggcttgt aatgttaggg
     3061 tttggtggcg gaatgattat tcctgcgata tacgctatgg ctggagcagt gtggccaaat
     3121 ggcggaagac aaacgtttaa tgcgatatac ttagcgcaaa atattggtgt ggctgtcggt
     3181 gctgcaatgg gcggctttgt cgcagaattt agctttaact atatcttttt agccaatctt
     3241 attatgtatg ttgtgtttgc gcttgtcgcg gtaacgcaat ttaatattga aattaatgcg
     3301 aaagttaaat atccaactca tttagatatt actggtaaaa agaataaagc aagatttatt
     3361 tcattagtac taatttgtgc aatgtttgca atttgttggg ttgcatatat tcaatgggag
     3421 tctacaatcg cttcatttac acaatctatt aatatttcaa tggcacaata tagtgtttta
     3481 tggacaatta acggaataat gattttagta gcacaaccat taattaaacc gattctctat
     3541 ctgttaaaag gaaacttaaa gaagcaaatg tttgtcggca tcatcatttt tatgttgtcg
     3601 ttctttgtca cgagttttgc cgaaaacttt acaatatttg ttgtcggtat gattatttta
     3661 acttttggag aaatgtttgt atggccagca gttccaacta tagccaatca gttagcgcca
     3721 gatggtaagc aaggacagta ccaaggtttt gtgaattcag ctgctacagt aggaaaagca
     3781 tttggtccat ttcttggtgg tgtattagtt gatgcgttta atatgcgcat gatgtttatc
     3841 ggtatgatgc tactacttgt atttgcatta atattattaa tggttttcaa ggagaataat
     3901 acgcaaccta aaaaaataga tgcataatga gtaaatagaa ttaacgttat agacttgaaa
     3961 taaatgtcgt tataacataa tattaatttg tataatttaa tttcgtttgg agcttttcta
     4021 cagaaagcta gtgatgctga gagctagtgt taaggactaa atgtaaatcg tattaatttt
     4081 aaattgaatg aatgacatct cttactatta aaatgagtgc acaatttttg tgaaataggg
     4141 tggtaacgcg gcaaatgtcg tccctatgta aatagaatag ttagaggtgt cttttttatt
     4201 gaataggagg aaatgtgttg aattataacc acaatcaaat tgaaaagaaa tggcaagact
     4261 attgggacga aaataaaaca tttaaaacaa atgataactt aggtcaaaag aaattttatg
     4321 ctttagacat gtttccatat ccatcaggtg ctggtttaca tgttggacat cctgagggct
     4381 atacagcaac agatatcatt tcaagatata aaagaatgca aggatataat gtattacatc
     4441 cgatggggtg ggatgcattc ggattaccag cagagcaata tgctttagac actggcaacg
     4501 acccacgtga atttacaaag aaaaatatcc aaacttttaa acgacaaatt aaagaattag
     4561 ggttcagtta tgattgggat cgtgaagtta atacaacaga tccagaatac tataaatgga
     4621 cacagtggat tttcatacag ttatataaca aaggtttagc atacgttgat gaagttgcag
     4681 ttaactggtg tccagcatta ggcactgttt tatctaacga agaagtgatt gatggtgtct
     4741 ctgaacgtgg tggacatcca gtttatcgta agccgatgaa acaatgggta cttaaaatca
     4801 cagaatatgc agatcaatta ttagcagatt tagatgattt agattggcct gagtctttaa
     4861 aagatatgca gcgcaattgg attggacgtt ctgaaggggc caaagtttca tttgatgtag
     4921 ataatacgga aggaaaagta gaagtattta cgactagacc agatacaatc tatggtgcat
     4981 cattcttagt cttaagtcct gaacatgcat tagttaattc aattacaaca gatgaatata
     5041 aagaaaaagt aaaagcttat caaacagaag cttctaaaaa gtcagattta gaacgtacag
     5101 atttagcaaa agataaatca ggtgtattta ctggtgcata tgcaactaat cctttatctg
     5161 gtgaaaaagt acaaatttgg attgctgatt atgtattatc aacatatggt actggagcaa
     5221 ttatggcagt accagcgcat gatgacagag attatgaatt tgctaaaaag tttgatttgc
     5281 caatcattga agtcatcgaa ggtggaaatg ttgaagaagc agcatacact ggtgaaggta
     5341 aacatattaa ttctggtgaa cttgatggtt tagaaaatga agcggcaatt actaaagcta
     5401 ttcaattatt agagcaaaaa ggtgctggcg aaaagaaagt taattacaaa ttaagagatt
     5461 ggttattcag tcgtcagcgt tattggggcg aaccaattcc tgtcattcat tgggaagatg
     5521 gaacaatgac aactgttcct gaagaagagc taccattgtt gttacctgaa acagatgaaa
     5581 tcaagccatc agggactggt gagtctccac tagctaatat tgattcattt gtaaatgttg
     5641 tagatgaaaa aacaggtatg aaaggacgtc gtgaaacaaa tacaatgcca caatgggcag
     5701 gtagttgttg gtattattta cgttacatcg atcctaaaaa tgaaaatatg ttagcagatc
     5761 ctgaaaaatt aaaacattgg ttacctgttg atttatatat cggtggagta gaacatgcgg
     5821 ttcttcactt attatatgca agattttggc ataaagtcct ttatgatttg gctatcgtac
     5881 ctactaaaga acctttccaa aaattattta accaaggtat gattttagga gaaggtaatg
     5941 agaagatgag taaatctaaa ggaaatgtaa tcaatcctga tgatatagta cagtctcatg
     6001 gtgcagatac tttgcgtctt tacgaaatgt ttatgggacc tttagatgct gcaattgcat
     6061 ggagtgaaaa aggattagat gggtctcgtc gattcttaga tcgcgtatgg cgtttaatgg
     6121 taaatgaaga tgggacattg agttcaaaaa ttgtaactac aaataataaa tctttagata
     6181 aagtttataa ccaaactgtt aaaaaggtaa cagaagactt tgaaacatta ggatttaata
     6241 ctgctattag tcaattaatg gtatttatta atgagtgtta taaagttgat gaagtttata
     6301 aaccttacat tgaaggcttc gttaaaatgt tagcacctat tgcaccacat atcggtgaag
     6361 aattatggtc aaaattagga catgaagagt ctattacgta ccaaccttgg ccaacttatg
     6421 acgaagcact acttgtagat gatgaagtag aaatcgttgt tcaagtgaat ggtaaattga
     6481 gagctaaaat taaaattgct aaagatacat caaaagaaga aatgcaagaa attgccttat
     6541 ctaatgacaa tgttaaagcg agtattgaag gtaaagacat catgaaagtc atcgctgttc
     6601 ctcaaaaatt agtcaatatt gtagctaaat aatgttttaa ggaggacttt gaaatgaagt
     6661 caattactac agatgaatta aaaaataaac ttttagaatc taaaccagtt caaattgttg
     6721 atgttcgtac tgatgaagaa acagcaatgg gatatattcc taatgcaaag ttaattccaa
     6781 tggataccat tccggataat ttaaattcat ttaataaaaa tgaaatatat tatattgtat
     6841 gtgctggtgg agttcgaagc gctaaagttg tagaatattt agaggcaaat ggcattgatg
     6901 ccgtaaatgt cgaaggcggc atgcacgcat ggggcgatga aggtttggaa ataaaaagta
     6961 tttaaagtag tgacataatt taaaataata ttacatttgt aatgacacca agtaacgttt
     7021 cggttgcttg gtgttttttg gtatgaatta ctttctgtta caaaacaatc taaagcgttc
     7081 ttgttatgtt ttattaagat tttaattaca aaacggaaac taaattgtaa taaaataaaa
     7141 ctttatttta taaaatgatg atgataaaat tgagtgaact taaaatattg tacaaaataa
     7201 tatagctata aatataatat gagggagcgt atatttttag cataattctt aacaacacag
     7261 cagagaacag acaaccagga ggaaaatgaa atgaatttgt taaagaaaaa taaatatagt
     7321 attaggaagt ataaagtagg catattctct actttaatcg gaacagtttt attactttca
     7381 aacccaaatg gtgcacaagc cttaactacg gataataatg tacaaagcga tactaatcaa
     7441 gcaacacctg taaattcaca agataaagat gttgctaata atagaggttt agcaaatagt
     7501 gcgcagaata cacctaatca atctgcaaca accaatcaag caacgaatca agcattggtt
     7561 aatcataata atggtagtat agtaaatcaa gctacgccaa catcagtgca atcaagtacg
     7621 ccttcagcac aaaacaataa tcatacagat ggcaatacaa cagcaactga gacagtgtca
     7681 aacgctaata ataatgatgt agtgtcgaat aataccgcat taaatgtacc aactaaaaca
     7741 aatgaaaatg gttcaggagg acatctaact ttaaaggaaa ttcaagaaga tgttcgtcat
     7801 tcttcaaata aaccagagct agttgcaatt gctgaaccag catctaatag accgaaaaag
     7861 agaagtagac gtgcggcacc ggcagatcct aatgcaactc cagcagatcc agcggctgca
     7921 gcggtaggaa acggtggtgc accagttgca attacagcgc catatacgcc aacaactgat
     7981 cctaatgcca ataatgcagg acaaaatgca cctaacgaag tgctgtcatt tgatgacaat
     8041 ggtattagac caagtaccaa ccgttctgtg ccaacagtaa acgttgttaa taacttgccg
     8101 ggcttcacac taatcaatgg tggcaaagta ggggtgttta gtcatgcaat ggtaagaacg
     8161 agcatgtttg attcaggaga taataagaac tatcaagcac aaggaaatgt aattgcatta
     8221 ggtcgtatac atggaactga tacgaatgac catggcgatt ttaatggtat cgagaaagca
     8281 ttaacagtaa atccgaattc tgaattaatc tttgaattta atacaatgac tactaaaaac
     8341 ggtcaaggcg caacaaatgt tattatcaaa aatgctgata ctaatgatac gattgctgaa
     8401 aagactgttg aaggcggtcc aactttgcgt ttatttaaag tacctgataa tgtgagaaat
     8461 ctcaaaattc aatttgtacc taaaaatgac gcaataacag atgcgcgtgg catttatcaa
     8521 ctaaaagatg gttacaaata ctatagcttt gttgactcta tcggacttca ttctgggtca
     8581 catgtttttg ttgaaagacg aacaatggat ccaacagcaa caaataataa agagtttact
     8641 gtaacaacat cattaaagaa taatggtaat tctggtgctt ctctagatac aaatgacttt
     8701 gtatatcaag ttcaattacc tgaaggtgtt gaatatgtga acaattcatt gactaaagat
     8761 tttccaagta acaattcagg cgttgatgtt aatgatatga atgttacata tgatgcagca
     8821 aatcgtgtga taacaattaa aagtactgga ggaggtacag caaactctcc ggcacgactt
     8881 atgcctgata aaatactcga tttaagatat aaattacgtg taaataatgt gccgacacca
     8941 agaacagtaa catttaacga gacattaacg tataaaacat atacacaaga tttcattaat
     9001 tcagctgcag aaagtcatac tgtaagtaca aatccatata ctatcgatat catcatgaat
     9061 aaagatgcat tacaagccga agttgacaga cgtattcaac aagctgatta tacatttgcg
     9121 tcattagata tctttaatgg tctgaaacga cgcgcacaaa cgattttaga tgaaaatcgt
     9181 aacaatgtac cattaaataa aagagtttct caagcatata ttgattcatt aactaatcaa
     9241 atgcaacata cgttaattcg aagtgttgat gctgaaaatg cagttaataa aaaagttgac
     9301 caaatggaag atttagttaa tcaaaatgat gaattgacag atgaagaaaa acaagcagca
     9361 atacaagtta tcgaggaaca taaaaatgaa ataattggta atattggtga ccaaacgact
     9421 gatgatggcg ttactagaat caaagatcaa ggtatacaga ccttaagtgg ggatactgca
     9481 acaccggttg ttaaaccaaa tgctaaaaaa gcaatacgtg ataaagcaac gaaacaaagg
     9541 gaaattatca atgcaacacc agatgttact gaagacgaga ttcaagatgc actaaatcaa
     9601 ttagctacgg atgaaacaga tgctattgat aatgttacga atgctactac aaatgctgac
     9661 gttgaaacag ctaaaaataa tggcatcaat actattggag cagttgttcc tcaagtaact
     9721 cataaaaaag ctgcaagaga tgcaattaac caagcaacag caacgaaaag acaacaaata
     9781 aatagtaata gagaagcaac tcaggaagag aaaaatgcag cattgaacga attaactcaa
     9841 gcaaccaacc atgctttaga acaaatcaat caagcaacaa caaatgctga tgttgataac
     9901 gccaaaggag atggtctaaa tgccattaat ccaattgctc ctgtaactgt tgttaagcaa
     9961 gctgcaaggg atgccgtatc acatgatgca caacaacata tcgcagagat caatgctaat
    10021 cctgatgcga ctcaagaaga aagacaagca gcaattgaca aagtgaatgc tgctgtaact
    10081 gcagcaaaca caaacatttt aaacgctaat accaatgctg atgttgaaca agtaaagaca
    10141 aatgcgattc aaggaataca agcaattaca ccagctacaa aagtaaaaac agatgcaaaa
    10201 aatgccatcg ataaaagtgc ggaaacgcaa cataatacga tatttaataa taatgatgcg
    10261 acgctcgaag aacaacaagc agcacaacaa ttacttgatc aagctgtagc cacagcgaag
    10321 caaaatatta atgcagcaga tacgaatcaa gaagttgcac aagcaaaaga tcagggcaca
    10381 caaaatatag tagtgattca accggcaaca caagttaaaa cggatactcg caatgttgta
    10441 aatgataaag cgcgagaggc gataacaaat atcaatgcta caactggcgc gactcgagaa
    10501 gagaaacaag aagcgataaa tcgtgtcaat acacttaaaa atagagcatt aactgatatt
    10561 ggtgtgacgt ctactactgc gatggtcaat agtattagag acgatgcagt caatcaaatc
    10621 ggcgcagttc aaccgcatgt aacgaagaaa caaactgcta caggtgtatt aaatgattta
    10681 gcaactgcta aaaagcaaga aattaatcaa aacacaaatg caacaactga agaaaagcaa
    10741 gtggctttaa atcaagtgga tcaagagtta gcaacggcaa ttaataatat aaatcaagct
    10801 gatacaaatg cggaagtaga tcaagcgcaa caattaggta caaaagcaat taatgcgatt
    10861 cagccaaata ttgttaaaaa acctgcagca ttagcacaaa tcaatcagca ttataatgct
    10921 aaattagctg aaatcaatgc tacaccagat gcaacgaatg atgagaaaaa tgctgcgatc
    10981 aatactttaa atcaagacag acaacaagct attgaaagta ttaaacaagc taacacaaat
    11041 gcagaagtag accaagctgc gacagtagca gagaataata tcgatgctgt tcaagttgat
    11101 gtagtaaaaa aacaagcagc gcgagataaa atcactgctg aagtggcgaa gcgtattgaa
    11161 gcggttaaac aaacacctaa tgcaactgac gaagaaaagc aggctgctgt taatcaaatc
    11221 aatcaactta aagatcaagc aattaatcaa attaatcaaa accaaacaaa tgatcaggta
    11281 gacacaacta caaatcaagc ggtaaatgct atagataatg ttgaagctga agtagtaatt
    11341 aaaccaaagg caattgcaga tattgaaaaa gctgttaaag aaaagcaaca gcaaattgat
    11401 aatagtcttg attcaacaga taatgagaaa gaagttgctt cacaagcatt agctaaagaa
    11461 aaagaaaaag cacttgcagc tattgaccaa gctcaaacga atagtcaggt gaatcaagca
    11521 gcaacaaatg gtgtatcagc gattaaaatt attcaacctg aaacaaaagt taaaccagct
    11581 gcacgtgaaa aaatcaatca aaaagcgaat gaattacgtg ctaagattaa tcaggataaa
    11641 gaagcaacag cagaagaaag acaagtagca ctagataaaa tcaatgaatt tgtaaatcaa
    11701 gccatgacag atattacgaa taatagaaca aatcaacaag ttgatgatac aacaagtcaa
    11761 gcgcttgata gcattgcttt agtgacgcct gaccatattg ttagagcagc tgctagagat
    11821 gcagttaagc aacaatatga agctaaaaag cgcgaaattg agcaagcgga acatgcgact
    11881 gatgaagaaa aacaagttgc tttaaatcaa ttagcgaata atgaaaaacg tgcattacaa
    11941 aacatcgatc aagcaatagc gaataatgat gtgaaacgtg ttgaaacaaa tggcattgct
    12001 acactaaaag gtgtacaacc tcatattgta attaagcctg aagcacaaca agcaataaaa
    12061 gcaagtgcag aaaatcaagt agaatcaata aaagatacac cacatgcaac agttgatgaa
    12121 ttagatgaag cgaatcaatt aattagcgac acactcaaac aagcgcaaca agaaatagaa
    12181 aatacaaatc aagatgctgc tgttactgat gttagaaatc aaacaatcaa ggcaatagag
    12241 caaataaaac ctaaagtaag acgtaaacga gctgcgcttg atagcattga agaaaataat
    12301 aaaaatcaac tcgatgcaat ccgaaatacg ttggatacta ctcaagatga aagagatgtt
    12361 gctattgata ctttaaataa aattgtaaat acaattaaaa atgacattgc acaaaacaaa
    12421 acgaatgcag aagtggatcg aactgagact gatggcaacg acaacatcaa agtgatttta
    12481 cctaaagttc aagttaaacc agcagcgcgt caatctgttg gtgtaaaagc cgaagctcaa
    12541 aatgcactaa tcgatcaaag cgatttatca actgaagaag aaagactagc tgctaaacat
    12601 ttagtagaac aagcacttaa tcaggctatt gatcagatca atcatgcaga taagactgcc
    12661 caagttaatc aagatagtat aaatgctcaa aatattattt caaaaattaa accagcgaca
    12721 acagttaaag caacagcatt acaacaaatt caaaatatcg ctacaaataa aattaattta
    12781 attaaagcaa ataacgaagc gacagatgaa gaacaaaata ttgcaatagc acaagttgaa
    12841 aaagagttaa ttaaagctaa acaacaaatt gctagtgcag tgactaatgc agatgtggca
    12901 tatttattgc atgatgagaa aaacgaaatt cgtgaaatcg aacctgttat taacagaaag
    12961 gcgtctgctc gagaacaatt gacaacatta ttcaacgata aaaaacaagc aattgaagcg
    13021 aatattcaag caacggtaga agaaagaaat agtatattag cacagttaca aaatatttat
    13081 gacactgcta ttggacaaat tgatcaagat cgtagcaatg cacaagttga taaaacagca
    13141 tcattaaatc tacaaacaat acatgattta gatgtacatc ctattaaaaa gccagatgct
    13201 gaaaaaacga ttaatgatga tcttgcacgc gtcactgctt tagtgcaaaa ttatcgaaaa
    13261 gtaagtaatc gtaataaggc tgatgcatta aaagctataa ctgctttaaa attacaaatg
    13321 gatgaagaat taaaaacagc acgcactaat gctgatgttg atgcagtttt aaaacgattt
    13381 aatgttgcat taagcgatat agaagcagta attactgaaa aagaaaatag cttactgcga
    13441 attgataaca ttgctcaaca aacatatgcg aaattcaaag cgatcgcaac accagaacaa
    13501 ttagctaaag taaaagtatt aattgatcaa tatgttgcag atggcaatag aatgattgat
    13561 gaagatgcga cattaaatga catcaaacaa cacacgcaat tcattgttga tgaaatttta
    13621 gcaattaaat taccagctga agcgacgaaa gtatcaccaa aagaaattca gccagctcca
    13681 aaagtttgta cgcctattaa aaaagaagag acacatgaat cgcgcaaagt tgaaaaagaa
    13741 cttccaaata caggttctga aggaatggat ttaccattga aagaatttgc actgattaca
    13801 ggtgcggctt tgttagctag aagacgtact aaaaacgaaa aagaatcata attaacgaaa
    13861 tgaccttaat ataacgacac tttttagtga ctcagttata ttaaggtcat ttttatttga
    13921 atgaaaaagt tgatctacta aagagaatcg aaccaaattt tatactatta ttccattgat
    13981 gcatgtgagt aatgtccggc atataatcca gcgacatgtc ctgttacgag t
//
LOCUS       DANRDI010000063        13782 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.063, whole genome shotgun sequence.
ACCESSION   DANRDI010000063 DANRDI010000000
VERSION     DANRDI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.063"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(193..1122)
                     /locus_tag="PZ377_001431"
     CDS             complement(193..1122)
                     /locus_tag="PZ377_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDM6358305.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(1119..1955)
                     /locus_tag="PZ377_001432"
     CDS             complement(1119..1955)
                     /locus_tag="PZ377_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDM6358306.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(1949..2692)
                     /locus_tag="PZ377_001433"
     CDS             complement(1949..2692)
                     /locus_tag="PZ377_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDM6358307.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            2814..3458
                     /locus_tag="PZ377_001434"
     CDS             2814..3458
                     /locus_tag="PZ377_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDM6358308.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(3538..4290)
                     /locus_tag="PZ377_001435"
     CDS             complement(3538..4290)
                     /locus_tag="PZ377_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDM6358309.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            4526..5290
                     /locus_tag="PZ377_001436"
     CDS             4526..5290
                     /locus_tag="PZ377_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDM6358310.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(5351..6145)
                     /gene="tagH"
                     /locus_tag="PZ377_001437"
     CDS             complement(5351..6145)
                     /gene="tagH"
                     /locus_tag="PZ377_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDM6358311.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            6492..7304
                     /gene="tagG"
                     /locus_tag="PZ377_001438"
     CDS             6492..7304
                     /gene="tagG"
                     /locus_tag="PZ377_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDM6358312.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            7403..8506
                     /locus_tag="PZ377_001439"
     CDS             7403..8506
                     /locus_tag="PZ377_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDM6358313.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            8503..9564
                     /locus_tag="PZ377_001440"
     CDS             8503..9564
                     /locus_tag="PZ377_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDM6358314.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            9627..10025
                     /gene="tagD"
                     /locus_tag="PZ377_001441"
     CDS             9627..10025
                     /gene="tagD"
                     /locus_tag="PZ377_001441"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDM6358315.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(10142..11437)
                     /gene="pbp4"
                     /locus_tag="PZ377_001442"
     CDS             complement(10142..11437)
                     /gene="pbp4"
                     /locus_tag="PZ377_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDM6358316.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            11858..13585
                     /locus_tag="PZ377_001443"
     CDS             11858..13585
                     /locus_tag="PZ377_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358317.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
BASE COUNT         4748 a         2060 c         2327 g         4647 t
ORIGIN      
        1 ttctaatacc accatatcgt gcgatcggga acggtatata tattaatagg agggtaatat
       61 atatttaacg cacgatatgg gactattagc cttcgacttt gttatgttga tgtgtggcct
      121 aaaatattgg agataccaat attttaggtt gcatcaacat caattcatct tacttcatta
      181 aaacacagcg tgttatttca tgcttccgtg tacagtttca atatttgatt tcatcatttt
      241 gtagtaagag tcacctttag tgccttcttt accgattgaa tctgtgtaca cttcaccaaa
      301 gatatctttc ttcgtttctt cagataaact ttccattgct ttcttatcaa cacttgtttc
      361 tactaataag tgttttaatt tgtgcttttt aacaaactca atagcttgtc tcatttgttc
      421 aggtgtacct tgtttttcag tgttaatttc ccaaatataa cctggtgtaa taccgtattg
      481 ttttgagaag tacttgaagg caccttcact tgtaatcatg gcacgttgtt cttttggaat
      541 gtcattaaat ttgtctttac tgtcattatt taatttttcc aattgagcaa tgtatttgtt
      601 accttgcttt tcataatctg ctttatgttt tttgtcgtta tcgataaatg tttgttgaat
      661 tgtttttacg tatttaatac cgttatctaa acttaaccat gcgtgtggat cttgtttatc
      721 tttgttgcct tcttcaccgt ttaaatagat aggtttaaca tcttttgata ctgcgataac
      781 ttttttatct tttaatgatt taccagcctg ttctaaggct ttttcaaacc aaccgttacc
      841 agtctctaaa tttaatccgt tgtataaaat aacgtcagcg tcagttaact ttttaatatc
      901 tttaggttta acttcatatt catgaggatc ttgaccaaca ggtacaatac tatgaatatc
      961 gacgttgtct ccaccaacat ttttagccat atcatataaa attgaattcg tcgttactac
     1021 ttttaatttg ccatttgact tatcactgct ttgtttacca ccagtaccac atgcagcaac
     1081 tagaagtaat aaggctaata ataaaggtac taattttttc atgttaaact tcctcgtttc
     1141 tttctattcg taaattttgt gaaaaataat gtgatgatat aaattacaaa cgtacaaagt
     1201 acgattgtcg caccactagg aatgttgtaa atatagctgt aataaagtcc gacaattgaa
     1261 cttatgacac ttattaaact tgctataatc atcattgagt atagtttttt actaattaaa
     1321 aatgctgtag atgcaggtgt aattaataat gcaactacaa gaataatacc taccgtttga
     1381 atacttgcta ctgttactaa tgagagtaac aacatcacaa agtaatgtaa taacgtcgta
     1441 tttagaccac tcattctact aaacgttgga tcgaatgtag aaatcattaa tggacgatag
     1501 aaaataatga ttagaataag gacgattgaa ccaatcacaa tagttgttaa aaatgcacta
     1561 tttgtgattg ccagtaaatt accaaacaga atatggtaca aatctgtcgt agtgtttatt
     1621 aagctaataa taataatccc cgaagctaag aaagcggtaa aactaattcc aatagcggcg
     1681 tcaggtttcg ttttactact agatgtgata taaccgataa aaatacttgc gatcatacca
     1741 gttataagtg cgcctacaaa cattggaata ccaaataaga atgatagggc aacaccaggt
     1801 aatactgcgt gactcattgc atctcccatt aatgaaagac cacgtaatac aattaaacta
     1861 ccaactgtac cacaaactat ccctacaata attgaagtta tcaatgctcg attcaagaat
     1921 tgatatgtaa ataaatgttc gacaaactct aacatgttat attgctcctt tgactagggt
     1981 cactacagtc agtgctactc ataaatgttt cgtttaagcg agtgacactc atagcctctt
     2041 cactatcacc aaagtatcgt aatgtttgat ttaatagaat aatgcgatca aagtattgct
     2101 ttgcttttga tagatcatgg tggatgataa gaataagttt tccttgttgt tttaagttct
     2161 cgatttttgt catgattaat ttttcgctac taaaatcaat tccgacaaac ggctcatcta
     2221 gaaaataaac ttcactttcg gacatcaatg ctcttgctac tagcacacgt tgtaattgtc
     2281 caccacttaa ttctgaaatt tgtcgatgac gtaaagattc taattctaaa tcgcttaata
     2341 actgtttgag tttatccctt gctgatttat taggtcgtct aaaccatcca atttctttgt
     2401 agcaacctga taaaatcact tgttccacac ttataggaaa atctaaatca atatgtgctt
     2461 tttgtggaat atatgtaata tgttgcagtt gttgttgtat aggtttgtta tataacaatt
     2521 tagtaccggt agcattaaat tcaccaatta aagacttgat aagggaagat ttaccagcac
     2581 cattcgggcc catgatacca attatttcgc cgcgtactgg tatcgataag gaaatgtttt
     2641 taagtacatg cttattacct aaaaacagat ttaaatcttt tgtttctaac aaacgtttat
     2701 acctcctaat taaaagttta ggctaaccta attaattgta taataaactg agaatattta
     2761 tcatgtcaag taaattcgtg atataatata gacaatgtat gtgaggtgaa agtatgttaa
     2821 ctgaagaaaa agaggactat ttaaaggcaa tccttacgaa taatggcgat aaaaactttg
     2881 tgacaaataa aatcttatct caatttttaa atattaagcc tccatctgta agtgaaatgg
     2941 taggacgtct tgaaaaagca ggctatgttg aaacaaaacc atacaaaggt gttagattaa
     3001 cagaggatgg tttaacgcat acgcttgata tcattaagag acatcgacta ttagaattat
     3061 ttttaataga aatattgaaa tataattggg aagaagtaca tcaagaagca gaaattttag
     3121 aacatcgaat ttcagattta tttgttgaaa ggctggatag cctgttaaat ttcccagaaa
     3181 cttgcccgca cggcggtgtg attcctagaa ataatgaata taaagagaaa tatataacaa
     3241 cgattttgaa ttatgaacct ggtgatatcg ttacaatcaa acgtgtgaga gataagaccg
     3301 atttgctaat atatttgtct agtaaagata tttctattgg taatgaagtg gaaattgtat
     3361 cgaaagatga aatgaataaa gtaattatca ttaaacgtaa tgataatgta attattgtca
     3421 gttacgaaaa tgcaatgaac atgtttgctg aaaaataaaa taaagaagcc ataaagatat
     3481 ccatgattga actgataaag acatatggat aattgcttta ggcttctttt ttattagtta
     3541 atttatcaag tgagtatatt tgagtaaaat attcactgca taaagattga agataatcca
     3601 gattgtacta taaatgaaga taggtacatg actgagttct ttaagtgcac taccatccca
     3661 ctgtggactc ggacgctgga aagtcaattt agcaatcgtc caactagatt gtagaacttc
     3721 gcctaataat acacctaaaa tatattgata actcattgtg acaagtagtt gaatttctac
     3781 tatattttca tcttttaata taaaatacaa catgatagaa attaaagtta taacaacaat
     3841 gggtgagcct tttctagatg ttaaaattaa aaaataaata aatatcaata aataggtaaa
     3901 tataaagaaa ctaggtatct gataatggct cgacgctaaa cctatcaata acataatagg
     3961 tggcataaaa taaccaccaa tcgttgtaag ccattggcct gctagatgtc tagattgtgt
     4021 aattgcgaat ccttgttgta atgtctgttg tcgctctcgt ggacttgtta caatgactaa
     4081 atcttttgca cggccaccag cgagtttatt aaacagtaca tgaccaaatt catgtgttaa
     4141 aacagggata tagtttaaaa tgacatctaa atagttcaaa acaggcttat gtctatattg
     4201 atgaatagca atataacaag ctgcaacaat aacgataatg tatatattaa gttgaattgt
     4261 cgtattaaaa aagtttgata aataattcat tgttaacctc atataagata ttaatttaaa
     4321 gtttgcttat cacttattat aaatgatatt ggcatcaata gcgttagact ttagacttac
     4381 cttagttaaa ctaattttaa tttttgaaaa ggtgaatatg tgttaaaata aagcaaaatc
     4441 atttcgatat aaataggatg aatataaata ctgttaatat tgattacact aacataataa
     4501 tgaaataaga taggagattc ctgttatgac tgttgaagaa agatccaata cagccaaagt
     4561 tgacatttta ggggtcgatt ttgataatac aacaatgttg caaatggttg aaaatattaa
     4621 aacctttttt gcaaatcaat caacgaataa tctttttata gtaacagcca accctgaaat
     4681 agtgaattac gcgacgacac atcaagcgta tttagagtta ataaatcaag cgagctatat
     4741 tgttgctgat gggacaggag tagtcaaagc ttcgcatcgt ttaaagcaac ctctagcgca
     4801 tcgtatacct ggtattgagt tgatggatga atgtttgaaa attgctcatg taaatcatca
     4861 aaaagtattt ttgctagggg caactaatga agttgtagaa gcggcacaat atgcattgca
     4921 acaaagatat ccaaacatat cgtttgcaca tcatcacggt tatattgatt tagaagatga
     4981 gacagtagtg aaacgaatta aactgtttaa acctgattac atatttgtag gtatgggatt
     5041 ccctaaacaa gaagaatgga ttatgacaca tgaaaaccaa tttgaatcta cagtgatgat
     5101 gggcgtaggt ggttctcttg aagtatttgc tggggctaaa aagagagcgc cttatatctt
     5161 tagaaaatta aacattgaat ggatatatag agcattaata gattggaaac gtattggtag
     5221 attaaagagt attccaatat ttatgtataa aatagccaaa gcaaaaagaa aaataaaaaa
     5281 ggcgaaataa tcatgatgac aaaaataaaa ccgaggaaat ccttaaatgg agattctcgg
     5341 ttttttcggt ttatttaata acgaagcggg actcatcgag tttgtttcta aattcttttt
     5401 gttcggcttt ggatttcttt ttaaaatcgt taaggaaagc ttcatattta ggtaatacat
     5461 catcaagttc accgtaatct tttaactttc cgccttcaat ccaagcaatc ttagtacaaa
     5521 attgtctcac ttgtcctaag ttatgactaa cgaaaaagat ggttttgttt tgctctttaa
     5581 actcgtaaat tttatctaaa catttttgtg caaaagtttg gtcacctaca gataaagctt
     5641 cgtcaatgac taagatatct ggattaactg tgatattaat tgaaaaacca agttttgcac
     5701 gcataccact tgaatacttt ttaactggtt gataaataaa ctcaccaagt tcactaaatt
     5761 caataatctt aggtgtcatc gctttaattt cttttcgctt aaagcccata cataacattt
     5821 taaattcgat attttcaatc cctgtaagtt gtccactcaa gccagcacta attgcgataa
     5881 cgctgacttc accattacga tccactttgc caacagtagg cgacaaagaa ccgccaatga
     5941 tattgctcaa cgttgatttg ccggaaccat tgatgccaac aagccctatg acgtcacctt
     6001 catatgcttt taaactaatg tcatctaaag cgaaaaatgt tttgttttta tgtttgggaa
     6061 tgagcgcatc tttcatacgt tctttatttg tacgataaat acgatattct tttgttacat
     6121 ttttaatgtt taccgaaacg ttcatttgta gaccttcctt attcacattt atctagatta
     6181 taatatacta ctcaacagtt gttaaatttt aaaacctgtt gtaaagtgta tagaagattt
     6241 tgttattatc agagtgggtg ttttgacaca aaatgttaat catcaatgat aacaatgata
     6301 tttaaaaact aaacttattt caacttacat gattgtatac tataatgtat ttgtaataaa
     6361 ctaatatttt aaagaactag acaataattt tgatagcatc catgtatagt gatagtattt
     6421 acaacaatta ttataatact atttagttaa gtagagaaat agttaaacat ttgaaagtgt
     6481 ggtttaatgg aatgtcagca ataggaacag tttttaaaga acatgtaaag aacttttatt
     6541 taattcaaag actggctcag tttcaagtta aaattatcaa tcatagtaac tatttaggtg
     6601 tggcttggga attaattaac cctgttatgc aaattatggt ttactggatg gtttttggat
     6661 taggaataag aagtaatgca ccaattcatg gtgtaccttt tgtttattgg ttattggttg
     6721 gtatcagtat gtggttcttc atcaaccaag gtattttaga aggtactaaa gcaattacac
     6781 aaaagtttaa tcaagtatcg aaaatgaact tcccgttatc gataataccg acatatattg
     6841 tgacaagtag attttatgga catttaggct tacttttact tgtgataatt gcatgtatgt
     6901 ttactggtat ttatccatca atacatatca ttcaattatt gatatatgta ccgttttgtt
     6961 ttttcttaac tgcctcggtg acgttattaa catcaacact cggtgtgtta gttagagata
     7021 cacaaatgtt aatgcaagca atattaagaa tattatttta cttttcacca attttgtggc
     7081 taccaaagaa ccatggtatc agtggtttaa ttcatgaaat gatgaaatat aatccagttt
     7141 actttattgc tgaatcatac cgtgcagcaa ttttatatca cgaatggtat ttcatggatc
     7201 attggaaatt aatgttatac aatttcggta ttgttgccat tttctttgca attggtgcgt
     7261 acttacacat gaaatataga gatcaatttg cagacttctt gtaatatatt tatatgacga
     7321 aaccccgcta accattaata aatggaagtg gggttcattt ttgtttataa tttaagtaaa
     7381 taacatatta agttggtgta ttatgaacgt tttaataaag aaattttatc atttggtagt
     7441 tcgaatactt tctaaaatga ttacgcctca agtgattgat aaaccgcata tcgtatttat
     7501 gatgactttt ccagaagata ttaagcctat catcaaagca ttaaataatt cgtcgtatca
     7561 gaaaactgtt ttaacaacac caaaacaagc gccttattta tctgaactta gcgacgatgt
     7621 tgatgtgata gaaatgacta atcgaacatt ggtaaaacaa attaaggctt tgaaaagcgc
     7681 gcagatgatt attatcgata attattacct attactaggt ggatataata agacttctaa
     7741 tcaacacatt gttcaaacgt ggcatgcaag tggtgcatta aaaaactttg gcttaacaga
     7801 tcatcaagtc gatgtgtctg acaaggcaat ggttcagcag taccgtaaag tttatcaagc
     7861 gacggatttt tacttagtgg gttgtgaaca aatgtcacaa tgttttaaac agtctttagg
     7921 tgcaacagaa gagcaaatgc tgtattttgg gcttccgaga attaataaat attacacagc
     7981 tgatagagca acggttaagg cagagttaaa ggataaatat ggaattacaa ataagttggt
     8041 attatatgta ccaacatata gagaagataa agcagataat agggctattg ataaagctta
     8101 ttttgaaaaa tgtttaccag gatatacact gattaataaa ttacatccat caattgaaga
     8161 ttcagacatt gatgacgtat cttcaatcga cacgtctaca ttaatgctaa tgtcagatat
     8221 aattattagc gactatagtt cgctgccaat agaagctagc ttgttagata ttccaactat
     8281 attttatgtg tatgatgaag gaacatatga tcaggtgaga ggcctgaatc aattttacaa
     8341 agcaataccg gatagctaca aagtgtatac tgaagaagat ttaataatga cgatacaaga
     8401 aaaagaacat ctattaagtc cgttatttaa agattggcat aagtataata ctgataaaag
     8461 tttacatcag ctcacagaat atatagataa gatggtgaca aaatgaggtt tacgataatc
     8521 atacctacat gtaataatga ggcaacaatt cgacaattgt taatatctat tgagagtaaa
     8581 gaacactata gaatcctttg tattgatggt ggttctactg atcaaacaat tcctatgatt
     8641 gaacggttac aaagagaact caagcatatt tcattaatac aattacaaaa tgcttcgata
     8701 gctacgtgta ttaataaagg tttgatggat atcaaaatga cagatccaca tgatagtgac
     8761 gcatttatgg tcataaaacc aacatcaatc gtattgccag gtaaattaga taggttaact
     8821 gctgctttca aaaataatga taatattgat atggtaatag ggcagcgagc ttacaattac
     8881 catggtgaat ggaaattgaa aagtgctgat gagtttatta aagacaatcg aatcgttaca
     8941 ttaacggaac aaccagattt gttatcaatg atgtcttttg acggaaagtt attcagtgct
     9001 aaatttgctg aattacagtg tgacgaaact ttagctaaca catacaatca cgcaatactt
     9061 gtcaaggcga tgcaaaaagc tacggatata catttagttt cacagatgat tgtcggagat
     9121 aacgatatag atacacatgc tacaagtaac gaagaagatt ttaatagata tatcacagaa
     9181 attatgaaaa taagacaacg agtcatggaa atgttactat tacctgaaca aaggctatta
     9241 tatagtgata tggttgatcg tattttattc aataattcat taaaatatta tatgaacgaa
     9301 cacccagcag taacgcacac gacaattcaa ctcgtaaaag actatattat gtctatgcag
     9361 cattctgatt atgtatcgca aaacatgttt gacattataa atacagttga atttattggt
     9421 gagaattggg atagagaaat atacgaattg tggcgacaaa cattaattca agtgggcatt
     9481 aataggccga cttataaaaa attcttgata caacttaaag ggagaaagtt tgcacatcga
     9541 acaaaatcaa tgttaaaacg ataacgtgta cattgatgac cataaactgc aatcctatga
     9601 tgtgacaata tgaggaggat aacttaatga aacgtgtaat aacatatggc acatatgact
     9661 tacttcacta tggtcatatc gaattgcttc gtcgtgcaag agagatgggc gattatttaa
     9721 tagtagcatt atcaacagat gaatttaatc aaattaaaca taaaaaatct tattatgatt
     9781 atgaacaacg aaaaatgatg cttgaatcaa tacgctatgt cgatttagtc attccagaaa
     9841 agggctgggg acaaaaagaa gacgatgtcg aaaaatttga tgtagatgtt tttgttatgg
     9901 gacatgactg ggaaggtgaa ttcgacttct taaaggataa atgtgaagtc atttatttaa
     9961 aacgtacaga aggcatttcg acgactaaaa tcaaacaaga attatatggt aaagatgcta
    10021 aataaattat atagaactat cgatactaaa cgataaatta acttaggtta ttataaaata
    10081 aatataaaac ggacaagttt cgcagcttta taatgtgcaa cttgtccgtt tttagtatgt
    10141 tttattttct ttttctaaat aaacgattga ttatcatatg aacaataagt gctaatccag
    10201 cgacaaggca tgtaccacca atgatagtga ataatggatg ttcttcccac atacttttag
    10261 caacagtatt tgccttttga ataattggct gatgaacttc tacagttgga ggtccataat
    10321 ctttattaat aaattctctt ggatagtccg cgtgtacttt accatcttcg actacaagtt
    10381 tataatcttt tttactaaaa tcacttggta aaacatcgta aagatcattt tcaacataat
    10441 atttcttacc atttatcctt tgctcacctt tagacaatat ttttacatat ttatactgat
    10501 caaatgagcg ttccattaat gcattcccca tcatattacg ttgcttctcg ccaccaaggt
    10561 ttttatagtc tcctgcaccc atgataactt gattaattct aaatttacct cgtttggtag
    10621 taatcgtatg gttgtaattt gctgtatcac ttgatccagt ttttaaacca tctgtacccg
    10681 gcaaactcat ttttgcacct tccaatgaaa agttgaatgt gtaatacgta actgcatgcg
    10741 ttgttggtgc taactgcttt gtaaagtcta atattttagg tgtctcttta atcacgtgta
    10801 aatctaaaat ggcatagtct ctagcagtcg ttacagtacg ttcttggtct ttatactttg
    10861 ttggtgcaaa tgtacgtaat cttgaatttt cagcacccgt tggattgacg aaatgtgtat
    10921 ttttcattcc gatagcttta gctttgttat tcattaaatc aacgaaatcg ctggtgtttt
    10981 ttgaaacctt cttagctaaa attaatgccg cggcattact agaattagat actgtaattt
    11041 gtaataggtc tgcgattgtc catacttgtc caggatatag tttcgtatta ctcaactcag
    11101 gtagtgtaga cataatatat tctttgttcg tcattgtgac tgtgtcatca agtgaaagct
    11161 gccccttatt tacagcttcc aatgttaagt acattgtcat taatttagtc atagacgctg
    11221 gattccactt agtatcgata ttgtattgat acagtaattg tccagtttga cttacattaa
    11281 cagcactcgt cggttcgtat gcagccgaca aacctgcata accatattga tttgctgctt
    11341 gtacaggggt tacgtcactg ttagtagctt gtgcatatgg tgtcataata cttaatgtta
    11401 aacataaaat gatgataata gatattaaat ttttcataaa gcgttaatct tcccttttcc
    11461 aattcttaaa tattccctaa aagcaatggt tattcctact tacggaaatc attgctaatt
    11521 cacttcacct taattaaatt gttgaaaata aagttttctg cagttaattt gaaaaataat
    11581 gcaaatatat tacgtgtgta gctaaaggtg ttataatgtt tgtacgaaga gcaaacttac
    11641 tcaaaagcga ttaattttca tgttttaata taaagacttt gagaagttat tacaaaaaat
    11701 gcaatagaaa tattctatca tataaatgtt atgagcggta ttttggggca acactttatt
    11761 tgatttttaa agttttgttg ggagaaagta tatgatagaa atgcatgtat ctatctaaat
    11821 gaattaacta taaatttcaa acagaagagg taaaactatg aaacgagaaa atccattgtt
    11881 tttcttattt aaaaaactat catggccagt gggtcttatc gttgcagcta tcactatttc
    11941 atcactaggg agcttaagtg gactattagt gccactgttt actggacgaa ttgtagataa
    12001 attttccgtg agccatatca attggaatct aatcgcatta tttggtggta tctttgtcat
    12061 caatgcttta ttaagcggat taggtttata tttattaagt aaaattggtg aaaagattat
    12121 ttatgcgata cgctcagttt tatgggagca tatcatacaa ttaaaaatgc cattctttga
    12181 caaaaatgaa agtggtcaat taatgagtcg attaactgac gatacgaaag tgataaatga
    12241 atttatttca caaaagctac ctaacttatt accatcaatc gttacattag ttgggtcact
    12301 aatcatgtta tttattttag attggaaaat gacattatta acatttataa cgataccgat
    12361 attcgtttta attatgattc ctctaggtcg tattatgcaa aagatatcga caagtacaca
    12421 atctgaaatt gcaaacttca gtggtttgtt agggcgtgtc ctaactgaaa tgcgtcttgt
    12481 taaaatatca aatacagagc gtcttgaatt agataatgca cataaaaatt tgaatgaaat
    12541 atataaatta ggtttaaaac aggctaaaat tgcggcagtt gtacaaccaa tttcaggtat
    12601 agttatgttg ctaacaattg caattatttt aggttttggt gcattagaaa ttgcgactgg
    12661 tgcaatcact gcaggtacat taattgcaat gatattttat gttattcagt tatctatgcc
    12721 tttaatcaat ctttccacgt tagttacaga ttataaaaag gcagtcggtg caagtagtag
    12781 aatatacgaa atcatgcaag aacctattga accgacagaa gctcttgaag attctgaaaa
    12841 tgtattaatt gatgacggtg tattgtcatt tgaacatgta gactttaaat atgatgtgaa
    12901 gaaaatatta gatgatgtgt cgttccaaat cccacaaggt caagtgagtg cttttgtagg
    12961 cccttctggg tctggtaaaa gtacgatatt taatctgata gaacgtatgt atgaaattga
    13021 gtcaggtgat attaaatatg gccttgaaag tgtctatgat atcccgttat ctaagtggcg
    13081 acgcaaaatt ggatatgtta tgcaatcaaa ttcgatgatg agtggtacaa ttagagacaa
    13141 tattttatac ggaattaatc gtcatgtttc agatgaagaa cttattaatt atgctaaatt
    13201 agcgaactgt catgatttta tcatgcaatt tgatgaagga tatgacacgc ttgtaggtga
    13261 acgaggattg aaactgtctg gcggacaacg tcaacgtatt gatattgcta gaagttttgt
    13321 taaaaatcct gatattttgt tacttgatga agcaacagct aatctcgata gtgaaagtga
    13381 attgaaaatt caagaagctt tagaaacatt gatggaaggt agaacaacga ttgtcattgc
    13441 gcatcgtttg tctacaatta aaaaagccgg tcaaattata ttcttagaca aaggacaggt
    13501 aacaggtaaa ggtacgcatt cagaactgat ggcatcacat gcgaagtata aaaactttgt
    13561 agtgtctcaa aaattaacag attaatttta tatatataag taagcttgga gcaaatacac
    13621 atataccatc gaggaaatta aagtgtggca cattgatgga tatagatgtt aataaattgc
    13681 ttcaagcttt tgtctatttt aaatcatttg agaagttacg acataataat tcttaaatta
    13741 atgaaatcga tattttaaga aaaaaatgct catggtataa ta
//
LOCUS       DANRDI010000064        13514 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.064, whole genome shotgun sequence.
ACCESSION   DANRDI010000064 DANRDI010000000
VERSION     DANRDI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.064"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..693
                     /locus_tag="PZ377_001444"
     CDS             <1..693
                     /locus_tag="PZ377_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358318.1"
                     /translation="SDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQA
                     ERLANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVN
                     IDNKDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTN
                     KTSGQKKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEK
                     AKSKIEKRTFND"
     gene            828..932
                     /locus_tag="PZ377_001445"
     CDS             828..932
                     /locus_tag="PZ377_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358319.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
     gene            1393..2484
                     /locus_tag="PZ377_001446"
     CDS             1393..2484
                     /locus_tag="PZ377_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDM6358320.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
     gene            2838..2951
                     /locus_tag="PZ377_001447"
     CDS             2838..2951
                     /locus_tag="PZ377_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358321.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            3024..4013
                     /gene="ccpA"
                     /locus_tag="PZ377_001448"
     CDS             3024..4013
                     /gene="ccpA"
                     /locus_tag="PZ377_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDM6358322.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(4111..5280)
                     /locus_tag="PZ377_001449"
     CDS             complement(4111..5280)
                     /locus_tag="PZ377_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDM6358323.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(5305..5937)
                     /locus_tag="PZ377_001450"
     CDS             complement(5305..5937)
                     /locus_tag="PZ377_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDM6358324.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            6106..7812
                     /gene="acsA"
                     /locus_tag="PZ377_001451"
     CDS             6106..7812
                     /gene="acsA"
                     /locus_tag="PZ377_001451"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDM6358325.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            8252..9919
                     /locus_tag="PZ377_001452"
     CDS             8252..9919
                     /locus_tag="PZ377_001452"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDM6358326.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            10214..12901
                     /gene="harA"
                     /locus_tag="PZ377_001453"
     CDS             10214..12901
                     /gene="harA"
                     /locus_tag="PZ377_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HDM6358327.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            13032..13124
                     /locus_tag="PZ377_001454"
     CDS             13032..13124
                     /locus_tag="PZ377_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358328.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(13104..>13514)
                     /locus_tag="PZ377_001455"
     CDS             complement(13104..>13514)
                     /locus_tag="PZ377_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDM6358329.1"
                     /translation="YSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISG
                     IASASYSLFGIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHY
                     VLKRMLIEDQITEKEYNDALKYDIKSHLLNRKKR"
BASE COUNT         5022 a         1960 c         2472 g         4060 t
ORIGIN      
        1 agtgatatat cacaagaggc acaagaggtt caagaagcta aaaaagaagc acaagcagag
       61 aaagacagtg acacattaac taaagatgca agtgcagcaa aggtagaagt atcaaaacca
      121 gagtcacaag ctgaaagatt agcaaacgct gcaaaacaga agcaagctaa attaacacca
      181 ggttcaaaag agagtcaatt aactgaagcg ttatttgcag aaaaaccagt tgctaaaaat
      241 gacttgaaag aaattcctca attagttact aaaaagaatg atgtatcaga gacagagacg
      301 gttaatatag ataataaaga cactgttaaa caaaaagaag ctaaatttga aaatggtgtt
      361 attacacgta aagctgatga aaaaacaact aataatacag ctgttgacaa gaaatcaggt
      421 aaacaatcta aaaaaacaac accttcaaat aaacgaaatg catcaaaagc atctacaaat
      481 aaaacttcag gtcagaaaaa gcaacataat aagaaatcat cacaaggtgc aaagaaacaa
      541 agtagttcaa gtaagtcaac tcaaaagaat aatcaaacta gtaataagaa ttcaaaaaca
      601 acaaatgcta aatcatccaa tgcatcaaaa acgccaaatg ctaaagttga gaaagctaaa
      661 agtaaaatag agaaacgtac attcaatgac taagattact aaaatcgttg aataatcaat
      721 catagcagta agctatgtaa tatattttta aaaattgaat ttcaaaataa aaggtaggca
      781 ttcaggtgct tacctttttt gatatcatca gtttattttg aaaggcaatg cgatcatttc
      841 atgtatttat gttgtttgaa acatcgttag ataacaatag tgatattgca cattttaagc
      901 tgaagatgga aaaatattcg ataatcaaat aaacaatgaa ttttagaagg tacaatgacg
      961 ttcactaatt taaatatagc tgaatgtgtt ggtgagtgat gttcactata gatttatatt
     1021 aatatacaaa gacaaaggtt gttaattttt attaagcgtt aggttgaatg tatgagaatt
     1081 ttagatttat aatagaagat agaaacgaaa atttttctta aaagcagtaa tgttgactca
     1141 aaataagcta taataatgac acttatttaa ttgattaaca tttgctaata aatatcaata
     1201 tagaatataa ctttccaata atgactgaga aaatcgaaat gtcagtctcg aatcatataa
     1261 ttagaaaatt gattattttc tgtcaattta gggttgaact atacatatga tattgttaga
     1321 atatttttta acattatatt ttattgcttt aaagtggaat atacttgaaa taattagtag
     1381 aggtgagtaa ggatgagtaa taaattagaa tcatacagaa gtgagattgt atcactgaat
     1441 catcaaattt tagacttatt atctaaacgt ggtgaactag cacaaaaaat tggggaagaa
     1501 aaattaaaac aaggtacacg tatttatgat ccacaacgtg aaaaagaaat gcttaacgac
     1561 ttaatcgata gtaacaaagg accattcaac gataatacta ttaagcaatt atttaaagaa
     1621 attttcaaag cctctacaga tttacaaaaa tctgaaaatg aaaaacattt atatgtatca
     1681 cgtaagttga aacctgaaga tacgattgta acatttgata atgggggcat tattggagac
     1741 ggcaataaat catttgtatt tgggccatgt tcagttgaat catttgaaca agttgaagct
     1801 gttgctaaaa acttacatgc taaaggtgaa aaatttattc gtggcggtgc atttaaacca
     1861 cgtacatcac catatgattt ccaaggccta ggtgttgaag gacttaaaat acttaaacag
     1921 attaaagata aatatgattt aaatgttgtc agcgaaatcg taaatccaaa tgattttgaa
     1981 gtggctgatg agtatttaga cgtattccaa attggtgcac gtaatatgca aaacttcgag
     2041 ttattaaaag aagctggccg tacgaaaaag cctattctat taaaacgtgg tttatctgct
     2101 acaatcgaag agtttgttta tgcagctgaa tacattgctt cacaaggtaa tcaaaacatt
     2161 attttatgtg aacgtggaat ccgaacttat gaaaaggcga cacgtaacac tttagatatt
     2221 tcagcagtac caattttaaa acaaggtaca cacttaccag tcatggtaga tgttacgcat
     2281 agtacaggtc gtaaagatat catgttacca actgcgaaag cagcattagc agttggtgct
     2341 gatggagtta tggctgaggt gcatccagat ccatctgttg cacttagtga tgcgggtcaa
     2401 caaatggatt tagatgaatt ccaagcattt tatgatgaat taaagccttt agctgattta
     2461 tataacgcta aaaagttaaa ataatattcc aaggaaacta tagactactt aactaatatg
     2521 tcatgttgaa gtagaatatt atctttgaat cgacaatttt aaacttacag ccattctaag
     2581 agtatattac ttttagagtg gctattattt tttgtataga aataaaggta tactgcactt
     2641 aacgattgtt ataatacttc gacacttgtt caatttcaca attattaaag attatgactg
     2701 atagcagtaa ttaaaattat aactatgaat tatctgtaaa atataataga ttcacacatt
     2761 tgttgctgaa atgtgaacat ttttcaacaa atgcaattga tatttgaaaa ggctttctca
     2821 aaacattaca attaaaaatg aaaaaagttt atataaaatt aaaatatatc gttcgttatc
     2881 atttagcgtt tgtttttatt tcaagctttt cgctaaattt ttccaaacaa aaatatgtta
     2941 ctgtaaatta aaatatggta aactatgaaa atgaaatgaa aacatgttat tataatgaat
     3001 aaaacgttta caaggaggaa attatgacag ttactatata tgatgtagca agagaagcgc
     3061 gtgtctctat ggccacagtg tcgcgtgttg ttaatgggaa ccaaaatgtt aaagcagaaa
     3121 ctaaaaataa agttaacgaa gtcattaagc gtttgaatta tcgtccaaat gctgttgcta
     3181 gaggtttagc tagtaaaaag acaacaacag taggtgtgat cattccagat atatctaata
     3241 tctattattc acaacttgct cgtggacttg aagatattgc aacaatgtat aaatatcact
     3301 caattatttc aaattcagat aacgatcctg aaaaggaaaa agaaattttt aataacttat
     3361 taagtaaaca ggttgatggt attattttcc ttggtggtac aattactgaa gaaatgaaag
     3421 aattgataaa tcaatcatct gtacctgtag tagtatcagg aacaaatggt aaggatgcac
     3481 atatagcatc agttaatatt gattttactg aagctgcgaa agaaattacg ggagaattaa
     3541 ttgaaaaagg cgctaaatca tttgctttag taggtggaga acattctaaa aaagctcaag
     3601 aagatgtttt agaaggttta actgaagtgt taaataaaaa tggccttcaa ttaggtgata
     3661 cattgaattg ttctggtgct gaaagttata aagaaggcgt aaaagctttt gccaaaatga
     3721 aaggcaattt gccagatgcc attttatgta tcagcgacga agaagcaatt ggtattatgc
     3781 atagtgcaat ggatgctggt attaaagttc cagaggaatt acaaattatt agtttcaata
     3841 atacacgatt agttgagatg gttagaccac aactttctag tgttattcaa ccattatatg
     3901 atatcggtgc agtagggatg cgcttattaa caaaatatat gaacgatgaa aagatagaag
     3961 aaccaaatgt agttttacct cacagaattg aataccgagg aactacaaaa taaattcaca
     4021 aaattaggca ttcatctaac gacccaaatt atatgggtgt tggaagaatg ccttttattt
     4081 atcttttaaa atcgttgcag attaggttac ttattgacga gtagattcgt accaactcgc
     4141 tatatgtaaa gctaattttt tatttttttc actaatttct tttgtgcggg ggacataggt
     4201 ataatcattt aaacgatctt cccatctttt aggtaataat tcagatgaat aatgtttcca
     4261 tttattaatc cattctaacg gtaaataacc actttgaatt ggttgatcaa ttaaacttaa
     4321 gaatacatga ctccatgcac gtggtacgac tctccaaata ttgtagcctc cgccaccaaa
     4381 cataattacc tttccattcg tataagaatc agctaaatat tttacaaaat atggaatttc
     4441 atataatgaa tgtaacgtac aatttagatg agttagtgga tcacgataat gtatatcgac
     4501 accatttacg cttagaataa tatcaggttt aaaactcttt acgacaggct caactgttaa
     4561 tttaaaacac tccaaaaatg atgcatcttc tgtatacggt tcaagtggga catttacagt
     4621 gtgtccatag ccgatatctt caccgcgctc agtatagtga ccagagcctg ggaaaagaaa
     4681 ttttccggtt tcatggatag aataagtagt aacatggtta tcggcataga aactccattg
     4741 tgtaccatct ccatgatgtg catcggtatc tatgattaaa acgcgttgat tgtattcttt
     4801 agctaagtat tgtgcggtaa ttgcaatatc attgtatata caaaaaccac ttgctcgacc
     4861 aggttgagcg tgatgcaaac caccacctaa gtgacaacca tttaatactt tgcctgacat
     4921 aataagatct gctaaagtta aagcgcctcc aacaattgtg gcactatggc ggtgcatatg
     4981 cttaaattga ccattctctt catcatttaa tccatatttc ttagcctcat cttcactgat
     5041 aatgccatgt gaagcatgct taatagcttc gacgtaatca tatttatgaa ttaacattaa
     5101 ttcgtcatct gttgcaattc taggttgtac tatttgttct ggagacaata aatttgcatt
     5161 caaaagtagc tctgttgtta attttaaacg catttgattg aagggatgtt ggtcatgaaa
     5221 tcgatattgt aataacttat ctgaataaac atatgcagtt tttgatgaat gttgttgcat
     5281 ataatccctc cgatattcca aaaattaaaa gaaaaaccga ttcatataac gaatatcatc
     5341 aaacgcttgt tgctgttcta atgtaatgtt tttgccaatt cttgccatta aacaattagc
     5401 tggatgactt gttatttctg gatcatctgt agcgaatatt tcaagtccac cagttgccat
     5461 taaccgctgc attaattttt tatagtcaaa tacatctaac tttgaatttt ttaaatccca
     5521 atgccagtaa tattctgtag ttataacgat ataattctcg aattctggtg tagaaaggct
     5581 aagttgtatc agcttttctg caagttgtaa ttgcctaaaa ttgatgctga cttcaattgc
     5641 ccccaattcg attaaatatg gaagcttacc tgtagaccag cgctcaattt catcaggata
     5701 gtggaaagtg acataaccca caatgagttg atcttggcga gcaacataaa ttctaccttc
     5761 tggtaatgtt gtaatttcta acaaagcttt atactgatct tcagcatctc taaatgcggt
     5821 taaatgcgca tcgaaagtaa gcgctttcaa atcttcgtgt gttaaaggac cttcaataac
     5881 aaattgcttg tcatgaatgt aataatcttc ggattgatac gtctttaaat gattcatatt
     5941 ttcaactcct caatcgacgt cgtgattgta ttaaattcat tatatagaaa atttacaata
     6001 attaatacta gaaaaaggat aaaagtaaaa attttgaata attagaaatg ttatgtataa
     6061 tattgagaaa gaaagcgttt tcacataaca aagggggagt ttcaaatgaa agtcgaagtt
     6121 tataaaggag cgcaaggtaa acataacctt aaagattatg aagaaacata taatactttt
     6181 gattggaaag acgtagaaca agcattttct tggagtgaaa ctggaaaaat gaacatggca
     6241 tatgaatgca tagatcgcca tgtagatcaa ggattagggg ataaaatagc gttaaattac
     6301 aaagatgagc acagaaaaga atcgtatact tataaagata tgcaacggtt atctaataaa
     6361 gcagcgaatg ttttgtctga acatgcagaa gttgacaaag gtgacagagt atttatattt
     6421 atgtcgcgta cacctgaact atattttgcg ttgttaggtg ttttaaaaat tggtgcaatt
     6481 gttgggccgt tatttgaagc atttatggaa aaggcagttg cggatagatt agagaacagt
     6541 gaagctaaag tgttaattac taataaggca ttgttacctc gagtacctgt agataaatta
     6601 ccaaacttga aaaaaattgt tgtcgtagat gaggatgtag aagacaatta catagacttc
     6661 attagtttga tggaaactgc tagcgatgaa tttgacattg aatggttaaa gtcggatgat
     6721 ggtttgattt tacattatac atcaggttct actgggcaac ctaaaggtgt attgcatgtt
     6781 caacaagcaa tgttagtgca ctatatttct ggaaaatatg tattagattt acaagaagat
     6841 gatgtttatt ggtgtacagc agatccaggt tgggttacag gaacatctta tggtattttt
     6901 gcaccatggt taaatggcgc tacaaattgt atagctggtg gtcgcttttc gccagaacag
     6961 tggtatagta tgattgaaga ttttaaagtg acgatttggt atacggcacc aacagcttta
     7021 agaatgttaa tgagtgctgg tgacgatatt gttgagaaat atgacttgtc atcgttacgt
     7081 tcgattctat cagtaggtga gcctttaaat cctgaagtta taaaatgggc gaaaaaagta
     7141 tacggtttaa cggtgttaga tacttggtgg atgacagaaa caggtggaca tatgattgtt
     7201 aactatccaa cgatggacgt caagcttggc tcaatgggca aaccattacc tggtattcaa
     7261 gctgcaatta tcgatgatgc agggaatgaa ttaccaccaa atcgaatggg caaccttgct
     7321 ataaaaaaag gctggccatc aatgatgtat cgtatctgga agaatccaga aaaatataaa
     7381 tcatatttta ttggagactg gtatgtatct ggtgattcgg catataaaga tgaagatggt
     7441 tacttctggt tccaaggacg tgttgatgat gtaattatga cagctggtga acgagttgga
     7501 ccatttgagg ttgagtctaa attggttgaa cacgaagcag ttgccgaagc aggaattatt
     7561 ggtaaacctg atccggttcg cggtgaaata attaaggcgt ttgttgcact gagaaaagga
     7621 tatgaaccaa cagacgaatt aaaagaagaa attcgtatat ttgttaaaga aggtttgtcg
     7681 gcacatgcag caccacgtga aatcgaattt aaagataaat tacctaaaac acggtcaggt
     7741 aaaattatga gacgtgtatt aaaagcttgg gaattaaatt tagatgctgg ggatttaagt
     7801 acaatggaat aatgacatga atgttattga agattttttt cgaagaataa agggtgacaa
     7861 catatttcat gtcaatgttt aaataatcgt ttactttacg ataagcaata taaagaactg
     7921 ttaacttgtg tcatatcatt tcgtagaaag catttgaaaa tgatgacata acaataatgg
     7981 catatcttta tattgctttt tatttttaat atgatctttg gaagatgatt attttaaata
     8041 atagaaaaat atagttatca atagtatcaa gcgctaaaag ttgtataata caaaacttta
     8101 ataagtgaat ttattgcaaa aatgaaagcg ctaacccgat ttagtcgaca agtttttaac
     8161 agttcgttat tatatgaatg taagtaaaaa tttcttagct acaacttaca tattataaat
     8221 gcataaatta aacaaaaagg ggcgaaaaaa gttgactcat ttatcagatt tagatattgc
     8281 gaatcaatca acactacaac caattaagga tattgctgca tcagtaggta tttcagagga
     8341 tgcattagaa ccttatggtc attacaaagc taaaatcgac attaataaaa ttacgccaag
     8401 agaaaacaaa gggaaagttg ttttagtaac tgcgatgagc ccaacaccag ctggtgaagg
     8461 taaatcaacg gttacagttg gtttagctga tgcattccat gagttaaata aaaacgttat
     8521 ggttgcatta agagagcctg ctttaggacc aacatttggt atcaaaggtg gtgcgactgg
     8581 tggtggttat gcgcaagtct tacctatgga agatatcaac ttacatttca acggagattt
     8641 ccatgcgatt acaactgcaa ataatgcatt gtctgcgttt atcgataatc atattcacca
     8701 aggtaacgaa ttaggaatcg atcaaagacg tattgagtgg aaacgtgtat tagatatgaa
     8761 tgatcgtgca cttagacatg taaacgttgg gttaggtgga cctacaaatg gtgtaccacg
     8821 tgaagatggc tttaatatta cagtagcgtc tgaaattatg gcgattttat gtttaagtag
     8881 aagtattaaa gacttaaaag ataaaattag tcgtattact attggttaca ctagagatcg
     8941 caagccagtt acagttgcag atttaaaagt ggaaggtgca cttgcaatga ttttaaaaga
     9001 tgcaataaaa ccaaacttag tacaatcaat tgaagggaca cctgcattag ttcatggtgg
     9061 accatttgcg aatatcgcac acggttgtaa ctcaatttta gcaactgaaa cagcacgtga
     9121 tttagctgat atcgttgtaa cggaagctgg atttggttca gacttaggcg ctgaaaaatt
     9181 catggacatt aaagtgcgtg aagcaggatt tgatccggca gctgtcgttg ttgttgcgac
     9241 aattcgtgcg ttaaaaatgc atggtggtgt agcgaaagat aatttaaaag aagaaaatgt
     9301 agaagcagta aaagcaggaa ttgttaattt agagcgtcat gttaataata ttaaaaaatt
     9361 cggtgtagaa ccggttgttg caattaatgc atttatacat gataccgatg cagaagtaga
     9421 atatgtaaaa tcttgggcta aagaaaataa cgtacgaatt gccttaactg aagtttggaa
     9481 aaaaggtggt aaaggtggcg ttgacttagc aaatgaagta ttagaagtca ttgatcaacc
     9541 taattcattt aaacctttat atgaattaga attaccatta gagcaaaaga ttgaaaagat
     9601 tgtgactgaa atctatggcg gttcaaaagt aacgtttagc agtaaagcgc aaaaacaatt
     9661 aaaacaattt aaagaaaatg gttgggataa ttacccagta tgtatggcga aaacacaata
     9721 ttcattctca gatgatcaaa cgttgttagg tgcaccatca ggatttgaaa ttacaattcg
     9781 tgaattagaa gcgaaaacag gtgcaggatt tatcgtagcg ttgacaggtg caatcatgac
     9841 tatgcctggt ttacctaaaa aaccagcagc attaaacatg gatgttactg atgatggtca
     9901 tgcaattggg ttattctaat aaatcatgtc aattgtttaa taaagataag taaatagttt
     9961 aatagaccgg actgttggag atgcattatt tcagcagttc ggttttttgc tgtgctaaaa
    10021 atagattcaa tttggcgaat ctaacgacaa tgtttgaagg tggttaatta atgtatatga
    10081 agataaaaag tgggcttgaa gaataggaaa gcgatgcaat gaatattcca tattaaaaaa
    10141 aataataaaa taggttgcaa tatttaattg ggatgcgcta caattaacac taataatagg
    10201 agttgttaac aacatgaaca aacatcaccc aaaattaagg tctttctatt ctattagaaa
    10261 atcaactcta ggcgttgcat cggtcattgt cagtacacta tttttaatta cttctcaaca
    10321 tcaagcacaa gcagcagaaa atacaaatac ttcagataaa atctcggaaa atcaaaataa
    10381 taatgcaact acaactcagc cacctaagga tacaaatcaa acacaacctg ctacgcaacc
    10441 agcaaacact gcgaaaaact atcctgcagc ggatgaatca cttaaagatg caattaaaga
    10501 tcctgcatta gaaaataaag aacatgatat aggtccaaga gaacaagtca atttccagtt
    10561 attagataaa aacaatgaaa cgcagtacta tcactttttc agcatcaaag atccagcaga
    10621 tgtgtattac actaaaaaga aagcagaagt tgaattagac atcaatactg cttcaacatg
    10681 gaagaagttt gaagtctatg aaaacaatca aaaattgcca gtgagacttg tatcatatag
    10741 tcctgtacca gaagaccatg cctatattcg tttcccagtt tcagatggca cacaagaatt
    10801 gaaaattgtt tcttcgactc aaattgatga tggagaagaa acaaattatg attatactaa
    10861 attagtattt gctaaaccta tttataacga tccttcactt gtaaaatcag atacaaatga
    10921 tgcagtagta acgaatgatc aatcaagttc agtcgcaagt aatcaaacaa acacgaatac
    10981 atctaatcaa aatacatcaa cgatcaacaa tgctaataat caaccgcagg caacgaccaa
    11041 tatgagtcaa cctgcacaac caaaatcgtc aacgaatgca gatcaagcgt caagccaacc
    11101 agctcatgaa acaaattcta atggtaatac taacgataaa acgaatgagt caagtaatca
    11161 gtcggatgtt aatcaacagt atccaccagc agatgaatca ctacaagatg caattaaaaa
    11221 cccggctatc atcgataaag aacatacagc tgataattgg cgaccaattg attttcaaat
    11281 gaaaaatgat aaaggtgaaa gacagttcta tcattatgct agtactgttg aaccagcaac
    11341 tgtcattttt acaaaaacag gaccaataat tgaattaggt ttaaagacag cttcaacatg
    11401 gaagaaattt gaagtttatg aaggtgacaa aaagttacca gtcgaattag tatcatatga
    11461 ttctgataaa gattatgcct atattcgttt cccagtatct aatggtacga gagaagttaa
    11521 aattgtgtca tctattgaat atggtgagaa catccatgaa gactatgatt atacgctaat
    11581 ggtctttgca cagcctatta ctaataaccc agacgactat gtggatgaag aaacatacaa
    11641 tttacaaaaa ttattagctc cgtatcacaa agctaaaacg ttagaaagac aagtttatga
    11701 attagaaaaa ttacaagaga aattgccaga aaaatataag gcggaatata aaaagaaatt
    11761 agatcaaact agagtagagt tagctgatca agttaaatca gcagtgacgg aatttgaaaa
    11821 tgttacacct acaaatgatc aattaacaga tttacaagaa gcgcattttg ttgtttttga
    11881 aagtgaagaa aatagtgagt cagttatgga cggctttgtt gaacatccat tctatacagc
    11941 aactttaaat ggtcaaaaat atgtagtgat gaaaacaaag gatgacagtt actggaaaga
    12001 tttaattgta gaaggtaaac gtgtcactac tgtttctaaa gatcctaaaa ataattctag
    12061 aacgctgatt ttcccatata tacctgacaa agcagtttac aatgcgattg ttaaagtcgt
    12121 tgtggcaaac attggttatg aaggtcaata tcatgtcaga attataaatc aggatatcaa
    12181 tacaaaagat gatgatacat cacaaaataa cacgagtgaa ccgctaaatg tacaaacagg
    12241 acaagaaggt aaggttgctg atacagatgt agctgaaaat agcagcactg caacaaatcc
    12301 taaagatgcg tctgataaag cagatgtgat agaaccagag tctgacgtgg ttaaagatgc
    12361 tgataataat attgataaag atgtgcaaca tgatgttgat catttatccg atatgtcgga
    12421 taataatcac ttcgataaat atgatttaaa agaaatggat actcaaattg ccaaagatac
    12481 tgatagaaat gtggataaag atgccgataa tagcgttggt atgtcatcta atgtcgatac
    12541 tgataaagac tctaataaaa ataaagacaa agtcatacag ctgaatcata ttgccgataa
    12601 aaataatcat actggaaaag cagcaaagct tgacgtagtg aaacaaaatt ataataatac
    12661 agacaaagtt actgacaaaa aaacaactga acatctgccg agtgatattc ataaaactgt
    12721 agataaaaca gtgaaaacaa aagaaaaagc cggcacacca tcgaaagaaa acaaacttag
    12781 tcaatctaaa atgctaccaa aaactggaga aacaacttca agccaatcat ggtggggctt
    12841 atatgcgtta ttaggtatgt tagctttatt cattcctaaa ttcagaaaag aatctaaata
    12901 attaactaaa tatagcatat gtatgattaa ctttgtagac aatgtgaaag caattaattt
    12961 ataaactatt gattggttta atggctttcc tttagagtaa ataaaaagaa cagcagtgag
    13021 aaattttcta attgaaaata atcttactgc tgtttttaat atttggattc attgttgtgg
    13081 ttactttaaa aagtgagcat caattaacgc ttttttcgat ttaacaaatg tgatttaata
    13141 tcatatttta atgcgtcgtt gtattctttt tcagtgattt gatcttcgat taacatacgc
    13201 tttaatacat aatgttgtct ttgaatacta tatttcaaat ctttatccga ttttaacgtt
    13261 ccatcttttt cgtagggtgt atagccatat gggctttgca acaaaccgat aaggtatgca
    13321 gattgtgcaa ttgataaatc ttttggtgga ataccaaata gactatatga agcggatgca
    13381 attccggaaa tattagcgcc attataatct ctaccgaagg gaactatatt taaatatgta
    13441 tatataattt catcttttga gagtaggtgt tctaatctaa ttgctaggcg aagttcattt
    13501 gcttttctac tata
//
LOCUS       DANRDI010000065        13444 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.065, whole genome shotgun sequence.
ACCESSION   DANRDI010000065 DANRDI010000000
VERSION     DANRDI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.065"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            70..1263
                     /locus_tag="PZ377_001456"
     CDS             70..1263
                     /locus_tag="PZ377_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDM6358330.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            1260..1523
                     /locus_tag="PZ377_001457"
     CDS             1260..1523
                     /locus_tag="PZ377_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDM6358331.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            1541..1933
                     /locus_tag="PZ377_001458"
     CDS             1541..1933
                     /locus_tag="PZ377_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDM6358332.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            2038..2439
                     /locus_tag="PZ377_001459"
     CDS             2038..2439
                     /locus_tag="PZ377_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDM6358333.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            2619..3914
                     /gene="tilS"
                     /locus_tag="PZ377_001460"
     CDS             2619..3914
                     /gene="tilS"
                     /locus_tag="PZ377_001460"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDM6358334.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            3919..4458
                     /gene="hpt"
                     /locus_tag="PZ377_001461"
     CDS             3919..4458
                     /gene="hpt"
                     /locus_tag="PZ377_001461"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDM6358335.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            4715..6808
                     /gene="ftsH"
                     /locus_tag="PZ377_001462"
     CDS             4715..6808
                     /gene="ftsH"
                     /locus_tag="PZ377_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDM6358336.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
     gene            7037..7918
                     /gene="hslO"
                     /locus_tag="PZ377_001463"
     CDS             7037..7918
                     /gene="hslO"
                     /locus_tag="PZ377_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDM6358337.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            8097..9029
                     /gene="cysK"
                     /locus_tag="PZ377_001464"
     CDS             8097..9029
                     /gene="cysK"
                     /locus_tag="PZ377_001464"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDM6358338.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            9245..10048
                     /gene="folP"
                     /locus_tag="PZ377_001465"
     CDS             9245..10048
                     /gene="folP"
                     /locus_tag="PZ377_001465"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDM6358339.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            10026..10391
                     /gene="folB"
                     /locus_tag="PZ377_001466"
     CDS             10026..10391
                     /gene="folB"
                     /locus_tag="PZ377_001466"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDM6358340.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            10388..10864
                     /gene="folK"
                     /locus_tag="PZ377_001467"
     CDS             10388..10864
                     /gene="folK"
                     /locus_tag="PZ377_001467"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDM6358341.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            11258..11350
                     /locus_tag="PZ377_001468"
     CDS             11258..11350
                     /locus_tag="PZ377_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358342.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            11403..12890
                     /gene="lysS"
                     /locus_tag="PZ377_001469"
     CDS             11403..12890
                     /gene="lysS"
                     /locus_tag="PZ377_001469"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDM6358343.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
BASE COUNT         4900 a         1739 c         2740 g         4065 t
ORIGIN      
        1 gacatataaa gagttaactt atttaccatt tggttcaaag ttgtatcaaa ttaagaaagg
       61 aagacgttga tggcacatac cattacgatt gttggcttag gaaactatgg cattgatgat
      121 ttgccgctag ggatatataa atttttaaag acacaagata aagtttatgc aagaacgtta
      181 gatcatccag ttatagaatc attgcaagat gaattaacat ttcagagttt tgaccatgtt
      241 tatgaagcac ataaccaatt tgaagatgtc tatattgata ttgtggcgca attggttgaa
      301 gctgctaatg aaaaagatat tgtctatgcg gttccgggtc atcctagagt tgctgagaca
      361 actacagtga aattactggc tttagcaaag gacaatactg atatagatgt gaaagtttta
      421 ggtgggaaaa gctttattga tgatgtgttt gaagcagtta atgtagatcc aaatgatggc
      481 ttcacactgt tagatgcgac atcattacaa gaagtaacac ttaatgttag aacgcataca
      541 ttgattacgc aagtttatag tgcaatggtt gctgctaatt tgaaaatcac tttaatggaa
      601 cgatatcctg atgattaccc tgttcaaatt gtcactggtg cacgaagcga tggtgcggat
      661 aacgttgtga catgcccatt atatgaattg gatcatgatg aaaatgcatt caataatttg
      721 acgagtgtat tcgtaccaaa aatcataaca tcgacatatt tgtatcatga ctttgatttt
      781 gcaacggaag tgattgatac tttagttgat gaagataaag gttgtccatg ggataaagtg
      841 caaacgcatg aaacgctaaa gcgttattta cttgaagaaa catttgaatt gttcgaagct
      901 attgacaatg aagatgattg gcatatgatt gaagaactag gagatatttt attacaagtg
      961 ttattgcata ctagtattgg taaaaaagaa gggtatatcg acattaaaga agtgattaca
     1021 agtcttaatg ctaaaatgat tcgtagacac ccacacatat ttggtgatgc caatgctgaa
     1081 actatcgatg acttaaaaga aatttggtct aaggcgaaag atgctgaagg taaacagcca
     1141 agagttatat ttgaaaaagt atttgcagag cattttttaa atttatatga gaagacgaag
     1201 gataagtcat ttgatgaggc cgcgttaaag cagtggctag aaaaagggga gagtaataca
     1261 tgagattaga taaatattta aaagtatcac ggttaataaa gcgacgtacg ctagcaaaag
     1321 aagtaagtga tcaaggtaga attacaataa atggtaatgt tgctaaagct ggatcggatg
     1381 ttaaagttga agatgtgctg acgattcgct ttggtcaaaa attagtaaca gttaaagtaa
     1441 ctgcattaaa tgaacatgca tctaaagata acgcgaaggg tatgtatgaa atcattgaag
     1501 agcgtcgact tgaagaagcg taaattggag gtgacaagca atgaaaaata aagtagaaca
     1561 tatagaaaat cagtacacgt cgcaagagaa caagaaaaaa caacgtcaaa aaatgaaaat
     1621 gcgtgttgtt cgtaggcgta ttacagtatt tgcgggcgta ttacttgcga taattgttgt
     1681 tttatcaatc ttgcttgttg tccaaaaaca tcgcaatgat attgatgcac aggagcgaaa
     1741 agcgaaagaa gcacagtttc aaaagcaaca aaatgaagaa attgcgttaa aagaaaagtt
     1801 gaataatctg aatgacaaag attacattga aaaaattgcg cgtgatgatt attacttaag
     1861 caacaaaggt gaagtgattt ttaggttgcc agaagacaaa gattcgtcta gctcaaaatc
     1921 ttcgaaaaaa taaatccaaa ttgattcaaa attatccgag tatagacatt gtgaaaaaat
     1981 ccaaacaagg atataataag ggaaaatcga atcaaatcgg gaggatttat ttaacatatg
     2041 tcaatcgaag ttggaaataa gcttaaaggt aaagtcactg gtattaaaaa gtttggtgca
     2101 ttcgtagaat tacctgaagg aaaaagtggt ttagttcaca ttagtgaagt cgcagataat
     2161 tatgttgaaa acgtagaaga gcacctttct gttggtgatg aagtagacgt aaaagtatta
     2221 tctattgctg atgatggaaa aattagtctt tcaattaaga aagctaaaga ccgtccacgt
     2281 agacaacata cgagtaaacc aagtcatcaa aaaccagtgc aaaaagccga agattttgaa
     2341 aagaaattaa gcaatttctt aaaagatagt gaagataaat taacttcaat caaacgtcaa
     2401 acagaatcta gacgcggtgg caaaggttca agacgttaat taaaataaat aaagactgct
     2461 tcgataagga atatatttag aatgatgcgt atcgaataat cgattgcagc gttagacaat
     2521 ctaagactgt ttcttaaata aggagcagtc tcttttattt gtaatgatat aactaagact
     2581 tataccattt ttgaaaattg taaaagtgag gtgatgttat gcagttaaat agtaatggtt
     2641 ggcatgttga tgaccatatt gttgtcgctg tttctacagg tattgatagt atgtgtttat
     2701 tgtatcaact actaaatgat tataaagata gttatagaaa actaacatgc ttacatgtca
     2761 atcatggcgt taggtcagct tcaattgagg aagccagatt tttagaagca tactgcgaac
     2821 gtcatcacat cgatttacat atcaaaaagt tagatttgtc gcatagtctc gaccgaaata
     2881 acagcattca gaatgaagct cgaattaaac gttacgaatg gtttgatgaa atgatgaatg
     2941 tattagaagc ggatgtattg ctaacggcgc atcatttgga cgatcaatta gaaactatta
     3001 tgtatcgtat ttttaatggg aaatcaacac gtaataaact aggatttgat gagttatcga
     3061 agcgaaaagg ttatcagatt tatcgaccac ttttagctgt ctctaaaaaa gaaataaaac
     3121 aattccaaga gagatatcat attccatatt ttgaagatga atctaataaa gataacaaat
     3181 atgttagaaa tgatattcgt aatagaatta ttccagctat tgatgaaaat aatcaactta
     3241 aagtatcgca tttattaaaa ttaaaacaat ggcatgatga acaatatgat attttgcaat
     3301 attcagctaa acaatttatt caagaatttg tgaagtttga tgaacagtca aaatatttag
     3361 aggtttctag acaagctttt aataacttac caaactcatt aaagatggtt gtgttggact
     3421 gcctattatc aaagtattat gagttgttta atattagtgc taaaacatac gaagagtggt
     3481 ttaaacaatt tagtagtaag aaagcacaat tcagtattaa tctcacggat aaatggataa
     3541 ttcaaatcgc atatggtaaa ttaataataa tggctaaaaa taatggcgat acatatttta
     3601 gagttcaaac aattaaaaag ccaggtaatt atatttttaa caaatatcga ttagagatac
     3661 attctaattt accaaaatgt ttatttccgc ttacagtgag aacacgacaa agtggcgata
     3721 catttaaact gaatgggcgc gatggttata agaaagtgaa tcgcctgttt atagattgta
     3781 aagtgccaca gtgggttcgg gatcaaatgc caatcgtatt ggataaacaa cagcgcatta
     3841 ttgcggtagg agatttatat caacaacaaa caataaaaaa atggattata attagtaaaa
     3901 atggagatga atagcgttat gcataatgat ttgaaagaag tattgttaac tgaagaagat
     3961 attcaaaata tctgtaagga attgggagca caattaacaa aggattatca aggtaaacca
     4021 ttagtatgcg tgggtatctt aaaaggctca gcaatgttta tgtcagattt aattaaacga
     4081 attgataccc atttatcaat tgatttcatg gatgtttcta gttatcacgg aggcactgag
     4141 tcaactggtg aagttcaaat cattaaagat ttaggttctt ctattgaaaa taaagacgta
     4201 ttaattattg aagatatctt agagactggt actacactta agtcaattac tgaattatta
     4261 caatctagaa aagttaattc attagaaata gttactttat tagataaacc aaaccgtcgt
     4321 aaagcggaca ttgaagctaa gtatgtaggt aaaaaaatac cagatgaatt tgttgttggt
     4381 tacggtttag attatcgtga attataccga aacttaccat atatcggtac gttaaaacct
     4441 gaagtgtatt caaattaatt ttttaatcaa tttcagttat tattactatg cgtttgagaa
     4501 ataatagtgt agactcaaaa atatgaaaaa tgtatttcat atatatttaa ttttagacaa
     4561 gacatatgtc ttgaaaagtt gaaaaatata gagattgata aaactaatac gggtgtgaat
     4621 gacattgatg ttaagctcaa ttactagctt ataaaacatg tcatatgtta caatttttgt
     4681 tagttttatt atgggaagta ggaggaaatg acgcatgcag aaagcttttc gcaatgtgct
     4741 agttatcgta ataataggcg ttattatttt tggtctattt tcatatttaa acggtaatgg
     4801 aaatatgccg aaacagctta catataatca atttactgag aagttggaaa aaggtgacct
     4861 taaaacttta gaaatccaac cacaacaaaa tgtctatatg gtaagtggta aaacgaaaaa
     4921 tgatgaagac tattcatcaa ctattttata taacaacgaa aaagaattac aaaaaattac
     4981 tgatgctgct aaaaagcaaa acggtgtaaa attaacgatt aaagaagaag aaaaacaaag
     5041 tgtctttgtg agtatacttt caacattaat tccagttgta gtcatagcgt tattatttat
     5101 tttcttccta agccaagcac aaggtggcgg tagtggcggt cgtatgatga actttggtaa
     5161 atctaaagca aaaatgtacg ataataataa acgtcgtgtt cgtttctctg atgtagcagg
     5221 ggcagatgaa gaaaaacaag aattaattga aattgttgat ttcttgaaag ataataaaaa
     5281 attcaaagaa atgggatcta ggattcctaa aggtgtctta cttgttggac ctccaggtac
     5341 tggtaaaaca ttacttgcta gagcggttgc aggtgaagct ggcgcaccat tcttctctat
     5401 tagtggttca gactttgtag agatgtttgt tggtgttggt gcgagccgtg ttcgtgactt
     5461 attcgataat gctaagaaaa acgcgccttg tatcatcttt atcgatgaga ttgatgctgt
     5521 tggtcgtcaa cgtggtgcag gtgttggtgg cggtcatgat gaacgtgaac aaaccctaaa
     5581 ccaattatta gttgaaatgg atggtttcgg tgaaaatgaa ggtatcatta tgatagctgc
     5641 tacaaaccgt cctgatatcc ttgacccagc cttattacgt ccaggtcgtt ttgatagaca
     5701 aattcaagtt ggtcgtccag atgtgaaagg ccgtgaagca attcttcatg ttcatgctaa
     5761 aaacaaacca cttgatgaaa cggttgattt aaaagcaatt tcacaacgta cacctggttt
     5821 ctcaggtgct gatttagaga acttattaaa tgaagcatct ttaattgctg tacgtgaagg
     5881 taaaaagaaa attgacatga gagatatcga agaggcaacg gatagagtta tagccggacc
     5941 tgctaagaaa tctcgagtta tttctaagaa agaacgtaat attgttgctc atcacgaagc
     6001 tggtcataca attatcggta tggtacttga tgaggcagaa gtagtgcata aagttactat
     6061 tgttccacgt ggacaagcag gtggttatgc aatgatgcta cctaaacaag atcgtttctt
     6121 aatgactgaa caagagttat tagataaaat ctgtggttta cttggtggac gtgtatcaga
     6181 agatattaac tttaacgaag tatcaacagg tgcttcaaat gacttcgaac gtgcaacaca
     6241 aatcgcacgc tcaatggtta cgcaatatgg tatgagtaaa aaattaggac cattacagtt
     6301 cggtcatagc aatggtcaag tattcttagg taaagatatg caaggtgagc ctaattattc
     6361 aagccaaatc gcatatgaaa ttgataaaga agttcaacga atcgttaaag aacaatacga
     6421 acgttgtaaa caaattttat tagagcacaa agaacaatta attttaattg ctgaaacatt
     6481 attaacagaa gaaacattag ttgctgaaca aattcaatca ttattctacg aaggtaaatt
     6541 acctgaaatt gattatgatg cagctaaagt tgttaaagat gaagattctg aatttaatga
     6601 tggtaaattc ggtaaatctt atgaagagat tcgtaaagag caattagaag atggacaacg
     6661 tgacgaaagt gaagatcgta aagaagaaaa agatattgct gaggataaaa aagaagctga
     6721 taaatctgat gaaaaagatg aaccagcaca tcaacaagcc ccaaatatcg aaaaacctta
     6781 cgatccaaat cacccagaca ataaataatc gattatattc agtacctctt tctatgataa
     6841 agttatagaa agaggtactt ttatcgtttt tgaaaatacg tattagattt taagtcgttg
     6901 aattgttata gcagaaaata attgtaaaac aagttacttc attatttaga atgatgggtg
     6961 tagaataagt acaattgttg cattttatga agtaaagtaa ttttttaaat atagagtaat
     7021 agaggagatt gaaataatga cacacgatta tattgttaaa gcattagcat ttgatggaga
     7081 gattagggct tatgctgctt tgacaactga aactgttcaa gaagcacaaa cgagacatta
     7141 tacatggccg acagcatctg ctgcaatggg aagaacaatg acagcaacag ctatgatggg
     7201 cgcaatgttg aaaggtgatc aaaaattaac tgtcactgta gatggccaag gacctattgg
     7261 acgaattatt gccgatgcaa atgctaaagg cgaggtgcgt gcttatgtag accatccaca
     7321 aactcatttt ccattaaatg agcaaggtaa acttgatgta agacgagcgg tagggacaaa
     7381 tggatctatt atggttgtta aagacgttgg aatgaaagac tatttctctg gagcaagtcc
     7441 aattgtttca ggagaacttg gtgaagattt tacttattat tatgctacaa gtgaacaaac
     7501 accttcatcg gtaggtcttg gtgtattggt aaatcctgat aatacgatta aagcagcagg
     7561 aggatttatc attcaagtta tgccaggtgc caaagatgaa acaatttcaa aattagaaaa
     7621 agcaattagt gaaatgacac cagtttctaa attaattgaa caaggattaa cgccagaagg
     7681 attactaaac gaaatcttag gtgaagacca tgtgcaaatt ttagagaaaa tgcctgttca
     7741 atttgaatgt aattgtagtc atgagaaatt tttaaatgct attaaaggat tgggcgaggc
     7801 tgagattcaa aatatgatta aagaagatca tggtgctgaa gcagtatgtc atttctgtgg
     7861 aaataaatat aaatatactg aagaagaatt aaacgtgttg ctagaaagtt tagcgtaatt
     7921 taatttaaat caatacgcta aaatgtttat ttttagcggt ttagtgaaat gtagaactaa
     7981 atagttgtat aatccttagt gattttgttt gctttctaga atttatttga taaaataatt
     8041 ctatatccga taaataaact aagatttcaa caactaacta aaaaggagtg ttcttaatgg
     8101 cacaaaaacc agtagataat attactcaaa ttattggcgg tacaccggta gtcaaattga
     8161 gaaatgtagt agatgacaat gcagcagatg tttatgtaaa attggaatat caaaatccag
     8221 gtggttctgt aaaggataga attgctttag caatgattga aaaagcagag cgagaaggca
     8281 aaattaaacc tggcgataca attgtagaac caacaagtgg taatacaggt atcggtttag
     8341 catttgtatg tgctgctaaa ggatataaag cagtatttac tatgcccgaa acaatgagcc
     8401 aagagcgtcg taatttatta aaagcatacg gtgcggaatt agttttaacg cctggatcag
     8461 aagcgatgaa aggtgcaatt aaaaaagcta aagaattgaa agaagaacat ggttacttcg
     8521 agccacaaca atttgaaaac cctgcgaacc ctgaagttca tgagttaact acaggtcctg
     8581 agttattaca acaatttgaa gggaaaacta tcgatgcgtt cctagctggt gttggtactg
     8641 gtggtacgtt atctggtgta ggtaaagttc tgaaaaaaga atatcctaac atcgaaattg
     8701 ttgctataga gcctgaggct tctccagtat tgagcggtgg tgagccaggt ccacataaat
     8761 tacaaggttt aggtgctgga tttattccag gcactttgaa tacagaaatc tatgacagta
     8821 ttattaaagt aggaaatgat acagcgatgg aaatgtctcg tcgagttgct aaagaggaag
     8881 gtattttagc aggtatttca tcaggtgctg cgatttatgc tgccattcaa aaagcaaaag
     8941 aattaggaaa aggtaaaaca gtagtaacag tattgccgag taatggtgaa cgctacttat
     9001 caacaccttt atattcattc gatgactaat taatgtcatt taaaagagtg agttatcttt
     9061 ttgagataac ttgctctttt tttctaccat gtatattttt aaaaatatga gcgttaaatt
     9121 aaacattttt ctgataaaaa tatccagtga atgataagat aataaacgta catactaata
     9181 actagtaaat agcaggagta aattttatta gagttaaaca atacataatt aaagggtggt
     9241 taacatgact aaaacaaaaa ttatgggcat attaaacgtc acacctgatt cattctcaga
     9301 tggtggaaaa tttaataatg ttgaatcagc tataaataga gtgaaagcca tgatagatga
     9361 aggtgctgac attatagatg ttggaggtgt ttcaacgaga cccggtcatg aaatggtttc
     9421 attagaagaa gagatgaaca gagtattacc tgttgttgaa gctattgtcg gttttgatgt
     9481 aaaaatttca gtcgatacat ttcgaagtga ggttgctgaa gcatgtttaa aattaggcgt
     9541 tgatatgatt aatgatcaat gggcgggtct gtatgatcat cgtatgttcc aaattgtagc
     9601 taaatatgac gcggaaatta ttttaatgca taatggaaat ggtaatcgtg atgaaccggt
     9661 tgtcgaagaa atgttaacat ctttgttagc acaagcacat caagctaaaa tagctggtat
     9721 accttcaaat aaaatttggc tagatccagg tataggtttc gctaaaacta gaaatgaaga
     9781 agccgaagtt atggcaagac tggatgaact tgttgcaaca gaatatccag ttttattagc
     9841 gacaagccgg aaacgtttca ctaaagagat gatgggttat gatacaacac cggttgaaag
     9901 agatgaagta actgcagcta cgactgcata tggtattatg aaaggcgtta gagcagtacg
     9961 cgttcataat gtcgagttga atgctaaatt agctaaaggt atagattttt taaaggagaa
    10021 tgaaaatgca agacacaatc tttcttaaag gtatgcgctt ttatggatat catggtgctt
    10081 tatcagctga aaatgaaata gggcaaattt tcaaagtgga tgtaactttg aaagtagact
    10141 tagctgaagc tgggcgtact gataatgtta ttgatacagt tcattatggt gaagtgttcg
    10201 aagaggttaa atcaattatg gaaggtaagg ccgttaattt acttgagcat ctagctgaac
    10261 gtattgcaaa tcgtataaat tcacaatata atcgtgtaat ggaaacgaaa gtgagaatca
    10321 ctaaagaaaa cccaccgatt ccgggtcatt atgatggagt aggtatcgaa atagtgaggg
    10381 agaataaatg attcaagcat acttaggatt aggtagtaat attggtgata gagaaagcca
    10441 gttaaacgat gctataaaga ttttgaatga atatgatggt attaacgtat ctaatatttc
    10501 tccgatttat gaaacagcac cagttgggta tactgagcaa cctaactttt taaatttgtg
    10561 tgttgaaatt caaacaacac tcacagtatt acaactgttg gaatgttgtt tgaagacaga
    10621 agaatgttta caccgtatta gaaaggaacg atggggtcct agaactttag atgtggatat
    10681 tttgttgtat ggagaagaaa tgatagattt accaaaactg tcggtgccac atccgagaat
    10741 gaatgaacgt gcatttgttt taatcccatt aaatgatata gcagcaaatg tcgtagaacc
    10801 acgttcgaaa ttgaaagtga aagatttagt ttttgtcgat gacagtgtaa agagatataa
    10861 ataatgtatt gttgagaaca ttcatattta ttgggaatag attattgatt attggagatg
    10921 tttccgcatt ttgattatct ctaaatgcat ttgatttcga attagtatat gatgatttag
    10981 tatgtgttag aattaagtta tttcataaaa tcaaagtaag caatatttga taaattgctg
    11041 taatttattt aggcttttgt tgaagtttgt ggaagataat ttttatacat ttacttaggg
    11101 ttgtattaat gataatcgta ttttaaatga ataagtaatt cataaacgaa aacagaaatt
    11161 gctttattac atttacaagc tcttgtaaaa ttaaaaaagt gataaaattg atgttatgaa
    11221 tataagaaaa catgccacat cataacttta aggtgtaatg gttaatgata aagtattaga
    11281 aacatcgaaa gagatgtatg ttgagcaaaa atgtctgata ttttataaaa ctttaaagga
    11341 aaatgtttga gtgtaccagt tggaatacta aaggattaca acaagttaaa ggagagaaag
    11401 ttatgtcaga agaaatgaat gaccaaatgt tggttcgacg tcaaaaatta caagaattat
    11461 atgatcttgg tatagacccg tttggttcta aatttgaccg ttcaggttta tctagtgatt
    11521 tgaaagaaga gtgggaccag tattctaaag aagaattggt agaaaaagaa gcggatagtc
    11581 atgtcgctat agctggacga ttaatgacta agcgtggtaa aggtaaagca ggatttgcac
    11641 acgttcagga cttagctgga caaattcaaa tttacgttcg taaagatcaa gttggcgatg
    11701 acgaatttga tttatggaaa aatgctgatt taggcgatat cgttggtgtt gaaggtgtaa
    11761 tgttcaaaac aaatactggc gaattatcgg ttaaagcgaa gaaattcacg ctactaacta
    11821 aatcattgcg accattaccg gataaattcc acggtttaca ggatattgaa cagagatatc
    11881 gtcaaagata tttagattta attacgaacg aagatagcac tcgtacattt attaatcgta
    11941 gtaaaatcat tcaagaaatg cgtaattatt taaataataa aggtttcttg gaagtagaaa
    12001 cacctatgat gcaccaaatt gctggtggag cagctgctag accatttgta acacatcata
    12061 atgcattaga tgcaacgtta tacatgcgta ttgctattga gttgcattta aaacgtttaa
    12121 ttgtcggtgg acttgaaaaa gtatatgaaa ttggtagagt attccgtaat gaaggtgtat
    12181 caactagaca taaccctgaa ttcacaatga ttgaattata tgaagcatat gcagattatc
    12241 atgacattat ggatttaaca gaatctatgg tgagacatat tgccaatgaa gtgttaggtt
    12301 ctgcaaaagt acaatacaat ggggaaacga tcgatttaga atctgcttgg actcgtttgc
    12361 atattgttga tgctgtaaaa gaagctactg gtgtagattt ttatgaagtt aaaagtgatg
    12421 aagaagctaa agctttagct aaagaacatg gtattgaaat taaagataca atgaaatatg
    12481 gtcatatttt aaatgaattc tttgagcaaa aagttgaaga aacacttatt cagccaacgt
    12541 ttatctatgg tcatccgact gaaatttcac ctttagcgaa gaaaaatcct gaagatccta
    12601 gatttactga tcgtttcgaa ttgttcattg taggtagaga gcatgcaaat gcatttactg
    12661 aattaaatga tcctattgat caaaaaggtc gttttgaagc gcaacttgtt gaaaaagcgc
    12721 aaggtaatga tgaagcgcat gaaatggatg aagattacat tgaagcgtta gaatatggta
    12781 tgcctccgac aggtggtctt ggtatcggta ttgacagatt ggttatgtta ttaactgact
    12841 ctccatcaat cagagacgta ttattattcc cttatatgag acaaaaataa atgacgttga
    12901 ttgttagtaa gagctctcgt gtatacaaca tgtgtatgcg agggctttct taattatggt
    12961 aattagttcg tgtttgaatg tttttgatag taatagttaa cgatagtggt gctatttttg
    13021 actgttaaac aaggtagttg gctgatagat gaaaatgacg atacgtatat agagtagttc
    13081 ggatgagaaa tgttaacgat agacattgag atatcttata acaaaaattg ctatattagt
    13141 ataatttatc ttaatcagct ataaaagtac tttaaaattg tatagaatgt gtatggtttt
    13201 gtacatatgt gtatgataga atactaaaag tgttatgaat tagagggcac gagaaatgtc
    13261 agttttgaag aataaaaaag ttgattaaaa gtgttgactt tatcaattga atgaagtaat
    13321 atataaaagt cgccaaaaac agacgaaaca cactaaaagc tgatgtgaca aagtttacat
    13381 caaagtgtaa aattaactat tgcaccttat taattaagcg tgtatcatga ataagtaagt
    13441 tatt
//
LOCUS       DANRDI010000066        13015 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.066, whole genome shotgun sequence.
ACCESSION   DANRDI010000066 DANRDI010000000
VERSION     DANRDI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.066"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(32..244)
                     /gene="atpE"
                     /locus_tag="PZ377_001470"
     CDS             complement(32..244)
                     /gene="atpE"
                     /locus_tag="PZ377_001470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDM6358344.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(287..1015)
                     /gene="atpB"
                     /locus_tag="PZ377_001471"
     CDS             complement(287..1015)
                     /gene="atpB"
                     /locus_tag="PZ377_001471"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDM6358345.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(1036..1488)
                     /locus_tag="PZ377_001472"
     CDS             complement(1036..1488)
                     /locus_tag="PZ377_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDM6358346.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(1556..2683)
                     /gene="wecB"
                     /locus_tag="PZ377_001473"
     CDS             complement(1556..2683)
                     /gene="wecB"
                     /locus_tag="PZ377_001473"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDM6358347.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(2707..3336)
                     /gene="upp"
                     /locus_tag="PZ377_001474"
     CDS             complement(2707..3336)
                     /gene="upp"
                     /locus_tag="PZ377_001474"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDM6358348.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(3364..4602)
                     /locus_tag="PZ377_001475"
     CDS             complement(3364..4602)
                     /locus_tag="PZ377_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDM6358349.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(4629..5153)
                     /locus_tag="PZ377_001476"
     CDS             complement(4629..5153)
                     /locus_tag="PZ377_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDM6358350.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(5260..5679)
                     /locus_tag="PZ377_001477"
     CDS             complement(5260..5679)
                     /locus_tag="PZ377_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDM6358351.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(5676..6722)
                     /locus_tag="PZ377_001478"
     CDS             complement(5676..6722)
                     /locus_tag="PZ377_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDM6358352.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(6806..7642)
                     /gene="prmC"
                     /locus_tag="PZ377_001479"
     CDS             complement(6806..7642)
                     /gene="prmC"
                     /locus_tag="PZ377_001479"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDM6358353.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(7629..8705)
                     /gene="prfA"
                     /locus_tag="PZ377_001480"
     CDS             complement(7629..8705)
                     /gene="prfA"
                     /locus_tag="PZ377_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDM6358354.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(8706..9305)
                     /locus_tag="PZ377_001481"
     CDS             complement(8706..9305)
                     /locus_tag="PZ377_001481"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDM6358355.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(9652..9906)
                     /locus_tag="PZ377_001482"
     CDS             complement(9652..9906)
                     /locus_tag="PZ377_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDM6358356.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(10024..11340)
                     /gene="rho"
                     /locus_tag="PZ377_001483"
     CDS             complement(10024..11340)
                     /gene="rho"
                     /locus_tag="PZ377_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDM6358357.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(11587..13014)
                     /locus_tag="PZ377_001484"
     CDS             complement(11587..13014)
                     /locus_tag="PZ377_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDM6358358.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
BASE COUNT         3891 a         2640 c         1908 g         4576 t
ORIGIN      
        1 gcgctttgtc ccgacctctt ttatctgtta attatccagc aaatgtcatg aatgcaatta
       61 ctacaccgat gataggtaat gcctcaacta aacctacacc aatgaacatg atacccatta
      121 attgaccacg tgcttctggt tgacgtgcta caccttcaac tgttcttgaa acgattaaac
      181 cgttaccgat acctgctcct aatgctgata aaccaattgc gattgctgct gcgattaaat
      241 tcataattta atttcctcct gtgattgtat ataattattg aaatttttag tgttcatctg
      301 ccactttatg tgacatataa accatcgaaa gcataataaa gatatatgct tggattgttc
      361 ctacaaatat tgaaaatgct tgccaaacaa ttaatcctgg gatactaata atccaacccc
      421 atgctggttc gttaaagaat aagccagcaa gtaatgttaa tagtatctca cctgcaaata
      481 tgttaccgta caaacgcaga ccaagcgtta atgttgaagt gaactcttca aaaacattaa
      541 taattgccaa tggccaaaac ggctgtacat aacctttaag atattgtttc gtaccacgca
      601 ttttaattcc ataaaagtga gttaacagta ttatcgttgt agacaacgtt aaagtcactg
      661 ttgcatcagc tgtcggtgat ttccaccaca atgtgtgatc tttcgttact atagaaaacg
      721 gaagacctaa catattagct ataaaaatgt acagaatcag cgttactgct aagaagtgga
      781 attgaccacc ttttttccaa gccatgttac cttcaatgat tcccctcacg aaatcaaaaa
      841 tccattcaac gaaattttgt ttgccagttg gtcttttttt caaattacgc gtacaaatga
      901 tagcaagtag aaaaacaaga aacgccgtaa caagtatcat caatatactt gataaattga
      961 aaacgatatc aaaaccgaat aaattccaac tcacgagcgg ggatttgtga tccataatct
     1021 cacctctttc ttaatttatt tgctcacctt tagtaaagga cgtatgataa ctacaacata
     1081 tgaaatcatt aaaccaataa ttataccgat gatacttaca tgcgctttat taaggtacca
     1141 aatcatacag gcaataattg caactaagta tcgccacata tttccagttg aaatatgcat
     1201 agtgtctggt cttttagctt tagctaaata gcattcaaaa atacatgtgt tgattgcaga
     1261 accagtaaca ccaatgatta atcctaagat aaatgcatgg tgtgtataaa ggtataatcc
     1321 tcccaatatt attattagat aaaaataata ttgaatgaac tgtttaaaaa tgttgtagaa
     1381 acgactcatt ttatatgcct ccgtgtgact gatatcaatc atgaaaaccg ttccactcat
     1441 acaaactaaa tgataatata ggcgatttgt ggtgtcaatt ctattcaaat tgttgtaaaa
     1501 aaataagcat agttagacac aatttagaca caattagaca aaaagtctat tttcctcaga
     1561 aatcgcttgg tttttcagtg attaaattca aataatactt aatatgatta actattcttt
     1621 ctgaagcaaa tccatcaccg taaggattag atgcttcaga catttgatga tataatctct
     1681 catcatcgat taattctttt gcagcttgat acacattttg cttatttgtt ccaatgactt
     1741 ttaatgtgcc agcttcaaca ccttcaggac gttctgtaac acttcgcaaa actaaaactg
     1801 gcttattaaa tgatggcgct tcttcctgaa ttccacctga atctgtcaaa ataaaataag
     1861 attttttagc aaaattatgg aaatctacta cgtccaaagg ttcaatcaat tcaattctgt
     1921 catgactacc taaaatcttt tgagccacct ctcgaacttt cgggttttta tgcattggat
     1981 ataccagtgc taaatcagta tactcatcta ttaagcgtct aaccgcttta aatatatttt
     2041 ccatgggttt cccgatattt tctcgtcggt gtgctgtcat aagaatgaat tttttgtcat
     2101 ggtatttatc catgatgtta gatttataat tgtcatcaac tgtatatttc atagcatcaa
     2161 tcgcagtatt accagtgaca acaacacttt ctgaatattt cccttcactt aacaaatgcg
     2221 atgcagcatt tttagtaggt gcaaaatgta agtcagctaa tacaccaact aattgtctat
     2281 tcacctcttc tggaaaaggt gaatatttat cataacttct aagccctgct tcaacgtgtc
     2341 caatcggcac ttggttataa aatgccgcta aaccacctgc aaatgtcgtc atcgtatcac
     2401 catgtacaag taccatgtct ggtttttcta attgaatcac ttgttctaat tgagtgattg
     2461 atttagaagt tatctcagaa agtgtctgtc ctgatttcat aatattcaaa tcgtattttg
     2521 gtttgatttc aaaggtactt aatactgaat caagcatttc tctatgctgt gctgtaacaa
     2581 caacaattgg ctcgagcatt ttttcttgtt ccaaagcttt aactaaagga gccatcttta
     2641 tggcttcggg tcttgttcca aatatggtca taatcttttt catcaaacta cttatctccg
     2701 attcttctat ttagtaccaa acaatctatc tccagcgtcg cctaaccctg gtgtgatata
     2761 tgctttgtca tttagctttt catcaagtgc agcaatataa atatctacat ctggatgtgc
     2821 ttcatgcatc ttttctacgc cttctggtgc tgcaattaaa cacatgaagc gaatattttt
     2881 agcgccacgt ttcttcaatg aagtaatagc ttcaattgct gatgcgcctg ttgctaacat
     2941 aggatcaaca acaatgattt gtctttcagt aatatcttga ggtaacttag caaaatactc
     3001 tacagccttt aatgtttcgg gatctcgata taaaccgata tgtccaactc tggctgcagg
     3061 tactaaactt aaaataccat cagtcatacc taaaccagct cttaaaattg gaacgatagc
     3121 taatttttta ccagctaatc gtttagccgt cattttagtt acaggcgttt caatatcaac
     3181 atcctgaagc tctaagtctc tagttacttc atatgccatc aacataccaa cttcgtctac
     3241 aagttctcta aattctttag tacctgtatt tacatctcta atatagctta gtttgtgttg
     3301 aattaatgga tgatcgaaaa cgtgtacttt actcataaaa attactccta tctttgtgta
     3361 tgtttattga tatagaggat attcagctgt taatttcgca acgcgttctt tagcttgttg
     3421 taatttttct tcatctttac tatttttcaa tgctaaactg atgatttttg caacttcctc
     3481 aaaagctttt tcatcaaatc cacgcgttgt tgcagcaggt gtacctaaac gtataccact
     3541 cgttacaaaa ggtttttctt gatcgaacgg aatggtattt ttgttacatg tgataccaac
     3601 tgaatctaaa gtctcttcag cttctttacc agtaagtcct atagaccctt ttacatcaac
     3661 agctactaag tgattatctg taccgccaga aacaattcta aatccttcat taattaatgc
     3721 ttctgcaaga acttttgcgt ttttaaccac ttgttgttga tacgttttga aattattttc
     3781 taacgcttct ccaaaagcaa ctgcttttgc tgcaataaca tgctcaagag gtccaccttg
     3841 aataccaggg aaaattgttt tatctatgtc ttttttatat tcttccttac ataaaatcat
     3901 accaccacgt ggtccgcgta atgttttgtg tgttgtagtt gttacaaaat cagcatattc
     3961 tactggattt ggatgtaaac ctgccgctac taatcctgca atatgtgcca tgtctaccat
     4021 taacttagcg tttacttcat ctgcgatttc tttaaacttt ttgaagtcaa ttgttcttga
     4081 atatgctgat gctcctgcca caataagctt aggcttatgc tctaacgcta atttacgaac
     4141 ttcatcataa ttgattcgtt ctgtgtcttt atctactcca tattcaacga aattgtagaa
     4201 tttaccacta aaattaacag gcgctccatg tgtcaagtga ccaccatgac tcaaattcat
     4261 acctaaaact gtgtcgccca ttcctaatgc aactaagtaa acagccatgt tcgcttgtga
     4321 acctgaatgt ggttgaacat tgacatgttc agctccaaac aatgctttag cacgatcaat
     4381 tgcgatgctt tcagtaacat ctacaaactc acagccacca taatatcggc gtcctggata
     4441 gccttcagca tacttattag tcaacactga accttgtgct tccataaccg cttccgatac
     4501 aaaattttcc gatgcgatta actctatgtt gctattttgt ctctgaaatt ctctctcgat
     4561 tgcttctgcg ataactttat cttgcttggt gatataagac ataaaatctc cccttctttc
     4621 aaaaaaactt attggtattt agcacgttcg ccaccaatct ttttcggcct agatgtggca
     4681 atagttacaa ttgcctgtcc tacttgcttt actgaggtcc ttacaggtac acatacatgc
     4741 ttaatatgca tgcctattaa cgtttgacca atatcaattc cacaaggaac agtaatatgt
     4801 tcgaccacga tcggatcctt catatgctga aaagcgtatg ttgccaaact ccctccagca
     4861 tgtacatctg gaacgacgga aacttcttcc attgttaatg gattatactg agatttttct
     4921 attgttatcg ctctgttgat atgttcacat ccttgaaaag caaaagtaac gcctgtctct
     4981 ttactcacaa catctaatgc attaaaaata gtttctgcaa cttccatcga accgacagtc
     5041 cctatttttt cgccaatgac ttccgatgtt gaacatccaa ttaaacatat atctccttta
     5101 ttaaaaaagg acatatcttt taattcgtct aataacattg tcaaatcttt cataaaagcc
     5161 cacccttcct aaaaataaaa aaggaatata gcaaagtgct acactcctct attataactt
     5221 atttaactgt taacatatac taattataca gaattcctac tagcaaataa tatcttttaa
     5281 ttttaaaatt aaacttacaa gttcttcata ggtatgtaca tacatttctt ttgttccacc
     5341 gtatggatct ataacttctc ctgcttcttt tacatattca tgcaatgtga aaacatgatt
     5401 ttgcaaacca aagtgtgcct ctattaattc tttgtgcgaa tacgacatcg tcaaaataat
     5461 atctgctttc aaatctgctt cagtaaattg ttgcgataag gtcgtttcag ctaaatgatg
     5521 ttcttcaact aagtcttcaa cataattcga aacaccttga ttgttcacag cgaatatacc
     5581 tcttgattca aattgatgat ttggcataac ctcttttgca atactttccg ctaatgggct
     5641 acgacatgtg ttacctgtac aaacgaataa aatcttcata gttcacatcc tttaataatg
     5701 tgattacctg cagcttttaa catgcgattc ataattgctt ctgtattatc attcagctca
     5761 aagccgtata tatacgccgc tgaaatattt tcattttcat caagtgaatg taacacatca
     5821 taaagattat gacttgcttg tttaacatca ttgtcatcct gacataattg aatgaattgc
     5881 gcttcacttg gtataaacgc caccttatta ctcggcacaa taaaagctat agaagaccaa
     5941 tctttaccgt catttccaat tttgctctca atatctgtaa taattgtaag tggtgtattg
     6001 ggtgagtaat gcttatactt catacctggt gcaattggct gttcagtatc attataatca
     6061 gcatgggcga tactattcgg aagtatttct gtaatcattg ctgctgttat agaaccaggt
     6121 cttgcaattt tataaggaaa agatgtgcaa tctaaaaccg tactttctaa tccttcttca
     6181 ctttgttcag cttgaacaat accatcgata cggccattca aatcttgata tacatgattg
     6241 aaagttgttg gtgaaggtct accacttaaa ttagcacttg gagcagctag aggttcattt
     6301 atgatttgta ataattgtct acctacagaa tggcttggca ttctaacagc aactgatgat
     6361 aaacctccag aaacttttcg acatagatag cctagcttta acggcaatat aaacgaaata
     6421 gggcccggcc agaatgcctg cattaacttt tctacgcgtg gatccaaagt atatgtaaaa
     6481 tcttttaatt gacctttact gtgtatatga acaataagcg gattgtcaga tggacggcct
     6541 ttagcttcat atattttagc tacagcttct tcatctgtcg catttgctgc aagtccataa
     6601 actgtttcag ttggtaaacc tattaaacca ccgtttaaaa caatgtcttt tatttcatta
     6661 attttaggat attgctgtaa atcttcatta tattctctaa catcccaaat tttagtatcc
     6721 aacttaatca cgcctttctt atttatcata atataaagca aaaagctatg cacttaacta
     6781 atcatagcaa aggcataact tctaattacc atttaaatga gacgattcga tcgtggccat
     6841 ttatatcttt aataatgtcg atttttttgt caggaaattt atttaaaatt attgatttaa
     6901 gtgcctcacc ttgattgtaa ccaatttcaa aaacaactgg gctgcctttt tccataacgt
     6961 gaggtaaatc ttcaatgatt gattcataaa tagcatatcc atggttatct gcaaacaatg
     7021 cctgatgtgg ttcgaatctc gtaaccgttg gagacatcgt aaccatatct ttttcatcta
     7081 tatatggtgg attagatatc aagccgttca acttgatacc ttcattaatt aagggcttta
     7141 atgcatcccc tgttaaaaat tgtatttgtg attgatgctt ctcagcatta ttacgagcca
     7201 tattcattgc ttcaagtgaa atatcagtag caataacatt taaatccggc ttttcacatt
     7261 tcaaagtaat tgcaagtaca ccactacccg ttccgatatc tacgattgtt gcatcatctt
     7321 ctaactgttg taagaaatgc aacattactt cttcagtttc aggtcttggt atcaaacaat
     7381 ttgagtttac atcaaacgtt ctaccataaa atgaggcaaa gccaactata tactgtatag
     7441 gctctcctaa taacatacgt tgtaatgcta agtcgaactt cataatcatc gctttcggca
     7501 tatcatcatg catgtggact acaaagtccg tacgcgtcca ttgaaataca tctaacatta
     7561 accattcagc tcgtgtttgt tcaaaccctt tttgttgtgt taaatgaatt gcttcatcta
     7621 acttttcttt ataattcacc attattaagt tctttcaatt tatctgtctg ctctgataaa
     7681 gtcagtgcat ctataatttc ttctaaatgg ccttccataa tttgccctaa tttttgaagc
     7741 gttagaccta tacgatggtc tgttacacgg ctttgtggat aattataagt tcgaatacgt
     7801 tctgaacgat caccagtacc gactgctgat ttacgttgtg acgcatactt ttgttgttct
     7861 tcttgaactt tcatatcgta taaacgtgct tttaacactt tcattgcttt ttcacggttt
     7921 tgaatttgag acttctcaga agatgttgca atgacaccag ttggtaaatg ggtaatacgt
     7981 actgcagagt cagttgtgtt tacgtgctga ccacctgcac cacttgaacg atacgtgtcg
     8041 atttttaaat cttcatttct aatttcaatt tctacatctt caacttctgg taaaactgcc
     8101 actgtagctg ttgaagtatg aatacgtcca cctgattctg tttcaggcac acgttgaacg
     8161 cggtgcgcac cattttcaaa tttcaattta ctatacgcgc cattaccaga aactgagaaa
     8221 ctaatttctt tgtaaccacc atggtcactt tcagacgctt ctactatttc agttttgaat
     8281 ccttgtgatt cagcatactt tgaatacata cgcattaaat caccagcaaa aatcgcagcc
     8341 tcatcaccac ctgctgctgc tcttatttct acaataacgt ctttgtcatc attaggatct
     8401 ttaggaatca ataatatttt aagctcttct tcaagatttg gaagttcagc tttaatacca
     8461 ttactctcct cttttaacat ttctacttct tctttatcat cagtctcact taacatttct
     8521 tcaatatcag ctaattcttc ttttttagct ttatagttac gataaacatc tacagttttt
     8581 tgtaaatcag cttgctcttt agaatattta cgtaatttat ctgaatcatt tacaacatct
     8641 gggtcactta acagttcatt taactgttcg tatctttctt ctacaatatc taattgatca
     8701 aacacttata attcctcctt attattatca ctaggtgcta cgatatggtg cgcgcgacaa
     8761 cgtggctcat aactttcatt ggcacctact aagataatcg gatcatcgat tttagctggt
     8821 ttaccattta ttaatcgttg cgttctacta gatgaagaac cacaaacagc acaaactgct
     8881 tgaagtttcg ttacttgttc actgacagcc atcaatttag gcattggttc gaacggttcg
     8941 cccctaaaat ccatatctaa tccagcaaca ataacacggt gtccatctgc tgatagtttt
     9001 tctactatac ttacaatttc atcgtcaaaa aattgcactt cgtctattcc tataacatca
     9061 acattagtta agtcgtgcgt cataatttca cttgctttag aaatattaat cgcttcaatg
     9121 gcattaccat tatgagagac cactttttct ttatgatatc gatcatcaat cgccggttta
     9181 aatacaacga ctttttgttt agcgtatata ccccttctta gacgtcttat tagttcttcg
     9241 gatttaccgc taaacatact acctgtaata cattctatcc aaccggaatg gtaagtttca
     9301 tacattgaga gttccacctt tttcaaaaca taatcgcttt attatatcat atttcaaata
     9361 ttcataaatg tctttttcat aattatatcg atattgtaca tgaacaatta ttttataaat
     9421 ttgtcttgca tatttatata cagctattct aatttgttgc ttcttattcg tgcgatgttt
     9481 ctactcaata caaactttaa taaaggggga aatttatcat tatgtattaa caacatggta
     9541 ttgagtcact tctttgaaca cagtcgtaag tatataccct tactacgctt ataaataaaa
     9601 aagctgtcta caattccatt ataggatgca gacagcttaa cgattactat attagttgtt
     9661 tgatttaaga ccaaactttt tgttgaaacg ttccacacgt ccatccgcag cagcgaattt
     9721 ttgacgtcca gtgtagaatg gatgtgaatc agatgaaata tctaaacgaa taactgggta
     9781 ttcttttcca tcttcccact ccatcatttc tgaagatgtt tttgtagaac cgcttaagaa
     9841 tttgaagttt gtagtagtat ctaaaaagat tacttggtga tactcaggat gaattccttg
     9901 tttcattatt ttcagctcct ttgccctgaa ccatctggaa cagagttatt tgtgagtttt
     9961 tacccaatac tgtgtaatta taaaggtaat taattcaaaa cgcaagcccc tatataatgt
    10021 ttattaaatt ataggtcgac ccgttttagt actttcttct gcagactttt gtagctgctt
    10081 gaagaaatct tcattattct tagacctttt aagtttgcga ataaatcttt cagtaaagtc
    10141 agttgagtca gtgaatagat ttcttaattg ccataatgtg tctaattcag atttacttat
    10201 caacaattct tctttacgcg ttgaacttct gccaatatca attgcaggga agatacgacg
    10261 ttcagacaat ttacgatcta aatgtaactc catgttacct gttcctttaa attcttcgta
    10321 aatcatatcg tccatacgtg aacccgtatc aactaatgca gttgcaagta ttgttaaact
    10381 tccacccgct tcaatatttc tcgctgcacc gaagaatgct tttggtttgt gtaaagatgc
    10441 aggatctaaa ccacctgata atgtacgacc acttggtgga ataactaagt tataagcgcg
    10501 tgctaatctc gttatagaat ccattaaaat aatgacatct tccccaattt ctactaaacg
    10561 ctttgcacgt tcaagtaata attcagctac tttaacatgg tgttctggtg gttcgtcaaa
    10621 cgttgaatga acgacttcag cagcttctac tgagcgttct aaatctgtta cctcttcagg
    10681 acgctcgcca actaacaaaa taaatagctt tgcatctggt ttgttcgtac tgattgcatt
    10741 cgctatttct tttaataacg atgttttacc tgctttaggt ggcgccacta ttaaaccacg
    10801 ttgacctaaa ccaatcggtg ttactaaatc catgatgcgc gttgaataat tttgtgtttc
    10861 tgtctctaat ttaatacgct catctggata aagtggtgtc aaagcttgga aatgcggacg
    10921 tttcttcact tcttctgcgt tatggtcatt gacaaagtca acttgtaata agccataata
    10981 tttttcgtta tctttaggtt ttctaacttt cccagttact ttatccccac gtttaatttc
    11041 aaaacgacga atttggctag cagatatata aatatctttt tcccctttag aatagttcac
    11101 tgttcttaaa aaaccataac catctggttg tatatcatct aagatacctt ccatataata
    11161 gttaccatct ttttccattt gtgcttccat aatagctaga acaagttctt ttttatttaa
    11221 tttactatag ttcgtcaact taagagtttt agctttttga gtgagttcct tggtagtata
    11281 gttcttgtac aattcgtgga atgattcata ctgaggagat gtacgttctc tttcaggcat
    11341 atagtttaca cccatttcat tattttttag ttagtaagta atagataggc atgacaatgc
    11401 ataaataact gtgctagcac aatcccatta tgctaatccc caacctttta aatacatgaa
    11461 taactatagc aaatttttat tttaattaca aaatctattt ataaaaattg caaaattaaa
    11521 acagtacaca aaacttaatt agtcatgtgc actgttttat tgcgattttt aattcaaaaa
    11581 tcaaatttat ttaaaatatc cagctataga tttcacttct aagaactctt caataccata
    11641 atcgccccat tcacgaccta aaccagattg tttatagcca ccaaatggta aatctggctt
    11701 tctacctgct tcgtttattt ctactgtacc tgcttcaata gaacgagcta ctttatgcaa
    11761 tgtttctttg tccttaccaa taacatatcc tgccaaacca tattttgtat catttgcaat
    11821 ttgaatcgct tcatctaaat cgttataagt gataactgac attactggcc caaaaatttc
    11881 ttcttgtgct atcgtcattt gattatctac attaataaaa attgtcggac gtgcaaagta
    11941 tcctttttca agtccttctg gtttaccagg accaccataa aataattcag caccttcttc
    12001 aataccttta ttaatataat tttgtacttg atcaaattgt tttttactaa tgatagggcc
    12061 tacttgtgta ccatcttctc ttggattacc gacacgcact tggctaaatt gttcttttaa
    12121 ttcagctaag aatgcatctt taattttgtt aggcactaaa acacgtgtac cagctgtaca
    12181 tacttgacca gtattattaa caactttgcc tgttgttgct ttagccgctt ctttaatatc
    12241 tacgtcatct aggacgatat atggtgattt gccaccaagc tctaatgata cctttttaaa
    12301 atctttagcg gctttttcca taattttaga accagtaggg cctgatcctg taaatgacat
    12361 catgcgtact ttaggatgtt cagataaagg attcccaaca ccagcaccat caccattaac
    12421 aaggttaaat acacctttag gaacaccgac tttatcaaaa atctcagcta aaataacagc
    12481 tgcaaatggt gtttcttcag atggtttaag tacaactgga ctaccagccg caaatgctgc
    12541 tgctaatttt aatgatgttt ggtttgtagg gaagttccac ggtgtaatta atccagatac
    12601 accgattgct tctttaacaa ctaaatcatc tccgcggcgt tcttcaaatt cgtagttatc
    12661 taatgcgtct ctcgctgcaa caaaatggtt tagtcccatt tgataatgga cacgctcaga
    12721 taatgataaa ggagcaccta attcatccgt aatagcttgt acaatatcgt cttttctgtt
    12781 ttcatattct tttacaattt tatctaataa cgcttgtctt tctttcacag atgtatgacg
    12841 gaactctaaa taaacatcgt ctgccgcctc gacggcttta tcaacatcag ctttattacc
    12901 tttagcaact ttcccgatta cttcttcggt tgctggattt ataacttcta tcgtttcatt
    12961 actattactt tctacccatt cgccattaat gtattgcttt gtgtagtctc tcatt
//
LOCUS       DANRDI010000067        12999 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.067, whole genome shotgun sequence.
ACCESSION   DANRDI010000067 DANRDI010000000
VERSION     DANRDI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.067"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(28..1086)
                     /gene="hisC"
                     /locus_tag="PZ377_001485"
     CDS             complement(28..1086)
                     /gene="hisC"
                     /locus_tag="PZ377_001485"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDM6358359.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(1327..2841)
                     /locus_tag="PZ377_001486"
     CDS             complement(1327..2841)
                     /locus_tag="PZ377_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDM6358360.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(2834..3811)
                     /locus_tag="PZ377_001487"
     CDS             complement(2834..3811)
                     /locus_tag="PZ377_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358361.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(4033..5814)
                     /gene="recQ"
                     /locus_tag="PZ377_001488"
     CDS             complement(4033..5814)
                     /gene="recQ"
                     /locus_tag="PZ377_001488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDM6358362.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(5826..7709)
                     /locus_tag="PZ377_001489"
     CDS             complement(5826..7709)
                     /locus_tag="PZ377_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDM6358363.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(7980..9920)
                     /gene="ltaS"
                     /locus_tag="PZ377_001490"
     CDS             complement(7980..9920)
                     /gene="ltaS"
                     /locus_tag="PZ377_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDM6358364.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            complement(10394..11398)
                     /locus_tag="PZ377_001491"
     CDS             complement(10394..11398)
                     /locus_tag="PZ377_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDM6358365.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(11382..12092)
                     /locus_tag="PZ377_001492"
     CDS             complement(11382..12092)
                     /locus_tag="PZ377_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDM6358366.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(12180..12386)
                     /locus_tag="PZ377_001493"
     CDS             complement(12180..12386)
                     /locus_tag="PZ377_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358367.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(12452..>12999)
                     /locus_tag="PZ377_001494"
     CDS             complement(12452..>12999)
                     /locus_tag="PZ377_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDM6358368.1"
                     /translation="NRIKCSSERLNFKFDEHAWRNELNDVTTKYHSGQYRLKIVLNAE
                     SKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTTERKHLAHNHETDLILLTSEDG
                     KVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLIDSDKLVEKDFNKNELIYKYHN
                     NEIRLFLINSLREVADVHLCL"
BASE COUNT         4016 a         2348 c         1765 g         4870 t
ORIGIN      
        1 tatcatttct gtcccactcc ccacttacta ttcgtattta aagtttgata aaacttctaa
       61 cattttatca ttttgttctt taaaaccaat tgtaattcta acaccagttg gaaatggtcg
      121 cgtaatacac cctacattta aaagtgcttc ataaagttca tttacccgct ttgtttttac
      181 aaatataaaa ttcgtttgac ttggcaagaa atactcactt tgaggtaatt gataaaatct
      241 ttcgcgttca acactatttt tatgtgttac ctcttttaaa tattgttgat cttcaagtgc
      301 tgcaactgct gcgtattcag atatacgtgt cacattaaat ggtggtctaa tgatgttcca
      361 tttttcaatc gcatgttcac ttgctaccac atatcctaca cgtaaacccg ctaatccata
      421 cgcctttgaa aatgtacgta ataagaaagc attgtcatat ttttgttgca aagcaagtgt
      481 atccgggtag tcctctgctg tcacaaattc aaaataagct tcatctataa ttactggtac
      541 atgtggaggt acttgagata aaaacgaatc taagctctca tgattaaaat atgtacctgt
      601 aggattattt ggattacata accataccaa tgacgtatct tcattaactt cttttaaaat
      661 accttctaaa tcgaagccac catcttttaa aggtacttgt atcacattag ctgattcaac
      721 aatcgcattg tgataatatt gaccgaatgt cgcttcactt gtaacaatag tatcccctgg
      781 cgttaataca gctctagaaa tcattaatat aacttcatct aatcccgcac caaataaaat
      841 gcgtgattga tctacattta aatgtttact aatcgccgct tttaatgtcg gtgatcctgt
      901 ttcaggataa taatataact catctaagtg tgctgatatc gcttctttaa ctttaggcga
      961 tggtccatac aaattttcat ttgatgcaag tttatataaa tctccttcaa tgccatactt
     1021 ttctttcaat gcccttggag ataaaccagg ctgatatgct gatagttgat taagttgttc
     1081 tttcataata aaaacaacct ccttagaatt aactgaaatt tcaaaaaaac ttatattcat
     1141 tatatcgctc tatgtttcta aaatgcaatg ctactatttt cacatcataa tagcttacat
     1201 tgtgtttaac atgtcatatt ttttaatcat tattgcatgt ttaaattgaa cataattata
     1261 gatttaaata taacaacgcc acataagata taatttggtg ttacgcatct tatgtggcgt
     1321 tggtcgttat ttgattaacc cttttgcttt taaataatct ttcgcaactg tatatgggtc
     1381 ttcattttta actgttactt tatagttcat catttgcata tcttcatcag atattttgtt
     1441 ttctagtttg tttaacggtt tcttaatttc tggatgtttc tttaaaaagc tttctttaaa
     1501 taatggtgct ccttgatatg gtggaaatac gtgcttatca tcttttaaca caaccatatc
     1561 atattgtttt aattcagcgt cagttgaata tgcatctatt aaattaatat tacctttatt
     1621 gatcgcttga tatctcaact taggttccat tgtacgtatg ttatctaaat ttaaattata
     1681 agccttttga acagctttgt aaccatctgg acgatcatta aattccaatg taaatcctgg
     1741 tttaagttga tctttaacct tatttaaatc accaattgta cgtatattat gttgtttagc
     1801 aaaatcacgt tttacagcta aagcatacgt attgttatac ttcattggtt ttaacatagt
     1861 catttgatat ttcttttcaa gactttgctt agcttgttca taaacttttt tctcttcttt
     1921 tgacttcaat ggttcttttg ttaattcacc taaaactgtt ccagtaaatt ctaaataccc
     1981 atctatatcg tcagatttta aagcattaaa taaaaatgct gttttgccca taccatcttt
     2041 cacttctaca gtatttttgg tctcttcttc tattaaaatt ttatacatat ttgtaataat
     2101 cgatggctcg gagccaagct ttccagctaa cgtaatttta tcaccttttt gtgcaaacat
     2161 aggaatagcg atagccagta taataatcat cactataaca cctaacgtca tcaataactt
     2221 tttataagat aatttagcca taaatcttaa aattaaatca aatataattg ccaataaggc
     2281 tgctggaatt gcacctaata atatcaacga tgcattgtta cggtctatac ctaataaaat
     2341 taaatctcct agtccgcctg caccaattaa tgctgctagt gttgctgtac ctataattaa
     2401 taccatagcc gttcttacac cagccattat aacaggcatt gctatcggaa gttcgacttt
     2461 agttaaacgt ctaaatggtt tcatacctat acctttagcc gcttcaatga gtgatggatc
     2521 aacttcttta attccagtat acgtattcct taaaattggt aacaacgcat acactacaag
     2581 tgcaataatt gctggcacac gaccgatacc aaataaagga atcattaaac ctaataatgc
     2641 caacgatggt atggtttgaa gaattgccgc aatattcatt acgatttcag atatcgtttt
     2701 agtcttcgtt aataaaatac ctaatggtac cgcaatagca gttgcaatca ataatgcgat
     2761 aaatgatatt tgaatatgtt ctatcattgt cgaaaagagt tgccccttac gttcactcaa
     2821 tatgtcgaaa aagttagtca tgttgagcta cctccttttt ctgggacaaa tatttgaaga
     2881 tatctttcct atcaataaca tattgaccta cgctatcttc ttgcatgaca atgacacgct
     2941 cgctctctga taaaagttga tacaatactt caattggttg attgtcataa acaattggat
     3001 aagcgctcat agatgtaacc tcatcgattg gtttcataat atccaagtca cggataattg
     3061 cgttctcttc aacacatggc gcatcatctt ctaaatgact acccataaat tgtttaacaa
     3121 attcactttg aggattattt ttaaatcctt ctggtgtgtc aatttgttca atatgccctt
     3181 cattcaaaag acaaatctta tcaccaagtt tcatcgcctc ttgaatatca tgtgtaacaa
     3241 atatgattgt cttcttaatt ttagtttgta attcaattaa atcatcttga agtttttctc
     3301 ggctgattgg gtctaatgca ctaaacggtt catccattaa aataactggt ggatcagctg
     3361 ctaacgcacg tataactcct acacgttgtc gttgcccccc tgacaattca tcaggttttc
     3421 tgtttttata tttttcaggt tctaatccaa ccatttcaag taattcatct actcttttat
     3481 ctatatcttt ttctttccac tttttcattt gtggcacttg tgcaatattt tctttgattg
     3541 tcatatgtgg gaataatgca atctgctgca atacgtatcc aatatcccaa cgcatttcgt
     3601 atactggata atcacttatt ggtttatctt taaaataaat ataaccttca cttaagtgaa
     3661 tgagtcgatt aatcattttt aatgtcgtag tttttccaca acctgaaggt ccaattagca
     3721 caaaaaattc accctcatta atattgaaac taatgttatc gacagcaaca tgtttgccat
     3781 aacgcttagt tacattttta aacttaatca ctttgccacc tctttttttc tcatagcata
     3841 aaaccgagat tatatgtatg tattccctat ttaaccacgt ttattacaat tttcaaattt
     3901 aaatgattta tccttgaact tttttaataa aataatgaat aataggtaat ctccagttaa
     3961 gaaatagtgt tattttacct tgaattcaaa aaaacaccca gtaaaacaag gaatgcttac
     4021 taggtgtctt cactatactt tggctttata attttgaatc gtttctaaaa atgctggaca
     4081 ataatgtttt aatttgtaac tacctacgcc atcaatatta atcatatctt gtttcgaagc
     4141 aggcttacgt ttagcaaatt cctccaacgt gtaatcagaa aatatactta caggtgctat
     4201 cgttaatttg tcacttaact ttttacgaac ttctaccaac tgactgaata atactcggtc
     4261 aaccccttca accgtattta taaatacttt ttcagtcgct ttttgcttaa atggtgttgt
     4321 gaatacttct acttcattac tgagtaattt tttaattgaa gtatcacaca ttaatatttc
     4381 gtcattttca tttaagaacc ctttgaatct taattcatct attaagtgac ttaattctga
     4441 tgttgtgtaa cctttcatta aaccatgggt tgaaatttgg tcataacctt tatacttaat
     4501 ataatctgtt gactctcctc ttaacacttg aatgataaca ctataactct cttgttgttt
     4561 catacgagcg atgcaactaa taatcatctt agcttcttgt gtcatattat atgatttatc
     4621 ttgttgaaca caattactac attgttcaca ttcttctaat ttttcattcg gttcaaaata
     4681 atggacaatt gttgcttcta gacatttttt tgtttttgta tattgaatca ttttagttaa
     4741 cttttcgccc attttatctt tatagtcatc atcagcttga gagactgtta taaaatactc
     4801 gtgtaaattg atatcgcgtt cgctaaataa caaaatacat tcacttttca acccgtcacg
     4861 acctgcacga cccgcttctt gataataaga ttctaaatct ccaggcatat tataatgaat
     4921 aacaaagcgt acattggatt tatcaatacc cataccaaaa gcatttgtag caacgactac
     4981 tttaacacga tcaaataaga aatcattctg cgcttcttct ctttctttat tgctcaaacc
     5041 tgcatgatat ataacacttt caattttctg actttctaag gcttcttgaa gctcttcaac
     5101 ttgcttacgt gtagaacaat aaataatacc tgcatcttca tcgtgtgttt taatataatc
     5161 caatataaat ttttgacgtt gataagtagg atttacttta aaaattaagt ttctacgctt
     5221 agtactcgtt ttaatttgat cagtttgagc gatatttaac ttttctctaa tatcttgctg
     5281 tacttcaacc gtggcagttg ctgtcaacgc tattattgta aaatcttgag gtaacgtaaa
     5341 tacttttgaa ataacatttt ggtaactcgg cctgaaatca tgaccccatt tagaaataca
     5401 atgcgcttca tcaaacgcga ctaagtgaat ctttatacgc tgaagcatat ttaaaaaata
     5461 tcggttttca aatcgttctg gtgcaacata caaaaattga atttctccat ttgataatgc
     5521 tttttcaata cgttgttgct ctttttgagt caaactacta tttaaaaaag cagcttgaat
     5581 tcccatcgct tttaattgat ccacttgatc tttcattaat gatattagtg gacttattac
     5641 aattgttgta ccacctaaca ataaacctgg tacttgatag catatagact tacctccacc
     5701 agttggtaag acaccaagca cattacgatg gtctaatact ttgctaataa tttcttcttg
     5761 tcctggtcga aacgtttcat acccaaagta atgcgataat gtttgttgca tcataaattg
     5821 accccttatt gttgttcttt aatttcttct aactcactcc atcttgtgat gtctaaatca
     5881 tattgaattt caagttgttc tttttcttcg tttaattctt taattttccc ataatctgca
     5941 cttgcctcaa tcatgagcac atcaatttct tccattctta cttccgcttg ttctatgcgt
     6001 ttcatcaatt gttcatattc taatttttct ttatatgata aaccattttt cttacgtaca
     6061 gttgtagaag atttagattg ttgcttcaat gtggatttat ttttatctaa tgatttttta
     6121 taactttcat aatcttcaaa agttccgata atcttttcca tctgaccatc atgaataaac
     6181 caatatgact gtgcaacttt atttaagaag tagcgatcat ggcttacggt aatcactgta
     6241 ccaccgaaag tatgaatata atcttcaagt attgttaaag tctcagtatc taaatcattt
     6301 gtcggttcat ctaacaacag aacatttggc tggtgtacga gtagacgtaa taaatacaaa
     6361 cgcttttgct ctccaccaga taatttataa acttttttac catgagttgc acttggaaat
     6421 aaaaatcgtt caagaagttg tgtaatagat accgaggttc catctttttc ttttgcgatc
     6481 tcattttctt ctcttaaata atcaatcata cgaatatctc tatccagggt ctcatctgtt
     6541 tgcttaaaat aagctacttt aaccgtctgc ccagtcttca atttaccttc gaattgttgg
     6601 tcttctccac tcaaaatatt taagagtgtt gttttaccag ctccatttgg cccaacaaca
     6661 ccaatacgct cacctttttg aataatttcc gtcagatgtt caattaatac tttatcatta
     6721 atagcctttg ataagtcttc taattcgaac acttgcttac ctaatcttga ataagcaaga
     6781 ttcaattcac ctttatcgtc tttatattgc tggttaactt cattttctag gtcattaaat
     6841 cgattaattc tagcttgttg ctttgtagta cgagccttag ctccagccct catccaagca
     6901 agttcttcct tatataaagc tcgttgcttt tgttgttgct tttgaagtgt tacttctctt
     6961 tcagcgcgca tttcaatata agattcatag ttaccaggat atgacgctaa cttacctctg
     7021 tttagttcaa taattctagt ggaaacttca tttaaaaaat atcgatcatg ggttacgaat
     7081 aaaacagtat gaggatattg cttcacataa ttgatcaacc agctgattga ttcgaagtct
     7141 aaatggttcg taggttcatc taataacaat aaatctggtt gttctattaa tgttttagca
     7201 agtacaacac gtttttgttg tccgccggat aattctttaa tgtatttagt agtatcatgt
     7261 atacctagtt ttgagaggat tgttttaatt tcagcgttat agtcccaagc atcatgttga
     7321 tccatagcat cttgcgcatc catcattcgc ttgaacaatt tatcactttg gtcatccgca
     7381 taagcttgta ctgcctgctc atattgcttg atgatgcgta aagttgttgt atcagaactt
     7441 aatactgcat caaaaactgt catatcttca ttaaggtcct gtttctgaga cgaatatcga
     7501 attcgatatt gatttggatg cataacattg gctgtaaaat catcatcaat accaccaatt
     7561 acttttaaca acgtactttt ccctgtacca tttatgccta ctaaacctat tttttcacct
     7621 tctgaaattg ataaatctag gttatcgaat atagtcttat cggcataaga tttatttaaa
     7681 tgttcaattt tatatgcttc catacccatc ttccttcttc tgttgaaatc tctacgtact
     7741 taaatcagtt ctatacatta tacacctttt tgaaatcaaa agaaatcgat accgttaagt
     7801 gtcataattt aatatattgt tgcttcaact ataaataaaa cttgttaagg ctttgatgac
     7861 tatgctaatg atctttataa tatacctttt tatcaacaca tataatgtaa aacataatat
     7921 aaaagaacat aataaaaacc cggattgata ttttatcaat ccgagttcgt gtttagatat
     7981 tattttttag agtttgcttt aggtcctgtt tcatatttat acttcgaagg atttaccttt
     8041 ttgaagtctg gatttttgta gaatctaaac aaatcaccat taagcacgtt gtcactcatt
     8101 tcgagatcct tttcaacttg ctttttattc ttttcgaaat cagctggttg agtagttatg
     8161 agttcattat ttttattaga ataaatctta ccattaacat atttataatc ttttgttata
     8221 aagtcaccat ttctgaatgg aactacttga ttatgacctt tagagaataa atcagtaccg
     8281 aacattaaat agttcttcgt atctatacca gccaaatgta aaattgttgg cattacatcg
     8341 acttgaccag catattcatt attgatacca ccagatttac cagggatttt aatccagaaa
     8401 ccagttctgt ttaaatctgt aaatttagcc ggtgtgattt tttcacctaa tagtttttcc
     8461 atggcattgt tatggttttc agagatacca tagtggtcac cataaatcat aatcactgaa
     8521 ttgtcatata atcctttttt cttcaagtca ttaatatatt cttctaatgc ttcgtctaaa
     8581 taacgtgctg tttgaatata accatcaact gttgcatcac ctgtgtttga tttctcaata
     8641 gttgcatcct tttcatctaa tgtgaatgga tagtggttag tcaatgtaat taaatgagaa
     8701 tagaatggtg atttcatctt agcttgataa ttagcagaat ctttaaagaa aattttgtct
     8761 ttcaagccta agtttacaac gtttttatct gacatgtcat agtatgttgc atcatagaat
     8821 ttatcgatac caaagtgttt atatacttgg tctctgttcc agaatgtttt atagtcaccg
     8881 tgcatgacat cagatttgta gccttgcttt tgatctaaaa ttgctggtaa tgactgatac
     8941 gtattatctc cttttaatga aaaggcagaa ccttgcggta aaccgtataa actgttatcc
     9001 attgtaaatt cagagtcaga tgttttacct tgacctgttt gatggaaaaa gttagggaag
     9061 tatgtgaatt gctctttccc acttgataat ttgtttaaaa acggtgttac ttctttacca
     9121 ttaacctttt tattaattaa gaaggtttgg aaactttcta aatgaatctt aataatattt
     9181 ttcttctttg ccaccccata atattctggg ttaggctctg tttgacgttg tttcgtataa
     9241 tttaatactt ttgttaagtc atcttcagat gctagcgctt tttgttgatt attttcgata
     9301 gttttaacac catcgtatac tgtaaagtta taaggtccta aatatttcac taaatattta
     9361 tggtcaaatg tacgtgttaa taattctggt ctgtcagttt cagcaaaagc taagtttaag
     9421 aagaataatg ctactgaagc cgccattacg acaggaacaa atttcttact aaatgctttt
     9481 gtgtctaacc attttgtttt aaatattaaa atgaataagt aaactaacgt atcaatgaaa
     9541 taaacaaagt cataccattt gaatgatgca ctaaccgcac cacccataga ttctacgtta
     9601 cctacttggt ttaaagtact aaacgttaaa aaatcagaga agaatctaaa gtacacaaca
     9661 ttggcatata ataggaacgt caataagaag ccgcctatga acatgaacca aaatgctttt
     9721 ttgcctttaa agaataggaa cacacttaaa accagtgcta ctaaactata aggattcatc
     9781 aataatatta agttttgtac taaaccttta acacctaaag aaaaatcaac ataataagaa
     9841 aaatacgtct tcaaggtaat cgttattacg gttaataaga agaacgcaaa aagactaatt
     9901 ttcttttttt gtgaactcat gattctttcc cccgttattt agataataaa tcttgcgaaa
     9961 gtacttgaat aaattagata ttaacattat gtagttaatt aataagtgcg tttaaaagtt
    10021 aacattgtca taattatagt aattactttc agattcaagt ttataatttt cgttatttgt
    10081 agctttcaac tttgatttaa caagtatttt gggctacaac tcatatttgc ttgtgtaagt
    10141 ttctttattt aatattatca ttcaattaac aaatttaaaa tttgtaatca aattgtaata
    10201 ataagcatag ctcaattaat aaatatagag taattttagt caaaattcaa gtaaaaacac
    10261 actttatctt ttcattttac tgtgaaacga attagaaaat gatacatttc gcagataaaa
    10321 cacagtatta tttcattata cattaggagg aaattatttg catcggactc gagtatgagc
    10381 tacgtaccaa atattatttc atcatttcta attgcattct atattctatt ccatggaata
    10441 attggtttaa ccagttacta tacttaacaa ggttcttttc agccgttaaa atatctataa
    10501 attgaaattt caaacgtgat ccttgcggtt tttgtgctaa tttcgttaaa tgataacttg
    10561 cgattgtacc gatttgcggg tagctaccta gcgtataatg atcatttaat aggataatag
    10621 gtgtgccatc acgcttaatt tgtatcgttc cttttttgac agtctgatac ggtggcatat
    10681 cttcataaaa agctttgatt ttttcacctt caagcatcat ccccatgcga tttgctttgc
    10741 tcgttacttt ataatcatgt ttcacaaatc tttgaatggc atcttcttta aaatcttccg
    10801 tacctttatt tttaacgaca tgaaatacat cagacatata attaaatgac aaggcgtatc
    10861 catcaatacc ccaatctgtt tgtttcgtgt gagcaaggtt ttcaaataac ttatgatgac
    10921 gagctgtata atctctctta agctttattt catcgccatc ttgtaatgtt ctacctttaa
    10981 aaccaccaat ttttacatta aagtcggttg agttagatcc taaccatgca tctaattcaa
    11041 atccgcctcc cacagctaaa tacactcgcg atgtataact tgtttctcta aactttaaaa
    11101 catcgccttt atctaacaaa tacaatttat atggagatat agtcatatgc tcagtgtaag
    11161 ctttgacatt accccctgct aatgcaatca gcgtaggttc tgtaaaacga atcgttgcca
    11221 ttttattagt catttccaaa gttgcttcat tcttgtcatt tgcaactaat cgattagcaa
    11281 tttcatgtgc taaagtatca agtgcaccac atggaattac accatcatgt tcatatcccc
    11341 ttctgccaaa gtcctgaaag ctactgaata agccactttt ttcaattatg attgacatct
    11401 ttaaacgctc ctacttctaa ttcattttca ttgataggtc taaatttaat attatcgcct
    11461 aactttaagc gtgcgaaatc tgattctttc ggattaaaaa gtgataatgg tgtataacca
    11521 ataactaacc aatcattaat tgtatccgta gttacaatac cgcatttttt cccttcaagt
    11581 actacagaac cagctggaat gaattttttc tgtttactcg tgtgattaat atacaatttc
    11641 ttatttaatc cagttaaata aggaaaccca ggtgaatatc ccatcatcga aacaaaatat
    11701 tgcgccttag aatgtaattc aataaaagtt tctagcttga ttttgtaatg ttttaaaagt
    11761 gattctaaat ccggaccata tttcgaacca tacacaattg gtatttcaat caaatcttgc
    11821 tggttaaaat catgttttat gtttaaatga atcgattcta ttagtgcttt catgtataaa
    11881 aaaggtgatt gtatattatg gtgtttaatc atatcccttg cgtcatagac aatcatcatg
    11941 tctgattctg atggcacaat ttctataata aatggataat tttgttcaat taaatatgag
    12001 cgaagtgtta ataaatcttc ggttaaactt ttagatactt ctttttcaat tgcgactaca
    12061 atggcttggt caccttgact atatattttc atttgtacac ctcttcaata tttggttact
    12121 ttactttcat ttttaattat agaatcgatt gaatagttca ttttacctac tcactaaatt
    12181 tactggtggt acattaaatc cattgcccgc acaacaaaag gcataagttc gtgaattaat
    12241 gcgtatacaa ggataaagct tataacagta gtaattgttg ctatcaaacg aacaacatat
    12301 attctatttt cagatagcaa tcgattcact aatcgataat ttacgtatac aagaattaac
    12361 aacagcacaa tataaacaat aatcatattt ggccccattt aatttttaaa tttgttttca
    12421 catcattttc tactttatta tcatactaat tttaaaggca aaggtggaca tcggcaacct
    12481 ctcgtaaact atttatcaaa aataaacgta tctcattgtt atgatattta taaatcaatt
    12541 cgtttttatt aaagtctttt tcaacaagtt tgtcactatc tattaaataa tcacgcatgc
    12601 atccttttaa gaaatcatct ttataacttg gtgtgtacca ttttccatcc tcttcaatga
    12661 caatgttgcc aatatcaaat tcaaggacct tgccgtcctc tgaagttagc aatattaaat
    12721 ctgtttcatg attgtgtgct aaatgctttc gttctgtcgt tttatttttt ataaaagttg
    12781 gattaactac tttgggcaac acttgaaatt ttgctgtaaa actacttttc tcaggtaaag
    12841 gtgacactat cgtttcaaat ttgctttcag catttaatac gattttaagt ctatattgac
    12901 cactgtgata ctttgttgtt acatcgttta attcatttcg ccatgcatgt tcatcaaatt
    12961 taaagtttaa tcgctcagaa gaacatttta tgcgattag
//
LOCUS       DANRDI010000068        12918 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.068, whole genome shotgun sequence.
ACCESSION   DANRDI010000068 DANRDI010000000
VERSION     DANRDI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.068"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(78..1775)
                     /locus_tag="PZ377_001495"
     CDS             complement(78..1775)
                     /locus_tag="PZ377_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDM6358369.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(1775..1993)
                     /locus_tag="PZ377_001496"
     CDS             complement(1775..1993)
                     /locus_tag="PZ377_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDM6358370.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(2022..2144)
                     /locus_tag="PZ377_001497"
     CDS             complement(2022..2144)
                     /locus_tag="PZ377_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358371.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(2474..3025)
                     /locus_tag="PZ377_001498"
     CDS             complement(2474..3025)
                     /locus_tag="PZ377_001498"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDM6358372.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            3390..4016
                     /locus_tag="PZ377_001499"
     CDS             3390..4016
                     /locus_tag="PZ377_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDM6358373.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            4187..5299
                     /gene="pdhA"
                     /locus_tag="PZ377_001500"
     CDS             4187..5299
                     /gene="pdhA"
                     /locus_tag="PZ377_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDM6358374.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            5303..6280
                     /locus_tag="PZ377_001501"
     CDS             5303..6280
                     /locus_tag="PZ377_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDM6358375.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            6371..7663
                     /locus_tag="PZ377_001502"
     CDS             6371..7663
                     /locus_tag="PZ377_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDM6358376.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            7667..9073
                     /gene="lpdA"
                     /locus_tag="PZ377_001503"
     CDS             7667..9073
                     /gene="lpdA"
                     /locus_tag="PZ377_001503"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDM6358377.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            9241..9516
                     /locus_tag="PZ377_001504"
     CDS             9241..9516
                     /locus_tag="PZ377_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDM6358378.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            9660..10199
                     /locus_tag="PZ377_001505"
     CDS             9660..10199
                     /locus_tag="PZ377_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358379.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            10212..11306
                     /locus_tag="PZ377_001506"
     CDS             10212..11306
                     /locus_tag="PZ377_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358380.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            11299..12096
                     /locus_tag="PZ377_001507"
     CDS             11299..12096
                     /locus_tag="PZ377_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358381.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            12102..12911
                     /locus_tag="PZ377_001508"
     CDS             12102..12911
                     /locus_tag="PZ377_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358382.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
BASE COUNT         4544 a         1906 c         2356 g         4112 t
ORIGIN      
        1 catatataaa gaaaaagcta ctaacaatgg gtggtgagtt taattaaaac ttcacttcaa
       61 agttagtagc tttttgatta tttattgttt gattcttttt gttcgtttac cttcataatg
      121 actggtaaaa tcattggttt tctagctgtt ttttcaaata aataaggttg taatgtttca
      181 atgatagaag atttaatctg atgccattga atatctttat tttgatttaa cttactaata
      241 acatcagttt tgattttgcg ttgtgcatca taaattaatt gacctgattc cctcatatat
      301 acaaatcctc gagaaataat gtctggacca gaaagtaatt tatttgtatt aaaatcaata
      361 ctaacaacaa cgataactaa accttcttca gataatagct tacggtctct tattacaaca
      421 ttaccgatat caccgatacc actaccatca acaagtacat taccagatgg aatgcgacca
      481 gctttacgtg ctgaatcgtg tgttaaagct aagacatctc caatatcaaa gatgaagaca
      541 ttatcttctt caacgccgca ttcaacacca gtctcaccat gtgcttttaa catacggtat
      601 tcaccatgaa taggtaagaa atatttcggc ttgattaatc gaagcattaa ttgttgatca
      661 ccttgagaac catgccctga agtatggatg ttagaaatct tgctatggat aacatctgca
      721 ccagctttat acaaggaatt aatagttctg ttaatacttt ttgtattacc tgggataggt
      781 gatgaactaa atacaacggt atcttcaggt ataattttaa tttgcttatg agtaccatta
      841 gcaattctag ataatgctgc cattggttca ccttgtgaac cagtacatag tatcaataac
      901 tcatgcttcg gtacggtatt aattttatta ggttcaataa atgtttcagg tggtgcttta
      961 atataaccaa gttccatacc tattttaata ttgttttcca tcgaacgacc gaacgtaaca
     1021 attttacggt tatttttgat agcagcttca actgcttgtt gaactcggta aatattagaa
     1081 gcgaaggtag caaatataat acgaccttta caattacgga agatcttatc tacgttttga
     1141 ccaacttcac gttcgcttaa agtaaaatca ggcacaagtg aatttgttga gtctgaaagt
     1201 aaacatagaa cgccttcttc gcctaattga gccattttag caatgtttgc tggtttgcct
     1261 acaggtgtaa aatcaaattt aaagtcaccg gtatgaacta cttttccttc aggtgtatct
     1321 acgatgacgc cataagtttc aggaatacta tgtgtagtta agtagaaaga aatcgtaaag
     1381 tgcttagatt taatcacact gtcctcattg atttcattta gtttagcagt acgtaataaa
     1441 tgatgttctt caagtttatt acggattaaa cctaatgcta aaggaccacc ataaataggt
     1501 atattaagtt gttttaatag gaagggcaca ccgcctatat ggtcttcgtg accatgtgtt
     1561 ataaataggc caacaatttt atcttggttt tgaactagat atgtgtagtc aggtataaca
     1621 taatcaatcc ctaataagtt atcatcaggg aatttgatac ctgcatcgat aatgacaatt
     1681 tcgtctttat actcaactgc ataagtattt ttaccgattt cacctagacc tccaagtgca
     1741 tatacaccta cttcatttgg atgtaattgt ttcattattt agcaatctcc acattaaagt
     1801 gttctgagtt ttctttttcg taatctaaat gtgcgccctc taatttagtg ataaattcga
     1861 tattaaaatt acgatctttc aagtaacgac gtacttgttc ttctgtttga gcttcaacat
     1921 aaagtgattg tgtattttca cgcacaatta cctcgtctct gttatgttga taaaaaactt
     1981 taaatactgc catgttaaaa attcctccta agaatgtttg tttaatttat tgttaaccct
     2041 agtaaaatcg tattggagta tatatcgata aattcattcc aatcatctct ataatgtaac
     2101 ttaataacga tttggttaat aactaggttc atcatgtcgt tcattttaaa aaattagtga
     2161 aataacacta aaatttcagt taaatcttat tttacatgat gaatgataat aaataaagca
     2221 gtttatctca ctagaaatct aagtatttcg aataaatgtg cagttctatt tattaaaata
     2281 aatgtagaga ttataaatta atgttaagaa agaattaaag gattttatat gaaacggtaa
     2341 aatttaaatg aggggataaa aagtttggta gaaaggtgaa tatatttaac tgttgtttat
     2401 tcgaaaaagt tgtaaaagga aaagcttagg acatcatatt tatcctaagc tttgataatt
     2461 agaaaaatgt gttttaaact tctactgcat ctgtatgtgg ttgtaatggg tgatctttgt
     2521 caatgtgatc atagaacatt acaccattta aatggtcaat ttcatgttgg aaaacaattg
     2581 ctggatatcc ttttagtcgt aattgtatat cattaccttc gatgtctttg gctttaattg
     2641 taattctatt atgacggtga actagaccag caacattatc atcgacacta aggcaacctt
     2701 caccagttgg taaataagct tcttgaacgc tatgacttac aatttttggg ttcacaagca
     2761 tatagtcata agatttgcca ctgccatcat ctggtattaa aacagcaatc atacgtttag
     2821 aaatattaat ttgaggtgca gccaaaccaa cgcctgaacg taaaccatat cgtttcgcga
     2881 tttcctcatc ttgactattt actaaaaact ctctcatggc gattaatgtt tctttttctt
     2941 ctttagttaa tggtaattct aactcagctg ctttttgacg caaagttgga tgaccatctc
     3001 taatgatgtc tttcattgtt aacatatatt gcaccttcct tattttaatt tgttttagtt
     3061 gaatgacagt aaaaaggttg ttaagatact catacatttt tatgtgtaaa tatctacaaa
     3121 gttaaccaac tactgccaat gtttatttta gatagtatat gtaaattttc agatatgcta
     3181 attgcttaaa aaatgattaa agtgttggtt tcaagcaatg atactttaga aatttattta
     3241 tcatcttgac tttaaaaatt atattataaa tgacgtaact gtcaacagat atacttagta
     3301 atgaagatgt gtaatgtaat tgtttaaaat tgatttccaa gcagatttta tttatcattt
     3361 aatttaaata gcaagtggag gtacaagtaa tgaaatttgg aaaaacaatc gcagtagtat
     3421 tagcatctag tgtcttgctt gcaggatgta ctacggataa aaaagaaatt aaggcatatt
     3481 taaagcaagt ggataaaatt aaagatgatg aagaaccaat taaaactgtt ggtaagaaaa
     3541 ttgctgaatt agatgagaaa aagaaaaaat taactgaaga tgtcaatagt aaagatacag
     3601 cagttcgcgg taaagcagta aaggatttaa ttaaaaatgc cgatgatcgt ctaaaggaat
     3661 ttgaaaaaga agaagacgca attaagaagt ctgaacaaga ctttaagaaa gcaaaaagtc
     3721 acgttgataa cattgataat gatgttaaac gtaaagaagt aaaacaatta gatgatgtat
     3781 taaaagaaaa atataagtta cacagtgatt acgcgaaagc atataaaaag gctgtaaact
     3841 cagagaaaac attatttaaa tatttaaatc aaaatgacgc gacacaacaa ggtgttaacg
     3901 aaaaatcaaa agcaatagaa cagaactata aaaagttaaa agaagtatca gataagtata
     3961 caaaagtact aaataaggtt ggtaaagaaa agcaagacgt tgatcaattt aaataattaa
     4021 tataatacag atggtaggaa acaactaata cagttcctat tatctgtatc tttttttatt
     4081 aaaacagaac tttttcaaat ggtttaacag tcccatttat ttgtggtaca attagtaagg
     4141 ataaaatgaa tttctataca attatgggaa aggtatggtg aattgaatgg ctcctaagtt
     4201 acaagcccaa ttcgatgcag taaaagtttt aaatgatact caatcgaaat ttgaaatggt
     4261 tcaaattttg gatgagaatg gtaacgtcgt aaatgaagac ttagtacctg atcttacgga
     4321 tgaacaatta gtggaattaa tggaaagaat ggtatggact cgtatccttg atcaacgttc
     4381 tatctcatta aacagacaag gacgtttagg tttctatgca ccaactgctg gtcaagaagc
     4441 atcacaatta gcgtcacaat acgctttaga aaaagaagat tacattttac cgggatacag
     4501 agatgttcct caaattattt ggcatggttt accattaact gaagctttct tattctcaag
     4561 aggtcacttc aaaggaaatc aattccctga aggcgttaat gcattaagcc cacaaattat
     4621 tatcggtgca caatacattc aagctgctgg tgttgcattt gcacttaaaa aacgtggtaa
     4681 aaatgcagtt gcaatcactt acactggtga cggtggttct tcacaaggtg atttctacga
     4741 aggtattaac tttgcagcag cttataaagc acctgcaatt ttcgttattc aaaacaataa
     4801 ctatgcaatt tcaacaccaa gaagcaagca aactgctgct gaaacattag ctcaaaaagc
     4861 aattgctgta ggtattcctg gtatccaagt tgatggtatg gatgcgttag ctgtatatca
     4921 agcaactaaa gaagcacgtg accgcgcagt tgcaggtgaa ggtccaacat taattgaaac
     4981 tatgacatat cgttatggtc ctcatacaat ggctggtgac gatccaactc gttacagaac
     5041 ttcagacgaa gatgctgaat gggagaaaaa agacccatta gtacgtttcc gtaaattcct
     5101 tgaaaacaaa ggtttatgga atgaagacaa agaaaatgaa gttattgaac gtgcaaaagc
     5161 tgatattaaa gcagcaatta aagaggctga taacactgaa aaacaaactg ttacttctct
     5221 aatggaaatt atgtatgaag atatgcctca aaacttagca gaacaatatg aaatttacaa
     5281 agagaaggag tcgaagtaag ccatggcaca aatgacaatg gttcaagcga ttaatgatgc
     5341 gcttaaaact gaacttaaaa atgaccaaga tgttttaatt tttggtgaag acgttggtgt
     5401 taacggcggt gttttccgtg ttactgaagg actacaaaaa gaatttggtg aagatagagt
     5461 attcgataca cctttagctg aatcaggtat tggtggttta gcgatgggtc ttgcagttga
     5521 aggattccgt ccggttatgg aagtacaatt cttaggtttc gtattcgaag tatttgatgc
     5581 gattgctgga caaattgcac gtactcgttt ccgttcaggc ggtactaaaa ctgcacctgt
     5641 aacaattcgt agcccatttg gtggtggcgt acacacacca gaattacacg cagataactt
     5701 agaaggtatt ttagctcaat ctccaggtct aaaggttgtt attccttcag gcccatacga
     5761 tgcgaaaggt ttattaattt cttctattag aagtaatgac ccagtcgtat acttagagca
     5821 tatgaaattg tatcgttcat tccgtgaaga agtacctgaa gaagaatata caattgacat
     5881 tggtaaggct aatgtgaaaa aagaaggtaa tgacatttca atcatcacat acggtgcgat
     5941 ggttcaagaa tcaatgaaag ctgcagaaga acttgaaaaa gatggttatt ctgttgaagt
     6001 aattgactta cgtactgttc aaccaatcga tgttgacaca attgtagctt cagttgaaaa
     6061 aactggtcgt gcagttgtag ttcaagaagc acaacgtcaa gctggtgttg gtgcagcagt
     6121 tgtagctgaa ttaagtgaac gtgcaatcct ttcattagaa gcacctattg gaagagttgc
     6181 agcagcagat acaatttatc cattcactca agctgaaaat gtttggttac caaacaaaaa
     6241 tgacatcatc gaaaaagcaa aagaaacttt agaattttaa tacattttaa aagttaacga
     6301 agttagcgta ttttagtctc attgattaaa atgaaatgtt taatttacga aatcttagga
     6361 gggcaaaaac gtggcatttg aatttagatt acccgatatc ggggaaggta tccacgaagg
     6421 tgaaattgta aaatggtttg ttaaagctgg agatactatt gaagaagacg atgttttagc
     6481 tgaggtacaa aacgataaat cagtagtaga aatcccatca ccagtatctg gtactgtaga
     6541 agaagttatg gtagaagaag gtacagtagc tgtagttggt gacgttattg ttaaaatcga
     6601 tgcacctgat gcagaagata tgcaatttaa aggtcatgat gatgattcat catctaaaga
     6661 agaacctgcg aaagaggaag cgccagcaga gcaagcacct gtagctactc aaactgaaga
     6721 agtagatgaa aacagaactg ttaaagcaat gccttcagta cgtaaatacg cacgtgaaaa
     6781 aggtgttaac attaaagcag tttctggatc tggtaaaaat ggtcgtatta caaaagaaga
     6841 tgtagatgca tacttaaatg gtggtgcacc aacagcttca aatgaatcag ctgcttcagc
     6901 tacaagtgaa gaagttgctg aaactcctgc agcacctgca gcagtaacat tagaaggcga
     6961 cttcccagaa acaactgaaa aaatccctgc tatgcgtaga gcaattgcga aagcaatggt
     7021 taactctaag catactgcac ctcatgtaac attaatggat gaaattgatg ttcaagcatt
     7081 atgggatcac cgtaagaaat ttaaagaaat cgcagctgaa caaggtacta agttaacatt
     7141 cttaccttat gttgttaaag cacttgtttc tgcattgaaa aaatacccag cacttaacac
     7201 ttcattcaat gaagaagctg gtgaaatcgt tcataaacat tactggaata tcggtattgc
     7261 agcagacact gatagaggat tattagtacc tgttgttaaa catgctgatc gtaagtctat
     7321 tttccaaatt tcagatgaaa ttaatgaatt agctgttaaa gcacgtgatg gtaaattaac
     7381 agccgatgaa atgaaaggtg ctacatgcac aatcagtaat atcggttcag ctggtggaca
     7441 atggttcact ccagttatca atcacccaga agtagcaatc ttaggaattg gccgtattgc
     7501 tcaaaaacct atcgttaaag atggagaaat tgttgcagcg ccagtattag cattatcatt
     7561 aagctttgac cacagacaaa ttgatggtgc aactggccaa aatgcaatga atcacattaa
     7621 acgtttatta aataatccag aattattatt aatggagggg taaaacatgg tagttggaga
     7681 tttcccaatt gaaacagata ctatagtaat cggagcaggt cctggtggat acgttgcagc
     7741 aattcgtgca gctcaattag gacaaaaagt aacaatcgtt gagaaaggta atcttggtgg
     7801 tgtttgctta aacgtaggat gtattccttc aaaagcatta ctacatgctt ctcaccgttt
     7861 tgttgaagca caacattctg aaaacttagg tgttattgct gaaagtgttt ctttaaactt
     7921 ccaaaaagtt caagaattca aatcatcagt tgttaataaa ttaactggtg gtgttgaagg
     7981 cttacttaaa ggtaacaaag ttaacatcgt taaaggtgaa gcatatttcg tagataacaa
     8041 tagcttacgt gttatggacg aaaagagcgc acaaacatac aactttaaaa atggaatcat
     8101 tgcaacaggt tcaagaccaa ttgaaattcc taatttcaaa ttcggtaaac gtgttatcga
     8161 ctcaacaggt gctttaaact tacaagaagt accaggtaaa ttagttgtag ttggtggagg
     8221 atacattgga tcagaattag gtacagcatt tgctaacttt ggttcagaag taaccatcct
     8281 tgaaggtgct aaagatatct taggtggctt cgaaaaacaa atgacacaac ctgttaaaaa
     8341 aggtatgaaa gaaaaaggtg ttgaaatcgt tactgaagct atggctaaat cagctgaaga
     8401 aacagataac ggagttaaag ttacttatga agctaaaggc gaagagaaaa caatcgaagc
     8461 tgattatgta ttagtaactg taggtcgtcg tccaaacaca gacgaattag gcctagaaga
     8521 attaggtgtt aaattcgctg accgtggatt attagaagtt gataaacaaa gccgtacgtc
     8581 tatcagcaat atctatgcaa ttggtgatat cgttccaggt ttaccacttg ctcacaaagc
     8641 tagctatgaa gctaaagttg ctgctgaagc aattgatggt caagctgctg aagttgatta
     8701 cattggtatg ccagcagtat gctttactga accagaatta gctacagttg gttattcaga
     8761 agcgcaagct aaagaagaag gtttagcaat taaagcttct aaattcccat atgcagcaaa
     8821 tggtcgtgca ttatcattag atgatactaa cggatttgtt aaacttatta cacttaaaga
     8881 agatgatact ttaatcggtg ctcaagtagt tggtactggt gcatcagata ttatctctga
     8941 attaggttta gcaattgaag ctggtatgaa tgctgaagat atcgcattaa caatccatgc
     9001 acatccaaca ttaggtgaga tgactatgga agcagcagaa aaagctatcg gatacccaat
     9061 ccatacaatg taataactga ttatctataa agattcagtc attaaaagct gtagcatatg
     9121 ctacggcttt tttgttttag gtaaagtaat gtaaggaaat tgatttgaga tatcgttaac
     9181 atgtgacatg catgttatac tagcgatgct aacaaaagaa ttgaaatgga gggttcaaca
     9241 atggaatatg agtatccaat tgatttagac tggagtaatg aagagatgat ttcagtgata
     9301 aatttcttta atcatgtaga gaagtattat gaatccggcg tgacggcagg cgactttatg
     9361 ggtgcatata aaagatttaa agaaattgtg cctgctaaag cagaggaaaa acaaattttt
     9421 aatactttcg aaaaaagtag tggctataat agttacaaag cagttcaaga tgtaaaaact
     9481 cactctgaag aacaaagagt aacagctaaa aaataattcg ttcgaaatta acacaattta
     9541 ataggaattt ttctttaaaa ctattgctaa taaagctata ttttgatacc tttatcaagt
     9601 gttaaacaaa atgtttgata aaagtaaact taatatagct tttttaggtg gaaaaataaa
     9661 tgaacatagg taataaaatt aaaaatctta gaagaattaa aaatttaacg caagaagaac
     9721 ttgctgaacg tacagactta tcgaaaggct acatttcaca aatagaaagt gaacatgcct
     9781 caccaagtat ggaaactttc ttaaatatta tagaggtgtt aggaacgacg ccaagtgaat
     9841 tttttaaaga cagtgaaaat gaaaaagtat tatacaagaa ggaagaacaa gttatttatg
     9901 atgagtatga tgaaggttat atattaaatt ggttagtttc aaagtcaaat gaatatgata
     9961 tggagccatt aatattaact ttaaagcctg gagcatcata taaaaatttt aatccatcag
    10021 agtctgatac gtttatttat tgtatgtcag gtcagataac acttaattta ggcaaagaga
    10081 tatatcaagc acaagaagaa gacgttttgt attttaaagc acgagataat catcgtttgt
    10141 caaacgaatc aaacaatgaa acacgaatac ttattgtagc gacagcttca tatttatagg
    10201 ggggatctta tttggaaccg ttattatcat taaaatcagt tagtaaaagc tatgatgatc
    10261 ttaatatctt agatgacata gatattgata ttgaatcagg atacttttat acattattag
    10321 gtccttcagg ttgtggtaaa acaacaattt taaaattaat tgcagggttt gaatatcctg
    10381 acagtggtga agtgatttat caaaacaaac caattggtaa tttaccacca aataaacgta
    10441 aagtgaatac agtctttcaa gattatgcat tatttccaca cttaaacgtc tatgataata
    10501 tcgcttttgg tttgaaatta aaaaaattat caaaaaccga aattgatcaa aaagtaactg
    10561 aggcattaaa attagtaaaa ctttcaggtt atgaaaaaag aaatattaat gaaatgagtg
    10621 gcggacaaaa gcaacgtgtt gcaattgcac gtgctatcgt aaatgaacca gaaatattat
    10681 tgttagatga atctttatcc gcattagatt tgaaattgcg tactgaaatg caatatgaat
    10741 tacgagaatt gcaatctaga ttaggtatta catttatatt tgtaacacat gatcaagaag
    10801 aagcgttagc attaagtgac tttctttttg tattaaaaga tgggaaaatt caacaatttg
    10861 gcacaccaac agatatatat gacgaaccag tgaatcgatt tgtagctgat tttattggag
    10921 aatctaatat tgttgaaggg cgcatggtta gagattatgt cgtgaatatt tatgggcaag
    10981 atttcgaatg tgtcgatatg ggtattcctg aaaataaaaa agtagaagtc gttattcgac
    11041 cagaagatat atcattaatc aaagctgaag aaggattatt taaagcaact gttgattcta
    11101 tgttatttag aggggtccac tatgaaatat gttgtataga caataaaggt tatgaatggg
    11161 taatacaaac gactaaaaaa gctgaagtag gcagtgaagt tggtctttat tttgatcctg
    11221 aagccattca tattatggtt cctggagaaa cagaagaaga atttgataaa cgtattgaaa
    11281 gctatgagga agtagacaat gcgtaatact aataaatttc tcttaatccc gtatttacta
    11341 tggatggtta tatttattat tgtaccagtt gtattactca tttatttttc atttttagat
    11401 atcaatggac attttagttt cacgaattat caacaaattt ttactacaaa atatttgaaa
    11461 atgtttgcat attcaatttt atatgccgct tcaataacaa ttattacctt gactatcagt
    11521 tatccagctg cctattatat tactcgttcg aaatttcaaa atatcttatt aatgataatg
    11581 attattccaa catggataaa tttattgtta aagacatatg cttttatagg tttattaagt
    11641 catgatggcg tgattaatca atttttccac ttatttaatt taccatcatt caatttgtta
    11701 tttacaactg gtgcgttttt agtagtggca agttacattt atataccatt tatgatttta
    11761 cctatattta atagcatgaa agcaattcct aataatttat tgcaggcctc aagtgattta
    11821 ggtgccagtc ctttctatac ttttagaaaa gtaatcatgc cgttaacaaa agaaggtgtt
    11881 atgactggga ttcaagtaac ctttattcca tcactttcat tatttatgat tacaagatta
    11941 attgcaggta ataaagtcat aaatataggt acggcaatag aggaacaatt tttaacaatt
    12001 caaaattatg gtatgggatc aactatagct atattcttaa ttgtatttat ggcattcatt
    12061 ttaatcatta caaaatcatc taatgggaga gggtgaatca tatgaaatgg tatggaaagc
    12121 tgtatatcgg gatactttta gcgattttat acatcccaat attcttttta atgttctatt
    12181 cattcaattc ggctggtaat atgattcact ttgaacattt tacattagag cattatcaat
    12241 cattatttca aaatgatcat ttaatgtcgg tcatttttaa tacgatagct gtagcacttt
    12301 tagcagcctc aatttctaca gttattggta catttggtgc cattgctatt tattatttaa
    12361 gaaataaaaa gtttaaagta actttactaa cattgaataa tgtcttgatg gtatcatccg
    12421 acgttgtcat aggtgcatca ttcttaatta tgtttacaac gattggccat tttactggtc
    12481 ttggtttagg attttggaca gttctaatat ctcatattgc attttgcata cctatagttg
    12541 tgattatcgt cttaccacaa ctgtatgaaa tgaataataa tatgttaaat gctgcaagag
    12601 atttaggagc gactgaacca caattattaa gcaacattat tattcctaat attttacctt
    12661 ctattatagg aggattcttt atggctttaa cttattcact agacgatttc acagtaagtt
    12721 tcttcgttac tggtaatggc tttagtgtgt tatcagttga agtttatgct atggcgagaa
    12781 aaggaattag tatggaaatt aatgcgattt caacattatt gtttgctgtt attgtattag
    12841 gaatactagg atattatttg attcaatacg tgataaataa gaaaaaacta atcaagcgag
    12901 gtgtaaaata atgaaacg
//
LOCUS       DANRDI010000069        12669 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.069, whole genome shotgun sequence.
ACCESSION   DANRDI010000069 DANRDI010000000
VERSION     DANRDI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12669
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.069"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(124..963)
                     /gene="noc"
                     /locus_tag="PZ377_001509"
     CDS             complement(124..963)
                     /gene="noc"
                     /locus_tag="PZ377_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDM6358383.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(1006..1725)
                     /gene="rsmG"
                     /locus_tag="PZ377_001510"
     CDS             complement(1006..1725)
                     /gene="rsmG"
                     /locus_tag="PZ377_001510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDM6358384.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(1725..3602)
                     /gene="mnmG"
                     /locus_tag="PZ377_001511"
     CDS             complement(1725..3602)
                     /gene="mnmG"
                     /locus_tag="PZ377_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDM6358385.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(3669..5048)
                     /gene="mnmE"
                     /locus_tag="PZ377_001512"
     CDS             complement(3669..5048)
                     /gene="mnmE"
                     /locus_tag="PZ377_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDM6358386.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(5187..5540)
                     /gene="rnpA"
                     /locus_tag="PZ377_001513"
     CDS             complement(5187..5540)
                     /gene="rnpA"
                     /locus_tag="PZ377_001513"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDM6358387.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(5661..5798)
                     /gene="rpmH"
                     /locus_tag="PZ377_001514"
     CDS             complement(5661..5798)
                     /gene="rpmH"
                     /locus_tag="PZ377_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDM6358388.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            6529..7890
                     /gene="dnaA"
                     /locus_tag="PZ377_001515"
     CDS             6529..7890
                     /gene="dnaA"
                     /locus_tag="PZ377_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDM6358389.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            8168..9301
                     /gene="dnaN"
                     /locus_tag="PZ377_001516"
     CDS             8168..9301
                     /gene="dnaN"
                     /locus_tag="PZ377_001516"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDM6358390.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            9682..9927
                     /gene="yaaA"
                     /locus_tag="PZ377_001517"
     CDS             9682..9927
                     /gene="yaaA"
                     /locus_tag="PZ377_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDM6358391.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            9924..11036
                     /gene="recF"
                     /locus_tag="PZ377_001518"
     CDS             9924..11036
                     /gene="recF"
                     /locus_tag="PZ377_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDM6358392.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            11046..>12669
                     /locus_tag="PZ377_001519"
     CDS             11046..>12669
                     /locus_tag="PZ377_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIA DNA topoisomerase subunit B"
                     /protein_id="HDM6358393.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQ"
BASE COUNT         4190 a         2206 c         2049 g         4224 t
ORIGIN      
        1 ccagcctgat caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaaagaca
       61 aagctgttat gatcttagct ttgtctttta tgtgttagtc atcatgtata cgacatccta
      121 ctactaacgt ttatatattc gaatttttat ttcataataa tcttcatgat ctttgtcttt
      181 atgctcaaca tgtaatcctg tttgttgaat cgcttggata cttttgccta cctcatctcg
      241 tgcttgagtg acatcttgtg caaagcgaag gttttgtgct ttgacttttt cgggccccgt
      301 tttttggcgt actctatctt cagtctgttt cacatttagc ttttgtgcaa tgacttgctc
      361 aatcaacgct tcttgttcgc tatcagacaa tgataatacc gctctcgcat gacgttcagt
      421 aattttacct tctcttaagc gaagtagtac tttcggcgcc aacttcaata aacgcaactt
      481 atttgcaata aagctttgac ttttacctaa actttttgcc aattcacttt gcgttgtatc
      541 accaatttcc aataatttct tataggcttc cgcttcttca acaacagaca aattttctct
      601 ttgaatattc tcaattaatg caacaacagc cgtctcttca tcatccatat cacgaataat
      661 aacgtctgct tgaggtaaat ttagtgattg tattgctcta aatcggcgct ctccagcaat
      721 aatttcaaac atatcttctt caatcggtct tacaacaata ggttgtagta aaccatgttc
      781 atgtattgat tcagcaagtt ctttaatttt atttggttca aacacctgtc ttggttgata
      841 acggttggga acgatacgtt caatttgaat ggattcaaca ttactattgc gatcttcttc
      901 aatatgtcca atgatgtcat ctttgttttt caaaccaaat aattttgaaa aaggtttttt
      961 cattatccat tcgctccttt tatgttagtt gtaaaggatt atgcattatt tttcaagtaa
     1021 aggagtctta ttaggcgtcc ctggttttct tggatacttt ttcggcgtct gtcttttttt
     1081 atcaataatg aacatctggc gctctccagc atcttctggc aattcaaagg tatgtgtttc
     1141 tgtaacatta ccacctaaca cactaattgc aaattttgct tcttctaatt cttcttcacc
     1201 ttttgaagat tttaatgcaa caaactgtcc acctttttta actagcggta aacacaattc
     1261 acttaacacg gataatctag ctactgctct tgcagtaaca acatcataag actccctgta
     1321 gacaccctta ccaaatgttt ctgctctatc gtgtataaag ctgacatcct gtaattgtaa
     1381 ttctgacgct aaatggttta aaaattgaat acgcttattt aatgaatcaa caatcgtcac
     1441 ttttaactgc ggaaacatta tttttaacgg aatacttgga aaaccagctc cagcgcctac
     1501 atcacatata cttataggct gattaaaatc aaaataaaaa ctaggtgcaa tggaatcata
     1561 aaaatgtttc aaatatacat cgtgttcatc tgtaatactt gtcaaattca tcttttcatt
     1621 ccattcaaca agtaaacgat aatatgtttg aaactgttgt ttttgagtct cagttaattg
     1681 aatattatgt tcttttaatt gttctgctaa ccattctaca gtcattagtc actcaccctt
     1741 tggagtttac cttgttctaa gtaaatcaat aatatagata tgtctgctgg atttacccct
     1801 gatattctag aagcttgtgc aatatttaaa ggttttactt ctgataattt ttctcgcgct
     1861 tcagtcgcca aactatcaat cttactataa tctaagtctt ctggaatttt cttctcttcc
     1921 atacgcttaa ctttctcaac ttgttgtagt gatttattga tataaccttc atattttgtt
     1981 tgtatttcta cttgttcttc aacatctgca ttcaattgat gttcttcttc taaaagttct
     2041 aaaattatat cgtaagtcat ttcaggtctg cgtaataaat cgatagctaa aataccatct
     2101 tttaagcgag aaccaccatg ttgttcaata atcgcttgcg tatgttcgtt tggtttaata
     2161 cgaatatctg ataaacgctt aatttccgca tcaatttgct gacgtttttc attaaaacgt
     2221 gcatatcttt cttcagaaat cataccaagt tcatatccca tatccgtcaa tctcaaatca
     2281 gcattatcat gacgtagtaa caaacgatat tctgcacgtg atgttagtaa acgataaggt
     2341 tcattagtac ctttagttac aagatcatcg attaagacac caatatatgc atctgaacga
     2401 cttaatatct tttcgcctgt gtttaacact ttacctgcag cgttaatacc tgccatcaat
     2461 ccttgtcctg ctgcttcttc ataaccagat gtaccattaa tttgacctgc agtatataag
     2521 tttttaatca ttttcgtttc aagtgtaggc cataactgcg ttggcacaat cgcatcatat
     2581 tcaattgcgt agccggcacg catcatatct gctttttcaa gacctggtat cgtctctaac
     2641 atttgacgtt gcacatgttc aggaagactt gtagacaatc cttgcacata tacttcattt
     2701 gtattacgac cttcaggctc taagaaaagt tgatgtcgcg gcttatcatt aaatcgaaca
     2761 aatttatctt caattgaagg gcaataacgt ggcccggttc ctttaatcat ccctgaatac
     2821 attgcagata gatgtaaatt atcatcgata actttgtgtg tttcagcatt agtatacgtt
     2881 agccaacatg gcaattgatc taatatatat tctgttgttt caaagctgaa tgcacgacct
     2941 acatcgtcac ctggttgtat ttcagtcttc gaatagtcaa ttgtttttga atttacacgc
     3001 ggtggtgtac ctgttttaaa acgaacaata tcaaaaccaa gttctcttaa attgtctgat
     3061 aatgtgattg atggtaattg gtgatttggt ccacttgaat acttcatatt acctaaaatg
     3121 atttcaccac gtaaaaatgt tcccgttgta ataattactg ctttagataa atactctgta
     3181 ccaatatttg tacgtacacc tttaacttca ttatcttcta taataagttc gtctaccata
     3241 ccttgcatta tatgcaaatt ttcttcatct tcaatcacgc gtttcatttc ttgttgataa
     3301 agtactttat ctgcttgcgc tcttagtgct cttacagcag gtcctttacc tgtatttaac
     3361 attctcattt gaatgtgtgt tttatcgatt gtttttgcca tttgtccacc taaagcatca
     3421 atttcacgaa caacgatacc tttagctggt ccacctacag atgggttaca tggcataaat
     3481 gcaatattat ctaaatttat tgttagcatt aatgttttag caccacgtct tgcagatgct
     3541 aaacctgctt ctacacctgc atgtcccgca cctataacga ttacatcata ttcttgaacc
     3601 acaatataaa cctccttatt tgatatctta ctagccttct taagacggta ttccgtctat
     3661 ttcaattact atttacctaa gcagaattga ctgaataact gatcgatgag ttcatcactt
     3721 gcagtctcac caataatttc tcctaatatt tcccaagttc tagttaaatc aatttgtacc
     3781 atatccatag gcacaccaga ttctgctgca tcaatcgcat cttgtatcgt ttgtcttgct
     3841 tgttttaata atgaaatatg tcttgaatta gaaacataag tcatatcttg attttgtact
     3901 tctccaccaa agaacaaatc tcgaatttgt atttctaatt catcaatacc ttcttgtttt
     3961 aacattgaag tttgaattaa tggcgtatca cctatcatat ctttaacttc attaatatct
     4021 atgttttgct ctaaatccat tttattaaca attacgatta catcttcatt tttaaccact
     4081 tcatataatg tgtaatcttc ttgagtcaat gcttcgttat tgtttaatac aaataaaatt
     4141 aagtctgctt ggctaagagc ctttctagag cgttcaacac caatcttctc tactatatct
     4201 tctgtctcac gtataccagc agtatcaact aatcttaatg gcacgccacg aacattgacg
     4261 tactcttcta agacatctct agtagtacct gctacctcag ttacaatcgc tttattatct
     4321 tgtattaaat tatttaacat cgatgattta cctacgtttg gtttaccaac aataactgta
     4381 gataaacctt cacgcataat tttaccctgc gcaccggtat ctaataaacg attaatttcc
     4441 tgtttgattt ctttagactg ctctaaaaga aattcagtag tcgcatcttc aacatcatcg
     4501 tattcaggat aatcaatatt cacttccact tgagcgagta tctctaatat agattgacgt
     4561 tgttttttga ttaagtcact tagacgacct tcaatttgat tcatcgcaac tttagaagct
     4621 ctatctgtct tcgagcgaat aaagtccata actgcttcag cttgagataa atcaatacga
     4681 ccatttaaaa aggcacgttt tgtaaattca cctggctcag ccattctagc gccatatgtc
     4741 atagtaagtt ccagcactct attaatcgtt aaaataccac catgacaatt aatttctata
     4801 atatcttcgc gtgtaaatgt ttttggcgct cttaacacag acaccataac ttcttcaatc
     4861 acttctttag actctggatc aataatatga ccgtaattaa tcgtatgtga tggaacatca
     4921 tttaaaagat gttttccttt atataatttg tcagcaattt caacggcttg cggtccagac
     4981 aatcgaacaa ttccaattgc cccttcaccc attggtgttg aaatactcgt aattgtatct
     5041 aaatccatat tgctactcgc ctccttcaac gatgtgaata cattttaaag taagttatta
     5101 taaccctaag gtcagtctta cgtttgtctg aggtaagact tcgggatgtg ttgagtggtt
     5161 aatgttttcc ttcccctacc ctatccttac ttaatctttt tattaaaaac tttggcaatt
     5221 ttaagtacgt gctcaagact attctgtatt tgtaaagtcg tcatatcttt agctggctgt
     5281 cttgctatta caataatatc tttggccaat atatgcgact tatgtacttt gaaattttca
     5341 cgtattgctc ttttaatctt gtttcttaac actgcattac ctagtttttt agaaacacta
     5401 atacctaagc gaaaatggtc tatttcttta ttattacaag tgtatacaac aaattgtctg
     5461 ttggctacag aatgaccttt tttatatatt ctctgaaaat ctgcattctt tttaattcgg
     5521 taagcttttt ccaataacat cactcgctta tttatcgttt ttatttgaag ctatatttaa
     5581 acttctattg agcttataac ataaatttct atttattctt aatttaaacg aaaaaaaaga
     5641 tcactgatga gtcagtgatc ttatgcagat aaaactttac ggcctttacg acgacggcgc
     5701 gctaaaactt tacggccatt ttttgtgctc atgcgttttc tgaaaccatg aactttacta
     5761 tgtttacgtt tatttggttg ataagtacgt tttaccatgc aaaacacctc catctttata
     5821 cagttatcta atataccaga ttatgcagac tctgatcact ttaatagata agattttgcg
     5881 tttcttagat taaaattctc gtattagctc attgattatc tagtcataat tcaagcaact
     5941 actacaatat aacaaaatcc tatttataac gcaagttcat tttatactac tgctcaattt
     6001 ttttactttt atcgattaaa gatagaaata cacgatgcga gcaatcaaat ttcataacat
     6061 caccatgagt ttggtccgaa gcatgagtgt ttacaatgtt cgaacacctt atacagttct
     6121 tatacatact ttataaatta tttcccaaac tgttttgata cactcactaa cagatactct
     6181 atagaaggaa aagttatcca cttatgcaca tttatagttt tcagaattgt ggataattag
     6241 aaattacaca caaagttata ctatttttag caacatattc acaggtattt gacatataga
     6301 ggactgaaaa agtataattg tgtggataag tcgtccaact catgatttta taaggattta
     6361 tttattgatt tttacataaa aatactgtgc ataactaata agcaagataa agttatccac
     6421 cgattgttat taacttgtgg ataattatta acatggtgtg tttagaagtt atccacggct
     6481 gttatttttg tgtataactt aaaaatttaa gaaagatgga gtaaatttat gtcggaaaaa
     6541 gaaatttggg aaaaagtgct tgaaattgct caagaaaaat tatcagctgt aagttactca
     6601 actttcctaa aagatactga gctttacacg attaaagatg gtgaagctat cgtattatcg
     6661 agtattcctt ttaatgcaaa ttggttaaat caacaatatg ctgaaattat ccaagcaatc
     6721 ttatttgatg ttgtaggcta tgaagttaaa cctcacttta ttactactga agaattagca
     6781 aattatagta ataatgaaac tgctactcca aaagaaacaa caaaaccttc tactgaaaca
     6841 actgaggata atcatgtgct tggtagagag caattcaatg cccataacac atttgacact
     6901 tttgtaatcg gacccggtaa ccgctttcca catgcagcga gtttagctgt ggccgaagca
     6961 ccagccaaag cgtacaatcc attatttatc tatggaggtg ttggtttagg aaaaacccat
     7021 ttaatgcatg ccattggtca tcatgtttta gataataatc cagatgccaa agtgatttac
     7081 acatcaagtg aaaaattcac aaatgaattt attaaatcaa ttcgtgataa cgaaggtgaa
     7141 gctttcagag aaagatatcg taatatcgac gtcttattaa tcgatgatat tcagttcata
     7201 caaaacaagg tacaaacaca agaagaattt ttctatactt ttaatgaatt gcatcagaat
     7261 aacaagcaaa tagttatttc gagtgatcga ccaccaaagg aaattgcaca attagaagac
     7321 cgattacgtt cacgctttga atgggggcta attgttgata ttacgccacc agattatgaa
     7381 actcgaatgg caattttgca gaagaaaatt gaagaagaaa aattagatat tccaccagaa
     7441 gctttaaatt atatagcaaa tcaaattcaa tctaatattc gtgaattaga aggtgcatta
     7501 acacgtttac ttgcatattc acaattatta ggaaaaccaa ttacaactga attaactgct
     7561 gaagctttaa aagatatcat tcaagcacca aaatctaaaa agattaccat ccaagatatt
     7621 caaaaaattg taggccagta ctataatgtt agaattgaag atttcagtgc aaaaaaacgt
     7681 acaaagtcaa ttgcatatcc gcgtcaaata gctatgtact tgtctagaga gcttacagat
     7741 ttctcattac ctaaaattgg tgaagaattt ggtgggcgtg atcatacgac cgtcattcat
     7801 gctcatgaaa aaatatctaa agatttaaaa gaagatccta tttttaaaca agaagtagag
     7861 aatcttgaaa aagaaataag aaatgtataa gtaggaaact ttgggaaatg taatctgtta
     7921 tataacagca ctaatgataa caatcatttt ttacatttct atatgctaat gtggcaagat
     7981 gagcaaaact cattttgtgg ataatgttta aaagtcatac acaccataca caagttatca
     8041 acatgtgtat aacttcgcca aatctatgtt tttaagactt atccaccaat ccacagcacc
     8101 tactactatt actaagaact taaaacctat ataattatat ataaacgact ggaaggagtt
     8161 ttaattaatg atggaattca ctattaaaag agattatttt attacacaat taaatgacac
     8221 attaaaagct atttcaccaa gaacaacatt acctatatta actggtatca aaatcgatgc
     8281 gaaagaacat gaagttatat taactggttc agactctgaa atttcaatag aaatcactat
     8341 tcctaaaact gtagatggcg aagatattgt caatatttca gaaacaggct cagtagtact
     8401 tcctggacga ttctttgttg atattataaa aaaattacct ggtaaagatg ttaaattatc
     8461 tacaaatgaa caattccaga cattaattac atcaggtcat tctgaattta atttaagtgg
     8521 cttagatcca gatcaatatc ctttattacc tcaagtttct agagatgacg caattcaatt
     8581 gtcggtaaaa gtgcttaaaa acgtgattgc acaaacaaat tttgcagtgt ccacctcaga
     8641 aacacgccca gtactaactg gtgtgaactg gcttatacaa gaaaatgaat taatatgcac
     8701 agcgactgac tcacaccgct tggctgtaag aaagttgcag ttagaagatg tttctgaaaa
     8761 caaaaatgtc atcattccag gtaaggcttt agctgaatta aataaaatta tgtctgacaa
     8821 tgaagaagac attgatatct tctttgcttc aaaccaagtt ttatttaaag ttggaaatgt
     8881 gaactttatt tctcgattat tagaaggaca ttatcctgat acaacacgtt tattccctga
     8941 aaactatgaa attaaattaa gtatagacaa tggggagttt tatcatgcga ttgatcgtgc
     9001 ctctttatta gcgcgtgaag gtggtaataa cgttattaaa ttaagtacag gtgatgacgt
     9061 tgttgaattg tcttctacat caccagaaat tggtactgta aaagaagaag ttgatgcaaa
     9121 cgatgttgaa ggtggtagcc tgaaaatttc attcaactct aaatatatga tggatgcttt
     9181 aaaagcaatc gataatgatg aggttgaagt tgaattcttc ggtacaatga aaccatttat
     9241 tctaaaacca aaaggtgacg actcggtaac gcaattaatt ttaccaatca gaacttacta
     9301 aaaataaata taaataaagg atgacgtgat taattaaaac gtcatccttt attttttggc
     9361 aaaaataatt ctaggtgcgt atgtaaaata aatttggcag cattttaaac agcaaataaa
     9421 agacgccaat taaatttatg acaaatgtat ccaaaattta ataagtgtgc ttatatgccc
     9481 tttaaattta aaattttaat agtcaataac aagttgaata taaaagttaa acgccgttaa
     9541 atagcgttaa aaaattgaaa atgacagtat tgccaaaaaa taagaattaa ttatttatat
     9601 gtaaacggtt tctacctcta ttttaaatga aatttgtgac aaaaaaaggt ataatatatt
     9661 aatgacatac aaagaaatgg agtgattatt ttggttcaag aagttgtagt agaaggagac
     9721 attaatttag gtcaatttct aaaaacagaa gggattattg aatctggtgg tcaagcaaaa
     9781 tggttcttgc aagacgttga agtattaatt aatggagtgc gtgaaacacg tcgcggtaaa
     9841 aagttagaac atcaagatcg tatagatatc ccagaattac ctgaagatgc tggttctttc
     9901 ttaatcattc atcaaggtga acaatgaagt taaatacact ccaattagaa aattatcgta
     9961 actatgatga ggttacgttg aaatgtcatc ctgacgtgaa tatcctcatt ggagaaaatg
    10021 cacaaggaaa gacaaattta cttgaatcaa tttatacctt agctttagca aaaagtcata
    10081 gaacgagtaa tgataaggaa ctcatacgtt ttaatgctga ttatgctaaa atagaaggtg
    10141 agcttagtta tagacacggc acgatgccat taacaatgtt tataactaaa aaaggtaaac
    10201 aagtcaaagt gaatcactta gagcaaagtc gtctaactca atatattgga cacctcaatg
    10261 tggttctatt tgcgccagaa gatttgaata ttgtaaaagg ctctcctcaa ataagacgac
    10321 gctttataga tatggagttg ggccaaattt ctgctgttta cttaaatgat ttagctcaat
    10381 accaacgtat tttaaagcaa aagaataatt acttaaagca gttacaatta ggccaaaaaa
    10441 aggacttaac aatgttggaa gtattaaatc agcagtttgc tgaatatgca atgaaagtaa
    10501 ctgataaacg tgcacatttt attcaagagc tagagtcgtt agctaaaccg attcatgctg
    10561 gtatcacaaa tgataaagaa gcgttgtcgc tgaattattt acctagtctt aaatttgatt
    10621 atgctcaaaa tgaagcggca cgacttgaag aaattatgtc tattcttagc gataatatgc
    10681 aaagagaaaa agaacgaggc attagcttat tcggaccaca tcgagatgat ataagttttg
    10741 atgtgaatgg catggatgct caaacatatg gttctcaagg acagcaacgt acaacggctt
    10801 tgtccattaa attagctgaa attgagttaa tgaatatcga agttggggaa tatcccatct
    10861 tattattaga cgatgtactc agtgaattag atgattcgcg tcaaacgcat ttattaagta
    10921 cgattcagca taaagtacaa acatttgtca ctacgacatc tgtagatggt attgatcatg
    10981 aaatcatgaa taacgctaaa ttgtatcgta ttaatcaagg tgaaattata aagtaacaga
    11041 aagcgatggt gactgcattg tcagatgtaa acaacacgga taattatggt gctgggcaaa
    11101 tacaagtatt agaaggttta gaagcagtac gtaaaagacc aggtatgtat ataggatcga
    11161 cttcagagag aggtttgcac catttagtgt gggaaattgt cgataatagt atcgatgaag
    11221 cattagctgg ttatgcaaat caaattgaag ttgttattga aaaagataac tggattaaag
    11281 taacggataa cggacgtggt atcccagttg atattcaaga aaaaatggga cgtccagctg
    11341 tcgaagttat tttaactgtt ttacatgctg gtggtaaatt tggcggtggc ggatacaaag
    11401 tatctggtgg tttacatggt gttggttcat cagttgtaaa cgcattgtca caagacttag
    11461 aagtatatgt acacagaaat gagactatat atcatcaagc atataaaaaa ggtgtacctc
    11521 aatttgactt aaaagaagtt ggcacaactg ataagacagg tactgtcatt cgttttaaag
    11581 cagatggaga aatcttcaca gagacaactg tatacaacta tgaaacatta cagcagcgta
    11641 ttagagagct tgctttctta aacaaaggaa ttcaaatcac attaagagat gaacgtgatg
    11701 aagaaaacgt tagagaagac tcctatcact atgagggcgg tattaaatcg tacgttgagt
    11761 tattgaacga aaataaagaa cctattcatg atgagccaat ttatattcat caatctaaag
    11821 atgatattga agtagaaatt gcgattcaat ataactcagg atatgccaca aatcttttaa
    11881 cttacgcaaa taacattcat acgtatgaag gtggtacgca tgaagacgga ttcaaacgtg
    11941 cattaacgcg tgtcttaaat agttatggtt taagtagcaa gattatgaaa gaagaaaaag
    12001 atagactttc tggtgaagat acacgtgaag gtatgacagc aattatatct atcaaacatg
    12061 gtgatcctca attcgaaggt caaacgaaga caaaattagg taattctgaa gtgcgtcaag
    12121 ttgtagataa attattctca gagcactttg aacgattttt atatgaaaat ccacaagtcg
    12181 cacgtacagt ggttgaaaaa ggtattatgg cggcacgtgc acgtgttgct gcgaaaaaag
    12241 cgcgtgaagt aacacgtcgt aaatcagcgt tagatgtagc aagccttcca ggtaaattag
    12301 ccgattgctc tagtaaaagt cctgaagaat gtgagatttt cttagtcgaa ggggactctg
    12361 ccggggggtc tacaaaatct ggtcgtgact ctagaacgca ggcgatttta ccattacgag
    12421 gtaagatatt aaatgttgaa aaagcacgat tagatagaat tttgaataac aatgaaattc
    12481 gtcaaatgat cacagcattt ggtacaggaa tcggtggcga ctttgatcta gcgaaagcaa
    12541 gatatcacaa aatcgtcatt atgactgatg ccgatgtgga tggagcgcat attagaacat
    12601 tgttattaac attcttctat cgatttatga gaccgttaat tgaagcaggc tatgtgtata
    12661 ttgcacagc
//
LOCUS       DANRDI010000070        12627 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.070, whole genome shotgun sequence.
ACCESSION   DANRDI010000070 DANRDI010000000
VERSION     DANRDI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.070"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            299..3004
                     /gene="acnA"
                     /locus_tag="PZ377_001520"
     CDS             299..3004
                     /gene="acnA"
                     /locus_tag="PZ377_001520"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDM6358394.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3184..3651
                     /locus_tag="PZ377_001521"
     CDS             3184..3651
                     /locus_tag="PZ377_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDM6358395.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3858..4154)
                     /locus_tag="PZ377_001522"
     CDS             complement(3858..4154)
                     /locus_tag="PZ377_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDM6358396.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4530..5138)
                     /gene="plsY"
                     /locus_tag="PZ377_001523"
     CDS             complement(4530..5138)
                     /gene="plsY"
                     /locus_tag="PZ377_001523"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDM6358397.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5344..7335
                     /gene="parE"
                     /locus_tag="PZ377_001524"
     CDS             5344..7335
                     /gene="parE"
                     /locus_tag="PZ377_001524"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDM6358398.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7335..9737
                     /gene="parC"
                     /locus_tag="PZ377_001525"
     CDS             7335..9737
                     /gene="parC"
                     /locus_tag="PZ377_001525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDM6358399.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDYSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9987..11447
                     /locus_tag="PZ377_001526"
     CDS             9987..11447
                     /locus_tag="PZ377_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDM6358400.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
     gene            11947..>12627
                     /locus_tag="PZ377_001527"
     CDS             11947..>12627
                     /locus_tag="PZ377_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDM6358401.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHA"
BASE COUNT         4455 a         1777 c         2377 g         4018 t
ORIGIN      
        1 aaaagaatga cattgaaaaa aatattaaag tttggtgcca taattttctt tctgctcaat
       61 gaaataatac gtttaaaaat tgaattgaat cattcgcgaa attgataatt attctcaatt
      121 aataaaaaat acttattaca ccttacctgt atgataagtt ttgcttatat actctgatta
      181 aaaagtcaaa acctaaatta agcgttttct aagcatgatg agttgtttat caataaatat
      241 aataatacaa tttaatcaag gcatataaat ataaaaatgt atcaaggggg atcattaaat
      301 ggctgcaaat tttaaagagc aatcaaaaaa acattttgac ttgaatggcc aaagttatac
      361 ttactatgat ttaaaagctg tagaagagca aggtattact aaagtttcca atttacctta
      421 ttcaattcgt gttttgttag aatctttact tcgtcaagaa gatgattttg taattacaga
      481 cgatcatatt aaagctttaa gtcagtttgg aaaagatgga aatgaaggcg aggtgccatt
      541 taaaccttct cgtgttattt tacaagattt cacaggtgta ccagccgtag ttgatttagc
      601 ttctttacgt aaagcaatgg atgacgttgg gggagatatt actaaaatta atccagaagt
      661 accggtggat ttagttattg accactcagt tcaagtggat agctatgcaa atccagaagc
      721 tcttgaacgt aatatgaaat tagaatttga acgtaactat gaacgttatc agtttttaaa
      781 ttgggcaacg aaagcatttg ataattacaa tgcagttcct cctgcaactg gaatagttca
      841 ccaagttaac ttagaatatt tagcaagtgt tgtacatgtt cgtgatgtag atggtgaaaa
      901 aactgcattt ccagatacat tagttggtac tgattcacat acaacaatga taaatggtat
      961 tggcgtacta ggatggggtg ttggtggtat tgaagctgaa gctggaatgc ttggacaacc
     1021 ttcttatttc ccaattccag aggttattgg tgtacgacta gtaaattcat taccacaagg
     1081 cgcaacagca actgatttag cgttaagagt aactcaagag ctacgtaaaa aaggtgttgt
     1141 tggtaaattt gtggagttct ttggtccagg tgtacaacat ttaccactag cagaccgtgc
     1201 tacaattgca aacatggcac cagagtatgg agcaacttgc ggattcttcc cagttgatga
     1261 tgaatctctt aaatatatga agttaactgg tagatcagac gaacatatcg cgctagtaaa
     1321 agaatatttg aaacaaaacc atatgttctt tgatgttgag aaagaagatc ctaattatac
     1381 agatgttatc gaattggatt tatcaacagt tgaagcatcg ctttcaggac caaaacgtcc
     1441 tcaagattta attttcttaa gtgatatgaa atcatcattt gaaaattctg taacagctcc
     1501 agcaggcaac caaggacacg gtttagataa aagtgaattt gataagaaag ctgaaattaa
     1561 ctttaaagat ggatcaaaag ctacaatgaa aacaggtgat attgcaatag cagcaattac
     1621 atcatgtaca aatacatcta acccttatgt aatgttaggt gcaggtttag ttgctaaaaa
     1681 agcagttgaa aaaggcttga aagttcctga atacgttaaa acttctctag caccaggatc
     1741 aaaagttgtt accggatatt taagagatgc tggcttacaa ccttatttag atgatttagg
     1801 cttcaacttg gttggttatg gatgtacaac ttgtatcggt aattcaggtc ctttattacc
     1861 agaaattgaa aaagcgattg ctgatgagga cctattagtg acatctgtat tatctggtaa
     1921 ccgtaacttt gaaggtcgta tccatcctct tgttaaagcc aattacctag cttcaccaca
     1981 gttagttgtt gcttatgcat tagctggaac ggttgatatt gatttacaaa atgaacctat
     2041 tggtaaaggt aatgacggtg aagatgtata tttgaaagat atttggccat caattaaaga
     2101 agtttcagat accgttgata gtgttgtaac acctgaatta tttattgaag aatataataa
     2161 cgtatacaat aacaacgaat tatggaatga gattgatgta actgatcaac ctctatatga
     2221 ctttgatcct aattcaacat acattcaaaa tccatcattc ttccaaggat tatctaaaga
     2281 accgggtacg attgttccat taaatggttt acgtgttatg ggtaaattcg gtgattctgt
     2341 gacaactgac cacatctctc cagcaggtgc aattggtaaa gatacgccag ctggtaaata
     2401 tttacaagat catcaagtgc ctattcgtga atttaattca tatggttcaa gacgtggtaa
     2461 tcacgaagta atggttcgag gtacgtttgc taatatacgt attaaaaacc aattagcgcc
     2521 aggtactgaa ggtggtttta caacttattg gccaacaaat gaagtaatgc ctatctttga
     2581 tgctgcaatg aaatataaag aagatggtac aggtttagtt gtattagctg gtaacgatta
     2641 tggtatgggt tcatctcgtg actgggcagc aaaaggtaca aacttattag gtgttaaaac
     2701 agttattgca caaagttatg aacgtatcca tcgttcaaat ttagttatga tgggtgtatt
     2761 accattagag tttaaaaaag gtgaatcagc tgattctctt ggtctagatg gtacagaaga
     2821 aatttctgtt aatattgatg aaaatgttca accacatgac tacgtcaaag ttactgctaa
     2881 gaagcaagat ggtgatttgg tagaatttga cgctatggtt cgttttgact cacttgttga
     2941 aatggattac tatcgtcacg gtggaatttt acaaatggtt ttaagaaata aattagcgca
     3001 ataaaaaata gatatcacag taaaatttta atcggtattt gaggcacttt gatagtgctc
     3061 aaaaatactt ctataaactt tatgatataa taggtcggtt caaaaccttt gtgttcttgg
     3121 ccggcctctt cttgtatttt atgttagaat gagatataaa gttaaactga aagaaggctt
     3181 aaaatgatat atagtattac agaaatagaa gcgcgttatg ctgaaactga taagatgggt
     3241 gtaatttatc acgggaatta tgcaacttgg tttgaagttg cgcggttgga ttatatatcg
     3301 aagttaggtt ttagttatgc tgatatggaa aaacaaggaa tcatttcacc tgtgactgac
     3361 ctcaatgtca attataaaaa gtctattttt tatccagaaa aagttaaagt taaaacttgg
     3421 gttgaaaaat attcgagatt acgttcagtg tataaatatg agatttttaa tgaaaaaggt
     3481 gaacttgcaa ctacaggttc cacagaattg atttgcatta aagaagatac ttttaagcct
     3541 atacggttgg atcgttattt cccagattgg catgaagctt atagtaaagt gcaaacgctc
     3601 aataatgaag ggaaaacagt agagataatg gatggtattg attctttata agttactgta
     3661 aatgtttgga gaggcaatat taacaattaa tctataaaaa atagccaata cgttaacatt
     3721 aatgtaatta gcgtgaagat aagctaatat atagcaaaaa cgaacttgtt catacaacat
     3781 cagatgtgct aaaccaaagt acaacttccg aggttcagga cacatcagaa tgtgtaaaca
     3841 agttcgtttt attttagtta ttttgtggaa taagaaattt catcttcgtg atcaactaca
     3901 tttacaataa tgtggtcatc ttcaaagtac cacaaatctt tttccgctac gacaacattt
     3961 aaatcgtcat attgttgttc atagccaata tcaacatctt cctttggttc aactgtaaaa
     4021 gcaggactaa atccttgctt gagttggaat tcgccaccat atcttacaaa aaacacgagc
     4081 actttattat tttcaggcaa ctcaagttca tttttaaacc aagttactgc tgcatcagta
     4141 agttctattt gcataacaat gacctcctat atttattaca gaatgggagt gggccaactt
     4201 gtattatttg tggaatttct tatcgaaatt ctctgtgttg gagcccaccc caacctgctc
     4261 attattgtaa gctgaatttc cataagcttc tatgttggga cccccgacca gaattgaaaa
     4321 aacctagtta taagcgcatt ttgttcggtt aactacagct aatataacat tgtggagcct
     4381 atgacattga tttatgtctc ggtctcaaat gttcttgtaa aaaaactaag tatagttgaa
     4441 ttttaagtta aaaaaattgt ttttgcaaaa ataaaataat ccatcaactg caaaaaaata
     4501 tgcaatgatg gactaatgtc aaaaaattgt tacatccatt ttattttagg ttcttcgcct
     4561 ctaaaaatcc ttgcgatatt agagcgatgt ctaattatca atatgattga aactaagaaa
     4621 ctaacgacta ataaaatata gtcttgaatg ataagcgagc caatcacaca gcaaattgct
     4681 gcaacgatac ttgctaaaga aacatattta aaaatcttca atacaataaa gaagataatt
     4741 gcaagtatta gtaaaagtat cggattgact cccaagacga cacctgcact agttgcaaca
     4801 gctttgccac cttggaattt taaataaaca ggataaacgt gtccaagtat agcgaataag
     4861 ccaacaatta aaccatttgt aaaaaaagta ctaatagggc catctgcgtg aacttgtaac
     4921 cataaaggga agaaaacagt tatgaaccct ttgaaaatat ctagaaatgt taccaagaat
     4981 cctgcaggac gacctaatac tctaaagcta ttagtagcgc cagtattacc actaccaaat
     5041 tgtctaatat cttttttgaa aaataatttt ccaattacga atccacttgg gaaagcgccg
     5101 ataagataac ttagtagtaa catgacgatt atcatcataa atattacaca tcctttaata
     5161 tcttaggact attttatcat attcatttaa attacggcta aaaattttaa aaacggggat
     5221 taatatatgg aattaagcta tgaaagttaa ttgatacttg cattttacgc tgatttatat
     5281 aagaataact attgtatagt tttaaaaacg aacgtacgtt tgcaggaggc gaaatcattg
     5341 gcaatgaata aacaaaataa ttattcagat gattcaatac aggttttaga ggggttagaa
     5401 gcagttcgta aaagacctgg tatgtatatt ggatcaactg ataaacgggg attacatcat
     5461 ctagtatatg aaattgtcga taactccgtc gatgaagtat tgaatggtta cggtaacgaa
     5521 atagatgtaa caattaataa agatggtagt atttctatag aagataatgg acgtggtatg
     5581 ccaacaggta tacataaatc aggtaaaccg acagtcgaag ttatctttac tgttttacat
     5641 gcaggaggta aatttggaca aggcggctat aaaacttcag gtggtcttca cggtgttggt
     5701 gcttcagttg taaatgcatt gagtgaatgg cttgaagttg aaatccatcg agatggtaat
     5761 atatatcatc aaagttttaa aaacggtggt tcgccatctt ctggtttagt gaaaaaaggt
     5821 aaaactaaga aaacaggtac caaagtaaca tttaaacctg atgacacaat ttttaaagca
     5881 tctacatcat ttaattttga tgttttaagt gaacgactac aagagtctgc gttcttattg
     5941 aaaaatttaa aaataacgct taatgattta cgcagtggta aagagcgtca agagcattac
     6001 cattatgaag aaggaatcaa agagtttgtt agttatgtca atgaaggaaa agaagttttg
     6061 catgacgtgg ctacattttc aggtgaagca aatggtatag aggtagacgt agctttccaa
     6121 tataatgatc aatattcaga aagtatttta agttttgtaa ataatgtacg tactaaagat
     6181 ggtggtacac atgaagttgg ttttaaaaca gcaatgacac gtgtatttaa tgattatgca
     6241 cgtcgtatta atgaacttaa aacaaaagat aaaaacttag atggtaatga tattcgtgaa
     6301 ggtttaacag ctgttgtgtc tgttcgtatt ccagaagaat tattgcaatt tgaaggacaa
     6361 acgaaatcta aattgggtac ttctgaagct agaagtgctg ttgattcagt tgttgcagac
     6421 aaattgccat tctatttaga agaaaaagga caattgtcta aatcacttgt gaaaaaagcg
     6481 attaaagcac aacaagcaag ggaagctgca cgtaaagctc gtgaagatgc tcgttcaggt
     6541 aagaaaaaca agcgtaaaga cactttgcta tctggtaaat taacacctgc acaaagtaaa
     6601 aacactgaaa aaaatgaatt gtatttagtc gaaggtgatt ctgcgggagg ttcagcaaaa
     6661 cttggacgag accgcaaatt ccaagcgata ttaccattac gtggtaaggt aattaataca
     6721 gagaaagcac gtctagaaga tatttttaaa aatgaagaaa ttaatacaat tatccacaca
     6781 atcggggcag gcgttggtac tgactttaaa attgaagata gtaattataa tcgtgtaatt
     6841 attatgactg atgctgatac tgatggtgcg catattcaag tgctattgtt aacattcttc
     6901 ttcaaatata tgaaaccgct tgttcaagca ggtcgtgtat ttattgcttt acctccactt
     6961 tataaattgg aaaaaggtaa aggcaaaaca aagcgagttg aatacgcttg gacagacgaa
     7021 gagcttaata aattgcaaaa agaacttggt aaaggcttca cgttacaacg ttacaaaggt
     7081 ttgggtgaaa tgaaccctga acaattatgg gaaacgacga tgaacccaga aacacgaact
     7141 ttaattcgtg tacaagttga agatgaagtg cgttcatcta aacgtgtaac aacattaatg
     7201 ggtgacaaag tacaacctag acgtgaatgg attgaaaagc atgttgagtt tggtatgcaa
     7261 gaggaccaaa gtattttaga taattctgaa gtacaagtgc ttgaaaatga tcaatttgat
     7321 gaggaggaaa tctagtgagt gaaataattc aagatttatc acttgaagat gttttaggtg
     7381 atcgctttgg aagatatagt aaatatatta ttcaagagcg tgcattgcca gatgttcgtg
     7441 atggtttaaa accagtacaa cgtcgtattt tatatgcaat gtattcaagt ggtaatacac
     7501 acgataaaaa tttccgtaaa agtgcgaaaa cagtcggtga tgttattggt caatatcatc
     7561 cacatggaga ctactcagtg tacgaagcaa tggtccgttt aagtcaagac tggaagttac
     7621 gacatgtctt aatagaaatg catggtaata atggtagtat cgataatgat ccgccagcgg
     7681 caatgcgtta cactgaagct aagttaagct tactagctga agagttatta cgtgatatta
     7741 ataaagagac agtttctttc attccaaact atgatgatac gacactcgaa ccaatggtat
     7801 tgccatcaag atttcctaac ttactagtga atggttctac aggtatatct gcaggttacg
     7861 cgacagatat accaccacat aatttagctg aagtgattca agcaacactt aaatatattg
     7921 ataatccgga tattacagtc aatcaattaa tgaaatatat taaaggtcct gattttccaa
     7981 ctggtggtat tattcaaggt attgatggta ttaaaaaagc ttatgaatca ggtaaaggta
     8041 gaattatagt tcgttctaaa gttgaagaag aaactttacg caatggacgt aaacagttaa
     8101 ttattactga aattccatat gaagtgaaca aaagtagctt agtaaaacgt atcgatgaat
     8161 tacgtgctga caaaaaagtc gatggtatcg ttgaagtacg tgatgaaact gatagaactg
     8221 gtttacgaat agcaattgaa ttgaaaaaag atgtgaacag tgaatcaatc aaaaattatc
     8281 tttataaaaa ctctgattta cagatttcat ataatttcaa catggtcgct attagtgatg
     8341 gtcgtccaaa attgatgggt attcgtcaaa ttatagatag ttatttgaat caccaaattg
     8401 aggttgttgc aaatagaacg aagtttgaat tagataatgc agaaaaacgt atgcatatcg
     8461 ttgaaggttt gattaaagcg ttgtcaattt tagataaagt aatcgaattg attcgtagct
     8521 ctaaaaacaa gcgtgacgct aaagaaaacc ttatcgaagt atacgagttc acagaagaac
     8581 aggctgaagc aattgtaatg ttacagttat atcgtttaac aaatactgac atagttgcgc
     8641 ttgaaggtga acataaagaa cttgaagcat taatcaaaca attacgtcat attcttgata
     8701 accatgatgc attattgaat gtcataaaag aagaattgaa tgaaattaaa aagaaattca
     8761 aatctgaacg actgtcttta attgaagcag aaattgaaga aattaaaatt gacaaagaag
     8821 ttatggtgcc tagtgaagaa gttattttaa gtatgacacg tcatggatat attaaacgta
     8881 cttctattcg tagctttaat gctagcggtg ttgaagatat tggtttaaaa gatggtgaca
     8941 gtttacttaa acatcaagaa gtaaatacgc aagataccgt actagtattt acaaataaag
     9001 gtcgttatct atttataccg gttcataaat tagcagatat tcgttggaaa gaattgggac
     9061 aacatgtatc acaaatagtt cctatcgaag aagatgaagt ggttattaat gtctttaatg
     9121 aaaaggactt taatacagat gcattttatg tttttgcgac tcaaaatggc atgattaaga
     9181 aaagtacagt gcctctattt aaaacaacgc gttttaataa acctttaatt gctactaaag
     9241 ttaaagaaaa tgatgatttg attagtgtta tgcgctttga aaaagatcaa ttaattaccg
     9301 tcattactaa taaaggtatg tcattaacgt ataatacaag tgaactatca gataccggat
     9361 taagggcagc tggtgttaaa tcaataaatc ttaaagctga agatttcgtt gttatgacag
     9421 aaggtgtttc tgaaaatgat actatattga tggccacaca acgcggctcg ttaaaacgta
     9481 ttagttttaa aatcttacaa gttgctaaaa gagcacaacg tggaataact ttattaaaag
     9541 aattaaagaa aaatccacat cgtattgtag ctgcacatgt agtgacaggt gaacatagtc
     9601 aatatacatt atattcaaaa tcaaatgaag aacatggttt aattaatgat attcataaat
     9661 ctgaacaata tacaaatggc tcattcattg tagatacaga tgattttggt gaagtaatag
     9721 acatgtatat tagctaaaaa ctatatgcaa tcacgaaatt aaatgataaa atacagtaat
     9781 gttaaatttt gactaaattc aagggattta tattaaatgc tgaccaagta cttatcgtta
     9841 aattagcgat atactttgac caaactaata taaatctctt gtattgtata agaaatgatg
     9901 atgtatctct atatttactg aaggttcaat tcggtctaat tttatagaag gtacatgatt
     9961 catgtagagg atgcgagggg tttattttga aagatttcga tagtttaata cctggatggt
    10021 ttaaagaatt tgtccatgtt ggtaccgatt taatatggtc tcaatattta attggtctat
    10081 tattgacagc tggattcttc tttacgatta gttctaaatt cgttcaatta cgaatgttac
    10141 ctgaaatgtt tagagcttta gtagaacgtc cagaaacttt agaagatggt aagaagggta
    10201 tttcgccatt ccaagcattt gcgattagtg ctggttcgag agttggtact ggtaatattg
    10261 ctggtgttgc gactgcgatt gttttaggcg gtccaggtgc agtattttgg atgtgggtta
    10321 ttgcatttat aggtgcagcg agtgcattta tagaagcgac tttggctcag gtttataaag
    10381 tacatgataa agatggtgga ttccgtggtg gtccagctta ctatattact aaaggtttaa
    10441 atcaaaaatg gctaggtatc gtatttgcga ttttaattac aattacattt gcatttgtat
    10501 ttaacacagt gcaatctaat acaattgcgg agtcgttaaa tacgcaatat aatattagtc
    10561 cagtaatcac aggtattatt ttagcaatcg taacagctat tattatattt ggtggtgtac
    10621 gtagtattgc tacgttatct tcgttaattg taccgattat ggctatcatt tacattggta
    10681 tggttttagt aatattgcta tttaatttag atcaaattgt tcctatgata ggtacgatta
    10741 ttaaaagtgc atttggtatc gaacaagtaa ctggtggcgc tgtaggtgct gcggttcttc
    10801 aaggtatcaa acgtggttta ttctctaacg aagctggtat gggttctgcg ccgaatgcag
    10861 cggcaactgc tgccgtacca caccctgtta agcaaggttt aatccaatca ttaggtgtgt
    10921 tctttgatac aatgttggtt tgtacagcaa ctgcaatcat gattttacta tattcaggac
    10981 tgaaatttgg tgataacgca cctcaaggtg ttgcagttac tcaatcagca cttaatgagc
    11041 atttaggttc tgctggaggt attttcttaa caatagcagt tacactgttt gcattttcat
    11101 ctgttgtagg taattactat tacggtcaat ctaatattga atttttatca acaaaccgtg
    11161 taatattatt tatctttaga tgtcttgttg tagtacttgt ctttgtcggt gcagttgtaa
    11221 aaacagaaac agtatggaat acggcagact tatttatggg cttaatggca attgtaaaca
    11281 ttatttccat tataggactg tccaatgtag cttttgcatt gatgaaagat tatcaaaagc
    11341 agaaaaaaga aggcaagaac cctgtcttta aacctgaaaa cttagaaatt aacttatttg
    11401 gaattagtgc ttggggcgct aacaaatata agaactctga taaataaata tttttaatgt
    11461 aacgattcat tatataaaaa tcaaataaac cctgaggttt tcaaattaaa gcgaaaatct
    11521 cagggtttta aatttgtact caattatcat ctgtatcatt acactagtta atctaagatt
    11581 tgattatata ttaatatagt aaggttgaga tttatttcct ccattggtgt gcaaagacta
    11641 taccattgat aagacatatt gttggataga tgaatataaa tttgttttag ctttgcacaa
    11701 tttaatagta cgttttagtt tgtattgagt attaattcag ataatatata aataaataga
    11761 attatttgta aatatgggag tcagattttt ttattatagt tattccactt tgagagacat
    11821 cgaaattaaa ttaagatata tcatatagga taggcgtgta atagaacaca agtatttaaa
    11881 ttattatact ttcaagttag acatttccga tatataatct taataataga aaaataggtg
    11941 atgataatgg gagaatatat tgttactaaa acattgaaca acaatgtcgt agtatgtact
    12001 aataatgatc aagaagttat tttaatcggt aaaggtattg gttttaacaa aaaagaggga
    12061 atggcgttaa acgaccaaac tattacaata gagaaaattt ataaattaga aagtgagcaa
    12121 caaaaagcac attataaaag tttagttgaa atcgctgatg ataatgtatt acaagtaatt
    12181 attgattcgt tgaattttat ttctaatact gcgatgaatg ttgattcaaa acaacttgta
    12241 gtttcattaa cggatcatat tatatttgct tataaacgct taaaacaaaa tcaagttatt
    12301 agcaatccat ttgttatgga aactatgcag ttatatagtg atgcatatca tattgctaaa
    12361 caggtgattg atcagttaaa tgcagcatta gatgtacatt ttcctgaaga tgagatagga
    12421 tttattgcat tacatattgc atctaataca gaagatttat ctatgcatga gatgaccttg
    12481 atcaataatg ttattaaaaa aggtatagat atcattgaat cagaccttgt gacaactgtt
    12541 gataaggaat cattacaata ccaacgtttt ataaggcacg tacaattttt aattcgccga
    12601 ttaagaagaa aagaatatat acatgca
//
LOCUS       DANRDI010000071        12582 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.071, whole genome shotgun sequence.
ACCESSION   DANRDI010000071 DANRDI010000000
VERSION     DANRDI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.071"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..370
                     /locus_tag="PZ377_001528"
     CDS             <1..370
                     /locus_tag="PZ377_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDM6358402.1"
                     /translation="DSILKAIKLIKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDI
                     YTGLLSNMASMVFLLGELNVSDLSDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDD
                     DMNRSLTYMLNMLKGMSREE"
     gene            598..831
                     /locus_tag="PZ377_001529"
     CDS             598..831
                     /locus_tag="PZ377_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358403.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            853..1629
                     /locus_tag="PZ377_001530"
     CDS             853..1629
                     /locus_tag="PZ377_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDM6358404.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            1940..3064
                     /locus_tag="PZ377_001531"
     CDS             1940..3064
                     /locus_tag="PZ377_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDM6358405.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(3156..4109)
                     /locus_tag="PZ377_001532"
     CDS             complement(3156..4109)
                     /locus_tag="PZ377_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDM6358406.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(4471..4971)
                     /locus_tag="PZ377_001533"
     CDS             complement(4471..4971)
                     /locus_tag="PZ377_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDM6358407.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(5208..5630)
                     /locus_tag="PZ377_001534"
     CDS             complement(5208..5630)
                     /locus_tag="PZ377_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDM6358408.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYIIPLTIRFI"
     gene            5878..6960
                     /locus_tag="PZ377_001535"
     CDS             5878..6960
                     /locus_tag="PZ377_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDM6358409.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            7055..8455
                     /gene="nhaC"
                     /locus_tag="PZ377_001536"
     CDS             7055..8455
                     /gene="nhaC"
                     /locus_tag="PZ377_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDM6358410.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            8629..8871
                     /locus_tag="PZ377_001537"
     CDS             8629..8871
                     /locus_tag="PZ377_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358411.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(8888..9691)
                     /locus_tag="PZ377_001538"
     CDS             complement(8888..9691)
                     /locus_tag="PZ377_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDM6358412.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            10301..12256
                     /locus_tag="PZ377_001539"
     CDS             10301..12256
                     /locus_tag="PZ377_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358413.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIM
                     HMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            12282..>12582
                     /locus_tag="PZ377_001540"
     CDS             12282..>12582
                     /locus_tag="PZ377_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDM6358414.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKM"
BASE COUNT         4397 a         1723 c         2251 g         4211 t
ORIGIN      
        1 agatagtata ttaaaagcaa tcaagcttat taaaacatta gatgatgcaa aactattaga
       61 tgctttaaat ggtgcaattc gaggacgtca agtcatcata aataaatttg cagttgaact
      121 taataaagat atttatacag ggctattatc taatatggct tcaatggtat ttttattagg
      181 tgaactcaat gtatcagatt taagcgactt cctaaataaa gttaataaag gattacacgt
      241 tgccaatcaa gcgagtccaa atgctaagac aactataaga agtctattgg gcgtattgaa
      301 agatgacgac atgaaccgta gtttaacata catgcttaat atgctaaaag gtatgtctcg
      361 agaagaataa cattacaatg aaaaagtgtt tctacatgca agcagtgtag gagcactttt
      421 tttaattttt gtaaaaatta aaaagggtgt ttttgatgga ataaatgaat aatattgaaa
      481 aacaaaaaaa tcacgtgcta ttgtgaatca atcatcgaca ggcctatgaa acatcatgta
      541 ttattatgat tatgtaagtt atttaaataa aaagggtgag tgcatattaa ttcgagaatg
      601 ttaaaaacgt cagctgtaat ttctaataca ttattagtca taggtttagt ctgtctattt
      661 atgatgaaat tagtgttagc tattactttt tttgctgttt cattatcaat tagtctagtt
      721 gtctttaata tgatgttcag agatagaaca acgatgaaag taatcgttaa cgcttctttt
      781 ttaatcgtaa tattagcgat tgtagtggca tattttttat tgtctaagta agaaaaatga
      841 ggttttgtct atatgaagaa gaatttcaag ttacgcattt caacgctact attgatagtt
      901 attttagttg tttttgctgt attactcatc gtgaatgaaa ctaaattgtt taaaaatgat
      961 gtgaattact cttttgatga ggctgtttca atgcaacaag ggaaaggtat tgtacagaca
     1021 aaagaagagg atggtaaatt tgttgaagca aataataatg aaattgctaa agcaatgact
     1081 atttcacata aagacaatga tatgaagtat atggatatca cagaaaaagt gccaatgtcg
     1141 gaatctgaag ttaaccaatt gctaaaaggt aaggggattt tagaaaatcg agggaaagtt
     1201 tttctagaag ctcaagaaaa atatgaggtt aatgtcattt atcttgttag ccatgcatta
     1261 gtagaaacag gtaacggcaa atcagaatta gcaaaaggca ttaaagatgg gaaaaaacgc
     1321 tattacaact tttttggtat aggagcattc gatagtagtg ctgttcgtag tgggaaaagt
     1381 tatgctgaaa aggaacaatg gacatcacca gataaggcga ttattggtgg tgcaaagttc
     1441 attcgtaatg aatattttga aaacaatcaa ctgaatttat atcaaatgcg atggaatcca
     1501 gaaaatcctg cgcaacatca atatgcgagt gacattcgct gggcagataa aattgccaaa
     1561 ttaatggata aatcctataa gcagtttggt ataaagaaag atgatattag acaaacatat
     1621 tataaataag acatcggtgc ttaaaggagc tggaacaatt tattgtttcg agctccttta
     1681 gcgcattctg agtgtgttag ttaaatggat tttaacctaa caaaaaacgc tatatagcat
     1741 caaatatgct atatcccaca tcattgttac aaatgtacat gatgtaaatg aatattgctg
     1801 tctaaatgtg catgtaatat acaatggtgc agataataca cttaagtcct taaaaatgaa
     1861 acgttagttc caagagtcat ttttaaacaa tagtgcatgt gataaaatag aaaagaatga
     1921 aaaatataga ggtgacaata tgaagatagc aattataggt gcaggcatcg gtggattaac
     1981 agctgctgca ttattacaag aacaaggtca tactattaaa gtctttgaaa aaaatgagtc
     2041 agttaaagaa attggcgctg ggattggtat cggagataat gtgcttaaaa aactaggtaa
     2101 tcatgactta gctaaaggta ttaaaaatgc tgggcaaatc ttatctacaa tgacagtgtt
     2161 agatgacaaa gatcgcctgt taactactgt taaattaaaa agtaatacat tgaatgtgac
     2221 gttaccacgc caaacattaa ttgacattat taaatcttat gtaaaagatg acgcaatatt
     2281 tacaaatcat gaagtcacgc atatagataa tgagacagat aaagttacca tacatttcgc
     2341 ggaacaagaa agtgaagcat ttgatttatg tattggtgct gatggaattc attctaaagt
     2401 gagacaatct gtaaatgctg acagtaaagt attatatcaa gggtatacat gctttagagg
     2461 tttaattgat gatattgatt taaagcatcc agattgtgca aaagaatact ggggaagaaa
     2521 aggaagagta ggtattgttc cgttattaaa taatcaagca tattggttca ttacaattaa
     2581 ctcgaaggaa aacaatcata aatatagttc gtttggtaaa cctcatttgc aagcatactt
     2641 taatcactat ccaaatgaag ttagagagat cttagacaaa caaagtgaaa caggtatctt
     2701 attgcataat atttatgatt tgaaaccact caaatctttt gtttatggtc gtactatttt
     2761 actaggagat gcagcacatg cgacaacgcc taatatgggg caaggtgctg gacaagcaat
     2821 ggaagatgct atcgtattag taaattgttt taacgcatac gactttgaaa aagcattaca
     2881 gcgttatgat aaaatacgtg tcaaacatac tgcaaaagta attaagcgtt ctagaaaaat
     2941 cggtaaaatt gcccaatatc gtagtcgttt atttgttgca gttagaaatc gtattatgaa
     3001 aatgatgcca aatgcattag cagctggaca aactaaattc ttatataaat cgaaagaaaa
     3061 ataatacaac aatatgaaaa cccccgtatg ttgaaacgag agctcaacat atgggggttc
     3121 ttgtttttat aatgttatta taataaattc aattattagt taacgacaaa ttgtggtttc
     3181 tcaccttgaa cggcactaat tgcagcatta gcaacaattt tagacatcat gtcacgtgct
     3241 tcaaatgtag cattaccaat atgcggtgtt aatactacat tattaagtga ttttaagtca
     3301 tcggtaatat ctggttcaaa ttcatataca tcaagtgcag caccttcaat ttcattatct
     3361 ttcaatgctt gcactagtgc ttgttcgtgc acgattggac cacgagaggc attgattaaa
     3421 tacgccgtag atttcatcat tttaaattgt tctgtatcaa ttaaatgatg cattttagga
     3481 ttataagcag cgttgatagt gataaaatct gcattcttta atagtgtatc taaatctaca
     3541 tattttgcac cgatttctcg ttctttttct tctttgcgat taggtccagt gtatagcaca
     3601 tccatgtcaa atgctcttgc acgacgagct actgcactac caatttcacc taaaccgata
     3661 atgccgattg ttttcccaga tacttctcta cctctgaaaa ataaaggtgc ccatccatca
     3721 aatccagttg tacgtgataa ttggtcccct tcaacaatac gacgcgctac tgcaagtact
     3781 aatccaattg ttaaatcagc agtcgcgttt gttgatgctt taggtgtgtt tgtaacatct
     3841 atactttttt ctcgggcata ctcgatatca atattattaa aaccagcgcc atagttggca
     3901 atgattttta agtctttacc agcatcgata acatctttat caacgtttgt agataataaa
     3961 ctaattaagg cagtcgcgtt tttaacacct ttaattaaag tgtctttatc gactaatcct
     4021 ttaccttcat acatttcaac ttcaaaatgt tcttgtaaaa gttttaaacc tacttctggt
     4081 attgcaccag caacataaac tttttccata aaagatcact ccttttatct tagtatagta
     4141 gaagattaga cagtatacaa ctatgtcatg atgtcttgtg tatcaatgat gtaagcgcgt
     4201 acttttgatg gaggtgatat aacttaggca ctgtagaact atgaatattg taatgtggaa
     4261 aaactggatc aattaaatta gataacgtag ttttaaagtt aatagtatta gaaaaaatta
     4321 atattttgaa tatgggagga aatataaata agtaggtggc aacgaaaaat agcaaaaaaa
     4381 gagcttctcc tataaaggaa agctcaaagt tttttgatga catatgtact agaattaagt
     4441 ttcaagacaa tatgtatcat cgtgtttata ttaaatatgg atgtagttgt agttacctgc
     4501 ttcacttgca gaaatagttc tagaacttac tgagaaaggt ccgccactat aattcatttc
     4561 tgaaattgta actgaaccat cactgtttac actttctaca tatgcaacgt gaccaaatgg
     4621 tccttcagaa gattgtagga tagcgccttt agaaggtgta tgatttactg tgaatccagc
     4681 accttgtgca gcagcagccc aattattagc atttccccaa gtagaaccga tttctccgcc
     4741 aactttatca tatacatacc aagtacattg tcctgcagtg tataagttac cagaatgtga
     4801 aattgatgat gtagttgtcg tagttgtcgt agtcgttgta gtttgagtcg tgttgtagtt
     4861 atagttgttg taatttgtat aattttcagc agcatctgca tgatgtgctt gacctactaa
     4921 tgctgtgccg attcctgctg ttaacgtagt tgctgttact aattttttca tgaataaagt
     4981 cctccaaagt tctatatctt tttttataaa taaaacgtag cgactgtttt attctcacat
     5041 ctcgaattga tgacaatagt tactttaaca aaattaatgc ttcttgtggg gaatgttatt
     5101 gatttgtaaa agaataaaaa aactttgact aattttgtaa taaaaattag tcaaagttac
     5161 aatgagatta acagataatt aataggaaat atttatttgt aatatgttta aataaatcga
     5221 attgttaaag gtattatata ttcttggcca ttataatatt tgacacacgc aataattgtg
     5281 aatacaaaag ataatattga gaaagcgaat atggataaaa taccgataaa cgtaatgatg
     5341 aaacctataa taataatgaa atcaatatct gtagcaatta ggaaaacgcc tattaaagtg
     5401 ataacgacta aaacgataga ccaaataata taagaaatcg tatagttaag ataatttttt
     5461 ccagcacgat caactagttt cgattcatct tttttcaata accatattat cagtggacca
     5521 ataatagatg tgaataaact taataaatag ataagcatcg ccataatgtt ctcatcattg
     5581 gatttgcgat tcggttgatg atttgttacg tcgttcattt cagttgtcat attagacact
     5641 cctttgaaaa ttgtaatatt atctttaact ataacaaaat ataatcaaaa ataaacatgt
     5701 ttattaaaca attattaaaa ataaaaataa ttggtggacg tcggcgttta aataggttaa
     5761 tttaaggtta tatatactta acatttataa tgatgcgtaa tgaattcgca tcatttttat
     5821 attgtcttac gtataatttg tttttaattt taaccaaaga tagaaagagg gttgtttatg
     5881 aaaatagcaa ttgtaggatc aggaaatggc gcagttacgg cagcagtaga tatggtgagc
     5941 aaaggccacg atgttaaatt atattgtcgt aatcaatcta taagtaagtt tcaaaacgca
     6001 atcgaaaagg gcggatttga ttttaataat gaaggtgatg aacgtttcgt aaaattcact
     6061 gatattagtg atgatatgga atatgtttta aaagatgctg aaattgttca agtgattatt
     6121 ccatcttcat acatagagta ttatgctgat gtaatggcag agcatgtaac tgataatcag
     6181 ttgatattct tcaacatggc tgcagcaatg gggtcaattc gttttatgaa tgttttagaa
     6241 gatagacata ttgaaacaaa accacaacta gcggaagcta atacgttgac gtatggtacg
     6301 cgtgtcgatt ttgaaaatgc agcagttgat ttatctctaa atgtacgtcg tatcttcttt
     6361 tcaacatatg atagaagctg tctaaatgat tgttatgaca aagtttcaag tatttatgat
     6421 catttagtaa aagaggaaag cttaattaaa acaaatcttg aaaatggtaa tcctgaagtg
     6481 catccaggac caacattatt gaatgtcggt cgcattgatt atgctggcga gttcgcttta
     6541 tataaagaag gaattactaa acatacagtt agattacttc atgcaatcga attagaacgt
     6601 ttgaatttag gccgtagatt aggttttgaa ttatcaacag ctaaagaatc acgtattgaa
     6661 cgtggttatt tagaacgtga taaagaagat gaaccattaa atcgtttgtt taatacaagc
     6721 ccagtatttt cacaaattcc aggaccaaat catgtagaaa gcagatattt aactgaagat
     6781 attgcatatg gtttagtact atggtcaagc ttaggtcgtg ttattgatgt accgacacca
     6841 aatatagatg cagtaattgt aattgcatca accattttag agagagactt ctttgaggaa
     6901 ggcttaacag ttgaagaaat tggtttagat aagcttgatt tagaaaaata tttaaaataa
     6961 atgatggctt gaagatagaa aaggatatag cattatgcaa aagcaataaa ttgaagaaaa
     7021 gaggtttctc atcaataagc gaaggggacg atagatgatg aaaagaaaac ccaccttttt
     7081 agaatcaatt tcgacaatga ttgtaatggt tattgttgtt gtaacaggct ttgtgttttt
     7141 tgatattcca attcaagtat tattaattat tgcctcagca tatgccacat ggattgcaaa
     7201 acgtgtaggc ttaacatggc aagatttaga aaaaggcatt gcagaacgtt taaatactgc
     7261 aatgcctgca attttaatta tactagcggt aggaattata gtaggcagtt ggatgttttc
     7321 tggcacagtg ccagccttga tttattatgg cttagattta ttgaatccaa gctatttttt
     7381 aatatcagcc ttttttataa gtgctgttac atctgtagca actggtacag catggggctc
     7441 tgcatcaact gcagggattg cacttatttc tattggtaat caattgggga ttcctccagg
     7501 gatggcagcg ggtgctatta tagcaggggc tgtgtttggc gataaaatgt caccattatc
     7561 agatacaact aatttagcgg cgcttgttac taaagttaat atatttaaac atatacattc
     7621 gatgatgtgg acgacgatac ctgcatcaat cataggttta ttagtatggt ttattgctgg
     7681 atttcaattt aaagggcatt caaatgataa acagattcaa actttgttat cagagcttgc
     7741 acagatttat caaattaaca tatgggtctg ggttccctta attgtgatca ttgtttgttt
     7801 gctatttaaa atggctacag tgccagctat gctaatatca agcttttctg ccattatagt
     7861 ggggactttt aatcatcatt tcaaaatgac agatggtttc aaagcaacat ttagtggttt
     7921 taacgaatca atgatacatc agtctcatat ttcatccagt gtgaaaagct tgttagaaca
     7981 gggtggtatg atgagtatga cccaaatatt agtaacgata ttttgcggat atgcatttgc
     8041 aggtattgta gaaaaagcag gatgtttaga agtcttatta actactattt ctaaaggcat
     8101 ccattctgta ggaagtttaa tatgtattac tgttatttgt tgtattgcgc ttgtattcgc
     8161 tgcaggtgtt gcttcgattg taattattat ggtcggtgtg ttaatgaaag atttgttcga
     8221 aaaataccaa gtctctcgtt ccgttttatc aagaacactt gaagactcaa gtacgatggt
     8281 cttaccttta ataccatggg gaacatcagg tatttactat acgaatcaac ttcatgtctc
     8341 tgttgaagaa tttttcatat ggacagtacc atgttattta tgcgcaatta tagcaattat
     8401 ctatggtttt acagggatag gtattaaaaa gtcatcgaat tcacgtttaa cttaatgtga
     8461 gcgtggaata tatataatat gttgaaacac tttaatcatt tataattgta gcggttataa
     8521 tttgaaaagg ttttaactta gaataaatat cctctataca tatactgaat atgttttgta
     8581 gcggaacatg ttgatatatg taatgtaagt tttatgtcat gatttgtaat gactaaatta
     8641 attgagaatt tgaaggcaag tatatttgta agtactttaa ctaaaaattt atcaatgtat
     8701 agccgatttg acatgcctaa atttgggtgt gtcaatggct gtatgttgtt tattctttat
     8761 tacagagtga atcggattgg tgaaaatcga aattttgaga tttttaccaa ttcgattttt
     8821 ttcatagaaa ttaaaaaagc caacaaggct cttgaaacct tgttggcgta aacatagcca
     8881 tcactaatta gtgaatgaag ttataaccag cagcttggct agctgagatt gtacgtgaag
     8941 ttacaacacc tgggccataa ccatagttca tttctgaaac tcttactgaa ccattgctgt
     9001 taacactttc aacgtatgca acgtgaccgt atgcaccttg agttgtttgc ataattgcac
     9061 cagcttttgg tgtattgttc actgtgtaac cagctcttgc agctgcgtta gcccagttac
     9121 ttgcattgcc ccaagttgaa ccgattttac cacctacacg atcaaatacg tagtatgtac
     9181 attgaccaga agtgtataag ttacgtcctg aagtataacc acttgagatt gaacggccat
     9241 ttgatgatgg agccatagtt gtagttactt gaacattgtt gcttgaagtg ctgtagcttg
     9301 cacctaaacc accagtacgg tagctgtttg tgttgtaact attatagtta ttgtagttat
     9361 atgattgatt attatttgag tagttgttgt aacggctgta gttattgtag ctataaccgt
     9421 tgttgtaatt gttatagtta ttgtaaccat tgtagtagta atagctgtag tagccattat
     9481 cttggtttaa ttgacttgga tgccagttac ctttccatgt gtaatggtag ttaccttgtg
     9541 catcaatagt gtaagtatag ctatatgatg ttgggtcgtt tggattataa ccgtagttat
     9601 cttgctcaga agcatgagct tgatttcctg atgcaattgc gattgtagcg aatcctgcag
     9661 ttgcgatagt agctgtagcg attttcttca ttttaaaaat atcctcctaa aaattttaaa
     9721 tctaaaatat tttcgtaatg tccgtgtgac aaaattaatg ttataagtta tctctcgtaa
     9781 ttaaacgaca agaaagacta taacagaaat tagcgtcctt gtgtgctttg ttaacgtttt
     9841 gtaatttttt gctaatatct tgacacaata gaattttaaa agtatagaaa tttgcatttt
     9901 gcaaaactta taactacggc attctttgtg aaaactgaat gtttcgaaaa taagtctgtt
     9961 acaaatttgt aatattactg aaaattctaa atgtatattt tgtgcataat ataggacttt
    10021 taatcagaaa atgcataaaa attcgacacg aacgatcaag tttctatgat tatgaaagct
    10081 ttggtatttg gatgaaaatg tatatttaat ggataaaata tcctaattta gcataaaaaa
    10141 atgttttaat aaaagtatta tttgatataa tcgatttatg ttttgttact gctaaaaaac
    10201 atgtggcgat ataattggta gtgaatatta gatgttgaca attgatttga gaaagacaaa
    10261 caggagtaaa tcaatatatt tgtatatgtg aggatgtaag atgacagata attattcact
    10321 acatatttat aaaagtatga aaatgagtag tgtgaaggaa gcgaacttaa aaattatata
    10381 ttggttacaa ggtacaggtg tagtttcaat caatctacaa caatatgatc taaaaagaaa
    10441 ttatgtcacc attattatgt tgaatgattt gtatcaaatt gatggtaatg atgatagtgt
    10501 atgttgtgtt gttgaagtaa gtgcaaagac atttttgaag tttatgaatg caaactatat
    10561 gatgcgtggc aagatattaa caaatgaggt tgctagcact tttagaatct tgattaagta
    10621 tttaatctac agcaaaatta aacaacagtc ttataatgct aatgataaga ttattaatta
    10681 catgtgtgtt gaattaatgt cattaaatac atacgatggt agtcgtcatt tagtagccga
    10741 agaagtgcac gattatttaa caaacaacca ccataaaaag ataaatagga aagatgtcat
    10801 taatcaagtt agtattacaa acaaagcttt aagtgacatg tttaaagcga caccttatag
    10861 taattttatt caatatttaa atcatattag actggagcat tgcttaattg atattttaac
    10921 ttctaagaag cctattgaag aaatcgcaag tagtcatggt tttaatcatt attctcgttt
    10981 tatacacctc tttaaagaaa cgtatggtaa cactcctaaa ttaattagaa aaagatatag
    11041 tccaacatct cattcaatta accattccca attagttgaa attgataaaa atataattga
    11101 attatttgat gatactaatc aaagttcttc aagtatgcgc gagattgata taccttttga
    11161 gcctacaaaa aggagtcaaa tgtatcaacc taaaaacatt tatattaaag gaagtaattt
    11221 ttattggatg gattattata ctattaaaca aatcattcaa agattaggtg ttaatcctga
    11281 aaacttgcat ataattgtta caatcgacac aagcaatgag ctatcgattg aacaaatcac
    11341 tttattactt caaaagatag tgaattacaa tgtaaatgta gtgtttcgta ttaaacatga
    11401 atataaggaa ttgttaacga gtgctgaaag aggcaagata gaaaagctag tagctacaat
    11461 ttttaatatg acttttgaga gtaatagatg taagattgca tttttaatta acgacttaaa
    11521 tactactgca attcaaaaac tgaaacgttt aatagataat tatataggag tatttgaatt
    11581 aatatatcat ctggaaccaa aagagttgaa tacgcgctat gataaagaaa ttgatcatct
    11641 aatagactat tttatcttac cgctaagaca aatagaacag gtgtctattg cacgttctaa
    11701 aattatatgt gatgtagatt tgattaatga gaataatgac ttaaaagaaa aaatcacaat
    11761 gtctgatatt aataaggttt ctgaactttt gacaaattat acaaaatttc gaggacttat
    11821 tatgcacatg tcaattataa gtgaatcgag caaagaaata aatttaacta gattatcaat
    11881 attcattttt atcataaata tacttaatca attacgaggg attgttattt atcaaaatga
    11941 cacgatgatt gtaacgaagt tcaaacatga agtacaatgt gtcatttgtt tgccagccgc
    12001 attgttaaat gagaattcag acgctatacg tttatcgtgt aagggtcttc aacaatttag
    12061 ccatgctaaa actaaacaaa ttgttttaag tatagataaa gataatcata cttccgaaaa
    12121 tggtgtactt gtgaatgata ttatgcaaca acacagttat tggaaagcta attttataga
    12181 aagaaatatt tatgatcatt cattaatggt ctcaagcaat agtattgttc atttgaaata
    12241 tcatcttgag atataggcat ggtttaagag aggttttaaa gatgtctaaa tttaaagtta
    12301 agcgtttaac tgcacacata ctaatgttga aagtcattaa tgatatgttg aaatattcat
    12361 ttcatctcac tttgactcaa gtgaaactat taaatctatt agttaaatat gataattctg
    12421 attccaatag cgcttcaata gatagtttat taaaaatgaa agaaatccaa tctaagatgg
    12481 cccttcttca aatgttaagt tatttaaaca atcatcaatg gctattgaaa ggaagagatc
    12541 aaagggatca acgtaaattg actatttcga ttaataaaat gc
//
LOCUS       DANRDI010000072        12377 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.072, whole genome shotgun sequence.
ACCESSION   DANRDI010000072 DANRDI010000000
VERSION     DANRDI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.072"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(707..1354)
                     /locus_tag="PZ377_001541"
     CDS             complement(707..1354)
                     /locus_tag="PZ377_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDM6358415.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(1382..2866)
                     /gene="cls"
                     /locus_tag="PZ377_001542"
     CDS             complement(1382..2866)
                     /gene="cls"
                     /locus_tag="PZ377_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDM6358416.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            3032..3325
                     /gene="csoR"
                     /locus_tag="PZ377_001543"
     CDS             3032..3325
                     /gene="csoR"
                     /locus_tag="PZ377_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDM6358417.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            3337..3546
                     /locus_tag="PZ377_001544"
     CDS             3337..3546
                     /locus_tag="PZ377_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDM6358418.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            3674..3811
                     /locus_tag="PZ377_001545"
     CDS             3674..3811
                     /locus_tag="PZ377_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358419.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(4075..5277)
                     /locus_tag="PZ377_001546"
     CDS             complement(4075..5277)
                     /locus_tag="PZ377_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDM6358420.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            5595..6665
                     /locus_tag="PZ377_001547"
     CDS             5595..6665
                     /locus_tag="PZ377_001547"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDM6358421.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            6680..8038
                     /locus_tag="PZ377_001548"
     CDS             6680..8038
                     /locus_tag="PZ377_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDM6358422.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            8555..10075
                     /locus_tag="PZ377_001549"
     CDS             8555..10075
                     /locus_tag="PZ377_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDM6358423.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(10444..11139)
                     /locus_tag="PZ377_001550"
     CDS             complement(10444..11139)
                     /locus_tag="PZ377_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6358424.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(11139..>12377)
                     /locus_tag="PZ377_001551"
     CDS             complement(11139..>12377)
                     /locus_tag="PZ377_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDM6358425.1"
                     /translation="ILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFNDSIKQTYTV
                     ENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQALSWVIKNER
                     QAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESMLNELSLAVE
                     NVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSHSKDMSIIEK
                     EQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEIDMILERRHLK
                     KRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYASYEQIEFAV
                     IDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKHQSNLQYKPN
                     QPHGSIFYFNIYTDFNEGDV"
BASE COUNT         4392 a         1889 c         2146 g         3950 t
ORIGIN      
        1 acaatatgtg ataaatagaa acagcagtaa gatgatttca aatgtgaaag tcatcctact
       61 gctgttttta tttgaaataa tattaacgag ttttcagtat gtaaaaagtg gttaataaca
      121 gagttgtcat tcttcatcgc aatgtcatct tgacacaatg attcatcagt gtagcagtat
      181 atgtcgcaaa tttccacagc aattcactta tacatgtaac agtgtgaaat aataacgtca
      241 caacggcata atacacattg caacgtccca acaacatatc atagaatcgt aacggcatga
      301 catcaacacc aaacctggct ataacaccat aacagcgcaa cgccacatca tagcactaca
      361 tcaagtcgca tcgtagcacc agcacatcaa agtaacatgg catcaacatc aaaccggtct
      421 ataacaccat aacaccgcaa tgccacatca tagcactaca tcaagtcgca tcgtggtacc
      481 agcacatcaa agtaacatgg catcaacgcc aaacctgtct ataacaccat aacaccgcaa
      541 tgccacatca tagcactaca tcaagacgca tcgtggtacc agcacatcaa agtagcatga
      601 catgtcacca agctcccaca acaacacatc aaagtaacat ggcatgacac caagcttcca
      661 caacaacacg ccattacatc gcatcatcat actacaactt caatgtctag tcgtgacaat
      721 tccattccgt aaaaaactgt ttcaagtaca tcatcataaa gtcgtgacgt tctttagcaa
      781 tcatcttcgc cgtatctgta tgcattaaag attctaactt aagtaatttt tcaaagaaat
      841 gtttaattgc agatggtggc aactgttcaa ctaaatcatc attaatctta tctagtgaca
      901 tatgttctgt ccacataggt tcaccaaagt gtcctgcaaa ttgaaatgtt cgtgcaacac
      961 ctatagcgcc tatagcatca agacgatctg catccctgac aatttgacct tctaaagata
     1021 aagtgacatg atcatttttg ccattgcgat agctcatatt attaataata aataaaatgt
     1081 gctcttgatc ttcggttgat agtgataaag aagataaaaa tgacttcaat tcaacatatt
     1141 gtttgttagc atctacaact ttttcgtcaa cggtatcatg aagcaaacat gcgagttcaa
     1201 tgactaaagt atcattaaca ccttcatttt cagcgattga tttagctaaa gcagtgacac
     1261 gatatacatg cgcaatatca tggcctgtat aatcattttg atgaatagat tccatatatt
     1321 ttttggcagc actaatgacg tctgattgtt gcattaaagt tacactcctc atatttctat
     1381 tttataagat aggtgacaat aattgtgaaa taccttcttt aaatttaatc caaagacttc
     1441 gcttagcata acgtgctttt gttaattcag aagatactgc taaatcatct ataaaagctt
     1501 gttttaattt tttggcaatt tgttggtcat aaataaaagc gttgacttcg aaattcaatg
     1561 tgaaactgcg atggtccata ttagctgttc ccacacttgc aatttcatca tctataacaa
     1621 gtgtttttga gtgtaaaaag ccattgtcgt agtgaaatac tttaacaccg gcatctaata
     1681 aggatgctgc attttttaaa gtagcccaaa aaacaaacgg atggtcaggt ttattaggaa
     1741 tcatgatatt gacatcaaca ccacctaatg ccgcaatttt aatagaatct aaaaaggctt
     1801 gatcaggtat gaaataggga gattgaatat aaatcgattt tttagcagat gaaatcattt
     1861 tcaaatagcc gtatttaatc tgttcccatt cttcgtcagg accactagaa gctatttgaa
     1921 cgccaattgt tccaccagaa tttacatctg ggaaataacg atcatcatag gagatgtggt
     1981 cacgtgtggc ttgtgaattc caatctagaa taaatcgtaa ttgcaatgca ttcactgcat
     2041 ccccgacaat tcgtaaatgc gtatctcgcc aatagccgaa tttttttgat ttacctaagt
     2101 actcatcacc aacattaaaa ccaccaacat atccaatttg cccatctatt acaacaattt
     2161 ttcgatggtt tcgattgttc atacgcaagt taattaaagg taattttgat gggaaaaatg
     2221 cttcagcatg tccaccttta ttgcgaaacg ggcgtaagcc ttttttacgc agtccacgag
     2281 aacccatgtc atcataaaga attttaactt ctacaccttg ttccgctttt ttaccaagtt
     2341 catttaaaat ggtacgacct aattcatcat tttgaataat atagtactga aaatgaatat
     2401 aatcagtagc attacggatg tcttgtatta ggtcatcaaa tttttcttgg ccgtctgtgt
     2461 atatttttaa atcgttgtct gttgttaaaa atgctgcgtt attatataac aacatttgaa
     2521 tcatttcttt aaattttaca atttgataat tggaatttga aaagttttca ttttttaaag
     2581 cagctaattg ctcatcaacg attaactcta atcctttttt atcttcctta tcaattttga
     2641 aaatttggtc acgttgaatt tgtcgtccta atagtaagta taaaatgaag ccgaataaag
     2701 gcaagaaaac taagactagt aaccaagccc agatagaatt ggcagaacgt ctttccatga
     2761 aaataatggt aaaggcgaat aataagttta aaataaatgc accaataaat attgaattta
     2821 aaataatatt agaatgcttg agtgcaatgg ataataactc tatcatttga aacctcccat
     2881 cgaaaatctt aaacgagtag tatatcttat taattgtatc atgaagcaat ttaaatatca
     2941 tgacaattta gtttgaaaaa cacactttgt gttgacgttg tgttttatgg gatatagtat
     3001 agtaaggaat gtaaatgaag gagtgaatgc tatgactgaa caagataatg cacatcattc
     3061 tgaacaaata aaaacgaatc ttaaatcacg tttaaatcga attgaaggac aagtgagagc
     3121 gattaatcgc atgattgaag aagatgtcta ttgtgatgat gtccttacgc aaataagagc
     3181 gacacgttcg gcgttaaaca gtgttgcgat aaagttatta gaacaacata tgaaaagttg
     3241 tattatgaat aaagttaatc aaggtgctca ggaagaggca atggaagagt tattagtgac
     3301 ttttcaaaaa ttgattaaag actaaaggag tttaagatga tacatcaaaa tacgatttac
     3361 acagcgggaa ttgaaacaga agaacaagta agtcaattga cagaacgcat ttcaaatatg
     3421 ataggtgttc atcaagtgaa tattaatata atagatggtc aagtaactgt atcgtatgag
     3481 acaccagcaa atttgaatag tattgaaaaa gaaatctatg atgaaggata caaaattgta
     3541 ttttagggta taatgtagag tgcgctatag attttaattt tgaaaataaa ttaaaaattt
     3601 tgtaaatgat gtagtaaagg tatgtcgaat agggtaagat aatattgaaa gcgatccaaa
     3661 cgaggtgata ataatgaaac gtcctgaaaa gattcaaaat gtagtcaaac tattgtcatc
     3721 attaggtgtg aatattaaaa aaactaaatc tcgtttagac attattaata ctttgccagc
     3781 atctaataaa gtaagtcacg aattaaaata attgactgtg attgtgaatc acacaccgtt
     3841 aaagctatcg tcggactatt cgatgatagc ttttttgact ggatttacta aaatgaaagc
     3901 aagtactata taacaaaatc cctctttaga gtgtacattt tacaatgtgt acttcaaaga
     3961 gggattgtta ttatcgagta atatctatat atgacatttt aaatcgacga cgtaaataga
     4021 ctatataacg tcatcttgaa aagttatgat atttgaaact caaaatagtt taaattaatt
     4081 actttttgga tggtataaat cgacatatcg ttttggttca tgataataaa ttgataagac
     4141 tatacctatt ccagtcatca tactccatag cgcactacca ccataactaa taaatggtaa
     4201 tggaatacca gtgattggta acaactgaat tgtcatacca atattttgta aaatatggaa
     4261 cacaagtaaa gtgacgaaac caacgataaa gattttgtta aattgatctt caattttcgc
     4321 agctaatctt attagatgga agattaaaaa taaaaatatt aagatcaata tgacagaacc
     4381 gataaagcca agttcctctc caatcactga aaagataaag tcagtatgat tttcaggtat
     4441 ataaacttca ccgtgattgt atcctttacc tagtaactgt ccagaaccga tagctttaag
     4501 tgattcagtt aaatgatagc catcaccact actatatgta taggggtcaa gccatgaatt
     4561 gattcgtccc atttgataca gttggacacc taataaattt tcaattaatg cgggtgcata
     4621 tagaatacct aaaatgactg tcattgcacc aacaatacct gtaataaaga taggtgctaa
     4681 gatacgccat gttataccac ttactaacat cacacctgca ataatagcag ctaatactaa
     4741 tgtagttcct aggtcatttt gcagtaatat taaaatactt ggtactaacg agacaccaat
     4801 aattttgaaa aataataaca aatcactttg gaatgattta ttgaatgtga attgattatg
     4861 tctagaaacg acacgcgcta atgctaaaat taaaataatt ttcatgaatt cagatggctg
     4921 aatactgata gggccaaacg tgtaccaact tttggcacca ttgataatag gtgtaatagg
     4981 tgactcagga ataacgagca agcctattaa taatagacag attaagaaat acaataaata
     5041 tgtataatgt ttaatctttt taggtgaaat aaacatgatg atacctgcaa aaattgcacc
     5101 taaaatgtaa taaaaaattt gtctgatacc gaaattagca ctgtattgac caccgcccat
     5161 tgccgagtta ataagcagaa cactgaaaat tgctaaaaca gctatagtgg ctactaatac
     5221 ccagtctact ttgcgaagcc aatgcttatc cggctgttga cgagatgaat aattcattgc
     5281 aaactccttt tatactcact aatgtttata tcaattttac atgacttttt aaaaattagc
     5341 tagaatatca cagtgatatc agctatagat ttcaatttga attaggaata aaatagaagg
     5401 gaatattgtt ctgattataa atgaatcaac atagatacag acacataagt cctcgttttt
     5461 aaaatgcaaa atagcattaa aatgtgatac tattaagatt caaagatgcg aataaatcaa
     5521 ttaacaatag gactaaatca atattaattt atattaaggt agcaaaccct gatatatcat
     5581 tggaggaaaa cgaaatgaca aaagaaaata tttgtatcgt ttttggaggg aaaagtgcag
     5641 aacacgaagt atcgattctg acagcacaaa atgtattaaa tgcaatagat aaagacaaat
     5701 atcatgttga tatcatttat attaccaatg atggtgattg gagaaagcaa aataatatta
     5761 cagctgaaat taaatctact gatgagcttc atttagaaaa tggagaggcg cttgagattt
     5821 cacagctatt gaaagaaagt agttcaggac aaccatacga tgcagtattc ccattattac
     5881 atggtcctaa tggtgaagat ggcacgattc aagggctttt tgaagttttg gatgtaccat
     5941 atgtaggaaa tggtgtattg tcagctgcaa gttctatgga caaacttgta atgaaacaat
     6001 tatttgaaca tcgagggtta ccacagttac cttatattag tttcttacgt tctgaatatg
     6061 aaaaatatga acataacatt ttaaaattag taaatgataa attaaattac ccagtctttg
     6121 ttaaacctgc taacttaggg tcaagtgtag gtatcagtaa atgtaataat gaagcggaac
     6181 ttaaagaagg tattaaagaa gcattccaat ttgaccgtaa gcttgttata gaacaaggcg
     6241 ttaacgcacg tgaaattgaa gtagcagttt taggaaatga ctatcctgaa gcgacatggc
     6301 caggtgaagt cgtaaaagat gtcgcgtttt acgattacaa atcaaaatat aaagatggta
     6361 aggttcaatt acaaattcca gctgacttag acgaagatgt tcaattaacg cttagaaata
     6421 tggcattaga ggcattcaaa gcgacagatt gttctggttt agtccgtgct gatttctttg
     6481 taacagaaga caaccaaata tatattaatg aaacaaatgc aatgcctgga tttacggctt
     6541 tcagtatgta tccaaagtta tgggaaaata tgggcttatc ttatccagaa ttgattacaa
     6601 aacttatcga gcttgctaaa gaacgtcacc aggataaaca gaaaaataaa tacaaaattg
     6661 actaactgag gttgttatta tgattaatgt tacattaaag caaattcaat catggattcc
     6721 ttgtgaaatt gaagatcaat ttttaaatca agagataaat ggagtcacaa ttgattcacg
     6781 agcaatttct aaaaatatgt tatttatacc atttaaaggt gaaaatgttg acggtcatcg
     6841 ctttgtctct aaagcattac aagatggtgc tggggctgct ttttatcaaa gagggacacc
     6901 tatagatgaa aatgtaagcg ggcctattat atgggttgaa gacacattaa cggcattaca
     6961 acaattggca caagcttact tgagacatgt aaaccctaaa gtaattgccg tcacagggtc
     7021 taatggtaaa acaacgacta aagatatgat tgaaagtgta ttgcataccg aatttaaagt
     7081 taagaaaacg caaggtaatt acaataatga aattggttta cctttaacta ttttggaatt
     7141 agataatgat actgaaatat caatattgga gatggggatg tcaggtttcc atgaaattga
     7201 atttctgtca aacctcgctc aaccagatat tgcagttata actaatattg gtgagtcaca
     7261 tatgcaagat ttaggttcgc gcgaggggat tgctaaagct aaatctgaaa ttacaatagg
     7321 tctaaaagat aatggtacgt ttatatatga tggcgatgaa ccattattga aaccacatgt
     7381 taaagaagtt gaaaatgcaa aatgtattag tattggtgtt gctactgata atgcattagt
     7441 ttgttctgtt gatgatagag atactacagg tatttcattt acgattaata ataaagaaca
     7501 ttacgatctg ccaatattag gaaagcataa tatgaaaaat gcgacgattg ccattgcggt
     7561 tggtcatgaa ttaggtttga tatataacac aatctatcaa aatttaaaaa atgtcagctt
     7621 aactggtatg cgtatggaac aacatacatt agaaaatgat attactgtga taaatgatgc
     7681 ctataatgca agtcctacaa gtatgagagc agctattgat acactgagta ctttgacagg
     7741 gcgtcgcatt ctaattttag gagatgtttt agaattaggt gaaaatagca aagaaatgca
     7801 tatcggtgta ggtaattatt tagaagaaaa gcatatagat gtgttgtata cgtttggtaa
     7861 tgaagcgaag tatatttatg attcgggcca gcaacatgtc gaaaaagcac aacacttcaa
     7921 ttctaaagac gatatgatag aagttttaat aaacgattta aaagcgcatg accgtgtatt
     7981 agttaaagga tcacgtggta tgaaattaga agaagtggta aatgctttaa tttcatagag
     8041 attagtcgag ggacctttta cttataaaaa tgatttgaat taatactaaa agattacaaa
     8101 gaagaggtgg ttttgtgtgt aaatacaaaa ttgccttttt ctttttatgt taaatctata
     8161 aatttgaaac taaatcaagg ttaattctat gtacacactt tatataggaa gtagtttgaa
     8221 tgtttatata atgttttaca aaaagatgta gtattataat gtctaatttc acatgtgttt
     8281 cagtaaaatt tgttgtggaa tgttaacgat atacgtattt tataaaaaat tttttataat
     8341 gattattcga atgatgcgta acgcttacat cttatctaat gctagctttt tgacaaaaat
     8401 atgacaatca attaatgtga ttctaataaa tattcgcaaa ttgctttatt gcgattaaat
     8461 ttttttggtg gtactatata gaagttgatg aaatattaat gaacttatat gcaaaagtat
     8521 attgagaaat aaacaggtaa aaaggagaat tattttgcaa aattttaaag aactagggat
     8581 ttcggataat acggttcagt cacttgaatc aatgggattt aaagagccga cacctatcca
     8641 aaaagacagt atcccttatg cgttacaagg aattgatatc cttgggcaag ctcaaaccgg
     8701 tacaggtaaa acaggagcat tcggtattcc tttaattgag aaagtagtag ggaaacaagg
     8761 ggttcaatcg ttgattttag cacctacaag agaattggca atgcaggtag ctgaacaatt
     8821 aagagaattt agccgtggac aaggtgtcca agttgttact gtattcggtg gtatgcctat
     8881 cgaacgccaa attaaagcct tgaaaaaagg cccacaaatc gtagtcggaa cacctgggcg
     8941 tgttatcgac catttaaatc gtcgcacatt aaaaacggac ggaattcata ctttgatttt
     9001 agatgaagct gatgaaatga tgaatatggg attcatcgat gatatgagat ttattatgga
     9061 taaaattcca gcagtacaac gtcaaacaat gttgttctca gctacaatgc ctaaagcaat
     9121 ccaagcttta gtacaacaat ttatgaaatc accaaaaatc attaagacaa tgaataatga
     9181 aatgtctgat ccacaaatcg aagaattcta tacaattgtt aaagaattag agaaatttga
     9241 tacatttaca aatttcctag atgttcatca acctgaatta gcaatcgtat tcggacgtac
     9301 aaaacgtcgt gttgatgaat taacaagtgc tttgatttct aaaggatata aagctgaagg
     9361 tttacatggt gatattacac aagcgaaacg tttagaagta ttaaagaaat ttaaaaatga
     9421 ccaaattaat attttagtcg ctactgatgt agcagcaaga ggactagata tttctggtgt
     9481 gagtcatgtt tataactttg atatacctca agatactgaa agctatacac accgtattgg
     9541 tcgtacgggt cgtgctggta aagaaggtat cgctgtaacg tttgttaatc caatcgaaat
     9601 ggattatatc agacaaattg aagatgcaaa cggtagaaaa atgagtgcac ttcgtccacc
     9661 acatcgtaaa gaagtacttc aagcacgtga agatgacatc aaagaaaaag ttgaaaactg
     9721 gatgtctaaa gagtcagaat cacgcttgaa acgcatttct acagagttgt taaatgaata
     9781 taacgatgtt gatttagttg ctgcactttt acaagagtta gtagaagcaa acgatgaagt
     9841 tgaagttcaa ttaacttttg aaaaaccatt atctcgcaaa ggccgtaacg gtaaaccaag
     9901 tggttctcgt aacagaaata gtaagcgtgg taatcctaaa tttgacagta agagtaaacg
     9961 ttcaaaagga tactcaagta agaagaaaag tacaaaaaaa ttcgaccgta aagagaagag
    10021 cagcggtgga agcagaccta tgaaaggtcg cacatttgct gaccatcaaa aataatttat
    10081 agattaagag cttaaagatg taatgtcttg agctcttttt tgttttcaat aattgattct
    10141 ctgtagatat caaagtacta acgttttaaa ggttaaatat ttaattggat tgagatctgt
    10201 atgcggttat atcattctgt gtaaatatgg ttctccacca aatgtggtga gtatataatt
    10261 taaagaacta tttttaaatt aagaataatc gaacataaat aaactttatg aaatttcagt
    10321 atcatgttct tataaaaaac aatagggctt tttgctgacg ctagtgcgcg ataaataata
    10381 agttgaatat aaaaaagatc actgccaatc attcgtttaa tggcagcgat cttttttatt
    10441 taattatttc tctttccact gcaacatttg ataaccaatg cgtggatgtg ttttaataat
    10501 atcttttgcg tcctcatgac attgtgaaag tttttgtctt aatgatgtca tatgcactct
    10561 taaactaggc atttcagtct tattaacata gccatatatt cgttttaata tcatctcata
    10621 agtgagtact tttcctttat gatttgacaa tagttctaac aagctaaatt catttggcgt
    10681 caaatgtacc tcctgattat taataacaac agatttggag ccaaagtcga tgcttagcaa
    10741 accgttagta aatacaatgt tagtttcttg atgtgactta gcgattctct cgatgactcg
    10801 tattcgtgcc cgaagctcat caacattaaa aggtttagtc atatagtcat tcgcaccgtt
    10861 atctaaagct tgaataattg tttgttcttc ttgtcttgca cttattacaa tgataggaat
    10921 gtcagtatgt tgcctgattt ctgaaatcaa acataatcca tctttatctg gtaaacctaa
    10981 atctaataaa atgacatctg gtttatcaat ttgaatttta aagtgtgctt gtgtggcatt
    11041 gtcggctgta gttacattgt aataatctaa agttaatgca acatcaagta aatgtgtgat
    11101 tgcgtgatca tcttcaatta tcaatatttt agattgcatt atacgtctcc ttcgttaaag
    11161 tctgtatata tattgaaata gaatatactg ccgtgtggtt ggttcggttt atattgtaag
    11221 tttgattgat gtttgtgtag gatagtctgt actaaatata agcctagtcc catgctttct
    11281 ttttggttat ctttaaaata tttatttgat cctgtgtaaa aaggctcgaa tatcttttgt
    11341 tgttcttcta aactaattcc aggtccttcg tctataacgg caaattcgat ttgttcatag
    11401 ctagcataac gaatagataa attgattttg gtgtcagtag aagtgtgttt aactgcattt
    11461 tcaatcaaat tgaataaagc ttgtaaaatc aacttactgt caatgtgtat aaactgtaaa
    11521 tttactgagg atgatacagt tatacgcttt tttaaatggc gacgttctaa aatcatatcg
    11581 atttcttcta ctaattcact aacgagataa ggttgcaatt ttatctgaac atttgatgac
    11641 tgtaattttg ttaatgataa aatatttgtc actaatagat ataaatactg actttcttga
    11701 aaactatgta caagtaattg ttccttttct atgatagaca tatctttact atgtgatact
    11761 aaaatatcta aatttcccat aattgttgtt aacggtgtac gtatgtcatg cgaaattgat
    11821 cttaaaaaat ttgaatgtgt cagttgacgt tcagcctgta acatggattc tctcgtttgt
    11881 ttaagtaacg tcacattttc aacggcgaga gaaagttcat ttaacattga ttctaatatt
    11941 gatgcatcat atggattaat cacttgagaa ctttggtaat caatggctag aatgccttta
    12001 atcggagatg tgccaattgg tatcaaccat ttattaatgc ctggaaatgt atctgttgtt
    12061 gcaccagctt gtctttcatt tttaattacc cagcttaatg cttgttcatg ctgttgagtc
    12121 gtattatcga tatggttttg caatggtatt gttttaatta ctttcgattg attgataacg
    12181 tatatagtaa ttgattgttg caataattga ttaatttggt atccagcatt tattagtaag
    12241 ttttcaactg tataagtttg tttaatcgaa tcattaaatt gaaataataa atctgtacga
    12301 taaagttgct ttttagtaat ggagtattgg aatttaattt gttttaataa agcactcgtt
    12361 aaaatacttg ttaaaat
//
LOCUS       DANRDI010000073        12311 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.073, whole genome shotgun sequence.
ACCESSION   DANRDI010000073 DANRDI010000000
VERSION     DANRDI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.073"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..533)
                     /locus_tag="PZ377_001552"
     CDS             complement(<1..533)
                     /locus_tag="PZ377_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDM6358426.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRK"
     gene            842..1045
                     /locus_tag="PZ377_001553"
     CDS             842..1045
                     /locus_tag="PZ377_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358427.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            1094..1390
                     /locus_tag="PZ377_001554"
     CDS             1094..1390
                     /locus_tag="PZ377_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDM6358428.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(1462..2682)
                     /locus_tag="PZ377_001555"
     CDS             complement(1462..2682)
                     /locus_tag="PZ377_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6358429.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(2675..3225)
                     /locus_tag="PZ377_001556"
                     /pseudo
     CDS             complement(2675..3225)
                     /locus_tag="PZ377_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            3338..4198
                     /locus_tag="PZ377_001557"
     CDS             3338..4198
                     /locus_tag="PZ377_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDM6358430.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            complement(4553..5086)
                     /locus_tag="PZ377_001558"
     CDS             complement(4553..5086)
                     /locus_tag="PZ377_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HDM6358431.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
     gene            5177..5920
                     /locus_tag="PZ377_001559"
     CDS             5177..5920
                     /locus_tag="PZ377_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDM6358432.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SESEEIMMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDSNYRLSNVDTMKVTLYS
                     NGSNYDKEALLINKDEFCPLRKITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPM
                     TFENKRDIVQDISKIFEVNYYTYNHIMHH"
     gene            6354..6956
                     /locus_tag="PZ377_001560"
     CDS             6354..6956
                     /locus_tag="PZ377_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358433.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            7223..10375
                     /locus_tag="PZ377_001561"
     CDS             7223..10375
                     /locus_tag="PZ377_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HDM6358434.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            10432..10914
                     /locus_tag="PZ377_001562"
     CDS             10432..10914
                     /locus_tag="PZ377_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358435.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
     gene            11119..12105
                     /locus_tag="PZ377_001563"
     CDS             11119..12105
                     /locus_tag="PZ377_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HDM6358436.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
BASE COUNT         4626 a         1606 c         2053 g         4026 t
ORIGIN      
        1 tctttacgtg tacgaagatg aatagataaa ttggcaatgt cttgttcgaa gacgtgcttc
       61 aaccaatctt tccattcatc gatttcatag tagccaaggc gtgttttaac acttaccgga
      121 agcccacctg ctttagtcgc ttgaataatt tcggcagcaa cgtcaggtct taagattaag
      181 ccggaaccct tacccttttt agcaacattt gctacaggac atcccatatt taagtctatg
      241 cctttaaagc ccattttagc taattgaata ctcgtttcac ggaactgttc tggcttatct
      301 ccccatatat gagcgaccat cggctgttca tcttcactaa aagttaagcg tccgcgcaca
      361 ctatgtatgc cttcagggtg gcaaaagctt tcagtatttg taaattcagt gaaaaacaca
      421 tccggtctag ctgcttcact tacaacgtgt cgaaagacga tatctgtaac gtcttccatt
      481 ggcgccaaaa taaaaaatgg acgtggtaat tcactccaaa aattttcttt cataatatat
      541 ttataccctc tttataatta gtatctcgat tttttatgca tgatgatatt accacaaaag
      601 cctaacttat acaaaaggaa tttcaataga tgcaaccatt tgaaagggaa gtctaagagt
      661 agtctaaaat aaatgttgtg gtaagttgat caatacaaag atcaaggatt atagtattaa
      721 attgttcatt attaatgata cactacttat gaatatgatt cagaattttc tttggctact
      781 tttacagtaa agcgattttt tagttatctt ataacaaaga caaatttata aaggtgatat
      841 tatggaaggt ttaaagcatt ctttaaaaag tttaggttgg tgggatttat tttttgcgat
      901 acctattttt ctgctattcg cataccttcc aaactataat tttataacta tatttcttaa
      961 cattgttatc attattttct tttccatagg tttgatttta actacgcata taattataga
     1021 taaaattaag agcaacacga aatgaatcat taatacgaat gtgattaaac ataaaactga
     1081 aggagcgatt acaatggcga ctaagaaaga tgtacatgat ttatttttaa atcatgtgaa
     1141 ttcaaacgcg gttaagagaa gaaagatgat gggagaatat attatttatt atgatggcgt
     1201 ggttataggt ggtttgtatg ataatagatt attggtcaag gcgactaaaa gtgcccagca
     1261 gaaattgcaa gataatacat tagtttcgcc atatccaggt tctaaagaaa tgatattaat
     1321 tctagacttt accgaagcaa caaatctcac tgatttattt aagaccataa aaaatgattt
     1381 gaaaaagtga agtagtgaag tgtgggtgca gagagaacta agcccatcga taaatggtcg
     1441 cttgttaaag aagagtgacg gtcactcttc tttatgtgca tattttattt tgcctgtttt
     1501 gttaacaagc agcagtgtaa caaatatgag taaggataaa atgagtataa tatagaaacc
     1561 gaatttatca ttaatttcat taatccatct tcctaaaaat ggagcaatta aactttgcag
     1621 taacaatgaa attgacgtcc atatcgtaaa tgagcgaccg acatatttat ctgaaacagt
     1681 gttcattata gctgtattca tataaattct gattgatgaa attgagtagc ctagtataaa
     1741 tgatcctatg aataagtaaa atgctgagtt tatccaaata aatagtgctg aatttatgac
     1801 taatatgaaa tataacaaaa atatcaatac tttagttgag attttcttcg aaagaatagc
     1861 tgaaattaaa cctgcacata atcctccaat gccatataac atatctgaaa aaccaaattg
     1921 tacagacgaa agttttaaaa cattataaac atatcctggt aatgatatgt taaagatcat
     1981 tgtaaacacc attggtatga ttgaaataac tccaaaaata aatatcatca tgttgtcttt
     2041 taaaaatttc catcctaata aatattcttg caataagcta tttgttgatt cttcctctga
     2101 atgagttggt ttatctacat gcaatctaaa taacataaaa atgctgatta gaaacatcat
     2161 tatagtcatc gctataatta gagtgaatcc atttatttta tataatattc ctgataatcc
     2221 acctgcaata aacatacctg tttgcaaact aatctctaat agagaatttg catctgtata
     2281 ttgatctggt tttaatatct gtttaactaa acttctagat gttgccatat acgtagtcca
     2341 acctatccca ttaacaatcg caaatccaat cactaaatag gtctcaaatc ctatcattac
     2401 gagtgctatg acaatgagta aatataatat tacctgaaga agataggtga ttaaaataat
     2461 atttcgtcta ttatatttat cggctagtcc acctattata ggagaagcta agaaaccgga
     2521 taatacattt aaagctaaca taatacctaa tagttgggag tcgttagttt tgtcgattaa
     2581 ataccaatta gccccaactg tactaattcc aactccgaat gctgaaatta tttctgctaa
     2641 aaagaacaat ctgaagtttt taattttcaa caaatcagtc ataaatatca ccttttttca
     2701 atgcaagatt tatatttgcg agctcttcta taaattgaga gtcgtttaaa atatcgaatg
     2761 gtttattgcc ttcgccgatc aaaaatctat agttagtaat attcataaac tcaaatatta
     2821 atttgaattg gttgactaaa ggttgtgctt tggtatgcgg attgtcgcca cctatgatta
     2881 aaatcaaata ctttttttgt gacatgattt ctttgaagtt gtctatttgc gtatctcgca
     2941 atgactcggt ccatctatca atgaagagtt ttaaagacgc actcatggag taccaataga
     3001 gtggtgttga aaaaataata atatctgatt ctaaaacttt gattaaaatt tgttcgtaat
     3061 catcattatg aaagttggca cctttgctat gacgattatc tgtaaccttt tcaatgttgc
     3121 tttgatataa attcacaaag ttgactttta aattcttcaa tagattctct actgcgatag
     3181 ctgaattgcc acctttctac tacttccaaa taaacaagta atcatagtca taactccttt
     3241 cgatttacct ttataataat atttaatata ttattattaa atcagaattc ttagaattca
     3301 ggattcgata aaagggaacc ctaaaggagg tactactttg aacttaaaca ttttaaaatc
     3361 atttttagta actagtgaaa caaagaacct tactaaagca tcagagttac ttaactattc
     3421 acagtcaact gtatctacac atattgaaaa attagaaagg caattagatg ttaaattatt
     3481 ttatagaaaa aaatatggta tggaactaac ggaagaaggc ttagcatacg ttaaatacgc
     3541 taaagtgatt ttagatagta atagcgaata cgagagagaa ataaaaggac tttacaataa
     3601 gaaggtaaat ataagtatta acatgcaaga aagtcagtat ttgtatcgct actataataa
     3661 gattagtgaa tggttagctg aacacccata tgtaaacttg aagtttaaat ccgcacattc
     3721 taatttctat attaaagaag aaattgctaa tttcaaatcg gatattagcc ttatcacaga
     3781 cgaaaagatt attaatagta acttaactgc tattcctata actgaagaac gtttaatatt
     3841 tgttactaac aaagagatga aagatttcaa attaaacgat attcaaaacc atacattact
     3901 tgttactgaa aaagggtgta gttacagaga acagttagaa atgattttat ataaatatag
     3961 cttttcaaca aaacaaatga ttgaatttct aggaattgag tcattaaaaa aacacttgaa
     4021 aaattcaggt gggattgcgc tattgccgga atttattgtt gcagatgaat tagaaaataa
     4081 aagtttgttt caaatagacg tagatgtcga tattccgaat ttagaaacaa cattgataat
     4141 taatcctgaa tcgaataagc aagtacttga atcttttgta aaagatgttt ttttataatt
     4201 attggtgaaa acgtgtagtt atggtgaaac tcaaagataa taatttaaat gagatgttaa
     4261 tgaaaaagta attcaatata aaaacaggtg atttatatct ttaataagga ttatttctag
     4321 ttgaaatttc aaattgcggg cattcatttt caaaatagta aagaatttaa tagatgaaaa
     4381 cctttgaatc atactagtta gtatggattg attaatattt taacctgcta aatgagtagc
     4441 ttatgtattt taaatagctg tcatctagca ggttctttat tattaataag ttcagataaa
     4501 ttattttagt ctgttttcta atgttgataa taataacttt aaatctgtac tttcattgtg
     4561 agatatatag tacatcataa atttttatat ttggattgag actctttgga agattttcaa
     4621 taaatgaata tttcacaaac ttccacttat caggattata aattataatt tctgcaactg
     4681 cattatcgaa gattctaaac aattttttga tttctatttc ttttagagat aaagtaggga
     4741 aaatgtcgat atattcttca agcgcataaa gactatgttt aacagaatcg gttatgttca
     4801 ttgaatatat cacaccaata tttatatttt gccatccaaa agaagaaata acattatcat
     4861 aaaatccatt gaatataaat tcagtcatcc cctctgaact tctccataaa tataatggtg
     4921 aataagtatt tgtaatgcaa ttagtagttg tttgacttat taaataagct tttataatta
     4981 aatttttaaa tccatcagtc ttataaccat ttaatcgaac tctatctcta attatttcca
     5041 tattgtaatc actaggcaac ttaacatgat attgcattgc atgcatttag cataccccct
     5101 tttataaaaa ggatagcaat aataagtaaa atctcatatt atccaattgt gatatagtta
     5161 tcataaaaag tgataggtga ttaaattgaa ctttaatgat ttggaaattt ttataactgt
     5221 atgtgaagaa gcatctatca ataaagctgc aattaaactt agatatgcac aatctaatat
     5281 atctcaaaga attagcaagc ttgaaaatga attaggtgta gttttgcttt ttagaaatca
     5341 aaaaggtgct aaggcaacta aagcaggcga agaattctta gcgtatagca aaaaagtatt
     5401 aagagataca gagactataa aaaataaaat gaaaaataat actatgtcta ttttatgctc
     5461 agaactgtta tttaattatt tatctgagag cgaagaaatt atgatgtcga ataactcaat
     5521 taattttatt tctagtggaa atattagaaa agctatagaa aaaaataatt atgataaggt
     5581 tatttcattc ataaaaatta acgactcaaa ttatagactt agtaatgttg atactatgaa
     5641 agtaacgctt tacagtaatg gaagtaatta tgataaagag gctttactaa taaataaaga
     5701 tgagttttgt cctttaagga aaataacttt agataataag cttgattcac aacgggtaat
     5761 ggaaattgat tcattggcag caataataaa cttagttaaa caaggaaaag gaaaggcttt
     5821 attacctatg acttttgaga ataaaagaga tatagtacag gacatttcta agatatttga
     5881 agttaactac tatacttata atcatattat gcatcattaa ttgaaagttg attgagttta
     5941 aattacagga acgattttaa aagtctagta gttagaatga gcgatgtgac agaaaagaaa
     6001 ttatgccttt tattttgtaa attcgttgtt ttaaagtaaa ggtccttgta gtataggaaa
     6061 aagtggatga cttctccgcc tttaccaccc tttcaagcat aatgataact gctaaatatt
     6121 gattatagca ccattgcaaa actaatcatt aattgtcata ttcaactatt ttgaaaagta
     6181 tcaatttcac attatcaaat tattgagtat ctcaatttat tgttatacac aaacagtatt
     6241 tttgttatca tagagttaat aaatataata aattaaacaa gtaactttac aaaaattaga
     6301 atgattcaat tcaatcataa ttgtttgaaa atagaggtga gcaggtgaac gatatgttaa
     6361 ttagtcttgt aatcccagtt tttgctttgt tagttattgg tggtattatt tggatgatta
     6421 tagaaggtat agtacatatt tcaaaaaaga ataaagcaat tgataacttt tttaaccaag
     6481 ttaataaagt aagtgagaca tataaattcg ctactacttt tttatttcta atcttggcta
     6541 cggctggtat ttctcaattt tatctatatt atatagtatc agcgtttctt ttttggcttg
     6601 tcatacttac ctttggcatt gcaggtatca tttttttaat gccatatgga ttatgttttc
     6661 taccgtttta taagcaaaaa aagaaaaaac agacatttaa aaaatacatg gtttacacta
     6721 cgattggttt gtcaatttgt ctaggcttat ctctagtttt ggttcacact acgaaaattt
     6781 atatggacga aggtggcgta agatactatt acggtagttt tgtaatgaaa caagcgggcg
     6841 gttatgctta tttagcttta gcggtacttt caacgttgtt aattgttgcg aaaaaagcta
     6901 caaataaaaa taaagaaatc gaaaccgtcg acaatacaaa tataacggaa agataattaa
     6961 gggagtgctc attcaggagt gctctttttt gatgtccaaa tttagttgca aatgaaggca
     7021 tagaagaaat ttttatagtc atttattgga taatgaatta tggtcatatc gattaatttt
     7081 attagtgagg attttacaaa catttgttga ataaatatgg taatgataat atatgaatat
     7141 ttttgaaaaa acactaatat gattaaatta tttgttaaaa tagcattaag taaaaaacaa
     7201 atagggaaca aggtgggatt tcatgagtca attgctaaat gatacgttat cggcttggtt
     7261 gttaattgaa tctttaagtc caggagaagt aaattttaca gcggaagata tactctcagc
     7321 tgaacatttt aaaaatggtg caaagcaagc acaacttcaa agttttgatg aatattttga
     7381 aatatggaat gctgaacgct ttattatatc agaagaaaaa tcagagactg gggaacttat
     7441 atttaaattt tatagacatt gcttccgcta taatgaaatt aatttgaaaa ttcaggatat
     7501 ttttgatgat tattctgaga ttcataatcc aaatgggaca cactgttatg gttacacatt
     7561 taacacagat aaacacggca aagtgatagt tgattctata catattccga tgattatgag
     7621 tgcattaaaa gaaattgaaa agaacaaaaa tgccaatata gaagaaaaat ttaatgattc
     7681 tgttgaaaaa tttgttcaaa aagtaaaaga aattttagca gatgaaccaa ttaatgaatt
     7741 taaattgaag aagatggaca aagcttatga tgagtacttt tctgtattaa attcaaagaa
     7801 agatggatta tttggacatt atgtagcaat agaatatgtg aaagatagtg atttaccaca
     7861 gccggaattt aacagcttct tcataagtga tattgagaaa gcaagaaaat ctcccaacca
     7921 aactttaatt gattacattg aaggtgtaga agaaagtcag cacatagaag tagatgaaaa
     7981 taaagaaatg tttgacaaat ttttacatcc ttcacgtttg cctgatggac gatggccatc
     8041 acagactgag tttagattgt ctttaatgca acaacttgct gtaaaccaaa ttacgagtgg
     8101 taatgaaaga ataagttcag ttaatgggcc accaggtaca ggtaagacta ctttattaaa
     8161 agatatattt gctcatctag tagttgaaag aggtaaagag ttagctaaac taaataatcc
     8221 taaagatgca tttgtcaaaa caaaaattca tgaaacggat gataaatacg tatacttact
     8281 aaaggaatct attgccaaat ataagatggt agtcgcatct agtaataatg gagctgttga
     8341 aaatatatct aaagatttac cgaaaattga agaaattata agaaatcccg aaaaatgtaa
     8401 attccctaaa tatgaacaga attatgcaaa tttagcacat gaattaaaag attttgctga
     8461 aatagctgaa gatttgattg gtgaaagtgc ctggggctta ttttctggag tttttggtaa
     8521 aagtactaat attaaccaag tattgagtca tatgttaaaa caagatgcga atgatattgg
     8581 ctttgctaaa ttactacaaa atgagaataa tcgtatgagt tataacgagt taatgagtga
     8641 atggcaatca catcaacgtg catttttaga agagttgagg catgttgaaa tgttaaaaga
     8701 agaatctatt agagcatatg atgtttataa aaattgtgag tctttctcta agattgaaca
     8761 ggttattaat agtgaaaaaa caagtattga agaacaggta tatcatttag ataatgaaac
     8821 gttacgagac aataaagaaa tagaagattt ggataatcga attaattata ttgttaagca
     8881 aatagaaact ttaaatgagt taattaaatc catcaaagaa agcaacaaag gttttattaa
     8941 caaactgaaa gcgatgttta attcagaaga agatgaaagc tataaagatc ataataaaga
     9001 gaagcaacaa ttattaacac aacagttaga gttagagaaa tgtaaaaaaa acaaacatga
     9061 agaccttgtt agcaaactaa aagaaaaaga gaaattaatt aaacaattaa ctaaagtaca
     9121 gttgcaatta gacgagttaa attcacagtt acaagagtta gaagcatatc gtattgagtc
     9181 aaaaattaca attccagaaa aagatttttg gagtgacaac aattatgatg agcgccaagt
     9241 tactaatctg tggacgagtg acgaacttca atacagacgt gccatgctct ttttaagagc
     9301 aatgatattg cataaattat tattgattgc taataataca actatttatt atgcgattaa
     9361 tgattttaaa gatagaagga aattaattga tgcaaatcca gataaagtac acaacgcatg
     9421 gaatgtgatg catttaatat ttccagtagt tagtacgacg tttgcaagct ttaaatctat
     9481 gtatgggggc ataccaaaag atttcataga ctacttattt attgatgaag caggacaagc
     9541 aatacctcaa gcagctgtgg gagcattata tcgttcaaaa aaagttgtag ctgtaggtga
     9601 tccgattcaa atagaaccgg ttgtgacttt agaaagtcat ttaattgata acattcgtaa
     9661 aaattatcat gttccggaat atctagtttc taaagaagct tctgtgcagt ctgttgcaga
     9721 caacgccaat caatatggtt tttggaaatc tgatgctact gatagtaatc aaaaaacctg
     9781 gataggcata cctttatggg tgcacagacg atgtttaaaa cctatgttca cgatagctaa
     9841 ccaaatcgct tataataata aaatggtgtt gccaagtaat attacaaaag taggtaaaac
     9901 aggttggtat gacgttaaag gaaacgcagt tcaaaaacaa tttgtgaaag agcatggtga
     9961 aaaagtagtg ggattattag ctgatgattg gattgaagca attaaggaag gtaaaaatga
    10021 accgagctca tttgtaatat cgcctttttc agcagtacag caacagatta aacgtatgtt
    10081 aaagcaacaa ctaccgacta gaattgatat tgaacgtaca aaaattaatc aatgggtcga
    10141 taaatccatt ggtactgttc atacttttca aggtaaagag gctcagaagg tgtattttgt
    10201 aataggtact gataataccc aagatggtgc tgtgaactgg tcatgcgaaa aaccaaactt
    10261 gttaaacgtt gcagtgacaa gagctaagaa agagttttat gtaattggcg acatgcaaag
    10321 aatacagatg aaaccatttt atgagacgat ttttaaagaa agaaatgtaa aataacatac
    10381 aaaaaagtat atagaggaag ttatacattt taaaaggagc aaaattgaat aatggagaaa
    10441 tttaacaact ggatattaaa tgcaataagt ggatctcaaa cagacaagaa tgaaacaact
    10501 gaagaattaa aaggggcaaa atttatcatt ttatatgcat attcaatgct cgttttgctt
    10561 gcgttagtaa tttctaacat attcattcac attttggagc ctaaactatc aatcaccact
    10621 caaatcatca tcgttttgat tttaattgaa gcactaattg gactgcgttt cttgaaagcg
    10681 tacgatgtta agcgtggcaa agataaagaa aataagaaaa atagtaagga tttcgttaaa
    10741 ctaaaatcaa ttttagtagc aattttattt acatcattgg cgctgacagc aggtactgta
    10801 gctgatatat acggtttcac tgacttagga aatactagaa gtgatttaat cgtttggagc
    10861 ataggtggta ttatatttgg cctcgtatgt tacacaatgg aagataaaag ataacgataa
    10921 ggagctggcg attataaagc tagctccttt tttaacttat atatgtaaag aactatccta
    10981 agggttttta atcatatgtc aataatttct ataatacatt attaaaacat caattaaata
    11041 agttttaaaa ttttacacat atttttattt aaaaaagatg tataattaat gtattaaata
    11101 tagaaagaag ttgatattat gaaaaagtgt attaagactt tgtttttaag tatcatttta
    11161 gtagtgatga gtggttggta tcattcagca catgcgtcag attcgttgag taaaagtcca
    11221 gaaaattgga tgagtaaact tgatgatgga aaacatttaa ctgagattaa tataccgggt
    11281 tcacatgata gtggctcatt cactttaaag gatccagtaa aatcagtttg ggcaaagact
    11341 caagataaag attaccttac ccaaatgaag tcgggagtca ggttttttga tattagaggt
    11401 agagcaagtg ctgataatat gatttcagtt catcacggca tggtttattt gcatcatgaa
    11461 ttaggaaaat ttctcgatga tgctaaatat tacttgagtg cttatccaaa cgaaacaatt
    11521 gtgatgtcta tgaaaaagga ctacgatagc gattctaaag ttacgaagac atttgaagaa
    11581 atttttagag aatattatta taataacccg caatatcaga atctttttta cacaggaagt
    11641 aatgcgaatc ctactttaaa agaaacgaaa ggtaaaattg tcctattcaa tagaatgggg
    11701 ggtacgtaca taaaaagtgg ttatggtgct gacacgtcag gtattcaatg ggcagacaat
    11761 gcgacatttg aaacgaaaat taataatggt agcttaaatt taaaagtaca agatgagtat
    11821 aaagattact atgataaaaa agttgaagct gttaaaaatt tattggctaa agctaaaacg
    11881 gatagtaaca aagacaatgt atatgtgaat ttcttgagtg tagcgtctgg aggcagcgca
    11941 tttaatagta cttataacta tgcatcacat ataaatcctg aaattgcaaa aacgattaaa
    12001 gcaaatggga aagctagaac gggttggctg attgttgact atgcaggata tacgtggcct
    12061 ggatatgatg atatcgtaag tgaaattata gatagtaata aataaggatt caataatgat
    12121 attaagacga gtatgaaaat agttagattc taattatttt cactactcgt ttttattttg
    12181 aaaataagta ataattcaac aatattataa attgaacaga ttgtttgtga aatttttgat
    12241 aatattaaag tgaaaaagtg ttataaattg ataaatatat gtaattaaca aaaacaaatc
    12301 attttaaaaa g
//
LOCUS       DANRDI010000074        12197 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.074, whole genome shotgun sequence.
ACCESSION   DANRDI010000074 DANRDI010000000
VERSION     DANRDI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.074"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1072
                     /locus_tag="PZ377_001564"
     CDS             <1..1072
                     /locus_tag="PZ377_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HDM6358437.1"
                     /translation="IFANKCLANNDVQIMNSIKKLIEAELLTTTNDFEVSIYKKTRPE
                     LQSILKSFGIKTTGNKPDLIKRIDDNFHIINNLDLPYVYIPTKKGEEILKKTEYLTSF
                     IQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEFQRKYDNGEFDFNHGYNFELNML
                     IDHYKRDVKDYDNARKYSNIYLYFGLRDFLKKLMSNYSYYDSKGNIDLNEIQNDLNRF
                     INSSASGMYERLIYNENLSNNIMFELFKKDTQDYSDLEEQLIEKFINYVVSNVKKESR
                     SNTLIELSKILENGYTIDKEEFKKEDDYLSKYIFTDIDYLKKLESKINVAIDIRSGEI
                     HLVLDDDSLDILIQNQKYGNEF"
     gene            1115..1598
                     /locus_tag="PZ377_001565"
                     /pseudo
     CDS             1115..1598
                     /locus_tag="PZ377_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1759..1863
                     /locus_tag="PZ377_001566"
     CDS             1759..1863
                     /locus_tag="PZ377_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358438.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(2363..3454)
                     /locus_tag="PZ377_001567"
     CDS             complement(2363..3454)
                     /locus_tag="PZ377_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDM6358439.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(3720..4700)
                     /gene="czrB"
                     /locus_tag="PZ377_001568"
     CDS             complement(3720..4700)
                     /gene="czrB"
                     /locus_tag="PZ377_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDM6358440.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(4702..5022)
                     /gene="czrA"
                     /locus_tag="PZ377_001569"
     CDS             complement(4702..5022)
                     /gene="czrA"
                     /locus_tag="PZ377_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDM6358441.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            5174..5839
                     /locus_tag="PZ377_001570"
     CDS             5174..5839
                     /locus_tag="PZ377_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDM6358442.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            6274..7212
                     /locus_tag="PZ377_001571"
     CDS             6274..7212
                     /locus_tag="PZ377_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDM6358443.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            7243..7473
                     /locus_tag="PZ377_001572"
     CDS             7243..7473
                     /locus_tag="PZ377_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358444.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            8145..9518
                     /locus_tag="PZ377_001573"
     CDS             8145..9518
                     /locus_tag="PZ377_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDM6358445.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            9976..10389
                     /locus_tag="PZ377_001574"
     CDS             9976..10389
                     /locus_tag="PZ377_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HDM6358446.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            10589..11032
                     /locus_tag="PZ377_001575"
     CDS             10589..11032
                     /locus_tag="PZ377_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDM6358447.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(11153..11863)
                     /gene="deoD"
                     /locus_tag="PZ377_001576"
     CDS             complement(11153..11863)
                     /gene="deoD"
                     /locus_tag="PZ377_001576"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDM6358448.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
BASE COUNT         4472 a         1630 c         2134 g         3961 t
ORIGIN      
        1 gatatttgct aataagtgcc ttgctaataa tgatgttcaa attatgaatt caataaaaaa
       61 attaattgaa gctgagttat taacaacgac taatgatttt gaagttagta tatataaaaa
      121 gacaagacct gaattacaaa gtattttaaa aagttttggt ataaaaacaa caggtaataa
      181 acctgactta attaaacgta ttgacgacaa ttttcatatt attaataact tagatttacc
      241 atatgtatat ataccaacta agaaaggaga agaaatttta aagaaaacag agtacttaac
      301 ctcttttata caaagttatg gtgaaatttc tcttgagcgt gcttattatt tggttgaaaa
      361 ctatatagat gaaaattgcg atgataaagt tgcagaaata tacaagtttg aatttcaaag
      421 aaaatatgac aatggcgagt ttgattttaa tcatggatat aatttcgaat tgaatatgtt
      481 gatagatcac tataaaagag atgtaaaaga ctacgataat gccagaaagt attcaaatat
      541 ttatctttac tttggtttga gagatttctt aaaaaaatta atgagtaatt attcatatta
      601 cgatagtaaa gggaatatag atttgaacga aatacaaaac gatctgaata gatttatcaa
      661 ctctagcgct tctggtatgt acgagcgatt aatatataat gaaaatttat ccaataatat
      721 tatgtttgaa ttatttaaaa aggacacaca agattatagt gatttggaag aacaattgat
      781 tgaaaagttc ataaactatg tagtgtctaa tgtaaaaaaa gaaagtagga gtaatactct
      841 tatagagtta tcaaaaattt tagagaacgg atatacaatt gataaagaag aatttaaaaa
      901 agaagatgat tatctttcta agtacatatt tactgacata gattatttga aaaagttaga
      961 atcaaaaata aacgttgcta ttgatattcg aagtggagaa attcatttgg tattagatga
     1021 tgatagcctt gatatattaa tacaaaatca aaaatacggc aatgagtttt gagtcataac
     1081 taaatataat atgttgaaga gaggttttat ttaaatggct aaaattggtt atgatcgtgt
     1141 atcaacgaaa gattaatatc gatacactta aagaatttgg gtgtgggtgt gtatatagtg
     1201 aaagaataag tggtgtcaaa cgtacagagc ttgttaagtg tttagattat ttacgagaga
     1261 gcgacacatt agttgtctat caacttgatc ggttaggtag aacgacaaaa ctattaattg
     1321 aattatcaca atgattcgat gataacggaa ttgacttaca aattagtaac atgaacattt
     1381 caacgaaaga cacaatgggc aaaatgtttt ttacgatgat gagtgcattt ttcaggatta
     1441 gaagttaatt tactatgtga gtgtataaaa tagacttagc agcaacaaga gcgagaggcc
     1501 gaaaaagcgg gcgcccctct ttaccagaga ataaaaaatg agaaattaaa tttttatatg
     1561 atgaacaaac gataacaggg gaagaaatag ctagttagac aagggtatgt cactcaactg
     1621 tttatcgagt tgattaagaa atgaaaaaac ttatactatg aattactgtt taaaagtgtg
     1681 catgttataa tatttattga gcaagttgga tagatggtgg ctaatctctt aataaagggg
     1741 tgatgcctat ggttatagtt gttactccta gaaaggacta gcatgtctga ttttgaaatg
     1801 cttatggttg tattaacaat cattggttta gtattgatta gtactcaaga ccataaaaaa
     1861 taaccttcta ttcgctttga ccggcatttt tgaaggctat ttttaaataa aatataaggt
     1921 caccgtcttt ttaacgggct cattagggta acatgtttcc gagtgttgcc ctttttgtgt
     1981 ttcaagagtt aatgattttc atcttttgct tctacttact acaaatatat tttaacacat
     2041 ttttcttatg aattgtagtt ctgaacataa tcagaattaa taaaaccaac tttccataca
     2101 gcagaaaata caattaaaag tatagaatgt aatcacgaat tatataaaaa gtattggaga
     2161 cctcttcata tatagataat tcacttagtt attttagaaa gaagccccta acaactaaag
     2221 ttgaaaaata gaggaacaca gttggatgct acgcatcaac tgcataagag cccctaacaa
     2281 ctaaagttta aaagtaggag cgcctgcgct aagcgcatgc ataagagccc ctaacaacta
     2341 aagttgtaag gggctctaaa atttattttg gttgattgtc ttctggttta tctgaagtca
     2401 ttgtttttgt tgtattatca tttaatgaat cttgtgcttt ttctgttttt gtttcaacag
     2461 atgttgtcac tttatcattt tctacttttg tattttttgg ttcagtgaca acttttttat
     2521 tcttgtcagc tgtttttact ttatctttag taattttttg accacgtttt aagtaatatt
     2581 gaacgatacc cattaaaatg attgcatgaa taacaacgaa taaaataatt gtcattactg
     2641 tataaactct tatgatattt tctgcaacac tttgagagag tgattgcgta ttaaatgaaa
     2701 tcaagtaacc aacaggtgtt ttaaataaaa taataattaa gtttaataat agtattccaa
     2761 tgaaaaattt gaaaaatgtt ttttgaccat ttttcattgc tttaaatcca ttagctaagt
     2821 gtgtttttac tttatcgtta gttgattcaa cgaaactagt cataaagttg aaaataggta
     2881 ttaacaataa ccaagtaata attgctttaa tcaataaaac aacaatgatt atgatgcttt
     2941 gagttgtaat agcaatacca atcaggtgtg tactatctgc atatgatgat tgtactgaat
     3001 tcataatcat ttctgatact ggagaaaata atttgtatag taatgatgtc agaagtgaaa
     3061 gaacgataat cattgctata gatacaagac cgatcagaac gctctttaaa taattacctt
     3121 ttttaaatgc aataaataaa tcggtaaatt ttactttttc atgactcatt gctcgtttca
     3181 taacatttgt aattccgata aaaatttgaa ctaaaacaaa cagtgaaact acagctgcta
     3241 taagtaatac agcgataact tttaaatatg catctactgg tggtttttgc ccaaattgtg
     3301 aataaattgc aatagtttgt gcatacttag caagctggaa attagcaagt aaatatacaa
     3361 ctgcaattac agcaaatgct ataagtgcat atatcaatgt tattaataat tgtggtttag
     3421 catttttaaa tgctgattta aagtaagtaa acaaagtggt gcctcctttt cttcatataa
     3481 atacaataac aaaaaacacg ttattttcat attaatatct aataaaaatt aattaaagtg
     3541 actaaagtta gagaattata atagtaaatt gtcaaaaata tgactaatgt aagaaataat
     3601 agttgaaaat ttgttaataa acattgcatc atcaaagacc aaacaagtcg ccataaagac
     3661 ataaaaaacc tactgccatt gaaaagttta atcgatgcaa tggcagtagg ttaaaattat
     3721 taatgtacat gcgcgtgatg atgagcatga tggttatgtg aatgactatg tgttcctgaa
     3781 catataatcg attcatcatg tttgtgatta ggcgtttcta attgaatagt catatggtga
     3841 atatttaaat gtaataaatc atgctcaatg ttttctaata ataattcaca ttctttcatt
     3901 gtcaatgtat ggtctacaac aacatgacaa ctcaatgcat tcatatcatt tgaaattgtc
     3961 caaacatggc aatcatgcac actttgtatt cgtgaatcct ttttaatagt agttataact
     4021 tcatctatat caacatcact tggtgtgcct tccattaaaa tgttaattga agattttgtg
     4081 ataccccaag cactttttaa aataataacg gaaactaaaa tacttgcgat aggatcggcg
     4141 attgtccatc caaatgccca aattaaaata gctgcagtaa tggcgccaac tgaacctaat
     4201 aagtctccga taacatgtag aaaagcacca cgcatattta aattgtgtga agtgtcgccg
     4261 cctttaaaca taaagaatgc aacaacgata ttgacaatta aaccgataat actaatgatt
     4321 aacatttctt ttgattgaac ttcagaagga acaaagaaac gtttaatcgc ttcaaaaaca
     4381 atcaaaatac ttattacaaa aagcgttaca ccgttaaata acgctgcgag tacttcgaaa
     4441 cgtttataac caaatgtttt tgtagttgtg gcattctttt cagcataaat aaatgcgaca
     4501 agtgcaacac ctaatgagaa tgtgtcgcta aacatatgga taccgtcaga tagtaatgcc
     4561 aagctgttag caaggagacc gccgatgatt tcgataaaca tatatagacc gattattaaa
     4621 aacgatataa acaatacttt cttattattt gtagttacat gactatgcat atggtcatga
     4681 tgatgatgtg aatgagacat attataaccc actttcttta ggatgattcg cgtgatgtat
     4741 ggcttgcttt aacatagttg ctacgtggat gtcatctaat gaataaatca ttgattggcc
     4801 ttgtcgtttt gctttcacaa gatgcacact tttaagtaat tttaattggt gcgagacatt
     4861 tgattgagat aaattcaatt gatgtgaaat gtgaccaaca cttgcttcgc tgactgataa
     4921 caattccatg atacgtattc gattgtaatc gcctaatgcc ttgaaaattt cagttacgcg
     4981 ttctaatgta tctgtattta tttctgaata ttgttctgac attatatgac aatcctttca
     5041 tgtgaatatt tgttcatata ttaatttaat acgttcccag tagtatgtca attgattttt
     5101 atagttgtaa caagttttac atatctcata aagtggtatg gcatagagaa taataaatag
     5161 gagagtgttg taaatgaata ttttggttat aggtgctaat ggcggtgtag gttcgttact
     5221 agtgcaacaa ttagcaaaag aaaatgtacc atttactgct ggtgttaggc aatcagatca
     5281 acttaatgcg ttaaaatcac aaggtatgaa agcaattctt gttgatgttg aaaatgattc
     5341 aatagagact ttaactgaga cgtttaaacc attcgataaa gttatctttt cagtaggttc
     5401 tggagggaac acgggtgcag ataaaacaat tattgtcgat ttagatggtg ctgttaaatc
     5461 aatgattgcg agtaaagagg ccaatattaa acactatgtc atggtttcaa catatgattc
     5521 gagacgtcag gcttttgatg atagcggtga tttaaagcca tatacaatag ctaaacatta
     5581 cgctgatgac tatttaagac gttcaggttt gaattataca attttacatc caggggcact
     5641 tacaaatgca gcaggatctg gaaagataga agctgcacaa tattttgatg gtaaaggtga
     5701 aattcctaga gaagatgttg cgacagtatt aaaagaaatt gtcacatcca atcattttaa
     5761 tcaccaagag tttcaaatta ttagtggtga tcaggacatt aaagatgctt taactcaatt
     5821 tgaaaatgaa acggattaat aaggatagta aatatgattt gaagcgggtt actttttcgt
     5881 aactcgttat tttttgaaaa tgatgaatta taagtatgaa taagtacgta ttgtctatgc
     5941 gttttacaaa gaatttgaac aatgtatggc acaactatta aagttgttta ttaattggta
     6001 taatgggcga gtaaatttaa agggaaatat gtacaaatgt aagagagata taatctctat
     6061 gctataaatt tttgaaaatg ttaaagtact gtaaatttaa gtgaagcgct ttaattggca
     6121 gtactgcaat gttagtaaat atggtacaat cactaatagt taatttgaaa tgtggaagtt
     6181 aataatacga tgtaaatgag aacaaaatca aaatataatg gaacaacaac atcagagttc
     6241 tattgcttac ttaatcacaa tggaggtgca acaatgccat tatttttaca accaatttta
     6301 aaaacaaaat tatggggcgg tcaacgtcta agtgagtttg gatatcaatt agacaatgat
     6361 acaactgggg aatgttggtg tgtgtcagca catccaaatg gtacgagcga gattattaat
     6421 ggaccatatc aaggtcaaac attagaccgt atttggtcag aacatcgtga attgtttggt
     6481 gatttcccaa gcaaagattt tccgcttcta actaaaatag tggatgcaag agaatcactt
     6541 tctattcatg tgcaccctga taattcttat gcttatgagc atgaaaacgg gcaatatggc
     6601 aaatctgaat gttggtatat tatagatgca gaagaagatg cagaaatagt tatagggaca
     6661 ttagcagagt ctagagaaga agttgcgaat catgttcaac acggaacgat agagtcgata
     6721 cttagatata ttaaagtaaa acctggagaa ttctatttta ttccagcagg aacagtacat
     6781 actatttctt caggaatatt agcatacgaa acgatgcaat cgtcagacat tacatataga
     6841 ctttatgatt tcaatcgtca agataatcaa tataatgata gaccgttaaa tattgaaaaa
     6901 gctttagacg ttattcagta caatgcacca ttacctaata ttttgcctga aagcgaaatt
     6961 attgaaaacc ataagtgtac acacattgta tcgaatgatt tctttacatt ggttaaatgg
     7021 gaaatttctg gcacgttaaa ttatatgaag cctagagagt tctgtttagt tacagtgttg
     7081 gaaggcgaag ggcaaatgat tgtcgatggt gaaattttca aactgactac tggtacaaac
     7141 tttattttga cttctgaaga tttggatagt gtctttgaag gtgatttcac attgatgatt
     7201 agctatgtgt aatagtgtaa taacaattta ggagtgaatg atttgaagca atttctatat
     7261 attgcgttag tatgtggtgt gatagcaggt cttggtgctt tcttacatat accgcagtat
     7321 ccgagcatga caattccacg tatagtagct attttaggaa ttatcagtgc tatgttgact
     7381 tttaaagaca agcaaatcag cgcctcatta aagtttagcg cattgttaat taatgtgctg
     7441 ccattatgcg gtacctttgt agcttcaaat taattgtcga tagcgcgttt gaatataata
     7501 cagtgcatta acagaagaaa tatgattgtg ttaatgacat tgatgaaaat aaagcacatc
     7561 aacataacct ctcacttgca tagtagtgag ggggtttttg ttggtttata taaaatgaag
     7621 taattaatat ttaatgaaaa ttaagacatg ataaaattct aacggcagta tagatattta
     7681 aaaatgtgta tcaatggcgc tatagagata agcaagcata gtggtatcat atataaattt
     7741 gtaattttta ttgcatagtg tatgcaagca tgttttttat atgcaaaata ggtagctaaa
     7801 tatgttaaac tgtttgtaac aatttttatc attaagacag atataataaa taaaatatag
     7861 aaaactgatt aaaaggtata cagttgctcg tatgtgtgtc caactgtatt tagagtcgca
     7921 cttgttgtaa agcaaaatag ttcgacttac ttagaagtga aaacgataag ggtctgggac
     7981 gtaaattaat gtcctgggcg ctacaatgtt ataggaaatg cgattaaaat cgcagtgaac
     8041 aaagggtata cagttgttcg catacgcgtc caactgcata agaaacacta aaagaatgta
     8101 attcatagtg tttctaaaat ttattcaggt taaggagtat taagatgaca gcagaaacga
     8161 attatttttg gttgaattgt ggatataacc gttggaatca taatgaaccg ttagtaggac
     8221 aaacagcttt atttgaatca ggcgcacatt ttaacccatc tcaaggattt agagcgttta
     8281 aaaaggctaa agtaggagat caagtcatct tttatcaagt tcagacggat actggactac
     8341 ttggatgtgg tgaaattatt agtgttgaaa caggtgcaca aaataaaata cgtgtacagt
     8401 ttcgttttaa tgaacaattg aaaccattaa cagcagatta tttaaagcgt agtgaagctt
     8461 tggaatttcg tataagtaat atgaaagaga cattgttcaa tcaaatcact gcagaagagt
     8521 ttgatttaat aagtggtctt ggtaaaggcg aaattaagat accgcgttat ttctttttag
     8581 ctgaaactga agaatttgaa cctggaaacc aatatacgat ttatacgcac acgtacaacg
     8641 gtattaaacg taatggttat catttttata ctcagttaga agaaggcgac aatattattt
     8701 tttataatcg aacgaaaaat caatctgtcg ttggaattgg agaagtatca aagcatattc
     8761 atgaaaaacc accaattcct ggtagaacaa atagtacagt gattgaagtg tcttatgaga
     8821 aagacattac accgattaca ttaagtacat taaataagca tcctaaattg aaaaacttat
     8881 atttccttca agaaaatgca aaacaggcta ttgcaagtat gtcacaagca cagtatgatg
     8941 caattattga aatgagcgat aataatggat tgaaatctcc atttgaaatg gttcaaaaac
     9001 cagatatgct tgaaagtgaa aaagaagaag cgttaaagcc atttatttta cttgttgtag
     9061 atcgaaaaga agaaggctta aaagcggcta atgacttatt gcaaaaagcg aatgcaaatc
     9121 ctgttataac aacaggtcat cctgattttt cagaagatat gctttatggc aagtatttac
     9181 caaatgaaac aggggcatta tactatcgtg aagggtttat tactcaatta atgcctaaaa
     9241 aggataaaag ttatttagtt attgataatt ttaatcgtat agatacagac attttccaaa
     9301 cgtatattaa tgtgttagaa ggctatgaag taacattacc acgttataat aaagacggta
     9361 atatgattaa atggtcacga caaaaagatt ctttctatta tttcaatcct aattggcata
     9421 ttgtaggtat tacatatgac agtttagagg aaattaagga gaaatattca gagcagtttt
     9481 taaaatacac acgtatagtc aaagtcaaac atgactaatg atatatggta gtttttgaaa
     9541 agaagtaagg ataggattaa aaattgcata attgcatatt tgaataaccg cataaagaaa
     9601 aaatgacggg taatcaacca taagagctaa ttaagagctt atgatagatt actcgttttt
     9661 attttaataa ataataaaga cagtatatga atataaaaga atttatgtta cagaattaaa
     9721 actttatatg agttttccag tggtatgttg aataagacga atgaccaaac gctaagttaa
     9781 taataagaaa taagattaca ctgtagattg agaaatttaa agtaaataaa aaatacatta
     9841 tacaataaat ataaaactga aaaataaatt ttatggcaat ttcgtgtcgc gaggacttat
     9901 caagtgacaa ccttgtttaa atgtttttaa attaggaaaa atgactataa gtactattaa
     9961 aggagcgaat aaattatgcc aatcgtatat tatgatggaa actgtatata ttgttataac
    10021 tatgtcattt ggttaattca acatgaccta ccaagaaatt atcaatttgc cacgttaaaa
    10081 ggtgaagttg gccaacaatt ttttaaacaa catccagaag cggcaaataa aaatagtgtg
    10141 attttacaga aaggtgatcg tatatatttt gaatcgcagg cgattattaa actaatcaca
    10201 gcactaccta atcgaactaa attattgggc gtaggtttat ggcttgtacc taaaccagtc
    10261 cgtaatttcg gttatcgaat gtttgctaat aatagaaata gaatgtggaa aacaacatgg
    10321 caccaaccaa atgattatga aaaatcgttc tttttagatg ataatgcgaa agtaaaactt
    10381 actgattgat aaaacatact tgctaattga taatggatat attagatgat gaattaaaat
    10441 ttaggcattt aaatagcgga acactttacg gttagattag aataattata aaaaagagag
    10501 caaaaacact ttacagatta gaattattat aatataataa ttaatataaa caagcaagac
    10561 gtagacaatt ttaaggagtg tattaaatat gagtaatcaa caagatgttg taaaagaatt
    10621 gaatcaacaa gtagcaaact ggacagtagc ttacacaaag ctacacaatt tccactggta
    10681 tgtgaaaggg cctaacttct tctcattaca cgttaaattt gaagaattat ataatgaagc
    10741 aagccaatat gtagacgaat tagctgaaag aattttagcg gtaggaggaa accctgtagg
    10801 tacattaact gaatgcttag agcaatcaat tgttaaagaa gctgcgaaag gttactctgc
    10861 agaacaaatg gttgaagaat tatcacaaga cttcacaaat atctcaaaac aattagaaaa
    10921 cgcaatcgaa attgctggta atgctggcga tgatgtatca gaagatatgt ttataggtat
    10981 gcaaacatca gtagataaac ataattggat gtttaaatct tacttaagct aatctacaga
    11041 taagtaataa tataaataaa agccaacaca taaatgggga tacgcccaga atgtgttggc
    11101 tttttgagtt taatcgcatt gttaaagagc aattaaagtc aatttaaata agttactctg
    11161 cgatttctaa agcgatttcc atcatttgtg taaatgaatt ttgacgttct tcaggtgtag
    11221 tagcttcgtc acgtaaaata tgatcactta ctgtgaaaat accaagtgct tttttaccag
    11281 catgaatcgc atttaaatat aaaccagctg attccatttc gatacctaaa atacccattt
    11341 ttttccaagc atcattgaat gttggatcgg cattgtaaaa tgtatcagaa gataatacgt
    11401 tacctacgtg tgtagtagca ccgatttggt cagcgacatt tttagcttta gttactaact
    11461 cgaagtcagc gataggcgcg aaatgacctg gaatattgta ttgatctaca taatttgaat
    11521 tagttgatgc agcttgtgca ataataacat cgtataagtt aacattttct tgtaatgcgc
    11581 cacaagaacc gatacgaatg attgtatcta catcaaagaa gttgtataac tcatatgagt
    11641 aaataccaat acttggaata cccataccag aacccattac agaaacttct ttacctttat
    11701 atgtaccagt gtaaccaaac atgttacgta catcgttaaa ttgttcaaca ttttctaaaa
    11761 aattatcagc aatatatttt gcacgtagcg gatcgccagg cattaatact gttttagcaa
    11821 tttttactcc atttggttga atatgtggtg taccttttgt catttgttat cttctcctta
    11881 atattcatta ataattttaa gataacattt gtttcccttt tttgcgaatt tttgtactat
    11941 agaattaaag aattataaaa atataatcct gattagcttg ttgtcaagtc atcgttcata
    12001 atgaagatat cacgttcaat tgtattgttt gtttatggga aatgaattaa tgtaatagta
    12061 tatgtatgcg gttacataaa aagcgaacat ctaacctgat atttaaatga acctgacgct
    12121 caatcaacta atttacaacc gtatttttat aatcaaccat aaaggaggag atagaaaatg
    12181 aatagtgcaa aattgat
//
LOCUS       DANRDI010000075        12146 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.075, whole genome shotgun sequence.
ACCESSION   DANRDI010000075 DANRDI010000000
VERSION     DANRDI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.075"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1227
                     /gene="eap"
                     /locus_tag="PZ377_001577"
                     /gene_synonym="map"
     CDS             <1..1227
                     /gene="eap"
                     /locus_tag="PZ377_001577"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HDM6358449.1"
                     /translation="SNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAKYT
                     VNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLN
                     GTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGG
                     KRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSDLK
                     FTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSNIS
                     QLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISYKY
                     LNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIK
                     KIDIDVKQYTKSKKNK"
     gene            1238..1528
                     /locus_tag="PZ377_001578"
     CDS             1238..1528
                     /locus_tag="PZ377_001578"
                     /inference="COORDINATES: protein motif:HMM:NF015598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDM6358450.1"
                     /translation="MKFPVTINKFENIVSNEFVFYNASKITINDLSIKLKSAMANDQG
                     ITKHDIGLAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIELKF"
     gene            complement(1852..3138)
                     /locus_tag="PZ377_001579"
     CDS             complement(1852..3138)
                     /locus_tag="PZ377_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDM6358451.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            3338..3436
                     /locus_tag="PZ377_001580"
     CDS             3338..3436
                     /locus_tag="PZ377_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358452.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            3679..3855
                     /locus_tag="PZ377_001581"
     CDS             3679..3855
                     /locus_tag="PZ377_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358453.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            4119..4499
                     /locus_tag="PZ377_001582"
     CDS             4119..4499
                     /locus_tag="PZ377_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDM6358454.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            4496..5392
                     /gene="pmtA"
                     /locus_tag="PZ377_001583"
     CDS             4496..5392
                     /gene="pmtA"
                     /locus_tag="PZ377_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDM6358455.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            5393..6073
                     /gene="pmtB"
                     /locus_tag="PZ377_001584"
     CDS             5393..6073
                     /gene="pmtB"
                     /locus_tag="PZ377_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDM6358456.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            6070..6942
                     /gene="pmtC"
                     /locus_tag="PZ377_001585"
     CDS             6070..6942
                     /gene="pmtC"
                     /locus_tag="PZ377_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDM6358457.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            6942..7682
                     /gene="pmtD"
                     /locus_tag="PZ377_001586"
     CDS             6942..7682
                     /gene="pmtD"
                     /locus_tag="PZ377_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDM6358458.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(7748..8311)
                     /locus_tag="PZ377_001587"
     CDS             complement(7748..8311)
                     /locus_tag="PZ377_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDM6358459.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            8432..8956
                     /locus_tag="PZ377_001588"
     CDS             8432..8956
                     /locus_tag="PZ377_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358460.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            9016..9573
                     /locus_tag="PZ377_001589"
     CDS             9016..9573
                     /locus_tag="PZ377_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDM6358461.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            9570..10412
                     /locus_tag="PZ377_001590"
     CDS             9570..10412
                     /locus_tag="PZ377_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDM6358462.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(10469..11509)
                     /locus_tag="PZ377_001591"
     CDS             complement(10469..11509)
                     /locus_tag="PZ377_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDM6358463.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            11960..12133
                     /locus_tag="PZ377_001592"
     CDS             11960..12133
                     /locus_tag="PZ377_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358464.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         4317 a         1559 c         2021 g         4249 t
ORIGIN      
        1 tcaaacttaa catttaataa gaatcaaaat attagttaca aagatttaga ggataaagtt
       61 aaatcagttt tagaatcaaa tagaggtatt accgatgttg atttaagatt atcgaagcaa
      121 gcgaaatata cagttaattt taaaaatgga acgaagaaag ttatcgattt gaaatcaggt
      181 atttacacag cgaatttaat aaattcaagt gatattaaaa gtatcaatat taacgtagat
      241 acaaaaaaac atatcgaaaa taaagctaaa agaaactatc aagttccata ttcaattaat
      301 ttaaatggta catctacaaa cattttatcg aatctttcat tttcaaataa accttggaca
      361 aattacaaaa atttaactag tcaaataaaa tcagtactga agcatgatag aggtattagt
      421 gaacaagatt taaaatatgc taagaaagct tattatactg tttattttaa aaatggtggt
      481 aaaagaatct tacagttgaa ttcaaaaaat tacacagcaa acttagttca tgcgaaagat
      541 gttaagagaa ttgaaattac tgttaaaaca ggaactaaag cgaaagcaga cagatatgta
      601 ccatacacaa ttgcagtaaa tggcacatca acaccaattt tatcagattt aaaatttaca
      661 ggtgacccac gtgtaggcta caaagatatc tctaaaaaag ttaaatcagt attgaagcat
      721 gatagaggta tcggggaacg tgaattaaaa tatgcaaaaa aagcaactta cacagtacat
      781 tttaaaaatg gaacgaaaaa agtgattaac ataaattcaa atattagcca actgaatctg
      841 ctttatgttc aagatattaa aaagatagat attgatgtta aaacaggaac taaagcgaaa
      901 gcggatagct atgtaccata tacaattgca gtaaatggca catcaacacc aattttatca
      961 aaacttaaaa tttcgaataa acaattaatt agttacaaat atttaaatga caaagtgaaa
     1021 tctgtattaa aaagtgaaag aggcatcagt gatcttgact taaaatttgc gaaacaagca
     1081 aaatatacag tatatttcaa aaatggaaag aaacaagtag tgaatttaaa atcagacatc
     1141 tttacaccta atttatttag tgccaaagat attaaaaaga ttgatattga tgtaaaacaa
     1201 tacactaaat caaaaaaaaa taaataaatc taataatgtg aaattcccag taacaataaa
     1261 taaatttgaa aacatagttt caaatgaatt tgtgttctat aatgcaagca aaattacaat
     1321 taatgattta agtataaaac ttaaatcagc aatggcaaat gatcaaggga taactaaaca
     1381 tgacatagga cttgctgaac gcgcagtgta taaagtgtat tttaaaaatg gttcgtcaaa
     1441 atatgtagac ttaaaaactg agtataaaga tgaaagagta tttaaagcaa ctgacattaa
     1501 aaaggtagat attgaactta aattctaatc tttaatttta taataaggca tctcacaatc
     1561 gtggggtgcc ttttacattt gtagagatgt gatgcttgaa tgtttgaagt gatttgccaa
     1621 aagttatata aattggttct ataataaatc ggactgatag aaaaagtttt ttacttttca
     1681 tctgtccgat tttttgattt tgttaggaga gatgtataaa actaattagg tatagtgttt
     1741 gacaagttgc tgaataaaat atatcttatg atactttgaa agaaggaata attttaaaaa
     1801 taaaaaacca ataatccgag tcatactcat cagattattg gttgaaatta attatcttaa
     1861 gtcatcaatc gctttagcaa ttgaatcaaa tacatgtggg atatcttctt tttctacaca
     1921 gctaaacgca atacgaatat ctgttgcatt aagtgcaata acaccaattg aatatttttc
     1981 aattaaatgt ttacgaagtt cttcagggtc aacatcatga acttttattg ccataaagta
     2041 tccagagtta aagtcatagg cttgccaatg tgaatgatat tgatcagcat agacaacttc
     2101 cttagtgact tcatagcgtt cttttaatgt ttgaatattt tgttcgattt ctttatcgaa
     2161 ttgtttatta ttttttaata catgcttaac agcgctttgt gttggaagag gtccactaga
     2221 aatgttactt cgtataagac cttttacttt ggcttctaat acttctttag ttgtttgatc
     2281 agacgttcca aatgtcataa acccaacacg gaatccccat gcgaaaaatt cttttgttgc
     2341 accatctaaa cgaacaggta atattgcatt tgaatttaaa ttagataatg cagtaaataa
     2401 tgattgagta tacacatctt catagaataa accatagtat gcgtcatcca caacagctat
     2461 aacttttgta cctttatcag ctaatgcttt aattgcatcg acgatggtag tcacttcttt
     2521 atgcgtaggt gtgtaacctg tcggattatt aggataattt aaaatcataa tgactttatc
     2581 tttattgtat gattgtaaag cttctacaag tgaatcagta gtataatgcc cgtctttatc
     2641 aaagatagga tatgtttgaa ggtttgcacc atttctcgta ttgaaaacaa gtttgtaatt
     2701 accccaatta tgctctggta acaagatagt gtcaccttga tttacaaata aatcgccaac
     2761 taaagataaa ccatgtgtta atgcattcgt aacaattggt agtgacatgt tgtcgattga
     2821 tagctctgga ttgtcacgca acattttttg ttgccataaa tcacgtaatt cttcgatgcc
     2881 ttgtggtggc gcataaggga atatttcatc tggagttaaa tcattaaaca ttgcatctaa
     2941 agatgatgca aacatttttc cgtctttgtt tgtcgccata cctatagttg cattatatgt
     3001 tgtactcttt gcttcagcag attgagataa aatgcctttt ggataaaaca tattttgacc
     3061 taagtcagat aacattgtaa aggcagttgc atttgactgt tgaagttgtt catttaagct
     3121 ttgggctaaa ggattcatta ttgcttaacc tctctttaaa aaaattgtat gcctatatat
     3181 tatcttcaat tataccaaag ataaagtgtt gttggtgaat taacgaataa tatgtaaaat
     3241 ctgctgccaa aatacgaaca tatgttctgt ttttatgtta gaataggtgt aggtcatcct
     3301 tcagtatgat actttagtcg aacggaggtg tagtgtaatg atgtggtggc aagaagggat
     3361 gatgacactt attacaggtg gcttactaat tatttttcga ctttggttag aactgaaatg
     3421 gaaaaataaa aaatgatcct acacacataa ataaccccaa gtctgctggt accagacttg
     3481 gggttgtgta gtataattct gtggatacac ttaaataata tcattgaaca caatatatcg
     3541 caatcaataa tcattttgat tgaatggttt tgttgcgcta ttttagttga aagtgattag
     3601 taccttgaac ttttgaaatt caataactta ttaaaaaaat ttatatttgt tataatgtcg
     3661 atgttgaggt gaaataaatt gaaacatcta acaaaaatat ttgtagtact tgcaattatt
     3721 ttatttatta ttggttatta tttacaagca actaatcatg aaagccaagg tataaaatta
     3781 ttattagcag cgattatgtt tatgatttgt gcttttataa gtagaagtaa tgatcgacgt
     3841 aaaaatagta aatagaattg actgtttaaa acgattgttt atgttgacgc tacggttgtc
     3901 acgtagagat taaagacatt cgttttagca gtctttttta ttgaagtaga caatgcaaga
     3961 ttaaatggta gtgtcatttc attttatttt attcaactgc tagtaataca atactcagtg
     4021 ttatttctga agactggttg ataatgagac atgcatattt acaaaactgt gtatattgtg
     4081 tatattgtat atatacagaa gttgataggg ggacgttgat gaaaataatt ttaaaaaaca
     4141 atagtgattt tccgatttat gaacagatta agcaacaagt aaaacaaaat attttaaagg
     4201 gacatgttgc tcctggagag catttgccgt caatgagaga acttgccaaa gatcttcaag
     4261 taagtttgat tactaccaaa cgtgcttatg aagatttaga gaaagacggt tttgttacaa
     4321 caattagagg aaaagggacc tttgttaagg agcaagatag ttctatttta aaagagaaac
     4381 aattttttac cattgaaaat ttggttaaag aattggttaa tgaagcgcaa gccatcgaaa
     4441 tgtcacttga ggaactgcag gatattttaa cgttcattta tgaggaggaa tcatcatgaa
     4501 tgccatagaa ttaagtaatg ttaattattc aagtgatcag tttaatttaa agaatatctc
     4561 tttcaaagtt cctcaaggat tcgtcactgg ttttattggt agaaatggcg ctggtaaaac
     4621 cacaataata aggttaatta tggatttgta tcaaccacaa actggtgtta ttagagtatt
     4681 agaagaggat atggctctca atccgataga gttaaagaat agaattgggt ttgtttactc
     4741 tgaaaattat tttaatgaga gatggactac gaagcaactt gaaaaaatga ttgccccttt
     4801 ttatcgtaaa tgggaccatc aagtatttga attttatctt gaaaagttcg atttaccaat
     4861 caataaatca ataaaaacat tttcgactgg gatgaaaatg aaattatcac tagcagttgc
     4921 attttcacat catgctgaat tgtatatttt tgatgagcct acttcaggtc ttgatccctt
     4981 agctagaaat gagctattgg agataattca gcaagaatta attgatgaaa ataaaacaat
     5041 attcatgtcg acacacatta tctcagattt agaaaaaata gctgattata tcattcactt
     5101 aagtgacggt gaagttatct taaatggttc aaaagaacaa ctgttacaaa gatatcaagt
     5161 tgtgagtggt gctattgagg accttgatga tgaattagca tctctactta tttatgaaga
     5221 acataagcgt acgggattta taggattaac agaacatgcg caagtattta aagaaatttt
     5281 aggtcataaa gtgaatatca ctacaccttc tattgagaac ttaatggttt atcttgaaaa
     5341 gagaaaacct aaatatcatg aaaacatcaa attgatggaa gaaggttttt aaatgaaggc
     5401 attgttaatt aggaatttta aattacgacg atatacactt atcatctatg ttctattatt
     5461 aaccttatat cctttttata taatgcttga ctcaacaaag ttcttttact tattgcaatc
     5521 ttttataagt ccaacaatat tgattatttg gattttagat gctggtcact tatttagact
     5581 caatcgtaga ttaggtggta atgattcata ttatttttat atgagtttac cagtgtcaaa
     5641 aaaacaattg cttaatgcca actatattac atgcattgtt ttaacattaa ttggaacgct
     5701 tgttattagt ttatatgctt atgaagcaga tgtgattgaa ccaaattcaa tctatttttc
     5761 aactgcatat gcatttgtca tatctaactt cttgtctata ccaattgcat ttagtcaatt
     5821 tacagaattg cgtagagtca aagtgccata tggtatatac gtgtttacta ttatcatttt
     5881 agttccattt ttattttcaa ttgcaatagt attggtgaat tattttgttc taagtcaatc
     5941 atcattccca gatttatatt catatatttt aaatattggt tttctaatta taagcattgt
     6001 tatacttatt gttaattatt ttaaacagct caataaaata aatactagaa aatttaaagg
     6061 aggcagtcga tgaaattaga acatattaca aaaaaatacg gctcaaatgt cgttttaaat
     6121 gatattgatt ttgactttgg cgatagtaga attgtcggat taataggaaa aaacggtgtt
     6181 ggtaaaacaa ccgttatgaa agtaatgaat ggtaatatta ttaaatttga tggaaaagta
     6241 gatattgata atgcagataa tatcggtttt ttaattgagc atcctaaatt atatgataat
     6301 aaatcaggat tgtataactt gaaattattt gcacaagtat taggtaaggg ttttgataaa
     6361 gcatacacag acaaaattat agatgcattt ggtatgagac cttatattaa aaagaaagtt
     6421 aagaaatatt caatggggat gaagcaaaag ttagcaattg cagtatcttt aatgaataaa
     6481 cctaaatttt taatcttgga tgagcctaca aatggtatgg atccagatgg ctcaattgat
     6541 gtgctgacta caattaagtc tttagtaaat gaacttgata tgagaattct aatatcaagt
     6601 cataagttag aagatattga attaatttgt gatagagctg tatttttaag agacggacat
     6661 tttgttcaag atgtaaacat ggaggaaggt gttgcatctg acacaacgat agttactgtt
     6721 gatcataaag actttgatag aactgaaaaa tatcttgcag agcatttcca attacaaaat
     6781 gtcgacaaag cagacggaca tttaatgatc aatgcacaaa aaaattatca agttatacta
     6841 aaagcattat ctgaattaga tatttatccg aaatatattg aaacacgtaa aagttcattg
     6901 cgtgatacgt acttcaatat aaatcaaaga ggtgataaat aatgagaatt ttaaatttag
     6961 ttaagtatga tttttatagt atatttaaaa gtcctttaac atatttagcg atactagtcg
     7021 tatctagttt gattgcaact caaagtatac ttatggcaaa ttcgatggat aacccgaaac
     7081 atattattgt ctatggatct gtatttgctg cagcaaaatg gttattgtta ataattggat
     7141 taatgtttgt tgttaagaca attacgcgtg atttttcaca aggtacaatt caactatata
     7201 tgagtaaagt taaaacacgc gttggataca ttatttcgaa aacaatttca attattttaa
     7261 tttcaatatt atttgcatta attcattatg tgattttgat tgttgtgcag gcatctagta
     7321 atggaaaaaa tttggcgttt tctaaatatg tagataattt atggttcttc ctaatctttt
     7381 tactattctt tggcttgttt ttattcttaa tcacacttgc atcacaaaaa acagcaatga
     7441 tattttcatt aggtgtattt ttagtactca ttgtaccgtt tattaaacct tttattacat
     7501 ttatcccaag atacggtgaa aaagttttag atgcttttga ttatatccct tttgcttact
     7561 taactgataa aatgattagc tctaactttg attttagcaa ttggcaatgg gtaatttcat
     7621 taggttctat agtgatattc ttcattttga atatcttata tgtcgctaaa aaagacattt
     7681 aataaaaata attttgaggt tgggaatttt aaattttccc aacctcaaag tttgtcttat
     7741 tgtaaattta tttattttct aatttattta ggatggaatt ataaactgct ttccaaaatg
     7801 aagcgtcagt tttatagcgg tttgatataa ctaagtgtgt ttctttttct aaatctgcat
     7861 agtctggatg atctttgctc ggtaatttat cagcacgaac atcagttaca aatttttgga
     7921 cttcatttgc tcttggtccc caaactgttt cttgttcgaa ttgatcattc aagaatacga
     7981 agataggaat tgcacgtgat ttaccatttg ttaaatattg atcgatcagt tttgtatcat
     8041 catctctatg gaacacgcgt acttctaaat ttaatgcttc actgatgtgt tttagaattg
     8101 ggagattcat cattgcatct ccacaccagt cttcagtaat tactaatact ttagaataat
     8161 tcatctcttt tatttttttg atgcgtgaat catcttctgg taactcaaat gattgataga
     8221 tactgagaac ggtatcttga tttgtcttca ttccatcaat gtattcattt aagggttggc
     8281 tatttttgaa ataagtttct aaatttgtca ttgtaaaaac ctcctttagc atttacaaca
     8341 ttataccaat ttatagtaat aaaaggtaat gaaataaatt aattgcaaat tctttgttaa
     8401 tttttgttaa ggatgaaacg ggaagcacct tatgctatat ttaaataagt acaaagaaag
     8461 ggtgacatca gtgcgtattc aaaatcgctg ggttgtgttt atattatttt taatctgttc
     8521 ttttggtgta ttaattggtt tatatcaata tcgtcatacg aaaactgtgg atttgtctaa
     8581 tcttgaaata aacgatatta aattaaatga gaagtttgat aaaaaaggct atgaagtaaa
     8641 taaaaaaatt aagtttgatc gttttaaatt ttataacagt aaagctcacc ctgatcttac
     8701 cgttaaagtg agagaaaagg ataacatcgt taaggggata atattagtaa gagatgaaaa
     8761 gatacatact aattttgatg ggggaattgg ttcgccgata aataacgcga ttgaaaatct
     8821 tggattcgga tataaaagaa caaaagttgg caatgatttc tcatcggtaa agtatattga
     8881 tagagataac catttaaaat taaacttact ttatcaagat ttagaaatta aacgtattga
     8941 attttttagt aaatagcttt aggtcttaaa gttataaaaa acgaatgaat aattttattg
     9001 ggatgagtga cctatatgaa caagattact tatttaaatg aattagaaac agcacttgat
     9061 gcgctaccta ggcatttaag agatcaaaaa atgtatgaat atgaacgtta tttttatgag
     9121 caagaactta atggcgttga tgaaggggaa attttaaaga agttaaaaga cccacaagat
     9181 gttgcagctg aaacaaaagc tagaagtgtc attgattatg ctgaatctaa accaacattt
     9241 gaaaatattt caagagctgt tgctgcttca ttaagtttag gcattctatc tatttttgtc
     9301 atccttatac cagtatctat agttggatta tttgtattag cattattttt aatatcactt
     9361 ttgctgctgt tttgtccaat tattttatta gcatcagcaa tatccagagg aattgtggac
     9421 tcaattagta atgtattttt tgccatatca tattcaggat taggattagt atttatcatt
     9481 gtcatattta agattttaga atacatttat cgtttaatct taaaatattt actttggtat
     9541 attaaaactg tcaaaggaag cgttagaaaa tgaagaaatt cttttttatt gggcttttag
     9601 tgtttgttgt cttttttaca gcagcaacca ttatttggtt cagctatgat aaaaacaaat
     9661 atggtactaa acaatatgat aaaacattca aagacgatgc ttttgacaat gtatctataa
     9721 atttggatag tacagaactt cgtataaaac gggggaatca atttagagtt aaatatgatg
     9781 gtgacaatga tatattaatt aatatagtag ataagacgtt gaagattagt gataaaaggt
     9841 ctaagacaag aggatatgca attgatatga atccttttca tgagaataag aaaacgttaa
     9901 cgattgaaat gcctgataaa atgattaaac gtttaaatct atcatctgga gcaggaagtg
     9961 ttagaatcag tgatgttgat ttagagaaca caagtattca aagcattaac ggtgaagtag
    10021 ttattaaaaa ctcaaatctt gacgcactag attcaaagac aaataatagt tctacttata
    10081 ttagtaaaag taacattaaa aatagcaata ttaaagttgt tattggtacg ctacaaatcg
    10141 acaagagtca aattaaacaa tccatatttt taaacgatca tggtgacatt gaatttaaaa
    10201 acatgccatc aaaagtagat gcaaaagctt ctactaaaca aggagatatt cgttttaagt
    10261 atgatagtaa acctgaagac actatactaa agctaaatcc gggaacgggt gatagcgtag
    10321 ttaaaaataa aacatttact aatggtaaag ttgggaaaag cgacaatgtt ttagaatttt
    10381 atacgattga tggtaatatc aaagttgaat aaataaagga tgtaagcacc gatattagga
    10441 agcataattt ctctaatatc ggtgttattt atttgttggc aaaagttaag tcggtatcta
    10501 tattgccagt aaagtgagtg atattaaggt cttgaccatc taaccatgat ttgaaatcta
    10561 ttatttctgg tggcgcattt tctcccaatg taaaatatgc agttaatgtt tcaggttgat
    10621 acattgatgt atggatggtg ccagaccagc ttttgaatag tttactgtaa atttcatact
    10681 gaggattatt gaataactta aatgctgtag tcatatctaa attatcatta gtttgtgaaa
    10741 tggtacgcgc cagtctttct ttagattctt ttgtataatt acgattttca tgtgttaata
    10801 tttcaaaatg atttgtacat atattatcat aacgaacatc tattgatctc ggtgtcactt
    10861 caacaattgc atggttcaat gatttgtcca tcagtatgta gctaaatgag cttctgtgtg
    10921 gtatttcttt caataattgg attgcttctg ttacatttcg gcaattttca agaattagac
    10981 gaccaatcat ataacataca aaaccatttg ctggtttctt ccggtgcata aagttatagc
    11041 ccatagttaa tcctgactca ttcataccat ccattcttcc agttaccctt gatacaggac
    11101 caatttgagc taaaccgcta tctgtaggtt gataaagtaa gtagcgacca tcataagttg
    11161 cagggtggta atcataattt ctaaccatga agtctttgcc ttgaaagacc gtgcaaccac
    11221 tttcttttaa atcggtaaaa cgataatgtc caaagtttaa aataatttgg cgtgttggca
    11281 ttttgagtat actttgtagt cccattaatt cttcccatat ttgaggtgcg tatgtttgga
    11341 atatttgata agtttcattt acatctatat cgaaacgtgg gacacgtttt ttccattctt
    11401 tttctcgatt ttttagaaga ggtgtttgtt gaagccattt accagtttta acacctaact
    11461 cgaaatgtga acctctaaaa gtcatgatat ctgatgtcac ttgttgcata tcatcggccc
    11521 ctttcttttt agttgtaata tattgtaaat aaatagtaat cgtatgtata ttgaatgtca
    11581 tgttaaataa agttatattt tactaaatga aatataaaat tgtttgaggt gatttctcgg
    11641 tgtataagac ttatcaatca gttaaaacat atttttatag atggtgggga tattgagtta
    11701 aaaacttaaa atcatcttat cataaatatc aatcttaagt tagcattcac gataatagtc
    11761 attgttaaca ttagcatata aggtcatgtc acgttgaaac agaggttcct cggcattttt
    11821 gaaaataatc attagcttgc tcactatata atttgatgaa tatatttcgt gaaagtgggt
    11881 atttatttaa tgattattct atatatgata gtataatgaa atgtagatag gtatttaatt
    11941 taacagaggt gaaattgaga tgtggaattt tattaaatgt gtgtttaaat tcgtatttag
    12001 cttagttgct attacaacat tagttgctgg tgttggtgta gtagcatttg cttatatctt
    12061 taaaaaagat tttgaagata ttgaaagaaa aactaaagaa attatttctg atattgaaag
    12121 taaaaataac taataacatt tagagg
//
LOCUS       DANRDI010000076        12139 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.076, whole genome shotgun sequence.
ACCESSION   DANRDI010000076 DANRDI010000000
VERSION     DANRDI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12139
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.076"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            108..308
                     /locus_tag="PZ377_001593"
     CDS             108..308
                     /locus_tag="PZ377_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358465.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            385..516
                     /locus_tag="PZ377_001594"
     CDS             385..516
                     /locus_tag="PZ377_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358466.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
     gene            778..1437
                     /gene="arlR"
                     /locus_tag="PZ377_001595"
     CDS             778..1437
                     /gene="arlR"
                     /locus_tag="PZ377_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDM6358467.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1434..2789
                     /gene="arlS"
                     /locus_tag="PZ377_001596"
     CDS             1434..2789
                     /gene="arlS"
                     /locus_tag="PZ377_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDM6358468.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            3073..5871
                     /locus_tag="PZ377_001597"
     CDS             3073..5871
                     /locus_tag="PZ377_001597"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDM6358469.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5885..7153
                     /gene="sucB"
                     /locus_tag="PZ377_001598"
     CDS             5885..7153
                     /gene="sucB"
                     /locus_tag="PZ377_001598"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDM6358470.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            7733..8542
                     /locus_tag="PZ377_001599"
     CDS             7733..8542
                     /locus_tag="PZ377_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6358471.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            8571..8774
                     /locus_tag="PZ377_001600"
     CDS             8571..8774
                     /locus_tag="PZ377_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358472.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            8955..9746
                     /locus_tag="PZ377_001601"
     CDS             8955..9746
                     /locus_tag="PZ377_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDM6358473.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            9760..11646
                     /locus_tag="PZ377_001602"
     CDS             9760..11646
                     /locus_tag="PZ377_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HDM6358474.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            11871..>12139
                     /locus_tag="PZ377_001603"
     CDS             11871..>12139
                     /locus_tag="PZ377_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDM6358475.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGP"
BASE COUNT         4550 a         1708 c         2164 g         3717 t
ORIGIN      
        1 aaacaaaagc agtaaaccta aagtgtcgta agggtttact gcttttataa aacgttgtta
       61 taacgtatat tgtcttttac gggcatataa aaggggaata tttgaaaatg accaatccaa
      121 caagaacgaa acgttgtggg ggggatgttc tatatggtat tgataatcat tttcaactac
      181 tattatacat tagtgagaat cattgtcaat tagaaactaa aacttttttt gaatattttt
      241 taagaatagt aaataaaacg catgattacg ctattttaga aaataaaaaa atttgtattt
      301 ctcattagaa ttagaatatt taaaagtgat gaggtttaaa cattatattg tttacatact
      361 ccttttgaat tcatacatta tgaaatgtta cttccaagtt caaaatcgca cattgaaatg
      421 atgtgtgaaa tgtttaaact acggtcattt tgtgaaaata aagataataa ctattcattt
      481 tacaatagtg aaaagtcagt atatgacaac aattaatatt gcggtaaggc cttgtgttac
      541 agtattctat atttaagtac tgcaatcaga attaacagaa tgccattaac tgattattaa
      601 atatttgagt taataaataa ttaatgattg tagcttgaaa aatttaaaac atggttattg
      661 atttgtgata aaatttaaac gtaaacaaac taatttaaaa agcaactatt gtatagaaaa
      721 atacaaaatt taaaatatat taccttatta gaaaaagtcg taatatgagg tgtacaaatg
      781 acgcaaattt taatagtaga agatgaacaa aacttagcaa gatttcttga attggaactc
      841 acacatgaaa attacaatgt ggacacagag tatgatggac aagacggttt agataaagcg
      901 cttagccatt actatgattt aatcatatta gatttaatgt tgccgtcaat taatggctta
      961 gaaatttgtc gcaaaattag acaacaacaa tctacaccta tcattataat tacagcgaaa
     1021 agtgatacgt atgacaaagt tgctgggctt gattacggtg cagacgatta tatagttaag
     1081 ccgtttgata ttgaagaact tttagcaaga attcgtgcaa ttttacgtcg tcagccacaa
     1141 aaggatatta tcgatgtcaa cggtattaca attgataaga acgcttttaa agtgacggta
     1201 aatggcgcag aaattgaatt aacaaaaaca gagtatgatt tactatatct tctagctgaa
     1261 aataaaaacc atgttatgca acgggaacaa attttaaatc atgtatgggg ttataatagt
     1321 gaagtagaaa caaatgtcgt agatgtttat ataagatatt tacgaaacaa gttaaaacca
     1381 tacgatcgtg acaaaatgat tgaaacagtt cgtggcgttg ggtatgtgat acgatgacaa
     1441 aacgtaaatt gcgcaataac tggattattg ttaccacgat gattacgttt gtcacgatat
     1501 ttttgttttg tttaattatt atttttttct tgaaagatac actgcataat agtgagcttg
     1561 atgatgcaga acgaagctca agcgatatta ataatttatt tcattctaag cctgttaaag
     1621 atatatctgc attagacttg aatgcatctt taggtaattt tcaagagata attatttatg
     1681 atgagcataa taataaatta tttgagacat cgaatgataa cacagtgaga gttgaaccag
     1741 gttatgaaca ccgttatttt gaccgcgtaa taaaaaaacg ctataaaggc attgaatatt
     1801 taattattaa agaaccaatt acaacgcaag atttcaaagg gtatagcttg ttaattcatt
     1861 cactagaaaa ttatgataac atcgtaaaat cattgtatat cattgcgctg gcatttggag
     1921 tgattgcaac aattataact gccacaatca gttatgtatt ttcaacacaa attactaaac
     1981 cgcttgtcag tttatcaaat aaaatgattg agattcgacg agatggtttt caaaataaat
     2041 tgcaattaaa tacaaattat gaagaaatag ataatttagc aaatacgttt aatgagatga
     2101 tgagccaaat tgaagaatca tttaatcaac aaagacaatt tgttgaagat gcgtcacatg
     2161 aattacgaac accattacaa attattcaag gtcatttaaa tttgattcag cgatggggaa
     2221 aaaaagaccc agcagtatta gaagaatcgt taaatatttc tattgaagaa atgaatcgta
     2281 tcataaaatt agtcgaagaa ttacttgaat tgactaaagg agatgtaaat gacatttctt
     2341 ctgaagcaca gaccgtgcat attaatgatg aaattcgctc gcgaatacac tcattaaaac
     2401 aattgcatcc tgattatcaa tttgatacgg atctgacatc taaaaatcta gaaattaaaa
     2461 tgaaacctca tcaattcgaa caattatttt taatctttat tgataatgca atcaaatatg
     2521 atgtgaagaa taagaaaatt aaagttaaga caaggttaaa aaataagcaa aaaataattg
     2581 aaattacaga tcatggaatt ggtattccag aggaagatca agatttcatt tttgatcgct
     2641 tttatcgagt ggataaatct cgttcaagaa gtcaaggcgg taatggactc ggattatcta
     2701 ttgctcaaaa aatcattcaa ttaaacggag gatcgattaa aattaaaagt gaaattaaca
     2761 aaggaacaac gtttaaaatc atattttaat catgactgag acgtcaatca aagtcatagg
     2821 atcaattttt taagtacaca ttagctgtga ctaatgtata agaacaacta taaaacaaat
     2881 aaacagtggt tctttatcat ttctgttgta ctcccaaaat ttacaataaa atacatctat
     2941 aaacctagaa gaatcaacgc ttttgttgat tcttcttttt agcagataaa taggtaaatc
     3001 tactttaaca aataactaaa tagtgatatt attacattgt aagcgtttca acatttttgt
     3061 ggagggtgta aaatgactaa cgaaagaaaa gaagtttcag aggctcctgt aaacttcggt
     3121 gcgaatttag gtctaatgtt agatctatat gatgactttt tacaagatcc atcatctgta
     3181 ccagaagatt tacaagtctt attcagcaca attaagaatg atgactcaat tgtaccagct
     3241 ttaaaaagta caagtagtca aaatagcgac ggcacaatta agcgtgtcat gcgtttaatt
     3301 gataatattc gccaatacgg gcatcttaaa gccgatattt atcctgtaaa tcctccaaaa
     3361 aggaaacatg tacctaaatt agagattgaa gactttgatt tagatcaaca gactttggaa
     3421 ggtatatcag caggaattgt ttcagatcac tttgccgaca tttatgataa tgcttatgaa
     3481 gcaattttaa gaatggaaaa acgttacaaa ggaccaattg catttgagta tacacatatt
     3541 aataacaata ccgaacgtgg ttggttaaaa agaagaattg aaacgccata taaagtaacg
     3601 ttaaataata acgaaaaaag ggcactattc aaacaattag cgtatgttga agggtttgaa
     3661 aaatatcttc ataaaaactt cgttggtgca aagcgttttt caattgaagg ggtagacgca
     3721 cttgtaccga tgttacaacg tactattacg attgctgcga aagaaggtat taaaaatata
     3781 caaataggca tggctcaccg tggacgttta aacgttttaa cgcatgtctt agaaaaaccg
     3841 tacgaaatga tgatttcaga atttatgcat acagatccaa tgaaattctt acctgaagat
     3901 ggtagcttgc agttaactgc tggatggact ggtgatgtga aatatcacct tggtggcatt
     3961 aaaactactg attcatacgg tacaatgcag cgtattgcac tggctaacaa tccaagtcac
     4021 ttggaaattg ttgcacctgt tgttgagggg cgtacgagag cagcacaaga tgatacacaa
     4081 cgagctgggg ctccgacgac tgatcatcat aaagcaatgc caattattat acatggcgat
     4141 gctgcttatc ctggtcaagg aattaacttc gaaacaatga acttaggaaa cttgaaaggc
     4201 tattctacgg gtggttcatt gcatattatt actaacaata gaattggatt tactacagaa
     4261 ccaattgatg cacgttcaac aacttattct acagatgtgg ccaaaggtta tgatgtgcca
     4321 atattccatg tcaatgcaga tgacgttgaa gctactattg aagcaattga tattgcaatg
     4381 gaatttagaa aagagtttca taaagacgtc gttattgatt tagtaggtta tcgtcgtttc
     4441 ggacataacg aaatggatga accatcaatt actaatccag ttccttatca gaatattcgc
     4501 aaacatgact ctgttgaata tgtgtttggt aaaaagcttg ttaatgaagg tgtcatttca
     4561 gaagatgaaa tgcattcatt tatagaacaa gtccaaaagg aactaagaca agctcatgat
     4621 aaaattaata aagctgataa aatggataat ccagatatgg aaaagcctgc agatcttgca
     4681 ttaccgttac aagcagacga acaatcattt acttttgatc acttgaaaga aataaatgat
     4741 gcattgttaa catatccgga tggctttaac attttgaaaa agttaaacaa agttcttgag
     4801 aagcgtcatg agccgtttaa taaagaagat ggtttagttg attgggcaca agcagaacaa
     4861 cttgcatttg cgacaatttt acaagatggt acaccgattc gcttaactgg tcaagatagt
     4921 gaacgtggta cattcagtca taggcatgcc gtgttacatg atgagcaaac aggtgaaaca
     4981 tatacacctt tacatcatgt tcctgatcaa aaagcgacat ttgatataca caattctccg
     5041 ctttcagaag cagcagtagt tggttttgaa tacggctata atgtggaaaa caaaaaaagc
     5101 ttcaatattt gggaagcaca atatggtgat tttgcaaata tgtcacaaat gatttttgac
     5161 aacttcttat tcagttctcg ctcaaaatgg ggagaacgtt caggattaac attattctta
     5221 cctcatgcat atgagggtca agggcctgaa cattcatcag caagattaga gcgattttta
     5281 caattagctg ctgaaaataa ttgcacagtt gtcaacttat ctagttcaag taattatttc
     5341 cacttattgc gtgcacaagc ggctagttta gattctgaac aaatgcgacc attggttgtt
     5401 atgtcaccaa aaagcttact gagaaataaa acagttgcaa aaccaattga tgaatttact
     5461 tctggtggat ttgagccaat tttgacagaa tcatatcaag cggataaggt tacaaaagtt
     5521 attttggcaa ctggtaaaat gttcattgat ttaaaagaag cattagctaa aaatccagac
     5581 gaatcagtat tactcgttgc gattgaaaga ttgtatccat tcccagagga agagattgaa
     5641 gcattactag cacaattgcc aaaccttgaa gaagtgtcat gggtacaaga agaacctaaa
     5701 aatcaaggtg catggttata tgtctatcca tatgttaaag tgctagttgc agataaatat
     5761 gatttaagtt atcatggcag aattcaaagg gctgctccag ctgaaggcga tggagaaatt
     5821 cataaacttg ttcaaaataa aattatagaa aatgcattaa aaaataacta gggggaaata
     5881 agtcatgcca gaggttaaag ttccagaatt agcagaatct attacagaag gtaccattgc
     5941 agaatggttg aaaaacgtag gggatagcgt agaaaaaggt gaagctattc ttgaattaga
     6001 aactgataaa gttaatgtcg aagttgtatc tgaagaagca ggtgtattat ctgaacaact
     6061 tgcaagtgaa ggcgacactg tagaagttgg acaagcaatt gctatcatcg gcgaaggtag
     6121 tggcaatgct tctaaagaaa atagtaacga caatactcca caacaaaatg aagaaacaaa
     6181 taataaaaaa gaagaaacaa caaataattc ggtagataaa gctgaagtaa atcaagcaaa
     6241 tgatgacaat cagcaacgta ttaatgctac gccttctgcg cgtcgatatg ctcgtgaaaa
     6301 tggtgtgaat cttgctgaag taagtccgaa aacaaatgat gtggttcgta aagaagatat
     6361 tgataagaaa caacaggcac cggcatcaac acaaacaaca caacaagcat ctgcaaaaga
     6421 agagaaaaaa tacaatcaat atcctacaaa accagtgatt cgtgaaaaaa tgtcacgtag
     6481 aaagaaaaca gctgccaaaa aattattaga ggtatctaat aatacagcta tgttaacaac
     6541 atttaacgaa gttgacatga caaatgttat ggaattgcgt aaacgtaaga aagaacaatt
     6601 tatgaaagat catgatggta ctaaattagg atttatgtca ttctttacta aagcttctgt
     6661 agcagctttg aaaaagtatc cagaagttaa tgcagaaatc gacggcgacg acatgattac
     6721 gaaacaatat tatgatattg gtgtagctgt ttctacagat gatggattat tagtaccatt
     6781 tgtaagagat tgtgataaaa agaattttgc agaaatcgaa gcagaaattg ctaatttagc
     6841 agttaaagca cgagagaaaa aacttggctt agatgatatg gttaatggtt catttacgat
     6901 tacaaatggc ggtatttttg gatcaatgat gagtacgcca attatcaatg gtaatcaagc
     6961 tgcaatctta ggcatgcatt caattattac aagaccaatt gcgattgatc aagatacaat
     7021 cgaaaatcgt ccaatgatgt atattgcatt aagctatgat catagaatta ttgacggtaa
     7081 agaagcagtt ggattcttaa aaacaattaa agaattaatt gaaaacccag aagacttatt
     7141 attagaatct taatccacaa cacaaaatag tttactattc ctaaaagcgg gattaaatca
     7201 ataacaaaca gcagtaagat tatttcctag tcgaaatacc ttactgctgt actttatttt
     7261 tataatgatt cgtattaaaa tgtgggttta attttgacta cttaaaaaat atatcatttc
     7321 tattgaaata gactcacaat agaaatatag gtagaatagc gtgtttcaat atgctaatga
     7381 atgcatttta gataataaca aacgatgaaa tatatgaatt ctataagcgc ctttagttaa
     7441 tttaaaatca tctgaacatg atgtaaattc gctttatgga cacgacatat atatgttttt
     7501 catgtcctac aaaaaacgaa atattcaatt gctatataca gatattcatt aatgacacac
     7561 tcaatagcaa ccataacaat attatgttac ctatttaaat gaaatagttc ttttaaagaa
     7621 atacattttt cacatattaa tctataatca aaatcaactg accgatattc tataatttat
     7681 gattaaaata agttataata taatagtaaa gataaagata gaggtggcta taatgtgtgg
     7741 acttagaagt ataacattag gtacaacaaa tatagaacag acaaaacatt tcatggttga
     7801 catattagga ttaaattatg aagaacttct tgaaaactca attcgtttcg gcgatgcaga
     7861 tataagccca ggaacaagac ttcaatttat acaagttcca agtgagcaat tagaagaatc
     7921 tcactttgtg ggtattggat tacgtacacc aactgactca ggtttagagg agtatgcgga
     7981 aatattatcg aataaggata ttccatttac aacagttaaa gaattaaatg gcaataaata
     8041 tttcagtctc gaagataaca atggtcatat tttctcaata tattcaaacg agaataatta
     8101 tggcgttggt ttaggtatgc cttcttttga gagtgcggtc aatccgttac atcaagtgca
     8161 aggtttagga ccagtgattc ttaaagtgaa tcatgtagat attacaggtc aaattttaac
     8221 aaatatattc ggacttgaag tatttgcaga ataccaaccc ttcgacaatg ctgactatca
     8281 tgtccaagta ttcaaagttg gaacgggtgg tctaggtggc gaaatacatt taatgcctgt
     8341 agaaaccgaa atgacaatgc cggaatatgg cgcagtcgat caagttgagt ttgaaacgaa
     8401 agatgcagat ttctttaatc aagcgaaatc gcgcttagat gaagtggaaa taccatatca
     8461 aacgcttgag caagatgata ttgaatcaat tagaattact gaaaacagtg gattatcgtt
     8521 tatattcact ttacaaaaat aattttttta cgatagcgag gacaaaattt atgttacatg
     8581 aaacttggaa agaacgtaca ccaatcaaga aagtagaagt cattaataca gatgcaaaga
     8641 aatttacagt ttctgacatg ttaacggttg gcaaacaata tgatgttatc aacgaaactg
     8701 aagaatatta tcaaataata gataattctg gattagttgg cggttactat aaaacatatt
     8761 tcaaagaagt ttaaacgcat aattattgaa atgaagccta atatgagatt ttcaattagt
     8821 aaatttggta aaatataaac aattcgcata ctaagcattg cgttttatat cagaatagaa
     8881 tagtaaacta aggcatttgg agaacttatt ccatgtctta gttttttaat ttatttaaag
     8941 gagaacgttt taaaatggca ctaaaacatt ataagaattc agattcaaca gttttcaatg
     9001 atgcgaaggc attatttgat ttaaataaaa atattttact taaaggtcca acaggttcag
     9061 ggaaaacaaa gttggcagaa acattaagtg aagttgttga tacacccatg catcaagtca
     9121 attgttctgt tgatttagat acagaaagct tattaggctt taaaacaatt aaaacaaatg
     9181 cggaaggtca acaagaaatt gtctttgtag atggtccagt tattaaagct atgaaagagg
     9241 ggcatatttt atatattgat gaaataaata tggctaaacc tgaaacattg cctgtattaa
     9301 atggggtctt agattatcgt cgtcaaatta cgaatccata cactggtgaa gtaatcaaag
     9361 ctgtaccagg atttaacgtt atagcagcga taaatgaagg ttatgttggt actttgccaa
     9421 tgaatgaagc actaaaaaat cgctttgttg ttattcacgt tgattatatt gatggggaca
     9481 ttttaaaaaa tgtgattaaa gagcaaagtt tattacaagg tgataaacaa atcgaacaaa
     9541 ttattaagtt taatgaagat ttacgtacta tgtctaagca gggacaaatt tctgaagaag
     9601 ccgctagtat ccgtgcatta ttagacttgt gtgatttaat cactgtaatg ccagttgaac
     9661 gtgcaattaa acgtacaatt attgataaat tggaagatga acgtgaacaa caagcaatat
     9721 ataatgctgt agaactaaac ttttaaagag ggataaaaca tgagtgatcg tttcataaaa
     9781 tttaatgacg aacagttaga tgccaagcaa gtaatgatgt tgcaagactt agctcgactc
     9841 ttattaaaaa atgaacaaac acaagttaaa atccaaaaat tcccttacta taatcctgtt
     9901 caaaatgttt tgattacgag ttggttttgg tcacatcgac caagccatat cgaaatggct
     9961 ggtttaaaaa cagatgttat gttagctgct tacggttatc atatgatgga tgtacaaatt
    10021 gtaaatgaag tagtacaaga taaaactttt aaacacccta aattttatca acaacttttt
    10081 aaattattag aagatatgcg cgtattaaat tctattaaag ttgagcgccc aagtactgct
    10141 aaattaattg atcttcgctt agatacacgt atatcatata cggaatctca aattaaagtg
    10201 tatcgtacta aaacacaata tacagattta ttatttttgt atttagaaca tgcattttta
    10261 agccaagatt tctttgatat accatctata cattcagatt tagatgatat tttagtaaat
    10321 atgttcttat atttacctaa ttttttccaa aatcaaaatt ctgaagataa tatgtatctg
    10381 gctcaaagaa ttatgtatca agtagatgat attttaaaag aagatatgct aaacgaatat
    10441 tattacttac ctaaaacact atataataca ttagctagtc cagagtttga tgatttaaag
    10501 agaactgatg caagtcaagt cgatgggcaa gatgatactt cagaagatga tgataacgaa
    10561 agtgaaaaag ctgactctaa atctgcagat agtgagtcaa aaggtggcgc ctatttagaa
    10621 atggagcttc acgaaggaca aaatagtgaa acattaggta atgatgaagc acgtgaaggc
    10681 gatgctacag atgatatgac tgatatgatg accaaaaagg gcaaaggatc aaacgatacg
    10741 ctaaatcgtg aagaaggcga tgctgttggt caaagccaag catttcaact tgatggtgtg
    10801 aacaaaaatg tagaaataaa atggcaaatt cctgaaattg agccacaata tgttttagaa
    10861 tatcaagaat cgaaacaaga cgtacaatat gaaatcaaag acttaattca aattataaag
    10921 aaaacgattg aacgagaaca acgagatgca cgttttaatt taacaaaagg gcgattacaa
    10981 aaggatttaa ttaattggtt tatcgacgat caatataaat tgttttacaa aaaacaagat
    11041 ttaagtaaat catttgatgc gacatttaca ttgttaattg atgcgtcagc aagtatgcat
    11101 gataaaatgg ctgaaacgaa gaaaggtgta gtgttattcc acgagacgtt aaaagcattg
    11161 aacattaaac atgaaatttt atcattcagt gaggatgcct ttgattcaga cgagcatgca
    11221 caaccaaata tcattaatga aattattaac tatgattact caacctttga aaaagatggc
    11281 ccacgtatta tggcacttga acctcaagat gataatcgtg atggtgttgc tattagagtt
    11341 gcaagtgaac gattaatgcg tcgaaatcaa catcaacgat ttttaatcgt attttcagac
    11401 ggcgaaccgt ctgcatttaa ttatagtcaa gatggtatta ttgatacgta tgaagctgta
    11461 gaaatgtcac gtaaatttgg tattgaagta tttaatgtat ttttgagtca agatccaata
    11521 actgaagatg ttgaacaaac aattcataat atttatggtc aatatgctat tttcgttgaa
    11581 ggtgttgcgc atttacctgg acatctctct ccattattaa aaaaattact acttaaatct
    11641 ttataaataa atttaaaata tgagtttata tgaaattgtg aataaaagtc atcagttact
    11701 atttaaaaag tggatttgaa gaattttatt ttctgacata atttgattta aacaacacat
    11761 gtgttttgtt tctgctatgt acaacatatt tcatatagac tctttattat tttttgtgct
    11821 aaaatattta cttgtaaatt atttcgtgtt ttaaaaaaag gagtaactct atgaataaaa
    11881 atacatgggt cattgggttt acgctcttcg ctatgttttt tggcgcaggc aatctaattt
    11941 ttcctcctaa tctcggattg gatagtggtc aatttttctg gcccgccatt ttagcgtttg
    12001 ttctaactgg gattggttta ccattattag gtgtgattgt aggtgcactt gataaagaag
    12061 gatatattgg cgcattaaat aaaatttcac ctaaattttc aatattgttc ttaatcatca
    12121 tttatttgac tataggacc
//
LOCUS       DANRDI010000077        12102 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.077, whole genome shotgun sequence.
ACCESSION   DANRDI010000077 DANRDI010000000
VERSION     DANRDI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.077"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            40..240
                     /locus_tag="PZ377_001604"
     CDS             40..240
                     /locus_tag="PZ377_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDM6358476.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            268..684
                     /locus_tag="PZ377_001605"
     CDS             268..684
                     /locus_tag="PZ377_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDM6358477.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            916..1215
                     /locus_tag="PZ377_001606"
     CDS             916..1215
                     /locus_tag="PZ377_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDM6358478.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            1345..1689
                     /locus_tag="PZ377_001607"
     CDS             1345..1689
                     /locus_tag="PZ377_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358479.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            1686..3347
                     /locus_tag="PZ377_001608"
     CDS             1686..3347
                     /locus_tag="PZ377_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDM6358480.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            3363..4550
                     /locus_tag="PZ377_001609"
     CDS             3363..4550
                     /locus_tag="PZ377_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDM6358481.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            4534..5271
                     /locus_tag="PZ377_001610"
     CDS             4534..5271
                     /locus_tag="PZ377_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDM6358482.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            5295..6440
                     /locus_tag="PZ377_001611"
     CDS             5295..6440
                     /locus_tag="PZ377_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDM6358483.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            6460..6744
                     /locus_tag="PZ377_001612"
     CDS             6460..6744
                     /locus_tag="PZ377_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358484.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            6734..7018
                     /locus_tag="PZ377_001613"
     CDS             6734..7018
                     /locus_tag="PZ377_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDM6358485.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            7002..7364
                     /locus_tag="PZ377_001614"
     CDS             7002..7364
                     /locus_tag="PZ377_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HDM6358486.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            7361..7765
                     /locus_tag="PZ377_001615"
     CDS             7361..7765
                     /locus_tag="PZ377_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDM6358487.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            7762..8166
                     /locus_tag="PZ377_001616"
     CDS             7762..8166
                     /locus_tag="PZ377_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358488.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            8170..8814
                     /locus_tag="PZ377_001617"
     CDS             8170..8814
                     /locus_tag="PZ377_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDM6358489.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            8841..9080
                     /locus_tag="PZ377_001618"
     CDS             8841..9080
                     /locus_tag="PZ377_001618"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDM6358490.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            9130..9480
                     /locus_tag="PZ377_001619"
     CDS             9130..9480
                     /locus_tag="PZ377_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358491.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            9725..>12102
                     /locus_tag="PZ377_001620"
     CDS             9725..>12102
                     /locus_tag="PZ377_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDM6358492.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFL"
BASE COUNT         4583 a         1606 c         2475 g         3438 t
ORIGIN      
        1 gccgagtgat tacgtctttc gagcggaggt aagtgagtga tgtggattac tatgactatt
       61 gtatttgcta tattgctatt agtttgtatc agtattaata gtgatcgtgc aagggagata
      121 caagcgctca gatatatgaa tgattatcta cttgatgaag tagttaaaac taaaggatac
      181 aacgggttaa aagaatacag gattgaatta aagcgaatga ataacgatat taaaaagtaa
      241 tttatattat cggaggtatt acattgaatg ataaagattg agaaacacga tatcaaaaag
      301 cttgaagaat acattcagca catcgataac tatcgaagag agttgaagat gcgagaatat
      361 gaattacttg aaagtcatga accagataat gcgggagctg gcaaaagtaa tttgccgggt
      421 aacccgattg aacgatgtgc aataaagaag tttagtgata acaggtacaa tacattaaga
      481 aatatagtta acggtgtaga tagattgata gatgaaagtg atgaggatac gcttgagtta
      541 ttaaggttta gatattggga ttgtcctatt ggttgttatg aatgggaaga tatagcacat
      601 tactttggta caagtaagac aagtatatta cgtagaagga atgcactgat cgataagtta
      661 gcaaagtata ttggttatgt gtagcggact tttaccctat gtaagtccgc attaaaacag
      721 tttattatgt tagtatcaga ttaatattta aagttattaa atgctaatac gacgcatgaa
      781 caagaggcgc atcactatgt gatgtgtctt tttatttatg aggtatgaac atgttcaaac
      841 taatagtaaa tacattacta cacatcaagt atagatgcgt cttgatacta cttaagttat
      901 ataaggtgaa acattatgat gactaaagac gaacgcatac gattctataa gtctaaagaa
      961 tggcaaacaa caagaaaaag agtgctagaa agagataatt atgaatgtca acaatgtaag
     1021 agagacggca agttaacgac atatgacaaa agcaagcgta agtcgttgga tgtagatcat
     1081 atattatcgc tagaacatca tccggagttt gctcatgact taaacaattt agaaacactg
     1141 tgtattaaat gtcacaacaa aaaagaaaag agatttataa aaaaagaaaa taaatggaaa
     1201 gacgaaaaat ggtaaatacc cccgggtcaa aaaaatcaaa agcgatcaaa atacttgggg
     1261 aacggggagg ggctcgactt cgcgataatt ttaaaaatcc atgtataccc cccctcttat
     1321 aaccatttta aggcaggtga tgaaatggag attatagttg atgaaaactt agtgcttaaa
     1381 gaaaaagaaa ggctgcaagt attatataaa gacataccta gcaataaatt aaaagtagtt
     1441 gatggtttaa ttattcaagc agcaaggcta cgtgtaatgc ttgattacat gtgggaagac
     1501 ataaaagaaa aaggtgacta tgatttattt actcaatctg aaaaggcgcc accatatgaa
     1561 agggaaagac cagtagccaa actatttaat gctagagatg ctgcatatca aaaaataatc
     1621 aaacaattat cggatttatt gcccgaagag aaagaagaca cagaaacgcc atctgatgat
     1681 tacctatgat tagtaataaa tacgttgatg aatatataaa tttgtggaaa caaggaaaga
     1741 taattttaaa taaagaaaga attgatctct ttaattatct acaaaaacat atatattcac
     1801 gagatgatgt atattttgat gaacagaaaa tcgaggattg tatcaaattt attgaaaaat
     1861 ggtattttcc aacattacca tttcaaaggt ttatcatagc taatatattt cttatagata
     1921 aaaatacaga tgaagctttc tttacagaat ttgctatttt catgggacgt ggaggcggga
     1981 aaaacggtct aataagtgct attagtgatt ttctttctac gcccttacac ggagttaaag
     2041 aatatcacat ctccattgtt gctaatagtg aagatcaagc aaaaacatcg tttgatgaaa
     2101 tcagaaccgt tttaatggat aacaaacgaa ataagacggg taaaacgcca aaagctcctt
     2161 atgaagttag taaagcaaaa ataataaacc gtgcaactaa atcggttatt cgatataaca
     2221 catcaaacac aaaaaccaaa gacggtggac gtgaggggtg tgttattttt gatgaaattc
     2281 attatttctt tggtcctgaa atggtaaacg tcaaacgtgg tggattaggt aaaaagaaaa
     2341 atagaagaac gttttatata agtactgatg gttttgttag agagggttat atcgatgcaa
     2401 tgaagcacaa aattgcaagt gtattaagtg gcaaggttaa aaatagtaga ttgtttgctt
     2461 tttattgtaa gttagacgat ccaaaagaag ttgatgacag acagacgtgg gaaaaggcga
     2521 acccaatgtt acataaaccg ttatcagaat acgctaaaac actgctaagt acgattgaag
     2581 aagaatataa cgatttacca ttcaaccgtt caaataagcc cgaattcatg actaagcgaa
     2641 tgaatttgcc tgaagttgac cttgaaaaag taatagcacc atggaaagaa atactagcga
     2701 ctaatagaga gataccaaat ttagataatc aaatgtgtat tggtggttta gactttgcaa
     2761 acattcgaga ttttgcaagt gtagggctat tattccgaaa aaacgatgat tacatttggt
     2821 taggacattc gtttgtaaga caagggtttt tggatgatgt caaattagaa cctcctatta
     2881 aagaatggga aaaaatggga ttattgacca ttgtcgatga tgatgtcatt gaaattgaat
     2941 atatagttga ttggttttta aaggctagag aaaaatatgg gcttgaaaaa gtcatagctg
     3001 ataattatag aactgatatt gtaagacgtg cgtttgagga tgctggcata aaacttgaag
     3061 tacttagaaa tccaaaagca atacatggat tacttgcacc acgtatcgat acaatgtttg
     3121 cgaaacataa cgtaatatat ggagacaatc ctttgatgcg ttggtttact aataatgttg
     3181 cagtaaaggt taaacccgat ggtaataaag aatatattaa aaaagatgaa aatagaagaa
     3241 aaaccgatgg gttcatggct tttgttcacg cattatatag agcagacgat atagtagaca
     3301 aagacatgtc taaagcgctt gatgcattaa tgagtataga tttctaatag aggaggtgag
     3361 acatgagtat tctagaaaag atatttaaaa ctaggaaaga tataacatat atgcttgatt
     3421 tagatatgat agaagatcta tcacaacaag cgtatgtgaa acgtttagcg attgatagtt
     3481 gtattgaatt tgttgcgcga gctgtcgctc aaagtcattt taaagtattg gaaggtaata
     3541 gaattcaaaa gaatgatgtt tactacaagt taaatataaa accaaatact gacttatcaa
     3601 gcgatagttt ttggcaacaa gttatatata aactaattta tgataacgag gttttaatcg
     3661 tagtaagtga cagcaaagaa ttacttatcg cagatagctt ttacagagaa gagtacgctt
     3721 tgtatgatga tatattcaaa gatgtaacgg ttaaagatta tacttatcaa cgtactttca
     3781 caatgcaaga ggtcatatat ttaaagtaca acaacaataa agtgacacac tttgtagaaa
     3841 gtctattcga agattacggg aaaatattcg gaagaatgat aggtgcacaa ttaaaaaact
     3901 atcaaataag agggattttg aaatctgcct ctagcgcata tgacgaaaag aatatagaaa
     3961 aattacaagc gttcacaaat aaattattca atacttttaa taaaaatcaa ctagcaatcg
     4021 cgcctttgat agaaggtttt gattatgagg aattatctaa tggtggtaag aatagtaaca
     4081 tgcctttttc tgaattgagt gagctaatga gagatgcaat aaaaaatgtt gcgttgatga
     4141 ttggtatacc tccaggtttg atttacggag aaacagctga tttggaaaag aacacgcttg
     4201 tatttgagaa gttctgttta acacctttat taaaaaagat tcagaacgaa ttaaacgcga
     4261 aactcataac acaaagcatg tatttgaaag atacaagaat agaaattgtc ggtgtgaata
     4321 aaaaagaccc acttcaatat gctgaagcaa ttgacaaact tgtaagttct ggttcattta
     4381 caaggaatga ggtgcggatt atgttaggtg aagaaccatc agacaatcct gaattagacg
     4441 aatacctgat tactaaaaac tacgaaaaag ctaacagtgg tgaaaatgat gaaaaagaaa
     4501 aagatgaaaa cactttgaaa ggtggtgatg aagatgaaag cggagattaa aggcgtcatc
     4561 gtttccaacg aagataaatg ggtttacgaa atgcttggta tggattcgac ttgtcctaaa
     4621 gatgttttaa cacaactaga atttagtgat gaagatgttg atattataat taactcaaat
     4681 ggtggtaacc tagtagctgg tagtgaaata tatacacatt taagagctca taaaggcaaa
     4741 gtgaatgttc gcatcacagc aatagcagca agtgcggcat cgcttatcgc aatggctggt
     4801 gaccacatcg aaatgagtcc ggttgctaga atgatgattc acaatccttc aagtattgcg
     4861 caaggagaag cgaaagatct aaatcatgct gcagaaacat tagaacatgt tggtcaaata
     4921 atggctgagg catatgcggt tagagctggt aaaaacaaac aagaacttgt agaaatgatg
     4981 gctagggaaa cgtggctaaa tgctgatgaa gccattgaac aaggttttgc ggatagtaaa
     5041 atgtttgaaa acgacaatat gcaaattgta gcaagcaata cacaagtgtt atcgaaagat
     5101 gtattaaatc gtgtaacagc tttggtaagt aaaacgccag aggttaacat tgatattgac
     5161 gcaatagcaa ataaagtaat tgaaaaaata aatatgaaag aaaaggaatc agaaatcgat
     5221 gttgcagata gtaaagtatc agcaaatgga ttttcaagat tcctttttta atacaaaaaa
     5281 taggaggtca taaaatgact ataaatttat cggaaacatt cgcaaatgcg aaaaacgaat
     5341 ttattaatgc agtaaacaac ggtgaaccgc aagaaagaca aaatgaattg tacggtgaca
     5401 tgattaacca actatttgaa gaaactaaat tacaagcaaa agcagaagct gaaagagttt
     5461 ctagtttacc taaatcagca caatctttga gtgcaaacca aagaagtttc ttcatggata
     5521 tcaataaaaa cgttaactat aaagaagaaa aacttttgcc agaagaaaca attgatagaa
     5581 tttttgaaga tttgacgacg aatcatccgt tattagctga tttaggtatt aaaaacgctg
     5641 gtttgcgttt gaagttctta aaatctgaaa cttctggcgt agccgtttgg ggtaaaatct
     5701 atggtgaaat taaaggtcaa ttagatgctg cgttcagtga agaaacagca attcaaaata
     5761 aattgacagc gtttgttgtt ttaccaaaag atttaaatga ttttggtcct gcgtggattg
     5821 aaagatttgt tcgtgttcaa atcgaagaag catttgcagt ggcgcttgaa actgcgttct
     5881 taaaaggtac tggtaaagac caaccaatcg gcttaaaccg tcaagtacaa aaaggtgtat
     5941 cggtaactga gggtgcttat ccagagaaag aagaacaagg tacgcttaca tttgctaatc
     6001 cgcgcgctac agttaatgaa ttgacgcaag tgtttaaata ccactcaact aacgagaaag
     6061 gtaaatcagt agcggttaaa ggtaatgtaa caatggttgt taatccgtcc gatgcttttg
     6121 aggttcaagc acagtataca catttaaatg caaatggcgt atatgttact gctttaccat
     6181 ttaatttgaa tgttatcgag tctacagtcc aagaagcagg taaggtttta acgtacgtta
     6241 aaggtttata tgatggttat ttagctggtg gtattaatgt tcagaaattt aaagaaacac
     6301 ttgcgttaga tgatatggat ttatacactg caaaacaatt tgcttacggc aaagcgaaag
     6361 ataataaagt tgctgctgtt tggaaattag atttaaaagg acataagcca gctttagaag
     6421 gtaccgaaga aacactataa aattttatga ggtgataaaa tggtgaaatt taaagttgtt
     6481 agagctttta aagacataga gcacaatcaa cacaagtaca aagtagggga gttgtatcca
     6541 gctgaagggt ataacaatcc tcgtgttgaa ttgttgacaa atcaaatcaa aaataagtac
     6601 gacaaagttt atatcgtacc tttagataag ctgacaaaac aagaattatt agaactatgc
     6661 gaatcattac aaaaaaaagc gtctagttca atggttaaaa gtgaaatcgt cgacttattg
     6721 aatggtgaag acaatgacga ttgatgattt gcttgtcaaa tttaaatcac ttgaaaagat
     6781 tgaccataat tcagaggatg agtacttaaa gcagttgtta aaaatgtcgt acgagcgtat
     6841 aaaaaatcag tgcggagttt ttgaattaga gaatttaata ggtcaagaat tgatacttat
     6901 acgcgctaga tatgcttatc aagatttatt agaacacttc aacgataatt acagacctga
     6961 aataatagat ttttcgttat ctctaatgga ggtatcagaa gatgaagaaa gtgtttaaaa
     7021 aacctagaat tacaactaaa cgtttaaata ctcgtgttca tttttataag tatactgaaa
     7081 ataatggtcc agaagctgga gaaaaagaag aaaaattatt atatagctgt tgggcgagta
     7141 ttgatggtgt ctggttacgt gaattagaac aagctatctc aaacggaacc caaaatgaca
     7201 ttaaattgta tattcgtgat ccgcaaggtg attatttacc cagtgaagaa cattatcttg
     7261 aaattgaatc aagatatttc aaaaatcgtt tgaatataaa gcaagtatca ccagatttgg
     7321 ataataaaga ctttattatg attcgtggag gatatagttc atgagtgtga aagtgatagg
     7381 tgataaagca ttagaaagag aattagaaaa tcgtttcgga actcaagcga tgttaaaagt
     7441 tcaagatagg gcgctaattg ctggtgctaa agtaattgtt gaagaagtaa aaaaacaact
     7501 aaagccatca aaagatacgg gcgcattaat taatgaggta agttttagta aacctgaatg
     7561 gataaacgga aaacgtacaa ttactgttca ttggcgaggt tctaaagacc gttataaaat
     7621 cgtacattta attgaaaatg gacacgttca aaaagggaca ggtaaattta tcaaacctaa
     7681 agctatgggt ggcgttaata gagcaataag acaagggcaa aataagtatt ttgagacgct
     7741 aaaaagggag ttaaaaaaat tgtgattgat attttgtaca aagttcatga agtgattagt
     7801 caagacagaa ttattagaga gcacgtaaat atcaataata ttaagttcaa taaataccca
     7861 aacgtaaaag atacggatgt acctttcata gttattgacg atatagacga cccaatacct
     7921 acaacttata ctgacggaga tgagcgcgca tatagttaca ttgtacaaat agatgtattt
     7981 gtgaagttta acgaaaagta taacgcgaga atcataagaa ataagatatc taatcgtatt
     8041 caaaagttat tatggtctga actgaaaatg ggaaatgtat ccaacggaaa acctgaatat
     8101 atagaagaat ttaaaacata tagaagcact cgtgtctacg agggcatttt ttacgaggag
     8161 gaataataaa tggtagtaaa acaagcaagt gcgccgaaag cgtattttaa cattactggt
     8221 ttaggtttcg ctaaattaac aaaagaaggc gcaaagctag aatatagcga tattacaaaa
     8281 actagaggat tacaaaaaat cggtgttgaa actggaggag acttgaaaac agcatatgct
     8341 gatggtggtc caatcgaatc agggaacaca gacggagaag gtaaaatttc gttacaaatg
     8401 catgctttcc ctaaagagat tcgcaaaata gtattcaatg aagagtatga tgaatacggt
     8461 gtttataaag aaactcaagg taaacaaaac aactatgtag caatttggtt cagacaagag
     8521 cgtcgagacg gcacatttag aacggtttta ttacctaaag tcatgtttac aaatcctaaa
     8581 atcgatggcg aaacggctga gaaagattgg gatttctcaa gcgaagaggt tgaaggtgag
     8641 gcacttttcc ctttaattga taataaaaag tcagtacgta aatatatctt tgattcagca
     8701 aacatgacaa atcatagtgg aaatggtgaa aaaggcgaag aggctttctt aaagaaaatt
     8761 ttaggcgaag aatatactgg aaacgtgaca gaggataacg gcgaaacttt gtaacgcaac
     8821 cggcttcatc ggaaactgag ataaagtcgg ttaatatacc agatagtttt aaaacactaa
     8881 aagttggcaa cacatatgat ttaaatgttg tagtagagcc atctaatcaa agtaagttat
     8941 tgaaatacac aacagatcaa acgaatattg tatcaatcaa tagtgatggt caagtcactg
     9001 cggaagcaca aggtattgcc acggttaaag catatgttgg taatatgagt gacactataa
     9061 caataaatgt agaagcataa gaggggacaa cccctctatt ttatttgaaa ataaggagag
     9121 tattataaaa tggcaaaatt aaaacgtaac attattcaat tagtagaaga tccaaaagca
     9181 aatgaaatta aattacaaac gtacttaaca ccacacttca tttcatttga aattgtatac
     9241 gaagcaatgg atttaatcga tgatattgag gacgaaaata gcacgatgaa gccaagagaa
     9301 atcgctgaca gattgatgga tatggttgta aaaatttacg ataaccaatt cgcagttaaa
     9361 gacctaaaag aacgtatgca tgcacctgat ggaatgaatg cacttcgtga acaagtggtt
     9421 ttcattacgc aaggtcaaca aactgaggaa actagaaatt ttatccagaa catgaaataa
     9481 agcctgaaga tttaacatat aaagcaatgt tgaaaaatat ggatactctc atgatggact
     9541 taattgaaaa tggtaaagac gctaacgaag ttttaaaaat gccatttcat tatgtgcttt
     9601 ccatatatca aaataaaaac aatgacattt ctgaagaaaa agcagaggct ttaattgatg
     9661 cattttaacc ttaaccgttt ggttagggtt atttttttga acttttttag aaaggaggta
     9721 aaaaatggga gaaagaataa aaggtttatc tataggtttg gatttagatg cagcaaattt
     9781 aaatagatca tttgcagaaa tcaaacgaaa ctttaaaact ttaaattctg acttaaaatt
     9841 aacaggcaac aacttcaaat ataccgaaaa atcaactgat agttacaaac aaaggattaa
     9901 agaacttgat ggaactatca caggttataa gaaaaacgtt gatgatttag ctaagcaata
     9961 tgacaaggta tctcaagaac agggcgaaaa cagtgcagga gctcaaaagt tacgacaaga
    10021 atataacaaa caagcaaatg aactgaatta tttagaaaga gaattacaaa aaacatcagc
    10081 cgaatttgaa gagttcaaaa aagctcaagt tgaagctcaa agaatggcag aaagtggctg
    10141 gggaaaaacc agtaaagttt ttgaaagtat gggacctaaa ttaacaaaaa tgggtgatgg
    10201 tttaaaatcc attggtaaag gcatgatgat tggtgtaact gcacctgttt taggtatcgc
    10261 agcagcatca ggaaaagctt ttgcagaagt tgataaaggt ttagatactg ttactcaagc
    10321 aacaggcgca acaggcagtg aattaaaaaa attgcagaac tcatttaaag atgtttatgg
    10381 caattttcca gcagatgctg aaactgttgg tggagtttta ggagaagtta atacaagatt
    10441 aggttttaca ggtaaagaac ttgaaaatgc cacagagtca ttcttgaaat tcagtcatat
    10501 aacaggttct gacggtgtgc aagccgtaca gttaattacc cgtgcaatgg gcgatgcagg
    10561 tatcgaagca agtgaatatc aaagtgtttt ggatatggta gcaaaagcgg cgcaagctag
    10621 tgggataagt gttgacacat tagctgatag tattactaaa tacggcgctc caatgagagc
    10681 tatgggcttt gagatgaaag aatcaattgc tttattctct caatgggaaa agtcaggcgt
    10741 taatactgaa atagcattca gtggtttgaa aaaagctata tcaaattggg gtaaagctgg
    10801 taaaaaccca agagaagaat ttaagaagac attagcagaa attgaaaaga cgccggatat
    10861 agctagcgca actagtttag cgattgaagc atttggtgca aaggcaggtc ctgatttagc
    10921 agacgctatt aaaggtggtc gctttagtta tcaagaattt ttaaaaacta ttgaagattc
    10981 ccaaggcaca gtaaaccaaa catttaaaga ttctgaaagt ggctccgaaa gatttaaagt
    11041 agcaatgaat aaattaaaat tagtaggtgc tgatgtatgg gcttctattg aaagtgcgtt
    11101 tgctccagtc atggaagaat taataaaaaa gctatcagtc gctgtcgatt ggttttcgaa
    11161 tttaagtgat ggttctaaaa gatcaattgt tattttcggt ggtattgctg ctgcaattgg
    11221 tcctgtagtt tttgggttag gtgcatttat aagcacaatt ggtaatgcag taactgtatt
    11281 agccccacta ttagctggta ttgcaaaggc tgatggatta attagttttt tatcgactaa
    11341 agtacctata ttaggaactg tcttcacagc tttaactggt ccaattggca ttgtattagg
    11401 tgttttagct ggcctagcag tcgcatttac aattgcttat aagaaatctg aaacatttag
    11461 aaattttgtt aatgatgcaa ttgaaagtgt taaacaaaca tttagtaatt ttattcaatt
    11521 tattcaacct ttcattgatt ccgttaaaaa cgtctttaaa caagcggttt cagcaatcgt
    11581 tgatttcgct aaagatattt ggagtcaaat taatggattc tttaatgaaa acggaatttc
    11641 tattgttcaa gcgcttcaaa atatatgcaa ttttatcaaa gttatatttg aatttatctt
    11701 aaattttgta attaaaccaa taatgtttgc aatctggcaa gtgatgcaat ttatttggcc
    11761 ggcggttaaa gccttgattg tcagtacttg ggagaatata aaaggtgtga tacaaggaac
    11821 tttaaatatc atactaggtt taattaagtt cttctcaagt ttatttactg gagattggcg
    11881 aggagtttgg gacgcgatta ttatgattct taaaggagtc gttcaattaa tatggaattt
    11941 aattcaatta tggtttgtag gcaaaatact tggcgttgtt aggtactttg gcggattgct
    12001 aaaaggatta ataacaggta tttgggacgt aataaaaagt atattcagta aatctttatc
    12061 agcaatttgg aatgcgacaa aaagtatttt tggattctta tt
//
LOCUS       DANRDI010000078        12065 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.078, whole genome shotgun sequence.
ACCESSION   DANRDI010000078 DANRDI010000000
VERSION     DANRDI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.078"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(49..753)
                     /locus_tag="PZ377_001621"
     CDS             complement(49..753)
                     /locus_tag="PZ377_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDM6358493.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            998..1192
                     /locus_tag="PZ377_001622"
     CDS             998..1192
                     /locus_tag="PZ377_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDM6358494.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            1204..1455
                     /locus_tag="PZ377_001623"
     CDS             1204..1455
                     /locus_tag="PZ377_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDM6358495.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            1493..2617
                     /locus_tag="PZ377_001624"
     CDS             1493..2617
                     /locus_tag="PZ377_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDM6358496.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            2633..3070
                     /locus_tag="PZ377_001625"
     CDS             2633..3070
                     /locus_tag="PZ377_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDM6358497.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            3494..4450
                     /locus_tag="PZ377_001626"
     CDS             3494..4450
                     /locus_tag="PZ377_001626"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDM6358498.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            4650..5129
                     /locus_tag="PZ377_001627"
     CDS             4650..5129
                     /locus_tag="PZ377_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDM6358499.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            5144..5983
                     /gene="fakB2"
                     /locus_tag="PZ377_001628"
     CDS             5144..5983
                     /gene="fakB2"
                     /locus_tag="PZ377_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDM6358500.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            6069..6602
                     /gene="msrA"
                     /locus_tag="PZ377_001629"
     CDS             6069..6602
                     /gene="msrA"
                     /locus_tag="PZ377_001629"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDM6358501.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            6595..7023
                     /gene="msrB"
                     /locus_tag="PZ377_001630"
     CDS             6595..7023
                     /gene="msrB"
                     /locus_tag="PZ377_001630"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDM6358502.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            7035..7535
                     /locus_tag="PZ377_001631"
     CDS             7035..7535
                     /locus_tag="PZ377_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDM6358503.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            7535..7756
                     /locus_tag="PZ377_001632"
     CDS             7535..7756
                     /locus_tag="PZ377_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDM6358504.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            7948..9438
                     /locus_tag="PZ377_001633"
     CDS             7948..9438
                     /locus_tag="PZ377_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDM6358505.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            9838..10347
                     /locus_tag="PZ377_001634"
     CDS             9838..10347
                     /locus_tag="PZ377_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6358506.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            10359..11429
                     /locus_tag="PZ377_001635"
     CDS             10359..11429
                     /locus_tag="PZ377_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDM6358507.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            11446..12060
                     /locus_tag="PZ377_001636"
     CDS             11446..12060
                     /locus_tag="PZ377_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDM6358508.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
BASE COUNT         4490 a         1704 c         2140 g         3731 t
ORIGIN      
        1 atttctgtcc cactccctta aaacttattc ttttgtgtag taagtgcgtt aatagccttg
       61 atctaactta tcaatcttac ctttacgata aaatgattta gcaatatatc caaatggtac
      121 attgaaaact gttgaagcta attttaatac gtacgttgta ataaatattt caaatacaac
      181 tgtaccaggt aaacttccga taaatgcgat agctacaaat aaagctgtat caattattga
      241 gcttaaaaat gtactaccat atgcacggat gaaaaacgtt ttatcagaac taaatacttt
      301 tttaattagt gaaaagataa atacatcaat atgttgacca ataatatatg cgacgattga
      361 gcctaaagca atgcgtggca caacatcaaa gattgcgtgt aatgcttttt gtgccatatc
      421 ttctggtgca ggaataaaat gtaatgacaa ttgcataaca ataatcataa ttaatgttga
      481 tgaaaagcct aaccaaactg ctctttttgc aactctacgc ccataaatat cgtttaatat
      541 atctgttgct aaataaatag aagcaaacat gacattacct aaagttgctg aaataccaaa
      601 gatttctaca gttttaatca cttgtatgtt ggcaatgatt gtgccaattg caacccatgc
      661 aattaaacct tgtttaccaa aaaagcgata cataagtacc ataagcacga acgttgcaat
      721 aaacgtaact agtcctaaaa tttcattata catattaaaa tgtcctccta aattttgatc
      781 atgcgggtgt ttagaaaccg ctcaataaat aactaactca ttttactgaa aaatcgtttg
      841 cgatgcaata gcatttgaat ataaatacat tttacgtata gaattgtaaa aggtttcatt
      901 caaatcttag ggtcaaaaat gttataatat ttttatgtca aatttaaaac agtaacactt
      961 atttacaagg ttgcaatatt ttgaagtaat aaaggaagtg tcgcgtattt taactttttc
     1021 agagcaaaat gcactcgcga aaatagatga tttaatgaat acttattgca atcaatgtcc
     1081 aatcaaaact cgtctgcgta aattagaggg gaaaacgaag gcgcatcatt tttgtatcaa
     1141 tgagtgttca atagggaaag aaataaaaca attaggaaat gaacttcaat aggaggaagt
     1201 caaatgaaaa ttatatctat atcagaaaca ccgaaccaca acacaatgaa gattacactt
     1261 agtgaaagca gagaaggtat gacatcagat acgtatacta aagttgatga ttcacagcca
     1321 gcatttatta atgacatctt aaaggttgaa ggcgttaaat caattttcca tgttatggac
     1381 tttatttcag tagataaaga aaatgacgca aattgggaaa cagtattgcc aaaagtagag
     1441 gctgtattcg aataaatttt tcatcaacta gtattcgggg ggaataaagt atatggaaat
     1501 tttacgtata gagccaacac caagtccaaa tacaatgaaa gttgttttgt catatacaag
     1561 agaagacaag ttatctaata cttataaaaa agtagaagaa acacaaccaa gatttataaa
     1621 tcagttgtta tctatagatg gtatcacttc catttttcat gtcatgaact tcttagctgt
     1681 tgataaggca ccaaaagctg attgggaagt catattacct gatattaaag ctgctttttc
     1741 tgatgcgaat aaggttttag aatctgtaaa tgaacctcaa attgacaatc attttggtga
     1801 aattaaagct gaattattaa cttttaaggg tataccgtat caaattaagc taacttctgc
     1861 tgaccaagaa ttaagagaac aattaccaca aacatatgtt gaccatatga ctcaagcgca
     1921 aacagcacat gacaatattg tttttatgcg taaatggcta gatttaggaa atcgctatgg
     1981 aaatattcaa gaagtaatgg atggtgtcct agaagaagtg ctaactacct atccagaatc
     2041 acagttaccc gtattggtaa aacatgcttt agaagaaaat cacgcaacta ataattatca
     2101 tttctatcga catgtctctt tggatgaata tcatgcaact gataattgga agactcgatt
     2161 acgaatgtta aaccattttc caaagccgac ttttgaagat ataccgctgc ttgatttagc
     2221 tttatctgat gaaaaagtac cggttagacg tcaagcgatt gtattattag gtatgattga
     2281 aagtaaagaa attttaccgt atttatataa ggggcttcgt gataaaagtc ctgttgtaag
     2341 aagaacagca ggggattgca taagcgattt agggtatcca gaggcactac cagaaatggt
     2401 gctactatta gatgatccac agaaaatcgt taggtggcgt gctgctatgt ttatctttga
     2461 tgaaggtaat gcagagcagc ttcccgcact aaaagcccat attaatgaca atgcgtttga
     2521 agttaaatta caaattgaaa tggccatatc tcgtattgaa aacggagatg aagcacttgg
     2581 ttcagtatgg aaacaaatgg caaacagaac aatttaattg gaggaattaa atatgaatgc
     2641 atatgatgct tatatgaaag aaattgcgca acaaatgcgt ggcgaattaa ctcaaaatgg
     2701 ttttacaagt ttagaaacga gcgaagcagt atcggagtat atgaaccaag taaatgctga
     2761 tgacactact tttgtagtta ttaactctac atgcggctgt gcagctggat tagcaagacc
     2821 agctgcagta gcagttgcaa cacaaaatga acatagacct acaaatacag ttacagtttt
     2881 tgctgggcaa gataaagaag caactgctac aatgcgagaa ttcattcagc aagcaccatc
     2941 tagtccttcg tatgctttat tcaaaggtca agatttagtt tattttatgc ctagagaatt
     3001 tatcgaaggt agagatatta atgacattgc aatggactta aaggatgcct ttgacgaaaa
     3061 ttgtaaatag tacacataaa taaatataaa ggttaacaca ttttataata ttaaaaatgg
     3121 tgtctgtcat tgaaaataga gaatatagtt gtattctatt tgttaaataa agtccgtttt
     3181 taccaactat attttctaga aatttaactg ttttaatagg acatcaaaca taatattcaa
     3241 atcatgtgtt aacctctttt ttaaaatttt ttagcattaa agttatagat ttgggtaaac
     3301 aattaccaat tggaaacata tatcacgtta cgatggggta ggtacttaat cagcatttta
     3361 taaataaagt aacggaattc atgatattaa tatcatattc ctaaaatgag tgataacaaa
     3421 atgctacata aagttaagtt atatcaaact aaatatacat actataaata atgaaaatga
     3481 ggtgttatcg catatgttga attcatttga tgcagcatat cacagtcttt gtgaagaagt
     3541 tttagaaata ggaaatacac gaaatgatcg cacaaataca ggtacgattt cgaaatttgg
     3601 tcatcaactt cgctttgact tatctaaagg atttccacta ttaacgacaa agaaagtttc
     3661 ttttaaatta gtagcaaccg aattattatg gttcattaaa ggagatacaa acatccaata
     3721 cttattaaaa tataataata atatatggaa cgaatgggct tttgaaaatt atatcaaatc
     3781 agacgagtat aaaggtccag atatgacaga tttcgggcat cgtgcattga gtgatcctga
     3841 atttaacgaa caatataaag aacaaatgaa acaatttaag caacgtattc ttgaagatga
     3901 tacatttgcg aagcaattcg gggatttagg aaatgtttat ggtaaacaat ggcgagattg
     3961 ggttgataaa gatggtaatc attttgatca acttaaaaca gtaattgaac aaattaagca
     4021 taatccagat tcaaggcgac acatcgtatc tgcatggaat ccaacagaaa ttgatacaat
     4081 ggcacttccg ccttgtcata ccatgttcca gttttatgtc caagatggta agttaagttg
     4141 ccagttatac caacgtagcg cagatatctt tttaggtgtg ccatttaata tcgcaagcta
     4201 cgctttattg acacacctta ttgccaaaga atgtggactt gaagtgggtg aatttgtgca
     4261 tacatttgga gatgcacata tttattcaaa tcatattgat gcgattcaaa cacaattagc
     4321 acgtgaaagc ttcaatcctc caacattaaa aattaacagt gacaagtcta ttttcgacat
     4381 aaattatgaa gatttggaaa ttgttgacta tgaatcacat ccagcaataa aagctccaat
     4441 agcagtgtag tcattgcata gttagctaac catatagaca tcaaaatgac atcatagtat
     4501 tttcaagtgc aaaaaagtac ttttttgtgt taaacgtttt cataaattat gcaaaatcat
     4561 tatttctatc acactttatg ataaaaattg tgttaaatta aagataactt agtaataaaa
     4621 aatgaaatga tagaagaagg aggataatta tgactttatc cattctagtt gcacatgact
     4681 tgcaacgagt aattggtttt gaaaatcaat taccttggca cctaccaaat gatttgaagc
     4741 atgttaaaaa attatcaaca ggtcatactt tagtaatggg tcgtaagaca tttgaatcga
     4801 ttggtaaacc actaccgaat cgtcgaaatg ttgtacttac ttcagataca agtttcaacg
     4861 tagagggcgt tgatgtaatt cactctattg aagatattta ccaactaccg ggccatgttt
     4921 tcatatttgg agggcaaaca ttatttgaag aaatgattga taaagtggac gacatgtata
     4981 ttactgttat tgaaggtaaa ttccgtggtg atacgttctt tccaccttat acatttgaag
     5041 actgggaagt tgcctcttca gttgaaggta aactagatga gaaaaataca attccacata
     5101 cctttctaca tttaattcgt aaaaaataag ggggaaaacg accatgacaa aacagattat
     5161 agtaacagac tcaacatccg atttatctaa agaatactta gaagcaaaca acattcatgt
     5221 aattccttta agtttaacta ttgaaggagc ttcatacgtt gaccaagtag atattacatc
     5281 agaagaattt attaatcata ttgaaaatga tgaagatgta aagacaagtc agccagccat
     5341 aggtgaattt atatctgctt atgaagaact aggaaaagat ggctctgaaa tcataagtat
     5401 tcatctttct tcaggattaa gtggtacata taacactgct taccaagcaa gtcaaatggt
     5461 agatgctaat gtaactgtta ttgattcaaa atctatttct tttggtttag ggtatcaaat
     5521 acaacaccta gtagagcttg taaaagaagg tgtctcaact tctgaaatag ttaaaaagtt
     5581 aaatcattta agagaaaaca ttaaattatt tgtagttata gggcaattga atcaattaat
     5641 taaaggtggc agaattagta aaacaaaagg tttgattggt aatcttatga aaattaaacc
     5701 aattggtaca ctagatgatg gtcgcttaga gcttgtgcac aatgcgagaa ctcaaaattc
     5761 tagtatccaa tacttgaaaa aggaaattgc tgaatttata ggagatcatg aaatcaaatc
     5821 cattggtgtc gcacatgcta acgtcattga atatgttgat aaattgaaga aagtttttaa
     5881 tgaagctttt catgtgaata attacgatat aaatgtaact acaccagtta tttctgcaca
     5941 tactggtcaa ggtgcgattg gcctcgtagt ccttaagaag taaatttaat cttttcagtg
     6001 ttaattactt ccatttcaat cctttataga ctaaatttat aattagatag atagaggagg
     6061 taattcatat gacaaaagaa tatgcaacat tagcaggagg atgtttctgg tgcatggtta
     6121 aaccatttac atcatatcca ggcatcaagt cagtcgtatc tggttatagt ggcggtcatg
     6181 ttgacaaccc aacttatgaa caggtatgta cgaatcaaac cggccatgtc gaagcagtac
     6241 aaattacgtt tgatccagag gttacttcct ttgaaaatat attagacata tatttcaaaa
     6301 catttgaccc aactgatgat caagggcaat ttttcgatag aggcgaaagc tatcaaccag
     6361 tcattttcta tcatgatgaa catcagaaaa aggctgctga gtttaaaaag caacaattaa
     6421 atgaacaagg tattttcaag aaaccagtga ttacacctat taaaccatat aaaaatttct
     6481 atccagctga agactaccat caagattatt acaaaaagaa cccggtacat tattaccaat
     6541 atcaacgtgg ttcaggtaga aaagcgttta tagaatcaca ttgggggaat caaaatgctt
     6601 aaaaaagata aaagtgaact aacagatata gaatatattg ttacacaaga aaacggcact
     6661 gaaccaccat ttatgaatga atattggaat cattttgcta aaggaattta tgtagataaa
     6721 atttctggta aacctttatt tacatctgaa gaaaagtttc attctgaatg tggatggcct
     6781 agcttttcca aagcgcttga tgacgatgaa attatagaat tagtcgacaa atcatttggt
     6841 atgttgagaa ctgaagtgcg ttcagaagaa tcaaatagtc atttaggaca tgtctttaat
     6901 gatggaccta aagaaagtgg cggcttaaga tactgtatca attccgctgc aattcaattt
     6961 attccatatg aaaagttaga agaattgggt tatggcgatt taatatcaca ttttgataag
     7021 taggagtgga aagtatgttt aaaaaattat tcggaaaagg caaagaagtt cagaaagata
     7081 tcgcaattta tgcaccatta actggagaat tcgtgaaaat tgaagatatt ccagatcctg
     7141 tattcgcaca aaaaatgatg ggcgaaggtt ttggtattaa tccaactgaa ggagaagttg
     7201 tgtctccaat tgcaggacgt gttgacaatg tctttccaac taagcatgcg attgggttaa
     7261 aagcagataa cggattagaa ttattagttc atatcggttt agacacagtt caattagacg
     7321 gtgaaggatt tgaagtgtta gtgtctagtg gtgacgaagt taacgtcgga gatccattag
     7381 taagattcaa ccttgaatat attaataata acgctaaatc tgtaatttca ccaattataa
     7441 ttactaacac tgatcaagca gcttcaatta atatttatga tgaaaatgct gtgattaaag
     7501 gtgaaacaaa agtgattgat gtgacaatga actaatgaaa aactattcgt tttatcaatt
     7561 tgtcatgaca gttcgtggtc gacacgacga taaaggtcgt ctagcagaag agatatttga
     7621 cgatcttgct ttcccaaaac acgatgatga ttttaacata ctgtctgatt atattgagac
     7681 acatggtgat ttcacattgc caatgtctgt atttgatgat ttatatgaag aatatacgga
     7741 atggctaaaa ttttaatata atttttaata ataatagtta gaaccagggt gatgcaattc
     7801 gttatcctgg ttttaattta aaataaacta agttgtgact aaaaattaat caattatagt
     7861 gaaatatggt gcgctatctt gcataaattg atatgattaa ctacacagaa tttaaaagta
     7921 cataatacat aataaggaag tgatacaatg gatgataagc aacacacatc ttcatccgat
     7981 gatgaacgcg ctgaaattgc aacaagcaat caagaccaag aaactaattc atcgaaacgc
     8041 gttcacttaa aacgttggca attcatatca atattaattg gtacaatcct aataacagct
     8101 gtcatcacag ttgttgcata tatttttata aatcaaaaaa taagtggttt aaacaaaact
     8161 gatcaagcaa acttaaataa aattgaaaat gtgtataaaa tcttaaatag tgattattac
     8221 aaaaaacagg actctgacaa gttaagtaaa gctgcaattg atggcatggt caaagaatta
     8281 aaagatcctt attctgaata tttaacaaaa gaacaaacga aatcctttaa tgaaggtgtt
     8341 tcaggtgatt ttgtaggtat tggtgcagaa atgcaaaaga aaaatgatca aattatggtt
     8401 actagtccta tgaagggatc tccagcagaa cgtgctggca ttcgtcctaa agatgtcatt
     8461 actaaagtaa atggaaaatc aattaaaggt aaagcattag atgaagttgt caaagatgtt
     8521 cgtggtaaag aaaacactga agtcacttta actgttcaac gaggtagtga agaaaaagac
     8581 gttaagatta aacgtgaaaa aattcatgtt aaaagtgttg agtataagaa aaaaggtaaa
     8641 gttggagtta ttactattaa taaattccag aatgatacat caggtgaatt gaaagatgca
     8701 gttctaaaag ctcacaaaga tggtttgaaa aagattgttt tagatttaag aaataatcca
     8761 ggtggactac tagatgaagc tgttaaaatg gcaaatattt ttatcgataa aggaaaaact
     8821 gttgttaaac tagaaaaagg taaagatact gaagcaattc aaacttctaa tgatgcgtta
     8881 aaagaagcga aagacatgga tatatccatc ttagtgaatg aaggttcagc tagcgcttct
     8941 gaagtgttta ctggtgcgct aaaagactat aataaagcta aagtttatgg gtcaaaaaca
     9001 ttcggcaaag gtgtcgtaca aactacaaga gagtttaagg atggttcatt gttaaaatat
     9061 actgaaatga aatggttaac gccagatggt cattatattc acggtaaagg cattaaacca
     9121 gacgttacta ttgacacacc taaatatcaa tctttaaatg tcattcctaa tacgaaaaca
     9181 tttaaagttg gagacgatga taaaaatatt aaaactatta aaattggttt atcagcttta
     9241 ggttataaag ttgataatga atcaacgcaa tttgataaag ctttagaaaa tcaagttaaa
     9301 gctttccaac aagcgaataa acttgaggta actggtgaat ttaataaaga aacgaataat
     9361 aaatttactg agttattagt tgaaaaagct aataaacatg atgatgttct cgataagttg
     9421 attaatattt taaaataagc gatacacact actaaaattg tattattatt atgttaatga
     9481 cacgcctcct aaatttgcaa agatagcaat ttaggaggcg tgtttatttt tattgacgtc
     9541 taactctaaa agatataaat tagacattta caaatgatgt aaataacgca atttctatca
     9601 tcgctgataa caattcatgg tttaatatgc aatgagcata tactttttaa atagtattat
     9661 tcactagttt taacaatcaa ttaattggta tatgatactt ttattggtta tttttatccc
     9721 atagtgtgat aattactatt tttcattcat aataaaggtt taaagcatgt taatagtgtg
     9781 taagattaac atgtactgaa aaacatgttt aacataatga tataaggagt gacgtacatg
     9841 atccgtctag gtaaaatgtc agatttagat caaatcttaa atctagtaga agaagcaaaa
     9901 gaattaatga aagaacacga caacgagcaa tgggacgatc agtacccact tttagaacat
     9961 tttgaagaag atattgctaa agattatttg tacgtattag aggaaaatga caaaatttat
    10021 ggctttattg ttgtcgacca agaccaagca gaatggtatg atgacattga ctggccagta
    10081 aatagagaag gcgcctttgt tattcatcga ttaactggtt cgaaagaata taaaggagct
    10141 gctacagaat tattcaatta tgttattgat gtagttaaag cacgtggtgc agaagttatt
    10201 ttaacggaca cctttgcgtt aaacaaacct gcacaaggtt tatttgccaa atttggattt
    10261 cataaggtcg gtgaacaatt aatggaatat ccgccatatg ataaaggtga accattttat
    10321 gcatattata aaaatttaaa agaatagagg taatattaat gacgaaaatc gcatttaccg
    10381 gagggggaac agttggacac gtatcagtaa atttaagttt aattccaact gcattatcac
    10441 aaggttatga agcgctttat attggttcta aaaatggtat tgaaagagaa atgattgaat
    10501 cacaactacc agaaattaag tattatccta tttcgagtgg taaattaaga agatatattt
    10561 ctttagaaaa tgccaaagac gtatttaaag tattgaaagg tattcttgat gctcgtaaag
    10621 ttttgaaaaa agaaaaacct gatctattat tttcaaaagg tggatttgta tctgtgcctg
    10681 ttgttattgc agccaaatca ttaaatatac caactcttat tcatgaatct gacttaacac
    10741 caggattagc gaataagata gcacttaaat ttgccaagaa aatatataca acatttgaag
    10801 aaacgctaaa ctacttacct aaagagaaag ctgattttat tggagcaaca attcgagaag
    10861 atttaaaaaa tggtaatgca cataatggtt atcaattaac aggctttaat gaaaataaaa
    10921 aagttttact cgttatgggt ggaagcttag gaagtaaaaa attaaatagc attattcgcg
    10981 aaaacttaga tgcattatta caacaatatc aagtgataca tttaactggt aaaggattaa
    11041 aagatgctca agttaaaaaa tcaggatata tacaatatga atttgttaaa gaggatttaa
    11101 cagatttatt agcaattacg gatacagtaa taagtagagc tggatcaaat gcgatttatg
    11161 agttcttaac attacgtata ccaatgttat tagtaccatt aggtttagat caatcccgag
    11221 gcgaccaaat tgacaatgca aatcattttg ctgataaagg atatgctaaa gcgattgatg
    11281 aagaacaatt aacagcacaa attttattac aagaactaaa tgaaatggaa caggaaagaa
    11341 ctcgaattat caataatatg aaatcgtatg aacaaagtta tacgaaagaa gctttatttg
    11401 ataagatgat taaagacgca ttgaattaat ggggggtaat gctttatgag tcaatggaaa
    11461 cgtatctctt tgctcatcgt ttttacattg gtttttggaa ttatcgcgtt tttccacgaa
    11521 tcaagacttg ggaaatggat tgataatgaa gtttatgagt ttgtatattc atcagagagc
    11581 tttattacga catctatcat gcttggggct actaaagtag gtgaagtctg ggcaatgtta
    11641 tgtatttcat tacttcttgt ggcatatctc atgttaaagc gccacaaaat tgaagcatta
    11701 ttttttgcat taacaatggc attatctgga attttgaatc cagcattaaa aaatatattc
    11761 gatagagaaa gacctacatt gctgcgttta attgatataa caggatttag ttttcctagc
    11821 ggtcatgcta tgggatcaac tgcatatttt ggaagtggta tctatctatt aaatcgatta
    11881 aatcaaggta attcaaaagg tattcttata gggttatgtg cagctatgat tttattgatt
    11941 tccatatcac gtgtatatct aggtgtacat tatccaacag atattattgc cggcattatt
    12001 ggtggattat tttgcattat tttatcaacg ttattactta gaaataaatt aataaattaa
    12061 atagt
//
LOCUS       DANRDI010000079        11987 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.079, whole genome shotgun sequence.
ACCESSION   DANRDI010000079 DANRDI010000000
VERSION     DANRDI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.079"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            151..789
                     /locus_tag="PZ377_001637"
     CDS             151..789
                     /locus_tag="PZ377_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDM6358509.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            808..1065
                     /locus_tag="PZ377_001638"
     CDS             808..1065
                     /locus_tag="PZ377_001638"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358510.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            1044..1784
                     /locus_tag="PZ377_001639"
     CDS             1044..1784
                     /locus_tag="PZ377_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDM6358511.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(2042..2695)
                     /gene="nreC"
                     /locus_tag="PZ377_001640"
     CDS             complement(2042..2695)
                     /gene="nreC"
                     /locus_tag="PZ377_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDM6358512.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(2718..3752)
                     /gene="nreB"
                     /locus_tag="PZ377_001641"
     CDS             complement(2718..3752)
                     /gene="nreB"
                     /locus_tag="PZ377_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDM6358513.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(3776..4228)
                     /gene="nreA"
                     /locus_tag="PZ377_001642"
     CDS             complement(3776..4228)
                     /gene="nreA"
                     /locus_tag="PZ377_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDM6358514.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(4248..4925)
                     /gene="narI"
                     /locus_tag="PZ377_001643"
     CDS             complement(4248..4925)
                     /gene="narI"
                     /locus_tag="PZ377_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDM6358515.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(4918..5493)
                     /gene="narJ"
                     /locus_tag="PZ377_001644"
     CDS             complement(4918..5493)
                     /gene="narJ"
                     /locus_tag="PZ377_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDM6358516.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(5486..7045)
                     /gene="narH"
                     /locus_tag="PZ377_001645"
     CDS             complement(5486..7045)
                     /gene="narH"
                     /locus_tag="PZ377_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDM6358517.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(7035..10724)
                     /locus_tag="PZ377_001646"
     CDS             complement(7035..10724)
                     /locus_tag="PZ377_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDM6358518.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(11033..>11987)
                     /gene="cobA"
                     /locus_tag="PZ377_001647"
     CDS             complement(11033..>11987)
                     /gene="cobA"
                     /locus_tag="PZ377_001647"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDM6358519.1"
                     /translation="VYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANL
                     TTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQEEVDTLNE
                     HHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVDVNSLVNGG
                     TLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIISKLQHYSI
                     EAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEGYGCLLNPN
                     DTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
BASE COUNT         3684 a         2393 c         1792 g         4118 t
ORIGIN      
        1 agatgctttt agacacttat tcgccatcat tttaatcata ttatttagga acactcacat
       61 acttttctta atttttttca attagttaca attactgctt gaaaatcacg ttacgttatc
      121 ctttatgctg acaacataag gaggtttatc atgtataaaa tcaatattat tagatcagat
      181 tccgtctata ataatatact caaggcacca ataaacgacc gtgattcaat tttcactaaa
      241 gaaatattag taccttttaa aaagaaattt gaggtacaac atatgcctat ttataatgat
      301 gacaaacaga caatgagtgc gattcaattt ttagatgcct ttcaaatttc tcccaaagat
      361 ttacgcatgt ctgaccaaat gtcaatacaa tacctgaata atgatttctg gagtaattgc
      421 gaaaaatatt taaaagttgc tatagatcaa ttttcaaatt atagtatctc atctcaagtt
      481 tctaactatc atttcacagt attacttgga gatcgccaaa aaccacttat gtatctaaat
      541 aaaaatcgcg gtggtgatgg tggcatacca ggttatatta tgatttattt agtgccgagt
      601 acaagtacaa ttaattctat gaaaagctta attgcacatg aagtaaatca taatatgcgc
      661 tatcaatata ttgattggga tggcggaagt ttgattgaat tgattattgc agaaggatta
      721 gctgaaaact atgtagaatc attgtatggt aaagcgcata ttggaccgtg ggtaacaaat
      781 actaattgaa gtcgtgataa cgtaaaaatt aaaaatacta tttataatca tttacattta
      841 aagcatatat ttgaatcgat gccttatctc tatggtgatg atattaataa acttcaaggt
      901 aggcctatcg ttggcttatc tcatgctgcc gggtatgcat gtggctatca cttggtaaaa
      961 tactttttac aaaaaacaaa catacctatt gaagttgcta caacacttcc agcacaaaaa
     1021 ataataaatg aggtgactga attttggcat acacatacac tttaaaagat attattgaaa
     1081 ttacaggtgt aactaaaaga actttacatt attacgatga aataggatta ttagttccag
     1141 ataaaaatga taaaaattat cgcgtttata aacagcaaga cttagaaaaa ttacaaaaga
     1201 ttttaatact caagtctttt gattttgata tcgctaaaat aaaacaatac atttcgtatg
     1261 ataatgaaca attgcgaaag ttattatcag agcaaataag caagttagat aaaaagattt
     1321 ctgacttaca attaattaga cgctctgtat gtgaatttat taaaggactc tctctaatag
     1381 ataccagcat tttaaacaag acactacagt cacaatatga taaagaagca tctataaaat
     1441 atggtcatac gaaagcatat caatcattta ttagacgtaa agacagctta caatcgcagg
     1501 atatcagaca taaattgaca actatcttca ataaatttaa tcatatgtct ttgagtcatt
     1561 atccaatcca agattgtagt gatctcgtat ttgagtggaa ggcatttatg aacactatcg
     1621 ctgattttga tgatgaaaca ttatgctgta ttgctaaaac atatgaagat gatacgcgtt
     1681 tcaaagatta ctttaattca tatgataatc aaaatttagc atcatacatt tcagaagctg
     1741 ttaattattt tttgagcaat gtgaataaga gcgacaattt ttaatcctca tacagatgct
     1801 acaacactga atacaccaac taaaataatt gttcaaagta gaaaactaat catttattct
     1861 ctaaatcaat aactatatta aaagttatac ctttgcaaag cgaattagta taggttaccg
     1921 aaaggagaaa ggattaggtt ccattcgatt tataaaaaaa tatatttcga aacatacaaa
     1981 agcgccagaa gataatcatt tcctctcgcg cttttatact gaattatatt aatttcaaac
     2041 tctaaaactc taataatttc tttttcaatg catattcaac tagctcaggt ttactcttta
     2101 agccaagctt tgtcataata tgcgtcttat gtgcttctac tgttttcaca gatacaaata
     2161 atttctctgc aatttcttta ttcccgtaac ctttggcaat aagtggcaat atttctagtt
     2221 ctcgttttga taagatttta aaaggatctg ttgtgtttgc agtgtcctga tttgaattag
     2281 atacaaattc attcactaaa gatgttgtaa gtttcatatc tacatatgtt tcacctttat
     2341 atacagttcg aatagctaac aataattgtt catcaggtgc atttttcaat atgtaacctt
     2401 tcgcaccatt acgcaacaca tggaacaaat actcctcatc atcaaacatt gttaatatta
     2461 gtattttagt ttcaggaaaa ctgtcagcaa ttttactcgt agcgataaga cctgactcac
     2521 ctggtggcat acttaaatcc attagtaaca catcaggttt atattccatt actttttggt
     2581 aagcttcgac gccatctgca gccgttgcaa caacttccat atcattttga taatttaaaa
     2641 tcatagagaa ccccgtacgg acaacagcgt gatcatcggc aatgactatt ttcaatttta
     2701 ttcccccaat gtatgtttca aattggaatg ttcaatgtaa cattggtacc ctcaccaatt
     2761 ttcgtttcaa tattgacgct accgctgact aactcagctc gctcattcat tccatataaa
     2821 ccgagtccag aacctttagg cttagaactt ggatcaaaac catttcccgc atctatcact
     2881 tctgctacca aatggcgccc agtttgacgg atacctacat ttatttcatt tacatcagcg
     2941 tatttcaacg catttaaaat agcttcttgc actactcgat aaacaaccgt ttcaatatca
     3001 ctatcaaagc gagtattttt aatatttgat gtatatatga tttttattcc ataattttct
     3061 tcaaactgtt taaaatatga tttaaaagct gcttcaaggc ctagatcatc caaagaagcg
     3121 ggtcttaatt caaccgacat attacgtata tcatcaatta atttagcgac aatatattca
     3181 atattttctg cgtcttccaa aagcttagtt gtatcttctt gatattttaa taatctcaat
     3241 tgaacatcta cattgagcat ttcttgaatc acactatcat gtaactctct agaaattcgc
     3301 tttctttcat tttcttgggc tgagattgtt ttacgcatca tacgttgttg atgcaatttc
     3361 tcttgctgtt caatttgtga tgaaacattt tgaagcgtaa atgcatgaat tcccctgtct
     3421 tgatcaatca actgatatgt tgctgtaaat ggcatcactt tttgatcttt cgtcttcata
     3481 aatacttgga aattcgtagc ttgtacttgc atcgattcta agaaacagtc tttacatgat
     3541 tgtacatcat aggcatttgt atacccttca catctgtgac aaatcgcatt cgccacagca
     3601 ctgtaattat cttcttcaga taatatatct ttagcagcat cattcattgc aataatttta
     3661 ccgttatcat cagcaaaaac tatcttttcg attgaatgct cataatattt tttcaataaa
     3721 gtatctaact gtatactgtc ctcattaatc atgacttaca ccctaattca tctcattatt
     3781 tatcatcatt gaaaatacca aacttacgtt gaatatcatc attatcaaat atttttggta
     3841 aaggacgacc atctctttga ccaaataata gtacgccata cacttgattc ttataccaaa
     3901 gcggcactgc taaaactgct gttaatgatt cgctcaataa aattggatag tcaatctttt
     3961 cttcaggccc taaagctaaa ccaacattgg ctattaccat acgctttcct gttttcataa
     4021 cagttccagc taatccacga ccttttctta aaataatcaa tttaaatcga ttatttttat
     4081 tacctgaaac atagtgccat tttattggag atgatggttt gttagattca tagaaagcga
     4141 ttgccgcaaa atcataaccc tcttctttgc gtattttatc taatgtctct tgaaatctac
     4201 gatcttcaat tattgcttct ggtgtcaaat cctttcacct cttatgctta cactttattc
     4261 ttacggtaaa taatatatct gcgatttata tatgtcaaag gtacactcca aacatgcacc
     4321 aaacgtgtaa atggccaaca agccataata gtgaaaccta acaatatatg cattttaaat
     4381 gcaatcggca caccactcat caatgacgca tctggtttta acataaataa ttgtctaaac
     4441 caaattgata atgaagttct gtagttaaag tctggatgtt gtatatttgt tactaatgtt
     4501 gcgtaacatc ccataaatac gataagtaat aataagaaat ttacaaatat atccgacgct
     4561 gaacttaatc ttcgaatact tttcgtagta acacgtctcg ctgttaataa aaacatccct
     4621 atcaaagtta ttataccaaa gatgctacca atataaacag cgcctatatg atataaatgc
     4681 tcagacacac ccactgcatc catccatggt ttcggtatta acaatccaac tacgtgtcca
     4741 aaaaacactg gaataatacc taagtgaaat aataaacttc cccacatcaa cctttttctt
     4801 tctattaatt cactagattt agctgtccaa gaaaatttat cataacgata acgtgcaata
     4861 tgacctgcga caaagacaac taaacataaa tacggaaata taacccataa aaactgatta
     4921 agcatgatgt ttcactcctt ttggtgatgt caaacataat ttcaatgttt ttctaagtgc
     4981 ttgaatcaca taggcatatg gattgttatc ttcaccaagt gcattcgcca tcacatatgt
     5041 tccatcctca ataatcataa tgattaattg aatattctct tcagctcttg gatcatttcg
     5101 ccattctgcc acttgcaaaa attgaagcat caacggtaga taatcagaaa gttcattatc
     5161 taccatttct agtccaaaca tttcatataa tacctttaat ttagctaaca tttgcccacg
     5221 ttctttttgc gtatcaaatt tgttatacgt catatataat ggtgcttttt tcgtaaaatc
     5281 aaatgtatct gtataaatcg ctttgatttc tgataatgaa aatgtcatca ttacatttct
     5341 aaatgctact aaatcttcat atgctggatg atctttcgaa attgtttctt caaaagtttt
     5401 tggatgaaaa gttaattttt ctggaaaaca taactgttgt gccatatatc caaaactttc
     5461 ttgatatttt ttaaaattat cgaaattaat cacggaaaat ccctccatag aaattctcat
     5521 tataaatttc ttgaccagtt ttccctgaac ctactgcaac gccacagcct tcacagttat
     5581 ctccaaaatg ctcgccgccg taattgtatc ctgtactacc ttgtgcgtga tacgtatcta
     5641 aataggtttc tttgtgtgat gttggaataa caaatcgatc ttcatatttg gctagtccta
     5701 ataaacgata catgtcttta gtttggcgct cggttatacc taatcgctct aatcgagacg
     5761 tgtcaaatgg ctgttgagta acttgagatc tcatataact tctcatcatt gccatacgtt
     5821 gtagggctcc ttttactggc tctgtatctc ctgcagtgaa aatattagct aagtattcaa
     5881 taggtaaacg catttcttca atggctggga aaatcgcatc tggattttga gttgtatttt
     5941 taccttcaaa atagctcata attgggctaa gtggtgggca ataccaaacc atcggcatcg
     6001 ttctaaattc aggatgtaac ggaaatgcaa gtttatattc aattgctaac ttataaattg
     6061 gagagttttg tgcagcttca atccaatcgt aaccaatacc atctttttca gcttgagcaa
     6121 tgacttcttc gtcaaatggg tttaagaata tatctaattg tttttcatat aaatctttct
     6181 cgtctactgc tgaagctgct tcatgaactc gatctgcatc atataataaa acacctaagt
     6241 aacgcatacg tcctgtacaa gtttcagagc ataccgtagg catacccgcc tcgattctcg
     6301 ggaaacagaa agtacacttt tcagctttgt tcgttttcca attgaagtaa actttcttat
     6361 atggacaacc tgtcatacag taacgccatc cacgacatgc gtcttggtca actaatacaa
     6421 tgccatcttc atcacgttta tacatagcac ctgaaggaca cgatgcaacg caacttggat
     6481 tcaagcaatg ttcacataaa cgtggtaaat acatcataaa agtttcgtca aattggaatt
     6541 taatatcttc ttctattttt tggatgttag gatcttttgg acctgtaaca tgaccacctg
     6601 ctaagtcatc ttcccagtta ggtccccatt caatttcaat gttatccccc gtaatttctg
     6661 aatacgctct agcaactggc gaatgcttcc ctgatttcgc agttgttaaa tgttcataat
     6721 tatagttcca tggctcataa taatctttaa ttaatggcat atctgggtta taaaaaattt
     6781 tacctaaagc aatttttgaa attctacttc cagattttaa ttcaagtttc cctttacgat
     6841 ttagtaccca accacctttg tagtgttctt ggtcttccca acgtttcgga taccctacac
     6901 ctggcttcgt ttctacgttg ttgaaccaca tgtactcagc acctggacga tttgtccaag
     6961 tgtttttaca tgtcacacta cacgtatggc atcctatgca tttatctaaa tttaatacca
     7021 tcgcaacttg cgctttaatc ttcaagccaa ttaacctcct tcatctttct aactgctaca
     7081 tataaatccc tttggttccc aattggtcca taataattaa agtgataact aatttgtgcg
     7141 tatcctccga ctagttgtgt tggtttcaaa tggattctag tcggcgcgtt gtgtgaacca
     7201 ccacgtgtat ctgtaatttc tgacccaggc gtttgaatat gtttatcttg tgcatgatac
     7261 ataaacattg tacctttagg catacgatgc gaaataactg ctcttgccgt tacaacacca
     7321 ttacggttat acacttctag ccaatcatta tcttggatat cgtgtttttc agcatcttca
     7381 tttgatatcc aaaccgttgg accacctcta aatagtgtca acatatgctt attatcttga
     7441 tacattgagt gtatattcca ttttccatga ggcgttaaat aacgcagtac caaagcatct
     7501 gtaccacctt taattttctt atctctattc ccaaatacca ttggcggcaa tgtcggttta
     7561 tatactggta agctctcccc aaattgttgg aaaacttcgt gatccacata ataactttga
     7621 cgtcctgtta atgttctaaa aggtactaga cgttctatat tcgttgtaaa tggtgaatat
     7681 cgtcgacctt gtttatttga acctgggaat actgctgtcg gtattacttc tcgtggttgt
     7741 gaagttatat ttaaaaacga aattttctca gcagcgcgtt cgctagaaat atcttttaac
     7801 ggcattccag tttgttcttc gagatcttca tatgattttt gtgataattt accattcgta
     7861 gcagatgaaa tacttagtat tgcatcagct acattacgtg ctgtatcaat acgtggacga
     7921 ttcgctctca cagaatcatc atttgtatca ctccacgtac ctaacatact ttttaattct
     7981 tcatattgtt cactgacacc gaaacttaca ccatgtgctc caactttccc tttttcaagt
     8041 acaggaccaa gcgtgacata tttgtcgtaa attttagtgt agtcgcgttc tacaattgca
     8101 aagttaggca ttgtacgtcc aggtaccgct tcaatttcac ccttcgacca atctttcact
     8161 acgccgtatg gtgttgaaat ttcttgcttt gtatcatgac taagtggagt tgtcacaaca
     8221 tctttaaacg ttccaggtaa atagtctttt gccatttctg aaaatgcttt tgccaacgtt
     8281 ttataaatat cccagtctga acgcgattcc cataacggat caatggcagg attgaaagga
     8341 tgtacatatg gatgcatatc cgttgatgat aaatcatgtt tttcatacca agtcgctgcc
     8401 ggcaaaacaa tgtcagaata taacggtgtt gccgtcattc tgaagtctaa agagaccact
     8461 aaatctaact tacctgttgt ttcttcacgc cacgtaattt cttctggctt ttcatcttca
     8521 tttggtgtag ctaataaccc tgattttgtg ccaagtaaat gcttcataaa gtattcttga
     8581 ccttttgcag aacttgaaat taagtttgaa cgccatataa ataatgattt tggatgattc
     8641 tttttcaaat caggatcttc tattgcaaat tgtgtttgtt ttgatttcac ttcatcaatt
     8701 gcacgttgca aaatcgcttc atttgaatct ataccttcat ctttagcttc ttctgcaaac
     8761 aacaaactat ttttattaaa ttgtggatat gatggtaacc aaccaagtct agctgctaaa
     8821 acattataat cagctggatg ttgatgcttt aactcctctg ttttagctaa tggagatttt
     8881 aaacgatcta catttgactc ttcatatttc cattggtctg ttgcaaaata gaaccaactt
     8941 gttccgtttt gcaaacgtgg tggtccttgc cagtctttcg caaatgcgac agtactccat
     9001 ccttcaatcg gacgacattt ttcttgtccc acatagtgag cccaaccgcc accgttcaca
     9061 ccttgacagc cacataacat aactaagttt aagattgaac gataaatcgt atctgagtta
     9121 aaccaatggt taatacccgc acccatgata atcattgaac gcccttcagt atcgatagcg
     9181 ttttgcgcaa attctttcgc tacttgaatg acaacacttt gttttacgcc tgaaatggct
     9241 tcttgccaag caggtgtata ttttgattct gcatcgtcgt atccttttga ttctaattta
     9301 tgatcaaaac gacgcacgcc atattgactt gccattaagt caaaaattgt agcaatacgg
     9361 actttgtcac catttgctaa agtgacttgt cgagttggaa ttggacgatt gaatatccca
     9421 tctccatcac tatcaaagta tgggaattga attgtttcta attcgtatcc accttctgtc
     9481 attgataatg tagggttaat tttagaacca tcttctgttt ctagttttaa gttccacttc
     9541 ttaccttctt cccaacgttg acccattgtg ccattaggta ctactaaact atcgctgatt
     9601 gcatcatgaa taactggctt ccattcgcct tgctctgttg tttgacctaa gtcactcgct
     9661 cttaaaaatc gacccgcttt atatccattt tcatcttcat ccagcatgat aagaaacggc
     9721 atatctgtat attgtttagc gtaatttata aagcgttcat taggttgatt aacataatgt
     9781 tcttgtaaaa taacatgcgt cattgcttgt gcaattgcag catctgaacc aggattcggt
     9841 gctagccagt tatctgcaaa tttcacattt tctgcgtaat ctggtgctac tgaaatgact
     9901 tttgtacctt tatagcggac ttcagtcata aaatgtgcat ccggagtacg tgttaaaggt
     9961 acattagagc cccacataat aatgtatgat gcgttatacc agtcacttga ttcaggcaca
    10021 tctgtttgct ctccccaaat ttgtggagag gcaggtggta aatctgcata ccagtcataa
    10081 aaactaagca tttcaccacc aagcaaattg atgaatcgag cacctgctgc ataactaatc
    10141 attgacatcg ctggaatagg tgtaaatcct gcgattcgat ctggaccata tttttttatt
    10201 gtatacagta attgtgctgc gattatctct gtaacgtctt tccaatttga acgcacgtgc
    10261 cctcccatac ctcgggcttg cttatattgt ttggctttgt cttcattttc aacaatagac
    10321 gcccatgcag caacgcgatt accattgttt tcttctaatg cttcagtcca taaatcccag
    10381 agttttccac gaatatatgg atatttgatt cgaagcggac tgtattcata ccaagagaat
    10441 gacgcacctc gtggacatcc tctcggttca tattcaggca tatccggacc acaacttgga
    10501 tagtcagttt gttgattttc ccaggtaatc acaccatttt tcacaaatac tttccaagaa
    10561 catgagcctg tacagttaac accatgtgtt gttcttactt ctttatcgtg gctccaacgt
    10621 tctctgtaca ttttttccca ttctctactt ttactttcta ggatcgacca attcccatta
    10681 aatttttctg ttggcttaaa gaaattcaat ccaaattttc ccatatttat atcctcctac
    10741 gtataaaaat acgatgtgta gatgtcgtgt ttttaaatac tttaaaatgc ccaagactat
    10801 tgctttaatt agattgtaca ttttttcaca aacataaaat attagggaat cacctaatta
    10861 cttaaggaat ttccctatca ataacgggat ttcattgaaa taatacacaa tcatgtatgg
    10921 tcatgcttat tgccaatcta aatcgttcaa atttggcaca acgacaaata aggcttcaac
    10981 acgaatatat tctctcggtt gaaaccttac ttattcattt attttttata aattagtgac
    11041 ataacactgt attagcatct gcacgatcgg ttgaaatata tgttacattt tcttgctgct
    11101 taataaatgc atcatagtaa tcatattgcg acgaatgata tgtgccattc gatgtatcat
    11161 ttgggtttag caaacagcca taaccttcgt catataaatg ttcacagagc ataagggcgt
    11221 catgtttaga accacttact acataaaatt gcttcatagg atcatatgat ttaggagtgt
    11281 tttcagtata atcaacaact tcccctataa tacatatacc tggtttcgcc tcaattgaat
    11341 agtgttgcaa ttttgaaata atattactta aacgcccctt aacaacaaac tcgttaaaac
    11401 acgatgcttg aaagacaatc gctatcgggt aatcaatatc tgtgtattgt tgtatctgtg
    11461 tgataatttt ccctaaacgt tttaccccca tataaattgc taacgtgcca ccattcacta
    11521 aggaattgac atccacttca ttttcttctg aatctttaaa gtgacctgta gaaaatgtca
    11581 cacttttagc aactgtacgc attgtcaaac ctgtctgcat agtagcaact gctgcgctcg
    11641 ctgatgtcac ccctggtaca atttcaaacg caatatgatg ttcatttagt gtgtcgactt
    11701 cttcttgcac acgaccaaat atcgctggat cgccaccttt aagtctaaca accttgttat
    11761 atcgacgcgc tgcttccacg atacagtcat ttattttttc ttgctgaata tgttttgcat
    11821 acggcttttt accaacatcg ataatttcag tagtcaaatt cgcatattgt aaaattaacg
    11881 gattcactaa tcgatcatat agaatgacat ccgcttcacg tattaaacgc tcagcctttt
    11941 tcgtcaaata attcggatta cctggacccg cacctatcaa gtaaacc
//
LOCUS       DANRDI010000080        11840 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.080, whole genome shotgun sequence.
ACCESSION   DANRDI010000080 DANRDI010000000
VERSION     DANRDI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11840
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.080"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(248..3094)
                     /gene="uvrA"
                     /locus_tag="PZ377_001648"
     CDS             complement(248..3094)
                     /gene="uvrA"
                     /locus_tag="PZ377_001648"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDM6358520.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            complement(3102..5087)
                     /gene="uvrB"
                     /locus_tag="PZ377_001649"
     CDS             complement(3102..5087)
                     /gene="uvrB"
                     /locus_tag="PZ377_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDM6358521.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            complement(5356..5592)
                     /locus_tag="PZ377_001650"
     CDS             complement(5356..5592)
                     /locus_tag="PZ377_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HDM6358522.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            complement(5589..6239)
                     /locus_tag="PZ377_001651"
     CDS             complement(5589..6239)
                     /locus_tag="PZ377_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDM6358523.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            complement(6413..7252)
                     /locus_tag="PZ377_001652"
     CDS             complement(6413..7252)
                     /locus_tag="PZ377_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDM6358524.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(7400..7507)
                     /locus_tag="PZ377_001653"
     CDS             complement(7400..7507)
                     /locus_tag="PZ377_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358525.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            complement(7661..8771)
                     /gene="prfB"
                     /locus_tag="PZ377_001654"
     CDS             complement(join(7661..8698,8700..8771))
                     /gene="prfB"
                     /locus_tag="PZ377_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDM6358526.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(8940..9044)
                     /locus_tag="PZ377_001655"
     CDS             complement(8940..9044)
                     /locus_tag="PZ377_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358527.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            complement(9086..11617)
                     /gene="secA"
                     /locus_tag="PZ377_001656"
     CDS             complement(9086..11617)
                     /gene="secA"
                     /locus_tag="PZ377_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDM6358528.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
BASE COUNT         3487 a         2335 c         1636 g         4382 t
ORIGIN      
        1 cactcatgca ttcctatttt taagtacaca atagctgtgg ctaatgacaa agaaccacta
       61 cataataaat cattagtgat tctttatcat ttctgtccca ctcccagttc gatttatgtt
      121 taactactct agattgaccc tatcttaaca taattctttt tacgcatctt ttctttctac
      181 tattcattac taaattaata aaaagaagcg aaggataaat ttataacatc cttcacttct
      241 tttaatctta tttatcttca gtattttgtt tatctcgttc aagtacttct tttaaatact
      301 ttcctgtata tgatgacttt gtctgagcaa tatcttcggg tgtgccagtc gcaacaatag
      361 taccaccgcc actaccacct tcaggaccta agtctataat atagtctgct gttttgataa
      421 catctaggtt atgttcaata attacaacag tatcaccatt ttcaactaat cggtttaata
      481 cttttaataa tctactaata tcgtcaacat gtaaccctgt tgtcggttca tctaggatat
      541 aaatagattt accagttgaa cgtttatgaa gttcagatgc aagtttcaca cgttgagcct
      601 caccacctga taacgttgta gcttgttgac ctaatgtgac gtatccaaga ccaacatcaa
      661 ctagtgtttg taacttgcgc ttaatcttag gaatattttc aaaaaattgt gttgcttctt
      721 caacagtcat ttctaaaatg tcagcaatat ttttaccttt gtaagtaacc tctagtgtct
      781 cacgattata tcgtttacca tcacacactt cacaaggaac ataaacatca ggtaaaaaat
      841 gcatttcaat tttaataata ccgtcacctt tacaagcttc acagcgtcca ccttttacat
      901 taaaactaaa acgccctttt tgatatcctc gaattttagc ttcatttgtt tgcgcaaaca
      961 catcacgtat atcatcaaac acaccagtat atgttgctgg attagagcgt ggcgttctac
     1021 ctattggtga ttgatcaata tcaataattt tatcaagttg atcaatacct tcaatcttat
     1081 cgtacaatcc tggctttact ttagatttat taattttttg agctaatgat ttgtataata
     1141 cttcatttac taatgagctt ttaccagaac ctgatacacc tgtaacaacc gtcatgattg
     1201 atagtggtat gtccacatca acccctttaa gattgttgct tctagctcca cgtatagaaa
     1261 ttttacgatc tgaagccggt ctgcgatatt caggtacttc aatacgtttc ttaccactca
     1321 agtattgtcc tgttaatgat tttttatctt tcattacctt ttgaggagta ccactagaca
     1381 caatctgccc tccatgttca ccagcaccag gacctatatc cactaagtaa tcagccgcac
     1441 gcattgtatc atcatcgtgt tcaactacaa ttaaagtatt tcctaaatct ctcatttctt
     1501 taagtgtatt aattaatcga tcattatctc tttgatgcag tccaattgat ggctcatcta
     1561 atacatataa gacaccagtc aaacgcgacc caatttgcgt tgctaatcga atacgttgtg
     1621 cttcaccacc tgaaagtgta cctgaagctc tgtttaacgt taaatattca agtcccacat
     1681 tatttaaaaa agtgagtcgg gaaataattt ctttcaatat ttgatttgca atcgcttgat
     1741 catgttctga caaatcaatg tttttataat agttcagcgc ttgactgatt gaatattcga
     1801 ctacttcacc aatatttaaa ccacctacat aaacagataa cgcttcacga ctcaatcgct
     1861 ttccatgaca agtttcgcaa ggtagttcag tcatatattt actcatcatt tcacgtgtat
     1921 attctgaagg agattcatgg aatcgtctac ttatattagg aactacaccc tcgaaaacca
     1981 ttgttcgttt tctagtacca ccttgacgtt gtgtaaatgt aaattcaatt tctttgtcac
     2041 cagaaccata caataaaata tcacgttgac gttctgttaa ctttttaaaa ggtttatcca
     2101 tattgatttt ataaacttca caaacacgtt ttaacaatgt tggataaaaa tcagaactcg
     2161 tcggtatcca aggttctatt gcaccttcgt ttagcgtctt atctttgtcg ggaacaacca
     2221 agtctacatc gactgttaac ttttggccta agccatcaca tgtcggacaa gcaccaaaag
     2281 gactgttaaa gctaaacatt cttggttcta actctccgat tgaaaatcca catataggac
     2341 aagcatggct ttctgaaaac ttaaggtctt ccccgtcaat gacatcgact gttaattgtc
     2401 cttctgaaag ctctaaggca gtttctatag agtcagctag tcgtgtttca attccatctt
     2461 taacaactaa tcggtctaca acaacttcta ttgtatgatt cttgttctta tctaaagtag
     2521 gtacatcatt tacatcaaca atttcgccat cgattcttaa acgtacataa ccttttttac
     2581 caatatcttc gattagcttt tcatgactac ctttacgatg agcgatgaca ggtgctaata
     2641 attgaatctt tgtacgtgcc tctaattcca taatgcggtc aaccatttgt tgtactgttt
     2701 gcgattcaat ttctatattg tgatttggac agtaaggttt accaacacgt gcatataaca
     2761 aacgtatata atcatatatt tctgttactg ttgctacagt tgatcttgga tttttacttg
     2821 ttgttttttg atctattgaa attgctggcg ataatccttc aattgtatca acatctggtt
     2881 tgtccatttg gcctaaaaat tgacgcgcat aggcacttaa tgattcaaca taacgtcgtt
     2941 gtccttcagc atatatagta tcgaatgcta atgacgattt acctgaccca gataaacctg
     3001 tcataacaat taatttattt ttaggtagtt cgatatcaat atctttcaag ttatgcgcac
     3061 gagcaccttt tactactatg gatggttctt tcatttactt gtcacccttc tgcttttaat
     3121 tcaaataaca tatctcttaa ttctgtagct ttctcgaaat ctaaatcttt cgctgcttgt
     3181 ttcatttctt tttctatatt gtcgattgtc ttttgacgtt cttttttcgt catcttctta
     3241 ggtatcacag tttgtgcttt gtcattattt tcgtcatttt caacagtagc actaattaaa
     3301 tcatgtattt ttttattaat tgttttaggt gtaataccat gtttttcatt atgtttcatc
     3361 tgtatttctc gacgacgttg tgtctcatca attgcatact tcatcgaatc agtcatttta
     3421 tcggcataca taatgacttc acctttatcg ttacgcgcag ctctacctat tgtttgaatt
     3481 aatgagcggt tagaacgtaa aaacccttct ttatctgcat ctaatatgac aactagagaa
     3541 acttctggta tatcaatacc ctctcttaat aaattaatac ctacgataac atcatatgta
     3601 cccattcgta agtctctaat tatttcaatt cgttctaatg tcttgatttc tgaatgcaga
     3661 taattaactt taatacccgc ttctttcatg tatgtggtta aatcttcact catcttttta
     3721 gtgagcgttg taacaagtac gcgttcatta cgctcaactc ttgtttgaat ttcacttaat
     3781 aaatcgtcaa tttgattttc agtaggtcta acctcaatct taggatccag taaaccagta
     3841 ggacgaataa tttgttcaac catcttatcc gtatgttcaa tttcgtatgg tccaggcgtt
     3901 gcagatacat acacaagttg ttttgtcttt tcttcaaatt cttcaaattt aagtggacgg
     3961 ttatctaatg cactcggtaa tctaaaccca tgatccacca aaacttgttt acgcgctctg
     4021 tctccgttat acatgcctcg aacttgcggt aatgtcacat gtgattcatc aatcattact
     4081 aaccaatcat cgccaaagta atccaataaa gtatatggtg tcgaacccag tggtcgcaaa
     4141 gttaaatgta cggaatagtt ttcaattcct gaacagaatc ccatctctcg catcatttct
     4201 aaatcatagt tggtacgctg ttctaacctt tgcgcttcta gtaatttatt ctcatctcgt
     4261 aattctttca atcgttcttc caattctttt tcaatacgtt caatcgcaac tttcaacttt
     4321 tcttcacgtg ttacgaagtg agaagctggg aatatcgcaa aatgttctct ttctttcaac
     4381 acttcacctg ttaggtagtt aacttctcgg atacggtcaa tctcatcgcc gaaaaactca
     4441 acccttatac aaagttcttc tttagaggct gggaatattt ccactacatc accacgcact
     4501 cgaaacgttc ctcgttggaa atcgatgtca tttcgtgtat attgcacatc tacaagtttt
     4561 ctaagtaatt cacttctatc catttccata ccaactcgaa cacttactac taaatcttta
     4621 tattcttcag gattacctaa accatatata caacttacac tagcaataat aattacatca
     4681 tcgcgttcaa ataatgcact tgtagcagaa tgtcgtagtt gatcaatttc atcattgatt
     4741 gaggcatctt tttcaataaa agtgtcagta gacggtacgt atgcctctgg ttgataataa
     4801 tcatagtaac ttacaaagta ttccaccctg ttttcaggaa aaaattcttt aaactcacta
     4861 tataattgtc ctgctaatgt tttgttatgc gcgataatta acgttggttt cccaacttct
     4921 ttaataacat tactcatcgt aaatgttttc cctgtgccag tagcacctaa taaagtttga
     4981 tgtcttttcc ccgctttaat accttccacg atttctttaa ttgcttgcgg ttggtcacct
     5041 tgaggctcaa aatcagaatg tattttaaaa ggataatgtt caaccattgt cacatatgcc
     5101 tcctctttca acgtattatt tatatttccg ctcaactctt tgctttctca ttacatattt
     5161 tagcaaagta gtcacacaaa aagcaaacgt ttgttcgtaa aaatgtcgaa caaagaaaaa
     5221 caaacatagt tgtgctaact atgcttgcct taaatcaaat cgtttatctt gttattttta
     5281 gtatgtaaat acacattata ttaatcatca ttttgtttca acaaatttgt ttgaaacatt
     5341 attttaaagt taatcttagc gatcttcatc ttgatgttta tgaaattcga gttgatctat
     5401 aattaaataa ccagctaata atgacactac atcaataaaa ataatccact cgttatggaa
     5461 atactcttta tagattgagg caccaattaa aattaatgtc agaatagtac cgacccattt
     5521 acttcttgtt attacactaa ataatactac caagacacat ggaaagaatg ctgcgctaaa
     5581 ataccatatc attcattttc ctcttttctt ttatttaaaa tgttcatggt tgtttctctt
     5641 aattctgttc taggtataaa gttttcagtc aacatttctg gaatgatatt attaataaaa
     5701 tcttgtacag atgctaaatg gtcaaattga ataattgttt ctagactcat ttcataaatt
     5761 tcgaaaaata attcttcggg attacgtttt tgtatttctc caaatgtttc ataaagcaaa
     5821 tcaattttat cagcaactga aagtatttgg ccttctaatg aatcatcttt accttcttgc
     5881 agtcgttgct tataaacatc tctatattgt aatggaattt cttcttcaat aaaggtctct
     5941 accatttctt cttcaacttg cgaaaataat ttttttaatt cactactcgc atatttaaca
     6001 ggtgttttta tatcaccagt aaacacttcg gcgaaatcat gatttaatgc tttttcatat
     6061 aagcttttcc aattaatctt tcgtccatga tattcttcaa ctgttgctag atattgtgca
     6121 attttagtta ctttaaagga gtgtgctgca acattgtgtt caaaatattt aaattttcca
     6181 ggtaatctta taagtctttc catatctgat aatcttttaa aatattgatg tacacccatt
     6241 tcaattacct cctccattaa ttaatcataa attatacttt ctttttacat atcaatcaat
     6301 taaatatcat ttaaatatct tctttatata actctgatta aatgatacca aaaaatcctc
     6361 tcaacctgtt acttaaacag gctaagagga tagtcttgtc ttgatatatt acttagtgga
     6421 tgtaattata ttttcctgga tttaaaattg ttcttgaaga tttaacatta aatccagcat
     6481 agttcatttc agaaacagta attgttccat tagggtttac agattcaaca acaccaacat
     6541 gtccatatgg accagcagct gtttggaaaa tagcgccaac ttctggtgtt ttatctactt
     6601 taaatcctgc aacttttgct gcgtaattcc agttatttgc attgccccat aaacttccta
     6661 tacttctacc taattgtgca cgacgatcga aagcataata tgtgcagttt ccataagcat
     6721 ataagtttcc tctgttagca actgatttat tgtagttatg tgcaacaggt acagttggta
     6781 ctgatttttg tacttgagca ggtttgtatg ctacattaac tgtcttagtt actgcttgct
     6841 taggtgcttg cttaactact acttttttag atgcttgttg tacaggttgt tttactacct
     6901 ttttagcttg gcttgctttt cttactggtg atttaaccgc tttagtttgt ttcactttat
     6961 tttgaggcac aagtgaaatc acgtcaccag gaaaaattaa aggtgttaca ccaggattgt
     7021 atgaatataa ttgattcaac gttaagtgat gctctaaagc aatcttatat aatgaatcgc
     7081 cagcaactac tgtataagtt gtcggtgatt gcgtttgtgc ttgaacattt gatacataat
     7141 tatgttgaac aggtgttttt acttgtgtgc catgttgttg tgcatgtgct gcattattta
     7201 aagctaaaaa agctaacact gacgaaaccg tcactgtaag agattttttc atcttgctgt
     7261 cattcctttg ctgttagtat tttaagtatg caaatactat agcacaatac attttgtcaa
     7321 aagctattgt tataacgatg taatcaaatg gttaacaata taaaaagaat acaacctttt
     7381 atcatagtgt aaaatgtatt cataccatgt aattgagaac gttttcaata attaattcaa
     7441 taccttgaaa atcgccatag gtaatattac taaatgcaca ctgcatatgt tgttttaaca
     7501 aacacaactt ttaaaaaata tattctaact ctatctaccg aattgtactt aaatattcat
     7561 aaacaaatca tattccaaaa tctaatttac aatttattta gctaccttta aaaaaaccaa
     7621 aaaccgacgc ccttttagag cctcggtttt aaaatatata ttaatcgtgc gacattgtct
     7681 gtcttaaata tgattcgata aatggtccaa tgtctccatc catcactgca tcaaccttac
     7741 ctgtttcttc gttcgtacga tgatctttca ccattgagta tggatggaaa acatatgatc
     7801 taatttggct tccccagccg atttcttttt gttcgccacg aatttcagcc atttcacgtg
     7861 cctgctcttc caattttaat tgatataatt tagactttaa cattttcata gctgcttcac
     7921 ggtttttaat ttgagaacgt tcattttggt tattaacaac tatacctgag gggtggtggg
     7981 taattcgtat tgccgattca gttttgttaa tatgctgacc acctgcacca gaagctctga
     8041 atgtatcaac tgtaatatca tccggattga tttcaatctc tatttcatca ttattaaaat
     8101 ctggaataac gtcgcatgat gcaaatgatg tatgacgacg tcctgatgaa tcaaatggag
     8161 aaattcgtac tagtcggtgt acaccttttt cagcttttaa ataaccataa gcattatgcc
     8221 ctttgatgag caatgttaca cttttaatcc ccgcttcatc cccaggtaga taatcaacag
     8281 tttcaacttt aaagcctttc ttctcacaat aacgttgata cattctaaat agcatattag
     8341 cccaatcttg agactccgtg ccacctgcac caggatgtaa ctctagaatt gcgttattgg
     8401 catcgtgagg cccatctaat aataattgca attcgtattc atccacttta gccttaaaat
     8461 taatgacctc ttgctctaag tcttctttca tttcttcatc aaattcttct tgtaataaat
     8521 cccaagtagc atccatgtca tctacttctg cttgtagtgt tttataacca ttaactattg
     8581 cttttaacgc attattttta tctataatat cttgcgcttt cgtttggtta tcccaaaaat
     8641 taggttctgc catcatttct tcatattctt gaatattagt ttctttgttc tctaagtcaa
     8701 agagaccccc taatttgtgt taaatcttga ttatacttat ctatatttcg tttgatttct
     8761 gataattcca tagcattcgc tcctatttat atttcaattc aagtcattga tttgcatctt
     8821 ttataatgct aaattttaac ataattttgt taaataacaa tgttaagaaa tataagcaca
     8881 ctgacaatta gtttatgcat ttattgttaa aaatcagtac atttatcatc gacatatgcc
     8941 taaaccgatt ttttaaaact aagtacataa caacgtttaa caacttcttc acatttttta
     9001 aagtatttaa cgcttgtaaa ataaaaagac tcctcccata acacaaacta taggtgttta
     9061 attggaagga gttattttat atcatttatt ttccatggca atttttgaat tttttaccac
     9121 taccacatgg acaatcatcg ttacgaccaa cttgatcgcc tttaacgatt ggtttcggtt
     9181 tcactttttc tttaccatct tcagctgaaa cgtgcttcgc ttcaccaaac tctgttgttt
     9241 tttcacgttc aatattatct tcaacttgta ctacagattt taaaatgaat ttacaagtat
     9301 cttcttcaat attttgcatc atgatatcaa ataattcatg accttcattt tgatagtcac
     9361 gtaatggatt ttgttgtgca taagaacgta agtgaatacc ttgacgtaat tgatccattg
     9421 tgtcgatatg atcagtccaa tggctatcaa tagaacgaag taaaatcata cgctcaaact
     9481 cattcatttg ttcttctaag atatcttttt gactttgata tgctgcttca atcttagccc
     9541 aaacgacttc gaaaatatct tcagcatctt tacctttgat atcatcctct gtaatgtcac
     9601 cttcttgtaa gaagatgtca ttaatgtagt cgatgaatgg ttgatattca ggctcgtcat
     9661 ctgctgtatt aatatagtaa ttgatactac gttgtaacgt tgaacgtagc attgcatcta
     9721 caacttgaga gctgtcttct tcatcaataa tactatttct ttcgttatag ataatttcac
     9781 gttgtttacg taatacttca tcgtattcta agatacgttt acgcgcgtcg aagttattac
     9841 cttctacacg tttttgtgct gattctacag ctcttgatac catttttgat tcaattggtg
     9901 tagagtcatc taaacctagt cggctcatca ttttctgtaa acgttcagaa ccaaaacgaa
     9961 tcattaattc atcttgtaat gataaataga agcgactatc ccctttatca ccttgacgtc
    10021 cagaacgacc acgtaactgg tcatcaatac gacgagattc atgtcgctct gtacctatta
    10081 ctgctaaacc gcctaattcc tctacgcctt cacctaattt gatatctgta ccacgaccag
    10141 ccatgttagt ggcaatagta acggcacctt tttgtccagc gcctgcaaca atttcagctt
    10201 cacgttcatg atttttcgca tttaacacat catgacggat accacgtttt ttaagtaaat
    10261 ttgaaatata ttcagaagtc tcaactgcaa cagtacctaa tagcactggt tgccctgcct
    10321 tgtgtttttc aacaacatct tctactactg catcaaattt acctttttgg ctaatgtaaa
    10381 ttaaatcaga cttatcgtta cgttgcacag gtttatttgt cggaatttga gttactgtca
    10441 tgttataaat atttctaaat tcttcttctt cagttttagc tgtacctgtc atacccgcaa
    10501 gtttattgta cattctgaaa tagttttgga atgtaataga cgccatagtt ttagattcat
    10561 tttgaatttg aacgccttcc ttcgcttcaa tagcttggtg taaaccttcc gagaaacgac
    10621 ggcctggcat tgtacgtcct gtaaattgat cgacaattaa tacttcgcca tcaacaacca
    10681 tatagtctac gtcacgttgt aatgtaacgt gcgcacgtaa agctgtgttg atatgactaa
    10741 taacatcaac attttgtaca tcatataagt tttcaacttt gaacatacgt tcagctttat
    10801 ccgcaccttg ttctgttaaa tgtacagctt tcgttttttc atcgtattta taatcttcgt
    10861 cctgttttaa cattttcgca aaaacatttg cttgtgtata aagtgacgtt gacttttcag
    10921 cttcaccaga aataattaat ggcgtacgtg cctcgtcgat taaaattgag tcaacctcat
    10981 caatgattgc aaaatgtaat ggacgcatta ctctatcttc agaataattc accatgttat
    11041 ctcgtaagta atcaaaacct agctcattat tagtactgta agtaatgtct tgtgcgtatg
    11101 cttcacgttt ttcttctgtc gtcttactgt ttaagtttaa tccgacagtc aaacctaaga
    11161 agttatataa ctcagccatt tcttcacttt gaacacttga taagtattca ttgactgtaa
    11221 taacgtgaac acctctacca gctaatgcat ttaagtatgt tggcattgtc gctgttaatg
    11281 ttttaccttc acctgttctc atctcagcga tatcaccttt atgaattgca ataccaccca
    11341 taatttgaac tttatatggt gtcatattga atacacgttt agagccttct ctaacaagtg
    11401 catatgcttc tggtaaaatt ttatctaaat aatcattttg ctttttgaca ttatcaatgt
    11461 cagctaattc tgtttggaat tgtttcgttt tattacgaat ttcttcatca gttaaaattg
    11521 ccgttttttc ttctaaagcg attactttat cagcaagttt acctaactgt ttaatttctt
    11581 tattattgcc atcaagaatt tttgataaaa atcccatttc gttcgctcct ttagctaaaa
    11641 aactgtttgg cctacaacaa tatatcttat catttatagt tagaaaatta tacttattta
    11701 ctcatttgta gaatcaatat aaatatattt atgacatact tcattcacat tctgttgtca
    11761 acaagtttat cactaataaa tatattctca atacgcaatt atacttccta ataaattata
    11821 ttataaatat tttacgattt
//
LOCUS       DANRDI010000081        11798 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.081, whole genome shotgun sequence.
ACCESSION   DANRDI010000081 DANRDI010000000
VERSION     DANRDI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.081"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            80..1417
                     /locus_tag="PZ377_001657"
     CDS             80..1417
                     /locus_tag="PZ377_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDM6358529.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1419..2036
                     /locus_tag="PZ377_001658"
     CDS             1419..2036
                     /locus_tag="PZ377_001658"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDM6358530.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2064..2393
                     /locus_tag="PZ377_001659"
     CDS             2064..2393
                     /locus_tag="PZ377_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDM6358531.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2607..3533
                     /locus_tag="PZ377_001660"
     CDS             2607..3533
                     /locus_tag="PZ377_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDM6358532.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3534..4337
                     /locus_tag="PZ377_001661"
     CDS             3534..4337
                     /locus_tag="PZ377_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDM6358533.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4354..4701
                     /gene="yabA"
                     /locus_tag="PZ377_001662"
     CDS             4354..4701
                     /gene="yabA"
                     /locus_tag="PZ377_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDM6358534.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            4975..5700
                     /locus_tag="PZ377_001663"
     CDS             4975..5700
                     /locus_tag="PZ377_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDM6358535.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5693..5941
                     /locus_tag="PZ377_001664"
     CDS             5693..5941
                     /locus_tag="PZ377_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDM6358536.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5943..6782
                     /gene="rsmI"
                     /locus_tag="PZ377_001665"
     CDS             5943..6782
                     /gene="rsmI"
                     /locus_tag="PZ377_001665"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDM6358537.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7067..9040
                     /gene="metG"
                     /locus_tag="PZ377_001666"
     CDS             7067..9040
                     /gene="metG"
                     /locus_tag="PZ377_001666"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDM6358538.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9071..9844
                     /locus_tag="PZ377_001667"
     CDS             9071..9844
                     /locus_tag="PZ377_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDM6358539.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10011..10547
                     /gene="rnmV"
                     /locus_tag="PZ377_001668"
     CDS             10011..10547
                     /gene="rnmV"
                     /locus_tag="PZ377_001668"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDM6358540.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10558..11451
                     /gene="rsmA"
                     /locus_tag="PZ377_001669"
     CDS             10558..11451
                     /gene="rsmA"
                     /locus_tag="PZ377_001669"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDM6358541.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11551..>11798
                     /locus_tag="PZ377_001670"
     CDS             11551..>11798
                     /locus_tag="PZ377_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDM6358542.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHH"
BASE COUNT         4301 a         1568 c         2341 g         3588 t
ORIGIN      
        1 tcaaagaagt gttactatat aattaatact tttgaaagta actaattcca aaacagtgta
       61 aataaaggaa gcgtatatca tgaagcaacc tattttaaat aaattagaaa gtttaaatca
      121 agaagaagcg atttctttgc atgttccggg tcataaaaat atgactatcg gtcatttatc
      181 tcaattatca atgacaatgg ataaaactga aatacctgga ttagatgatt tacatcatcc
      241 tgaagaagtc attttggaaa gtatgaagca ggtggagaaa cattcagatt atgatgctta
      301 tttcttagtg aatggcacca cttcaggaat attatctgtc atccagtctt tttcacagaa
      361 aaaaggcgat atcttaatgg caagaaatgt acataaatct gtgttacatg cgctcgatat
      421 tagccaacaa gaagggcatt ttattgaaac gcatcaaagt ccgttaacga atcattataa
      481 taaagttaat ttaagccgtt tgaataatga cggtcacaaa cttgctgtgt tgacttatcc
      541 taactattac ggtgaaacat ttaatgtaga agaggttatc aaatttttgc accaattaaa
      601 tattcctgta ctcattgacg aagcacacgg cgcgcacttt ggattgcaag gatttccaga
      661 ttctacatta aattatcaag ctgactatgt tgttcaatct tttcataaaa cgttaccagc
      721 tttaacgatg ggctcggtac tttatattca taaaaatgca ccttatagag aaactattat
      781 agaatatcta agctacttcc aaacatctag tccttcgtat ttgattatgg ctagtttaga
      841 gtcagctgcc gagttctata aaacatatga tagtaccgtg ttttttgata agagagcgca
      901 attaatcgaa tgtttggaga agaagggttt tgaaatgctt caagttgatg atccgttgaa
      961 gttgctgata aaatatgaag gttttacagg tcatgatatt caaaattggt ttatgaatgc
     1021 acatatctat ttagaattag cggacgacta tcaagcatta gcgatattgc cgttatggca
     1081 tcatgatgat acgtatttat ttgattcgct tttacgtaaa attgaagata tgattttacc
     1141 gaaaaaatca gtttctaaag ttaaacaaac acaactttta acaactgaag gtaactataa
     1201 accaaaacgc tttgaatatg ttacttggtg tgatttgaaa aaggcaaaag gtaaagttct
     1261 ggcgcgacat attgtcccgt atccgccagg gattcctatt attttcaaag gagaaacaat
     1321 aactgaaaat atgatagaat tggtaaatga atatctggaa actggaatga tagttgaagg
     1381 aattaaaaat aataaaattt tagttgagga tgaataaaat gtcagctttt ataacttttg
     1441 agggcccaga aggctctgga aaaacaactg taattaatga agtttaccat agattagtaa
     1501 aagattatga tgtcattatg actagagaac caggtggtgt tcctactggt gaagaaatac
     1561 gtaaaattgt attagaaggc aatgatatgg acattagaac tgaagcaatg ttatttgctg
     1621 catctagaag agaacatctt gtattaaagg tcataccagc tttaaaagaa ggtaaggttg
     1681 tgttgtgtga tcgctatatc gatagttcat tagcttatca aggttatgct agagggattg
     1741 gcgttgaaga agtaagagca ttaaacgaat ttgcaataaa tggattatat ccagacttga
     1801 cgatttattt aaatgttagt gctgaagtag gtcgcgaacg tattattaaa aattcaagag
     1861 atcaaaatag attagatcaa gaagatttaa agtttcacga aaaagtaatt gaaggttacc
     1921 aagaaatcat tcataatgaa tcacaacggt tcaaaagcgt taatgcagat caacctcttg
     1981 aaaatgttgt tgaagacacg tatcaaacta tcatcaaata tttagaaaag atatgatata
     2041 attgttagaa gaggtgttat aaaatgaaaa tgattatagc gatcgtacaa gatcaagata
     2101 gtcaggaact tgcagatcaa cttgttaaaa ataactttag agcaacaaaa ttggcaacaa
     2161 caggtgggtt tttaagagcg ggtaatacaa cattcttatg tggtgtcaat gatgaccgtg
     2221 tagatgaaat attgtctgtg attaatcaaa cgtgtggtaa tagagaacag ttggtttcac
     2281 ctattacacc tatgggaggc agtgcggatt cgtacattcc atatccagtt gaagttgaag
     2341 ttggcggtgc tactgtattt gttatgccag ttgatgcatt ccatcaattt taattctata
     2401 atacaatcat caattagaga tacttaaaat agtgtattaa taagttatta acaattttgg
     2461 gttgcttgac tgcgactagt tcagatgcca atagatttga tttttgtggt tctaaaaata
     2521 atcacaaatc atggtcgcta ttgttgcagt aattagttgc tctttggcaa cctttttata
     2581 taaaagcaaa agggagtttg taatgaatgg atgaacagca acaattgacg aatgcatatc
     2641 attcaaataa attatcgcat gcctatttat ttgaaggtga tgatgcacaa acgatgaaac
     2701 aagttgcgat taattttgca aagcttattt tatgtcaaac agatagtcaa tgtgaaacaa
     2761 aggttagtac atataatcat ccagacttta tgtatatatc aacaactgag aatgcaatta
     2821 agaaagaaca agttgaacaa cttgtgcgtc atatgaatca acttcctata gaaagcacaa
     2881 ataaagtgta catcattgaa gactttgaaa agttaactgt tcaaggggaa aacagtatct
     2941 tgaaatttct tgaagaacca ccggacaata cgattgctat tttattgtct acaaaacctg
     3001 agcaaatttt agacacaatc cattcaagat gtcagcatgt atatttcaag cctattgata
     3061 aagaaaagtt tataaataga ttagttgaac aaaacatgtc taagccagta gctgaaatga
     3121 ttagtactta tactacgcaa atagataatg caatggcttt aaatgaagaa tttgatttat
     3181 tagcattaag gaaatcagtt atacgttggt gtgaattgtt gcttactaat aagccaatgg
     3241 cacttatagg tattattgat ttattgaaac aggctaaaaa taaaaaactg caatctttaa
     3301 ctattgcagc tgtgaatggt ttcttcgaag atatcataca tacaaaggta aatgtagagg
     3361 ataaacaaat atatagtgat ttaaaaaatg atattgatca atatgcgcaa aagttgtcgt
     3421 ttaatcaatt aattttgatg tttgatcaac tgacggaagc acataagaaa ttgaatcaaa
     3481 atgtaaatcc aacgcttgta tttgaacaaa tcgtaattaa gggtgtgagt tagatgccaa
     3541 atgtaatagg tgttcagttt caaaaagcgg gaaaattaga atattataca cctaatgata
     3601 tacaagtaga tatagaagac tgggtagttg tcgaatctaa aagaggcata gagataggta
     3661 ttgttaaaaa tccattaatg gatattgctg aagaggatgt tgtgttacct cttaaaaata
     3721 ttattcgcat tgctgatgac aaagatattg ataaatttaa ttgtaatgaa cgagatgctg
     3781 aaaatgcatt aatactatgt aaagacattg taagagaaca aggtttggac atgcgtttag
     3841 tcaattgcga atatacatta gataaatcga aagttatttt taattttacg gcggatgatc
     3901 gtattgattt tagaaaatta gtaaaaatat tagcgcaaca tttaaaaaca cgtatcgagt
     3961 tgagacaaat tggtgtaagg gatgaagcca aattgcttgg cggtatcgga ccttgtggta
     4021 ggtcgttatg ttgttctaca tttttagggg attttgaacc agtatcgatt aagatggcta
     4081 aggatcaaaa tttatcatta aatccaacta aaatttctgg tgcatgtggt cgtttgatgt
     4141 gttgtttaaa atatgaaaat gactattatg aggaagtacg tgcacaatta cctgatattg
     4201 gtgaagcaat tgaaacgcct gatggtaacg ggaaagtagt tgctttaaat atattagaca
     4261 tttctatgca ggtgaagctt gagggacatg aacagccact tgaatataaa ttagaagaaa
     4321 tagaaactat gcattaagga ggcattatta catttggatc gcaatgaaat atttgaaaaa
     4381 ataatgcgtt tagaaatgaa tgtcaatcaa ctttcaaagg aaacttcaga attaaaggca
     4441 cttgcagttg aattagtaga agaaaatgta gcgcttcaac ttgaaaatga taatttgaaa
     4501 aaggtgttgg gcaatgatga accaactact attgatactg cgaattcaaa accagcaaaa
     4561 gctgtgaaaa agccattacc aagtaaagat aatttggcta tattgtatgg agaaggattt
     4621 catatttgta aaggcgaatt atttggaaaa catcgacatg gtgaagattg tctgttctgt
     4681 ttagaagttt taagtgatta atcaagcaca ctcaaatagt gttataatta taaatgaata
     4741 tggtttggat aagtctgaga caatgcatgt ttcaggcttt aattgtgtat aaagttttgg
     4801 tgattgcata agagatggcg gtactaaatg ttattattaa gtgtgcacgc agtatcatta
     4861 gttataaaat gtagctgtta aaagtcaaaa atacatcgaa tgtagttagg catataatat
     4921 aaaaagagtt ttcaattact caatagaaaa aggttgtctt cataggagtt aaaaatgtta
     4981 aaagagaatg aacgatttga tcaactaatc aaagaagatt ttagtattat tcaaaatgat
     5041 gatgtttttt cattttcaac ggatgctttg ttgttagggc attttacaaa acctagaaca
     5101 aaagatattg tgttggactt atgttcaggc aatggggtga tacccttgtt attgtttgcg
     5161 aaacatccac gacatataga aggtgttgag attcaaaaaa cacttgtcga tatggcgcga
     5221 cgcacatttc aattcaatga tgttgatgaa tatttaacaa tgcatcacat ggatttgaaa
     5281 aacgttacta aagtatttaa accttcacaa tatactttag taacgtgtaa tccgccttat
     5341 tttaaagaga atcagcaaca ccaacatcaa aaagaagcac ataagatagc gagacatgag
     5401 attatgtgta cacttgaaga ttgcatgatt gcagcccgtc atttattaaa agaaggtggc
     5461 aggctaaaca tggtacatcg tgcagagaga ctaatggatg tcttgtttga aatgagaaaa
     5521 gtgaatattg aacctaagaa agtcgttttt atatatagta aagtagggaa atcagcacaa
     5581 acgatagtag tagaaggtcg aaaaggtgga aatcaaggtt tagaaatcat gcccccattt
     5641 tatatttata atgaagatgg taattatagc gaagaaatga aggaagtata ttatggatag
     5701 tcattttgta tatattgtaa aatgtagtga tggaagttta tatacaggat acgctaaaga
     5761 cgttaatgca cgtgttgaaa aacataaccg aggtcaagga gccaaatata cgaaagtaag
     5821 acgtccggtg catttagttt atcaagaaat gtatgagaca aagtctgaag cattgaagcg
     5881 tgaatatgaa attaaaactt ataccagaca aaagaaattg cgattaatta aggagcgata
     5941 gtatggctgt attatattta gtgggcacac caattggtaa tttagcagat attacttata
     6001 gagcagttga tgtattgaaa cgtgttgata tgattgcttg tgaagacact agagtaacta
     6061 gtaaactgtg taatcattat gatattccaa ctccattaaa gtcatatcac gaacataaca
     6121 aggataagca gactgctttt atcattgaac agttagaatt aggtcttgac gttgcgctcg
     6181 tatctgatgc tggattgccc ttaattagtg atcctggata cgaattagta gtggcagcca
     6241 gagaagctaa tattaaagta gagaccgtgc ctggacctaa tgctgggctg acggctttga
     6301 tggctagtgg attaccttca tatgtatata catttttagg atttttgcca cgaaaagaga
     6361 aagaaaaaag tgctgtatta gagcaacgta tgcatgaaaa tagcacatta attatatacg
     6421 aatcaccgca tcgtgtgaca gatacattaa aaacaattgc aaagatagat gcaacacgac
     6481 aagtatcact agggcgtgaa ttaactaaga agttcgaaca aattgtaact gatgatgtaa
     6541 cacaattaca agcattgatt cagcaaggcg atgtaccatt gaaaggcgaa ttcgttatct
     6601 taattgaagg tgctaaagcg aacaatgaga tatcgtggtt tgatgattta tctatcaatg
     6661 agcatgttga tcattatatt caaacttcac agatgaaacc aaaacaagct attaaaaaag
     6721 ttgctgaaga acgacaactt aaaacgaatg aagtatataa tatttatcat caaataagtt
     6781 aatcacttta tcgattatat gaaattttaa acgattttat aaacgcaagc tgtaatttta
     6841 aatggtaagt tatcattttg cattgatact gataaaatga tgttgactat gataaaaaaa
     6901 tgatgacatc gacgtttttt aatgtaaaat aaatacattg aaagtaataa ataccttaac
     6961 attgaataag atgaaaatga gatgacgaga taaatgttcg cgtccgttga aatgcataga
     7021 aatcttagat attatttgaa gtgagacatt acgaggagga acagttatgg ctaaagaaac
     7081 attttatata acaaccccaa tatactatcc tagtgggaat ttacatatag gacatgcata
     7141 ttctacagtg gctggagatg ttattgcaag atataagaga atgcaaggat atgatgttcg
     7201 ctatttgact ggaacggatg aacacggtca aaaaattcaa gaaaaagctc aaaaatctgg
     7261 taagacagaa attgaatatt tggatgagat gattgctgga attaaacaat tgtgggctaa
     7321 gcttgaaatt tcaaatgatg attttatcag aacaactgaa gaacgtcata aacatgtcgt
     7381 tgagcaagtg tttgaacgtt tattaaagca aggtgatatc tatttaggtg aatatgaagg
     7441 ttggtattct gttccggatg aaacatacta tacagagtca caattagtag acccacaata
     7501 cgaaaacggt aaaattattg gtggcaaaag tccagattct ggacacgaag ttgaactagt
     7561 taaagaagaa agttatttct ttaatattag taaatataca gaccgtttat tagagttcta
     7621 tgaccaaaat ccagatttta tacaaccacc atcaagaaaa aatgaaatga ttaacaactt
     7681 cattaaacca ggacttgctg atttagctgt ttctcgtaca tcatttaact ggggtgtcca
     7741 tgttccgtct aatccaaaac atgttgttta tgtttggatt gatgcgttag ttaactatat
     7801 ttcagcatta ggctatttat cagatgatga gtcactattt aacaaatact ggccagcaga
     7861 tattcattta atggctaagg aaattgtgcg attccactca attatttggc ctattttatt
     7921 gatggcatta gacttaccgt tacctaaaaa agtctttgca catggttgga ttttgatgaa
     7981 agatggaaaa atgagtaaat ctaaaggtaa tgtcgtagac cctaatattt taattgatcg
     8041 ctatggttta gatgctacac gttattatct aatgcgtgaa ttaccatttg gttcagatgg
     8101 cgtatttaca cctgaagcat ttgttgagcg tacaaatttc gatctagcaa atgacttagg
     8161 taacttagta aaccgtacga tttctatggt taataagtac tttgatggcg aattaccagc
     8221 gtatcaaggt ccacttcatg aattagatga agaaatggaa gctatggctt tagaaacagt
     8281 gaaaagctac actgaaagca tggaaagttt gcaattttct gtggcattat ctacggtatg
     8341 gaagtttatt agtagaacga ataagtatat tgacgaaaca acgccttggg tattagctaa
     8401 ggacgatagc caaaaagata tgttaggcaa tgtaatggct cacttagttg aaaatattcg
     8461 ttatgcagct gtattattac gtccattctt aacacatgcg ccgaaagaga tttttgaaca
     8521 attgaacatt aacaatcctc aatttatgga atttagtagt ttagagcaat atggtgtgct
     8581 taatgagtca attatggtta ctgggcaacc taaacctatt ttcccaagat tggatagcga
     8641 agcggaaatt gcatatatca aagaatcaat gcaaccgcct gctactaaag aggaaaaaga
     8701 agagattcct agcaaacctc aaattgatat taaagacttt gataaagttg aaattaaggc
     8761 agcaacgatt attgatgctg aacatgttaa gaagtcagat aagcttttaa aaattcaagt
     8821 agacttagat tctgaacaaa gacaaattgt atcaggaatt gccaaattct atacaccaga
     8881 tgatattatt ggtaaaaaag tagcagttgt tactaacctg aaaccagcta aattaatggg
     8941 acaaaaatct gaaggtatga tattatctgc tgaaaaagat ggtgtattaa ccttagtaag
     9001 tttaccaagt gcaattccaa atggtgcagt gattaaataa ctgtattttt aaaaattagg
     9061 agagataatt atgttaatcg atacacatgt ccatttaaat gatgagcaat acgatgatga
     9121 tttgagtgaa gtgattacac gtgctagaga agcaggtgtt gatcgtatgt ttgtagttgg
     9181 ttttaacaaa tcgacaattg aacgcgcgat gaaattaatc gatgagtatg attttttata
     9241 tggcattatc ggttggcatc cagttgacgc aattgatttt acagaagaac acttggaatg
     9301 gattgaatct ttagctcagc atccaaaagt gattggtatt ggtgaaatgg gattagatta
     9361 tcactgggat aaatctcctg cagatgttca aaaggaagtt tttagaaagc aaattgcttt
     9421 agctaagcgt ttgaagttac caattatcat tcataaccgt gaagcaactc aagactgtat
     9481 cgatatctta ttggaggagc atgctgaaga ggtaggcggg attatgcata gctttagtgg
     9541 ttctccagaa attgcagata ttgtaactaa taagctgaat ttttatattt cattaggtgg
     9601 acctgtgaca tttaaaaatg ctaaacagcc taaagaagtt gctaagcatg tgtcaatgga
     9661 gcgtttgcta gttgaaaccg atgcaccgta tctttcgcca catccgtata gagggaagcg
     9721 aaatgaaccg gcgagagtaa ctttagtagc tgaacaaatt gctgaattaa aaggcttatc
     9781 ttatgaagaa gtgtgcgaac aaacaactaa aaatgcagag aaattgttta atttaaattc
     9841 ataaagttaa aagtgagaaa gatcaccgcc ataaatgtaa acgatgctat attcgtttaa
     9901 tatgctatgg ttctttctca cttttttaaa ttaaaatatc gtgcatgtgg aatacgtgcg
     9961 atagagatgg ttagagcttt gaaattaaga attgtaggaa ggcgttttaa atgaaaatca
    10021 atgagtttat agttgtagaa ggacgagatg atactgagcg tgttaaacga gctgttgaat
    10081 gtgatacgat tgaaacgaat ggtagtgcca tcaacgaaca aactttagaa gtaattagaa
    10141 atgctcaaca aagtcgaggc gttattgtat taacagatcc agatttccca ggagataaaa
    10201 ttagaagtac aattactgaa catgtcaaag gtgttaaaca tgcgtatatt gatagagaaa
    10261 aagctaaaaa taaaaaaggg aaaattggtg ttgaacatgc cgaattaatt gatattaaag
    10321 aagcgttaat gcatgttagt tcaccctttg atgaagctta tgaatcaatt gataaatctg
    10381 tgctaataga gttggggtta attgttggga aagatgcaag gcgccgtaga gaaattttaa
    10441 gtagaaaatt gcgaatcggc cattccaatg gtaagcagtt attgaaaaag ttaaatgcat
    10501 ttggttatac cgaagcggat gtaaggcaag ctttagaaga tgaatgagga agtgaaaatg
    10561 ttggataata aagatattgc aacaccatca agaacgcgag cgttgttaga taaatatggc
    10621 tttaatttta aaaaaagttt aggacagaac tttttgatag atgtgaatat cattaataat
    10681 atcattgatg caagtgatat tgatgcacaa actggggtga ttgaaattgg tccaggcatg
    10741 gggtcattga cagaacaatt ggccagacat gctaaaagag tattggcatt tgaaattgat
    10801 caacgtttaa tacctgtatt aaatgataca ctatcacctt atgataatgt gacggtgatt
    10861 aatgaagata ttttaaaagc gaatattaaa gaagctgttg aaaatcattt acaagattgt
    10921 gaaaaaataa tggttgttgc aaacctgccg tactatatta cgacgccaat tttattaaat
    10981 ttgatgcaac aagatatacc aattgatggc tacgtggtga tgatgcaaaa agaagtgggc
    11041 gaacgcttaa atgctgaagt aggttcaaaa gcatatggtt cgttatcaat tgtcgtacaa
    11101 tactatacag agactagtaa agtattaacg gtacctaaat ctgtatttat gccaccacct
    11161 aatgttgatt caatagttgt aaaactgatg cagagaactg aaccgttagt aacagtagat
    11221 aacgaggaag cattctttaa gttagcaaaa gcagcatttg cacaaagaag aaagacaatt
    11281 aacaataact atcaaaatta ttttaaagat ggtaaacaac acaaagaagt gattttacaa
    11341 tggttggaac aagcaggtat tgatccaaga cgtcgcggtg aaacgctatc tattcaagat
    11401 tttgctaaat tgtatgaaga aaagaaaaaa ttccctcaat tagaaaatta aatgattgac
    11461 aaagcaaagc actattgtta aaatttaaat tttgtttgac gaaaacgttg caaatatggt
    11521 attatgtaac ttgtagcgag gtggagcaat atgccaaaat caattttgga catcaaaaat
    11581 tctattgatt gtcatgtagg aaatcgtatt gtactgaaag ccaatggagg ccgtaagaaa
    11641 acaataaaac gttctggaat tttaaaagaa acatatccgt cagttttcat tgttgagtta
    11701 gatcaagaca aacacaactt tgagagagta tcttatacat acactgatgt gttaactgaa
    11761 aatgttcaag tttcatttga agaggataat catcacga
//
LOCUS       DANRDI010000082        11655 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.082, whole genome shotgun sequence.
ACCESSION   DANRDI010000082 DANRDI010000000
VERSION     DANRDI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.082"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..95)
                     /locus_tag="PZ377_001671"
     CDS             complement(<1..95)
                     /locus_tag="PZ377_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358543.1"
                     /translation="MPKTDSACKEYLNQFFGSKRYLYQDNERVAH"
     gene            complement(96..456)
                     /locus_tag="PZ377_001672"
                     /pseudo
     CDS             complement(96..456)
                     /locus_tag="PZ377_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(468..725)
                     /locus_tag="PZ377_001673"
     CDS             complement(468..725)
                     /locus_tag="PZ377_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358544.1"
                     /translation="MKLNEVFATNLRVIMARDNVSVQDLHNETGVSRSTISGYKNGKA
                     EMVNLNVLDKLADALGVNVSELFTRNHNTHKLEDWIKKVNV"
     gene            complement(726..911)
                     /locus_tag="PZ377_001674"
     CDS             complement(726..911)
                     /locus_tag="PZ377_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDM6358545.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQHYDDVDKEKDTLAKRLDDNPDE
                     LLKYDNITIRHAYIEVE"
     gene            complement(916..1320)
                     /locus_tag="PZ377_001675"
     CDS             complement(916..1320)
                     /locus_tag="PZ377_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDM6358546.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIELQP
                     KFELQPKFGKQRPITYIADFSLWKEGKLVEVIDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGQEWMVYEDLVKVRRKRKREMK"
     gene            complement(1330..1551)
                     /locus_tag="PZ377_001676"
     CDS             complement(1330..1551)
                     /locus_tag="PZ377_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HDM6358547.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNVNEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            complement(1564..1725)
                     /locus_tag="PZ377_001677"
     CDS             complement(1564..1725)
                     /locus_tag="PZ377_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358548.1"
                     /translation="MVITKQNIKEILHCRDVYAQKMIDFANGDQEKLKKLIDDKLKEK
                     EERPAIVEY"
     gene            complement(1719..2498)
                     /locus_tag="PZ377_001678"
     CDS             complement(1719..2498)
                     /locus_tag="PZ377_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDM6358549.1"
                     /translation="MKPLFSEKINESLKKYQPTHVEKGLKCERCGSEYDLYKFAPTKK
                     HPDGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVNNYKPQNEKQVHA
                     KQSAIEYVQGFSTKEPKSLIFQGSYGTGKSHLAYAIAKAVKAKGHTVAFMHIPMLMDR
                     IKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFT
                     TNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            complement(2509..3252)
                     /locus_tag="PZ377_001679"
     CDS             complement(2509..3252)
                     /locus_tag="PZ377_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDM6358550.1"
                     /translation="MTEQPSYYSIITANVRYDNRLTDSEKLLFAEITSLSNKYGYCTA
                     SNGYFATLYNVVKETISRRISNLTKFGYLKIEIIKEGNEVKQRKMYPLTQSSIPIDAK
                     INTPIDNSVNTPIDANVKENNTSINNTSNNNINRIDILSGNPTASSIPYKEIIDYLNK
                     KAGKHFKHNTAKTKDFIKARWNQDFRLEDFKKVIDIKTAEWLNTDSDKYLRPETLFGN
                     KFEGYLNQKIQPTGTDQLERMKYDESYWD"
     gene            complement(3245..4009)
                     /locus_tag="PZ377_001680"
     CDS             complement(3245..4009)
                     /locus_tag="PZ377_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDM6358551.1"
                     /translation="MVKSIFLQDGEEIFVDDEDYERVNQYIWTKSYVDNVRRIHTNPL
                     NVSLSGFVLENGFQKIKNNDFTKNNITSIGYQQRWARPTRNTSSIYKGVYLNRKTKKW
                     SAVIKIDSKSKYLGSFVDEWEAAKAYNSAVDKYWDGQGYKNHKNQNDSIFEYEYKTYK
                     DQKRRRRGKSKFKGVYLTQSGYVAQITYKRKTYHIGWSKNIYETALMFNKINFYLHGS
                     DVILNDVPMTDELKEFINNWEVPDKIKALKEGAEND"
     gene            complement(4009..4677)
                     /locus_tag="PZ377_001681"
     CDS             complement(4009..4677)
                     /locus_tag="PZ377_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358552.1"
                     /translation="MPIITSYITQDDGTTTVVISGVELGNKETLLLDNGFDVEVDVSV
                     IDPFQITGKQRRKIFALVKDIEEYTGQPMDYMRHMFIEYVRTYYGYDERISLSNCTRT
                     QASQIIEATLDWTFYNDIPLSYKTSDLLKQDKSFLYWSTVNRNCVICGKPHADLAHYE
                     AVGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYHLHDSWLKVDERLNKML
                     KGGE"
     gene            complement(4691..5119)
                     /gene="ssb"
                     /locus_tag="PZ377_001682"
     CDS             complement(4691..5119)
                     /gene="ssb"
                     /locus_tag="PZ377_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDM6358553.1"
                     /translation="MLNRTVLVGRLTKDPEYRTTPNGVSVTTFTIAVNRTFTNAQGER
                     EADFINCVTFRKQAENVNNYLSKGSLAGVDGRLQSRSYENKDGQRVFVTEVAADSVQF
                     LEPKNSNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF"
     gene            complement(5119..5742)
                     /locus_tag="PZ377_001683"
     CDS             complement(5119..5742)
                     /locus_tag="PZ377_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDM6358554.1"
                     /translation="MTEQTLFEQLNSKNVNDHTEQKNGLTYLAWSYAHQELKKIDPNY
                     TVKVHEFPHPDINTENYFVPYLATPEGYFVQVSVTVKDSTETEWLPVLDFRNKSLAKG
                     SATTFDINKAQKRCFVKASALHGLGLYIYNGEELPSASDNDITELEERINQFVNLSQE
                     KGRDATIDKTMRWLKISNINKLSQKQIAEAHQKLDAGLKQLDSEEKQ"
     gene            complement(5735..5956)
                     /locus_tag="PZ377_001684"
     CDS             complement(5735..5956)
                     /locus_tag="PZ377_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HDM6358555.1"
                     /translation="MNQTVTYIIRHRDMPIYITNKPTDNNSDVSYSTNRNRAREFNGM
                     EEASINMDYHKAIKKTVTETIEYEEVEHD"
     gene            complement(5966..6226)
                     /locus_tag="PZ377_001685"
     CDS             complement(5966..6226)
                     /locus_tag="PZ377_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDM6358556.1"
                     /translation="MYYEIGEIIRKNIHVNGFDFKLFILKGHMGISIQVKDMNNVPIK
                     HAYVVDENDLDMASDLFNQAIDEWIEENTDEQDRLINLVMRW"
     gene            complement(6323..6484)
                     /locus_tag="PZ377_001686"
     CDS             complement(6323..6484)
                     /locus_tag="PZ377_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDM6358557.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            complement(6477..6698)
                     /locus_tag="PZ377_001687"
     CDS             complement(6477..6698)
                     /locus_tag="PZ377_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358558.1"
                     /translation="MIKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLAREKFPYHGLSDERIMEALKKENE"
     gene            6769..6999
                     /locus_tag="PZ377_001688"
     CDS             6769..6999
                     /locus_tag="PZ377_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358559.1"
                     /translation="MEFNDFQNFFGELSNQAEKEFGGDSDFFRDRINKLKEDAPENVS
                     YEIIYSIALYESLKAQQDMKILNTVKYLLDRD"
     gene            complement(7203..7397)
                     /locus_tag="PZ377_001689"
     CDS             complement(7203..7397)
                     /locus_tag="PZ377_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358560.1"
                     /translation="MEDQNKKVIYYYYDEAGNRQLLSIGDLNLYLLKDIKSRFGLYKK
                     QIPDLDNLFVQIDGVEFKVL"
     gene            complement(7414..8202)
                     /locus_tag="PZ377_001690"
     CDS             complement(7414..8202)
                     /locus_tag="PZ377_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDM6358561.1"
                     /translation="MQALQTFNFKELPVRTVEIENEPYFVGKDIAEILGYARADNAIR
                     NHVDSEDKLTHQFSASGQNRNMIIINESGLYSLIFDASKQSKNEKIRETARKFKRWVT
                     SDVLPAIRKHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKAD
                     YVDEILKSTGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTE
                     SDTIPIVRSDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANVTA"
     gene            complement(8218..8436)
                     /locus_tag="PZ377_001691"
     CDS             complement(8218..8436)
                     /locus_tag="PZ377_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358562.1"
                     /translation="MQWNLIKLRKERKCTQEDLANLLNISTEGYRLKELGKHQFKNDE
                     MFIIADFFDENIGDIFLPTKYTKSKQTS"
     gene            8578..9297
                     /locus_tag="PZ377_001692"
     CDS             8578..9297
                     /locus_tag="PZ377_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358563.1"
                     /translation="MDKKELAKFIGNKIRYYRTKLNLTQDQLGEKLNTKKATISNYET
                     GYRTPKQDDLFEIAHILNISIDDLFPTRNNKKNDITSIYSKLTPPRQKNVLNYANEQL
                     DEQNKVTSIDEYKESKLVSYIACGATGAGIGEELYDDILHEEVFFKEDETPSNADFCI
                     LVNGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLIKRVDICEDYINLVSLNP
                     KYDDIKVASFSNIKVMGKVVL"
     gene            9333..10265
                     /locus_tag="PZ377_001693"
     CDS             9333..10265
                     /locus_tag="PZ377_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358564.1"
                     /translation="MEENKTLKEYLRKFLEGYKYVVENRYNYQFSSNPEAFPFMRKDD
                     YKISIFYLNQSFFEEPCIVVISNDSKLKEIYNFRNIDIKYLSKHFTSYIYDSKKYVEE
                     QSGLLDFNNYIYYTSIYYGKYIGTVILQNNLDLFFNYGKRLANDHYNTLISKSKERLI
                     NKAHDEIQPFNHLDLNSMKEIVDDITFSYQIEQGLQAYKRELYLPAAATFAVAIETFL
                     IKLKKVNKIKHKDTDSTMYTKLLGELTKEGKVNYRTKKRVEIAYSMRNIINHSQAGAV
                     AKGDCDFLLNTLKDIVDENEKILREYTKSINKTE"
     gene            complement(10245..10424)
                     /locus_tag="PZ377_001694"
     CDS             complement(10245..10424)
                     /locus_tag="PZ377_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDM6358565.1"
                     /translation="MCVKFTDAEIAYIKESVENYSSEFDIYDDEQELKLKIYEQIMLK
                     IKSEYKDTYLFRLIN"
     gene            10537..11583
                     /locus_tag="PZ377_001695"
     CDS             10537..11583
                     /locus_tag="PZ377_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDM6358566.1"
                     /translation="MPVYKDDNTGKWYFSIRYKDVYGNNKRKMKRGFERKKDAKLAES
                     EFIQNVKYGYSDNQPFEYIFFNRLKNENLSARSIEKRTTEYNTHIKERFGNIPIGKIT
                     TTQCTAFRNYLLNDAGLSVGYARSVWAGFKAVINYAKKHYKLLYDPTLSVTPIPRTKP
                     QAKFITREEFDEKVEQITNDTSRQLTKLLFYSGLRIGEALALQWKDYDKIKGEIDVNK
                     KINLSNREIEYNLKKESSKGIIPVPKLIREMLKNMYNESSKRYKYFDENYFIFGGLEP
                     IRYVTYSYHFKSVFPNLKIHHLRHSYASYLINNGVDMYLLMELMRHSNITETIQTYSH
                     LYTNKKHQAMNIFD"
BASE COUNT         3414 a         2050 c         1817 g         4374 t
ORIGIN      
        1 atatgtgcca ctcgttcgtt atcctgatac agatatctct tagatccgaa aaattggttt
       61 aagtattctt tacatgcgct atcggtttta ggcatttatg cttcctgcca tctcttaaac
      121 atttggttat aagtattatc aaaccagtac ggatcacgtg aatgtttctg tggtacatta
      181 aacaaatgtg gcttcttctt acgtagttca gcctctttac gtcgttgcct agacatttca
      241 cgctctttgc tctctcgctc catgattttg gataacacaa tttctttata ttcagctaag
      301 cgcataccat aaggtgcatg taaggcttct aacaacgccc agccacctcg tactcttttt
      361 gcaaccattc ctggagttaa accgttcttt ttttatcaat tcattttcat gttcggtaaa
      421 tttatatggt ttaccgttaa tctttacgat actcatttat tccacctcta tacatttact
      481 tttttaatcc aatcctctaa tttgtgcgtg ttgtgatttc tagtaaatag ttcacttaca
      541 ttaacaccta gagcatctgc caatttatct aatacattta agttaaccat ctcagctttt
      601 ccgtttttat atccactaat agttgatctt gatacgccag tttcattgtg caaatcttgg
      661 acacttacgt tatctctagc catgattacc cttaaattag ttgcgaatac ttcgttcaac
      721 ttcatttatt ccacctctat atatgcatgt cttattgtta tgttgtcata ctttagtaat
      781 tcgtccggat tgtcatctaa gcgctttgcc agcgtatctt tttctttatc cacatcatcg
      841 taatgctgat attcaacttc tgtaggtatt cttatatcaa tcgttgcgtt tatatatgct
      901 tgttgttgca ttagatcact tcatttctct ttttctttta cgtctgactt tcactaagtc
      961 ctcatatacc atccattctt gacctgtgta tttaggcgct ttacatatcc acgttaaatt
     1021 cacatctcta tactgatatc tgaatatctt cgctttgatg ttggcaactt cagtcgcctt
     1081 acctttaacg tctataactt caaccagttt cccttccttc cacaaagaga aatcggctat
     1141 atacgtaatc ggtctttgct tcccaaattt aggttgtagt tcgaatttcg gttgtagttc
     1201 gatacgatca tagttagtgc cattcatatt actttctaaa tattggtaat attcacattc
     1261 tactttgcta tcaaatacaa ttcctttgta ctcaactttc ttagcgttgt atttactcat
     1321 cgtccacctc taaatatcaa atatcgtcgc ttgtaatcct agttcttgct catatagaag
     1381 cccgtgagcg cctttaaatc gttttaggtc actatcagtc ataattttct tttcgtcgct
     1441 gaaatgggct cctgtgagcg aataaacctc atttacgttg tctttatact tgatgacctt
     1501 aatatcttct gtgccatctt ctcggtataa gtaatatttt tctttcggca tttttaacac
     1561 tccttaatat tcgacgattg cgggtctttc ttctttttct ttcaacttat catcaataag
     1621 ttttttaagt ttctcttggt ctccgtttgc aaaatcaatc atcttttgag catatacatc
     1681 tctacaatgt aatatttctt ttatattttg ttttgtgatt accacgcatc tcgctccctg
     1741 aaatcgtctc cgattactct tacttttctt gcattgtgtt tcattcttga attgatacgt
     1801 tgccagttca tattttgatt tagttcttta tcactaaagt tagttgtaaa gatgttgttt
     1861 ttacctactc tgttatcaac aatgctgaaa agtttgttta aagtatgttc tgtgttctct
     1921 acacccatat catctagtac aagtaaatca atatcgctta acaatctgac taactcgtct
     1981 gtagtctcta ctgcattttt gttgtatgtc gctttgatac gatccatcaa cattggtata
     2041 tgcataaaag caactgtatg ccctttagct ttaactgctt ttgcgatagc gtatgctagg
     2101 tggcttttac cagttccata tgaaccttga aatattaatg attttggttc ttttgtagag
     2161 aaaccctgta catactctat tgctgattgt ttagcgtgta cttgtttttc attttgtggc
     2221 ttgtagttgt ttactgttgc atctcttaaa gacggattaa cgtttgattg attgaatatg
     2281 ttgtttatct tccgttgctt gtttcgctta tattcctcat agatttcaca tttgcaaccg
     2341 tctttatact cgtaaccatc cgggtgtttt ttagtaggag cgaacttata taagtcgtat
     2401 tcacttccac atctctcaca tttcaatcct ttttcgacat gagtaggttg atattttttc
     2461 aaactttcgt ttatcttttc actgaatagt ggtttcataa tatcccccct aatcccaata
     2521 actttcgtcg tacttcatgc gttctaattg atccgtgcca gttggttgta ttttttgatt
     2581 gaggtacccc tcaaatttat taccaaaaag tgtttctggt ctaaggtatt tatcgctatc
     2641 cgtgtttagc cactcagctg ttttgatatc aatcaccttt ttaaaatcct ccaacctaaa
     2701 atcttgattc catcttgctt taataaaatc ttttgtttta gctgtattgt gtttaaaatg
     2761 cttgcccgct tttttgttta agtaatcgat aatttcttta tagggtatag aagatgctgt
     2821 cgggttgccc gacaatatat ctattctatt tatattgtta ttacttgtat tattaatact
     2881 tgtattattc tctttgacat ttgcgtcaat aggggtattg acagaattat caataggggt
     2941 attgattttt gcgtcaatag gtattgacga ttgcgtcaag gggtacatct tcctttgttt
     3001 aacttcatta ccttctttga taatttcaat ttttaaataa ccaaacttgg taaggtttga
     3061 aattctacga gatatagttt ctttaacgac gttgtataaa gttgcaaagt aaccattact
     3121 tgctgtgcag tatccatact tattacttaa agacgttatt tctgcaaaaa gtaatttttc
     3181 actatcagta agtcgattat catatctgac atttgccgtt attattgagt agtaacttgg
     3241 ttgttcagtc attctcagca ccttctttca gtgcttttat tttgtccggt acttcccagt
     3301 tatttatgaa ttctttaagt tcatctgtca taggtacgtc attaaggatt acgtctgaac
     3361 catgtaaata aaaattaatt ttattaaaca tgagagcagt ctcataaata ttttttgacc
     3421 atccaatatg atatgtcttt cttttataag ttatttgcgc tacataacca ctttgagtta
     3481 aatagactcc tttgaactta ctttttcctc ttctacgacg tttttggtct ttgtaagttt
     3541 tgtattcata ttcaaatata gagtcatttt gatttttatg attcttataa ccttgtccgt
     3601 cccaatattt atctactgcg ctgttgtatg ctttagctgc ctcccattca tcaacaaaac
     3661 tacctaaata tttagatttg ctatcaattt ttattacagc agaccatttt tttgtttttc
     3721 gatttaaata aacaccttta tagatactcg aagtatttct tgtaggcctt gcccatcgtt
     3781 gttgataacc aattgaagtg atgttgtttt tggtaaaatc attatttttt attttttgaa
     3841 aaccattttc taatacaaat ccacttaagc taacgttgag tggatttgtg tgaattcttc
     3901 taacgttatc tacataagat tttgtccaaa tatattgatt aaccctctca taatcttcat
     3961 catcaacaaa aatttcttct ccatcttgta aaaatatcga tttaaccatt attctcctcc
     4021 tttcagcatt ttgttgagcc tctcatcaac ttttagccat gagtcatgca agtgatattt
     4081 atcatcaaac gacttaacgc caatcgcatg ttgctcgttg tgatgttcgc gacataacgc
     4141 taatacatgt ttgtcatagt ggttcatttt gtttctgttc atgcctctgc cgactgcttc
     4201 ataatgtgcc aggtctgcgt gaggctttcc gcatattaca cagttgcggt tgacagttga
     4261 ccagtataag aacgatttat cttgtttcag caagtcgctt gttttgtagc taagtggtat
     4321 gtcattgtag aacgtccagt caagcgttgc ttcaatgatt tgacttgctt gtgttctcgt
     4381 acaattactt agtgaaatac gttcatcata gccgtagtaa gtccttacat actcgatgaa
     4441 catatgtcgc atatagtcca ttggttgacc tgtatattct tctatgtctt tgacaagcgc
     4501 gaatattttt cgtcgttgct tgccggtaat ttgaaacgga tctatgacgc ttacatcgac
     4561 ttccacatca aatccgttat caagtagtaa tgtttcttta ttgcctaatt caacacccga
     4621 gatgacaact gttgttgtac cgtcatcttg agtgatataa ctagtaatta ttggcatcta
     4681 atcattccaa tcagaacggt aagtcatcat cagtaatcgc agtggtatta tcaaaaggat
     4741 tattaccagt ttgagtttgt ctttgttgat gataattgtt gtttggttgt tggttgctat
     4801 tcttcggttc taagaattga acactgtccg ctgctacttc tgtaacaaac acacgttgcc
     4861 cgtctttgtt ttcataactg cgtgattgta aacgtccatc aacgccagcc aatgaccctt
     4921 tggataaata attatttaca ttttctgctt gttttctaaa agttacacag ttaataaagt
     4981 ctgcctcacg ttctccttga gcgttagtaa atgttctgtt aactgcgata gtgaaagtgg
     5041 taacactcac accatttggc gttgttctat attctggatc ttttgttaag cgtcctacta
     5101 atactgttct gtttaacatt attgtttctc ctcactatcc aattgtttta atcccgcatc
     5161 taatttttgg tgtgcttctg cgatttgttt ttgacttaat ttattaatgt tagatatttt
     5221 tagccatctc atcgttttat cgatagttgc atctcgccct ttttcttgag ataagttcac
     5281 gaactgattg atacgctctt ctaattctgt aatatcgttg tcacttgcac ttggtagttc
     5341 ctcgccgttg tagatatata agcctaaacc gtgtaaagcc gaagctttta caaaacatcg
     5401 tttttgcgct ttgttaatat cgaaagttgt tgcactacct ttagcaagcg atttatttct
     5461 aaagtccaat actggaagcc actcagtctc tgtactatct ttcacagtca cagatacctg
     5521 tacaaaatag ccttctggtg tagccaaata aggtacaaaa taattttctg tgttaatatc
     5581 tggatgtgga aactcgtgta cttttactgt gtagtttggg tcaatctttt tcagctcttg
     5641 gtgtgcatat gaccatgcta gataagttaa tccatttttt tgttctgtat gatcattcac
     5701 gtttttactg ttcaactgtt caaataatgt ttgttcagtc atgttctacc tcctcgtact
     5761 caatagtttc tgtcactgtt ttcttgattg ctttgtgata atccatattg atactcgctt
     5821 cttccatacc gttaaactcc ctagctctat ttctatttgt ggagtaacta acatctgaat
     5881 tgttatcagt tggtttgtta gttatataaa ttggcatatc cctatgacgg atgatataag
     5941 ttacagtctg attcatagcg acctcctacc atctcatgac taagttaatt agtctgtcct
     6001 gttcgtctgt gttctcttca atccattcat ctattgcttg gttgaataag tctgatgcca
     6061 tatctaagtc attctcatct acgacataag catgtttaat tggtacgttg ttcatatctt
     6121 taacttgtat tgatatgccc atatgacctt ttaaaatgaa tagcttaaaa tcgaatccgt
     6181 taacatgaat atttttgcgt atgatttcgc ctatttcgta atacatcttg acttcctccg
     6241 tttttcgttt tatattgaac atgaattttt tcttaagtgt tttgtttgat actgttactt
     6301 gttggcgcaa gtagcagttt ttttattctt cataaaagta ttctttataa aatatgaatg
     6361 ttgcgatact tgcgaatccc gcaattgacc acgctgtagt gaagtataga aacggcatga
     6421 gtacaatcgc taagactgtg aagcataata ctgctaatag atagctttta taaatgttac
     6481 tcattttctt ttttcaacgc ctccattatt ctctcgtctg acaagccgtg ataagggaat
     6541 ttttctctag ctaattggac tggtattctg cctcgaatcg caatgtaacc ttcgtcttca
     6601 agctctttat tcagttctct tattatttgt cctgctttgg atttagaaac agataaaatt
     6661 actgcaagtt ctttagcttg caaactattt tttatcatat ctattcctcc tttttatttt
     6721 tgtgttgtgt ataatttagt tatctcctag tgaaaggagg tgataagtat ggaatttaat
     6781 gattttcaaa atttctttgg tgaacttagt aatcaagccg aaaaagaatt cggtggtgac
     6841 agtgactttt ttagagatag aataaataag ttgaaagaag atgctcctga aaacgtatct
     6901 tacgaaatta tttattcaat agctttatac gaaagcttaa aagctcaaca agatatgaaa
     6961 attttgaata cagttaaata tcttttagat cgtgactagc aatatccaac aatgatttgc
     7021 tctgagcatt attaattttt ggataatcaa aatttctaag tttaaatctt gtgtttttct
     7081 caatctttac aaccttccac gtcacaactg ccattgtgat gaggaaggtt gttttgtata
     7141 gtgtgttcat ttgtttatgc tcctttcgtg tataatgttg tttaagaggt gcattgctcg
     7201 ggttatagta ctttaaattc aacaccgtct atttgaacga acagattatc taaatcaggg
     7261 atttgttttt tatataaacc aaatcttgat ttaatatctt ttaataaata gagattcaaa
     7321 tctccaattg ataatagttg tctattacct gcttcgtcat agtagtaata aatgactttt
     7381 ttgttttgat cttccatttg ctgcgccctc ctgttaagca gttacgttag cttcataacc
     7441 gaattcagtc atgatttcat gtattttcaa tctacctttt tgtgtccatc tagtttgtaa
     7501 aactgtgtct tctctaccgt cagagcgtac aattggtata gtgtctgatt ctgtgtaact
     7561 cttgcccatg tgttctgagt aaagcaccca ctgtttattc acttttcgtt gtaatctagc
     7621 ttcgtgtagt agtttgttta acttttgtgc tgatataccg tagtctgccg cgatttgagt
     7681 tgtagctaat gttccagttg actttaagat ttcatctaca tagtctgctt tgggttttag
     7741 ttctccgatt tcttgttgta aaagtaagtt ttgctctttt tctttcttat actcagtcaa
     7801 cactgtaatg atgtagtctg gatcttttaa tgtttgttca attacattgt ctgttgcgta
     7861 gataccgtgt ttgcgaatgg ctggtaggac gtctgatgtt acccatcgtt tgaattttcg
     7921 agcggtttct ctgatttttt cgtttttact ttgtttagaa gcgtcaaaga ttaaactgta
     7981 taatcctgat tcattgataa tgatcatatt tctgttttga cctgatgcac taaattggtg
     8041 cgtcagcttg tcctcgctat caacatgatt tctgatggca ttgtctgccc ttgcatatcc
     8101 taaaatttca gcaatatctt ttcctacaaa ataaggttcg ttttcaattt ccactgttct
     8161 tactggtagc tctttaaaat taaatgtttg taatgcttgc attgttcgtt cctcctttta
     8221 agatgtttgt ttgcttttcg tgtactttgt gggtaaaaaa atatctccaa tattttcgtc
     8281 aaaaaaatca gcgataataa acatctcatc attcttaaat tgatgctttc ctaattcctt
     8341 taaacgataa ccttcagttg atatattcaa gaggtttgct aaatcttctt gagtacactt
     8401 tctttctttt ctcaacttta ttaaattcca ttgcatgttg tcacctcccg cttacaaaac
     8461 taactataca cgatacgtgt acttgagtca acataaaagt ttgcttttcg tgtatttttt
     8521 tgttgaatac caaaaataat tgggttatac tataggtaaa tttaaggagg taagaaaatg
     8581 gataaaaaag aattagcgaa atttataggc aataaaatca gatactatag aaccaaattg
     8641 aacttaactc aagatcaact tggagaaaaa ctcaacacta aaaaggctac tatttcaaat
     8701 tatgagacag ggtacagaac tcctaaacaa gatgatttgt ttgaaattgc tcatatttta
     8761 aatatcagta tcgatgattt gtttcctaca agaaataata aaaaaaacga catcacttcc
     8821 atatacagta aactcacgcc tccaagacaa aaaaacgtac ttaactacgc aaatgagcaa
     8881 ttagatgaac agaataaagt cacttctata gatgaatata aagagtctaa actagtatcg
     8941 tatattgcat gtggtgcaac tggtgctggc ataggagaag aattatatga tgacatattg
     9001 catgaagaag tattttttaa agaagacgaa acgccatcaa atgctgattt ttgtatttta
     9061 gttaatggtg attcaatgga acctatgtta aaacaaggaa catacgcttt tattaagaaa
     9121 gaagattcta ttaaagatgg tacaattgca ctcgttgtat tagatggagt aagtcttatc
     9181 aagcgtgtag atatatgcga agactatatt aatttggtat ctctaaatcc gaagtatgat
     9241 gatatcaaag tcgcttcgtt tagtaatatt aaagtaatgg gcaaagttgt attgtgatta
     9301 ataacgccta tgtggcgcga ggaggatgag ggatggaaga gaataaaact ttaaaagaat
     9361 acttgcgtaa atttttagaa ggctacaaat atgtagttga aaacagatac aattatcagt
     9421 ttagtagcaa tccagaagct ttcccattca tgagaaaaga cgattacaag atttcgatat
     9481 tttatctaaa tcaatctttt tttgaagaac cttgcatcgt cgttatctca aatgacagta
     9541 aattaaaaga aatatataat tttcgtaata ttgatatcaa atatttgtct aaacacttta
     9601 cttcatacat atatgattct aaaaagtatg tagaagaaca atccggatta ttagatttta
     9661 ataattacat ttattacaca tctatttact acggaaaata tatcgggacc gtaatattac
     9721 aaaacaattt agatttattt tttaattatg gcaaaagatt agctaacgat cattacaata
     9781 cattgatatc gaagtcgaaa gaaagattga taaacaaagc acatgatgaa atacaaccgt
     9841 tcaaccactt agatttaaat agtatgaaag agattgttga tgatataact ttttcttatc
     9901 aaatagaaca aggattacaa gcttataaaa gggaattgta tttgccagct gcagcaacct
     9961 ttgctgttgc tatagaaacg tttttaatca aattaaaaaa agttaataaa atcaaacata
    10021 aagacaccga ttcaactatg tacacaaaat tattaggaga attaactaaa gaaggtaaag
    10081 taaattatag aaccaaaaaa cgggtagaaa ttgcgtatag tatgagaaac ataatcaacc
    10141 attcacaagc tggtgcagta gccaaaggtg attgtgactt tcttttaaac acactaaaag
    10201 acattgttga tgaaaacgaa aaaatattaa gagaatatac caaatcaatt aataagacgg
    10261 aataaatagg tatccttgta ttcagatttg atttttaaca taatttgttc ataaattttt
    10321 aatttaagtt cttgttcatc gtcataaata tcaaattcac tactataatt ttcaactgat
    10381 tcttttatat aagctatttc tgcgtcagta aattttacac acatttcatc acctactttt
    10441 tattttatta tatcacattt agtagctagt actaaaatca cgggtagccc gcctaccctt
    10501 attatttttt gccaattttg aggagggaaa agcaaaatgc cagtatataa ggatgataat
    10561 acaggtaaat ggtatttttc cattagatat aaagatgtat acggtaataa caaacgaaaa
    10621 atgaagcgtg ggtttgaacg taagaaagat gccaaactag ctgaaagcga atttatacaa
    10681 aatgttaaat atggatactc ggacaatcaa ccctttgaat atatattttt taatcgttta
    10741 aaaaatgaaa atctttctgc acgctcaata gaaaagcgaa ctacagaata taatactcac
    10801 ataaaagaaa ggttcggaaa tatccctatt ggcaaaatca ctactacgca atgtactgct
    10861 ttcaggaatt atttgttaaa cgatgcaggt ctttctgttg gctatgcacg atctgtgtgg
    10921 gcaggtttta aagcagttat caattacgcc aaaaagcatt acaagctctt atacgacccc
    10981 acattatcgg taactcctat tcccagaaca aaaccacaag ctaaatttat cactcgtgaa
    11041 gaatttgatg aaaaagtaga acaaatcaca aacgatactt ctcgtcagct aactaaactg
    11101 ttattttatt ctggtcttag aataggcgaa gctttagctt tgcagtggaa agattacgat
    11161 aaaataaaag gcgaaattga cgtaaataag aaaatcaatt taagtaatag agaaattgaa
    11221 tataatctaa aaaaagaaag ttctaaaggg ataatacctg taccaaaatt aattagagag
    11281 atgcttaaaa acatgtataa tgaatcttct aaaagatata aatattttga cgaaaactat
    11341 tttatattcg gggggttaga acctattaga tacgttacct attcgtatca ttttaaatct
    11401 gtattcccga atctaaaaat acaccattta agacactcgt acgcaagcta tttaattaat
    11461 aatggtgtag atatgtattt attaatggaa ttaatgaggc actctaacat tacagaaaca
    11521 attcaaacgt actctcattt atatactaat aaaaaacatc aagctatgaa catatttgat
    11581 taaatggtat catatcggta tcaaataacg attaaggagt ttataaaatg cgtaataaca
    11641 agcctaaaat aagaa
//
LOCUS       DANRDI010000083        11586 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.083, whole genome shotgun sequence.
ACCESSION   DANRDI010000083 DANRDI010000000
VERSION     DANRDI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.083"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            251..880
                     /locus_tag="PZ377_001696"
     CDS             251..880
                     /locus_tag="PZ377_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358567.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(1033..2706)
                     /locus_tag="PZ377_001697"
     CDS             complement(1033..2706)
                     /locus_tag="PZ377_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDM6358568.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            3023..4621
                     /locus_tag="PZ377_001698"
     CDS             3023..4621
                     /locus_tag="PZ377_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDM6358569.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            5001..6479
                     /gene="mqo"
                     /locus_tag="PZ377_001699"
     CDS             5001..6479
                     /gene="mqo"
                     /locus_tag="PZ377_001699"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDM6358570.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(6545..7000)
                     /locus_tag="PZ377_001700"
     CDS             complement(6545..7000)
                     /locus_tag="PZ377_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDM6358571.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(6997..7440)
                     /locus_tag="PZ377_001701"
     CDS             complement(6997..7440)
                     /locus_tag="PZ377_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6358572.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(7603..8976)
                     /gene="hssS"
                     /locus_tag="PZ377_001702"
     CDS             complement(7603..8976)
                     /gene="hssS"
                     /locus_tag="PZ377_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDM6358573.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            complement(8969..9643)
                     /gene="hssR"
                     /locus_tag="PZ377_001703"
     CDS             complement(8969..9643)
                     /gene="hssR"
                     /locus_tag="PZ377_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDM6358574.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            9779..10834
                     /locus_tag="PZ377_001704"
     CDS             9779..10834
                     /locus_tag="PZ377_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358575.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            10834..11499
                     /locus_tag="PZ377_001705"
     CDS             10834..11499
                     /locus_tag="PZ377_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358576.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
BASE COUNT         3988 a         1646 c         2042 g         3910 t
ORIGIN      
        1 ctaaatgcgt aatgaattgt taaagtaaaa taattaaatt tatagaaaac ataaaattca
       61 caaaaattaa ataataatga acaaaaatta gggtataata aaaagaacct tagattgtag
      121 ttatggatat tgtgataatt cagatcatat taatgttgac gtctttggta acaagccatg
      181 aaaagataca catggtagat atgtatttca agattattca atgaatatcg aattatagga
      241 ggagatatgt atgaaaagat tagttacagg gttactagca ttatcattat ttttagctgc
      301 atgtggtcaa gatagtgacc aacaaaaaga cggtaataaa gaaaaagatg ataaagcgaa
      361 aactgaacaa caagataaaa aaacaaatga ttcatctaaa gataagaaag ataataaaga
      421 tgatagtaaa gacgtaaaca aagataataa agataatagt gcaaacgata accagcaaca
      481 atctaattca aatgcaacaa acaatgacca aaaccaaaca aataataacc aatcaagtaa
      541 taaccaagcg aataataatc aaaaatcaag ttacgttgca ccatattatg gacaaaatgc
      601 cgcgccggtt gcacgtcaaa tttatccgtt taatggaaat aaaaatcaag ctttacagca
      661 attgccaaat ttccaaacag ctttaaatgc ggctaataat gaagcaaata aatttggtag
      721 taataataaa gtgtataatg attattctat tgaagaacat aatggcaact ataagtatgt
      781 gtttagtttt aaagacccaa atgcaaatgg aaaatattca attgtaacgg ttgattatac
      841 tggacaagca atggttactg atccaaacta ccaacaataa tgttaatata actatgatgc
      901 aagttaaaaa ataaaacggt aaactctata atatgaatta gggtttaccg ttttttgcgt
      961 attttaaagt atcaaagttt acgttttata gtacttttaa acctcattca cagccactga
     1021 gaaatttaaa cactattgat tagatgtcat cttagacaat ataagttgtg aaatagattc
     1081 taatggttgt tctttatgtt gtatttgtgg atatttaatc actggtattg cctgggaaat
     1141 taaattatta attaataatt gtcggtctgt ataccaaatt tttgatagat gacgccaaat
     1201 atcttcgtta acaccttgag actcaatgta ttgactgtga tttggagtgt ataatgctac
     1261 tgttttgtct atagccatcg catcaaatat tatatttgag taatcagtga taacgacatc
     1321 agacattaga attaagtctt gagcactaag atactttggt gcaacaagcg cctcttctgg
     1381 taagatcgct tcatcaacac cctgaaccac aacatgatag gctttgaata aagcatctga
     1441 tagtggtaat tgttgcgcac tcactaatcc aataggtgcg tataataaaa ctggtttttg
     1501 atcatttatt ttaaacgatt tcttatactg ttgttgagta gtactttcgt tactcttttg
     1561 tagtaaatat tggtgtttag gattaccata agccaataca tttgcttggt gactaggaaa
     1621 agccgtttga taaaagtggc taatatcatc agcagaatgt ataacataat cgaattgcaa
     1681 ccagcgatta taccttttag cacgataaaa aggtgtttct atatttttaa taggctcttt
     1741 actatctaaa aataattgca ttattggtgt gccttgatgt aattgaatta aagtaccgtt
     1801 tggttgtaac gtttcaggta tatcattttc aactataact acgcgtgcgc tgttaatcaa
     1861 ttcgtcagct ttttcagact taggtgatat gaaatgtggt ccgcgacgat cgtcagtaac
     1921 aaaataacag gcagtcattg gattatgttt aacgaagtaa gtaaataaat attttgaatt
     1981 acctatatat tgatgatgat caccgataaa aacgatgtga tcttttaaaa cgtttggttg
     2041 cttcgtatac aggccgaagt tttgtaattg tttgttatca gcacgttgct ttgctatttt
     2101 atataaccat ttaggtagca taaacttggt agaatacaac caattatctg gcgaattaag
     2161 ttgttctgaa tcctcgataa aaaggttgcg aaaaggtata ggatgatgat caatataagc
     2221 cattttattc aaataaaact gagacatcat atcaaaatct tcaatagttt taatagtttc
     2281 ccatattgaa gcacttgttg ctataggggc tttaaaatca aaaacgacat gaccatcgat
     2341 tgataaatca cctttcagaa gtgtcaataa agctaaatcg aaaatcgttt ctacagagta
     2401 ataatgtatt aaatcaagag tctgttgaat cgcatatatc aggttaggat aataattatt
     2461 gttttgagcc caatcggcaa tgttgatcga tgattgaccg aaatagtgac aatcattttt
     2521 gtattgtcca tccacagtat aatcaaccac gatagactta ggatataatt gaaccgcatc
     2581 taacatcgat tgataaatca ttatctcatt ggcatatgga acgacatgtg tataaccttc
     2641 agactgagct ttcattaatt gagattcaaa cttttgcata ttagaaacat ttatctgttt
     2701 aatcattgct ataacttcca ttcctcatcg tagtatcttc cttattttag cacggtctta
     2761 tgtaaaattc atgaatagta taagttactt cattacatta agtacaattc attttgatga
     2821 acagatttaa aatgaaaaat aaaacaatta aataggaaag tgatattgtt aatagtctga
     2881 aaaaatggta tattttaatt gtgaaaaaaa tcacattaat ctcactgttc tgtaaaatgt
     2941 tatcgataac aaaatgctat agccagagca tgattttaat gaagcaatta cactactact
     3001 ttatggaaag aagagtcagc ctatgttagt aaatacgttt aacccatttg ataatttatt
     3061 attatcgtct ttaattgcag caataccgat tgtactgttt ttactatgtt taactgtttt
     3121 taaaatgaaa ggtatttatg cagctattac aacacttgtt gtaacattac taattgcaat
     3181 accatttttc aaattgccag ttggtatcgc ttctggtgca gtagtcgaag gtttcttcca
     3241 aggtatcatt ccgattggct atatcgttat gatggcagta ttgttataca aaattactgt
     3301 tgaatctgga caatttttaa caattcaaga tagtattaca aatatttcac aagaccaacg
     3361 tattcaagtt ttacttattg gatttgcatt caacgcattt ttagaaggtg cagcaggatt
     3421 tggtgtacca attgcaattt gtgcactttt attaacacaa ttaggattta atccattaaa
     3481 agctgcgatg ttatgtttag tcgcaaatgc agcgtctggt gcttttggtg cgattggtat
     3541 ccctgtaggt gttgtagaaa cgttgaaatt acctggagat gtttcagtat taggtgtttc
     3601 tcaatcagca actttaacat tggcaatcat aaatttcatt attccattct tacttatctt
     3661 tattattgat ggttttagag gtgttaaaga aacattacca gcaattttag tagtttcaat
     3721 cacttataca tttactcaag gattattaac tgtattcagt ggacctgaat tagcagatat
     3781 tattccaccg ttattaacaa tgttagcatt agcagtattt tctaaaaaat tccaaccaaa
     3841 acacatttat cgtgttaata aagatgaaga aattgaacct gcaaaagcac attctgcaaa
     3901 agcagtatta catgcatgga gcccattcat tgtattaaca gtcattgtaa tgatttggag
     3961 tgcgccattc tttaaaaact tattcttacc aaatggtgct ttatcatcat tagtatttaa
     4021 attcaactta cctggaacaa tcagcgaagt tacgcataaa ccattagtat tgactttaaa
     4081 tattattgga caaacaggta cagctatttt attaactatt attattacaa ttttaatgtc
     4141 taaaaaggtt aactttaaag atgcaggtag attattcggc gttacattta aagagttgtg
     4201 gttaccagtt cttacaattt gtttcatctt agcaatttct aaaatcacaa cttatggtgg
     4261 tttaagtgca gcaatgggtc aaggtattgc taaagcaggt aatgtcttcc cagttctatc
     4321 accaatttta ggttggatag gtgtgtttat gacaggatca gttgtaaata acaactcatt
     4381 atttgcacca attcaagctt ctgttgcaca acaaattgga acaagtggtt cacttcttgt
     4441 atctgctaat acagttggtg gtgtagcggc aaaattgatt tcaccacaat caattgcaat
     4501 tgcaactgca gcagtaaaac aagttggtaa ggaatcagaa ttattaaaaa tgacattgaa
     4561 atacagtgta tgtttactaa tattcatctg tatttggact ttcatcttgt cattattata
     4621 aaaaaacgta tttcaaaata taaatataca gaaggtgaga tgttttctaa catctcatct
     4681 ttttttatgg atcattaatg aaagaagttt gacattataa taatggtagc gctttatgtt
     4741 aaaatgaata gtgagtaatc agcaatcaaa ttaaattggt tgatagctgt taaggtttgt
     4801 ggttttgtct ttgtgctatc gcacataaag tatataatta aagtagtttc gttattataa
     4861 aatattaata tacatagtag atagtaatag agcatcacca tgggaaccta ttgagacact
     4921 tattgattta aagtggtatt aatatgtcgt atttctcgaa cgttccatta ttcattttaa
     4981 aaagggggac tgtatttgtt atgacaacac aacatagcaa aacagatgtc atcttaattg
     5041 gtggcggtat tatgagtgca acattaggaa cattacttaa agaattatca cctgagaaaa
     5101 atattaaagt gtttgaaaaa ttagcacaac ctggcgaaga gagttcaaat gtatggaata
     5161 atgccggtac agggcattca gcactttgcg agttgaacta tacaaaagaa ggtaaggatg
     5221 gcacagttga ttgtagtaaa gcaattaaga taaatgagca gtaccaaatt tcaaaacagt
     5281 tttgggcata tttagttaaa acaggacaat tagataaccc agatcgcttt attcaagcgg
     5341 tgccacacat gagttttgtc attggcgaag ataatgtagc ttttataaaa agtcgtgttg
     5401 caacgttaaa gaaaagtatt ttattcgaaa aaatgaaatt atcgcaagat gaagaagaaa
     5461 tgaaatcttg ggtaccgtta atgattgaag gtcgtaagtc tgatgaacca attgctttaa
     5521 cttatgatga aactggtaca gatgttaact ttggtgcgtt aactgcaaag ttatttgata
     5581 atttagagca acgtggtgtg ggaattcaat ataagcagaa tgtattagac atcaagaaac
     5641 agaaatctgg ggtatggcta gttaaagtta aagatttaga aactaatgaa acgacaacat
     5701 atgaatctga ttttgtattt attggtgctg gcggtgcgag tttaccatta ctccaaaaga
     5761 ctgggattaa acaatcaaaa catattggtg gtttcccggt aagtggatta ttcctgcgct
     5821 gtacaaatca agaagtgatt gatcgtcatc atgctaaagt gtacggaaaa gcagcagtgg
     5881 gtgcgccacc aatgtcagtg ccgcacttag atacacgttt tgtagacggc aagcgttcat
     5941 tgttatttgg tccatttgca ggtttctcac ctaaattttt aaaaacaggt tcacatatgg
     6001 atttaattaa atcggttaaa ccaaataata tcgtgacgat gttatctgca ggtatcaaag
     6061 aaatgagtct tacgaagtat ttagtgtcac aattgatgtt atctaatgat gagcgtatgg
     6121 atgatttaag agtctttttc ccaaatgcta aaaatgaaga ttgggaagtg attacagcag
     6181 ggcaacgtgt ccaagtaatc aaggatactg aggattctaa aggtaactta caatttggta
     6241 ctgaagttat tacgtcagat gatggcacat tagctgcatt acttggtgca tcacctggtg
     6301 cgtcaacagc tgtagatatt atgtttgatg ttttacagag atgctatcgt gatgaattca
     6361 aaggatggga accaaagatt aaagaaatgg tgccgtcatt tggttatcgc ttaacagatc
     6421 atgaggattt atatcataaa attaatgaag aagtaactaa gtatttacaa gttaaataat
     6481 aaacgaaacg gtaatgtctt ttttaatgtg atagacatta ccgtttttta gtggttaata
     6541 aaaatcattt taattgtttc agttgcttgt taatagtgtc tacgtagttc ttgtttttaa
     6601 agaattgaat tatccaaatt aatacataaa ccacaatgaa gataattgtg aatatgatta
     6661 gataatgcac tgttagtgga aaccaaccgg caagcattgc taaaggcaag aatccgacat
     6721 acgttgttat gaaatgcatt atagttgctt tagtaatgct ccaatctgtg tatttaaaga
     6781 taaaatctcc aaggaaaaag acgacgccta tgagtaacca taaaatgata gaaatcaaca
     6841 ttacggtagt ttctgtgaaa tgcgtataat acaatatgcc aatagttgat tgtgggttca
     6901 gtggataata tttgccgtct gcaaataaca tactaaagaa cagtgaaagg gacaaaccaa
     6961 tgattaagct aataaataat gagtttttca aatttttcat attgataagc gctcctttat
     7021 agattttaaa taacgtctag aagaataggt gtagtgtgca tctttaagat acatacgtat
     7081 aagtccattt ggctctaata ataatttttc aatgtaatac ttgttgacga tttctgattt
     7141 ggaaatgcga atgaaatgtt gtggtaactg tttttctagt tcataaagtc gtaattttag
     7201 tttgaatttt tgattgcttg taatggctgt tacattttta ttaataactt ggaatgttat
     7261 aacatctgat acattaatga tatggatttc tttgtctatg tatccgacta atgtatgtga
     7321 tttgtctaaa tcattgactg cattaataat actttgaacg ttatcattca ttttaggtgc
     7381 atgtatatca atataagatt ccgtctcatt tgcattgata aataaattga gtttcatcat
     7441 aggttaatgc ctccttcaaa attattaaac cataaatgac catcgatata tttaaatttt
     7501 gttgaatggt agaaattaaa tgttaagtgg ctagaaagcg ctaatcaata taaaagatac
     7561 ctcctgaaat aaaaacagaa atgttttttc aggaggtaga gattaaagtg aattatttgg
     7621 cagtgtaata gtaaaggtgg ttacatactc gttactttgt gtgaattgga ttgtaccatg
     7681 atgcaattca atgatggatt ttgtaattgc aagacctaaa ccattgctat tatcatgttt
     7741 gctcacttta taaaaacgtt caaataaacg tgcttcagct tgtggactaa ttggtgaacc
     7801 atcattactt attgtgaaaa tgatattgtt gtgactatgt tgcaaagcga tgtcaatggc
     7861 accaccaaca tctgtatact taatagcatt tattaataaa ttactcaatg cttgatgtaa
     7921 caaacgttga tttcctagga aattgattga ttctaggtca gctaaaatga ttaacgactt
     7981 ttcatcagca gcaaattgtt catgtcgaat gatatcttta atgagttgat tgacttgaat
     8041 cttatcatcg aataataaat gttggtgatt atctaattcg gataagagca acaattcagt
     8101 tgttaatccg ctcagctgcg ttgtaatggt atagatatca tttatatatt gttgtcttaa
     8161 tgttttatca gaagtttgtt gtaactcgct taataaatga tgaatatgtg ttaatggtgt
     8221 tttgatttca tgagatacat tttgtacaaa atgctgcctc atttgatcaa cttgtcccaa
     8281 tgactctctc atcttattaa agtgatactg taatgttcca atttcatctt tgcgtgtttg
     8341 tttgataggt gtttcaaaat caccatcaat taagcgttcg gtcgctagct ttaacttttt
     8401 tactggacga atgattgaat aggttgatgc gataactaat gaaatcgaga taaataataa
     8461 caacattaac aagacggcta agaatgttct aaactcgcta aatgtttctc caatatctgg
     8521 acgcataaat actgctattg aaccgtcttt tgtcttaaag ttaataccaa cagtattatc
     8581 tgttacattg tcgaaaaatc cagttacgaa taatgcgaat ggtttatctt taataccatg
     8641 gtaatcttga ttatttaata cgttgttaat agcattttgt gataaagtat cttctctaaa
     8701 tggctcccca taaaatgtct tatgaccttt ttgatcaatg gtcattattt ggtaattcat
     8761 ttgccctaaa tgtttgaaat attgttgaat gtgcgttggt ttagctgatt gttcatattg
     8821 tcttgcttct ttaagcgtct tcatgatttt cgcgtcatta gatgctttta aattataatg
     8881 atagtaaaca tttgttaata caaaacttat taatgcacta aataaaataa ccgtaatgga
     8941 ataaatcgca attctagcat agagtgtttt aaacatgatt ctccacctta tagccttgtc
     9001 ctcttactgt ttcaattgta agtgtggcat ttaatttttt taatctttgg cgtagtcgct
     9061 taatatgaac gtcaactgtt cgctcatctc cttcataatc atagccccaa attttttcta
     9121 ttatttgttc acgagtaaag atttgcttag gacgtgctgc aagcataaat aataattgaa
     9181 attccttgtt cggtaacgtc atcgttttat tagatacttg gagttccaaa taggattggt
     9241 ttagcgttaa gttgccaata gtcatttctg aatttgaatt gatattatat cgacgtaata
     9301 cagcacgaat tctaaaaata agttccttaa cctcaaaggg tttggttaca taatcgtcag
     9361 taccgcttat aaacgcacgc tctttgtcac taagtgcatc ccgcgctgtt aacataataa
     9421 ctggtatatc ataatcattt tttaatgtat tacataattg aaagccgtcc ataccatcca
     9481 tcataatatc taccactgca atatcgacac gctgtttttc taaaagtttt aatgcagctt
     9541 caccacttgg ttgtgtgtat gcatcaatgt gctctgtttg taaatggcta gctatataat
     9601 ttaggattcg aggatcgtca tcgacaacaa gacattgcac catagctata aactccctta
     9661 tctttttcat ttattataca tgtaaaatat ttttgcgtaa aaaaacaatt gttcatattg
     9721 agttcatatt tcaaccttat actgacgcta aagaagaaat agggagaagt gaatcgatat
     9781 gaaattagcg ataaaagaga ttatgtttta caaatttcgt tatattttaa tcacattaat
     9841 cattctttta ttaagtatta tggtgttatt tattagtggt ttagctcaag ggcttggtag
     9901 ggagaatatt tcgttatttg aacactttga taatgatgaa tatgttgttc aaaaaatgaa
     9961 agagccgcaa attgagaaat cgcaacttag tgatacgcaa cagaatcaaa ttaaaaaggt
    10021 aattcatcaa gaaccatata aaatgaatat tcaaacattg aaattatcta ataaagaaca
    10081 agatgtgatt acaatgaatg acgttaaaca acaacgcata caacttaaaa aaggtgatta
    10141 tcctaaaaat gctcatgagg tcgctattaa tgataagtta gctgcagaca acattagagt
    10201 cggggataga ttacatttta aaaataattc aactagttat agagtttctg gtattttaaa
    10261 cgacacaatg tatgcgcata gttccattgt gctattgaac gataacggat ttaatgcatt
    10321 gaataaggtt aatacggcat tttatccagt gaaaaattta acacaacaac aacgtgatga
    10381 gcttaataaa ataaatgacg ttcaagttgt gagtgaaaaa gatttaacag gtaatattgc
    10441 gagttatcaa gcagagcaag caccgttaaa tatgatgatt gttagtttgt ttgctattac
    10501 agcaatcgtt ctaagtgcat ttttctatgt tatgacgatt caaaaaatat cacaaattgg
    10561 cattttgaaa gcaattggta ttaagacaag acatttattg agtgcgttag ttttacaaat
    10621 tttaacacta acaataattg gggtaggtat tgctgtgatc atcatagtag gactatcatt
    10681 tatgatgccg gtaacgatgc ctttttactt aacaacgcaa aatattttat taatggtggg
    10741 gatatttata ttagtagcga ttttaggtgc ctcactatca tttatcaaat tatttaaagt
    10801 ggatcctatc gaagcaattg gaggtgcaga ataatggcat tagtcgttga agatatcgtc
    10861 aaaaatttcg gagaaggttt gtctgaaaca aaagttttaa aaggtattaa ttttgaagtg
    10921 gaacaagggg aatttgtcat tttaaatggt gcctctggtt ctgggaaaac aacattgcta
    10981 acgatattag gcggattgtt aagtcaaacg agtggtacag tgctttacaa tgatgcgcca
    11041 ttgtttgata aacagcatcg tcctagtgat ttacgattgg aagatattgg ttttattttt
    11101 caatcttcac atttagttcc ttatttaaaa gtgatagagc aattgacact cgtaggtcaa
    11161 gaagcgggaa tgaccaaaca acaaagttca acaagagcaa tacaactttt gaaaaatatt
    11221 ggtttagaag atcgcttgaa tgtatatccg catcagttat ctggcggtga aaagcaacgt
    11281 gttgcgatta tgagagcatt tatgaataat ccgaaaatca ttttagcaga tgagcccaca
    11341 gcaagtttag atgccgatag agcaacaaaa gttgttgaga tgatacgtca acaaattaaa
    11401 gaacaacaaa tgattggtat tatgattaca cacgatcgaa gattatttga atatgcagat
    11461 cgagtgattg aattagaaga tggcaaaata actgattagt ggcttgtaaa gacgctaaat
    11521 gttaatgatt taagacatag tagtataaaa gttagataac agaatacgat ttgggtttac
    11581 aaaaaa
//
LOCUS       DANRDI010000084        11486 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.084, whole genome shotgun sequence.
ACCESSION   DANRDI010000084 DANRDI010000000
VERSION     DANRDI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.084"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(105..1979)
                     /locus_tag="PZ377_001706"
     CDS             complement(105..1979)
                     /locus_tag="PZ377_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDM6358577.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(2219..2677)
                     /locus_tag="PZ377_001707"
     CDS             complement(2219..2677)
                     /locus_tag="PZ377_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358578.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            2935..3462
                     /gene="isaB"
                     /locus_tag="PZ377_001708"
     CDS             2935..3462
                     /gene="isaB"
                     /locus_tag="PZ377_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDM6358579.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            3886..5415
                     /gene="aur"
                     /locus_tag="PZ377_001709"
     CDS             3886..5415
                     /gene="aur"
                     /locus_tag="PZ377_001709"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDM6358580.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            5782..6231
                     /locus_tag="PZ377_001710"
     CDS             5782..6231
                     /locus_tag="PZ377_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDM6358581.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            6234..6401
                     /locus_tag="PZ377_001711"
     CDS             6234..6401
                     /locus_tag="PZ377_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358582.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGVFE"
     gene            6578..7813
                     /gene="arcA"
                     /locus_tag="PZ377_001712"
     CDS             6578..7813
                     /gene="arcA"
                     /locus_tag="PZ377_001712"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDM6358583.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            7846..8856
                     /gene="argF"
                     /locus_tag="PZ377_001713"
     CDS             7846..8856
                     /gene="argF"
                     /locus_tag="PZ377_001713"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDM6358584.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            8947..10377
                     /gene="arcD"
                     /locus_tag="PZ377_001714"
     CDS             8947..10377
                     /gene="arcD"
                     /locus_tag="PZ377_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDM6358585.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            10394..11335
                     /gene="arcC"
                     /locus_tag="PZ377_001715"
     CDS             10394..11335
                     /gene="arcC"
                     /locus_tag="PZ377_001715"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDM6358586.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
BASE COUNT         4084 a         1552 c         2161 g         3689 t
ORIGIN      
        1 ggcatgaggt aatagctaca attttcataa aatcatctcc ttttctatat tgtaagcgta
       61 ttctcgatac taaaaaaaag aataattacc gttactagtg gcaattattc ttgtaagtat
      121 tcaaataact gttgctttaa actatgatca tctaaactac ataaatggtt cactgaatca
      181 tcatccaagt tagcaattaa ttgcatcatt tgttttgtaa aagctttgtc tttatgcgaa
      241 atcgctaaga aaaagacaag tttgacatcg tgttgtcgcc aaggaaaaac atcttttgtg
      301 cgaaaaataa gcacatgtga ttgtaaaact ttttcaggat ctccatgagg aatcgccata
      361 aaattaccta tgtatgtaga agatgatttc tcacgctcta aagctgattc gatatatcct
      421 tctacaatcg catgatgtgc ttgtaatatt ttttgagctt cttcaaaaat ttgcacagta
      481 tgccgtgatt tttgttcagt atttacgaca aggaaattga cagtgtccat atgatgatgt
      541 gcttgaaccg gattttgctt ttgcttcaca acgtgtctga ttttgtgacg atcatcttca
      601 gaaaataatg gtgcaacctt gatagtcgtc aggtgcttag gaagtatgtt tagcgtttgt
      661 ttaggaatat catgggtcgt tattaataaa tctacattgt caaagtgata gtgtgttata
      721 ttttctagtt taatcgtatt tatcactgac aactcttcgg ataagttatt tattttagtt
      781 tctaaaaaat tcgacacacc tagaccataa taacaagcaa tgactacatt taattgtgtt
      841 ttggtacgac gctcgatggc agcttgaaaa tgaattgtta aaaatgcaat ttcatcttcg
      901 ctcatctcta tatcagtatc aattgctaat ttatcaatcg cttcaaaaag tgtgttaaac
      961 acaaagggat agagtttttt aatctctata actaaaggat tgtttaaata aatgttttga
     1021 gtgatacgta aatatgcttt actaaaatga ttatataaat tttgttgtaa aatcgaatct
     1081 tcattgaaag gtacatgaat acgttgctgc atcaattcga ttaagcgatc aatataactt
     1141 tgtataaata tacgttctat gccaatatcg agtttattaa aatgataagc aataaagaat
     1201 gaaaacatat tgattacttt ttcgttcaag tcataaccta atctttcgtt gatttgctta
     1261 atgcaagatt gagatatcaa ttttgaatcg tggtttaacg tgtctagctc acttaaagat
     1321 tgcttacgtt taatgattaa aatgagatga attaaaagct gttgtatttg aatatcagtt
     1381 gtttcaatac tatgttgttg aagtgtctct tgtataatat gcgaaatcat cctttggtgt
     1441 gaatcaggta attcatttaa aattaggtct tcaacatgta catgccctga tgataattga
     1501 tttaaatgga tgatggcatt agtgatatca ttatctgttc catcgaccac aataccttgt
     1561 tttttcttga ttgttaaagc gatattaaat ttttgacacc attgttgaat tcgaacgaaa
     1621 tagtcgttga gcacagtttt agataataga taatcttgct ctagttgttg caatgtctgc
     1681 gatttattgt gcattagcag ttgatacgcc atagataata atagtttttc tttatatgat
     1741 tgaatatgcg tgtaacgttc agttatcgtt tctaaggtat actgagatgt atttaattga
     1801 tatcctttag atttaatact tacgatgata tcatccataa aattgctatt gataacatgg
     1861 atatcattgc gaactgtgcg gtttgaaaca ttgacatgtt tagcaatttc attagaacta
     1921 atgtgctttg atggattttt aataaaaaac tggagtagtt ttaagtgtct atcaagcatt
     1981 taacatgtac ctcctttcta aatttttcgt gtaagcgttt ttaagggtgt attaatatta
     2041 ttaaacatga gagcttatac atacgtcaat gacattaaag cgaactttta tatgatttta
     2101 acagagtgcg aattatgcaa ataaagaaca gcagtaagat atttcaaata gaaaaatatc
     2161 tcactgctgt ttttctgaat ttatgcatct ggtacttgtg gacgtatcag gcaaatgatt
     2221 aatttttagg tgattgtgct tgaggtgttt gtttagaagg cgtattggta ttatttgatt
     2281 tattagcagg tggtgtgttc tgttgttgat tattttggtt gtttgttgac gattgactgc
     2341 caccaccgtg agtattattt ttatttgaat ttgagtcttt tggctgagct ggttgcgtat
     2401 tcgatggtac tgttgaatgg ttgttaggaa catttgacgg tgaagatggt tgattgtcat
     2461 tagtaacgtt attgccatta ttttgatttt tatctgtttt gtctgcacta tcatcttgtt
     2521 gatcattttc ttcggtttct gtctttttat gcgtagattt attttcttgt tcttggttat
     2581 tacttgatga tgtagaatga tttattgcag cataaattgc gaacccaagt gctccgatta
     2641 atacgataat tacgatggta ataagaagtt ttttcaaata taacgcctcc atgttaatga
     2701 aaagtagttg atacgtgaag ctaaataatt tatttcaata ttgccataat cattttaaaa
     2761 aataaatatt agaaaagtag gcgatattaa tattttcatt gttttttgtg tacttcaatt
     2821 agtataacaa aactgttatg tatttataaa tataatcatt agtattttta tggctgaaaa
     2881 agttataata aaagtgtaag gaataaaata tttgtatgga aaagagagat aattatgaat
     2941 aaaaccagta aagtttgtgt agcagcaaca ttagcattgg gcacactgat tggagtaaca
     3001 gttgttgaaa attcggcgcc aacaagtaaa caggcacagg cagcaataac cccatattat
     3061 acttataatg gttatattgg taataatgct aattttattc tggataagaa ttttattaac
     3121 gcgattaagt atgataatgt gaaattcaat ggtattaaat tagctaaaac gaatacgata
     3181 aaaaaagtag agaaatatga tcaaactttt aaaggtgttt ctgcaaaagg aaacgaagca
     3241 agtcaattgc aatttgtagt taaaaataat atttcattaa aagatatcca aaaagcttat
     3301 ggcaaggact tgaaaaaaga aaatggtaaa acaaaggaag ctgatagcgg tattttttac
     3361 tatcaaaatg ctaaaaagac attaggcatc tggtttgtcg ttgatcataa tagagttgtc
     3421 gaagtaacag ttggacatac accatacaaa acaagtaaat aaaataatgg catattaagg
     3481 ctagagtgtg aggagtgata ccgcattcta gtctttttta ttaaataata acgattattg
     3541 cgtcttacat agttgtttga aattgataaa aatgtttatt tgattatgat tatttaaata
     3601 gttatctcac atatttcaag cattgattaa gtatatagag cactattttg tatttgttaa
     3661 tattttcaca aaaataaagc cttgataaat tttaaaatat aattaagctc aatttttaaa
     3721 attttattta gctacagata acatttttaa aaaagaaaag aatcaataaa taaaatcaac
     3781 gaacaaaaag tatagaaata aatagaaata atcatttact tttatgaaaa attaaattaa
     3841 tattttattt ataagatgtt tttaagattt caggaggaat gaaatgtgag gaaattttca
     3901 agatatgcat ttacaagtat ggcagcatta accttgttga gcactttatc accagcagca
     3961 ttagcgattg attcaaaaaa taaaccagct aattctgata ttaaatttga ggtgactcaa
     4021 aagagtgatg cggtcaaagc attaaaagaa ttgcctaaat ccgaaaatgt aaaaaatatt
     4081 tatcaagatt acgctgttac tgatgtaaaa actgataaaa aaggatttac gcattataca
     4141 ttgcaaccga gtgttgatgg tgttcatgca cctgacaaag aagtgaaagt acacgcagac
     4201 aaatcaggaa aagtcgtttt aatcaatggg gatactgatg cgaagaaagt aaagccaacg
     4261 aataaagtga cattaagtaa agatgacgca gccgacaaag catttaaagc agttaagatt
     4321 gataagaata aagcgaaaaa tcttaaagat aaagtcatta aagaaaacaa agttgaaatc
     4381 gatggtgaca gtaataaata cgtttataat gttgagttaa ttacagtgac accagaaatt
     4441 tcacattgga aagttaaaat tgatgctcaa actggcgaaa ttttagaaaa aatgaactta
     4501 gttaaagaag ctgcagaaac tggtaaagga aaaggtgtac ttggcgatac aaaagatatc
     4561 aatatcaata gtattgacgg tggatttagc ctagaagatt taacgcatca aggtaaatta
     4621 tcagcattta gctttaatga tcaaacaggt caagcaacat tgattactaa tgaagatgaa
     4681 aacttcgtaa aagatgagca acgtgctggc gtagatgcaa attattacgc taaacaaaca
     4741 tatgattatt acaaagacac atttggtcgt gaatcatatg acaaccaagg tagtccaatt
     4801 gtttcattaa cgcatgttaa taactacggt ggtcaagata acagaaataa tgccgcatgg
     4861 atcggtgaca aaatgatcta tggtgatggt gatggtcgca cattcacaag tttatcgggt
     4921 gcaaatgacg tagtagcaca cgaattaaca cacggtgtga cacaagagac agcgaactta
     4981 gaatataagg accagtcagg cgctctaaat gaaagctttt cagatgtttt tggatacttt
     5041 gtagatgacg aggatttctt aatgggtgaa gatgtctaca cacctggaaa agagggagac
     5101 gctttacgca gcatgtcaaa cccagaacaa tttggtcaac cagctcatat gaaagactat
     5161 gtattcactg aaaaagataa tggtggcgta catacgaatt ctggaattcc aaataaagca
     5221 gcttataacg tgattcaagc aatagggaaa tctaaatcag aacaaattta ctaccgagca
     5281 ttaacggaat acttaacaag taattcaaac ttcaaagatt gtaaagatgc attataccaa
     5341 gcggctaaag atttatatga cgagcaaaca gctgaacagg tgtatgaagc atggaatgaa
     5401 gtaggcgtgg agtaaaaata tataaacaag aagaagtaat gttaaacact tataaataat
     5461 taaattttaa atacagctta tatatggggt atacctacat tgataattga ctttgtaggt
     5521 ataccctctc tttgtaatta gatgagagga gtgtgagggt tgtctgccga aagactactc
     5581 ggcagtctaa aatcattaca agtagtagat atgtgataat taaatgctga cttagaatac
     5641 aaaattcatt ttaaaagttg tcacaaaaaa tttacatgta tttttattat attttgcaaa
     5701 acaaagtgtt aaattataaa tgaaacatgc atgaatttat tttttaatac aagaaacgta
     5761 actaccaaag gagtttacaa tatgaagaaa agtaaacgat tagaaattgt ttctacaata
     5821 gttaaaaagc ataagattta taaaaaagaa caaatcattt catatattga agaatatttt
     5881 ggtgtaagat atagtgcaac aacaattgct aaagacttga aggaactaaa tatatatcgt
     5941 gtccctatcg attgtgagac atggatttat aaagctatta ataatcaaac agaacaagag
     6001 atgagagaaa agtttagaca ctattgtgaa catgaagttc taagttcaat catcaatggt
     6061 tcatacatta tcgtcaaaac ctcacctggt ttcgcccaag gcataaacta ttttatcgat
     6121 cagctaaata tagaagagat attaggtacg gtgagtggaa atgacactac attaatctta
     6181 actgcctcaa atgatatggc agaatacgta tatgcaaaat tatttaaata gacatgtatc
     6241 aaatgaataa taaaaatttg tttcgtatca cgtgtactca agttagttac caaatattaa
     6301 cttgtgtacg cgttttttta tggaaagaaa gaattcatag tcattcaatt gactgtataa
     6361 aaaactttat acaacatgtt tttatgggtg tttttgaata aaaaatgtat attttgaccc
     6421 aaaatgcctt tatttatgta taaaaatcca ttattatgta ttgtataaca aaaagatatg
     6481 aaattttcga ctttctttat gtgaatataa tcacatgtaa gcgtttgaag attgtctata
     6541 ctctaaatga attcaaagat aaaaggagga aatagacatg acagatggtc caattaaagt
     6601 aaatagcgaa attggagctt taaaaactgt gttacttaag cgtcctggaa aagaattaga
     6661 aaatttagta cctgattatt tagatggatt actatttgat gatattccat atttagaagt
     6721 agctcaaaaa gagcatgacc attttgcgca ggtgctaaga gaagagggtg ttgaagtact
     6781 ttaccttgag aagttagcag ctgaaagtat tgaaaatcct caagtaagaa gtgaatttat
     6841 tgatgatgta ttagcagagt ctaaaaaaac aatattaggt catgaagaag aaattaaggc
     6901 attatttgcg acactttcta atcaagaact tgtagataaa ataatgtcag gggtacgtaa
     6961 ggaagaaatt aatccgaaat gtacacatct agtagagtat atggatgata agtatccatt
     7021 ctatttagat ccaatgccaa acctttattt tactagagat ccacaagcct caataggaca
     7081 cggtataaca atcaatcgga tgttctggag agcacgacga cgagaatcaa tatttattca
     7141 atatattgta aagcatcatc ctagatttaa agatgcgaat attccaatct ggttagatcg
     7201 agattgccca ttcaatattg aaggcggcga tgaacttgtt ttatctaaag atgtcttggc
     7261 tataggcgtt tcagaacgta catctgcaca agctattgaa aagttagcgc gacgtatttt
     7321 tgaaaatccg caggcgacgt ttaaaaaagt agtagcaatt gaaattccaa ctagtcgaac
     7381 ttttatgcac ttagatacag tatttacaat gatagattat gacaaattta caatgcattc
     7441 agccatttta aaggcagaag gcaatatgaa tatatttatt attgaatatg atgacgtaaa
     7501 taaagatatt gccatcaaac aatctagtca tttaaaagat actttagaag acgtactagg
     7561 tatagatgat atccaattca ttccaacagg aaatggtgat gtcattgatg gtgctagaga
     7621 gcaatggaat gatggctcaa atacattatg tataagacca ggcgttgtag tgacttacga
     7681 tagaaactat gtatcgaatg atttattgag acaaaaaggc attaaagtca ttgaaatatc
     7741 tggtagcgag ttggtacgtg gacgtggggg ccctagatgt atgagtcaac cattattcag
     7801 agaagacatt taacgtaata aattatagaa acgagggtga aaataatgac agaaattcaa
     7861 aaaccgtatg atttaaaagg cagatcatta ttaaaagaaa gtgattttac caaagcagaa
     7921 ttcgaaggac ttattgattt tgcaattaca ttaaaagagt ataagaaaaa cggtattaag
     7981 catcactact tatctggaaa aaatattgca ctactattcg aaaagaattc gacgagaacg
     8041 cgtgctgcgt ttacagttgc gtctattgat ttaggtgcgc atccagaatt tttaggaaaa
     8101 aatgatattc aattaggcaa aaaagaatct gtagaggata ctgcgaaagt attaggtaga
     8161 atgttcgatg gtattgaatt ccgtggtttt tcacaacaag ctgttgaaga tttagcgaag
     8221 ttctctggtg taccggtgtg gaatggatta acagacgatt ggcatcctac acaaatgtta
     8281 gctgatttta tgacaataaa agagaatttt ggatatctag aaggaataaa cttaacttac
     8341 gttggagatg gacgtaataa tattgcgcat tcattaatgg tagcaggtgc tatgttaggt
     8401 gttaatgtaa gaatttgtac acctaaatca ttaaatccaa aagaggcata tgttgatatt
     8461 gcaaaagaaa aagcgagtca atatggtggt tcagtcatga ttacggataa tattgcagaa
     8521 gcagttgaaa atacagatgc tatatataca gatgtttggg tatcgatggg tgaagaaagt
     8581 gaatttgaac aacgtattaa tttattaaaa gactatcaag tgaatcaaca gatgtttgat
     8641 ttaacaggta aagattcaac gatattctta cattgtttac cagcattcca tgatacaaat
     8701 acactttatg gacaagaaat ttatgaaaaa tatggattag ctgaaatgga agttacagac
     8761 caaatcttta gaagtgaaca ttcaaaagtg tttgatcaag ctgaaaatag aatgcataca
     8821 attaaggcag taatggcagc aacattgggg agttaatcac taaatggaac gatatgaata
     8881 tgatgtgtct gatgatataa gtgtcatgta cagacacctc atattggtat taaaggagaa
     8941 atgaatatga acgaatcagg agataacaaa ctcagtaaat cttctttaat tggactagtt
     9001 ataggatcca tgattggtgg cggtgcgttc aatataatgt ctgatatggg cggtaaagcc
     9061 ggtggattag ccattattat tggttggatt attacagcta taggaatgat ttcattagcg
     9121 ttcgtatttc aaaatttaac caatgaacgg ccggagctag acggtggtat ttatagttat
     9181 gctcaagcag gatttggcga ttttgtagga tttatcagtg cttggggata ttggttctca
     9241 gcgtttttag gcaatgttgc ctatgcaaca ctattgatgt cagcagtagg taactttttc
     9301 ccgattttta aaggaggcaa cacattacca agtgttattg tcgcctcgtt actactctgg
     9361 ggtgtccatt tcttgatttt aaaaggcgtt gaaacagcag catttatcaa tagtattgtt
     9421 actgttgcaa agttaatacc gattttactt gtaatcatat gcatgataat tgcattcaat
     9481 tttgacactt ttaaaacagg ctttttcagt atgacgtcag agggtgtatt gccatttagt
     9541 tgggcgagca caatgagcca agttaaaagt acgatgctag tgacagtttg ggtgtttatc
     9601 ggtatcgaag gtgcagtaat tttttctagt agagctaaaa ataaaaaaga tgtaggtagt
     9661 gccacggtta taggacttat atcagtttta attatctatt tcttattaac tgtattagct
     9721 caaggcgtga ttttgcaaaa tcatatttcg caattagatt cgccaagtat ggcacaggtg
     9781 cttgcaacta ttgtaggtgg ttggggatct acacttgtaa atattggttt aattatttcg
     9841 gtactaggtg catggttagg atggacactg cttgctggtg aattaccttt cattgttgca
     9901 aaagatggat tatttccaaa atggtttgct aaagaaaata aaaatggagc acctgtaaat
     9961 gcactgctta ttaccaatat attagtacaa ttatttttaa taagtatgct atttacacag
    10021 agtgcgtatc aatttgcatt ttcactagca tcaagtgcta ttttataccc ttacatgttc
    10081 agtgcatttt accaagttaa atacacttta gagcatcgac agcaagcaac tactaaacaa
    10141 tggacgattg gtatcatagc ctcaatttat gctatatggc ttatatatgc agcaggtatc
    10201 aattacttat tattgacgat gttactttat attccagctc ttcttgttta tacaatcgtt
    10261 caaaagaata atcagacacg tttgattaaa tcagactata ttctttttat gattattatc
    10321 gtacttgcag ttatcgggtt aattaagtta ttgatgggaa cgataaatgt tttttaaaag
    10381 gagcgacaaa aatatgaaag agaaaattgt cattgcatta ggcggtaatg cgatacagac
    10441 aacagaagca acagctgaag cacaacaaac agctattaga tgtgcgatgc aaaaccttaa
    10501 acctttattt gattcaccag cgcgtattgt catttcacat ggtaatggtc cacaaattgg
    10561 aagtttatta atccaacaag ctaaatcgaa cagtgacaca acgccggcaa tgccattgga
    10621 tacttgtggt gcaatgtcac agggtatgat aggctattgg ttggaaactg aaatcaatcg
    10681 cattttaact gaaatgaata gtgatagaac tgtaggcaca atcgttacac gtgtggaagt
    10741 agataaagat gatccacgat ttgataaccc aactaaacca attggtcctt tttatacgaa
    10801 agaagaagtt gaagaattac aaaaagaaca gccagactca gtctttaaag aagatgcagg
    10861 acgtggttat agaaaagtag ttgcgtcacc actacctcaa tctatactag aacaccagtt
    10921 aattcgaact ttagcagacg gtaaaaatat tgtcattgca tgcggtggtg gcggtattcc
    10981 agttataaaa aaagaaaata cctatgaagg tgttgaagcg gttatagata aagattttgc
    11041 tagtgagaaa ttagcaacgc tgattgaagc agatacctta atgattctta cgaatgtaga
    11101 aaatgtattt attaacttta atgaacctaa tcaacaacaa atcgatgata ttgatgtagc
    11161 aacactgaaa aaatacgcgg cacaaggtaa gtttgtggaa ggatcgatgt tgccaaaaat
    11221 agaagctgcg atacgatttg ttgaaagtgg ggaaaacaaa aaagttatca ttaccaattt
    11281 agagcaggca tacgaagctt tgattggtaa taaaggtaca cacattcaca tgtagcactt
    11341 tatcacgcga caaaacatta aatatgtttc tccgttgatt caaatgaaaa agttgtctgc
    11401 tgacactttg caaggtttga aggagtttaa cttatgacag aaaactttat tttgggtaga
    11461 aataataaat tagaacatga actaaa
//
LOCUS       DANRDI010000085        11483 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.085, whole genome shotgun sequence.
ACCESSION   DANRDI010000085 DANRDI010000000
VERSION     DANRDI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.085"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(48..1304)
                     /locus_tag="PZ377_001716"
     CDS             complement(48..1304)
                     /locus_tag="PZ377_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HDM6358587.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMPLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(1626..2105)
                     /gene="rlmH"
                     /locus_tag="PZ377_001717"
     CDS             complement(1626..2105)
                     /gene="rlmH"
                     /locus_tag="PZ377_001717"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDM6358588.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(2473..4791)
                     /gene="adsA"
                     /locus_tag="PZ377_001718"
     CDS             complement(2473..4791)
                     /gene="adsA"
                     /locus_tag="PZ377_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDM6358589.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(5019..5819)
                     /locus_tag="PZ377_001719"
     CDS             complement(5019..5819)
                     /locus_tag="PZ377_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDM6358590.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(6209..6997)
                     /locus_tag="PZ377_001720"
     CDS             complement(6209..6997)
                     /locus_tag="PZ377_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDM6358591.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(6998..8332)
                     /locus_tag="PZ377_001721"
     CDS             complement(6998..8332)
                     /locus_tag="PZ377_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358592.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8325..10151)
                     /gene="walK"
                     /locus_tag="PZ377_001722"
     CDS             complement(8325..10151)
                     /gene="walK"
                     /locus_tag="PZ377_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDM6358593.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(10164..10865)
                     /locus_tag="PZ377_001723"
     CDS             complement(10164..10865)
                     /locus_tag="PZ377_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6358594.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         3249 a         2232 c         1701 g         4301 t
ORIGIN      
        1 tctattttta ttagatcaca attttatttt agctcttgtt caagtaatca tttttcgcca
       61 aaaactttat actgaatagc ttctacatta aatactttgt caatgagatc atctacatct
      121 ttaaattcag aataatttgc atatggatct ataaaataaa attgtggttc tttaccggaa
      181 acattaaata ttcttaatat taaatatttc tgcttatatt ctttcatagc aaacatttca
      241 tttagcggca taaaaaatgg ttcctcaata ctagaagatg tagatgtttt aatttcaata
      301 aatttttcta cagctttatc tgtatttgtt ggatcaaaag ctactaaatc atagccatga
      361 ccgtgttgag agcctggatt atcatttaaa atattcctaa actgttcttt cttatcttcg
      421 tctattttat tatcaattag ctcattaaag taatttagcg ctaatttttc tccaacttta
      481 ccggttaatt tattctcttt atttgatttt tcaatttctg aatcattttt agtagtcttt
      541 gatacacctt ttttatattt tggaattatt cctttaggtg cttccacttc cttgagtgtc
      601 ttatcttttt gtgctgttct aatttcttca atttcgctgt cttcctgtat ttcgtctatg
      661 ctattgacca agctatcata ggatgttttt gtaacttttg aagctaattc attaaatagt
      721 tctaaaaatt tctttaaatc ctctagcata tcttcttctg tgaatccttc attcaaatca
      781 taatatttga atcttattga tccatgagaa tatcctgatg gataatcatt ttttaaatca
      841 taagatgaat ctttattttc tgcgtaataa aatcttccag tattaaattc atttgatgta
      901 atatatttat tgagttcgga agataaagtt aatgctcttt gttttgcagc atttttatcc
      961 cgcggaaaca tatcacttat ctttgaccat ccttgattca aagataagta tatgccttct
     1021 ccttccggat gaaaaagata taccaaataa tatccatcct ttgtttcttt tgttatattc
     1081 tcatcatata ttgaaatcca aggaacttta ctatagttcc cagtagcaac cttccctaca
     1141 actgaatatt tatcttcttt tatatgcact tttaactgct tgggtaactt atcatggact
     1201 aaagttttat atagatcacc tttatcccaa tcagattttt taactacatt attggtacgt
     1261 ttctctttaa ttaatttaag gacctgcata aagttgtcta tcatttgaaa ttccctccta
     1321 ttataaaata tattatgtct cattttcttc aatatgtact tatttatatt ttaccgtaat
     1381 ttactatatt tagttgcaga aagaattttc tcaaagctag aactttgctt cactataagt
     1441 attcagtata aagaatattt cgctattatt tacttgaaat gaaagactgc ggaggctaac
     1501 tatgtcaaaa atcatgaacc tcattactta tgataagctt cttaaaaaca taacagcaat
     1561 tcacataaac ctcatatgtt ctgatacatt caaaatccct ttatgaagcg gctgaaaaaa
     1621 ccgcatcatt tatgatatgc ttctcctcgc ataatcttaa atgctctgta cacttgttca
     1681 attaacacaa cccgcatcat ttgatgtggg aatgtcattt tgctgaatga tagtgcgtag
     1741 ttactgcgtt gtaagacgtc cttgtgcagg ccgtttgatc cgccaatgac gaaaacaaag
     1801 tcgctttgcc cttgggtcat gcgttggttc aattcttggg ccaatccttc ggaagatagc
     1861 atctttcctt gtatttctaa tgtaatgact gtggattgtg gtttgatttt ggctagtatt
     1921 cgttggcctt ctttttcttt tacttgctca atttctttgt cactcatatt ttctggtgct
     1981 ttttcgtctg gaacttctat gatgtctatc ttggtgtatg ggcctaaacg tttttcatat
     2041 tctgctatgg cttgcttcca atatttctct tttagtttcc ctacagctaa aatggtgatt
     2101 ttcatgtcgt ttggtcctcc aaattgttat caactttcca gttatccaca agttattaac
     2161 ttgttcacac tgttccctct tattatacca atattttttg cagtttttga tattttcctg
     2221 acatttatgc ccgattttta gtgtataacc tgtgtatttg tggataactc tgtgggcatt
     2281 gtgggtattt ctctttaaat tgtacacaat tctggataac tatccccatt tctgtggata
     2341 aacaccttga tgtcttatta cttatcctcg tttttatatc ccatatttat caacggttat
     2401 cgcttttttt cataaaaaaa cactaccgta cctcttattt aagaaataca gtagtattat
     2461 tttcaatata ttttagctag cttttctacg tcgtacagtc gcgattaaac ctatacctgc
     2521 taataataca aacatcgctt ctgggcttga actttgatta gttccagttt ttggtaactg
     2581 tttctcatgc gcgctacctt taggcactga cattctagcc aattgtttcc cactcttgct
     2641 tgatacagta gctccttcta ttgtgcgacc agaaccttct gtaccgctac taacagttcc
     2701 tctatgcgct agcaacaata caactttacc tggagctggt tttttcgctg gatccatcac
     2761 tttgtcgtca ccaattggtt gtgtatcttt accagactta ctacctttgc taccttgttg
     2821 tcctttagct ggttgttcac ttactgctgg tttacctaat aacatacgtt gtggttctgt
     2881 cgtatcatac ttagctaagt tagctgtttt taaataactt gctagtactt gatctaatga
     2941 aataccttct tctctaggac caccgaacat actatatccg tcgccacctg atgctgtgaa
     3001 gtcattcatc gttacgtgat atacacgttt taaatcaata ttttcaaact tacctgtctc
     3061 tttatttaaa atttgaatag cattaattcg tttgccagac ggtttattta tatcatagta
     3121 aacacggatt gaatcagaga tatgtagtaa accgccattc gctgttaaca ctgtcttacc
     3181 gtccttttgt gttgttggtg cgcctaaact atgttcgaaa gccgtccaga cgtctgaacc
     3241 ttttacatca atttgcgcaa tcgtatttcc aaatggtaat actgagatta aatcatagcg
     3301 tgtcacctta ccttttgcga tagaggcacg aatacctcca ccatttgtca cggcaaagtc
     3361 agtcttttta gagaaattct taacgccata cgcttccata gcatctgcaa tcgcgtttcc
     3421 taaatttgtt tcacgcgttc taacgtcatc tctttctcct ttgaaatcaa tggtattgtt
     3481 tggaataatt acctctgcag tttgtgctct aaatgtttga tcagcttgat taatttgttc
     3541 agctaatgct ttgttcggtg ttacattttc aacgtcttta acattaatca atgacggttt
     3601 aatattcgat acctctccat tgcgataatt aaatgtaatc ttaccgatat tcgcaagtgc
     3661 tgtacctgtt tgtgccaatg catcattgtt ataaatttga ccattttgaa gtactgtatg
     3721 tgaatgacca tcaataactg taatacgttt cttcaattgt ggattttgac ttaattgttt
     3781 cactaagtaa tcaccacgcc atgtttcttg tgttgaagga tcaattccta aatgtgatat
     3841 aacaacaaat gtatctacgt ctttataaat acgcatcatt tccgctgtca cactttgtaa
     3901 tggatctcta aattcaacgc ctttaatgcc ttcaggtctt gttttcgtct ttgtttctgg
     3961 tgtcgttaca ccaataattc cataacgaat accatttttt gttacaatcg ttgaaggctt
     4021 aaacgcgcgt tttccatctt tataaacgtt agtacttagc atcgggaagt ctaacatacc
     4081 ctctaacttt ttcaactgat cgtatccaaa gtcaaattca tggttaccga ctgccatagc
     4141 atcataacct actgcattca ttgctttagc catttcttca cctttagact ggtttgaaag
     4201 tggtaaacct tggaaggcgt ctcctgcgtc taacattaaa tcaggctttt cttgttcttt
     4261 tactgttttt aatttagcca taccgatgac acgccctttt tcttcggcta gtcggccatg
     4321 gatatcattt gtatgtaata ttttatgtgt tgttgtttgt ggtgcattag cagcaaacat
     4381 tcgtggtgaa agtgatgctg ttttagcatt tgataatttg aacgttgctg tgtgagttgg
     4441 tttttgtgtt gaggcttgtt gtgtatctac gtcgcgtgtt tcgtttactt ttgttgaaac
     4501 tactgtagat ggtttgttag atggtgcgtt ggatgttact ttgctgtttg tatcatcagc
     4561 tgttccctgc gctgatgctg atacgttggt tgccgcttct ttgccagaat gatgagccgc
     4621 ttcctttgtt ggcggtactt gttgcttatc tgttgttgct ttgtctatcg ttgttactgc
     4681 atgtgctttc attggtgtat gctgctcagc cgcgttcgag acttgccata atcccattac
     4741 actaaaaagc aaaacgagcc atacacttgt tttaagtaat aaagctttca tttcgttccc
     4801 tccattaact gtcatacacc aatatattat cataatcctt atattaaaat gattgatttt
     4861 atgtaaatta tttataaatt acatttaata tcgaattatt ttacacattt caatcaagcc
     4921 gattcaactc aacaacagcc atttcccacc aacaacataa aaaagcaccc gataaagtcg
     4981 tctcacagca atgcggaact ttatcggatg actctcattt atattgtata tattggcgtt
     5041 ggaatagctt tatccgtatc acatagcaat acttcttttt ccgtatcaat atcgtgttcg
     5101 ttcaatactt ggccaacact catacgcgcc aaatctttca tgttattgtc ttgtgataaa
     5161 tgcgataggt aaatacgttt cgtgttacct gtaatcacgt ctgtcattgc atgagccgca
     5221 tcctcattag atacatgacc catatcgcct aaaatacgtt gtttcgtctt ccatggataa
     5281 cgacacattc tcaacatatc gacgtcatga ttactctcaa aaataaacgc atcgctgcca
     5341 cgtatcatac ctttcatacg atcagacacg taacccgtat ccgttaaaat cgtaaacttc
     5401 ttatagttat tatggaaaat ataaaattgc ggatctattg catcatgtga cacgttaaac
     5461 gattcaacat cgaaacctgc aatagatttt gtttcataag gattaaaaat gaatttctga
     5521 tccataggga tgcgactatc tttcttttca attgcctgcc aagtcttttc attcgcataa
     5581 attggcaatt gatatttacg cgccaaaaca cctaatcctt taatatgatc aatatgttca
     5641 tgggttacta aaataccatt taaatcttga atattacggt caatttgact aaacaattct
     5701 tccattttct ttccagtcaa accaacatca actaatagac tacctttttc attttctaca
     5761 aaagtggcgt tacctgtact accacttgct aatacactca tgcgtatcaa gcggctcatc
     5821 ctttctcttt aaacagtcaa tctctctata tgactgcaat atacatcagt gcaaatatac
     5881 gattttccct acagaaacta tcagtatatt agctgttttg tatacctttc ctattatgta
     5941 actttagttc atcgaccggt ttcttatatt taaattttga taatcacgtg caatgaattt
     6001 cttaattatt ataacaatga ttagacaaat actaaagttt ttaaaataac agttacacta
     6061 attttacaat gcggttctac ttaaaatatg aataaatgcg cacgttggag acgcaaaaac
     6121 atcagccata ataaaccgta ccagcaaaaa agctagcacc gcttctcaca tatgatgagc
     6181 ttgcaatgct agcttattac gattcatatt aatgattaat aattttaggg ttattatttg
     6241 tcgcttcgac atagtaagta ttcgttttat ccttaccgtc atgcttcact ttaatttccc
     6301 agtttggttg taacaattga acattcgttt cattcacgac tgagtagtaa ccgagtctag
     6361 cattaatgac ttcatcacct tttttcaagt aacgattata atataatgcc tcgattgctt
     6421 ttctcacacc aatcacttga tgcttcttat ctgcaccatc tgtgggctta atgtcatcca
     6481 tcattgattg tttataacta gtcgctttgt tatcttctat attaaagttc aacatcgctt
     6541 tactgttatt taaaatcgga aaatcatcat acgtttgttc atattttaca gaatctgaac
     6601 taatctcgct taattgatat tcagcacctt taaatacgcg cttgttaaca taatctttca
     6661 aatcttttaa gttattgtca ctgaccttta ccgattgact aatgttcgca ttcaataatt
     6721 ttccaccatc tgatgtggtc aaatccgaat cgcctttagc tttagaacta aagtcttttg
     6781 atcgccctgt aatttgctct aattttatac ctttaactga tttattcaat atactagtcg
     6841 gtactttaat ttcttcttgc tgaaaattaa cttcattgtt actctcgact tcattaatgt
     6901 gtgagcgatt gactttatta acataaatcg atactaacac gatgttgaca agaataaaca
     6961 cgaaaatgaa aagtgtcttt gtcagtttcc agttcattta ttcaagcctc ccatcgttat
     7021 aaacatacca ttcgccatca tattctacat accaacgcgg tacgagttca ctgttaatct
     7081 gcacttcaat gtgattatga tctgaattat cctgcatttc gtaaccgata gcgatgtttg
     7141 ttactttttc aaaattaata tcactattgt tcgctaagct tgaacgtaca gactctaatt
     7201 tcggcaacga tttattatcc tcactattta aaacaacgtc ggtgcgtaat aacgaacgac
     7261 gatagtcaaa gacgcctttt tcaccccaag tgacttgaat ttgattagaa ccttctttat
     7321 taaacgttgg ataaccatta aggaaacgtt gatatgttaa ctcgcctgac tgattattcg
     7381 tactaaacaa tctaaagtct tcgtttaaga aaccaccatg accattaata aaatcaaagg
     7441 ttcctggaat cgtttcttcc attttgctgg aactcgcttc atcttcggac aggtttttat
     7501 aatgatattt ttcattttta tcgttatagt ttgcgacacc tgtattattg ttgtaggttg
     7561 taacaccact ctttgaacta cgaacgatgg ttgaatcgtc aaatagtata gcattcattt
     7621 tctcaacact aatcgtgtta aataccatgc gatatgtttt taacttttca ggtttacttg
     7681 gtgcaaaaac atgcgtcgta cgatcaattg tatctttgtt tgtgatgata tctgtgtatg
     7741 gttgcatatc ttttttcact gctgctaatg catctaaaaa atgatcattt ttcgttgtag
     7801 ttgttaattt tacgtaatcg tggcgatctt tgcttatagc ataaagcacg atattatcat
     7861 cagcatcatg atctatgacc aaacgattga aattgaaatg atttggtact ttcgcattca
     7921 tgttcagtac ttgaccaaga tatgttgata acggtaaatc atacgtaaaa tcgaataata
     7981 taaaatcact gttcaaatca ggaatcatca agttatgatc acgacgaaca tgttccacgg
     8041 acttcacttc tttatttttc aaaggtttcg tcagtttatt cacattagat accgtcgcaa
     8101 tcgttccttc tggatgatca tttttcgaat gaataatctg aaatggcgta atagttgtat
     8161 ccattttggc tgtcataggt gtcgttaaag gtttcgtttc actcttctta ctatctgtat
     8221 tgtcgacatt tgcaatatca ggagaaaagt tccataccat atatgtcaat acgacactca
     8281 tcaagacgag tagtgctaaa atgacagatt taatatgctc cttattattc atcccaatca
     8341 ccgtcttcaa tgacttcaca tggaagtgtg ataaagatag atgtaccttg accttctaca
     8401 ctgtttgccc aaatacgacc attgtgcgct tccacaatct ctttcgaaat ggctagtcct
     8461 aatccagtac cacccatttt acgcgtacgt gccttatcta cacgatagaa tcggtcgaat
     8521 atcttatcga ctttattgat aggaatacca atgccattat ctttaatacg aatcgtcatt
     8581 cgattataaa gtggattttg tttcacgtgg aactcgacac gtttatcgcc tctagaatat
     8641 ttcatcgcat ttgtaatgac attatcaaat acttgcgtca ttttatcagg atcaaattct
     8701 gtgaaaatcg tctttttcgg aatatctcga ataaatgttg tatctttcgc agacatttca
     8761 tgtcgattaa taattttatt aatgaacatg ttaaagtcga taatttcttt gttgatttga
     8821 tcagactcat tatccatttt agataactgt agcaagtcat tgaccagtcg aatcattcgt
     8881 tctgtttctt cacgggtaac agataaaaat tgtggcgcaa gttcctcatc tttccatgca
     8941 ccttcttcaa gtgcttcaat gtaactattc atagaagtta aaggtgtacg taactcatgt
     9001 gatacattgg caacaaattc acgacgctca cgttcaactt gttgttgttc agttacgtca
     9061 tgtaacacag cgatataacc agttacaaat cctgtttcct gcacaatcgt actaaagtta
     9121 acacgtgcga ttagaccttc ttcttcattt aaatctaata agaaactatc attattctct
     9181 tgaatttctt ccagtttaaa ttcatcttca agacttaata cacttaacat gtaatatccg
     9241 atgatgtctt ctttcgccat accaagcatc ttgagtgcca tatcattgac gatacgaata
     9301 cgtccacggc ggtctgttgc aataatacca tcactcatat gggtgataac tgagtccagt
     9361 ctacgtttct cactttcagt attagcctgc gcttcttgta cacgtttaga caagttatta
     9421 aatgctaaag ctaattcgcc aatttcatca ttaccataaa tcttcacacg ttgcgtatag
     9481 ttacctctgg acatttcgac cgtctggtta cgcatatcgg tgattggttt ggtaatcgtt
     9541 cgcgctataa agaatcctag gatgactgtg attaataatg aaatagctgt accaacaatg
     9601 aatatttgat ttatattatt taattggtta taaacgtcat taatttttga ttcgatataa
     9661 atattaccaa ttaccttttt atcgacttta actgggatat tatataccca gacacggtcc
     9721 ttaccaccgc cataatcttt taaaattaaa tgatcgtttg attgtcctag tgatagtgct
     9781 ttttggacag aactatcatt cgctttttga ttgattagac tacggttaga ctgcttcgtc
     9841 gtcgcaataa taatttggtc tttatctata aaacgaattt ctccaatttc ttgacggttg
     9901 gcatactcac ttaataaatt ttgaatatct ttttgtgcat ttacggagcc cttttcgtca
     9961 tatacttttt caatactaat ttctaattgt ttcgcgtact gcgtaatatt cttcttaaaa
    10021 ttatcaagca gctctttttc aaggttattt gtaaaatata acccgataat ttgcatacca
    10081 atgataatca gtaatacata aacaattaca agtttagtat gaagggattg tagttgtttt
    10141 agccacttca ttcgtttcga cctctactca tgttgttgga ggaaatatcc aacgcctcta
    10201 cgcgtcacaa tatattcagg atgtgacgga tcatcttcaa tcttttcacg taaacgacga
    10261 atcgttacat cgaccgtacg tacatcgcca aagtaatcat agccccatac tgtttgtaat
    10321 aaatgttcac gtgtcattac ttgtcccata tgttttgata aataatggaa caattcaaat
    10381 tcacgatgtg ttaattcaat atcttcgcca cgttttttaa tagaatatgc gtctggataa
    10441 atcacaatat ctttaattgt gatttcattc gttacatttc cagtgtcttg tgctggttgt
    10501 gagtaatgac gacgtaagtt cgctttcaca cgtgcgatta attcacgcgt actaaacggt
    10561 ttcgttacat agtcatctgc acctagttct aaaccaagca ctttatcaat ttctgaatct
    10621 ttagcagtaa gcattattat tggcatttcg tattttttgc gcacttcacg acatacttcc
    10681 ataccatcac gaccaggtaa catgatatct agtaatacga tgtctggttc ttcttcataa
    10741 attaagtcga ctgcatcatt accatcgtat gcacagtaca catcgtatcc ttcttttttt
    10801 aagttaaatt ctaaaatatc agcaatcggt ttttcatcat caactacaac aacttttcta
    10861 gccatttgca taaacctctt ttcttaaatc attaatatta ttttatcatt ttttgttctt
    10921 tatcgtttat gtttgtgtaa aaaatcacag gatgtcgctt actatttttg ttattgtgcg
    10981 cattttccgt cttataacgt atagtaacct attatataat ttaccattta taagctatta
    11041 attctatttt ccaatataaa cttatatatt actatttttt attagtttct cacggcggat
    11101 tgtgacaatg atatttaaca caatgatgag gtctctattc caataataaa attaggataa
    11161 tggcaacttc tagttgcacg tcattgctca gaaaataaat atcattattc gttattcgtt
    11221 actttaacaa ttgatgttat ggaggactgg tggcatatca caacgaagaa atatgttgac
    11281 agtagcatat gtttgtacgt ctaataaaga ttttattgtc tacgtttatc tttcatatcc
    11341 aaccactaag ttacttgagg ttcatacatt ttaaagaaaa tggacgaaca gataaatcaa
    11401 tatgattatc attcgtccat atatctttta ttaatataat attaaacgca aaaaaatgcc
    11461 tatcctcttg gataggcatt tgt
//
LOCUS       DANRDI010000086        11310 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.086, whole genome shotgun sequence.
ACCESSION   DANRDI010000086 DANRDI010000000
VERSION     DANRDI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.086"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(122..1108)
                     /locus_tag="PZ377_001724"
     CDS             complement(122..1108)
                     /locus_tag="PZ377_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDM6358595.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            1636..2394
                     /gene="map"
                     /locus_tag="PZ377_001725"
     CDS             1636..2394
                     /gene="map"
                     /locus_tag="PZ377_001725"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDM6358596.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            2611..2997
                     /locus_tag="PZ377_001726"
     CDS             2611..2997
                     /locus_tag="PZ377_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358597.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            3012..3713
                     /locus_tag="PZ377_001727"
     CDS             3012..3713
                     /locus_tag="PZ377_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDM6358598.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            3710..4753
                     /locus_tag="PZ377_001728"
     CDS             3710..4753
                     /locus_tag="PZ377_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDM6358599.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            4743..5372
                     /gene="vraR"
                     /locus_tag="PZ377_001729"
     CDS             4743..5372
                     /gene="vraR"
                     /locus_tag="PZ377_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDM6358600.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(5488..6705)
                     /locus_tag="PZ377_001730"
     CDS             complement(5488..6705)
                     /locus_tag="PZ377_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDM6358601.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(6983..7258)
                     /locus_tag="PZ377_001731"
     CDS             complement(6983..7258)
                     /locus_tag="PZ377_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDM6358602.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(7265..7729)
                     /locus_tag="PZ377_001732"
     CDS             complement(7265..7729)
                     /locus_tag="PZ377_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDM6358603.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            7876..8076
                     /locus_tag="PZ377_001733"
     CDS             7876..8076
                     /locus_tag="PZ377_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDM6358604.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            8079..9335
                     /locus_tag="PZ377_001734"
     CDS             8079..9335
                     /locus_tag="PZ377_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDM6358605.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            9425..9955
                     /locus_tag="PZ377_001735"
     CDS             9425..9955
                     /locus_tag="PZ377_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDM6358606.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(10215..>11310)
                     /gene="yfkAB"
                     /locus_tag="PZ377_001736"
     CDS             complement(10215..>11310)
                     /gene="yfkAB"
                     /locus_tag="PZ377_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDM6358607.1"
                     /translation="YNDVKEHGQLTLSNIEFTTTNLCNMRCSHCAVGYTLQTVDPEPL
                     DMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPLLKYAHHRGIYTQMNSNLTL
                     PQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQPPLKAKLKLYEQMISNARTL
                     SEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEIHPMYPADFASQLNVLTLAE
                     MKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLSRLRNAKNVTTRNDPDGRSR
                     LNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLSSDLAKSLNCHCSEFSCLGP
                     NVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
BASE COUNT         3971 a         1700 c         1960 g         3679 t
ORIGIN      
        1 agagaatttc gaaaagaaat tctacaggca aagcgagttg gggtgggacg acgaaataaa
       61 ttttatgaaa atatcatttc tgtcccactc ccatggtgcc aattagcata aggtacttaa
      121 attaagcata tctgctgtct agcagtcgat aaatcattag aacttcgtat agtatatgac
      181 ttttaatttg attttcacca ctaatttcaa gtgcttttat agtcgaacgt aaagtttcta
      241 cagaatcatc ttctctctta aaagaaccat cataaaatat atctttgatg ctactactaa
      301 tttttagcaa tgccattttt tcgtcacctg aaaagttaac acgagtattt ttaggcaagt
      361 aaatgatatt tgataaatga gtgataaaca aacgattcgt atatgcacgt ttagttaatt
      421 gattgagtaa tttccaatca cattcttttt tcttatgata gcttaattca tcacgttgat
      481 aacttattaa cgtttcaact tgattattta aattgaaaat atttttatat gctttttcgc
      541 ttttatcaga ttgcagtctt gataagataa gttcttggca gcgattgtaa aataatttat
      601 acatcaaggc atctgtctta cttaattttt cttcgacctg accataatac ttaggtggaa
      661 acaccatgaa gttaattaaa cctgatgtca cgagtccaat aattgctgtc aatgttcgag
      721 acaaaaagtt gaatatgtag gcatcatgaa tacctggaat catagctaat gatgttagta
      781 cagcgacatt cgtaccaact tgcaatttga gttttgtaca gaataaaatc gtgaacgttg
      841 cactcaatgc atatgtaaaa ggtgattgat cgccgaataa atatgtaaat aatactgcaa
      901 agcctgcacc aattaccgta gcaggtaatc tacgataacc tttaataagt gatgccttgg
      961 cagttggttc aattgtgact acagctgtta aaatggcata gatgggtgtt aaatctagtg
     1021 ccatacaaaa gacagctgtt aaaaaaatgg caataccagt tttaattgtt ctggcaccaa
     1081 ttaaatgttt ataccattga tcgttcattt tttaacctct aatcatcgta aaatcttagc
     1141 gagcgcttta taataatagt atcgtacatt ggaaaagttc atgtatgtaa aatatttgaa
     1201 ataatcatac ataagcatta ctttgatttt catatacatt aatcaaagtt ctgggctcca
     1261 caatgttata ttgttagtag ttgactgaaa taaaatgcac ttgtaacaag catttttcaa
     1321 ttatagtccg gggccccaac atagagaatt tcaaaaaaga aattctacag gcaatgcagg
     1381 ttggcggggc cccaacacag aagctgacga aaagtcagct tacgataatg tgcaggttgg
     1441 cggggcccca acatagagaa attggatcta caatttctac aggcaatgca agttggggta
     1501 caacgataaa gaaatatttt ttctttatca cactatgtct cactcacttt ccaaaatact
     1561 aaagtaacat ctttagtata tcaaagaatt tttgctataa taagttataa ttatataaaa
     1621 aaggaacggg ataaaatgat tgtaaaaaca gaagaagaat tacaagcgtt aaaagaaatt
     1681 ggatacatat gcgctaaagt gcgcaataca atgcaagctg caaccaaacc aggtatcact
     1741 acgaaagagc ttgataatat tgcgaaagag ttatttgaag aatacggtgc tatttctgcg
     1801 ccaattcatg atgaaaattt tcctggtcaa acgtgtatta gtgtcaatga agaggtggca
     1861 catgggattc caagtaagcg tgtcattcgt gaaggagatt tagtaaatat tgatgtatcg
     1921 gctttgaaga atggctatta tgcagataca ggcatttcat ttgtcgttgg agaatcagat
     1981 gatccaatga aacaaaaagt atgtgacgta gcaacgatgg catttgagaa tgcaattgca
     2041 aaagtaaaac cgggtactaa gttaagtaac attggtaaag cggtgcataa tacagctaga
     2101 caaaatgatt tgaaagtcat taaaaactta acaggtcatg gtgttggttt atcattacat
     2161 gaagcaccag cacatgtact taattacttt gatccaaaag acaaaacatt attaactgaa
     2221 ggtatggtat tagctattga accgtttatc tcatcaaatg catcatttgt tacagaaggt
     2281 aaaaatgaat gggcttttga aacgagcgat aaaagttttg ttgctcaaat tgagcatacg
     2341 gttatcgtga ctaaggatgg tccgatttta acgacaaaga ttgaagaaga atagttcaac
     2401 atatactaag actaaagtat gaacatcatt tagttccgga gcctattcat attggtttcg
     2461 gaactgtttt ataataatta agaacacaat caatgcgtca tttcaaaaat atgttgtaac
     2521 aaagtagttt ttaagcaaac atatcatcga catcaacgaa gatacatagc gcatttggta
     2581 ttttaaaact tattataaaa ggtgatagtt atgaactatg ttgaacgtta tattgaacag
     2641 tttttgagag caacagtaag aaataatatc aagcactacc ttttaatgct agatgaaaaa
     2701 atgaaaaatt tagatgatta tatgcgttat ttaattacta aaaaagaaca acttagcaag
     2761 ttaattgaca gtctaatgct aacattagaa aataaatata ttgatattgc tgaagcattt
     2821 caaattcaat gtgcaagaga aatcaataat caagaaattg aaaatattaa atcagagttg
     2881 aataaagttg aagcatatta tgcacaaatt gaaactcaaa ttcaacaaac ttcaactgaa
     2941 aaaatagcaa cagaaaaaac atcgtatcta ataaattata tgaacgctgt ggcatagaaa
     3001 ggcggcgaaa catgacacac aaatatatat caacgcaaat gttgatcatt tttactgcat
     3061 taatgattat tgccaatttt tactacatat tttttgaaaa aattggcttt ttactcgttc
     3121 tattattggg atgtgtatta gtttatgtag gatatcttta ttttcataaa atacgtggcc
     3181 ttttggcgtt ttggataggc gcgctattaa ttgcattcac attattgtct aataagtata
     3241 caatcatcat cttgttcgtc tttttattat tacttattgt gcgttattta atacacaagt
     3301 ttaaaccaaa aaaagtagtt gcgacggatg aggttatgac ttcaccatct tttattaaac
     3361 aaaagtggtt tggtgagcaa cgtacaccag tttatgtata taagtgggaa gatgtacaaa
     3421 ttcaacatgg aattggcgac ctacatattg acttaacaaa agctgcaaat attaaggaaa
     3481 ataataccat tgttgttaga cacattttag gtaaagtgca ggttatattg ccggttaatt
     3541 acaatattaa tttacatgta gctgcttttt atggaagtac ttacgtgaat gaaaaatcat
     3601 ataaagttga aaataacaat attcatattg aagaaatgat gaaaccggat aactatacag
     3661 ttaatatcta cgtatcaacg tttatcggag acgtagaggt gatttatcga tgaaccacta
     3721 cattagaaca attggttcaa tgctcatctt agtatatagc atgctagctg catttctgtt
     3781 catcgataaa gtttttgtaa atatcatcta ttttcaaggt atgttttata cacaaatatt
     3841 cggaatacca gtttttttat ttttaaatct catcatcata ttattgtgta ttattgttgg
     3901 ttcggtactc gcttacaaaa tcaatcagca aaatgattgg attaagacgc aaattgagcg
     3961 ttcaatggaa ggcgaaacag ttggcattaa tgatcaaaat atagaaatat atagtgaaac
     4021 gttagattta taccatacac tcgtaccttt aaatcaagaa ttgcataagt tgcgacttaa
     4081 aactcaaaac ttaaccaatg aaaattataa tattaatgat gtgaaagtta aaaagattat
     4141 tgaagatgaa cgtcaaagac tagcacgaga acttcacgat tctgttagtc agcaactttt
     4201 tgcggcaagt atgatgctat ctgctatcaa agaaacgaag ttagaaccac cattagacca
     4261 acaaattcct attttagaga aaatggttca agattcgcag ttagaaatgc gtgctttgct
     4321 gttacattta agaccgcttg gtttaaaaga caaatcttta ggtgagggta ttaaagattt
     4381 agttattgat ttacaaaaaa aagtgccaat gaaagttgtg catgaaatac aagattttaa
     4441 agtgcctaaa ggtattgaag atcatttgtt cagaattaca caggaagcaa tttcgaatac
     4501 attgcgtcat tcaaacggta caaaagtgac agtagaattg tttaataaag acgattattt
     4561 attgttgaga attcaagata atggtaaagg ttttaatgtt gatgaaaaat tagaacaaag
     4621 ttatggactt aaaaatatgc gtgaaagagc tttggaaatt ggtgcaacgt tccatattgt
     4681 atcattgcca gattcaggta cacgtatcga ggtgaaagca cctttaaata aggaggattc
     4741 gtatgacgat taaagtattg tttgtggatg atcatgaaat ggtacgtata ggaatttcaa
     4801 gttatctatc aacgcaaagt gatattgaag tagttggtga aggcgcttct ggtaaagaag
     4861 caattgccaa agcccatgag ttgaagccag atttaatttt aatggattta cttatggatg
     4921 acatggatgg tgtagaagcg acgactcaga ttaaaaaaga tttaccgcaa attaaagtat
     4981 taatgttaac tagttttatt gaagataaag aggtatatcg tgcattagat gcaggtgtcg
     5041 atagttacat tttaaaaaca acaagtgcaa aagatatcgc cgatgcagtt cgtaaaactt
     5101 ctagaggaga atctgttttt gaaccggaag ttttagtgaa aatgcgtaac cgtatgaaaa
     5161 agcgcgcaga gttatatgaa atgcttacag aacgagaaat ggaaatatta ttattgattg
     5221 cgaaaggtta ctcaaatcaa gaaattgcta gtgcatcgca tattactatt aaaacggtta
     5281 agacacatgt gagtaacatt ttaagtaagt tagaagtgca agatagaaca caagctgtta
     5341 tctatgcatt ccaacataat ttaattcaat agttcatatc gaattaagaa aagttactta
     5401 cgccaatcac aatataacat caaatagaca ccaaaaaaca gaggttttcg caataatcat
     5461 tagcgaaaac ctctgttttg ttggtgttta gtctttttta agcttatcaa cgattttgtc
     5521 ttttatattt ttatcatctt ctggataggt atcactagga tgttcatcaa catgatacgt
     5581 acgttgatgt gttgcattac tatcttcatc atattcgtat ctagtgttct cattataatg
     5641 atttttatta ttgtcatcat gttctaatgc tgcttcttca ggtgttttac ctttaattac
     5701 actacgctga tgaatgattg cattgatttc agcaccgaca attataataa aacttgtgat
     5761 atataaccat agtaacaaaa tgatgatacc cgcgatactg ccatatgttt tagaatagtt
     5821 accaaaattt gaaatatacc aaccaaaacc aaatgaacca gctaaccaaa taattgaagt
     5881 aaatactgca cctggtaata ctgacttaag cttcgtttta acgttaggtg caaccgaata
     5941 taacacgata aatatgataa atataataat gattggtaac acaattctaa taaggttaaa
     6001 aatccatttc acttgttcgt caaatccaag tggaccgaat aggaaatgac taattacaga
     6061 accaagcgtt ggtaatgcta gagcaactac aaacacaacg cccataacta cagtgaagac
     6121 aacacttagt agttttaata cgattccatt acggctatct tctacatcgt aagcaacatt
     6181 gaaagaattc ataattgcag tcattccatt tgaagctgac caaattgcta aaatcaaacc
     6241 gatagataat aagccaccac tggagttttg agttatatca ccaattacac tcttaattag
     6301 agtagatgtt tcagcgggtg cattgcttaa catattagta atttgactct gcttaatatt
     6361 gaaaaatggt aataatgtta ataggaaaag caacataggg aacatagcaa gtacgaaatg
     6421 gtaagtcatt tgtgctgcta gtcctgaggc atcatctttt cctatgcgat agatgagata
     6481 tgataagaaa ttagagtctt tcttatattt tgcaggttta tttaatcttg ataagaagaa
     6541 aacctgatca tcttttttag gtttcttaga ttggaattgt tgaggttcaa tatatgtacg
     6601 atcaacatca attttgggat tacttttatt tttctttttt gactcctctt gcgcttcctt
     6661 aacagaatta aggtattttg aagaagagtg atcctttttc gacatgtgca ctccttaacc
     6721 tttctctatg taatgtttta taaagtaact attagtattt atccaattat aaaaaaacta
     6781 aacaatgata tgctttaaaa atgataatat tatagaaatt aattgattta aatctttcat
     6841 aggctatttt gatttaaaat taaaacaaag gcaatactga aacaatgcag tttggccaat
     6901 cattgtatta tcatttaata atgataacta tgaaaggcgt acaccaaatt gttaagtatt
     6961 gccttataaa aacaaaatca tcttagcgtt gattttttct attaacaaat gtttctttcg
     7021 catcctttaa tgaacgttct aattcaggat tattacgacg aatttcttca acaacatctt
     7081 tccagtataa aacttcgtct ttaatcttgc tgactttaga aggcttacga gtgcggttac
     7141 cattttttgc atctttaact gattgaacta aagcttgacg tgtagattta tcagctaaac
     7201 taattgcacc accaattacg gcaccaatta aaataccagg aacaaattta ttttccataa
     7261 aaaactaccc ctctttcaaa tttgcatctt ttacgatgta gtctattaaa ttatcacaag
     7321 atgataatac catgtcgtat acaccttcaa aattattcgt gtagtatgga tctggtacat
     7381 cactctcttc catattacta aattctaaca gtttgaacaa ttgtccctta agattaggat
     7441 tgatagattt aatattatca acgttacttt gatccatagc cacaatgtaa tcaaaatcat
     7501 ctgtcgcttc gaataattca ctaatcatgc catcaaatgg aatattgtgt ttgttgagaa
     7561 ttttttgtgt accttcatga ggtggctctc ctaaattcca gctaccagta cctcttgaat
     7621 gtactttaat atcatgaata tttctgtctt taagtctttg tcgcatgatt gcttctgcca
     7681 ttggagaacg acatatattg ccaagacaga caaatgctac atctaccatt ttgattcctc
     7741 caaactatgt agttatatcc ccattttata gcgactttaa acaataagaa agcagattat
     7801 ataaaattct attaaagttt attaaattgt gatactttga taacataact attattagag
     7861 gtgaacattg tggctatgac aaatgaagag aaagtattag ctattagaga gaagttaaat
     7921 attgttaatc aaggattatt agatcctgaa aaatataaaa atgcaaatga agaagaatta
     7981 acagatatat atgattttgt tcaatcaaga gaaagattgt cgccaagtga agtgacagct
     8041 attgctgacg ctttaggaca attgcgacac gaataggagt gggaattttg actaattaca
     8101 aagaaaagtt acaacaatac gctgaactat tagttaaggt tggtatgaat gttcaaccaa
     8161 aacagccagt atttattaga tcatcagtag aaacattaga attaacgcat ttaattgtag
     8221 aagaagcata tcattgtggc gcttcagatg tacgtgttgt atatagcgat ccaaccttaa
     8281 aacgcttaaa atttgaaaat gaatctgtgg aacattttgc taatcatgag ataaaatcat
     8341 atgatgttga agcgagaatg gattacgtta aacgtggcgc tgctaattta gcacttatta
     8401 gtgaagatcc tgatttaatg gatggtatcg atagccaaaa attacaagca tttcaacaac
     8461 aaaacgcacg cgcatttaaa ggatatatgg aaagtgtaca aaaaaatcaa tttccatggg
     8521 tcgtagctgc ttttccatct aaagcttggg cgaagagagt gtatcctgaa ctatctgtag
     8581 aagaagcata tattaagttt attgacgagg tattcgatat tgtgcgaatt gatggtaacg
     8641 atccagttga aaattggcgt caacacatcg ctaatttaag tgtttacgct caaaagttac
     8701 aacaaaaaaa ttaccatgcg ttgcattatg tatctgaagg tactgattta acagtaggtt
     8761 tagcgaaaaa tcatatctgg gaagatgcta caagttatgt taacggcaag gaacaagctt
     8821 ttattgctaa tataccaact gaagaagtat ttacagcacc agatcgaaat agagtagatg
     8881 gatatgttac taataaattg cctttaagtt ataatggcac aattatcgat caatttaaat
     8941 tgatgtttaa agatggagag attattgatt tttcagctgg aaaaggtgaa gcggtattga
     9001 aagatttaat caatactgat gaaggttcaa gaagattagg tgaagtagca ttagtacctg
     9061 atgattcacc gatatcaaat cgtaatacca ttttttacaa tactttattt gatgaaaatg
     9121 cagcatgtca tttagcgatt ggatctgctt acgcctttaa tattcaaggt ggaacggaaa
     9181 tgactgttga ggaaaagatt gcaagcggat taaatgattc aaatgtacat gtcgatttca
     9241 tgattggtag tagtgatttg actatttatg gcatattcga agatggttca aaagaactag
     9301 tatttgaaaa tggaaattgg gcatcaacat tttaataaat gttattttga ggtgctgagt
     9361 aggaaatgaa acatgtattt aaaggtcaaa tacgtgatta aagtatagat tgggagataa
     9421 aataatgaca aatcaggaca gaccaatgaa atctatgtca gaatcaaaat gttataaaaa
     9481 tagacaagtt ttccctcaag atacgaatca ccatcataca atgtttggtg gtacattgat
     9541 ggctaatatt gatgaaattg cagcaatcac agctatgaaa catgctggtg cgcaagtagt
     9601 taccgcatct acagactcag tagatttctt aaagccgatt aaaacagggg acatattaca
     9661 atacgtagcg atggtttcat acgctgggac tagttcaatg gaagtggtcg ttcaaattag
     9721 aattgacgat gtatttaata acaagcatga tttagctgca ttaagttact taacatttgt
     9781 tgcgttagat gatgaaggca aacctaaaca tgtacctggt gtatatccag aagatgatgt
     9841 tgaaaaatgg ttttatgata cagcaccaca acgtgttgaa cgaagaaaag cacgtcgcat
     9901 tgaaagtaaa caaacaattg aatatttagc tcaagtgcaa catattagag actaaaaata
     9961 taaagacaga ttgttaagga tgaattgaac tgttatgttc gaatattcca cctacaatct
    10021 gtctttttga ttatgcacgt ttatgctttt agttaaaatt tacccctttt attatagact
    10081 attagtccta ttaaatgccc tttattattt aagtagagta ctgccagcaa ttaattgttt
    10141 tttattttat aatagcatta aaatatgcgg agactactta tcgataaagt gcttctccgc
    10201 ataattaaga gtttttaaaa ttgtataatt tgtggttgtt tgtgcatatg acgctcatta
    10261 tctttaaaat ccatattcgg atagtacata tttttaacaa gaacatttgg tcctaaacaa
    10321 ctaaactcgg aacaatgaca atttaatgat ttagcaagat cagaggataa ccatttatca
    10381 aatacatctg ttaatttatc tttttgtata ttcgaaattg tacctgtttc atctccgaaa
    10441 tcagttacga ttacattacc tgtaaataca ttgacattta aacgactacg gccatccggg
    10501 tcatttctag tcgttacatt tttagcattt cttaaacgtg atagtaactt ttgatcttca
    10561 tcatccttta agcatggaaa cacaggcaaa gtaccaaata acatccaaat atcttcatct
    10621 ctgaaatcca atatatcatg aattgtcttt ttcatttccg ctagagttaa cacatttaat
    10681 tgacttgcaa agtcagctgg atacataggg tgaatctcgt gtctgctaca tttcatatca
    10741 tgaacgactt cttgatgtat ttttcgtaaa tgtggtagcg tactttgatt gagcattgtt
    10801 tccgcagata caaacattcc ttgttctgat aatgtacgtg cattcgaaat catttgttca
    10861 tataatttta acttagcttt taacggtggt tgcttcttca ttgcgccaaa gccaacattt
    10921 gcgaattcat cagttgttcc ccagttgtgt gagatatgca taacatcgat atattcagca
    10981 atatctaaat aacgatcttg aggcaatgtt aggtttgaat tcatttgtgt atatatacct
    11041 cgatgatgtg catactttaa tagaggttta acaacatttc taatagactt tttagaaaac
    11101 attggttcgc cacctgtaat agacatcgtt cgcagatttg gaatttcatc aagtctacga
    11161 taaattaagt ccatatctaa aggctccggg tcgacagttt gtaaagtata accaactgca
    11221 cagtggctac aacgcatatt acaaagattt gtagttgtaa attcgatgtt acttaaagtt
    11281 aattggccat gttctttaac atcgttatat
//
LOCUS       DANRDI010000087        11258 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.087, whole genome shotgun sequence.
ACCESSION   DANRDI010000087 DANRDI010000000
VERSION     DANRDI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.087"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..445
                     /locus_tag="PZ377_001737"
     CDS             <1..445
                     /locus_tag="PZ377_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDM6358608.1"
                     /translation="SDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            944..2431
                     /locus_tag="PZ377_001738"
     CDS             944..2431
                     /locus_tag="PZ377_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358609.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            2725..5355
                     /gene="polA"
                     /locus_tag="PZ377_001739"
     CDS             2725..5355
                     /gene="polA"
                     /locus_tag="PZ377_001739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDM6358610.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            5371..6243
                     /gene="mutM"
                     /locus_tag="PZ377_001740"
     CDS             5371..6243
                     /gene="mutM"
                     /locus_tag="PZ377_001740"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDM6358611.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            6259..6882
                     /locus_tag="PZ377_001741"
     CDS             6259..6882
                     /locus_tag="PZ377_001741"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDM6358612.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            7052..8077
                     /gene="gap"
                     /locus_tag="PZ377_001742"
     CDS             7052..8077
                     /gene="gap"
                     /locus_tag="PZ377_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDM6358613.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            8287..8757
                     /gene="nrdR"
                     /locus_tag="PZ377_001743"
     CDS             8287..8757
                     /gene="nrdR"
                     /locus_tag="PZ377_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDM6358614.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            8758..10158
                     /locus_tag="PZ377_001744"
     CDS             8758..10158
                     /locus_tag="PZ377_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HDM6358615.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            10158..11078
                     /gene="dnaI"
                     /locus_tag="PZ377_001745"
     CDS             10158..11078
                     /gene="dnaI"
                     /locus_tag="PZ377_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDM6358616.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            11171..>11258
                     /locus_tag="PZ377_001746"
     CDS             11171..>11258
                     /locus_tag="PZ377_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358617.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRY"
BASE COUNT         4117 a         1484 c         2067 g         3590 t
ORIGIN      
        1 atcagattta acgcggcgta ttattgataa tatgatgact caagccaatc aaaaaaatat
       61 ttccattcat actgatattg aaaaagatgt cattgttaaa gcacaagaaa gtaaaattgc
      121 tcaagttatc acgaatttgt taacgaatgc aattaactat tcttatgaag atggagatat
      181 taacgttcgt gtgtatcgag atgactttcg tgtcattttc gaagtacaag attttggtat
      241 aggtattaaa ttagaagacc aacaacgtat ttttgaaaga ttttatagag tagataaagc
      301 acgaagtaga gattctggtg ggacaggtct tggattatca attacaaagc atatagtaga
      361 ggcccaccaa ggcaatattg aagtgaatag tcaagttggc aaaggctcaa cgttcaaagt
      421 tattctaaaa gattataaag aataaaaatt aaagtggtaa cagcgcgtgt atttttcacg
      481 aagttgttgc tgctttttta tttcgttcaa atctctattg gtattaaatt agagttaatg
      541 aagtggaata aattcggttg tcaattttgt catttgtata tgtcgatttg taagttataa
      601 gtaatagatt gctgtgaatc aagcaatttt ataatatatt tattataata aagaaatttt
      661 agtaacgcgc ttgaaataga tgcttaatta aaaagtaata agttttattt gaacggaaat
      721 aaatcttaat ttaaacactt gaatagacaa gtgtaatcat tcgtcataga aaaagacgag
      781 gggcgattta gataaattgt tgaaagtaat attaaatacc ttgtttatct aatttgattg
      841 aggaaacatg cataggtgaa aattagaaat acattaaaaa tattgaatta atttttcgac
      901 ctacaagttg aggaaaattt aactagattt gaggattagc aaagtgaaaa caattcaatt
      961 acacaagaca actttgatat taattgcgat attattgttt tatactttta tgggtatctt
     1021 actaccactc atgcacgatg atttacaatg gtttagcaat tataatactg atattttaaa
     1081 agtaggattt gcatcactta atggtcgcta tatcggaaat atctttgaaa ttatagctgt
     1141 acatgtaagc tggttacgtt ggctttcata tggcctcatt agtatgggca tcatttggat
     1201 gattatgcac attacacgtt gtaaagcgtg gacaagctat tatttgttag cattttcttt
     1261 aatgttaatt ttaccaagtg ctatttatgc agatacatat ggctggtttg caggatttta
     1321 taattatgcg acatcaacac taatttcact ctttattatt tattattgta ttaatgcaat
     1381 tatatataaa gaaaagcagc cggtaagtgt tactgtacta ttctatgttt tatgcttctt
     1441 cggtcaatta tttatggaga atgtgacatt attccactgt atcattttaa ttttagcttt
     1501 tctgtatgaa tttactgcaa atcgaacact taattataaa ttattgtttt catttatgat
     1561 tgctactata ggtacaatca ttatgttttc caatccaaat tatcgcaaaa ttttatttga
     1621 aggatcagaa tatcaacaag tttcaaataa tcaaggtatc ttttcaaaat ttgcagaaat
     1681 gatatcaacg tcacttccat atggtgtcat tttcagtcag attataattt taagtatgat
     1741 tgcagcgctt attatttatc tactgcttag aagtgatcgg tatgtacatt taacaatatt
     1801 aaagcgtcgt atcattatga taggatttat cactttaccg ctttattatt tactatttta
     1861 caatcagttt ttattaaata aaaatacaga tattggattg gttggttttg tgaacgtaat
     1921 tgtatgtggt tactttgcgt tttcgatatt tgtaggtatt tatttgtcta ttaatgatcg
     1981 taaaacacaa atcactttat attccttact tattgcaata tgtgtttcgg cgtcgacact
     2041 agtcattgtt actccaatag aaccaggtaa ttttttgatt gtttatacga tacatgtaat
     2101 cattttaatt attttattaa aagaatttcg gaaatataag tcaatcaatg ttaattttat
     2161 taaaggtgca tcactagttt tagctgtgat atatcttagt gcatttattt atgttcatta
     2221 tgaacatgaa aaaagagttc agttattaaa acaacaaatt aaagatcatc cacgacaaga
     2281 ggtttataca gtggaatcat tgccgtttga acattatatg catcatccgt caccaatcaa
     2341 taaaaattac caagagtggt ttaataatta tgaagaatta ccgaaaatga ccaaagttaa
     2401 atatattcca tttggttcag acgattcata acttattctt acctataaaa atttaaaata
     2461 gcagcataaa aatattaaaa agtagacata atcatgaaat gataatgtgc atctcaaaag
     2521 ttggactttt aacctgactt tatggagtgc tcatcaagaa caatcatgat tatgtctttt
     2581 ttatttttta gctaagtcta tataggcact tgttttattt gaaattaggt cgtgtataaa
     2641 tactctatga gatagtttct ttaattacga catcacagtc gaatatgcta aaatatatat
     2701 aaattagttc tggaggttaa aattgtgaat aaattagtat taatcgatgg taatagctta
     2761 agttttagag ctttttatgc attacctttg ttatcaaaca aagcaggcat tcataccaat
     2821 gcagtatatg gttttgcaat gttactagag aagattttaa aagaagaaaa gccaaatcat
     2881 tttttagtag cgtttgatgc aggtaaaacg actttcagac atgaaaaata tagtgaatat
     2941 aaaggtggac gccagaaaac gccgcctgaa ctaagtgagc aatttcctta tattcgccaa
     3001 ttattagatg cttatcatat taaacgttat gaattagata actatgaggc agatgatatt
     3061 atcggaactt taagtaaaga agcagataaa gcgggctttc agacaattat tattacggga
     3121 gaccgagatt taacacaact tgcaacggat aatgtaacca tttattacac taaaaaaggt
     3181 gttactgatg ttgatcatta tacacctgac tttattgcgg aaaaatacaa cgggttaaca
     3241 cctaatcaaa ttattgatat gaaaggatta atgggagata cttctgataa tataccaggt
     3301 gttgcaggtg ttggagagaa aacagcaata aaattgctaa accaatttga cacagtagaa
     3361 ggtgtctatg aacatttaga tgaaatttca ggtaaaaagt taaaagaaaa acttcaaaat
     3421 agcaaagaag atgcattaat gagtaaagaa ttagcaacga ttaatgtaga tagtccgatt
     3481 gaagtaaaac ttgaagatac gttaatgact catcaagatg aacaacaaga aaaaatcgaa
     3541 ttgtttaaga agttagaatt caaacaattg ttggctgaca ttgatcaatc agcaagcgtt
     3601 gaagatgcaa tagaaaagac atttgaaatt gaaacgtctt ttgataatat tgattttact
     3661 tcattgaaag aagcggccat ccatttcgaa ttagacggtg gtaactattt gcgaaataat
     3721 attttaaaat tctctttatt tacaggtgag aaacatattg taattaatgc ggatgacata
     3781 aataattatg tcgaactagt ttcatggtta gaaaatccga attcgaaaaa agtcgtatat
     3841 gatgctaaaa aaacatatgt agcatcacat agattgggga ttgatattca aaatatttct
     3901 ttcgatatta tgttggccag ttatataatt gatccatctc gtacgattag tgatgttcaa
     3961 tcagttgttt cattgtacgg tcaaagtttt gtgaaagacg atgtgagtat atatggaaaa
     4021 ggtaagaaat ttaaggtacc tgaagatgat gttttaaatc catatgttgc ttctattact
     4081 gatgcaattt actttgcgaa accaaatatg gataaacaat tagaagaata caatcaggta
     4141 gaactcttgg ctgatttaga gctaccgcta gctaagattt taagtgaaat ggaagaaatt
     4201 ggtatattta cagatgttca tgatttagaa gaaatggaaa aagaaattca agaaaaatta
     4261 gacgtcttga ttcgaaatat ccatgatgca gctggtgaag attttaatat aaattctcct
     4321 aagcaattag gtgttgtatt gtttgagaca ttacaattac ctgttattaa aaagacgaaa
     4381 acaggatatt ctactgctgt agatgtctta gagcaattgc aaggtgaaca tcctatcatt
     4441 gattatattt tagaatatcg tcaattatct aagttacagt ctacttatgt tgaaggattg
     4501 caaaaggtaa ttagtgatga tcaacgtatc catacacgtt ttaatcaaac tttagcgcaa
     4561 actggacgtt tatcaagtgt agatcctaat ttacaaaata ttccggttag acttgaagaa
     4621 gggcgtaaaa taagaaaagc ctttaaacca acttcaaaag atagcgttat attatcagca
     4681 gattattctc aaattgaatt gcgtgtatta gcacacatta cacaagatga gagtatgaaa
     4741 gaagcattta tcaacggcga tgatattcat acagcaactg ctatgaaagt atttggtgta
     4801 gaagctgatc aagtcgatag tttaatgcgt cgtcaagcaa aagcagttaa ctttggaatt
     4861 gtttatggga taagtgatta tggtttaagt caaagtttag gtattactcg taaaaaagca
     4921 aaagcattca ttgatgatta tttagctagt ttcccaggtg taaaacaata tatgtctgat
     4981 attgtaaaag atgccaaagc tttaggttac gtggaaacat tgctacatcg tcgacgctat
     5041 attcctgata ttacgagtcg taactttaat ttacgcggct ttgctgaacg tactgctatg
     5101 aatacgccaa tacagggcag tgctgcagat atcattaaac tggcaatggt taaatttgct
     5161 caaaaaatga aagagacaac atatcaagct aaactattat tacaagtaca cgatgaatta
     5221 atttttgaag tacctaagtc agaagtagat tcatttagtg aatttgtaga agagataatg
     5281 gaaaatgcat tgcaattaga tgttccatta aaagtagact caagttatgg tgcaacctgg
     5341 tatgatgcaa aataaagtga ggttaatggt atgcccgaat taccagaagt agaacatgta
     5401 aaaagaggaa ttgaacccta cgttataaat caaaaaattg aacacgttat attttcggat
     5461 aaagtaatcg aaggtaaagc tcaaggcaag gaaacaatta taaaaggaat tgaattagat
     5521 acttttaaaa ctttatcgga aggctatacc attacaaatg tagaacgaag aagtaaatat
     5581 atcgtttttc agctagataa taaacgagaa caacgtacat taattagtca tttaggtatg
     5641 gcgggcggtt ttttcattgt agatgaactt gaagatatta tgatacctaa ttatcgtaag
     5701 cattggcatg ttatttttga gttgtcaaat gacaaaaagt taatttattc agatatacgt
     5761 cgttttggag aaattagaaa tgttgcgtct gtagcatctt atccttcatt tttagaaata
     5821 gcgcctgaac ctttttcaaa tgaagcattg acgtattatt taaatcgaat tcatcaacag
     5881 tctaataaga ataaaccaat caaacaagtg attttggatc ataaagtaat cgcaggttgt
     5941 ggaaatattt acgcatgtga agcgttgttt cgtgcaggtg ttttaccaga taaaaaagta
     6001 aaagacttaa cacaccaaca acaagaaatg gttttttatt atgttagaga agtactagaa
     6061 gaaggtatta aatatggcgg tacaagtatt tcagattatc gtcatgctga tggaaaaact
     6121 ggtgagatgc aattgcatct taatgtatat aagcagccgg tatgcaaagt gtgtggaagt
     6181 caaattgaaa caaaaatcat cgcgacaaga aatagtcatt attgtccagt atgtcaaaaa
     6241 taaagaaaga gtgaaattat gccgaaagtt attggtctaa caggtggaat cgcctcagga
     6301 aaatcaacag tatcagaact cttatccgta ttcggtttta aagtagtaga tgctgataaa
     6361 gcagccaggg aagctgttaa aaaggggagt aaaggtttag ctcaagtacg agaagtcttt
     6421 ggtgatgaag caattgatga aaatggtgag atgaatcgtc gttatatggg tgatctagtg
     6481 tttaatcatc cagaaaaacg cttagaatta aatgctatca tacatcctat cgtgcgagat
     6541 attatggaag aagaaaagca agaatattta aaacaaggat ataatgtaat catggatatt
     6601 ccattattat ttgaaaatga attggaaaat acagtagacg aagtgtgggt tgtatacact
     6661 tctgaaagta tacaaatgga tcgtttaatg caacgtaata atttgtcatt agaagatgcg
     6721 aaagcacgtg tctatagcca aatttctatt gataaaaaaa gccgaatggc cgatcatgtt
     6781 atcgataatt taggggataa acttgaatta aaacaaaacc ttgagagatt gttagaagaa
     6841 gaaggttata ttgaaaagcc gaattacgga gaagaagatt aatattacac tataaataag
     6901 tcattacttt acgtacgcgt tgatgtatgt aagtaatgac tattttttat aaaaaagata
     6961 aataaatcaa cggaaaacgc tttcaaattt catataatat gctatactaa ttccataaag
     7021 tataacacat aaagatcaag gggtgctttt aatgtcaacg aatattgcaa ttaatggtat
     7081 gggtagaatt ggaagaatgg tattacgtat tgcattacaa aataaaaatt taaatgtagt
     7141 agcgataaat gctagttatc cacccgaaac aattgcacat ttaatcaatt acgatacgac
     7201 acatggaaaa tataatctaa aagttgaacc gattgaaaat ggattgcaag ttggagatca
     7261 taaaattaaa ttggttgctg atcgcaatcc tgaaaacttg ccatggaaag aattagatat
     7321 cgatattgct atagatgcaa ctggtaaatt taatcatggt gataaagcca tcgcacatat
     7381 taaagcaggt gccaaaaaag ttttgttaac tggtccttca aaaggtggac atgttcaaat
     7441 ggtagttaaa ggcgtaaatg ataaccaatt agatatagaa gcatttgaca tttttagtaa
     7501 tgcttcatgt actactaatt gcattggtcc agttgcaaaa gttttaaata atcagtttgg
     7561 aatagttaat ggtttaatga ctactgttca cgctattaca aatgaccaaa aaaatattga
     7621 taatccacat aaagatttaa gacgtgcacg ttcatgtaat gaaagcatta ttcctacttc
     7681 tactggtgcg gcgaaagctt taaaagaagt attaccagaa ttagaaggta aattacacgg
     7741 catggcatta cgtgtaccaa caaagaatgt atcgctcgtt gatttagttg ttgatttaga
     7801 aaaagaagta actgcagaag aagtaaacca agcttttgaa aatgcaggtt tagaaggtat
     7861 catagaagtc gaacatcaac cactagtgtc tgttgatttt aatactaatc caaattcagc
     7921 tattattgat gcaaaatcaa caatggtcat gtcagggaat aaagtaaaag ttattgcttg
     7981 gtatgataat gaatggggtt attcaaatag agttgtagat gttgctgagc aaattggagc
     8041 acttttgaca tcaaaagaaa ctgtaagtgc aagttaatac aattaattta tagcctaact
     8101 ctgacttcgg agttaggttt tttattttta atacaataat attgtcatgc aaattactta
     8161 ataagtaacg atataatgtt ttttattgtt tcattatttg gttaaatgaa ttaaattgag
     8221 ggattctttt ttcatctcta gatttatagt ataattaggc ataatcaaaa taaagagggt
     8281 gatgtaatga aatgcccgaa atgtaattct acacaatcta aagttgtaga ttcaaggcat
     8341 gccgatgaat taaatgccat tcgaagacga agagaatgtg aaaattgtgg aacacgtttc
     8401 actacatttg aacatatcga agttagtcag cttatagttg tgaaaaaaga tggcacaaga
     8461 gagcagtttt caagagaaaa gatacttaat ggacttgtgc gttcttgtga gaaacgacca
     8521 gttagatatc aacaacttga agacataact aacaaggttg aatggcaatt acgagatgaa
     8581 ggtcatacgg aagtgtcttc acgagatata ggtgaacacg ttatgaactt gttaatgcat
     8641 gttgatcaag tttcatatgt tagatttgca tcagtctata aagaatttaa agatgttgat
     8701 caattgttag catcgatgca agggatttta agcgaaaaca aacggagtga tgcttaaatg
     8761 ggacgacaag ccttcgaatt cggcttaaga ccaaaggatc aattcaaagt tatgcaacac
     8821 tttgatttaa atacgaatca tttagaggta ttgaatcgac tgtatacacc gctaattgga
     8881 acgcaagcag tgggtttata tcattttatg actcaattcg ttaaagaaag tcataatgaa
     8941 acattgattc tttctcatta tatttttatg aatgaactta aaattaattt actagagttt
     9001 agacaacaaa tggacttact tgaagcgatt ggattgttaa aggcgtttgt caaacatgat
     9061 gaacaagaaa cacaatttgt ttaccaatta attcaaccgc cttcagcaca tttatttttt
     9121 aatgatccaa tgttgtcaat ctttttatat agtgaagtcg agcatcgtcg atttcatgag
     9181 ttgaagaaat actttgagta tcaacaaatt gatttgagtg aatttaaaca ggtaacaaga
     9241 caatttactg atgtttttaa agtgccgtct acaaaaatag atatcgatac aagtgatatt
     9301 ccgattaatg aaccgtacca aggtatcgat ttatcaaatg aatcttttga ctttgaaatg
     9361 ttgagacaaa tgttaggtaa acattttatt agtcaagaca ttgttaccaa agatgcaaag
     9421 cgtttaatta cacaactcgc gactttatat ggtttaacag cagatggcat gaagcatgtt
     9481 attttaaatt caattacaag tggacaacag ttatcctttg aagaaatgcg taaacaagca
     9541 cgctcgtatt acttaatgga acacgaaaat caaatgccta aattacaggt gaaatctcct
     9601 gctacatcat cttcggcggg caaatcttca gaagtgaatc caaagccaca aagtgatgaa
     9661 tggtttgagt tacttgaaca aacgagtcca attgatatgt tagcttcttg gtctgaatca
     9721 gaaccaacta tatcgcaaaa gacaatggtt gaagaattaa ttgaacgtga aaaaatgtct
     9781 tttggcgtca ttaatatttt gttgcaattt gtcatgttaa aagaagatat gaaacttcct
     9841 aaagcgtata ttttggaaat agcatcaaat tggaagaaaa aaggtattaa gacagctaaa
     9901 gaagcatata attacgctaa aaaagttaat caacctaaaa atgagggttc tagcggaaat
     9961 taccaaaagc gtggttcata ctatgggcag cgtaatcgta tttcaaaaga aaaaacacct
    10021 aaatggttag aaaatagaga taaacctagt gaagaagatt cggctaaaga taatagcgta
    10081 gatgatcaac aattagagca agatcgacaa gcatttctag ataaattatc taaaaaatgg
    10141 gaggaggaca gtcaataatg aagcaattta aaagtataat taacacgtcg caggactttg
    10201 aaaaaagaat agaaaagata aaaaaagaag taatcaatga cccagatgtt aagcaatttt
    10261 tggaagcgca tcgagctgaa ttaacgaatg ctatgattga tgaagactta aatgtgttac
    10321 aagagtataa agatcaacaa aaacattatg acggtcataa atttgctgat tgtccaaatt
    10381 tcgtaaaggg gcatgtgcct gagttatatg ttgataataa ccgaattaaa atacgctatt
    10441 tacaatgccc atgtaaaatc aagtacgacg aagaacgctt tgaagctgag ctaattacat
    10501 ctcatcatat gcaacgagat actttaaatg ccaaattgaa agatatttat atgaatcatc
    10561 gagaccgtct tgatgtagct atggcagcag atgatatttg tacagcaata actaatgggg
    10621 aacaagtgaa aggcctttac ctttatggtc catttgggac aggtaaatct tttattctag
    10681 gtgcaattgc gaatcagctc aaatctaaga aggtacgttc gacaattatt tatttaccgg
    10741 aatttattag aacattaaaa ggtggcttta aagatggttc ttttgaaaag aaattacatc
    10801 gcgtaagaga agcaaacatt ttaatgcttg atgatattgg ggctgaagaa gtgactccat
    10861 gggtgagaga tgaggtaatt ggacctttgc tacattatcg aatggttcat gaattaccaa
    10921 cattctttag ttctaatttt gactatagtg aattggaaca tcatttagcg atgactcgtg
    10981 atggtgaaga gaagactaaa gcagcacgta ttattgaacg tgtcaaatct ttgtcaacac
    11041 catacttttt atcaggagaa aatttcagaa acaattgaat tttaaaatga ttggtgtata
    11101 atgaatacaa atctaaatcg tttaaatgat tgaagacaag atgatctaat caatattaca
    11161 cagaaagcca ttgtttgatg agaatatggt taataaatta gatgattact acttcattta
    11221 tggtatttgt aatgaatacc cggatcaaga ccgttatc
//
LOCUS       DANRDI010000088        10997 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.088, whole genome shotgun sequence.
ACCESSION   DANRDI010000088 DANRDI010000000
VERSION     DANRDI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.088"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            46..360
                     /locus_tag="PZ377_001747"
     CDS             46..360
                     /locus_tag="PZ377_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358618.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(653..1456)
                     /locus_tag="PZ377_001748"
     CDS             complement(653..1456)
                     /locus_tag="PZ377_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDM6358619.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(1514..2428)
                     /locus_tag="PZ377_001749"
     CDS             complement(1514..2428)
                     /locus_tag="PZ377_001749"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDM6358620.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            complement(2430..3491)
                     /locus_tag="PZ377_001750"
     CDS             complement(2430..3491)
                     /locus_tag="PZ377_001750"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDM6358621.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(3497..4777)
                     /locus_tag="PZ377_001751"
     CDS             complement(3497..4777)
                     /locus_tag="PZ377_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDM6358622.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            4968..6350
                     /locus_tag="PZ377_001752"
     CDS             4968..6350
                     /locus_tag="PZ377_001752"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDM6358623.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(6404..6979)
                     /locus_tag="PZ377_001753"
     CDS             complement(6404..6979)
                     /locus_tag="PZ377_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358624.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            7310..8164
                     /locus_tag="PZ377_001754"
     CDS             7310..8164
                     /locus_tag="PZ377_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358625.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            complement(8307..8840)
                     /locus_tag="PZ377_001755"
     CDS             complement(8307..8840)
                     /locus_tag="PZ377_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDM6358626.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            8979..9167
                     /locus_tag="PZ377_001756"
     CDS             8979..9167
                     /locus_tag="PZ377_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDM6358627.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(9280..9882)
                     /locus_tag="PZ377_001757"
     CDS             complement(9280..9882)
                     /locus_tag="PZ377_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6358628.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(9879..10970)
                     /locus_tag="PZ377_001758"
     CDS             complement(9879..10970)
                     /locus_tag="PZ377_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDM6358629.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
BASE COUNT         3661 a         1895 c         1497 g         3944 t
ORIGIN      
        1 tcgtgtataa tatagataac aataactttt caggggtgaa attcaatgat tggacaaaca
       61 aatttatttg atgatgtact aaagcaagat gaaagatttg ttattgtagt tcaagcatta
      121 gaagaaaaga acggacaatt attaaagaga actttaaggg aatatcccgg tttaaaccat
      181 aaacaaatga atgatttatt tatgcactta aaggaattat tttccgaaga atcatttgct
      241 gaaaaccaat cagcgttcag tattacagtt tatacaaatt tagattatac tgctgaccaa
      301 atatatgctc atgtaaaacg tttcagaggt aagcatgact ggacacaaac agctaaataa
      361 aatcaagatg cttgcagtga ttttaacaat taatgttaga ttgtcactgc attttttaat
      421 gcattcgtta ttattcagaa tgatcaaatt tatttatcta ccttccgtga ctagttaata
      481 cttgatttat tcaaaaaata tacatttact ctattcgttt gatatcatta ctaatctaaa
      541 atatgtattt caaatcctcc atagtataac tgaagtcatt ttcacaaaaa taaaaagcct
      601 taaccacaag atatcttatt caaatatcat gtgttaaggc ttattttaaa atttataaca
      661 attttttgag cgcatgtgga ataccatttt cattatgatc caacgtaact tcatttgcat
      721 attgcttaag ttcatctgat gcattcccca tagcaatagc tagacctgcg acttcaaaca
      781 ttgatttatc attcatacta tcaccaaaag caatgacttg atccaccgaa tatcccaatt
      841 tgtgacataa cgctttaatt gcattacctt ttgaaacgtc tttggccata aattctaaga
      901 agaatggctt acttgtcgta gcatctacat tatcattgaa cacaccattc aaatcaattc
      961 tagcttctgt aatattcgct acataatcta cacccatgac cttgggtaca tcgccttgaa
     1021 tatacgcttt gatatcatca acacgtttca tcggtaaacc tgttaattct gcttcaatat
     1081 tcatatactc atgttcgctg tcgtaaataa tttgaccatc atgatatgta agaacgaaaa
     1141 agcctctatc tcgacaataa tctacaattt catcgaaatc ttgcttacca atcgatttac
     1201 ttacttctac ttcttcatta gtcatgttaa tcgttttact accgttataa ctaataatat
     1261 agctatgatg ttcaggtaat tttaaatctc tagcagttgg aatcatacct tcagtaggtc
     1321 taccagatgc tagaacaaca taataacctt catcttgaat tgctgttaaa taatttgcag
     1381 tttcttctga tatcacatta tctgaattta gcaatgtgtc atccatatct aaaacgacaa
     1441 ctttataatt tgtcatatcg cattaaatct cctctctatt ctgcatttaa tactatttta
     1501 caatataaag cgtctattgg tatacaattc gttctttaac accactgata ttaatatcga
     1561 ccaattcaga atggcatgat actacctgac tatttaaaga gcgaaccaat tccccactat
     1621 tttctttacg actaaatatt aaaatagttg gtccagcacc actaattaca gttgcatagg
     1681 cattatgttg actagcaatt gttttcactt catcaaattc agcaattaaa tgctgacgat
     1741 acggttcatg aaagccatct tgttgcatga gtttacctgc taattcataa ttgtgctgtg
     1801 ctaatgcaca aatcattgta ttactaattg cactactttt aaccgcttca ctatgtgtta
     1861 atttttgtgg taaagcacgt cttgatgctt ctgtttttag ttcataagtt ggtatcgtta
     1921 caatcacatc cacgtctggt atgtcgatat gtgcaactga cgtttctttc gagacatcat
     1981 tataatatcc agcgattaaa ccaccataaa tggtcggcgc aacattatca ggatgtcctt
     2041 cgatttcagt cgctaattgt aatacctcat gtttagacag ttggatatca ccaaaataat
     2101 ttgcgatata tatagctcct actaaagcag aagctgacga acctaaccct cttgccaatg
     2161 gaatatcact tctcatttcg atacataaat taggcaagtc aacactatat ttagaagcaa
     2221 cttgttgtgc tacatgataa ataaagtttg tttcgtcagt aggcaattgc ttagatgcat
     2281 catcatggaa aatatattcc cattttgtcc ctgatgtttc ctttacagac agatgcaaaa
     2341 atttatctaa agccatacct atagaatcaa agccaactcc aaggttggct gttgatgcag
     2401 gaattgttaa ctccaaaaca ttcgacatgt tataaagctc ctttaatata atcgataatg
     2461 ctatctttat catttggcaa tggctttatc ggattatcta gtagtgaaat agcagtatca
     2521 ggatctttta atccattacc agttaaaata gcaactactt ttttaccttg aggtaattta
     2581 ccttgtctat gcaatttaat taaacctgca atagaagcat tactcgctgg ttcactaaag
     2641 acaccttctt tagttgtcat taactgatat gcttctagaa tttcatcatc agtaacacta
     2701 tctattaatc catttgattc tttaagagca ttagtcgcct tatcccaact agcaggatta
     2761 ccaattcgaa tagcagttgc aatcgtttca ggatttttaa tgactttatt ttgaacaatt
     2821 ggtgatgcgc cttcagcttg gaagccaaac attttcggca attgtgatcc tttagcttca
     2881 tgatattctt taaagccttt ccaatatgca gtaatattac ctgcattacc tacaggaatc
     2941 gctaaaatat caggtgcttc accgtctaat tgttgtacaa tttcaaatga gcctgtcttt
     3001 tgtccttcga ttctaaatgg attgacagag tttacaagct cgatttcgcc actttttgca
     3061 atttctttta caatttctaa agcttcatca aagtttcctt caatagaaac gatttctgca
     3121 ccatacatta ctgcttgcga caatttacct aatgcaattt taccttctgg tattacgacg
     3181 atagctttta aacctgctct cgctgcatat gctgctgcag acgctgatgt atttccagtc
     3241 gaagcgcata ttacaatttt cttaccttgc tcttttgctt ttgtcacagc cattaccata
     3301 ccgcgatctt taaatgaacc tgtcggattg gcaccttcat acttcacata taaatctatg
     3361 cctagtattt tagacatatt ttcacaatga atgagtggtg tatttccctc gttcaatgtt
     3421 aattttggtg tattttcatt tactggtaaa tgtgctttaa actcttctac taatccttgc
     3481 catcttctca ttataattaa actccttcta ctgggtatac tttgtcaact tcgtatccat
     3541 gcttagtgat taattcttcc ggtgatgatt ccaatccaac tataacagcg gcatatgatt
     3601 gattatcgta atttgcaact cttaatgatc ggtgaaatgg taatattgcc tttaactcat
     3661 tttcaaaatt ttcaattgaa cctttgacat gattcactac tacaaagaaa ttggattttt
     3721 ctttaatatt aatttctgca tctgaatcca tcatttcccg tgttttatct gtctttaatt
     3781 caaaatgtgg tggcaatgtg tgtaaatctg attcaaagaa taatgctaca ttcaataaat
     3841 cactgacaac ggcactacct gttgctaaac tgcctgctcc ttttccataa aacatcgtgt
     3901 caccaacggc atcaccaata acataaatcg cgttatattc atcctctaca gctgctaatt
     3961 gatgcttttt atcaattaac gttggttcta ccgatgcatt aactttgcca ttttcatata
     4021 ttcccttacc aatcaattta attttatacc ccagttgatc ggctacatta atatcagtta
     4081 aagttacgcc actaattcct cttcgtttaa cgtcgtttaa tttaatgact tggttaaatg
     4141 ataaatatga tgtaatgaca actttacgcg ctgcatctac cccttctaca tcatcagttg
     4201 gatccgcttc agcaaaacca agtcttttcg cttcatcaag tgcttcctca aatgtcgttt
     4261 gctctttagt cattttagat aaaataaaat tagaggtacc atttaaaatt cccataaatt
     4321 ttgaaatatt attcgcattc aaaccattat ttatggcatt tacgatcgga ataccacctg
     4381 ctacactcgc ttcaaacttt aaagctacac cattttcttc tgctaaatct tctaaaagtt
     4441 taagatgtac tgctaataaa tctttatttg cggtaataac atgtttttta tttttaagtg
     4501 ctgttcttaa ccaatctaca gttggttcaa ttcctcccat gacttcaacg ataatatcta
     4561 atgaatcatc atttaaaatt tcattaacat cttcagttaa atgatattgg ctaatattta
     4621 gcggtctctt tttagattta tctcgaacaa gaatatgctt tatgacaata tctttattta
     4681 atgtatcttg aatttgctgt cggttctctt cgatgatttt aacaacacca gatccgacag
     4741 tacctaatcc taataatgct atatttaatt ttttcatttt atgtacctcc gtttgtgctt
     4801 ttgagaaaaa caaatgtatt attgaaaaac actagatgat agtatagtat agaattattc
     4861 tttatttttg taaagttcgt aatattcaaa atataaatac ataatatcat tcttacttac
     4921 aaaactgaat acaattttct gattttttag aaaggttggc atgcactatg aaagtttcca
     4981 aattcggtgg tagctccgtc tccaatgcta ctcaaattaa aaaggtttta aatattgtta
     5041 attctgatcc agagcgaaag attgttatcg tttctgctcc aggtaaaaga catgataatg
     5101 atattaaaac aactgatttg ttaatcagat tatatgaaaa ggtcattaat catcttgatt
     5161 atcatgataa aaaaagagaa attattcagc gttatgatga tattgtaaaa gaattgcaaa
     5221 tggatgaaag tattttacgg acgatagatg tgactttgga acattatatt aatcaattaa
     5281 aaaatgaacc aaagagacta ttagatgcat tactttcttg tggtgaagat tttaatgcgc
     5341 aattgatagc tttatataat aatagtcaag gtataccaac aaaatatata tcaccgaagg
     5401 aagcgggtat ttttgttact gacgtcccac aagaagcaca aatattagat agtgcttact
     5461 ctgaaattaa aaaattaaga gattatgatg aaaaactaat tatcccagga ttttttggcg
     5521 tatctcatga aggatatata gttacatttc cacgtggcgg atcagatata actggcgcca
     5581 tcatatcaag tggtgttaga gccacaattt atgaaaattt caccgatgtg tctggtattt
     5641 ataaagctaa tccaaatatc atcaaagatc ctgaattaat cgaagaaatc acttatcgag
     5701 aaatgcgtga attatcttat gctggtttta gtgttttcca tgatgaagcc ttacaaccac
     5761 tttataaaga tcgcatccct gtggtgatta aaaatacgaa ccgccctcaa gataagggta
     5821 catttatagt gcatgataga gaaataaatg cgaaaaatgt gattagcggt ataagttgtg
     5881 ataaagattt taccgttatt aatatcaaaa agtatttaat gaatagacaa gtcggcttta
     5941 ctagaaagat attaggtgtc ttagaggata ataatatttc atttgatcat atgccttcag
     6001 gtattgatac tatcagtatc gttatgcgtt caaaacaaat tcaaaataaa gaaacaaaag
     6061 tattaaatga aattcgtcaa aaatgtgatg ttgatgagct aagtattgag catgatttag
     6121 caatattaat gattgtcggc gaaggtatga ataaagtcat cggtactgca aataaaatta
     6181 cacatgcctt agctgaatca aaaattaact taaaaatgat taaccaaggt gcttcggaaa
     6241 tctcgatgat gtttggaatt catgtagatg atgctgaaaa agctgtatta tctacatatg
     6301 agttttgcta tcacggggtt gcattaaaaa acttgtgtga taaaaactaa tataaaagca
     6361 attcgtaaca catgtattca actgcgttat gaattgcttt ttcttaatta tttattgttc
     6421 tctggattgc atccttaata cgttgctcac ctactacaaa ttttaaaata aaaaattgat
     6481 agtagcttaa tgcatctatg actcgtttat aattggaata atgatgatac tgatggtgtt
     6541 gcattagatc atacattaga acgatctccg gtttaatata atccacatcc caatttaaac
     6601 tatggaagta tatgttactc ttaggtatac gtatattcgg atccaatcta aaaatccatt
     6661 catcgtttac tacatcataa acgaaaatac tcattattgt tgtatcatca tattgcacat
     6721 aaacttttgc aatttttgaa agtgataaat cttctttagc tattgcttga tcttgcttat
     6781 ctatgtatag taacgcatat gattgcggaa taactttcac aatatcactt aaacgcttac
     6841 gttccacgca tatttcaaca atagaaggta attttaattc atcatttaag aaaagctgca
     6901 tggcaacttc accgtgaaat ttaaataatt gcgatgatgt tttcattaaa ttgtccacta
     6961 attgttcttt agcattcatt tcattcactc tcaaatacac ctcctaagca agcgctttca
     7021 caaatatcat tatacatttt aaaattaatg aattcaatct tcaaaatact atgagtgacc
     7081 taattctgtc atttaaatga ccgttataac ataaattcga cataaacttg aattcacgaa
     7141 tctttcacgt catattcatt gaacatcaat atctgtcagt ttataatttg attataacaa
     7201 tgatgaacta aaatcaacta ataaaaatgt gggaaattga ataacaatta atctttatta
     7261 attattattg tatacaaagt taaagaagtc aaaaaggatt ggtgaaaaaa tgtcttttta
     7321 tgttgtactt atcattatca ttgtagcatt aattggtata ttagttttaa atcaaaggta
     7381 tagtaatagt aaaatagata ccgaagttta tgcaagaaag caacttatta aaaagaacaa
     7441 agcattaagt gcagaaaatg ctgaattaag aagtcaaatg ctgagttcta ataatgatgt
     7501 tggccaccat gcatacaaaa atgctaaaag agaattacgc aagattttag atagctatct
     7561 cgaaaatggt aaattaaaat attacgatat tatcgttaca agcaatttag ctactaaaca
     7621 tccgtttttc gagtatgcac gttcatttga ttttatcatt gtttcagata tcggtttaat
     7681 aaacgttgat gtaaaaagct ggggtgaaaa aacattttat cactttgatg tacctgatga
     7741 gcatgataca gaaataagca atagtaatat tgaaaaagtt gtcggtcatt acattagcca
     7801 gcaatatcat gatcagttta attcatcaag aaagtctatc tatactttta cagaaacagt
     7861 tcaaccaaat cgtgttattt atgattttta tgattatgat ccgtatcaat tagcagcaaa
     7921 taatgcgaaa gcattaaagg atcatattga acaaaacttt aactttaaag tccaaagtac
     7981 cggtgttatt tactttagtg atggtacagt taatatcatt caaggttctg aagaaagaga
     8041 taaatatgtt gatacagttt caacaaaatc ttcgttacga cgtattatta gcgaagccat
     8101 agagctatct aaacaccctt taaataaaga acaggttgat caaatcacag ctatattcaa
     8161 gtaaaatcaa aggcataata atccattacg ctcgatacgc actatggtta ttatgccttt
     8221 aaattatttc tatatataaa tttatggtaa taataaatcg atgcttcaag tatattttac
     8281 tttaatatat ataatcacct atttaattat ttactccaaa tatttaattt ctgttgttta
     8341 gctttatttt gtgcttctat aaatacattt ctatatttgc catttggtga aaaatacttc
     8401 tctctagcaa gtcccttttc cactaattcc ttattgtaca tacgatcttt acttatccat
     8461 acatacgcca atgttctacc atagcgatct tgtttttctt tatcatattc taaataaaca
     8521 tcttgatttg ttaatgtctt cttactataa tttgatgctt ctttgccaaa tggttgtaca
     8581 ggcgtattcg gtttcaccgt ttctggcgta tcaaccccta taagcctaac tttaatttct
     8641 ttaccatttt gatttgcaat aaatgtatca ccatccacaa ctctttgaac atgtacttta
     8701 tctttaccat ttaaatcttg tacagtttca tgattcgtcc cctttttgaa aggacccgta
     8761 tgattcataa attgaaatgc taatacacct acaataatga tggctaccac aatcatagca
     8821 agcgatttat ttgacttcat attgccacct acttctcttt tttcattccg attaatcata
     8881 taaaagttaa gaaatatcgt caatagccat atctacatat ttcaatattt tggaaagcta
     8941 tagcatgata taatgttatt gttaaggagt gaaaataaat gaacatttca aatgaaaata
     9001 cagtcacatt aattgcagta attatcgcaa ttatcattgg tattttccta caaatatttt
     9061 tcaaattacc attaatagtg acggcagtac tatctatttt attgggtatc tttgtaggct
     9121 ttatcgttta cttgattgtt tcgttttata ataagcgaaa aaattagtta acaaatctaa
     9181 attgcgagta ccaaaataaa agtattgagt aagattttga ttattgactc actttccaag
     9241 tctcttaaat caaaacactc aatacttttt tgtttgtatt tagatccagc ctttttcatt
     9301 tgcctttttc caagcatcaa aacgattatc tgcaaataat ttatcaatta taacagatgt
     9361 ataatttcta actgttccat ctgtcaaaaa taatttttca cttatttctt tactacttaa
     9421 accattgcca atttccctta atacaatttg ttctttgggc gttaatgggt ttttatctac
     9481 aaaaaatgaa gtcatcaatg tggcgctata ttctttctct ccgttattta ctttattaat
     9541 ggtttccacc aattcttcta tagaacgttc ttttaaaaca tatgcatcca catcattcac
     9601 aactgctttt tcaaagtatc ccggtctttt aaaagttgtt acaataatca ctttaatatt
     9661 caaatgcttt tttctaattt ccgctaaaac ttcaagtcca gtcatgcctg gcatttctat
     9721 atctaaaaaa acaacgttag gattatattc ttcaataagt ttcattgcat cgagaccatt
     9781 atcagtatct gctaaaattt caaaatcacc atgtagttta attaattgaa ccattgcctg
     9841 tcgtaacata ttttgatctt ctgcaataat taaagatgtc atgctattcc tcctgttgag
     9901 atttcaatga ttattttagt tccatttgtt gagtcgactg ctaaagttcc atttaaatgt
     9961 tgtacacgct gtgaaatact tttcacctca caatcactat cgataccttt gccatcatct
    10021 tcaatcataa gcagtaattt atgattgttt acagttttta atttaccatg aacttttgaa
    10081 gcatttgcat gtttaataac attatttatc gcttcacgcg taatcataac taacatagat
    10141 tgtttagtag gacttaatac ttgcgctaat tctttatttt caaatgtaaa atcaatatca
    10201 gcatctttta aaactttacg tatactatca atctcttcaa taaatgatgg taattttaca
    10261 tcatcaataa tttctcgaac tttgttcaat gattcacgag ataatttatt aattgctaat
    10321 aattcagctt ttactttttc tacatcagca tctattagtt tataagctaa ttctgatttt
    10381 aatgataaac ttgcaaacac atgccctaac gtatcatgta agtcttgacc aattctatgt
    10441 cgctcttgtt cagcaattaa tgtattaata tgtctatttt tttctgctat ttctttttga
    10501 tattcacggt ttttcatttt tttaaaatta tctagcatta ttaacgatat aacgacataa
    10561 taaaccatca ttgcaacaaa atagttgtta tacaataagt acgttattat tgtacatata
    10621 atcattgtta gtatgaaaag attagttgcc gtttttttaa cattattttt aaaagtaaat
    10681 ggaacggcaa aagcactata aaagaaaaac aaacttagca ttggatgtac actgaaaaca
    10741 aaataacaaa taataaaata atgaataatc aacaaaatgt aaaacattaa tgtattaagt
    10801 ctattattta ctataactaa tataagatac gacaacgaaa agacaatgac tgatataata
    10861 tacaaccatt tgggaccata tacttcatta aaaaatatac cggcaatagg aaaaatcaga
    10921 taaactatag acgatatttc agcaattgaa gtatctttta aaaatttcat attttattcc
    10981 gcccttttaa aatgact
//
LOCUS       DANRDI010000089        10901 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.089, whole genome shotgun sequence.
ACCESSION   DANRDI010000089 DANRDI010000000
VERSION     DANRDI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.089"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            241..1083
                     /locus_tag="PZ377_001759"
     CDS             241..1083
                     /locus_tag="PZ377_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDM6358630.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(1208..1519)
                     /locus_tag="PZ377_001760"
     CDS             complement(1208..1519)
                     /locus_tag="PZ377_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDM6358631.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            1606..2682
                     /locus_tag="PZ377_001761"
     CDS             1606..2682
                     /locus_tag="PZ377_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDM6358632.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            2747..3058
                     /locus_tag="PZ377_001762"
     CDS             2747..3058
                     /locus_tag="PZ377_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDM6358633.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            3158..4015
                     /locus_tag="PZ377_001763"
     CDS             3158..4015
                     /locus_tag="PZ377_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDM6358634.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            4044..4640
                     /locus_tag="PZ377_001764"
     CDS             4044..4640
                     /locus_tag="PZ377_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDM6358635.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            4661..8485
                     /locus_tag="PZ377_001765"
     CDS             4661..8485
                     /locus_tag="PZ377_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDM6358636.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            8509..9822
                     /locus_tag="PZ377_001766"
     CDS             8509..9822
                     /locus_tag="PZ377_001766"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDM6358637.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            9896..10387
                     /locus_tag="PZ377_001767"
     CDS             9896..10387
                     /locus_tag="PZ377_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDM6358638.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSTNYKANNVATDANHSYTSR
                     VDK"
     gene            10461..>10901
                     /locus_tag="PZ377_001768"
     CDS             10461..>10901
                     /locus_tag="PZ377_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358639.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKE"
BASE COUNT         4178 a         1480 c         2038 g         3205 t
ORIGIN      
        1 atacctttga tttaaaagaa taaagcttgc attttacttg taaattttaa ttattaattt
       61 aaaaatacag gccgatggtt atgatgcatt tacatataat atgattgcat taatctttat
      121 aagtagttag aagtgtatta gtgtggggcg tttcgttttt gttcgcattt atgaatagtg
      181 atatgatgta ataaacacat gctaattgta ataacgaata cagaaagaat aggtgatgtt
      241 atgaaaatcg gggatatatc tattcattat ctaaatggtg gcaatacaaa aatggatggc
      301 ggtgcaatgt ttggtgttgt tccgaagccg ttgtggtcaa agcaatacaa tgcaaatgaa
      361 cgaaatcaaa tcaatttacc gacacatcca attttgattc aaacggctca atataatttg
      421 attatagatg caggtattgg taatggtaaa ttatctgaaa agcaattacg taattttgga
      481 gtagatgaag aaagtcatat aattgctgat ttggcaaatt ataatttaac gccaaaggat
      541 attgattatg tgctaatgac acatatgcat tttgatcatg ctgccggttt gactgatcaa
      601 gcaggacatg caatttttga aaatgcgatt catgttgtgc aacaagatga gtggcatgag
      661 tttattgcac ctaatataag aagtaaatca acttactggg ataagaataa aggcgattat
      721 agtaacaagt tgattttatt cgaaaaacat tttgaaccgg ttctgggtat caagatgcaa
      781 catagtggag gtcatagctt tggccacacg attattacga ttgaaagtca aggagataaa
      841 gcagttcata tgggtgatat attcccaact actgcacata aaaatcctct atgggtaacg
      901 gcatatgatg attatcctat gcaatcgatt cgtgaaaaag aacgcatgat accatatttt
      961 attcagcaac aatattggtt cttgttttat catgatgaaa actactttgc tgtaaaatac
     1021 agcgataatg gtgaaaacat agatgcatat attttacgtg aaacattagt tgataataac
     1081 taaaataaag atgtattact aaacaaattt tcaaaaataa aaaattgagc cacatccaat
     1141 cttactaatt agggtgtggc tcatttttaa gttttacgat ccaaatcaaa tatggataaa
     1201 attcgtatta acgctctaca atgttaatga cttcaccagt atatgcatct gcataaaaat
     1261 cataatgaat attttgacca tttttaatag ttgtaattcc accttgataa actaaacggt
     1321 atttatcagt ttcaggatga acgaatggtt cataaacgat atatgagcct ttgacattca
     1381 taaaatatga ttttacttca ttaagtactt taacgggatt atagcgttta cgattaataa
     1441 attggatgat tgaaatggta gaaataatta caatggctac tgcaattatt gttggaatta
     1501 tatatttcag tttagtcatc atttatgctc ccttgatttc aaattcatat cattagttta
     1561 ccatattgaa gatgatataa taacttataa ggagtgagaa cttttatgaa cataaataaa
     1621 aaagtaacat tacaacgaat tcaaacttta actgagcttc atggggcacc aggttttgaa
     1681 gaagaagtaa aaaattatat gactcagcaa atggcgccgt acgtagatga atttattgaa
     1741 aatcgtatgg gtggattttt tggtgtgaaa aaatctaaaa atccaaatgc aaaacgtgta
     1801 atgattgcag cacatatgga tgaaatcgga tttatgatta caaatatcac taaaaatgga
     1861 atgattcaat tcacaaattt aggtggtgtt gcaaatgata tttggcaagg acaacgctta
     1921 gtaattaaaa atagaaatgg cgataaaatt atcggtgttg tttctaatat acctaaacat
     1981 tttcgtactg gtagtgaagg tgcaccggaa attaaagatt taacattaga tataggtgct
     2041 caaaatgaag atgaggtgcg tgagcgcgga atagatatag gagatacaat tgtacctcac
     2101 acgccattca cacagttatc tgaacatcga tatagtgcta aagcatggga taatcgttat
     2161 ggttgtgtct tggcaattga aatactagaa ttattaaaag atatagaatt agatgtagac
     2221 ttgtatgttg gcgcaaatgt tcaagaagag gttggattac gaggtgcgaa agcatctgca
     2281 gagatgatag acccagacgt tgcatttgta gttgattgtt cacctgccaa tgacgttaaa
     2341 ggaaaccaac cattatctgg tgaacttggt aaagggacgt taattcgcat aaaagacggt
     2401 acaatgattt taaagcctgt atttagagac tatttattaa agttagtaga agcacatgac
     2461 attgaacatc aatactatat gtcaccaggt ggaacagatg gtggagaaat tcataaagct
     2521 aatattggta ttccgactgc agttattggt gtatgtgcac gatatattca tagtacagac
     2581 tcagtatttg atataagaga ctattttgca gctagatctt tactttcaga agccatttgt
     2641 aatttagata ataatcaaat agaaacatta caatataaat aatcgggtaa taacaactat
     2701 tatctctaaa tagttatata taatcattaa ttaaggagac ataaaaatga aacaacttga
     2761 atcagaacaa caatttgaat ctttaaaaca aggtgctaca gtatttgaat tcactgcagg
     2821 ctggtgtcca gattgtagag tgatagaacc agatttaccg gaattagaag cgagatatcc
     2881 tatgtttgac ttcgtatcag tagaccgtga taaatttatg gatatttgta ttgaaaatgg
     2941 tattatgggt attccaagtt ttctagtata taaaaatgga gaactgcttg gaagttatat
     3001 tggaaaagaa cgaaaatcaa ttgaacagat agatgcattt ttagctcaat acgtgtaatt
     3061 tagactagag aaaaacgggg taatactcgt ttttctctgt tactatgtgt tgatttattg
     3121 taaactatta taaggtgcga aattaggagt gttacatatg aatacctttc aaatgagaga
     3181 taaattaaag gaacgtttaa gccatttaga cgttgatttt aaatttaatc gtgaagaaga
     3241 aactttgcgt atttatcgaa cagataataa caaaggtatc acgattaaac ttaacgctat
     3301 agtcgcaaaa tatgaagata aaaaagaaaa aattgtagat gaaattgttt attacgttga
     3361 tgaagctatt gcacaaatgg cagataaaac ccttgagagt atatcatcta gtcaaattat
     3421 gcctgtcatt agagcgacta gctttgataa aaaaactaaa caaggtgttc cttttatcta
     3481 tgatgagcat actgcagaaa cagcagttta ttatgcagtc gatttaggga aatcatatcg
     3541 tctaattgac gaaagcatgt tagaggattt gaagttaact gaacaacaaa taagagaaat
     3601 gtctctgttt aatgttagaa aattgtcaaa ttcatatacg actgatgaag taaaaggtaa
     3661 tattttttat tttattaact caaatgacgg gtatgatgca agtaggatac taaatactgc
     3721 atttttaaat gaaattgagg cacaatgtca aggcgaaatg ctcgtagcag tgccacacca
     3781 agatgtgtta attattgcag atatacgcaa taaaacagga tatgatgtga tggcacattt
     3841 aacaatggaa tttttcacta aaggtctagt tccaattaca tcattatcct ttggatataa
     3901 acagggtcat cttgaaccga tatttatttt aggtaaaaat aataaacaaa aaagagatcc
     3961 aaacgtgatt cagcgtttag aagcaaatcg tcgtaaattt aataaagata aatagaaata
     4021 attggataag gagttttgtc ataatgaatt tattttacaa tcctaaatat gtaggagatg
     4081 tcgcattttt acaaattgaa ccagttgaag gtgaattaaa ctacaataaa aaaggtaatg
     4141 ttgttgaaat tactaatgaa ggtaatgttg taggttataa tatttttgaa atttcaaaag
     4201 atataacaat tgaagaaaaa ggtcatatta aattaactga tgaacttgta aatgtattcc
     4261 aaaagcgtat ttcagaagct ggttttgatt ataaattaaa tgctgatcta tcaccgaaat
     4321 ttgtagttgg ctacgttgaa actaaagaca aacatcctga tgcagataaa ttaagtgtac
     4381 taaatgtaaa cgttggaaat gacacattac aaattgtatg tggcgcgcct aacgttgaag
     4441 ctggacagaa agttgttgtt gctaaagtag gtgcagtgat gcctagcggt atggtaatta
     4501 aagatgctga attacgtggt gttgcctcaa gcggtatgat ttgttcaatg aaagaattga
     4561 atttacctaa tgcacctgaa gaaaaaggta ttatggtatt aaatgacagc tatgaaattg
     4621 gacaagcatt ttttgaataa ttaaggaagg tagtgaaaat atgagctggt ttgataaatt
     4681 attcggcgaa gataatgatt caaatgatga cttgattcat agaaagaaaa aaagacgtca
     4741 agaatcacaa aatatagata acgatcatga ctcattactg cctcaaaata atgatattta
     4801 tagtcgtccg aggggaaaat tccgttttcc tatgagcgta gcttatgaaa atgaaaatgt
     4861 tgaacaatct gcagatacta tttcagatga aaaagaacaa taccatcgag actatcgcaa
     4921 acaaagccac gattctcgtt cacaaaaacg acatcgccgt agaagaaatc aaacaactga
     4981 agaacaaaat tatagtgaaa aacgtgggaa ttctaaaata tcacagcaaa gtataaaata
     5041 taaagatcat tcacattacc atacgaataa gccaggtaca tatgtttctg caattaatgg
     5101 tattgagaag gaaacgcaca agccaaaaac acataatatg tattctaata atacaaatca
     5161 tcgtgctaaa gattcaactc cagattatca caaagaaagt ttcaagactt cagaggtacc
     5221 gtcagctatt tttggcacaa tgaaacctaa aaagttagaa aatggtcgta tccctgtaag
     5281 taaaccttca gaaaaagttg agtcagataa acaaaaatat gataaatatg tagctaagac
     5341 gcaaacgtct caaaataaac aattagaaca agaaaaacaa aatgatagtg ttgtcaaaca
     5401 aggaactgca tctaaatcat ctgatgaaaa tgtatcatca acaacaaaat caatgcctaa
     5461 ttattcaaaa gttgataata ctatcaaaat tgaaaatatt tatgcttcac aaattgttga
     5521 agaaattaga cgtgaacgag aacgtaaagt gcttcaaaag cgtcgattta aaaaagcgtt
     5581 gcaacaaaag cgtgaagaac ataaaaacga agagcaagat gcaatacaac gtgcaattga
     5641 tgaaatgtat gctaaacaag cggaacgcta tgttggtgat agttcattaa atgatgatag
     5701 tgacttaaca gataatagta cagatgctag tcagcttcat acaaatggca tagagaatga
     5761 aactgtatca aatgatgaaa ataaacaagc gtcaatacaa aatgaagaca ctaatgacac
     5821 tcatgtagat gaaagtccat acaattatga ggaagttagt ttgaatcaag tatcgacaac
     5881 aaaacaattg tcagatgatg aagttacggt ttcgaatgta acgtctcaac atcaatcagc
     5941 actacaacat aacgttgaag taaatgataa agatgaacta aaaaatcaat ccagattaat
     6001 tgctgattca gaagaagatg gagcaacgaa taaagaagaa tattcaggaa gtcaaatcga
     6061 tgatgcagaa ttttatgaat taaatgatac agaagtagat gaggatacta cttcaaatat
     6121 cgaagataat accaatagaa acgcgtctga aatgcatgta gacgctccta aaacgcaaga
     6181 gtacgcagta actgaatctc aagtaaataa tatcgataaa acggttgata atgaaattga
     6241 attagcaccg cgtcataaaa aagatgacca aacaaactta agtgtcaact cattgaaaac
     6301 gaatgatgtg aatgataatc atgttgtgga agattcaagc atgaatgaaa tagaaaagaa
     6361 taacgcagaa attacagaaa atgtgcaaaa cgaagcagct gaaagtgaac aaaatgtcga
     6421 agagaaaact attgaaaacg taaatccaaa gaaacagact gaaaaggttt caactttaag
     6481 taaaagacca tttaatgttg tcatgacgcc atctgataaa aagcgtatga tggatcgtaa
     6541 aaagcattca aaagtcaatg tgcctgaatt aaagcctgta caaagtaagc aagctgtgag
     6601 tgaaagaatg cctgcgagtc aagccacacc atcatcaaga tctgattcac aagagtcaaa
     6661 tacaaatgca tataaaacaa ataatatgac atcaaacaat gttgagaaca atcaacttat
     6721 tggtcatgca gaaacagaaa atgattatca aaatgcacaa caatattcag agcagaaacc
     6781 ttctgttgat tcaactcaaa cggaaatatt tgaagaaagt caagatgata atcaattgga
     6841 aaatgagcaa gttgatcaat caacttcgtc ttcagtttca gaagtaagcg acataactga
     6901 agaaagcgaa gaaacaacac atccaaacaa tactagtgga caacaagata atgatgatca
     6961 acaaaaagat ttacagtcat cattttcaaa taaaaatgaa gatacagcta atgaaaatag
     7021 acctcggacg aaccaacaag atgttgcaac aaatcaagct gtacaaacat ctaagccgat
     7081 gattcgtaaa ggcccaaata ttaaattgcc aagtgtttca ttactagaag aaccacaagt
     7141 tattgagtcg gacgaggact ggattacaga taaaaagaaa gaactgaatg acgcattatt
     7201 ttactttaat gtacctgcag aagtacaaga tgtaactgaa ggtccaagtg ttacaagatt
     7261 tgaattatca gttgaaaaag gtgttaaagt ttcaagaatt acggcattac aagatgacat
     7321 taaaatggca ttggcagcga aagatattcg tatagaagcg cctattccag gaactagtcg
     7381 tgttggtatt gaagttccga accaaaatcc aacgacagtc aacttacgtt ctattattga
     7441 atctccaagt tttaaaaatg ctgaatctaa attaacagtt gcgatggggt atagaattaa
     7501 taatgaacca ttacttatgg atattgctaa aacgccacac gcactaattg caggtgcaac
     7561 tggatcaggg aaatcagttt gtatcaatag tattttgatg tctttactat ataaaaatca
     7621 tcctgaggaa ttaagattat tacttatcga tccaaaaatg gttgaattag ctccttataa
     7681 tggtttgcca catttagttg caccggtaat tacagatgtc aaagcagcta cacagagttt
     7741 aaaatgggcc gtagaagaaa tggaacgacg ttataagtta tttgcacatt accatgtacg
     7801 taatataaca gcatttaaca aaaaagcacc atatgatgaa agaatgccaa aaattgtcat
     7861 tgtaattgat gagttggctg atttaatgat gatggctccg caagaagttg aacagtctat
     7921 tgctagaatt gctcaaaaag cgagagcatg tggtattcat atgttagtag ctacgcaaag
     7981 accatctgtc aatgtaatta caggtttaat taaagccaac ataccaacaa gaattgcatt
     8041 tatggtatca tcaagtgtag attcgagaac gatattagac agtggtggag cagaacgctt
     8101 gttaggatat ggcgatatgt tatatcttgg tagcggtatg aataaaccga ttagagttca
     8161 aggtacattt gtttctgatg acgaaattga tgatgttgtt gattttatca aacaacaaag
     8221 agaaccggac tatctatttg aagaaaaaga attgttgaaa aaaacacaaa cacaatcaca
     8281 agatgaatta tttgatgatg tttgtgcatt tatggttaat gaaggacata tttcaacatc
     8341 attaatccaa agacatttcc aaattggcta taatagagca gcaagaatta tcgatcaatt
     8401 agagcaactc ggttatgttt cgagtgctaa tggttcaaaa ccaagggatg tttatgttac
     8461 ggaagcagat ttaaataaag aataattatg agtaaggagt tttatataat gacacactat
     8521 cattttgtcg gaattaaagg ttctggcatg agttcattag cacaaatcat gcatgattta
     8581 ggacatgaag ttcaaggatc ggatattgag aactacgtat ttacagaagt tgctcttaga
     8641 aataagggga taaaaatatt accatttgat gctaataaca taaaagaaga tatggtagtt
     8701 atacaaggta atgcattcgc gagtagccat gaagaaatag tacgtgcaca tcaattgaaa
     8761 ttagatgttg taagttataa tgatttttta ggacagatta ttgatcaata tacttcagta
     8821 gctgtaactg gtgcacatgg taaaacttct acaacaggtt tattatcaca tgttatgaat
     8881 ggtgataaaa agacttcatt tttaattggt gatggcacag gtatgggatt gcctgaaagt
     8941 gattatttcg cttttgaggc atgtgaatat agacgtcact ttttaagtta taaacctgat
     9001 tacgcaatta tgacaaatat tgatttcgat catcctgatt attttaaaga tattaatgat
     9061 gtttttgatg cattccaaga aatggcacat aatgttaaaa aaggtattat tgcttggggt
     9121 gatgatgaac atctacgtaa aattgaagca gatgttccaa tttattatta tggatttaaa
     9181 gattcggatg acatttatgc tcaaaatatt caaattacgg ataaaggtac tgcttttgat
     9241 gtgtatgtgg atggtgagtt ttatgatcac ttcctgtctc cacaatatgg tgaccataca
     9301 gttttaaatg cattagctgt aattgcgatt agttatttag agaagctaga tgttacaaat
     9361 attaaagaag cattagaaac gtttggtggt gttaaacgtc gtttcaatga aactacaatt
     9421 gcaaatcaag ttattgtaga tgattatgca caccatccaa gagaaattag tgctacaatt
     9481 gaaacagcac gaaagaaata tccacataaa gaagttgttg cagtatttca accacacact
     9541 ttctctagaa cacaggcatt tttaaatgaa tttgcagaaa gtttaagtaa agcagatcgt
     9601 gtattcttat gtgaaatttt tggatcaatt agagaaaata ctggcgcatt aacgatacaa
     9661 gatttaattg ataaaattga aggtgcatcg ttaattaatg aagattctat taatgtatta
     9721 gaacaatttg ataatgctgt tattttattt atgggtgcag gtgatattca aaaattacaa
     9781 aatgcatatt tagataaatt aggcatgaaa aatgcgtttt aatatgttta taatagagta
     9841 gtatgggtat ttattattaa tgacattatt acatgttaat taggaggcgt ttttaatgga
     9901 ttggatttta ccaattgctg gaattatcgc tgcgattgca ttcttaattt tatgtatcgg
     9961 tatcgtagct gtattaaatt ctgttaagaa aaacttagat tatgttgcaa aaacacttga
    10021 cggtgtagaa ggtcaagttc aaggtattac tcgtgaaaca acagatttac ttcataaagt
    10081 aaaccgttta actgaggata tccaaggtaa agtagatcgt ttaaactcag ttgtagatgc
    10141 tgttaaaggt atcggtgact cagtacaaac gttaaacagc tctgtagatc gtgtaacaaa
    10201 ttcaattaca cataatattt ctcaaaatga agataaaatc tcacaagttg ttcaatggtc
    10261 aaatgttgca atggaaattg cagacaaatg gcaaaataga cactaccgtc gtggaagtac
    10321 aaattacaaa gctaataatg tagcaactga tgcaaatcat agctatactt ctagagtaga
    10381 taaataatta ataattaaac aaatgcactt aaatgaggtt gttacttatt taagtgcatt
    10441 tgttatagga ggatatacaa atgacgcagc aacaaaatga taaaagaaca ttaaaaaata
    10501 aacacactta tcaaaatgaa ccactaccaa accgtaaaga ttttgttgtt agttttataa
    10561 ctggcgcgct tgttggttca gctttaggtt tgtattttaa aaataaagtt tatcaaaaag
    10621 cagatgattt aaaagtcaaa gaacaagaac tgtcgcaaaa gtttgaagaa agaaaaacgc
    10681 aacttgaaga aacggttgcc tatacaaaag aacgtgttga aggattttta aacaaatcta
    10741 aaaatgagca agcggcatta aaggcacaac aagcagcgat aaaagaagaa gcaagtgcaa
    10801 ataatttaag tgatacatca caagaagcac aagagattca agaagctaaa agagaagcac
    10861 aagcagaagc ggataaaagt gtggctgtat caaataaaga a
//
LOCUS       DANRDI010000090        10596 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.090, whole genome shotgun sequence.
ACCESSION   DANRDI010000090 DANRDI010000000
VERSION     DANRDI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.090"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1082)
                     /locus_tag="PZ377_001769"
     CDS             complement(<1..1082)
                     /locus_tag="PZ377_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDM6358640.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIV"
     gene            complement(1222..2019)
                     /locus_tag="PZ377_001770"
     CDS             complement(1222..2019)
                     /locus_tag="PZ377_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDM6358641.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            2193..2408
                     /locus_tag="PZ377_001771"
     CDS             2193..2408
                     /locus_tag="PZ377_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358642.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            complement(2705..4264)
                     /gene="rny"
                     /locus_tag="PZ377_001772"
     CDS             complement(2705..4264)
                     /gene="rny"
                     /locus_tag="PZ377_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDM6358643.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(4618..5661)
                     /gene="recA"
                     /locus_tag="PZ377_001773"
     CDS             complement(4618..5661)
                     /gene="recA"
                     /locus_tag="PZ377_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDM6358644.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            complement(5826..6977)
                     /locus_tag="PZ377_001774"
     CDS             complement(5826..6977)
                     /locus_tag="PZ377_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HDM6358645.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            complement(7204..7782)
                     /gene="pgsA"
                     /locus_tag="PZ377_001775"
     CDS             complement(7204..7782)
                     /gene="pgsA"
                     /locus_tag="PZ377_001775"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDM6358646.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            complement(7816..8208)
                     /locus_tag="PZ377_001776"
     CDS             complement(7816..8208)
                     /locus_tag="PZ377_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDM6358647.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            complement(8227..9054)
                     /locus_tag="PZ377_001777"
     CDS             complement(8227..9054)
                     /locus_tag="PZ377_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDM6358648.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            complement(9159..9863)
                     /locus_tag="PZ377_001778"
     CDS             complement(9159..9863)
                     /locus_tag="PZ377_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDM6358649.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            complement(9863..>10596)
                     /locus_tag="PZ377_001779"
     CDS             complement(9863..>10596)
                     /locus_tag="PZ377_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDM6358650.1"
                     /translation="DLYLCYETFYHPSNMVLFVVGDVDPEAICRIVKQHEDARNKVNQ
                     PKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYVQRDLEMSLFFE
                     LIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDKLKKLLLDELRD
                     KKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVFDMLDIVENITL
                     DSINETSSLYLNLDQQVDSRLEIKK"
BASE COUNT         3310 a         2051 c         1402 g         3833 t
ORIGIN      
        1 actataacag ggcattgata ttgttctgct aaattaaatg cttccatagt taaataaaat
       61 gcatcttctg catctgttgg tgctacaaca atttttggaa tatcaccatg tgtaccataa
      121 atcatttgca ttaaatctga ctgttcttgt ttcgtaggta atccagtaga aggtccacct
      181 cgttgggtat taataatgac taatggcgtt tcagtcatac cagataatcc aattgcttcc
      241 atcattaaag ataaacctgg accagcacta gccgtaaacg ctctgactcc accataattt
      301 gcaccaatag ccatagttac agcagcaatt tcatcttctg tttgaataac cgctccgttt
      361 actttagata tattggcaat catatattcc ataacttcag acgcaggtgt aataggatat
      421 gccgccataa attgtgaacc tgcagcaatt gcacctaatc caatggcatc gttaccaatc
      481 atatatagat gtggtagtgc atctgttgac tctaattcaa agtccccgta gatttcaggt
      541 aagcgagatt gcattaattg ataaccttcg tttaatgctt ggatattgac ttcaactacc
      601 ttgtcacctt ttttagaaaa catattagta ataagttctt caaatgtatt tgtattcaaa
      661 ttcatcaatg cgctagtagc acctattgca accatgtttt tcattaatgc tgtacctaat
      721 tctttagcgg ttgctgtaaa aggtaattca ataagctgtg catgacatcc ttctggtttc
      781 acaggtttag ccttggcatc agctaaaata atactgtctt ctctcatttc atgatggtta
      841 acatcaattg tttcttggtc aaatgcaatc aaaatatcta aatcatcact aattgcatga
      901 acaggcgtcg tagaaactct aattttatta ttcgtatgtc cacctttgat acgacttgaa
      961 aaatgtctat atccatataa ataatatcct tttctattca tagccgtagc gaagatttcc
     1021 ccagttgatt caataccttc gccttgttga ccgccaactt tccacgataa ttgtggtttc
     1081 atagttatat gcctcctatg gatatatttc atattaaatg ataacaaaaa gaaccaccga
     1141 tttactatgt aaacagtggt actatattaa attcatacta atggacgata gggtccaatt
     1201 cgaatgttta cttacgtaat tttaaaatgt tgaaaatgga tggtcatcat ttatcaatat
     1261 acgttcgatg tgctttgttt taccttcttt gtctaaatca ataacaacac cagataatac
     1321 acttctacct tcatttggaa caacatgtct ttgtggcaaa ctagtgataa aacgctcaat
     1381 tacctctgtt ttatttattc ctaaaatgcc atcataaaaa cctgtcatac caacatccgt
     1441 tatataccct gtcccctttg gtaaaatacg ttcatctgct gtttgaatgt gtgtatgcgt
     1501 tccaacaaca gcgctagctc taccatctaa atgccatccc attgcatact tttcagaagt
     1561 tgtttctgca tgaaaatcaa caaatataaa cggagtttgt tcttgtgctt ccttgactaa
     1621 ttgatctgcc tttttaaaag gatcatcaat atctggcata aacgctcttc cttgcagatt
     1681 aataactgca agtttaatat cattaatttg tataaatctc ataccaattc ccggcgcttc
     1741 atccggaaaa ttcgctggtc ttactagtcg ttttgcttca tctataaaat cataaatttc
     1801 acgttgacca tatgtgtgat tacccatagt catgaaatct acaccatttc ttagtaattg
     1861 tttatatatt ttttcagtca aacctttacc atgtgctgca ttttcagcat ttacaattgt
     1921 aactgttggt ttatactttt gcttcagttg aggtatgtac gtttcaattg cgtctcgtcc
     1981 aattttacct acgatatccc ctataaacat tattctcatg ttttgttcat cctctcataa
     2041 atactagttt aattcatacg ttctaatatt taaagtaaat attaaattct ttgaattatt
     2101 tttcttcaat tatagagaga cattactttg tctgaaaact tcaatatgtt agtattaaga
     2161 catcaaaata aataggagtt gtaaataaat acatgtctat taatattgat ccagaaaaat
     2221 ttgcagaatt agtggttatg tctaacccat caaaattcga agatgcagag gatatagcta
     2281 aagaaagttt aaagctttac attaatgcat atcgtttagc agagagatat tcaacaattg
     2341 caacaaattg ttatgatact gcagaagtta taaaagaatt aaagaaaaca gatttacaat
     2401 taaaataact caacttagaa ataaatccta attaatattg cagaagagaa gatgtgttac
     2461 atcttctctt cttttttatt ggatagaaaa gcaatcatct ttataggttt acataatata
     2521 aatatagtaa ttacgaagcc taaaacattt ttattaaaag caataacttc ttatttaata
     2581 taaggtccgt cttactttca aataactact gtttaatata gttattaccg ataaagtgct
     2641 gtctaactag attaagacta caacttaaaa aagctccctc actaatttgt gagggagaca
     2701 aaaattattt cgcatattct actgctctag tctctcgaac aactgtcacc ttgatatgac
     2761 caggatattg taattcatct tcaatctgat ttttaatatc tctagccaat cgataagatt
     2821 ttaaatcatc aatttcttca ggagatacaa tcactcggat ttctctacct gcctgaatcg
     2881 caaatgcttt ttctacacca tcataacttt ctgataacgt ttctaaacgt tctaatcgac
     2941 gaatataatt ctctaatgtt tcttttcttg cacctggacg agccgcagac aatgcatctg
     3001 cagcagcaac aaggatagat ataatagatg taggttcaac atcaccatga tgagaatgga
     3061 ttgcattaat aactgtttca ttttcaccat attttttcgc taattctaca ccgatttcaa
     3121 catgactacc ttctacttca tgatcaattg ctttaccaac atcatgtaaa agtccagctc
     3181 gtttcgctaa tgtctcatct tcgcctagct cagcagctaa catactagca agatgcgcaa
     3241 cttcaattga atgtttaagt acattttgac cgtaactcgt acgatagttt aaacgcccta
     3301 caatttttac taagtcagga tgcatattat gtgcgttcac ttcaaatgta gcttgttcac
     3361 ctgcttctct aataatatcg tctacttctt ttctagcttt ttcgaccata tcttcaattc
     3421 tacctggatg aatacgtcca tcagatacta agttaacaag tgctgttcta gcaatttctc
     3481 ttcttattgg atcaaaacca gataatataa ccgcttctgg tgtgtcatca ataattaaat
     3541 caatgccagt taaagtttca agtgtgcgga tgtttcgtcc ttctcgtcca atgattcgac
     3601 ctttcatctc atcattaggt aagttaacta ctgataccgt tgattcactt gtgtgatctg
     3661 ctgctaatct ttgtactgct gtagctaata attcttttgc tgttttatca actttttctt
     3721 tagcttcttt ttctttttct ttaacaagta ctgcaatatc ttgtgacagt tcttcctcta
     3781 ctctttgaag ttgctcatta atagcttctt cttgagtgag accggagatg cgttctaatt
     3841 cttgttcatg cttcattatt aacgtttgaa cactactctc ttttgcatct acttgttgtt
     3901 gtttttcttc aatttttgat tctttttgct ctaaaatctc atctttttta tctaatagat
     3961 cagatttgcg ctctaagttt tcttcttttt gaagaagtcg ggtttcttgt ctttgaagtt
     4021 cgctacgtct ttctcgtagt tctgcttcag tttgttctct taggatttgg ttttcttctt
     4081 ttgcctcaag taatttctct tttttgatat tgtcagcttc tttatgtgct tgatttacaa
     4141 tatcttcggc agtttgtcta gcttgtgatt gcttttgaag caacaaattt cgggcaacaa
     4201 cataccctcc aacaactcct agaatgatcc ccagcaaaat gagtaggagg cttaataaat
     4261 tcacacaaac acctcctttt ctagggtttg ctctttatat gaaattcaat atgactttat
     4321 gaaattatat ataaatcata caaattaatt gtacggttta taggagataa gtgtcaagtt
     4381 atcaatttgc acttttgcac tatattgaat gctatttcta ctattttcta aagttttgaa
     4441 gctcgattat aaaactattg ataatgtaat aataaataaa ataaaaaacc ttatagtctc
     4501 gatttgtagt gtatcccata aagttagata ttattctagc tttatgagat gaatatcact
     4561 ttcaatcaat cctataagga tatttgctaa gtttaactat agatataaat ttgtgtacta
     4621 ttcttcgtca aataatgact ttggtgcatc ttctgtttct tcaacatcac catcagatat
     4681 acctaatttt tctctcaatt tacgatcaat ttcttcttta atttgtggat tttctttcaa
     4741 gtacatttta acattttcct taccttgacc cattcgttcg ccattgtaag aataccatgc
     4801 tcctgattta tcaacgatgt cgttttcaac acctaaatca ataagttcac cctctttaga
     4861 aataccttgt ccatacataa tatcaacttc agctactcta aatggtggtg ccactttatt
     4921 tttaacgact ttaattttag ttctattacc tacaatttct tgtccttgtt taagctgttc
     4981 tgcacgacgt acttctagtc ttactgaact atagaatttt aatgcacgtc cacctggtgt
     5041 agtctctgga ttaccgaaca taacaccaac tttttcacga atttggttga tgaaaatagc
     5101 agttgtattt gatttagaaa tagcacctga aagtttacgt aacgcttgtg acattaaacg
     5161 agcttgtaaa ccaacgtgag tgtctcccat ttctccttca atttcagctt taggtgttaa
     5221 agcagcaact gagtctacaa ctacaatatc aactgcacca cttctaacaa atgcttcggc
     5281 gatttcaaga ccttgttcac catgatccgg ttgcgataaa tataaattat cgatatctac
     5341 gcctaatgct tgagcatatt ctggatctaa agcatgttca gcatcgataa atgctgccac
     5401 cccgccatta ctttgtactt cagcaatagc gtgaagcgct actgttgtct taccagaact
     5461 ttcaggacca taaatttcaa taattcgtcc tttaggatag ccacctacac ctagcgcatt
     5521 atctaatgta actgaaccag ttgatgtagt tgaaactcgg cgacctatat tgtcacccaa
     5581 cttcattacg gcacctttac cgaaagattt ctccatattt ttaattactg tatctaaagc
     5641 tttttgacga tcgttatcca aagcgagacc tcctaattga aattgctatc ttttaattat
     5701 cttaccaata ctatacaagg attcaaaata aaatacaagt gttttgcgaa tatttgttcg
     5761 tataaaatct tgaaatcaaa acgcaaaaac gaacaaacgt gcttatattt ttatcgataa
     5821 tacaattaat ttgttgttct aagccaattt aataatctta tgagcgcata attttgactt
     5881 ctgttcttta ataaatttct agatttagac attttaaatt gctcagtttt aaattcaaag
     5941 ttattatcat agcctatatg aactacacca tcatactcta atagaataat tgctgatgac
     6001 acattaaaca atttattaac aaattgcact gcatctttta attgctgctc aataggttta
     6061 ttaatatcaa caaagttttc attgtgatta atcataccct ttagaacatc gtttaaatcc
     6121 acttctttca atcgatgata taaagcacca ttagtaatac catcatatat tacaaaaggt
     6181 tcatgaattt tctttattac agcttgctca attaatgtgt catctgaacc ataataatat
     6241 tctccaatac gatcaagaat ttcttgttta acaggttgaa tcaatgattg tgcttgttct
     6301 tttgagtcgg catttgcagt caatctaata tatacttcat gacttcccgc caaaggcgca
     6361 atcgtaggat tagtctgttt atcgataaga tctattaata ttgtttctac tttagattca
     6421 cctattcccg caaatcttaa tagttcagaa tgtataattc gattatggtt tataaaatgt
     6481 gacaacaatt catttttcac cattggttgc atttctttcg gtggacctgg taataaaata
     6541 atttgtttgt tttcaaaatt caccatcatt cctggagcca tgccatgatg atttgttaat
     6601 acagttgaac cttcaattac taaagcctgt tgtttattat taggtgtcat ttcttgtcct
     6661 tgttcctcaa aatagctttc aatatatttt aaagaaggct catcaataac taaatcttta
     6721 ccaacaatct gggccactgt atgcttcgtt aagtcatctt tcgtaggacc taagccacct
     6781 gttaaaataa cagtatcata tttttctagc gcatgacgta cacttgattc taaacgtttt
     6841 ttattatctc caataacttt atgttctaat acattttgtc caatttcatt aaatacttta
     6901 gatagaaatt gtccgttggt attagcgatt tgacctagca atagttctga gcctacagca
     6961 ataatggcaa ttgacattta tatccccctt gtatcgttta attcattttt tcatgtattt
     7021 catttaaaaa ttcacatcaa ttttagattc aatgtatcag atgctactac agattttccc
     7081 taaaaaaatg aacaaatatt gttttacaat atatcattat aaacaattgc gtggctaatt
     7141 tctaaaattg ggattaaaaa aactagattc catatgaaaa tgaaatctag tataaacaaa
     7201 tatttatttt tgtttaaaaa catctctacc tttataaaag tattcaatac cagataagat
     7261 agtaaaaata acgccaatgt ataataaaat ttgtcctaat gacaaaccaa tcaatgttgc
     7321 caatggatca cctaataaca accaagtaat tgctaccata gtaactgctg ttttaatttt
     7381 acctaattga ccagctgcac ttacgaatcc ttgttcaatt tgtagtaaac gtaaaccagt
     7441 tacggcaaat tctctggcaa taataatgat tgctactaca gaatttgtta gtcctagttg
     7501 cacaagtaca attaaagcac ttgcaactaa taatttatcc gctaatggat ccaaaaattt
     7561 ccccatattt gtaactaaat tccattttct agctaaataa ccatcaacaa aatcgctaag
     7621 ggaagccaat ataaaaataa aaccactgat taataactca attcttattt catatcctcc
     7681 tagaaatgac acattgccaa atccaaaatc aactaacgca aacaatataa aaactggtat
     7741 taacactact ctaaaaaccg taatctggtt cggaatattc attatacatc ctcatttctc
     7801 actaatttat ttctgttaaa atattaaaac taacatgatc cataacaata aagttattaa
     7861 aactgtaata cccatgatta ctaataattg tattggctct tttgatttac tcttataatc
     7921 taagtcttta ttattattga aaactgtaat tacttcgtcc cattcggctt ggttcgatgg
     7981 aatttcatct tgatgagctt gaattaattg gttaggttca atatttacta cgcttgcata
     8041 ttttctaata aatccttcgc tgtaattttt attcggtagt tgatcgaatt cattattttc
     8101 aatatgcact agcatttcac gtttaattcc agtacgttgc tctaattctg ttaaagtcat
     8161 tcctaacctt tcacgtctac cttttagcgc ttcaccgacc gttttcaatt catagcctcc
     8221 ttacacttac tcgaagaaac caaaaccacc accaaacgga tctccaaaat ctaaattatt
     8281 cttactttgg actgtctgtt tcttcatttc ttcgtaatgt atttcttgat tttcattttc
     8341 tctcaattcg ataatatagt caaaatcttc cattgtacaa gtacttgttt caacaaataa
     8401 atctggatgt tcaacgactt tgattgatgg taacgtcata acttcgttaa caagctcttg
     8461 atgttttgga ttagattctc tggcagtaac tgcaccatca ataatgtata catgattggc
     8521 atcatattcc cctttaatta gagagctacg tacagtttgt tttattaaag tagaactaat
     8581 aaataaccat ctcttatgtg cacaaacact tcccgcaaca attgattcag ttttaccaac
     8641 ccgtggcata cctctaatgc caatcaactt atgaccttct tctttgaaca attcagctaa
     8701 aaagtctact aacaagccta aatcttcacg ctcaaatcga aaggttttct tatcttttgc
     8761 atcttgctca atatatcttc catgtcttac tgcaagacgg tctcttaatt ctggtttttt
     8821 aagctttgtt atttcaattt catttatacc acgagctatt tgctcaaaac gttcaacttt
     8881 ttcaagattg tctgttttaa ttaaaaggcc tcgtttacct tgatcaacac cattaattgt
     8941 aacaatactt atacctaaca tacctaataa actagataca tcacctaaaa gacccggtct
     9001 attaatttca atttcgtatt ctaggtacca ttcttttttc tctgcaactg tcatggtgaa
     9061 cctcacccct tgtttaatta acaaattatg attcattgta atattcatat tataacatca
     9121 atcataaaaa catttgctca taaaaatagt tctatatttt aaatatacca agcaccatta
     9181 actttctgta tagttccagt aacacttcta gcatttgggt gatataaata ggcgcatgta
     9241 tgagcaacct cactcggtaa aaccaatcgc tgttgtggta attcagttat cattgcttga
     9301 agttcatctt cttgccactc acttgccata ttaccggcta caaatccagg tgcgatagca
     9361 tttactgtca ctgatgttag tgcaagctct tgactaagcg ccttaacgaa acctaattgt
     9421 gcacttttca tcgccgaata aatagtttcc atactagctc ctgtctcacc ccaaattgat
     9481 gaaattacaa taattcttcc attggcactt tgacgtaaaa catcaacgaa atatctacat
     9541 aatcgaatta attgcaagac atttaactga taacatgcat caatatcatg gtctttcata
     9601 tcttgtaaaa caccatataa agactgacca cttgcatata ttaaacagtc taatgattta
     9661 atgtcaccaa atgttttgtc aatatcaatt gtttgagata aatccgcttg tataaaacga
     9721 actttatcat cattaaattt gctagttaat tcatttatat cagtacgata atattgcaca
     9781 taaacttcaa atccatcagt taataattgt ttgactatct cagaaccaat tgaaccagaa
     9841 ccacctaata ctaatgcttt cattactttt taatctccaa acgactatcg acttgctgat
     9901 ctaaatttaa atataacgat gacgtttcgt taatactatc taacgtgata ttttcaacaa
     9961 tgtctaacat gtcaaacacg ctaacacctt caaaatacaa tttagtatat tgattagcaa
    10021 tatattcagg tgagtttaaa cttgatatga attcacctat aaattgcttt ttcaaaagtt
    10081 caaatgcttc tgcatcttgg aaattgcctt ttttatcacg caactcatct aataataatt
    10141 tttttaattt atctggttct tcagtagcac ttgtcacgat tgaaaaacta tacgtcggct
    10201 ctagtacaaa ttgataacca aatgtatcat cgataagtcc ttcgtttaat aaattctgat
    10261 aaaaatctgt ttcttcccca aaaattaact caaagaataa tgacatttct aaatcacgtt
    10321 gtacatattt ttgaggcgct tcttgtaatg gtttattttt aaaaccaagc attagtcttg
    10381 gtgattgaat tttcatagat tcagtaacaa atgcttcttt aacatcctcc ggttcatcaa
    10441 caagtcctcg ttcgattttg ggttggttaa ctttattacg agcatcctcg tgttgtttta
    10501 ctattcgaca tattgcttca ggatccacat cgccaacaac aaataaaacc atatttgatg
    10561 gatgataaaa cgtttcataa catagataca aatcat
//
LOCUS       DANRDI010000091        10506 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.091, whole genome shotgun sequence.
ACCESSION   DANRDI010000091 DANRDI010000000
VERSION     DANRDI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.091"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..443
                     /locus_tag="PZ377_001780"
     CDS             <1..443
                     /locus_tag="PZ377_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDM6358651.1"
                     /translation="TRELVYHNGAVAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKL
                     EDDEDRVEAAKRELEEETGYIAKELTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLD
                     EDEFVEVIKVPIENVKSMLMNKEIEDAKTIIALQHLLLNYNHSK"
     gene            548..997
                     /locus_tag="PZ377_001781"
     CDS             548..997
                     /locus_tag="PZ377_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDM6358652.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            1046..1933
                     /gene="xerD"
                     /locus_tag="PZ377_001782"
     CDS             1046..1933
                     /gene="xerD"
                     /locus_tag="PZ377_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDM6358653.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(2011..2517)
                     /locus_tag="PZ377_001783"
     CDS             complement(2011..2517)
                     /locus_tag="PZ377_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDM6358654.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            2609..3340
                     /locus_tag="PZ377_001784"
     CDS             2609..3340
                     /locus_tag="PZ377_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDM6358655.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            3333..3875
                     /gene="scpB"
                     /locus_tag="PZ377_001785"
     CDS             3333..3875
                     /gene="scpB"
                     /locus_tag="PZ377_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDM6358656.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            3868..4605
                     /locus_tag="PZ377_001786"
     CDS             3868..4605
                     /locus_tag="PZ377_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDM6358657.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            4738..5463
                     /gene="srrA"
                     /locus_tag="PZ377_001787"
     CDS             4738..5463
                     /gene="srrA"
                     /locus_tag="PZ377_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDM6358658.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            5444..7195
                     /gene="srrB"
                     /locus_tag="PZ377_001788"
     CDS             5444..7195
                     /gene="srrB"
                     /locus_tag="PZ377_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDM6358659.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            7440..8369
                     /locus_tag="PZ377_001789"
     CDS             7440..8369
                     /locus_tag="PZ377_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDM6358660.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDK
                     EFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKS
                     TITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
     gene            8362..10388
                     /locus_tag="PZ377_001790"
                     /pseudo
     CDS             8362..10388
                     /locus_tag="PZ377_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4014 a         1354 c         1963 g         3175 t
ORIGIN      
        1 caacaagaga attagtttat cataatggtg cagttgctgt ttgtgcagtg acacctaaaa
       61 aagaagtcgt attagtgaaa cagtatcgta aaccagtaga aaaaccattg ctagaaattc
      121 cagctggtaa attagaagat gatgaagata gagtcgaagc ggcaaaacgt gaattagaag
      181 aagaaacagg atatattgcc aaagagttaa cacatgttgt agatatgtat ggttcaccag
      241 gtttttgcga tgaacaatta tcaatatatt ttacggataa tttagaagaa ggtacagttc
      301 acttagatga agatgaattt gtcgaagtca ttaaagttcc gattgagaat gttaaatcga
      361 tgctaatgaa taaagaaata gaagatgcga agactatcat tgcattgcaa cacctattat
      421 taaattataa tcattctaaa taacaaatga gaaaagcttg cattttattg agaaaactgt
      481 tagttttaat tgtgaagttt gaaataattt gtaatgattt taattattag taggggagtg
      541 gacatcgttg gaagaacgat taaatcgcgt taagcaacaa ttacaacaat catcatataa
      601 gctaacgcca caacgcgaag ctactgttag agttctaatt gaaaatgaaa aagatcatct
      661 aagtgctgaa gacgtatatc tgaaagtaaa agataaagcg cctgaaattg gcttggcgac
      721 agtatacaga acgttagagt tgttagctga actaaaagtt gtcgacaaaa ttaactttgg
      781 tgatggcgtc gctcgttttg atttaagaaa agaaggcgca aaacatttcc accatcattt
      841 agtatgtatg gaatgtggtc gtgtagatga aatcgatgaa gatttgttac cagaagttga
      901 aaatcgagtt gaaaatgagt tcaattttaa aattttagat catcgtttaa ctttccatgg
      961 tgtgtgtgaa acgtgccaag ctaaaggtaa aggatagtaa attgcgtagg ttaaattaac
     1021 cttcgctttt tttagaggtg tggttatgga aacaattatt gaagaatatt tgcgttttat
     1081 acaaattgaa aaaggactaa gttctaacac aattggtgct tatagacgtg atttgaaaaa
     1141 gtatcaagat tatatgactg aacatcatat ctcgcatatt gattttatag atcgacaatt
     1201 aattcaagag tgtttggggc atttaataga ccaagggcaa tctgctaaat ctattgcgcg
     1261 atttatttca acaatccgta gttttcatca atttgctata agagaaaaat atgcggcgaa
     1321 agatccaacg gtattattag attcaccaaa atatgacaaa aaattgcctg acgttttaaa
     1381 tgttgacgaa gtgttggctt tattagaaac gccagattta aataaaatta atggatatcg
     1441 tgatcgtacg atgttagaac ttctgtacgc aacgggaatg cgtgtatctg aattgataca
     1501 tttagagtta gaaaacgtga acttaataat gggatttgta cgcgtatttg gtaaaggcga
     1561 taaagaaaga attgtaccat taggcgacgc agtcattgag tacttaacta cttatattga
     1621 aacgattaga ccgcaacttt taaaaaagac tgttactgaa gtcttatttt taaatatgca
     1681 tggtaaacct ttatcacgac aagcaatatg gaaaatgatt aaacaaaatg gtgtaaaggc
     1741 aaacattaaa aagacgttaa cgccacatac gttacgccac tcttttgcga cacatttatt
     1801 ggaaaatggc gcagatttaa gagcagtgca agagatgtta ggtcactctg acatatctac
     1861 tacccaactc tatacacatg tttcgaaatc tcaaattaga aaaatgtata accaatttca
     1921 tcctagagca taaagtgaac aataactcaa aagtcacaat acacatgact aaaaatgtct
     1981 gtgctattgt ggctttttta aattggttga ttaattacgt ctatgttttc ttaattgaat
     2041 cgcttcttct tttgctgcaa tcacttctga acgatcacgg cgcatgtgat ggtctacaat
     2101 aaaaggatct gttgctgttt cctgattata atcatagtct ggatagttgg ccttgatgat
     2161 gcgttcaaag actggagtta ttggtaatat aacagatgaa aaaggctttg ctgcattcaa
     2221 ttttgcaatc tgttgctcaa ttaacaactg ataatcattt aaattaaggt ataacgcatc
     2281 tctatcttta gcattttgta ttatttcttt agatttatta aaagacttat aggcgccttt
     2341 taaattattg cggcgataat ggtaacaagc agttgcaaac aagattaaac taacaactgc
     2401 atcttgctta ctgtagttat tttcagcttt ccatgcatct tctaaaatgt catgacatag
     2461 gaaataatgt tgcttagtat gaaattgata atagaaattt atcagtgcct gttgcatttt
     2521 gttatcaccc caatttaaaa gtaagttatt ttcatgctat aatattttag agaattatgc
     2581 acatatgacg caatacgagg tagatattat gtatgaagtt aaattagatg ctttcaatgg
     2641 accattagat ttattgctgc atcttatcca aaaatttgaa atagatattt atgatattcc
     2701 tatgcaagca ttaacagagc agtatatgca gtacgttcat gcaatgaaac agcttgaaat
     2761 taatattgca agtgaatacc tagtattagc gtcagaactc ttaatgatta aaagtaagat
     2821 gctattacca caatcaacat cagatatgga tgttgatgat gacccacggg aagatttagt
     2881 tgggcgttta atagaatatc aaaattataa agaatatact gctattttaa atgacatgaa
     2941 agaagaaaga gatttttatt ttacaaaaag accgacagat ttatctcatt tggaaacaga
     3001 tgaatcttgg gatccaaatc atacgattga tttaactgaa ttaattgtag cttatcaaag
     3061 agttaaaaat agagttgagt taaatacacc taaatctgtt gaaatccgaa aagagacatt
     3121 taccattcaa caagctacag aacaagtgac atcgagattg aaagataaag atcattttaa
     3181 cttctttagt ctgtttacgt tttctgagcc aattgaacaa gtagtcactc actttttagc
     3241 tattttagag atgtcaaaag caggaataat taatattgag cagcaacgta attttgaaga
     3301 tattaacatt attagaggag tgaactacca ttttggataa tcatggtata ttagagtcgc
     3361 ttttatttac agctggcgat gaaggtttag atgaaaaaca actattagaa atattagata
     3421 tgtcgaaaga ccaactcgtt gaattaattg aaaattattc atcacatgga ttaatgatac
     3481 aacgatttgg aatgacgtat gttttaacga ctaaaaaaga agcggcaacg tatattgaac
     3541 aattaattga acaaaagtca caaatgaaat tatcacaagc agcaatggaa gtactatcaa
     3601 ttattgctta taaccagcca ttatcaagaa gtgatattga attaattcgt agtatcaatt
     3661 cagatggtgc agttaagaca ttgattgcca aaggactagt tgaggctaaa gtggttaatg
     3721 aacagcgtag ccaacagtta attactactg atttattttt aaatgtattt ggtatttcca
     3781 atatagaaga tttgccgaca actgaagaag acgatgaaga aatggatgct tttttcagta
     3841 atctagtcaa tcaaaaagga gaaaataatg actaaagaat tagaacgatt acaaaaacgt
     3901 atagctaata gcggttatac ttcaagacgt aaggcagaaa ctttaatttc tgaaggaaaa
     3961 gtgaaagtga atggtacgac tgtcactgaa ttaggaacaa aagtgaagcc atcagatacg
     4021 attgaagttg aaggtattaa aatagaacta gaagacaaaa tttacatttt attccataaa
     4081 ccaactcaag tgataacaag tgtatctgat gatagaggac gtacagtggt tacagattac
     4141 tttaaagata tagaagcacg tatttatcct gttggtcgtt tagactatga tacttctgga
     4201 ttgttattac tcacaaatga tggtgaattt actaatttaa tgacacatcc aagatatcaa
     4261 attaagaaaa aatatgttgc gaaattaaaa ggttatttaa tgagagaaga agtgaaagcg
     4321 ctagaaaaag gtattgaatt agaagatggt atgacgcaac cggctgaagt gaaagttaag
     4381 aaacaagata aagataaaaa cacaacattg gttgaaatta caattacaga aggtcgtaat
     4441 cgccaagtca gaagaatgtt cgaacatttt ggtcatcaag tgacgaaact gtctcgtatt
     4501 gaatatgggc ctttaaatgt tgtcggtttg aatgctggtg aaggacgtgt attgacgcct
     4561 catgaagtaa aagtgatgcg tcatttagca gaacatggga aataattaaa taaaatatgt
     4621 atttatcaca aagtttgaga attttttcac aaaattttag gtttttaata actgtatgcg
     4681 ctttcctgtg atataatact tttgtaaaga aaagcatgtg tgggaggtat gacctgtatg
     4741 tcgaacgaaa tacttatcgt agatgatgag gatagaatca gaagattact taaaatgtat
     4801 ttagaaagag aatcttttga aatccatgaa gcaagtaatg gccaagaggc ttatgaactt
     4861 gcaatggaga ataattatgc ttgcatacta ctagatttaa tgttgcctga aatggatggt
     4921 atccaggtgg caactaaatt gcgtgaacat aaacaaacac cgattattat gttgactgct
     4981 aaaggtgaag aaacaaaccg tgttgaaggt tttgaatctg gtgcagatga ttatatcgtc
     5041 aaaccatttt caccaagaga agtagtctta agagttaaag cacttctaag aagaacgcaa
     5101 tctacaactg tagaacaaag cgaacctcac gcacgtgatg tgattgaatt taaacattta
     5161 gaaatagata atgatgcaca tcgcgtactt gctgataatc aagaagttaa tttgactcct
     5221 aaagagtacg aattattaat atatttagct aaaacaccaa ataaagtatt tgaccgtgaa
     5281 caattattaa aagaagtttg gcattatgaa ttctatggtg atttaagaac agttgatact
     5341 catgttaaac gacttagaga aaagttaaat cgtgtgtcta gtgaagctgc gcatatgatt
     5401 caaacagtct ggggcgttgg gtataaattt gaggttaaat ctaatgatga gccggctaaa
     5461 tagtgtcgta attaaactgt ggttaactat tattttaata gtgacgacag ttttaatttt
     5521 attaagtatt gctttaatta cctttatgca atactatttc acacaagaaa ccgaaaatgc
     5581 cataagagaa gatgctagac gtataagttc actggtcgaa caatcacata ataaagaaga
     5641 agcaataaaa tatagtcaaa cattaattga aaatcctggt gggttgatga ttataaataa
     5701 taaacatcgt caatcaacgg cttcactttc taatattaaa aagcaaatgt tgaatgaagt
     5761 agtcaacaac gaccattttg acgatgtgtt tgataaaggt aaatctgtta ctcgaaatgt
     5821 aacgattaaa gaaaagggct catctcaaac atatattttg ttaggctatc caacaaaagc
     5881 acagaagaat agtcatagca aatatagtgg agtctttata tataaagact tgaaatcaat
     5941 cgaagataca aataatgcta ttacgattat caccataatt acggctgtta ttttcttaac
     6001 aattacaaca gtctttgcgt ttttcttatc gtcaagaatt acaaaacctt taagacgttt
     6061 aagagaccaa gctacacgtg tatctgaagg ggattactct tataaacctt ctgtcacaac
     6121 gaaagatgaa attggtcaat tatcgcaggc atttaatcag atgagtacag aaatcgaaga
     6181 gcatgtcgac gcattatcca catctaaaaa tattagagac agcttaatta actctatggt
     6241 agaaggtgtc ctaggtatta atgagagtcg acaaattatc ttatctaata agatggcgaa
     6301 tgatattatg gacaatattg atgaagatgc taaagctttc ttattaagac aaatagaaga
     6361 tacttttaaa tcaaaacaaa ctgaaatgcg cgatttagaa atgaatgcac gattctttgt
     6421 tgtgaccaca agctatatcg acaagattga acagggaggt aaaagtggtg ttgttgtgac
     6481 agttcgtgat atgactaatg agcacaatct agatcaaatg aagaaagatt tcattgctaa
     6541 tgtatcacat gaattacgta caccgatatc attacttcaa ggttatactg aatcaattgt
     6601 agatggtatt gttacagaac cggatgaaat aaaagaatcg cttgccattg tccttgatga
     6661 atcgaaacgt ttaaatcgtt tagttaatga attgttaaat gtcgcacgca tggatgctga
     6721 agggttatcc gtaaataaag aagttcagcc tattgcagcg ttactagata agatgaaaat
     6781 taagtatcgc caacaagctg atgatttagg tctaaatatg acttttaatt attgtaagaa
     6841 gcgtgtttgg agttatgata tggatcgcat ggaccaagta ctaacgaact taattgataa
     6901 tgcatcacgt tatacgaaac ctggagatga aattgcaatt acttgtgatg aaaatgaaag
     6961 cgaagatatt ttatacatta aagatacagg tacaggcatt gcaccagaac atttacaaca
     7021 agtatttgat cgtttttata aagttgatgc agcgagaacg cgaggtaaac aaggtaccgg
     7081 tttaggtttg ttcatttgta aaatgattat cgaagagcat ggtggttcca tagatgttaa
     7141 aagcgaatta gggaaaggca caacatttat tattaaacta ccaaaaccag aataaaattg
     7201 aatatagtta tttcagaacg catgttattg attagagact ctaatttata gcatgcgttt
     7261 tttgattgat gtggggaatt ttgtatgtgg attagaactt agggtttttg cgaatatcaa
     7321 ctattaaata tattactaat ttatacaaaa atataaagtt tgataaagtt atttatttga
     7381 ttataaaaat agggtaaaat atagatatat tgtattaatt aaattattcg aggtgtcata
     7441 tgaaaaaatt cattggatca gttttagcta cgacattaat tttaggggga tgttccacga
     7501 tggaaaatga atcaaaaaaa gacacaaaaa cagaaacaaa atctgtacca gaagaaatgg
     7561 aagcttcaaa atatgtaggc caaggctttc aaccgcctgc agaaaaaaat gcgattgaat
     7621 ttgcgaagaa gcatcgtaaa gaatttgaaa aagtaggtga acaattcttt aaagataact
     7681 ttggactaaa agttaaagct acaaatgttg taggtaaaga tgatggtgta gaagtttatg
     7741 tgcattgtga agatcatggc attgtattta atgcaagtct acctttgtac aaagatgcca
     7801 tccatcaaaa aggatcaatg cgcagtaatg acaatggtga tgatatgagt atgatggtgg
     7861 gtacagtgct gagtggcttt gaatatcgag cgcaaaaaga aaagtatgat aacttatata
     7921 aattcttcaa agaaaatgaa aagaaatatc aatatacagg ctttacaaaa gaggcaatta
     7981 acaagacaca aaatgtcgga tataaaaatg aatattttta tattacatac tcttctagaa
     8041 gtttaaaaga atatcgaaag tattatgaac cactgattcg aaaaaatgat aaagaattta
     8101 aagaaggaat ggaacgagca agaaaagaag tgaattacgc tgcaaataca gatgctgttg
     8161 ctacactttt ttctactaag aaaaacttta ctaaagacaa tacagtagat gatgtaatcg
     8221 aactaagtga taaattatat aatttaaaaa ataaaccaga taaatctaca atcacaatac
     8281 aaatagggaa acccactatt aatactaaga aagcctttta tgatgataat cgtccaatag
     8341 aatatggggt gcacagtaaa gatgaataaa attaatgata gggatttaac agaattaagt
     8401 agttactggg tttatcaaaa tattgatata aaaaaagaat ttaaagttaa tggaaaaagg
     8461 tttaaacaag tagacagtta taatgatgat aagaatagta atttgaatgg tgctgctgat
     8521 attaaaatat atgagttatt agatgataaa agtaaaccaa ctggtcaaca gacaataatt
     8581 tatcaaggaa catctaatga ggcaattaat ccaaataatc cattaaaatc atcggggttt
     8641 ggagatgatt ggctccaaaa tgctaaatta atgaataatg ataatgaaag cacagattat
     8701 ttaaagcaaa cagatcaatt atcaaatcaa tataaaataa agttagaaga tgcagataga
     8761 ttatcaaata gtgatttttt aaaaaaatat agaatggaat caagtaactt caaaaacaaa
     8821 accattgtgg cggatggcgg taattcggaa ggcggtgcag gagcaaaata tcaaggagcg
     8881 aaacatccga atgaaaaagt tgttgctact gactcagcaa tgattcctta tgctgcttgg
     8941 cagaaatttg ctagaccacg ctttgataat atgattagtt ttaatagtac caacgattta
     9001 ttaacatggt tacaagatcc attcatcaaa gatatgccag gaaaacgcgt taacattaat
     9061 gatggtgtgc ccaggttaga tactttaata gacagccatg taggttataa aaggaagtta
     9121 aatagaaaag ataacacata cgatactgta ccactaatca aaataaagtc ggtaaaagat
     9181 acagaaatta aaaatggaaa aaagtaaaaa agactattaa cataacatta gatatggatg
     9241 ggcgaattcc aataaatgtt tggacaggag attcgattgc acgttctgga agaggaactt
     9301 taattaaact taatttagaa aatcttgatg cgttgagtaa actgattact ggtgaaacaa
     9361 gtggtatgtt agcagaatgc gtaatctttt taaatgaaag ttttaacatc tcagaaaatg
     9421 aaaataaaaa ttttgcagat agaaagaaac aattatcaga aggatttaag gataagatta
     9481 acttatttca gttagaagaa atggaaagaa ctttaattag taaaataaac tcacttgaag
     9541 aagttgcaga tgaaacaata gaaagtatta gtgctgttaa acacttatta cctgattttg
     9601 cattggatgc attaaaagaa agaattaatg agttgtttaa aggtataaaa tcttttatag
     9661 aaaaagtgta tgatagtata gataatgaaa ttttagaaat tttcaaaaat atagatcacg
     9721 acttcagaga tggagtatct gaagaaatga tgaaacattt gaaagtagtg aaacagaata
     9781 tagaccaaat aaaaaatcaa aatgatattt atggtaggca aattgcagat attagaagta
     9841 ttatgaaaca acaagatgca acaattttag atggaaattt tcaaattaat tgtagcggcg
     9901 aaaatatggt acagggtcta gttatacctt ctaattattt aggaagaaaa atgaaaatat
     9961 taaaagacca tatcgatgat ggtattaaaa aaatagcaga ctatgttcaa agtatatatg
    10021 atgaatatgc atcgaaaatt gtcgatgtaa taaaatattt gattaataca attcccaaaa
    10081 tacgtaagaa tttaagacat gcaattgaaa tgttaaatgt aaaaaagaaa gaatttttgt
    10141 ccctgattcc taatgtaact tgtaattata ttaaaactaa attagaagaa ttagataata
    10201 ctttaggcaa atgggagcct tttcttaatg atttaaaagc agtgtcacca attttagata
    10261 accatttaga tgatattgtt aagaacatga agcctttgat tgtacaaatg atatttgaac
    10321 catcacatta tgatgatatg tttatttcaa gaaaagcttt aacgccagtg ttctcaagcg
    10381 ttttataaag cttgtaaaaa atataagggc aaaaaaaggg cggatttaag ctaacttgga
    10441 atgttttcga gtttttgagt tagttctcta tccatttttt cagttacatg agtatatatg
    10501 cgaatg
//
LOCUS       DANRDI010000092        10315 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.092, whole genome shotgun sequence.
ACCESSION   DANRDI010000092 DANRDI010000000
VERSION     DANRDI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.092"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..538
                     /locus_tag="PZ377_001791"
     CDS             <1..538
                     /locus_tag="PZ377_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDM6358661.1"
                     /translation="VQPKGERMKSKGMVLYMNRNTKTTTGKYIVSETLHDEDGRPKSK
                     DKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGSFKDLSKYKDGDISYNPEVP
                     SYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGDLKGSSVGYKDIEFTFVEKK
                     GENTFFTDSLHLEPSEDK"
     gene            632..2869
                     /locus_tag="PZ377_001792"
     CDS             632..2869
                     /locus_tag="PZ377_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDM6358662.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            3020..4198
                     /locus_tag="PZ377_001793"
     CDS             3020..4198
                     /locus_tag="PZ377_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDM6358663.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            4200..5588
                     /locus_tag="PZ377_001794"
     CDS             4200..5588
                     /locus_tag="PZ377_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6358664.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(6081..7742)
                     /locus_tag="PZ377_001795"
     CDS             complement(6081..7742)
                     /locus_tag="PZ377_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDM6358665.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(8068..9843)
                     /locus_tag="PZ377_001796"
     CDS             complement(8068..9843)
                     /locus_tag="PZ377_001796"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDM6358666.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(10017..>10315)
                     /locus_tag="PZ377_001797"
     CDS             complement(10017..>10315)
                     /locus_tag="PZ377_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDM6358667.1"
                     /translation="FMLIFIPEIINDIHGATFTSIVSVLYLAIFPTIIPYVLLAYIVK
                     SVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGIITLLGVSLSNFFQNT"
BASE COUNT         3433 a         1677 c         1866 g         3339 t
ORIGIN      
        1 tgttcaacct aaaggggaac gtatgaaatc aaaaggtatg gttctatata tgaatagaaa
       61 cactaagact acaaccggga agtatatagt gagtgaaaca cttcatgatg aagatggtag
      121 acctaagagt aaagataaag aatatcccgt taaaatggta gacaataaaa tcattccaac
      181 taaaggtatc aaagacgaaa atataaaaaa agaaatcgag aattttaagt tcttcgcgca
      241 atatggcagc tttaaagatt tgtcgaagta caaagacggc gatatatcat ataatccaga
      301 ggtaccgagt tattcagcaa aatatcaatt aactaatgat gactataatg taaaacaatt
      361 acgaaaaaga tataaaatac caacgaataa agcaccaaag ttattgttga aaggttcggg
      421 agacttaaaa ggttcatcag ttggatataa agacattgaa tttacttttg tggagaaaaa
      481 aggtgaaaat acttttttta ctgatagtct acatcttgaa ccgagtgagg ataaataatc
      541 atccacacac acgattcaat atgaaattca aatatgttgc tgtaaaataa tgtaaaataa
      601 acgtattcat attactgaat tgagggatag tatgcaacgt gattatttaa ttcgagtaga
      661 aactgagagt atgtcagatt tcaaaaggct caatagttta atgattggtt ttgttattaa
      721 aggtgaggca cacatttatg atgaaaataa tatgacgcaa tgcaacagtg gcgacatttt
      781 catcattaac caccgcgact tgtatcgatt tcaacttcaa caagatggca tcatatgtta
      841 tatccaattc caaatgaaat atttagcaga caagtttgat gatgtgcatt gtctatattt
      901 tcacttaaca gatgcgacca caacaaagaa tatacatcaa ctgagaaata taatggcaag
      961 actggtttca acacatatcc gacataatga gttgtctaaa ttgactgagc aacaacttgt
     1021 gattcagttg cttatgcata tgattcatta tgtcccgcgt acatatcatt cgaaccaaag
     1081 tatcttaaat gatgataaag tgaatcaagt atgcgactat atcgagttac attttcatga
     1141 agatttaagc ctttcagaat taagcgaata cgttgggtgg tcagagagcc atctgtctaa
     1201 aaagtttaca gaatcgctag gtgtaggatt ccatcatttc ttaaatacga cgcgaattga
     1261 gcatgcgaaa ctcgatttga catacacaga tgaaaccatt actgatattg cattgcaaaa
     1321 tggcttttca agtgcagcga gctttgcgag aacatttaaa cactttacgc atcaaacacc
     1381 taaacaatat cgaggtgatc gtccagcaat cactgaaaac cagcaatcgg cacaacatga
     1441 ttatcacgac cgtgaattga tattactttt aaatgactac attgaagaaa tgaatcattt
     1501 tattgaagat attgaaaaga tgaactataa agagattgcc tttcaaccaa ccaatcacca
     1561 actaaatcaa tttaatcata ttattcaagt gggctatttg aggaatttgc tcaatacaca
     1621 gtatcaatca cagttgctta catgtcatca tgattttcaa gtcaatgaag tattagcata
     1681 tgatgtgatt ccatatatca tgaaaaagct caatgcgcca ttcacgtatg atgcagaaat
     1741 ttcgaatata ttttatgata ttgatttgtg tttagacttt ttattagatc ataactttag
     1801 tttgaccatg catttggatc agtatgactc acgtgattat atcgatgcat ttaaaatttt
     1861 tatccatcac gttgccctgc atgtcagtca tagaaaagat ttgaagttca acttgtatgt
     1921 gacgacattg cacacgtctt tgattgaaat gattgattat tttaaagcat tattccctaa
     1981 tggtggcttg tacattcact tagatcaagc tacggaaaga catctaccat tattgaaacg
     2041 acttgagcca cacatcgacc attttgtatt tgatgccaat tcaaatgacg ctgttgattt
     2101 taataaaatg aatgatgatg aatttaaaac cgcgagtcaa atgattatta ataaaacgaa
     2161 ttaccttatc gacttaatgc atcgtcatcg cctaaagcgt ccactcattt tactcaattg
     2221 gaatacattg acgggtgata catttattac aaacggcgaa tattttagag gtggtatcat
     2281 cattgagcaa ttattaaaat taagttctaa agtagagggt atcggttatt ggttgaatta
     2341 tgatttgcac gtcagtcatt gtaaaaatga acgggattat atgaattcta ttgaactatt
     2401 tcatcaatat aatggaaaac gtccggtcta tttcacggca ttgctattta ataaattaac
     2461 aagcaatatt ttgtattctg atgatacatg tattgtcacg gggactgatt caaattttca
     2521 aatattgtta tatgatgcaa agcattttaa tccgtactta gcgttggaca atcaaatgaa
     2581 tatgcgtgcg acggaaatga ttcatttgaa cattaatgcc ctggaagaag gtatgtataa
     2641 gattaaacat tttaccttag ataaagaaaa tggtgcgtta tttaatcttt ggcgcaaaca
     2701 tcatacgata catggcatgg acaaggactc tatagattac gttaatcgaa tgagttttcc
     2761 gaaattagaa gtatatgata tagatatcac ggacacactg gcattaaaca ttaaaatgat
     2821 tacgaatggg attcacttaa ttgaagtaaa acgttaccca agttcataaa atgatcacaa
     2881 atcacaaatt ttgatataca taatttgtga ttttttatat tcaaagctaa aattgcaaaa
     2941 aattaatggt taacatctct gttgttggca atataaataa tgattaatca tttatgatgt
     3001 aactaaggag atgaaggata tgaatcaaca attaattgaa actttaaaat ctaaagaagg
     3061 caaaatgatt gagatcagac gttatttaca tcagcatcca gaattatctt ttcatgaaga
     3121 tgaaacggcg aaatacatcg ctgaatttta caaaggtaaa gatgtggaag tagaaacgaa
     3181 tgtcggacca cgtggaatta aagtaacgat tgattcaggg aaacctggta aaacattagc
     3241 aatccgtgca gactttgacg cattacccat tactgaagat acaggattat cttttgcatc
     3301 acaaaataaa ggtgttatgc acgcatgtgg tcacgatgca catacagcat acatgcttgt
     3361 attagcagag acgcttgctg aaatgaaaga tagttttaca ggaaaagtcg ttgtgataca
     3421 tcaaccagct gaagaagtac caccaggtgg tgctaaaaca atgattgaaa atggtgtatt
     3481 agacggtgtt gatcatgtat taggtgtaca cgtcatgagc acaatgaaaa caggtaaagt
     3541 gtattacaga cctggttatg ttcaaacagg acgcgcattc ttcaaattga aagttcaagg
     3601 taaaggtggt catggttcat caccacatat ggccaatgat gccattgttg caggtagcta
     3661 cttcgtcaca gcgttacaaa cagttgtatc tagacgacta agtccatttg aaaccggtgt
     3721 tgtcacaatc ggttcatttg acggtaaagg tcaattcaat gtcattaaag atgttgttga
     3781 aattgaaggt gatgtacgtg gattaacaga tgctacaaaa gcaacaattg aaaaagaaat
     3841 taaacgttta tcaaaaggat tagaggatat gtatggtgta acttgcacct tagaatataa
     3901 cgatgattat ccagcattat ataatgatcc agagtttact gagtacgtgg ctaagacgtt
     3961 gaaagaagca aaccttgatt ttggtgttga aatgtgtgaa ccacaaccac cttcagaaga
     4021 ctttgcatat tatgctaaag aacgcccaag tgcctttatt tatacaggtg cagcggtgga
     4081 aaatggtgaa atttacccac atcatcatcc taaatttaac atttcagaaa aatcattact
     4141 tatttctgca gaagcagtag ggacagttgt tttagattac cttaaaggag ataactaaca
     4201 tgaatgaaac gtatcgcggg ggcaacaagt taatcttagg tattgtatta ggtgttatta
     4261 cattttggtt gtttgcacaa tcacttgtaa atgttgtacc gaatttacaa caaagttttg
     4321 gtacagacat ggggacaatt agtattgcgg tcagtctaac tgcactattt tcaggcatgt
     4381 ttgttgttgg agcaggtggt ctggcagata aaattgggcg cgtgaaaatg acgaatatcg
     4441 gtttattatt aagtattatt ggttcagcat taattattat tacgaattta ccggcattat
     4501 taattttagg tcgtgttata caaggtgtat cagcagcgtg tattatgcct tctacattgg
     4561 ccattatgaa aacttattat cagggtgctg aacgtcagcg tgccttaagt tattggtcta
     4621 tcggttcttg gggtggcagt ggtatctgtt cactcttcgg tggtgcagtt gcgacaacta
     4681 tgggttggag atggattttc atcttctcaa ttatcgttgc cgtactttca atgttactca
     4741 tcaaagggac gcctgaaacg aaatcagaaa ttaccaatac acataaattt gacgttgcag
     4801 ggctaattgt tctagtagtt atgttgctaa gtttaaacgt tgtcattact aaaggtgcag
     4861 cacttggtta cacatcatta tggttctttg gtttgattgc aatcgtaatt gtagcattct
     4921 ttattttctt aaatgttgag aaaaaagtag ataatccact tattgatttt aaattatttg
     4981 aaaataaacc atatacaggt gcaacgattt cgaacttctt attaaacggt gttgcaggta
     5041 cattaattgt agcgaataca ttcgtgcaac aaggtttagg ttatacagca ttgcaggcag
     5101 gatacttatc aattacttat ttaatcatgg tgttattgat gattcgagtt ggtgaaaaat
     5161 tattacaaaa aatgggttct aagcgaccaa tgttattagg tacattcatt gtggtcattg
     5221 gtattgcact tatttcatta gtattcttac caggcatatt ttatgttatc agttgtgtcg
     5281 taggatattt atgtttcgga ctaggcttag gtatttatgc aacaccttct acagatacag
     5341 ctatttcgaa tgcaccgtta gataaagttg gcgttgcttc aggtatttat aaaatggctt
     5401 catcacttgg tggcgcattc ggtgtcgcaa ttagtggtgc tgtatatgct ggtgcagttg
     5461 ctgcaacgag cattcataca ggtgcgatga ttgcactttg ggttaacgta ttaatgggaa
     5521 tcatggcatt tatcgcaatt ttattcgcga ttcctaatga tgataaacgt gtcaaagatg
     5581 cgaaataata acgatgacac gccaagtgtt ttgataatga tataaatgaa agtaaagcaa
     5641 attaaatgag gaaatgtata gagcagtgca tatagataaa tctatgattg gattaacact
     5701 aatataaata taaatgaaaa tgaaaaacca tccacttaaa tttttgataa gttaggtgga
     5761 tggttattta ttgcgttgtt ctaaaaattt ggacatttat atggaaagaa ctatatataa
     5821 tgtgaaggtt atggcaattc gcttcaatga aggcgacgtg taaaagtgta tattaaacgt
     5881 ttcggggtca gactccgaga acagtgtccg tcatgcttac cgcatactaa tgaagcatta
     5941 ttatatcagt gtttatagcc gatttaagat aataaatttt gctaaacaaa aaagccaacc
     6001 catgaatgtt ggattggctt tttacatgca tctgaatctc taattttaaa aaaatatgaa
     6061 tataaataag acagtaaaaa ttaaatttca gttgttgcaa tttcttcatc tgtaggtaca
     6121 tcatcgttaa ggccaacaag tgcttcagaa acatttcgtg aatgataacc gatacgttca
     6181 agaacaccaa tcatatcgat atatagtaat ccgccttttg ttgtacattc accacgatta
     6241 aggcgtttaa tatgaccttt gcgtagttta tgttcaatat taaatgattc tctactacgt
     6301 tctacaattt catctttttt cgttttgtca taaacatcta acatgtcgat ggctttatca
     6361 aatgactcag caacatggtt gaataattta tccataccgc gttgtgcatc ttctgtaatg
     6421 cgaatatctt catcatgttg gcgttttaat tgagcgacat actcttctgt tagctctgct
     6481 acttttaaaa tagagcgatt gacatcaaac ataactgcta aacgctcaac gtctgccttc
     6541 gtaatggctt ttgtagaaat tctaactaaa taatttcgaa tgctatcatt gattgtttca
     6601 acagcttgat gcttttgttc aagctttttg atcaattttt tatcgtcttt tgtaatttcg
     6661 cgaatgtctt caaacattga taagacaatc tgacccacat tttgtaattc tttttgagtt
     6721 tcttgtaatg caacaccagg tgcgtgataa acaagatctt tgtttaagtg ctgaggttta
     6781 tagtcatcag caatatcttt acctgggaca agctttgtaa caatccatgc taaacctgct
     6841 acaaatggta attgaatcaa agtatttgtt atgttgaaga taccatgtga tactgcaatc
     6901 gtcatcgctg gttttaagtg ccataaatct tgtaacaaac taatcaaatg aatcacaact
     6961 ggcaagaaaa ttgtgaagat aattaccccg attaagttaa agatgacgtg tacaagcgcc
     7021 gcacgttttg cagcgattga gccggctaaa ctagctaaga tagctgtaat cgtggtacca
     7081 atgttatcgc ctagtaacac agggattgct gcgtttaagc taattaaatc ttgttgataa
     7141 aattcttgta aaataccaat cgtcgcactt gaactttgaa ctagtgctgt taaccctgcg
     7201 ccgacaatga cagcgagtat tggatttgta gacatatcaa gcattaattg cttaaatcca
     7261 tctaatgatg ctaaaggttt aacggcatca cccataaatt ctagaccgaa gaatagtgaa
     7321 ccgaaaccga atagtatgcg gccaatgtta ttgattttag agcgtttaaa gaaaaagatt
     7381 aagaatgcac ctaatgctaa aattggcatt gcatattcgc ctaaatctat accgataata
     7441 aatgcagtta ccgttgttcc gatattagca cccattatca ctccaatggc ttgtttcaat
     7501 gtcataaatc cagctgttac cagtccgatt gtgataactg tcgtacctga actactttgt
     7561 attaaaatag ttacaacgat acctgcaata acacctaata ctggatttga tgtaaatttg
     7621 tttaaaatat ctcgtagcct gtctcctgct gatgcttgaa gcccgtctcc catgattttt
     7681 aagccgtaaa ggaaaatacc taaaccacct aaaaaggaga aaatgacttc tgtaaccgac
     7741 atttccatta tttcacctca aataagcttt atatttagat tatcgcttat aattgtaaat
     7801 ttaatgttaa gattaggtaa aattatttaa caatatatgt tatttgtata tgacttgtaa
     7861 aatatcgtca cttattatgt aaattttcag tgtgaaatgg caggtttgca atcacttgtt
     7921 taacaaaatg atgcaatcaa tcatgtaatt atgtttcatc aaaaaaatca tgagagtgga
     7981 acaacgaaat aatttttatg aaaacatcat ttctgtccca gtctcatgat ttgaaatcac
     8041 caaataaaaa tctattaatg gttttcgtta taacaatttg tgttctttta ataatgactc
     8101 aatgtacgta ccttttatct ttttaaggaa tcctgctaat gcgagttttt gcattttcga
     8161 atctttagta atctcacgca aatcttggtg gtcattcagt tcgtatatgg catccattaa
     8221 gacgcgaaga tcaaatggac tattgatgac ttctggaata ccacgatcta tatttagtaa
     8281 ttgataaaca gcttccatgg cagtacgaac cgaatattct gttgtaaata cagtgtctcg
     8341 ctctgtttct gcaaagttac caataaatgc taagttctga gattgatgcg ggacgactaa
     8401 aggtctgtcg ccgatagcac gcgtcatgaa ataagatgtg atatatggca tataaacagg
     8461 aatcgtatta gatgcatgtt ttgctaagtc ttcaattttg tcagttgata cacctaagtg
     8521 atacagccat tcttggcata tttcattacc actacattct gtaattggct ttttaatata
     8581 atcgccgttt acatctgaat ataaggcata aatccatgta gatatttcat tttcaggttg
     8641 gtctttaaac tgttgctgac gattgattgt aaaactcatt tgccatgcag aatcattgat
     8701 tgtaataata ccgcctgtaa ctgttttgcc tgcaagtggg tcacgtttac aaatactttc
     8761 tattgtatcg ataatctctt tattgtttgt tgtagaagtt gctgaaacaa accaactttt
     8821 tttaggaata ttttggcaaa acttatcagg attaccaaat tcaggacttt gtcgcgctaa
     8881 atttttccat agtgtccaac taccacctaa ttcgtcagtt ggtggcgctg gtgtatcatt
     8941 atcaccataa gtagagcttt ctgtaatact accgtttgtc acaaagacaa gatcgtttat
     9001 agtcagttta atagattctg cattaccatt acggtcaatt aatatttctc gggcaatttt
     9061 ttgacttgtc gtaacatcta ttttaatatc ttcgactttt acatcgtatt caaattgaac
     9121 cccatgcgat tttaaatatt caaccatagg taatactaaa gattcatatt gattatattt
     9181 agtgaatttt aaagctgaaa agtctgcgag accactaata tgatgaacga atcgcattag
     9241 atagcgacgc atttccattg cagaatgcca cggttcaaat gcaaacatcg ttttccagta
     9301 aatccaaaag tttgaattaa agaagtcatc ggaaaataca tctgttattt tgacatcatc
     9361 taaatcttct tcattcgtta agcataaatc taaaatttct ttaatcgccg ttttagtcaa
     9421 agtgaagtct ccgtctgtga ctaaacgttg accctgtttc tcaataacac gacagcgaga
     9481 atagttaggg tcttctttgt ttagccaata gaactcatct aatacagacg cgttatcgat
     9541 ttctaatgaa gggatagatc tgaataagtc ccacaaacat tcaaagtggt tctccatttc
     9601 acgaccaccg cggacaacat agccttttaa aggcatattt tcaccatcaa gactaccacc
     9661 tgctttaggt aactcttcta aaatatgaat cttcgaacct tccatttgac catcccttat
     9721 taaaaaacaa gctgcagcaa gtgaagctag accagatccg attaagtaag cggatttgtt
     9781 ttctacattt tcaggttttt tagggcgcgc aaatgcttca taatttccat aactgtaata
     9841 catatccacc aagtcctttc gtattagaat acactcagac tatacccctt tgtgaattgc
     9901 aaatattagt atttatattg gtaaatttga cgaaattaga tcatttttat atttttgatt
     9961 tttaaaattt tatcactcga atgtttttgg attctgaggt gcgggacttg gaataattat
    10021 gtattttgaa agaagttact taaactaacg cctagtagag tgataattcc gcctataata
    10081 gcaagggttg ttggtagctc gtctaataac agataagata ataataaaga aacgataggt
    10141 gttaaataaa gagacattgt tgcatcagag acaccaactg acttcacaat ataagcaagc
    10201 aaaacgtatg gaattatagt agggaatata gctaaataaa gtaccgatac tattgatgta
    10261 aaagtggcgc cgtgtatatc gttgatgatt tcaggaataa aaataagcat aaatg
//
LOCUS       DANRDI010000093        10198 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.093, whole genome shotgun sequence.
ACCESSION   DANRDI010000093 DANRDI010000000
VERSION     DANRDI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.093"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..152
                     /locus_tag="PZ377_001798"
     CDS             <1..152
                     /locus_tag="PZ377_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDM6358668.1"
                     /translation="ELSDKLYNFKNKPEKSTITIQIGKPTINTKKAFYDDNDPIEYGV
                     YRKDE"
     gene            209..1147
                     /locus_tag="PZ377_001799"
     CDS             209..1147
                     /locus_tag="PZ377_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDM6358669.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     DASKYVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVE
                     VFVHCDDHDIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKY
                     DKLYKFFKDNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLN
                     KSDSEFSKELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEI
                     PNGIELSIKFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            1346..1465
                     /locus_tag="PZ377_001800"
     CDS             1346..1465
                     /locus_tag="PZ377_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358670.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      1548..1682
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1744..2289
                     /locus_tag="PZ377_001801"
     CDS             1744..2289
                     /locus_tag="PZ377_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDM6358671.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(2395..2643)
                     /locus_tag="PZ377_001802"
     CDS             complement(2395..2643)
                     /locus_tag="PZ377_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDM6358672.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            2751..3704
                     /locus_tag="PZ377_001803"
     CDS             2751..3704
                     /locus_tag="PZ377_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDM6358673.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            3694..5073
                     /locus_tag="PZ377_001804"
     CDS             3694..5073
                     /locus_tag="PZ377_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDM6358674.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            5226..6686
                     /gene="ebpS"
                     /locus_tag="PZ377_001805"
     CDS             5226..6686
                     /gene="ebpS"
                     /locus_tag="PZ377_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDM6358675.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            6811..6927
                     /locus_tag="PZ377_001806"
     CDS             6811..6927
                     /locus_tag="PZ377_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358676.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            7088..8074
                     /locus_tag="PZ377_001807"
     CDS             7088..8074
                     /locus_tag="PZ377_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDM6358677.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(8189..9157)
                     /locus_tag="PZ377_001808"
     CDS             complement(8189..9157)
                     /locus_tag="PZ377_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDM6358678.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            9234..9893
                     /locus_tag="PZ377_001809"
     CDS             9234..9893
                     /locus_tag="PZ377_001809"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDM6358679.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            9924..10040
                     /locus_tag="PZ377_001810"
     CDS             9924..10040
                     /locus_tag="PZ377_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358680.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
BASE COUNT         3857 a         1533 c         1741 g         3067 t
ORIGIN      
        1 ttgaactgag tgataaacta tataatttta aaaataagcc agaaaaatct acaattacaa
       61 ttcaaatagg aaagcctact attaatacta aaaaagcctt ttatgatgat aatgatccaa
      121 tagaatatgg agtgtatcgt aaagatgaat aaattttttt aaaatgcttt ttaatattct
      181 atataaacac ataaatgggt gatgagctat gtttaaaaag gcgaaattaa tcttaatagc
      241 aacgctactg ttatcaggat gttcagcgat gaataatgaa tcaaaaaaag acacaaatac
      301 agaaacaaat acagaaacaa aatctgtacc agaagaaatg gatgcttcaa aatatgtagg
      361 acaaggattc caaccacctg cagaaaaaga tgcgattgaa tttgcaaaga agcataaaga
      421 taaaattgct aagcgaggcg aacaattttt tatggataac ttcggtctaa aagttaaagc
      481 tacaaatgtt ataggtagtg gcgatggtgt agaagtattc gtgcattgtg atgaccacga
      541 catcgtattt aatgcgagta ttccatttga taaatcaatt attgacagtg atagctcatt
      601 aagaagtaag gataaaggtg atgatatgag tactttagtt ggtgcagtac tcagtgggtt
      661 tgaatatcga gcacaaaaag aaaaatatga taaattatat aaatttttca aagataatga
      721 agagaaatat caatatacag gatttacaaa agaagcaatt aataagacgc aaaatagtgg
      781 ttatgaaaat gaatattttt atatttcggc cataccttat aatttagctg agtatagaga
      841 ctattttgaa ccattgttaa acaaaagtga cagtgaattt tcaaaagaat tgtcaaatgt
      901 taagaagcaa ttaaaagata agtctaaagt ttcggtaact actactctat ttagtaaaaa
      961 aaagaactat actaaaaaaa gtaacagtga aaatgtaata aaaatggcag aagaaataaa
     1021 aaaagataaa gagataccaa acggtataga gcttagtata aaattttcgg acaataaaat
     1081 aaatacggtt aaaccaaatt ttaacggtga aagcacttca gaatatggtg tgtttgatca
     1141 agaataaaat taatgatgaa aatttaacgg agaatagtgt atattgagta gatcaagaat
     1201 aaaaagataa ttctactatt gttgtgaagg caaataagta gaagatttta agtgtaattt
     1261 ctggtgattt aaataataat ataaatggaa gtactgatat aaaacttttt aacctactag
     1321 attcttataa tttgctttcc attttatgac gatttttact caattgagtg atagaatcaa
     1381 aaaagccatc tcaaaaatta atcaagcaaa caacattcca aacaatgctc gcaaatcacc
     1441 aatgtatcac tctccaatta cgtaactatg atttaattta agcatagtta ttgaggtttt
     1501 gtgatatata gtataaaatt aatgagaatt aaatttaata atgtaaaatt catcttcggg
     1561 gtcgggtgta attcccaacc ggcagtaaat aaagcctgcg acctgctagt atgtatcata
     1621 ttagtggctg atctagtgag attctagagc cgacagtata gtctggatgg gagaagatgg
     1681 aggttttttg ttgtgcaata atcctcctat tcttacgaga tgaatggaag gagaaaattg
     1741 aatatgcaac aaaataaacg tcttatcaca ataagtatgt tgagcgcgat tgcgtttgtg
     1801 ttaactttta tcaagtttcc tataccattt ttgccaccat acttaacttt agattttagt
     1861 gatgtaccgt cactactagc tacatttacg tttggaccag ttgccggtat cgtagttgca
     1921 ctggttaaaa atttattgaa ctacttattt agtatgggcg atccagttgg accatttgct
     1981 aactttttag caggcgcaag tttcttatta actgcttacg ccatctataa aaataaacgt
     2041 tcaacaaaat ctttgattac tggattaatc attgcaacaa tcgttatgac tatcgtgttg
     2101 agtattttga actatttcgt tctattacct ttgtacggta tgatatttaa cttagctgat
     2161 atcgcaaata atcttaaagt aatcattgtt tcaggaatta taccattcaa tattattaaa
     2221 ggtatcgtta tttctattgt atttatttta ctatatagaa ggcttgcgaa tttcttgaaa
     2281 agaatttaat caaattaaag caaaataata tacacataat aataaaaagc aggtgactat
     2341 caataaacga tagcttgcct gctttttcta tagaacattt gtctaaaaaa tcaattattc
     2401 aaattttaaa gcgtccccat caaatgattc gtctgcaatt ttaatagaat ctgtagggca
     2461 tccatcaatt gcatcttcca tatcttcata taattcctca ggtacttctg cagtaccttg
     2521 gttatcgtca aggattacga aagcaatacc ttcgtcgtcg taatcatata tatctggcgc
     2581 tgctgcaccg catgcaccac atgcaataca agtatccata tcaacgattg tatattttgc
     2641 caatgtcttc gcctcctttg ataaaaatgc taaaatagta atgtgactaa aattttagac
     2701 agcatcattt ttattttcaa attatccgtt ttacagagtg agggttaaat ttgcaacaca
     2761 ttataaaaac agcattacaa caaacattta actataaaac aaataaaagt atttacaata
     2821 tcttagttgg taagaaatct caccaaacct tttttgacgc ttgtagtcaa caacagttgt
     2881 cattatatca cagtttacca ctattaaaat atccgtcttt tgagctattt ttagaaaaaa
     2941 tcaatgaatt taatgctgaa atggaaatca tgttgcatcc tagatataca tttgaaagca
     3001 tggggcaaac atttcaagca attcaactat tagtgcaaac catgtctaat accaaacaac
     3061 atgtttttca ttttgtacca atctctcaaa ataataagat acaagaaact gtaaagattg
     3121 tctataacta cattaaagaa aaccaattac aaattgattt tgaaaatgaa ctacataact
     3181 tgtttaaagc aataacttta aaagggccat gttacttaca ttattatttg caaggctatg
     3241 atgaaccaat gtatacgaga cagcaagtta gtttaataga aaagctatct caacagcaat
     3301 tgtttgaata cgaaatgaat aatttagtga caatgatgtt tgaattagaa agtggagaat
     3361 atactatttt atcaaaaata ataatgaaac ctacattatt aaatcaaact tatattactt
     3421 atacaaaatt gcttgaacaa ttcacgatgg aagatatagc ggctcaacaa caagttaaaa
     3481 tcaatactat cgaagatcat gtacttgaaa tcttaatcaa aggttacatg tctaattacg
     3541 atgattatgt tgaactagaa gatcaactcc agtttttgaa tttttatcaa cagcatcgtg
     3601 gcgaacgatt aaaattttac aaagaacaat ttgacacgtt atcatatttt caattaaaag
     3661 tattaatcgt tggatttgaa agaggtgatc tgaatgttgc atgatatttt acgaaacaaa
     3721 tttggattcg agagttttaa accgggacaa caggaaatta tagaaagtat aatgtctcaa
     3781 caacacactc taggtatact tccaactgga agtggaaaga gtttgtgtta tcaaatacct
     3841 acgtatttat caggtaagcc gacattaatt atctcaccgt taatatcttt aatggatgac
     3901 caagttatgc agttgaaaat aaatggagaa aaacgtgtaa catgtattca ctctggtatg
     3961 gatgaaattg agaaaaagca taatattaaa tgtttacgac atagccgctt catctttcta
     4021 agtccagaat ttctcctgca accgtcaaat tttaaattaa tatctatgat agactttggc
     4081 atgattgttc tagatgaagc acattgccta tctgaatggg gatatgattt cagaccacat
     4141 tatgctctaa taggaaaagt aacaaagcat tttaaagaag cggttgtctt agcattgaca
     4201 gcaactgcac caccgcattt acaagatgat ttgacggaaa tgttagcgat tcaattcaat
     4261 gttattaaaa ctacaatgaa tcgcccaaat ataagcttta agcatcttaa ttttcatgat
     4321 gatgaagata aaattgaatg gttgctgccg tttctacaac agtcgggacc aacgattatt
     4381 tatgtctcat cgaaaaagat gtgtctgaat ttagcgcaac ttatttatga ttcaggtttt
     4441 cttacaggta tttatcatgg tgatatgaat tatcaagagc gacacacagt tcaacaacaa
     4501 tttttaaata atgatattcc gattatagtc gcaacgagtg cttttggtat gggaattaat
     4561 aaaaaagata ttcgcacaat cattcacttt catctttcaa caagtccttc taactacatt
     4621 caagaaattg gccgtgcggg tcgcgatggt gaactaagtc aggcaattag tttattccaa
     4681 ccggacgata aatatatttt agaaacgtta ttatttgcag atatgataac agaagaagat
     4741 gtacaaaatt tcgaaatagg agaattttta gctcccgata aacaagccgt tttgacaacg
     4801 ttgcaatcat tctatagtat cggcgccttg aaacagatat ttaagcaatc atttaaacga
     4861 aagcaattag gattctttcg catgattggc tattgcaaat tggatcaatg tagacggaag
     4921 tatttattag aatttttcgg tgaatatcca ccggcacaag atcgatgttg tgacaatgat
     4981 tctaatataa ctgatatcgc aattttaaat aagaagaagg taattagaag tattggattt
     5041 gatgaaaagt tgcaaaattt atttctcaga tagtattact ttactaaaag aaaattgaca
     5101 agctataatt agtgtataca caattgaaaa atgattgaaa taattttgaa aaatatacat
     5161 aaacatatgt catgtgggta tattttatgt aaaatcattg taatagaata gaaaggaaga
     5221 tggctatgtc taataatttt aaagatgact ttgaaaaaaa tcgtcaatcg atagacacaa
     5281 attcacatca agaccatacg gaagatgttg aaaaagacca atcagaatta gaacatcagg
     5341 atacaataga gaatacggag caacagtttc cgccaagaaa tgcccaaaga agaaaaagac
     5401 gccgtgattt agcaacgaat cataataaac aagttcacaa tgaatcacaa acatctgaag
     5461 acaatgttca aaatgaggct ggcacaatag atgatcgtca agtcgaatca tcacacagta
     5521 ctgaaagtca agaacctagc catcaagaca gtacacctca acatgaagag gaatattata
     5581 ataagaatgc ttttgcaatg gataaatcac atccagaacc aatcgaagac aatgataaac
     5641 acgatactat taaaaatgca gaaaataaca ctgagcattc aacagtttct gataagagtg
     5701 aagctgaaca atctcagcaa cctaaaccat attttacaac aggtgctaac caatcagaaa
     5761 catcaaaaaa tgaacatgat aatgattctg taaaacaaga tcaagatgaa cctaaagaac
     5821 atcataatgg taaaaaagca gcagctattg gtgctggaac agcaggtgtt gcaggtgcag
     5881 ctggtgcaat ggctgcttct aaagctaaga aacattcaaa tgacgctcaa aacaaaagta
     5941 attctggcaa ggcgaataac tcgactgagg ataaagcgtc tcaagataag tctaaagatc
     6001 atcataatgg caaaaaaggt gcagcgatcg gtgctggaac agcaggtttg gctggaggcg
     6061 cagcaagtaa aagtgcttct gccgcttcaa aaccacatgc ctctaataat gcaagccaaa
     6121 accatgatga acatgacaat catgacagag ataaagaacg taaaaaaggt ggcatggcca
     6181 aagtattgtt accattaatt gcagctgtac taattatcgg tgcattagcg atatttggag
     6241 gcatggcatt aaacaatcat aataatggta caaaagaaaa taaaatcgcg aatacaaata
     6301 aaaataatgc tgatgaaagt aaagacaaag acacatctaa agacgcttct aaagataaat
     6361 caaaatctac agacagtgat aaatcaaaag aggatcaaga caaagcgact aaagatgaat
     6421 ctgataatga tcaaaacaac gctaatcaag cgaacaatca agcacaaaat aatcaaaatc
     6481 aacaacaagc taatcaaaat caacaacagc aacaacaacg tcaaggtggt ggccaaagac
     6541 atacagtgaa tggtcaagaa aacttatacc gtatcgcaat tcaatactac ggttcaggtt
     6601 caccggaaaa tgttgaaaaa attagacgtg ccaatggttt aagtggtaac aatattagaa
     6661 acggtcaaca aatcgttatt ccataatata actatataaa ttgtaactga actgctatat
     6721 acaaacgtgc atattataag tatctctaaa tattttggag aacttaaatt gcgcttataa
     6781 gtatgtagcg gttttttcat ttttcaaagt ttgttattta acaaggtctt ttctcgaata
     6841 ttggcatatc aatttaactt tttaaatagt catcaaaaag ataaaacacc acaatcaaca
     6901 aatttaacga ggaagaataa aaaataaatc aacatattaa attgtagtgt tattcaactc
     6961 cgtagctaac aattctctat gcacattaaa caaattgtca aaaatatatc ataaatcttc
     7021 aagcacagac ttagcgcatc aatcactgaa ctgttataat agtttgggat taaaggaggc
     7081 cgaaacaatg caaaaagttg aaagtatcat aattggtgga gggccatgcg gattaagtgc
     7141 ggctattgaa caaaaaagaa aaggtattga taccttaatt attgaaaagg gtaatgtcgt
     7201 tgaatcaatc tacaattatc ctactcacca aacatttttc tcatcaagtg ataaattaag
     7261 tattggggac gtaccgttta tcgttgaaga aagtaaacca agacgtaatc aagcgctagt
     7321 ttattaccga gaagttgtaa aacatcatca attaaaagta aatgcatttg aagaagtatt
     7381 aactgttaaa aaaatgaata ataaatttac tattactacg acgaaagatg tttatgaatg
     7441 tcgattttta acaatcgcga caggctatta tggtcagcat aatacattag aagttgaagg
     7501 tgcggattta cctaaagtgt tccattattt taaagaggca catccgtatt ttgatcaaga
     7561 tgttgtaatt atcggtggta agaattcggc tatcgatgct gctttggagt tggaaaaagc
     7621 tggtgctaac gtgacggttc tatatcgtgg tggagattat tcgccttcaa ttaaaccgtg
     7681 gatacttcca aatttcacag cattagtaaa tcatgaaaaa attgacatgg aatttaatgc
     7741 taatgttacc caaataactg aagatactgt gacttatgaa gtaaatggtg aaagtaaaac
     7801 gatacacaat gattatgtat ttgcgatgat tggttatcat cccgattatg aatttttaaa
     7861 atctgtaggc attcaaatta atacaaatga atttggaaca gcgcctatgt ataataaaga
     7921 aacatacgaa acaaatatcg aaaattgcta tattgcaggt gtaattgctg cagggaacga
     7981 tgcgaatacc atttttattg aaaatggtaa attccacggg ggcattattg ctcaaagcat
     8041 gctagctaag aaacaaacgc ccttagaatc ataaaaataa aggtctatgt actaaataac
     8101 ttagttttac aacgactgac attcatgata tgtcaaatga ggttgatgac taatgattgt
     8161 acatagacct ttttatgtta cgtattcatt ataattcaaa atatgatttg atttcagctt
     8221 tatctaaatt gttgcttaac gcgactaata attttaatct tgcttttgga ccattcaagc
     8281 cgttagaaaa aataaaacct tgttgtgcga gttggtaacc accaccatcg tatgcgtaag
     8341 ttggactcac aataccatta aaggaacgtg aaactagcac aataggtata tttaaagata
     8401 ctaattgttg aatgccttct aatgcgcttg gaggtatgtt gccttgtcct aacgcttcaa
     8461 taaccatacc atcgatacct tctcgactat aaaaactaaa aatgtcacct ggcataccca
     8521 tatatgcttt tactaatggt acatttagtt tgtcattgac attttccaat gcttgttggc
     8581 gatatggcat atgatggaat tgcacacgat cctttgtcaa tacacctagc ggaccatgat
     8641 ttggactttg aaatgtgttt gtattagacg tatgtgtttt ggtaacatta cgcgccgtat
     8701 gaatttcatc attaaataca accatcacgc ctttatgacg ggccttttca tcagaggcaa
     8761 cgcgaatagc ggaaatataa ttatataatc cgtcagaacc aatttcatta gacgagcgca
     8821 ttgcgccagt aataacaaca ggttgctcga tacctaatat taaatcaagt aaaaaggcag
     8881 tttcttctaa cgtatcggta ccatgcgtga taacgaaacc atcataatat ttatttgtta
     8941 ctgcttctaa aataatatcc tttaattgtt taacatgttg gattgtcata tgaggtgatg
     9001 gtacattaaa aggatttaat tcatctattt gtgcatattg atttatgaca tcttgatgca
     9061 ttgaaatagg gttgatatca tttgttacta ctttattaga ttggtcttgt gacatactaa
     9121 tggtgccacc agtatgaata acaagtagat gtttcatata tttcctccta tatttaattt
     9181 acctaattat gataaaatat tattcataaa acgacaagga agggaaatga cgcatgaaag
     9241 ccattaatat tgcattagat ggtccagctg ctgccggaaa aagtacaatt gcgaaacgtg
     9301 tagccagcga actatcaatg atttatgtcg atacaggagc aatgtatcgt gcattaacat
     9361 acaaatattt aaaattaaac aaaactgagg actttgcaaa actagttgac caaacaacat
     9421 tagatttaac ttataaagca gataaaggtc aatgtgtcat tttagataac gaagatgtaa
     9481 cagacttttt aagaaataat gatgtgacgc aacatgtttc atacgttgca tctaaagagc
     9541 cagtacgttc attcgccgtt aaaaaacaaa aagagttagc tgcagaaaaa ggtatcgtaa
     9601 tggatggtcg cgatatcgga actgtagtgc taccagatgc agatttaaaa gtatatatga
     9661 ttgcatcagt tgaagagcga gcagaaagaa gatataaaga taatcaatta agaggtatcg
     9721 aatcaaattt tgaagattta aaacgtgata ttgaagctcg tgatcaatat gacatgaacc
     9781 gtgaaatatc accattaaga aaagcagatg atgcagtgac attagatacg acaggcaagt
     9841 cgattgaaga agttactgac gaaattttag cgatggtgag tcaaattaaa taatctaata
     9901 agtcataact atctatttgt aatgtgcaac gcttaacgca tatacaaaat gaatgtgctg
     9961 ataatgattt actcaaatta aaaggtgatt tttattcaat gatgaatgaa agttgccttt
    10021 ttatttttgg taaaagttaa tgcgtcagtg aattgtgtaa gtttttcaaa aagtaaaaag
    10081 aaataataaa ggtgaattat tagaattcca aaaataattc attacattca taaagcattt
    10141 tacaaatggt aagaaaatga gtgttacaaa tctaaatatt gcaaaagaag ctgattta
//
LOCUS       DANRDI010000094        10143 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.094, whole genome shotgun sequence.
ACCESSION   DANRDI010000094 DANRDI010000000
VERSION     DANRDI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.094"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(19..273)
                     /locus_tag="PZ377_001811"
     CDS             complement(19..273)
                     /locus_tag="PZ377_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358681.1"
                     /translation="MLIKIRGGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDL
                     IYIFCTLLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
     gene            complement(429..1337)
                     /locus_tag="PZ377_001812"
                     /pseudo
     CDS             complement(429..1337)
                     /locus_tag="PZ377_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1562..1774)
                     /locus_tag="PZ377_001813"
                     /pseudo
     CDS             complement(1562..1774)
                     /locus_tag="PZ377_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(1849..3384)
                     /locus_tag="PZ377_001814"
     CDS             complement(1849..3384)
                     /locus_tag="PZ377_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDM6358682.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3435..4979)
                     /locus_tag="PZ377_001815"
     CDS             complement(3435..4979)
                     /locus_tag="PZ377_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDM6358683.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            5208..6164
                     /locus_tag="PZ377_001816"
     CDS             5208..6164
                     /locus_tag="PZ377_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDM6358684.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            6378..7151
                     /gene="phnC"
                     /locus_tag="PZ377_001817"
     CDS             6378..7151
                     /gene="phnC"
                     /locus_tag="PZ377_001817"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDM6358685.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            7153..7953
                     /gene="phnE"
                     /locus_tag="PZ377_001818"
     CDS             7153..7953
                     /gene="phnE"
                     /locus_tag="PZ377_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDM6358686.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            7950..8765
                     /gene="phnE"
                     /locus_tag="PZ377_001819"
     CDS             7950..8765
                     /gene="phnE"
                     /locus_tag="PZ377_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDM6358687.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(8896..10074)
                     /gene="deoB"
                     /locus_tag="PZ377_001820"
     CDS             complement(8896..10074)
                     /gene="deoB"
                     /locus_tag="PZ377_001820"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDM6358688.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
BASE COUNT         3320 a         1832 c         1566 g         3425 t
ORIGIN      
        1 ttacaagcgc attttcgttc agtcaattac tgccaatata acttcgtaga tcatagaaga
       61 ttgatttatt tcccagccta ttctttttat aatatttagc aaaggtaata atgatattaa
      121 taatgtacaa aaaatataaa tcaaatcgac atccttataa aacatcagaa ccactaaaaa
      181 caaaaaagca caaaataaaa ttaaatttaa aataaacgac cacttttcaa aaaaatctct
      241 tttcatattt ccacccctaa ttttaataag cattatttta tattctcttt taagtttatt
      301 attcaaaagg aaaacagaaa tatctttcaa tattattata aacatttcaa ctacttttaa
      361 aaaccaacaa aaaaatactt attttaagta gatgagcata agtgaacata gttctttagt
      421 tataataatt aattcaacca aaagtcgatt tgtttttgca attggttttc atttcctctt
      481 aaagatattt tcattaaatc tgtcaaatca atagacgcta tatttttcaa cttatctcta
      541 tatttatttt tagtacgtct ttctaaattt ccccattcct cttcttcgtg agttaataaa
      601 tgaagcattg ctcgttcttg tatattttca atcattttta aattcggttt taaaatatgc
      661 aaatcatcaa aacaatcttt ccaacaatca accatatctc gttttaattc aatttccaca
      721 cgccatagaa atgttgaatc aatttcaaca tctgcattat ctttacgttc ttgtttttat
      781 tataaatccg aataaaccta tcactattac gcacaccaaa atattttgtt tctggtttta
      841 cattacgtcc ataaaatata gttttcttta ccgacttatc tgacaatgca taatagtcat
      901 ttaaatcaaa ttcaaaatca aaagccaaat ctaatctcgt aaaactaaca tcgtccaaat
      961 aactgatgat attttgtttt aaccaaagca cttcatcatg cgaaagctta ttaggattaa
     1021 attcaacgcg catactacgt ctattccaaa gagttgcttt tattttgtca tattcaatat
     1081 aaactttttc tttaagagct ttagctttaa agtttgtttg taaaatatcc caaagccgaa
     1141 tttcaggatt agtactcata aaatgtgaaa gtctctctgc gttagacatg ctaagattcc
     1201 caacaatcgt tatagcgtca aaagacaatt ttggaatagc tagtgacatc ctatgtcgat
     1261 ttaaccggct attaccggat attagagtat ccagttttac aaatggatga aacgaaattc
     1321 aaaacactaa aaaatatgtt ccactaacag caaaaaaata ccattatgtt cctactaaaa
     1381 aactaaaaat actggagaac aaatgtcagg atataactta ggatactatg taataaaaat
     1441 ttacaataaa aaaacaggaa aacaaatttc aagtaaaaga atacccatac aaagaggata
     1501 aaataaaaaa cctcgaactg aaatgatgat cttttcagct cgaggtttaa atattggtgc
     1561 cttatttata tagattcgtt atattatatt ctctattttc attaacataa tccttaaaga
     1621 gttttaaatt aatacctgct agatgattca aaaatgtttc atcaactttt aaataattca
     1681 ataatttttg tggtgtcagt aaatttctat caaaatacaa ctttaataaa ctattcattt
     1741 tgacaggacg tgacatttca atcacgtcgt ctaaagataa tactttctcg ctttaaacaa
     1801 aaacaaaaac ttacccgatt aaaatcaagt aagttttaca tatatccgtc acttttcaac
     1861 tttaaaatca acaacttgcg ggatgcgcat caaattatta tttgataaaa aatcaataag
     1921 taattgtgcg ccttcaactt gaatatcttt tacaactggc gcgtcgatat acatatcata
     1981 ctgaccaccg cctactgcac gataattatt tacacaaatt gtatatgtct gctttaaatc
     2041 aactgcgtga ccttgaatca tcatattgct cacacgttgt ccctttggtc ttccaacatg
     2101 aatggtataa cttacgccac catatatatc ataattaaag tgttgtggtt tgggttcaag
     2161 gaagtctgcg ctcacactaa cttcatcatt tttcacgtca aaatattctg ctgatcgttc
     2221 aatggcttct ttaagtttgg caccacttac agctaaaact ttaaatgtat ttggaaatgg
     2281 gtaattgtta ataacatctc gcatcgtcac gacttgcttg aaaccactag cagaatcaaa
     2341 caaagctgta caggcaacat ctgcgtcact tttttctaat aaagcgtaat tcataaaatt
     2401 tgtaaaagga tgcggtgcca cacgtgcctc aaatgcatga ttaatcgtca tatcatatgg
     2461 caatgtagta atttcgtaat ctaaccagtc ctctaactgc tttcgtaaat gttggtcatc
     2521 ttcatcaata gtaaatgtgg aatcatctat aacaggaagt aattcacatg attcaacgga
     2581 tagattttca tattcatcag tactcaagac tactctgcct acagttgtac ctctcgtacc
     2641 aggttgaatc acagccgttt gcttaaacct ttcagcaatt tgtcgatgtt ggtgacccgt
     2701 aataaagata tctatatctt tagaaaacgc ttctaacatg gcatatcctt cattttcacc
     2761 cgttaatact tcggtcggcg taccactttc taaatccttt tcaaatccac catggtaaca
     2821 aaccacaatg atatctgcat gtcgcttcat ttcaggtaag tattgttgaa gtatttcaaa
     2881 agcactatga aacgtaagtg actgaatatg ctctggttgt tcccaatggg gaataaattg
     2941 tgtcgttaaa cctatcacac caacagtttg atctccaacc tgaaaatact tcacaccgtt
     3001 atcagtcaat gtactatcat tttcatatat attagcgcac aaaactggat aattgagtct
     3061 gcgtaaagtg tcttttaagt atggtaatcc ataattaaat tcatgattgc caagcgtacc
     3121 aaagtcgaat gccattcgat tataaaaatc aactaaaggc tggctactgc cgctatgcgc
     3181 gattaagtaa ttacaaaatg gtgacccttg caaaaaatca ccattatcta ttttaaaact
     3241 ttggtcatac tgccttctgt cttgttctat aacatgattc gctagtaaca atcccatagg
     3301 ttgatattga tttctactcg taaaatctgt tgggaaaata taaccatgta cgtcactcac
     3361 gacataaaat gctatgtttg acatcctcac tcactccttc aatcacaaac atctttctta
     3421 tttctattat atatttattt gaagtctgtt gtaatcaagg ttttgtcacc gagttttaaa
     3481 cgaatctttg aaccttccat actttcaagt actttagcat tgaccttaat tgtgacattt
     3541 ccgttttcat ctgctttaac tgttggcaaa gtactgtaac ctggtgggtt ataatcgtta
     3601 tctttacttg aaaattgtcc gatttgacgt ccgccttctt tgtaaatacc aactctaaga
     3661 ttttcaagtg tttgatttgc ttcaaatcct tttaaatgta ttgtcatttc aaatggctca
     3721 tttacagaaa cattttgcgg gatatcaaat gttacttttt cgttctgatt tggtggtgta
     3781 tgatcatctg gtgtgtttgg ctgaggatct gcgccttttt cgctgccata actacctgct
     3841 ttaaatgttg ttggatcata ccatttataa ccactcggcg gttgtgacca tggctctttt
     3901 tcaggctcag ttgaacgctc tggtcgttca aaatcaagca acttagtctt tgtatctaat
     3961 gttaggctac tcgccttaag tgatttccca tcattatctt tagacatcca agccgttata
     4021 ttatttaata gcttaccgtt gtcttgttct ttaaaaccat catatgtttt cttcttttct
     4081 ccattatctt ctcttacata tttgggcgaa ctatcttcca caagtgatga atcaccgata
     4141 aatgctgctt taccttttcc aactttagaa attgctacat aggggccttc tgctttaccg
     4201 cccccattat aaataccttg atctacagca tgtgaccatt tacttttcgc tggcaattgt
     4261 tctggtgtat acacaatacc ttttgctttc tctggattag taattgctaa tgtcgatccg
     4321 gcatgcatag agacagattt cacaccttca gtaataccga aactttcttt tgaagaaaca
     4381 atattgctcg tatttaaatc acctagtgca ttatatcgaa aacgtacgcc aaagtttgta
     4441 gataaccaat ctgaactttt cacaccttgc attgcagtag aacttttttc ttctgcattc
     4501 atacctttcg acatatcttc atatgctcca cgtcgataac cattcattgc ctccgatgaa
     4561 tcaatacgat ttaaatttcg gtcagcattg taatgatctg aaataaagac aacattgcca
     4621 ccttgtttca catatttaac aattgctgcc tgttctgatt ctttgaaagg aatgttagcc
     4681 tcaggaatta caaatatttt ggaacttttc aaacttgctt ctgttatgtt cgaatgacca
     4741 tcaatagctt taacgtcata accttgtttt tgtattgaat ccgcataatc tgaaaatgca
     4801 ccatcactaa cccaatctgc agcaccagct gtttgaccat gagaacgatc gaataatacc
     4861 gttcgctgtt gctttgtagg ttgcgattca tgcgttatag ctaaagattg cggtaaagca
     4921 cttaatgata ccgttgcaac aattgcagag acagttaatg acttatatat ttttttcatt
     4981 ttgtgaggct ccttttaaaa taaatttgtt cttgaattat aggataaaaa ttcgttgcat
     5041 atgagcaatt taacgaaaaa tttacaaaat cttatcaaac tcttaaagaa agttattaaa
     5101 attcattttt ataaaatact ttttaacatt taaatgtggt acgctataag tgtaatttca
     5161 ttgcatacat attacacgat taagaatgtg aaggggacag ttatcaaatg aaaaatttta
     5221 agtgtttatt tgtattaatg ttagcagtca ttgtttttgc agcagcatgt ggaaactcaa
     5281 gttctttaga taatcaaaag aacgctagta atgattcgga ttctaaatca ggaggataca
     5341 aacctaaaga attaaccgtt caatttgtac cttcgcaaaa tgctggaaca ttagaagcta
     5401 aagcaaaacc attagaaaaa ttactatcta aagaattagg gattccagtt aaagtgtctg
     5461 tatcaactaa ctacaataca attgttgaag ctatgaagtc taaaaaagtt gatgttggtt
     5521 tcttaccacc aacggcatac acattagcac atgatcaaaa agcagctgat ttattattac
     5581 aagcacaacg tttcggtgta aaagaagatg gttcagcaag taaagaactt gtagatagtt
     5641 ataaatcaga aattcttgtt aaaaaagact caaaaattaa aagcttgaaa gatttaaaag
     5701 gtaagaaaat tgccttacaa gatgtaacat caactgctgg atatacattc ccacttgcga
     5761 tgttaaaaaa cgaagcaggt attaatgcaa ctaaagatat gaaaattgtg aatgttaaag
     5821 gtcatgacca agcagttatc tcattattaa atggagatgt agatgctgcg gctgtattta
     5881 acgatgcacg taatactgtg aaaaaagacc aaccaaatgt atttaaagac acacgaattt
     5941 taaaattaac acaagctatt ccgaatgaca caatttctgt aagaccagat atggataaag
     6001 attttcaaga aaaattgaaa aaagctttta tagacattgc taaatcaaaa gaaggtcaca
     6061 aaattattag cgaagtttat tcacatgaag gatacacaga aacgaaagat tcaaatttcg
     6121 acattgtaag agagtacgaa aaattagtta aagatatgaa ataatcatta tttaacaaat
     6181 gaatcattag cgaatttggt attaaaagct ttcgttcaat agatatattc tagattaata
     6241 ttgaaaagct aggcgctaaa ctgaaacaga tatagaaagg tgtcgctgta catttgaaac
     6301 catttgtaca cagaaaccca atgtctatga tatttcagtt taccttggct tttctttatt
     6361 aaagaaaggt gtcaaacatg agtcaaatcg aatttaaaaa cgtcagtaaa gtctatccta
     6421 acggtcatgt aggcttgaaa aatattaact taaatattga aaaaggtgaa tttgcagtta
     6481 ttgtcggact atctggtgct gggaaatcca cgttattaag atctgtaaat cgtttgcatg
     6541 atatcacgtc aggtgaaatt ttcatccaag gtaaatcaat cactaaagcc catggtaaag
     6601 cattattaga aatgcgccga aatataggta tgattttcca acattttaat ttagttaaac
     6661 ggtcaagtgt attacgaaat gtactaagtg gacgtgtagg ttatcaccct acttggaaaa
     6721 tggtattagg tttattccca aaagaagaca aaattaaggc aatggatgca ctagaacgcg
     6781 tcaatatctt agataaatat aatcaacgct ctgatgaatt atcaggtggc caacaacaac
     6841 gtatatctat tgcacgtgcg ctatgccaag aatctgaaat tattcttgca gatgaaccag
     6901 ttgcttcatt agacccatta actacgaaac aggttatgga tgatttaaga aaaatcaacc
     6961 aagaattagg catcacaatt ttaattaatt tacattttgt tgacttggca aaagaatatg
     7021 gcacacgcat cattggttta cgtgatggtg aagttgtcta tgatggtcct gcatctgaag
     7081 caacagatga cgtatttagt gaaatatatg gacgtacaat taaagaagat gaaaagctag
     7141 gagtgaacta acatgccttt agaaatacct acaaagtatg actccctttt aaagaaaaag
     7201 gtttctttaa aaacgagttt taccttcatg ttaatcattg tgcttatcat ttggagcttc
     7261 atttatacgg gatttaattt tggagattta atgataggaa tacctcaaat aggtgatcta
     7321 ttcaaacaaa tgattccacc tgatttcgag tatttacaac aaattacaac gccaatgtta
     7381 gataccattc gaatggctat cgtaagtaca gtattaggta gcatcgtttc aataccaatt
     7441 gcgttattat gtgctagcaa tatcgttcat caaaagtgga tttcaatacc ctcgcgcttt
     7501 attttaaata tagttcgtac tattccagat ttgttattag cagcaatctt tgtggctgta
     7561 tttggaatcg gtcaaattcc agggatatta gcactgttta ttttaactat ctgtattatt
     7621 ggaaaattat tatatgaatc attggaaacg atagatccag gtccaatgga agcaatgacg
     7681 gctgttggcg ctaataaaat aaaatggatt gttttcggtg ttgtaccaca agccatatcg
     7741 tcatttatgt catacgtatt atatgcattt gaagtaaata tacgtgcttc agctgtgctt
     7801 ggattagtcg gcgctggcgg tattggattg ttttatgatc aaacacttgg tttatttcaa
     7861 tatccaaaaa cagcaacgat tattttattt actttagtta tcgtcgtcgt cattgattac
     7921 atcagtacga aagtgagggc acatctcgca tgacacagga aatagcaaaa tataatgttc
     7981 acacaaaagc acacaaacga aaattgatta aaagatggct tattgcaatt gtcgtcttag
     8041 ctattatcat ctgggcattt gcaggtgtac caagtttaga acttaaaagt aaatcattag
     8101 aaatcttaaa atccatattc agcggattat tccatcctga tatcagctat atctatatac
     8161 cagatggcga agacttatta cgtggtttac ttgaaacctt tgcgatagcc gttgtaggta
     8221 ctttcatcgc cgcaattatc tgtattccat tagcatttct aggtgcaaat aatatggtaa
     8281 agctacgccc agtttcaggt gttagcaaat ttattttaag tgttatacgt gtcttcccag
     8341 aaattgtaat ggcacttata tttatcaaag ctgttggccc aggttcattt tcaggtgtat
     8401 tagctttagg tatccattcc gtaggtatgc ttgggaaact tttagctgaa gatattgaag
     8461 gtctagattt cagtgctgta gaatcattaa aggccagtgg tgcgaataag attaaaacac
     8521 tcgtatttgc agtcatacca caaattatgc ctgcctttct atcactcata ctttatcgct
     8581 ttgaactaaa cttacgttca gcttctatac tggggctaat tggggctggt ggtatcggga
     8641 caccactcat atttgccatt caaacacgtt cttgggaccg tgtaggtatt atattaatcg
     8701 gtttagtact aatggtcgca attgtcgatt taatttccgg ttcaatccga aaacgtattg
     8761 tttaacatta aatcaggata ctcctaaata agaagtccta ccgtcttacg tttctctatt
     8821 ataataaaaa cagcagtgaa gaaaactatt gttatagtta acttcactgc tgtttttata
     8881 atatctaaat ttattctatt tcaattcctt taaataactt ttaccgaact ctggtaatgt
     8941 tacgttgaaa ttatctgcta tagttgcacc gatagaactg aatgtagtat cactttctag
     9001 tgcatgacca cctttaaatt tcggactgta cataattact gggatatatt ctctcgtatg
     9061 gtccgtacct ggcgctgtcg ggtcattacc atggtctgct gtaataatta ctaaatcgtc
     9121 ttcttttaag ttgctaaaca gttctggcaa gcgatcatcg aaatctttaa ttgcttgtgc
     9181 ataacctggt ttatcacgac gatgaccgta taatgcatca aagtctacta agtttaagaa
     9241 gctaatacct gtgaaatctt tcttaacaat tttcatcaat tgatccatac cgtccatgtt
     9301 actcttcgta cgaaccgctt ctgttacacc ttcaccatca taaatgtcat taattttacc
     9361 gatggcaata acatcataac caccgtcttt caaatgatct aagacagttt taccaaaagg
     9421 ttttaacgca tagtcatgtc gattagatgt acgtgtaaag tttcctggtt caccaacata
     9481 tggacgtgcg ataatacgac caattaaata tttagggtct tttgtcaact cacgaacctt
     9541 ttcacaaata tcatataact cttctaatgg gataatgtct tcatgtgcag caatttgcaa
     9601 tactgggtct gcacttgtat aaacaattaa gtcaccagtt ttcatttggt gctcgcccca
     9661 ctcatcgata atttgcgtac ccgatgccgg tttgttagca acaactttac gacctgtcat
     9721 ttcttcaatt tgttgaatta actcttcagg gaatccatta gggtatactt taaaaggttg
     9781 cataatattt aatcccataa tttcccagtg accagtcatt gtatctttac caactgaagc
     9841 ttcactcaat ttagtatagt atgcttctgg ttgttcaact gcatttacta ctggtaattt
     9901 atcgatgttc cctagaccta acttttcaag gtttggtaaa gtttgatcga aaccttctaa
     9961 ggtatgtctt aaagtatgtg aaccttcatc tttaaaatca gctgcgtctg gcgcttcacc
    10021 aatacctact gaatccatta cgattaaatg tacacgatta aatggtcttg tcatagctat
    10081 cactcccaaa atttatatat attagtaatc tgaatctgct tctaaacctt gcataatttg
    10141 aac
//
LOCUS       DANRDI010000095        10098 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.095, whole genome shotgun sequence.
ACCESSION   DANRDI010000095 DANRDI010000000
VERSION     DANRDI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.095"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(14..841)
                     /locus_tag="PZ377_001821"
     CDS             complement(14..841)
                     /locus_tag="PZ377_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6358689.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(844..963)
                     /locus_tag="PZ377_001822"
     CDS             complement(844..963)
                     /locus_tag="PZ377_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358690.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1075..2076
                     /locus_tag="PZ377_001823"
     CDS             1075..2076
                     /locus_tag="PZ377_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDM6358691.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEVYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2198..2662)
                     /locus_tag="PZ377_001824"
     CDS             complement(2198..2662)
                     /locus_tag="PZ377_001824"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDM6358692.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2675..3856)
                     /gene="ribB"
                     /locus_tag="PZ377_001825"
     CDS             complement(2675..3856)
                     /gene="ribB"
                     /locus_tag="PZ377_001825"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDM6358693.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(3867..4499)
                     /locus_tag="PZ377_001826"
     CDS             complement(3867..4499)
                     /locus_tag="PZ377_001826"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDM6358694.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4506..5549)
                     /gene="ribD"
                     /locus_tag="PZ377_001827"
     CDS             complement(4506..5549)
                     /gene="ribD"
                     /locus_tag="PZ377_001827"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDM6358695.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5676..5811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6030..7532)
                     /locus_tag="PZ377_001828"
     CDS             complement(6030..7532)
                     /locus_tag="PZ377_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDM6358696.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     gene            8055..8369
                     /locus_tag="PZ377_001829"
     CDS             8055..8369
                     /locus_tag="PZ377_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDM6358697.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            8369..9661
                     /gene="arsB"
                     /locus_tag="PZ377_001830"
     CDS             8369..9661
                     /gene="arsB"
                     /locus_tag="PZ377_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDM6358698.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            9679..10074
                     /gene="arsC"
                     /locus_tag="PZ377_001831"
     CDS             9679..10074
                     /gene="arsC"
                     /locus_tag="PZ377_001831"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HDM6358699.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
BASE COUNT         3225 a         1786 c         1464 g         3623 t
ORIGIN      
        1 cctttttaga atattaaatt tctacaattt cgtcatcttc aacaataaag cccattgtat
       61 tgacgctgtt atttaagaaa gtcagaatat aacgcattac ttcatcacgt tctggctcat
      121 tgtgaacctc gtggtaaaaa ccttgccaag ctttaaaata taattcaggt gtttgatatt
      181 tttctttaaa ctcatcaatt gccctagtat caacaattaa atccttcgtt ccatacatta
      241 atagcgttgg cattggttga atgtcatgaa tatgagccat cgtatctttc atcgtctcat
      301 taattgtatt ataccaatga tacgttgctt tttttaacat taaaccatcg ttaactgttt
      361 cttcaacaat ttctaaatta cgtgttaaat cttttggttc tacaccaaca ttaaaacgtg
      421 tgtcttttga aattttacct atatttgaaa caagtttatc tttacgattt tttccattct
      481 tttgaagttc tagcatagga gaaattaaca tcatcccctc gattggcaat tctacttttt
      541 caagtaaatt taataaaatc aaaccgccaa gtcctacccc taatacataa gtaggaattt
      601 tatattcatt agctatcttt aaccagtcta gcaaactttc gtgatacgtt tgaaagtttt
      661 caatttgtcc tttattagct cttgaagttt gaccttgacc aggcaaatct cccataatca
      721 catgatagcc atttcttctt aacatcgtaa taacatatgc atatcttccc gtatgttcta
      781 atatattatg agcaataaca acgacgcctt tcgcatcatt ttcagcttcc cacttccaca
      841 ttattatact gccccttttt cattaatctt caataacata attatagcaa attcactatg
      901 tagatttcta tttatagtat tattgttgtc catattatta tatataaatg aaatcaacat
      961 caataatagt gtaattatac ataattattt ttgattgttt ttgatgaaaa cgctttctcg
     1021 aatatttttt tcatgctaaa cttattgtaa acacaagggt ttggaggagt agcaatggca
     1081 ctattaaaga atttttttat cggattatct aataatagtt ttttaaacaa cgcagcaaaa
     1141 aaagtgggcc cacgtttggg cgccaataaa gtcgttgccg gaaatacaat tccagagtta
     1201 attaatacaa tcgaatactt aaatgacaag aatatcgctg ttacggtaga caatttaggg
     1261 gaatttgtcg gtacagttga agaaagtaat catgctaaag aacaaatttt aacaattatg
     1321 gacgcgcttc atcaacatgg cgtaaaggca catatgtctg ttaaattgag tcagttaggt
     1381 gcagaattcg acttagaatt agcttaccaa aatttaagag agattttact taaagcaaat
     1441 acttacaaca atatgcatat aaatattgat actgaaaaat atgctagcct gcaacaaatt
     1501 gttcaagttt tagatcgctt aaaaggcgaa tttagaaatg ttggtactgt aattcaagca
     1561 tatttatacg atagccacga attagttgat aagtaccaag atttacgatt acgtttggtt
     1621 aaaggtgcat ataaagaaaa cgaatcaatt gcatttcaat ctaaggaaga cgtagatgca
     1681 aattacatca aaataattga acaacgtttg ttaaacgcac gcaatttcac ttcaattgca
     1741 acacatgacc atcgcatcat taatcatgta aaacaattta tgaaagaaaa tcacattgaa
     1801 aaagatcgta tggaattcca aatgctctat ggttttagat cagagttagc agaagaaatc
     1861 gcaaatgaag tctataattt cactatttat gtaccttatg gcgatgattg gtttgcgtat
     1921 tttatgagaa gattagcaga acgcccacaa aacctatctc ttgctgtaaa agaatttgtg
     1981 aaacctgctg gcttaaaacg tgttggcata attgcagctt taggagctac agttatgtta
     2041 ggtttaagta caattaaaaa attatgccgt aaatagagca agacataaac aataatttag
     2101 gagtctggaa caataatcaa tgttctaggc tcctaaatgt tatattggca gttctgaaac
     2161 atttataaat ctttgattat gaactcacaa cagtgctcta tgcttttata gattttaata
     2221 aattagccat ttcaattgca cttactgctg cttcagcacc tttattgcca gctttcgtac
     2281 ctgctctttc cacagcttgt tcaatacttt cagtcgttaa aataccaaat atgactggta
     2341 cattagtttg atcattcact ttagaaacac ctttcgcgac ttcattacaa acataatcat
     2401 aatgagacgt agcaccgcga attacgcatc ctaatgtaat tactgcatca taatttcctg
     2461 atgaggctaa ttttttagct actaaaggaa tttcaaacgc acctggcaca aatgctacat
     2521 caatattgtc ttcattaaca tcatgtcgaa tcaaagtatc ttttgcacct tcaagtaatc
     2581 ttccagtgat aaaatcatta aatcgactaa ctacgattgc aactttcaaa tcttttccaa
     2641 ttaatttacc ttcaaaattc atgttaaaat cctcctatat taaatgaccc atttttattt
     2701 ttttcgtttc catataatca tgattatgta ccgtttctgg tacgataact tcaattcttt
     2761 ctgcaatatc aatgccatat tgttttaatc cctcaaattt acttggatta ttacttaata
     2821 aattgatatg ttcgatgtta aaatatttta aaatctgtgc agcaatatga taatctcgca
     2881 aatcttcatc aaaacctaat gctaaatttg cagttactgt atcatatcct tgctcaatta
     2941 attcatatgc gcgtaatttg tttaacaatc ctatgccacg accttcttga ggtagataaa
     3001 taatcatgcc accatgttca ttgatatact tcatagacga ttcaagttga gcaccacaat
     3061 cacaacgttg actatggaaa atatcgcctg taaggcacgc agaatgtaag cgtacatttt
     3121 catgttgtcg aattgcacct tttgtcagta caactatctc ttcatctgtg tatgtcgctt
     3181 taaaaccata catatcaaat gttccgaaat ctgtaggcat tttcactttt gccttaaatt
     3241 caatttctgg ttctaatttt ttacgatatt caattaaatc atcaatcgta atcatcttta
     3301 attgatgttt ttctttaaac ttttgtaaat cttgtccttt cgccatcgtg ccgtcatcat
     3361 tcataatctc acaaatgaca ccagcgggct tggcaccagt aagtttagct aaatcaacag
     3421 ccgcttctgt gtgtccattt ctagctaata cgcctttatc ttgtgctact aatggaaata
     3481 aatgaccagg acgattaaaa tctttagctt cactactagg atcaatgagc tttttggcag
     3541 tcaatgtacg ttcataagca ctaattcctg ttgttgtatc tacatgatca atactcactg
     3601 taaattgcgt accaaagatg tcggagttat catcaaccat ttgtaccaaa tccaaacgtt
     3661 gtgcaatatc tttagacact ggtgcgcata ttaatcccct tgcttctttc gccataaaat
     3721 taatggtatt atcgttcatc cattcagtaa ccgctactaa atcaccttca ttttcacgat
     3781 tctcatcatc tactacaata attggttctc cattttttaa agccattaaa gcactgtcaa
     3841 tattatcgaa ttgcatgcta cccctcctaa aaaccaaatg ctcttaattt atctacagat
     3901 aattggtctt tatctttatt taaaatattt tcaacatatt taaacaaaac gtctgtttct
     3961 aaatgtactt tatctcctaa ttttttggat gataaaatcg ttgaacgcct cgtttctgga
     4021 ataagatgaa tgtcaaaact gttatcatgc ttatcaaata ccgttagact aacaccatcc
     4081 acagtaatag acccttgctt aactaactga ttattaatat gttggctaca ttgaatcgta
     4141 ataatttttg cattggctgt ttcatttatt tttgaaactg ttcctagttc atctacatga
     4201 ccgaggacaa aatgtccacc aaacctaccg ttaccactca tggcacgctc taaatttact
     4261 tctgattgtc gcttaacatc tgctaaatag gttttatttt cagtgccttt aattacttga
     4321 acagtaaaag atgtctgatt aaaatcaatc actgttaaac atgcaccatt aacactgatg
     4381 gaatcaccaa tatgcatatc tgccgtaatc ttatgtgctt caatttcaat cgtcctgact
     4441 gattgacgaa tttgaacact tttaacgaca cctatttctt caacgatgcc agtaaacatg
     4501 catcatcact tctttcgtaa agttaattta acattttgat ttaataactc ggaatgaaca
     4561 atttcaaatt ggttcgcatc tggtatctca atcacatcat ttgtttgata aaattgataa
     4621 tttccagatc cgccaattaa tttcggggca taatagagaa taaattcatc tatataatta
     4681 gattggagaa attctgaagt agtggttgga cctgcctcaa ctagcaaagt tccaactcct
     4741 cttttatata aattgtgaag aattgttgtt aaatcgcaag acttcaagta aataatttca
     4801 atatgtgttt gattggttgt taaatttgga ttttcagtat atatccaaat tggtgttgat
     4861 tcatcttgat aaatttgctg attaaaatga atattcccag acttagacaa tattactttt
     4921 atagggtttt ttccatcttg aatacgtgta gtatattgtg gatcatctaa ttcaactgta
     4981 cgtcttccag ttaacactgc gtcgtgtcga tgtcttaact tatagacatc ttgtttaacc
     5041 tctttgttag taatccattg actttgtcca ttatcattcg cttgtttacc atctaaactt
     5101 gcagatactt tcactgtaat ttgtggcagt tgctttgctt ttgctttaaa aaagtcttgg
     5161 tataattgtg atgcccgttc atcatcaacg cattcaacct caataccgtg agcccgtaac
     5221 gtctcatcac catgtgtgtc taacgaattg tcttttgttg cgtatactac ttttgctatc
     5281 ttacaatcaa ttattttgtt aacacagggt ggtgttgaac caaaatgact acatggctct
     5341 aacgtaatat aaatcgtcgc accttcagca ttttgttgtg ccatatcaag tgcttgaacc
     5401 tccgcatgct tgtcaccttt tctcaagtgt gcaccaatac caacaatcct accttcttta
     5461 actacaacag cgccaacggg tggattaaca cctgtttgac cttgtaccat atttgcaagt
     5521 tgaatcgcat aatccataaa ttgactcaaa tgatcacctc tataaacaaa aatcctcaca
     5581 tcatgaatta agatgcaagg agaaaaattt atcgttaaat aagcctattt gtacacattt
     5641 ttacaaatac gctacattat ctttgtcgat aattaacatt ctttctccca tccagacttt
     5701 aactgtcggc tctagaatct cactagatca gccactaata tgaaacatat tagcaggtcg
     5761 caggctttat ttactgccgg ttgggaattt caccctgccc cgaaagaatt atatatgaaa
     5821 ttgttataga ttatttgagt acgtagtatg tcaactacat ttaaaatgat actatatgtt
     5881 ttctgaaaaa acaattaatg acggttttaa tttaatataa tctgagtact ataggcatct
     5941 cattgatatg attcttacta acagacatta aaatcaaacc ttcaattcgt ctctatagag
     6001 cgttctcttt attatcttct agttacaaat tattgattgt cactgcgctg ttgttgctca
     6061 ttcgattcta aagcatcata taattgagat actgtatgcg caacttgttc tacaatcatt
     6121 ttcacaccgt ttcgtagttt attaacaccg tttgtcattt gacctatcgc aatcatattt
     6181 gttaatgttc caaaccttgg actaataact tgattggttt ccggaatgat ttgtatgcct
     6241 cccattgggt gtgcttgtac aatttgtcta ttttcaagat ttctaattaa ttgatcatct
     6301 tgatccaatt catttaaatg actttttgca cctgtcgcgt taatgacaac attatatatg
     6361 tctactgatt cttggttttt gtatgaaaaa taatacaact tgccatcatg tttcacatct
     6421 tctaaatctt ttttcaaaat taaagactta ttttctatta attcaataat tagttcagca
     6481 gttcttggag gcattggatt tgaatttaat tgaatcatct ttgagtattt ttgattaaat
     6541 tgatgttggt cttcaatact taagctattc catatccaat ttaaattctc tttcaaatgt
     6601 tcaatcatac tttggaaaat gcccatttct gttggacgcg ctaaatcata cttcaaatct
     6661 gcaatatgat ttcctgtacg tctatgtact aattttttaa aatcaatgtc ataatcagca
     6721 cattctttta aaaataaaga aactaaagta tcaagcggtg cattgccgaa atgatgtttt
     6781 ttaatgtcat ttaatttgtc tttagttaag tacttgaatg tcacgtctat cattgtacct
     6841 cttacacttg gtaaatgagc agaacgactc gtcatagtaa ttggtaattt tggatgatga
     6901 gcagcaacat aacggacaac atctaaactg gcaaggcctg taccaataat cgcaatatcg
     6961 tccagttcat ttacttcgtc taacgtatta tatgttggat aaggcgtagc gatatatcct
     7021 tttttaccct ttaagttata tggatcatgg taggcaaatg taccacatgt taaaaataca
     7081 taatcgtacg cttgccatga ttgtcctgaa tttgtagtac atatgtaata agttaaattc
     7141 gtttcatcga tattagaatt tgtataaatc tcttgaactt tattataatt agttgatata
     7201 tttggatatt ttttcgtgaa catagataaa taagatttca tataatgtcc gaatacaaat
     7261 ctcggtaaat atgcaggttc atcaaaatta aaatcagttt gttgtttata ccacttccaa
     7321 aattcagtct catcatctaa atttaaactc atctttttcg aaggcatatt aattagcagc
     7381 tcagaactat cattttgaaa tggtacgccc tgtcccatat ttactttatc atcgtataaa
     7441 tctatatcta attgattaaa cttcgggtgc ttaactaact ctctcaatac acttacacca
     7501 gcagttccca tgcctattat tgctacacgc atatatcatc catccttttg taattcaaaa
     7561 tattatattt aaactattac ctataattat agcaatttgg tatttccaat aaaatttatt
     7621 aaggtaagct attatttcac tttcacaacc attaaaccat ttaaaacttt tcatttaata
     7681 tcaaccttag tgtttgtgtt aaaatatttt taacgaggtg aattaaattg aaatttatag
     7741 caaagatatt aatctcatta gtaattctat ttatgatttt tagtcgtaaa aacaaagctt
     7801 cttaatcaat acccttgatt acaaaataaa ttcaactaag gtgattggcc atacactcaa
     7861 gatatggcta atcacctttt tcaatgcaga acaaagagta tattttgcaa aatcaatatc
     7921 tataatttat ctttaatacc cttttaatac taaaaccaaa tttaaattac atagtcatat
     7981 cgtgtaatat accaaaggaa catagacatt catctatata gatgataatc tatttaacgt
     8041 ctaggaggta tgttatgacg tataaagaac tagcaacatt tttaaaagtt ttatcagatt
     8101 caagcagatt agaaatacta gatttacttt cttgtggaga gttatgcgct tgtgatttgt
     8161 tagcacattt tcaattctct caacctacac ttagctatca tatgaaagca ttagtaaaaa
     8221 ccaacttagt tacgacacga aaaatcggaa ataaacattt ataccagctc aatcataata
     8281 tttttgagtc cgtaattaat aacttgtcta aggttcatac ctctaaccaa cgatgtattt
     8341 gtcataacct taagactggt gaatgctaat gatgacaact ttagcgacac tcatttttct
     8401 agtaacttta ttatttgtat tatggcaacc taaaggccta gatattggca ttaccgcact
     8461 aactggtgcc tttattgctg ttattactgg tgttgtaagt ttttccgatg ttttcgaagt
     8521 aacaggtatt gtttggaatg ctactttgac ttttgtctca gtcattctta tttcattaat
     8581 attagataaa gttggattat tcgaatggtc agctattcac atgcttcatg cttcaaaagg
     8641 caatggttta aaaatgttcg tttatatcat attattgggt gccattgttg ctgcattttt
     8701 cgcaaatgat ggcgcagcgt taatcttaac gcctattgta ttagcgatgg ttaaaaatat
     8761 aggttttagt aagcgggcca tattcccttt tattattgcg agtggtttta tagctgacac
     8821 aacttcttta cctttaatcg tgagcaatct agtgaatatt atatctgctg attattttca
     8881 tgtaggattc gttcgatatt ttagtagaat gattatacct aatttattct cgcttttagc
     8941 aagtattata gtattgtggt tatattttag aaaagcgata cctaaaacgt ttgatgataa
     9001 taatataaag catcctaaag atgccattaa tgatttaaag ctatttaaaa tttcatggat
     9061 tgttctagtt atattacttt tcggctatct aatcagtgaa tttactaaaa ttccggtatc
     9121 aattttcact ggaatcattg cttttatttt tctaatgttg gctcgtaaat caaatgctgt
     9181 aaatattaag caagtcatta agggcgcacc ttggaatata gtattatttt caattggtat
     9241 gtatatcgtc gtattcggct taagaaatgc tggcattact ttaatattgg ctaaaatatt
     9301 agaatatatt tccaattacg gtctatttag cactattttg ggaatgggct tcatttcagc
     9361 gtttttatca tcaataatga ataatatgcc tacagtttta atagatgcga ttgctattgg
     9421 tcaatcaaat gtccatggca tgttaaaaga aggcctaatt tatgcgaatg ttatcggttc
     9481 tgatttaggt ccaaaaatta caccgatagg ctctttagct acattactgt ggttacacgt
     9541 cttaacacaa aaagatgtta agatttcttg gggcacatac tttaaaactg gtatcatcat
     9601 tacaattcca gtactattta taaccctcat agggttgtat ctaacactta tcatatttta
     9661 attaattagg agattgttat gactaaaaaa acaatttatt ttatatgtac aggcaactca
     9721 tgtcgaagtc aaatggctga aggttgggct aaacaaatct tagcggatga ttggaatgta
     9781 tattctgctg gtatcgaaac acacggtgtt aatcccaaag cgatagaagc tatgaaagaa
     9841 gtaggcattg atatatcaaa tcatacatca gatttaatcg ataataatat tattaaaaat
     9901 tcaaatttag ttgttacatt atgtagtgat gcagacgtaa attgcccttc tttaccaaca
     9961 aatgttaaga aagaacattg gggatttgat gatcctgcag gcaagccttg gtcagagttc
    10021 caacgtgtaa gagatgaaat taaaatcgca attgaaaatt tcaaatcacg atgagagtgg
    10081 gacagaaatg ataaaaaa
//
LOCUS       DANRDI010000096        10092 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.096, whole genome shotgun sequence.
ACCESSION   DANRDI010000096 DANRDI010000000
VERSION     DANRDI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.096"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..149
                     /locus_tag="PZ377_001832"
     CDS             <1..149
                     /locus_tag="PZ377_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDM6358700.1"
                     /translation="EVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            149..679
                     /gene="mreD"
                     /locus_tag="PZ377_001833"
     CDS             149..679
                     /gene="mreD"
                     /locus_tag="PZ377_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDM6358701.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    706..833
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            846..1154
                     /gene="rplU"
                     /locus_tag="PZ377_001834"
     CDS             846..1154
                     /gene="rplU"
                     /locus_tag="PZ377_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDM6358702.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            1160..1480
                     /locus_tag="PZ377_001835"
     CDS             1160..1480
                     /locus_tag="PZ377_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDM6358703.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            1492..1776
                     /gene="rpmA"
                     /locus_tag="PZ377_001836"
     CDS             1492..1776
                     /gene="rpmA"
                     /locus_tag="PZ377_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDM6358704.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            2131..3423
                     /gene="obgE"
                     /locus_tag="PZ377_001837"
     CDS             2131..3423
                     /gene="obgE"
                     /locus_tag="PZ377_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDM6358705.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            3436..3891
                     /locus_tag="PZ377_001838"
     CDS             3436..3891
                     /locus_tag="PZ377_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDM6358706.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            3905..4507
                     /gene="ruvA"
                     /locus_tag="PZ377_001839"
     CDS             3905..4507
                     /gene="ruvA"
                     /locus_tag="PZ377_001839"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDM6358707.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            4539..5543
                     /gene="ruvB"
                     /locus_tag="PZ377_001840"
     CDS             4539..5543
                     /gene="ruvB"
                     /locus_tag="PZ377_001840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDM6358708.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            5545..6570
                     /gene="queA"
                     /locus_tag="PZ377_001841"
     CDS             5545..6570
                     /gene="queA"
                     /locus_tag="PZ377_001841"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDM6358709.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            6593..7732
                     /gene="tgt"
                     /locus_tag="PZ377_001842"
     CDS             6593..7732
                     /gene="tgt"
                     /locus_tag="PZ377_001842"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDM6358710.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            7751..8011
                     /gene="yajC"
                     /locus_tag="PZ377_001843"
     CDS             7751..8011
                     /gene="yajC"
                     /locus_tag="PZ377_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDM6358711.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            8286..>10092
                     /gene="secDF"
                     /locus_tag="PZ377_001844"
     CDS             8286..>10092
                     /gene="secDF"
                     /locus_tag="PZ377_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDM6358712.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEPV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSV"
BASE COUNT         3531 a         1399 c         2012 g         3150 t
ORIGIN      
        1 gagaagtgac taaggttcaa aatgatcaat acggcttagc taaagaagtt agggttaaga
       61 ctggtgcgga cttaacagat ttgagtcatg tttatgttgc aaaaagagat cctaaaacaa
      121 ttcctgatga tgaaagcagg gataaataat gcgtacactg tattattttt tgataggtat
      181 tttactattt tatatagata ctgcaattgg gcttcttatt ccaatgcaca taggtaagtt
      241 tgaacttgta tttgtaccac accttacatt tatgtatatt ttaatgatgg tagtgtatcg
      301 tggctttggc gtatcattat tgctcagcat atttttaggc gtaatgactg atgtatactt
      361 tggtagtatt tatggagtgt acttatttgg ctatatattg tttttagcac ttatagatcg
      421 attctttaaa attttttaca aagatcattc gatgttattc attattatat tagccagcac
      481 cttattatta gaagtctatg tggcattaat atacggtatg ttaggattca ttcaatttga
      541 tattattcat tttgtagtct ttagattatt gccaacatta attatgaatt ttgtactgtt
      601 gattatgctt tatccgttga ttataaagtt ccttaaaaaa acaaacaatg acattgacat
      661 gaaacgtcgt caatggtaag atgcaatagt tgagtagtta taagtagcta catacaaccg
      721 ctcaaatata ggttcaagaa catttttaat gaagaaagcg attttcccac agagtgcaaa
      781 cgcttgactc aataacgaaa tgtcacctat gtatggcgtg acttattata caggaggtgc
      841 aaagtatgtt tgctattatt gaaacaggtg gaaaacaaat caaagtagaa gaaggtcaag
      901 aaatcttcgt tgaaaaatta gacgtaaacg aaggagatac ttttacattt gataaagtat
      961 tatttgtagg tggagattca gttaaagttg gagcgccaac agttgaaggt gcaacagtta
     1021 ctgctactgt taataaacaa ggtcgcggta aaaaaatcac tgtattcaca tacaaacgtc
     1081 gtaaaaattc aaaacgtaaa aaaggccatc gtcaaccata cactaaatta acaatcgata
     1141 aaatcaacgc gtaattatta tgattactgt tgatattaca gttaatgatg aaggcaaagt
     1201 aacagacgtt attatggatg gccatgctga ccatggtgaa tatggtcatg atatcgtttg
     1261 tgctggagct tcagctgtat tgtttggtag tgttaatgcg attataggat tgacatctga
     1321 gagaccagat atcaattatg acgacaatgg tggtcatttt catataagaa gcgttgatac
     1381 aaacaacgat gaagcgcaac taattcttca aacaatgctt gtgtctttac aaactattga
     1441 agaagaatat aatgagaata ttagattaaa ttataagtga ggtgcattcc gatgttaaaa
     1501 ttaaacttac aattcttcgc atctaaaaaa ggggtaagtt ctacaaaaaa cggacgtgac
     1561 tctgaatcaa aacgcttagg tgctaaacgt gctgacggtc aattcgtaac aggtggttca
     1621 attttatatc gccaacgtgg tactaaaatt taccctggtg aaaatgtagg tcgtggtggc
     1681 gatgatacat tattcgctaa aatcgacggc gttgttaaat tcgaacgtaa aggtcgcgac
     1741 aaaaaacaag tttctgtata tgcagtagct gaataatttt gtctagttaa caccagaagt
     1801 gaatcttctg gtgtttttta ctttttataa aatgtttttc attattattt tcattatgtt
     1861 attaaaaaat gggaatttta gacgttatac taaatgtgca actgtataag agcccctaat
     1921 cactaaacta taagggggac aaaggaatac agttgcagcg tttaaagaat aaactgtacc
     1981 acaattggtg ctgagaaata taagtatttt aaagcaaaga tttataaaag taactgcata
     2041 agagccccta attatttaca atatataagg ggctctaata tgctataatt attgggaaaa
     2101 tgaaaattat atgtaaaaga ggtgagatat atgtttgtcg atcaagtcaa aatatctctt
     2161 aaagccggtg atggtggtaa tggtattacc gcatacagaa gagaaaaata tgtaccattt
     2221 ggtggaccag ctggcggtga cggtggtaaa ggtgcttcag tcgtatttga agtggatgaa
     2281 ggtttaagaa cgttattaga ttttagatat caacgtcatt ttaaagcaag caaaggtgaa
     2341 aatggccaaa gtagtaatat gcatggtaaa aatgcggaag atttagtatt aaaagttcca
     2401 cctggtacaa ttattaaaaa tgttgaaaca gacgaagtgt tagcagatct tgttgaagat
     2461 ggtcaaagag ctgtagtagc gaagggcggt cgaggtggcc gaggtaattc acgttttgca
     2521 acacctagaa accctgcacc tgacttcagt gaaaaaggtg aaccaggtga ggaattagat
     2581 gtatctttag aattgaaatt attagctgat gtaggattag taggtttccc tagtgtgggt
     2641 aaatcgactt tattatctat cgtttcaaaa gctaagccta aaattggggc atatcatttt
     2701 acaacgatta aaccaaatct aggtgttgtt tcaacgcctg atcaacgtag ttttgttatg
     2761 gcagatttac caggtttaat tgaaggtgca tctgatggcg ttggattagg acatcaattt
     2821 ttaagacatg tagagagaac aaaagttatt gttcacatga ttgatatgag cggttctgaa
     2881 ggtagagaac ctattgaaga ttataaagtc attaatcaag aattagctgc gtacgagcaa
     2941 cgtttagaag atagacctca aatcgtagta gctaacaaga tggatttacc tgaatcacaa
     3001 gataatttaa acttgtttaa agaagaaatt ggcgaagatg tgccagttat tccagtttca
     3061 acaataacgc gtgataatat tgatcaatta ttatatgcaa tagcagataa attagaagaa
     3121 tataaagatg ttgacttcac agttgaagaa gaggagtcag ttggcattaa ccgagtatta
     3181 tataaacata caccgtcaca agataaattt acaatttcaa gagatgatga tggtgcttat
     3241 gtggtaagtg gtaatgctat tgaaagaatg tttaaaatga ctgactttaa cagtgatcca
     3301 gcagtacgtc gatttgctcg tcaaatgcgt tcgatgggta ttgatgatgc gcttagagaa
     3361 cgtggttgta aaaatggtga tatcgttaga attcttggcg gagaatttga attcgttgaa
     3421 taggagcgaa acatgatgga caataaagat tataaaaagt tttatttaat tagagaagat
     3481 gtcttgcctg aatccgtggt taaaacattg aagattaaag atgccttaaa aagtgatccg
     3541 acattgtcca tttatgatgc cgttaaacag tttgatctat ctagaagtgc tttttataaa
     3601 tatagagaaa cgatatttcc agtagacgat aaaatgcttg accatcgaga atttacatta
     3661 attttatatg taactgatat tgttggtatg ttggcacgtg tactagatgt tatatcaaag
     3721 ttagaactat ctgtattaac gattcatcaa agtattccaa tggaagaaaa agcaacaata
     3781 acattatcac tgaatgctaa atctaaagaa acttcagtag aagatgttat tggcgctttg
     3841 agaaatttag attatgtatc aaaagtagaa ttaattagta tgagtatgta aggatgtgcc
     3901 tataatgtac gcgtatgtca aaggtaagtt aacacattta tatcctacac acgtagttgt
     3961 tgaaactgct ggtgttggtt atgaaattca aacaccaaat tcttatcgtt ttcaaaagca
     4021 tctagatcat gaagttttaa ttcatacatc tttaattgtt cgtgaagatg cacaattatt
     4081 gtatggattt agtagtgaag aagagaaaga tatgttcttg agtttaatta aagttactgg
     4141 tattggtccg aaatcagctt tagctatttt agcgacaagt acgcctaatg aagtaaaacg
     4201 tgccattgaa aatgaaaatg atacgtattt aactaaattc ccaggaattg gtaagaaaac
     4261 ggcaagacag attgtcttag atttaaaagg taaagtgaaa attactgaag aagatagcga
     4321 ttcattatta caagtagacg ctacttcgac ggtgcaagat caattcgtgc aagaagcaat
     4381 gttagcgtta gaagcattag gttattctaa acgagagctt gcaaaagttg agaaaacgtt
     4441 aaataaaaat aaatatgact cagttgatga agctgttaag gcaggtcttc aattagttgt
     4501 atcttaattt taaatagatt aataggggaa gtgttgtcat gaatgagcgt atggttgatc
     4561 aatcaatgca tagtgaagaa actgatttcg aattgtcgct tagacctacg agattacgac
     4621 aatatattgg tcaaaattca ataaaaagta atttagaagt atttattaaa gcggctaaac
     4681 ttcgtcatga accattagat catgtattgc tttttggccc ccctggatta ggtaagacaa
     4741 cattatctaa tatcattgcc aatgaaatgg aagttaatat acgtacagta tcagggcctt
     4801 cattagaaag acctggtgat ttggctgcaa ttttatcagg acttcaacct ggagatgttt
     4861 tgtttattga tgaaatacac agactgagta gtgttgttga agaagtgtta taccctgcaa
     4921 tggaagattt ctttttagat attatcattg gtaaaggcga tgaggctaga agtatccgta
     4981 tcgacttacc tccattcact ttggtaggtg caacaacgcg agctggcagc ttaacaggtc
     5041 cactaaggga tcgatttggt gtgcacttaa gattagaata ttataacgaa tcagatttaa
     5101 aagaaatcat tattagaaca gctgaggttt taggcacagg tattgatgaa gaaagtgcca
     5161 ttgaacttgc taaacgttct agagggactc caagagtagc aaatcgacta ttgaagcggg
     5221 taagagactt ccagcaagtg aatgaagatg aacaaatata cattgaaaca acgaagcacg
     5281 cattaggttt acttcaagtt gatcaacacg gactagatta cattgatcat aaaatgatga
     5341 actgtattat taagcagtat aatggcggac ctgttggttt agatacgatt gccgtaacaa
     5401 ttggtgaaga acgtattaca attgaggacg tttatgagcc atttcttatt cagaaaggct
     5461 ttttagaacg tacgccacgt ggcagaaaag caacaccatt agcttatgaa cattttgcaa
     5521 agtcgaatga ggagagagaa taacgtgaat attgaagaat ttgactatga cttaccagaa
     5581 tcattaattg ctcaaacgcc tttaaaagat cgtgatcata gtcgtttatt agtcatggat
     5641 agagaaactg gtgaaatgaa acatttacat ttcaaagata tcattgagta ttttagacct
     5701 ggtgatacat tagtgcttaa cgatacgcga gtaatgccag ctagactttt tggtttaaaa
     5761 gaagaaactg gtgcaaaagt tgaaatgtta atgttaactc aaattgaagg taatgattgg
     5821 gaagtcttac tgaaaccagc taagcgtatt aaagttggta ataaattgaa ttttggtaat
     5881 ggcaaaatta tagctgaatg cataaaagaa atggatcaag gtggacgcat catgcgttta
     5941 cattatgaag gtattttaca agaaagatta gatgaattag gggaaatgcc actgccacca
     6001 tacatcaaag aacgtttaga tgatccagat cgttatcaaa cagtttacgc taaagaaagt
     6061 ggttcagcgg cagcaccaac agcaggatta cattttactg atgagttatt aattgaaatt
     6121 aaaaataaag gtgttaatat cgcatttgtt acattacatg ttgggttagg tacgtttaga
     6181 ccggtgagcg tagacgatgt gaatgaccac gaaatgcata gtgaatatta tcaaatgaca
     6241 caagaaacag ctgatttatt aaatgatact aagtccaaag gacatcgcat tatatcagtt
     6301 ggtacaactt caacacgtac acttgaaaca attcgacgcg atcatgataa atttgttgaa
     6361 acgagtggct ggactaatat atttatttat ccaggatttg attttaaagc aattgatggc
     6421 cagattacta attttcattt accaaaatca acattagtta tgctagtatc agcgtttagt
     6481 agtcgtgaaa atgttctgaa tgcttataaa acggcagtaa atttagaata tagattcttt
     6541 agttttggcg atgcaatgtt aattatataa aaagaatgtg aggattttga atatgcctgc
     6601 agtaacatac gaacacatta aaacttgtaa acaatcaggt gcgcgtttag gtatcgtgca
     6661 cacaccacac ggttcatttg aaacacctat gtttatgcca gttggtacta aagcaaccgt
     6721 taaaacaatg agtccagaag agttaagaca aattgaagca aaaatcattt tgggcaacac
     6781 atatcatttg tggttacaac ccggaaatga tattatcaaa cacgctgggg gattacataa
     6841 attcatgaat tgggatggtc cgattcttac agattcaggc ggtttccaag tgtttagttt
     6901 aagtaattta cgtaaaatta cagaagaagg cgtggaattt agacatcata ctaatgggtc
     6961 taaattattt ttgagtcctg agaaatcaat gcaaattcaa aatgatttag gatctgatat
     7021 tatgatggca tttgatgaat gtccaccgat gcctgctgaa tatgattatg taaaaaaatc
     7081 tattgaacgt acaacacgtt gggcgaaaag atgtctagat gcacaccaaa gacctgaaga
     7141 tcaagcattg ttcggcatta tacaaggtgg cgaatatgaa gatttaagag aacaaagtgc
     7201 aaaggattta gtagaattag attttcctgg ttatgcaatc ggcggtttgt cagttggtga
     7261 acctaaaccg gttatgtata aaatggttga acatacagag cagtttatgc ctaaagataa
     7321 accaagatat ttaatgggtg taggatctcc agatgcgtta atcgaatgta gtattcgcgg
     7381 catggatatg tttgattgtg tcttaccgac acgtattgcc agaaatggta cttgtatgac
     7441 atcgcaaggt cgtttagtta ttaaaaatgc aaaatttgca gatgatttaa gaccgttaga
     7501 tgagaattgt gactgttata catgtcaaaa ctattcaaga gcgtatatac gtcatttaat
     7561 caaggcagag gaaacttttg gtattcgtct tactactatt cataatttac attttctgct
     7621 aaaattaatg gaagatataa gacaagccat tcgagaagat cgtcttttag atttcaaaga
     7681 agaattcttc gagcaatatg gattaaatgt tgagaaccca aaaaactttt aagcaagagg
     7741 agcgtataaa atgcaatttt cattactaat atatatagtc gtaatttttg cggttatgta
     7801 tttcttgatg atcagaccac aacaaaaacg tgcgaaacag catcgtgagt tgattaataa
     7861 cattcaatct ggtcaaagaa ttacaactat tggtggtatt aaaggtactg ttaaagcagt
     7921 agatgaaaca actgttgtta ttacagttaa tggtcatggt actgaattaa ctttcgaaaa
     7981 acctgctatt aaacaagttg acccttcata atgagaagtg gtattaaaaa ggatgaaacg
     8041 aatagcgttt catccttttt aagttataag tagtatgtag aatagcttat ttttattcca
     8101 aaaaaagaaa ttgtgataca taataattgt taaatttaaa ttaaatatat ctatgactat
     8161 ttttactccc tgctctagta tgttaatatg tataaggcta ataaattttg taagaaagat
     8221 ttaacttgcg ttataaaata gtattcaagt attctaaata catacgtaaa taatgaggtg
     8281 ttcatgtgaa gaaaagtagt agaataattg cgttcttgct tttagtcgta ttgttgtttg
     8341 ccggaatggc tgcaacttat aaaagtgtta taaagaatgt caatttagga cttgatttgc
     8401 aaggtggatt tgaggtgctt tatcaagtcg atcctttaaa taaaggcgac aaaatagata
     8461 aaaaagcctt acaatcaaca gcgcaaacat tagaaaatcg tgttaacgtt ttaggtgttt
     8521 cggaacctaa aattcaagtt gaagaaccta atagaattag agtacaactt gctggtgtaa
     8581 cagaccagaa tgaagcacgt aaaattttat cttctcaagc taatttaaca attcgcgatg
     8641 cagaagataa agtaaaatta agcggttctg atattaaaca gggatctgcc aaacaagaat
     8701 ttaaacagga aacaaaccag cctacagtta cttttaaagt aaaagacaaa aacaaattta
     8761 aaaaagtaac ggaagaaatc tctaagaaac gtgataatgt catggttgtt tggttagatt
     8821 tcaaaaaagg tgacagctat aaaaaagaag cacaaaagaa aaaccctaaa tttatttctg
     8881 cagcaagtgt tgatcaacca atcaattctg atagcgtaga aatctcgggt ggatttaaag
     8941 gacaagaagg cgttaaaaaa gcaaaacaaa tcgccgaatt gttaaatgca ggttctttac
     9001 cagttgactt gaaagaaatt tattcaaact cagtaggtgc ccaatttggt caagatgcat
     9061 tagataaaac tgtgtttgca tcgtttattg gtgtagcatt aatttattta ttcatgcttg
     9121 gattctaccg attacctggt ttagtagcga ttattgcatt gacaacttat atctatctaa
     9181 cgttagtagc atttaatttc atttccgggg ttctaacttt accaggatta gcggcgttgg
     9241 tattaggtgt aggtatggct gtagatgcca atattatcat gtatgagcgt attaaggatg
     9301 aacttcgaat aggtagaacg ataaagcaag ccttttctaa agcaaacaaa agttcattcc
     9361 taacaatttt tgattctaac ttaacaacag ttatcgccgc agcagtatta ttcttcttcg
     9421 gtgaaagttc agttaaaggt ttcgcgacaa tgttattatt aggtattcta atgatctttg
     9481 ttacagccgt gttcttatca agattcttat tatcattact tgtttcatca aatatattca
     9541 aaaatcaatt ttggttattt ggtgttaaaa agaataaacg acatgatatt aatgaaggtg
     9601 tagatgttca tgaccttaaa acttcattcg agaaatggaa ttttgttaaa ttagctaagc
     9661 cattaattgg agtaagtatt ttaattgtag ttgtcggttt agttattctt tatatcttca
     9721 agttaaactt aggaattgat ttctcaagtg gtacacgcgt agatttccaa tctaaacaag
     9781 caattactca acaaaaggta gagccagttg taaaagatag tggacttaaa gcggatcaaa
     9841 ttcagatcaa tggtaaagac aataaagtag caactgttca atttaaagac gatttaacgc
     9901 gtgctcaaga taataaattg agtgataata ttaaatcgaa attcggtgac acacctcaaa
     9961 ttaacactgt atcaccaatt attggacagg aattagcaaa aaatgcaatg cttgcattaa
    10021 tctatgcatc gataggtatc atcatctatg tatcattacg atttgaatgg cgcatgggtc
    10081 tttcatctgt at
//
LOCUS       DANRDI010000097        10031 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.097, whole genome shotgun sequence.
ACCESSION   DANRDI010000097 DANRDI010000000
VERSION     DANRDI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.097"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..594
                     /locus_tag="PZ377_001845"
     CDS             <1..594
                     /locus_tag="PZ377_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HDM6358713.1"
                     /translation="SPESFVSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKE
                     CSEHRMLVDLGRNDIHRVSKIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTV
                     IANLLPTGTVSGAPKLRAIERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMID
                     EQYINVEAGCGVVYDSIPEKELNETKLKAKSLLEVSP"
     gene            591..1157
                     /locus_tag="PZ377_001846"
     CDS             591..1157
                     /locus_tag="PZ377_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDM6358714.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            1163..2161
                     /gene="trpD"
                     /locus_tag="PZ377_001847"
     CDS             1163..2161
                     /gene="trpD"
                     /locus_tag="PZ377_001847"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDM6358715.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            2163..2945
                     /gene="trpC"
                     /locus_tag="PZ377_001848"
     CDS             2163..2945
                     /gene="trpC"
                     /locus_tag="PZ377_001848"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDM6358716.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            2945..3577
                     /locus_tag="PZ377_001849"
     CDS             2945..3577
                     /locus_tag="PZ377_001849"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDM6358717.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            3570..4784
                     /gene="trpB"
                     /locus_tag="PZ377_001850"
     CDS             3570..4784
                     /gene="trpB"
                     /locus_tag="PZ377_001850"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDM6358718.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            4777..5505
                     /locus_tag="PZ377_001851"
     CDS             4777..5505
                     /locus_tag="PZ377_001851"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDM6358719.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            5827..7089
                     /gene="femA"
                     /locus_tag="PZ377_001852"
     CDS             5827..7089
                     /gene="femA"
                     /locus_tag="PZ377_001852"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDM6358720.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            7108..8367
                     /gene="femB"
                     /locus_tag="PZ377_001853"
     CDS             7108..8367
                     /gene="femB"
                     /locus_tag="PZ377_001853"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDM6358721.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            9056..9823
                     /locus_tag="PZ377_001854"
     CDS             9056..9823
                     /locus_tag="PZ377_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDM6358722.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(9871..>10031)
                     /locus_tag="PZ377_001855"
     CDS             complement(9871..>10031)
                     /locus_tag="PZ377_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDM6358723.1"
                     /translation="RNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEE
                     EMFEDEFY"
BASE COUNT         3672 a         1456 c         1816 g         3087 t
ORIGIN      
        1 tctcccgaaa gtttcgtaag tgtcaaagat caaattgtaa caactaatcc tattgcaggt
       61 acgattcaac gtggtgagac gacacaaata gataatgaga atatgaaaca actacttaat
      121 gatccaaaag aatgcagcga acatcgtatg ctagttgatt taggacgtaa tgatattcat
      181 agagtaagta aaatcggtac ctcaaaaatt actgaattaa tggttattga aaaatatgaa
      241 catgttatgc atatcgtaag tgaagtcaca ggtaaaataa atcaaaattt atcgccaatg
      301 acagttattg cgaatttatt accaacaggt accgtttcag gtgcaccaaa attacgtgca
      361 attgaaagaa tatatgaaca atatccacat aaacggggcg tttatagtgg tggtgttgga
      421 tacataaatt gtaatcataa cttagatttt gcattagcaa ttcgaacgat gatgatagat
      481 gagcagtata tcaacgtaga agctggttgt ggcgttgtat atgattctat tcctgaaaaa
      541 gaactgaatg aaacgaaatt gaaagctaaa agcttattgg aggtgagccc atgatcttag
      601 ttgtagataa ttatgattcc tttacatata acctagtgga tattgttgct caacatactg
      661 acgtcattgt tcaataccct gatgatgata atgtgctgaa tcaatcggtg gacgctgtta
      721 ttatatctcc tggtccaggg catccattag acgatcaaca gttaatgaaa atcatatcaa
      781 cctatcaaca caaacccatt ttaggtattt gtttaggggc tcaggcactg acttgttact
      841 acggtggaga agtcattaaa ggcgacaagg ttatgcacgg caaagttgat acactaaagg
      901 ttatatcgca tcatcaacat ctgttatatc aagatatacc agaacagttt tcaattatga
      961 gatatcattc attaataagt aaccctgaca attttccaga agaattgaaa attactggac
     1021 gtaccaaaga ttgtatacag tcattcgagc ataaagaaag accgcattat ggtattcagt
     1081 accatcctga atcatttgct acagactatg gtgtcaaaat aattacaaat ttcattaatc
     1141 tagtgaagga aggatgaaaa ccatgacatt actaacaaga ataaaaactg aaactatatt
     1201 acttgaaagc gacattaaag agctaatcga tatacttatt tctcctagta ttggaactga
     1261 tattaaatat gaattactta gttcctattc ggagcgagaa atccaacaac aagaattaac
     1321 atatattgta cgtagcttaa ttaatacaat gtatccacat caaccatgtt atgaaggggc
     1381 tatgtgtgtg tgcggcacag gtggtgacaa gtcaaatagt ttcaacattt caacgactgt
     1441 tgcttttgtt gtagcaagtg ctggcgtaaa agttataaaa catggtaata aaagtattac
     1501 ctcaaattca ggtagtacgg atttgttaaa tcaaatgaac atacaaacaa caactgttga
     1561 tgatacacct aaccaattaa atgaaaaaga ccttgtattc attggtgcaa ctgaatcata
     1621 tccaatcatg aagtatatgc aaccagttag aaaaatgatt ggaaagccta caatattaaa
     1681 ccttgtgggt ccattaatta atccatatca cttaacgtat caaatggtag gcgtctttga
     1741 tcctacaaag ttaaagttag ttgctaaaac gattaaagat ttaggtagaa aacgtgcaat
     1801 cgttttacat ggtgcaaatg gtatggatga agcaacacta tctggtgata atttgatata
     1861 tgaattgact gaagatggag aaatcaaaaa ttacacatta aatgcgactg attatggttt
     1921 gaaacatgcg ccgaatagtg attttaaagg cggttcacct gaagaaaatt tagcaatctc
     1981 ccttaatatc ttgaatggta aagatcagtc aagtcgacgt gatgttgtct tactaaatgc
     2041 gggtttaagc ctttatgttg cagagaaagt ggataccatc gcagaaggca tagaacttgc
     2101 aactacattg attgataatg gtgaagcatt ggaaaaatac catcaaatga gaggtgaata
     2161 atatgacgat tttatcagaa attgttaaat ataaacagtc acttttacaa aatggctatt
     2221 atcaagacaa acttaatacc ttgaaaagtg tgaagattca gaataaaaaa tcttttataa
     2281 acgcaattga gaaagaacca aagctagcaa ttattgcaga aattaaatcg aagagtccta
     2341 cagttaatga cttacctgaa cgagatttat cgcaacaaat ctcagattat gaccaatatg
     2401 gtgcaaatgc cgtgtccatt ttaactgatg aaaagtactt tggtggtagt tttgaaagat
     2461 tacaagcatt gacgacaaaa acaacattac ccgtattatg caaagacttt attatagacc
     2521 cgcttcaaat tgatgttgct aaacaagctg gtgcatctat gattttattg atcgttaaca
     2581 tcttatctga taaacaattg aaagatttat ataactacgc tatatcgcaa aatctagaag
     2641 tgttagttga agtacatgat cgccatgaat tagaacgtgc ctataaggtt aatgctaaat
     2701 tgattggtgt aaataacagg gacttaaaac gatttgttac aaatgtggaa catacaaata
     2761 ctattttaga aaataaaaaa acaaatcatt attatatttc tgaaagtggt attcacgatg
     2821 catctgatgt aagaaaaatc ttgcatagtg gtatcgatgg cttactaata ggtgaggcgc
     2881 ttatgcgttg tgacaatcta tctgaatttt taccacaact gaaaatgcaa aaggtgaagt
     2941 catgatgaaa ttgaaatttt gtggctttac atcaataaag gatgttacag cggccagtca
     3001 attacctatt gatgcgatag gtttcatcca ttatgaaaaa agtaaaaggc atcaaacaat
     3061 tacccaaata aaaaagttag cgtctgctgt tccaaatcat atcgataaag tatgtgtcat
     3121 ggtaaatcct gatttaacaa caattgaaca cgtattaagc aatacgtcaa ttaacacaat
     3181 acagttacac ggcacagaat ctattgattt tatacaggaa attaaaaaga aatattcaag
     3241 cattaaaatc actaaagctt tagctgcaga tgaaaacata atccaaaaca taaataaata
     3301 taaagggttc gtagatttat ttattatcga cacaccctca gtgtcgtatg gtggtaccgg
     3361 tcaaacatat gactggacta ttttgaagca cataaaagac ataccttatt tgatagcagg
     3421 aggcattaac tctgaaaata ttcaaacagt taatcaactt aaattatcac atcaaggtta
     3481 tgatcttgca tcaggtatag aagtaaatgg gcgaaaagat atagaaaaaa tgacagcaat
     3541 tgtaaatatt gtgaaaggag atagagacaa tgaataaaca aatacaaaca gaagcagatg
     3601 aattaggttt ctttggtgaa tatggagggc aatatgttcc agaaacatta atgccagcaa
     3661 ttattgagtt gaaaaaagct tataaagagg caaaagcaga cccagagttt caaagagaac
     3721 tggaatacta tttatcagag tatgtaggac gcgcgacacc acttacatat gctgcatcat
     3781 atactgaaag cctaggtggc gctaaaatat atttgaaacg agaggatcta aatcatacag
     3841 gcgcccataa aattaataat gcgttaggtc aagcgttgct tgctaaaaga atgggcaaga
     3901 agaagcttgt tgctgaaact ggtgcgggtc aacatggtgt agctagtgct acggttgctg
     3961 cattatttga tatggaactt gttgtcttta tgggaagtga agatattaaa agacaacaac
     4021 ttaatgtatt tagaatggaa ttacttggtg caaaggttgt ggcagttgaa gatggtcaag
     4081 ggactttatc ggatgcagtt aataaagcat tgcaatattg ggtaagtcat gtagatgata
     4141 cacattattt attaggttct gcattaggtc cagacccgtt cccaacgatt gttagagatt
     4201 ttcagagtgt gattggtaaa gaaataaaat cacagatatt gaagaaagaa ggtcgacttc
     4261 cggatgcaat tgtagcatgt atcggtggtg gctcaaatgc aatcggtaca ttttatccat
     4321 ttattaaaga tgatgttgca ttatacggtg ttgaagccgc aggtcaaggc gatgatactg
     4381 ataaacatgc acttgcaatt ggcaaaggat cacctggcgt attacatggt actaaaatgt
     4441 atttaattca agatgaagat gggcaagtgc aactagcaca ttctatttca gcaggacttg
     4501 attatcctgg tattggacca gaacattctt attaccacga cattggtaga gtaacttttg
     4561 aaaatgctag tgatacacaa gcaatgaatg ctttaatcaa ctttacaaaa catgaaggta
     4621 ttatacctgc aattgaaagt gcacatgcac tgagttatgt tgaaagacta gcgcctacga
     4681 tgtcgaaaga agatattatt gtagtaacta tttctggacg tggcgataaa gatatggaaa
     4741 caattagaca atatatggta gagcgaggtc ttgcaaatga ctaaattatt tataccttat
     4801 attatgggca ataaagattt gattgaaaat gcaacattgt tgagtgaaaa tggtgcagat
     4861 ataattgaaa ttggagtacc tttctctgat ccggttgctg atggtccagt tatcatggaa
     4921 gcaggtcaac aagcgattaa acaaggcatc acgatagatt atattttcaa tcaattagaa
     4981 aaacatggtg atcaaattaa gtgtaactat gtattaatga cgtattataa tattatttgt
     5041 cattatggag aacaagcgtt ttttgaaaaa tgtcgagata ctggtgtcta cggcttaatt
     5101 attcctgatt taccatatga attatcgcag cgtttaaaac aacaatttag tcactatggc
     5161 gtcaaaatca tatcgttagt tgcgatgact actgatgaca aacgtataaa agatatcgta
     5221 tcccatgcgg aaggctttat ttatactgtg acgatgaatg cgacaacagg gcaaaacggt
     5281 gcgtttcatc cagaattaaa acgaaaaatt gagtcaatta aagcgatagc caatgtgcca
     5341 gttgtcgcag gatttggtat aagaacacca caacatgttg cagatataaa agaggttgca
     5401 gatggcattg tcattggtag cgaaatcgtt aagcgattta aatctaacac gcgtgaggaa
     5461 atcattaaat atttacaatc tatccaacaa acattgaata attaagttta cttgatttaa
     5521 aaaaattagg cgaatactgt ttgaaaaagt gaaaaacggt gaattataaa attgaataga
     5581 aatgatacaa aagtaatatg agcaaaccca aacgttcata ttactttttt tgaaattgta
     5641 ttcaaaaatc taaatattac tataaaagta tacgcaatta aagcgtttat gttttagttt
     5701 taacattaac tattgtatac ttatttagat tagatttatt atttttgaca tttgcagagg
     5761 ggaaatagaa aaactgcaaa tacggaaatg aaattaatta acgagagaca aataggagta
     5821 atgataatga agtttacaaa tttaacagct aaagagtttg gtgcctttac agatagcatg
     5881 ccatacagtc atttcacgca aactgttggc cactatgagt taaagcttgc tgaaggttat
     5941 gaaacacatt tagtgggaat aaaaaacaat aataacgagg tcattgcagc ttgcttactt
     6001 actgctgtac ctgttatgaa agtgttcaag tatttttatt caaatcgcgg tccagtgatt
     6061 gattatgaaa atcaagaact cgtacacttt ttctttaatg aattatcaaa atatgttaaa
     6121 aaacatcgtt gtctatacct acatatcgat ccatatttac catatcaata cttgaatcat
     6181 gatggcgaga ttacaggtaa tgctggtaat gattggttct ttgataaaat gagtaactta
     6241 ggatttgaac atactggatt ccataaagga tttgatcctg tgctacaaat tcgttatcac
     6301 tcagtgttag atttaaaaga taaaacagca gatgacatca ttaaaaatat ggatggactt
     6361 agaaaaagaa acacgaaaaa agttaaaaag aatggtgtta aagtaagatt tttatctgaa
     6421 gaagaactac caatttttag atcatttatg gaagatacgt cagaatcaaa agcttttgct
     6481 gatcgtgatg acaaatttta ctacaatcgc ttaaaatatt acaaagaccg tgtgttagta
     6541 cctttagcgt atatcaactt tgatgaatat attaaagaac taaacgaaga gcgtgatatt
     6601 ttaaataaag atttaaataa agcgttaaag gatattgaaa aacgtcctga aaataaaaaa
     6661 gcacataaca agcgagataa cttacaacaa caacttgatg caaatgagca aaagattgaa
     6721 gaaggtaaac gtctacaaga agaacatggt aatgaattac ctatctctgc tggtttcttc
     6781 tttatcaatc catttgaagt tgtttattat gctggtggta catcaaatgc attccgtcat
     6841 tttgccggaa gttatgcagt gcaatgggaa atgattaatt atgcattaaa tcatggcatt
     6901 gaccgttata atttctatgg tgttagtggt aaatttacag aagatgctga agatgctggt
     6961 gtagttaaat tcaaaaaagg ttacaatgct gaaattattg aatatgttgg tgactttatt
     7021 aaaccaatta ataaacctgt ttacgcagca tataccgcac ttaaaaaagt taaagacaga
     7081 attttttagg aagggaatta tcaaaacatg aaatttacag agttaactgt taccgaattt
     7141 gacaactttg tacaaaatcc atcattggaa agtcattatt tccaagtaaa agaaaatata
     7201 gttacccgtg agaatgatgg ctttgaagta gttttattag gtattaaaga cgacaataac
     7261 aaagtaattg cagcaagcct tttctctaaa attcctacta tgggaagtta tgtttactat
     7321 tcgaatcgtg gtccagtaat ggatttttca gatttaggat tagttgatta ttatttaaaa
     7381 gagttagata aatatttaca gcaacatcaa tgtttatatg ttaaattaga tccgtattgg
     7441 ttatatcatc tatatgataa agatatcgtg ccatttgaag gtcgcgagaa aaatgatgcc
     7501 ctagtaaact tgtttaaatc acatggttac gagcatcatg gctttacaac tgagtatgat
     7561 acatcgagcc aagtacgatg gatgggcgta ttaaaccttg aaggtaaaac acccgaaaca
     7621 ttgaaaaaga catttgatag tcaacgtaaa cgtaatatta ataaagcgat aaactatggt
     7681 gttaaagtca gattccttga acgtgatgag ttcaatcttt tcttagattt atatcgtgaa
     7741 actgaagagc gtgctggatt tgtatcaaaa acagatgatt atttttataa ctttattgac
     7801 acatatggag ataaagtatt agtaccatta gcatatattg accttgatga atatgtgtta
     7861 aagttgcaac aggaattgaa tgacaaagaa aatcgtcgtg atcaaatgat ggcgaaagaa
     7921 aacaaatcag ataagcaaat gaagaaaatt gcagaattag ataagcaaat tgatcatgat
     7981 cagcatgaat tattgaatgc aagtgaattg agcaaaacgg acggcccaat tctaaacctt
     8041 gcttctggcg tttattttgc aaatgcatat gaagtgaatt atttctctgg tggttcatca
     8101 gaaaaatata atcaatttat gggaccatac atgatgcatt ggtttatgat taactattgc
     8161 ttcgataatg gctatgatcg ttataatttc tatggtttat caggtgattt tacggaaaac
     8221 agtgaagatt atggcgtata ccgctttaaa cgtggattta atgtacaaat cgaagaatta
     8281 ataggggatt tctataaacc aattcataaa gtgaaatatt ggttgttcac aacattggat
     8341 aaattacgta aaaaattaaa gaaatagatg taaatgatgt tagggcttat agttattgat
     8401 actataggct cttttttata tgttttttaa taaagcctcg aataatgata tattaaatga
     8461 caagtttaac aagtgattgc aataaatata aatatgaatt gcacaaccta tttgtaaatg
     8521 attgagttga gtatactttt taattcattc aatatgactc aaaacaagcc aatatgtatt
     8581 atatttttgg tgaataaacc ttagcataac aatttatgtc tgattatata tgtgtgtgtt
     8641 tgcaagtgtg tgatatcaaa tgtcatattt gtctaattgt aatttataaa atgcatgagc
     8701 aatgattgct gtaacaaatt ctagcaaagt attcagtttg tattttagaa ataaaaatga
     8761 tgatagatgt atgatcgata taaaagattt aatatgcacc aaaatgataa cgtgagtaat
     8821 tggtttaatg aaacataaca tgaatgtaaa tttagcttac tgacataaac actacacaaa
     8881 catacagtta tataagtccc cttatggaat ttcacattct agtttacata atatatatta
     8941 taggaagtta tatgtgtgta acgcaaaagg taccctacat cataatcatt atctaatatc
     9001 gtcacataac ttacttatgc tataatcatg gtattatatt gtttggagtg atttgatgag
     9061 atttgtcttt gatattgatg gtacgctttg ttttgacggc cgattaattg accagactat
     9121 tattgataca ttgttacaat tacaacatga tggtcatgaa cttatatttg catcagcacg
     9181 tccgattcgt gatttgttgc cagttttacc atcagtattt catcagcaca cattaattgg
     9241 cgcaaatggt gctatgattt cacagcaatc aaagatttct gttatcaaac caattcatac
     9301 tgatacatat catcatatct tcaaaataat tcaaaagtat gagttagatt atattattga
     9361 tgatgattgg aattatgctg cacaacttga cgctgagaac gcgatttttg agcgtttaga
     9421 tccacataag ctggccagtt gtattgatgt tgcaaatatc gacacaccaa tcaagattat
     9481 tttattaaat atagacccgg cacaaattac aactatatta gacgagctag ataaatacca
     9541 tcaagaattg gaaatgattc accattcaaa tgagtataac attgatataa cagcgcaaaa
     9601 tattaacaaa tatactgcat tacaatatat atttgatgca gatgttaaat atatagcatt
     9661 tggtaatgac cacaatgata ttgtcatgtt acaacatgct agtagtggct atattatagg
     9721 accatcagaa gcatacacac acgcaatatt gaaacttgat aaaatcaaac acatcaataa
     9781 taatgcacaa gctatttgca aagtcttaaa atcatataaa taaaaacacc cctatcaaat
     9841 gataatcatt atcaatcgat aggggctatt ttaataaaat tcgtcctcga acatttcttc
     9901 ctcttcatct aatccaaata attctgccat ttctccatgt tcaattaaca tgtttaaata
     9961 tgcatcgcgg agttcttctt cactcatatc attaatcatt tctttaagac tatcaatcca
    10021 catatttctg c
//
LOCUS       DANRDI010000098        10007 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.098, whole genome shotgun sequence.
ACCESSION   DANRDI010000098 DANRDI010000000
VERSION     DANRDI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.098"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..106)
                     /locus_tag="PZ377_001856"
     CDS             complement(<1..106)
                     /locus_tag="PZ377_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDM6358724.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSE"
     gene            complement(428..1363)
                     /locus_tag="PZ377_001857"
     CDS             complement(428..1363)
                     /locus_tag="PZ377_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDM6358725.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            complement(1589..2989)
                     /locus_tag="PZ377_001858"
     CDS             complement(1589..2989)
                     /locus_tag="PZ377_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6358726.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            complement(3283..3978)
                     /locus_tag="PZ377_001859"
     CDS             complement(3283..3978)
                     /locus_tag="PZ377_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358727.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            complement(3978..4919)
                     /locus_tag="PZ377_001860"
     CDS             complement(3978..4919)
                     /locus_tag="PZ377_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDM6358728.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            complement(4936..5571)
                     /locus_tag="PZ377_001861"
     CDS             complement(4936..5571)
                     /locus_tag="PZ377_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358729.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            complement(5568..6794)
                     /locus_tag="PZ377_001862"
     CDS             complement(5568..6794)
                     /locus_tag="PZ377_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358730.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            complement(7121..7240)
                     /locus_tag="PZ377_001863"
     CDS             complement(7121..7240)
                     /locus_tag="PZ377_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358731.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            complement(7320..7460)
                     /locus_tag="PZ377_001864"
     CDS             complement(7320..7460)
                     /locus_tag="PZ377_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358732.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            complement(7464..8063)
                     /locus_tag="PZ377_001865"
     CDS             complement(7464..8063)
                     /locus_tag="PZ377_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDM6358733.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            8243..9880
                     /locus_tag="PZ377_001866"
     CDS             8243..9880
                     /locus_tag="PZ377_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDM6358734.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
BASE COUNT         3221 a         1988 c         1436 g         3362 t
ORIGIN      
        1 tctcagatgt agtaaaacca gcaacctcat gcccctcttc ttttagtctt tgaactaatt
       61 taatgccaat aaggcccgtt gcaccagtta caaaaatttt actcattata aacacctttt
      121 ctctatttgt ctttttaata taattacttg cttgatgagt ttacaaaatt cacgtgagac
      181 ttccaaatga tttgcctcaa aatttttcaa agtgtcgtcg taaaaactgt ctagtaaata
      241 ctaatagtat gtcgtagacc tatgacaaat ctgaattatg acgaagatca atcaagaaaa
      301 acagcaccaa gatcatttca atttgaaagt accttactgc tgttctttat ttattacatt
      361 tcaatcttaa taatgaccgt aatatcacgt gattgtcatt tccttgtaat catacctaaa
      421 acaccaatta atcctgtgcg tgtcgctgac tttctttagt atgaattaaa tcagtaataa
      481 agcgattgac tggcgcttca atatgacgtt gtttacctaa tgttgcaact gcgccattaa
      541 tataatcaat ttcagttttt ctattattaa caattaaatc ttgatacatg gatggataat
      601 gcgcaccaac tttttcattt aaatcaacta aatattcaaa tacttcatca acatttaaat
      661 gaacattatc aatcgtcgct acatgcacta tttcttgcgt taatttataa atcaaacact
      721 tcgcataact actttcattc agcgatgcca tattacactc caacactgtg cttaatgcat
      781 ttgccgtacc attaacacaa atctttttcc aaatcgattg gtataaatct ttactaatga
      841 caccattcaa ttccgcttcg ttaagtaaat cagcaacttt tataacattt tctttacctt
      901 catccactag ttcacctatt tcaactggtc cactaccaag taaatgactg tgtccagggc
      961 tttcaagacc tgccgtccaa gtcgtaacac ctctgacaat ttgtgattga gcaacatact
     1021 gcgcaatgac ttcttcatgc ttcagaccat tcatcgtaca tacgacgatc gtttcattat
     1081 caatatgtgg cttcatatct tccatcactt cttttaattg catagacttt ggaaatagaa
     1141 agacaacatc gtaaatgctt tcgtccggtt gatcattaaa atgatacatc ggaatgttta
     1201 actcgaatgc ctctccatta atcgttatat ttaatccatg ttgcttaacc gcttcaacat
     1261 gagatgtata tccgtcaata agtgtgacat catatcctgc ttgaaatagt ttggcaccaa
     1321 agccactacc taatgcaccc gatccagcaa ttgcaatttt catcctataa acccctcttc
     1381 atcgttgttg ttcattttaa aaatactact ttcgcatatt agcgtcaatt tgaaaatcat
     1441 tcgcttaata tgcacacagc ttttatcgtc tataaaagaa cacacctaac attaaacgtc
     1501 aatacgcaac attaagtgtg tatgataaac ttactttcac aaaatttcct ttaaaatccc
     1561 tacataataa cttgctttca tttcagtttt acaaattcgt ttgattttta ggaactaata
     1621 gtaaaatgac taaaaatgca acaattgcta gcaaggcatt aaacatcata cctgtgaaac
     1681 cacctaagtt caaatttaaa ttagctgcta acacagtata aaccgtacca gatactgcta
     1741 ctccaaatgc atttcctaat gatgacgcca ttttatacac acctgaagca acacccgact
     1801 tatcatctgg cgcactagca actgctgtat cagttgatgg tgtagcatat aatcctaaac
     1861 cagtaccaaa caataaatat ccaactatac tagatatgat ataccacact tctggtaaaa
     1921 atgttaacga caataagatt aacccaatca ctgtaaagcc acttcctagt aatagtgggc
     1981 gcttcggacc atgttgagat aaaatctttt cacctacacg aatcattgac aacactgtta
     2041 ttaaatacgt taatgaaata taacccgttt gcgaagaatt aaatcctaat tgttgttgat
     2101 aatacgtgtt aataacgata agtgcaccac ctgctacacc atttaataag aagtttgaaa
     2161 tagttgcacc actgtatcct ctatttttaa aaattgaaaa atcaacaagt ggatgcttta
     2221 ttttattttc gtaatataca aaaccaacta atgaacagat aaacacaaca attaaaccta
     2281 gaattaacgg tgaaactaaa ccaaaatgag acgtctgtgt gatgattaca tttaaactta
     2341 acatcgtcac tactaaaatg actaaaccaa taacgtcaaa ctttttagct tctgctttca
     2401 tacctttgat tggttctgct ttagtctcag gtgcatgttt gattaagtac attgctaata
     2461 atgttaatag aattgaaaca acaaatattg aacgccaacc tatatatgta gccattaagc
     2521 caccaaacaa cgtacaaata ccactaccac cccaagaacc aatagaccaa tagcttaagg
     2581 cacgttgtct tcttgtacca atataatatt cgttaataat agcaagtgtt gatggcataa
     2641 tacatgctgc agacaaacct tgaattattc tacctataat taaaaatgct ggcaaaggtg
     2701 taatgatgat gagtaatgaa cctacaacat ttaatatcaa tcctacataa gtaattttga
     2761 cgcgaccaaa tttatcagca acatcaccag cacctacgat aaacaaacca gcaaataagg
     2821 cagataagct aacagcgata tttatcgttc caacgtcact actatatgtt gattgtaatg
     2881 ggacaacaag attaacaagt gactgcgcga atagccaaaa ggtaataacc cctaaaacga
     2941 tacccaaaag caatcggttg tcacctctaa attgttttga tgtgtccatg aattagataa
     3001 gcctcctatg tttaagaaaa acaaattttt aatatgatat attaagaaac aatatcataa
     3061 atttgcagaa aattataaat aaaacatctc attttttagt atttttacaa taattaacgc
     3121 tttttatcca aaaactttca tgataataat tgctatttta gtgtaaacgc atacaaacac
     3181 aacctataaa aaggctactc aaagattatt tgagctcctt gagtagcctc attttttata
     3241 aatcatgaaa cgaaatcgct aaattataaa catcttctac tattacatat taatactttg
     3301 cggccgatga ttagattgat ttttacgatg tcgtgttgtt gggtctaatc gtttttctaa
     3361 aaatcttaat agtacatcaa tgacgattgc aatgatagca atcggaatcg cacctgctaa
     3421 aataaacgtt gtgccatccg tcgcatttgt accacgaatc acaatgtcac caagcgtagg
     3481 tgctcctata aatgatccaa cggcaacaac acctatcgca acaaccaagg caatacgaat
     3541 gccaccgata ataaccgaaa cagataacgg taattcaatc attcgtagca cttgattgcg
     3601 tgtcattccc atacctttgc cagcatcctt aatattcgca tcaacactag ctataccagt
     3661 ataagtgttt tttataattg gaagtaacgc atataaaaac actgttaaaa ctactgtttc
     3721 tgaacctaag cccatgacta acattaaaat agctaacatt gcaatgactg gaactgtttg
     3781 aattatattt gcaattgtaa ttacaaatcc agaaagtttt gtgtatcttg caagcaagat
     3841 tcccaatgga ataccaatta aagctgcaaa cagcacacca tagacagaca ttaataagtg
     3901 tttgaaaaat aaatcccata gataaccaaa gttcgtaaca taataattga ataattgctg
     3961 taataaatta ccttccatta cttatgacca cctttctgtt tatcaaaata gtggtgtttt
     4021 ttcaaaaatt cttctgcgac gacagcaggt tctttacctt taccatccgc ttcataattc
     4081 aagcgttgca tctctgaagt cgaaatcttt cctgtcaact tattaatcgt cgttttaagt
     4141 tctgggtgtt gccgtaataa ttcatttgtt gcaacagcac tcgcagcata aggtgggaaa
     4201 aattgtttat catctttaag tactttcaaa tcatacgccg caattcgacc atctgtagaa
     4261 taacctaatg caacgtctaa cttctctgag tttaatgcgt cgtagactag acctatttgc
     4321 attggtctca ctgtaccaaa gtcaaaacca tactcttttt taaatccttc atagccatcg
     4381 cctttacgat tcatccatga actatccata cctaaacgta aatctttact atgctttgct
     4441 aaatctgaaa ctgtctctaa atgatatttt ttagcggttt ccttcgttac catgaatgca
     4501 tacgtattcg caaaaccata cgaatcaaaa aacgtttgat caaatttctt tttaaatcct
     4561 tgttgtgttg ctatcattgc tttcttagga tttttaattg gtgcttcctt caaagctccc
     4621 gttaaatctg tgccattata tctaacacct gatatattag catccccatt aattaaggca
     4681 ttatgttgaa tcgtacttga ccctaaatta tttactaatg ttggctttat cttaccgtgt
     4741 gtatcatgct ctattaacaa ccgtaacata tgtgaaataa tttgcgattc gcttgttgat
     4801 aatgctgtaa ttttgacatc atttttcgtg ctcttactac ctagtccggg caaactacat
     4861 ccagataata cggtaagcga taagacaaac acgacaagta tatatttaat tttcttcatt
     4921 atcttagcct cctaattatc cagatacttt taagcctttg ggaactaccc atttttcaac
     4981 taaagctaat aaggcatcaa cacctaatgc tagtgcagta acgagtaccg ttgcagttac
     5041 aatcatcagt ggatcatata aatttaaacc attgaaaatg aaatcaccta atccacccgc
     5101 acctacataa cttgcaagtg tagcccaact aattacatac acagatgaca aacgaatgcc
     5161 accaatgata agcggcaatg ctaacggcaa ttcaacatcc ttcatcaatt gaaattgtgt
     5221 cattcccata ctttttccag cttctttaat gttgctatca atattttgaa caccgagtac
     5281 cgtgttattt aaaataggta ataatacata aataaatagc gctacaattg caggcgtttt
     5341 accaacacca aaaatcggta tcataatagc aagtacagct agtgttggaa tagtttgtaa
     5401 gacacctgcc acagttaata caatattggc agttcgcttt gtttttgata ataaaatgcc
     5461 tataggtact gcaacaatga tggcaagtaa taatgccact atagaaatat agaaatgttc
     5521 cagtgttttc gacatcaact gtccaccatg ttcatgtagg aactcaatca tgatttatca
     5581 tctcctatgt cacgtacttt gacattagta gtgtgttgct catgcacttt tgaggacgca
     5641 gtgtcttccc ccacatgttc tgtttgcact gtatcctcac tatcgcccca aatcgtgtca
     5701 tatacaatat caacaacatt ggcacgcgta atcagtccta ctaaacgctg ttgatcatct
     5761 acgacaggta cattcctaac gtttcttttt aaaatcgtac gtacagaatc ttgtaattta
     5821 gaatcaattt gaacggtata aatatgttgt tgcatggtgt ctcgtaaact tttgtgtcca
     5881 cgtataccct gatttatatc ttcaatgtct aagaaaccta gtaaatggtt attactatct
     5941 actacaaaaa tagcatcaac acgtttttgt ctcataatat gaacggcgtc attcagtgtt
     6001 gcttctgctt gtatcgtgat tggtttaatc attacacctt ctacagtctt gtcattggga
     6061 cggtcttgaa tcagtctatt ttgtcctata aaatcacgta caaaatcatt tgcgggatgt
     6121 cttaaaatat tgtctggcgt atcaaattgc accaccttac cttctgacat aatacaaatt
     6181 ttgtctgcta atttaatcgc ttcatccata tcatgtgtta caaagataaa cgtcttgcct
     6241 aattttcgtt gtaacgtttt aactaaatct tgtaacgtat ctctcgtaat aggatccaat
     6301 gcaccaaaag gttcatccat taaaataata tcttgttcgg ccgcaagtgc tcttacaaca
     6361 ccgatacgtt gttgttgccc acctgatatt tctgctggat aacgctctaa aaatgactcc
     6421 ggtaaatcca caagtttaat taattccttt gcacgtttat ccttttcctc tttagtccat
     6481 ttcaacaatt tgggtaccaa cacaatattc tctttaatcg tcatatgagg cattaagcca
     6541 atttgttgaa taacatagcc aatatttcta cgcaattcga caggattcat actccgaaca
     6601 tctttaccat caatttcaat ttgtccttct gtcgcttcaa tcatacggtt tatcattctt
     6661 aaagcagtcg ttttgccact tccactggtt ccaataaatg cgataaattc cccagattga
     6721 atatctaaag agatgtcatc tactgccttt ttattaccag aataaatttt cgttaaatgc
     6781 ttaatactta acataatcat ttctccttcc tcataaaaaa tatgacgaat gatacgcata
     6841 aaataaaaag accattatcg caattgttaa acaaatagag tcataagtta aacaagtaca
     6901 catcatggtc ttgattatgt agaattttat atttttcatc tgtaatgtga atacttaaca
     6961 gtaaacggcg ctatgtatgt tgaatttcta ccgattactt tgtttgcaca ttcgtatatt
     7021 cgacatttaa aactaacata ctctatatga tgttattaaa cattattatt ttatctttgg
     7081 caactaaata gataactcat attttttaaa agcgaattta ttatcgctca attggttgtt
     7141 ttatagcaaa aaagtgtcga aaatgtgaaa tattattaca aaagtcgaat ttttgtaata
     7201 atgcacacgc atccattgtt acagacggac aaccgcccat ccgccactca caaagtcatc
     7261 cttatatttt aatatcttca tcattcattg tagacaccct tgccttttgc taacattttc
     7321 tactcccaag cctgtaacgg aattccaacg tcaaagtgtt gcccatgaca gacacaccta
     7381 aaaaaacaac caacagcatg agccattggt tgtttcgtgt catcttgtat gttgttgtat
     7441 aaccggtatg ctttactcat taattactta tcatgcatcc ctttaccttc tagtatttgg
     7501 ttaatatact tttcaacacg cttttgtctt gtttcactgc gtttagcttg tccaatatga
     7561 tatatatatt gatgttgtct gcctggcgtt aatttataaa atgaagactc taactgtggc
     7621 atttcttcga acttagcttc taactcttta ggaataacat attcctctgt tttcttcatt
     7681 tcaacttttt taccagcttt ttcagcttta attgcttcgg ctagataata tttaatttct
     7741 tcggtacgtg cttgaatctc tgttaaattt tcaaatcgta actgacgagc tgcttgcacc
     7801 ttttcagtct gttgaatgag tgtatgatat ttatcctcca aaatggcacc tttatgaaat
     7861 aatagtgcaa cataattttt gaagccatgg ataagtacta catttttatt attcaacgtg
     7921 taacaaggat gcatccattt atacgcttcc tctaattctg tttcattaaa gattaacgct
     7981 ctcaaatatt tatattcttc ttgccattga ctctctttat ttaaaaatgc ttcaacttgt
     8041 tcatttgatt gtttctcact catagtcatc acttccattt tgtttttcaa tacacattgt
     8101 attatatatc ctaattttat aaaaaactaa ttttacaaat gcttttgcgt tcttacaaaa
     8161 aatgcatttg actattatta taataagcgt ataattgtcg catattattt tttgtatttt
     8221 tggcaataac gaaggagtat ttatgaataa agacaagcaa ttgcacaacg acaaaatcaa
     8281 tctatcccaa ttagtcttat tagggttagg ctctttaata ggatctggtt ggctatttgg
     8341 tgcgtgggaa gcatcatcaa tagctggacc agcagcaatc atatcatggg ttcttggatt
     8401 cctagtcatt ggaaccattg cctataacta cattgaaatc ggcacaatgt ttcctcaatc
     8461 aggtggcatg agtaactatg cccagtatac acatggctca ttattaggct ttattgctgc
     8521 ttgggcgaat tgggtgtctt tggtgacaat aatacctatc gaagctgtgt cagctgttca
     8581 atatatgagt tcttggccgt ggcattgggc gaaaccaatg agatatttaa tggaaaatgg
     8641 ctctattagc acatacggat tgctagctgt atatctcatc attgttattt tttcattatt
     8701 aaactattgg tccgtaaaac ttttaacatc atttacgagt ttaatttctg tatttaaatt
     8761 aggcgtaccc atgttaacca tcatcatgtt gatgctatca ggattcgaca cttcaaatta
     8821 cggccattcg gcaagcacat ttatgcctta cggaagtgca ccgatttttg ctgcaacaac
     8881 agcatcaggg attatttttt cattcaattc attccagaca attattaata tgggttcaga
     8941 aattaaaaat cctgaaaaaa atatcgcaag aggcatcgct atctcactgt caatcagtgc
     9001 agtgttgtac atcattttac aaagtacgtt tatcacttct atgcctcaat caatgttaca
     9061 acatagtgga tggaatggca tcaacttcaa ttcaccattt gctgatttag ctatcttatt
     9121 aggaattaat tggctcgcaa ttttactata cattgaagct tttgtatcac cattcggtac
     9181 tggcgtgtca tttgtcgccg ttacaggtcg agttttacga gcaatggaga aaaatggaca
     9241 tatccctaaa tttcttggga agatgaatga aaaatatcat atcccacgtg tagcaatcat
     9301 ctttaatgcc atcattagta tgattatggt tacattattt agagattggg gtacgctagc
     9361 agcagttatt tctactgcaa ctttagtagc ctatttaact ggcccaacga cagtgattgc
     9421 attaagaaaa atgggaccaa caatgactcg tccatttaga gcaaaaattt taaaagtaat
     9481 ggcaccatta tcatttgtat tagcttcatt agctatatat tgggcaatgt ggccaacaac
     9541 ggctgaagtt attttaatca ttatacttgg attaccaatc tacttcttct atgaatatcg
     9601 tatgaattgg cgtaatacaa agaaacaaat tggtggtagc ttatggatta ttgtatattt
     9661 aatcgtgcta tcaatactgt catttatagg aagcaaagaa tttaaaggct taaatatgat
     9721 tcactatcca tttgacttta tcgttattat tattgtggca cttatcttct attacatcgg
     9781 tacaacgagt tcatttgaaa gcgtctattt ccgtcgcgca acacgaatca atacgaagat
     9841 gcgtgagtca ctaaataatg aatcaaagtc atctcattaa atattttaaa atacagtgcc
     9901 atatatgatt tcaacacaca cacattaacc aaccattgat ttcaacatct tggttggttt
     9961 tttattttga aaatcggtta taaataacta acataacaag atgatga
//
LOCUS       DANRDI010000099         9950 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.099, whole genome shotgun sequence.
ACCESSION   DANRDI010000099 DANRDI010000000
VERSION     DANRDI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.099"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            291..1328
                     /locus_tag="PZ377_001867"
     CDS             291..1328
                     /locus_tag="PZ377_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDM6358735.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            1344..1778
                     /locus_tag="PZ377_001868"
     CDS             1344..1778
                     /locus_tag="PZ377_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDM6358736.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(1841..2767)
                     /locus_tag="PZ377_001869"
     CDS             complement(1841..2767)
                     /locus_tag="PZ377_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDM6358737.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            3006..3260
                     /locus_tag="PZ377_001870"
     CDS             3006..3260
                     /locus_tag="PZ377_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDM6358738.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(3263..3652)
                     /locus_tag="PZ377_001871"
     CDS             complement(3263..3652)
                     /locus_tag="PZ377_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358739.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            3722..4264
                     /gene="rsmD"
                     /locus_tag="PZ377_001872"
     CDS             3722..4264
                     /gene="rsmD"
                     /locus_tag="PZ377_001872"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDM6358740.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            4266..4748
                     /gene="coaD"
                     /locus_tag="PZ377_001873"
     CDS             4266..4748
                     /gene="coaD"
                     /locus_tag="PZ377_001873"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDM6358741.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(4810..5949)
                     /locus_tag="PZ377_001874"
     CDS             complement(4810..5949)
                     /locus_tag="PZ377_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDM6358742.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            6076..6633
                     /locus_tag="PZ377_001875"
     CDS             6076..6633
                     /locus_tag="PZ377_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDM6358743.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLEVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            6713..6886
                     /gene="rpmF"
                     /locus_tag="PZ377_001876"
     CDS             6713..6886
                     /gene="rpmF"
                     /locus_tag="PZ377_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDM6358744.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(7040..8977)
                     /gene="isdB"
                     /locus_tag="PZ377_001877"
     CDS             complement(7040..8977)
                     /gene="isdB"
                     /locus_tag="PZ377_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HDM6358745.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(9180..>9950)
                     /gene="isdA"
                     /locus_tag="PZ377_001878"
     CDS             complement(9180..>9950)
                     /gene="isdA"
                     /locus_tag="PZ377_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDM6358746.1"
                     /translation="IKQNNKYYFQTVLNNASFWKEYKFYNANNQELATTVVNDNKKAD
                     TRTINVAVEPGYKSLTTKVHIVVPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQ
                     PKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHT
                     VKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKE
                     LPKTGLTSVDNFISTVAFATLALLGSLSLLLFKRKESK"
BASE COUNT         3152 a         1374 c         1763 g         3661 t
ORIGIN      
        1 tttgttttat caggtttttt gatgaagaat agtcggacag ttgatggtat tcatttgata
       61 ccttaactgt ccattttttt atacatattt taatcttaat aatgaaattc aacctgaaaa
      121 cataaagata ggttatgata tagagtgtag ggaaaatctt tatgacgtta aaattatgcc
      181 tgaagatgat tttgttttat cgaaacgaca cagttatgtc agtgttgaaa atttgctaat
      241 taatgtgttg ttttttacaa tagtattaat actttttagg tggttgattg atgaaaaagt
      301 taattataag agtcgtcggt gtattatttt tagttggatt tttaatatat cttttttatt
      361 caccgagatt aaaatttgat gtattagaga acccgaataa aggtaataaa gtaaatagat
      421 ctgaacaggt gaataaatca aataaccatg ctgaaaatcc aaagcctaaa gaaggtgttg
      481 gtacatgggt aggtaaagat attaaagtgc ttacttctaa atttggacaa gcagatcgtg
      541 tctacccttt tagagatggt tacaaaaatt atgtgtttaa agacaaaaac agttattaca
      601 ttgtttcaac taaacgtgaa gaaatcgttt cagtgtatgc tacaggtgag aaagtcaatg
      661 ttagtccgtt aaaaatagga caacattctg cagaaatttt taatcataca agtattaatc
      721 cagaaccgtc ctttaaagtt gatggtaaaa aatatgaatt tgaactttca gatgaagatt
      781 taaaaacaca aacactgatt aaatatggcg acatatatgc acaagtgtat tctgatcaac
      841 aatcgaaaaa ggtgctaagt gtacgatttt taacaaaaga aatgttagca gatattgaac
      901 cttatcaatt aaattctaat tctacgtcag aagagcataa taagcgtcca gttgagcaaa
      961 atccaaatca attaatttct ctttatgaag taacgaatga aatgagaaaa ttaaaaggat
     1021 taaaaccatt gaaaatcaat agcgatttag cacatattgc atctaataac ttatatgaag
     1081 cgacctctaa tggttctgat agtgttgaat ttacagagga cgcattaaga gggcaattag
     1141 ataaaaatca cgttacttat aaaacaactg ctcaaaatgt tggttatgcg tttaatgatg
     1201 taccaacatt aatccatagt tggatgaatt cagatataca tcgatctcgt ctattaaatt
     1261 caaaatacga tgagatgggt ggagatgtaa tgagagatta ttattcacta attttcttag
     1321 aaaaataaca atgaggggta tttatgatta atgaagcttc actagcgata ttagatgata
     1381 ttgatgaact agctgatatg atagttgcat cagatattta tgcatcattt gagcaagcta
     1441 aacaggcgct cgaaaacaat gacgaagcac atttacttta tcaatcattt ttaaaatcaa
     1501 aagagaaata tgatgaagta atgcgattcg ggaaatatca tcctgattat aagaaagtta
     1561 tgttagagac aagacaacgc aaaagagcat atgagatgct tgacgttgtg atgcattaca
     1621 aagctaggga aatggcactt caacatttaa tagatgaagt tgttacgaaa attgcgtacg
     1681 ctgtttcaga acatgtcaaa atagaaacag gtaatccatt ctttcaaaca tcacatagtg
     1741 gttgtgcgac gggcggatcc tgtaattgtt cattataaaa aacatcgagt cagaaaaagg
     1801 tggttattga aaccactaac tagcatctga ctcgatgttt ttatttattc gggattgttt
     1861 gtttgaattg ttgtgctaaa tctggtcgat ctgtcacaat cgtgtgtgca cctttttggt
     1921 ataaatcatt catcagattt atactattta cgccataata gcctggaatg atattcatat
     1981 catttaacca tttgataaaa cgagatgaag tcaaatcaat gcctttaaaa tgagtaggca
     2041 tttggaacgt ttgtgctaat ggttggtagt acctaccacc taataaatga tattttaaaa
     2101 atgcttctgt aacttcctgt tggctagcac caattgcgac ggatccttgt gcaattttat
     2161 taaaacgaac gatttgttct ttataaaaac ttgtcacaag aacgcggtca aatgcttgat
     2221 tttctgcaat tgtatcaaac ataatttgtg gtgcgattga gccttcatag gattcaggag
     2281 catcttttaa gtctacgttt atatacatat caggatattg cttcagcaac tcatcgaagg
     2341 ttagtatagc tgtgtgtgca tgaccacgat atggtgttag tccattgata tctttgaagt
     2401 gataagctgc gtctaatttc tttaattctg ctaatgtatg ggcactaact tttccagagc
     2461 cgttcgtcgt tctatcaaca gttgcgtcat gaaaaacgat aagctgttga tcttttgtga
     2521 gtctcacatc tgtttcaaag ccatcaacgc ctaattgttt agcatagtca aatgcaagtt
     2581 gcgtttgctc tggtcttaaa gccataccac cgcgatgcgc aaatatatat ggtgcattgc
     2641 ctttgaaaaa agcagggatg gtttgctttt tagtaatcac tttattttta ttgatcatta
     2701 atagactact taaaaatcca gcaccgacta gtaccgcatt taaaatgttt ctgtttactt
     2761 ttttcataaa aaattcctcc tcgtttactt ttatagctta acaaattcat agttctgact
     2821 acagcgaaat gtgtggttaa tcatatagtg atgctcttga gaattgaatt aatataatga
     2881 tgtttaatat aatctaaata catttatcga gcaattacat aagacataat taaggtagct
     2941 taatatttta aaattaataa agacatgata tgattaatgc tgaaaataaa tggagtgagg
     3001 tttaaatgaa tttaatccca agaactagta ttgtagttta tttaaaacat atgaaacatg
     3061 aacgacaaat ccgaaaatat ggacatatcg ttcattcaaa tagagatcgt aaatttgtaa
     3121 ttatgtatgt gaatgagcaa gatgttgatc aaattgtaca taaactaatg caacttaaat
     3181 acgttagaca cattgatggc tcaccatata aatacttaaa gaaaacttac gaaaaagaga
     3241 aacacgaaat atataattaa tattattgaa gttctggtat ccatttttgg agccttaaca
     3301 cactaattaa ataattataa tataacgata aatcatgaaa tgcttgaggg ggctgtacat
     3361 cgatacctaa aattggtatg tcgaattttt cagcacaatc atttatcatt tgaaactttt
     3421 tatttgaatc tggatatgga tatttaatag cgtttatcat gatataaaat gcttggaaat
     3481 gattgccagt tgtagggttc ataatgattg ctacagatga ctttttattt tcagcttgtg
     3541 gtgaatgaaa atagataatt ttatcaatac gttgatggtt atattcaatc atcgtcatga
     3601 attcgaacaa atctgttaga ccttcaccaa gtgtaataaa tgtttgtttc attggtttat
     3661 cattctcctt tttctcaata aattatatta aataactaaa tgaattggaa gtgtgaacaa
     3721 catgcgcgtc attgcaggta aacataaaag taaagcttta gaaagtatgg aaggccgtaa
     3781 tacgagacca actatggata aagttaaaga aggtatcttt aatagtttat atgatgtgtc
     3841 aggtataggt ttagatttat ttgcaggaag cggggcgctt ggaatagaag cactctctcg
     3901 aggtatggat aaggtaatct ttgttgatca aaattttaaa gctgtaaaag ttattaaatc
     3961 aaatcttgcg aatttggatt tagaggcaca atctgaagtt tataaaaata atgcagatag
     4021 agctttaaaa gcattgtcaa aacgtgatat tcaatttgat gtcattttct tagatccacc
     4081 ttataataaa ggtctcattg ataaagcttt aaaactaatt tcagagttta atttattgaa
     4141 agaaaatggt atcatcgttt gtgaatttag caatcatgaa gaaatagatt atcaaccgtt
     4201 taatatgatt aaacgttacc attatgggtt gacagacaca ttgttattag aaaagggaga
     4261 atagcatgga acatacaata gcggtcattc cgggtagttt tgaccccatt acttatggtc
     4321 atttagacat tattgagaga agtacagata gatttgatga aattcatgtc tgtgttctta
     4381 aaaatagtaa aaaagaaggt acgtttagtt tagaagagcg tatggattta attgaacaat
     4441 ctgttaaaca tttacctaat gtcaaggttc atcaatttag tggtttacta gtcgattatt
     4501 gtgaacaagt aggagctaaa acaatcatac gtggtttaag agcagtcagt gattttgaat
     4561 atgaattacg cttaacttca atgaataaaa agttgaacaa tgaaattgaa acgttatata
     4621 tgatgtctag tactaattat tcatttataa gttcaagtat tgttaaagaa gttgcagctt
     4681 atcgagcaga tatttctgaa ttcgttccac cttatgttga aaaggcattg aagaagaaat
     4741 ttaagtaata aaaataacag tattttaggt ttatcatggt ttacaatcct aaaatactgt
     4801 tttcatttgt taacgatatt gctgtatgac aggcgtgttg aaatctgttt gttgttgccc
     4861 gcttattgca ttgtatatgt gtgttgcttt gatttcattt gtgaagtaat gtgcattgct
     4921 tttgttaata ttggttatat attgtctttc tgggaacgct gtttttaaat gctttaaata
     4981 ttgtctgcca cggtcgttca tcgctaatac tttaactgcg tgaatgttac tcgtaacatc
     5041 tgtaggttta atgtttaata atacattcat taacagtctt tggatatgcg tatatgtata
     5101 acgctttgtt tttagtaatt ttacaaaatg atgaaaatca gttgcttcat aaatgttaga
     5161 tttcaaacga ttttcaaaac cttcagtaac agtataaata ttttttaatg aatctgtagt
     5221 catagctatg atttgatatt tcaaatatgg aaatatttga tttaatgtta tatgaggtgt
     5281 tacgtacaag tgttgaatat ctttaggtac cacatgatgc caatgatcat cttgactaat
     5341 gattgatgtt ctaatagatg taccacttgc aaactgatgg tgttgaatta atgaatcatg
     5401 atgttgagca ttttctcgtt tgatagaaat tgcattgatg tttttagcat ttttagcaat
     5461 tgctttcagg taactaatac caagtatgtt gttaggactt gctagtgctt catgatgctc
     5521 taataattcg ctaatgatac gagggtagct tttaccttct tttacttttt gtgaaaagga
     5581 ttcagattgt tcaatttcat taatgctgtg tgctaattgc tttaatgttt tgatatcatt
     5641 attttcacta ccaaatgcaa ttgtatcgac actcatataa tctgcgactt taactgctag
     5701 ttcggcaaaa tgatcgccag atgataaact ggcagttgct ggtagttcga taactaaatc
     5761 agctgttgat aatgccattt ttgcacgagt aaacttatta tagattgctg gttcgccacg
     5821 catgacaaag ttaccactca ttattgcaat agtaacgtca gcatttgtaa gttttttaga
     5881 ttgattaata tgatattgat gcccattatg aaagggatta tattctgtga ttaagccaac
     5941 gcttttcatt tgcaattcgt cctttcggta ttcattatta atattgtaac agaacatgat
     6001 atgttaagaa aaaatcttga caacttgttc ttagaaagtt aaaataaatt ttgtgcttgt
     6061 tagaggtgaa gccatatgaa atggtcaatt acgcaattaa ggaaatatca aggtaagcca
     6121 tttgaatttg atcaaacggt gagttttgac aatttaaaag aatcattaga tttaattgat
     6181 ttatctccaa ttacaatcca aggtcagtta accattaagt caacagaagt cgttgcggat
     6241 attcacatta ctggaacgta tacaatgcct tgtgcacgta ctcttgtacc agtaaaagtc
     6301 ccactagaag taactactac agaagtattt gatttagaag ggtacaatca gtataacgat
     6361 gatcaagatg atgtagatga acactatcac attattaaag atggtatggt taatcttcag
     6421 gatattgtcg aggatatagt tattattgag aaaccaatga gagcttattc agagcaaagt
     6481 gaccaaatgt tgacagtagg taatggttgg gaagtaatcg atgaggatca attagatgag
     6541 cttgctaaac agcaagaaca agatgattca gaatcacgac aagttgatcc aaggcttcaa
     6601 aaattacaac aattatatga taaagagcaa taagtagttt aattaatgat ataatgttgt
     6661 taattaaact aacatttagt atttttcgtt tataatctaa ggaggatata tcatggcagt
     6721 accaaaaaga agaacttcta aaactagaaa aaacaaacgt cgtacgcatt tcaaaatttc
     6781 agtaccaggt atgactgaat gcccaaactg tggcgaatac aaattatcac accgtgtatg
     6841 taaaaactgt ggttcttaca atggcgaaga agtagcagct aaataatttt agttactcaa
     6901 tataaaaagt cccgcttaaa atgattgttt taagtgggac tttttatatt gcgaaaaata
     6961 attggcgaac gaggtaactg gatacctcat ccgccaatta aaatttgtta atttaataat
     7021 taaatataaa gacgatttat tagtttttac gttttctagg taatacgaat gcaacgatgc
     7081 tacttaaagc taataatgcc attaatggta atgtcatatc tttatttgat tcttcaccag
     7141 tttgtggtaa tgattttgct ttattttctt gtgtattttt attgttttgg ctttgagtgt
     7201 gtccatcatt tgtgttttta atgtttgctt tttgtaatgg agcactatct tttgcttcgc
     7261 tagaacctgc tgaagtttga acaacatctt ttgttgtttt tgatgaagca gttgttggtt
     7321 ttgcaacatt ttgagtcgta gatactacct tagttggagt tgtactactt gattctactt
     7381 cacctttagt tggttttgta gcaggcgttt tgtctttacc tgactcacta gatgcgtcat
     7441 tttctttttc aacacttggt aattgtttat tgtcatcttt ttggctgtct tgtttttgtg
     7501 attctttttc aacaggtgat ggtgttggtt tgctaggcgt agctggagta gcttccttct
     7561 tagctgagtt atcttgttgt tcttttttgt tagatttatc ggtattggct tttgtaaatg
     7621 cttctttatc aacgattctg acatggtatt gtccatcata atcaatcgtt tttacgtgaa
     7681 ctttaacgat agcatcatat agagttttac cttcaacata tgggaaaata attgttctag
     7741 tattattttt agcatctttg cttatagttc taacacgttg accttcaacc atgaaatctt
     7801 tccagtaatc gtcattagta gtttccatga ccatatattt tttgccgtta agcatacctg
     7861 ttttaatagg gtgtttaaca aaagtatcca tcatagattc gttattctca acactttcat
     7921 aaacaacata ttttgtatct tgtaaatcag tcattttttc atttgttggt tgtacatttt
     7981 ggaattcagt aatagctgat ttcacttgct catctaaagc tttctttgta tcctctaatt
     8041 tcttcttgta ctcagccttt aatttttcag gaagtttatc ttgaatttta tttaattcat
     8101 aaacttgtct ttctagtgtt ttcgcttttt tatatggcgc taataatttt tcagctttat
     8161 aatcttcttc agttttgaat ttatctgcac tgttataaat tggttgtgcg aattccatta
     8221 atgtgtaatc gtatttttct tctttgttat tgaagtgtgt tgaactaaca attttaacag
     8281 cttttgttcc gtttgataca gagaagcgaa tgtaagcata atctttaaca gtatcgtatg
     8341 atactaattt aattggcaac tttttgtcac cttcataaac ttcaaatttt ctccaaaatt
     8401 gacctgattg taatcctaat tcaatttctg gttttgaatc agtgaaaata actctagcag
     8461 gtttaacaga acttgcataa tgataaaact gttgagttcc atctttcttt ttcatttcaa
     8521 aatcaattgg acgagagttt ggtgcgctat gatctttgtc ttttattgca gggtttttaa
     8581 tcgcttctct aagttcctga ttcaaaatag gatatgtatt gttagtggct tttgctgctg
     8641 gtttaacttc ttttgtttcc ttaggggctt taacttcttt aacttcttta gcttcttttg
     8701 tttcagaagt aggggcctca acttctttat tagatactga gacagcatta gctactggtt
     8761 tagtttctgg agctttttca gatgttgttg ttggacttgc aactgcttca gtttttggtt
     8821 gtgcttctgt atttgtacca cctgtttctt cagctgctgc ttgtgcttcg ccatttgaca
     8881 ttaataataa aagtgtacta attgctacag atgcaacgcc tagtgatgac tttctaattg
     8941 aataaaatga tttaaattct ttttgctgtt tgttcatgtt gtagaaacaa ctcctaattg
     9001 tatattatca actgataatc attatcaatt gattacttgt attgtacatt gaaatgttag
     9061 ttaattgcaa cgttatttta taaaaaaata acctatgtta tagattatga aatatctatg
     9121 acataggtta aataaaattt aaaataaaaa atatattaag ttatgagtgt gacgatgatt
     9181 tatttagatt cttttctttt gaaaagtaat aaagataatg aacctaaaag ggcaagtgtt
     9241 gcgaaggcaa ctgtgctaat aaagttatca actgaagtta aaccagtttt tggtaattct
     9301 ttagctttag atgcttgttt aggcgtttcg ttatgttttg taactttgtt agtttgttgt
     9361 gatttattat cacttacagc ttgattgttg ctttcagatt tcgctgttgc aacatcttta
     9421 acaggtgttt gaactttatt ttgttcttga gcagtttgtg ctgttttaac tgtatgagca
     9481 gtttgtgttt tagtttgatc ttttgttgtg tcagtactta caactttagt agagtgattg
     9541 tcttcaactt tgcttgttgt agttacagta ggtttaactt tttcaacttt aggttgaact
     9601 ggtttagttt gctcagtagg tgttttaggt tgagctggtt ttggttgaac cggtttaaca
     9661 ttgtttggtt ttgctgcgtc agctaatgta ggaattgctt tttcaaattc caaatgcgta
     9721 gtatatctat gattgtaatt aatttgtggc acgacaatat gtactttagt agttaagctc
     9781 ttatatccag gttcaactgc aacattgatt gttctagtat ccgctttttt attatcgtta
     9841 acaacagttg ttgctaattc ttgattgttt gcattgtaaa atttgtattc tttccagaat
     9901 gatgcattgt ttaacacggt ttggaaataa tatttattat tttgtttaat
//
LOCUS       DANRDI010000100         9661 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.100, whole genome shotgun sequence.
ACCESSION   DANRDI010000100 DANRDI010000000
VERSION     DANRDI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.100"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..295
                     /locus_tag="PZ377_001879"
     CDS             <1..295
                     /locus_tag="PZ377_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDM6358747.1"
                     /translation="KVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGLTILNGH
                     GYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLADE"
     gene            577..1374
                     /locus_tag="PZ377_001880"
     CDS             577..1374
                     /locus_tag="PZ377_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358748.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            1410..2414
                     /locus_tag="PZ377_001881"
     CDS             1410..2414
                     /locus_tag="PZ377_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDM6358749.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            2411..3427
                     /locus_tag="PZ377_001882"
     CDS             2411..3427
                     /locus_tag="PZ377_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDM6358750.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            3661..4629
                     /gene="dhaK"
                     /locus_tag="PZ377_001883"
     CDS             3661..4629
                     /gene="dhaK"
                     /locus_tag="PZ377_001883"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDM6358751.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            4671..5255
                     /gene="dhaL"
                     /locus_tag="PZ377_001884"
     CDS             4671..5255
                     /gene="dhaL"
                     /locus_tag="PZ377_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDM6358752.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            5248..5610
                     /locus_tag="PZ377_001885"
     CDS             5248..5610
                     /locus_tag="PZ377_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDM6358753.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            5726..6223
                     /locus_tag="PZ377_001886"
     CDS             5726..6223
                     /locus_tag="PZ377_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358754.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            6679..7746
                     /locus_tag="PZ377_001887"
     CDS             6679..7746
                     /locus_tag="PZ377_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358755.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            7897..8940
                     /locus_tag="PZ377_001888"
     CDS             7897..8940
                     /locus_tag="PZ377_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6358756.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            9269..>9661
                     /locus_tag="PZ377_001889"
     CDS             9269..>9661
                     /locus_tag="PZ377_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358757.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDID"
BASE COUNT         3347 a         1324 c         1826 g         3164 t
ORIGIN      
        1 aaaagtgatg gaatatgtca tagaaggttt aaacactaaa aaagctatga cgattatttc
       61 aactaatccc gacaaacttg ccaaagcaat agacgagcaa attggaagag gtttaaccat
      121 tttaaacgga catggctatt atacgcgtga agaaaaagat gtcttatacg ttgttatttc
      181 taaaacacaa gtttcaaaag caaagcgatt aattaaacaa atcgataaag atgcattcct
      241 cgtaattcat gatgtaagag atgtctatgg taatggcttt cttgcagatg aataaataaa
      301 tggtatgagc acacatactt aaatagaagt ccacggacaa gtttttgaac tatgaagact
      361 tatctgtggg cgttttttat tttataaaag taatatacaa gacatgacaa atcgagctat
      421 ccaatttaaa aagtaatgtt agtcaataag attgaaaaat gttataatga tgttcatgat
      481 aatcattatc aattgggatg tctttgaaaa ttgataattt aaaaatagaa attatttttt
      541 ataaacagaa agaattttat tgaaagtagg gaaattatga atcgtttgca tggacaacaa
      601 gttaaaattg gttacgggga taacacgatt ataaataaat tagatgttga aataccagat
      661 ggcaaagtga cgtcaatcat tggtcctaac ggctgcggga aatctacttt gctaaaggca
      721 ttgtcacgtt tattggcagt taaagaaggc gaagtatttt tagatggtga aaatattcat
      781 acacaatcta cgaaagagat tgcaaaaaaa atagccattt tacctcaatc acctgaagta
      841 gcagatggct taactgttgg ggaattagtt tcatatggtc gttttccaca tcaaaaagga
      901 tttggtagat taactgctga ggataagaaa gaaattgatt gggcaatgga agttacagga
      961 actgatacat tccgacaccg ttcaatcaat gatttaagtg gtggtcaaag acaacgtgtt
     1021 tggattgcaa tggcattagc acaaagaact gatattatct ttttagacga accaacaaca
     1081 tatttagata tctgtcatca attagaaata ctagaattag ttcagaagct aaatcaggaa
     1141 caaggttgta caattgtcat ggttcttcat gatatcaacc aagcgattcg tttctcagat
     1201 catcttattg cgatgaaaga aggggatatc atcgctacag gttcaacaga agacgtatta
     1261 acacaggaaa tattagaaaa agtttttaat attgatgttg ttttaagtaa agatcctaaa
     1321 actggaaaac ctttactggt aacttatgac ttatgtcgca gagcttattc ttaattaagt
     1381 aagttaatat gataaaaagg acaattaaca tgacaaatag agagaaccca acgccattga
     1441 agtttttatc ctatattata ggtttaagta tgatactact aatcacacta tttatttcta
     1501 cattaatagg tgacgccaaa attcaagcct ctacaattat agaggctatt tttaattata
     1561 atcctagcaa tcaacagcaa aacatcatca atgagattag gattcccaga aatatagcag
     1621 cagtaattgt aggtatggcg cttgcagttt ctggtgcgat tatacaaggt gttactcgta
     1681 atggtcttgc tgatccggcg ctcataggtt taaattcagg tgcttcattt gctttagcat
     1741 taacatatgc agttttacca aacacttcat ttttaatatt gatgtttgct ggatttttag
     1801 gtgctattct aggaggtgct attgtattaa tgataggccg atctagacgt gatggattta
     1861 atccgatgcg tattatttta gcgggtgcag cagtaagtgc tatgttaaca gcgctaagtc
     1921 aaggtattgc attagctttt agactaaatc aaacagtaac attttggact gctggaggcg
     1981 tttcaggcac aacatggtca caccttaagt gggcaattcc attaattggt attgcgttat
     2041 tcattatatt aacaattagt aaacaactta ccattttaaa tcttggtgaa tcattagcta
     2101 aaggtttagg tcaaaatgta acaatgatca gaggcatatg tttaattatt gctatgattc
     2161 tagcaggtat tgcagttgct atcgctggac aagttgcatt tgtaggtttg atggtacctc
     2221 atatagcaag atttttaatt ggaactgatt atgctaaaat tctaccatta acagccttgt
     2281 taggtgggat actcgtgctt gttgccgatg tgatagcacg atatttagga gaagcgcctg
     2341 ttggtgcaat catttcattt atcggtgttc cttacttttt atatttagtt aaaaaaggag
     2401 gacgctcaat atgattagtt caaataataa acgcagacaa ttgatagcac tggctgtttt
     2461 tagcattcta ctatttctag gttgtacttg gagtattacc tcaggtgaat acaacatacc
     2521 tgttgaaaga tttttcaaaa ctttaattgg acaaggtgat gccattgata agttaatctt
     2581 attagatttc aggttacctc ggatgatgat tactattttg gctggcgcag cgcttagtat
     2641 tagtggtgca atagtgcaaa gtgtcacaaa aaatccaata gctgaaccag gtatattagg
     2701 tattaacgca ggtggcggat ttgcaatcgc attatttatt gcaattggta aaattaatgc
     2761 tgacaacttt gtttatgtac tgccgttaat aagtatacta ggtggtatca ccactgcatt
     2821 gattattttt attttcagtt ttaataaaaa tgaaggtgtt acacctgcga gtatggtatt
     2881 aataggtgta ggtttacaaa cagcattata tggtggctca attacaatta tgtcaaaatt
     2941 tgatgataag caatctgatt tcatcgctgc ttggtttgca ggtaatattt ggggtgacga
     3001 atggccattt gtcattgcat ttttaccgtg ggtgttgatt attattcctt acttactatt
     3061 taaatcgaat acactaaata ttattcatac gggtgataat attgcacgag gtctaggtgt
     3121 aaggttaagc agagaacgtt taatattatt ctttatcgca gtgatgttat catctgctgc
     3181 tgtagcagta gcaggttcaa tttcgtttat cggattaatg ggtccgcata ttgccaaacg
     3241 tatcgttgga ccacgtcacc agttgttttt accaattgcc attttagtag gggcatgttt
     3301 acttgttata gctgatacaa ttggcaaaat tgtattacaa ccaggtgggg ttccagcagg
     3361 tattgtcgta gcaattattg gtgcaccgta tttcttatat ttaatgtaca aaacgaaaaa
     3421 tgtatagtgt caatggacac aacttattgc tatgaaaggc actttattat aaggcttttc
     3481 atagcatttt ttatttaatg agccactcaa gactatttat tttttcaata atgaaccatt
     3541 aagttatcaa gaggatctta tcaaaaatat atttgataac ggtatcaggt taattcttta
     3601 tgatagcgca ttcatttatt ctgttttata ctatgactga taataccaag gaggtacaac
     3661 atgatgaaaa agttaatcaa taaaaaagaa acatttttaa ctgatatgct tgaaggattg
     3721 ttaattgcgc acccagagtt agatctgatt gctaatacag ttattgtaaa aaaagctaag
     3781 aaagaacatg gtgtagcaat agtctctgga ggtggaagcg gacatgaacc tgcgcatgcc
     3841 ggttttgttg cagaaggtat gctagatgca gcggtttgtg gcgaagtatt tacatcacct
     3901 acacctgata aaatattaga agctattaaa gcagtagata ctggtgatgg tgtattacta
     3961 gttgtaaaaa actatgcagg tgacgtgatg aatttcgaaa tggcacaaga gcttgcagaa
     4021 atggaaggta taaatgttca aactgttatt gttcgtgacg acattgctgt gacaaacgaa
     4081 gtacaacgtc gtggtgttgc aggaacagtg tttgttcata agcttgccgg ttatcttgct
     4141 gaaaaaggtt attcattaac agagataaaa tcgcgtgtag aagcgttgtt acctgaaatt
     4201 aaaagtattg gtatggcaat tgagccaccg cttgttccaa ctactggaaa atatggcttt
     4261 gatattgaag acgacaaaat ggaaatcggt attggtatac atggtgaaaa aggtattcat
     4321 agggaagaag taaaggatat tgatcatatt gttggaacat tgttagacga attgtataaa
     4381 gaagttactg ccaatgatgt catattaatg gtaaatggta tgggtggtac gccgttatct
     4441 gaattaaata tcgtaactaa atatattcaa caaaatttag ctgcaagaac ggttaatgtt
     4501 gctaaatggt ttgttggtga ttatatgaca tctttagaca tgcaaggttt ttctataact
     4561 atcgtgccta ataaaccaga atatttggaa gcatttttag caccaacaac aagtcaatac
     4621 tttaaataag aagtgaatat gaataaatat acatttatga ggtggcacaa atgaaagtga
     4681 atgatatgaa agcacgttta ttaaatttag aagaaacgtt taaaaaacat gaatctgaat
     4741 taactgaatt agatcgagca attggtgatg gtgaccacgg ggttaacatg gttcgtgggt
     4801 ttagtagtct taaagacaaa cttgatgata gctcaatgca atcattgttc aaatcaactg
     4861 gtatggcatt gatgtcaaat gttgggggtg catcaggacc actgtatggc tttagctttg
     4921 ttaaaatgtc tgcagtcacc aaagatgata tggataatca agatttcatt acactaattc
     4981 aggcatttgc cgaagcggtt gaatcacgtg gtaaagttac tttaaatgaa aagacaatgt
     5041 atgatgtagt agcgcgagca gcagagaagc ttaaaaatgg tgaaacttta acattcaatg
     5101 atttacagca attagcagat aatacaaaag atatggtagc aacgaaaggt agagctgcat
     5161 attttggaga agaatcaaaa ggttatattg atccaggtgc tcaaagtatg gtttatattt
     5221 taaacgcttt gattggagat gaagataatg cctaaaatta tacttgttag ccacagtaaa
     5281 gaaattgcaa gtggtacaaa atctttgtta aagcaaatgg caggtgacgt tgatattata
     5341 ccaatcgggg gattaccaga tggttcaatt ggaacttcat ttgatatcat ccaagaagtt
     5401 ttgactaaat tagaggatga tgcattgtgt ttttacgata ttggatcttc agaaatgaat
     5461 gtagatatgg caattgaaat gtatgatggt aatcatcgtg tgttaaaagt tgatgcacca
     5521 attgttgaag gcagttttat cgcagcagta aagctatcaa tcggcggttc aattgatgat
     5581 gcattagcag aaatcaaaca atcattttag ttaaaattta ctaataatga aaaatgtaaa
     5641 cctttttcaa atgaaacttt ataaaaaata tgatagtata tatgtaaatg tttaataaaa
     5701 tctggagaaa taggaggaca ttgccatgca acaccttata aaaaaacatg tattgaatgg
     5761 cgagtttgat ttagtacgac aattgatgtc cgaaacagat tttatggaat ttgaagaagc
     5821 atatatttca agtgcgcatg aagtagaaag tatgatgttt tatacatgta ttttagatat
     5881 gattaagtac gaagaatcat ctgaaatgca tgacttagca tttttattgc ttgtgtatcc
     5941 actaagtgaa tatgaaggtg ctttggattc tgcttattat catgcagacg cttccataaa
     6001 acttactgac ggcaaagaag ttaaaagttt gttacaaatg ttattattgc atgcgatacc
     6061 aacacctgtt atttcagata agaaggcttt tgatatcgcc aagcaaattt taaaattaga
     6121 tcctaataat aatgttgctc gtaacgtctt aaaagacact gccaaacgta tggacaacgt
     6181 tgttgttgat ataaatgaat tacaccaacg taatgcacgt taattacatt tcaattatat
     6241 tagcttaata atagttttaa catttggttg ggttgggcat atgttccagc cttttttaat
     6301 acttaaaaac taacgaagta tacttgtgtg cacaaatggt ttttatacaa cattttataa
     6361 atttatacat tttaataaag aacatacgat agatggttta aaccttgtta actgagaaat
     6421 tttgatatgt attcttcgaa atttaactaa atatacgaaa ttcaagaagc acaataatta
     6481 atcatttttc ctatacaaaa gttcgtatga ctgcattata aaagcataaa tttataattt
     6541 ttttaaatgt cattgaacgt gataatgtga atggattgag caattttgaa aaagtgaaaa
     6601 ataacctatg cggcttgcaa ttaattttca gtacgttata atgcacactg tgcaaaatta
     6661 aggaggtcta ttattcacat gatgatgaat aaagaagcaa caaaaattgg atttgcctac
     6721 gtcggcattg tagtgggcgc aggattttca actggacaag aagttatgca atttttcact
     6781 aaatatggct tgtgggctta tttaggtgtt attatatctg gttttatttt agcttttatt
     6841 gggcgccaag tagcaaaaat tggtactgcc tttgaagcga caaatcatga atcaacatta
     6901 caatacgtat tcggtgaaaa gtttagtaaa gtctttgatt atattttaat cttcttctta
     6961 tttggtatag ctgtaaccat gatagctggt gcaggcgcaa catttgaaga aagttataac
     7021 atacctacat ggctaggtgc tttaattatg acattagcga tttatattac gttgctatta
     7081 gactttaata aaatagtacg tgcactaggt atcgttacac catttttaat tgttttagtt
     7141 gtattaatcg ctggcgttta tttatttaaa ggtcatgttt cattagcaga agttaaccaa
     7201 gtagtgcctg aagcaagtat ttggaaggga atctggtttg gtacaatata tggtggatta
     7261 gctttttctg taggttttag taccatcgta gcaatcggtg gggatactga aaagcgtaca
     7321 gtgtcaggtg caggcgcgat gtatggtggt attatctata ctgtattact agcattgatc
     7381 aactttgcat tgcaaagtga atatccaact attaaaaatg cctcaattcc tacattgacg
     7441 ttagcaaata atatccatcc tttaatagca acagtgttat ctgttattat gctggcggtt
     7501 atgtataata ctattctagg actaatgtat tcatttgcag cacgttttac agaaccatac
     7561 agtaaaaatt atcatatctt tattattata atgatggtag caggttattt attaagtttc
     7621 gtaggatttg ctgaattaat taataagtta tatacaatta tgggatatgt aggcttattt
     7681 attgtagtag ctgtaattat taaatatttc aaacgtaaaa atgcggataa aaaacatatt
     7741 gcttaatatc atatgaggga tatccgaaac tttacaattg aatcactttg ttttaacctt
     7801 aaaagcaatt cgtctctact cttatcgggc gaattgcttt ttatatttat tcagtctatt
     7861 aatatgagcg tctaacaaat agagaggtac gatgtaatga ataaagataa taaatggacg
     7921 atgataactg cgctttttat aactgtaatc agtgtattgt tagcatttca tctgaaacaa
     7981 cattatgacc aaattacaaa tgagaaccat gctaataaag acaaaattaa tattaaaaat
     8041 aaaaatgtgc gcatttatca aaaccttaca tacaatagag ttttccctaa cagtaaatta
     8101 gatattatta cacctgttga tatgtcttct aatgccaaac tgccagttat tttttggatg
     8161 cacggtggtg gttatattgc gggtgataag cagtataaaa acccattatt agcgaaaatt
     8221 gctgaacaag ggtacattgt tgtgaatgta aattatgcat tggcgccaca atataaatat
     8281 cccacaccat taattcaaat gaatcaagca actcaattca ttaaagaaaa taaaatgaat
     8341 ttacctattg attttaatca agtaattatt ggcggtgatt ctgcaggtgc tcaattagct
     8401 agccaattta cggcaataca gacgaatgat cgcttaagag aagccatgaa atttgatcag
     8461 tcattcaaac catcgcaaat taaaggtgct atactatttg gtggttttta taatatgcaa
     8521 acagttagag aaactgagtt tccaagaata cagttattta tgaaaagtta tactggcgaa
     8581 gaagattggg aaaagagttt taaaaacatt tcacaaatgt cgacagtaaa acaatcgaca
     8641 aaaaattatc caccaacatt tttatctgtt ggagatagcg atccattcga aagtcaaaat
     8701 atagaattca gtaagaaatt acaagaattg aatgtaccag tagatacttt gttttatgat
     8761 ggtacgcatc atttacatca tcagtatcaa tttcacctta ataaacctga atcgatagat
     8821 aatatcaaaa aagtgttact tttcttaagt cgtaatacat cctctagtgg tattcaaact
     8881 gaagagaaac cacaaataga aaatccgagt aatgaattac cgttaaatcc tttaaactaa
     8941 tgataaacag tagtaattta ttgcttaagc aacatttaag attttcaaat taaaaacgaa
     9001 gaatttaaaa catgtggtgc taatgtgtaa gagtctgaga tataataaat tgttagcggt
     9061 tctttatcat ttctatctca ctctattatg atgtgacatt tattttacaa actaatttgt
     9121 tttgaacctg aaaataactt tttacaacta aattgtcgaa aaacagtgtg tacatgatat
     9181 aataaattta taaattgaaa ataattcaaa agaaaaatta aaatatagat tgagcacgaa
     9241 gtgatttgaa ataaggttgt gaaagggaat gacaaggtca gcattaaaac catttaaaaa
     9301 taaacgcgtt atggttactg gacgtataca acgtgttttg tttaaaaatt atttagatag
     9361 acatagcaca tttaagccga atgtaaggat attattaaaa gatgtatttg tttcaggtgt
     9421 atcaatagat catttatggt tatatgagac aaataaatac tatgcattgg caatggaact
     9481 tattcatcaa cgagtaaaat ttagtgcgaa tgttgtaccg tattacaaaa taaatagaaa
     9541 taataattta ttcgtacaag attatggaat taagcgtaaa ggtaggttaa ttactgaaga
     9601 agcttacaat caaaacaatc agtatcagga taagatatat gaaaaattac cggatataga
     9661 t
//
LOCUS       DANRDI010000101         9527 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.101, whole genome shotgun sequence.
ACCESSION   DANRDI010000101 DANRDI010000000
VERSION     DANRDI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.101"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(32..547)
                     /locus_tag="PZ377_001890"
     CDS             complement(32..547)
                     /locus_tag="PZ377_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6358758.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            738..1742
                     /locus_tag="PZ377_001891"
     CDS             738..1742
                     /locus_tag="PZ377_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDM6358759.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(1788..2189)
                     /locus_tag="PZ377_001892"
     CDS             complement(1788..2189)
                     /locus_tag="PZ377_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6358760.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(2225..3259)
                     /locus_tag="PZ377_001893"
     CDS             complement(2225..3259)
                     /locus_tag="PZ377_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDM6358761.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(3270..3386)
                     /locus_tag="PZ377_001894"
     CDS             complement(3270..3386)
                     /locus_tag="PZ377_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358762.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(3491..3922)
                     /locus_tag="PZ377_001895"
     CDS             complement(3491..3922)
                     /locus_tag="PZ377_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDM6358763.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(4129..5406)
                     /locus_tag="PZ377_001896"
     CDS             complement(4129..5406)
                     /locus_tag="PZ377_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDM6358764.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            5567..6190
                     /locus_tag="PZ377_001897"
     CDS             5567..6190
                     /locus_tag="PZ377_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDM6358765.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            6236..7180
                     /locus_tag="PZ377_001898"
     CDS             6236..7180
                     /locus_tag="PZ377_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDM6358766.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(7266..8708)
                     /locus_tag="PZ377_001899"
     CDS             complement(7266..8708)
                     /locus_tag="PZ377_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDM6358767.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            8891..9274
                     /locus_tag="PZ377_001900"
     CDS             8891..9274
                     /locus_tag="PZ377_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDM6358768.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT         3230 a         1729 c         1376 g         3192 t
ORIGIN      
        1 gatttcattt tatctgctgc aaaaacaatc attataactc tttttccata attaaatctg
       61 tatccgttac atcacctgtt tgaaaatgat gttcaccaac cactttaaat ccatgacgtt
      121 tataaaatgc ttgagcacga ggattatgct cccaaactcc tagccaaatt ttatgtttat
      181 tatgttcttg agcaattttt tcggccaatt ctatcaattg tgaacctctt ccgccacctt
      241 gaaagtcttt caaaaaatat atgcgctgca cttctaaata ggtctccccc atttcttcag
      301 tttgagcact attaatattc atctttatat aaccaacatt cgcaccatct tcttgataaa
      361 aataatgaaa tgaatctaca tggttaatct cttgtgtaaa tttctctaca gtataattgt
      421 ctttaaaaaa ttgatcaaaa tctttgtcat catagtaaga accaaacgtg tcataaaatg
      481 ttctagttgc taattcaact aattcactag cattttgttc tgaaatttct ttgattatcc
      541 cagccatata aatcctccaa taaacagtga tcgaatcaaa atattactta tgttattttt
      601 cagccaaaac tatttaaaaa tacattaaca caaatcaatt acaaattgta ttgattgtgt
      661 gtaacatcaa taaatgatac atttattcca gtaaaatggc cgtattttca aaagagaaaa
      721 agagaggatg tatcgttgtg atagaaacat ttaaagcgtt tgtaattgat aaagatgaga
      781 gtggtaaagt gacaccaact ttcaaacaat tatcgcctac tgatttacct aaaggagatg
      841 tgctgattaa agtacattac tctggtataa attataaaga tgctttagcg actcaagatc
      901 ataatgcagt cgtaaaatcg tatcctatga ttccaggaat agatttagct ggaacaattg
      961 ttgaatccga agcaccaggc tttgaaaaag gagaacaagt aattgtaacg agttatgacc
     1021 taggtgtcag ccattatggc ggttttagtg aatatgcgcg tgtaaaatca gaatggatta
     1081 tcaagcttcc tgatacttta acattagaag aatcaatgat atatggcaca gctggttata
     1141 ctgccggttt agcaattgaa agacttgaaa aagttggaat gaatattgaa gatggtcctg
     1201 tactcgttcg cggtgcttca ggtggtgtcg gtactttagc agtactcatg cttaatgaac
     1261 ttggttataa agttatcgca agtacaggta aacaagatgt tagcgatcaa ttacttgaac
     1321 ttggtgccaa agaagttatc gatcgacttc ctgttgaaga tgatcataaa aagccactcg
     1381 catcatcaac ttggcaagct tgtgtagacc ctgttggtgg cgaaggtatt aattatgtta
     1441 caaagcgttt aaatcatagt gggtcaattg cagttattgg tatgactgcc ggtaatactt
     1501 atactaattc tgtattccct cacattttaa gaggtgtaaa cattttagga attgactcgg
     1561 tatttactgc tatgaaatta agacagcgcg tttggcgtcg tctcgcaaaa gatttaatgc
     1621 ctgaaaattt acatgagatc aagcaagtta ttacatttga tgaacttcca gaacaactta
     1681 acaaagtaat taaacatgaa aataaagggc gcattgttat cgatttcggt gtagataaat
     1741 agtattcatg aaaaagacat cccgttatgc gagatgtctt ttttaattta gtatttgata
     1801 tacataccgc ctgaatctgg ttcggtaggt ataaatccaa attttgtata taatttatcc
     1861 gctgggtagt ctgcaatcag actaacgtat gtactctcaa cagccacacc tttaatatat
     1921 tgcataatat gctccataat tagactgccg taaccttgac cttggtaact tttcaaaact
     1981 gcaatatcaa caatttgaaa aacagttccg ccatcgccaa tcactctacc cataccaatt
     2041 aaccgatctt tatcatacaa ggttactgta aataaggcat taggtaatcc tttttcagct
     2101 gcttcgcgcg tctttggact catacctgcg ttaatcctta atgcgcaata atcctcgcaa
     2161 gtcggaatat catatgtcac tttaaccatt atttacccca cttttcatca cacaatatat
     2221 caacctagta taaatgttta tttacaatag tcttattcgc ttctttaaac acttcatgat
     2281 gacttgaaac ataaccctct gcattcgcat ctggttggat atatgtttta gcaaggttcg
     2341 ctgcatttgc accatcacta aatgcacttg caattagatg tgattttgca tcatgataaa
     2401 caatatctcc acacgcatag ataccaggta tactagttgt cgtattacca aatcctttaa
     2461 cacgacaatc atcatgcata tctagctttg aagatgtttc actcaataat gtattacaac
     2521 gatcaaaccc atgactaata atgacatcgt caaatttaac tgtatgccta tcgccacttt
     2581 caacatgttc caaaacaact tcacttatat gcgtttcatc atcattgccg accaagtatt
     2641 taatacgtgt ttttgggcat agtttcacat ttaaatctgt caccaacgtt ttcatcgctt
     2701 catgaccact tacatcttct tttcgataaa caactgtcac gcttttagca atcttggcaa
     2761 tatcatgcgc ccaatctaat gctgtatttc ctccacctga tattaataca tctttatctt
     2821 tgaaacgtct gtaactttgt acaacataat gtaaattagt taattgatat ctctctacac
     2881 ctttaacatc taattgtttt ggattaataa tacccgcacc aattgcaatg ataactgctt
     2941 tcgatgtata tatttctccc gcttctgttt caacttcgaa atgacgttct gcctttttcc
     3001 taatatctac cacacgttca ttcaaatgaa cttccggttt aaaatataat ccttgcttaa
     3061 ttgtatcttt taaaatttca tgacaaggtt ttggcgcaat gccgccaata tcccaaataa
     3121 ttttttcagg gtaaattctc atcttacccc ctaattcaga ttgaacatct atcaatctta
     3181 cagacatatc tcgcaatcca gcataaaagc ttgcatacaa accagacgga ccgccaccaa
     3241 tgattgtaac atctttcatt atgtgcctcc tatgactctc tatattcatt tctttcatta
     3301 acgtgctcaa attgataatt attatcattt aaagccatta tactattaat atttatattg
     3361 ttaaaataaa tcgcatagtt agccatgaat tatcaataac tatcccatca caatataaaa
     3421 acacaccttt caaagtaaga aacaacaatg ttcctttgaa agatgtgtat attttttagt
     3481 tctagttata ttatttttta aaagactcat cacgtggttc tttaagaatt gcttgtctta
     3541 aaaggaaaaa tagcaacaat aaacctgcaa gcatacctgt gtgcccaata cctgcaaagc
     3601 ctgcaaatgc ttctggagag tatgatttac cagtgacttg gaagaatcct tttgtcacca
     3661 tcatacctat agtaacgata acacctatgt tatatacata gaagaaccag ttaaataagt
     3721 agtaacttga taatttaaat aacttctcca atggtaataa tattaaaaac ataaacatac
     3781 ctaaaataag tgtatgtgtg tgtacaagtg ataattgtgt tgtaccagta aaatcatatg
     3841 cttttgttag ctctctatag aaaaagccac taaataaacc tatgatcata taaaaaagaa
     3901 atgaatataa taacctacgc attttgaatc ctccctcacc ttttcattat atccgattat
     3961 acctattata acagtgacaa aaaagcgaag atatgacaca actgaatttg gtttagcaca
     4021 tagtatgaca gctaaaataa ctttacatca agcgctatac aaataaaata gctataccat
     4081 gacgctcgct acaaacgtct tgtatagcta ttatttctat ttattaaatt atgacggcac
     4141 ttcagtacgt tttgtgctat gttttctaac aatagctgaa actaaacttg atacagcgcc
     4201 aaaaatcata aaccctataa acatccacat tgtactattt aattcaatat gatgattcaa
     4261 gtataatagt tctcttacac ttgttgcata gtgtgtgaat ggattccaat catatacata
     4321 tttttgataa ctttcaggca acatttgttt aggtaacgtt acaagttgca tactaaagaa
     4381 cattaaaatg aagaaaattg gaactgactt catacctagc cataccataa cacctaaaat
     4441 aaggcccacg aaaccaagaa tcgcaaatgc tacaaaaatt gcgatacgat ttggatggtc
     4501 aaaatcaaat ctttgaacgc cttgcatgaa ataaatataa acaaagctac ctgcaaatgc
     4561 tgcaacaact gcaaatatca tctgtccaat tgaagcaatg atacgatgtt gcacgacgat
     4621 attgttacta gttctaaatg caaagaacaa taagatagac gttacgattg aacctatcca
     4681 aattggcata aacattagga atggtgcatt accacctgct tggtgatctt taactttatt
     4741 aagtttttca ttatccactt ttactggatt cgtcaaacca ttgatgtccg cggcattgac
     4801 tttaacattt tgactcgtta atgtttccaa actttgcttc gtaatttgag cattaatatt
     4861 atcacccata ccagttaaca cagctgatgc aatttgtgaa ccttgtaagc ttgatccttc
     4921 acttacaatc gttttaaatt tagcctgctt aacctctact tgttgattcc ccattttttg
     4981 tttcatttgt tgaaccactt gtggcggaat ttcacctgaa gcaacttttt gttgcatttc
     5041 ttctttttta ctatccataa ctactttttg tgttttactc atagcatctt ttgaaaagtc
     5101 tttatcaata atagctactc caaagatttt ttgatctttc aaatcttttt caaggtcttt
     5161 ttcactatca accttaaccc atttaatttt attagaatca ctatctaata atttatcctc
     5221 taatttttta ccaatgttaa catttttatc ttgaatcgtt gtacctttat cctcgtttaa
     5281 tataccaatt ggtaaatctt ttggtttagg attatatgca ggataaaaag ctaaagaaaa
     5341 gattaccaag ataatcatag ttgctattgg tgcaatccat aataatttat ttttaaatat
     5401 attcatttta ttaattacct ccttattaga caatgtgttg aatatttcgc atgttgtcta
     5461 ttataatatg taagtgggaa taaacaatac acaatgttca tcaatacgtt cattaataga
     5521 caaatacacc cacattgttc aataacacaa ggaggttgat ttatttttga aagaagatag
     5581 gcgaattaga aagacaaagt cctccattaa acaagcgttc actaaattac tacaagaaaa
     5641 agatttagaa aagattacca ttcgcgacat aacaacacgc gctgatatca atagagggac
     5701 attttactta cattacgagg ataaatatat gttactcgca gatatggaag atgagtatat
     5761 ttcagaacta acgacatata ctcaatttga tttgttacgc ggttcttcaa ttgaagacat
     5821 tgcgaatact tttgtaaata atatactcaa aaatattttc caacatattc atgataattt
     5881 agagttttat catactattt tacaattgga acgcacgagt caattagaat tgaaaatcaa
     5941 cgaacatatt aaaaataata tgcaacgtta cattagtatc aatcattcta tcggaggcgt
     6001 tccagagatg tatttttaca gttacgtttc cggagcaaca atttcaatta ttaaatactg
     6061 ggtaatggac aaacagccca tttcagttga tgaattagct aaacatgtgc ataatattat
     6121 ttttaatggt ccattaagaa taatggcaga aaaccgtttg cataaatcaa accttgattc
     6181 actaacttaa gtaatactcg cttttcctaa atcctttaga aaggaactat atatcatgat
     6241 tacttcattc agacattctg aagatataga taaacatatt ataaaaacac cattagatca
     6301 tacagcgtca tggattaatg tagtagaacc agaccgagaa gaaattgaaa atcttatgga
     6361 acaatataat atacctgaag actttatacg tgaccctttg gactcagaag aaagttcccg
     6421 tattgaatat gacgaagata ctggttactc attaatcatt attgatttac ctatcgtcaa
     6481 ttcaactaat cgtagcgttc tatcttttgt aacgattcca ttaggcatta ttattggcaa
     6541 cggtattatc gtaacagttt gtgacgctga aaatgaattt ttagaaaatt tacctaagcg
     6601 tgatattaat ttaaaatttc acagcagatt tgcattagaa attttaacta ctattgccga
     6661 ccattataat cgtaacttac gattacttaa taaaagtaga attcgtattg agaaagaact
     6721 aaaaaataac attactaaca aacaactttt caaattaatg gaagttgaaa aaagtttagt
     6781 atacttttta gctgccttaa aaggtaacga tacaattatt aaaaagttat tccgtttacc
     6841 tgcaattaaa cgttttgaag aagatgaaga attactagaa gatttaatca ttgaaaataa
     6901 ccaagccatc gaaacaacag aattacatca acgtatctta gaaagtatca caacatcata
     6961 cgcttcctta ttatctaatg atatgaatac gattatgaag acattaacac ttttcacggt
     7021 actattaacg ttaccaacac tcgtatttag tttcttcggt atgaatgtgc cgttgccaat
     7081 tgatgaccat agttatatat cttggattat tgtggtaggt atttcattaa ttcttgtcgt
     7141 aattgttagt attttcttat ggcgtaaaca gaaattataa ctaaattaaa aggtatgaca
     7201 gcttaaagtt tcatcctatt gcatttgcaa tgactgatac tttgattact gtcatacctc
     7261 tttttttatt ctacaacttc tttatttgct tttcttttag ataaaattaa agtaactgtt
     7321 atagcaatga tgaatgatat tgtcatacca acaaagtagt gtaaccatcc tgcatgtact
     7381 ggattgattg aaataaatcc tggcaatcca gcagttccta atgcgattgc tttaaccttg
     7441 aagaaagcaa tatatgctga accaatacct gatccaacga tagcgccaat aaatggatat
     7501 cttagtttta agttaacacc aaacatagcc ggttctgtaa taccaagtaa tgctgaaata
     7561 cctgcggcag atgccacacc ttttaacttc ttattttgtt taataataaa gaacgctgca
     7621 attgctgcac caccttgtgc aacattagac attgtcgcaa tcgggaatat aaatgatcca
     7681 cccgttttag tggcatctgc aattaatgtc gtttctacag ctataaagct atgatgcata
     7741 cctgtaataa caatcggagc atacaataat ccgaatatta atccaccaat tgcaccacca
     7801 aattcataaa gccatgttaa accatctgat aaccagtaac ctaattgtcg agtgattgga
     7861 cctacaaatg aaaatgttag aaatgctgtt ataaaaatag ataacaatgg tgttaacaaa
     7921 ttatctaaca ccgttggaat aactttgcgt aacccttttt caattgaggc taagatataa
     7981 gctgctacaa gcataggtaa cacttgtcct tgataaccta cttgattaat atgcaaacca
     8041 aaaacatccc aatatggaat agccttacct gcttcaactg cttttgggaa atcgtatgcg
     8101 ctcattagcg atggatgaac tagtatcata cctaatgcag cacctaaaaa tggattgcca
     8161 ccaaagcgtt ttgctgcact aaatccaatt aaaattggta ataatgtaaa tggtgcattc
     8221 gcaaaaacat ttatcatttc agctaatcca gcaaattgac tatatacatc tatcaatgat
     8281 ttacctgaaa agaataaatc tttcgcagtc aaaatgttat ttaaccccat taataaacca
     8341 ccagcaacga tggctggtat aatcggaaca aagatatctg aaagcatttt tacaaatcgc
     8401 tgtaacggat tcatattttt agcagattgt gctttgactt ccgaagtggt tgatgcttct
     8461 tttccagtta atttttccag ttcactaaat actttattga ctgtaccaga cccaataata
     8521 atttggtatt gtcccccagt agaaaacgtc cctttaacta catccatatt gtttagcgcc
     8581 tcttcattta ctaaactttc atcatttaaa actaatcgta gtctcgttgc acaatgcgcc
     8641 attgcatcta aattctcttc tccgcctatc gcgttcaaaa tttcttctgc ggattgctta
     8701 taattcatat atttagcctc ctaattagaa ccggttccat ttattccact ttattgtaac
     8761 cggttccaaa aatatttgca acactctatt atcattttat ataaacacct acaccatttt
     8821 tgatagcgca ttcattttta ttgctatact acatgcatgt atattaacat taattatcgg
     8881 aggtaatttt atgaaagaac gtggctatat cgaacagctt tggcgtgagg aaaaatatca
     8941 tgtattgtta cacagtcaac agtcttacca aatgataagg aatgctttaa aaacggattt
     9001 atcacttcat caagttcaac aaatgattga tgttgctttg ttaattgaac cgagtatagg
     9061 aagtgtatgt aatgcttttg atcatatgtg gggatattta aaaaaatgtg cgaatgaaga
     9121 agaaagacaa caatcaaaac tacttaaagc tgattttatt aatggtaaaa tcgacaccca
     9181 aactttatta gattttttag cagaacttgc taataaatat gatgttcaat atttactaca
     9241 aagtcgtgtt ttaaacacaa aaagaaagag atagattgaa actttagttg taacggacaa
     9301 atagtagtca ttggagttca gcaattcata ctttgtacta aagtttacta taatctatct
     9361 ctcaaggttt aaatgtacat atctatatta aaatgcgttt catgatgtta gaagatacca
     9421 tacacattta attagttaca gcataaatca atttataccc ttaattatta cattgtttat
     9481 attttaaatt cgggggagta attttaagta atatcttgtt gctgcta
//
LOCUS       DANRDI010000102         9510 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.102, whole genome shotgun sequence.
ACCESSION   DANRDI010000102 DANRDI010000000
VERSION     DANRDI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.102"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..625)
                     /gene="bshA"
                     /locus_tag="PZ377_001901"
     CDS             complement(<1..625)
                     /gene="bshA"
                     /locus_tag="PZ377_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDM6358769.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQV"
     gene            complement(866..1183)
                     /locus_tag="PZ377_001902"
     CDS             complement(866..1183)
                     /locus_tag="PZ377_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDM6358770.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(1520..2200)
                     /locus_tag="PZ377_001903"
     CDS             complement(1520..2200)
                     /locus_tag="PZ377_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDM6358771.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(2272..2859)
                     /locus_tag="PZ377_001904"
     CDS             complement(2272..2859)
                     /locus_tag="PZ377_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDM6358772.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(2849..3424)
                     /locus_tag="PZ377_001905"
     CDS             complement(2849..3424)
                     /locus_tag="PZ377_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDM6358773.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            complement(3438..4682)
                     /locus_tag="PZ377_001906"
     CDS             complement(3438..4682)
                     /locus_tag="PZ377_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358774.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(4689..5987)
                     /gene="aroA"
                     /locus_tag="PZ377_001907"
     CDS             complement(4689..5987)
                     /gene="aroA"
                     /locus_tag="PZ377_001907"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDM6358775.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(5997..7061)
                     /locus_tag="PZ377_001908"
     CDS             complement(5997..7061)
                     /locus_tag="PZ377_001908"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDM6358776.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(7087..8253)
                     /gene="aroC"
                     /locus_tag="PZ377_001909"
     CDS             complement(7087..8253)
                     /gene="aroC"
                     /locus_tag="PZ377_001909"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDM6358777.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
     gene            complement(8639..8839)
                     /locus_tag="PZ377_001910"
     CDS             complement(8639..8839)
                     /locus_tag="PZ377_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358778.1"
                     /translation="MKNIISIILGILMFLKLMELLYGAIFLDKPLNPITKIIFILTLI
                     YIFYVLVKELIIFLKSKYNKSA"
     gene            complement(9048..9497)
                     /gene="ndk"
                     /locus_tag="PZ377_001911"
     CDS             complement(9048..9497)
                     /gene="ndk"
                     /locus_tag="PZ377_001911"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDM6358779.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         3091 a         1753 c         1270 g         3396 t
ORIGIN      
        1 ttacttgtct aaaattcgat acatgtatta acactttttc atctggtgct ataccaaatt
       61 gtgattttaa tgctgtgtta tgtttagttg gaaactcatt ttcacgtaca aaattataaa
      121 tcggtataat ttctttgtta gtttcaataa tttcatgtgt ttcttgtgct aaagatttac
      181 tcacacttgt cacaatatca cttttttcaa tgccaaattt aattgcacct tggagtgaat
      241 gatcatagcc caaaacagta atatcagtac cgtgtagcgt tgtcataatt tttatatctt
      301 tacctgacat ctcacgagct aaaatcccac aaattgcatg aggtacagca tagtgcatat
      361 gcaacaaatc aagatcatat tctttaataa cttcagcgat tttagtactt aacgtaatat
      421 catacggtgg atactgaaat actgcatatt gattcacttc aacttgatga aaaatcatat
      481 tcggtaatgg ttttcttatt ctaaacggga tatttgaagt gataaaatgt acttcgtgac
      541 ctcgctctgc taatttaatt cctaattctg tggcaataat tccagaacca cccatggacg
      601 ggtaacatgt tatacctatc ttcattcgct tgcccatcct ttctttctat ttctctctat
      661 tattctcgat gcgtagataa ttgtttaaat ttaagtttat agtaatgttg agtttataat
      721 ttcatatatc taaaaacagg tgttgtatat ataatcattc atctagttat acttacttta
      781 aaaataatat aatttcatgc gatgcaattc attgatggat gtttttaatc ttaatcaaat
      841 ccaaataaag catatatttt taaattcact ttctttcgaa tcgattttta tctcttgtat
      901 taaacttttc cattgtttca ttaaagctct ctgtcatatc tattcccatt gaattcgcta
      961 aacataacaa cacaaataaa ttatcaccta attctgcttt aatcgtattt gcttcctctg
     1021 aatctttctt ctttttttca ccataggtat gatttatttc acgtgcaagt tcgcccactt
     1081 cttcagtcaa tctagctaag ttagctaatg gtgaaaaata tcctgtttta aattgtccaa
     1141 tatattcatc aacttcacgt tgcatttcta ccattgattt catttctacg ttctccttat
     1201 attgcatttc taatatagta tatatcaatt tgaagtctca tgcatgttta gtgcaatcta
     1261 ctgactctag ctaaactaga tgacaagttg tttattttag aagtttaata attcagttta
     1321 tataaatgta atgcattcct aactaaatta aatcaattga aattgggatt ataactttat
     1381 gatacgtacc actacaataa aataatatag tgaataatct accattagaa aaataagcac
     1441 aaaaaaacta gcaaccacac aaaaatgtga ttagctagtt aataagtgtc taatttaagt
     1501 taattgttaa tctataagat taatcacttg aacgcgcaat caaaataata cgtacaagct
     1561 ctgctacagc gactgcagtt gctgcaacat aagtcattgc tgctgcagat aatactttac
     1621 gcgcatgctt gtattctttt tcatttacaa tgttcaatgc cgtaatttgt ttcatcgctc
     1681 ttgaactcgc atcaaactca actggtaacg taacaattga gaataatacc gctaatgaca
     1741 ttaaaccagc accaatccat aaagcagttg aaccaaatgc actacctatc gctgttaaga
     1801 taatacctaa catgatgatc atataactta atgaactccc taggtttgca acaggtacta
     1861 atgctgctct gaatcttaag aaccaatatc cttggtgatc ttgaatggca tgaccaactt
     1921 cgtgggctgc aattgcagtt ccagcaactg atggtctgtc atagtttgca ggagatagtg
     1981 aaacaacttt ctttttagga tcgtaatgat ctgttaagaa tccttcacct ttaacaactt
     2041 cgacatcata aataccgttt gcatgtaaaa tttctaatgc aacttcacga cccgttttac
     2101 cactagttga tctaacttgt gaatatttct catagttaga tttaactttg tgttgtgccc
     2161 ataaaggaag caccattaat attacgaaat aaattatcat agtaaaaatt gaagacaata
     2221 aactcactct cctttataaa tattttactg tcatttgccg tttttatcaa atcatttaca
     2281 ctttaataat ttgtttaatt caatataaag caaaagtcca aaaacactta gacaacatga
     2341 taatacacca atttgccaca catgtgtagt tataaaatca taatatggaa attgaaggtg
     2401 aaaatagtca atataatcat tcaaaaacac ccaaatcatc gctacactga ttccaatcat
     2461 agaacgttta aacctaggat agaagtaaat tgcctgaaca gccattatac tgtgggaaaa
     2521 cattaatacc aaaccattta ctgtaatatc accttgttca ataataaata atatattcat
     2581 tataactgcc caaatcccat atttgaataa tgttacaaat gccagtgcat cgataatact
     2641 attttgtttt tgaattaata tcaatgagat agaaataact aagtataata ttgcagttgg
     2701 gctatctgga acaaaaatct taaaatgcca gggcgtatga cttaattgtt caccatacca
     2761 tatataacca taaatcatcc ctaatatatt acaaatgagt agcatcatta accaagaacg
     2821 ttgataaagt gtatattgcc aaaatgcttt aattgtcatc tgctaagtcc tcaaattgat
     2881 tatgtttatt tactagcttg agtgtattta aaatttgcgt tagttgataa aaacgttgct
     2941 tttcattcat ctgtaaactt aaatcaatat tgtgtaacaa gtaatctatt aataacgcat
     3001 gtttatgccg atctatagcc atactattta agtcatgaag ataagtttga taactgggcg
     3061 attccgttag ttgagcaact attaaatcat ctagcctttg ctcacgcttt gacacgtttg
     3121 cgaagtgaat ttgaatatca aaagcacagt tatgattagc gatataatca aatatttcat
     3181 ttgtattcat taactttata ttacgcttag taaattgaat tgcagaagcg tgacttccca
     3241 cttctgcaat ttctaatgtt tcatgatgat taatttttgt atctacaaaa tgaatgtttg
     3301 ccaatttcgc ctcattcact tttatatagt taagcaccca aactgcaata cgcgacttaa
     3361 atcgatattg aaaaagtaaa tattcaataa aactttcttt aatttgattg aatgtctctg
     3421 acatcaaata ccccatttta agattgcaat cttgataatt cgtcatgcca attttcgtta
     3481 cttggctcta gttccaacaa ttgatttaaa atagtaattg cttgttcctt ttgaccaatt
     3541 tcaattaaat agaaataata atcactcata aaatcaatat ttgttttcat cgttggatat
     3601 gctaattcaa agaaatgttg agcttcttta tctcgctctt cttgaccata ggcgaacgct
     3661 aaatgccaca tgaatgtagg atccaaatct tcttcatcta catatgttaa taattcaatg
     3721 attgcttcat aatcttcttc attacgatat aaatcgctta aaatcaataa aggttcttgg
     3781 taagcattat caacctctaa tgcttgtttt aacaataata caccttcatt agcatcgccg
     3841 tgttctattt ccaaacatcc agttgtatac attaactctt tataaaattg acttagtcgt
     3901 aatccttctt tacccgtttc aatggcatct ggataatttt tttcattttc atataatgat
     3961 tgtaaataca agtagccttg aatataatca ggatctttag aaagtaatgt agtcattatt
     4021 ttaattgctt cttgagtgat gtcattttta tcgtaagaaa tggctttttt gagataatct
     4081 tctgaagtca tttcatcttc attaatttca tcgtataagc gaatcgcatc actatagtta
     4141 ccactttgta aactacaatc tgccatacga gagaataagt ttacaccatt aacttgatat
     4201 tcaccagttt ctaaaacggt ttcgtattca gaggtagcac gtaaatattg accatcataa
     4261 tataacattt cagccaatgc aaaatggatt attggatcat ttggctctag ttcaagtgct
     4321 tcttgtaatt tatcaatagc aacttccatc atattaattt gttgatataa atctgcttct
     4381 aacatcaact tttcaggtga tggttcaaca taacttaaat attctaacgc ttcgtcagtt
     4441 tgattttcag acattaaacc ttcaataaaa taaatcagca attcactttc gtctggatat
     4501 ttgtgatata acacgcggaa tacttccaaa ccttgtggca ttaatccaaa attgtaaagt
     4561 gtctctccta gaataaataa tgcgtcatcg ttgtcagtag ttattgcttc attaacacga
     4621 gagtctaaat tttctagttt ttgtagattg atatcgtcta ttaatttata gatatcttcc
     4681 attttatatt atccctcatt ttctaaaagc tttagttttg gtaaaaatcc tggaaatgat
     4741 acatttacag catcaaattg tttgattttg acaggctcgc ttgaaagtag agaagcaact
     4801 gcaagcatca ttcctattcg atgatcagtt aaactatcaa ctgttgcatt tgttttaaat
     4861 tctgacggat gaataatcaa tccatcatta gttggttgta attcaaaccc taacaagttt
     4921 aacatatcag ccgttgtatc aattctattt gtttctttta cttttaattc ctcggcatct
     4981 ttaattgtac tcgtgccaac tgcttgtgta caaagtaatg ctattacagg cagttcatca
     5041 attgcttttg gaactaattc tccttcgatt gttattggtt gaagcattgg tgtgtattga
     5101 atacgaatag aagcagtagg ttcagcacca gttgtttgat tgaaaagttg gatattaccg
     5161 cccatttttt caacaatatc aataatacct gaacgtgttg gattgattcc aacattatga
     5221 attgttacat cacttcctgg tgtgataagt gctgcaacaa taaagaacgc tgcagatgaa
     5281 atatcgccag gaacatgaaa atctgcaggt ttaatgtatc gaattgcttc aggggttgta
     5341 ttaattgata acccttctgc ttcaattgga atattaaaat gtttgaacat cgtctcagta
     5401 tgatttcgac ttacatctaa ttctttaatg atggtcggtt ccttagaaaa caaacttgca
     5461 aataaaatgg cactttttac ttgtgcactt gcaacttcca tttgataatt tatacctttt
     5521 atgacagatg gcttaataat taatggtgta taattatctt caataccttc aatattcgca
     5581 tccataagtt tcaatggtct caagacacga tccattggcc ttttaccaat tgaaacatcg
     5641 ccagacaaaa cactttcaat acctaaacca cttaacaaac ctgccaataa tcgtgttgtc
     5701 gtaccagaat tacctgtata caatacttga tgtggcgtgt taaaagattg atatcctggg
     5761 gaagtcacaa ctaatttttc atcatcttct ttgatttcta cacctaacag tcggaaaatg
     5821 tccatcgtac gacgacaatc ttcgccaagt agtggcttat atatagtaga tacaccttca
     5881 gctagcgacg ccaacatgat tgcacggtgt gtcattgact tatcgcccgg cacttctatt
     5941 tcgcccttta acggacctga aatatcaatg atttgttcat ttaccatttc attcacctac
     6001 ttaaaatatg tttttaattg ttcacatgca tgttgtaatg ttagttgatc aacatgttgt
     6061 acaacgatat ctccaaattg tctaatcaag accatttgta caccttgctt atcattcttt
     6121 ttatcactta gcatatattg gtataacgtt tcaaaatcca agtcagttat catgtctaaa
     6181 ggatagccga gttgtattaa atattgaata taatgattaa tatcatgctt agaatcaaac
     6241 aaagcattcg caactataaa ttgatagata atgccaacca tcactgcatg accatgaggt
     6301 attttatgat agtattcaac agcatgacca aatgtatgac ctaaatttaa aaatttacgt
     6361 acaccttgtt ctttttcatc tgcaataaca atatccagct tcgtttcaat acctttagca
     6421 atatatttat ccataccatt taatgactgt aatatctctc tatctttaaa gtgctgttcg
     6481 atatcttgcg tcgctgattc accattcaat aacgcatgct tataaacttc tgcatagcca
     6541 cttaatattt gctcaaatgg taacgtcttt aaaaagtcta aatcataaat cacagcagtt
     6601 ggacgataaa atgcaccgat aaggttttta ccttgctttg agttaatacc cactttaccg
     6661 ccaacactag aatcatgcgc tagtatagtc gttggcactt gtataaagtg aacgcctcgt
     6721 aaaagtgtcg ccgcaataaa cccagcaaaa tcaccagttg caccaccacc aacagcaata
     6781 attgctgtat tacgagttac atgatgggat aaaatatact ctaatgtttc ttgatattgc
     6841 tcaaatgttt tcgtcttttc accagctgga ataataactt tatgtacatt ttcatatgat
     6901 aaaatatcat caaatttatc agcaaaatat tgatttacat gctcgtcaat taatataaaa
     6961 ctttgatcaa actgatcaat atacgtgcta atatggtcaa ttgcaccgtg ttcaacatat
     7021 attggataat tatttgaagg gtatgttgtt tgtaatttca tgattacacc tcaattgttc
     7081 ttgttgttaa aactcaatat ttaattgtct gcgctcaata atttgttgtt taagttgctc
     7141 aatatgattt gattggaatt cttccaataa tgcttttgct atttcaaatg ctacgacatg
     7201 ttcgcagacg atacttgctg caggaacagc acaactatca gaacgttcaa ttgttgcttt
     7261 aaagtcttct ttagtattaa tgtctactga atttaatggt ttatataacg ttggaattgg
     7321 tttcattaca ccattaacga taattggcat tccatttgac ataccgcctt ctaaaccacc
     7381 taagtgatta gatccacgat aataaccaat ttcactatta tatagaattt catcttgaat
     7441 ctcactacct ggcttttcag ctgctttaaa tccttcacca aagcttacac ctttaaaagc
     7501 atttatgctg acaacacctt gtgcaatctt accatctaac ttacgatcat aatgcacata
     7561 actacctaca ccaacaggca tattttcaac tacaacttga acgacaccgc caattgaatc
     7621 tccttcattt ttagcttcgt caattttatc tcgcattgct tgtgcgatac tgtcatcaat
     7681 tacacgaaca tcattacgat caagatttgc tttaaatgtt tctgaatcat aaaaatcttt
     7741 atctttaatt ccacctattt caacaacacg actgtatata tcgatatcta actgttgtaa
     7801 taacacttta cataaggcac cgactgcaac tcgagctgct gtttctctag cagatgatcg
     7861 ctctagcaca tttcgtaaat cacgatgatt atatttcata cctccaacca aatctgcatg
     7921 accaggtctt ggttttgtaa tagtacgttt catattttca cgttcttctt cacttattgg
     7981 agctgctccc ataatttttc tccaatgcgt aaagtcatca ttggttacaa ccatagtaat
     8041 tggactacct aatgtataac catttctaac gcctgatact atttctactg tatctttctc
     8101 aatttgcata cgacgtccac gtccgtaacc gccttgacgc ttaaacattt ctttattaat
     8161 atcctcaacc ttaatttcta tatttgcagg tacaccttca acaataactg ttaattgagg
     8221 tccatgtgat tctcctgatg ttaggtatct catacacact cgctcctttc atacttccac
     8281 gataacatta taatatgttg ttaatcttat catattcatt gtgcattcat aaatgtttgt
     8341 tttcaagctc aattaagaaa atggatgaca tctcatttta aatgtcagaa ttatcttaat
     8401 attaaacgaa ctaaaattaa ttataatata ttcgtctgta gaaaagtatc atgaatttct
     8461 aaacagttgc atcatataat cattaattaa aaatgattag ccgttttaat ataatcgtat
     8521 cgcaacttta gcaaataata tactgactac atttcttaat acaaaagaac gaactgttaa
     8581 ttattaataa agttaatcag tccgtttaaa aaaattatga tattaaaata tacatatgtt
     8641 aagcgctttt gttatacttt gacttcaaaa atataatcaa ttcttttact aatacataaa
     8701 aaatataaat gagagtcagt ataaaaataa tttttgttat aggattaagt ggtttatcta
     8761 aaaatatagc accatatagt aattccatta attttaagaa cattaaaatc cccaaaataa
     8821 tcgaaataat attcttcatt ttaacaaccc cttctctctg tttagtttca tttgataaag
     8881 aatattcctc tctttttaat acgtgttacc aaaaaactat aaaatttttc aagtaattta
     8941 ctacaacata aaaaacgggc cttgttatgt ataatcataa tgactaacca cataacaaaa
     9001 ccctttctac ctttataaat aaatcgtaaa ggtttacagt ttatatttta ttcatataac
     9061 catgcatcac gtggtgaagc atagctagta atttcatttt cattaaacca tagattaatt
     9121 tcacgttcag cagactctaa tgaatctgaa ccgtgaatga tatttctacc aacagttaaa
     9181 cctaaatcac ctctaattga tcctggtgaa gcttctgaag gattggtgct gccaataata
     9241 tgtctagata cattaactgc atcttcacct tcaactacca ttgcgaacac tggtgctgat
     9301 gtaataaatg aaattaaatc attataaaat ggtttacctt ggtgttcacc ataatgtgtt
     9361 tcagcaagtt ccattggtac ttgcattaat ttaccaccga caagttttag tccttttctt
     9421 tcaattcttg aaattacttc accaattaga tttctttgta ctgcatctgg tttaatcatt
     9481 aaaaatgtac gttccacttt atgtatcccc
//
LOCUS       DANRDI010000103         9429 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.103, whole genome shotgun sequence.
ACCESSION   DANRDI010000103 DANRDI010000000
VERSION     DANRDI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.103"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            111..1280
                     /locus_tag="PZ377_001912"
     CDS             111..1280
                     /locus_tag="PZ377_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDM6358780.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(1387..1815)
                     /locus_tag="PZ377_001913"
     CDS             complement(1387..1815)
                     /locus_tag="PZ377_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDM6358781.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(2031..2477)
                     /locus_tag="PZ377_001914"
     CDS             complement(2031..2477)
                     /locus_tag="PZ377_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDM6358782.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            2875..3240
                     /locus_tag="PZ377_001915"
     CDS             2875..3240
                     /locus_tag="PZ377_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDM6358783.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            3422..4699
                     /locus_tag="PZ377_001916"
     CDS             3422..4699
                     /locus_tag="PZ377_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDM6358784.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(5103..5459)
                     /locus_tag="PZ377_001917"
     CDS             complement(5103..5459)
                     /locus_tag="PZ377_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDM6358785.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(5724..6146)
                     /locus_tag="PZ377_001918"
     CDS             complement(5724..6146)
                     /locus_tag="PZ377_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDM6358786.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(6217..8322)
                     /gene="rsp"
                     /locus_tag="PZ377_001919"
     CDS             complement(6217..8322)
                     /gene="rsp"
                     /locus_tag="PZ377_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDM6358787.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
BASE COUNT         3258 a         1337 c         1494 g         3340 t
ORIGIN      
        1 tgaatgtttt catataaatg aaagcatttt taaataacat tgacctctaa tatataggca
       61 gagtattgat atctattaaa aaataaatga ttttgatgaa ggtgaaacgt atgtacaaaa
      121 caaaaggtgg ctttcaactt acattacaaa cattaagttt agtggttggg tttatggctt
      181 ggagtataat tgcgccatta atgcccttta ttaaacaaga tgtcaatgtt actgaaggtc
      241 aaatatcaat cattttagcg ataccagtta ttttgggatc ggtgctccgt gtgccatttg
      301 gttatttaac aaacattgtt ggcgctaaat gggtattctt tactagtttt atcgtattgt
      361 tattcccgat atttttctta agccaagcac aaacaccggg tatgttaatg gcttcaggat
      421 ttttccttgg tgtaggtggt gcaattttct cagttggtgt tacatcagtt cctaaatatt
      481 tccctaaaga aaaagtaggt ctagcaaatg gtatttatgg tatgggaaat atcggtacag
      541 cagtttcttc atttttagca ccaccgatag cgggtattat tggttggcaa acaacagtta
      601 gaagttactt aattattatc gctttatttg cattaattat gttcattttt ggtgacacac
      661 aagaacgtaa aattaaagta ccattaatgg ctcaaatgaa aacattatct aaaaactaca
      721 aattatatta cttaagttat tggtatttta ttacttttgg tgcttttgta gcatttggta
      781 ttttcttacc taactactta gtaaatcatt ttggaattga caaagtagat gctggtattc
      841 gatcaggtgt attcattgcg ctggcaacat tcttaagacc aataggtggc attttaggtg
      901 ataaatttaa tgcagttaaa gtattgatga ttgattttgt tgttatgatt atcggtgcca
      961 ttattttagg tatttcagac catatcgcat tattcactgt aggctgttta acaataagta
     1021 tttgtgcagg tattggtaac ggcttaatct tcaaattagt accatcatac ttcttaaatg
     1081 aagcgggatc cgcaaatggt atcgtatcaa tgatgggtgg tttaggagga ttcttcccac
     1141 cactagtaat cacgtacgta gctaatttaa caggatcaag tcatttagca tttattttct
     1201 tagcggtatt tggatgtatt gcattattta ccatgcgtca tttataccaa aaagaatatg
     1261 gctcattgaa aaacggttga tatgtaatac atgccattca tttagttaaa tacaaagcct
     1321 taatatcatg cgcaatattc gtagcatgac attaaggctt tagtagtgta ttcattttaa
     1381 atttaactat gaaattggaa tagatgttga tgaagtagtt tcattgcttg gtttgatttt
     1441 aggcaaggta atggttaata acccattttc aaaactagca gtaatatgtt gcttatcaac
     1501 agcttcaaaa tcaaattgac gcattaatga ttcgaagtta cgctcatcta aaatgagttg
     1561 ttcagatttg tattttgcgc ttctagtagc ttgaatagtg agcgtattat tattgaaatc
     1621 gatactaata tcttctttat ttacacctgc tagttcagct tctaagtaat ataattcgtc
     1681 tgtttcatat atattcgtta caaatgaagg tgttgaaaag taattaaata cttgtttacc
     1741 taaatcttta aatgtatcac ttggattagc gttaaaaaag ttttgattct cgaattgatt
     1801 gaagttcaat atcatcactt ccattataga aatttgtatt acaaagccat tatatgagta
     1861 gtattaaaaa agttaaagta aaaggggaga taaaaaagta ataaatgggt agatttgtgc
     1921 ctgataagga atcagggcaa aagaactgag catcgagatt tatacaagca ataaagataa
     1981 tgaataaaca tatcgatgca tacttctgcc catcacctta tgatagggag ttattttttg
     2041 tcggaatagt caaaattttt agaaacaaaa ttccgcaaat tattaattat atctgatgct
     2101 tctcgttctg aaatgttgaa ttcattaaag accttaacag aaatttcagc aagagggctt
     2161 tttattgctt taccttgttc tgttagagaa atttgtaggt ttctttcatc tttctcttca
     2221 cgtgttcgaa caacgtaatc tttcttttct aatttcttta gtaatggtgt cagtgttcca
     2281 gaatctaaga acacacgttc acctaatttt ttgatgttaa gtttttcatc attttcaatc
     2341 gccattaaaa caatgtaacc agtataggtt aaatcatatt ccttaagata gtttgtgtat
     2401 tttttaatta tttcttttga agaaacataa aataaaaaac acaactgttt gctaagatag
     2461 ctgttttcta catacatcca atcactcctt gttaaaataa acaatattat gaagtgtaat
     2521 agttgtcaag tactgatttc atcgcaatta ttgaattgtc tataattgaa tttaaatgtt
     2581 aaataatgca aaatatatcg atatcttagt attttcatcg gtaattactg atatgactga
     2641 ttttttaaat tgaaaaaaat gtaattgtat catccttaat tgttaaaagt agtcaattat
     2701 tttgatagat agtaaaataa aatttgttat taaatttttt aaaagcatcg tgtatgtaat
     2761 tcgatttcta taataatatt gagaataaaa aaatttgtta caatctggta aaaactcttt
     2821 attaaataaa ttgattcgtt catactgaat agataagcaa aaaggagtta cataatgaag
     2881 aagaaaaaaa acgcgattaa agttatcatt atattattta tttcattaat tgttgcagta
     2941 gcatttttct ttggtctgaa aacatatcaa ggccataaga atattcaact aattgacagt
     3001 tacttggaag agaaaaatct aaaagataaa attaaatcag aaaaaacaga gtatagtgct
     3061 aaaaaaggtc tcttctataa ggaagttaca tttaaagatg aacctggtgt aacttatgtt
     3121 gtgcaaccaa tcagtactaa taaaggatta tttgttgaag gctttgatac agagactaag
     3181 aaaagtttga aaactgcaaa gcataaatat ttcaatcaaa attataaacc atcaaaatag
     3241 tgaaacgtta aactggtgaa gcagtttagt gccttcaatt ttaattattt gaggtaaaca
     3301 gtttttgtat ggaaattagt gatttttgac atctataaaa gataaatatt ttaatttttt
     3361 atagataatt atcaattttc tgatataatt gagtgatgtc agatgaaggg gagtttgtac
     3421 tatggctcta ttcaagagaa aaattagcct acctatgcaa gttgttattg cattagtgtt
     3481 aggtgttgtc gtaggacttt tattatatgg acaagaaaac gtagcaaatt acattaaacc
     3541 atttggtgac gtatttttaa atttaattaa aatgatcgtt ataccagttg tattttgctc
     3601 actagcgctt tctatttcga acgttgggga atcgaaaact gtagggcgtt atggctggaa
     3661 aacaatttta tactttgaaa ttattacaac aatcgcaata ggtttaggga ttatcttcgg
     3721 taacctattt aaaccaggtg ctggattaga cccaacaaaa ttacctaaag gtgatatttc
     3781 taaatatcaa tcaactgcac atgcagcaga acaatctaca tatggaaatc attttattga
     3841 taccattgta catattattc cgacaaactt ttttgaagct ttaaataagg gtgaattatt
     3901 acctattatc ttcttcgcag tattctttgg attaggatta gctgctgtag gtaaaaaagc
     3961 agaaccagtt aaagaatttt taagcggatc gcttgaagct gtgttctgga tgattaataa
     4021 aattttaaaa ttagcaccac ttggagtgtt tgcattcatt tgtactacaa ttattacatt
     4081 tggtgcatcc gcattattac cactattaaa attagtatta gttgttgtct ttgcaatggt
     4141 gttctttgta ttcgctatac taggactagt tgcatggatg tgtggtatta atatcatgaa
     4201 tattattaga atcttgaaaa gtgaattgct tttagcattt tctacatcaa gttcggaagc
     4261 tgtacttcct gtaatgatga agaaaatgga aaacttcggt tctccaaaag aaattacttc
     4321 ttttgttata ccaattggtt atacgtttaa cttagatgga tcagcacttt atcaatctat
     4381 tgcagcatta ttcgttgcac agatgtatgg aatgcactta acattatcag agcaaattgt
     4441 gttgatgtta acattaatga ttacgtctaa aggtatggcg gcagtaccag gaacttcaat
     4501 cgttgtttta ttaacaacat taggtgccat gggcttaccg gcacaaggtt tagcattaat
     4561 tattggtgtt gaccgtatct tagatatggt acgtacatgt gtaaacgtta ttggtaatgc
     4621 attatcaaca atcgttatag ctaaatggga aaacgtatat gacaaagcaa aaggtcaaga
     4681 atatttaaaa tcaatttaaa aaatactatc tgacatttaa agccccttac aacctttggt
     4741 tgttaggggc ttttatgcat gcgctaaagc gcaggcgaac ggaagcgtag tgacctcagg
     4801 ttgttaggga cttttatgca tgcgctaaag cgcaggcgaa ccgaagcgta gtgacctcag
     4861 gttgttaggg gcttttatgc atgcgctaaa gcgcaggcga accggagcgc agtgacctca
     4921 ggttgttagg ggcttttatg catgcgcaaa gcgcaggcga acggcaagtg gagctattca
     4981 atatgaaagt atttcaaata taaaaagcag cagtaaaatg attccaaatt ggaaatcaac
     5041 ttaccgctgc ttttactcgt atataccttg atggcaacta aaatttgtaa tttaaattac
     5101 aattagtgtt tcatgtgcat tttgcccatg tcatgaattt tcatcattaa accatgtggt
     5161 acatcgtcat ggtcaacttt tttaacttta acaattttat ctgaatctgc taatttataa
     5221 acaattacaa aatcaccttt tttaacttta acatctgaat ctgaaggtaa tttaacattt
     5281 ttttcaatac tttcatcttt ttcactaatc attttttcaa tagtagtatt atctttcata
     5341 attccatagt atgtatcttt ctttccgcca gtcatcatca tgattactaa tacaatacat
     5401 acaattaaca tgatggcgat taaactaata ccaagaccaa aacctttttt ctttttcatg
     5461 gttcatcaat ctccttaaat gtagttaatt ctgttatacc taaaagtgca acagtttgca
     5521 aatgcagtat ttatattaca taaaattaat ttaaaaagac acaccctaat cacgttaaat
     5581 gtgaataaag tgtgtctaaa aaacaataca ttgcaaattg aattgtaaac atattaaaca
     5641 ctttaattat agcgagttat tttaagtatt taacattgtt atatctagaa ttcataaaag
     5701 ttatttccta tatacacttt gtgttataaa tcgattttga taggtgtatc taatgattta
     5761 tcattcatta gcttaatgga ttgcggaact actcttaaaa cacataactc tggatcttct
     5821 tttgaactga aaaagctttt gtcttgtgtt tcccatagcc aatcaatcac ttcttgttct
     5881 gtaacgattt cgattgtcgc ttccatctca acgaaactac gattagttgt gtcgttatag
     5941 cctaacaaaa catatgctgc tggattatct ttaatttctt tgaccttagc agaatggata
     6001 ttcgttttag tatataaagt aagaccatca ttataaaaga ccatatatct actattaggt
     6061 ttattattat atgcagttga taatacacca acttttgacg ttgctaacac gttttcaatt
     6121 gcttgaattg cttgtgagtt actcattaca aatcatctcc ttatgatata aatagccttt
     6181 aacgtaagaa actaaacaca agaggactgt tagtatttag cttggtttaa agcaaatata
     6241 tttaacctcg tcgtgattta acttgagttc aaatgtacga ccagtttctt cttgaactgc
     6301 aagctccgtt ttagggtaat tcgttttgtc taattcatct aaaatttctt tttctatata
     6361 aacaagttga tcgcttattg gaagcaaatg ttgaattgaa ccatgttcat gattcaatgt
     6421 tttaataatc atcaaagagt cttgaggtaa ttcattatgg aaaatgaaat cttgttttac
     6481 gtctgacata tatcgatcat taattttatt gaataataag aaatggtagt tattatcttt
     6541 acgactcaat acataattat ttgtaatttc aatatcttca ttaacaaaag gtctaaacat
     6601 tttatataaa tgcattaatg gtagggggct accatatttg ttgtacaagg aaatataatt
     6661 tgaatggtca tctaataatt gatagctaat gccaccgcca tatcgtaata atgcgattaa
     6721 gtgatacact atgtcagata attgaattgg attcttacca tcattagtat atttaaatcc
     6781 gcttacagac aggttattat atacaaattg tgttgatgat atacctgaat ctaaaataaa
     6841 tttcttatac tgttcaaaat gagtgtcagt ttgatgatag tgttggtttt tagtgataag
     6901 atgtgttgca gtttttgaat tttcaatatc aataaagtaa taatggaatt taagacgctg
     6961 catgacatca taaacttgtt ccacggttga ataagtttct aaaaagccat cgactgtcac
     7021 actatattta atttctttat ttttgttttt aattttaaga tgacataaat gaatttcttc
     7081 tgctgtcata gaattagagc aaaatacgag cataaattta actaatttct tgcttgtctt
     7141 atagtcttga ttgccttgta aaaatgttag tattaattgc ttcatcgttt cgaattgttg
     7201 tgttgattta atagcgattg ctaaacctat atttttttcg aaaagctttt caaaacatct
     7261 atttaacagg ttgtaattcg tttggctaat ctcaatatcg tgaatatcgt caatgaaaac
     7321 gacaggtttt ggtaagtgct caaagttaat atcgtaatat tcattgaaaa taaattgaaa
     7381 tagttcattg aaattactaa atcgaacaaa tactttggca cgctgacttc gatctttcgg
     7441 attaaaatca tctatatgaa tatctgttgt atttgtagcc agttgagctg ttaaatcaat
     7501 tgttgaaatt aactcagtga attgtgatac gttatcttgt tggaagtgta tttgtggtgg
     7561 ttcagtaatc ttggaaacga gtgaacggaa ctgtttagga ctaaaatgca agtaattctt
     7621 aaattgattt gcaaaatttg tatgactgtt aaaaccggcc aattcagaaa cagttgtaat
     7681 agaatgtttt gtagaaagta ataaattgat ggcgttaaca agtttaatac ttgtaaaata
     7741 atctttaaaa ttcatactca aatatcgaac aaaaagatta gaacaatatg attcagagat
     7801 gttgcaatgc atcgctatat cttttaaaga caagtgcgca tcaatattgt catgaatata
     7861 gtttacacag tctatgaaca ccggattgct taaagcaata tttggtaaat attcatgatc
     7921 tattcttata aaagcttctt taagtaaagt gtctataata ctttgaccta tatttttccc
     7981 cattaaagaa gaacttgttg gtgtactgat taaatcggca attaatgact taatataatt
     8041 acttgattgt aataaatgac gatctaaata accattaaag aaatcatcat cttgttgata
     8101 aaaataaaaa acgggtatca ttaattcgat tatattttcg ccgtggttaa tattataaat
     8161 atcaccatgg ttaattaaga taatataatt acttacgtct ttggtttcac cattaatcgt
     8221 aatattgagt ttacttgtaa gtgaaaatag caccataata ttattaatat tacgacttgg
     8281 tattgtcgat aatgtatgat gtattttaag ttggcatgtc atatttctct ctcctgccta
     8341 atatatattg aaatctatca tttaaatgta aaacatggaa atttttaaaa tcatggatag
     8401 tatacctatt atagttgaat atttttgaaa aggtaatagt gaaaaataaa agaaattaat
     8461 gaaaactcct aggaaaacgc ttgtttttat gcattgcgaa caatataaaa gtaaaattag
     8521 aaagatagta agcaaaattt ggaaattttc gatagagtct aattaaatta tttggtgcat
     8581 aattaatata gatttcaaac ctatgtattt caaaaaacaa tacatagcca ttttgaacta
     8641 acttgctaat cttttattga tattcaacta tgttataaaa aaagcaaatt gaccaaaatg
     8701 gaagtttgaa tcaaaaaaca ttaaatgtac ccatgactta aattttatcg ccattattta
     8761 agtttggaca tacacttttg tcatcaaacg ctgaaaatta gtacgattga aatcacttct
     8821 tgttataaaa attcttagta gccctgttac aaaatattcg ctacaaatta ttcaaatcaa
     8881 caacaataac catcaactaa gaaattttaa acaatctact aaaaaataaa gcgtttaaaa
     8941 gttctgttta gaaaacatca agataagtat aaacatctag aaaagtgaac atctaagaaa
     9001 atccatctag ttattgctgt aacaattaga ataatcattt gaaaaagtgc attagacaac
     9061 gaaattcacc atcaagataa tgaagcaaca agaaacagca cacatcaatg aattaacacc
     9121 ttagattcat catgagaaaa tgcatggcac aactagattt accatcaaga taattcttaa
     9181 gcagcgaaga attaacatct agaaatgcat aaacatctta gattttacat caagagatgc
     9241 gataactata aacaactaga aacgtcacca agatagtgca acaacaaaga atcagcatct
     9301 tgaaatcgca ctaacatcta agaatcaaca tcaagtatta tattaacatc atagataact
     9361 tcatcaagac aggcatccac atctaagaaa ctcatctaga atagctaaca cctaacatca
     9421 aagaatatt
//
LOCUS       DANRDI010000104         9411 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.104, whole genome shotgun sequence.
ACCESSION   DANRDI010000104 DANRDI010000000
VERSION     DANRDI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.104"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(268..1731)
                     /locus_tag="PZ377_001920"
     CDS             complement(268..1731)
                     /locus_tag="PZ377_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDM6358788.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(1749..6248)
                     /gene="gltB"
                     /locus_tag="PZ377_001921"
     CDS             complement(1749..6248)
                     /gene="gltB"
                     /locus_tag="PZ377_001921"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDM6358789.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            6429..7313
                     /locus_tag="PZ377_001922"
     CDS             6429..7313
                     /locus_tag="PZ377_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDM6358790.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            7428..8546
                     /locus_tag="PZ377_001923"
     CDS             7428..8546
                     /locus_tag="PZ377_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDM6358791.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            8543..9325
                     /locus_tag="PZ377_001924"
     CDS             8543..9325
                     /locus_tag="PZ377_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDM6358792.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
BASE COUNT         2886 a         1966 c         1401 g         3158 t
ORIGIN      
        1 aaacacaata tttatcatat atgaacgttc tgtaaaagtc aatattgttt ttaagcatta
       61 catgattacc acaaatcatt gtcttccact acagttgacc tataaacgaa tgtaaaaaat
      121 cactacttat aattaatcta tttgttgtct tacaaacctt tgtttttagg ttacaaagat
      181 ggcatgtgac gttgatagat actagatttt ttcaaactcg attcagcatg tcacaacgtc
      241 aacgtaacca ccatttccat acaaagatta tacacaaact ttactagcta aatactgatc
      301 tactgctttc gctacgcctc taccttcttt aattgcccat acaactaaac tttgaccacg
      361 tctagcatct ccagcagcaa ataccttttc attattagtt tgatagtttg tatcatccgc
      421 cacgattcga tttctatccg ttttaatgtt aaaagcattc ggtactgttg gttctgtacc
      481 ttcgaagccg attgataata atacaaggtc agcaggccaa aatctttcag gtccttcttt
      541 catgaccata ccattttcgc cttgctctaa aatttgagta tacaaaccac gtatgtgtcc
      601 tttatcgtca acatcgtaac gcattgtttg aacaccatat gcacgtggtt ccttaccaaa
      661 cttagcttcg tactcttggt gcgcatagtc cattttaaac accggcattg ctaaaggcca
      721 tgatgcattt tctgtaaatg taattgcttc tggcaatttc gtatatttat taaattgaac
      781 aatcgattta caattttctc ttaatgctgt cgctacacag tctgcccctg tatcaccagc
      841 accaatgata atgacattct tatcttttgc agttattgtt atatcatcaa tttctccatt
      901 taacaactgc gtttgttcag ttaaataatc catagcgaaa tgtataccat cacccatgcg
      961 tccttctaaa ggtaaatctc tacctttttg tgcaccagta cataatataa tggcatcata
     1021 ctcagattct aacgttgctt tatcaatatc aacgccgact tcaacaccat taatgaaagt
     1081 aatgcccgct tcttccatta acttaatacg acgtcgaacc acatctttat caagtttcat
     1141 attcggaata ccacacatta ataaaccgcc tgattctcta gcacgttcat aaatagttac
     1201 ttgatatcct agtagattaa gttcttcagc agcagctaat cctgctggac cgcttccaac
     1261 gattgccact ttttcatctc tacggcgact cggaactttc ggcgctaccc aaccattttc
     1321 aaaagcttca tcaataattg tgcgttcaat acctttaatc gcaatcgatt ctctattaat
     1381 cttcatcaca caagcacttt cgcatggtgc aggacataca cgccctgtaa agtcaggaaa
     1441 gttatttgtt tcgcttaagc gttcataagc agttttaaaa tcttgatgat acactaagtc
     1501 gttccattca ggaatgtagt ttccaattgg acaacctatt gtttccctac catactgttg
     1561 tccggtttga caaaacggcg ttccacaatc catacatcgt gcaccttgga tagaggcatc
     1621 ttctttagta aatcgttgtt gatatgcttt atgatgcttc aaacggtcta ccagtgataa
     1681 ttcacctaag tactgtttgt catacttcat aaatccttta aattcaccca tcgtatctcc
     1741 ccctttcctt aatacacaac ggctggttta tgtttagcat cgattgtttt actgtcatcg
     1801 taaaatgcag ctaacatcgc ttcatcttca ttgtcatgta atgatttgtg caaatgaatt
     1861 ttttgcatca ttaattgata atctttagga ataactttaa cgacgacatc ttcaatgcga
     1921 tcaaaatgtt ttaacacatg aatcgctctc gtactattcg tgtgtgacac atgttcttcc
     1981 agcatttgct taatgaatgc tttttcttct tggtgtttaa tctttgtaaa cgaaagcgta
     2041 tctagttgat tattttcaac aaaagcttct acatcagacg ggataacgta agcaatacca
     2101 ccactcatac cttgaccgaa gttcttacct acatcaccta aattaatgac atgtccacca
     2161 gtcatatact ctaatccatg gtcgccgata ccttcaacga caacatctac accactattt
     2221 ctaatacaga atctttctcc tgcactaccg ttaataaatg ccttaccact tgtcgcacca
     2281 tagaatgaga cgttaccagc aataatttca ttttgtcgtt cttcaaaagg tgctttgaca
     2341 atgaccgtac caccagataa tcctttacca acatagtcat tcgcatctcc agtatgatga
     2401 atcattaagc ctttcggtgc atatgctgca agactttgac cagcatgacc attcgtataa
     2461 acattaattg tattttcagg aagtcctgct tctccatatt gtttcgaaat ctcactacct
     2521 gtaataaccc ctacatcacg ttgttcatta tttactgtaa agctacctgt atagcgacgc
     2581 ccttcagcaa tatatggctt cgttacttca tataaatttg ttaaatcaaa tccatgctca
     2641 agattatgat tttgttgaat ttcttttgtg tttggcccat cgaaaggaca taacagtttt
     2701 tcaacatcaa tactagccgc tttgctattc gcttttaatg ttgatgatcg ttgtaataaa
     2761 tcagttcttc caactaagtc ttctacacgt ttcaaaccta aagatgctaa aatttctctt
     2821 aattcttgtg caataaaatg cataaaatta acaacatgat gtgctttacc tctatataaa
     2881 gcacgtaaat ctttgttttg agttgcaact cctactggac atgtatcttt atggcataca
     2941 cgcatcataa tacagcccaa caccactaat ggtgcagttg caaatccaaa ttcttccgct
     3001 ccaagcgcac atgcgtacgc tacatcttta ccagttaata acttaccgtc tgtttctaac
     3061 ttaacacgac ttcttaagtc atttagtttt aatgtttgat gtgtttctgc taaaccaatc
     3121 tcccaaggaa caccggcatg ctgaatactc gttttgggtg aagcccctgt accaccatcg
     3181 taaccactga tgacaatttt atctgcaaat gcttttgcca ccccagatgc aatggtacca
     3241 acacctgttt tcgaaactaa ttttaccgcg atatctgcat ctttattcgc atttttcaaa
     3301 tcatgtatca gttgcgctaa atcttctatt gaataaatat catgatgtgg cggtggtgaa
     3361 atcagaccga tacctggcgt tgaccctctt gtcttcgcaa tccacggata taccttagta
     3421 ccaggtaatt gaccaccttc accaggcttt gcaccttgcg caactttaat ttgaatttct
     3481 ttggcatgtt gtaaataatc actagttaca ccaaaacgcc cagaagcaac ttgtttaatc
     3541 gcacttactt tgttgcttcc atcaacttgt acttcataac gttttgcatc ttcgccacct
     3601 tcaccactat tactctttcc acctaattgg ttcatggctt gtgctaacgt ttcatgtgct
     3661 tccgctgaaa tcgatccata actcatcgcc cctgtattaa agcgtttgac aatgtcactt
     3721 accggttcaa cttggtcgat gtcaatcggt gtacatgctt taaattcaag taaatgtcta
     3781 atgtgatctg ttctattttt gttcaccgct tcagagtatg ctttaaattg cgcatagtca
     3841 ttttctttac atgcgtgctg caataagaaa atagattccg gattaaaagc atgatgttga
     3901 ccttgttgtc tccattggaa tgtactacct gatgcaagat aattatcatc actttgttga
     3961 cgtgctttat tttcagcatc aatttgatca atcgaaatac cagataactt agactgtgtc
     4021 ccagtaaaat aacgatcaat cacatcatga gacaagccaa tcgcttcaaa tatttgtgcc
     4081 ccttgataac tttgcactgt cgaaattccc atcttagcca ttactttaat gacaccttct
     4141 gacaatacat ccgtatatgt cttaacatta tcgacaacgg tgccttgtaa cccttctgtc
     4201 aatgtcagtt gttcaactgt acgttgcgct aggtatggca caattgcatt cgcgccatat
     4261 gcgagtaaac aagcaacatg atgcacttct cgtgtctcac cagatttagc gactaaactt
     4321 gtagacatac gtaaatctgc tttaataagt aattgatgca catgacttat tgcgagtaac
     4381 atcggcattg caaagccatt gctatcaact aatccactat catctaacac tagaatttga
     4441 gcgccttgct ttacagcatt cactgcttct cggcctaatg cttctaacgc atcttccaaa
     4501 tccccttcat ataccgttga taaataagtt aatttaaaat gttcctgatc aatcgctgct
     4561 aagtgtgatt cattcaatac cggccttttc aattgaatac gatctaaaac cgtttcgtca
     4621 ggtgctagta agttaccttc gccacctaaa taagaaagtt cactcgttac gattttttca
     4681 cgatacgcat caattggtgg attcgtaact tgtgcaaaca gctgtttaaa gtaattaaat
     4741 agtgattctg gtcgctcgtt caacactgca attggcgcat catatcccat tgcaccgata
     4801 ggatccttct taccttctac aagttcctga atatacttat gaatctcttc tttcgtgtat
     4861 gcaaactgac gttgtaattt aaataacgtc tcatctttcc attgcgaatc ttgatattgt
     4921 atattttcaa aatcaaagtc aactttatgg ttatcaatcc acgctttata tggtaattct
     4981 ccagcaatcg caccttttaa atcattattt tcaatgactt tattctgttt aaaatcaaca
     5041 agcaataact ttccaggatt caattgacct ttaaaagcaa cattactttc aggtacgtcc
     5101 acaacaccca cttcagatga aaagacaata aagttatctt tagtaatcgt ataacgacct
     5161 ggacgtaatc catttctatc tgtaagcgcg ccaagtttgt caccgttaca gaacgaaatc
     5221 attgtaggac catcccacgg ttccattaaa taactataaa attcataaaa cgcacgtaca
     5281 tttgcatcat tcgcttcatt atataaccaa ggttcaggta tgagtaacat cgctgccttt
     5341 tctggctcca tggctaacga taagaactct agcgcattat ctacaatggc agagtcacta
     5401 ccatcctcat cgacaatttg aaacacttta tgttgatcct cgccaaataa tgtttcgatt
     5461 aatttatgtt ggcgtgctcg catccagttt acattacctt taatcgtgtt aatctcacca
     5521 ttatgcatta acatacggtt aggatgtgcc cttttccaac tcgggaatgt attcgtacta
     5581 aatctcgaat gcactaaccc tagctttgat tgatataaat catccgataa atctgtatat
     5641 agttttttaa tttggtctga tcgtaaccaa cctttatata caattgtttt gcgtgataag
     5701 ctcgtaaaat acaattctaa atcgcactga gtcgaataga actctaattg ttttctcgct
     5761 aaaaacaaac gcttttcaac atcttcaatg tccctaatat caataaacac ttgttgaatg
     5821 actggcatcg tatctgctac atgtttagca atggcatctt tattaactgg tacattacga
     5881 taaccaagaa ttgataaccc ttcgccttca aaatattttt taaaaactac ttcatgttca
     5941 gaacctaaaa tgcgttcttt ggaaaaaaat aaccccacgg catattcacc ttcacctggg
     6001 atatcaaagt ccgttacatg ttgtttgaaa aatgcaaaag gtatttcagt cataatacct
     6061 gcgccatcac cagtgatgcc atctgcgccg accccgcccc tgtgatctaa gcgtcgcaac
     6121 atttcaagcg atttatcaat gatgtcgtga gaccttttat tatccatatt cgcataaaaa
     6181 ccaataccac acgcatcatg ttcctcacga tagtcatata agcctttaat taatttctca
     6241 ttgtgcatga tgcacacccc ttttttagaa tattctgata ttagttatta atgataatta
     6301 ctataatcta aaaaatttat cttttcaata tataaaatag aagatattca tctaaaaaat
     6361 agaacattta tcaataatat tgctataata gttacaaaac actcattttt ggaggtgcct
     6421 atatcgctat ggaaattaaa caattaagat attttatcga ggtcgctaaa agagaacata
     6481 tctccgaaac agcacttgaa ctaaacattg ctcaatcagc gataagccgt caaattacat
     6541 tattagaaca agaacttaat gtgtctttat ttaaaaagca aggacgcaat attacgctca
     6601 catctgaagg gaaattatta tttaatgaag ctctgcgtat tattgaacat ttagatagca
     6661 cgattgaaca atttcaaagc catggattaa ctaaaaataa aagtatctac attggctatg
     6721 atgagagtga cgtatcccac atgttactac cacttatcca aacgtttcat ttacaaaatg
     6781 atacacatgt cataccgagc ttattagaac atgacacaat tataaatagc gtattaaatg
     6841 ggaatataga cattggtttc acggaactta caccagaaat acgtaaacac aaacaattac
     6901 atatgctacc tttattcgaa gagcactatc atttatatgc gccttcagac gatccaatta
     6961 cgatggcgac acacccacca ttaattcagt ttgagcatag tcatatttat tgcttggcac
     7021 catttgccga aactgtaaaa aaacaattgc gcaagattac taaatcagat gtatacacaa
     7081 tcagtagcca accacttgcg cagtatctcc ttcgtcaaaa agaaggttac atcatttcat
     7141 cacaaaatat acatttacca gaatctaaat catggataga tataaaatta gatcatactg
     7201 aattaaaaag aacgatatgt gctattacaa aggagcctta tacaaagagt gatattggca
     7261 tattattaac acttattcag caattaatga ctaaaacgtc tacattccat taagtaattc
     7321 gatgctatca atgatgattt tcgttttaca cttagcagta atatctttga aaataaaaaa
     7381 ttaagggcgt ataatcacca tattaattca ttcatagaat tggggttttg tttttgaaat
     7441 atagaaattt atttacaaag ccatttaata taattttagg tttgttctgc atcgcattca
     7501 ttggtgctat tttatttaca ttcaataatg agaactttta caacaaacca attggtcaaa
     7561 ttatagatgt caaacatgtt tcttcgacac ctacaaaaga tgcacaaaat aatcgtgata
     7621 ttaaatataa aaatcaatta aaagtcaaaa tattaaatgg tcaatttgct ggagaaacga
     7681 cgacgattaa tcatcagtac gtcaaatcgc aagcagattc tgaggcattt cgcacacacg
     7741 aaaaagtatt attacatatt tctaacaagc cgagtgatgc ttatattata gaaaagaagc
     7801 gtgatacctt aacggttatc atcactggtt tgttcttatt aactgtatta ttggttggtc
     7861 gcaaagtcgg actgcaatcg attttatcgt taatcttgaa ttctattgcc atattaatcg
     7921 caatttacat tcatattcaa catagcaata ttaatttatt cttactcatg accattgcca
     7981 tgatctgttc aacaatactc actttattgc tcgttacagg ctggcatatg cgtacactca
     8041 ttaccatagc cagtacaatt atcggaactt ttttgagtat aggtttaaca gaattaatca
     8101 tatatatggc agatggtaaa ggcattaaat atgaaacgat gaacttttta tcattaccac
     8161 cgaaagatat cttcctagcc tcagtgttaa taggttcact tggcgctatt atggatgttg
     8221 caattacaat tgctagtggt atgcatgaaa ttttacagcg tacgccacac atttcaatga
     8281 ggagatgggc gcttgctggg cgaaacatcg gccaagatat tatgggaaca atgactaaca
     8341 tcttattatt ctcatattta tccggtgcac tacctatgtt tttaatcttt ttaaaaaatg
     8401 ccaatacagt gacttatact atttcgatga attggtcgtt agaaattgcg cgcgcactaa
     8461 caggtggtat aggtatcgta cttacaattc ctatcacaat attgtttatg gaaatatttg
     8521 aaacattaag gagggcgaaa caatgaatgc tgtatttata ttggcgttat tgctcctctt
     8581 tctgatgata attttcggag gtaaaaaggg attcatctct tatttaaccc tatttttaaa
     8641 tttcgctatt ttaattatta gtattgtatt gattattttt ggcgtaccga tttatgtcgt
     8701 cacattcttt ttctgcatta ttattggcgc ttgtaattta ttcgtactca atagttacaa
     8761 tgttaaaaca caagctgcat ttatcagcac tatcgttaca acaatattgt tgattacact
     8821 catttattta tcggtctact tcggtcatct acaaggcttt tcaaccgaac aacaagatga
     8881 aacatatgtg tattcaatga atattggcat tgatatggtt caatttatgg tgttcactat
     8941 tattcttgct gttattgcag ctgtgataga tttaacaatt accattagtt cacccattta
     9001 tgaattgcat caagtgaatc caacgcttac gcaatacgaa ttattccaat ctggcatgcg
     9061 tgttggtaga gaaattttag cgacatctgc gaacacgatt taccttgcct tttttggtgg
     9121 ccaacttgct ttatttattt ggtttttcaa acttaaatat tcatttggtc atattataaa
     9181 ctcgaaaata tttgcccaag aatttatttc tattatttta ggcggtattg ccgttgcaat
     9241 cagtattccg attacggctt ggattacggc atttttaata aaaagaccat ctcgacagcg
     9301 caaaaatgat gagtctatca attaatcgca actagcaata taacttaagt taagcgacct
     9361 gtggatatat gtcaattgtt cactaggtcg ctttttacta tagtaaattg t
//
LOCUS       DANRDI010000105         9088 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.105, whole genome shotgun sequence.
ACCESSION   DANRDI010000105 DANRDI010000000
VERSION     DANRDI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.105"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1140
                     /locus_tag="PZ377_001925"
     CDS             <1..1140
                     /locus_tag="PZ377_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDM6358793.1"
                     /translation="ADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIEQLDARVNVI
                     IIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKFISKSKDTRI
                     VFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKYRFNRLKLTT
                     IHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMIARFAYPKLP
                     QNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLGNVINASHLL
                     SQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMMNNQPETIAK
                     VLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            1121..2359
                     /locus_tag="PZ377_001926"
     CDS             1121..2359
                     /locus_tag="PZ377_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDM6358794.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            2349..3779
                     /locus_tag="PZ377_001927"
     CDS             2349..3779
                     /locus_tag="PZ377_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDM6358795.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            4047..4646
                     /locus_tag="PZ377_001928"
     CDS             4047..4646
                     /locus_tag="PZ377_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDM6358796.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            5014..5739
                     /gene="sasD"
                     /locus_tag="PZ377_001929"
     CDS             5014..5739
                     /gene="sasD"
                     /locus_tag="PZ377_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDM6358797.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(5931..6686)
                     /locus_tag="PZ377_001930"
     CDS             complement(5931..6686)
                     /locus_tag="PZ377_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDM6358798.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            6922..7629
                     /gene="deoD"
                     /locus_tag="PZ377_001931"
     CDS             6922..7629
                     /gene="deoD"
                     /locus_tag="PZ377_001931"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDM6358799.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            7636..8988
                     /gene="tet(38)"
                     /locus_tag="PZ377_001932"
     CDS             7636..8988
                     /gene="tet(38)"
                     /locus_tag="PZ377_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDM6358800.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         3000 a         1340 c         1586 g         3162 t
ORIGIN      
        1 gcagacaatg gtggtgcaca aacacatctc attcaactcg ccaaccattt ttgcgtacac
       61 aatgatgttt atgtcattgt aggcaatcat ggaccaatga ttgaacaact agatgcaaga
      121 gttaatgtaa ttattatcga acatttagta ggtccaattg actttaaaca agatatttta
      181 gctgtcaaag tgttagcaca gttattctcg aaaattaaac ctgatgttat ccatttacat
      241 tcttccaaag ctggaacggt cggacgaatt gcgaagttca tttcgaaatc gaaagacaca
      301 cgtatagttt ttactgcaca tggatgggct tttacagagg gtgttaaacc agctaaaaaa
      361 tttctatatt tagttatcga aaaattaatg tcacttatta cagatagcat tatttgtgtt
      421 tcagatttcg ataaacagtt agcgttaaaa tatcgattta atcgattgaa attaaccaca
      481 atacataatg gtattgcaga tgttcccgct gttaagcaaa cgctaaaaag ccaatcacat
      541 aacaatattg gcgaagtagt tggaatgttg cctaataaac aagatttaca gattaatgcc
      601 ccgacaaagc atcaatttgt tatgattgca agatttgctt atccaaaatt gccacaaaat
      661 ctaatcgcgg caatagagat attgaaatta cataacagta atcatgcgca ttttacattt
      721 ataggcgatg gacctacatt aaatgattgt cagcaacaag ttgtacaagc tgggttagaa
      781 aatgaagtca catttttggg caatgtcatt aatgcgagtc atttattatc acaatacgat
      841 acgtttattt taataagtaa gcatgaaggt ttgccaatta gcattataga agctatggct
      901 acaggtttgc ctgttatagc cagtcatgtt ggcggtattt cagaattagt agctgataat
      961 ggtatatgta tgatgaacaa ccaacccgaa actattgcta aagtcctgga aaaatattta
     1021 atagacagtg attacatcaa aatgagtaat caatctagaa aacgttattt agaatgtttt
     1081 actgaggaga aaatgattaa agaagtggaa gacgtttata atggaaaatc aacacaatag
     1141 taaattacta acattgttac ttatcggttt agcggttttt attcagcaat cttcggttat
     1201 tgccggtgtg aatgtttcta tagctgactt tatcacatta ctaatattag tttatttact
     1261 gtttttcgct aaccatttat taaaggcaaa tcatttttta cagtttttca ttattttgta
     1321 tacatatcgt atgattatta cgctttgttt gctatttttt gatgatttga tatttattac
     1381 ggttaaggaa gttcttgcat ctacagttaa atatgcattt gtagtcattt atttctattt
     1441 agggatgatc atctttaagt taggtaatag caaaaaagtg atcgttacct cttatattat
     1501 aagcagtgtg actataggtc tattttgtat tatagctggt ttgaacaagt cccctttact
     1561 aatgaaattg ttatattttg atgaaatacg ttcaaaagga ttaatgaatg accctaacta
     1621 tttcgcgatg acacagatta ttacattggt acttgcttac aagtatattc ataattacat
     1681 attcaaggtc cttgcatgtg gtattttgct atggtcttta actacaacgg ggtctaagac
     1741 tgcgtttatc atattaatcg tcttagccat ttatttcttt attaaaaagt tatttagtag
     1801 aaatgcggta agtgttgtga gtatgtcagt gattatgctg atattacttt gttttacctt
     1861 ttataatatc aactactatt tattccaatt aagcgacctt gatgccttac cgtcattaga
     1921 tcgaatggcg tctatttttg aagagggctt tgcatcatta aatgatagtg ggtctgagcg
     1981 aagtgttgta tggataaatg ccatttcagt aattaaatat acactaggtt ttggtgtcgg
     2041 attagtggat tatgtacata ttggctcgca aattaatggt attttacttg ttgcccataa
     2101 tacatatttg cagatctttg cggaatgggg cattttattc ggtgcattat ttatcatatt
     2161 tatgctttat ttactgtttg aattatttag atttaacatt tctgggaaaa atgtaacagc
     2221 aattgttgta atgttgacga tgctgattta ctttttaaca gtatcattta ataactcaag
     2281 atatgtcgct tttattttag gaattatcgt ctttattgtt caatatgaaa agatggaaag
     2341 ggatcgtaat gaagagtgat tcactaaaag aaaatattat ttatcaaggg ctataccaat
     2401 tgattagaac gatgacacca ctgattacaa tacccattat ttcacgtgca tttggtccca
     2461 gtggtgtggg tattgtttca ttttctttca atatcgtgca atactttttg atgattgcaa
     2521 gtgttggcgt tcagttatat tttaatagag ttatcgcgaa gtccgttaac gacaaacggc
     2581 aattgtcaca gcagttttgg gatatctttg tcagtaaatt atttttagcg ttaacagttt
     2641 ttgcgatgta tatggtcgta attactatat ttattgatga ttactatctt attttcctac
     2701 tacaaggaat ctatattata ggtgcagcac tcgatatttc atggttttat gctggaactg
     2761 aaaagtttaa aattcctagc ctcagtaata ttgttgcgtc tggtattgta ttaagtgtag
     2821 ttgttatttt tgtcaaagat caatcagatt tatcattgta tgtatttact attgctattg
     2881 tgacggtatt aaaccaatta cctttgttta tctatttaaa acgatacatt agctttgttt
     2941 cggttaattg gatacacgtc tggcaattgt ttcgttcgtc attagcatac ttattaccaa
     3001 atggacagct caacttatat actagtattt cttgcgttgt tcttggttta gtaggtacat
     3061 accaacaagt tggtatcttt tctaacgcat ttaatatttt aacggtcgca atcataatga
     3121 ttaatacatt tgatcttgta atgattccgc gtattaccaa aatgtctatc cagcaatcac
     3181 atagtttaac taaaacgtta gctaataata tgaatattca attgatatta acaataccta
     3241 tggtctttgg tttaattgca attatgccat cattttattt atggttcttt ggtgaggaat
     3301 tcgcatcaac tgtcccattg atgaccattt tagcgatact tgtattaatc attcctttaa
     3361 atatgttgat aagcaggcaa tatttattaa tagtgaataa aataagatta tataatgcgt
     3421 caattactat tggtgcagtg ataaacctag tattatgtat tattttgata tatttttatg
     3481 gaatttacgg tgctgctatt gcgcgtttaa ttacagagtt tttcttgctc atttggcgat
     3541 ttattgatat tactaaaatc aatgtgaagt tgaatattgt aagtacgatt caatgtgtca
     3601 ttgctgctgt tatgatgttt attgtgcttg gtgtggtcaa tcattatttg ccccctacaa
     3661 tgtacgctac gctgctatta attgcgattg gtatagtagt ttatctttta ttaatgatga
     3721 ctatgaaaaa tcaatacgta tggcaaatat tgaggcatct tcgacataaa acaatttaag
     3781 taccggtaat gctatacttt agaaaattaa gattaagaag aaaaggcaat ttcttattga
     3841 aaaatggaag ttgtcttttt taattctctt taaaagcggg aaacaaaagc agttaaatgc
     3901 ctttttgcat tcaatattaa atattatatc aatttcgaat atttaaattt tatataattg
     3961 gatataacaa ataaataata attattgcaa aacacacccg aaattaatta ttataaaagt
     4021 atattcataa aaggaggaat atacttatgg catttaaatt accaaattta ccatatgcat
     4081 atgatgcatt ggaaccatat atagatcaaa gaacaatgga gtttcatcac gacaaacatc
     4141 acaatacgta cgtgacgaaa ttaaacgcaa cagttgaagg aacagagtta gagcatcaat
     4201 cactagcgga tatgattgct aacttagaca aggtaccgga agcgatgagg atgtcagtcc
     4261 gtaataatgg cggtggtcat tttaaccatt cattattctg ggaaatacta tcacctaatt
     4321 ctgaagaaaa aggtggcgta atagatgaca tcaaagcgca gtggggcact ttagatgaat
     4381 ttaaaaatga atttgcaaat aaagcaacaa cattatttgg atcaggttgg acttggttag
     4441 ttgttaatga tggcaaatta gaaattgtga caacgccaaa ccaagataat ccattaacag
     4501 aaggcaaaac accaatctta ctatttgatg tttgggagca tgcctactat ctgaaatatc
     4561 aaaataaacg tccagactat atgactgcat tttggaacat tgttaactgg aaaaaagttg
     4621 atgaattata ccaagcagca aaataatata acttaatata attgaggtgg agcatctaca
     4681 aggtgttcca cttttttgta cttaatatct ttacttattt gtttttgtgt aatgggatag
     4741 cacgtaaaag tggaaaaata ataagataaa tttcaactcg aaatacctta ttattgttga
     4801 ctataacgat gacctaaata ttatatacat tagacaacac tattaattaa tcaacatatt
     4861 gacattaaat aaattgacaa aataagtaat tattgtgaat ctaaagtgaa atttttataa
     4921 aaaaatgtaa tgattcaaaa attttgttgc atttcttttg taatcgtatg ataatgtaaa
     4981 tgtaatcaaa ttgtaatata aggggacaag acaatgaaaa aattagcaac agtaggttct
     5041 ttaattgtaa caagcacttt agtattctca agtatgcctt ttcaaaatgc gcatgccgac
     5101 acaacttcaa tgaatgtgtc gaataaacaa agccaaaatg tacaaaatca tcgtccttat
     5161 ggcggagtag taccacaagg aatgacgcaa gcacaatata ctgaattaga gaaagcttta
     5221 ccccaattaa gcgctggcag taatatgcaa gactataata tgaaattgta tgatgcgacg
     5281 caaaatattg ctgataaata caatgtgata attacaacta atgtaggggt atttaaacca
     5341 catgctgtta gagatatgaa tggccatgcg ttacctttaa caaaagatgg caatttttat
     5401 caaacgaatg tagatgcaaa tggtgttaat catggtggta gtgaaatggt gcaaaataaa
     5461 acaggtcata tgagtcaaca aggccatatg aatcagaaca cacacatgaa ccaacagcca
     5521 cacatgcaac aaggtcatat gcaatcatca aaccatcaaa tgatgagtcc aaaagcaaat
     5581 atgcattcat caaatcatca aatgaaccaa agtaacaaaa aagttttacc agctgctggt
     5641 gaaagtatga catcaagtat tcttactgca agtattgccg cactactatt agtatctggg
     5701 ttattcttag catttagacg acgttcaaca aataaataaa cataatacga ttaataatag
     5761 aaaaatcgtg tgattatctg agcgagccta ggacataaat caatgtccta ggctcgctaa
     5821 tgttatattg gcagtagttg actgaatgaa attgcgcttg taacaagctt ttccatttct
     5881 taccaactcc ctaaatagtc atcaaaaaat ttcttatatt ttaataattt ttaataatcc
     5941 gattgtctta tacgtgtcag tgttaattca gatatttcct gtggaatata ccacttatta
     6001 atcataattg gataaggtgt ttgtgcgtac agtgtttcaa taatcagcca acaatgtgta
     6061 tcaccatcaa acacgtgact atgatttttg aagtggggcg ctttggtaat agacattttt
     6121 aaatctgatt gatatgcatt gctataaatc gtttgctcaa cgaatgtctt catgtcgtct
     6181 tcgttttgtg tattcacttt aaatgtgtca atgacattta acggtataaa ggtaaagcaa
     6241 aatgcatcag cttgcttaga atgattgtcc tttttttgat aatagcgttc cattgcaatg
     6301 acggcagaag gatggtttgc aaacaaatga tttgtatatt cactttctaa atcaacacga
     6361 taattaattg atgacataga tacgcgagct agcaatattt gatcaagtgg atgcttaaat
     6421 tgatccatac ttgaagcgtg ttgggcattt gtttgtggaa taacaaagtg tcccttccct
     6481 cttgtactct ctacgatgcc atcttcggct aacaatttta tagcttggcg caaagtcata
     6541 cgactgacat caaagcgcgc acaaagttcc ttttcagtag gtaatgcatg gccactcgga
     6601 tattttccta tttgaatttc tttatataac gtattataaa tcgttaaaaa ttttggttgt
     6661 gtttgcgtca cgtagacaac ctccataaag ttacttaatc actctcatca tacaataatt
     6721 tttactcaaa ttggaaaaat tataaaaatt aaatatagat aggctttgaa aattagtttt
     6781 atacaaggtt agtagctgta actgtaaaat gttcttaata ttgtcaaaat gtaatgcttg
     6841 aaagcgcttt taaaaaatat tattatatac atggttagac aaatagacaa atcactatac
     6901 aaatattggg aggaatattt tatgaaatca acaccacaca ttaaaccaat gaatgacgtc
     6961 gaaattgcag aaacggttct attgccagga gatccgttaa gagctaagtt cattgcagaa
     7021 acttatttgg atgatgtgga acagttcaat acagtgcgaa acatgtttgg ttttaccgga
     7081 acatataaag gtaaaaaagt ttctgtcatg ggttcaggta tgggtatgcc atctattggc
     7141 atttactctt atgaattaat tcatacattt ggttgtaaaa aattaattcg cgttggctct
     7201 tgtggcgcga tgcaagaaaa cattgattta tatgatgtga ttattgcaca aggtgcctct
     7261 actgattcaa attacgttca acaatatcaa ttaccaggtc attttgcgcc aattgcttct
     7321 tatcaattat tagaaaaagc agttgaaaca gcacgtgaca aaggtgtacg tcatcatgta
     7381 ggtaatgtgt tatcaagtga tattttctat aacgcggata caacagcgag tgaacgttgg
     7441 atgcgtatgg gtattttagg tgtagaaatg gaatcagctg cattatacat gaatgcaatt
     7501 tacgctggtg tcgaagcatt aggtgtgttc acagtgagcg atcatttaat tcatgaaacg
     7561 tcaacaacac ctgaggaaag ggaacgtgca tttacagata tgattgaaat tgcactgtca
     7621 ttggtgtaga tgattatgaa tgttgaatat tctaaaataa agaaagcagt acctatttta
     7681 ttattcttat ttgtattcag tttggttata gacaactcat ttaaattgat ttctgtagcc
     7741 attgctgatg acttaaacat atctgtaacg acagtaagtt ggcaagcgac attagccggt
     7801 ttagtaattg gtattggcgc tgtagtatac gcttcattat ctgatgccat tagtatacgc
     7861 acactattta tttatggcgt gatattaatc attatcggat caattattgg ttacattttc
     7921 caacatcaat tcccattact tttagttgga cgtattattc aaactgccgg tttagctgct
     7981 gcagagacat tatatgtgat atatgttgca aagtatcttt ctaaagagga ccagaagact
     8041 taccttggct taagtacgag cagttattcc ttgtcattag ttatcggtac attatcaggt
     8101 ggatttattt ctacgtattt acactggaca aatatgtttt taattgcatt aatcgtagta
     8161 tttacgttgc cattcctatt taaattatta ccaaaagaaa ataatacgaa taaagctcat
     8221 ttagattttg ttggcttaat tctagtggca actattgcta caacagtcat gctgtttatt
     8281 acgaacttta attggttata tatgattggt gccttaattg cgattatcgt ttttgcgcta
     8341 tatattaaaa atgcgcaacg tccattagta aataaatcat ttttccaaaa taaacgttat
     8401 gcttcatttt tatttatagt atttgtaatg tatgctatcc aattgggtta tatttttacg
     8461 ttcccattca taatggagca aatttatcat ctgcaactag acacaacatc actgttatta
     8521 gtaccgggtt atatagtagc agtcattgtt ggtgcactaa gtggtaaaat cggcgaatat
     8581 ctgaattcaa aacaagcgat tatcacagca attattttaa tagcactgag cttgatttta
     8641 cctgcatttg cagtaggtaa tcacatttca atcttcgtca tttctatgat attctttgca
     8701 ggtagctttg ctttaatgta tgcaccttta cttaacgaag ccattaaaac aatagatctt
     8761 aatatgacag gtgtggctat tggtttttat aatttaatta ttaatgtggc ggtatctgta
     8821 ggtattgcga ttgctgcggc tctaatcgat tttaaagcat taaatttccc aggcaatgat
     8881 gcattaagtt cacatttcgg tattatttta attattttag gtttaatgag tattgtcgga
     8941 ttagttttat tcgtcagctt aaatcgttgg acacaatctg aaaaataaat agatataaat
     9001 tcgcgagata tattcgtatt tatagtaaaa ttaaataaag agattatata acacgaggag
     9061 tagtaagtat gaaatttgag aaatatat
//
LOCUS       DANRDI010000106         9006 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.106, whole genome shotgun sequence.
ACCESSION   DANRDI010000106 DANRDI010000000
VERSION     DANRDI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.106"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(408..506)
                     /locus_tag="PZ377_001933"
     CDS             complement(408..506)
                     /locus_tag="PZ377_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358801.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(711..1307)
                     /gene="recR"
                     /locus_tag="PZ377_001934"
     CDS             complement(711..1307)
                     /gene="recR"
                     /locus_tag="PZ377_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDM6358802.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDKVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1314..1631)
                     /locus_tag="PZ377_001935"
     CDS             complement(1314..1631)
                     /locus_tag="PZ377_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDM6358803.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="PZ377_001936"
     CDS             complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="PZ377_001936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDM6358804.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3487..4011)
                     /locus_tag="PZ377_001937"
     CDS             complement(3487..4011)
                     /locus_tag="PZ377_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDM6358805.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4149..4250)
                     /locus_tag="PZ377_001938"
     CDS             complement(4149..4250)
                     /locus_tag="PZ377_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358806.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4319..4588)
                     /gene="ffs"
                     /locus_tag="PZ377_001939"
     ncRNA           complement(4319..4588)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PZ377_001939"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4650..5378)
                     /gene="treR"
                     /locus_tag="PZ377_001940"
     CDS             complement(4650..5378)
                     /gene="treR"
                     /locus_tag="PZ377_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDM6358807.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5403..7043)
                     /gene="treC"
                     /locus_tag="PZ377_001941"
     CDS             complement(5403..7043)
                     /gene="treC"
                     /locus_tag="PZ377_001941"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDM6358808.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7107..8534)
                     /gene="treP"
                     /locus_tag="PZ377_001942"
     CDS             complement(7107..8534)
                     /gene="treP"
                     /locus_tag="PZ377_001942"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDM6358809.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
BASE COUNT         2849 a         1962 c         1207 g         2988 t
ORIGIN      
        1 aacatttaag tcaataactt tttaaaatta attttcgtta gtaattcaag ttatttctta
       61 agttgagttg ttttgtttgc ctcaacaaat aaatactata caggctttca aactgcttaa
      121 caacagtaaa aattacacct ttgtcatctg tactgaacgc ccttatccta aaaatcgaac
      181 ttaaaaaatc cccacttata aatagtacgt tttttagaac aagaaacaga tttttctcta
      241 accatatcaa tcaaaaaaaa cgcaaaacag aacgttattt ttttaccaga aattaaaaat
      301 aaatttacaa gttttaaata atctctcaaa acaccgctat acaggctgat ttaataagct
      361 ttagaccaaa gaagtacaac ttactaagcc agcatctatc atttcaatta caaaagaatt
      421 tcatttgcat agtcattaac cgcatttatc aaattaatag caccaacaca ccataaccat
      481 agatctaatt cgataatcca attcacatca ttactattag aagtaacaaa tcacaaccaa
      541 cggcttgctt cctaaagatt acccatccct cagtaaaact aggtaaacca taccaccgcc
      601 acaccgcaaa taaagtagta catcacacca caccataaaa aaagccacaa tgattacact
      661 gtgacttttc ttactatagt attaaaatca aaaatgttta atagaagaca ttacatttct
      721 gttctacctg cgattgcttt agataatgtt acttcgtcag catactctaa atcgccacct
      781 accgataacc cttgtgctaa tctcgtcact ttgataccta taggcttaac taatctagaa
      841 atatacatgg ctgtagattc cccctctaag ttcgggttca tagctaagat taattcgcta
      901 actttatcgt ttttcaagcg ttcaatcaat gaaggaatat taatatcttc tggtccaatg
      961 ccatccatag gcgaaataga cccatgtaaa acgtgatata aacctttgta ttctctcatt
     1021 ttttccatag ctatgacatc tttgtcatct tccacaacac aaataactga acgatctctt
     1081 tgcttatctt cacaaatata acatggatca ttttcagtaa tgtgaccaca tacgctacaa
     1141 tatgttaatt ctctcttaac atctactaat gctttggcaa actgaacaac atcgtcttct
     1201 ttcatatcta aggtatgaaa agccagacgt tgggctgtct ttggaccaat gcctggcaat
     1261 ttcataaagc tatcaataag ttttgatata ggttctggat aatgcatcta tgatcacatt
     1321 ccagggatgt ttaagccttg agtatgttta cctaaacgtt cttgagtaag ctcatcagct
     1381 ttattcatcg cttcattagt agctgctaac actaagtctt gtagcatttc aatatcgtct
     1441 gggtctacag cttcttcttt gatttcaacg tcgacaactt ctttatgacc agttacagta
     1501 actgcaacca tgccaccgcc agctgttcct acaatacgct cttctttaag tttttcttgt
     1561 tcttgagcca ttttcttttg cattttttgc atttgtttca tcatttgttg catgtttccg
     1621 ccaccgcgca tattccattt cctccttgaa aatcaataaa tatttatcaa taaaatgatg
     1681 tttctttcat tatacatacg attatatcgc ttgtcatgta tcactcttca tctatcacat
     1741 gtacagtttc ttcaccgaaa agatcttttg ctttttgagc aatatctgtt tgttgtgctt
     1801 gttgctttgg catatcatcg ccttcgtttt tacgattttg taaatattcc gttcgaactc
     1861 tttgccattg atctgatggt acaccaacaa ctttaacgtt tttattaacg atattacata
     1921 caacactttc tatactacta cgtttctcgt cgtctttatt gacgatttca caatggatct
     1981 cttcctcaaa tttcacaagt acgtgatctt cacttgccgc cacaggttcc gaattttgca
     2041 ataaactaac gagtgatttt ttatcattat ttttggcatg atcaatcact tcttgccaat
     2101 gatctttcaa caatttgata tctgccttat tcgctttatc tagcactttt gcaatttgtt
     2161 gcattgaaaa tgcattttta gatttttgta tgcctctcgc aggctttttc gaagattttt
     2221 gaacaggagc gacactcact ccttgtgctt ttagtgtttt tagttcttgc tctaactgtt
     2281 ccatacgttg caacaataca tctgtgtttg gcgatgaagc aatttgtgct ggttcagcta
     2341 cattcgcaat cacttgtggt tgacccttaa tctgctcagc taattttact aacaacactt
     2401 caaaatgaac gttttgattc acactaaaac gaatcgacac taatgtatca ttaataagat
     2461 caatcatttg atataacata tctaattcta agttcatcag tgctcgatac tcagtatctt
     2521 tctcagatgt tttattcata atcgtatctc tgacaaaata aatcatatca tttattaggc
     2581 gattcacttc tttaccttct gttataaact gatggtattt tttaaaagat gcttgtacgt
     2641 caccttgtac aatatcatca aacaagtgat ccaacgcttc atcatgtacg ctacctgtga
     2701 cattcaacgc atcttgcaat gttaacgtac catcaccaaa tgcaatagcc tgatccataa
     2761 tacttaatgc atcacgcata cccccttcag acgctttagc gataaatgcc aaggcttcat
     2821 cttcacattc aatttgttgt gcatctgcta caaattttaa acgttcaaca atttgatcta
     2881 ggctaattgc tttaaaatca aaacgttgtg ccctagaaat gattgttgga gggattttat
     2941 gtggttctgt cgttgccaat ataaaaatag cgtgtgctgg aggttcttct aacgtcttta
     3001 aaagggcatt aaaagcacct gttgttagca tgtgcacctc atctataata taaactttat
     3061 atttcgattc acttggtgca tatttaactt tgtctctaat atttcttatt tcatcaaccc
     3121 cattattact agcagcatca atttctatca catctgaatt agtcccctgc gtaatgcctt
     3181 tacaaatatg acattcatta caaggttctc catcagtgct atttagacag ttgattgctt
     3241 tagcaaacac tttggcaata ctcgttttcc ccgtacctct cggaccacta aaaatataag
     3301 catgcgactg tttttctttc gaaatcgcat tgcgcaatgt cttcgtgaca tgttcttgtc
     3361 cgacgacatc ctcgaaactt tggggtctgt acatacgata taaggcttga taattcaagt
     3421 tagcacctcc ataaacaatt acctctcatt atagcatgat aataccttta cttcttaatt
     3481 gaacaattaa taaaaatgtt ctggaaattt tacgattccg tgtggtgcat ccgtcaaatt
     3541 atcccataaa tattttacaa gtaacggtgc atcaccagat tctaatttaa tgtcatgctc
     3601 agcagcattt tgataaccca acttttcaaa gtagtcaaaa caatggtcta caacaaccgt
     3661 actatactct tgtgctttgg cacgctcttc tactgcttga accaagccac gacctaattt
     3721 ttgtccacgt aattcaggat gaactgataa agaggcaatc gccaaaccat aatacgtctt
     3781 atcatcacta ttaatttcta cttcaattaa taaaacgtgt ccaacgacat cgttattttc
     3841 atttttcgct attacttcta attcaaaatt atagcaagga gattttctta aatgttttac
     3901 tttcgcacgt gcttgccaac tcgtttcagg attatcatca aaactttctt caatactatt
     3961 taaagattta tcataatcta actctgttaa agtacttaaa tatatttgca tatgtcctcc
     4021 gtaggcattt gattgtcaat aatcataacg tatctcatta ataattctat tgtaagatac
     4081 tccccctcat ttcaccatca tttctttatc atcaaattat aacttcttac ttttcattga
     4141 cacaaaaatc attcaaactg cacatcgagt tcacttgaat caaacttcac atataaaaaa
     4201 gctacttccc acaaacatgt tccacgtata atacgctgaa ttgtcttcaa gaaagtagct
     4261 tctataatta tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc
     4321 gtgcacctaa gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg
     4381 ctaccctacg gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt
     4441 tcatgggttc atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt
     4501 cgcaaaaata cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag
     4561 ggaaccgcta cctccccacc tagcacggca agatatatat tactatattt taatagttaa
     4621 ttgcaagtat aaatcattta tatcattgtt tactttatac gacgtcttga gaagtcatta
     4681 aatttaaatt catttgcaag atgttttgaa atattatatt gaaacggcat tgtattttct
     4741 aaatacacaa tacttcgaac tgttgctgaa taagccaccg atacatcacc aaacaattga
     4801 tatgcttgtt catcaaacgg ttcaaaagta atagacttac ttgaataact aatattaaga
     4861 tttaatacct tttccaaata atcataaata gaatcactcg caacatcatt acctatatct
     4921 gaaacaatcg actttagaaa ataatcttca tcaacaatct tcacatgttg gttaagccga
     4981 cgagttctaa caatatgaat gagtgattca ctagaattga tgtttaaagc atgttgaact
     5041 tctggaactt catgcgcttc aacaacctca ttcacaacaa cttcagttaa atatgcgacg
     5101 cccatttctt cttgcatttc tttaaaacta acaagttcag aaaatggaaa ctctgtaacc
     5161 tcctgataaa tgacaagtga ccctttacca tgaatctttt gaatcatgcc gtctaatgcc
     5221 aacaaatcta atgccttacg cacggtctct cgagatgact ggtacaattg caccaaatca
     5281 tgttcagacg gaatttgttc accatattga atctgcccgt ttaatatatc ttctttcaac
     5341 gcctcataaa tcttcataaa ctttttttgt ttcgccatat aaacgcatcc actcaattta
     5401 ttttacgcgt ttgtttcgat tacaattgca ccaaacccgc ttatcgtatt attttccact
     5461 gtgcctgttt gaatcacaac acaaccttct ctagccaatc cttctggcaa atcaacagcc
     5521 gatgctgaga aattcgcaat tactagccat tgttgattct tataatgacg ttcataaaca
     5581 aataaatgat catgatccat aaaacgtggc acaatgtctc catacgtaat aatatcatgc
     5641 gtatgtctta attgtattaa tttttgatac gtatagaaaa tagactcttt attctgaagt
     5701 gcttgtctaa cgttgacctg atgataattt tccgaaatat caatccaagg cttaccagct
     5761 gtaaatccag cattaacatc atcactccat tgcataggcg ttctcgaatt gtctcgtgac
     5821 ttctgtccta aaatcgctaa cacatccgct tcagcatgcc cttcacttaa caactgatgg
     5881 taggcattaa tcgattcaac atcacgatat tgtgctattg atgtaaaatg tgggtccgtc
     5941 ataccaattt cttcaccttg gtaaatatat ggcgtccctt gcaacatatg cagtgcgata
     6001 gctaacattt tagcactttg tatcctcatc tcttccgacg tatcatcacc aaatctagac
     6061 actacccgtg gctgatcatg attacaccag aaaatcgcgt tccatccgcc accgtcataa
     6121 atacctcgtt gccattgcat cagaatttcc tttaacttat gaaaatcaag cttcgcattt
     6181 gtccactttt caccatcaac ataatcaacc tttagatgat gaaaattaaa aacactattc
     6241 aattcttggc gttctggttg tgtatactta atacaatttt caatcgtcgt cgaagacatt
     6301 tctcctatag tcatcatgtc agtgttacca aacgtttgac gatttaattc atgcagaaac
     6361 tcatgcactc taggaccatc cgtataaaat tctttaccta ttttgtcaga gtccttaaat
     6421 tcacctttag aaattaagtt aatgacatca aatcgaaaac catcaacgcc gaagtctatc
     6481 caatgattga ctatgcgata taacgattga cgtacttccg gattatccca atttaaatca
     6541 gcttgactga catcaaataa atgtaaataa tattcatctg tctcagaatc atacttccat
     6601 gcattaccac cgaatttaga ataccaattt gttggcggcc cgtcttcaga tgatctgaaa
     6661 aagtaataat ctctataagg gttatcttta gatttacggg cttctttaaa ccattcatgc
     6721 tccgtcgacg tatgattaat gacaatatct aacatcactt tcaagtcttt ttgatgcgca
     6781 actttgatta acttttcaaa atcatccatc gttccaaagt cttcattgat ttctaaataa
     6841 ttgctgatat catagccatt atcattcatc ggtgattcat acactggtgt taaccaaata
     6901 taatcaacac ccaataactt gatataatcc aatttttcta taattccatt gatatctcct
     6961 ataccattcc ccgtcgtatc attaaacgac ttaggataaa tttgatatac aacggatttt
     7021 ctccaatcta tttctttcga cacacaatta ccaccttata acgtaataaa ggacgtccaa
     7081 ataacgaacg cccctttttt attttattaa tcttcaacaa tttctttcgc tttttgttta
     7141 ctaaaatgag acatcacaat tgttagtata catggtacaa caatagcaat agctgtcaca
     7201 ataagatata ctggccaaaa ttctttttga attgaaatga atgctggcac gccaccaaca
     7261 ccaacttttc caagtacgtt attcatacca acgattgccc ccaatacaca agacgttgat
     7321 atcgcagcga taaatggata tttcagaggt aagttcacac cgaacatggc tggttcagta
     7381 acacctaaca taccagaaat acaagatgtt aatgccaagc cttcttcttt aaccatttta
     7441 cgacgtttat agacaaacca tgctccaaat gctgcagagc cctgacaaat attggaaatc
     7501 gcaacaattg gccataaata cgtaccgcct aagctgctac ccatcaattg gaaatctact
     7561 gctaaaaaca tatggtgtag tcctgtaatt acaagtggtg catataacaa tccatatatt
     7621 gctccgccaa gccatcctgc atgttggaat ataaatgtaa caccagatgt aatacctgta
     7681 ccaatcaata acgcaactgg tccaatgata ataaatgcta aaaatccagt aactaaaagc
     7741 gctacgggtc caacgaccaa catttttatc gaatcgtgaa cgactttatt taatcctttt
     7801 tcaattttag ctagaacgta agctgcaatt aaaactggca acacttgacc ttggtaattc
     7861 aactgcttaa tctctaagcc aaataagttc cacgtcggaa tattcccttt tgccaaatca
     7921 tactgagata ctaattgcgg atgcattaaa atcaaaccta agactatgcc tagaatcgga
     7981 ctaccaccaa atacacgcat actactccaa ccaattaatg ctggtaagaa aataaatgcc
     8041 gtactcgcaa tcacattaat gatgtttgaa atatcagcaa tttgtggata catctcaata
     8101 agtgcttttg gaccaaataa acctttcatt gtaagtaaat tattgattcc cattaacaaa
     8161 ccagctgtca caatcgcagg taatattggt ataaaaatat cccccaacaa tttgatcaaa
     8221 cgttgtactg gattcccttt ttgtgcagct gcttgtttcg cttcatcttt cgaagcttct
     8281 tgagcacctg tttcatcaat aaactgctta tacacttcat cgactgttcc tggaccaatg
     8341 acaatttgat attgatggtc tgctttaaac tgccccttga ccaacgcgtt attacttaat
     8401 gcgtctttat caactttact ttcatccttc agcactaaac gtaatcgtgt tacacaatgc
     8461 gttgcagctt caagattttc ttttccccca atagcggtta cgatggcttt tacatcttct
     8521 ctttttacag ccatatattt cactccctta atgtcttaaa acgagtataa cttgtctaga
     8581 caagttatgt aaaggctttc aacaaaatat ttttattttg tcacaattat ctggggtagc
     8641 catttcagaa aatcaatgag ccataacgac atatgtagca ataactcctc gctacaattg
     8701 cgttattaac aatacctcac cacgctctca ccattataaa gtgtagatgc atggccaact
     8761 cctaaattga aaaatgcata tctataaata gcaaacttgt ccacacaagc catccatcat
     8821 atttcacaaa acaaaaaaga catttagcta tgtatattgc acaagccaaa tgcccttcca
     8881 atctcaatgc cttatcattt ctattatcaa aaattacgag ccattcaaag acaaataaaa
     8941 aaggtaaata ctcaaaattt gagcacttac cttcattata tatagaattt aatataaata
     9001 ttgatg
//
LOCUS       DANRDI010000107         8976 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.107, whole genome shotgun sequence.
ACCESSION   DANRDI010000107 DANRDI010000000
VERSION     DANRDI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.107"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(269..634)
                     /locus_tag="PZ377_001943"
     CDS             complement(269..634)
                     /locus_tag="PZ377_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDM6358810.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(776..1198)
                     /locus_tag="PZ377_001944"
     CDS             complement(776..1198)
                     /locus_tag="PZ377_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDM6358811.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            1332..2072
                     /locus_tag="PZ377_001945"
     CDS             1332..2072
                     /locus_tag="PZ377_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358812.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            2065..3288
                     /locus_tag="PZ377_001946"
     CDS             2065..3288
                     /locus_tag="PZ377_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358813.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFVVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(3412..3915)
                     /gene="traP"
                     /locus_tag="PZ377_001947"
     CDS             complement(3412..3915)
                     /gene="traP"
                     /locus_tag="PZ377_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDM6358814.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            4078..4188
                     /locus_tag="PZ377_001948"
     CDS             4078..4188
                     /locus_tag="PZ377_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358815.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            4178..5215
                     /gene="hemE"
                     /locus_tag="PZ377_001949"
     CDS             4178..5215
                     /gene="hemE"
                     /locus_tag="PZ377_001949"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDM6358816.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTIINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            5273..6196
                     /gene="hemH"
                     /locus_tag="PZ377_001950"
     CDS             5273..6196
                     /gene="hemH"
                     /locus_tag="PZ377_001950"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDM6358817.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            6220..7620
                     /locus_tag="PZ377_001951"
     CDS             6220..7620
                     /locus_tag="PZ377_001951"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDM6358818.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            7943..8497
                     /locus_tag="PZ377_001952"
     CDS             7943..8497
                     /locus_tag="PZ377_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDM6358819.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
BASE COUNT         3116 a         1259 c         1648 g         2953 t
ORIGIN      
        1 aatgatttaa gtaaagcgta atcaaagtgc aaggataaca caatgaatcc taagcaatgt
       61 tttcaatcaa ttcataaata tgatttaagc gaaaatcact acaaacaatg tcttcctata
      121 atttgtaaac ataagttcat ttgagtttta aaaaagagat gacaagaaat atctaatttc
      181 taatgtcatc tcgtttattt ggcatttaat tttaaaaaat aaatatgctg ttactaataa
      241 aaatagttgt gatcgagaac gtaacctgct acttagaaat ttcgtttcca atatcctcgc
      301 cacgattttg taaattttcg atgtgtgact gtaaacgttc aatattagga ttaatgtcag
      361 atttgaagtt tccaagtaat gacttaattt caccaccaat tgaactacca aattccgcac
      421 tttcgttttt aatttgaaca ccatagttta aaatgtcatt aaaactttgt ttaatcgttt
      481 caaattcacg ttgtagttct gattttgaac cagtaggggc agtacgatcg atcgtgttgt
      541 tcttgacact tttgtcgtca cgtccttgaa gtgcaactac aaaaccagct gctacgccaa
      601 caccgatacc gaatagaatg cgtgatgctt tcatttatat tctcctcact tttaacatct
      661 gttatcttga taactcattt tgtttaaaat tgctaacctt gatgttatca cttcaaacgc
      721 aattgttata atattaatca tattataccc gttcgagctt agattaagca tacatttaaa
      781 attgtgcttg aatttgttca gcaatttgtt gttttgcatc gttatctaaa atgtcttcat
      841 gtgtttccca cttataacca aatccatcaa tatcattttc gtatcgagga attaagtgga
      901 aatgaatatg aaatacagat tgatctgcaa actcaccatt attttgaata atgtttaaac
      961 catcaggatt aaatgcacgc ttaatagcat ttgctacttt aggtaatgct gcaccgatat
     1021 gtttcattgt ttcttcatca gtttcaaaga tattagcaga agctttttta ggaattaata
     1081 acgtatgtcc tttagtaact tgtgatatat ctaaaaaggc atagacataa tcgtcttcat
     1141 atactttaaa gctaggaatt tctccagtta aaattttgcc gaaaattgtt tctgacatga
     1201 tgactacact ccttttcaac tagtattacc attcattata acattgtcgc taaaaattcg
     1261 atattaggac atgtgtattt aatatgagca ttttataatt tttggtacaa tataagtcat
     1321 ggaggtgcct tatgacagtt aaagtagaac agcttacagg tggatatgga aaacgccctg
     1381 taattaaaga tataaatttt gaattaaaca aaggcgaaat tgttggactt attggcctta
     1441 atggcgctgg taagagtaca acgattaaac atatgctagg attgcttaca cctatggaag
     1501 gatctctatc aatttcagat atcaacatta atgatgacat agaggcttat agaagaaagt
     1561 tatcttatat tccggaatca ccggttattt atgaagaact cacattagag gaacacattg
     1621 agatgacagc aatggcatat gatattgatc gtgatgaaac gatgaatcga gcaatgccat
     1681 tattaaagac attccgttta gaaaatgagt tgaaagtttt tccgagtcat ttttctaagg
     1741 ggatgaaaca aaaagtcatg attatttgtg cgtttatcgt taatcccgaa ttgtatatta
     1801 tagacgaacc attccttggc ctagatccat taggaattca atccatgtta gatttaatgg
     1861 ttgaaaagaa aaacgaaggt agaaccgttc taatgagtac acatatttta gcaacagctg
     1921 aacgttactg tgatcgtttt atcatactag acgaaggtga agtcgttgca tttggagatt
     1981 tagaagcatt gagacaacaa actggcttac ataatcaaac attagacgat atatatattc
     2041 atgtgacgca aggtggagat gtacatgcgt aaccaagcta ccacattatt taataaaaga
     2101 ttacatgccc ttcgtaaaga gaaaaattat tataataagt ttatatttaa tggtcatttc
     2161 gtggtctttt tacttatttt gctgggtgca tttatttttg gatatggtga atggttaaaa
     2221 catattccaa cgaatatcaa tttttcatta atagcagcag tgattgttgc gttgacatct
     2281 atttttccaa tgcgaccact gttaaaagaa gcggataaaa tctttttatt accatttgaa
     2341 aaacacatgt ctcaatttat gcgtcatgcg attttatata gttactttgc gcgcatatta
     2401 atccaactca ttattgttat tgtaatgttc ccgttgtttt acaatattaa tcaacataat
     2461 gtggcatttt atatttgttt tggagtcagt gcattaattt ttccatatgt tggtttacgc
     2521 ttaagatggc aatggtatca atcgggactt aaaacatggc aagttaattt aatatcattc
     2581 atcacctttg ctctaacata ttacttacta ttagctccaa aatggtacat cgcatttgtg
     2641 atggttgctt tgcctgtgtt aatagaattt ttagttaaaa aatataaacc aggctttttg
     2701 tatccttggg agaaaatgat tgcaatcgaa catcgacatc atatgaatta ttataaattt
     2761 gttaatatgt tcaccgatgt aaaacatttg aaagaatctg cagtcagaag aagttatcta
     2821 gatatacttt taccagttcc aaagggtagt aaatttaact ctaatgcgat gtatttattc
     2881 ttatttatta gaagctttat aagagggaga gacgcattta atattatctt taggttagta
     2941 attattgcag ttttattaat ggtttggtta tcatatccat tagtgacagc aattattggg
     3001 tgtttatttg tatatattat attgttacaa atggcacaat tttattcaca gcaagcatat
     3061 ggcttatggc ctcaagtgtg gcctgtacct gaagagaagg taatcaaagg gtatgaacag
     3121 tttttatata gattaatgtt tgttatttgt actgtgtttg cagtgacatt tattataaaa
     3181 catatgacgt tattctatgt tgtactcatt ttctatattg taggtttatt aacgattaga
     3241 agtattatta aaaagttgaa atatcaggaa acattattac gagactaaaa aacttccatt
     3301 ggcatgtatg ttgtaaaggt gcatgtaatg ttgaacgcca aatgatacgg cgttcagatt
     3361 acattagcat ctatacgtta acagcataac caatggaagt tttcttcgaa tctattcttt
     3421 tattgggtat agatatcttt caaaataact tgaatgttgt ccgcttgaac caaagtaatg
     3481 acttaatgcg tcttttgaaa aatggtcatt aaaggcatca gattgcttaa agtcttcgta
     3541 tgcatgtcga tcagcaaatc cgaaataaat tttgtatgtt gtacctttag caggtcttaa
     3601 caaacgatag cttttaaagc caccaaagtt tctgaaatta tcgtctacac taatcagttt
     3661 cttttcaagt tgatatgcat gatcttctgt tgatggaatg aagattgcac aatagaaatg
     3721 atgttcactg aattcaccaa tttctttaat aacttcatat attgaaggtg attttaaaac
     3781 agtctcacca tcagtttctt caaaaataac tgaagtatct gatgctgaaa attggaatag
     3841 ttgatgggtc gggttattga tttttatctg atgtaaaaat ccataagtgc cataagatgt
     3901 atatagtttc ttcatatgaa aggtccccct tatgttttta tttattatac gatgataaac
     3961 tagtcattac cactattaat aattgattaa aattactata atgaatcttg cgagatattt
     4021 agaagtaatg atacaaaata atggacttta gaatttaaat ttacaataaa tagttctatg
     4081 attaaatgtc agttttatga catttattta ttgaaaatac gaacgaatga gcgatatgat
     4141 aatatagata agaatgattt taatttagga ggcctttatg gtgcataata aaaacaatac
     4201 aattttaaaa atgatcaaag gtgaagaaac atcacataca cctgtttggt ttatgcgaca
     4261 agctggccgt tcgcaaccag aatatcgaaa attgaaagaa aaatattcac tattcgatat
     4321 tacacatcag ccggagttgt gcgcttatgt aacacattta ccagttgata attatcatac
     4381 agatgcagca attttataca aagatattat gacaccatta aagccaattg gtgtcgatgt
     4441 agaaattaaa tcgggtattg gtccagtgat tcataatcca atcaaaacaa ttcaagatgt
     4501 tgagaaactt tctcaaatag accccgaacg agatgtacca tatgtattag atacaattaa
     4561 acttttaaca gaagaaaagt taaatgtgcc gctaatagga tttactgggg caccatttac
     4621 attagcgtca tatatgattg aaggcggacc atcgaaaaat tacaatttta caaaagcgat
     4681 gatgtataga gatgaagcaa catggtttgc tttaatgaat catttagttg atgtatctgt
     4741 taaatatgta acagctcaag tcgaagcagg tgccgaattg attcaaattt tcgattcatg
     4801 ggtaggtgca ttaaatgtcg aggattacag acgttacatt aaaccacata tgattcgatt
     4861 aatcagtgag gttaaagaaa aacatgatgt gccggtaatt ttattcggtg taggtgccag
     4921 tcatttaatc aatgaatgga atgatttacc gattgatgta ttaggcctag attggagaac
     4981 gattattaat caggctcaac aattaggcgt tactaaaaca ttacaaggga atttagatcc
     5041 atcaatttta ttagcaccat ggaatgtcat tgaagagaga ttgaaaccaa tattagatca
     5101 aggtatggag aacggtaaac acatctttaa tttaggacac ggtgtattcc cagaagtgca
     5161 accagagacg ttacgtaaag taagcgaatt cgtacacaca tatacacaaa gataatcata
     5221 gttttacatt gaagcacatg aaagataatt catttaaagg aaggtattat caatgactaa
     5281 aaaaatggga ttattagtta tggcttatgg cacaccttat aaagaaagtg acatagagcc
     5341 atattataca gatattagac atggtaaacg tccatctgaa gaagaacttc aagatttgaa
     5401 agatagatat gaatttatag gtggtttatc accattagca ggtacaacag atgaccaggc
     5461 tgatgcgcta gtttcagcat taaataaagc atatgcagat gttgaattta aactatactt
     5521 aggattaaaa cacatttcac catttatcga agatgcggtt gaacaaatgc acaatgatgg
     5581 cattactgaa gcaatcacgg tagtactagc accacattat tcttcatttt cagtaggatc
     5641 atatgacaaa cgtgctgatg aagaagctgc aaaatatggt attcaactta cacatgtgaa
     5701 acattattat gaacaaccta aatttattga atattggacg aataaagtca acgaaacatt
     5761 agctcaaata ccggaagagg aacataaaga cacggtatta gttgtttcgg cacatagttt
     5821 gccaaaaggt ttaatcgaaa agaataatga tccatatcca caagaactag aacatactgc
     5881 gcttttaatt aaagaacaat ctaatattga acatatcgcg attggttggc aatctgaagg
     5941 taatacaggt acaccttggt tagggccaga tgtacaagat ttaacacgtg atttatatga
     6001 aaaacatcag tataaaaact ttatatatac gccagtaggt tttgtatgtg agcatttaga
     6061 ggtgctttat gacaatgatt atgaatgtaa agtagtttgc gatgatattg gtgcgaatta
     6121 ttatcgtcca aaaatgccga atacacatcc attatttatc ggtgcaattg ttgatgaaat
     6181 caagtctata ttttaatgac gaaagaagcg tgaaacgttg tgactaaatc agtggctatt
     6241 ataggagcgg ggataacagg tttatcaagt gcatattttt taaaacagca agatcctaat
     6301 attgatgtaa ccatctttga agcatcgaat cgtccggggg gaaagattca atcgtatcgt
     6361 aaagatggtt atatgattga actagggcct gaatcttatt taggtagaaa aacgattatg
     6421 acagaattag cgaaagatat tggattagaa caagatattg ttacaaatac gactggacaa
     6481 tcatatattt ttgcgaaaaa taaattatat ccgattccag gtggttcaat tatgggtatt
     6541 ccaacagata ttaaaccatt tgttactaca aaattaatat cgccacttgg taaattaaga
     6601 gcagggtttg atttattaaa aaaacctact caaatgcagg atggtgacat ttctgttggt
     6661 gcatttttca gagcaagatt aggtaatgag gtacttgaga atttaataga gcctttaatg
     6721 ggtggtattt atggtaccga tattgataaa ttaagtttga tgagtacgtt tcctaatttt
     6781 aaagaaaaag aagaggcatt cggaagtctg ataaaaggta tgaaggatga gaaaaataag
     6841 cgtctgaaac aaagacaatt atatcctggc gcaccgaaag gacaattcaa acaatttaag
     6901 catggtttaa gctcatttat tgaagcatta gaacaagatg ttaaaaataa aggtgtgaca
     6961 atacgctaca atacgtcagt ggatgatata attacatctc aaaaacaata taaaattgtt
     7021 tacaatgatc aactagaaga agtgtatgat ggcgtattag taacgacacc acatcaagtg
     7081 tttttaaatt ggttcggaca agatccagca tttgattact ttaaaacgat ggatagtacg
     7141 actgttgcaa ctgttgtatt ggcatttgat gaaaaagaca ttgaaaatac ccatgatggt
     7201 actggcttcg taattgcgag aacgagtgat acagacatta ccgcatgtac ttggacatcg
     7261 aaaaaatggc catttactac accggaaggt aaggttttga ttcgtgcgta tgtaggtaaa
     7321 ccaggtgata ctgtggttga tgatcataca gataatgaat tagtatcgat tgtacgtaga
     7381 gatttaagtc aaatgatgac atttaaaggt gatcctgaat ttacaattgt caatcgtttg
     7441 ccgaaaagta tgccacagta ccatgtcggt catattcaac aaattagaca gattcaagca
     7501 catattaaac aaacatatcc acgacttaga gtaactggtg catcttttga agcggttgga
     7561 ctacctgatt gtattacgca aggtaaagtt gctgctgaag aagtaatcgc agagttgtaa
     7621 gttaattgtt ggatggtgtt ggtgtaggtg cgacgccttt agataatgtt tgatgaaata
     7681 tagtcatttt taagttatat aatggtggaa gtaatgtgta aatatagata ctttcagcgg
     7741 gaattatttt tgtgaaattg aagcagagac aaaggtttag ctgtttttga atgatattga
     7801 aatgatgaca atacacttta attagaaatg tcactttgcg atataacatt tctaattaag
     7861 gtgtttttat tttacaatta taggtaatca gtcatttttt taaaaataga aaagaagtag
     7921 taagttgaaa ggagagaatg agatgacaga tgtaattatt gtacattcta tgcatggaaa
     7981 tagtagaaat cattggtatc agtggttgga gcataactta actttagaag gatatgacgt
     8041 aacattattt aattttgaat cccctgaagc gaagacagtg gatcagtgga ttgaagctat
     8101 gacgaaacaa attaatgtac gcaaaaagga tacatatttt gtaacacatg gtttaggttc
     8161 aatcacagct ttaaaatata ttgaaatgat tgatcagccg attgaaggct ttttcagtat
     8221 agcgggcttt aaagaagatg cagaaaatat agatttagat attgatttaa gtaatgtaac
     8281 cattgattac gataatatta aaaagaaagt tgacaatttt tacggattga gttctaaaaa
     8341 tgacaaatac gtttcatata aagagaccca gcgtttaatg aatgcattag aggggaactt
     8401 gcgaatcgtg gaagatggag ggcacttctt agaagaggat ggcttcgaaa catttacagc
     8461 gttgcaagat cggatgcaag attacatgac acgatagtta gtgtatgggt tgtgttatgt
     8521 tgtgtcagta ggtagctgct gattttgaaa aattgttgta attgaggcgt caagcaatgt
     8581 cttagggcta caatgttata ttggtagtcg ctgactgaaa gaatatgcgg tgtaacaagc
     8641 ttttataatt tctttttcag aaggtatagg cgttgtaaac tttataactc ctataccttt
     8701 ttaaatgctt taacactatt tatgacaact attagacact tatatgataa aagggaataa
     8761 gaagcgtttt tttacacaaa aaactttgaa aatgtcgttg acgtacacta caaaaatgaa
     8821 tatactaata tttgtgttca acagagaaca tgttgctacg atatttaagt aattatttaa
     8881 gagataacat ttaaattaaa ttcaaaaagt tcttgacttt aggtttttga ttatggtata
     8941 ttaattaggt aattcaaaat aatattagcg cgggat
//
LOCUS       DANRDI010000108         8943 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.108, whole genome shotgun sequence.
ACCESSION   DANRDI010000108 DANRDI010000000
VERSION     DANRDI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.108"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            117..209
                     /locus_tag="PZ377_001953"
     CDS             117..209
                     /locus_tag="PZ377_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358820.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            341..574
                     /locus_tag="PZ377_001954"
     CDS             341..574
                     /locus_tag="PZ377_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDM6358821.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            685..1950
                     /gene="murA"
                     /locus_tag="PZ377_001955"
     CDS             685..1950
                     /gene="murA"
                     /locus_tag="PZ377_001955"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDM6358822.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            1984..2424
                     /gene="fabZ"
                     /locus_tag="PZ377_001956"
     CDS             1984..2424
                     /gene="fabZ"
                     /locus_tag="PZ377_001956"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDM6358823.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(2483..2923)
                     /locus_tag="PZ377_001957"
     CDS             complement(2483..2923)
                     /locus_tag="PZ377_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDM6358824.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            3117..3464
                     /locus_tag="PZ377_001958"
     CDS             3117..3464
                     /locus_tag="PZ377_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDM6358825.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            3901..4596
                     /gene="sceD"
                     /locus_tag="PZ377_001959"
     CDS             3901..4596
                     /gene="sceD"
                     /locus_tag="PZ377_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDM6358826.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      4800..4902
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4982..5671
                     /gene="tenA"
                     /locus_tag="PZ377_001960"
     CDS             4982..5671
                     /gene="tenA"
                     /locus_tag="PZ377_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDM6358827.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            5664..6494
                     /gene="thiD"
                     /locus_tag="PZ377_001961"
     CDS             5664..6494
                     /gene="thiD"
                     /locus_tag="PZ377_001961"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDM6358828.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            6478..7269
                     /gene="thiM"
                     /locus_tag="PZ377_001962"
     CDS             6478..7269
                     /gene="thiM"
                     /locus_tag="PZ377_001962"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDM6358829.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            7271..7912
                     /locus_tag="PZ377_001963"
     CDS             7271..7912
                     /locus_tag="PZ377_001963"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDM6358830.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHISVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            7998..8870
                     /gene="yidC"
                     /locus_tag="PZ377_001964"
     CDS             7998..8870
                     /gene="yidC"
                     /locus_tag="PZ377_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDM6358831.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
BASE COUNT         3163 a         1333 c         1651 g         2796 t
ORIGIN      
        1 tatttcttta tgttggggcc ccgtcaacta ctgccaaata caacactata gagtctagac
       61 attgatttat gtccgactcc caagaatagt tttacttttt tacaatcact aatagattgc
      121 taaaatcaaa atttccttca ccactatcta cagtcgacat ttcatttttt gaaattatct
      181 acattttttc ataccaagat attttatagt tatgatattt atgtaaaaag aattatatag
      241 taagttagct taaactttac taaaaacggg tattaaactt tgtatcatta tttaaatttt
      301 tcatgtacaa tgtaatacag taatcttatg aggtgataaa atggattata tcggacaata
      361 tgcagttatc catttagtgt tacatgttgt atgtatttgt attgcctatt gggctttaca
      421 atcaattaga ttagatcaat tttttaaaaa aggatacgcc actcaattac aagtgtgtat
      481 gatatttgtt gctattttat taggcactgc agtaagcaat tttattgtag atttgttaca
      541 atactcgacg caggtaaaat atttaataaa ataagtctaa ctctatgatt tgtaatcaaa
      601 actagatata attaaataat gacttaaaat aattttaaaa tagggaaatg taaagtaata
      661 ggagttctaa gtggaggatt tacgatggat aaaatagtaa tcaaaggtgg aaataaatta
      721 acgggtgaag ttaaagtaga aggtgctaaa aatgcagtat taccaatatt gacagcatct
      781 ttattagctt ctgataaacc gagcaaatta gttaatgttc cagctttaag tgatgtagaa
      841 acaataaata atgtattaac aactttaaat gctgacgtta catacaaaaa ggacgaaaat
      901 gctgttgtcg ttgatgcaac aaagactcta aatgaagagg caccatatga atatgttagt
      961 aaaatgcgtg caagtatttt agttatggga cctcttttag caagactagg acatgctatt
     1021 gttgcattgc ctggtggttg tgcaattgga agtagaccga ttgagcaaca cattaaaggt
     1081 tttgaagctt taggcgcaga aattcatctt gaaaatggta atatttatgc taatgctaaa
     1141 gatggattaa aaggtacatc aattcattta gattttccaa gtgtaggagc aacacaaaat
     1201 attattatgg cagcatcatt agctaagggt aagactttaa ttgaaaatgc agctaaagaa
     1261 cctgaaattg tcgatttagc aaactacatt aatgaaatgg gtggtagaat tactggtgct
     1321 ggtacagaca caattacaat caatggtgta gaatcattac atggtgtaga acatgctatc
     1381 attccagata gaattgaagc aggcacatta ctaatcgctg gtgctataac gcgtggtgat
     1441 atttttgtac gtgatgcaat caaagaacat atggcgagtt tagtctataa actagaagaa
     1501 atgggcgttg aattggacta tcaagaagat ggtattcgtg tacgtgctga aggggaatta
     1561 caacctgtag acatcaaaac tctaccacat cctggattcc cgactgatat gcaatcacaa
     1621 atgatggcat tgttattaac ggcaaatggt cataaagtcg taaccgaaac tgtttttgaa
     1681 aaccgtttta tgcatgttgc agagttcaaa cgtatgaatg ctaatatcaa tgtagaaggt
     1741 cgtagtgcta aacttgaagg taaaagtcaa ttgcaaggtg cacaagttaa agcgactgat
     1801 ttaagagcag cagccgcctt aattttagct ggattagttg ctgatggtaa aacaagcgtt
     1861 actgaattaa cgcacctaga tagaggctat gttgacttac acggtaaatt gaagcaatta
     1921 ggtgcagaca ttgaacgtat taacgattaa ttcagtaaat taatataatg gaggatttca
     1981 accatggaaa caatttttga ttataaccaa attaaacaaa ttatacctca cagacagcca
     2041 tttttattaa ttgataaagt agttgaatat gaagaaggtc aacgttgtgt ggctattaaa
     2101 caagtatcag gaaacgaacc attctttcaa gggcattttc ctgagtatgc ggtaatgcca
     2161 ggcgtattaa ttactgaagc gttagctcaa acaggtgcgg tagctatttt aaatagtgaa
     2221 gaaaataaag gtaaaatcgc tttatttgct ggtattgata aatgtcgttt taaacgtcaa
     2281 gtagtacctg gtgatacttt aacgttggaa gtagaaatca ctaaaattaa aggaccaata
     2341 ggtaaaggta atgctaaagc tactgtcgat ggtcaacttg cttgtagttg tgaacttaca
     2401 tttgcaattc aagatgtaaa ataaaacaaa aaaaacattc aaagatttaa tgtgttggca
     2461 taatctttga atgtttttta ttttactctt ctaatttttc atcctttaac tttggtttag
     2521 actgcatcat tcgattaaat gattttttta attcttcacc agataatcca tcatcaataa
     2581 gttgttctaa taaactttca gcatactgtt gacgtaaagt gtaaatatga cattcaaata
     2641 cgatagacat tgttgtttta aaatttttaa tacttttcac agtagattca aataaagctg
     2701 gatcattggc tggacgcgtg atgacgacac gaatatccaa taactgttca ttgtcttgat
     2761 attccttaaa tgtttcataa tgctcattag aaatgacaat ttcaagcaac caacccgtgc
     2821 cactttcctc tttattaatt attacgccat cttctaattc atattctata attctgccgt
     2881 gttcattcac aatttgaaac cttactgctt taaaagtttt catattccca catcccatta
     2941 gtttaatatt tatgattttt gaattattta gaagatttaa ggtcatttca tgactaattt
     3001 ctttaaatat aacaaataac gagatgctat agctattaat tatataaaat ttgccatttt
     3061 gaccattttt aagctagtaa ttaagatata ggcataggag gtgaatcaca cactacatgc
     3121 taaataaaat cgtaattgtc gggagactga cgaaagacgc acaaatattt gaaaaggagg
     3181 atagaaaaat tgcaacgttt tgtgttgcaa cgcaccgaaa ttataaagat gaaaatggag
     3241 aaatcgtctg tgattactta ttctgtaaag catttggcaa gttagcttct aatatagaaa
     3301 aatatactaa tcaaggtaca ttggttggta taactggtca aatgagatca agaaagtatg
     3361 ataaagacgg acaaacacac tttgtcactg aattatatgt tgaaacaata aaatttatgt
     3421 cccctaaatc ccaaaataat gaaattctct cagatagtat ttagatattg actctcaaaa
     3481 tatagataat catgacttat tagaaattta aatccaatta tcctaaacat ccttaatata
     3541 catttaatcc atcctaaatt ttttatagat ctaattaact tgaaatatac tcacttaaaa
     3601 acgacttaca tgactagtac tgttgtgtag gtcgtctttt tgtgtagttt ttctaataga
     3661 aaatagacat agattgcaaa acgattacat aaaaaattgc aaataaaaaa acatttttac
     3721 tgtcatgaca attctcgata gaatcatatc tgaaattatg ggggatttcg ttgtaatcac
     3781 tgtgtaaaaa acaaggctat ttttttattt tgttgtaacc tgcacggatt ttatagatgt
     3841 tacagtagtc tctgtaaaac ttttagagaa acaaattact tgtaggagga ttttaagatt
     3901 atgaagaaaa cattactcgc atcatcatta gcagtaggtt taggaatcgt agcaggaaat
     3961 gcaggtcacg aagcccatgc aagtgaagcg gacttaaata aagcatcttt agcgcaaatg
     4021 gcgcaatcaa atgatcaaac attaaatcaa aaaccaattg aagctggggc ttataattat
     4081 acatttgact atgaagggtt tacttatcac tttgaatcag atggtacaca ctttgcttgg
     4141 aattaccatg caacaggtac taatggagca gacatgagtg cacaagcacc tgcaactaat
     4201 aatgttgcac catcagctgt tcaagctaat caagtacaat cacaagaagt tgaagcacca
     4261 caaaatgctc aaactcaaca accacaagca tcaacatcaa acaattcaca agttactgca
     4321 acaccaactg aatcaaaatc atcagaaggt tcatcagtaa atgtgaatgc tcatctaaaa
     4381 caaattgctc aacgtgaatc aggtggcaat attcatgctg taaatccaac atcaggtgca
     4441 gctggtaagt atcaattctt acaatcaact tgggattcag tagcacctgc taaatataaa
     4501 ggtgtatcac cagcaaatgc tcctgaaagt gttcaagatg ccgcagcagt aaaattatat
     4561 aacactggtg gcgctggaca ttgggttact gcataagcca tttatgcata gctaattaat
     4621 agttatataa gtaactttta gattggaata tatcggggaa ttctcgatat gttccggttt
     4681 ttttatttcg caaatatatg tcattataga attattattt aaagataata atacaactga
     4741 gcaacaataa ttgaaaatgt tgaaaccgtt tgctaagatt aatttgcaat tgaatactaa
     4801 ggaactacta ggggagccta atgatatggc tgagatgaat tgttcagacc cttatgacct
     4861 gatttggtta gtaccaacgt aggaaagtag ttattgaagt gatggggcta gtatagcaac
     4921 attgccatca aactactttc atgttggaag tagttttttt atttagggag gattttttac
     4981 aatggaattt tcacaaaaat tgtaccaagc tgcaaagcca atcattaatg atatttatga
     5041 ggatgacttt attcagaaga tgttatccgg agatatagga gctgacgcat tacgccatta
     5101 cttaaaagct gatgctgcgt atttaaaaga atttacaaac ttatatgctt tgttaattcc
     5161 aaaaatgaat agcatgaatg acgtaaaatt tttagtagag caaatagaat ttatggttga
     5221 aggtgaagta cttgcgcatg atattttagc gcaaattgtt ggtgaatcat acgaagaaat
     5281 cattaaaaca aaagtttggc ctccaagtgg cgaccattac ataaaacata tgtatttcca
     5341 agcgcatagt cgtgaaaatg ctatttatac aattgctgca atggcacctt gtccatatat
     5401 ttatgcagaa ttagctaagc gctctcaaag tgatcataag cttaatagag aaaaagatac
     5461 ggcaaaatgg tttgattttt atagtactga aatggatgac ataattaacg tatttgaggc
     5521 attaatgaat aaattagccg aatcaatgtc tgacaaggaa ttggaacaag taaagcaagt
     5581 atttttagaa agttgcatac atgagcgtcg atttttcaat atggctatga cactagaaca
     5641 atgggaattt ggaggaaaag taaatgatta aacctaaaat agcattaacc attgcaggta
     5701 ctgatccaac aggtggtgcc ggcgtaatgg ctgatttaaa atcatttcat tcatgtggtg
     5761 tatatggtat gggcgtcgtt acaagtattg ttgctcaaaa tacattgggc gtacaacata
     5821 ttcataattt aaatcatcaa tgggtagatg aacaacttga tagtgtcttc aatgatacct
     5881 tacctcatgc tattaaaacg gggatgattg ctacagcaga tactatggaa acgattcgtc
     5941 attatttaat gcaacatgaa tctattccat atgtaattga tcctgttatg ttggcgaaaa
     6001 gcggtgattc attaatggat aatgacacaa agcaaaactt gcagcatacg ttattgccat
     6061 tagctgacgt agtaacaccg aatttaccag aagctgaaga aataacggga ctaaccattg
     6121 atagtgaaga aaaaattatg caggctggcc gcatctttat taatgagatt ggtagtaaag
     6181 gtgtcatcat taaaggcggt cattcaaatg atactgatat agcaaaagat tatttattta
     6241 ctaacgaagg tgttcaaaca tttgaaaatg aacgatttaa aacaaaacat acgcatggaa
     6301 cagggtgtac attttcagca gttataacgg cagaacttgc aaaaggtaga ccattatttg
     6361 aggctgtaca caaggctaaa aagtttattt caatgagtat acaatatacg cctgaaatcg
     6421 gccgtggtag aggtccagtg aatcattttg catatttaaa gaaagaggga ttagacgatg
     6481 aattatctaa ataaaatacg tattgaaaac ccattaacaa tttgttatac aaacgatgta
     6541 gttaaaaatt ttacagcgaa tggtttatta agtattggtg ctagccctgc aatgagtgaa
     6601 gctcccgaag aagctgaaga attttacaaa gttgcacaag cgctattaat caatatcggt
     6661 actttaacag cagaaaatga acaagatatt attgcgattg ctcaaacggc aaatgaggca
     6721 ggcttaccta ttgtatttga ccctgtagct gttggtgctt ctacatatcg aaagcaattt
     6781 tgtaaattat tattgaaatc agcgaaagta tcagtaatta aaggcaatgc atctgaaata
     6841 ttagcgttga ttgatgatac agcaactatg aaaggtacag atagtgatgc taatcttgat
     6901 gcggttgcaa tagcgaaaaa ggcttacgca acatataaaa ctgcaatagt aatcacaggt
     6961 aaagaggacg ttattgttca agataataaa gccttcgtat tagctaatgg atctccatta
     7021 ttagcacgag taactggagc tggttgttta ttaggaggcg ttattgctgg atttttattt
     7081 agagaaacag aaccagacat agaagcgtta attgaagcgg taagcgtatt taatattgct
     7141 gctgaggtag ctgctgaaaa tgaaaattgt ggtggtcctg gtacgttttc accattgttg
     7201 cttgatacgt tatatcattt aaatgaaaca acctatcaac aacggattcg tattcaagag
     7261 gtggaataat atgtttaacc aatcgtatct aaatgtgtat tttatttgtg ggacctccga
     7321 tgttccgagt catcgaacta ttcatgaagt attagaagca gcattaaagg cagggattac
     7381 acttttccaa tttcgagaaa agggtgaatc agctttaaaa ggaaacgata aacttgtatt
     7441 agctaaagaa ttacagcatc tctgtcatca atatgatgtg ccttttatcg ttaatgatga
     7501 tgtatccttg gcaaaagaaa ttaacgcgga tggtatacat gtcggtcaag atgatgcgaa
     7561 agtcaaagag atagcacaat atttcactga taaaattatt ggacttagta ttagtgattt
     7621 agatgagtat gcaaaatctg atttaactca tgttgattat ataggtgttg gaccaatcta
     7681 tcctacgccg tcgaagcatg acgcgcatat atcagtaggt ccagaaatga ttgcaacgtt
     7741 taaagaaatg aatccgcaac taccgattgt agctattggc ggtatcaata ctaataatgt
     7801 ggcgccgatt gtagaagcag gtgccaatgg aatttcagtc atttcagcca tttctaaaag
     7861 tgaaaatatt gaaaagactg ttaatcgatt caaagatttt tttaataatt agttaaattt
     7921 ttccacttat tgtttcatac gtactcatta atgtgataga ataaatccta tgtgaatata
     7981 atgatagagg tggaaaaatg aagaaaaaag cgttactacc attattttta ggtattatgg
     8041 tctttttggc tggttgtgac tattctaaac ctgaaaaacg tagtgggttt ttctacaata
     8101 cattcgtaga tccaatgaaa aatgtattgg attggttggg aaataactta ttaaacgaca
     8161 attatggttt agctattatt atccttgtat tggtaattcg tattatttta ttaccattca
     8221 tgttgtcaaa ctataaaaat agtcatatga tgcgtcaaaa aatgaaagtt gcaaagccag
     8281 aagttgaaaa aattcaagaa aaagtgaaac gtgcgcgtac acaagaagaa aaaatggctg
     8341 caaaccaaga attaatgcaa gtatataaaa agtatgacat gaacccgatt aagagtatgt
     8401 tgggttgttt accaatgcta atccaattac caatcatcat gggattatac tttgtactta
     8461 aagaccaact tgtagatggt ttgtttaaat atccacactt cttatggttc gatttaggac
     8521 gtcctgatat ttggattaca attattgccg gtgttttata ctttatccaa gcatatgtat
     8581 caagtaaaac gatgccagac gaacaacgtc aaatgggtta catgatgatg gtcatttcac
     8641 caattatgat tatctggatt tcattaagct cagcatcagc acttggtttg tactggtcag
     8701 tcagtgcggc gttccttgta gttcaaacac actttgcgaa catttattat gaaaaagtcg
     8761 ctaaaaaaga agtacaacct ttcattgaag cgtatgaaag agagcacaac ggcggcagca
     8821 ataaaaaagg caaaaacaca caagttgtgt ctaaaaagaa aaagaaataa ccttagcaag
     8881 tccaaataat gtgcacccta aaagttgaag ataattttaa ctttataggg cgcacattat
     8941 ttt
//
LOCUS       DANRDI010000109         8869 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.109, whole genome shotgun sequence.
ACCESSION   DANRDI010000109 DANRDI010000000
VERSION     DANRDI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.109"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1268
                     /locus_tag="PZ377_001965"
     CDS             <1..1268
                     /locus_tag="PZ377_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6358832.1"
                     /translation="TFRVATGDIQPHMHASVGTAVPGVTLFLLLKAFSSGASSLTGVE
                     AISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGILPQTETTVLSQLAM
                     QIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYMPRMFTVRGDRLGYS
                     NSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMVIKWIHERPKNWLSK
                     LSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKINKHYRDIAEQLRSDI
                     DVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHVSFGDEDEKAFQEKW
                     KRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVVIPEFITKKRWHNLL
                     HNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(1408..3486)
                     /locus_tag="PZ377_001966"
     CDS             complement(1408..3486)
                     /locus_tag="PZ377_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDM6358833.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(3624..5033)
                     /locus_tag="PZ377_001967"
     CDS             complement(3624..5033)
                     /locus_tag="PZ377_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6358834.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            5320..6126
                     /locus_tag="PZ377_001968"
     CDS             5320..6126
                     /locus_tag="PZ377_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDM6358835.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(6189..6845)
                     /locus_tag="PZ377_001969"
     CDS             complement(6189..6845)
                     /locus_tag="PZ377_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDM6358836.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            6958..7431
                     /locus_tag="PZ377_001970"
     CDS             6958..7431
                     /locus_tag="PZ377_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDM6358837.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(7554..8765)
                     /locus_tag="PZ377_001971"
     CDS             complement(7554..8765)
                     /locus_tag="PZ377_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDM6358838.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
BASE COUNT         3069 a         1352 c         1607 g         2841 t
ORIGIN      
        1 gtactttccg tgtggcgaca ggagatattc aaccacatat gcatgcatca gtaggaactg
       61 cggttcctgg agtaacatta ttcttgctat taaaagcatt ttcatcaggg gcttcatcgt
      121 taacgggtgt tgaagctatt tcaaatgcgg taaccaattt tagagagcca agtgctaaaa
      181 atgctgtgaa aacgttaatt gctatgggtt caattttagc gtttttatta gttggtatcg
      241 ttggtttagc atatgtttat ggaattttgc cacaaacgga aacgacagtt ttatcacaat
      301 tagctatgca aatatttggt gataatgctg ccttctattt tgtacaggca acgaccgtga
      361 tgattttggt attagcagcc aacactggat ttacagcttt tccgatgtta gcggcttcaa
      421 tgtctaaaga taaatatatg cctagaatgt ttactgttag aggtgatcgc ttaggctact
      481 ctaactcgat tattatttta ggtgtattag ccattatttt aattattgta tttgatggta
      541 tgactgaaga tttaattcca ttgtatgcgg ttggtgtatt tattccattt acactagcac
      601 aattcggtat ggttattaaa tggattcatg aacgtcctaa gaattggtta agtaagttgt
      661 ctgtgaactt acttggtggt atcgtgacat ttattgtttt tatgatttta ctcataacta
      721 aatttagcca agtatggccg atacttattt tcttgccgtt cgtggtgatt ttcttcttga
      781 aaattaacaa acattatcga gatatcgcag aacaattacg ttctgacatt gatgttctta
      841 atgtggacgt ggtagatcgt aatttagcaa ttgtgccgat aacaagtatt acaacagcag
      901 ttgataagtc tatttattac gcacaaatgc ttgcgaataa tgatgtcata gcagtacatg
      961 tatcatttgg agatgaagat gaaaaagcat tccaagagaa atggaaacgt catttcccag
     1021 atgtaagatt agttatttta cattcggaat atcgcagtat tatacgacca atttcacgtt
     1081 ttatcgataa gattaatcgt aaagccaacg atcaaaacta tatgattaca gtagtgatac
     1141 cagaatttat tactaagaaa cgttggcata atttattaca caatcaaact agtttgcgta
     1201 tgaaattgta tttaatttat caaaagaatg ttaacgtatg tacaattcca tttaaactta
     1261 aaaaatagat tctataatca aatcggattg atgaaaggtt atttactttt catcaatctg
     1321 attttttgat gttgaatata aaaaccccgt acatatgaga tgataatgac tttgtctcat
     1381 atgtacgggg gtattgttat gtcttgctta atgtttggac tgtaaagaca agacaatttc
     1441 gttgtaattg atattttctc gaatttgagc tgcaacgata ttgttgattt tatttttgcg
     1501 aattaattca tctaaaagat gacgttgtaa gtacaatgct tccaatttgt ggttatggaa
     1561 catagcttgc ggtatgattt gattgctgtt ttcttgacgt tcttttcgac gttgtgcacg
     1621 ccactttaat gagcgactca tatcatagta atgattaatg actaagctaa cctcaagtac
     1681 atttgtacta ttttgttctt cttttaagcg taaaataata ttgtgattca caatacgcat
     1741 aattttttga acatctttga atgaattttc aaattcttgc ttcttagatt ctttatttaa
     1801 ggctttgtag tgcttcatcg ctttaagtct aagccataac atcttgaaga agtttgcaaa
     1861 tttttcaaca aatgatgcag tacggtgtgt ctctgttaat tcgacaatat tgcggtaatt
     1921 attaatatcc tgatatgttg ccttaccttt atcaattaaa cgctcaagtg tagatgtttc
     1981 gattactttt gcaatatctt ctaaacgttt gagttctttt gtattttgat tatcaggttc
     2041 tgaatttaat aaaaataaca attctccata gtattggttt aatactgggc gataatttgt
     2101 atcgtttttg tcttttttac tttcgttttt aaaatgctgg atcacttttt gaacaatgaa
     2161 aattttggca gattgataag tcataccttt aaaagtagta tcttcggcag atggtgtaat
     2221 taaaggtaaa acaatttgcg ctaagattaa actaattaat accatgaaag atgcaataaa
     2281 caataaatca ttacggtatt cgaatgcttg tccttttgta atgataaatg gtaatgtaag
     2341 tgccattgaa agtgaaattg taccgtgaat accacacatg gtcataataa atgcgtaacg
     2401 cactcgagaa ggtggtgttt catgtgaatc atgttcctcg tctagataag attgtatatt
     2461 tttcgggaaa taaaaatctt tgtaccagaa ataaacccaa acaaatcgac atgcataaat
     2521 tgcaatagcg attaatatag tgatcactat taaaaatgaa aagttgtcag gctctgtttg
     2581 gaaaatatct atgactacag taggaatcat aaaacctaat acaacaaata caaaaccatt
     2641 taaggcataa ctaaatgtgt tccaaatttg atggtaattc atttgtagtt ccgtttgtgc
     2701 tctaattaaa cggtcgcgtt ctaaaccatg gattagacct gcgattacaa ctgcaatgat
     2761 acctgaagca tgaacttctt ctgctaaaaa gtatacgaca aaaggagtta ataattgaat
     2821 aaaagttaag gtattgttat cttttaaacc tttattagcg gttaagtcta tacgtattct
     2881 aacgacaacg aatccaataa ttgcaccaat aagtacacct agtattgttg aaatgataaa
     2941 ttgttcaaca gcttggaata atgaaaaggt accagttact aatgcagtaa cagcaatttt
     3001 aaatgaaatg atacctgctg catcattgag taaagattca ccttctaaaa tcgtcataga
     3061 acctttaggt aataattttc cgcgtgtaat agcagatact gctactgcat cagtaggaca
     3121 taaaattgct gctattgcaa aagcggctgg cattggtaag gcaggccaaa tccaatgtat
     3181 aaaatagcca acaccgacta cagttgcaaa cactagtgcc attgacatta atagtatagg
     3241 tttacgatat tctaataatt ttgttcgaga gacgtgggta ccttccacaa aaagtagtgg
     3301 cgcgataacg gcaaacataa atacttcaga attgaattgg aaatcaactt gtattggaat
     3361 aatgaaaata acgacaccta atgcaatttg aataaaggca gtaggaattt gtgggaatcg
     3421 attattgata accgaactaa taatcacagc aaaaataaaa attaaaaatg cttctaatag
     3481 tgccatacaa tactcctcaa aattttaata gttaatattt tatcactttt aaggcataat
     3541 gacatagata tattgataaa atgaagttat tttcaaaaaa actctagtat cggttgaact
     3601 gatactagag cgagatgttt aaattattga ttgtcatatc tgaaatgacc gctgtcattt
     3661 tgtcgttgtt catacgcgag cttttcagca ttcgttttgt attttttata aaagaaaaat
     3721 aaaaatataa accagaatgg cgaaatataa atagctgctc ttgtttcgtc actaaagaat
     3781 aataaaatga atacaaagaa gaagaacgct agaataatgt aagcaatagg cttaccacca
     3841 atcaacttaa atttactgtt tttatgtgcc tcaggatgct ttttcaaata cattagataa
     3901 gcgacaataa tcattgccca aacaactaaa aacaacacag ttgataacgt tgtaacgtat
     3961 atgaatagtt gaattgcatt agggaaaatg tagtttaaca aggctgctat aagtaatgca
     4021 attgatgaaa ctaacattga taaataaggc acgccatgac tattcgtttt atttaaaact
     4081 ttaggaccta acccttgttg tgacagtccg aataagatac gactattcga atagatacca
     4141 ctatttgtag cagaggccgc ggcagttagc acgacaaagt taacgacacc tgctgcaaat
     4201 ggtacgccga ttaatgtaaa gagtttaacg aaagggctac tatttggatc gatatcattc
     4261 caaggtatga ctgacataat tactaataga ccaccgatat agaataataa aatacggata
     4321 ggtacattat taattgcttt cggtaaggtt ttttcaggat ctttcgtttc accagcagtt
     4381 acacctataa gttcaatacc aatgaatgaa tatacagcaa tttggaatga cattaagaaa
     4441 ccaaatgttc caccagggaa catgccaccg tgactgatta agtttgtgaa tgatgcatgt
     4501 ccataatgtg ttttaaatga gaaaaagatt aatacaagac caacgataac catcgcaata
     4561 attgtgacaa ctttaataat cgagaaccaa aactccagtt caccaaataa tctggcacct
     4621 aataagttga agctaatcaa gattaaaaca ataaatagca cggtaatcca atttgggact
     4681 tgtggatacc aaaatgcaaa gtattgtccc atcgcagtta ggtctgacat acttgataca
     4741 atccaacata accagtaagt ccagccaatg acaaagccac caaaagggcc taaatattca
     4801 tttgcaatat caacaaatga attaaatctt gtattgctca acaacaattc gcctaatgcg
     4861 cgcataaaag cgaatagtac aaccccaata atcatgtatg tgaataacag tgatggacca
     4921 gttaaagata ttgtttgacc tgatcctagg aataacccag ttccaattgc gcccccaatt
     4981 gctattaatt ggatgtgtct gttattcaac tctctatgca attttcttgc cataattttc
     5041 ctcctcagga taaagcaaat aaaaaagttg tgtgtaagcg ttttcttaat tataaatact
     5101 aaagaaatcc atttaataaa gcaattattt ttttaaatat aagaaaaaac atttgatagg
     5161 taagggtatg ataactaaaa cttatcgatt ttttgaaaaa gatataagtt tttatgcaaa
     5221 aatataaaat aatcatctaa atgaagcagt taactatata tattagataa gctatggcat
     5281 aataaataac attttaattt gagtaaagaa ggtaagggta tgaagatagg gacaatcgcc
     5341 gatttacata ttgatcgtca taataaaaaa acatccgaag attacttaga ggcattggtt
     5401 gaaatagtta agtataagaa attggatata ttgttgattg cgggggatat ctcaaatcat
     5461 tatcaattga cgcaccaatt tataacgcaa ttgacaaaac aattagatat acctgtgaaa
     5521 tttgtacctg gaaaccatga tttatgggaa gttgaaagta tgactacgca agacatttgg
     5581 aataattata agagtatgtc acagtgcttg gtaggaaaac catttatagt aaatgaagaa
     5641 tgggcaatca taggacatac tggctggtat gattatagct ttgcagcaca acgattttca
     5701 ttagatgagt tacaaaaagg aaaacattat ggtgcgactt ggcaagataa agaacgaata
     5761 tcttggggca tatcagatca aaatttatct aaaatagcgg ctgaacaagt gaagaaagat
     5821 atattagaag taggaaatag acgagtgatt ttagtcacac atgttgtgac gcaccctgat
     5881 ttcattgttc ctatgccgca tcgtatattc gatttttata atgcatttat tgggacaagt
     5941 gatttcaatc ctttgtatgc gatgttcgat ataccatata gtattatggg ccatgttcat
     6001 tttcgtaaaa gtgtgataga tgatggcaga tgttatctct gtccgtgtct aggctatcca
     6061 agacaatggc gttcagaaga tatttaccag gaaataaatg agacgataca aataatagaa
     6121 atttaaaatg cgcaaacctg acccagtttg cgcattttat gttttacaca cgcgagtaat
     6181 gtgtttactt acgtgtgttt attttgttgc tgattttcaa ttgtatatga atgtggttgc
     6241 acataaatgc actttcttcc tggtgaatta aagctgtatt ccattttctc tttacgaatt
     6301 ttaataattt gtttgcgatt tggaatgatg gcaggtaaaa ctaggccacg acgaatatga
     6361 ctccaaagtg gatcgatata atagtaatag taatcatcgt acccaatgag taacgttacg
     6421 tgaatatttg aaacttgttt cgctggcttg ttatcaaagc ggaaaacacg tagtaatggt
     6481 ttagaaccaa gattagtatg gtatattaac acaggttgac cttgatcgat aataccttta
     6541 agatcttcta acgatttacc agtgccgtct acgatattag gattgtattt ttgtaaaaat
     6601 ggtacatatg cttctggaaa tatcgtttga tgataattgc caagcttaat gaataagtga
     6661 tgtccaacat aacctttatg tggattgttc ggatgtgtcg gccaatgtct cataatttct
     6721 gtagcaggga tatgttggtt gttgtattgc aacatcatgg ctgcggaaac accttcacac
     6781 cccatgacca tagggatagg aaatagctga ctgataggtt taactggtaa tatttttcgg
     6841 ttcataatat agtcctcgca ttgattcaat aaatatttaa tataattata tagcgtcaat
     6901 gcaaaatgtc ctaaacatat gttttacatg agtgaataaa attaatggag tgataaaatg
     6961 gaatatcaat tacaacaact tgcgtcgtta acgttagtag gtattaaaga aacgtatgaa
     7021 aatggacgac aggctcagca acatatagca gggttttggc aaagatgtta tcaagaggga
     7081 gtaattgcgg atttacagtt aaaaaataat ggtgatttag ccgggatact tggcttatgt
     7141 atacctgaat tagacggtaa gatgtcatat atgattgcag ttaccggaga taatagtgct
     7201 gatattgaaa aatatgatgt cataacatta gcaagttcaa agtatatggt atttgaagca
     7261 cagggcgcag tacctaaagc agttcaacaa aaaatggaag aggttcatca ctacatacat
     7321 caatatcaag cagatacggt aaaatcagca ccattttttg agttgtatca ggatggtgat
     7381 actacaagtg aaaaatatat tacagaaatt tggatgcctg ttaaagggtg attgaataga
     7441 aatgtccttg ctatgaaggc gtaattttag ttgaagattc gatctcataa ttcataaaga
     7501 agaaccctga caaagataga tatctttgct agggttctaa ttatgttgaa gtgttatgcg
     7561 acatgttgct ttttaatcat tttaaagtaa atgatttgta gactgactaa tagaatggct
     7621 gtaatgaaga taataatcat atatggtgat gtattaaatt cgccttttaa gccaactaat
     7681 ggtgcaacag cgccacctaa gataaattgg aataagccaa gtaaacttga tgcgttacca
     7741 ctgccacctg ttcgttcttc catagccatt gtgaaaccaa gcggtccaat tgacgtcaca
     7801 ggacacacat ttaagaagaa tgcgatgagt aagacccaaa gtggtaaatg gaatgtaagt
     7861 gtcaggataa ttaaagcaac acctaccact tgtataatag ttaaaatgat taataatatg
     7921 tggcgatgta atttttctac taataaagca acgacttgac tgacaatgat taaacctaca
     7981 ccgttaacag caaacatgat actaaattgt tggggtgtca tattatacaa tttttgagta
     8041 ataaatggcg atgcagatga ataactaaat agcattacat aagttaaacc ttgtaatagc
     8101 attggaataa taaatgctgg ttttttcaaa agacttccaa aatctttaat gacgctacta
     8161 aaattcacct gctttaattc atctttagat gttttaggta attgagaagc gacgccaatt
     8221 aaaatgatga gtgccacaat agttaaaatt gtgaaaatag aacgccatgt tgctacggat
     8281 aaagctaatc caccggctaa tggtgcaaga atagtgatga tgccattaac taccattaaa
     8341 gatgctaaaa atttagcgag tgcgttgcca ctaaatttat caccagcaga ggcttttgca
     8401 atcacgatga cgccaccacc agttaaacct tgaataaatc gtaaagttaa gaataatggc
     8461 aattgatcaa caaacataga caaacctgat accaaaatga aaatgatcaa aatggataca
     8521 gcaatccgtt tgcgaccaaa agcatcggat aaaggtccaa agataaattg gcctagtgca
     8581 agaccaatca ttgtgaatga taatgttaat tgaatttctg atgtagtaga accaaaatca
     8641 ccttgcactt taggtaatga tggtacgtac atatctatag ttagtgggcc aaaagcggtc
     8701 atgacgccta acgtaattag caataaaaat gaaaaatgct tcgttttggt tttcgttgat
     8761 tgcatgatca gactccttta aatgatataa ttatataaat tatatacgtt ttgtgtgtat
     8821 ttgtatgtac ttagaataca agtttatata aaaaatccct atactctta
//
LOCUS       DANRDI010000110         8691 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.110, whole genome shotgun sequence.
ACCESSION   DANRDI010000110 DANRDI010000000
VERSION     DANRDI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.110"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            64..1554
                     /locus_tag="PZ377_001972"
     CDS             64..1554
                     /locus_tag="PZ377_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDM6358839.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
     gene            complement(1927..3245)
                     /gene="cstB"
                     /locus_tag="PZ377_001973"
                     /pseudo
     CDS             complement(1927..3245)
                     /gene="cstB"
                     /locus_tag="PZ377_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            3301..3438
                     /locus_tag="PZ377_001974"
     CDS             3301..3438
                     /locus_tag="PZ377_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358840.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            3435..3806
                     /locus_tag="PZ377_001975"
     CDS             3435..3806
                     /locus_tag="PZ377_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDM6358841.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            3935..4555
                     /locus_tag="PZ377_001976"
     CDS             3935..4555
                     /locus_tag="PZ377_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358842.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
     gene            complement(5075..5263)
                     /locus_tag="PZ377_001977"
                     /pseudo
     CDS             complement(5075..5263)
                     /locus_tag="PZ377_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5472..5642)
                     /locus_tag="PZ377_001978"
     CDS             complement(5472..5642)
                     /locus_tag="PZ377_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358843.1"
                     /translation="MPSRWVKVKTFKSLSTSKLEKKLQNFKSYNSFDIIEIKRHSYLF
                     LNIVEVYYKDKT"
     gene            6044..7009
                     /locus_tag="PZ377_001979"
     CDS             6044..7009
                     /locus_tag="PZ377_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDM6358844.1"
                     /translation="MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEK
                     EFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKP
                     LLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIE
                     SNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRC
                     AITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVL
                     SKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
     gene            7389..7718
                     /locus_tag="PZ377_001980"
     CDS             7389..7718
                     /locus_tag="PZ377_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358845.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
     gene            complement(7795..8154)
                     /locus_tag="PZ377_001981"
     CDS             complement(7795..8154)
                     /locus_tag="PZ377_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358846.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIFFLIAL
                     ILYILNINRGIKILLKK"
BASE COUNT         3319 a         1010 c         1398 g         2964 t
ORIGIN      
        1 tatggtagac ggtttgagta aattaatgga gtattttaat atttatggtt tggaggaatt
       61 gcgttgcata gagaaagtaa atatatagag tataagaaat cacgaaaagg attatctaat
      121 gatatttggt ctacgtatag tgcttttgca aatactgaag gtggtactat atatttagga
      181 attgaagaaa aaaagatcga ggacaaaaaa gtctttgttt cagttggtgt tgaagatcca
      241 gagaaaatga ttgaagattt ttggaatgca ctatatggaa gaagtaaagt tagtcaaaat
      301 attttatcaa ataaagatgt taaaattgtt aatattgaaa ataaagcgtg cattgaaatt
      361 catgtaccag aagcgcctta ttcgaagaaa ccgatatatg tagataataa aaaagattta
      421 gtatataaaa gagttgatga tgctgataga attgcgactg aagaagagta taaattcatg
      481 attgtaaatt ctcaagacga tatagataca gaattattag ataactatga catgtctgat
      541 ttaaatcacg aatctatcga aaattatagg aaacttctat taaaaaatac taatgatgag
      601 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
      661 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      721 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      781 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      841 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
      901 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
      961 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
     1021 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
     1081 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
     1141 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
     1201 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
     1261 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
     1321 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
     1381 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
     1441 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
     1501 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
     1561 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1621 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1681 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgtttt
     1741 ttgatcacat caataaacaa aaaaggtatg tactatgtaa aatatttatt aaatgatata
     1801 aagcgagggt atataaatga tttttaaata gatatttatc caataatata aaaaggaact
     1861 ataagctata tctaaaagct atatctaact acttatagtc cttcttcatt agtataaata
     1921 taattattaa gaaaggtata tatctttgta accttcgttt acattaataa tgttgtgata
     1981 acctttgtgt tctaaaatac caatagctat agaacttcta atgccagact gacagtctac
     2041 ataaataaca tcgtttctat tgaaaggtaa atctgtttct aaaagtttgc cgtgtggcac
     2101 atgaaccgct tgggataagt ggccattatt ccattcatta tcattacgta catctaagac
     2161 atgtgcttca ttaccagtta tgtctttact atgaacagat tgtgtttgaa tttgagcttg
     2221 tggtaactga tatccagaca cattatcata tccaataagt tgtaaagtat gttatgtgtt
     2281 gcttttgaaa caaggtgata gtctccaatc aagttaattt cttgattata gtttagatac
     2341 cagccaattt gattgatgaa atttttatca tatggaatat tgattgtacc ttcaatatgt
     2401 ccaccatgat aagcctcctt actgcggaga tcaaaagtta atctgtttgt acttgtagct
     2461 ggataaaccg tataaggttg atataaattc ataccgaatt gattaatttt tttcatttgt
     2521 gcaaaatgat gtggtggtgc aggttggtca gaaatgagtt tatcgataaa ggtagcttca
     2581 ttattttcag aaaaagccca gttcgtttgt ttttcatagc caagcgtaga tgttggaata
     2641 gcacctaaag atttaccaca aggactacca gcgccatgac caggccaaat ttgaatgtag
     2701 tctggcaagt ctttaatact ttcaatagat ttaaacattt gttttgcgcc tatttcagat
     2761 aatccttcta ctttaacagc tttttctagt aaatcaggtc taccgatatc tcctacaaaa
     2821 ataaaatcac cactgaatag tcccattgga acttgtgctc cagcaccttc gtcagtaagt
     2881 aaaaaactta tactttctgg cgtgtgacca ggtgtatgaa gcacttttaa ttttatattt
     2941 cctacataaa tatcatcatt atgttgaaca aaatgagtgt ggttaggcat atttttataa
     3001 cctaacatgt catcactttc gcccgataca taaatactag catttaactt tatagcaaca
     3061 tctctaattc ctgaaacaaa atttgcatgt atatgtgttt cagctgcatg agtaatggtt
     3121 aaactctctt catcggcaac tcgaatatat gaagataagt cacaaatagg atcaatgatc
     3181 atggcttctc cagttttttg acaaccgatt aaataagatg cttgagataa atgtttatca
     3241 taaattgatg atagtcaatt tgttatcaag ggtgataatt atattaacta tggtaattac
     3301 atggaattaa agttattaaa caaacttgat gatgaccaat tagatttaga atttttacaa
     3361 gataatgggt taaaaggtaa tgttcaaggt cctatgactt taaaggaacc tttaaaaagt
     3421 tatatacaaa agtcttgaaa acgaatattt taaatttaga taatgagtta gaaaacactg
     3481 aatatgtcga aggtaaacca tatgtacagt acggtattaa atatgaaaga aatcaggcat
     3541 taagaaatga agctattaaa attcatggaa ctacatgtaa agtatgtgga tttgatttta
     3601 aagctaagta tggcgattta ggtgagggtt ttattgaaat tcatcattta aaaccaatgt
     3661 tttcaataaa aagagaaata aaagtaaatc cacaaaaaga tttagtccca ctatgttcta
     3721 attgccataa aatgattcat agaaatacta aaaaaccttt aacgattaaa gaattaacca
     3781 aaatagttaa ttataatagc aaataattta atattttata aactatcatt caaccctctt
     3841 aatttattag gaggtttttt gtatttatgc tttcaaatgt gtgatatact ttgtttgttg
     3901 aaatatagag tatctataga tagggtgatt gagtatgaaa ttcactgaag tggaagttat
     3961 cgaacatctt gtaaaggcat ataaagaagc aggaaagcct acttatcctc atgaaaattt
     4021 atatcgagga cgtaatcata gtatttcagg tattggagaa gacttgctgg gtgcttattt
     4081 gattagtaga ttggaaggtg tccaaatatt tattgatcag cctttatcta tgattgataa
     4141 atctttaagt acaagatatc cggatttatt aatttgtgaa gataatgaaa ttaaaaatat
     4201 actagaagtt aaaatggact taggatatca aagaaaagat tttatagatt attgccgaaa
     4261 gaaagaagaa tggatttcaa atatcgtagg aaaacagtgt gtattgtcta gaaagagaga
     4321 agacaaaatt cctatgaata tagctgatga tattaaattt catgttgtga tttacagtga
     4381 aaacaatgga ccgaagcggt ttgatgaaga aatcatgcct atcgttaatg aaacatgtcc
     4441 acatattgaa gtatatgtcc taacaagcgg tcaacaccct aatttagtaa atgttaatct
     4501 tgaaggtatt aatattaata aagatgaatt tgaaatatta gtaaatgcgt tataaaaaaa
     4561 tagagcatcc tccacgttat ggaggtgctc tgttttttat tgaaaagtat caagttaatt
     4621 aatttaatat gcttaataag ttctaccttg acctttttct ctagcttctg ttcgatctct
     4681 tatgtactca gtacatactg gattttctgt taattcatca actgtattag tttaatcaga
     4741 agacgtgtct actttgtaag cttctaagaa cttattatat gatatagcgt ttgagttttg
     4801 ttgttcttct ataaatttct gtagttattt ttcaaaaacc gcatcattaa ctgataagca
     4861 gaagcgtatc acaaataaaa ctaaaaaata gattgtgtat aatataaaag gaagggattt
     4921 atattaaaat tttgaattca aaaattattg aaagggaagc taccttagaa attgaatcta
     4981 tggccactaa ctgctttttt atatcaccac ttattaaaaa atgtaagaat attcctcagt
     5041 gctaagtgaa ggaggttcta gttttattta atattcatat tgataggtaa tggttaataa
     5101 ttgcctgtaa attattgtta aatatcgttg ttatagtatc atcattcata aaatttttat
     5161 agattttatc aagtatttcc tcatcttcaa tagctgtaaa gcgatgtact agtcttttta
     5221 tgagctatat ataattttgc gaagctaaac tgtttgtaat tattcaccaa tcaggttatg
     5281 tagtgtcacg gttcataggt aattctcttt ttaaaatcat ctaatatttt atataattca
     5341 tataagtgta gtattaacgt tttctacttt atctataaaa tatttagaat tacattagaa
     5401 ttttaaatta tacggctgga aattcaatgc tgtaaaaata gacagatttt aaaaaagtag
     5461 taataaaaag tctaagtttt atctttgtaa taaacttcga ctatatttaa aaataaatat
     5521 gaatgtcttt taatttctat aatatcaaag ctattatatg atttgaaatt ttgtaacttt
     5581 ttctctaatt tggaagtaga taatgactta aaggttttaa cttttaccca acgacttggc
     5641 attttatcac tcctatgtaa tgtattactt caatataccc ttaaattttt ataattaatt
     5701 atattgtaat attattttat tttataaaaa tagtttaata aaatgtttaa tttttataaa
     5761 aaataattaa atattataga cattacgata gtttaattag aaaatatatt taagaattat
     5821 gaatttagtt cttattaatg ttaaagatca tcaattattt tgtcacatta tcttgagagg
     5881 attaccttat gaggtagcct tttatttgtc acctaatatt gctctaaggc taataataca
     5941 tatataaaat aaataattat cagaatatat ttagaaattg atatttaata attaaataaa
     6001 agtatgctaa atatttataa tttacaagtg agctgataga aagatgagta taagcgaata
     6061 taaaaataga tacttagagt cactatataa cacctttaaa actgagttag aaaacgaacg
     6121 cactcgctat tttatattag ataccaacta tacaaatagt gatgatactg aaaaggagtt
     6181 tacttgggat gtaaaacaga ataataagat cagagaaggc gatttgttca tttttagacg
     6241 gccaactaat ttatctcaaa ttgctcaaca attttatttc tttggagctg ggaaaataga
     6301 aaaaattgag cgaaaaaata atgtagcaac cgcttacatt tcaaaaccct tactttttgt
     6361 ggatcgtgtt ttaaaagata atattgaaaa tttaaaatgg gagttcaaag aaagaattaa
     6421 agaagagtgg gagcagtttt tctttaaaaa taaaatcagt aatattacaa aaaatgattt
     6481 tttaaaacta ctaagtttga gtagaaatgt agtggaaata gaaagtaatt atattaataa
     6541 tgatatttcg ctaactttta aacaaatggc tgatatggca actcgaataa agaaaaatat
     6601 ttactacgta gggaatcatg aagcatttgt tcaagctaag ggaagtgctc attttgagtt
     6661 ttcaaaaagg attaaacaaa attatagtta tagatgtgca attaccggga ttaaaactaa
     6721 agacttttta gtcgttacac atattattcc ttggcatgaa aatgagttta ttagactaga
     6781 tccttcaaat ggtatttgtt tgtctttatt tttagcaaaa gcatttaaga aaggctttat
     6841 cactttttct aacagttata gagttgtttt atctaaagaa gcagagaaag atgctgcttt
     6901 atatgaagaa ttaaagattt atgaaaatca aaaaatcgaa ctaccagatt gtcagaaacc
     6961 taatttgaaa tatttagatt ggcatagaga acatattttt aaaaattaat tttgggagag
     7021 gttgtatgac agatattagt caattagtag aggtgccata catctttcat tagtagattg
     7081 aaaaagagca cttgaaacag ataatttgta ttatttagcc tatggattaa caactttaga
     7141 taattgtttt aaatattacc aattaattac acaaaatgaa catttttgct attctcaaca
     7201 agaagaaaga gaaagttatc agcaaaaatt tcaaagtatg attaatggtt taaaaattaa
     7261 aattggcata caaagttcaa atgatgatgc gcaataagct acaaagtttg attttttaat
     7321 tgaacgttat atgtttttaa aagaagaaag agcttcgaat taatatctta atggaggaag
     7381 attagtaaat ggctagaacg tataatgtga ttaaatatgt cattaagaaa aataaggatg
     7441 atcttaaaat acatggatta agattatatg aatttaatat aaaagatgaa aaagctataa
     7501 atattagcca acccattttt gtgaacagag caacattgat tgagaaaata caaaatggtg
     7561 aagcatttac gcacgccttt agagtaaatc acaatactta ttatgcaggc caattaattt
     7621 ctttatcatt gaataaaaat ggttatatta ataacaactt tgaggcctca cgagatatta
     7681 ttagaaacat tgaagtaagt attttagact atgaataaag gtaatactga tagaatacat
     7741 tacaattaaa aaagggagta taggactccc ttaataagtt gatatataag tgacttattt
     7801 ttttaataga attttaattc ctctgttaat gttcaagata tataatatta aagcaattaa
     7861 aaagaatata atagtaataa ttaaaagaat gctaaatatt aataattgat gatccaaagg
     7921 tttatcaaag acttctttag aaaaaataaa tgccaatcta cctataaaaa aactaaccca
     7981 agtcataata tgattaatga atgctttttt gccatgttct gatgttggtt catcagcaag
     8041 tatccataca aataaaggta aaatgacagg tgcgaaaaag atacttaaat aactcaatgc
     8101 tgataaaacg taactattgt catttttatg catattacta tttgtatttt ccactttttt
     8161 caccttcttt acttttcttt actattataa aaacattaat agcgagctat tacaacagtt
     8221 ataagagaag cttacgatat tgtaagtatg tgttgttata atcaatataa aattaagaaa
     8281 gacctgagaa tttatattat catccacaat cgaagtgagt agcgaaattt attggaaaag
     8341 gtacttatat tgaagatatt gttaatggtt catttttaaa aagccaagac gatttgaagt
     8401 taaaagcttc tgacttcact gtcgaagata actataacta cggtgtactt atatttccag
     8461 agaatctttc tcttacagat aataataaga aattggaatc tttaataaaa actatactat
     8521 ttacaggtaa acgatatcaa tatacttatt ttaaaatttg atcttaatag ataaggatat
     8581 aatgatttaa acaatttaag agatattatt ttcacaaaaa aagaatgctt tttgttagag
     8641 gcactgaacc cttaaaatgg gaacttaata aaaacactat gttctaggct g
//
LOCUS       DANRDI010000111         8497 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.111, whole genome shotgun sequence.
ACCESSION   DANRDI010000111 DANRDI010000000
VERSION     DANRDI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.111"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..347)
                     /gene="sepA"
                     /locus_tag="PZ377_001982"
     CDS             complement(<1..347)
                     /gene="sepA"
                     /locus_tag="PZ377_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDM6358847.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFY"
     gene            complement(446..1789)
                     /locus_tag="PZ377_001983"
     CDS             complement(446..1789)
                     /locus_tag="PZ377_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6358848.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            complement(1966..2649)
                     /locus_tag="PZ377_001984"
     CDS             complement(1966..2649)
                     /locus_tag="PZ377_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDM6358849.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            complement(2668..3855)
                     /locus_tag="PZ377_001985"
     CDS             complement(2668..3855)
                     /locus_tag="PZ377_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDM6358850.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            complement(3899..4414)
                     /locus_tag="PZ377_001986"
     CDS             complement(3899..4414)
                     /locus_tag="PZ377_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDM6358851.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            4591..4851
                     /locus_tag="PZ377_001987"
     CDS             4591..4851
                     /locus_tag="PZ377_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358852.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            complement(4983..6353)
                     /locus_tag="PZ377_001988"
     CDS             complement(4983..6353)
                     /locus_tag="PZ377_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDM6358853.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(6589..7557)
                     /locus_tag="PZ377_001989"
     CDS             complement(6589..7557)
                     /locus_tag="PZ377_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDM6358854.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            complement(7554..>8497)
                     /locus_tag="PZ377_001990"
     CDS             complement(7554..>8497)
                     /locus_tag="PZ377_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDM6358855.1"
                     /translation="LFICIYLNMAIGSSKINFSDIIHYVTGHTDTKATFLLHNVRMPR
                     MIAGLFIGGALAVSGLLMQAMTRNPLASPKIFGVSSGASFFIVFVTIIIPALEYYALY
                     LGVIGAFIGGLTVYTLSGATKGMTPIKLALAGMAIHLFFSSMTEGIIILNENSNEQVM
                     FWLVGSLSSMKWDEILTILPWIIGALIVTIFIGRQLTIMELGDDIAKGLGQNINKVRI
                     IIGLLVIILTGMSVSVAGPIGFVGLIVPHIVKRYVSKNYLVMIPLTFIIGADLLLLSD
                     VLSRLITYPYESPVGIVTSFVGALYFLFITIKGVKRI"
BASE COUNT         3122 a         1567 c         1149 g         2659 t
ORIGIN      
        1 aaataaaata acgttgttgc aactgtgtaa gaaatcaaat gaaaagtcgc gcctctaaaa
       61 tatgcatttt caggcaacat ttttctaaaa taatccatta aatgctcgac tgcaaatatg
      121 atgaagtacc caaagattat ataactgcca taatataata ggttatcgta catacttcta
      181 ttatatgtaa agtcacccaa cctaaaataa ataagtattc tacttaatcc atataaacca
      241 aaccctagaa atgttaaaaa aatcgcacca gaaagtacaa aaataaagag aacaatcatt
      301 gtagttaaca agttataaaa cttatgttta agataattca caatcattgg cctccttatt
      361 aataaaatat tgaacgattt atctagtatc taggaaattt attatttctt actttaatta
      421 cactacatca attttaatca taattctatt cttttgattg agatgactgt tctttaaaaa
      481 atacgaccca taaaacaata agcccaatgc taattacagc aacagcactt aacacattgt
      541 gtaagtttgg accaaagatt cctgattgta tcgcataaag atatcccatt attgttgaac
      601 ctattgatgc cccaagattt ttagttaatc cataaaatga catcattttc ttcatttgta
      661 atgggctagt ttcctcttgg acaatcacac tatctttcgt ataaatatat ccaaaactta
      721 atcctgccaa aattaacaca aatgctataa gtaccggcaa tttaatacca aatgaaatga
      781 taatactact tactagtagc aatgtaaatg atagtaagta aataactttc cttgatagtt
      841 ttgcttcaat tcgatgtaaa ttaaaattca atgtaatcca agctacagat aaaggaaaaa
      901 taaccaatcc actttgcaat ggagataatc ctagttgttc ttgaaggtag actggaatat
      961 ataaattgaa tcccattaaa caaatagctg ttagaaggtc agttataaaa actaaagtaa
     1021 tcgaacgatt aaattcaacc acaggtaaaa atggtgaaga aacatgtttt tctactttaa
     1081 ataaacacat tgccacaacg atagctaaga taaaactcaa gaaatttaaa agtaacagct
     1141 gttggtttaa taaagcaaac ataatcaatc caataaatac ataaaatagc gtgagccctt
     1201 tagtatcaaa ttttgattta gcaactgttt cttcctctgg aaaatgaaat gtccatataa
     1261 ctaatataat ggcaatgatc gcaatcggta tatttatgta gaataaccaa tgccatgttg
     1321 caaattctaa tataccacca cctagaagtg gcccaatgat actcgaaata ccccaaacac
     1381 tgccaactat gcccataatt ttatatcgta acggaatttc aaatgctaac tttggaacaa
     1441 tttgcgataa tgacatcaat acacctgatc caaaaccttg tatcacgcgt gatataatta
     1501 gcatagtaaa attagtactt aaaccacaca taaaactacc tatactaaat aaaagtaatc
     1561 ctgcaattgc cacataaata attttaaatc tcgaaagtaa ttcgcccact ataggattgg
     1621 caattactaa tgcgataaaa tacgctgtga aaattaatga aattaaattt cctgcattta
     1681 aatcttgttt aattgtaggt aatgcaagcg aaataataga tgattcaatc gctgacataa
     1741 acataattaa tattaaagct ataacagtta tgattgactt taatcgcatg aaatacctcc
     1801 ttctatatat ttatttataa gattttattt acaaataatt ggttttcata tgtataaaca
     1861 cttttgactt tcaaatctta acgataattc tattacaata caatccccta ttagaatgat
     1921 ttatgtaata aaaaaagcgg agtttccccc agcttttcta aacgactaca taaaatataa
     1981 gattgcaatt aaatgcaata gtgatgctat tacaataaaa atatgccaaa tcatatgaaa
     2041 atatggtcta ttcttttgtg cataaaacca tgcaccaatt gtataagaca caccacctaa
     2101 gaaaatgaat aatatgaata tccatgatgt gcgaataaaa ataattggta acaagataat
     2161 acctacccag cccatcacta aatagacaat taagcttaac ttttggttaa ctttgacagc
     2221 tatggattta tataaaatcc cccaaagtgt cgttccccat aataaaataa atactgtcca
     2281 acctatccaa ccacctacaa ctgttaacaa aataggcgta tatgtaccag aaattgctac
     2341 gtaaatcata ctgtgatcga taatccttaa aatatattta tgaggtgttt cattttgcat
     2401 agaatgataa atagtagatg aaataaacat cataaagata gaaataacat aaatagacat
     2461 actcacagac gcaagcgtac catgtgcaat ataactgtgc acagccgcat agggtaacac
     2521 taataatgtt aacaaagctg caacaccatg agatgccgca ttaccaattt cttctccaaa
     2581 tgataaaggg atgatatcct taaatgtttc tacgattgtg tcattacttt ttttagttga
     2641 tttggacatc atagtcaccg cctatcatta aattaaaccc attctacgta aatcttctgt
     2701 tgcagttgca acactatctt taccttcttt atttttaaga ggtgaaaatt cttcttctct
     2761 aggaacttgt aacgtgacaa aactagttcc ataagtaaat atgtcaaaat agaacaattc
     2821 gaattttaaa gttggttgtt caataacacc aaagttctca tctaattgct tcaaattctt
     2881 aatagctaaa tgataaggta actcacgatt tacagcgttt aaaataaaac caagtttaaa
     2941 tgcatgaata ccgatattag cattgttaaa ttgatttgca acttctggat ctaattctga
     3001 atactccagt actgtatctt tacaatcgac attaactaaa cgtccaacac tttctcctgg
     3061 ttttggttga atcgttttag aagtaatatc gtaatcatgt tctaccgtaa atcctgcaaa
     3121 tagtggatcc aacactctta ctaaaacatt gtcgatatta ttcaagaaaa tatacttaac
     3181 gccattatta gacatttctt ctaagtatcc tgccttgtct aaagatttaa acacgccacc
     3241 attaccattc ggcgtttcca taatacgacc ctgttgattt aaaattaact tcccttcttc
     3301 actaagcgcc acaatattat cttgtttaaa gaaatggatt gcttcttgat catatccaaa
     3361 ataactatgt gcttcgaaat aagctaaagt ttcttcatga ttgatatcac ttgtcataat
     3421 ataccattga atcgtatgtc cagattgatg attcaatgtt tttaattggt tagcttgaag
     3481 ttcaaataaa cttacacctt caatttcaaa cgaaccttta gggcccttgt aaccaagtct
     3541 tgttccttga ccacctgcca tcaaaagtac tgcaaattgt ccttctttaa tagcttgaag
     3601 tccttgttcc tctaaacgct taatctcatc atcactcatt tgtgatttaa tatcatattt
     3661 cacttcagaa actgcagata catcatctat tgttttctta ttgatgtata aatcgttata
     3721 taatttggca ataaaatcta aatctaatga cgctaccttc tcttcaagtg cttctttttc
     3781 attgttactc attatttttt catattcaca caaatgatct tgtttatatt tagctaattg
     3841 atttttatct agcattttat gcctcctgct gagtttgtaa cctttaataa tttattttct
     3901 ataaaaactt agtattccag ttgcttatta tatcattgat gaaaggctga aataaaacat
     3961 aaactgttcc gaccattaac gctgtagcta aagataagtc tacaagtcca cctgttttaa
     4021 attgaatcgg tgtcttcaca ttaaacggta acggataaaa taatttcacg ccttttggtg
     4081 ttatcatatc tagtataaca tgtgacacta atccaattat aattgctgcc atataataat
     4141 taggcgtctg aataatttgc aataaaaatg ctatgattgc gataaataat attgaatgcg
     4201 taaaggttcg atgtccaaag atcaatctca caaaaaaact aattacctta aaccttctgc
     4261 caatcttact ttgagtgtga catatatcgg gtaataagct agctagagtt gctagaatga
     4321 taaccgtaac cgacgaaaaa atatccgttt gaaaatattg tgttgttagc gctccaacga
     4381 gcatgccgca tgaagcatgt gttttacctg tcatatttgt tctcctttaa tactcacatt
     4441 ttaccacatc cctaacaaaa acacgaacat attttcgggt taaaattcat tagtatgaca
     4501 caatttaaaa aagtatcaca taactcttga aaacgattac aaaatcgttt atgatgtatt
     4561 tacaaaatat ttaaaggatg tgtttgaata atggcaatga cagtaaaaaa ggataataat
     4621 gaagtgcgta ttcaatggag agttgctgat atcaaaattc ctacaagtga aattaaaaat
     4681 attacacaag accaagatat tcatgcagtt cctaaattag acagcaaaga tgtatctaga
     4741 atcggctcaa cgtttggtaa aacgaatcgc gttattatcg atactgaaga ccacgaatac
     4801 attatttata ctcaaaatga tcaaaaagtt tacaatgaat taactaaata aattgtataa
     4861 aaaaatcatt catggtgagg gcttcatgaa tgattttttt aattgattca acacccagca
     4921 taaacaaata caaaaggaca actgttccca taattttaac agttgtcctt tttcacatat
     4981 atttataaca aaagatgtgc catcaaagaa ataattggta gtgtaatgat tgttctaatc
     5041 aagaaaatca taaacaattt gccgatgctt acaggaatct tcgaaccaag tatgacgcca
     5101 cctacttcag acaagtatat taactgcgat atactaagtg ccccaataac aaaacgagtt
     5161 atatcatttt gtacaccttc aattaatata gaaggtaaaa acatatcggc aaaaccgata
     5221 ataatcgttt gagaagcctg tgccgcttca ggtatttgca ttaactctaa aaatggaaca
     5281 aaaggtttac ccaatatgac aaaaaagggc gtgtagttcg caataatggt agcaatagta
     5341 ccaatactca ttactacagg caaaataaca aaccacatat caatgactgt ttttaatcct
     5401 gacttaaaaa agtcaataac gcccggtgct ttaataccta cttctgttgc agtatcaaag
     5461 ccatgtctca atgccgtctt tccttctggc aatgcctcag tacgcgcact ttcaggtacc
     5521 tccttagcat actcatcagg aattttattt aaaggccaaa ttcttggcat aatgactgct
     5581 gcaacgaggc aggatactat cactgataaa tagaaagcaa aaaattgatt ttgcatgtgc
     5641 actgtttcag caactacaat tgcaaaggtg atagaaacta cactaaatgt cgttgaaata
     5701 actgttgcct cacgacgaga ataatatcct tcaccatatt gtctacttgt aattaagaca
     5761 ccaacagttc cgtctccaat aaatgatgct aaattatcta ccgtcgaacg tcctggcaat
     5821 gtaaataaag gtctcataac cggtctaaaa ataggaccca acatctctaa caaaccgtat
     5881 tccattaata gcggtaaaaa tagagctgca aataagaata ctgccactaa tgttggcaat
     5941 aaacttgaga acaataatcc acccgtttca tctgagtaaa taacctttga accaattcgt
     6001 aaaaatgtca tccatgcaaa aacaactgct aatattcgta aaattaacca accaattcta
     6061 acgttaaaag cattgttcat tagcccgtca ggtttcaatt tatcttttaa aatagttgaa
     6121 caaatcagag ttatgatacc cgataaagta attatcgtca caattaaaaa tggcattacg
     6181 ccacctaata catctttaag cacgcctgct aaaaatgcca cgggcaacgt tgtttgcttc
     6241 tgtccatctt gttcgactgg aattggtact aaaaataata agataccaat taaagacatc
     6301 gtaataaact taagtctccc aataactatc tctttccttg aaaagctatc cataaaatca
     6361 atccatttct ctatgtattc gttttaagta tatacagaat tctattcagt taacaaacat
     6421 attccttatc attctatctt tcaaaatgtt tatgtatgca aaataatgaa taattacagt
     6481 tattaaatat acgctatttc ttgtaatttt tcaagatgaa ttcaaaaaag gttaagtaca
     6541 attactgatt tcgtacttaa ccttttttaa actctaatca tatgttagtt atttcattct
     6601 tcgtaataat attaagaagt atggtgcacc gataattgca atgataaccc caacaggaat
     6661 atccagtggc ggatgaatgc cacgggctaa accatctcca aatgttaaca atatagcacc
     6721 aattaacccc gacatgataa taacgtgtaa tgttttattt cctattaatt gtctcgcaat
     6781 atgaggtgca attaatccta aaaagctaat accaccgaca actgaaattg cggatcctgc
     6841 taatattact gctaaaatta acaatagcat tttaatagtt ttaactttta aaccgagtgc
     6901 ggttgcaaca gcatcaccta gattcaatac atctaattga taactcaata aaatgatgat
     6961 tggtatcgtt attaaaaacc aaggtaatat agtataaata ttcgacatat catgtccata
     7021 tagactacct gtcaaccaaa caagcgcttt gtttgcttcc agtggatttc tgattaataa
     7081 gaactgcaca atcgccgtac atattgcgcc tattgctaaa ccaattaagg caagctttga
     7141 acctttaaca tcatattttg aaattaaaaa tgataaaaat aaacttactg caaaggcacc
     7201 taagaatgaa cctataggta atacaaacaa tggtgctgtt ggaaaggtca taataataat
     7261 cacagcagct aaactggcac ctttagaaat acctataaca tcaggtgagg ctaacgggtt
     7321 tcttattaca gcttgtataa ttgcacctga aatagccaag ctactaccga taataatacc
     7381 aagtaatgtt ctaggtatac gatactcatt taaaataaaa tcatcttgtg taaagattcc
     7441 cttaatagca tcaatcggat gaatcatgac agaccctaca cataaactta tgaatatact
     7501 cacaattaaa aggattgtga ttaaactata acgacgtata attttcgttg tcatcatatt
     7561 cttttcaccc ctttaatcgt tataaataag aagtaaagtg cacctacgaa tgatgtaaca
     7621 atccctactg gtgattcata aggatatgta attaaacgac ttaatacatc tgatagtagt
     7681 aacaaatctg cacctataat aaatgttaat ggaatcatga ctaaataatt cttactcaca
     7741 tagcgtttga ctatatgcgg tacgattaag ccaacaaatc caattggtcc tgccactgac
     7801 accgacatac ctgtaagaat aatgactaat agtccaatga taattctaac tttatttata
     7861 ttttgaccca atcccttagc aatgtcatcg cctagttcca ttatcgttag ttgacgtcca
     7921 ataaaaatcg ttacaattaa agcaccaata atccatggta gaatggttaa aatttcatcc
     7981 cacttcatac tggataatga accgacaagc caaaacatca cttgttcgtt tgaattctca
     8041 ttcaaaataa taataccttc ggtcatacta ctaaaaaaca aatggatggc cataccagcc
     8101 aatgctaatt taatgggtgt catacctttc gtagcacctg aaagtgtata aacagttaaa
     8161 ccacctataa atgcacctat cacacctaaa tataatgcat aatattctaa tgctggaatg
     8221 ataatcgtca caaatacaat gaaaaacgat gcaccagaac tgacaccaaa aattttaggt
     8281 gatgctagcg gattgcgtgt cattgcttgc attaacaaac cagataccgc taatgcaccg
     8341 ccaataaata accctgcaat catccttggc atacgtacat tatgcaataa aaacgttgct
     8401 ttcgtatctg tatgaccagt aacatagtga atgatatcgc taaaattaat tttcgaagaa
     8461 ccaatcgcca tatttaaata tatacaaata aaaagaa
//
LOCUS       DANRDI010000112         8113 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.112, whole genome shotgun sequence.
ACCESSION   DANRDI010000112 DANRDI010000000
VERSION     DANRDI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.112"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..736)
                     /locus_tag="PZ377_001991"
     CDS             complement(<1..736)
                     /locus_tag="PZ377_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDM6358856.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIP"
     gene            complement(953..1858)
                     /gene="yut"
                     /locus_tag="PZ377_001992"
     CDS             complement(953..1858)
                     /gene="yut"
                     /locus_tag="PZ377_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDM6358857.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            2118..2420
                     /locus_tag="PZ377_001993"
     CDS             2118..2420
                     /locus_tag="PZ377_001993"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDM6358858.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            2434..2844
                     /locus_tag="PZ377_001994"
     CDS             2434..2844
                     /locus_tag="PZ377_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDM6358859.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            2841..4556
                     /gene="ureC"
                     /locus_tag="PZ377_001995"
     CDS             2841..4556
                     /gene="ureC"
                     /locus_tag="PZ377_001995"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDM6358860.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            4569..5021
                     /gene="ureE"
                     /locus_tag="PZ377_001996"
     CDS             4569..5021
                     /gene="ureE"
                     /locus_tag="PZ377_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDM6358861.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            5014..5703
                     /locus_tag="PZ377_001997"
     CDS             5014..5703
                     /locus_tag="PZ377_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDM6358862.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            5716..6330
                     /gene="ureG"
                     /locus_tag="PZ377_001998"
     CDS             5716..6330
                     /gene="ureG"
                     /locus_tag="PZ377_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDM6358863.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            6330..7166
                     /locus_tag="PZ377_001999"
     CDS             6330..7166
                     /locus_tag="PZ377_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDM6358864.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(7355..7702)
                     /gene="sarR"
                     /locus_tag="PZ377_002000"
     CDS             complement(7355..7702)
                     /gene="sarR"
                     /locus_tag="PZ377_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDM6358865.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
BASE COUNT         2788 a         1337 c         1337 g         2651 t
ORIGIN      
        1 ttggtatatg caatatccaa ccatttggcg ctttactttt ctctgtttcc actaaatgtt
       61 ttaatggtac ttttacatca tttaatatta aatcatgact ttctgtcgct cgcataccta
      121 atacgtccca attatcggca atttcaacac caggcaaatt cctgtctact aagaaaaaac
      181 caacactttc taattcttct atataagcag caacaataat atgtgttagt gctttactca
      241 ttgatgtata tgtcttcaca ccatttaaaa tatacccatc atcagcttta acagcatgtg
      301 tacttggtct tccccctctt gttggactac ccatttcagc ttcactaact gctctattaa
      361 ctaatgcacc attattaatt tcaacagcaa attgctccaa catatcttga gaccataatt
      421 tctgttcata aatttgtcct acgacactca catgccaacc aatagataat gctgtggcac
      481 catcaagttc gcctaaaaat gattgcaaaa taaccatgtc ttctatggtc gcaccttcac
      541 caccatatgc ttttggtaac gttaattttc cataaccttc tttaattaac cattcaatat
      601 ttttatatgg aaattggcta tgttgatcat tatattcagc tttagcctta aatgtatctc
      661 taattacttt gaatttttca atccattttc tttgaatttc cgttgttatt aaaaaggaat
      721 tatccatatc attcatatta gtcaccccac acaacacaca atataaatta tttattccta
      781 gtttaacact cgactttaaa aataaaaagg aacgtgctgt gaacaattaa aagaaaactg
      841 ataattcgat taagtatgca cttaatttta tccataaaaa tacaccttaa actatacaat
      901 tcgctgtata gtttaaggtg atattcaaac aatattaggt ttgatttaac tatcaagcat
      961 ctactttgtc atgtttaata ccagcaaata ataaaatcca tgtcacgata ataaatggca
     1021 atgttaatgc aggtaagcca aacggttcaa gcaatgttgt tgtacctagt tgcaccacta
     1081 ctgttaataa cacacctaaa aaagtcgaaa tataagggtt aatcgctgtt ttaaatgtat
     1141 atcctagtgc gattgccatt aatacaaagt tataaccgaa taatccctga ttaatatcat
     1201 cataattacc acctaataga gctacaatga taaagcttaa caaactagct ataacagcta
     1261 agagtgttgc ttttcttgat gctatcaata tgccgattaa aatacatacg ccaccaatta
     1321 cactcgcttc gataaatact tgactgaatc cttcaaataa tgactgaatg aaatgtattc
     1381 tatcattgtt gctaaaatta accgtttcaa tgttttgagg cattaacttt aacgatgtat
     1441 ctacaaattt aacctgtcct gaaagtaaaa ttgtaaacca agtcacaatg acaaaaggca
     1501 tcgtcagcat cggaacttta tatggtctta aaacttctct cactgcagca gcaactggta
     1561 acgttaataa agttgctatc attgttataa caatatctaa tcctgactta tctaaaaaga
     1621 ttgttaacgc aatggcagtt agcactggat taaatccagc taacccatca ttaatctctg
     1681 cctcactata atttataaaa cgcgcaaaag tataggcgat gatgctacct acaatagccg
     1741 ctaatccaat tgtccaatcg gctacaaata atcctattaa gataaataat cctgtccatt
     1801 tattactaat taacacaacc tgagatatat ttttcaaaag cacatctatt atcttcaaat
     1861 ctttcacctt tcctttgaat cattactcat taatatacta ctattctatg aaaagttata
     1921 ttaaaaacta ttttggtttt tacataaata tattccacta tttgttttcg attagattgt
     1981 ttgcttcaat atcggatatg ctaaaaatat aattttctaa ttacacattg aactttgtat
     2041 attacttgtt tatcacaaca attaggatta atataaattt tgtaaattgt atctaatatg
     2101 aaattggggg ttttaatttg cattttacac aacgagagca agacaaatta atgattgtag
     2161 tggcggcgga agttgcacgt cgtcgtaaag cacgtggttt gaaactaaat catcctgagg
     2221 cattagcttt aatcagcgat gaattattag aaggtgcacg cgatggtaag accgttgcag
     2281 agttaatgag ttatggtaga caaattctaa acaaagaaga tgtcatggat ggtgtcgaac
     2341 acatgattac agatatcgaa atcgaggcta cgttccccga tggtactaag ttaatcacag
     2401 tacatcaccc tattgtttaa aggaggcagt caaatgatac caggagaaat tattacaaaa
     2461 agtacagagg ttgaaattaa taaccatcat cctgaaacag ttatcgaagt tgaaaataca
     2521 ggagatcgac caattcaagt gggctcacat tttcattttt atgaagcaaa tgcagcatta
     2581 gatttcgaac gtgaaatggc atatggaaaa catttagata ttccagctgg agcagctgtt
     2641 cgatttgaac ctggggataa aaaagaagtt caattagttg aatatgctgg caaacgtaaa
     2701 atttttggtt ttcgtggtat ggtcaatggt cctatcgatg agtcacgtgt ctatcgccca
     2761 actgatgaaa atgatgaata tgcaggtgta ttcggagata acggtgctga aaacgtgaat
     2821 aaaaaaggag gaaaaagatc atgagcttta aaatgacgca aaatcaatat acgagcttat
     2881 acggtccaac tgttggagat tccattcgtt taggtgatac gaatctattt gctcaaatag
     2941 aaaaagacta tgcggtttat ggtgaagaag ctacttttgg tggtggtaaa tctattagag
     3001 acggtatggc gcaaaatcct cgtgtaacac gtgatgacgt gaacgttgca gaccttgtca
     3061 tttctaatgc cgttattatc gattacgata aagtggttaa agctgatata ggcattaaaa
     3121 atggttatat tttcgccata ggtaatgccg gcaacccaga tataatggat aatgtcgaca
     3181 ttattatagg ttcaacaaca gatatcattg ccgctgaagg taaaatcgtc actgctggtg
     3241 gtattgatac tcatgttcat tttattaatc ctgaacaagc agaggtcgca ttagaaagtg
     3301 gtattacgac tcatattggt ggtggtactg gtgcttcaga aggttctaaa gcaacaactg
     3361 taactccagg tccatggcat attcatagaa tgttagaagc tgccgaaggt ttaccgatta
     3421 atgtcggttt tacaggtaaa ggacaagcaa caaatccaac tgcactcatt gaacaaatca
     3481 atgccggagc aattggatta aaagtacatg aagactgggg tgcaacacca tctgctttga
     3541 gtcatgcatt agatgttgct gatgaatttg atgttcaaat tgcattacat gcagatactt
     3601 taaatgaagc aggatttatg gaagacacaa tggctgctgt taaagaccgt gtacttcata
     3661 tgtaccatac tgaaggtgct ggtggcggtc atgcgcctga tttaattaaa tccgctgcat
     3721 tttcaaatat tttaccttca tctacaaatc caactttgcc ttatacacat aatactgtag
     3781 atgaacattt agatatggta atgattactc accatttaaa tgcggctatt cctgaagata
     3841 tcgcattcgc agattcacgt attcgtaaag aaacgattgc agcagaagat gttctgcaag
     3901 atatgggtgt attcagtatg attagttccg attcacaagc aatgggccgt gtaggtgaag
     3961 taattacacg aacatggcaa gtagcacatc gcatgaaaga acaacgtggt cctttagatg
     4021 gtgattttga acataatgat aataatcgca tcaaacgtta tatcgctaaa tatacaatta
     4081 acccagcaat tacacatggt atttctgaat atgtaggatc tatcgagccg ggcaaactag
     4141 ctgacattgt cttatgggac ccaattttct ttggggttaa acctgaatta gttgtaaagg
     4201 gcggattaat taactctgcc gtaaatggcg atgcaaatgg ttctatacct acatctgaac
     4261 cgatgaagta ccgtaaaatg tatggtcaat acggcggaaa ccttacaagt acgtcaatga
     4321 cattcgtgtc taaaactgct tatgaaaatg gtatcaatcg tgcattaaat ttaaaacgca
     4381 tggtgcgtcc agttaaaaat attagacaat tatctaaagc agatatgaaa aataacagtg
     4441 caacacctaa attagacgtt gatccacaaa catatgaagt atatgtagat ggagaaaaaa
     4501 ttacaagtaa tgcagcaact gagttaccat taactcaaag atacttctta ttctaggagg
     4561 aacatagaat gattgttgaa gaaatccaag gcaatattgc caatttgtca aattcagaaa
     4621 aacaaaagca cgtcgaaaaa gtataccttg aaaattcaga tcttgttaaa cgtattcaaa
     4681 gagtcgttac agaccatggc actgaaatag gcattcgttt aaaacaacct attgacttac
     4741 aatatggaga tattttatac gcagatgacc ataatatgat tattgtggat gttaattcag
     4801 aagatctttt agtgattcaa ccaagaacat tgcaagaaat gggagatata gcccatcaat
     4861 taggaaatcg tcatttgcca gcacaattta cagagactga aatgctagta caatatgatt
     4921 atttagttga agatttatta aaaagtttag gcatcccata tgttcgagaa gaccgtaaag
     4981 ttaataaggc tttcagacat atagggcatt ctcatgattg atcatacaca cttaagatta
     5041 tttcagttct gtgattcaca gtttccaaca ggtgctttca gtcattcatt tggtcttgaa
     5101 acatatattc aacgaaatat aattcatgac gatcatactt ttattgcttg gttaaaaatg
     5161 tttttacaag agcaacttac ctattctgat ggtttagcca tgcgtttagt ttatgatgca
     5221 ttggaaaatg atgatacaca aaaagtatta cacatagata aactgatgtt tgttcaaaac
     5281 ttacctaaag aaacacgtgt tggtgcaaaa caaatgggga ctcgcatggt taaattggct
     5341 ttagaacttt ataatagccc atggattgct tggtatcatc aacaaatgca agataagaaa
     5401 gcaaagttaa atccagctat ttgttttact atgctaggcc atcatttagg tgtagatatt
     5461 gaaacgatta ttgattacta tttatatcaa aatgtttcaa gtttaactca aaatgctgtt
     5521 cgtgctattc cacttggaca aactgctggt caaaagatag ttactcacat gattccttat
     5581 atagaaggaa caagaaagca gattttcgaa ttaaaagaag cagattttgg catgacagcg
     5641 cctggtttag aactaaatca aatggcgcat gagaacgtca atgttagaat tttcatatca
     5701 taggaggtta taattgtggc aaatccgatt aaaattggta ttggtggtcc tgtaggtgca
     5761 ggtaaaacac aattaattga aaaagttgta aaacgtcttt caaaagaaat gagtatcggc
     5821 gttattacaa atgatatata tacaaaagaa gacgaaaaga tattagtaaa ttcaggagtt
     5881 ctacctgaaa gtcgtatcat tggtgttgaa actggtggat gtcctcatac tgcgattcgt
     5941 gaagatgcat ctatgaactt tgcagcaata gacgaattat tagaacgtca tgacgatata
     6001 gaactaattt tcatagaatc tggtggcgat aacttagcag caacatttag tccagaactt
     6061 gttgactttt caatatatat tatcgatgtt gctcaaggtg aaaagattcc acgtaaaggt
     6121 ggtcaaggta tgattaagtc agatttcttt gtaattaaca aaactgattt agctccctat
     6181 gtaggtgcat cattagaaca aatggctgaa gatactaaag tatttcgtgg taaacgtcca
     6241 tttactttta ctaacttaaa aaccgacgaa ggtttggatg aagttatcga ttggattgaa
     6301 cgcgacactt tactcaaagg attatcataa tggatgaaca acaatggact gggcaacttg
     6361 atttaacagt gtttttcgat ggcaatcgat cagtatcaag agatattttc tttgaaaaag
     6421 cacttaaagt gatacgtcca gtttatctaa atcaatctac cattcctaca ttttatatag
     6481 taaatgtagg tggtggctat ttagatggag atcgttaccg tatgaatgtg aatgtcgaag
     6541 ataacgctaa agtgacattg acatctcaag gtgcaacaaa aatatacaag acaccttcca
     6601 atcatgttga gcagtatcaa acttttaatt tgaaagataa cgcatattta gaatatgtcg
     6661 ctgatccaat catcgcatat gaaaatgcta aattttatca acacaatacg ttcaatctca
     6721 ataattctag ttcattattt tatactgata ttttaactcc tggttattca aaaactggcg
     6781 aagccttcaa gtatcaatat atgcatttaa taaatgaaat ttatattgaa gatgagttag
     6841 tcacatatga taatttatta ttgaatccta ataaacaatc gatcaatgaa ataggttata
     6901 tggaacatta ctctcattat ggctctgctt acttcataca cgaagatgtt aaccaaaaac
     6961 taatcgattc agtttatgaa accattagtt cttatagcaa tacattcgat tgtcgtgttg
     7021 ctatttctca attgcctaca catggttttg cagttagaat ttttgcttat cgcacacaaa
     7081 ttatagagaa aatacttgga accatccaaa gctatatcgc agaaaatatt tatgatcgta
     7141 aacttgattt tctaagaaaa tattaaaata aaaagactag tgtaccttgt ttcaagctta
     7201 ataagctgaa ataaggtatg gctagtcttt aactttaatt aaagcatctt caatatttaa
     7261 attttaagtt aattttatgt cccaatgctg aataaataac ttcgttttaa taaattgtta
     7321 tcgttcaatg ttattaaacg caattaacct tgatttaatt tttaatgtat tcttctaatt
     7381 ctgaaatcag tttttgaata tttgcttttt gtgtatctgt aacataaaca ataactgttc
     7441 tttcgtcttg taaacttctt ttctttgata acaattttaa atcttttagc ttttgtaaag
     7501 ctttagttaa atagtaaggt ttgaactctg agcacttagc aatctcttta gatgagattt
     7561 cgttagactc acttcttaaa atatgattta aaatataaat ttcttcatag ttcaaattga
     7621 acttcttttt tgtatctctg aaaaacttct taacttgaaa tgttgcgttg actaaatcat
     7681 taatgtcatt aattttactc attattaaac cactcctctg atgcacatct tgttgtagca
     7741 aggttcacat gtaatttatt aaatcgaaac tctatccaac tatgttataa agttcattct
     7801 aaacaaataa gtataaaaat tcaatatttt tattactaga atatggttaa atacatttat
     7861 ttcttataat aatttgatgt ttaagatatt ttgccaaatt gaaaattatt agtctgaata
     7921 ttttgtatct taactgatat gtaaactttt aaaattttaa tctatatcaa cgtgtttgat
     7981 ttgatatttt ttatattaat tggatggctt gctcacaaaa tttataaaat ataaaaaaag
     8041 aagagcttta aatcataacg catgccattt atctttaaat gcaatgagtt atcattcaaa
     8101 ctcttcttat caa
//
LOCUS       DANRDI010000113         8070 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.113, whole genome shotgun sequence.
ACCESSION   DANRDI010000113 DANRDI010000000
VERSION     DANRDI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.113"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            232..417
                     /locus_tag="PZ377_002001"
     CDS             232..417
                     /locus_tag="PZ377_002001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358866.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(640..1218)
                     /locus_tag="PZ377_002002"
     CDS             complement(640..1218)
                     /locus_tag="PZ377_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDM6358867.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1482..1862)
                     /gene="blaI"
                     /locus_tag="PZ377_002003"
     CDS             complement(1482..1862)
                     /gene="blaI"
                     /locus_tag="PZ377_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDM6358868.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(1852..3609)
                     /gene="blaR1"
                     /locus_tag="PZ377_002004"
     CDS             complement(1852..3609)
                     /gene="blaR1"
                     /locus_tag="PZ377_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDM6358869.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            3716..4561
                     /gene="blaZ"
                     /locus_tag="PZ377_002005"
     CDS             3716..4561
                     /gene="blaZ"
                     /locus_tag="PZ377_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDM6358870.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5087..5506)
                     /locus_tag="PZ377_002006"
     CDS             complement(5087..5506)
                     /locus_tag="PZ377_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDM6358871.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5630..6601
                     /locus_tag="PZ377_002007"
     CDS             5630..6601
                     /locus_tag="PZ377_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDM6358872.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(6773..6865)
                     /locus_tag="PZ377_002008"
     CDS             complement(6773..6865)
                     /locus_tag="PZ377_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDM6358873.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7022..7369)
                     /locus_tag="PZ377_002009"
     CDS             complement(7022..7369)
                     /locus_tag="PZ377_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358874.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7388..8005)
                     /gene="cadD"
                     /locus_tag="PZ377_002010"
     CDS             complement(7388..8005)
                     /gene="cadD"
                     /locus_tag="PZ377_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HDM6358875.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         2874 a         1167 c         1057 g         2972 t
ORIGIN      
        1 tataaattca actttgcaac agaacctcat ttactatatg ggcatctctt tcattattaa
       61 tacaaagctt tgtatctccg atattaggca aaggtgtaaa cgaattaggt gatactatag
      121 gattcaacat tatattaatc gtttctttaa taatgattat cttcttattt tttataaatc
      181 gtagtaaaaa tatctcctat gattatataa aagttcaaag aggtgaagcc aatgaataaa
      241 ttagaaaaat ggtctttggt atttattatt tttacatttc tctcttcatt agcactttta
      301 tatttcaaat ataattttag ttcggttatg ccagtcctaa gtctttctat tttaactata
      361 ctaatattgt tgacgagtat tattactgta gtttcagtta atgatgaaga tacttaaaac
      421 gttcattagt gaaacaggta ttatattaaa aaagcgtacg aatttgtacg cttttttctt
      481 gtgttattat taaaatctca taacaggaaa atattatgag attttagacg ctgtcagaac
      541 gtctctttta catcgagtga cattgaatca aagtctttgt atgttaatga tatataaaat
      601 tatttagtag attcattata gtaagctaga ttagttgctc tacttgtttt tattctcttt
      661 tgcctctaat ttattgagat aacgataaat agttgtacgt gaaacttgcc actgctctgc
      721 aattgtttta attggcgtac cattatcata aagtgtctta agtaagttta aatcttgttt
      781 atttaatttt tcaggtctac ctccatatcg gcctctagca cgtgctgcaa ttcgaccggc
      841 tgatgatcgc tctaaaatta aattacgttc aaactctgca aatgctgcga ataaatgaaa
      901 taataattgt cctgtagaac ttgatttatc catcgttata ttttcttcta aactatgaaa
      961 actcacacct cgttcgttaa gttcattaac taacgtgatt aaatcctcca tgttccgtcc
     1021 tagtctatct aatctccaaa caacaatcgt atctcctgac ctcgcaaatt caattgcttt
     1081 atctaaacca ggtcgcttac tttttgaacc actcatatgg tcattaaata tcttttcaca
     1141 accatatgta tttaatcgat cctcctgtaa attcaaattt tgtaatccag ttgaaactct
     1201 tgcataacct attttcaaaa tacagacctc ctatatacac ctttagtgta ccataacttg
     1261 attacgaggt cttttgtgaa catagatttt ttgttgggtt tttgaacaca taattctatg
     1321 taatacttac ttatttagac aaaaaacaag agtgtacaga aaatggttat tttctggaca
     1381 ctctcatctt taataatata ttttaaatgt cattaacaaa actaatttaa taagagtcaa
     1441 gtatggttta catatgtatt atgtttgaaa catacctcta attacttttt actaatatca
     1501 tttaaaatgt ctcgcaattc ttcaatttct ttgttattta attcttcatt tttcgcaaaa
     1561 ttcagcacta aacttttcat atcccctcca tacagtttat taagaaaggt tttagcagtt
     1621 ttcattttaa tatcgtcttc tttaatattt gatgagtaaa aataaatatt ttctgatttg
     1681 tatcgtttta taatctcttt tttatatagt cttgtgatta atgttctaat cgttttatca
     1741 ctaacttctt tatatttttg aatttcaact acaatttcat tagctgatac tgatttttta
     1801 tcccatatta tattcataac atcccattca gccatagata tttcaacttg cttattggtc
     1861 atttaaaaca cccattcctt ttaatatttt ttcacttatt aattcagcat ttttcccaga
     1921 cgggtttcca tctgataaat gtgtagcaaa ataatactta tcatgatttg taattacgta
     1981 acctacaaac cacccattat tatacttccc gtttactata cctgtacctg ttttcccata
     2041 cagttcatac ttttcatttt tcttaatcaa taatgaagaa gataattgat tctttgcttt
     2101 tttactaaaa tggttatttt gttccatcat atttttaaaa actattactt gttcaagatt
     2161 agatattttc aaactatctt ccatccaata gcttttataa cttcccaaat tttcattacc
     2221 ataatttaat tgcttgagtt gagccgcagt atagttcttt ggtatttgat tactaatacg
     2281 ttcgaagtac caattaactg aattttgcat tgctgtattt aaatcttgtt ccttattcca
     2341 agactcgaaa agataatgtt tatgattcca tgacatacgt gaatttttat cacttataat
     2401 atgacggtca agtccaaaca gtgctaaata aattttgtaa gttgaatcag gagaatatct
     2461 ttttctactt tctttttcat tataaatata gtatttgtct ttcttcatgc tgtacataac
     2521 gaaagatcca ctatttgaac caaaattttt actttcatct aaaatttggt aatcgctttg
     2581 taagggcttt ttataattat aatcagttaa ggattgcccc attaaaaact ggctttgaat
     2641 tatcatgata aaaaaagtaa aaatacaaat aaaaattaga attaattttg actgcttttt
     2701 cagatcggct tcttttatat taattaatct acttttgagt aatgattttt taccattaaa
     2761 tgaatggctt aatatatttt tgttggaaaa tggggttttt aatacggagt ccataataga
     2821 ttccgcataa gttttaaatt catttttgtt aatattatta aggacaaatc tatcggcttc
     2881 tacttcattg tcatgaatta tctttctttt tacaatatgt actaatggat tgtaactcat
     2941 aataatagag aagatattaa agataattaa atgtaaagta tctctatttt tagcatgagc
     3001 atattcatgc agtattatat attttagtct tttatcaatt acacttttaa aatatgaact
     3061 aggaatcaaa ataatatatt tcccatacca aaaagttatt ggagattgaa tagcctctgc
     3121 ttttcgaatc acaatatttt ttttatattg atggttgaaa agtatcgtat ctattttatt
     3181 tttttcattt tcgtttagat aaagtgactg tttctttaaa tatttaagat ataataaggc
     3241 tttcaaaaat ttaaaactta aaataataac taaaactatc caaataactg tgcagatatt
     3301 atcaattgaa tcccaattaa acttatggat atctgttgtg aactcttgaa taggtttggt
     3361 ggtatttatg ttatggttca aatcgtgtga cttactttca actgtgggcg cttgattatt
     3421 cacattatta aattttataa aagagaattt aattgggata aaaggaatta atccagcaag
     3481 catagttaga taccaaactt tataatttaa actataatta aaatagcgtt ttaatatgta
     3541 cctaaaaaat actaacaata gaaatataaa acaaaagctt actacgctca ttattagtaa
     3601 tttagccatt ttgacacctt ctttcaaata tttataataa acaattgaca tcaatattac
     3661 aattgtaata ttattgattt ataaaaatta caactgtaat atcggagggt ttattttgaa
     3721 aaagttaata tttttaattg taattgcttt agttttaagt gcatgtaatt caaacagttc
     3781 acatgccaaa gagttaaatg atttagaaaa aaaatataat gctcatattg gtgtttatgc
     3841 tttaaatact aaaagtggta aggaagtaaa atttaattca gataagagat ttgcctatgc
     3901 ttcgacttca aaagcgataa atagtgctat tttgttagaa caagtacctt ataataagtt
     3961 aaataaaaaa gtacatatta acaaagatga tatagttgct tattctccta ttttagaaaa
     4021 atatgtagga aaagatatca ctttaaaaga acttattgag gcttcaatga catatagtga
     4081 taatacagca aacaataaaa ttataaaaga aatcggtgga atcaaaaaag ttaaacaacg
     4141 tctaaaagaa ctaggagata aagtaacaaa tccagttaga tatgagatag aattaaatta
     4201 ctattcacca aagagcaaaa aagatacttc aacgcctgct gctttcggca agactttaaa
     4261 taaacttatc gcaaatggaa aattaagcaa aaaaaataaa aatttcttac ttgatttaat
     4321 gtttaataat aaaaacggag acactttaat taaagatggt gttccaaaag actataaggt
     4381 tgctgataaa agtggtcaag caataacata tgcttctaga aatgatgttg cttttgttta
     4441 tcctaagggc caatctgaac ctattgtttt agtcattttt acgaataaag acaataaaag
     4501 tgataagcca aatgataagt tgataagtga aaccgccaag agtgtaatga aggaatttta
     4561 atattctaaa tgcataataa atactgataa catcttatag tttgtattat attttgtatt
     4621 atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctttatta ttttcgagat
     4681 ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa atcataaata
     4741 atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta tcttatttaa
     4801 agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca agcaaagtga
     4861 caatagcatt ttacaagtat tttttattgt tcgattaggg taaacctaaa taatacataa
     4921 attatattac aatatgttta ttatagaggc taaagtatct tagaaatttg gatagtaggt
     4981 ttttcgggaa taaaagtata gttggcttta atatataatt aagctaagct atcatttata
     5041 tttactcgat tataattcgc acttcaactt ttttataacg taaaaattaa aacgtagttt
     5101 tatttatctt tagtaatatc tttttaaaca ctacagtttc ttcattagtt aaattagcga
     5161 agtaatgatt attaagttga taggctttct tttctaatgt tggccataag tcttttgctt
     5221 tatttgtact gtttattagc acagttcttc tattttcatt atcagtttga cgtgttatta
     5281 gatccttttt ggacatctta tctaatattc cagatattgt tgaaggttct aaatttaaag
     5341 tattaccaat cgctttaggt gtattcaaat tactattaat caagcaattc aaaacaccat
     5401 attctgctgg agtaatatta tagggactta ataaactttt aaaatgttta aaaactctat
     5461 tctgagaagt actcaacaaa taatttatac aattatctaa ttccacaact aaaacctctc
     5521 ttaaatcatt agtattgact cgcttatcat atcatgataa gttttgtagg aaaatagttt
     5581 tgcaacaaaa acatacttct acaaaacaat tgtgaaagaa ggctaataaa tgttcaatgc
     5641 ggtagtagtg agaaaaagtg catcaaatgt tagttataaa gtagaaaaag ttgatgaatc
     5701 tcatttatcg aagggtgatg tattagtaaa agtggtatac tcttcaataa attataagga
     5761 tatgctagct ttacaatata aaggcggtgt aattagagat tatcctatga ttcctggtat
     5821 agattttgca ggtattgttg aatcaagttc gaatgataag tttaaagaag gcgataatgt
     5881 attagttact ggttatgatg ttggcgtgac acatacaggt ggattttcag aatatgcaca
     5941 agtgcctgga gagtggattg ttcctattcc ccaagaaatg tctattaaag agtctatggt
     6001 atatggcaca gccggtttta cggcagcatt atcaattttt gaactagaaa aatatggaat
     6061 gaatatcaaa tcacaacctg aaattttagt gtctggggct acaggtggtg tgggcagtgt
     6121 tgctatacaa attttacata aaattggata tgaaaatatc actgctatcg taagaaaaga
     6181 aaagcaaata gaaatcgcaa aaaaattagg tgcaaaacaa gtaataataa ttgaagatga
     6241 taataaaccg cttaaaaaag gtatttttga ttatttctta gatacagtgg ggagtaatgt
     6301 aacattatac ggtttaaaac gtctaaatta tcaaggtgtt gctactgtat gtggaaatgt
     6361 agcaggaaat tcacttaacg ctaatatatt accgtttatt cttagaggta ttaaattaat
     6421 tggcattgat tctgtatatg ttgatcataa tatccgtgaa gatatatgga gtaagttagc
     6481 aaatgaatgg aatataggaa attcacttct atacaatgaa attggatttg aagaattcta
     6541 taaaacgata gatcagcttt taaaaggaca acatttagga agaactattt taaaagtata
     6601 agtgtgagta ttcattaata catttactat taattcaatc attttgcatt tctctactaa
     6661 ccagtgttat aatttatata acgaaaatga ttaacaatta atgcaccaat ccccacacta
     6721 ttgcagtagt gtggggattt tttcgatgct ggcttataaa gtcgcctatt aattatttgt
     6781 tgcgtttact aagccaataa gtataatacg caacagcaca gccactgatg actggtgcta
     6841 tgatgtgaac gaaaatgatt agcaaagtaa tgcacctcct ctcttcgtca attgacgcct
     6901 gagagatagg cgactctatt attataccat ctgtgaatat acaagcatat ctacttccga
     6961 taagtcgtct tatgttatat aactttgttt tttatagtat caagacaaga agaaactcgt
     7021 ttcaaaaacc ctcatgattt ttagagaaaa actctcttat ttcatcgtct ttaataaaat
     7081 aatatgccat ctttccatct ttataaaaat caagtacctc atttttatat aaaagcctta
     7141 aatgatgtga agttgaagca acactcattt tcaatatcaa agaaatatca caaacacaca
     7201 actcatcttc tttaatcaaa gataatatga ttttcaattt cttctcatca caaatttttt
     7261 ctaaaatgtt aagtaatttc ttagatttat catcttctaa aaaacttaaa gcattgttaa
     7321 ctttatcctc atgtacacag atcacatcac aagtattttc ataactcata taaaatcaca
     7381 ccctaaatta aaatataaat cctaaaattg tttgaatagt gtcattttca ataataataa
     7441 ataaacctaa agctatataa ataatagcca taatccaacg actaaatttc tcaacaattt
     7501 ctccaattcc tggaatatta gctaattttt gtgcagtaaa caccaagaag aaaattaaaa
     7561 ttaaaaagac aaacagagta agtaataaat tagtaacact taatgtcaca aaatacggaa
     7621 caaataaacc aatattatcg gcgccacaac ttgctatcgt aacaattgca accgtaccaa
     7681 ctaatttaga caatcctttt tcattcaatt cttttttagc tctcttttct ccttcacaat
     7741 catcataaat agccacttta attccaagat aaattggtat taaacccaat aaacctaata
     7801 tccacttttc tggaacataa tttaatacaa aagctaaaaa caaactaact aatattaaaa
     7861 taatagaccc taaatattga ccaacataaa tatctctata ttcttttcta gtctttgctc
     7921 tagcaaaaaa tattaataaa attactaata aatcaactgc tgtagcaata taaagaacag
     7981 cagcagcaac aaccgtttga atcataatac acctcattca aaaatatttt tgaatgtatt
     8041 ataacttata tttttaaata atcataggtg
//
LOCUS       DANRDI010000114         8008 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.114, whole genome shotgun sequence.
ACCESSION   DANRDI010000114 DANRDI010000000
VERSION     DANRDI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.114"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1893
                     /locus_tag="PZ377_002011"
     CDS             <1..1893
                     /locus_tag="PZ377_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDM6358876.1"
                     /translation="EKLEQVAKEMGVDIKVETQGGVGAENVLTTQDIEEADGVIIAAD
                     KQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAPIYQSETNYHSKDRGKSKSGI
                     QMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSKGLVIPDDSFWKSIENIGSLA
                     FKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFYGSDAGAGFLGGIVAGFLAGY
                     IAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFLIGAPISNIFEALTTWLKSMQ
                     GANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEGNYAVMGMVAVAVCTPPIGLG
                     LATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAAQDPLRIIPANIIGAMIASVI
                     AAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSLVTMTTVLLLKRNTPVIAVDA
                     PAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVFDQQTMIITDHDMSRNEAIDM
                     LIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAIPHAKSDVVKQPIVAVMKNNH
                     GVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKALMNDETRQSLINANSTTEIY
                     NLLMKI"
     gene            1907..2845
                     /gene="manA"
                     /locus_tag="PZ377_002012"
     CDS             1907..2845
                     /gene="manA"
                     /locus_tag="PZ377_002012"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDM6358877.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(2957..5938)
                     /locus_tag="PZ377_002013"
     CDS             complement(2957..5938)
                     /locus_tag="PZ377_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDM6358878.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            6149..8008
                     /locus_tag="PZ377_002014"
     CDS             6149..8008
                     /locus_tag="PZ377_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDM6358879.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
BASE COUNT         2599 a         1452 c         1343 g         2614 t
ORIGIN      
        1 gaaaagcttg agcaggtagc aaaagaaatg ggtgtggata ttaaagtaga gacacagggc
       61 ggtgttggtg ctgaaaatgt tttaactacg caagacatcg aagaagctga cggtgtcata
      121 attgcagctg ataaacaagt tgatctgtcc cgatttgttg gtaaacggtt gattaatgaa
      181 aatgttcgcg aagggattca taatccgaga ggtctaattc aacgtatcat taatcaagat
      241 gcgcctattt atcaatctga aacaaattat cattcgaaag atcgcggtaa gtctaaaagt
      301 ggtattcaaa tggtgtatca acatttaatg aacggtgtat cgtttatggt tccttttatc
      361 gtagttggtg gactccttat cgccatcgcg ctgactctag gcggtgaaac gacatcaaaa
      421 ggattagtca tcccagatga ttcattttgg aaatccattg aaaacattgg tagtttagcc
      481 tttaaattta tggttccaat actagctgga tatatcgctg ttagtatcgc tgacaagccc
      541 ggtcttgttc ctggtatgat tggcggcgct atcgctgctg acggtagttt ttatggcagt
      601 gatgcaggcg caggattttt aggtggtatt gttgctggtt tcttagcagg ttatatcgca
      661 aagtggatta aagatattaa agttccaaaa gcaatggcac caattatgcc tatcattatt
      721 attcccatta tttcatcggt cgttgtagga ttgatattta tatttttaat cggtgcacca
      781 atttctaata tttttgaggc attaacaacc tggttaaaaa gtatgcaagg tgctaatatt
      841 attatacttg cgatgattat aggtgcgatg attgccttcg atatgggcgg tccagtaaac
      901 aaggtagcat ttttatttgg ctcagcactt attgctgaag gtaattacgc cgttatgggt
      961 atggtcgcag ttgctgtatg tacaccacca atcggtttag gtttagcaac gtttgttcaa
     1021 aagtataaat tcaatcattc cgaaagagaa atgggtaagg cttccttcac tatgggacta
     1081 tttggtatta ctgaaggtgc tattcctttc gcagcccaag atccattgcg cattatacct
     1141 gcaaacatca ttggtgcaat gattgcatca gtcattgcag ccattggtgg tgtcggcgat
     1201 agagtcgcac atggaggtcc aatcgtggct gtattaggtg gtattgatca tgtattatgg
     1261 tttatctttg cagtgatagt aggtagtctt gtcacaatga ctactgtact attattaaaa
     1321 cgcaatacac cagttatagc agtagatgca ccagcgcaac acacgcaact tcatgataca
     1381 gatattacac aacatgatac agaggttgac aatgtagata acaccgcgaa acattcacat
     1441 ctcaataaac cttctcatgt ttttgatcaa caaactatga taattactga tcacgacatg
     1501 tctcgaaacg aagcaattga tatgttgatt cataaactta aaatttgccg ttatgtagag
     1561 cacacatcgc atttaaaaaa tgcaatacta gaacgtgaaa tggggtctac gacagctatt
     1621 ggcatgaacg tggccattcc acatgcaaag tctgatgttg taaaacaacc gattgtagct
     1681 gttatgaaaa ataatcatgg tgttaaatgg gacagtctag atggtagtct acctcaattg
     1741 atattcttaa ttgccgtacc aaacaacagt caggatacac atttaaaaat acttcagcgc
     1801 ttatctaaag cgttaatgaa tgatgagaca cgccaatcac taattaatgc aaacagtact
     1861 acagaaattt ataacttatt aatgaaaata tagaggagtt ttaactatgg cattattttt
     1921 acaacccgtt tttaaagaca gaatttgggg cggtcatgca ttaaaagcat ttaactatga
     1981 tatccctaat gaaacaactg gtgaatgctg ggcaatttct gcacatccaa atggtcctaa
     2041 tacaattatc aatggtccat acaaggatat gaccctagat caactttggt cacaacatcg
     2101 tgaattattc gataatgatt cacgagatag tttccctctg ctaactaaag tattagatgc
     2161 caatgataaa ttatctgttc aagttcaccc agatgatgac tacgctttaa aacacgaagg
     2221 cgaactaggt aaaacagaat gttggtatat tttagatgca gccccaggtg ctgaaattat
     2281 atatggcgta catgcacata ataaacagac gttaatagac atgattgata atcatgaatt
     2341 tgacagtctt ttcaaacgta tacctgttaa gcccggtgac ttttattatg ttcctgctgg
     2401 cactgtccat gccattggtt cgggtatttt aattttagag acacaacaat cctccgatac
     2461 gacataccgt atttatgact atgatagacg agatcaaaat ggtcaattgc gtgatttgca
     2521 cttagagcaa agtaaagatg tgattgaatt aggcaatcat gaaccaaata ctactcctat
     2581 aacaactcac atcgacacac acactgtaac tcaatttgta gataatcaat attttgcagt
     2641 atataaatgg gatattcatg gagatttaaa attcaataaa ccacatgctt attgcttagt
     2701 tacaattatc gatggtactg gtactttaac agtaaacgat aagcactatg acatccaaaa
     2761 aggttctagc tttattttaa ctacggaaga cgaggatatt cactttgtcg gtaatattag
     2821 tgctatagta agtcatcctg gataatagca atgataacaa ccgtcccata aaagacagaa
     2881 attactatct cttctgtcta aaaacataaa aatccacaca ttaaggagct ataaccctaa
     2941 tgtgtggatt taatttttat tctgtaacgt tactttggtc aactttttca gatacgcgct
     3001 tcatcaatgg atctgttaca ggttttaaaa ttaaacctac aacgaagaat cctataccaa
     3061 ataacgttaa tataattaat tttgtaatta ggatttccgg aacaataccg cctactgttt
     3121 cacgtaatga atcaattgca tacgtaaatg gtaagtatgg cgaaatgttt tggaaaaatt
     3181 gtggcgtagt ttgaattggg aatgttcccc caccacctgc aatttgtaat acaagcaata
     3241 cgattgcaat ggctttacct gggttaccaa gtaatgatac gcacgtatat acgattgagt
     3301 tgaaaataat cgaacagaaa atcgttataa gtacaaataa tacaggtgac tcaactcctg
     3361 cttttaggat taacaaatct ccaaccgata caatgagtgc ttgcaacata ccaagcatta
     3421 taaagaatcc tgccttacct aagaacactt gtcttgtcgt taagactgac tctagactct
     3481 tatgtttatt atcaaccgtt aataaactta ccatcaagag tgcacctacc catactgaca
     3541 gtgcagtata gaatggtgtc atacctgaac cataatcctt aactgggaaa acatcaacag
     3601 tcttcttatt aataggattt gcaataatac ccgcttgctt tttcaaatca ttcttaagtg
     3661 tgtcgattaa tttgtcgact gcatcatctt tgtctaattt cttgaaaatt ttattcgctt
     3721 ttttcaaatc tttttcaaca tcaggcaatt tattttgaga aaaatcagct aaatctgata
     3781 aagccttttt cgcttgtggc tgatttttat ttagtaatcc taccgcttga tcataaccat
     3841 ttaataacgt tggtaaattt ttattcacac ttgctgtcgc attagttaat cgctgttcta
     3901 actgtggtaa gtcattgcgc acaaagttag cagctttagc tacatcactt ttcactttcg
     3961 agaagtttgc ttgagcattt ttaacagcac taatgtattg ttgttcaata cttggtaata
     4021 ctgtttgtaa gcgatcaata cgtgttttac cattttttaa aatagcttgt ccactgttaa
     4081 tgacttgatc aattgtattt aatttagact gaacattgga taatgcagtt tgccctttgt
     4141 tcaattcatc cataatacgt tgtgatattg atactaaaga gctgttaaga tcttttttaa
     4201 cataatctct aaatgatgat aatgatgaat ctagttttga aagttgatct agtaatttag
     4261 tagcttcagc agttcctgaa ctaccattct ttaatgcatt gcttaattga ttaactaacc
     4321 ttaatgattc attaattcga ttattagctg cttttacctt atcaatttct tttgataaat
     4381 caacattttc attcttctca atctctttta atgtatcgat aaattgttga ttatattgcg
     4441 ttgtgtattc taaaccggac ttaacattat ctaaagactc tctaaaatca gaaggcttgt
     4501 ccgatgctaa aatcccatac gaaatattac gtaatgcctg actatctttt tgtgcggctt
     4561 tcgcttgggt atctaaatta tttgataatg ataataaaga gcccgttaat gccgcttcca
     4621 ttgatttaac atctttatct gtaatgtttt gtgtgttctc gttagctgtg ctgacttgtg
     4681 ttgttgttat atctacaaaa ctttgaccat cttttttccc gcttgtactt tgtggtgtag
     4741 tcgatacagg cgcattatta ctatttggtg cggtattttg cgtaacatta ttatttggtg
     4801 cagtatcgcc acttggtgca cttggtggcg ttgatgctac accattacca gctgcaggtg
     4861 cactatttga tgtttcgttg ttcgccgcgc tattttgagg aatctgttgc tggtttcttc
     4921 gatttaaatc ttgcgcgcgt cgaattgcat cttgataatc accgactcta cttctaactt
     4981 gtgtgacgaa gcctttggca tcatttaact gtccattagc ttgatcaata ccttgactcg
     5041 caatgttaag tccttgatta attttaggga actgcgctgg cacgtcatca gctgcaaaat
     5101 ttaacgcttt ttcaattttc ggcatataat tatttaaagc taatatcaac ttagcctttt
     5161 cattaagttg cggaatagca ccattgactt cgtttaattt tttctgagca tctaaaatat
     5221 cacctttata atttcctagt tttctaaaat cattggcata tttatctaaa tccgcttggt
     5281 ggttattcaa atatacaatt ttattcgcaa agtcattaat cttaggtaaa gctttatctg
     5341 ctgaatatac cgcatttttt atcttgttaa ttgtcggcac attttcttca atagttaaac
     5401 ctgctttgtt agcttcttct aataatgctc gagttactgt tttattaaat tgttcattcg
     5461 ctttttcaac gacaactgac gaaccagtat ctgttagctt agacgcaaca gcgttaatct
     5521 tctgattcac cttaaattct acatctgctt tttgaggctg cttacgtagt gtccctgtaa
     5581 tttcatgtgt aaacttagat gggatgtaaa tacctgcaaa atatttaccc atttttatct
     5641 catgatcagc tttctctcta cttacaaact gccaatcaaa acttttattt ttcttgagtg
     5701 tattaaccat cgtattaccg acattaactt ttttccctct gattgtgtcg cctttatctt
     5761 cattaacgac tgcgaccttg atgtgtcccg tgttgccata tggatcccac attgcccata
     5821 agttaaacca agcgtagaac gatggcaaaa tagctaagcc tgctaagata atccacacag
     5881 ctggcgtctt agctactttc ttcagatcca ttttaaataa tttaaatgcg ttcttcattg
     5941 tcacactcct atgtaggaat tattcatatt ttttatatat tttttgtaaa ttaatttatt
     6001 tttgcgttgt gaattagtat aatcaattta ctggaagata tttagtcgat tgatacctat
     6061 caactatttt cagcatacga taaattataa caaatcatag tttattatca cacttaatta
     6121 ttatattttt caagggagaa tacgaaatat gcctaaaaat aaaattttaa tttatttgct
     6181 atcaactacg ctcgtattac ctactttagt ttcacctacc gcttatgctg atacacctca
     6241 aaaagatact acagctaaga caacatctca tgattcaaaa aaatctaatg acgatgaaac
     6301 ttctaaggat actacaagta aagatattga taaagcagac aaaaataata caagtaacca
     6361 agacaataac gacaaaaaat ttaaaactat agacgacagc acttcagact ctaacaatat
     6421 cattgatttt atttataaga atttaccaca aaccaatata aaccaattgc taaccaaaaa
     6481 taaatacgat gataattact cattaacaac tttaatccaa aacttattca atttaaattc
     6541 ggatatttct gattacgaac aacctcgtaa tggcgaaaag tcaacaaatg attcgaataa
     6601 aaacagtgac aatagcatca aaaatgacac tgatacgcaa tcatctaaac aagataaagc
     6661 agacaatcaa aaagcaccta aatcaaacaa tacaaaacca agtacatcta ataagcaacc
     6721 aaattcgcca aagccaacac aacctaatca atcaaatagt caaccagcaa gtgacgataa
     6781 agcaaatcaa aaatcttcat cgaaagataa tcaatcaatg tcagattcgg ctttagactc
     6841 tattttggat caatacagtg aagatgcaaa gaaagcacaa aaagattatg catctcaatc
     6901 taaaaaagac aaaaatgaaa aatctaatac aaagaatcca cagttaccaa cacaagatga
     6961 attgaaacat aaatctaaac ctgctcaatc attcaataac gatgttaatc aaaaggatac
     7021 acgtgcaaca tcattattcg aaacagatcc tagtatatct aacaatgatg atagcggaca
     7081 atttaacgtt gttgactcaa aagatacacg tcaatttgtc aaatcaattg ctaaagatgc
     7141 acatcgcatt ggtcaagata acgatattta tgcgtctgtc atgattgccc aagcaatctt
     7201 agaatctgac tcaggtcgta gtgctttagc taagtcacca aaccataatt tattcggtat
     7261 caaaggtgct tttgaaggga attctgttcc ttttaacaca ttagaagctg atggtaatca
     7321 attgtatagt attaatgctg gattccgaaa atatccaagc acgaaagaat cactaaaaga
     7381 ttactctgac cttattaaaa atggtattga tggcaatcga acaatttata aaccaacatg
     7441 gaaatcggaa gccgattctt ataaagatgc aacatcacac ttatctaaaa catatgctac
     7501 agatccaaac tatgctaaga aattaaacag tattattaaa cactatcaat taactcagtt
     7561 tgacgatgaa cgcatgccag atttagataa atatgaacgt tctatcaagg attatgatga
     7621 ttcatcagat gaattcaaac ctttccgtga ggtatctgat agtatgccat atccacatgg
     7681 ccaatgtact tggtacgtat ataaccgtat gaaacaattt ggtacatcta tctcaggtga
     7741 tttaggtgat gcacataatt ggaataatcg agctcaatac cgtgattatc aagtaagtca
     7801 tacaccaaaa cgtcatgctg ctgttgtatt tgaggctgga caatttggtg cagatcaaca
     7861 ttacggtcat gtagcatttg ttgaaaaagt taacagtgat ggttctatcg ttatttcaga
     7921 atccaatgtt aaaggattag gtatcatttc tcatagaact atcaacgcag ctgccgctga
     7981 agaattatca tatattacag gtaaataa
//
LOCUS       DANRDI010000115         7983 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.115, whole genome shotgun sequence.
ACCESSION   DANRDI010000115 DANRDI010000000
VERSION     DANRDI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.115"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(387..1304)
                     /locus_tag="PZ377_002015"
     CDS             complement(387..1304)
                     /locus_tag="PZ377_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDM6358880.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            1395..1496
                     /locus_tag="PZ377_002016"
     CDS             1395..1496
                     /locus_tag="PZ377_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358881.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            complement(1574..3079)
                     /locus_tag="PZ377_002017"
     CDS             complement(1574..3079)
                     /locus_tag="PZ377_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDM6358882.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(3420..3920)
                     /gene="queF"
                     /locus_tag="PZ377_002018"
     CDS             complement(3420..3920)
                     /gene="queF"
                     /locus_tag="PZ377_002018"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDM6358883.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(3940..4806)
                     /locus_tag="PZ377_002019"
     CDS             complement(3940..4806)
                     /locus_tag="PZ377_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDM6358884.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            5607..6005
                     /gene="nrdI"
                     /locus_tag="PZ377_002020"
     CDS             5607..6005
                     /gene="nrdI"
                     /locus_tag="PZ377_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDM6358885.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            5968..>7983
                     /gene="nrdE"
                     /locus_tag="PZ377_002021"
     CDS             5968..>7983
                     /gene="nrdE"
                     /locus_tag="PZ377_002021"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDM6358886.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLY"
BASE COUNT         2839 a         1223 c         1306 g         2615 t
ORIGIN      
        1 taactcatgc attcatattt ttaagtacac attagctgtg actaatgata aagaatcgct
       61 acataatcaa tcattagtcg ttctttatca tttccgtccc gctctcaata aatgttagtt
      121 tatcttatta ttataaatcg gatgaatgtg ttaatctatg gcagattaca cgtcatccga
      181 ttttttatag aatttgaaaa agacgcataa accactatga tttaaaatac aacatcaatc
      241 attttagtgg catgcgccaa aattatatgt ctgtttttga aacagggtaa tagcttaaag
      301 ctaataaaaa cgaatataag gtgcgttgaa tcttatgatt acactccaaa cctaatataa
      361 tatcgggtta agatcattcc ggatgcttac aaatcattga cagtaagtaa ctgaatggca
      421 tttggtataa cctcaatatc aataggtgtt tctaatgaaa tttcgccatc aatatcaact
      481 ttcattgctg gatctgttgt aagtgaaatc tttttaccag gtatatgctc aataccttga
      541 gtaatttcat tccaattcat gctatcacgc tttttaaaaa tatcatttaa aatactgaaa
      601 ctttgttcat taaaaatgaa agtgttcagt tcaccatctt gaggagacaa atcagtcaat
      661 ggtatacgac taccaccaat gaatggacca tttgctgtta gtatcatggt cgtttcgcca
      721 gaatatgtct tatcatctat tgataattga taattaaatt gtgttggatt tagcagtgtt
      781 ttgacagttg atccaatata actcaattta ccaaatatat cttttgaacc atcttgtacg
      841 ttttcagcgt tttgaacaat gagacctaag ccaacaaagt tgagtgcata ttgattattt
      901 attttaatta catcgtatgt accaacttgt gcagaaatca tttgttcact agcttgttta
      961 tgattaggtg ctatatttag cgtttttgta aaatcattaa aagtaccgcc tggtaaaatg
     1021 ccaataggga gttgaaggtc atgtgtcata acaccgttta taagttcgtt aaccgtgcca
     1081 tcaccgccaa gaataaataa tatatctaca tcttttgcat agtttttagt tttgatttct
     1141 tggcaatatt taataatgtc accttcgttt tcactcaatt gaatagaaag atgcttacaa
     1201 attgaactta atgctgttgt aacttcccca ataccttgat taatattttt taatccactg
     1261 tgttcatggt aaaagaggac accatgtgta tatttatttt ccatagttta gcctactttc
     1321 taaaaattgg ttcattaaat atatataccc acttttaatt gttaatacca aaaatatgtt
     1381 tttaaataga gaaaatggta ataaatgaaa ttgatttcta tagagtggga cgagaaaata
     1441 tagttatagc tgtctataat gagcatatta agtttttatt tatactgata tcttgaattt
     1501 aattaataga aacctataaa aaaacagtaa gccatttaaa tgacttactg ttttttgaat
     1561 taggccaaca atattaacgt atacctttca tcgctttgat gattaaaggt gagattgcta
     1621 atacaattgt tgtaacaata attgcaacaa cacctaggaa aataaagtaa tttgtttgac
     1681 ctagtggttc tattaactta actaaagtac cattgattgc ttgtgcagaa gcgttagtta
     1741 agtaccaaat actcatcatt tgggcattaa atgctttagg tgctaactta acagcagcac
     1801 tattacccgt tggtgataag catagctcac cgataacaca aataatgtac gataaaataa
     1861 cccagttaac tgaaaagttt gatgaacctg atgcataacc tacaatacca attagtatgt
     1921 atgacgcacc tgctaagaac gtaccaattg caaattttac tggcaggcta ggttgtttag
     1981 ttccaagctt ttgccataaa agtgaaataa ttggagctag taataaaata aataatgggt
     2041 taattgattg gaagatcgct tcaccaaagt ttgttttcca accaaataag tttaatttca
     2101 tatctgaatg ttcaattcca tatatgttta atacattaga cccttgttct tgaatagccc
     2161 agaacaccat tccaagaata aataatggaa taaatgcttt aacacgagaa cgttcagtat
     2221 cagtgacatc tttacttcta ataattaaag tgaagtaaat gattggtaat gcaataccta
     2281 atactaaaac agtattacta actaagttaa atgataatga gttagttaat gcaccaataa
     2341 cgataattaa tacaattgct aaaacaacac ttccgataat aagaccatac tttttctttt
     2401 cagctggtgt caatgggtta gtaggtttca taccaacgct acctaagttt ttgcggttga
     2461 aaagtacata ccatactaaa cctaatgcca taccaactgc tgcaatcaag aatccgccgt
     2521 ggaagttttt aacattaaca aagtgttgca aaataatagg tgataataat gcacccatat
     2581 taactgacat atagaaaata acaaaacctg catccatacg tctatcattt tcaggatata
     2641 aacggccaac gatatttgaa atgtttggct tcattaaacc tgaaccaata atgatgaaga
     2701 acattgatgt gaataagccg attaatgcaa atggtaagct taaacaaata tgtccgataa
     2761 taataaagac tgcacctaat aaagtagcgc ctctagtgcc tgtaattctg tcagcaatcc
     2821 atccgcctgg tattgatgtc atatagatta atgaaccata aactgacata attgacatag
     2881 ctgttgtttt atcaattcca aggccattat ctgttacggc aaagtacatg tagaaaatga
     2941 gtagggcacg catgccataa taactaaacc tttcccagaa ctctacaaag aagagtacgc
     3001 ctagtcctcg aggatgcccg aaaaatcctg tttgaggtat gtcttgaatt tgatttccat
     3061 gggagttttg ttgtgtcatg tatacatccc atcctttctt cccctaatac aatagtttaa
     3121 gtaaatttga ttgtgcctta tcaactaaat attccaacaa atcacaaaaa aataaaaata
     3181 ttgagaatat tctgttatta agcgcgcaca attaattaga gataatataa aaatatactt
     3241 attgacgtta aagtgcaata ggtttctata aaaatgttgt ttaattcgtg tactaatatt
     3301 cttttggtgt actttggtat aaaatgttat tttaatgtaa cctattatgt atgctaaatg
     3361 aaaaacacac caataagtta ggaaaatcaa cttattggtg tgtttattaa atgatacatt
     3421 taacgattat ctattttttc gggatataaa tcatgattca tcaaacgatg ctcagccatt
     3481 ttttcatatt tagaatttgg acgtccatag tttgtataag gatcaataga aattccacca
     3541 cgtggtgtga acttgcccca gacttcaata taatgtgggt ccataagctc tatcaaatca
     3601 ttcataataa tattcataca atcttcgtga aaatcaccgt gatttctgaa actaaataag
     3661 tataatttca aagattttga ttcaaccatt ttaacatttg gaatatatga aatatagata
     3721 gttgcaaaat ctggttgccc agtaattgga cataatgatg taaattctgg acagttgaat
     3781 tttacgaaat agtcacgacc ttgatgctta ttatcaaacg attctaatac atcaggacga
     3841 tagtcaaaat tgtaagtatt gtcttgattt cctaataaag ttatatcttg taattcatct
     3901 tgttgacggc catgtgccat ataaagcgct cctttaaatt tattttttta ttattttggc
     3961 gtctcggcgt gctttttcaa acatgtaata acttgcaccg ataataacga cgtaacctaa
     4021 tgttgcatag aaatctggag attctccgaa tagaataaat ccaagtattg ctgtgaaaat
     4081 tatagatgca tacgtaaaaa tagaaatatc ttttgctgct gcaaaactat atgctaaagt
     4141 aacaccaatt tgacccacag cggcagctaa gccagcccct aatagataaa gtatttgcat
     4201 ctgactcatt ggttcataag tatatgcagt gaaaggtatt aaaacgatga cagaaaataa
     4261 ggagaagtaa aatactatag tatatggtgc ttctcttgta ctaagtgctc gaacacatgt
     4321 atatgctgat gctgcaaaaa tacctgagaa taagccagct aatgatggaa tcatagatga
     4381 tgaaaattca ggtttcacta ttaaaagcat acctaaaata gcaattatca ttgctgtaat
     4441 ttgatacttc cttacctttt catgtaagaa aacaatgctt aataaaatcg tccagaaagg
     4501 attgagtttc attaatgaat cggcatcact aagtaccata tgatcaatgg cataaatatt
     4561 taacaataca ccaataagtc caagtgttga tcgtgttatt aataagggtt gacttgaaag
     4621 tctgccaaac attggctgat ggtatttata tataaaaaat aatggaataa acattgctac
     4681 taagtttcgt gctaatgatt tttgaaaaac aggaaggtca cctgcaagtc tgaaaaacac
     4741 tgacataaaa ctgaaaccaa tagccgaaat taaaatggca atgatacctt ttactttagg
     4801 attcaatttt atcgcctctt ttatataaaa ttaacgtatt tatattagca taaaacaaca
     4861 tgttgtgcat aaatagttga aatttactat aaaaagacta taatagactg tagcgaacaa
     4921 acgttctgtg tttatttgtc ggaataatag ggcattacac ttttatgaat gtttgtgtta
     4981 ttacataaaa caaatatcaa ttcagtatca agctaataag ctttttcttg atttctgttg
     5041 atacaattga gattgacaca gatttaaaaa aatcaagtga tatctactaa aaaatttttt
     5101 taaatttgtt caagtttttc taatttagta ttggtgccta gttggaacgt tttacgaaca
     5161 ttcgattaga aaatggcact ttaaatcata gtgtgtctta tgtataatga aacacataat
     5221 atagtgttgg tgaaacgaaa aagacacaat atcttgtgtt ttgtatgcaa atgctttatt
     5281 tatgaagaaa ttacatttaa aagtaattta acacagaaat ttaatagtta ttatcaatta
     5341 atagtcatat ttttagaaaa tgtactgagc aaatggaaga tatccaatga tgtaaacact
     5401 acatatagtg atttttatac attcaaccca tataagctac tattttctca aatataaatc
     5461 tatgcaattg gtttacattt gagaaaataa gtagcttcat tatagttaat acaatgctga
     5521 gataaccata gtaaccatgt tgttaaagca ttttttaatt ggaatgacta ctttatttaa
     5581 aagggttgaa gaaagaaggt gatccaatga aaataatata tttttcattt actggaaatg
     5641 tccgtcgttt tattaagaga acagaacttg aaaatacgct tgagattaca gcagaaaatt
     5701 gtatggaacc agttcatgaa ccgtttatta tcgttactgg cactattgga tttggagaag
     5761 taccagaacc cgttcaatct tttttagaag ttaatcatca atacatcaga ggtgtggcag
     5821 ctagcggtaa tcgaaattgg ggactaaatt tcgcaaaagc gggtcgcacg atatcagaag
     5881 agtataatgt ccctttatta atgaagtttg agttacatgg aaaaaacaaa gacgttattg
     5941 aatttaagaa caaggtgggt aattttaatg aaaaccatgg aagagaaaaa gtacaatcat
     6001 attgaattaa ataatgaggt cactaaacga agagaagatg gattctttag tttagaaaaa
     6061 gaccaagaag ctttagtagc ttatttagaa gaagtaaaag acaaaacaat cttcttcgac
     6121 actgaaatcg agcgtttacg ttatttagta gacaacgatt tttatttcaa tgtgtttgat
     6181 atttatagtg aagcggatct aattgaaatc actgattatg caaaatcaat cccgtttaat
     6241 tttgcaagtt atatgtcagc tagtaaattt ttcaaagatt acgctttgaa aacaaatgat
     6301 aaaagtcaat acttagaaga ctataatcaa cacgttgcca ttgttgcttt atacctagca
     6361 aatggtaata aagcacaagc taaacaattt atttctgcta tggttgaaca aagatatcaa
     6421 ccagcgacac caacattttt aaacgcaggc cgtgcgcgtc gtggtgagct agtgtcatgt
     6481 ttcttattag aagtggatga cagcttaaat tcaattaact ttattgattc aactgcaaaa
     6541 caattaagta aaattggggg cggcgttgca attaacttat ctaaattgcg tgcacgtggt
     6601 gaagcaatta aaggaattaa aggcgtagcg aaaggcgttt tacctattgc taagtcactt
     6661 gaaggtggct ttagctatgc agatcaactt ggtcaacgcc ctggtgctgg tgctgtgtac
     6721 ttaaatatct tccattatga tgtagaagaa tttttagata ctaaaaaagt aaatgcggat
     6781 gaagatttac gtttatctac aatatcaact ggtttaattg ttccatctaa attcttcgat
     6841 ttagctaaag aaggtaagga cttttatatg tttgcacctc atacagttaa agaagaatat
     6901 ggtgtgacat tagacgatat cgatttagaa aaatattatg atgacatggt tgcaaaccca
     6961 aatgttgaga aaaagaaaaa gaatgcgcgt gaaatgttga atttaattgc gcaaacacaa
     7021 ttacaatcag gttatccata tttaatgttt aaagataatg ctaacagagt gcatccgaat
     7081 tcaaacattg gacaaattaa aatgagtaac ttatgtacgg aaattttcca actacaagaa
     7141 acttcaatta ttaatgacta tggtattgaa gacgaaatta aacgtgatat ttcttgtaac
     7201 ttgggctcat taaatattgt taatgtaatg gaaagcggaa aattcagaga ttcagttcac
     7261 tctggtatgg acgcattaac tgttgtgagt gatgtagcaa atattcaaaa tgcaccagga
     7321 gttagaaaag ctaacagtga attacattca gttggtcttg gtgtgatgaa tttacacggt
     7381 tacctagcaa aaaataaaat tggttatgag tcagaagaag caaaagattt tgcaaatatc
     7441 ttctttatga tgatgaattt ctactcaatc gaacgttcaa tggaaatcgc taaagagcgt
     7501 ggtatcaaat atcaagactt tgaaaagtct gattatgcta atggcaaata tttcgagttc
     7561 tatacaactc aagaatttga acctcaattc gaaaaagtac gtgaattatt cgatggtatg
     7621 gctattccta cttctgagga ttggaagaaa ctacaacaag atgttgaaca atatggttta
     7681 tatcatgcat atagattagc aattgctcca acacaaagta tttcttatgt tcaaaatgca
     7741 acaagttctg taatgccaat cgttgaccaa attgaacgtc gtacttatgg taatgcggaa
     7801 acattttacc ctatgccatt cttatcacca caaacaatgt ggtactacaa atcagcattc
     7861 aatactgatc agatgaaatt aatcgattta attgcgacaa ttcaaacgca tattgaccaa
     7921 ggtatctcaa cgatccttta tgttaattct gaaatttcta cacgtgagtt agcaagatta
     7981 tat
//
LOCUS       DANRDI010000116         7891 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.116, whole genome shotgun sequence.
ACCESSION   DANRDI010000116 DANRDI010000000
VERSION     DANRDI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.116"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..431)
                     /locus_tag="PZ377_002022"
     CDS             complement(<1..431)
                     /locus_tag="PZ377_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HDM6358887.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDK"
     gene            complement(418..624)
                     /locus_tag="PZ377_002023"
     CDS             complement(418..624)
                     /locus_tag="PZ377_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358888.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(849..1193)
                     /locus_tag="PZ377_002024"
     CDS             complement(849..1193)
                     /locus_tag="PZ377_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDM6358889.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            complement(1864..4236)
                     /locus_tag="PZ377_002025"
     CDS             complement(1864..4236)
                     /locus_tag="PZ377_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDM6358890.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(4328..4576)
                     /locus_tag="PZ377_002026"
     CDS             complement(4328..4576)
                     /locus_tag="PZ377_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HDM6358891.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(4577..4960)
                     /locus_tag="PZ377_002027"
     CDS             complement(4577..4960)
                     /locus_tag="PZ377_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDM6358892.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(4962..5165)
                     /locus_tag="PZ377_002028"
     CDS             complement(4962..5165)
                     /locus_tag="PZ377_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDM6358893.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(5132..5305)
                     /locus_tag="PZ377_002029"
     CDS             complement(5132..5305)
                     /locus_tag="PZ377_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDM6358894.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(5317..5589)
                     /locus_tag="PZ377_002030"
     CDS             complement(5317..5589)
                     /locus_tag="PZ377_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDM6358895.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(5582..5845)
                     /locus_tag="PZ377_002031"
     CDS             complement(5582..5845)
                     /locus_tag="PZ377_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358896.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            6038..6370
                     /locus_tag="PZ377_002032"
     CDS             6038..6370
                     /locus_tag="PZ377_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6358897.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            6382..6840
                     /locus_tag="PZ377_002033"
     CDS             6382..6840
                     /locus_tag="PZ377_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDM6358898.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            6857..7585
                     /gene="seq"
                     /locus_tag="PZ377_002034"
     CDS             6857..7585
                     /gene="seq"
                     /locus_tag="PZ377_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HDM6358899.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
     gene            7609..>7891
                     /locus_tag="PZ377_002035"
     CDS             7609..>7891
                     /locus_tag="PZ377_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDM6358900.1"
                     /translation="MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDL
                     KDVKDNDTPIANQLQFSNVSYDLISESKDFNKFSNFKGKKLDVFGISYNG"
BASE COUNT         2523 a         1354 c         1033 g         2981 t
ORIGIN      
        1 aacttatctt taatgtatgt tagtgatggt atagattcaa catgttcatc atcagtaatg
       61 gtaatcacct cacgtagtga ttgaggtagc tcacaattca ccaataactc tttaaatact
      121 tctttacgtg cagttggtaa ctcttgaata ttaaagttat tctcactact aactaattgt
      181 tttgttaagg tacctaccca tgtattactt gattgattta cttcattcat atttaacact
      241 cctaataatt ttttgttaat ttgctctggt aataaaactt ctgtttcagt aatacttaat
      301 tgctttaacc cctgttcaat catacttaca tctttaccta tatccttttg atatagattg
      361 gcatatcctt taatatccat atcatcaaag ttagatagtt gatacccaaa ttgttcttta
      421 aactctttca taatcttcat ctcctaattt gtttttagta tgaacaacta aaccatatat
      481 acgttcgttg tttttatctt cttcatagaa cgaaatgtta tgaagaaagt atttgtctaa
      541 taattcgtga tacttattta atagttcttg cttatctcta tacttaaaca cacgttttac
      601 agattcattt gtatgatcta gcacgtcttg cctctctcct tttaatctga tagatataca
      661 ttttaataag ttcaaaatca gcacgctttt ttgtatagcg ccttacaacg cgatttatat
      721 atttcgtatt cattaattga acgttcacag gtgtatgttt gattatttga tatgcgattc
      781 ttttatgatt aataaatata cacactccca tatctatact tgatatttta aatgcttatc
      841 tgcatgtttt aatttactaa actaaatgtt actggtagcc aatgatctat ttttatattt
      901 ttattcttta caattggtag ccttagacct ttaccatcaa taagatagta cattggcgga
      961 caaatttgca tttcaattag cccgtcgcgt tttaaatctg ccacaatgtt aaacgcttct
     1021 tcattccaac ccatccaaaa tataatattc ttatggttgc cactggtata tgcgccgtta
     1081 cctttgtatt taaaaccttg ttcttcaaat acattctcaa tctctacaaa tgaagtgctg
     1141 tcattgttct ttatatattc taagatgatt tgtttcattc tatttctatt catgttactc
     1201 tagtcctcca atctatccag ttactaaagc aaccacgtta gatactaaaa accactgaat
     1261 ttatgttttt caataaccac tgtaaacaca gtgatgtcaa tacatacagg ctgttggtta
     1321 ctgagttact gaaaattcac ggtattttat ataatctttt attaccttaa ttctctatta
     1381 tacatttcta ctaaaaagaa ataatttata gtaactcagt aactttttgt caaaaagact
     1441 aatatgaaag taaaaactgt ggttactgaa ttctagaatt ttagtatctt ttagtgactt
     1501 aaacgttttt tagtaacttt agtatcttag gcaattttgt ataactgcaa gtctcttccg
     1561 attatgtgca actgaaattt acagctatgc aactcgtttt aaattttcca gttgcaattt
     1621 ttttcttact gtcacaaggg gttaatccac ttttgcaact atgcaaccga aatcacacaa
     1681 attagtttat ataatttttg atactatttc ttatttatat tttggttatt tacttttcta
     1741 ctaataagtt atagattttg agttgctcag ttgcataagt gccctaaacc cttgatataa
     1801 ctgtgattat aggtgcaacc cgttttaaaa ttttcggttg cattcggttg cattcggttg
     1861 caactatatt gcttttagct tcaccttttc atacgattta tagttttttc cactttcaat
     1921 aggttcaatg taatttctat tgtcattaaa gttgtatatc ctcttagcaa gttcttcatt
     1981 gtcatatctt cgctgtgcat cagttttcca ataattctct aaataatgtt caaattgttt
     2041 ataaaacttg tttgaactca atgcattata gccattttca tcacaatatt ctttataaaa
     2101 tgcgtataca atatatttcg gcacttttct aatagtccat tgatcaaaca tatttacttt
     2161 aaacccataa actggatcgt tatcttcttt aaatacttca agcatttttt tagatgcatc
     2221 aggaatgtca aaggtttcaa aatctaaatt aattgcttta taaagcacat actctagcac
     2281 ttgctgattt tttatatagt cttctttgat tttaaaattc tctttaatgc cattaaaatt
     2341 ggcattaaac ggtactatca ataaccttct taaagtcccg cctgttttgt ctttaaattt
     2401 aggcattcca ttcgttgatt gaataactgt acacttaaaa gtcgctctat ataagggttt
     2461 atttttaaac tcaaccaaca ctggatcgcc agtaactaca cttttaaagt ttgaagaatc
     2521 atcgacatac acaccaactg gtacatcgtc tccaattact gctgtcttgc cctctaacac
     2581 actcaattta aaacgttcat caaactcatt cacttttaag ctagcaatat tgctataacc
     2641 tattacatta gacaataatt cttgaaacgt ccctttacca ttatttccat caccaacaaa
     2701 gaaaatagcc tttttacgag tgtagttacc attcattgag tcattaatta cttgccacaa
     2761 taatttaaca acctgattat cattacaagc aatctcttct atccaccgat ctatattcca
     2821 gccatttatt tcaggtacta tatcttgcct tacatacgat gtatctattt tactggtaaa
     2881 tatataatca ggtgtaaatg attctagttg cttcgtttta cggttaaata caccattttt
     2941 aactggtatt aagtatggtg agttagtttt ctcttttata tctaccatat tggttaaatg
     3001 ataaataact tcatcagctt tgttgctatt atgtttgggc tctaaatagg aaatcactcg
     3061 ttttataatt gtagtgttct gtgtatatat cccttcatca aattgataca tagctaactt
     3121 agtattttct tcatcatcaa aaagtataaa tgttaaatgc tcattcaaaa tatatgcaca
     3181 ttttataggg ctaattgttg taggctttcg ccctcgtttt ccttcttcaa tccaattttc
     3241 ttccattttt gttctttctt catgccccaa ttgattcaac aactgtttaa catctttact
     3301 cccctgtggt ataacattaa attcatctat cgatttagta taaattgtct tttgattctt
     3361 tatagcttta tctaactcaa gctgtcccca agtcgtatta cctcgtttac tttcccattt
     3421 atccgtaaga ttattgtagt ttaaaaatat acgctccatt tgctgtttgt ttttacctgt
     3481 gtaaaacgct aagtagtgta ataagctttg tactgcttcg cttgaactct caaaatatga
     3541 ttcatatgtg ccttgtaata gatctttaat tttatctttt tgtttagatt tcatcataat
     3601 gtttataata tcttcatcag ataattgtat attggtatta gattcctctc tcacaacctc
     3661 atttgctggc aaattctctt taaaatattc atcaactaga gtatttaata cttcttgatt
     3721 atcacaaata tcactttgac caattgtgca tcctgtaaca gtcataaacc ttgctgaatc
     3781 atataactct atgtctaaat ctgttctttt ctttttacgg ttatctggta gtttaccttt
     3841 aaagaaacaa tgtaatcctg taccacttgg agacttttca caatatgtga gttgcatcat
     3901 ttttagtgct aattcagaat tgatttgtcc ttttttatca attgcattat caatatctag
     3961 gcaaacaaaa ttattaccat cacttaatac aaaacctata ccgctatatt gctcatcaac
     4021 ttcatattca atacttacac tttcaaagtc acaccatgtt tttttattgg ttgtactagc
     4081 tctgtaacca ttaatactat atggtacttt tccaaaattt tgttgcttct cgttccattc
     4141 agctcgccat aatacccaat tgggcactaa ttttaattct tgcggtattt ctaattcttt
     4201 aactccaata attctatctt ttttcttaat tgccacagaa tgcctccttt ctaatctgat
     4261 ttctctgtat tttcaatgca actaatgtta aaatattaat ggtatgattt tataaatttt
     4321 tattttttta tgcgttatct tggctttggt cggcgaaaga tgacgctttt tctaatgcat
     4381 caaacatttg atcattgtga atacgtctct catgatatga aaatccttct ttaatagcat
     4441 cgtctttatt taaggtatga tttggctcat gtagatataa atcttcaaaa tgcgagccat
     4501 gattatcctt taaatcctca aacttttctt tcaatatctt taaatcacac attaaatttt
     4561 taatttccca attcatttat aattcctcca cttcaatatt tcccacaatg taatctaatg
     4621 cccactctaa cattccaatc acgtgtcctt tgcgatctgt tgtatgttta tgttcacctt
     4681 tttcgtctat gacactataa cggtaaacgt gttgtgtttc tttcatgaca tcacttagcg
     4741 tcattgttac ttcatcaaga attaaaaatg cttcatcttc aaaatctaat tcagcaagaa
     4801 tattgaacaa tccattatga actaacttta aagcatattc atataatgct ttatcctgat
     4861 aatgatagtc cttatcggat tgaaacttat cttcctcgta taatactact tctaatattt
     4921 taaccacttt tgataattga tatttttgtt tgatttccat cttaactacc tacttttttc
     4981 ttatttttaa tttctgtaat ttttgataaa tccatttcta aacacgcaac ttgaacatct
     5041 ttactaacat caggaaagta ctgttcaaat acttttggcg gtatatttaa cattaaatta
     5101 tgttgagtat ctttaatgtt gtaccagcca actactgttt ttgtaataat aacttgtttt
     5161 cgcacgttgt aatctctcct gttaaattaa atccataagt taccatcatg ccgtacacac
     5221 taaaagcgac atacatgtta gatattgcta gtaataatat tgttaacaat gaaactaagc
     5281 agatataagt taagtacatt ttcattgcct tgcctcctac atccattttt tatgacgcgc
     5341 cttcatgtac tcctcgaatc gcggaatact gataacaatc attgttgatg ataacgagta
     5401 atataaatca tcaacacctt tagaatcttt ttcccactct tttaaaatgc gattcaccga
     5461 actatatgaa attcccaaaa tactagctag tgcattaggt ttcgcaaaca aaggattcac
     5521 tacaacttgc tttggttctg taattgtatt ttctttagct ggtacatcat gcaattttgt
     5581 tctagacatt taattcacct ccttttaaga tgttttttcg ttcttttccg ggaacgttat
     5641 tggtaaaaaa aatgtctagg ttatttgttt cataacctaa tatttttgcc attttaataa
     5701 attcattcgc tccaatatct actattccat tttctctctt tgcataaggc gttcttgttt
     5761 tccaccccat tttgtgagcc atttcatctt gcgtaatacc acaagctatt ctttccgctc
     5821 tcaatctttt taaatttagt accacgttta cacctcctat cgttctcgtt cgagaactaa
     5881 ttacaattta ccatttacgt tctcgttcgt caacaatttt tacttaaaaa aattcaaaaa
     5941 gttttttctt ccttatatat tgtattcttt tgggaacggt gttataatca aatcgttcac
     6001 aaataagaac aaatattcaa ttcaggagat acaagaaatg agaactaatg atgaaataat
     6061 cacaataatt aaaacatcaa tgaaagaaca aaatatgtca ctaagtgaat tagctcgtcg
     6121 tgtaggtgta gcaaaatcag cagtatcaag atatttaaat ttaactagag agttcccatt
     6181 gaatcgtgct gaagattttg cgaaagtact tggaataaaa acagaatatt tattaggatt
     6241 tgctgaacgc gaagaatcta caaaacaaga tactatcgcc gcgcacttag atggagattt
     6301 tacagaggaa gaattaattg aaattagaaa gtatgcggag ttagttagaa aagcacatcg
     6361 aaatcagtaa ggggttattg catgtattta tacgaaaaga tggttattga aaacaaagag
     6421 ataccaattg atgatgggaa atctttaggt aattttgagg gtctctatga caatggggtt
     6481 attttaatta ataaaaattt gtcagaaaca cgtaaagcag aagtgttata tgaggaactt
     6541 gcccaccaca agttgacgta tggcaacatt ttagaccaat caaaatggat taatagaaaa
     6601 tttgaaaatt atgcacgtag acacggcttt atctcagctg ttccattacg tgaaattgta
     6661 gaagcttata attatggcgt acgtaacttg tatgagttgt ccgagtacct acaactgagt
     6721 gaaaaataca tattagaagc gatagaacaa tataaaaaga tatacggtat tggtacccac
     6781 tacggcgagt attcaattac atttgagccg ttgagagttt ttaaatataa agaaatataa
     6841 caaaggagaa atgaaaatga ataaaatatt tagagtactc actgttagct tgtttttctt
     6901 cacattttta ataaaaaaca atctagcata tgctgatgta ggggtaatca accttagaaa
     6961 cttttatact aattatcaac cagaaacgct tcaaggagtt agttctggaa atttttctac
     7021 ttctcatcaa ttagagtata ttgatggaaa atacacttta tattcacagt ttcataatga
     7081 atatgaagcg aagagattaa aagatcataa agtagatatc tttggaataa gttactcagg
     7141 tctttgtaat acaaaatata tgtatggcgg aattacgttg gcgaatcaaa atttagataa
     7201 acctagaaat atacctatta atctctgggt caatggtaag caaaacacta tatctacaga
     7261 cgaggtttct actcaaaaaa aagaggtaac tgctcaagag attgatatta agttacgaaa
     7321 atacctacaa aacgaataca atatatatgg ttttaataaa acaaaaaaag gtcaggaata
     7381 tggatatcag tcaaaattta attctggatt taacaaagga aaaattactt tccatttaaa
     7441 taatgaaact tcttttacat acgatttgtt ttacaccgga actggtcaag cagagagttt
     7501 tttaaaaatt tacaatgata ataaaactat agatgcagag aattttcatt tggatgtaga
     7561 gatttcatat gagaagactg aataaataga atttaaggag aaaatgcaat gaaaaaatta
     7621 atagttatca tattaataaa tataataact ttaagtgtct ctaatagtgc cagcgctcaa
     7681 ggtgatatag gaattgataa tctcaggaat ttttatacaa aaaaagactt tgtagattta
     7741 aaagatgtaa aagacaatga tactcctata gctaatcaac tacaattttc aaatgtatct
     7801 tatgatttaa tttcagaatc aaaagatttt aataaattta gtaatttcaa gggaaaaaaa
     7861 cttgatgttt ttggtattag ttataatggc c
//
LOCUS       DANRDI010000117         7867 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.117, whole genome shotgun sequence.
ACCESSION   DANRDI010000117 DANRDI010000000
VERSION     DANRDI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.117"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..701)
                     /locus_tag="PZ377_002036"
     CDS             complement(<1..701)
                     /locus_tag="PZ377_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDM6358901.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGA"
     gene            862..1773
                     /locus_tag="PZ377_002037"
     CDS             862..1773
                     /locus_tag="PZ377_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDM6358902.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            1835..2680
                     /locus_tag="PZ377_002038"
     CDS             1835..2680
                     /locus_tag="PZ377_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDM6358903.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQRH"
     gene            complement(3052..4275)
                     /locus_tag="PZ377_002039"
     CDS             complement(3052..4275)
                     /locus_tag="PZ377_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDM6358904.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            4710..5765
                     /gene="lukH"
                     /locus_tag="PZ377_002040"
     CDS             4710..5765
                     /gene="lukH"
                     /locus_tag="PZ377_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HDM6358905.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
     gene            5787..6803
                     /gene="lukG"
                     /locus_tag="PZ377_002041"
     CDS             5787..6803
                     /gene="lukG"
                     /locus_tag="PZ377_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HDM6358906.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(7041..>7867)
                     /gene="sph"
                     /locus_tag="PZ377_002042"
     CDS             complement(7041..>7867)
                     /gene="sph"
                     /locus_tag="PZ377_002042"
                     /EC_number="3.1.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HDM6358907.1"
                     /translation="MYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNV
                     KKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFD
                     NDSNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNI
                     PKDETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGK
                     PEHLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK"
BASE COUNT         2958 a         1091 c         1230 g         2588 t
ORIGIN      
        1 gctcctataa ttattaggat actagttgta gttaagacta atttagaatg taaagataat
       61 ttactcaatt ttttacaatt aataaagtct atcacgacaa aatgtccaat acctccaaat
      121 attatgagta ttgagattgt aataatgaca attggatcac tagaataatc tattaagtta
      181 ttcttaaaaa gggcaaatcc agcattatta aaagctgata ctgatgtgaa taagcttaaa
      241 aataaacctt tgcctatacc aaattttggt ataaaagata aacacaaaca aatcatacca
      301 attaattcag tgactaaact ataaatagcc aagtgtttaa ttagcttaat aacaccacca
      361 ggttcgtcaa tattccatgt aaccataatc aagaatctat ttttcattga tatctttcta
      421 tttaaaaata ctagtgtcaa tagggttacg gtcacgatac ccagaccacc tatttgaatt
      481 aataatagta ttactatctc accaagtata ttaaactgtg atcctatatc aactggggac
      541 aagccagtaa ctgtaaatgc acttgaagct ataaataggg catctaaaaa agatattggc
      601 tttttaccag taaaaggtaa atacaataaa agtgcaccta tgatagttgt agagaagaaa
      661 agcattaaat aaaaatataa aggtttgtgg actttgttca ttttaatttc atactccttt
      721 atattataaa ttaataatta gataatatca taaatgaaaa tagaaatgtt actgatgtgt
      781 attactaaca ggcgttgact aattctttta atattttata attgaaaatg aatatcaatt
      841 gaaaaacaaa aggatgatat tatgaaaaga ttaattggaa ttttattatg taatttattt
      901 attttgactg cgtgttcagc atctgtcgat aaaacaagta actcgacaaa gactacagac
      961 tataaaattg aaaatggtga aacactgaaa gtaccagaga aacctaaaag agttgctgta
     1021 ttaactggat tttatgttgg tgattttata aagttaggaa tcaaaccaat tgctgtttca
     1081 gatataacta aagattcttc aattttaaaa ccttatttaa aaggggttga ttatattgga
     1141 gaaaatgatg ttgaaagagt tgctaaagca aaaccagatt taattgttgt agatgctatg
     1201 gataaaaata taaaaaaata ccaaaaaata gcgccaacaa tcccatatac atacaataaa
     1261 tacaatcata aagaaatatt aaaagaaata ggcaagttga ctaataatga agataaagct
     1321 aaaaaatgga ttgaagagtg ggacgataaa actagaaaag ataaaaaaga aattcaaagt
     1381 aaaattggtc aagcaacagc atctgtgttt gaaccagatg aaaagcaaat atacatatat
     1441 aactctacat ggggtcgtgg tttggatatt gttcatgatg cattcggtat gccaatgaca
     1501 aagcaatata aagataaatt acaagaagat aaaaaaggct atgcttccat ttcaaaagaa
     1561 aatattagta aatatgctgg tgattatata tttttaagta aaccttcgta cggaaaattt
     1621 gattttgaaa aaacacatac atggcagaat attgaagctg taaaaaaagg acatgtaatt
     1681 tcatataaag cagaagatta ttggttcaca gatcctatta cattagaaca tttgagaagt
     1741 aaattaaaaa aagaaatttt aaataaaaaa taatagaaat aagttgtaaa aattttctta
     1801 tgcattggta ctaatgtttt taaggagtga ttaaatgaag caactggttg gaattcccga
     1861 atcaatgtta attcctttga tagctcgagc aaaagagtac gaaaacgaaa aaccaataat
     1921 aaaagacgca ctatcaaaaa aaatatttga tggtttagat gatatgtaca aaaatgttac
     1981 atgtgatgac atgtctcaaa ttggaattag tatacgtact gtgataatag attgtgttac
     2041 taagaggctt atcaaggata ataaagattt aatcgtggtc aatataggtt gtggcttaga
     2101 tacaaggttt caaagattta ataaagaaaa aatatcatgg atagatttag atgtaccaga
     2161 atcaatagaa atacgaaaaa cattttttaa agaaagtaat agttataaga tgatatctaa
     2221 atctatgcta gattacagtt ggattgatga tgtcaaaaat tataaatttt ttaatagtaa
     2281 gtcaaatata ttgtttatta ttgaaggtgt attgatgtat tttgatgaga gtgtaatgac
     2341 tcaattattg gacactatta tcaaaaagat gggagatcat aatttgacat ttgcgattga
     2401 attttgctca aaaacaattg cgaataatac aaagagacat caatcggtat caaagttatc
     2461 ctcacaacct gtttttaaat atggttacaa tgatttaaaa aaattgaatg aaattttacc
     2521 aaatacaata agagtaatac atgaatataa ttattttgac tattataaga atcgatgggg
     2581 cttatttgga tattgtagat ttatacctta tttaaaaaaa aggttaaaca ataaaatagt
     2641 acttatgaaa tataaagttc caaagagaca gagacattaa gttcttagac aatgtaaaaa
     2701 agctgatttc tataaattat ttgatagaaa tcagcttttt tcaaatgtat ttgataattt
     2761 acagctcgtt gagctgctgt tttctaggga tttatgtccc attcctttta cgagaaggtt
     2821 tttacgagtt ttatgtaact attagaagct atctttttat atgaccatta gtcaatatat
     2881 gtatgacaaa tcagcacaat aatttcagtt ttacatcact taatcaatga aaatgtgctt
     2941 gttgatattt gaatatttac attcaaacaa tattcatcgc tatttctatc aacttaaaat
     3001 tcgcaataaa aaactgccag cacgcgaata gggcagctga cagcatcgtt cttatttttc
     3061 ttttaaatat tgaatggaag cctctttaaa aatatcaata tatttcagat acatatcttt
     3121 ttcaatatat tcatcgattt gatgtgccat taatggatta cctggtccaa aaatggctaa
     3181 atcaacattg tccttattat ctcctaagaa actagaggca tctgttgcac ctacaagcgc
     3241 tgaaacaaat atttcgtctt gttctacata actagaagct acatctttaa tcgtagtaat
     3301 taatttgcta tttttatcgc ttgttacagg tcggtgattg cttggaatat cgagtgaaag
     3361 cttattgcta tccacatcat taatgatatt ttggaaaaac gattctataa agtcgttatc
     3421 atactcagga actggtctta cgttaaattc aagtgaagct tcatctggta cagagttaaa
     3481 ttgtttgccg ccatttataa tcgaacatac agctgtaaga ccagatgcat aatttgcatc
     3541 ctcttcagaa atttcttttc caatcaatga tttgaacata ggcgcaacat ctaattcatg
     3601 tttagtatct tgttgtttaa gctctgaata tttttcttta aatagattat aaaattcaag
     3661 cagtgtatca attgcattgt caccaataaa tggaactgag ctatggacag ctttaccagt
     3721 tgcagttact ttacatgaca tagacccctt atgtgcataa taaattccag atccagttgg
     3781 ttcagcaata attaagctat cgacatcgtc taaatagcct ttatcagcta ataatttggc
     3841 accttcttgt tctttctctt cgccagcagt agccagtaat ctaatcgttc catgaggcaa
     3901 ttcattttgt tcttttaatt cgattagaga tacgaccaaa gccattaaac cgcctttcat
     3961 atctgtagtg cctcggccat ataatttgcc atctttttct gtcagttgaa aagggggata
     4021 tgaccaatta tcttgatttc ctgcatcaac aacatccata tgaccactca atgcgagtat
     4081 aggtgagccg ttaccgattt ctgcaacgat attggcgcgg tgttcattaa ctttcaaaat
     4141 ttcagattta atatcgtact tgtcgaataa atctgttaaa taattacaaa cgtctatttc
     4201 attattattt tcagtttgta gttcaacaat atctgctagt aattgaattt tttctttttc
     4261 actaaaagtt gtcattaagc tcacaccttt caaagtagta tatatattta tataaacata
     4321 atttttattc ttaaacataa tttaaaatgc agaaagtagt gtgttatttg atttcgttct
     4381 atgaaattaa ctgtctagta tcaacgatct tattaacgct gatatcggat atgcttaatt
     4441 ttatagtaaa ttgtatgaga aagatgcagg atattattta gctaataatt atatataaat
     4501 ttcaaatcga tggttattaa ctactaaata aaaatatttg ctaaattaat gactttgtac
     4561 acacattatt tttaaagagg ataaagtatt taataatatt aacaaaatca tttaataaat
     4621 agttaaatat atattctctg tttttgtgat attattcaca tgtcgataca tatcaacaaa
     4681 tatcaatcat acgaaagaag gttataacaa tgaaaaataa aaaacgtgtt ttaatagcgt
     4741 catcattatc atgtgcaatt ttattgttat cagcagcaac gactcaagca aattcagctc
     4801 ataaagactc tcaagaccaa aataagaaag aacatgttga taagtctcaa caaaaagaca
     4861 aacgtaatgt tactaataaa gataaaaatt caacagcacc ggatgatatt gggaaaaacg
     4921 gtaaaatcac aaaacgaact gaaacagtat atgatgagaa aacaaatata ctccaaaatt
     4981 tacaattcga ctttatcgat gatccaactt atgacaagaa tgtattactt gttaaaaaac
     5041 aaggctcaat tcattcaaat ttaaagtttg aatctcataa agaagaaaaa aattcaaatt
     5101 ggttaaagta tccaagtgag taccatgtag attttcaagt aaaaagaaat cgtaaaactg
     5161 aaatattaga ccaattgccg aaaaataaaa tttcaactgc aaaagtagac agtacatttt
     5221 catatagctc aggtggtaaa ttcgattcaa caaaaggtat tggacgaact tcatcaaata
     5281 gctactccaa aacgattagt tataatcagc aaaattatga cacaattgcc agcggtaaaa
     5341 ataataactg gcatgtacac tggtcagtta ttgcgaatga cttgaagtat ggtggagaag
     5401 tgaaaaatag aaatgatgaa ttattattct atagaaatac gagaattgct actgtagaaa
     5461 accctgaact aagctttgct tcaaaatata gatacccagc attagtaaga agtggcttta
     5521 atccagaatt tttaacttat ttatctaatg aaaagtcaaa tgagaaaacg caatttgaag
     5581 taacatacac acgaaatcaa gatattttga aaaacagacc tggaatacat tatgcacctc
     5641 caattttaga aaaaaataaa gatggtcaaa gattaattgt cacttatgaa gttgattgga
     5701 aaaataaaac agttaaagtc gttgataaat attctgatga caataaacct tataaagaag
     5761 gataatattg aaagggcgga ttactaatga ttaaacaact atgtaaaaat atcacaattt
     5821 gtacgttagc actatcgact actttcactg tattaccagc tacttcattt gcaaagatta
     5881 attctgaaat caaacaagtt tctgagaaga atcttgatgg tgatactaaa atgtatacac
     5941 gtacagctac aacaagtgat agtcaaaaaa atattactca aagcttacaa tttaatttct
     6001 taactgaacc taattatgat aaagaaacag tatttattaa agcaaaaggt acaattggta
     6061 gtggtttgag aattttagac ccaaatggtt attggaatag tacattaaga tggcctggat
     6121 cttattcagt ttcaattcaa aatgttgatg acaacaacaa tacaaatgtg actgactttg
     6181 caccaaaaaa tcaggatgaa tcaagagaag ttaaatatac gtatggttat aaaacaggtg
     6241 gagatttttc gattaatcgt ggaggcttaa ctggaaatat tacaaaagag agtaattatt
     6301 cagagacgat tagttatcaa caaccatcat atcgtacatt acttgatcaa tctacgtcac
     6361 ataaaggtgt aggttggaaa gtagaagcac atttgataaa taatatggga catgaccata
     6421 cgagacaatt aactaatgat agtgataata gaactaaaag tgaaattttt tctttaacac
     6481 gaaatggaaa tttatgggcg aaagataatt tcacacctaa agacaaaatg cctgtaactg
     6541 tgtctgaagg gtttaatcca gaatttttag ctgttatgtc acatgataaa aaagacaaag
     6601 gtaaatcaca atttgttgtt cattataaaa gatcaatgga tgagtttaaa atagattgga
     6661 atcgccatgg tttctggggc tattggtctg gtgaaaacca tgtagataaa aaagaagaaa
     6721 aattatcagc attatatgaa gttgattgga agacacataa tgtgaagttt gtaaaagtac
     6781 ttaatgataa tgaaaagaaa taagtaataa aagttgcctg ctacatagaa tgtagtaggt
     6841 aacttttatt tatatttgaa tagatagatt tattatgacg tgcagtgtat gaatcttgtt
     6901 tgagtgtaga gtaaagactt gtattaatga aaaattaaag ttgttaagat gattttatta
     6961 aaatgtaagt caattcaaat tttaacaata taactcgctt cgtcctttta gaacgaagca
     7021 agttattagt tagttgagca ctatttacta taggctttga ttgggtaatg atctgaaaaa
     7081 tcattgtaaa cgtagtaata tgggaacgca tatacatccc atggcttagg tttttcagtc
     7141 acaacttcat tgactaattg ttttggttgt ttatgatctt tatctgtaaa tatatagtct
     7201 aaatgttctg gtttaccatt agggtaatta tatttcgcaa ttgaatttga ttgagggtcc
     7261 catgtgctat tatgacctgc atatagaaca tcatttacat tcaagttttt aagcatatct
     7321 ttgaactctg gagtgccttt attgacatta aggtcgccac ctatatatac cgtttcatct
     7381 ttagggatat ttttcttttt aacaaagtca ctgatttctt tcatttgttc agctctaatt
     7441 tttcgatcat gtccagcacc acaacgtgaa tcttcagatt gtgtatgtgt accgataacg
     7501 tgaacgttct taccattttt ctctattttt gtataaacaa agcctttgtt gctatcatta
     7561 tcgaatccac aaccgctttt gaaaacatgc tggatttttt ctttaatagg atatttactt
     7621 acaatcgcta cgccaccatc ttctgcaaca gttgatgagt agctaccttc agttttgtcc
     7681 caccctgatt gagaacggcc gagtacaggt gtttgataag gatattcttt tttcacatta
     7741 cttaataatt tgtctgatgc accattatca aatgcttcat tgaatattac gacatcatta
     7801 tttttaatat aagaagattg tccgattaaa tcagcgcgtt tatattgccc ccagtttgga
     7861 tacatag
//
LOCUS       DANRDI010000118         7832 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.118, whole genome shotgun sequence.
ACCESSION   DANRDI010000118 DANRDI010000000
VERSION     DANRDI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.118"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..682
                     /locus_tag="PZ377_002043"
     CDS             <1..682
                     /locus_tag="PZ377_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDM6358908.1"
                     /translation="RKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHIYSSA
                     LKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAKEHIL
                     DIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSIPVFL
                     AERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVEDKLQ
                     QIHSEITE"
     gene            847..1611
                     /locus_tag="PZ377_002044"
     CDS             847..1611
                     /locus_tag="PZ377_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDM6358909.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            1765..2445
                     /locus_tag="PZ377_002045"
     CDS             1765..2445
                     /locus_tag="PZ377_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDM6358910.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            2469..4022
                     /gene="gntK"
                     /locus_tag="PZ377_002046"
     CDS             2469..4022
                     /gene="gntK"
                     /locus_tag="PZ377_002046"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDM6358911.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            4139..5497
                     /locus_tag="PZ377_002047"
     CDS             4139..5497
                     /locus_tag="PZ377_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDM6358912.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            5880..5981
                     /locus_tag="PZ377_002048"
     CDS             5880..5981
                     /locus_tag="PZ377_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358913.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
     gene            5965..>7832
                     /locus_tag="PZ377_002049"
     CDS             5965..>7832
                     /locus_tag="PZ377_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDM6358914.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKH"
BASE COUNT         2817 a         1141 c         1573 g         2301 t
ORIGIN      
        1 tcgaaaacca tcactatatt tagaggattt gcgacatgat tttaaaaata gcttaagtaa
       61 atttgaaaat ggtgatgaag catttgatac gttattaggt ttcgtagagt tagatcatat
      121 ttattcgtca gcactaaagg aaataagcac taaactgagt attttagatg acaatttcaa
      181 tcacatttat aaacacaatc ctatacatca tatggagcga cgtgtgaaag aaatgcgtag
      241 tttaatagaa aagcttaatc gtaaaggatt acagattagc gcagaaactg ccaaagaaca
      301 tatactggat attgccggaa ttcgcgtagt atgtaattac ttagatgata tttatttgat
      361 tgaagagatg ttgcttaaac aagaagacgt acaattgata aaacgtaaag attatattca
      421 gcaccctaaa gaaaatggtt accgcagttt acatatcgtt gtatctattc cagtcttttt
      481 agcagaacgc gttgaggtat tgcctgttga aattcaaatt agaacgatag gtatggatat
      541 gtgggcaagt ttagaacata aaatacgtta taaaaacaat gcagagacgg aaaagtatcg
      601 agatttactg aaagaatgtg cgacagagat tactgaagtt gaagataaat tacaacaaat
      661 tcattctgaa ataacagagt agatgtttag taggtgtgtt acttagtttg agtgagtttg
      721 taaatcaaca tggtacgaaa tcataaaaat aagacgccta gtataataaa tttctttaaa
      781 atgtggaaat tgtacttgct cgtgacgtcg ggtcatcaac tatggtaatt ataggaggtg
      841 gaatctatgt ctaactattc gactggagaa ctcgcaaaat tatgcaatgt gacaacacga
      901 acgattcaat attatgatcg caaaggtatt ttgaaaccac aaggatttac agaaggaaag
      961 cgtcgtgtgt atacagaaca acagcgacaa acattagagt taatcttatt gcttaaagat
     1021 ttaggttgtg cgttaagcga tatagatatg ttgctaaaag gtgaaggtac tttgaagacg
     1081 ctcaatactt tactaactat gaaacaacaa gaaattaacc aacaagttaa acagcaacaa
     1141 gcggtattaa acaaaattaa aaatgttcaa tattacgtaa atgaagcgtc gacgtctcca
     1201 atcacacact taaaagacat agagcatgtc atgagtaaat cagctgaaat gaaaagtatt
     1261 cgtcgtaaca tttggattag tgctggtatt ataggaataa ttcaatattc tagcattata
     1321 agtgcaattt tgatgaaaaa taaatggccg tttttaattg ctttaccatt tatgattggt
     1381 tatggcattg gtgttacttt ttattaccaa caaaaggttg cctatttatg tcctaactgc
     1441 cagcatatat tctcaccatc tttgtgggca gttatcaaag cgaaacatac agcgacaaca
     1501 cgtcgattcg aatgtccaaa ctgtcatgaa acgcattatt gcattgaagt acctaaagcg
     1561 catatgagta cagaacaatt agaaatatcc cacatacaac ataacaatta aatcattcgt
     1621 gtgatagtga ccatttcctt taaatttaaa tcgaaaaggg gtggtcacta ttttagtgct
     1681 cgtaaagaaa gcgcttgcaa actcataata actatattaa catacttgta tacaagtatg
     1741 tcgtgtaatg aaggagtgac tttgatgatg tatggatatc cagagaaatg gttggaaggt
     1801 atgacaactg gagaaggtat cgcggcagaa ttacgcttag gcattgtgaa tggtcacata
     1861 gctgaaggta cgttactcac tgaaaatcaa atggcaaagc aatttaatgt gagtcgttcg
     1921 ccaattcgag atgcatttaa attattgcaa caaaatcaac tcatccaatt agaaagaatg
     1981 ggtgcacatg tgttgccgtt tggggaacaa gaaaagaaag aaatgtatga tttgcgactg
     2041 atgttagagt catttgcatt ttcaagagtt aaaaatcaag agcgactacc catcgtaaaa
     2101 gaaatgaaga aacaacttga aatgatgaaa gtggcagtaa aatttgagga tgcagaatca
     2161 tttacgaagc atgactttga atttcatgaa acattaatca aagcatctaa tcatcaatat
     2221 ttaaactcat tttggagtca tttaaaacca gtaatgatgg cactcgtttt aacatccatg
     2281 cgacaacgta tgcaacagaa cccgcaagat tttgaacgca tacatcataa tcatcaagtg
     2341 tttattgatg cagtggaaca atacgacagt caaattttga aagaagcgtt tcatttaaat
     2401 ttcgacgatg ttggtaaaga tattgaagga ttttggttga attaaaatga aagaaagaag
     2461 gatttatcat gaaatatatg attggtgtcg acattggaac gacgagtacc aagtcagtct
     2521 tatatgatga aaatggaact tttatcatga aacatcaaat aggctatgat ttacacacac
     2581 caaacgttga tgtctcagaa gaaaacccag atgaattatt tgatgcagta ctaatgacta
     2641 ttaaatatgt tatgagagaa tccaaagtta ataaagacga cattaagttt gtgtcattta
     2701 gtgcgcaaat gcatagcttg attgcgatgg atcagcaaca tcaaagatta acaaataata
     2761 ttacttgggc agataaccgc gctgcaaaat atgcaacagt aataaatgaa gtgcatgatg
     2821 gcaatgcgat ttaccagcga acaggcacac cgattcatcc tatgtcgcca ttagcgaaga
     2881 ttttttggat gaaacatgaa tggcaagatg tatttcaacg tactgctaag tttgcagata
     2941 ttaaaacata cattttctat catttatttg atacatatat cattgattat tcaatggctt
     3001 ccgcaacagg gatgtttaat ttagaaacat tagattggga tgttgaggca ttagaactgc
     3061 tgggtatttc caaggaaatg ttgccggaat tagttccaac aacgtatgtg atgaaaggta
     3121 tgaaagaacg ttatgcaaca ttaatggggc ttaataaaga tacaccgttt gttattggtg
     3181 cgagtgatgg ggtcctttct aatttaggtg tcaatagtgt tggtaaagga gaagttgctg
     3241 tcacaatcgg tacatctggg gcgattcgta ctgtgataga taaaccgcgt actgattaca
     3301 aaggtagaat attttgttat gtcttaacag aggaccacta cgtcatcgga ggtcctgtaa
     3361 ataatggtgg tgtcgtattg agatggttgc gcgacgagtt gctagcgagt gaagtcgaaa
     3421 ctgcgaaacg tctcggtgtt gatccttatg atgtcttaac tcaaattgca aagcgtgtta
     3481 aaccaggtgc agatggttta atattccatc cctatttagc aggagaacgt gcgccgcttt
     3541 ggaacgcaaa tgcaagaggt tcattcttcg gtttaacttt atctcataaa aaagaacata
     3601 tgattcgcgc tgcattagaa ggtgttcttt acaatttgta taccgtctat cttgcgctta
     3661 ttgaagtaat gaatgaaaca cctaagatga taaaagcaac aggtggtttt gcgaaaagtg
     3721 aagtatggcg tcaaatgatg tcagatatat ttgacacaga gttagtggtt cctgaaagtt
     3781 atgaaagttc atgcttaggt gcctgcgtgc tcggacttaa agctgtaggt gacattgaag
     3841 atttttcaat cgtttcatcg atggtcggtg ccacaaacaa tcatacgccg attgaagaaa
     3901 atgtcactgt ttaccaagag atcgtatcca tttttatcaa tttaagtcgt tctttaacag
     3961 agaattatga acaaattgca gattttcaac gccaacatat cgctgaaaat aaaacacaat
     4021 aaatacgtca ctcaagcatc ttagataaag ttgttgtgcc atgactacta tatattatgt
     4081 ggaatacgtg cgcgacgacg gtgttacatt ctattgaaaa gagaaagagg ggaatgtcat
     4141 gtttaacgaa atatggccgt taatcagtgt tgttttaggt atcgttattt tattagtatt
     4201 aattattggg tttaaattaa atacatttat ttcattaatt atcacatcga tgattacagc
     4261 gttaatgcta ggtataccat tgactaaaat tatggagacg attgagaaag gaatgggcag
     4321 tacgctcggc cacattgcac tgatattcgg tttaggtgcc atacttggga aattgcttgc
     4381 tgatggtggt ggtgcaactc gaatcgcaga cacgttgatt caaaaatttg gtcaaaaaca
     4441 tgtacaatgg gcaatgcttg ttgcagcatt tatcgtcggt attgcattgt tctttgaagt
     4501 aggtttagtc ttactgattc cattagtatt tacagtagca aaacgcgcaa atgtttcagt
     4561 gttaaaacta ggactaccta tggtaacagc tttatcagtg acacatggct ttttaccacc
     4621 acatccagga ccggtagtca tcgcaaaaga attaaaggca aatgttggag atgtattact
     4681 gtatggtatg attattgcca ttccagttac actcattgca gggcctatat ttaacaaagt
     4741 tgcacaaaaa atgattccgt ctgcgtatac acgagaaggc gatatttcag cgttaggtgc
     4801 acaaaaagaa tttacggatc aagagatgcc aggatttggt atgagtttat taacagcaac
     4861 attaccagtc atattaatgt tagtgtctac gataacgcaa cttgtaactg ggcatgacaa
     4921 acctacaaat ctatttgaat ctatcattta tatgatagga acagcaggga cagccatgct
     4981 aatagcagta ttatttgcaa tcgttacgat gggattaatg agaaagcgta agatgaatca
     5041 tattatggaa tcagtgacga atgcgattta tccaatcgga atgatgttat tgattattgg
     5101 cggtggcggt acatttaaac aagtattaat tgacggcggt gtcggaaata caattgctaa
     5161 aatgtttgaa ggtacagaga tgtctccgat tttactagca tggattgttg cagctgtgct
     5221 acgtatcgca ttaggttcgg ctacagtagc tgcgatttca actacaggta ttgtcttacc
     5281 attattacaa tcatcagatg taaatgttgc attagttgta cttgcgatag gtgcaggtag
     5341 tgtgattttg tctcatgtaa atgacgcagg attttggatg tttaaagaat attttggttt
     5401 aactgtaaaa gaaacattct taacatggtc attattagaa acgattattt ccgtttcagg
     5461 tatcatcttc attttattca ttagcttatt tgtttaacta aatattaagt aaacgtgttt
     5521 ttaggtaatt gcaatatatt gccttgaaac acgtttattt ttatgcagaa aatcgtctga
     5581 aatactcaga atccgaacga tataaaaagt atattgggta ttttagagtg atgaaaggcg
     5641 tttcaagaca tgtttttgtg cgtattgtac aggcgataat tatgaaacgt ttagtatatt
     5701 gttttaaatt agataatgat gaatttaatt taaaaaataa gtataaaaaa tacaagcctt
     5761 gtgtgacaag ggtttctgat gacttgaata caatttatag gtatatttca aataataaaa
     5821 ttatcaatta acataaaatt aatgacaatc ttaacttttc attaactcgc ttttttgtat
     5881 tgcttttaaa aaccgaacaa tatagacttg catttattaa gtttaaaaaa ttaatgaatt
     5941 ttgcatttaa agggagatat tatagtgaaa aacaatctta ggtacggcat tagaaaacat
     6001 aaattgggag cagcatcagt attcttagga acaatgatcg ttgttgggat gggacaagac
     6061 aaagaagctg cagcatcaga acaaaagaca actacagtag aagaaaatgg gaattcagct
     6121 actgataata aaacaagtga aacacaaaca actgcaacta acgttaatca tatagaagaa
     6181 actcaatcat ataacgcaac agtaacagaa caaccgtcaa acgcaacaca agtaacaact
     6241 gaagaagcac caaaagcagt acaagcacca caaactgcac aaccagcaaa tatagaaaca
     6301 gttaaagaag aggtagttaa ggaagaagcg aaacctcaag ttaaggaaac aacacaatct
     6361 caagacaata gcggagatca aagacaagta gatttaacac ctaaaaaggc tacacaaaat
     6421 caagtcgcag aaacacaagt tgaagtggca cagccaagaa cggcatcaga aagtaagcca
     6481 cgtgtgacaa gatcagcaga tgtagcggaa gctaaggaag ctagtaacgc gaaagtggaa
     6541 acgggtacag atgtaacaag taaagttaca gtagaaattg gttctattga ggggcataac
     6601 aatacaaata aagtagaacc tcatgcagga caacgagcgg tactaaaata taagttgaaa
     6661 tttgagaatg gtttacatca aggtgactac tttgacttta ctttatcaaa taatgtaaat
     6721 acgcatggcg tatcaactgc tagaaaagta ccagaaatta aaaatggttc agtcgtaatg
     6781 gcgacaggtg aagttttaga aggtggaaag attagatata catttacaaa tgatattgaa
     6841 gataaggttg atgtaacggc tgaactagaa attaatttat ttattgatcc taaaactgta
     6901 caaactaatg gaaatcaaac tataacttca acactaaatg aagaacaaac ttcaaaggaa
     6961 ttagatgtta aatataaaga tggtattggg aattattatg ccaatttaaa tggatcgatt
     7021 gagacattta ataaagcgaa taatagattt tcgcatgttg catttattaa acctaataat
     7081 ggtaaaacga caagtgtgac tgttactgga actttaatga aaggtagtaa tcagaatgga
     7141 aatcaaccaa aagttaggat atttgaatac ttgggtaata atgaagacat agcgaagagt
     7201 gtatatgcaa atacgacaga tacttctaaa tttaaagaag tcacaagtaa tatgagtggg
     7261 aatttgaatt tacaaaataa tggaagctat tcattgaata tagaaaatct agataaaact
     7321 tatgttgttc actatgatgg agagtattta aatggtactg atgaagttga ttttagaaca
     7381 caaatggtag gacatccaga gcaactttat aagtattatt atgatagagg atatacctta
     7441 acttgggata atggtttagt tttatacagt aataaagcga acggaaatgg gaaaaatggt
     7501 ccgattattc aaaataataa atttgaatat aaagaagata caattaaaga aactcttaca
     7561 ggtcaatatg ataagaattt agtaactact gttgaagagg aatatgattc atcaactctt
     7621 gacattgatt accacacagc tatagatggt ggaggtggat atgttgatgg atacattgaa
     7681 acaatagaag aaacggattc atcagctatt gatatcgatt accatactgc tgtggatagc
     7741 gaagcaggtc acgttggagg atacactgag tcctctgagg aatcaaatcc aattgacttt
     7801 gaagaatcta cacatgaaaa ttcaaaacat ca
//
LOCUS       DANRDI010000119         7813 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.119, whole genome shotgun sequence.
ACCESSION   DANRDI010000119 DANRDI010000000
VERSION     DANRDI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.119"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            126..1502
                     /locus_tag="PZ377_002050"
     CDS             126..1502
                     /locus_tag="PZ377_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDM6358915.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            1504..2643
                     /locus_tag="PZ377_002051"
     CDS             1504..2643
                     /locus_tag="PZ377_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDM6358916.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            2618..2983
                     /locus_tag="PZ377_002052"
     CDS             2618..2983
                     /locus_tag="PZ377_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDM6358917.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            2986..3255
                     /locus_tag="PZ377_002053"
     CDS             2986..3255
                     /locus_tag="PZ377_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358918.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(3456..3623)
                     /locus_tag="PZ377_002054"
     CDS             complement(3456..3623)
                     /locus_tag="PZ377_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDM6358919.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(4130..4960)
                     /gene="thiD"
                     /locus_tag="PZ377_002055"
     CDS             complement(4130..4960)
                     /gene="thiD"
                     /locus_tag="PZ377_002055"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDM6358920.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            5144..5800
                     /locus_tag="PZ377_002056"
     CDS             5144..5800
                     /locus_tag="PZ377_002056"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDM6358921.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKSVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            5801..6181
                     /locus_tag="PZ377_002057"
     CDS             5801..6181
                     /locus_tag="PZ377_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDM6358922.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            6314..6682
                     /locus_tag="PZ377_002058"
     CDS             6314..6682
                     /locus_tag="PZ377_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDM6358923.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            6803..>7813
                     /locus_tag="PZ377_002059"
     CDS             6803..>7813
                     /locus_tag="PZ377_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDM6358924.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMF"
BASE COUNT         2645 a         1188 c         1449 g         2531 t
ORIGIN      
        1 tatcaaattt atctagggct taagtttgat ttttataata ggcaggttta cctgataaaa
       61 atacttattc attatataat gttaacaata tgtattttaa agtttacatt gagtgaggga
      121 tattgatgaa cgtaatttta gaacagttga aaacacatac tcaaaataaa cctaatgaca
      181 tagcattaca tatcgatgat gaaacaatta catatagtca actaaatgcc cgcatcacta
      241 gcgcagttga atctttgcag aaatattcac ttaaccctgt cgttgctatt aatatgaaat
      301 caccggtgca aagtattatt tgttatttag ctttgcatcg tttacataaa gtgcctatga
      361 tgatggaagg taaatggcaa agtactatac atcgtcaatt gattgaaaaa tatggtatta
      421 aagatgtaat tggagataca ggtctcatgc agaatataga ctcaccgatg tttattgatt
      481 caacgcaatt acagcactac cccaatttat tacatattgg ttttacttca gggacaactg
      541 gactgccaaa agcatattat cgtgatgaag attcatggtt ggcttctttt gaagttaatg
      601 aaatgttgat gttaaaaaat gaaaatgcaa tagcagcccc tggaccacta tcgcactcgt
      661 taacattata tgcgttattg tttgctttaa gttccggtcg tacttttata ggacagacca
      721 cttttcatcc tgaaaagtta cttaatcaat gtcataaaat atcatcatac aaagttgcta
      781 tgtttcttgt tccaacgatg attaaatcat tattgttagt ttacaacaat gaacatacaa
      841 tccaatcatt ttttagcagt ggagataagc tgcattcttc tatttttaaa aagataaaaa
      901 atcaagcaaa tgacataaat ttgattgaat tttttggtac atcggaaacc agttttatca
      961 gctataactt gaatcagcaa gcaccagttg aatcagtagg tgtgctattt ccaaatgtgg
     1021 aattgaaaac aacgaatcac gatcacaatg gtataggaac tatttgtata aaaagtaata
     1081 tgatgtttag tggctatgta agtgaacaat gtataaataa tgatgaatgg tttgttacta
     1141 atgataatgg ctatgtaaaa gagcagtatt tatatttaac gggacgtcaa caggatatgt
     1201 taattattgg tggtcaaaat atatatccag cacatgttga acgcctttta acgcaatctt
     1261 cgagcattga tgaagcaatt atcatcggta ttccaaatga gcgttttggt caaataggcg
     1321 tattgcttta ttctggtgat gtgacactta cacataaaaa tgtaaaacaa tttttaaaaa
     1381 agaaagtgaa acgctatgaa attccatcga tgattcatca tgtagaaaag atgtattaca
     1441 ctgcaagtgg taaaattgct agagaaaaaa tgatgtcgat gtatttgaga ggtgaattat
     1501 aatatgaatc aagcagtcat agttgcagct aaacgaactg catttgggaa atatggtggc
     1561 actttaaaac atttagagcc agaacaattg cttaaacctt tattccaaca ttttaaagag
     1621 aagtatccag aggtaatatc taaaatagat gatgtagttt taggtaatgt tgttgggaat
     1681 ggtggcaata ttgcaagaaa agcattgctt gaagcggggc ttaaagattc aatacctggc
     1741 gtcacaatcg atcggcaatg tgggtctgga cttgaaagtg ttcaatatgc atgtcgcatg
     1801 atccaagccg gagctggcaa ggtatatatt gcaggtggtg ttgaaagtac aagtcgagca
     1861 ccttggaaaa tcaaacgacc gcattctgtg tacgaaacag cattacctga gttttatgag
     1921 cgtgcatcat ttgcacctga aatgagcgac ccatcaatga ttcaaggtgc tgaaaatgtg
     1981 gccaagatgt atgatgtttc aagagaatta caagatgaat ttgcttatcg aagtcatcaa
     2041 ttgacagcgg aaaatgtaaa aaatggaaat atttctcagg aaatattacc tataaccgtt
     2101 aaaggagaaa tattcaacac tgatgaaagt ctaaaatcac atattccgaa agataacttt
     2161 ggccgattta agcccgtgat caaaggtggg accgttaccg ctgcgaatag ttgtatgaaa
     2221 aatgatggtg cagttttatt gcttattatg gaaaaagata tggcatacga attaggtttc
     2281 gagcatggtt tattatttaa agatggtgtt acggtaggtg ttgattctaa ttttcctggc
     2341 attggtccag taccagccat ttccaactta ctaaaaagaa atcaattaac gatagaaaat
     2401 attgaagtca ttgaaattaa cgaagcgttc agtgcacagg tagttgcctg ccaacaagct
     2461 ttaaatattt caaatacgca attaaatata tggggtggtg cattagcatc aggtcatcca
     2521 tacggtgcaa gcggtgccca attagtgact cgattatttt atatgtttga caaagagact
     2581 atgattgcat ctatggggat agggggaggt ctaggaaatg cagcattatt tactcgattc
     2641 taaccagcga ttaaatgtgt cattttctaa ggatagtgtg gctgcatatt atcagtgttt
     2701 taaccaacct tatagaaaag aagtaccacc attaatgtgt gcgtcattat ggccaaaatt
     2761 tgatttattt aaaaaatatg caaatagcga actgatttta acaaaatcag caattaatca
     2821 aactcaaaag atagaagtag acacaatata tgtagggcat ttagaagata ttgaatgccg
     2881 acagactcgc aatatcacac gttatacaat ggctttaaca ttaactaaaa atgatcaaca
     2941 tgtcataacg gttacacaaa cttttattaa ggcgatgaag tagagatgaa gtttaatgag
     3001 atatggataa atgaatattt ggcgctcgta aatgatgata atccaataca taatgagatt
     3061 gtgccaggac aattagtgag tcaaatgatg ctgatggcta tgtcattaga gacaaaccag
     3121 tgtcaaatta actacgttaa acctatttta ataaatgaaa atatcgaatt cattgaacaa
     3181 cacgaacacg aaattatagc aattaatgac gatggagaga ttaaaataaa aatttctttg
     3241 agcacaaaaa aataaccgat attagctgca tgaacgcata ttaattagga gatgaaagga
     3301 cagctaatat cagttatgta ttgttattat tattgggaac agagatgaat ataggttacg
     3361 tttctttctt tgcacgggga tgcattaatc taaaataata ataacaacta tatcaatgtt
     3421 taataaattc tggattattg gaacgattag tcaatttaac taactttcat atgatctata
     3481 tcgtcttgta ataaagagag caatttgaat atttcagtat cactaaatga atcgtcacat
     3541 ttaattgaaa catgctgaaa cgttttggtt ataatttcat aaactggtgc gccttcatgg
     3601 tgatactgtc gataaataat cataacctat attacctcct ttgctactct atggttatat
     3661 tataaataac atttttatgt gtgacatcaa ccttaagtat caacttttta tcagacatag
     3721 aacgtatgat ttactaagac tatttatgta taaaagttct aaataaatat atatttatag
     3781 agtcgcctgg cagtcatttg ggaaatataa catatatgat tagagaggca tctatcgcaa
     3841 aagaatgata atgatagagg tattgagcat atagatgagt ttaagttcat cttgaaaata
     3901 aagggttatt tagtcataga tgtagatgta taggaaatat ttgtatgtat tgttcgatat
     3961 gtatgaaatt ttcaataaaa gctaataacg cttatatgta actttcaaat ttaaattata
     4021 tacagagcat gatgattata aaaaaataac cacatcacat aaattgagtt catacccaat
     4081 ttaagtggtg tggctaataa tgttgattta tagatgaacc gcctaatcgt taaacctctg
     4141 ttacttcaac atcgatatgt tcaatacggt tgtatgcacc gtgatccaca ggaccaacaa
     4201 aatcattcat tttccaaccg tttttaatag cagaagcgac gaaagctttc gcgctaatca
     4261 cagcttcttt cggtgactta ccgttagcta aatatgcagt tgttgccgca gcaaatgtac
     4321 aaccagcacc atggttataa ctttgttgga acatgtctgt tgttagttga taaaatgttt
     4381 gaccatcata gtataagtca tacgatttat cttgatctaa agctttgcca cctttaatga
     4441 tgacatgctg tgcgccttta tcaaagataa ttgttgcagc ctttttcata tcttcaattg
     4501 aatttaattt acctaatcct gataattgac ccgcttcaaa taagtttggt gtcactaccg
     4561 ttgctttagg tagtaaatat ttaatcatcg cctcagtatt tccaggatta agcacttcat
     4621 cttcgccttt acaaaccatg acaggatcta ctacaaaata ttgtgcatta gatgcctcat
     4681 atacttctcc agcacgtttg attatctcct cagtacctaa catacctgtt ttaatagcat
     4741 caggtccgat tgataaagcc gtttcaagtt gtttttcaaa tacatccatt ggtaatggtg
     4801 taacatcgtg tgaccatgta tctttatcca tagtaacgat ggcagttaaa gcgaccatgc
     4861 catacgtatc taattcttgg aacgttttca aatctgcttg catacctgcg ccagcacttg
     4921 tgtcagaacc ggcaattgtt aaaactttct ttaaagccat tgagcttcac tcctacataa
     4981 taatattgta ttcatcatat catttttaac ctaattgaaa aatattaagc attcaatatt
     5041 tgatgattat tgaaatgaat cattcatact attgtaactt ttgaaaatgt cattcacttt
     5101 agataagtgt gatatgttaa aatatgtcct gaggtgagat tgaatggaat ggtcgcaaat
     5161 ttttcatgac ataacaacga aacatgactt taaagctatg catgattttt tagaaaaaga
     5221 atattcgact gcaatcgtat accctgatag ggaaaatata tatcaagcgt ttgatttaac
     5281 accgtttgaa aatatcaaag ttgttatatt aggacaagac ccgtatcatg gtccaaacca
     5341 agcacatgga ttagcatttt cagtgcaacc taacgcaaaa ttccctccat ctttacgtaa
     5401 tatgtataaa gaattagcag atgatattgg atgcgttaga caaacaccgc atttacaaga
     5461 ttgggcaaga gaaggcgtct tgttattgaa tacagtttta accgtaagac agggtgaagc
     5521 aaattctcat cgtgatattg gttgggaaac atttactgat gaaattatta aatcagtgtc
     5581 tgattataaa gaacatgttg tctttatttt gtgggggaaa cctgcacagc aaaaaataaa
     5641 gcttatcgat acatctaaac attgtattat aaaatcagtg catcctagtc cactgtctgc
     5701 atatagagga ttctttggat caaaaccgta ttccaaagcg aatgcctatt tagagtcagt
     5761 aggaaaatca ccaattaatt ggtgtgaaag tgaggcgtag atgttgaata gagaaacttt
     5821 aatagcacga attgagcaag aattagtaca agcagagcag gcacagcatg accatgactt
     5881 tgaaaaacat atgtatgcca tacatatatt aacatcttta tatgcttcaa catcaaatac
     5941 accacatatt ggtgaacaac aaatgaatcg tcgtattgct aaccataatc aaatgccaca
     6001 atcacaaata acgcagccaa ctcatcaagt gacagttgct gaaattgaag cgatgggtgg
     6061 taaagtaaat acgcattcag cacatcatca taataagtca tattcacaac cttcaaacca
     6121 acaacaaaga ttagcgacag atgatgacat tggcaatggt gaatccatat ttgattttta
     6181 aaaagcaaca atgaaacata attacttaat agcttgttaa gtatgtaggt taataatcaa
     6241 gacgcatata cttttattcg agtgttcgga tttaaacatt tattaatact gaattatata
     6301 aggagaggta gcaatgaaat tatttattat tttaggtgca ttaaacgcga tgatggctgt
     6361 cggtacaggt gcatttggtg cgcatggttt acaaggaaaa ataagtgatc actatttatc
     6421 agtatgggaa aaagcaacga cgtatcaaat gtaccatggc ttagcattat taattatagg
     6481 tgtaattagt ggtacaactt caatcaatgt taactgggct ggctggttaa tatttgctgg
     6541 tattattttc tttagtggat cattatatat tttagtatta actcaaatta aagttttagg
     6601 tgcgattacg ccaattggtg gcgtattgtt catcattgga tggataatgt taatcattgc
     6661 gacattcaaa tttgctggtt aaattttaaa actttagatt acctatgtaa ctaaacatta
     6721 aatttttaat aaaaataatc aagaaaaaga gttacaaact catcttttgg gtatagaata
     6781 ccttcgaggt gagtttttat ttatggaaaa aaagaataag caaatagata gaggcgattt
     6841 aaaacaaaac ctatctgaaa agtttgtatg ggcgattgca tatggttcat gtatcggatg
     6901 gggcgcattc atcttaccag gagactggat taagcagtca ggtccgattg cagcatcaat
     6961 tggtatagtt attggtgcat tattaatgat attaattgcg gttagttatg gcgcattagt
     7021 agagagattt ccagtatcag ggggcgcgtt tgcctttagt ttcttaagtt tcggcagata
     7081 tgtgagtttc ttctcatcat ggtttttaac ttttggttat gtctgtgtcg ttgctttaaa
     7141 tgcgaccgca ttcagtttac tagttaaatt cttattgcca gatgtcttaa ataatgggaa
     7201 actatacacc attgcgggct gggacgttta tattacggaa atcattattg cgaccgtatt
     7261 actacttgta ttcatgctag taacgattcg tggcgcaagt gtatctggat cattacaata
     7321 ttatttctgt gtggcgatgg taatcgtcgt attattgatg ttctttggtt cattctttgg
     7381 taataatttt gcacttgaaa atttacaacc gttagctgaa cctagcaaag gatggttagt
     7441 gtctattgtg gttattgtat ccgtggcacc atgggcatat gttggatttg ataatattcc
     7501 acaaacagca gaagagttta actttgcacc aaacaagaca tttaagctta tcgtgtacag
     7561 tttattagca gcatcattaa cttatgttgt catgatttta tacactggtt ggttatcaac
     7621 aagtcatcaa agtttaaatg ggcagttgtg gttaacaggt gctgttacac aaacagcatt
     7681 tggttatatt ggattaggtg tattagcaat tgcaattatg atgggtatat ttactggttt
     7741 aaatggattc ttgatgagtt caagtcgctt gttattttct atgggacgtt caggtattat
     7801 gccaacaatg ttt
//
LOCUS       DANRDI010000120         7693 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.120, whole genome shotgun sequence.
ACCESSION   DANRDI010000120 DANRDI010000000
VERSION     DANRDI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.120"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..160)
                     /locus_tag="PZ377_002060"
     CDS             complement(<1..160)
                     /locus_tag="PZ377_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HDM6358925.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYI"
     gene            complement(442..1320)
                     /locus_tag="PZ377_002061"
     CDS             complement(442..1320)
                     /locus_tag="PZ377_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDM6358926.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(1333..1998)
                     /gene="bshB2"
                     /locus_tag="PZ377_002062"
     CDS             complement(1333..1998)
                     /gene="bshB2"
                     /locus_tag="PZ377_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDM6358927.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(2012..2374)
                     /locus_tag="PZ377_002063"
     CDS             complement(2012..2374)
                     /locus_tag="PZ377_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HDM6358928.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            2650..3408
                     /gene="nagB"
                     /locus_tag="PZ377_002064"
     CDS             2650..3408
                     /gene="nagB"
                     /locus_tag="PZ377_002064"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDM6358929.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIVLLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3485..4117
                     /gene="hxlA"
                     /locus_tag="PZ377_002065"
     CDS             3485..4117
                     /gene="hxlA"
                     /locus_tag="PZ377_002065"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDM6358930.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            4119..4667
                     /gene="hxlB"
                     /locus_tag="PZ377_002066"
     CDS             4119..4667
                     /gene="hxlB"
                     /locus_tag="PZ377_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDM6358931.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            4780..5427
                     /locus_tag="PZ377_002067"
     CDS             4780..5427
                     /locus_tag="PZ377_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDM6358932.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            5929..7329
                     /locus_tag="PZ377_002068"
     CDS             5929..7329
                     /locus_tag="PZ377_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6358933.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
BASE COUNT         2622 a         1163 c         1354 g         2554 t
ORIGIN      
        1 taatataatt ttgggcattt ctatataaat atctattcca tgacagaaat acacattgcg
       61 ctggtcttcc catttcttta aataaattta aacgattaat aattgctttc tctatcccag
      121 ttaaattaac acctaaacta ttacctacaa aataattcat ttacaacacc acttatatct
      181 attttttata attatatcac ataatattta attacttctt ttaactggaa gatgtgttta
      241 tttataaaac aacaaatttt gatatttata atgatagtag ttattcaatc aactacgacc
      301 aatatatcat tgtagagctt aggatattga tttatgactc aggcacatca aatgagaaga
      361 tttataaaag agatatacaa ctctagaagg tataataaaa acgcgcaact aatgttacgc
      421 gtttgaatta atcatatgat attatttgcg atactttaat ttagcgaaag catcatgttg
      481 atggatagac tcttcattac gacattcgat atcgaaaccg tctaaccaat caaattcaac
      541 taagtccgcg gcaattaaac gaattaagtc ttcgacaaaa cgtggatttt catatgcacg
      601 ctctgtcaca cgtttttcat caggacgttt taaaataggg tatagaattg aacttgcatt
      661 agcttccatt gcatctaaaa ttttattttt atagtcatca actatgtctt gatctttatt
      721 aatatatgtt ttaacagtga caacaccacg ttggttgtgc gctgaatact cacttatttc
      781 ttttgaacaa gggcatagcg ttgtgacagt tgcttcaata gtaagttctt tacgtgtaac
      841 tttatcaccg tcaattgcta atccataagt gacatcggca ttaccaactg ctttaatatt
      901 tgtggttgga ctatagcgat caaagaacca tttcccagaa acatcaacgc ctgccgcatt
      961 ttgtttcata ttcgtttgta aagtgcgtaa cacctgataa agtgtattaa attcaagttc
     1021 aataccatta tcatagtgct tttcaacact ttcgattata cggctcatat taataccttt
     1081 ttcgtctttt gttaaacttg ttgaaaaact aaatgtgcca gctgtttgat actggtcaac
     1141 aagtacaggg tacactaagt ttttaatacc aacttcttct atttcaaata aaaaatcttt
     1201 atgtgtactt tgtaaatctg tcatttcgtt cttagtagta ggtttcgtgc cttcaatagg
     1261 atctacggaa ccaaagtgtt tccaacgacc ttctcgtgtc gataaatcaa attcagtcat
     1321 ttttttcctc cgttaagatt taaagtgata tgtccaatat ggttcgactg ttaaaaagct
     1381 gtgttgttta ccatcgattt caggacttgc taattgtttt aaaaatggac ctgtttgaga
     1441 agcatgtgct tcaaatgcct taattttaag ttctttaaaa tctgtaatat cattttgaat
     1501 atcaggttct ccaagagctt cggttgcatc attactgaac gcaactaaag ttaaacgagg
     1561 gcgttcttct ttaggcatgc gttcaaccgt tcgaattaca gcgtctgctg ttgcttcgtg
     1621 atcaggatgt actgcatatc caggataaaa tgaaataatc aatgatggat ttgtatcatc
     1681 gattaaagat ttaatcatac catctatatg ttcatagggt tcaaattcga cagttttgtc
     1741 acgtaaaccc atttttctta aatcagtaat accgataact ttacaagctt cttctagttc
     1801 acgctcacga atacttggta atgattcgcg tgttgcaaat gggggattac ctaaatttct
     1861 gcccatttgt cctagggtta aacatgcata tgttacaggt atgccttttt ggatataatt
     1921 tgctaatgtg cctgcagatg agaaggtttc atcatcagga tgtggaaata ttactaatac
     1981 atgtctttcg tcagtcatgt tgatgcctcc tctataaatt aaatggtcgc tcactaattt
     2041 gaagtgctgc agcgagttga ccttcgtaat taaaacctgc aattaaaaat tcatcatgct
     2101 cattgacctc aaaatgcgtt agaccttgta cataaaccca accaccattt gatagtttaa
     2161 gaccaatgcg ataaggttct ttattaccac cttttagttg tgcatgcgta tatgttattt
     2221 gtatgtttct taaaaaagta ccagcattaa aaacacgttg atcgaaatgg ttcgcatagg
     2281 ccccatttgt cgtttcaaca tgcagataca caggtttatg ttcaaaagaa gcaagtaaat
     2341 ctataacttc ttgttcttta attggttcca acacgttcac tccttacact atcaatgtgt
     2401 ttatctttct attttactaa aaactattcg ataattgtat acgattgctc aattatttat
     2461 aaattaattt tcatgaaggg taattactca ggattacgta atcatacagc attagttttt
     2521 tacttttaaa aatcaaaaat ttgttggaat ttgaaaagtg ttaaacatta aaaatgatgc
     2581 tatattaatg gtgtatgaat gaattcataa gtttttaaaa tgtattaaat ttgtggaggc
     2641 atgtaaacaa tgaaagtatt aaacttagga tcgaaaaaac aagcatcatt ctatgttgca
     2701 tgtgagttat ataaagagat ggcatttaat cagcactgta aactaggttt agcaactggt
     2761 ggtacaatga cagatttgta tgaacaactt gttaagttat taaataaaaa tcagttaaac
     2821 gtagacaatg tatccacgtt taatttagac gaatatgtag gtttaaccgc atcacatccg
     2881 caaagttatc actattatat ggatgacatg cttttcaaac aatatcctta ttttaataga
     2941 aagaacattc atattccaaa tggagatgcc gatgatatga atgcggaagc gtcaaaatat
     3001 aatgacgttt tagaacaaca aggtcaacgt gatattcaaa ttttaggtat tggtgaaaat
     3061 ggtcatattg gatttaatga acctggtacg ccgtttgata gcgttactca tatcgttgat
     3121 ttgactgaaa gtactattaa ggctaatagt cgatatttta aaaacgaaga tgatgttcca
     3181 aagcaagcca tttcgatggg acttgctaat attcttcaag ccaaacgtat cgttttactc
     3241 gcatttggtg aaaagaaacg tgctgctatt acacatttat taaatcagga aatttctgtt
     3301 gatgttccag ccacattact tcacaaacac ccgaatgttg agatatattt agacgacgaa
     3361 gcttgcccga aaaatgttgc gaaaattcat gtcgatgaaa tggattgatt gcaatgttta
     3421 attaagaaat gcctcgggaa aggttccaat agaaagataa aaagcattgg aaggatgatt
     3481 tttagtggaa ttacaattag caattgattt attaaacaaa gaagacgcgg ctgagttagc
     3541 aaataaagta aaagattatg tagatatcgt agaaatcggt acgccaatca tttacaacga
     3601 aggtttacca gcagttaaac atatggcaga caacattagt aatgtaaaag tattagcaga
     3661 catgaaaatt atagatgcag ctgattatga agttagccaa gcaattaaat ttggcgcgga
     3721 tgtaattaca atactaggtg ttgcagaaga tgcatcaatt aaagcagcta ttgaagaagc
     3781 tcataaaaat aataaacaat tactagttga tatgattgct gttcaagatt tagaaaaacg
     3841 tgcaaaagaa ctagatgaaa tgggtgctga ttatattgca gtacacactg gttatgattt
     3901 acaagcagaa gggcaatcac cattagaaag tttaagaacc gttaaatctg ttattaaaaa
     3961 ttctaaagtt gcagtagcag gtggaattaa accagataca attaaagata ttgtcgctga
     4021 aagtcctgat cttgttattg ttggtggcgg aatcgcaaat gcagatgatc cagtagaagc
     4081 tgcaaaacaa tgtcgcgctg caatcgaagg taagtaatat ggctaaattt agtgactatc
     4141 aattaattct agatgaatta aagatgactt tgtcacatgt tgaagcggat gagttttcaa
     4201 cttttgcatc caaaatacta catgctgaac atatatttgt agctggcaaa ggacgttcag
     4261 gattcgtggc gaatagtttt gcaatgcgct taaatcagct cggcaaacag gcacatgttg
     4321 ttggagaatc aacgacacct gcgattaagt cgaatgatgt atttgtaatt atctctggtt
     4381 caggttccac ggaacattta agattattag cagacaaagc aaaatcagta ggtgctgaca
     4441 tcgtattaat tactacaaat aaagattctg caataggcaa tctagctggg acgaacatcg
     4501 ttttgcctgc aggtacaaaa tatgatgaac aaggctcggc acaaccatta ggaagtttgt
     4561 ttgaacaagc atctcaatta tttttagata gtgttgtaat gggattgatg actgaaatga
     4621 atgttacgga acaaacgatg caacaaaatc atgctaattt agaataaaat aaagatagtc
     4681 gataatatga tgcctaggca gaaatattat cgattatttt tttatttaaa taataaatta
     4741 tagtataata tcaataataa acgaataggg gtgttaatat tgaagtttga caattatatt
     4801 tttgattttg atggtacgtt ggcagacacg aaaaaatgtg gtgaagtagc aacacaaagt
     4861 gcatttaaag catgtggctt aacggaacca tcatctaaag aaataacgca ttatatggga
     4921 atacctattg aagaatcatt tttaaaatta gcagaccgac cattagatga agcagcatta
     4981 gcaaagttaa tcgatacatt tagacataca tatcaatcta ttgaaaagga ctatatttat
     5041 gaatttgcgg gtataactga agccattaca agtttgtata accaagggaa aaaacttttc
     5101 gtggtgtcta gtaagaagag tgatgtatta gaaagaaatt tatcggctat tggattaaat
     5161 cacttgatta ccgaagctgt tggatccgat caagtaagtg catataaacc aaatcctgaa
     5221 ggcatacaca caattgtgca acgctacaat ttaaatagcc aacaaacggt gtatattggt
     5281 gattcaacgt ttgatgttga gatggcacaa cgtgctggta tgcaatctgc agctgtcact
     5341 tggggtgcac atgatgcaag gtcattactt cattcaaatc cggattttat tattaatgat
     5401 ccatcagaaa ttaataccgt attataaaac ttgttaaaac agagaatacc atggttaaaa
     5461 tgcatattca taaatattag attatactta gaaatatttc gctttagatt aggaatttaa
     5521 aataaatatt tattaaacat tatgaatttt taaagagtaa tgtctgactc gttgataatt
     5581 tatttttgta aaaataaatt aaagtaatga caaagttatt gaagtaaatt gagtataaac
     5641 atttaaatac gatgtcgaaa atggcgatag catatcactt acatgaagtt atgtgctatc
     5701 gctattttta gttataattc caaaaagtta atcgttcgat gatttaagaa ttattattgt
     5761 ttaattcaaa tgtatgaggg tataaaatca ttgaatttaa ttcgataaag cgaaattttt
     5821 gaacaaacat acttttgtat ttatataaaa gtttaaattc ttataaattt gacaaaacta
     5881 attaactccg tataattatg aaacatacaa gagggagtgt atgaattcat ggattttaat
     5941 aaagagaata ttaacatggt ggatgcaaag aaagctaaaa aaaccgttgt tgcaaccggt
     6001 atcggtaatg caatggaatg gttcgatttt ggtgtctatg catatacaac tgcgtacatt
     6061 ggagcgaact tcttctctcc agtagagaat gcagacattc gacaaatgtt gactttcgca
     6121 gcattagcca ttgcgttttt attaagacca attggtggtg tcgtatttgg tattattggt
     6181 gacaaatatg gacgtaaagt tgtattaaca tctacaatta ttttaatggc attttcaaca
     6241 ttaaccattg gattattgcc aagctatgat caaattggac tttgggcacc aatactatta
     6301 ttgcttgcaa gagtactaca agggttttca acaggtggag agtatgcggg ggcaatgaca
     6361 tatgttgccg aatcatctcc agataagcgt cgtaactcat taggtagtgg actagaaatt
     6421 gggacattat caggttacat agctgcttca attatgattg ctgtattaac attcttttta
     6481 acagatgaac aaatggcatc atttggttgg agaatcccat tcttactcgg tttattccta
     6541 ggattattcg gcttatattt acgtcgtaag ctggaagaat caccagtttt cgaaaatgat
     6601 gttgcaacac aaccagaaag agataacatt aactttttac aaatcatcag attttattac
     6661 aaagatatat ttgtatgttt tgtagctgtt gtattcttca atgttacaaa ctatatggta
     6721 actgcatatt taccaaccta tttagaacaa gttattaaat tagatgcaac gacaacaagt
     6781 gtattaatta cttgtgtcat ggcaataatg attccattag cattaatgtt tggtaagtta
     6841 gcggataaaa taggtgaaaa gaaagtattt ctaattggta ctggtgggct aacattattc
     6901 agtatcatcg catttatgtt attacattca caatcatttg ttgtaatagt aatcggtata
     6961 tttatattag gatttttctt atcaacttac gaagcgacaa tgccagggtc gttaccaacg
     7021 atgttttaca gtcatataag atatcgaact ttatcagtaa catttaatat ctctgtttcg
     7081 atatttggtg gtacgacgcc attagttgca acatggttag ttacgaaaac tggagatcca
     7141 ttagcacctg cgtattattt aacagcaatc agtgttattg gctttttagt tattacattc
     7201 ttacatttaa gtacagcagg aaaatctcta aaaggttcgt atccaaatgt agataacgag
     7261 caagatagag cttattatgc agaacatcca aaagaagcat tatggtgggt taaagaacgt
     7321 aagaattaga gattttaata aaaagtataa atcaatcgta tataagcact ttaaagctag
     7381 taggttctgc taactttaaa gtgcttttta aattgagaac tgtaattagc cgtaataaag
     7441 tttttgtata tacataaacc cccactgcaa tgattatcgc aatgggggaa agaggggact
     7501 taaagcatat gtttagcttt gaatacttaa aattctcttg ctattgaaat gttaggatgt
     7561 aaatatgtct tagagtattt tgtccaacgc aattaatatt gagactctaa ccttcaatat
     7621 tattatagag aacacaaact taaatagatt gggtgactta tttgtgtcag ttattgcgat
     7681 tgcgataact tct
//
LOCUS       DANRDI010000121         7677 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.121, whole genome shotgun sequence.
ACCESSION   DANRDI010000121 DANRDI010000000
VERSION     DANRDI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.121"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1822)
                     /locus_tag="PZ377_002069"
     CDS             complement(<1..1822)
                     /locus_tag="PZ377_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358934.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALI"
     gene            complement(1819..2574)
                     /locus_tag="PZ377_002070"
     CDS             complement(1819..2574)
                     /locus_tag="PZ377_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358935.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(2682..3569)
                     /gene="nsaS"
                     /locus_tag="PZ377_002071"
     CDS             complement(2682..3569)
                     /gene="nsaS"
                     /locus_tag="PZ377_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDM6358936.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(3580..4245)
                     /gene="nsaR"
                     /locus_tag="PZ377_002072"
     CDS             complement(3580..4245)
                     /gene="nsaR"
                     /locus_tag="PZ377_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDM6358937.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(4271..4471)
                     /locus_tag="PZ377_002073"
     CDS             complement(4271..4471)
                     /locus_tag="PZ377_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358938.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            4669..6093
                     /locus_tag="PZ377_002074"
     CDS             4669..6093
                     /locus_tag="PZ377_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDM6358939.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6163..6273
                     /locus_tag="PZ377_002075"
     CDS             6163..6273
                     /locus_tag="PZ377_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358940.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(6376..6831)
                     /locus_tag="PZ377_002076"
     CDS             complement(6376..6831)
                     /locus_tag="PZ377_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDM6358941.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(7076..>7677)
                     /locus_tag="PZ377_002077"
     CDS             complement(7076..>7677)
                     /locus_tag="PZ377_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDM6358942.1"
                     /translation="YMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEV
                     YDYVHQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDL
                     EWNDFSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDF
                     IDYLSRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
BASE COUNT         2583 a         1336 c         1128 g         2630 t
ORIGIN      
        1 gaatcagtgc tactgttatt ggtattgcga acaagaaagt catttgattt cgtgttatct
       61 ttgctattct tccattatct aaaccaagtt ttttcataat gtcataattg tgctgatttg
      121 cataggcttc tgagacttgt ttataataca tcattagaaa tattccaatg acaagtacaa
      181 ttgatactac gccacctaca aatattagtc cacctgtcaa acgatcccaa accattagca
      241 tttctgattt agaatttatt gacgcatcgt attttttcga tagcttttgt atttgtgatg
      301 ttgttaattt cgtctttcca aatacattaa agtttatcga tattgtcgaa ttcttttgtt
      361 catgtgcata aaatcctttt actttttgta attgattgtt atctttaaca attaaaacca
      421 tactatcttg aatcatcatc aaatttaacg catgtgcgtc aagttgtttc actttaaaga
      481 cttcattatt aagaccgacc ttagactgct ttttaaaaat aggtacattc gtaatcatac
      541 ctatactttg attgtctaag tgtaatttgt tttggtgata tcgattataa tcttgtgcag
      601 taaaagctgt aatcattact gattgtcttg aaaatatcgc gccttcatta ccaatgaagt
      661 ctattggaat atcatcacta gcctgtttta acttgtaata agcacgattc ttttctaaag
      721 aagctctaaa taatgtcgtt tcatatactt tgaagtcttt tgcattaacc gaaccctgta
      781 tatcacgttt aatattctca attgtttgtt gacgttctat ttgtgactta gaattgtcgc
      841 taaatgacaa atcataatca ttcgtaatca atttcgtaat ggtatgattc atatcacgat
      901 aggttgttaa tgtcattgtg agcgttacaa tcaaaaatgt acacagcaaa gtgattgttg
      961 caagactgac tgcattcatt ttaagacgta cacgcatccc aactaccaca aaaaaatacc
     1021 gtggatgata gtaaactttt gatacctgct gcaatatact aataattatt tcacttatac
     1081 ctacaaaaaa tgcataagta ccgataataa ctaatcctat gacaatccat attttaaaaa
     1141 aggcaccaaa cgtcgtatct tgttgtaatg caataaagta acctaaatat agtgctgcgc
     1201 ttcctataac aattaaaacg taacgcaacc atcgtgatat gacactagaa tcggaacgat
     1261 gttgataagt tatcggccgt tgaaaattaa tactaaacaa gttgaatata agtagcatgc
     1321 ccattaatac agcaatgata atcaaagtaa taaacatcgc tacagagtca aatggataat
     1381 accttaacgt cgcaacttca ctgcccatta ttttctgtat aaacaagaaa aatatcgcac
     1441 caagtaagta tccgccggca atactaacga ctgaaattat cacaaattgt aagatagttt
     1501 ccatcactac aattaaacgc atacttttct tggtcatgcc caatgtcata aaaatgctaa
     1561 actcttttcg tctttgtgac atcataaagt gatttgcata gaaaataaaa ataaaagtta
     1621 aaagcgccat aaaaaaatta gctatgataa taaatggtac taggaagtca ttcttctgtt
     1681 ttatgtagct atttaaccca attgacacaa gcgtatattc aatcatgaat agtacactta
     1741 ccgctaaaat gaatggaatg attacaagtc gctgcgtaat aaattgacgt cgcactatgt
     1801 gccataacaa tcttaacttc atatattgac acttcctcca ttcacaagtg ctaagctatc
     1861 tgttattcgt tgttgaaaag ctaattgact ttcttcacca cgatatattt catgatatag
     1921 acgcccatct ttaataaaaa tgactcgctc tgcataagac gcatcgatat ttgaatgagt
     1981 taccattaaa attgtctgtt ccaattgatg aatctcttga aataacatca tcaatgcctt
     2041 tgatgttttt gaatcaagtg cacctgtagg ttcatcggct agtaaaatcg tcggcttagt
     2101 aactaatgca cgtgcaatgg caatgcgttg cttctgaccc ccagagattt cagaaggata
     2161 cttgtttaag aatccctcta aatgtaattg tactgccaac tgatgtaccc tttgttctat
     2221 atcttttcgt ttagcaccag ctaaaattaa tggcatcatt atattttctt tattcgtcat
     2281 tgttggtaaa agattaaaat cttgaaaaac aaaacctacc atttgttgac gatacaatgc
     2341 tttactttta tttttcatat tatttaaatg tgcgccatcc acaataatgt caccttcagt
     2401 tagtccatca aaagaagcaa ttaaatttag tagtgtagac ttccctgacc cagactcacc
     2461 cataattgca acaaattcac cagctccaac tgataaattc atttgattaa gtgctgtcgt
     2521 agcattcaaa cctttaccat aaaccttttt tacatgtttc acttctagca acatggcatg
     2581 cacctcattt ctttgttagt tctatattaa aacaaaaaaa tactaataac atttaaatta
     2641 gtaaaaaaat gttactagta tacttacaat attgaaagtt tttattcatc tggaaattga
     2701 atcgtaaatg ttgtaccctc attttgttta gatacgactg aaacaggatg gtttgtgtgt
     2761 gttgaaattt gttttacgat aaataaacca atcccacttg agttactttg gcgctggcca
     2821 ttataacctg aatagccctt atcaaatatt ttaggcaagt ccgcttcact aataccgata
     2881 ccattatctt ttacgtgtaa ttgattggat tgctcatcaa attcaatcca tatatcttta
     2941 cctctcgcat acttaagtgc attatttatt aattgttcta tcattaaaga ggtccatcta
     3001 acgtcagtta atacttcgtg atgacaaggt tcataatgga tttttgtttt ttgatcaata
     3061 aactgtattg aatatttcat aataattggg cgaatgatat tattaatcga aattttagtg
     3121 acagaaatat cagaagtttc atttaataac tttaaataac taagtgctaa acttgtatag
     3181 ttatcaattt gaataacctc ttgacgaaca cgattaacaa cattaggctc atctctttca
     3241 agtaacagtt gtgctgcagt aatgggtgtt ttcatttgat gtacccatgt taaaaagtag
     3301 ctctctacat catttttata ttcaatttgt tcatttttaa gctgatacaa cgcattttct
     3361 aagttttcaa tttgttgttg ttggcttata gtttttacaa aacttaaata tttaatacct
     3421 atgaatatga gtaataataa taaaataacg ccaagtgcta gtaaatatgc ttctaatggc
     3481 aaatgataca ggtaaaacat taagccaaac aaagcaaaaa taactatgac tattgctatt
     3541 tcctgagtaa tacttttaag aaaggtcatt gcattcaccc tatactttat atccgacatt
     3601 tttctttgta atgataaaat cattaacgcc aatagtattt aattttttac gcaggcgcgt
     3661 catgttaaca gctaatgtgt tatcatctat gaagttttct gattcccaac atttttcaat
     3721 taaagcagtt ctacttacat atttatcttc attttgaaat aataacttta atatttgtaa
     3781 ttcagtcaaa gatagctgta tgttttgttc ttgatacacg acttttgctt catctaatat
     3841 taatgtacaa cctttaaccg tcaatgaatc attagctact gacaagtcat aagttcgtct
     3901 caataatgct tgaattttgg caatcgttaa tgacaagtta aatggctttt cgataaaatc
     3961 atctcccccc atttgtattg ccataatttg gtccatatta tcaatacggg aactaataaa
     4021 tataattggc acattagatg tttttcggat ttcttgacac caatgaaaac catttaacgt
     4081 tggcaaatta atatctagca atacaagctg aggttgattt tggttaaaga tagacagtat
     4141 atcatcaaat tgttcaacga caataacacc gtaattccat tttttaagtt cagatgctaa
     4201 actctctgct ataacaaaat catcttcaac aattaatatt ttcatctata atcttcttcc
     4261 ttcaattgaa ttatcctttg ttatcttttt gttctttatc ctgctgcaat gacggctctt
     4321 gatttgaact ttccgtgtca tttgtatttt gatttagtgt atttgctttt tctttctcga
     4381 gttgcttttt cttcgtacgt ttacgctctt ctaaaataga caacactata aagaatatga
     4441 ttaataatgg agataacatt actagtatca tttttccacc tcaaattata tttaattcta
     4501 tattttcaca cgcttctatt ctaacgcttt ctataacaaa ttgtcatata tattcatttt
     4561 ttgttattct aaatcactct gtttacatat ttttaactaa aactacataa ttgctcaaaa
     4621 ttcctctttt acactgaaga catagtcata tactcaggag gatgtcttat gttatcagtt
     4681 aaaaaagcca caaaatttgc agttgctaac cttattatcg tgtcaacggt ttttagttct
     4741 gtcacacctg caattgcgca atccgataaa agttctaaag atgaaagttt cgcggttggt
     4801 aatactaaaa atccaaaaaa tgttatcttt ttagttggcg atggcatggg tccatcattt
     4861 aacacagctt atcgttatta taaaaatgat ccgcatgcta aagaaatgac ccctactgct
     4921 tttgattcat atttaaaagg cacaaatcgt acatattcaa atgacccaaa acagaatatt
     4981 actgattctg cagctggcgg aacagccttt agttctggtc ataagacata taatggtgcc
     5041 atcggtgttg acagtaacaa gcagaaagta aagacggtgc ttgaacgtgc aaaagaaaaa
     5101 ggtaagtcga ctggtcttgt ttcaacagct gaattaacag atgcaacacc agcagcatat
     5161 gctgcacacg tgacttctag agatgataaa aacgaaatcg ctaaacaatt ttataaagat
     5221 aaaatcaacg gaaagcataa agttgatgtt ttattaggcg gcggcgctaa atatttcggt
     5281 aaaagtaatg gcaatttaga taaaaaattc aaaaaagatg gttacgacct tgccacgaac
     5341 agtaaggagt tatcaaaatc tgataaagat aaagttcttg gtttgtttgc agataaaaat
     5401 atgccacttg caatagatgc ttctaaagac gaaccatcat tagctgatat gcaacaaagt
     5461 gcacttagta agcttgagcg caataaaaaa ggattctttt taatggtcga aggtgcttct
     5521 atagataaat cagcgcattc caacgatatt acaggcgtta tgtctgaaat ggaaggtttt
     5581 gaaaaagcat tcgatgacgc cattcaatat gctaagaaac ataaagatac acttgtcgtt
     5641 gcaacagcag atcactctac tggtggtcta acaattggta aagataaagg atacgaatgg
     5701 aatcctcaac cgattaaatc tatgaaacac tctggatcat atatgacaga aaaaatggct
     5761 aaaggtgaag accctgaaaa agttattaat gaaggttatg gatttgaatt ttcacaagat
     5821 gatatgaaaa aggttaaaaa agagaataaa aagctaaaaa gactacttaa aaaagaaaaa
     5881 gacgaaaaat ccgcagaagt agaaacgcaa acaaaagccc tacaacacgc tattcagaag
     5941 ccagtaaatg ataaatctta tacagggtgg acttccgatg gacatacagg cgaggatgtt
     6001 aatacctacg cttacggacc tagatcagaa tcatttagtg gtaatatcga aaatacagaa
     6061 agcgcaaaaa taatatttga tatattcaag taaagcataa aaaaattccc gagcactata
     6121 acaaggtgtt cgggaatttt ttatggttta atttagtact caatgttagg tgcttggtta
     6181 acacgttctt ggtatttttt aaatgcgaag tataaaccgc caactaatgt taaaataact
     6241 gctaattttt tcatcattca aactcctttc taagatgtgt tgaaactgag ttcaattaaa
     6301 ttatatgttt ttattataca ctttttgaca tattttttaa atttaagaat gcgaagattt
     6361 ttaacatttc tgatgctagc tttctttttc atctttcaaa tgcttaataa aatcaaagcc
     6421 aggtcgccct ttagccaatg cttccatcac atcataccaa tccgaaccga cttgttgata
     6481 tatattttgc aataattcat cttgcgcctt gttcaattca gcaatgagtt gatcgccttt
     6541 atccgtcgaa tataattttt tgacacgtcg atcagtctct aaaacttttt caataatgag
     6601 accttgctct tttaattttt gaagtgttgc atgtgatcct tgtttagaaa tttctaatat
     6661 ttctagtaat gatttaatag taataccagg taatttattg ataaaaaaca aaaaacgatg
     6721 atgctgacga ctcataccat acttctctat aatttcatca gcggtagtaa taaaggtttt
     6781 atatgcaaaa taaaacaaca tatgctcata atcgttttta ttggtcgaca tgatgatgac
     6841 tcctttcaaa gcgttaacct cattataata aaatttataa tgtaagtcat acaaatgatg
     6901 ataaaactat aagctaaatc taagtttcac gagaaacatt tttgtgtatc agcaaattgc
     6961 gccaatcata ttaatacaaa aataatttaa acacttagaa atgttagtcg tgcgtaaata
     7021 gtttgtatct ctcactctgt gtatttaatg cagccacata ttttccaaga aataattaat
     7081 cattcaccat ccatgttata gcacgcttaa tcgcttgatc ccaatatgca taatcatgat
     7141 ctcctggtcc atcttcaaat tgataaggaa catttatgcg tgataaataa tcgataaaat
     7201 ctaagttgtc ttgatataaa aagtcttgtt taccacacat aatgagcaat tttggaattt
     7261 gtttatcttc agctacagct ttgtctagca agtaatacgg atcatgttca gttcctttaa
     7321 cacttgaaag attgccaatt atggcctctt ttgaaaaatc attccactct agatccatta
     7381 aattttgcgc ttcaaacaca gcagataatg gtacagcttt ggcaaattta tccccttgtg
     7441 ttaatgcaaa tttaattgtg ccatatcctc ccatagagtg acctgctata aaattgtcat
     7501 cacgcttttt ggaaagtgga aatatttgat gaacataatc atacacttcc aaaatataat
     7561 cataatagct atgaccatat gccatgttag catatgcgct atgatccaca ttgggcataa
     7621 tcacagctaa tttgtgttca ttcgcatacc tttctatgct tgtatatctc atatatg
//
LOCUS       DANRDI010000122         7628 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.122, whole genome shotgun sequence.
ACCESSION   DANRDI010000122 DANRDI010000000
VERSION     DANRDI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.122"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            53..141
                     /locus_tag="PZ377_002078"
     tRNA            53..141
                     /locus_tag="PZ377_002078"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ser,seq:gga)
     gene            complement(715..923)
                     /locus_tag="PZ377_002079"
                     /pseudo
     CDS             complement(715..923)
                     /locus_tag="PZ377_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1384..2171)
                     /locus_tag="PZ377_002080"
                     /pseudo
     CDS             complement(1384..2171)
                     /locus_tag="PZ377_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            complement(2184..2723)
                     /locus_tag="PZ377_002081"
     CDS             complement(2184..2723)
                     /locus_tag="PZ377_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358943.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            3522..4457
                     /gene="lukE"
                     /locus_tag="PZ377_002082"
     CDS             3522..4457
                     /gene="lukE"
                     /locus_tag="PZ377_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HDM6358944.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            4459..5442
                     /gene="lukD"
                     /locus_tag="PZ377_002083"
     CDS             4459..5442
                     /gene="lukD"
                     /locus_tag="PZ377_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HDM6358945.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            5684..5827
                     /locus_tag="PZ377_002084"
     CDS             5684..5827
                     /locus_tag="PZ377_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDM6358946.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
     gene            6564..6707
                     /locus_tag="PZ377_002085"
     CDS             6564..6707
                     /locus_tag="PZ377_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDM6358947.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            6772..>7628
                     /locus_tag="PZ377_002086"
     CDS             6772..>7628
                     /locus_tag="PZ377_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HDM6358948.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVK
                     VYIHNLVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAYEKTE
                     IGFGEELYK"
BASE COUNT         2787 a          992 c         1150 g         2699 t
ORIGIN      
        1 caagcagaag tgaaatatcg cttctgtttt tttattacat atttattgtt gaggaaggtt
       61 gtccgagctg gccgaaggag cacgcctgga aagtgtgtag gcgtcacaag cgtctcaagg
      121 gttcgaatcc cttaccttcc gtaaaggcgc ttaaattggt tttacccatt ttaagcgcta
      181 tttttatttt ggactcaatc ccttgatata tctgcatttg agctattatc ctcattttta
      241 cacttcttat ttatttatat ccatttaaaa ttttttagcc acaatgtgac taatttttga
      301 tgaataatcc taattttagt cacaagattt tgaagtttag tcacaaaaca aatcattcag
      361 atttttttct ataaatttag tttcattatc ttcaaattct ttaatcaaat gtgaatatgt
      421 tttaagcgtt gtatgtacat ctgcatgacc taatcgtttt gaaatgtaat gtttagctgt
      481 tacttaaaga aaatcaaata ttatatatac gcttgaaaaa ataaaataat aatattataa
      541 tataatcaat cggcatctac gtgcggatta taaagtaaag cgtagcataa ccaggagtta
      601 gtcgattttt aataaattcg actaaattat taataaaggc taaagtaatt agtttacttt
      661 agcctttatt gcatttgtaa tttatagaac tgcgaacagg tgactactgt taatttatgt
      721 gattaaagtt tcaatgatat ttgatttatt agaccatttc aaaatcgcaa ggttctcatg
      781 ttctgctaca gtttgcggtc tttggaagat atatcattta ctattattaa catgatatat
      841 tctaatttgt ttgtggatgg gctgtttggt ttgatatcgc ggtaaatata agcatcatta
      901 gttatattgt tgaaatctaa gttgaattta ttatcataca gcaatgtgcc aattagggac
      961 atttttaatt ttgaactcta cattttgttg agagtatctg gttatatttg caaaatggta
     1021 tgaacattta tcatcactta ttaagttgtc ttgatgattg aatgacccag tatttattca
     1081 gttcgagaag attgagagtg ttaaatcgta ttcgagtata atttcgattc tagtcatacc
     1141 attttcataa agtttagcca tttgtaactt aaactatgaa ctaaataatc ttctgactct
     1201 tgtcaaaata aaatcgccta cccaaatagt ttaacaatat ctattctcat agaatttgtt
     1261 caactaagtg tagacgattc tgcattatca ataaggctat tcaatttgct atcttaagct
     1321 gtttcgaaat tttcaaattc gtttcattta atataaacat tgatattatc acgtctatat
     1381 atattaaatc aataaaccta tcttttcatc taattcgcta taatctattg tactaatata
     1441 gttttcatga agaagatttt tattatgttc atttatttct ttttttgagt catttaaaat
     1501 aaaaataaac tcattttgcc tgtgtctttt tatttttttg cttgcatagc atccgttatt
     1561 ttcgacttaa taatcataga attattaggg ttagaaatag cattgataaa tttttctttt
     1621 tgatttttct tagcgctaat aaaaaagtcg aaattatgaa caacaccact gcttcctata
     1681 ataggtaaat ctcttccgta ataaatgtta tgttcatcca atttgtttgc aacatcttct
     1741 gtaaatatgt tctgtgactt aggattagaa agtaagtaca tatcattaac aaatataagg
     1801 cactgtaata aattatgttt cgatttatta aaatttttaa agttagtttg aacaaaaatt
     1861 tcgtgagttt tatcgttata tttaataccg taagctgaaa ggtgctcttc aaaaatcttt
     1921 ttacgttttt ttgatttatt taaagaaata ccattatttt ctaaatcaaa tatagtatag
     1981 ccgtcatctg tcagtgtaat catgtctctg gactgatcgt ataaagcgta aattattaaa
     2041 ttatctaaag aattatcttt aaaaggagta tctattctta ctacattgtt tgataaatta
     2101 gaaaactcta aggtctgatt ataccaatca agatattctt tttttaattt tttagcatca
     2161 aatgtagtca tattatccct cctttattca aggtctttta tattattata ctctaaaaac
     2221 gacatataca cattatacaa gttatagatg tcggggaaat ctttcaagtt aatttcataa
     2281 gcaaacctat cctttttatc atacttatca ttatatatgt gaatatgact tttcggagcg
     2341 attgttccat ctggattatc gtgagtacca ccgttaatat ctattctaac tagggtgtga
     2401 taggtatctt tgaaaatgat tgaaattgaa aacctatctt tttctatgtt ccctcttttt
     2461 attgttaata agtactttat tcctaactta aaacttatta tttcttgcct ttcatttatt
     2521 cgactagcaa ctattgtttt taattcattt acatcgatat gagtttctgg atattttaat
     2581 tcatttatta ataattttac gtgctcgtcg tcaagactat ttaaatcaag tttagccatg
     2641 tctgttcctg tataggcatt aatttttaat actttcttat ttgaaaaaag caaaaaagat
     2701 aggtgagatg catacgcaac caaaagtgta taatatcaat ttactacctc gcaatataat
     2761 tttatacaat gtataataag atttaaagat gtagctcatc aacattttca taggggattt
     2821 ttaaaattaa gtattgaaaa ataaaatatt tatattataa tataaataag tcaacgacca
     2881 tgcgtggaca gtaaaacgaa gtacatgcga atgtagtagc cgtttactat gagactaggc
     2941 attataattg aatatcatag tataaattgc catgagagta gaagcttcag tttatgaaga
     3001 cttcgaaatc atggtttttt atttttaaag tgaaatttta gttcatagta aatatatata
     3061 tgtttttaat tagactaaat catagcatta aattttttta gatgaagtaa gcgatattac
     3121 gaaatgatat atagaacatt tcagttataa cagtatacga agtaatcatc ttcgtttaac
     3181 ggacaatagt taataatcag ttaattttaa attcaattat agattatttg tgataataca
     3241 aacattattt ttgaagattt gattaaagtc gtttttattt ttacagagaa aagattgagt
     3301 atcggtaatt ttatcatatt gttcgaatat aaagggaact atttaaaggt aatgaaggtt
     3361 gcgccaaaat gaccggtttt cagcagcata aatgaataaa ttgtgtcatt tttaatattt
     3421 tatagcattt tattttttaa tgaacagtta attttataaa taagtgaaat aatctagaat
     3481 aaatttgtat taaaaattta tatagaaaga aagtgaaact tatgtttaag aaaaaaatgt
     3541 tagctgcaac tttgtcagta ggactgattg cacctttagc atctccgatt caagaatcta
     3601 gagcaaatac taatattgaa aatattggtg atggtgctga agtaatcaaa cgtacggagg
     3661 atgtaagtag taagaaatgg ggcgttactc aaaatgtcca attcgacttt gtaaaagata
     3721 aaaaatataa caaagacgct ttaattgtta aaatgcaagg ttttattaat tccagaactt
     3781 cattttcaga tgtgaagggt agtggatatg aattaactaa acgaatgatt tggccattcc
     3841 aatataatat aggactgacg actaaagatc caaatgttag cttaatcaat taccttccta
     3901 aaaacaaaat agaaactact gatgttggtc aaacattagg atataacatt ggaggtaatt
     3961 tccagtcagc accatctata ggtggcaatg gctcatttaa ttattctaaa acaattagtt
     4021 atacccaaaa gagttatgtc agtgaagtag acaagcaaaa ctcaaaatct gttaaatggg
     4081 gtgttaaagc aaacgaattt gttacgcctg atggaaaaaa atctgcgcat gatagatatt
     4141 tattcgtaca aagtccaaat ggtccaacag gttcagcaag agaatatttt gctcctgata
     4201 atcaattgcc acctttagtt caaagtggct ttaatccatc gtttatcact acactatcac
     4261 atgaaaaagg ttcaagtgat acgagtgaat ttgaaatttc atatggtaga aacttagata
     4321 ttacatatgc gactttattc cctagaactg gtatttacgc agaaagaaag cataatgcat
     4381 ttgtaaatag aaactttgta gttagatatg aagttaattg gaaaacacac gaaattaaag
     4441 tgaaaggaca taattaatat gaaaatgaaa aaattagtca aatcatcagt tgcttcatca
     4501 attgcactgc ttttgctatc gaatacagtt gatgcagctc aacatatcac acctgtaagc
     4561 gagaaaaaag tagatgacaa aatcacttta tacaaaacaa cagcaacatc tgataatgat
     4621 aaattgaata tttctcaaat tttaacgttt aatttcatta aggataaaag ttatgacaaa
     4681 gatacgttag tacttaaggc agccggaaac attaattcag gttataaaaa gcctaatcca
     4741 aaagattaca attactcaca gttttattgg ggcggtaagt ataatgtttc ggttagttca
     4801 gaatcaaatg atgctgtaaa tgttgttgac tatgcaccta aaaatcaaaa tgaagaattc
     4861 caagttcaac aaacattagg ttattcttat ggcggagata ttaatatatc taatggctta
     4921 tcaggtggat taaatggatc aaaatcattt tcagaaacga taaattataa acaagaaagt
     4981 tacagaacta cgattgatag aaaaacaaat cataaatcaa ttggctgggg tgttgaggcg
     5041 cacaaaatta tgaataatgg ttggggacca tatggtagag atagttatga cccaacatat
     5101 ggtaatgaac tgtttttagg cggtagacaa agtagttcaa atgctggtca aaatttcttg
     5161 ccaacacatc aaatgccttt attggcgcgt ggtaacttta acccagaatt tataagcgta
     5221 ctttctcata aacaaaatga tacaaaaaaa tctaaaatca aagtaactta ccaaagagaa
     5281 atggatagat atactaatca atggaatcga ctacactggg ttggtaataa ctacaaaaat
     5341 caaaatacag taacgtttac atctacttat gaagttgact ggcaaaacca tactgttaaa
     5401 ttaatcggta cggattctaa agaaactaat cctggagtat aactgttaga aatagaggaa
     5461 gttaaacccg aaatttaaga gttaaacaaa aaatattcac aacatattaa aaaaagaagt
     5521 tacattattt gtttaaacta acaatagtac cataaaatta atgacgattt acaaattaat
     5581 aacaaataat tattattaaa ttcattgatt tattgtgtgg taatgattaa tatgacaatt
     5641 gaagtgggtc ttaacttcaa cttttaaata gggggtttta tctttggaaa aagttcttga
     5701 tttagacgtg caagttaaag gaaataacaa cactaatgat tcagcgggtg acgaaagaat
     5761 aactagccat cttttttgta gctttggttg tgaaaagacg ggtagtttta acagcttctg
     5821 ttgttaagga tacagcaaat agagcctgaa agggctctat ttgtcttttt aaatatatta
     5881 agtgctaaaa aacggaaaca gtttattaat cgccaggtat ttttataatt agccttctat
     5941 attatcagtt gagtgtttca ataaattctg actttggtga tggaaagtta tacttttagt
     6001 tgtaatatag ttttcatgac ttcctgttaa ataagttagg attatgtaat tagcctctat
     6061 ctttacatat aagtaatgac aagctttggt tattatattg accctcttag aacatgtcat
     6121 ttaagaaaat aatcaagtca ttgaaattat atgtttatat cctcatcttt aaaataactt
     6181 caattattat ttgctaaatt ttgtataaat agaacaaaac tgcataaata tctattaatt
     6241 aatctcaagc agccatctcc aaattcgtaa tagtattgat agataaaaat atctaagtat
     6301 ttgtataaat gaattgatta atctattaaa aaattcatca ataaatttaa aattatgaac
     6361 ttagatgcaa aattattagt gaaaataaat agtaataaaa aaacacaaaa atctaattat
     6421 caaaatagta ttactaaatt aataagtgtt tacaaaaaat tcacaaaata taattatata
     6481 atttattgat ttgttgtgtg aaaagggata atatgacaat tgaagtaggc cttaacttca
     6541 actaataata ggaggtttta tctatggaaa aagttcttga tttagacgtg caagttaaag
     6601 caaacaataa ctcaaatgat tcagcaggtg acgaacgtat tacaagtcat agtttatgta
     6661 ctcctggttg tgctaagact ggtagtttta atagcttctg ctgttaagat tacagcaaat
     6721 agagcctgaa agggctctat ttgtctattc aaatatgtga ggtgcttaca aatgaacact
     6781 atttacgaac cgtcaagtat ttgtatgatt agaacacctt tattatcagt tgaatttttc
     6841 aatttatttt taaatactga acaaattaaa tatagcgact tacaattaaa tgctcagatg
     6901 aaagaatcaa tcctaacaac aacatttaac ttatattgta ctttacaaga aataaatttt
     6961 gatggcgata acaagaaggt tagagatgct aaagaaagtt tattaaaata tttaattaga
     7021 atgtcaacac gtccaactcc atttggatta ttaagtggta ttaatttggg gcattttgta
     7081 aatgaaccaa cacgtcttaa agttggaaat agtattcaaa aatatgtgaa agtcgatggc
     7141 gaatggttgt ataaactggt tagttatatt gagagtattg atgaatatta tcaaaatttg
     7201 aaagttattt ggaatagtaa agcacatatt atcaatgatc gaatatattt aaatgaacaa
     7261 agtgctattt atttaaacaa caataaagat acatcttttt ccattaaaaa tagcgaatta
     7321 ctcgtattca tcaaaaccac tgtgacgaat aataatatta ctttttcaaa tttagctgaa
     7381 aagataaatc aagaatttga aattaatgat attactaaag tgaaagtata tatccataat
     7441 cttgtctcaa aggaaattat atattcaaca atcagaccac cgttatcata tagcgataat
     7501 ttaaattata ttttaaataa actttcttta cataatgatg attttgtgaa aaaaattaga
     7561 gaaatccaga aattgatatt agcttatgaa aaaactgaaa ttggatttgg cgaggaatta
     7621 tataaaga
//
LOCUS       DANRDI010000123         7566 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.123, whole genome shotgun sequence.
ACCESSION   DANRDI010000123 DANRDI010000000
VERSION     DANRDI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.123"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..559)
                     /locus_tag="PZ377_002087"
     CDS             complement(<1..559)
                     /locus_tag="PZ377_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDM6358949.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINT"
     gene            complement(806..2176)
                     /locus_tag="PZ377_002088"
     CDS             complement(806..2176)
                     /locus_tag="PZ377_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDM6358950.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(2498..2593)
                     /locus_tag="PZ377_002089"
     CDS             complement(2498..2593)
                     /locus_tag="PZ377_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358951.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(2669..3274)
                     /locus_tag="PZ377_002090"
     CDS             complement(2669..3274)
                     /locus_tag="PZ377_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDM6358952.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(3337..5217)
                     /locus_tag="PZ377_002091"
     CDS             complement(3337..5217)
                     /locus_tag="PZ377_002091"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDM6358953.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            5412..5504
                     /locus_tag="PZ377_002092"
     CDS             5412..5504
                     /locus_tag="PZ377_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358954.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(5612..6109)
                     /locus_tag="PZ377_002093"
     CDS             complement(5612..6109)
                     /locus_tag="PZ377_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDM6358955.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(6119..6379)
                     /locus_tag="PZ377_002094"
     CDS             complement(6119..6379)
                     /locus_tag="PZ377_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358956.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(6761..6874)
                     /locus_tag="PZ377_002095"
     CDS             complement(6761..6874)
                     /locus_tag="PZ377_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358957.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
BASE COUNT         2334 a         1398 c         1035 g         2799 t
ORIGIN      
        1 aggtattaat ttcatattct aaactttcaa tcgcatcatt gatgtcttta gtgacttgtt
       61 gatcaacata acgatcaatt tcagattctt tgacaaattg ctttgcgata ttcctatgtt
      121 gttcttcatt atgtcgtgca tatgtactaa gtaattcgtc aacgcggtca accgtacagc
      181 caccatattg actgctagaa acattggcta taatttgtac aagctgcgct gatgcagttt
      241 gtattgattt tggtgaagtt acattcgcgt tgcctatttc aaatccatta tgtagcatat
      301 ttttagcatc tattaaacaa cagttagtta acggttggaa tggatgataa tctaagtcgt
      361 gaaaatgtat gtcccctctt tgatgtgcat ctgcaacgtg ctttggtaat aaatgattta
      421 atgcgtaaga tttagaaacg atacctgctg ttaaatctct cattgttgaa aatgtatcac
      481 tatctttgtt agcattttcg ttaacaatag caggatcttt agaaatcaaa cccgttaatg
      541 cttcttcgat ttgattcatt ttaccccttc ttcctatata ttgtgtctac tcattacgtt
      601 ataccactat atatagtatt ttcaaatttt tattagtctg ttaactaaaa cgtcacaaag
      661 ttattaacat ttagtgaaat tcatatttct acacaattat ttcattaatt tttaaaattg
      721 tttaaagatt cgtccgtcat ttcgacatgt ttatgcaact attatttcat tttgcaacgt
      781 gaccatagtt tctatttttt catacttaaa tcgtcacaat gcccatcaac attgcaatta
      841 ctaacataac gatagcgaat ccccaaatcc aaaagaatgc atacttaata tacgtgccca
      901 tgtttgcctc tgctaaacca attgccaacc ataaggctgg tgaaaatggg ctgacaaatg
      961 tacctataat attccctatg accattgaat aagctgttga tacagacggt acaccaaatt
     1021 gccctgctgt ttgttcgaca atcggtaaca cagcaaaata ataagcgtct gtgctagtta
     1081 gtaaatctaa tggtacgcca agtaaaccta caataatatg caagtatggt cctacttctg
     1141 caggaatcac tttgattaaa ttggtcgcaa tcgctttaag cataccggtt tcatttagta
     1201 cacctaaaaa catacctgct gcaataatca ctgcagccat cattaatgca ttcggcgcat
     1261 gcgctcttaa tcgttccatt tgttcatcca ctgatttaaa attaataaca agtgccaacg
     1321 aaacacctat catgaatgca aattcaggtg gcgcaatatt tattaacatc gatagaataa
     1381 cagctaaagt taaagctgta ttcacccatt ttatccatga ttttgttctt gcacgtcctt
     1441 ttacaggaaa ccttacatct tgatctcgtt catatacttc aactaattta tgtacatcta
     1501 tatcttgtgt ttgcggtaat tcatttctct ctattgcttt tttgatacgt ttctgttctt
     1561 taaatccaag atataccgca aacaacataa caagaatgaa acctattatt tgaataggta
     1621 ttaatccata ccataattca ttgacacttt tggcttttaa cactgcagct acacgagcca
     1681 ttggacctcc ccaaggtacc atgttcataa tcgccgcgct taatgctaat agtaaaatca
     1741 ataaatattt attcatattt aacgctttat ataaaggtaa taatgcagga atagaaagca
     1801 aaaatgttac cgcaccggcc ccatctaatt gggctattgt gccaattaaa gctgtcattg
     1861 cacagacaat gacgacattg cctcgtgtca ttaatattaa gcgtttgaca agcggcttga
     1921 ataaaccact atcgttcatg atgccaaaga aaataatggc aaagataaac ataataacaa
     1981 cgttgatgac ttgatctaac cctttagcaa aaaatccaac caaatctgtc acactatatc
     2041 ctaaaatcat tgcccctaag caaggtatga ttgtcatacc tacaactgga tttatctttt
     2101 tggcaatgag taaacctaca attgaaataa taataatgag ccccattact gttaaccaca
     2161 tattatcact attcatatta ttttctccct ccacttttaa tacaatttac ataatccccc
     2221 tctttaactt aaatattata attaatataa ttttttatat ttaagttatc tcattttatc
     2281 aaaaaaatct tttttgcgta actatcaata gtgaagtttg attttcctac ttgataacat
     2341 ggcatgacta atccttgtat atcctgtcct gtatttcatt ttgatctatc atttttatta
     2401 ttgctttcta ttgcacaaaa aatctggcta aaggtcgcta tgatagtaac gacttcaacc
     2461 agatttcaat ttatccacct attttattaa gtttgttcta cgtatctttt tctctattgt
     2521 aatattcata tttaaaacgc atatcattat cgcttaaatc cttcattatt agactattca
     2581 caaagccgcc catgtcttaa tttcaatgat taagcgttaa agttaaaata agaagactta
     2641 ccttagacgg ttagacggtc cgctattatt atcttacatc caaccacgct aaaaattgag
     2701 cttgtttgtc atgatttaaa tattcttgtg acacaatttg tgacagtaac tgttgctttt
     2761 cgttatatgt tatatcaagt acttttattt tctgtcggca agtatataaa aagtcgatat
     2821 acgaactgta tgatggtgga tataacgcct caagctctgc cataattgat tttgtcaact
     2881 taggactcgc accatcagtt gatacactga tagttagctt gtcgcggtgt agtgcacttg
     2941 gaaatacaac attgccattt gatgcatctc caacattatt aaaaagggca tgctcaggta
     3001 aggctttttt taccgcttca ttgacacgtg gctcattggt tgctgcaata actagatacg
     3061 cgtctacaat atcgcttggt tcaaattctt tttctttcca tatgacgaca ccgttatcta
     3121 ccatattttg aagtttttca gtgattgtcg gactgatgac cgtcatatgt tcaacgtatt
     3181 gatttaatgt ttgtgcccga cgacttgcaa cgacgcctcc accaactatg acgacatttt
     3241 tatttgttaa atctatcatt aatggcatat tcatgttgag caacatcctt tatatctttt
     3301 tacatcatta caccttaaaa tatttaacac caatcgctaa taaacatcac gttgatagcg
     3361 ttgttgttgt ttcatttgtc gcaataataa ctctgcttct tcttgagaaa tatgacgttc
     3421 ttttaccaat acatctttaa tggcttgatg gacatctttc gccatacatt tttcatcgcc
     3481 acaaatatag attgctgcgc cttgctcaat ccattcattg aaacgtttac tttcttccat
     3541 tatacgatgc tgtacatatt ctttgtgttc ttggtctctt gaaaatgcta aatctacgcg
     3601 tgttaagttt ccattttcaa gccattcttc tatttcttct tcatataaaa agtcagaact
     3661 acggtgttga tcaccaaaga acaaccatgt ttttccagtc ataccaagtt cttcacgttc
     3721 ttgtaaatat gctctaaaag gagcaattcc agtacctggt ccaatcataa taaccggtat
     3781 atcttgcttc atcggaaatt tgaagttcgg atttttcttc aaataaattg gtactatatc
     3841 gcctggttta attcgctcag caaaatgaac cgagcataca ccttttctct cacgtccatg
     3901 tgcttgataa cgaaccgtac caacggtaat atgcacttca tctggcgttg ccataaaact
     3961 actagaaatc gaatactctc ttggtggtaa ttttctaagg atttgataca tattctcagg
     4021 ttgtaattct atagttggaa aatctgttat taaatctata aagtcccgat ttataacata
     4081 ttcacgcgcc catgactcat cttgaatacg ttcagataat tcttcattgt caaaatagat
     4141 atctgcattt ttcaataatg gcaatgttaa tttagtaaat tcaaaatgtg atgttagtgc
     4201 ttcaacaata ggaactgtat caccatgatc attaattggc accggagatt gcggatccca
     4261 acctaacatg aatattagtt tttcaaccaa ttcagggtcg ttttgcggta atgctactat
     4321 acaatctcct ggttcatatg attcactaaa atcatcaagt aaaaattcta tatgtcgtgt
     4381 ttctttattt gaatcggtac catttaaatt gatattcgct aatacttctg cttggtatgg
     4441 atttgattta gaatatttct tttctttggc agacttaatt gattcactta tcaccgattc
     4501 actttgaata ccttctgatg tggtatcaat aatattaatg atgtctgcca tccacttttc
     4561 tgcgtcttct tcataatcaa tatcacaatc tacacgctta catatacgct cagcgcctag
     4621 attttctagt aaaacatcta catctttacc ggcttgacag aaaaattcat atgtttgatc
     4681 acctaaagct agtactgaat atctcacatg atttaaatta ggtgcgttat cgtcttctaa
     4741 aaattcaaag aaatcccatg cattatcagg cggttctcct tcaccatgag tagacgtaat
     4801 aataaataaa tcttctaact gcgcgatgtt tgtcgtatca tattcatcca ttgacatcaa
     4861 aacaacttga tgtccgatat cacttaaacg ttctgaaaaa atttcagcta aacgcatggc
     4921 attacctgat tcagaaccat atagcactgt aacatgccgc tgattagctt cgatatgtgg
     4981 ctctttattt tgtaacatat acgttgttga agttgcttgt tcttctgata tttgtgattg
     5041 ttgccacact tctgtctcta attgttgttg attactatca cttgtcgtat cgttactatt
     5101 agctagtaaa aaaccactaa gccactgttg ttggctctct gttaatgttt gcagcagatt
     5161 gttaatctct gtgacttgtt tttctgtaaa tggactatta gatgtattta atttcaaatg
     5221 ttttcacctc aataaacttg aaatagatga ttgactcttt ttttatcgat agtttagtca
     5281 attttttaat atgtatggta taaattaaaa caaacttgag ttgtgtctga tagttatact
     5341 tgcaaatatt tacaaccttc atgttgcttg aatgacatta tcagtaattc agacaaaact
     5401 ttgattatgt cgtgtcagtt attaatgcat acagtaatgc atcgttgtta ttttttattt
     5461 atttactatt tattatgtac caaatcagcc tttcagtaaa ataggtcaaa ttactgattt
     5521 tctaaatata aaatgcctcc taataacata ctattagtac atcattaaga ggctcttgtg
     5581 ttatttgcat actaagcgct caaatttaaa tttaagatga agattcttgc aataatattt
     5641 ctatatttgt cgatatatcc aatggatctt ccattggcaa gaatcgattt acaacatttc
     5701 cttgtcgatc gattataaat tttgtgaaat tccattttat tggggaccca aagattcctg
     5761 gttgttcatt ctttaaatgc gtaaataacg gatgttcatc gttcccgttc acagatattt
     5821 tagctaacac tggaaatgtt acaccaaatt tctcacgact aattttcaag atttcttcat
     5881 tagaacctgg ttgtcgattg tcaaaattat tattcggaaa actcaacact acaaacccac
     5941 gatccttata tttttgaaat aaagtctcta gttttttcaa ttgttcgcta tatatacatt
     6001 ctgttgcagt attaacaact aaaatcactt tacctctaaa tgcttctaat ttataagtta
     6061 agcctttata atcacttact tcgatatcat acacatttct attattcata agacacccct
     6121 acacagcctt ttttatattg aatatgttct ttttagaatg ttctgataaa ataagtgcgc
     6181 gtttacaccg tgaacacaca ttatatagcg tgatacattt ttcgagcaca cgataaataa
     6241 tgttcgagtt tatgttgttg ctcaacctat ccgatttacc gtcttttttc acctgtctta
     6301 ctttattaaa ataatcacat tgtatagctg tattagtcca gctacaatat cttccgttat
     6361 aacccctcat tataatcatc cttattttct atttttaaaa agacaattag accgctcttt
     6421 aaactataga ttaatactta agttaaactc atacatactg ataccatacg ttagatctaa
     6481 caatttaaaa ttcgttataa ctatggatta aagagctgcc caactcatat aatccttaaa
     6541 aacttcacat gtgattgttt attaagccct cctttatctt attaaatatc cttataaccc
     6601 ttttaaaatt aaactgacac actcatacat tgttacacgc gacgataaac gtcatattat
     6661 tgcatatcat taaaacaagt cactttgtat cgtgtgcgac cgaatacata ttaaatcatc
     6721 aataatgatt attcaattat taaaaataaa aacgaacatt ctatatagag tttctactta
     6781 ttattttttc agcattgtct acattactta acattcgtgt ttgtaagtaa tattgaccgc
     6841 caatatttag acactttata agtatgccat tcatcatttt taatcatatt atttttctta
     6901 ttgatatttc tattcatata tttctgattg tatatttaga tataataatt tgttattgga
     6961 aaatatatga aacaaatttg actcatcttt ttcaatatct aaatgacacg atgcatgatt
     7021 ttttaatttt gtaattaact tccttttaaa ttagactgaa ttgtgacaat tttcaaacat
     7081 tttaatgtgt aaatcattgg caaaatatac gaattgcatt taaatcgtca atttacatat
     7141 atttttgcgt agtaatcaat cgttatcatt gttatttatc gttacattat ttcgagtatc
     7201 agtatgtatt tcgggcttcg tttgataacg acatttcttt gtgacatcgc ttcatcagtg
     7261 taacaacaaa tacaatgatt tcgtgatgtt agttacccat tttatgtgtt gcataaaata
     7321 tgttgttata aaacatttta aatcatttta tataaacaat ctatatattt ttggcatttc
     7381 caaaatatca cttgttatat taaaaaccga caagacattt tatcttatcg gttgaaattt
     7441 gttattgtta tttgtaatgt ttttaggttt ctttttaata taatatattt cagtgaaaat
     7501 acatgattga ttgtgatttc actgaaacat ggttaattgc gttgttgatg aataacttta
     7561 gcataa
//
LOCUS       DANRDI010000124         7545 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.124, whole genome shotgun sequence.
ACCESSION   DANRDI010000124 DANRDI010000000
VERSION     DANRDI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.124"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..213
                     /gene="bshC"
                     /locus_tag="PZ377_002096"
     CDS             <1..213
                     /gene="bshC"
                     /locus_tag="PZ377_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HDM6358958.1"
                     /translation="LKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQILN
                     DFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            357..788
                     /gene="mraZ"
                     /locus_tag="PZ377_002097"
     CDS             357..788
                     /gene="mraZ"
                     /locus_tag="PZ377_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDM6358959.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            804..1739
                     /gene="rsmH"
                     /locus_tag="PZ377_002098"
     CDS             804..1739
                     /gene="rsmH"
                     /locus_tag="PZ377_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDM6358960.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            1753..2154
                     /gene="ftsL"
                     /locus_tag="PZ377_002099"
     CDS             1753..2154
                     /gene="ftsL"
                     /locus_tag="PZ377_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDM6358961.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            2135..4369
                     /locus_tag="PZ377_002100"
     CDS             2135..4369
                     /locus_tag="PZ377_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDM6358962.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            4661..5626
                     /locus_tag="PZ377_002101"
     CDS             4661..5626
                     /locus_tag="PZ377_002101"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDM6358963.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            5628..6977
                     /locus_tag="PZ377_002102"
     CDS             5628..6977
                     /locus_tag="PZ377_002102"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDM6358964.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            6993..>7545
                     /locus_tag="PZ377_002103"
     CDS             6993..>7545
                     /locus_tag="PZ377_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDM6358965.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIA"
BASE COUNT         2789 a         1019 c         1481 g         2256 t
ORIGIN      
        1 ttaaaacgtt atcttttaaa cattgaaaga gaaaacgaca tcagtatgaa gcaatttaga
       61 gaaattcaag aaacactcca tccaatggga ggattacaag aaagaatatg gaatccactt
      121 caaattttga atgattttgg gacagatgtg ttcaagccct ccacctatcc accactttct
      181 tacacttttg atcatattat tataaaacct taatatacca agggtttagc ccgatttatc
      241 ttaatgataa atcgggcatt tttttgtttt ttaaaataaa tttcacaaat tttgtataaa
      301 tagtggtgga tagtggggag atgtggtaaa ttatatataa ggtgaggtga taaaaaatgt
      361 tcatgggaga atacgatcat caattagata caaaaggacg tatgattata ccgtccaagt
      421 ttcgttatga cttaaatgag cgttttatta tcacaagagg ccttgataaa tgtttattcg
      481 gttacactct agacgaatgg caacagattg aagagaaaat gaaaacctta cctatgacaa
      541 aaaaagacgc acgtaagttt atgcgtatgt tcttctctgg tgctgttgaa gtagaacttg
      601 ataagcaagg gcgtattaac atccctcaaa acttgaggaa atacgctaat ttaactaaag
      661 aatgtacagt aatcggtgtt tcaaatcgta ttgagatttg ggatagagaa acttggaatg
      721 atttctatga agaatctgaa gaaagtttcg aagatattgc tgaagattta atagattttg
      781 atttttaaaa tggaggaatt gaagtgtttc atcatatcag cgttatgtta aacgaaacca
      841 ttgattattt aaatgtaaaa gaaaatggtg tgtacattga ctgtacgcta ggtggagcgg
      901 gacatgccct ttatttacta aatcaattaa atgacgacgg aagattaata gcaatcgatc
      961 aagaccaaac tgcaattgat aatgctaaag aggtattaaa ggatcatttg cataaggtga
     1021 cttttgttca tagcaacttc cgtgaattaa ctcaaatatt aaaagactta aacattgaaa
     1081 aagtagatgg aatttattac gacttgggtg tttcaagccc acaactcgac attccagaac
     1141 gaggattcag ttatcaccat gacgcaacat tagacatgcg tatggaccaa acacaagaac
     1201 taacagcata tgaaattgtt aacaattggt catatgaagc gttagtgaag attttttatc
     1261 gctatggcga ggagaaattt tcaaaacaga tagctcgaag aatcgaagca catcgcgaac
     1321 aacaaccaat aacaacaaca ttagaattag ttgacattat aaaagaaggt attcctgcaa
     1381 aagcaagaag aaaaggcgga catcctgcaa aacgagtatt tcaagcacta cgaattgcag
     1441 taaacgatga attgtcagct tttgaagatt caatagaaca agcgattgaa ttagtgaaag
     1501 tagatggcag gatttcggta atcactttcc attctttaga agatcgttta tgtaaacagg
     1561 tgttccaaga atatgaaaaa ggtccagagg taccaagagg attaccagtt ataccagaag
     1621 catatacacc taagttaaag cgtgttaatc gtaaaccgat taccgctaca gaagaagatt
     1681 tagatgacaa taacagagca cgaagcgcga aattacgtgt agctgaaata cttaaataag
     1741 gagcaattta taatggctgt agaaaaagtg taccaaccat atgacgaaca agtttataat
     1801 agtataccga agcaacaacc acaaactaag cccgaaaaga agactgtttc gagaaaagtg
     1861 gttgtacaat taactaaatt tgaaaaagtt ttatacataa ctttgattac tgtaattgct
     1921 atgttaagta tttatatgct atctttaaaa atggatgcgt atgatacgcg aggaaagatt
     1981 gcagatttag attataaaat agataaacaa tcaagtgaaa acagtgcttt acaatctgaa
     2041 atcaaaaaga attcttctta tgaacgcata tacgaaaagg ctaagaaaca ggggatgagc
     2101 cttgagaacg ataatgtaaa ggtagtgcgt agtaatggcg aagcaaaaaa ttaaaattaa
     2161 aaaaaataaa ataggggcag tcctacttgt tggtttattc ggactgctct tttttatatt
     2221 ggttttaaga atttcatata tcatgattac tggacattct aatggtcaag atttagtcat
     2281 gaaggcaaat gaaaagtatt tagttaagaa tgcacaacaa ccagaacgag gaaagatata
     2341 tgatcgtaat ggtaaagtgc tagcagaaga tgtagaaaga tataaacttg ttgcagtaat
     2401 agataaaaag gcgagtgcca attctaaaaa acctaggcat gtagttgata aaaaagagac
     2461 tgcaaagaaa ttatctacag tcattaatat gaagccagag gaaattgaaa agagacttag
     2521 tcaaaagaaa gctttccaaa ttgaatttgg acgcaaagga acaaatttaa cgtatcagga
     2581 caaattgaaa atagagaaaa tgaatttgcc tggtatttct ttattgcctg aaacagaacg
     2641 cttttatcca aatggcaatt ttgcatcaca cttaattggt agagctcaga aaaatccgga
     2701 tactggtgaa cttaaaggtg cacttggagt tgaaaagatt tttgatagtt atttaagtgg
     2761 atctaaagga tcattgagat atattcatga tatttgggga tatatcgcac caaatactaa
     2821 aaaagagaag cagcctaaac gtggtgatga tgtccattta acaatcgatt caaatattca
     2881 agtatttgtt gaagaagctt tagatggcat ggttgaaaga taccagccga aagatttatt
     2941 tgcggttgtc atggatgcca aaactggaga aattttagca tacagtcagc gaccaacatt
     3001 taatcctgaa actggtaaag actttggtaa aaagtgggca aatgaccttt atcaaaacac
     3061 atacgagcct ggatcaacat ttaaatcata tgggttagca gctgctattc aagaaggtgc
     3121 ttttgatcct gataagaaat ataaatctgg acatagagat attatgggtt cacgtatttc
     3181 agactggaat agagtcggtt ggggtgaaat cccaatgtca ctcggattta cttattcatc
     3241 taatacattg atgatgcatt tacaagattt agttggtgca gacaaaatga aatcttggta
     3301 tgaacgattt ggatttggaa aatcaactaa aggtatgttt gatggagaag cacctggtca
     3361 aattggatgg agtaatgagt tgcaacaaaa aacgtcatca tttggtcaat cgacaacagt
     3421 aacacctgtt caaatgttac aagcgcaatc agcgttcttt aatgatggta atatgttaaa
     3481 accatggttt gtgaatagcg ttgaaaatcc tgttagtaaa agacaatttt ataaagggca
     3541 aaaacaaatc gcaggcaaac caataacaaa agatactgct gaaaaagttg aaaagcaatt
     3601 ggatttagtt gtgaatagta agaagagtca cgctgcaaac tatcgtattg atggttatga
     3661 ggtcgaaggt aagactggta cagcacaagt cgctgcacct aatggtggtg gatacgttaa
     3721 aggtccaaac ccatattttg taagttttat gggtgacgcg ccgaagaaaa atcctaaagt
     3781 tattgtatac gctggtatga gcttggcaca aaaaaatgac caagaagctt atgaattagg
     3841 tgttagtaaa gcgtttaaac caataatgga aaatactttg aaatatttaa atgtaggtaa
     3901 atcaaaagat gacacatcta atgcagagta tagtaaagtg ccagatgttg aaggtcaaga
     3961 caaacaaaaa gctattgata atgtgagtgc aaaatcatta gaaccagtta ctattggttc
     4021 tggcacacaa ataaaagcac aatctataaa agcagggaat aaagtcttac ctcatagtaa
     4081 agtactgtta ttaacagatg gagacttaac tatgcctgac atgtcaggat ggacgaaaga
     4141 agatgtcatt gcttttgaaa acctaacaaa tattaaagta aatttaaaag gtagcggttt
     4201 tgtgtcccac caatcaatta gtaagggaca aaaacttact gaaaaagata aaatagacgt
     4261 agaattttca tcagagaatg tagacagcaa ttcgacgaat aattctgatt caaattcaga
     4321 tgataagaag aaatctgaca gtaaaactga caaggataag tcggactaac aagaatggct
     4381 acattaaatt aggatgtatt gtcactattt gtttttacta aagtaatgct gtgatagaaa
     4441 acaacatctt catttaatct ttataaataa gtgttgatag agaattttaa ggtagacgag
     4501 gtcagattaa cttgtttgtt ataggcaaac tttttatact ggcctcttat gccgtaaaaa
     4561 agaaaaatta taaattttga ttacaacttt gtttatttaa ttgaaaaatt gataatatat
     4621 tagtggttaa aaaagtctgg attaagaaga ggagatagtt atgatttttg tatatgcgtt
     4681 attagcgcta gtgattacat ttgttttggt acctgtttta atacctacat taaaaaggat
     4741 gaaatttggt caaagtattc gagaagaagg tccacaaagc catatgaaga agactggtac
     4801 accaacgatg ggtggactaa catttctatt aagtattgtg ataacgtctt tggtggctat
     4861 tatatttgta gatcaagcta atccaatcat actgttatta tttgtgacga ttggttttgg
     4921 gttaattggt tttatagatg attatattat tgttgttaaa aagaataacc aaggtttaac
     4981 aagtaaacag aagtttttgg cgcaaattgg tattgcgatt atattctttg ttttaagtaa
     5041 tgtgtttcat ttggtgaatt tttctacgag catacatatt ccatttacga atgtagcaat
     5101 cccactatca tttgcatatg ttattttcat tgttttttgg caagtaggtt tttctaatgc
     5161 agtaaattta acagatggtt tagatggatt agcaactgga ctgtcaatta tcggatttac
     5221 aatgtatgcc atcatgagct ttgtgttagg agaaacggca attggtattt tctgtatcat
     5281 tatgttgttt gcacttttag gatttttacc atataacatt aaccctgcta aagtgtttat
     5341 gggagataca ggtagcttag ctttaggtgg tatatttgct accatttcaa tcatgcttaa
     5401 tcaggaatta tcattaattt ttataggttt agtattcgta attgaaacat tatctgttat
     5461 gttacaagtc gctagcttta aattgactgg aaagcgtata tttaaaatga gtccgattca
     5521 tcatcatttt gaattgatag gatggagcga atggaaagta gttacagtat tttgggctgt
     5581 tggtctgatt tcaggtttaa tcggtttatg gattggagtg cattaagatg cttaattata
     5641 cagggttaga aaataaaaat gtattagttg tcggtttggc aaaaagtggt tatgaagcag
     5701 ctaaattatt aagtaaatta ggtgcgaatg taactgtcaa tgatggaaaa gacttatcac
     5761 aagatgctca tgcaaaagat ttagaatcta tgggcatttc tgttgtaagt ggaagtcatc
     5821 cattaacgtt gcttgataat aatccaataa ttgttaaaaa tcctggaata ccttatacag
     5881 tatctattat tgatgaagca gtgaaacgag gtttgaaaat tttaacagaa gttgagttaa
     5941 gttatctaat ctctgaagca ccaatcatag ctgtaacggg tacaaatggt aaaacgacag
     6001 ttacttctct aattggagat atgtttaaaa aaagtcgctt aactggaaga ttatccggca
     6061 atattggtta tgttgcatct aaagtagcac aagaagtaaa gcctacagat tatttagtta
     6121 cagagttgtc gtcattccag ttacttggaa tcgaaaagta taaaccacac attgctataa
     6181 ttactaacat ttattcggcg catctagatt accatgaaaa tttagaaaac tatcaaaatg
     6241 ctaaaaagca aatatataaa aatcaaacgg aagaggatta tttgatttgt aattatcatc
     6301 aaagacaagt gatagagtcg gaagaattaa aagctaagac attgtatttc tcaactcaac
     6361 aagaagttga tggtatttat attaaagatg gttttatcgt ttataaaggt gttcgtatta
     6421 ttaacactga agatctagta ttgcctggtg aacataattt agaaaatata ttagcagctg
     6481 tgcttgcttg tattttagct ggtgtaccta ttaaagcaat tattgatagt ttaactacat
     6541 tttcaggaat agagcataga ttgcaatatg ttggtactaa tagaactaat aaatattata
     6601 atgattccaa agcaacaaac acgctagcaa cacagtttgc cttaaattca tttaatcaac
     6661 caatcatttg gttatgtggt ggtttggatc gagggaatga atttgacgaa ctcattcctt
     6721 atatggaaaa tgttcgcgcg atggttgtat tcggacaaac gaaagctaag tttgctaaac
     6781 taggtaatag tcaagggaaa tcggtcattg aagcgaacaa tgtcgaagac gctgttgata
     6841 aagtacaaga tattatagaa ccaaatgatg ttgtattatt gtcacctgct tgtgcgagtt
     6901 gggatcaata tagtactttt gaagagcgtg gagagaaatt tattgaaaga ttccgtgccc
     6961 atttaccatc ttattaaagg gtgtgagtat tgatggatga taaaacgaag aacgatcaac
     7021 aagaatcaaa tgaagataaa gatgaattag aattatttac gaggaataca tctaagaaaa
     7081 gacggcaaag aaaaagatca aaggctacac atttttctaa tcaaaataaa gatgatacat
     7141 ctcaacaagc tgattttgat gaagaaattt acttgataaa taaagacttc aaaaaagaag
     7201 aaagcaatga taaaaataat gattctgctt ctagtcatgc gaatgataat aatatcgatg
     7261 attctacaga ctctaatatt gaaaatgagg attatagata taatcaagaa attgacgacc
     7321 aaaatgaatc gaatgtaatt tcagtcgaca acgaacaacc tcaatcagct cctaaagaac
     7381 aaaatagcga ctcgattgat gaggaaacag taacgaaaaa agaacgaaaa agtaaagtaa
     7441 cacaattaaa gccattaaca cttgaagaaa agcggaagtt aagacgtaag cgacaaaagc
     7501 gaatccaata cagtgttatt acaatattgg tattgttgat tgctg
//
LOCUS       DANRDI010000125         7521 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.125, whole genome shotgun sequence.
ACCESSION   DANRDI010000125 DANRDI010000000
VERSION     DANRDI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.125"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(176..484)
                     /locus_tag="PZ377_002104"
     CDS             complement(176..484)
                     /locus_tag="PZ377_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358966.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(628..894)
                     /locus_tag="PZ377_002105"
     CDS             complement(628..894)
                     /locus_tag="PZ377_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358967.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(1178..2986)
                     /locus_tag="PZ377_002106"
     CDS             complement(1178..2986)
                     /locus_tag="PZ377_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDM6358968.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPVIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            3103..3495
                     /locus_tag="PZ377_002107"
     CDS             3103..3495
                     /locus_tag="PZ377_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HDM6358969.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            3497..6358
                     /locus_tag="PZ377_002108"
     CDS             3497..6358
                     /locus_tag="PZ377_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HDM6358970.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKKDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQK
                     GKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKY
                     EDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTA
                     GYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
     gene            6664..7437
                     /locus_tag="PZ377_002109"
     CDS             6664..7437
                     /locus_tag="PZ377_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDM6358971.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
BASE COUNT         2844 a          951 c         1270 g         2456 t
ORIGIN      
        1 tgattccaat tttgactatt ttagctagtt taatcaattt gttttgtgat attaaaaagc
       61 ataaatgatt ctatattaat gcaactttgt attcgtacaa ggttgtattt ttttcgcaaa
      121 caattactta ctgacagcgc atcaataaat ctaaccaaca cactatcaca aacatttatg
      181 ggtattttgt aattttataa aagcaaacgt agaataatgc gataagtaat aatgctatcg
      241 ttaaaaataa gaatgctaat gcaataaaat catattcaaa gcttagaatt gaagacatta
      301 aaaatactgc actcaaactt atacatagcg taaatatata tgcaatttgg ttcttgtgtt
      361 tatctgcaat ggccgtttga tttataaaag agattagtgt gaaatcttta gttattgcga
      421 cataaatgcc taatataacc atacttatgc agaatacgat actaaatacg atgttgacgg
      481 tcattgttgc accttcctga tagaatataa tgtactgtct tttagtaatg gatgcgtttc
      541 ttatatatgt atgtttctaa aaaacagtac gtttttatca ttttttaatt tattagttga
      601 atgaatgggg ttatgaaaaa ggaagtttta atcttttgtc atgaaataaa tgggatgaat
      661 atcacgacta gaagtaatgt tacgaacagg agcgtataaa tctagagacg ctaaattcga
      721 catagtatgt tgctcaatta tggctgatga tgaatttaaa gtatgtgcgt tggaactgtc
      781 ggatttttgt tcataatgtt ttgcatattg cgccatgatg aatagtgtaa aaataaataa
      841 aacaataaga gatataatgc ccataatcaa aagtatttgt ttagagcctt tcattatttc
      901 acatcctttc taaaatatat ttgtaactaa atttaaaata gttatttttg taattctaaa
      961 cctttttcat cgcgaaaaca attaaatagg tcgcggtatt aattattata ttattaccgc
     1021 ttaatatgaa aaatacatga aaattaattt tctaatatac ttttgaaaaa ttattacaaa
     1081 ttagcccctt caaaacgcga aaacataagg attctagttt caaaagggct gataagcata
     1141 aaatgaaatg taatatttcg atgtataaaa tttttaatta gctaaaaatc atcgcattaa
     1201 ttttttgagc tacatcatca aaattcggac attttaacga cacatataat ttaattttag
     1261 gttcagtacc agaaggacgt aaagcgataa atccttcgtc aaataagaca cgaataacat
     1321 ttgatttagg agaattaatc tgcgacgttg tatctttatc caaatgataa acctcgctag
     1381 ttaaataatc ttcaattgct ttcactttga gtccttgaat ctcttgcggt ggatttgaac
     1441 ggaatttggt cattattgca ttaattttct ttttcccttc aaatccttct agcgtatgcg
     1501 agaataatgt atcctcatgt ctaccaacag tttgataaat ctgttctaat tcatctttca
     1561 atgttttgcc atataatttt aactcagaag cgtattttat aatgactggc acaatttgta
     1621 cggcatcttt atcacgtaca aaaggctctg atagaaaacc gtaactctct tcaaatgcga
     1681 aaatcatatt ttgatgatca tccagttgtc ttatttcctg agcaataaat ttaaagcccg
     1741 tcagcacctc tttgtattca acattattat agcgtgctaa tgactttgtt aactcactac
     1801 ttacaataga ttgaatcatt aagcggtgac gtaattgaga cgtttgttga atacgataat
     1861 taagtaacag cgcaccaatt tggttgccat taaaatatgt gatatgaccg tgtgcatcac
     1921 gttcagcaat accaagtcga tctgcatcag ggtcagttga tattaataag tctgcatgac
     1981 ttttatttgc aagttcgact gcttgatcaa aagcgcgatg atcttcagga ttagcacttt
     2041 gaactgagct gaaattcgga tcaggtttac attgtgcttc tacaagatta aattgattga
     2101 aattcaaaga ttgtaataac tcaggtacaa tcggaacact tgtaccatgc aaactagtga
     2161 acacaacttg taaatcagat ttaggaatat agccaatcat attttgaatg tgtttcatat
     2221 agtcatcagt tacagatttc ggaaaaggct tgatataaga tgtattttgt ttagaaatag
     2281 gtatatcaat ctgtaatgga tcgccaactt cttcgatata acgacttgca agctcagatg
     2341 catcagtcga taattgcgca ccatcagaac catatacttt gatgccgtta tagtctttcg
     2401 gattatgact tgctgtaatc ataatgccag cagtagtatt aagattgcgc accgcgaacg
     2461 ataattccgg tgtcgtttta tatgtatcag gtaaataaac tgttatttga tggtttgcta
     2521 atacattagc aattatttgg gcgaattcag ttgaaagatg tctaatatca taatgaatga
     2581 ctattgttgg actgtttgtt tgggcattta aataacgcgc taaacctaat gccaattttt
     2641 caatagtaaa cttattaagt cgaccttcac caagaccgaa ttttccgcgt atacccgcag
     2701 taccaaatgt taatttactt tcaaaacctt ctcgctgttc aatgtcagat tgctgctcat
     2761 aaaaatgttt aactaaacta tcattagctc gttctatcca taattcttta tccattgttg
     2821 ctaaacatcc tttcaaaatc tcagttagac ttaataaaac atgaaaacta aagcccttac
     2881 atttatgtaa tgaattataa agaaatacgc cccaaaagta aaaaaacaca gccccaagac
     2941 aatacttttc acaagtatta tataatagat gtgtatgaaa atgcatggag tagagcgttc
     3001 atgtgaatgt aaaatattaa tttttaatat tgatttatat atagttttgt aatttttgat
     3061 agaataacaa ttaagatgat tttaaattga agggtgagtc ttatgaaaaa agtaatcaat
     3121 gtagtaggag ctattatttt ttctgataac aaaattcttt gtgcacagag aagtgaaaaa
     3181 atgagtctgc ctttaatgtg ggaatttcct ggcggtaagg ttgaaaagaa tgaaactgaa
     3241 aaagacgctt tgattagaga aattagagaa gaaatgaaat gtgatttaat tgttggagac
     3301 aaagttataa ctacagaaca tgaatatgat tttggaattg ttaggttaac aacatacaaa
     3361 tgtactttaa acaaagagtt accaactcta actgaacata agagtattga atggttgtca
     3421 ataaacgaac ttgataaatt aaattgggcc ccagcggata taccagccgt taataaaatt
     3481 atgactgagg gataatatga gtagattact aaatgatttc aatcaatctt tacataaagg
     3541 gtttatagac aaacatattt ctcataaagg gaattataca ccgaagcttt tagtaaataa
     3601 taaaaatgaa aaagttcttt caacaattat agatgaactt caaaaatgtg aaacgtttta
     3661 tttttctgtg gcttttataa cagaaagcgg tttagccagc ttaaaggctc aactattaga
     3721 tttaagcaat aagggtgtta aagggaaaat attaacatct aattacttag ggtttaatag
     3781 tcctaaaatg tatggagaat tacttaaatt aaaaaatgta gaggtaagat taactgatat
     3841 tgctggattc catgccaaag gatatatttt tgagcataag gattatagtt ctatggtaat
     3901 aggaagctct aatttaacat ctaatgcgtt aaaggttaat tatgagcata atgttttact
     3961 gtctactatg aaaaatggcg atttagttga tagtgtaaaa agtgaatttg acttgttatg
     4021 gcaaaagagt accccactga ctgagcaatg gattaattca tataaagaat catttgagta
     4081 ccgttcctta gaaaaattag cggaggttga acaaactcaa atgctgctag ctgataaggt
     4141 gaaaaaatca gtggaaattg tacccaattt aatgcaagca gaagcattaa ggtcattaaa
     4201 agctattagg gataaagcca aagataaggc gttaataata tctgcaactg gtacaggtaa
     4261 aacgatttta tgtgcattag atgttagaga agtaaaccct aataaatttt tatttattgt
     4321 tcataatgag gggattttaa atagagctaa ggaagaattt aaaaaagtat tacctataaa
     4381 aaatgatagt gattttggat tgttgacagg aaaacataga gatgtcgatg ccaaatattt
     4441 atttgcaacc attcaaacat tatctagaga tgataatttt aagcaatttg atgaaaatga
     4501 atttgattat attgtttttg atgaagcaca tcgttctgct gcatctactt atcaacgagt
     4561 atttaactac ttcaaaccta agttcatgct gggaatgact gctacaccag aaagatcaga
     4621 tgaattaagt atatttgaac tgtttgatta taatattgca tatgaaataa ggttacaagc
     4681 agcattagaa agtgatattt tatgtccatt tcattatttt ggtgtgactg attatgtaca
     4741 tcaaggtatt aaagaagatg atgtaactaa actgagatat ttaacttctg atgaaagagt
     4801 taattatatt attcaaaaga aagattacta tggatattca ggtgaaattt tacaaggatt
     4861 gatttttgta agtagcaaaa aagaagctta tgatttagcg gataaactaa gttcaaaagg
     4921 gattaaaagt gttgctttaa caggagatga tagtgttaat tatcgccaaa ttgtaataga
     4981 aaaattaaaa gaaggtaaga ttaactatat tataactgta gatcttttta acgaaggtat
     5041 tgatattcca gaagtcaatc aagttgtaat gttgagacca acagagtcta gtattatatt
     5101 cattcagcaa cttggtagag gattaagaaa aagttcaaat aaagagtatg ttacagtaat
     5161 tgattttatt ggtaattata agacaaatta tttaattccg attgcgcttt ctggggatca
     5221 atcgcaaaat aaagataatt ataagaagtt tttaacgaat aacgattcga ttaatggagt
     5281 atctacaatt aattttgaag aagttgctaa aaaacagatt tacaattcat tggatgcagt
     5341 atcattaaat caaaataaat taatattaaa agcttatgaa gaagttgaaa atagattggg
     5401 acacatgccg ttactaatgg atttcataca acaacattct atagatccaa gcgttatatt
     5461 ttctaaattt agtaattatt acgagttctt agtgagatat aaaaaaatag atacattatt
     5521 gacggaaaat gaatctaaaa atctggtttt cttttcaagg caaatagcac ctggattgaa
     5581 aagaatcgat agtttagttt tagaagaact tttaaaaaat gaattaacat atgatgaatt
     5641 aaaaaataaa atgttgaacg aagttaagga tataacagaa gatgatatag atacttcatt
     5701 aagaatttta gatttttcat tttacaacgc aggaattgaa aaaatctatg gaagtcccat
     5761 tatagaacgt aatgaaagaa tgataagact aagcgatgca tttactaatg ctctaagtaa
     5821 tcaaacattt aatatgtttt tagaagatct tattgaatta tcaaagtata acaatgaaaa
     5881 ataccaaaaa ggtaaaaatg gactgatttt atataataaa tattcaagag aagattttag
     5941 taaaatattt aattggaata aaaatggttc gtctgtaatc atgggataca tgataaaaag
     6001 ccaagaaatg ccaatattta tcacttatga taagcatgag gatataagtg atagcacaaa
     6061 atatgaagat gaatttttaa gtcaagatga attaaaatgg tttactaaat ctaatagaac
     6121 gctagaatca aaagaagttc agaaaatttt gtcccataga gcaaaaggaa ttaaaatgta
     6181 tatttttgta cagaaaaaag atgatgatgg tatatatttt tattatttag gaactgctgg
     6241 atatatagaa ggttcagaaa agcaagataa aatgccgaat ggttcaaacg tagtaacgat
     6301 ggatttagca ctggataaag ctgtaagaga tgatatatat cgttatctaa caaattaatg
     6361 taaaccgaaa aatgaatgtt agtaaagttg tatttaaaca tcaattttaa taagtaaata
     6421 cattgagttt cagtgttatt attttaaata taagcgcaat ttatcttaat taattagttg
     6481 gttaggccaa taaaaattat aaagatattt gcgcttttta aataatttgt gatgttgata
     6541 tgtgaaaaat acaactaaca attaaaatcc acgaggattt aatcataaaa tttcctctaa
     6601 tattattaat attggaattt gaacttaact ttgcatcaaa aataaaaatg gggatgtgaa
     6661 atgatgattc actcaaaaag gttgaggctc tggctgtatt tagtattatt agctgttttt
     6721 ataggggctt gcggaatgaa aaaggaagaa agcagtaaag ataaacaaat taaagaaaac
     6781 ttcaacaaaa cgttgagcct gtatccaact aaaaatcttg aagactttta tgataaggaa
     6841 ggctttcgag atcaagagtt tgaaaagggc gacaaaggca cttggataat tcactctaaa
     6901 atgacaatag aaacaaatgg aaagaatatg gaatccagag gattggtcct gtatgtagac
     6961 cgcaatacca gaacgacaaa aggtgaattt attgtgaggg aattatggga agataaaaaa
     7021 ggatactcac gtagtaaaga aaaagaatat ccagtaaaaa tggaacataa taaaattatc
     7081 ccaaccaaac cgatagctga tgataaatta agaaaagaaa tcgaaaactt taaattcttt
     7141 gtacaatacg gtgactttaa agatattaat gattataaag atggtgacat ttcatataat
     7201 ccgaatgtgc caagttattc agcaaagtat caattaagta atgatgacta caatgtgaag
     7261 caacttagaa agaggtatga tataccaact aagaaagcac ctaaattatt aataaaaggc
     7321 gatggtgatt taaaaggatc atctatagga cacaaaaatt tagagtttac atttatagaa
     7381 aataaagaag aaaacatcta ttttacggat agtattaatt tcaaacctac tgaatagggt
     7441 taatagaagt ttgaaatgaa aatttcataa agtgttcaaa ataatttttc gctttagttg
     7501 tgtaaattaa agattgagtc a
//
LOCUS       DANRDI010000126         7492 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.126, whole genome shotgun sequence.
ACCESSION   DANRDI010000126 DANRDI010000000
VERSION     DANRDI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.126"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..245
                     /locus_tag="PZ377_002110"
     CDS             <1..245
                     /locus_tag="PZ377_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDM6358972.1"
                     /translation="DFTLMPMGLNILLVLLYFVYLGLTIVMIVFLCFDSKPANKYGES
                     PKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            476..1375
                     /locus_tag="PZ377_002111"
     CDS             476..1375
                     /locus_tag="PZ377_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6358973.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            1368..2600
                     /locus_tag="PZ377_002112"
     CDS             1368..2600
                     /locus_tag="PZ377_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6358974.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            2584..2769
                     /locus_tag="PZ377_002113"
     CDS             2584..2769
                     /locus_tag="PZ377_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358975.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            2818..3144
                     /locus_tag="PZ377_002114"
     CDS             2818..3144
                     /locus_tag="PZ377_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDM6358976.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            3174..4193
                     /locus_tag="PZ377_002115"
     CDS             3174..4193
                     /locus_tag="PZ377_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDM6358977.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(4429..5082)
                     /locus_tag="PZ377_002116"
     CDS             complement(4429..5082)
                     /locus_tag="PZ377_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDM6358978.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            5256..5942
                     /locus_tag="PZ377_002117"
     CDS             5256..5942
                     /locus_tag="PZ377_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDM6358979.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(6008..7267)
                     /gene="sdpC"
                     /locus_tag="PZ377_002118"
     CDS             complement(6008..7267)
                     /gene="sdpC"
                     /locus_tag="PZ377_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDM6358980.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
BASE COUNT         2606 a         1016 c         1385 g         2485 t
ORIGIN      
        1 gtgattttac attaatgccg atgggtctta atattttgtt agtacttctt tattttgttt
       61 atttaggcct aacaattgtt atgatagtat tcctatgctt tgacagtaag ccggcaaata
      121 aatatggcga aagcccaaaa tacccgtcta caattaaaaa tcaaccagtg acatctgaaa
      181 caccggaaaa agcgtgtaca tctgaaacac cgaaaacacc ggaagaaaca gataatcaat
      241 tgtaatgata taaatatctc ggcacaatga tgctgggata tttttttaca aataaggcaa
      301 taaaggcatg gaaaaaacat atctagtgct aatgatactt aacatgcaaa aatggcacta
      361 tttaaatttt tcaatttcag aaattttaaa aataaattca ttttaaaaat attgcattta
      421 tagagaatgg aaatgttata ttaagataaa tacatatttt tttaggaggc aaagtatggg
      481 acttgtaatt gaacatgtta cgaaacgttt tggcaagatg acggcagtaa atgatatttc
      541 attaaagtta gaatcaggta aaatgttagg ttttctagga agaaatggtg caggtaaaac
      601 aacgacattt cgtatgattt taggcttaag tgaaccaact gaaggacaca ttacgtataa
      661 cggtaaaaaa ctagataaaa caatgtacaa tcgtattggt tatttaccgg aagaacgcgg
      721 tttacatggg aaattgacag ttgaagaaga attgaaatat ttggcaactt taaagggaat
      781 gtcaaaaaca gaaatacaac agcaaatatc gtattggctt gagcgttttg atattactga
      841 aaaccgcaaa aaacgaattg atagtctatc aaaagggaat caacaaaaaa ttcagctgtt
      901 agcaagtatg ttacataaac cagaattact aatattagat gagcctttca gtggtctaga
      961 cccagttaat gttgagttat taaaagaagc agttaaagac ttaaatgatt ggggcagtac
     1021 aatcgtttat agttctcacc gtatggagca tgttgaagaa ttatgtgatg atgtttgtat
     1081 tttagataaa ggtcaacttg ttgtttctgg tgatatcaat catgtacgtg catctaatgg
     1141 taacaagaaa gttgtcattg aatcagaaac aacactacca gatttaacaa acattagagg
     1201 catcattcat agtgaaaaca tgaagcaagg tcttcagttg acgattgaga atgaagacgt
     1261 agccaaagat atttatcaag ttgtggcaca tcaaggttat gttaaacgat tccaagttgt
     1321 agaaccatca ttacaagata tattcattga gaaggttggt ggcaaggatg cataagtttt
     1381 gggcgacatt tactttgaca tatatcaaaa aaattaaagc taaatcattt gtaattttta
     1441 tgattattat tgctgcctta atgattggcc tatctaacat tgataagatt attaacatgt
     1501 ttgatgatgg tccagataaa attggtgtag cagcaccgaa tgaacaaatc tataaggttt
     1561 tcaagcaaca ggcgaataca tttcatagtg atgctaagtt tactaaagtt tctatagaag
     1621 atgctgaaaa agaagtaaaa aaacataaac ttgataaagc ttatattatc aaggtgaatc
     1681 aaaatcgcac gttacaaggg acaatcatta gtgagaagcg agtaagtcat gaagattcac
     1741 aaaaagtaca agcactgctt acagctattc aaacaaatat ggtagccggg gaattaaata
     1801 tcaacaaaga agacttacaa aaattacaag cacaaagtaa agttgataat aaggtgattt
     1861 ctaatgatga agtcgataaa gtatcagaag gacaaaagat tttcaactat gctttagctt
     1921 atggcattat ctttctaatg ttcttcattg ttttaaatta tgcatctcaa atagctatgg
     1981 aaattgctag tgagaaaaca tcgcgtgtca ttgaaatgat tattacaagt atttcaccaa
     2041 tccaacatat ttttgctaaa attttaggcg ttatagcagt tgccgtgact cagattattt
     2101 taattgtatt gatggcagtt atatgcattt ttgcctttga cttaaaagac ttgctacaag
     2161 gttttaacgt ggaaatgaat caattgtctt ggcaaattat tattgtcgga attataagta
     2221 gtattgttgg aattacggcg tatgtattac ttgcagccat cctcggatca ttaacatcta
     2281 gaatagaaga tttaaatcaa tctttgatgc cactgacatt acttggtatg attgcatttt
     2341 atatcgcgat attcacgata aataatcctg atggacaact tgctttaatc acaagttata
     2401 ttccgttttt agcgccattt caattagttg tacgcgctca aacttcaggg cttcaaatat
     2461 atgaggtcat cttaagttct ttaatctcaa ttgtaatggt agctgtcttg atatggattg
     2521 caattaaaac atataaagat tcagtactta catttgaacg tggcttattt aattcaatga
     2581 aacgtgtatt taaaaaataa aattgatgag agtgacacac atttatcata taaaatgatg
     2641 atgaaagcgt gttactctta tttttttata tttagctata gaataatgtc taaaaaagtg
     2701 tacaatatga atgtgaaaac gaacttttta aaatgggaat gtgaagtaag aagaaatgta
     2761 aacaaatgat atgtagccga tagattattg atagcaaatg tgtgatggag tgatttcatg
     2821 ttatatccga tttttatatt tatattagct ggcttatgtg aaataggtgg gggatacctg
     2881 atttggctgt ggcttaggga aggacagtct tcacttgttg ggctaatagg cggtgcgata
     2941 ctcatgttat atggtgtcat tgcgacattt caatcatttc catcattcgg aagagtatat
     3001 gcagcatatg gtggcgtatt tatcatcatg agtttgattt ttgcgatggt cgtagataaa
     3061 caaatgccag ataaatatga tgttatcggt gcaatcattt gtattgtagg tgttttagta
     3121 atgttgctac ccagcagagc ttgaacactt gtggttagaa gggagaagta aaaatgatag
     3181 ttgaagttga gcaccaaaga caaggtgtag atttaatcaa aattgataat gatgaaacta
     3241 aaattatttt tacgaattat ggagcaagag tagtgtcttg gaaatatcat gataataata
     3301 ttgttcttgg aaatgttgtt gaagcggatg aattttatca tagtaatcct tttaaatttg
     3361 gtgcttctat aggaagatat agcggtagaa ttgataatgc taaatttaag ttgaaaggta
     3421 aagaatatca gttagaaaaa aacaacggtg aacatcattt acatggtgga tgtcatggtt
     3481 tggacaataa gttatttgat tatgaaatta gaaatgaaat tgctcaaatt aaagttatat
     3541 ttaagacggt gttgaaatca gctgatgatc atttcccggg tgatatcgat gtgacgatta
     3601 cacatattta tgatgcggat caccaatggt cgattgaata cgaagcagtt gcatcagaag
     3661 acacattgtt tagtcctacg aatcatgtgt acttcaattt gaatcgagat aataatgtcg
     3721 ttgataatca tcgcatttca agtaatcaat tagatatgta tgtattagat gagcgaaata
     3781 ttgttacagg tgatattcta gatttacatg aagtgtttga agataacaag ataaaattga
     3841 gtgatatttt tacatcacaa catgcgcaat taagccaaca aatgacacgt tttggtggtc
     3901 tcgatcaccc atttacagtt ggtgaacata aaatgtatgt tgaaaatcat gaatttatgt
     3961 tagaggtcga tacagatatg cctcatgttg tgttctttac ttttaaccag cctgatgagt
     4021 gggatagtcc atttaatatt tataaaccac attctggctt tacattagaa acacaatttt
     4081 taccaaatga tattaattta tatggtgaaa aagcaccgtc aattttaaaa gctggtgaag
     4141 tgttcaattc gaaaacaagt ttccgtatcg aagaaaaagt agaaaatgat taaaagtatc
     4201 ggcatttata taatatttat gttatttggc ggaattttga ataggttatc aagaatcgta
     4261 tcaaatgaag tgacatcatt aatatacagt ttgaaataaa gaaaatatat aacataaaaa
     4321 aggaatcaat agcatcagtg gttatagata agactattat acaaacgatg tgtaagttga
     4381 ttcctttttg ttttgaaaag ttcatttaac acttataatg aaaggtaatt atttcaatgt
     4441 tctgttatac attttttgaa gtttatcgcg tcttgttggt gacagaaatg gattgtttag
     4501 tgcatatcgt aacatatctt gattttgtct atcattataa taaaggtcat taagctgttg
     4561 tacagataaa cgatgttcga ttggtgcttc ttgtattaag taaagctgat catttggaga
     4621 tacatagcct tgttttaata cccggaaata gaaacctgtt ttaccagatg tagacatgcg
     4681 cttcactaaa tcaggaatat tatattttgc ttgaattttc cagcatggtt ctctaatttc
     4741 agaaacctca attaacgctt cacctaaacg atattgatta ccaaagtaaa catcagattc
     4801 gtctaaataa tcaaatgtta aattctctcc aaacatcgca tgagtaggta gtgtaggtaa
     4861 atcatcttga tacagtgcat aattatgctt actaaaccca caaactgctt tatgtggtcc
     4921 accatgatct ttataagcct gttcatcacc agtgaaacca gttgaagata accatgttgt
     4981 ttgtgaaatc ttagttttat ctaaagcaga gcgcatcggt cttttcgagc tataaggcaa
     5041 atcttggatt tttcctgtcg aaattgcgtg aattgatatc atatcattct ccttaacgag
     5101 tattatttaa aatatattgt actataaaac atttttagaa ttgtaaattc tgattgatat
     5161 gtttaaaaaa atgttagaaa taagggtatg ttaatttata cgagtcatgt aaaatatgaa
     5221 ttgaatagat tacataaata tgggaggcta ccttcatgaa agatgtcaaa gcactcaagt
     5281 taatgacatt aaatgatgtt ttaagtcaaa tcaatggtga catgacgctg ggaattggta
     5341 caggaagtac aatggaatta ctcttacctc aaatggcgca actaattaaa gaacgtggtt
     5401 acaatattac aggtgtatgc acttctaata aaattgcatt tttagcaaaa gaattaggta
     5461 ttaaaatatg tgaaatcaat gatgttgatc atatcgactt agcaattgat ggtgctgatg
     5521 aagtagatcc atctttaaat ataattaaag gtggcggtgg tgcgctgttc agagagaaag
     5581 ttatagatga aatggcatcg cgttttgttg tggttgtcga tgaaacgaaa atagttcaat
     5641 acttaggtga gacgtttaag ttaccagtag aggtggataa atttaactgg tatcacatat
     5701 tacgtaaaat tgaatcatac gctgatataa aagtagaacg tcgtgtaaac gaagatgtcg
     5761 catttataac tgataatggc aattatattt tagattgtaa gttaccaaaa ggaattgacc
     5821 catataagtt ccatgagtat ttaattcatc tgacaggtgt gtttgagaca ggatacttct
     5881 tagatatggc agatcaagtt attgtaggca ctcaagaggg tgttaaaatt ttagaaaaat
     5941 aatatatagt atagaaaagc gcttgctagc atcatctttt taagaggtac tagcaagcgc
     6001 tttgttatta tttgttttta tctgaagatt gttcttcagt tttttcgata tgtttttctg
     6061 catcaattac aactgatgaa cgtctgtctt ctttataagt cgcaggttct tttaatgatt
     6121 gctctatttc atcttcattt gaatcgtttg ttaaatcgtc actttgtggt gcttgtttaa
     6181 ctgattctgc tgattctact gactccgaag tgatgtcatg atcttcatca tgattagatt
     6241 cagtttgatt acctatgtga tgttgaggtt ctgttttttc agtaccttca tgaatgcttg
     6301 gttcgtcagc cactgtattt tcagtatcat ttttagcaac accaaccgtt gatgcagtag
     6361 ctacacctgt tttttcagca tctttaatat ttgaagaaga tttttcagcc tcagtatgat
     6421 tagtttcttc atcgtcattg acatggtcta aataattatt aggctcaagt tcttcaaggt
     6481 ttcgtcttgt tgttaaatat gcaatacctc ggataattaa gcttaaacca atgtatccta
     6541 ctgcaacgat tgctgttgaa atggcgatga ctttgattga aaatagatcg ccgatttctt
     6601 cgctaaacaa gaaaataagt ccgaatgtca ttgaagcatg gaatgtcgtt gcaatataaa
     6661 ttgtacgtcc tttagtcgct ctaattaatt caccaagaat catagagaat gagaatgtat
     6721 aaaggaagtt ataagcagca aattctgtac cataagttgt atttgctgag aaaactgaat
     6781 acatcaaacc aacaacaata cttgcaaaga acgtgttcat tttcgtttca acaatatttt
     6841 gtaagtatga acggaatccg aattctacta cgaacgccat cagaatatgt ccaatcagaa
     6901 tgtgtgtaat aggtactgat aagcctgttg attgtaataa aataaagcta tctgcaaatg
     6961 tattaaagct gtacatacca ataattagaa taattaaagg taaaattaaa gctagcaata
     7021 gacgttcaat tactttcagg ctgattgaga attttaaacc tgcaagttgg acctttttat
     7081 atttgaaaac gagtatacaa atgattgcag caattaatgg tgctagatct gtaacttcaa
     7141 atataaagtg tttgacacca attatagact ggaaatctct gagcataatg gataacgcca
     7201 ttgtaatgac aaagaagacg aaaatcgtca ttgcccattg aaaaccagaa attttattgt
     7261 tcttcattat atgtaacctc cattaggtaa caagcaaaat atcttttagt aacaattata
     7321 catattacaa gccgagagat aaattgtcta tgtcaatatt aatgaaaatg taatagtctt
     7381 gacgtatttt attaacataa atcagctaag tattcgttat tacatagaaa ataggggtaa
     7441 aagtaatgta gataagtatt tttaagatac ggatttaact atgaattgaa tt
//
LOCUS       DANRDI010000127         7273 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.127, whole genome shotgun sequence.
ACCESSION   DANRDI010000127 DANRDI010000000
VERSION     DANRDI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.127"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(172..888)
                     /locus_tag="PZ377_002119"
     CDS             complement(172..888)
                     /locus_tag="PZ377_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDM6358981.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(996..1721)
                     /locus_tag="PZ377_002120"
     CDS             complement(996..1721)
                     /locus_tag="PZ377_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HDM6358982.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            complement(1816..2541)
                     /locus_tag="PZ377_002121"
     CDS             complement(1816..2541)
                     /locus_tag="PZ377_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HDM6358983.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            2979..3980
                     /gene="argF"
                     /locus_tag="PZ377_002122"
     CDS             2979..3980
                     /gene="argF"
                     /locus_tag="PZ377_002122"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDM6358984.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            4003..4935
                     /gene="arcC"
                     /locus_tag="PZ377_002123"
     CDS             4003..4935
                     /gene="arcC"
                     /locus_tag="PZ377_002123"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDM6358985.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            5107..6663
                     /locus_tag="PZ377_002124"
     CDS             5107..6663
                     /locus_tag="PZ377_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDM6358986.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            6971..7198
                     /locus_tag="PZ377_002125"
     CDS             6971..7198
                     /locus_tag="PZ377_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358987.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
BASE COUNT         2423 a         1007 c         1266 g         2577 t
ORIGIN      
        1 tgacaacgaa ataaatttta taaaatacta tttctgtcac attcctactc ccaattccat
       61 ttaaatatat agaaaatttt acctattata tacatcatac actttaaaaa ttatcattta
      121 aataatctga tttggaatta aagtgcatga agtataagtc accttcatat actaatcaaa
      181 gaggacgtca acagttattt tattaggatt tttaacataa acatttgcta gatctgaatg
      241 taatcttttg cttaaatcaa tagtgtagtt attaccgcca ccggtgatct taagcttacc
      301 tttattacga ttttcgttat ataatatttt attttttatt aacgcttctc gtgcacggaa
      361 atcgatttct ttcaatgtta atactggttt attgccttgg tatattttat gcgcaccaat
      421 aatcgtttgt agtttatctt tgtattgcac aaaaagatta taagttttat cagaaggttt
      481 tgcggctggt gtaacgccac ctgtaaatgt ctctctataa gaccaccata actgatcagt
      541 atctttgtct tttagtccaa acacatctac gtaacgatct tttaactgat taatatttcc
      601 ccaactttca gcgccccata aagatatatg tgctgaccat gaatatctct taagttcaac
      661 ataaatgttt ccattatcat attgatatag ccatttattt gaaaatgaaa aatgaggctg
      721 tgtgtaatat ttaattaatt cattgatgtt agtttcatct tgaccaatgc tataagcttt
      781 agcttcagag taaaaactaa atactggttt ttgattttga ggtaatacag tacctaatag
      841 taataatgtt gtcgttaaaa ttatattttt cgtgatgttc ttactcatta gaacatctcc
      901 tttcagagga atcatgatac gaggaataag aaatttaaat gtgagcgaag tcaatatagt
      961 atttgcgatt atttttatta actccattat tattgttagt ttgatttttc gaggataact
     1021 tcaatttttg cattttgagg ttttttaaca tatctatttg catcagttga tggcaacctt
     1081 ttacttaaat ctattgtgta gttattgtct gcacctgtta ttttaatttg tcctttatta
     1141 taagaattat tatataattt tttactttta attaatgttt gacgaatacg aaaatctaat
     1201 tcttttaaag ttaaaacagg cttattgcct tcataaactg gaaatccgcc agtaaacgtt
     1261 tctgctttat ctttatatgt tacattcagt ttatagtgtt tatcgttaga tgttgctgca
     1321 ggagtaacac caccagtaaa cgtttcttga gataatgcaa aagaatcaat ggtttcttgg
     1381 tcttttatgc caaaaatatc aacgctttta tttcttaatt ggttgatatt gccccaactt
     1441 tcaggtccat aaacttgaat atgactatac caagaaaact gtaacaacgt tgcatgaatc
     1501 gtaccgttat ctttttgcca taacgtactg ttagagaagg ttaaatattt ttgcgagtaa
     1561 tatttagtta actcattaac gttagtttcg ttttgattta tataataagc tttcgcttca
     1621 gatgaagaat tgataggtgt attaggaaat tgtgtagatg ctgtacctaa tagtaacaat
     1681 gttgttgata aaataatttt tttcgtgatg ttattgttca ttcgaatttc tcctttgagt
     1741 attgttggaa tgtttaatta taaaaagggg ttaattagat aattgaaatt atccgcattt
     1801 acaaaaggta ataggttagt tagatttttc gagtatgact tcaatttctg cattacgagg
     1861 atttttaaca taacggtttg tgtcagttaa ttttaacttt ttacttaaat caatcgtgta
     1921 attatttcca tcagcagtta tcttaatttg acctttatta aattctccgt tatataactt
     1981 tttatttttt attaatgttt gacgaatacg gaaatctaat tcttttaaag ttaatactgg
     2041 tttatttcct ttgtaaaatt catgtccacc gatgatagtt tgttgtttat cactatattt
     2101 taaaaatagt ctataaggct tatcagatga agtagctgct ggcgtaacac caccagtaaa
     2161 tgtttcatca taagtccagt aaccttcaac tgtgtcctca tctttagttc caaatatatc
     2221 aacgtattta tttcttaact gattaatgtt tccccaactc tcggatccaa acacttgaat
     2281 atgactatac caaacccacg tttgcaatgt tgcatgaatg ctaccattgg gcttttgcca
     2341 taaccatttt ccagataatg aaaaatgcgg ctgagtgtaa tatttgatta gttcattgat
     2401 attagtctcg ttttcactga tattataggc ttttgcttca gatgaaaaac tgattggtgt
     2461 tttaggaagt tgtgttgatg tagtttctaa aagtaacaat gctgttgata aaactaattt
     2521 attcatgatg ttctttttca tatgaaaatc tcctttgcgt gaattaccca aagtatataa
     2581 gctattacac cgattcggaa ttaaataaaa gctaaaacta tgttaaataa acttaaacag
     2641 ttagtagtgt tatttaagca aaacttatca tttttaagtt ttggacagaa acagtatcta
     2701 ataaagtagg cgggagttat atattaaaaa cgatacgtta gaattattct ctaatcaatt
     2761 gtattaaatt gtttgataat tgaattttct aactacttga aaaatagtta tactttaaat
     2821 gtagtactta ttttaattat ttcctactac ttaaatttaa tattaataaa atgttcattt
     2881 aattattgat aaaatattac aaattttaat agtaggttgt gtttattttg tatgcgctta
     2941 caatttaggt gtaactaaaa taaaaggagt tgttattaat gaaaaattta cgaaacagaa
     3001 gttttttaac tttattagac ttttcacgac aagaggtaga attcttatta acactctccg
     3061 aggatttaaa acgtgctaaa tatattggca ctgaaaagcc tatgttaaaa aataaaaata
     3121 ttgcactgtt atttgaaaaa gattctacaa gaacgcgatg tgcatttgaa gttgcagcgc
     3181 atgatcaagg tgcaaatgta acttatttag gcccaactgg atcacaaatg ggtaaaaaag
     3241 aaacaactaa agatactgca cgtgtgcttg gtggaatgta tgatggcatt gaataccgtg
     3301 gtttttcaca aagaacagta gaaactttag ctgagtattc aggcgtacca gtgtggaatg
     3361 gtttaactga tgaagatcat cctactcaag ttcttgctga tttcttaaca gcaaaagaag
     3421 tcttaaaaaa agattatgca gatattaact ttacatatgt tggagatggt cgtaataacg
     3481 ttgcaaatgc attaatgcaa ggtgctgcca ttatgggtat gaacttccat ttagtttgtc
     3541 caaaagaatt aaatccaaca gatgaattat taaatcgctg taaaaatatt gccgctgaaa
     3601 atggtggcaa catattaatc acagatgata ttgaccaagg tgtaaaaggt tcggatgtaa
     3661 tttacactga tgtttgggta tcaatgggtg aacctgatga agtatggaaa gaacgacttg
     3721 aattattgaa accatatcaa gtaaataaag aaataatgga taaaactggt aatccaaatg
     3781 ttatttttga gcattgctta ccatctttcc ataatgctga tacgaaaata ggtcaacaaa
     3841 tttttgaaaa atatggtatt cgagaaatgg aagttacaga tgaagtattc gaaagtaaag
     3901 cttcagttgt attccaagaa gctgagaaca gaatgcatac aatcaaagca gtcatggttg
     3961 ctacattggg tgaattttaa atgatataag gaagtgaata tgatggcgaa aatagtagta
     4021 gcattaggtg gtaatgcttt aggaaaatca cctcaagaac aactcgagct tgttaaaaat
     4081 actgcgaaat cattagtagg attaataaca aaaggacatg agattgttat tagtcatggt
     4141 aatggaccac aggttggaag cattaatttg ggacttaact atgctgcaga acataaccaa
     4201 ggtccggcat ttccatttgc tgaatgtggc gcaatgagtc aagcttacat cggctatcaa
     4261 ttacaagaaa gcttacaaaa tgaattgcat tctattggaa tggataaaca agtggtaaca
     4321 ctagtgacac aagttgaagt tgatgaaaat gatccggcat ttaacaatcc ttcaaaacca
     4381 attgggttat tttacaacaa agaagaagct gaacaaattc aaaaagaaaa aggatttata
     4441 tttgttgaag atgctggaag aggatataga cgcgttgttc cttcaccaca acccatctct
     4501 attattgaat tagagagtat taaaacactt attaaaaatg atacactcgt tattgctgct
     4561 ggtggtggag gtataccagt aattagagag caacatgatg gttttaaagg tattgatgca
     4621 gttatagaca aagataaaac aagtgcgttg ttgggtgcta atattcaatg cgatcaattg
     4681 attattttaa cagcaattga ttatgtatat attaatttta acactgaaaa ccaacagcct
     4741 ttgaaaacaa caaatgttga tgaattaaaa cgatatatag acgaaaatca atttgcaaaa
     4801 ggaagtatgt taccaaaaat tgaagcagcc atatcattta ttgaaaacaa tccaaaagga
     4861 agtgtgctta taacatcatt aaatgaatta gatgctgcct tagagggtaa agtaggtact
     4921 gtgattaaaa agtaattgaa ttgaaacgct tttcaattac tatatgtcaa atgcatgatt
     4981 tttattattt atgtgcaccc cctgaaaata atgcctctat tttgatgcgg ggtgcacttt
     5041 cttaatttat atttataaaa tctttaaggt agaaatgcta ggttaaatgt cgaaggagat
     5101 gaaaccgtgg aaaatacaat taatgaaagt gaaaagaaaa aacgatttaa attaaaaatg
     5161 ccaggtgcat ttatgatttt attcatttta acggttgttg cagttatagc aacatgggtt
     5221 attcctgctg gtgcatattc taaactttct tacgaacctt catcccaaga actaaagata
     5281 gttaaccctc ataaccaagt gaaaaaggtt ccgggtacgc aacaggaact agacaaaatg
     5341 ggggttaaaa ttaagattga acaatttaaa tcaggtgcaa ttaataagcc ggtatcaatt
     5401 ccgaatactt atgaaagatt aaagcaacat ccagctggac cagaacaaat aacaagtagc
     5461 atggttgaag gtacgataga agcggtcgat atcatggtat tcattcttgt actaggggga
     5521 cttattggcg tagttcaagc cagtggttct tttgaatcgg gattgttagc tttaacgaag
     5581 aaaacaaaag ggcatgaatt tatgctaatt gtgtttgtat caatactaat gattatcggc
     5641 gggacattat gtggtattga agaagaagct gtagcatttt atccgatttt agtccctata
     5701 tttatagcgt taggatacga ttctatcgtt tcagttggtg ccatattcct tgccagctct
     5761 gtcggtagta cattttcaac tattaacccg ttctcggttg taattgcctc taatgccgct
     5821 ggtacaactt ttacggatgg cttgtattgg agaataggtg cttgtattgt cggtgcgatt
     5881 tttgttatta gttatttata ttggtactgt aaaaaaatta aaaacgatcc taaagcgtca
     5941 tattcttatg aagacaaaga tgcttttgaa cagcaatggt ctgtattaaa agatgacgat
     6001 agtgcccatt ttactttgcg taagaagata atccttacat tatttgtact accatttcca
     6061 attatggtat ggggagttat gacgcaaggt tggtggttcc cagttatggc ttcagcattt
     6121 ttaatattta caattataat aatgtttatt gctgggacag gtaaatctgg attgggagaa
     6181 aaaggaactg tagatgcatt tgtcaatggt gcatcaagtt tagtaggtgt atctttaatt
     6241 attggtttag ctcgaggtat taatttagtg ttgaatgaag gtatgatttc agatacaatc
     6301 ttacactttt catcatcttt agttcaacat atgagtggac cattatttat catcgtatta
     6361 ctatttattt tcttctgttt aggttttatc gtgccatcat cttctggctt agcggtatta
     6421 tcaatgccta tctttgcacc actagctgat acagtaggta taccaagatt cgtcatcgtt
     6481 acgacatatc aatttggtca atatgcgatg ttattcttag cgccgactgg acttgttatg
     6541 gccacactac aaatgttaaa catgcgatat tcacattggt tccgatttgt atggccggta
     6601 gttgcttttg tattgatttt cggtggcgga gtactaatta cgcaagtact aatttattca
     6661 taatttgaaa tgctatatta taaaaaatac taatggggtt ttatgcatct cgtaggtttg
     6721 tagaaatact aaactaagcg aggtgcatta ttatttttga ttaagaaaat aatgacggta
     6781 atgataacac tagtaagtaa ttgatacaat gctctattta ataatgatat ttttaaaatt
     6841 tgtttttaat gtaatgttag atctatggta tattatattt atcgtggtaa atatgtattt
     6901 gctgtaaaca gtttttgtaa cacatgatac atatggttgt gaaaatagca tatataaagg
     6961 atggctataa atgacacatt tgacaaaggt tttagataca ctaactggaa tatgcgtagt
     7021 attattattt agtaaatatt ttgtggcgta tgcaaatatg gtgtttgatt ggaatttaag
     7081 atggtatttg ctagaaaaca taccacattt gccaattata ttatttattc tgatgtttat
     7141 tttcggagta ccttctgaaa tgataaaaga taggcaaagg aaaaataacg gtgtttaatt
     7201 tatcgatatt tagaggtgat aaaatttgtc aactattaca aagactaaaa taaaaatctt
     7261 tattaattat ttt
//
LOCUS       DANRDI010000128         7087 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.128, whole genome shotgun sequence.
ACCESSION   DANRDI010000128 DANRDI010000000
VERSION     DANRDI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.128"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            47..682
                     /locus_tag="PZ377_002126"
     CDS             47..682
                     /locus_tag="PZ377_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDM6358988.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            1061..1948
                     /locus_tag="PZ377_002127"
     CDS             1061..1948
                     /locus_tag="PZ377_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDM6358989.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            2096..3046
                     /locus_tag="PZ377_002128"
     CDS             2096..3046
                     /locus_tag="PZ377_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDM6358990.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            3101..3820
                     /locus_tag="PZ377_002129"
     CDS             3101..3820
                     /locus_tag="PZ377_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDM6358991.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            3804..4604
                     /locus_tag="PZ377_002130"
     CDS             3804..4604
                     /locus_tag="PZ377_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6358992.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            4740..5246
                     /locus_tag="PZ377_002131"
     CDS             4740..5246
                     /locus_tag="PZ377_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358993.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            5408..6124
                     /locus_tag="PZ377_002132"
     CDS             5408..6124
                     /locus_tag="PZ377_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6358994.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            6293..7081
                     /locus_tag="PZ377_002133"
     CDS             6293..7081
                     /locus_tag="PZ377_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDM6358995.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
BASE COUNT         2628 a          897 c         1256 g         2306 t
ORIGIN      
        1 cgataatatc atttctgtcc cactccctct aaaatggagg gtgtaaatgt taggaactga
       61 tgaattatat aaagttttat atgaacatct cggaccacaa ttttggtggc ctgctgataa
      121 tgacattgaa atgatgttag gtgcaatttt agttcaaaat actagatggc gaaatgcaga
      181 aattgcattg aatcagatta aagaacatac gcattttaat ccaaatcata tattagaact
      241 acctattgaa acgttacaat cattgataca ttcaagtggc ttttataaaa gtaaatcact
      301 gacgattaaa acattattaa catggttagc acgacatcat ttcaattatc aagagattaa
      361 tgagcgatat aaaggtggat taagaaaaga attattatct ttgaaaggta ttggaagtga
      421 aacagcagat gtcttacttg tttatatatt cggacgtatt gaatttattc cagatagcta
      481 tacaagaaaa atatatgata aattaggata tgaaaacact aaaaattatg atcaattaaa
      541 aaaagtagtc acattaccaa atcattttac aaatcaagat gctaatgaat ttcatgctct
      601 gttagatgta tttggtaaac attactttag agacaaagat ataaagaatt atgatttttt
      661 agaaccttac tttaaaaagt aaacgctgtg aagttagata gatgagttta tatgaaatat
      721 aaaaaataat ttactatttt cttttagtat gtggacttat ataataaata gaagcatata
      781 aagaaaaaaa cagttgtttg tttgtgcagc aactgcataa gagcccctaa tcgctaaagc
      841 tcaaggggag taaaggaata cagttgtttg tgcagcaact gcataaaagc ctctaatcac
      901 taaaggtgaa gaggaacgca gttggatgct aaggcacaac tgcataaaag cctctaatcg
      961 ctaaagatga agaggaacgc agttggatgc taccgcacaa ctgcataaat ccctctaatc
     1021 gctaaagcga aaagtgggat taaaaaggag atgtgatagt gtgaagaaat cgttaattgc
     1081 ttttattttg atttttatgc ttgtcctgag tggctgtggt atgaaagata atgataaaca
     1141 aggtagcaat gataatggct cgtctaaatc gccgtaccat agaattgttt cgttaatgcc
     1201 tagtaatact gaaattttat atgaattagg attaggtaaa tacatagttg gtgtttcaac
     1261 ggttgatgat tatccaaaag atgtgaaaaa gggtaagaaa caatttgatg ctttgaatct
     1321 aaataaagag gaacttttaa aggcaaagcc agatctaatt cttgcgcatg agtcgcaaaa
     1381 ggcaactgct aataaagtat tgtcatcatt agagaaacaa ggcatcaaag tagtgtatgt
     1441 taaagatgca caatcaattg atgaaactta caacacattt aagcaaattg ggaaattaac
     1501 gcatcatgat aagcaggctg aacaacttgt tgaggaaact aaagataata tcgataaagt
     1561 catagattca attcctgctc atcataaaaa atcaaaagta tttattgagg tttcatcaaa
     1621 gcctgaaata tatacagcag ggaagcatac attttttaat gatatgttag aaaaattaga
     1681 agcccaaaat gtgtatagtg acattaatgg ttggaaccct gtaacgaagg aaagtattat
     1741 taaaaagaac ccagatatat taatttcgac ggaagctaag acaagatcag attatatgga
     1801 tatcatcaaa aaaagaggtg gattcaataa aattaatgct gtcaagaata cacgtattga
     1861 agttgtaaat ggtgatgaag tatcaagacc aggtccacgt attgatgaag gattaaaaga
     1921 attaagagat gcaatttata gaaaataaac cattctaatt atgcccctta ttgctacatg
     1981 taaaaaatac atgtttgaga taaggggttt ttaaaatata tttagtgaat gatagcaacg
     2041 cgagtatgtg attgctataa tgaatgtaat tatcgatgaa agaaaagaga atgctatgac
     2101 atttaataaa gtattattga gctggatagt catattgatt ataacaacta gcatatatct
     2161 attttggcag ttgggcgata tcaatgatgt atttaaccag tctattttaa tcaatgttag
     2221 attaccgaga ttattagaag cattgttgac aggtatgata ttaactgttg caggccttat
     2281 atttcaaaca gttttaaata atgcattggc agatagcttt acattaggat tggcaagcgg
     2341 cgctacattt ggttcaggat tagcattatt tttaggttta acaacgttat ggattcctgt
     2401 attttcaata acatttagtt tgataacatt aataactgta ttagtcatta cgtcggtatt
     2461 gagccaaggc tatccagtta gaatcttaat attaagtggt ttaatgattg gtgcgttatt
     2521 caattcactt ctatattttt tgattttatt aaaacctcgc aaattaaata caattgccaa
     2581 ttatctgttt ggtggttttg gtgatgcaga atactcaaat gtatctataa tagcaatcac
     2641 atttatcatt gcattgtttg gtatatttat cattcttaat caactaaagt tattgcaatt
     2701 aggagaacta aaaagtcagt cactaggctt aaatgttcaa ttgattacat atatcgcgtt
     2761 atgtatagct tctatgataa cggcgataaa tgtcgcatat gttggcatca ttggattcat
     2821 tggtatggtg ataccgcaac tcattagaaa atggcagtgg aaacaatcat taggaagaca
     2881 attggcttta aatattgtaa ctggaggaca aataatggtt atggcagatt ttattggtag
     2941 ccatatattg tcaccagtac aaataccggc aagtattatc attgcattaa ttggtatacc
     3001 agtgttattt tacatgctaa tatctcagtc gaaacggtta cactagcaca cgacatttgc
     3061 taaaataaaa ataactataa acataaagag ggcataagcg atggatttga atcaaattaa
     3121 agcagttgta tttgatttag aaggtacgtt gttggacaga gttaaatctc gagagaaatt
     3181 tatcgaagag caatatgaac gatttcatga ctacttaatt catgttcaac tggcagattt
     3241 taaaaaagca tttattgagc tagatgacga tgaagataat gataaacctg atttatataa
     3301 agaaatcatt aaacgtttcc atgtagatag gttaacttgg aaagacttat ttaatgattt
     3361 tgaaatgcat ttttatcgtt atgtatttcc ttattacgat actttgtata cactagaaaa
     3421 gctatcgcaa aaaggctttc aaattggtgt tatcgcaaat ggtaaatcta agattaaaca
     3481 atttcgatta cattcacttg gtttgatgca tgttattaat tatttatcaa catcagaaac
     3541 agttggtttt cgtaaaccac atcctaaaat ttttgaagat atgattgatc aactaggggt
     3601 attacctgag caaattatgt atgttggcga tgatgcgtta aatgatgtag ctccagcacg
     3661 agctatgggc atggttagtg tatggtataa acaagaagat gctgaaattg aaccactcga
     3721 agaagaagtt gattttacaa ttacaacagt ggaagaatta ttaaccattt taccaataaa
     3781 aaatgataat aaaggagaaa attatggatc tatttactag aaaagatgga acatcgatac
     3841 attacagtac attaggtgaa ggctatccta tcgtattgat tcatactgta cttgataatt
     3901 attctgtgtt taataaatta gcagcagaat tagcaaaatc atttcaagtt gtgttaattg
     3961 atttacgtgg acatggctat tctgataaac ctcgtcacat tgaaataaaa gatttttctg
     4021 atgacattgt tgaattactt aaatatttat atattgaaga agttgcattt gtatgccatg
     4081 aaatgggtgg aatcattggt gcggatattt cagtacgtta tcctgaattt acatcatcac
     4141 ttacgttggt aaatccaaca tctattgaag gtgaattacc ggaagaacgt ttatttagaa
     4201 aatatgccca tattattcga aactgggatc ctgaaaaaca agataaattt ttaaataagc
     4261 gtaagtatta tcgtccgaga aaaatgaatc gattcctcaa acatgtcgta gatacaaatg
     4321 aaatatcaac taaagaagaa attcaagcag ttaaagaggt attcaaaaac gctgatattt
     4381 ctcaaactta tagaaatgtc gtagtaccga caaaaattat tgcaggagaa ttcggtgaaa
     4441 gaacaacaag attagaagct aaagaagtag ctgatttaat ccaaaatgcg gactttgaag
     4501 tatatcaaga atcaagtgca ttcccatttg ttgaagagca agaaagattc gtcgaagata
     4561 cagctgcatt tatcaacaaa catcacgatg aaaagcatgt ttaattttta gtgataagtg
     4621 aaatataaaa gtagtaatta ttatttgtaa tataattgta atatgactgt tgttttagaa
     4681 atgattgttg tataatggtt gtgagcacaa gacgatataa ataaaaggag agagtgctta
     4741 tgaaaaagct actaacggca agtataattg catgttctgt tgtaatggga gtaggcttag
     4801 tgaacactag tgccgaagca gcaagtggca actctattga tactgttaaa caattaatta
     4861 agggtgatca gtcattagaa aatgtgaaaa ttggcgaatc tattaaagat gttttaacta
     4921 agtacaaaaa tccgatgtat tcttacaatg aagatggaac tgaacattat tacgaattcc
     4981 atactaaaaa aggtatgtta ttagtaacta ctgatggtaa gaaaaacaat ggtaaagtaa
     5041 ctcatatttc aatgatgtat aatgatgcta atggtccaac atatcaagct gttaaaaatt
     5101 atgttggcaa agcagtaaca catacggaat atagcaaagt tgctggtaat tttggatata
     5161 ttgaaaaagg caaaacgact tatcaatttg cctcagcacc aaaagataaa aacataaaat
     5221 tatatcgtat tgatttggaa aaataataat taaattaatc gtgcttaacc gacaagttaa
     5281 atcgcaatac ttgtcggtta ttttattaca tggcataata cagtgaatat taatttttat
     5341 gatttgcgaa ggggaaatta aaccaataca ataaataaag atgtttcatt ttaaaggagt
     5401 actaccaatg cgtattaacg ataaaatttt actagaaaat atagaagatt actttaatca
     5461 taaaggttta tcaccgcatt tgattgatga tattaaagag aaagtaatta ctgatataaa
     5521 aaattctgaa aagaaagatc aagattatat tgaatataaa agaaaatctc cagcacaaat
     5581 catattaatg attcaaagga atttgtttgc tttacaaatg aatccagtta ttttctttat
     5641 tataaacttc attctcatat cctatttata tgataaacag tatgttcagt ttcaagctat
     5701 tactggaatg agtctatttt attgtttagt gatttttcca atgactattg ttgtatacct
     5761 aagggtgtct caaaagaatt acttgcgtag taataaaata gaaatgatta tgggtacaat
     5821 tatcgctatc atatccttgt tattaattat attacaagca tttaatatta cttggggcgt
     5881 tataccaatt acaaattttg gacatcaatt tttctttttc attggtatta ttttagtaat
     5941 tgccggcata ttttataagc gacttgagtt ttcgggaatc gggttattat tttgtcaaaa
     6001 aaccgtcgat gcaatgattc ataatccaca atcagcccag attttttcat taattatatg
     6061 gatattatta gtagttctag ttatatattt cacaattaga ttatcttcac gtacaagatt
     6121 ataaatatga taaaactatt cacttgatta attgtattaa ttgagatgaa tagttttttt
     6181 attgttggaa taacttttgg taatttataa ataatttaaa aaaattgttt ataaaatgga
     6241 agcgtatata gaatgaaggt tgggtatata gtttattgag ggaggtgtca caatgaataa
     6301 agtcacaatt aatcctcaaa tccaattaac ttatcaaatt gaaggtaaag gggatcctat
     6361 aatattactt catggattgg atggtaattt agctggattt gaagatttgc aacatcaact
     6421 agcatcatca tataaagtac ttacttacga tttaagaggt catggcaagt cttctaaaag
     6481 tgaatcatac gatttaaacg atcacgttga ggatttaaaa attctaatgg agaagttaaa
     6541 tattcatgag gcacatattc taggacatga tttaggtggg gtagttgcta agttatttac
     6601 agataaatat gcttatcgtg taaaatcatt aactaccatt gcatcgaaga aagatgactt
     6661 aatacacagc tttactcaat tgttaataca atatcaagat gatatagcgg gttttaataa
     6721 gtctgaagcg tatattcttt tattttctaa attgtttaga aatcaagaga agacgatgaa
     6781 atggtatcaa aaacaaagaa tatatagcat taagtctgag gatgatagtg cggtggcaat
     6841 tcgttcatta attttgcata aagatgaacc tatgtattta aaaaaacgta catgtgtacc
     6901 tactttgtta attaatgggg aacatgatcc tttgattaaa gataagaatc attttaaatt
     6961 ggaagcgcat tttttaaatg ttacgaaaaa aatcttcgaa cattcaggac atgcaccgca
     7021 tattgaagaa ccagaagcat ttatgaatta ttatttaaaa tttttaaaaa gcgtatcata
     7081 atatgtg
//
LOCUS       DANRDI010000129         7015 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.129, whole genome shotgun sequence.
ACCESSION   DANRDI010000129 DANRDI010000000
VERSION     DANRDI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.129"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..243
                     /gene="rpsN"
                     /locus_tag="PZ377_002134"
     CDS             <1..243
                     /gene="rpsN"
                     /locus_tag="PZ377_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDM6358996.1"
                     /translation="ERKREELVNKYYELRKELKAKGDYEALRKLPRDSSPTRLTRRCK
                     VTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            400..1377
                     /gene="guaC"
                     /locus_tag="PZ377_002135"
     CDS             400..1377
                     /gene="guaC"
                     /locus_tag="PZ377_002135"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDM6358997.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            1399..2415
                     /locus_tag="PZ377_002136"
     CDS             1399..2415
                     /locus_tag="PZ377_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDM6358998.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(2795..3418)
                     /gene="lexA"
                     /locus_tag="PZ377_002137"
     CDS             complement(2795..3418)
                     /gene="lexA"
                     /locus_tag="PZ377_002137"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDM6358999.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            3561..3794
                     /locus_tag="PZ377_002138"
     CDS             3561..3794
                     /locus_tag="PZ377_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359000.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            3930..4169
                     /locus_tag="PZ377_002139"
     CDS             3930..4169
                     /locus_tag="PZ377_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDM6359001.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            4290..6278
                     /gene="tkt"
                     /locus_tag="PZ377_002140"
     CDS             4290..6278
                     /gene="tkt"
                     /locus_tag="PZ377_002140"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDM6359002.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            6556..6798
                     /locus_tag="PZ377_002141"
     CDS             6556..6798
                     /locus_tag="PZ377_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDM6359003.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            6785..6883
                     /locus_tag="PZ377_002142"
     CDS             6785..6883
                     /locus_tag="PZ377_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359004.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
BASE COUNT         2584 a          959 c         1264 g         2208 t
ORIGIN      
        1 gagagaaaaa gagaagagtt agtaaataaa tattacgaat tacgtaaaga gttaaaagca
       61 aaaggtgatt acgaagcgtt aagaaaatta ccaagagatt catcacctac acgtttaact
      121 agaagatgta aagtaactgg aagacctaga ggtgtattac gtaaatttga aatgtctcgt
      181 attgcgttta gagaacatgc gcacaaagga caaattccag gtgttaaaaa atcaagttgg
      241 taaaatcaaa tttcgtactt tagcccattt acaatatcaa taaaacaatg tacagtatat
      301 acgaatgcta taaactgaat gttttctcat attaataagg aaacattcgg tttttaattt
      361 gcatttaaaa ataattagtt tttaaagggg ctatttaaag tgaaaatatt tgattacgaa
      421 gatattcaat taatacctaa taaatgcata gttgaaagta ggtctgaatg tgatacaact
      481 atccaatttg gtccgaaaaa attcaagcta cctgtagttc ctgcaaatat gcaaacagtt
      541 atgaatgaga aattagcgaa atggtttgct gaaaatgatt acttttatat catgcatcgt
      601 tttgatgaag aagcaagaat accttttata aaacatatgc aaaattcagg cttatttgca
      661 tctatttcag ttggtgtaaa gaaagcggaa tttgatttta ttgaaaagtt agctcaagaa
      721 aaattaatcc ccgaatatat tacaatagat attgcgcatg gtcactcaga ttcagtgata
      781 aacatgatta aacatataaa aacccatata cctgatagtt ttgttattgc tggtaatgtt
      841 ggtacgccag aaggtgttag agaattagaa aatgctggtg ctgatgctac caaagtcggt
      901 ataggtcctg gtagagtttg tattacaaag attaaaacag gttttggtac tggtggttgg
      961 cagttagcgg cattaaacat atgtagtaaa gcagctcgta aacctttgat tgccgatggt
     1021 ggtataagaa cgcatggcga cattgctaaa tcaattagat ttggtgcatc aatggtcatg
     1081 attggttcat tatttgcggc acacgaagaa tcacctggtg aaactgtaga acttgatggt
     1141 aaacagtata aagaatattt tggtagtgca tctgaatttc aaaaaggcga acataaaaat
     1201 gtagaaggta aaaaaatgtt tgtagaacat aagggttcat taatggatac cttaaaagaa
     1261 atgcaacaag atttacaaag ctcaatttca tatgccggtg gaaaagactt gaaatcatta
     1321 cgtactgtag attatgttat tgttagaaac tctattttca acggtgatag agattaatat
     1381 ttatagtagg tgatgtaaat taaaaaattc atagtaactg ttgttgcgtt tttatcaatt
     1441 attatcattg cgccaataac agaatttaaa ccattcattc atttacaaaa tgaagtaaga
     1501 caatatattg acattcacat caataaagaa acaatttctg cggaaaataa attggataca
     1561 ccgaagaaac aacaatttgc ctttaataat atacaaatga acatgtcgaa atcagatgtt
     1621 gagaaaacat taaataaacc aaaaagagtg acatttaatg aatatggtac gaagtggtat
     1681 acgtattatg atgacgatta caataatttt ataatgataa gttacatgaa agataaagtt
     1741 aatgcgttat atacaaatca aaatataatc acttcaaaat caaaaattaa atataataca
     1801 cctaaatcgg ttgtaaggca aagattaggc gaaccagaaa cagagattgt taaaggtaga
     1861 gtgcgttacg aacaaaataa taaagaatat gatgttttcc ataaaaatca catttatacg
     1921 acggtatttt atgataagca tcgacgtaat aatgtaacag ctgttttaca agtaagtgat
     1981 gctatggaaa atagattaaa agaacaatat ggagcaccat cgaaatcgct tgcagatagt
     2041 tttgaactac aaaattttga tttagttaat gctgaaagaa aacaacatca attatctaca
     2101 ttgaagtatt ctaaacagaa ttctgaaact gcacgtaagc atagtaaaga tatggccaac
     2161 aatcattatt ttgatcatac aaatttaaaa ggtcaatcac catttgatcg attgaaaaaa
     2221 gatggtatta catttaactc agccggagag aatttagcat atggtcaagt tagtagtatc
     2281 tatgcacatc aaggattaat gaattctatt ggtcacagaa aaaatatttt aaatgatacg
     2341 tttaaaatat taggtgttgg tgttgatttt aatgatgaaa aacaaccttt ttggacagaa
     2401 aattatactg gttaatgttt ttggcatgag attaaagggt aatgtttgtc tgtaaggaag
     2461 tagataaaca tgatgacaac agataaacca aaagatgcgg atatacttga acgcgtcaaa
     2521 gatatactta ataaaaaaga aagaaaaaaa taataatggc tcgctcctgt aaattattac
     2581 gggggcgagt tttaatttgt gtaacatttt gcagaaatct gtaattcaaa atttatatta
     2641 aagaatgaga ttatctaaca attcgcaatt tctgtttgaa ctgaatttag attagaataa
     2701 ataaaacctc ttgaaaacgt aactttttca agaggtccaa aatttataaa aatgagggct
     2761 taacaattac gataatatat aacggtttaa aatattacat ttcgcggtac aaaccaatta
     2821 ctttcccaat tacagcaaca ttgtctaggt aaattggctc cattgtacta ttttcaggtt
     2881 gtaatcgata acgatttttt tctttataga agcgtttgac agttgcttca tcttcctcag
     2941 tcatagcaac aataatgtct ccattttctg ctatggtttg actgcgaaca attactttgt
     3001 ctccgtctaa tataccagcc tcaatcatac tgtcgcctac gacgtttaat atgaatatgt
     3061 cgctattgtg tgtcgatgtt aagtgttcag gtaatggaaa atattcttca atattttcta
     3121 ctgcggtaat aggaacacct gctgtgactt taccaataac tggcacatga atcgtttctt
     3181 ccatattaat attatcattt gtttgatcac ttacaatttc tatagcacgt ggtttcgttg
     3241 gatctcttct tatatagcct ttttcttcaa gacgtgaaag gtgaccatga acagttgaac
     3301 tggatgctaa gccaactgct tcaccaattt cgcgaacact aggcggataa cctttcgttt
     3361 gaacaacttg tttaatatag ttatatattt cgctttgtcg ttttgttaat tctctcatat
     3421 ataggcactc cctaaattaa tttaaataca gtatatcatt aatttgaaac caaaacaaac
     3481 atttgttcgt taaaatattg acacagaaca taagttctga taaattacta atacaaacaa
     3541 atgttctagg agtgaaaatg atgttttaca ataaatataa aaacgtatca acatatatca
     3601 tcatattttt agtttcaagt gcagcctttg caatattctt gttaagtgcg aacattagtg
     3661 ctcactcgga acaagtgtac gaaatgactg accatcaaat taagaacaat acgataaata
     3721 aagcatacga acataaagac cctacaaaca atagcgaaca aagagatggg aaagtgttcg
     3781 ctttaataaa ttgatacatt gtcacaacgt tattttgcct atttttgcga aatagcgttt
     3841 tttattactt ttttgctgat cttaaatttg ttatattttg ttaaagtatt ataatgattg
     3901 aataaacaaa ttgaaggtag gttttttaat tgagtaattc tgatttgaat atcgaaagaa
     3961 ttaacgagtt agctaaaaag aaaaaagaag taggattaac tcaagaagaa gcaaaggagc
     4021 aaacagcctt aagaaaagct tatcttgaga gttttagaaa agggtttaaa caacaaattg
     4081 aaaatactaa agtaattgat ccagaaggta atgatgtaac acctgaaaaa attaaagaga
     4141 tacaacaaaa aagagataat aaaaattaaa tcacaaatct gtaaagaatt ttctgacatt
     4201 ataacttgaa ataagtattt tacttatctt tttattttaa aataagttat aatgtatttg
     4261 ataaaattga agaagggaag atacacaaga tgtttaatga aaaagatcaa ttagctgttg
     4321 atacgctacg tgcactaagt atcgacacaa tcgaaaaagc gaattctggt catccaggat
     4381 tacctatggg agctgcccca atggcttaca ctttgtggac acgtcatctg aattttaatc
     4441 cacaatctaa agattacttc aatagagacc gtttcgtatt atctgcaggg catggttcag
     4501 cattattgta tagcttgtta catgtttctg gtagtttaga attagaagaa ttaaagcaat
     4561 ttagacaatg gggttctaaa acaccaggtc atcctgaata cagacataca gatggtgtag
     4621 aagttactac cggaccactt ggacaaggtt ttgctatgtc agtaggatta gctttagcag
     4681 aagatcacct agcagggaaa tttaataaag aaggatataa tgttgtagat cattacacat
     4741 atgtattagc ttctgacggt gatttaatgg aaggtatatc gcatgaagca gcttcatttg
     4801 ctggacataa taaattaagt aaattagttg ttttatacga ttcaaatgat atttcattag
     4861 atggcgaatt aaacaaagct ttttctgaaa acacaaaagc tcgttttgaa gcatatggtt
     4921 ggaattactt actagttaaa gatggtaatg atttagaaga aattgataaa gcgattacta
     4981 cagctaaatc tcaagaagga ccaacgatta ttgaagttaa aacaacaatc ggatttggtt
     5041 caccgaataa agcaggaact aatggtgttc atggggcacc tttaggtgaa gttgaaagaa
     5101 aattaacatt cgaaaattac ggtttagatc ctgaaaaacg ttttaatgtt tcagaagagg
     5161 tatacgaaat tttccaaaat actatgttaa aacgtgctaa tgaagatgaa tctcaatgga
     5221 attcattatt agaaaaatat gcagaaacat atcctgaatt agcagaagaa tttaaattag
     5281 cgattagtgg taaattgcct aaaaattata aggatgaatt accacgtttt gaactgggtc
     5341 ataatggtgc atctcgtgct gattctggta ctgttattca agcaatcagt aaaactgtcc
     5401 cttcattctt tggtggatca gcagaccttg ctggttcaaa caaatccaat gtaaatgatg
     5461 caactgatta tagttctgaa acacctgaag gtaaaaatgt gtggtttggt gtacgtgaat
     5521 ttgctatggg tgctgctgta aatggtatgg ctgcacatgg aggtttacat ccatatggtg
     5581 caacattctt cgtatttagt gattatttaa aaccagcgtt acgtttatca tcaattatgg
     5641 gattaaatgc aacgttcatc ttcacacatg attcaattgc agtaggtgaa gatggtccta
     5701 ctcatgaacc aattgagcaa ttagctggat taagagccat tccaaatatg aatgttatcc
     5761 gtcctgctga tggtaatgaa acaagagtag catgggaagt tgccttagaa tctgaatcta
     5821 cacctacttc attagtattg acacgtcaaa acttaccggt attagatgta ccagaagatg
     5881 tagttgaaga aggcgttcga aaaggtgcct atacagttta tggctctgaa gagacaccag
     5941 aattcctatt attagcttca ggttcagaag ttagtcttgc agttgaagct gctaaagatc
     6001 ttgaaaaaca aggtaaatca gtacgtgttg tttcaatgcc taactggaat gcatttgaac
     6061 aacaatctga agaatataaa gaatcagtta ttccatcaag cgtaacaaaa cgtgttgcga
     6121 ttgaaatggc ttcaccgctt ggatggcata aatatgtagg tactgcaggt aaagttattg
     6181 ctattgacgg ctttggcgca agtgcacctg gcgatttagt agttgaaaaa tatggattta
     6241 caaaagaaaa tatcttaaac caagttatga gcttataaga ataatttata aagcgagtat
     6301 gtttagaagt ctaggatgca taatcttagg cttcttttaa agtgttgaaa tttagagtat
     6361 agcacttaaa ctacatcata agtgataagt tatgaaagta tactatttca gattaatctt
     6421 taaaagctct gttataacag catgattttt gatattattt ttagtatcga tattaaaata
     6481 cttgaataaa ctagttcttg aaataatgtg atgaatttag taaaattcag taggataaag
     6541 aaagcgggtg aaacaatggc aacttggtta gcaattattt ttatagtagc tgcattaatt
     6601 ttaggtttaa ttggaggttt ccttttagct agaaaatata tgatggacta cttgaagaaa
     6661 aacccaccaa tcaacgaaga aatgcttcgt atgatgatga tgcaaatggg tcaaaaacct
     6721 tctcagaaga aaattaatca aatgatgacg atgatgaata aaaatatgga tcaaaatatg
     6781 aagagtgcga aaaagtaaat tcgcaattga tagaggctat tttccagata tggaaatggc
     6841 ctctttttat aatcaaatta ataagaataa atatgtttat taaaattaaa gttaacaaaa
     6901 tgacgaatag actgagaaat gctataattc attttgtatg atttacagag agtttattta
     6961 acgagaaggt gtctgcgtgc tctatttaat attttcaatc attgtagctt tattt
//
LOCUS       DANRDI010000130         6979 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.130, whole genome shotgun sequence.
ACCESSION   DANRDI010000130 DANRDI010000000
VERSION     DANRDI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.130"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(150..1004)
                     /locus_tag="PZ377_002143"
     CDS             complement(150..1004)
                     /locus_tag="PZ377_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDM6359005.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(1280..1504)
                     /locus_tag="PZ377_002144"
     CDS             complement(1280..1504)
                     /locus_tag="PZ377_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359006.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            1703..2173
                     /gene="sigS"
                     /locus_tag="PZ377_002145"
     CDS             1703..2173
                     /gene="sigS"
                     /locus_tag="PZ377_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HDM6359007.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            2286..2729
                     /locus_tag="PZ377_002146"
     CDS             2286..2729
                     /locus_tag="PZ377_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDM6359008.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(2716..3159)
                     /locus_tag="PZ377_002147"
     CDS             complement(2716..3159)
                     /locus_tag="PZ377_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359009.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
     gene            3455..4090
                     /gene="sdpA"
                     /locus_tag="PZ377_002148"
     CDS             3455..4090
                     /gene="sdpA"
                     /locus_tag="PZ377_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HDM6359010.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(4530..5243)
                     /locus_tag="PZ377_002149"
     CDS             complement(4530..5243)
                     /locus_tag="PZ377_002149"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDM6359011.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            5503..5805
                     /locus_tag="PZ377_002150"
     CDS             5503..5805
                     /locus_tag="PZ377_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359012.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            6061..6426
                     /gene="crcB"
                     /locus_tag="PZ377_002151"
     CDS             6061..6426
                     /gene="crcB"
                     /locus_tag="PZ377_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDM6359013.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            6423..6776
                     /locus_tag="PZ377_002152"
     CDS             6423..6776
                     /locus_tag="PZ377_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDM6359014.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT         2303 a         1143 c          877 g         2656 t
ORIGIN      
        1 ttctttatcg atgtcctgcc ccacttgcat tggtgggatt atcaatcaat gtgttttctc
       61 taatgcaaaa agacgctttc tatcaaaaca agatagaaag cgtcttttta gtatgtccga
      121 gtcataaaaa caactcgtag cttatcaaat tacttattca aatgtttact gtcatcttta
      181 tacacaaagt atttgaagta ttttccttca gtcttcatgt tcttataaaa gtcagcgata
      241 attgttgcat tactttctgc ccacttaata tctgtagcat attgatgttc tcctggattt
      301 tttggattcc atctcatact atacaatgta ttttgatctg tgcttgataa gaagtgctta
      361 tgaatgaaat cagcaccgcc tgaaatagct ttttcaggtg tatcccaacc atgcttttta
      421 gcatattctg cacctgtttt aattgggtct ttatcaaggg ctcctactcc atagaaattg
      481 tagtactttt tgccatcaat ttcgactcca ttagctaatt cacttttaac tgcgccagtt
      541 tctaataatg catgtgaaat taaataaact tcgttaacgt gcttatcttt agcagctttt
      601 aagaaatcat ccgtatgttt caataacgtt ggtctatcta ctaacatacg tttaattcta
      661 tttttatcaa tcccttgata ctttgataaa tctaaaaatt gatatttttg cttttcatta
      721 tcgataaaag taccgctatc cattgcactt ttaatttcag ttgcagatgc atctctccat
      781 gcatcattct ttttatttga tacctgttga ctcgtataat tatttatttg tttctttgct
      841 gcatcgttta atgtaacatt taacttttca atcttaatgt cggatttaac atgtttgaaa
      901 aatatctgat cggatatcat tgagaaaaat aaaaatgaga caacagcaaa tatgacaaca
      961 agtcctatta ttccaaaaat agaacctttc ttgtgtttat tcatatccac acctcttagg
     1021 tcattgttgt tatacattca gtttgactac gataataaag tttaacatta ttaaaaccgt
     1081 ttaacaattt tatctatgca ttgttacata ttcataaata aacagtaatg taaatgaaac
     1141 gttttaaacc atttatcaac attactttac gttgatctac gaatcataaa tgttttatct
     1201 ttttgtataa cctttgaaaa cctttatatc aataatctta ttattcacgt attaaaaaac
     1261 aaaaaattct tttgttcgtt tacttagttt cttttttata atccatcata ataaaacttg
     1321 ctgcatttga tctattttta taatagtact gatttaaatc aatcgtgcct tcaatttgac
     1381 catcacgata catcatttca ttagtgaagt tatttatcat cacgaaatca gcgcgatctc
     1441 ttataacatc taaatcgtcg tttcgtgcaa aaaaatgttc taaatttaaa tgtgcgtaat
     1501 ccattgttac ctcccgatta caaattttgt tctatctact atttagcaaa gtaataatac
     1561 atcatatcac attaaattaa ttacttatgt cttatattta acacttaatt tagcgattac
     1621 gcaaatgaat gatttttcta aatattttat tctaacttta tccggaaata tttgttatac
     1681 gtataacttg gagaatgact acttgaaatt taatgacgta tacaacaaac accacaaaat
     1741 catacaccat cttttaaaaa aatataatat tagctataat tatgatgagt attatcaact
     1801 actcttgata aaaatgtggc aattgagtca gatatataaa ccctcaagca agcaatcttt
     1861 atcctctttt ttattcactc gattaaattt ttaccttatc gatttattca gacaacaaaa
     1921 tcaattaaaa gatgtcattt tatgtgagaa taattcacca acattaactg aacaaccaac
     1981 atactttaat gaacatgacc ttcgtttaca agatatcttc aagcttttaa atcaaagaga
     2041 aagactatgg ctcaaactat accttgaagg atacaagcaa tttgaaattg ctgaaatcat
     2101 gtcattatcg ctttcaacga ttaaattaat taagatgtcc gttaagcgta aatgccaaca
     2161 taattttaat tagtttattg tgaaatttcg gtcgatgtgt taatgatgtt gaaacaccct
     2221 aacatcttca tataaattta ttttaaccat taaattaaca acttattaaa cgaggagtac
     2281 tatatttgca agacaattct actaaatatc tactttatat ccaaactgct acttcaaacc
     2341 atctcgaaac aaattgtgtc tttttacatt gcgattacat tcttaaagtt ccaattaaca
     2401 aactcgtttc atattatgcg aaattgcatt tatcatcaca aagtgtgcta attgagactg
     2461 caaaaaacat actaaatatt aataaactgg ttcctattta catcaaccca aaaactatac
     2521 tttttccgtt aaaacataaa cgtgcaccga tacaaattta tatcaatgca cattatattg
     2581 ttggtatgac tgccatagag aattcaacat taatacattt tcaagaaggc attcagctag
     2641 aagttgatga gccattttct ctagtttcaa aaaaatgtca tgaaagttta gctttgaagc
     2701 acttcattga aaatactatt tcgaattaaa tttaatttct aaaagtttct tagtaaagta
     2761 tgatgtcaca atttttataa aatatatgct agcaaacatt aataaaatcg ctccaataat
     2821 tgttaaaata acaacagttg tagataaatc taccttagga ctaacgaaaa attgtacgcc
     2881 agatagactt gtaattagcc caaataaaaa caaacctaat aaagcaacat aagcaaacat
     2941 catcactact aatgtaacaa acatactata actttttgtt tctttatttc gaaatacatt
     3001 ttgtcttaag taagcattag caaaggtaac aggcttattt aagttaacgc tgtcatcatc
     3061 agatatagaa gacaatcgtt gatctaatgc ttgtttctcc ttcttgttca aaaaccataa
     3121 ttgcttttta acttttttat caaatgatgc tttattcata attaaggatt tctctccttt
     3181 ataatattga cgtaatttta aacattagcg tcaattgaaa gtgtatttta tatattttac
     3241 agaaatatat gtatcaaatc tacaaaaatc catattacaa tttcatttat ggtgatttga
     3301 cgctaaaata cagtctgcta atttttcaaa ctaatgcttt ttaggtaggt acaatgataa
     3361 attttaatcg taaatatgtt tcatatatta tgaatctagc cttaatcata ttgcattcaa
     3421 aataatttta aaaaatgaaa agaaaggatt tagcatgcaa aaattcaaag acttttttta
     3481 cgatgattta tcggttacac gaggaaatta ttttttaact ttaatggcag cattttttat
     3541 tactatcatt ttatttatcg gcatagttgt cagtgaagta catttacttt atagcatgct
     3601 aattgtatta gtaggtttaa ttctattgag gctattcaaa atcaatttat tctcttttaa
     3661 aaaattaaca ttgtctcaag ttatttatat tataggcggt gcactattaa tttatgggtt
     3721 agataatctt tatttatatt ttcatgacgt accggcaaat gaacaacaat tagagcaagc
     3781 aatacgaaat acaccattct atatttctat tttcactgtt accatcatcc ccgctattgt
     3841 ggaagaaatt gtttttcgcg gtatgataat aagggttatc ttcagaaaac acttgttttt
     3901 agggttaatt gtgtctagtt tagtttttgc atcattacac gaatctgaca cttggattgg
     3961 ttatttacct tacttatatt ctggtttgat ttttggccta atttatataa aaacaaaacg
     4021 attagaagtt gtaatattca tgcacttctt aaataacttg ttagctctgc tctttataat
     4081 atggagataa ttttgagata acctttcata ttattttcaa attatctatc tgatatttat
     4141 tttattgaac ttgagtcacc acttaaactc aaaaatacgt gtcatcaagt gcaaaataat
     4201 ttagaactaa tacaaacata aaaactttta ctttacgctt aaaatcaaaa gtcacatacc
     4261 tagatataag tacaacttgt atattttgta ctctaatagg tatgtggctt aaataatatt
     4321 taattcttga gttgcatata gtaactgttt ttattcattt tctaaaatat gcaagactaa
     4381 tttccttttc tactcctgtt aaatcaattt agactttttt ataactcgag cttttactta
     4441 gctgaatttt gacttcttct tcattaataa ctatgatgga aaaaccccca taagcacaat
     4501 gcctatgagg gtttaaatat atttatatat tatagaatag aaagacctga agattgaata
     4561 tctttcgcaa agcctttaac tgtatctact gataattcgt taatatcgcg acctaagttt
     4621 gtattcactt ttttcacaac atctgctggg catgtaataa tatctgcacc aatttcatca
     4681 gcttgaatca cattgaataa ttcgcggcaa cttgcccata ataatttaac gccgtcttta
     4741 ctatgcgtaa cttttacagc ctctttcatt aatggtaatg gatctacgcc tgtatctgca
     4801 atacgtcctg caaatactga aacatatgtt ggcacacctt cagttactgc ttcagttatt
     4861 tctttaactt gttcaattgt gtaaacagcc gtaacgttta atctcacatt gtcagctgaa
     4921 agttttttaa ttaaaggaat cgttgattca ccttttgtat ttacaatagg aattttaaca
     4981 aatacatttt cgccatattg ttttaaaatt gctgcttctt tttccatagt ttctaaatcg
     5041 tctgcaaata cttcaaatga aattgaagca tctggaattt ctttcacagc ttcttcagca
     5101 aaagctttgt aatctgttac gcccgcttta gccattaaac taggatttgt tgtaaaacca
     5161 tccacttgtt tgtttttata agctgctttc atttcttcaa tatctgcacc gtccgcaaat
     5221 acttctatat ttagtttagc catataatat agcctccttg attcttatta aaattttaac
     5281 aacatctgca tgtctttttc ttacaaccat ttgtaaaaaa tgatttttat ttctttgtta
     5341 tataaaacat ttatatcacg cttttataat atatagctaa ttttttgcct atatttttta
     5401 aaaatgactt tagcaaacat tgtgtctaca agttaataaa actattgttt tatgattgtt
     5461 tatgatacga tatgtaaatg ataatcatcc aggaggtcta gcatgtctcg ttcaaaaaaa
     5521 tacttttact tatctagctt aatgattatt ttaagctttt tctttaatac aaataacgtt
     5581 ttcctaagtg gactttttaa ttcttttatt aaattaatac ttttctgcag tgttattaac
     5641 tcaattgtac taattttgtc tataattttt gcagatcgtt caattaaatc actaaagcct
     5701 gatgcagatt ggattagaat tgcgagtaaa agtttgcctt ggattattct aattgttatt
     5761 ttagtacata tcttttcaat tgttcgtaca ttcggtttta tttaaaaaag ttaatatgtc
     5821 attgtagcct taatacaaaa caatacaatg tatcatgcta taatgagtaa aacaatttga
     5881 taacgttgtt gcgtataaaa aatattagat tttcgaaatc ataactatgc atctaatcgc
     5941 tatagttata caacaagata taacatataa tgaggtttga taatgcatcg acaatttttg
     6001 tcgtcgcgtt gccaaaacct cttttttaaa ttcaaactac ttcttttcga ggtgaaccaa
     6061 atgcaatatg tatatatttt tatcggtggt gctttaggcg ctttattacg ttacctcatt
     6121 tcttttctga atactgacgg aggttttcca atcggaacac tgatagccaa tttgactggt
     6181 gcctttgtaa tgggattgct aacagcctta acaattgcat ttttttcaaa ccatccgacc
     6241 ctaaaaaaag ctattacgac tggttttctt ggtgctttaa cgactttttc aacatttcaa
     6301 ttagaattaa tacatatgtt tgatcatcaa caatttataa ctttactact atatgctgta
     6361 acaagttatg tctttggtat tttgttatgt tacgtcggta taaaactagg tggtgattta
     6421 tcatgatatc aatcatttta gtcatgattg gcggcggttt tggcgcaatt gctagaagtg
     6481 ccattactga ttattttaat cataaattta cttcaaagtt acctatcgca acattgatag
     6541 taaatctagt tggtagtttt ttaattggat taactatagg cttatcaatt tcaatctcat
     6601 ggttccctgc gttctttgtt accggttttt taggtggctt aacaactttc tcaacgttag
     6661 ccaaagaact tacactaatg atgacgccaa aatttaatat taaccttttt ctcaattatt
     6721 cacttttaca attcatcatt ggatttatag cttgttatat tggctatcat atttaaaaat
     6781 aaaatgcttc attcagcaaa taggtaaatt acgacacctt cctgaacgaa gcatttttta
     6841 attttcatgc aaatttttaa gcaccatata atgcctacca aatttcaata atctttgttg
     6901 ccgtttaaat aatgtgaatg tcaataaatt ctccaaacta gtcgaaaata aagggagtgg
     6961 gacataaatc cctaaaaaa
//
LOCUS       DANRDI010000131         6967 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.131, whole genome shotgun sequence.
ACCESSION   DANRDI010000131 DANRDI010000000
VERSION     DANRDI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.131"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..201
                     /locus_tag="PZ377_002153"
     CDS             <1..201
                     /locus_tag="PZ377_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 family protein"
                     /protein_id="HDM6359015.1"
                     /translation="SLRAQEKIYDKLDRNFEELKRDKVEDEKNKEKNAKNIRDIKMWI
                     LGLVGTIFSTIVIALLRTIFGI"
     gene            338..2236
                     /locus_tag="PZ377_002154"
     CDS             338..2236
                     /locus_tag="PZ377_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDM6359016.1"
                     /translation="MGLPNPKTRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPEKST
                     PAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVENLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNM
                     IDNGVAPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTGSAM
                     NVKRIWESNTQCYQMLNLGKYQGISVSALNKILKGKGTLDGQGKAFAEACKKNNINEI
                     YLIAHAFLESGYGTSNFASGRYGAYNYFGIGAFDNDPDYAMKFAKNKGWTSPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGI
                     YFIRDKYK"
     gene            2249..3421
                     /locus_tag="PZ377_002155"
     CDS             2249..3421
                     /locus_tag="PZ377_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDM6359017.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYNIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGEAGKKGEQGAPGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLEDKLQEVEKIKDTTLNDSKTYTDTKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFEKFKQTLNDLYAPKNHNH
                     DERYVLSSQAFTKQQADNLYQLKSASQPTVKIWTGTENEYNYIYQKDPNTLYLIKG"
     gene            3427..3822
                     /locus_tag="PZ377_002156"
     CDS             3427..3822
                     /locus_tag="PZ377_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359018.1"
                     /translation="MEGNFKNVKKFIYEGEEYTKVYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRVSQTNLGSSELGISGVNSGVAS
                     KNINFSNPSGTLYVTISDVYSGSPTLTIE"
     gene            3878..4315
                     /locus_tag="PZ377_002157"
     CDS             3878..4315
                     /locus_tag="PZ377_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDM6359019.1"
                     /translation="MDINWKLRFKNKAVLTGLVGALLLFIKQITDLFGLDLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVSDSSIAQTYQAPRDSSKEEQQVTWKTSQDTSLT
                     PELSTKAPKEYDTSQPFTDTSNEIGFDVNEYHHGGGDNASKIN"
     gene            4296..5741
                     /locus_tag="PZ377_002158"
     CDS             4296..5741
                     /locus_tag="PZ377_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDM6359020.1"
                     /translation="MQAKLTKKEFIEWLKTSEGKQFNADLWYGFQCFDYANAAWKVLF
                     GLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEA
                     TLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSV
                     QSPTQAPKKETAIPQPKAVELKIIKDVVKGYDLPKRGGNPKGIVIHNDAGSKGATAEA
                     YRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQLGNKYYYGIEVCQSM
                     GADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDP
                     VTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKY
                     GTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYT
                     WEGQRYYLPIRTWNGSAPPNQILGDLWGEIS"
     gene            5984..6136
                     /locus_tag="PZ377_002159"
     CDS             5984..6136
                     /locus_tag="PZ377_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359021.1"
                     /translation="MCKEVKSLMLDIIKTLLEHQVLAVLIIPEVLKQLREWHLGYLDR
                     KPNNKD"
     gene            6207..6317
                     /locus_tag="PZ377_002160"
     CDS             6207..6317
                     /locus_tag="PZ377_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359022.1"
                     /translation="MTEQMYLILFLLSLPLLLFIGRKTHFYCLDKKNGRR"
     gene            6319..6504
                     /locus_tag="PZ377_002161"
     CDS             6319..6504
                     /locus_tag="PZ377_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359023.1"
                     /translation="MSDYKLKIIELIKSDITGYQIHKQTGVAQYVISQLRQGKREVDN
                     LTLNTTEKLYSYARQVL"
     gene            complement(6830..6910)
                     /locus_tag="PZ377_002162"
     CDS             complement(6830..6910)
                     /locus_tag="PZ377_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359024.1"
                     /translation="MFLIFSVLQQNGIKNGIICSYFSFTY"
BASE COUNT         2565 a         1084 c         1409 g         1909 t
ORIGIN      
        1 agtcttagag cgcaagaaaa gatttatgac aaattagata gaaattttga agaattaaag
       61 cgcgacaagg tagaagatga aaagaataaa gaaaagaatg ccaagaatat tagagacata
      121 aaaatgtgga ttctcggttt ggtagggact atcttcagta cgatcgtcat agctttacta
      181 agaactattt ttggtattta aaggaggtga ttaccatgct taaagggatt ttaggatata
      241 gcttctgggc gtgcttctgg tttggtaaat gtaaataaca gttaagagtc agtgcttcgg
      301 cactggcttt ttattttgat tgaaatgagg tgcatacatg ggattaccta atccaaagac
      361 tagaaagcct acagctagtg aagtggttga atgggcgtta tatatcgcta aaaacaaaat
      421 agctattgat gtacctggtt ctggaatggg agcacaatgc tgggatttac ctaattattt
      481 actcgataaa tattgggggt ttagaacatg gggaaatgct gatgctatgg ctcaaaaatc
      541 caattataga ggtagagatt tcaagataat tagaaataca aaagattttg taccacaacc
      601 aggcgactgg ggtgtttgga ctggtggttg ggcaggacat gtaaacattg tagtgggacc
      661 atgcacaaaa gactattggt atggcgtaga tcaaaactgg tatacaaata acgcaacagg
      721 aagtccgcca tataaaatta agcactctta tcatgatgga ccaggtggag gggttaaata
      781 ttttgttaga ccaccatatc atccggagaa atctacgccg gcacctaaac ccgaagatga
      841 tagtgatgat aacgaaaaaa ataataaaaa agttccaatt tggaaagatg taacaactat
      901 aaagtacaca atttctagcc aagaagttaa ttatccggaa tatatttatc actttatagt
      961 agagggtaat cgacgactcg aaaaacctaa agggataatg attagaaatg ctcaaacaat
     1021 gagttcagta gaaaatttat ataacagtag gaagaaatac aaacaagatg tggaatatcc
     1081 ccacttttat gttgatagac ataatatttg ggcacctaga agagctgtat ttgaagttcc
     1141 taatgaacct gattatatag ttatagacgt atgtgaagat tatagtgcga gtaaaaatga
     1201 atttattttt aatgagattc acgcaatggt tgtagctgta gatatgatgg ccaaatatga
     1261 gatacctcta agtattgaaa atttaaaagt agacgacagc atttggcgtt ctatgttgga
     1321 acatgttaat tggaatatga ttgacaacgg tgttgcccct aaagataaat acgaagcatt
     1381 agaaaaggca ttacttaata tatttaaaaa cagagaaaaa ttattaaatt ctataactaa
     1441 accaacagta acaaaatcta gaataaaagt tatggtagat aataaaaacg ctgatatagc
     1501 gaatgtaaga gactcatcac caacagctaa caatggctcg gcatctaaac aaccgcagat
     1561 tataactgaa acgagccctt atacattcaa acaagcactg gataaacaaa tggcaagagg
     1621 taacccgaaa aaatctaatg cttggggctg ggctaacgct acacgagctc aaacgggctc
     1681 ggcaatgaat gttaaacgaa tatgggaaag taacacgcag tgctaccaaa tgcttaattt
     1741 aggaaagtat caaggcattt cagttagtgc gcttaacaaa atacttaaag gaaaaggaac
     1801 gctcgacgga caaggcaaag cattcgcgga agcttgtaag aaaaacaaca ttaacgaaat
     1861 ttatttgatc gcgcacgctt tcttagaaag tggatacgga acaagtaact tcgctagtgg
     1921 tagatacggt gcatataatt acttcggtat tggtgcattc gacaacgacc ctgattatgc
     1981 aatgaaattt gctaagaata aaggttggac atctccagca aaagcaatca tgggcggtgc
     2041 tagcttcgta agaaaggatt acatcaataa aggtcaaaac acattgtaca gaatcagatg
     2101 gaatcctaag aatccagcta cgcaccaata cgctactgct atagagtggt gccaacatca
     2161 agctagtaca atagctaagc tatataaaca aatcggctta aaaggtatct attttataag
     2221 agataaatat aaataaagag gtgtataaat gtacaaaata aaagatgttg aaacgagaat
     2281 aaaaaatgat ggtgttgact taggtgacat tggctgtcga ttttacactg aagatgaaaa
     2341 tacagcatct ataagaatag gtatcaatga caaacaaggt cgtatcgatc taaaagcaca
     2401 tggcttaaca cctagattac atttgtttat ggaagatggc tctatattca aaaatgaacc
     2461 tcttattatc gacgatgttg taaaagggtt ccttacctac aatataccta aaaaggttat
     2521 caaacacgct ggttatgttc gttgtaagct gtttttagag aaagaagaag aaaaaataca
     2581 tgtcgcgaac ttttctttca atatcgttga tagtggtatt gaatctgctg tagcaaaaga
     2641 aatcgatgtt aaattggtag atgatgctat tacgagaatc ttaaaagata acgcgacaga
     2701 tttattgagc aaagacttta aagagaaaat agataaagat gtcatttctt acatcgaaaa
     2761 gaatgaaagt agatttaaag gtgcgaaagg tgataaaggc gaaccgggac aacctggtgc
     2821 aaaaggtgaa gcaggtaaaa aaggagaaca aggcgcaccc ggtaaaaacg gtactgtagt
     2881 atcaatcaat cctgacacta aaatgtggca aattgatggt aaagatacag atatcaaagc
     2941 agaacctgag ttattggaca aaatcaatat cgcaaatgtt gaagggttag aagataaatt
     3001 gcaagaagtt gaaaaaatca aagatacaac tctcaacgac tctaaaacgt atacggatac
     3061 aaaaattgct gaactagttg atagcgcgcc tgaatctatg aacacattaa gagaattagc
     3121 agaagcaata caaaacaact ctatttcaga aagtgtattg caacagattg gctcaaaagt
     3181 tagtacagaa gattttgaga aattcaaaca aacactaaat gatttatatg ctccaaaaaa
     3241 tcataatcat gacgagcggt atgttttgtc atctcaagct tttactaaac aacaagcgga
     3301 taatttatat caactaaaaa gcgcatctca accgacggtt aaaatttgga caggaacaga
     3361 aaatgaatat aactatatat atcaaaaaga cccgaatacg ttgtatttaa ttaaagggtg
     3421 atttttatgg aaggtaattt taaaaatgta aagaagttta tttacgaagg tgaagaatat
     3481 acaaaagtat atgctggaaa tatccaagta tggaaaaagc cttcatcttt tgtaataaaa
     3541 cccttaccta aaaataaata tccggatagc atagaagaat caacagcaaa atggacaata
     3601 aatggagttg aacctaataa aagttatcag gtgacaatag aaaatgtacg tagcggtata
     3661 atgagggttt cgcaaactaa tttagggtca agtgaattag gaatatcagg agtcaatagc
     3721 ggagttgcaa gtaaaaatat caactttagt aatccttcag ggacgttgta tgtcactata
     3781 agtgatgttt attcaggatc cccgacattg accattgaat aattttaaac gactaatttt
     3841 ttagtcgttt tttattttgg ataaaaggag caaacaaatg gatattaact ggaaattgag
     3901 attcaaaaat aaagcagtac taactggttt agttggagcg ttgttgctat ttatcaagca
     3961 aatcacggat ttgttcggat tagatttatc cactcaatta aatcaagcta gcgctattat
     4021 aggcgctatc ctcacattac ttacaggtat tggtgttatt actgacccaa cgtcaaaagg
     4081 cgtctcagat tcatctatag cacagacata tcaagcgcct agagatagta gcaaagaaga
     4141 acaacaagtc acttggaaaa cttcacaaga tactagctta acaccggaat taagtacaaa
     4201 agcaccgaaa gagtacgaca catcacaacc atttacagac acctctaatg aaatcggttt
     4261 tgacgtgaac gagtatcatc atggaggtgg cgacaatgca agcaaaatta actaaaaaag
     4321 agtttataga gtggttgaaa acttctgagg gaaaacaatt caatgcggac ttatggtatg
     4381 gatttcaatg ctttgattat gccaatgctg cttggaaagt tttgtttgga ttacttctaa
     4441 aaggtttagg tgcaaaagat ataccatttg caaacaattt cgatggacta gctactgtat
     4501 accaaaatac accagacttt ttggcacaac ccggcgacat ggttgtgttc ggtagtaatt
     4561 acggtgcagg atacggacac gtagcatggg taattgaagc aactttagat tatatcattg
     4621 tatatgagca gaattggcta ggcggtggct ggactgacgg aatcgaacaa cccggctggg
     4681 gttgggaaaa agttacaaga cgacaacatg cttatgattt ccctatgtgg tttatccgtc
     4741 cgaattttaa aagtgagaca gcgccacgat cagttcaatc tcctacacaa gcacctaaaa
     4801 aagaaacagc aattccacaa cctaaagcgg tagaacttaa aattatcaaa gatgtggtta
     4861 aaggttatga ccttcctaaa cgtggtggta atcctaaagg tattgtcatt cataatgacg
     4921 caggaagcaa aggggcgaca gcggaagctt atcgcaacgg attagttaac gcgcctttat
     4981 cgagattaga ggcaggtatt gcacatagtt atgtatcagg taacacagtg tggcaagctt
     5041 tagatgaatc acaagtaggt tggcatactg ctaaccaatt aggcaataaa tattattacg
     5101 gtattgaagt gtgtcaatca atgggagcag ataatgcgac gtttttaaaa aatgaacagg
     5161 cgactttcca agaatgcgct agattgttga aaaaatgggg attaccagca aacagaaata
     5221 caatcagatt gcacaatgaa tttacttcaa catcatgccc tcatagaagt tcggttttac
     5281 acactggttt tgacccagta actcgcggtc tattgccaga agacaagcgg ttgcaactta
     5341 aagactactt tatcaagcag attagggcgt acatggatgg taaaataccg gttgccactg
     5401 tctctaatga gtcaagcgct tcaagtaata cagttaaacc agttgcaagt gcatggaaac
     5461 gtaataaata tggtacttac tacatggaag aaagtgctag attcacaaac ggcaatcaac
     5521 caatcacagt aagaaaagtg gggccattct tatcttgtcc agtgggttat cagttccaac
     5581 ctggtggata ttgtgattat acagaagtga tgttacaaga tggtcatgtt tgggtaggat
     5641 atacatggga ggggcaacgt tattacttgc ctattagaac atggaatggt tctgccccac
     5701 ctaatcagat attaggtgac ttatggggag aaatcagtta gaatgacata gtcatgtcta
     5761 tttgagcagg tgcgttacat acctgctttc tatttacatt taaagataaa atgtgctatt
     5821 attttactag aactttttaa catttctctc aagatttaaa tgtagataac aggcaggtac
     5881 tacggtactt gcctattttt tatgcaaatt taaaaaaaca cttgcttaat aaacaattgt
     5941 ttagtataat tatatttgta ggttagttga tgacttacaa attatgtgta aggaggtgaa
     6001 aagcctcatg ctagacataa taaaaacact tctagaacat caagtattgg cagtactgat
     6061 aattccagaa gtgttaaaac aacttagaga atggcatctc ggctacctag accgaaagcc
     6121 aaacaacaaa gattaacatt atgcttggag cctgatggct cctccttaca cttatataat
     6181 ataatattat ttggaggttt tcaattatga cagaacaaat gtatttaata ttgtttttat
     6241 taagcctacc attgttatta tttatcggga gaaaaacaca tttttattgt ttagataaaa
     6301 agaatggacg tagataatat gagtgattat aaattaaaaa taattgaatt gatcaaaagt
     6361 gatataacag gttaccaaat tcacaaacaa actggcgtag cgcaatatgt aatttcacaa
     6421 ttaaggcaag gaaagcgcga agtagataac ttaactttaa atacaactga aaaactatac
     6481 agttacgcac gacaagtgtt ataatataaa tgtgaaatgg tcattcttga aatgactcgg
     6541 tcggtactgg cacagaccgt ttaaagtgtc accacaacat gaactgagaa ttcatatgac
     6601 gttgctgacg agcgacaaag ctctgtgttc ctgaatggga gtaggtttgt gtggtggtat
     6661 aatttagtaa cagcatagac tgtctatagc aaagttgccg aagagattct aaacgtattt
     6721 ataaatacgt ggcccttgct agataaccgc atcttaactg atgcggttat ttttatcccc
     6781 acacaaccaa caaaaccaca ccacctatta atttaggagt gtggttgttt taatatgtga
     6841 agctaaaata actacaaatg ataccatttt tgataccatt ttgttgtaaa acagaaaaaa
     6901 taaggaaaat aaaaaaggca aaaaacgcat taaatcaacg tttattgtct catgaaattt
     6961 aaatgta
//
LOCUS       DANRDI010000132         6964 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.132, whole genome shotgun sequence.
ACCESSION   DANRDI010000132 DANRDI010000000
VERSION     DANRDI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.132"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            55..315
                     /locus_tag="PZ377_002163"
     CDS             55..315
                     /locus_tag="PZ377_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDM6359025.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            355..615
                     /locus_tag="PZ377_002164"
     CDS             355..615
                     /locus_tag="PZ377_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDM6359026.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            624..887
                     /locus_tag="PZ377_002165"
     CDS             624..887
                     /locus_tag="PZ377_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359027.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            896..2839
                     /locus_tag="PZ377_002166"
     CDS             896..2839
                     /locus_tag="PZ377_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDM6359028.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            2841..3761
                     /locus_tag="PZ377_002167"
     CDS             2841..3761
                     /locus_tag="PZ377_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDM6359029.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            3842..4459
                     /locus_tag="PZ377_002168"
     CDS             3842..4459
                     /locus_tag="PZ377_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDM6359030.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            4460..4930
                     /gene="ssb"
                     /locus_tag="PZ377_002169"
     CDS             4460..4930
                     /gene="ssb"
                     /locus_tag="PZ377_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDM6359031.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            4960..5853
                     /locus_tag="PZ377_002170"
     CDS             4960..5853
                     /locus_tag="PZ377_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDM6359032.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            5860..6078
                     /locus_tag="PZ377_002171"
     CDS             5860..6078
                     /locus_tag="PZ377_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359033.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6087..6491
                     /locus_tag="PZ377_002172"
     CDS             6087..6491
                     /locus_tag="PZ377_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDM6359034.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            6504..6872
                     /locus_tag="PZ377_002173"
     CDS             6504..6872
                     /locus_tag="PZ377_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359035.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            6876..>6964
                     /locus_tag="PZ377_002174"
     CDS             6876..>6964
                     /locus_tag="PZ377_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359036.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAH"
BASE COUNT         2907 a         1050 c         1254 g         1753 t
ORIGIN      
        1 attaacaaaa ttaattcatt ttcaatataa aacgaaacaa ggaggaagtc aaccatgact
       61 aaaaattata aagatatgac tcaagacgaa ttaaggggtt tattggctga aaagaatgca
      121 gaattgtttg atttagcgag cgaaatcgat gaagaaactg aatttgacgt tttacttttt
      181 tcaaatgtag ggattagcaa cggagatttt acaccgtcgt cacattgtgt gattgggaat
      241 gttgttgata ttgctaattt attgaaacgt agagctgttt accgagatat cgctgatgtt
      301 atcaaaatgc gttaaaaatt ctagctatag ataatgacaa ggaggacgag aacaatgtat
      361 tacaaaacgg gtgacgtatg tcgaaaaata attaatgtag atggctttga ttttcaatta
      421 agagttaaga aacgagcgta tagcgtcgaa atggttgttc tagaccatga aggaaattca
      481 attgacgggt tactagtttc tgatgagaac gatctataca cagctttaga tattttgaaa
      541 caaagtgttt atgaatggat tgaaaataac acagatgaac aggacaaact aatgaactta
      601 gtcatgaaat ggtaggtata agcatgagag atacagaaag aaatatattg aatattttta
      661 agacgttatt cgacgaatat actttgtcaa accaacgagc attattggaa attgaacgta
      721 atcatcacgg atacttatcg attaatttct tgcactatca cgacagttac aaaacaaaca
      781 ataagcttgt gcagatacat gaaatcaatc cagacagcca tgaacgaata aaaaatttaa
      841 ttatcgaggt actaagaggt catcggaaga ttaaaaaagg agcatgagga aggatatgaa
      901 aataaataaa ttaacaatat cgaactttgc cggaatcaaa gaagaaaaat ttaattttga
      961 cggtaaagat gcaaaaatat acggcaataa tgcgactggt aagactacaa cagcaaccgc
     1021 attacaatgg ttgcttttcg ataaaggttt ggacggttca accaaatcat ttaaccctgt
     1081 acctttaaac gaaaaaaacg aagaaaatta tgagttaatt ccgactgttt tcgcagaatt
     1141 tgaaatcgac ggaaaaatta cgacttttaa aaaagagtca catcctaaat acacaataaa
     1201 tcaaaagacg aatcgcaagg aatactcacg aagtcgaacg aagaaacaat atatcaatga
     1261 tgaatcaata aaagtaaagg attataaagc tcgtattgat gaacttattg atgaagatgt
     1321 attcaagtta attacgaacc ctcaagcatt taacttacta gattggaaga agcgaagaag
     1381 tttgttgttt gaaattgcta aaccaatcaa tgatgaggat gtcattaaaa caaatgatga
     1441 ttttaaagaa ctaaataata ttcttggaga tcatgaaatt gaaacaaaga aaaagattct
     1501 tacggacaag ataaaacaga ttaacaaaga tatcaaagat attccgatac gtattaacca
     1561 aacacaacaa aataagcagg atgtaccaga attcgataat gatagatacg caattatcaa
     1621 acaagaaatt gagcaacttg aaaatgagcg tatagatatt caaaacggta aggaagaaat
     1681 taatttacgt aatcaattag ctgataaaca atcagaattg aaacgcatag aagacaataa
     1741 cagcgcaagt aatgagaaca aaatccatgc tttaacaaat gaattacacg ttgaaaatgg
     1801 aacggtagca aaccttaaaa cgagattaaa gcaaaacaaa caacaaatca cgcatgaaga
     1861 aaatagacgt aatcaattat tagaaaatca taaaggatta aaaagtgatt tagaaaaagc
     1921 taaaaatcaa aaatttgaat atcttgatga caatgtatgt agttgttgtg gtcaaaagtt
     1981 accagttgaa caagtggata tggcaagaga aaaagcattg cagaaattca atgctagcaa
     2041 atcgaaagaa ttagaaacaa tacaaacatc tatcaatcac attatttcag aaggcaagaa
     2101 aataaagcta atcatcgaga agttagagga tgacaataat aatcttcaaa ttaaaatcaa
     2161 cgaagcagaa gagcgttcag caagaataca aaacaaaatt aataagttga aaacaactca
     2221 cgttgacgtt acgcaaactg acgaatacaa agcagtaatg ttagagataa atgagattaa
     2281 tcaaaaacgc tctaacatca ggaaaactat tcaagataaa gtttcaggaa tagatgacaa
     2341 aataagcgaa cttactcaag aaaaatcaga aattgaagtg tcaagatcaa tcgaaaaatc
     2401 aaataaacat ctagatgatg ttatttctga attaagaaat gaagaagaca gattattgga
     2461 tgaaaaagaa aagtattcac atgaccttta tatcttaaaa gaatttacaa caacaaaagt
     2521 caaaatgctt actgaaaaca tcaataatga atttgaaatt gctgaattta agctattcaa
     2581 taccttagtt aacggcgaat tagaagaaac atgttccaca acggttaacg gcgtcgaata
     2641 cgacagcggt ttaaataacg cctcaagaat taatgttggc ttagatatca tcaatacact
     2701 gtcaaaacat tttaaagtta cagcgccaat atttattgat aatgctgaat cagtaacaga
     2761 gcttatcaaa acagaatcac aacaaattca attgatagta aatgaacaag ataaaaaatt
     2821 aagaatggag actatataaa atgactgaaa ataataaatt acaaactatt gaacaacaat
     2881 tagtacaaga aaagaacgta tctgacaacg tattaaacaa agtgagagtt ttagagtcac
     2941 aaggcaattt ggaattgcca aatgattatt caccaagtaa tgccatgaaa caagcatggt
     3001 tacaaatcag ccaagataac aaattaatga gttgtaacga tacaagcaaa gcaaatgcct
     3061 tattagacat ggtaacgcaa ggtttaaatc cagctaaaaa tcaatgctac tttattcctt
     3121 acggcaacaa aatgcagtta caacgtagct atcacggtaa tgtaatgatg ttaaaacgtg
     3181 atgcaggtgc tcaagatgtt gttgctcaag tgatttataa aggcgataca ttcaagcaag
     3241 aaatgggaga aacaggacgt atcaaagcga ttaaacacga acaagacttc tttaacatcg
     3301 acaaagaaaa cattatcggt gcgtactgca caatcgtatt taatgatgga cgagataact
     3361 atattgaagt catgactatt gaacaaatta aacaagcatg gatgcagtca tcaatgatta
     3421 aagatgaaaa agcattacaa aattctaaaa cacataataa tttcaaagaa gaaatggcta
     3481 aaaaaacagt tatcaataga gctgctaaac gttatatcaa cacatcaaca gatagcaatc
     3541 ttttcaaata cgcacaagaa tccgaacaac gtcaacgcaa agaagtgttg gacgcagaag
     3601 ttgaagaaaa tgcaaatcaa gaacaattgg actttgaaca accagttctt gaagaagcac
     3661 aatacacaga attagaaaat gataagccta ttgatgtatc tgactttgaa gaaataaaag
     3721 aacctgcaac agaaaaagaa agcgaagaag agccatttta attgaaacaa tagcaactgg
     3781 ttcaagtggt aactgctacg tcttaaatga tggacgtact acgttactac ttgaggcagg
     3841 tataaaattt gaacgtgttc aaaagcattt caaatataaa acaagacata tagcagggtg
     3901 tcttatcaca cacgaacatg gtgatcatgc aaagtataca aagcagtttg tcgacaatgg
     3961 tgtaatcagc tatatgactg ctggaacaca acaagctatg aattttgaaa gtcatcgctt
     4021 atgcacgatt aaggcaaagc aagagctgcg aataggtaca tggtcaattc taccgtttga
     4081 catcgaacat gatgctaacg agcctgtggc tttcttatta caaagcacac taggttataa
     4141 ggttctgtat gttactgata caaagtattt gaaatacaaa tttaatggca ttacgcacat
     4201 gatgctagaa gttaattata tctatgaaca aatgcaagaa aacataaaaa acggcagtgt
     4261 gcacagcaca ttagcaaaca gaattatgga gtctcatttt agcttagaac atgctatcgg
     4321 aatgttaaaa gcaaatgatt taactagact cgaagaaata catttaattc atttaagtag
     4381 tcaaaattca aatgcaaaat acattaaaag tgaaatacaa aaagtgacgg gcgcgcccgt
     4441 ttattttgga ggtttataaa tgctaaacag aacaatatta gttggtcgtt taactagaga
     4501 cccagaatta agaaccactc aaaatggtgt aaatgtagca tcattcacat tagcagttaa
     4561 ccgtacattt acaaatgcac aaggcgagcg cgaggcagac tttattaata tcatcgtatt
     4621 taaaaaacaa gcagagaacg ttaataaata cctatctaaa ggatcgttgg cgggcgtaga
     4681 tggtaggtta caaacgcgga actatgaaaa taaggaaggt caacgtgtat acgttacgga
     4741 agttattgct gatagtattc aatttttaga accgaaaaac tcaaatgaca ctcaacaaga
     4801 tttatatcaa caacaagtac aacaaacacg tggacaatcg caatattcaa ataacaaacc
     4861 agtaaaagat aatccgtttg cgaatgcaaa tggtccgatt gaactaaatg atgatgattt
     4921 accattctaa tttaaccggt ttgaaagtga ggtgtgtata tgactggttg gataagtatt
     4981 gatcgctcaa ttcaaaatca ttggctattt aaagaaaaga gaacattttc aaagtttgaa
     5041 gcatggatat atttactcat ggaagcgaat cattcaaagg caaaagtgcc tataggaaac
     5101 caaattgtaa ccgttgaaag aggacaaaga ttaacatcta ttttgacctt gtctgacctt
     5161 tttaactggt cacgatttaa agtgaaaacc ttccttgact tactcgagag tgatggaatg
     5221 ttagaagtca aaacaacatc aaaatatacc cttataacca ttgtcaatta tgacttttat
     5281 caaagtgagc agggcaggaa ccaacatcaa aacgacatca aaccaacatc aaaacaacat
     5341 cagtcaaaca tcaacccaac atcaaaacaa catcaaacca acacaaacaa taatgataat
     5401 aaagataata atgaaaagaa tgtgaataat gagaagaaga agacaaccgc cttcgacttc
     5461 ttccaagata acggattcgg tttcataact tcttacaatt tagacgattt aaattattat
     5521 cttgattcat ttgaaaatga ttcagatgaa atagttaccg catcacttaa aatcgctaaa
     5581 gacagaaaca aagttacttg gggatatgct aaaagcattt tgaatacatg gcttaatgca
     5641 aacttgaaat ctattgaaca agtacgtgca tttgaaaagc aacaacttga aagcaaaaaa
     5701 caaaattata aacctttcgt taaacaatca aaagaaaaaa cacccaaatg gctcacagac
     5761 agcacgagag aaacgaaaac gccggaagta gatgaaaacc ttgagaaaga cagagaagct
     5821 tttattaagc gtctaaatag caaatgggag tgattgaaaa tggatgcatt tgataaatac
     5881 tatctatttg atcatgacgg caacaaaatg ttttcagtta caccacattt taaagatgga
     5941 cggcatttag ttgttggatt aaaacacaca aaattcaatg gtcgacgttg gtacttagat
     6001 gattatgaat taaaaacact tattgataat gaacaaatgg agttaggaca ccaaacaagc
     6061 ttatttgaat atatatgagg gattacatgg agatagaaat taaatttaat gaagtgttta
     6121 atgcgccgat gggctcgcct cgaccgcgtt ttagcacaaa aggtagatat gcacacacat
     6181 atatgcctac aaaatataca gaacataaaa aatatttaca aaatcaaatg ccaaagctaa
     6241 atctagaaaa tgcattaaaa attgaattag agttttactt tactccacct aaatcatggt
     6301 cgaagaaaaa gaaaactcaa gcgattggac aattaaaagt aactaaacca gatatcgata
     6361 atttgatgaa gacagttcta gacgcttgta acaattattt gtggaaagac gataaccaaa
     6421 ttacagaaat aactagctca aagcgttatg gaattgagcc caaaataatc atacgaatag
     6481 aagaaatata agaggtggat aaaatggcga gaaaagcaag gattgtaaca ataaatgata
     6541 aaccatatag gtttaccaaa tctgaaatgg aattaataga aagtcacggt ataaccgctg
     6601 gaatggtttc taagagagta aaagacggtt gggaactaca tgaagcaatg gacgcaccag
     6661 aaggtacgcg tttaagcgag tacagagaaa agaaaacaat agaaagactg gaacaagcta
     6721 gactcgaacg caaattggaa agaaagcgaa agaaagaggc tgagctaaga agaaagaagc
     6781 cacatttgtt taatgtacca cagaaacatc caagaggacg ttatgcgtgc tacctgatgg
     6841 aaaacgacat attcgtgaaa gttaagaagt agatcatgac agataacgca cgcaaagaat
     6901 acctaaatca attctttgga tctaagagat atctgtatca ggataacgaa cgagtggcac
     6961 atat
//
LOCUS       DANRDI010000133         6947 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.133, whole genome shotgun sequence.
ACCESSION   DANRDI010000133 DANRDI010000000
VERSION     DANRDI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.133"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..405)
                     /locus_tag="PZ377_002175"
     CDS             complement(<1..405)
                     /locus_tag="PZ377_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HDM6359037.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVC"
     gene            587..1948
                     /locus_tag="PZ377_002176"
     CDS             587..1948
                     /locus_tag="PZ377_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDM6359038.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            1935..3608
                     /gene="menD"
                     /locus_tag="PZ377_002177"
     CDS             1935..3608
                     /gene="menD"
                     /locus_tag="PZ377_002177"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDM6359039.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            3595..4398
                     /gene="menH"
                     /locus_tag="PZ377_002178"
     CDS             3595..4398
                     /gene="menH"
                     /locus_tag="PZ377_002178"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDM6359040.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            4391..5212
                     /gene="menB"
                     /locus_tag="PZ377_002179"
     CDS             4391..5212
                     /gene="menB"
                     /locus_tag="PZ377_002179"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDM6359041.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(5451..5780)
                     /gene="sspC"
                     /locus_tag="PZ377_002180"
     CDS             complement(5451..5780)
                     /gene="sspC"
                     /locus_tag="PZ377_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDM6359042.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(5818..6942)
                     /gene="sspB"
                     /locus_tag="PZ377_002181"
     CDS             complement(5818..6942)
                     /gene="sspB"
                     /locus_tag="PZ377_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDM6359043.1"
                     /translation="MLILSLGAFANNNKAKADSHSKQLEINVKSDKVPQKVKDLAQQQ
                     FAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYYYPVIKDGKIVYTLTLSPKN
                     KDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLTDEKGFYFEEDGKVRLVKAT
                     PLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQEDQVQYENTLKNFKIREQQ
                     FDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSEQDLPNCATFPNQMIEYGKS
                     QGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPNDPHLGHALAVVGNAKINDQ
                     EKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIGY"
BASE COUNT         2379 a         1007 c         1283 g         2278 t
ORIGIN      
        1 acatactaat ccaactggta ataaccaaaa tgaagagtta gcagctaaaa acaaacctaa
       61 tattgctgct aagatgtaaa atgcaatggc taatcgtagc acaagctctg ggctcatacc
      121 gttgcgaaca atggcaccac caatgcctac agattcatga tcatcaaggc cttttttata
      181 atcatagtat tcattaaaca tattagttgc tgcttgaata agtaagcatg ctagtaacat
      241 ggcaatgaat aggctgattt taatatgatc ttcgctacca agaaaatata ttttagatgc
      301 tgctgtacca actaaaacgg gtactacgga agcagttaat gtatgaggac gcattaaatg
      361 ccaatatttc ttaactgtag aatattgctg atattgatta ctcattgaat acaaaacctc
      421 tttaaatcat attttttata acaaagatat tttaatagaa agtagcataa aggtcaattg
      481 acgtgaaaaa ttgcaagata agtttaagca attcttgtaa cacaattatc caaaaatgat
      541 acaatataat aatgagtgaa aaaagacgaa gaaagaagtg aaatggatgg ctacgggcgt
      601 attagaggac gatattgtca aagagatata tggcagctca aaggaatggg tttcagttga
      661 agtgaaatta tcacagtcac tagacccgag cacattattt catctcactg acaatgaggc
      721 aggagatcgc ttttatatgc gtttgaatga taatcgaacg tcatattttg gctacaaagc
      781 aattcaatta ttcaaaaata attctaaaaa taaacaatct atttttaaag actgggaaaa
      841 attaaaacat aacatcacat ttatacatcc gcaatctgag aaacatcatc ttcgagttgt
      901 tggagggttc caattttcaa gtcataaatc agatgatgaa tggcgagagt ttggactaaa
      961 tcattttgta ttacctgaag ttttaatttc aactgataat aatgggacat ttttaactta
     1021 tacagttaaa agggaaagtt ttactgttga ggcattgaac gatttaatgg atttgttcaa
     1081 caatatatcg gacatagatg tggacgagca aattggggga attactagaa atgaagatat
     1141 ttataaagat gactggcgtc aacttgtagt agaagctata gaatctatta ataatgaaga
     1201 aaaaattgta ctagcacgta gacggttaat aaagttcgat aaagatatca gtattccata
     1261 tattctaaag caagcatatt ctaaagaaaa aaacagttat atattcttgt tagaatcaca
     1321 agattctata ttcttttcac aaacacctga acaattaata aaggtcaata ataaaatact
     1381 atcgactaaa gctgtagcag gtacaattaa acgttcacaa gatgaggacg aagatacaaa
     1441 aaatgttgaa gcatttttaa aagataataa aaacttaatc gaacatcgat ttgttgttga
     1501 cagtatttta catgatatta aaccttatat cactgaatta cattatgata agacgcctaa
     1561 aattctaaaa aatgatcatt tatatcactt gtacactgaa ataaaggcgc cactgaagga
     1621 tgattcgtat attagtttaa ttgataattt acatccaaca cctgctttag gtggctatcc
     1681 aaaagaattt gcaatggatt ttattgagca gaaagaattt ggtacgcgag gattatatgg
     1741 tgcaccggtt ggctatatag atatatacga tgattgtgaa tttattgttg caattcgttc
     1801 gatgcttatt aagaaagcac aagcaacttt atttgctggg tgtggcattg ttaaagattc
     1861 tgatccagat agtgaattgg cagaaacgaa ccttaagttc acacctatga tgaatgcatt
     1921 aggagtcgat atgaatggga aatcataaag cagctttaac gaagcaagtt tttacatttg
     1981 catctgagtt atatgcgtac ggcgtaaggg aagtagttat cagtccggga tcacgctcaa
     2041 cgccacttgc acttgcattt gaagcacatc caaatattaa aacatggata caccccgatg
     2101 agcgaagtgc agcgtttttt gcagttgggt taattaaagg cagtgaaaga cctgtcgcta
     2161 tattatgtac gtcaggtaca gcagcagcga attatacgcc tgcaattgct gaaagccaaa
     2221 ttagtagaat tcctttaatc gttttaacaa gtgaccgtcc gcatgaatta agaagtgtag
     2281 gcgcaccaca agcgattaat caagtaaata tgtttaataa ttatgtaagt tatgagttcg
     2341 atatgcctat tgcggatgat agtaaagaga ccattgatgc aatttactat caaatgcaaa
     2401 ttgctagtca atatttatat ggaccacata aagggccaat tcattttaac ttgccattta
     2461 gagatccgtt aacacctgat ttgaatgcaa cagaattgtt aacttctgag atgaagattt
     2521 taccgcacta tcaaaaaagt atagatgcat cggcattaag acacatttta aataagaaaa
     2581 aaggtttaat tattgtaggg gatatgcagc accaagaagt tgatcaaata ctaacgtatt
     2641 caacgatata tgatttgcct attttagctg atcctttaag tcatttaaga aaatttgatc
     2701 atccgaatgt tatctgtaca tatgatttgc tgtttagaag cggcttagac ttaaatgtgg
     2761 atttcgtaat tcgtgttggg aaaccagtga tttctaaaaa gttaaatcaa tggttaaaga
     2821 aaactgatgc atttcaaata ttagtgcaaa acaatgataa gattgatgtc tttccgatag
     2881 cgccagatat ttcatatgag atttctgcga atgatttctt taggtcatta atggaagaca
     2941 cgacagttaa tcgcgtaagt tggttagaaa aatggcaatg cttagagaaa aaagggcgta
     3001 aagaaattaa atgttatttg gaacaagcta cagatgagag tgcattcgtt ggtgaattga
     3061 ttaagaaaac atctgaaaaa gatgcattat ttattagtaa tagtatgcct atcagagatg
     3121 tagataactt gttattgaat aaaaatatag atgtctatgc gaatcgtggt gcgaatggta
     3181 ttgatggtat cgtttcaact gcactgggta tggctgtgca taaacgaata acattattga
     3241 taggtgattt atcattttat catgatatga atggactatt aatgtcaaaa ttaaataata
     3301 ttcagatgaa tattgtatta ttgaacaacg atggtggcgg tattttttca tatttaccac
     3361 aaaaagaaag tgcaactgac tattttgaac ggttgtttgg cacaccgacg ggattggatt
     3421 tcgagtatac agctaagtta tatcaattcg attttaaacg ttttaacagt gtttcagaat
     3481 ttaaaaatgc cacattgtta tctgaaactt cgacgattta tgaattgata acgaatcgcg
     3541 aagataactt taaacagcat caaattttat accagaaatt gagtgaaatg attcatgaca
     3601 cattataaat tttatgaagc aaacgttgag accaatcaag ttttagtatt tctgcatgga
     3661 tttcttagcg acagccgtac ttatcataat cacatcgaaa aatttactga taactatcat
     3721 gtcatcacta tagacttacc aggccatggc gaagatcagt cttcaatgga tgaaacgtgg
     3781 aattttgatt atattacgac gttgttagac cgaattttag ataaatataa agataaatca
     3841 ataacattgt ttggatattc aatgggtggg cgtgttgcat tatattatgc aattaatggt
     3901 cacatcccta tatctaattt gatattagaa agtacgtcac caggtattaa agaagaagca
     3961 aatcaattgg aacgccgtct tgttgatgat gcacgtgcta aagtattaga catagcaggt
     4021 attgaattat ttgttaatga ttgggaaaag ttgccattat ttcaatcgca actagaatta
     4081 ccagttgaaa tacaacatca aataagacaa caacgattgt ctcaatcgcc acataaaatg
     4141 gccaaagcat taagagatta tggtacaggt caaatgccaa acttatggcc gcgcctgaaa
     4201 gaaattaaag taccaacatt aatattagct ggagaatatg atgaaaaatt tgtacagatt
     4261 gcgaaaaaaa tggcaaattt aattcctaat agtaaatgta aattaatttc tgctacaggt
     4321 catacaattc atgtggaaga tagtgatgaa tttgatacaa tgatattagg atttttaaag
     4381 gaggagcaaa atgactaaca gacaatggga aacacttaga gaatatgatg aaatcaaata
     4441 tgaattttac gaagggattg ctaaggtaac aataaatcgc cctgaagtac gcaatgcgtt
     4501 tacacctaaa acagttgctg aaatgattga cgcattttca cgtgcacgtg atgatcaaaa
     4561 cgtttcagtt atcgtattaa ctggtgaagg tgatttagca ttctgttctg gtggtgacca
     4621 gaagaaacgt ggacatggtg gttatgtagg tgaagaccaa atccctcgct taaatgtatt
     4681 agatttacag cgtttaattc gtattattcc aaaaccggtt atcgcgatgg taaaaggtta
     4741 tgctgtaggt ggcggtaatg tactaaatgt tgtttgtgac ttaacgattg ctgctgataa
     4801 tgctattttt ggacaaactg gtcctaaagt aggttcattt gatgcgggtt atggttcagg
     4861 atatttagca cgtatcgttg gacataagaa agcacgtgaa atttggtact tatgtcgtca
     4921 atacaatgca caagaagctt tagatatggg tctagtaaat acagtggtac ctttagagaa
     4981 agttgaagat gaaactgtgc aatggtgtaa agagattatg aaacactcac caacagcgtt
     5041 acgattcctt aaagcagcta tgaatgctga cacagatggt ttagctggtt tacaacaaat
     5101 ggctggggat gcaacattgc tttattacac aactgatgaa gcgaaagaag gccgtgatgc
     5161 gtttaaagaa aaacgtgatc ctgacttcga tcaattccct aaattcccat aagttatttt
     5221 gaatatgata ttaagtcact tgcgtcgttt attagcgacg ataggtggct tttttatttt
     5281 ataagaattt aaaagggcat tttaattaat tatcataaag ataagatagt ttgagaaaaa
     5341 ttaatagaga aagaataaat gttaaagatt attattacaa tgaaaaaacg agctggtgag
     5401 aaattgaatt ctcgccaacc cgctttatga agtgaaatca tttaaattag ttatactaag
     5461 cgctcataaa cgattggtcg cgaagtgcca ataccttgtt tggatgacgt ttgcatgagg
     5521 atatgctgtg tatcatcata atcaagaata taaatgattt cttcattatc aatagatgaa
     5581 aatttaatgc gttgatgtgc cgtatctata aaaagaatat gatattgatt gtgacttgta
     5641 tcatcgccat gacgtataca aattgatttt tgtaattgat gattactcca attaccaatg
     5701 aataaattga tattggtttg ttctagatgt gtgagtttgg ttgtgtcgta aactaaattt
     5761 ataaattgta gttgatacat agttttacct gcctttaatc aatatctata ttacttttta
     5821 gtaacctatc attgaaccat accagttata atcacgattg aatgataaat gtaataggct
     5881 tgaatctgca tcttggattg ataattctgt atcccaagga ttccagtaaa taagtttttc
     5941 ttggtcatta attttagcat taccaacaac tgctagcgca tgtcctaaat gtgggtcatt
     6001 agggttttga gatacacttt gtgcaaggat cataattcct acattatctt ttgtaagttg
     6061 atcaacttgt tcatatgatg gtacgccttc ttgataatga atatctctgc cttgtgattt
     6121 accgtattca atcatttgat tagggaatgt tgcacaatta ggaaggtctt gctcacttac
     6181 ttcagggtat aatgtacgca taatatcatg tgcattataa gtgtctgtat ttttagttgc
     6241 atttaataat gctgccatac tgaatcctgc acaccatgag ttatcgaatt gttgttctct
     6301 aattttgaag ttttttaatg tattttcata ttgaacttga tcttcttgta tcgcttcgtt
     6361 ttcttcaact ttagtaggtg ttactgtatt ttttaactct tgtttcaatt ttgctgaaac
     6421 tgttttagca ctttcttttt cttttacatt accaggtaga ggcgtagctt ttactaatct
     6481 aactttgcca tcttcttcaa aataaaaccc tttttcatca gtaagaacag tgatatttga
     6541 atttttatct ttaatttggt ctaaatcttt agcgatgaag tttgaaattt taacactgta
     6601 attcatgtct tcttttgatt tgtttaaatc atctttattt ttaggactaa gtgttaaagt
     6661 ataaacaatt ttaccgtctt ttataactgg ataatagtaa ctattatctt cttcaccatt
     6721 aaatttataa attttaaaag cttcgccaag ttcatattta ccagtttttg cattactttg
     6781 tttatctaat gctttagcat aaccagcaaa ttgttgttgt gctagatctt ttactttttg
     6841 aggtactttg tcactcttaa cattaatttc tagctgttta gagtgtgaat cggctttggc
     6901 tttattgtta ttagcaaatg cgcctagtga taaaataagc attgaaa
//
LOCUS       DANRDI010000134         6942 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.134, whole genome shotgun sequence.
ACCESSION   DANRDI010000134 DANRDI010000000
VERSION     DANRDI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.134"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(338..1582)
                     /locus_tag="PZ377_002182"
     CDS             complement(338..1582)
                     /locus_tag="PZ377_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDM6359044.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(1691..2881)
                     /locus_tag="PZ377_002183"
     CDS             complement(1691..2881)
                     /locus_tag="PZ377_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDM6359045.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(3189..4316)
                     /locus_tag="PZ377_002184"
     CDS             complement(3189..4316)
                     /locus_tag="PZ377_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDM6359046.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(4679..5056)
                     /locus_tag="PZ377_002185"
     CDS             complement(4679..5056)
                     /locus_tag="PZ377_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDM6359047.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(5472..6065)
                     /locus_tag="PZ377_002186"
     CDS             complement(5472..6065)
                     /locus_tag="PZ377_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDM6359048.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            6129..6512
                     /locus_tag="PZ377_002187"
     CDS             6129..6512
                     /locus_tag="PZ377_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDM6359049.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            6643..>6942
                     /locus_tag="PZ377_002188"
     CDS             6643..>6942
                     /locus_tag="PZ377_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HDM6359050.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIG"
BASE COUNT         2125 a         1357 c         1007 g         2453 t
ORIGIN      
        1 tattgtaagc tgacttttcg ccagcttctg tgttggggcc ccgccaactt gcattgtctg
       61 tagaaattga ggagctaatt tctctgtgtc ggggctccac cccaacttgc acactattgt
      121 aagctgactt tccgccagcc tctgtgttgg ggccccgcca acttgcacac tattgtaagc
      181 tgactttcca ccagcctctg tgttggggcc ccgactattt ttgaaaagag cgtgttacac
      241 gggcattgtt ttacagtcaa ctactgctaa aataaaatta acgagcttag ggctttgttt
      301 tctgtcccaa gctcgttaaa tcacatatga taattaatta tgcccaacca cgatatctag
      361 ctgcttctgc tgtacgttta atacctatga tatatgctgc aagtctcata tctatttttc
      421 ggttttgaga caattcgtaa atcgtatcaa atgccgcttc taatttttca cgtagctttt
      481 catcaacttc ttcttcagac caataataac cttgattatt ttgtacccat tcgaagtaag
      541 aaaccgttac accaccagca cttgctaata cgtctggaac taataatata ccacgttcag
      601 ttaaaatacg tgttgcttct ggtgttgtag gtccattagc agcttcaaca acgatactag
      661 ctttaatatc atgtgcattg tcttctgtaa tttggtttga aatagccgct ggtactaaaa
      721 tgtcacaatc taattcaaac aattctttat ttgagattgt ttcttcaaat aaatttgtta
      781 ccgtaccaaa actatcacga cggtctaata aataatctat atctaagcca tttggatcgt
      841 gtaatgcacc gtaagcatca gagataccta caatttttgc acctaaatca tataagaatt
      901 tagctaagaa acttccggca ttaccgaaac cttgaataac aaccttggca ccttcaattt
      961 gcatattacg acgttttgca gcttgttcaa ttgcaataac tacacctagt gcagttgatc
     1021 tgtcgcgtcc atgagaacca cccaatacaa ttggtttacc tgtgatgaaa cctggtgaat
     1081 taaatttatc taatgcacta tattcatcca tcatccaagc cataatttgt gagtttgtaa
     1141 atacatctgg tgctggaata tctttgttcg gacctacgaa ttgtgaaatt gctcttacat
     1201 atccgcgtga taaacgttca acttcatgaa tgctcatttg acgtggatca caaacgatac
     1261 cacccttacc accaccgtat ggtaagttta caatgccaca tttcaaagtc atccacattg
     1321 ataatgcttt tacttcttct tcatcaacat ctgggtggaa acgcacgccc ccttttgttg
     1381 gtccaacagc atcattatgt tgcgcacggt aacctgtgaa tgtttttact gtgccatcat
     1441 ccattcgtac agggatacgc acttgtaaca ttcttaaagg ttctttaatt aaatcgtaca
     1501 ttccttcgtc aaatcccaat ttatgcaatg cttctttaat aattccttga gtagaagtta
     1561 ctaaattatt gttctcagtc atgatccttt tcgcctcttc tttacctaat gatttcgctt
     1621 tcaaacatat tgtaacataa cgtattcctt tttaaagccc ttacaaactg attgttacaa
     1681 ctttttgaca ttattgaaat acatgtctta ttttttcaag tgcaaggtcc aattcttctt
     1741 tagtaataat taatggtggt gcaaaacgaa tgacagtatc atgcgtttct ttacataata
     1801 agccttcttc tttcaaagct tcacaatatg gtctagcact ttcattaagt tccacaccta
     1861 taaacaaacc acgtccacgg acttctttaa ttgatggatg atcaatttgc tttaattgtt
     1921 ctttaaaata atctcctaat tctaaagagc ggcctggtaa atcctcatca acgataacat
     1981 ctaatgcagc aattgatgca gcacaagcaa gtggattacc accaaatgtt gaaccatgtg
     2041 agccaggtgt aaagacatct aatacttctt tatctgctaa tacaacagaa attgggaaga
     2101 ctccaccacc tagtgcttta cctaaaatat agacatcagg ttttacatta tcccaatccg
     2161 tagcaaataa tttacccgaa cgacctaatc ctgcttggat ttcgtcagca ataaataaga
     2221 cattatgttc atcacataat tctctaattg ctttcaaata tccttctggc ggtatattta
     2281 tacccgcttc accttgaatt ggttctacta aaactgctgc agtattttca ttaattgcag
     2341 ctttcaatgc atctacatct ccaaaatcaa cttttctaaa tccatctaat aacggaccat
     2401 aaccacgttg gtattctgct tctgaagata atgaaactgg cgccattgtt cgaccatgga
     2461 agttaccatt aaatgcaatg atttctgctt tatttggctc aattccttta acatcgtatg
     2521 cccagcgtcg tgctgctttc aaagctgttt ctactgcttc agcacctgta ttcattggta
     2581 aagctttatc tttacctgcc agtttacaaa ttttttcgta ccattcacct aagttatcac
     2641 tatgaaaagc acgtgaaact aaagtcactt tatcagcttg atcttttaat gcttgaataa
     2701 ttttcggatg tctatgacct tggttaacag cggaatatgc agataacata tccatatatt
     2761 tattgccttc aggatcttta acccataccc cttcagcttc tgaaatgaca attggcaatg
     2821 gtaaataatt atgtgctccg taatgatttg ttaactcaat aattttttca gatttagtca
     2881 tcatatctcc ccttttcatc atttataact attatacatg aaacattatc caaataatta
     2941 cattagtttt caaagcagat acttttccac caaaaaagat gaaataatca ctaagtttca
     3001 ttaaatttgt ctattttgaa aacccttaca tttataatga cataattact taaatgatta
     3061 caagcaaaag aattgataat tttacactta atcaaaagta tattttacta agaatatttt
     3121 tatttataaa tattgaaaac cactaacaaa ttgcatacac aatatcatta gtggtaacag
     3181 ttaaacactt atttatcttt acgtggtaat ggataaaacc cttcattaaa ttgatgccat
     3241 aaattaggag ttaagtgatg attatcttct ctctctggat caaagtaatt aataatttca
     3301 tcttcccgtc catctttaat tttttcaaca aattgataat ccagtagtag ctctctacca
     3361 atggctacta agtcaacacc aacattttca actgcatcta aagcttcgtc agctgtgaaa
     3421 attgaaccaa taccgataag tggcatacga ccatttatcc atttgtgaat taaaggcagt
     3481 ctttcttgtc cagcgtattt accttcacgt gtcgttgcat gcgtatccat cattgaaaca
     3541 tgaatatagt cgattggcat atggctaatt ttattaacga gttcctctgt aatttccatt
     3601 gtgattcctg gagactccgc ttcctctgga gataatctgt atccaactat aaagtcttta
     3661 ttgccatacg cttctttcgc tttaagtact tcttcaatca cagcgaccgg gaatttatat
     3721 tgatttgccc atacatcatt tcttctatta tagtatggtg atacaaattg atgaattaag
     3781 taatgattcg cgccatgtat ttcaacacca tcaaaccctg cttcaattgc acgtcgcgtt
     3841 gcttcaccaa aatccttgat tgcttgttca atctcttcat tcgtcatttc tctagcacta
     3901 tgttcttgtt tctgaccaaa actttttaaa gaaattggac ttggtgctac gacgtctcca
     3961 tcaggtgtta attcaggcaa tgcttgtgca ccgccatgat gtatttgtac gagtgctttg
     4021 gcaccgtttt tcttcattgc tgtagctaat cgttttagtc cttcaatatt actgtcatgc
     4081 gcgattgatg gctgtcctgg aaatgctttt ccgacatcac tcacattact cgcagcatta
     4141 attgtaatac caacatcttg tgaacgcttt tcaatataag gaagttctac atctgaaata
     4201 gtaccatcat catttgaaga aatatgtgtt aaaggggcta acacaaatcg atttctcaac
     4261 tctactccat ttggtaattc tactttatca aacaatggtt cgtatttact tttcattact
     4321 taattcctcc ttaaaattat tgagttgact ataacatgta attttttcat tctccaatta
     4381 tctgtttata tagttgcaat tcgtttaata ctttaagttg tagttctcat ttctaaaata
     4441 aataaaaaaa aacaaatcca catacaatat tgcaaatcaa cgattctatc taacaaagat
     4501 aatggatatg tcaccttaaa ccatttgtac gtgtaaatag aatcaatcat attacgttat
     4561 cgtagacaaa aagagattag tatctaataa aatttaatag atactaatct cttttatagt
     4621 ctaagaaact ttcatttcag accctttgta tgattttcgg tacgatttat ctgttccttt
     4681 agtcgtttcg aattgctcgc tttgactgtt ttatccagat tggtaagcgt tctttaagtg
     4741 tttgaaaccc atatttttct gtttctctga tttcatctaa tactgattgt ttttccttct
     4801 tacgctcata atttttgaag taatcattat cctttaatga tagattaagc tttccttcat
     4861 catctataga caaaatttta gctttaacaa tttggccttc tgatagaaat ttcttcaaat
     4921 tatgaacgta gtcatccata atttctgata tatgaatcag tccttcagta tgattagggg
     4981 tctcaacaaa cgcaccgtat ggttgaatac cagttacacg caccttgata tgttggccaa
     5041 ttttgtagtt attcaactta ataacccctt atttcgttta cttaacacta ttatcatagc
     5101 acaaatcgaa atggtggact aattaaaaca tcaaatcata cgaatattat tttataaatg
     5161 aataagaatt ctacaaactt ggatttacaa ataaaattag caacaaattc ctatctaaat
     5221 caatatgata tccagctact ttgttttcaa tttatttact atttgaatag caattctctg
     5281 taaaaacata aaaagcaacc tcaactatta tctttaaaat acacatctat ttaaaaactg
     5341 tgctttcttg ataaatatat tgagattgct catcatatta gtttatttat tttcgttgga
     5401 atatatacta atccattcat aaattagctt tatccgagtt taaaatgttg gtagttaatt
     5461 atgtttagat attattcttc aacatcaata gattcaatta caacatcatg aagtggttta
     5521 tcttgtggtc ccacttttgt atttgcaata tcttctaatg tagtttcacc atcaatgatt
     5581 tgaccgaata ctgtatgttt ttgatctaac catggtgtac cacccttttc gccatatgca
     5641 tcaacgattg gttgaggcca gccaccatct gcaagttgac ttaacatatt ttgaggtact
     5701 tctttcattt gaacaatgaa aaattgtgaa ccattagtat taggtcctga gttagccatt
     5761 gataatgcgc catataagtt aaatgcattt aatgaaaatt catcttcaaa agcaccgcca
     5821 taaatacttt cgccacccat accagtagct gttggatcgc caccttgaat catgaagtca
     5881 ttaatgacac ggtggaatgt gattccatca taataaccat tttttgcatg tgtcacaaaa
     5941 ttttcaactg tttttggtgc aatatttgga aataatttga atgtcatgtc acctttattt
     6001 gtgtgcataa ccactttgat ttcaccttgt tgtacttctt tgtttaactg tggatagtta
     6061 gccatttata ttctccattc atgttatgat acatatatat taacatactt ttaagggagg
     6121 aaaatagcat gaagctatat cgattttcac acaaaacagg tagctatggt gtcacgattg
     6181 ttgaagaaaa tggagacgat gtccttgtta aagttgaaca agttatcaaa cacccgaaac
     6241 aaggtgattt acataaccct actgaaactg aaggtgtatt tttccatgac cgcaaagcat
     6301 taagccattt tgaaaaacgc tatacgaaac gttcacaatt acgcgacttc aatgttgatg
     6361 tattgcgcta tgaagattca cttcaacaag caattacaga tttagaaact aaacttaaag
     6421 ctcagcaaac aaaacatgct gaaatgtctt tagctagttt agcacgctta aaagaagatt
     6481 attcacttca atacaaagaa aattttaaat aaaaaatgac gtgtttcttc ttatttaaat
     6541 acttctgaaa ataaagcaca acaaggagtg tgtacgtttt tcagaactat atcattcatg
     6601 tataatgaat aacataaata ttttttaaag gaggacaaca tagtgagttt gttacatatt
     6661 gcggtgatat taccgttaat attcgcactc attattccta tcctgtatag attttttaaa
     6721 cgtatacatt taggatggtt cgtattacct gtccctatcg tcatatttat ttatatgctt
     6781 acattaatta aaacgacgat gtcaggtaat actgtaatga aaaccttaaa ttggatgcct
     6841 cattttggta tgaactttga tttatatctt gatggcttag gtttactctt tagtttgctt
     6901 atttcaggaa tcggcagttt agtagtatta tattcaatcg gc
//
LOCUS       DANRDI010000135         6920 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.135, whole genome shotgun sequence.
ACCESSION   DANRDI010000135 DANRDI010000000
VERSION     DANRDI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.135"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..275
                     /gene="yhbY"
                     /locus_tag="PZ377_002189"
     CDS             <1..275
                     /gene="yhbY"
                     /locus_tag="PZ377_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDM6359051.1"
                     /translation="KRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRELIKVHV
                     LQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            278..847
                     /gene="nadD"
                     /locus_tag="PZ377_002190"
     CDS             278..847
                     /gene="nadD"
                     /locus_tag="PZ377_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDM6359052.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            837..1421
                     /gene="yqeK"
                     /locus_tag="PZ377_002191"
     CDS             837..1421
                     /gene="yqeK"
                     /locus_tag="PZ377_002191"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDM6359053.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            1422..1775
                     /gene="rsfS"
                     /locus_tag="PZ377_002192"
     CDS             1422..1775
                     /gene="rsfS"
                     /locus_tag="PZ377_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDM6359054.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            1778..2494
                     /locus_tag="PZ377_002193"
     CDS             1778..2494
                     /locus_tag="PZ377_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDM6359055.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            2534..3220
                     /locus_tag="PZ377_002194"
     CDS             2534..3220
                     /locus_tag="PZ377_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDM6359056.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            3312..3773
                     /locus_tag="PZ377_002195"
     CDS             3312..3773
                     /locus_tag="PZ377_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDM6359057.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            3778..5979
                     /locus_tag="PZ377_002196"
     CDS             3778..5979
                     /locus_tag="PZ377_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDM6359058.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            6036..>6920
                     /gene="holA"
                     /locus_tag="PZ377_002197"
     CDS             6036..>6920
                     /gene="holA"
                     /locus_tag="PZ377_002197"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDM6359059.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNI"
BASE COUNT         2578 a          846 c         1220 g         2276 t
ORIGIN      
        1 aaaaaagata cttaagaagt ttagcacaca atattgatcc gatttttcaa attggaaaag
       61 gcggtatcaa cgaaaatatg attaaacaaa tagatgatac gttagaaaac agagaattga
      121 ttaaagtaca tgtactacaa aataactttg atgataaaaa agaattagct gaaacattaa
      181 gcgaagctac tcatagtgaa ttagtgcaag tgattggatc tatgatagtg atttatagag
      241 aatctaaaga taataaagaa attgaattgc cataataatg aaaaagatag tactttacgg
      301 cggtcagttt aaccctatcc atactgcaca tatgatagta gctagcgaag tatttcatga
      361 attacagcca gatgaatttt attttttacc tagttttatg tctccattga aaaagcacca
      421 tgattttata gacgttcagc acagattaac aatgatacag atgattatcg acgagcttgg
      481 ttttggagat atttgtgacg atgaaattaa acgtggtggt caaagttata cctatgacac
      541 gatcaaggca ttcaaggagc aacacaaaga cagtgagttg tactttgtta ttgggacgga
      601 tcagtataac caactagaga aatggtatca aattgaatac ttaaaagaaa tggttacttt
      661 tgtagttgta aatcgagaca aaaatagtca aaatgttgaa aatgctatga ttgcaattca
      721 gatacctagg gtagatataa gttcgacaat gattcgacaa agagttagtg aagggaaatc
      781 tatccaagtt cttgttccta aatccgttga aaactatatt aagggggaag gattatatga
      841 acattgaaaa agcaaaacgg cttgcaaaag agaaactgcc agagaaacgt tataatcatt
      901 ctttaagagt tgcagaaaca gcgattaaat tggcagaaat ttatgatgga gacactagta
      961 aagtagaatt agcaggtgta ttacatgatt tctgtaaata tgatgattta ggtaaaatgt
     1021 atcaaattgt tcgacaatac gaattaggta atgatctact gagttatggt agtgaaatat
     1081 tgcatggccc tgtgtgtgca gcaatcatgg aacatgaata tggtatcaat gatgaggaag
     1141 tattaatggc tatcaaatac catactactg gacgtcaaca aatgacaaaa actgaaaaac
     1201 tgatttttat tgcagattac atcgaacctg gaagaacaat cccaggagtt gatgatattc
     1261 gagatatggc atacaatcaa ggtagtttag ataagacaat ttatgaaatt tctaaacgca
     1321 cagtactatt tttaatacag aaagatatta cggtatataa taagacgatt gactgtttaa
     1381 attattataa ctatagtgac gaaagaataa aggatgatta aatgaattca caagaattat
     1441 tagcaattgc tgtggatgca attgacaata aaaaaggcga agatacgatt tctttagaaa
     1501 tgaaaggtat cagcgatatg acagattatt ttgttgtaac gcacggaaat aatgaacgac
     1561 aagttcaagc gattgctaga gcggtgaaag aagtagccaa tgaacaaaat atagaagtaa
     1621 aacgtatgga aggatacaat gaagcgcgtt ggatattaat tgacttagct gatgttgtgg
     1681 tacatgtttt ccataaagac gaaagaaatt attataatat tgaaaagtta tatcaagatg
     1741 caccattaga atcatatagt caggttgcgt attaattatg tcgcaatatg cagaaatgag
     1801 cctagtgtac gatcaattga ctcaagatca accatatgaa aaatggtttg aaattgtaaa
     1861 aaatcactgc aaagatgaat caaatatttt agatattgga tgcggtactg gtagtttaac
     1921 agttcaatta gaagctttag gtaatgttac tggtatggat ttaagtgttg atatgttgac
     1981 tgttgcagct caaaaatcag caaatgtaaa ttggcttgaa ggagatatga catcttttga
     2041 tttgcaacaa caatttgata tcatcactat tttttgtgac tctctaaatt atttgcaaga
     2101 tgaaactgca gtgattgaaa cattcatcaa tgtttatcat catctgactg attcaggtgt
     2161 atttattttt gatgtacata ctgtttataa aatgatgact ttgtttaata atcaaagtta
     2221 tattgatgat aaaggggaca tttttttagc atgggatgct gtgcaaggag atttaccttt
     2281 aagtgtttat catgatatga catttttcat tcgacatgaa gatgaaacat attcaagatt
     2341 tgatgagtct cattttcaga gaacatttga tgaaaagaca tatttgtctt ggttagctca
     2401 agttggtttt aaacatgttg aaacatttac tgattttaat atagatgaac ataatgaaga
     2461 tgcagaaaga ttgtttttca ttgcgaaaaa ataaaagtaa aaccactcgc tcagacgtgt
     2521 ataaagttga ggagtggttt tattgtatca atttttatta cgctataaag attttttaac
     2581 tcaatggaag ttatatattc taagtgctgt tgttttaatt atggtattaa ttggttttat
     2641 attctggaga caagatgatt atacttcaag aaattttgaa aataaagata ctgctctgaa
     2701 acaaagcact agtgaaaata gtagtttgtc caaagtagaa gatgtccagg tcaaagatgg
     2761 agataattcc aaaaataagg gtcctgtata tgtcgatgta aaaggtgctg ttaaacatcc
     2821 taatgtttat aaaatgacat ctaaggatag agtagttgat ttacttgata aagcacaatt
     2881 attggaggat gcagatgtaa gtcaaattaa tttgtctgaa aaattaacag atcaaaaaat
     2941 gattttcata cctcataaag gacaaaagaa tgttgaacca caaattgaag taaacagtgt
     3001 gcacgaaaaa aatgggaaca caaataatac taaagtaaat ttaaatacgg catctgtatc
     3061 agaattgatg tctgttcctg gagttgggca agctaaagct aatgcaattg ttgaatatcg
     3121 caaccaacaa ggtgcatttc aagaaattga cgatttgaaa aaagtaaaag gttttggaag
     3181 taaaactttt gataaactga aatcttattt cacgacataa tggattattt taattgcaaa
     3241 ttttattttt tgtgattaat cttatattat tagaaaatgt tactaaattt taagtatgga
     3301 aggtgaacat cttggaaaga atcaaatggg aagaatattt tatggcacaa agtcatttgc
     3361 tagcattacg ttcaacttgt caaagattat ctgtaggtgc aacgattgtt aaggataatc
     3421 gtattattgc tggtggttat aatggctctg tagctggcga ggtgcattgt atagatgaag
     3481 gatgtttaat tgaagatgga cattgtatca gaacgataca tgcagaaatg aatgctttat
     3541 tacaatgtgc aaaacaaggt gtatctactg aaggtgcaac aatctatgtt actcattttc
     3601 catgcctaaa ttgtacaaag tcaattattc aagcaggtat aaagcgtatc tactatgcag
     3661 aagattatca taaccatgaa tatgcaacta aattactcaa acaatctggt attgaattta
     3721 aaaaaattcc attttcacca gaatatgttg ctaaatatct gactaaaggt taatgttttg
     3781 ctgtttgtcg cgttatcaat gattgtagga gtgctttgga attctagcaa agtgctctct
     3841 acatttcttt tcattttact tttgtatatt acttatcgta aaaataaaat cgtttatgcc
     3901 cctatttctc tctttttaat cattttctcc gcatggtatt tacattattc acaacaagca
     3961 atatttaatt atatcaatta tattgaacgt aattctcagt ttaatgagcg tgctcaagta
     4021 atccaaattc aacgtcaagg tagtgacaca tataaaggta ggttgagttt aaaaaatgaa
     4081 atatatcctt tctttttaac agataaaaag aattttgatt taaagaaaat tgaaagtcgt
     4141 aattgtattg ttaaaggaca attcaaagtt aatgacaata agtttgtaac tcttaaatta
     4201 caaagtatag ttgtacaaag ctgcctagaa tcgaaccggt ctaatttaat tgagaaacat
     4261 aaacagttta taatgaatcg aatttatgat tcgggtatta agtttccgga tcgtattatg
     4321 gcattgatta ctggtgacgt aaaagaagtt aatgagcaat ttaaggaacg tgttaaagag
     4381 atagttatat atcatttgct ggcagttagt ggctcgcata tagctgcaat tgtattttta
     4441 atttaccaac ctttaaaacg attaaattta cctttatttg tcattaaagg aattacaatc
     4501 attgtattag ctttatttgc tcaatacaca aattatgcac ctagtgctgt aagagctata
     4561 ataatgacaa ctcttgtact ggttattact aagcaaatta aaataaagag tattcagcta
     4621 ttagcatttg catttataat tatgtttatt ttaaatccac tagttgttta tgatattgga
     4681 tttcaatttt cattcatcat ttcatttttt attatgctac tttttccttt tttacagcaa
     4741 ttgtcaaagt tacaatcatt attcataatt acgtttattg cacaattagc ttcatttatc
     4801 gttgccattc caagctttca tcaacttcaa tgggtgggat ttttatctaa tttgattttt
     4861 gtaccgtact attcgattat attgtttccg ctatctattt tattctttat tacaagtcat
     4921 tttattgtgg gattaacgcc gctaaattac ttggttgacc taagttttaa ttttcatgac
     4981 tggttactag acctattcac aagaatcaag caatcacatt tttctgttcc caagtttaat
     5041 gattggatat ttatagtatt tataatttct gtttattaca tattttggtt attggctaaa
     5101 cgtaaatata tattggttac gttttggact ataattattc tgacattatt aataacgttt
     5161 ccaacaaatt cacatcacaa aattacaatg ttaaatgtgg ggcagggaga cagtatttta
     5221 tatgaaggcg gtaagaacca aaatgtcttg attgatacag gtgggaaagt gattgatgat
     5281 actaaacaac ctagttattc aatttctaaa tatcatattt taccaacgct aaatgaaaga
     5341 gggataaatg aattagagta tctaatttta acacatccac acaatgacca tattggtgaa
     5401 gtggaatata ttattagtca tattaaaatt aaacatatag tgatatacaa taagggatat
     5461 agtagtaata cattgatgtt attatcgaaa ttaagccata agtacaacat taaacttatg
     5521 gatgtaagac aagttagtag ttttaaactt ggagatagta gttttctatt ttttgatagt
     5581 tttattccaa atagccgaga taaaaatgag tattcgatta ttactatgat tacatatcaa
     5641 aataaaaaag ttttattaat gggcgatgct agtaaaaata atgaatcttt actactaaaa
     5701 aaatataact tgccggagat tgatatttta aaagtaggac atcatgggag caagacaagt
     5761 agttctaaag aatttataga gatgattaag cctaaaataa gtttgatttc ttctgggaag
     5821 aacaatatgt atcatcttcc taatatagaa gttgttaaac gattgcaaag gattcgcagt
     5881 cgcatttaca atagtcaaca aaacggtcaa gttacaattg acttagatga taatttaaaa
     5941 gttgattcaa gctcttatgg aaatgcaagt ggtttataga tacagaaaca tgatataatc
     6001 tattgatgtg tcaaacgaat gaaagggtgt aatgaatgag cgacaatatt gtagctattt
     6061 atggagatgt gcctgaattg gttgaaaaac aaagtgcaga aatcatatca caatttttga
     6121 aaagtgatag agatgacttt aactttgtga aatataattt atacgaaaca gagattgcac
     6181 caattgttga agaaacatta acattgcctt tcttttcaga taaaaaagca attttggtta
     6241 aaaatgcata tatatttaca ggtgaaaaag cgccaaaaga tatggctcat aatgtagatc
     6301 aattaataga atttattgaa aaatatgatg gcgaaaattt gattgtcttt gagatatatc
     6361 aaaataaact tgatgaaaga aaaaagttaa ctaaaactct aaaaaagcat gcaaggctta
     6421 aaaaaataga gcaaatgtct gaagaagaaa taaaaaaatg gattcaaagt aaattaaatg
     6481 agaatttcaa agatatcaaa agagatgcat tagatttatt tattgagttg acaggtatta
     6541 actttaatat tgtctcacaa gagatagaaa agttgatttt atttttaggc gatagaccaa
     6601 caattaataa gcaggatgtt aaccaaatta ttaatagaag tttagagcaa aatgtatttt
     6661 tactgactga atacattcag aaaagaaaga aagaacaagc aattcattta gtaaaagatt
     6721 taataactat gaaagaagaa ccaattaaat tacttgcact aattacaagt aattaccgat
     6781 tattttatca atgtaagatt ctgagtcaaa aaggatatag tggacagcaa attgctaaaa
     6841 caataggagt acatccatac agagtaaaat tagcgttagg acaagtaaga cattatcaac
     6901 ttgatgaatt attgaatatt
//
LOCUS       DANRDI010000136         6821 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.136, whole genome shotgun sequence.
ACCESSION   DANRDI010000136 DANRDI010000000
VERSION     DANRDI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.136"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(226..1854)
                     /locus_tag="PZ377_002198"
     CDS             complement(226..1854)
                     /locus_tag="PZ377_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDM6359060.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            complement(1876..3225)
                     /locus_tag="PZ377_002199"
     CDS             complement(1876..3225)
                     /locus_tag="PZ377_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDM6359061.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            complement(3459..5246)
                     /locus_tag="PZ377_002200"
     CDS             complement(3459..5246)
                     /locus_tag="PZ377_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDM6359062.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            complement(5246..5536)
                     /locus_tag="PZ377_002201"
     CDS             complement(5246..5536)
                     /locus_tag="PZ377_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359063.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            5675..6724
                     /locus_tag="PZ377_002202"
     CDS             5675..6724
                     /locus_tag="PZ377_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HDM6359064.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREKTKHNNIE
                     YNVSLFFKALDALKK"
BASE COUNT         2121 a         1052 c         1166 g         2482 t
ORIGIN      
        1 ttcttcgaat ctgcaaatcc caatcatttc ccgataaaaa atcattgtgg gatgttcttt
       61 agcaatttca atataagcat tgtatagtta tgaaaaaatt acgacaataa ctgtttcatt
      121 agataagtgt tattgaaatt gataaagagc aattcttgaa aatagttaga taaaataagc
      181 gaaagaatat agtgaaaatt attgttataa caatgattct attagctaaa tagtaagata
      241 tagtgtttgg ggcaaaaata aagacgaagt gctgagatgc acttcgtcga gttgtttatt
      301 attgaaaagt tgtttaatga tttcgttatt aagtttgagt gtgacataga attgtttttt
      361 atgattacca tcttttttaa tatcaatgcg atcaatcact gatagataca atgctttgag
      421 tcgagatttt tctatgtgct taatatcatg aaagatgtgt tgtaatagtt tactgatttc
      481 tttggcatca aataaagtct tatcttcatt ttgttgattt ttgagttggt tgatttgatt
      541 cgtaatgtca ttgagttgct tttcatattt ttgaatactt ggtctgatta ctgatgttaa
      601 gtccggatta tcctcgatgg ttttaatcaa gttatttatt ttgatttgta cttcatcata
      661 ttgttgttgc ttataagcaa tatcgtgatg aagtgcagcg ccatcaactt gattttcttg
      721 attgacgtgt gttactacgc gttgaatgac tttatcactt ttgactattt caagtatttg
      781 cttcatcaca taatcttcaa tcacatcagc tcttacactg tttgccgaac atactttgga
      841 acccttgttc cgaaaattac tacatgaata gtaacgaata cgtttcttag tcccgtcttt
      901 aagagtattc gtggtattgc ttgctgccat aggtgcgcca cattggggac agtgaataat
      961 gcctgtaagc agattcgttc ctttgccatg gacttggggt ttttgactga cttgtttctt
     1021 acgcatttgt actttatccc ataaatcttg attaataatg ggggaatgct taccttcagc
     1081 tatcactggt ttatcattca gccctttacg acgtttttca ctccaatctt tgtatttcgc
     1141 aaattgaatt ttgccgatat agaaagggtt agctaagatg tatgtgattg aactaatact
     1201 gaaaggtttc ccctttttag tgacatatcc tttgtgattc aatgcattgg caattttacg
     1261 atagccatgt cctttggcat agcactcgaa tatatatttt acaatattag cttcatgttg
     1321 gttaatcatt agctcgtgtt tactatctgg tattttgtca taacctagtg gtaaattgcc
     1381 ttgataatag ccttcttggg cacgtctcgt ttgacccata aatacgttct cgacaatgtt
     1441 attacgttcg aattctgaga aactcgcaag tatctgtaac atgagtttac cagaagaagt
     1501 attgacttcc atacgctctg acaaactgaa aaattcgaca ttttgtttgt gtaaatcttc
     1561 gacaattttg agaagatcag atgtattacg agctaatcgg tttgttttgt ataccataac
     1621 acagtcgata ttgccttctt ttgcatcttt caacatacgt tggagctcag gtctgttcat
     1681 agatttacct gaaataccac ggtcagcgta tatatcttta acttcaaaat gatggaagtc
     1741 acagtattct ttgatttgat tgatttgtcc gtcgatacta taaccttctg tgctttgcat
     1801 ttctgttgat acacgtacat agataccgac acgttttgtt ttaagttgtt gcattatgtt
     1861 acatcctttc ttcatttatg caatcgatga ttgcatggtt tgattgacaa tattgagtgg
     1921 ttcatttttg aaatagattc ctataagatt tttatctttc gtaatgtgaa tggtttcaat
     1981 ataggggtac aatatgttta acgtgaaacg tttttgaata atattttgaa tgatgtgttg
     2041 tatttgatgc ccattgatag atgtagtgcg ttgcggttgt tgacgtaatg attgtgtttg
     2101 ctctctgaac gtttctgcat caatgatgcc ttgtgccaac ttttctatca gttgttcttg
     2161 agtcaatgtg tgatgttttt ctatgtttct ttgtcttttg atgcgtttgt caatcgcact
     2221 tttaattttt gtgtagatgc gttgattttg ataaaagtct cggcacactt ctaatacttt
     2281 atcttcaagt gtttgtgcat tgatgccttt aaaatcacag acaaagcgtg aagcattcat
     2341 gtttttagga cagacgtagt aacgtaatgt atgattcttt tttctaatgg tcatatttgt
     2401 aagtgttgca ttacaacatg ggcatttgat tttttgtttg agttgattat ccgaaggtgt
     2461 ctgtttggtt tgtttttgca atcgaagtct ctgcgcttgc tcatatatac ttgtggaaac
     2521 aatagaagga aacatattgt cgaattggcc atattgattg ttgacacgac cacaataatt
     2581 aggattgatg ataatgttac gaacttgata gggttgtcga ttgatatacg tgttatcttc
     2641 ttctaataac tgtgcaattt tcttataacc atgaccttta atgtaataat tgaatacagc
     2701 ctttaccgtt ggtgactcat tttgattgat gatgaatgtt ccgttgtgat attcgtaacc
     2761 aaaaggtgca tgtgttgtaa tcaatcgacc ttgctttgct ttttcttgaa gcccatttct
     2821 gacttgttct ccaatgttat ccgattcaag ttcggctaaa ctgatgaaga tattaagctt
     2881 gagtcggtcg aaagcttgat ccatatcaaa gtaaccatca tgtacgctta agatatgaac
     2941 atggtacgtt tgacataatt tgatgagttt taatgcattt ttcagattac gatgcaacct
     3001 attaagacga taacagcata atatgtcaca ctgtccttgt tgaattaatt gtgttatttg
     3061 tcgataccca ctacgattat ctttgcgacc tgattgtttg tcgctataaa agttgatatg
     3121 ttgaatatga tgtttttcgg ctattgcttc gatagcttgt ttctgtgctg caagagattg
     3181 ttgtttcatc gtactttgac gtaaatagcc tatgacttgt ttcatatcgg ctcctccttt
     3241 cacagtgata atatatattt atggatgaat tgatatataa gcccaacatc aatgagatgt
     3301 tgggcgtcca tattagtcat ttgtttgatt gatttcttca attaccaaat cggctaatat
     3361 ctcgataagt tcatccatgt ttttcactcc gttatttgtt ctatcttcaa tacgtcgatt
     3421 attcagtttg atgcttcaca gttgtatgat aaagacaatt agaaatcttc gtgaactcct
     3481 gaagggccta tcccttcatt agcggattta aaaagttctt tcgcagcttt gttatcattt
     3541 gacggtgtcc aattttgaag taacgactta tctttagtta atccgaggat agatgcaaac
     3601 tctacatcta attttagatg gtaaaataca agtgattgtt ttttaccgct attatctttg
     3661 acacttcttt tagttgtttg gcgtccacgg tcagctaata tgaaaccttt atctcttaag
     3721 gcgttgacaa cattattaac atcttgaaat tgatgattgt ttagcatctg tttaaaaacg
     3781 ttcgcaatta tttttacttc gatatggtca tcttttaatg agattaatcc atagttctca
     3841 aacatatttt tcaaagcacc ttcatctgaa aacttacctc tgttttgtgc cacaaattga
     3901 atgatgacat caatagcttt atcagctaat gagcgttcag agactgtatg agtatgataa
     3961 tcaataaagt agtctcttat attagcgata tcaatatctg tagataaaac acgacctaat
     4021 attttcgcag atgttgtaat gactgcataa cgcttaaaca tacgaatacc tgtattgttt
     4081 gtttcatctt tcaatttagc ttcaaaccaa tctacttcct tgtaaaacca ttgaataact
     4141 tcatcttcac gatttataag atatttagct actaacggta aaacatgacc atagtttagt
     4201 gctacagctt ttttaatatt gtcagcattg gtcgcatttg tagtgaattg ttcattaatc
     4261 tcgatggttc ttacacgtaa tccatcgttt tgagctgaat cattaaaaat actgtattct
     4321 gacgttgaaa tgacagaagt accccaattc ttaggcgttt taacttctcc atgaacgttt
     4381 gaacgttgac gaccttgacc ttcagcgatg gaatataaca aacccgtggt atctctaagt
     4441 gtcgtagatg aaagctcatc aaatactata ggaatgccat aattgttact caaataacct
     4501 tcaagtgcgt ttcgtgtggc attccaactt ctaaaaagag tttcattacc tttggtaggg
     4561 ttaccagcga ctgatacagc taaagcagct gcggttgact taccggttga ggattgacct
     4621 gtaaaactaa agagaattcc tgcaaattcg atttcatgtt tatgcttcag aaaacttgtc
     4681 actaaggcag aaatcccaaa tatgactgcc aattctaaaa gaagatgacc tttaacctcg
     4741 tcaatataca tgttaaacca attatcaaat gtacctttag gagttaagtc ataagcacta
     4801 tctacaatag ggtatgaagg tgaggactga ttaaattgaa tagatttgag aactttatct
     4861 agcgatatca agtagccaaa aggtgtctct agtataccta caccctcata taattcagaa
     4921 agtggtaatc tatctcgcat caattgcaag gcataactca atgatctaat atacttctcg
     4981 ttaatactgt agccatattt aattaaagaa ggtaagtttc gtgttgttaa gatatcagac
     5041 tcaaaaatgt cttttttacc attgttgtta gtaataatca acttttcaac accagtaatt
     5101 ggatcagaaa atcttgcatt aacaataata gggctagcca ttctaaccac tttttcactg
     5161 tcaccatctt ttttaggtgg tatcgtttct ttccaaccga aggaatctaa agcaaatggg
     5221 ccaatttcaa acaatgtgta actcattagc aatcacctcc tttcgaaggc gtgctattat
     5281 tgcatgggtt tatccctatt ttctgatata ataaactgcc attttttgtt cctacaattt
     5341 taaatggtaa tcctggcgtg tacttaatcc agtaagcaaa acgacgtcct acttcacgtt
     5401 gatcattcgt gctacaaacc acattatatt tctttcttaa ttcaccataa gtgaacgtac
     5461 ttccaactgg ttgttgaaaa gatttagcaa ttataaaatt ataaagcttt tcttgctgct
     5521 tagatttctt attcatttta aaattcctcc aataattttt aaggagtcaa tcaatatcat
     5581 ataggttaaa ctatcttcaa ttgattgcta taataagcat aaatgaagca ttatgattag
     5641 acgcggaaca aaaaatgggg agggggggta tatattgata cctacgctag aagaaattat
     5701 tgataagtat ggaaacttag ttgattattt gaaatttgat gtaacagttg aggtttatga
     5761 agatttgttg ttattaagaa gtttaagcga tttaaatgaa catcaaaaga tggatagagt
     5821 ttcatttata aaaaaacatt taaactcaaa tttcgattat ttattagatg atactgagtt
     5881 tagaagtatc aataagattt acagtaattt aaatatcatg acgcatatca atagtcaaaa
     5941 taataatttt aaacgtaatc catttataaa tgaagaacaa ttgaaatcac tactcgcgat
     6001 aaagaaagta gatgcacata tgagttatga ttcaaatatt acttcaaaag cattggcaga
     6061 aataaacaaa acgcaaaaaa gattagttac aaagatagac acactctata atcagtcaaa
     6121 aaaagaaaat gaatatgtac ggttaggaga aaaaatatcg tatacaaaat tggagaatca
     6181 ttggaaatat ttatataatg agattcagtt ttataatctt aataatcaat tgattagtta
     6241 tgttgcatta gaacaggaat atgcttgggc ttttttaaat gaactatttt atttgataga
     6301 attgtatttg aaggcattta aaggacaaaa gaaaacagat tatgagtttg ggaaaaaatt
     6361 aaacgaattt attcaatcat atgtcattat tttacttata gatatacgat tacctgttgt
     6421 tagattatat attattagag acattgtaga tacatgtgaa ggagaaaaag ataattacaa
     6481 aagaattaca ctgatagaag aatctattaa aaagtataga tacgtaaaag atcagattaa
     6541 aagatttaaa aatggattag aagaatcgtt attaaatgca actttatcta ttgaacaaaa
     6601 tatgttgaaa gttaaaattg attattataa agcctactgc tttcctagag aaaaaacaaa
     6661 gcataacaat attgagtaca atgtatcgtt gttttttaaa gcattagatg ccttaaagaa
     6721 atagtttttc actgaaaata tttatagata taattaaaat taattaatat taagccagga
     6781 taatgtagtc ttaatcgttc tgaaatacga aaaatgttgt g
//
LOCUS       DANRDI010000137         6809 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.137, whole genome shotgun sequence.
ACCESSION   DANRDI010000137 DANRDI010000000
VERSION     DANRDI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.137"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..218
                     /locus_tag="PZ377_002203"
     CDS             <1..218
                     /locus_tag="PZ377_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDM6359065.1"
                     /translation="EDLEPWTSCEFDFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMY
                     KKFGILPDMEYEMEEIRAVEKYVKEQE"
     gene            841..1215
                     /locus_tag="PZ377_002204"
     CDS             841..1215
                     /locus_tag="PZ377_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDM6359066.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            1368..1766
                     /locus_tag="PZ377_002205"
     CDS             1368..1766
                     /locus_tag="PZ377_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDM6359067.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1982..2806)
                     /locus_tag="PZ377_002206"
     CDS             complement(1982..2806)
                     /locus_tag="PZ377_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDM6359068.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(3043..4350)
                     /gene="brnQ"
                     /locus_tag="PZ377_002207"
     CDS             complement(3043..4350)
                     /gene="brnQ"
                     /locus_tag="PZ377_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDM6359069.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4934..5824
                     /locus_tag="PZ377_002208"
     CDS             4934..5824
                     /locus_tag="PZ377_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDM6359070.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            6073..>6809
                     /locus_tag="PZ377_002209"
     CDS             6073..>6809
                     /locus_tag="PZ377_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6359071.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATV"
BASE COUNT         2634 a          950 c         1153 g         2072 t
ORIGIN      
        1 aagaagattt agaaccgtgg acatcatgtg aatttgactt tacaagaaag ggaaatttaa
       61 aagtatcatt tgattatata gattggatta aattaggttt tggcccatca ggaaaggaaa
      121 actactatat gtacaaaaaa tttggtattt taccagatat ggaatatgaa atggaagaaa
      181 ttcgagcagt agagaagtat gttaaagagc aagagtagca gacatgttat aaaagactgt
      241 gcaaaatcac cctcgtttta catttgattc aaagaagaag gtaaaagata agattatttg
      301 caacttaaaa agtcaattag cttatcggta tccatacatc atggataaat gagttcaact
      361 aattaacaaa tcacgatata aatttaaaat tgtcattaat tgaaaaggtt attgtagtgt
      421 attttataaa gttgatgtac atcgacttat tttttaaaac ttatatcaaa aaaatatgag
      481 aaaagtatat actattaaaa tgttttttac tataattata aatagttaaa tatttgggaa
      541 ttaaataaat atataggagg caatttttag agatacatag caatgtaaag gtatcaaata
      601 gttatttcat aatttattat gatgacataa ttttcagtag atagttgtaa attaacactg
      661 aaatagatgt ttatattaag aagactttaa tatcataatt aatcaaaatc ttgcgtcaaa
      721 catttaaacg aattttaagt tgataacaat tggcaaaaca ttgttcaaac atcacaatga
      781 taaagcatat tatcagtatt gtagtgtgtg gaaaatgaca gccatctaag gagaaaaatg
      841 atgaaaagaa tattggtagt atttttaatg ttagcaatta tattggcagg ttgttctaat
      901 aaaggtgaaa agtatcaaaa agatattgat aaagtgtaca aagaacagaa tcaaatgaat
      961 aaaattgcct cgaaagtaca aaacactatt aaaacagaca ttaaacaaga agacagtaat
     1021 acacatgttt ataaagatgg taaagtcatt gttattggta ttcaattata taaagatcgt
     1081 gaaaaaatgt attatttcgc atatgaaata aaagatggta aggcagagat taatagagaa
     1141 atagacccaa ttaagtatat gaaagaccat aaagcagatt atgaagatga aaatgtagaa
     1201 gtggaaaaag attaacatag gctttcttta aaacttttaa ccagtttcaa aaagtaaatg
     1261 agctttagcg acaataattt ttaaaataaa cacgatataa atttaaacat ttatatataa
     1321 gaacacaaac gcaagtttgt aaataataat aataaaggag taagacaatg gaaaaatcga
     1381 tcaaaataat gacaataata ggaattgttg ttcagggttt agcaacggta tttagtttac
     1441 tattgatggt tttagcagca tcaggtgtaa tgactacaga tgtgtcaaca acagttaatg
     1501 gtgaggttga cccagttgat gcagaaacag cagcagcaat tttcactgta ttattcttat
     1561 ccctattcat atttggaatc atttcaatta ttttaggtgc aatcggtatg tttaaagcat
     1621 ctaaaaacaa aaaaatgagt ggtatattgt tgattattgg agctgtaata agtggtaaca
     1681 taattacatt tgctttatgg ttaatcagtg gtattaaact acttactaat aacaagccta
     1741 aagatgaaat aagcgactta tcataaacat cgtatattga aatttcaaga tttcttaagt
     1801 aattaaataa agtgctctct tagaagtaga ttttcagtca ataactgctt ttaagagagc
     1861 attttgtatt tgataatgct catagcatcg gataaaaatc attgacgaac tttaaaaaaa
     1921 tacgttagac aatgcacttc taaatattaa aggcatcgac taacgcattg ttcaaatata
     1981 gttaattatt tttataaaat tgatgatgat cattcaagta agcatagaat aaacctataa
     2041 tgagtccgcc tccaatatag ttaccgataa aagccgcagc gatattcgaa atagctggta
     2101 tgaagtgcaa tgtatcaact tgataaatta aaccacccat aaataagcaa ctgttgtaaa
     2161 cgacgtgttc ataacccata aaggcgaata tggtaacacc gaacatcatg acaaacattt
     2221 ttgcgagtac atcgtcaatt tgcatggcaa taactaatga aatattgata aagaaattgg
     2281 cgaagatcgc tttcattaat atacttacaa aaccagtaga caacgtttta tgctctataa
     2341 ctgctgataa ctgatttaac atatctggcg tcattacatt tgaaaaacgc atgaaactaa
     2401 ataaaatagc agcacctaaa atatttcctg caaagcataa taaaaatatt ttcaatactc
     2461 tagttggttt aattacttta taatacaggc ctacagtaaa gtacatgaag ttactggtta
     2521 gtagttcgga gtttgtaaat aaaatgagta ctaacgcaaa gctgaatgta atggcactgg
     2581 ccatattcac aatgcctggc ggtaaatctg gttcgtgtgt tgctttaact gataatacga
     2641 agaccgtaat aatcccgata ataaatcctg ccatcatcgc gcgtaataaa taacgtttta
     2701 aataaacgct ttgtaatata tcttttgttc ttatcgtttc gactacgtta tttacccagt
     2761 cgtccccata aaaaatctta tcccatttaa tatgtttctc cttcacaatg acatcctcac
     2821 ttttcatttg ttaatcagag tatactatga attgccttat ttgtgaataa tttcacaaaa
     2881 ttaattttaa aaactaatat ttttgaataa cagctaggat tttttaacaa atcatttatt
     2941 ttaaaataat caatagacag acggtatcac tgaaaagtca tgaatttcat ataaaaaagc
     3001 ctagtaacga tgggtcaatc attactaggc agtgagcatt tattaagttg tcgcttgttt
     3061 cggacggcgt ataaatacat cgattatgaa accgataata gcaaagagca tgaatggtac
     3121 aagccaagct aaatcgatat ctgctaaagg taacatcata aacgatttca aaataacacc
     3181 gtgtaataag ttgaaactat ttagtatttg taaaattgaa ataatcaatg taataacagt
     3241 tgcgagtcga taggcccaac tgaatctgaa tgtgctaaac atgttagcaa atgatatgag
     3301 tacaagtgca atcgacacgg gatatattaa agtcaataat gggacagcaa tttttaaaat
     3361 catttctaaa ccaagtgttg taaataagaa ccctatgata gagaaaataa gtgcgaatat
     3421 tttataagaa aacttaggta cgtgtttctt agtaaatgtg gcgcaagcat tgacgagtcc
     3481 tatacatgtt gttaggcatg caaggataac cgtcattcca aatacgaggt taccgaacga
     3541 accaaataat cgtaatgagt tgtacgtcaa tatatctgta ccatctttaa agtttcctgg
     3601 agctgttgat gccccaacgt atgcaagtgc aaagtaaatc attccaagta atatggctgc
     3661 aataagacct gaaaagcaga catattttaa aattttcatg cgatctgtga ggcctttaaa
     3721 cttatagcca ttgacaatga ctacggaaaa agctaacgca gcaacaagat ccattgtaaa
     3781 atagccttcc aaacttcctg aaatgaaagg atgtgttata tatttatcct taggtgcact
     3841 tagtgcagat tcagggttga aaatgacagc aatacttaat agagcgacca ttaatagtaa
     3901 taacggtgtt aataatttac ctaaattatc aacgattttc gatggattta aactaatcca
     3961 gtaaacgatg gcaaaaaaga ttgctgcgaa tataattaaa gtccattggt tatgcacagg
     4021 taaaatgtgt cttgtaccaa tttcgtacgc gacatttgca gcacgtggaa taccgtaaaa
     4081 tgctccgata gacatgtaaa tcacgacagc aaaaataaac ccgaaccatg gatgtatacg
     4141 attgcctaca ctttcaacac cttcatcata aaatgcaaca acaataacag taataaaggg
     4201 gagtaatatg cctgtaaggg caaagcctag cataccaatc cacatatttt gacccgctgt
     4261 atggccaagc atgggcggga atattaaatt tccggctcca aaaaatagtg aaaataacat
     4321 gaggcccgaa ataataactt gttttttcaa tgtaatttcc ttcctaaaac tagatattca
     4381 taataattta aaaaatctga aaaataaaaa cgttcttact ttatctttaa atgacaatac
     4441 tatgattcta ttattttata aaaaagattg caataataac gtcttgctat tattggataa
     4501 tgacaattaa agtgtcgttt ttatgaatta aaataggtga aagtaagttg gaaatacgtt
     4561 ggagcgttgt gatttttatg agaaattgta tgagtgagga ctgctattta taaaaggtaa
     4621 actatggatg agtttatggt tgttccaata ggtaaataag agatagcaca ctgtacacat
     4681 aatgatacgt ggatgagtga atatgtctga taggaaggcc tagtcactca aatgaattta
     4741 agtttattac gtgataaatc acaaatctct ctcatgtgat aggtctccca ttaaatcatg
     4801 cttatatgaa atgttcacat atttgttagc ttttcaagaa ataatattaa atatcttttc
     4861 atagtatagc aatattaaat aaatgcattt ataattttaa ctgtatttta aatattaatc
     4921 atgaggtgat aagatgaata aaatttcaaa gtatattgca atagcatcat tatcggtagc
     4981 ggttacagtt tcagcaccac aaacgacaaa ttctacagcg tttgccaaaa gttctgctga
     5041 agttcaacaa acgcaacaag cttctatacc agcatcacaa aaggcgaatc ttggtaatca
     5101 aaatattatg gcagtggctt ggtatcaaaa ttcagctgaa gcaaaagcat tatatttaca
     5161 aggttataac agtgcaaaga cacagttaga taaagagatt aaaaagaata aaggtaaaca
     5221 taagttagct attgctttgg atttagatga aacagtttta gataattctc catatcaagg
     5281 ctatgcatca atacataata aacctttccc agaaggttgg catgaatggg tacaagctgc
     5341 taaagctaaa cctgtctatg gcgcaaaaga attcttgaaa tatgctgaca aaaaaggtgt
     5401 cgatatctac tatatttctg atagagataa agaaaaagat ttaaaggcaa cacaaaagaa
     5461 cttaaaacaa caaggtatcc ctcaagctaa gaagagtcat attttactaa aaggtaaaga
     5521 tgataagagt aaagaatcac gcagacaaat ggttcaaaag gatcataaac ttgtcatgct
     5581 atttggagat aatttattag actttacaga tccaaaagaa gctacagctg aatctcgtga
     5641 agcattaatt gaaaaacata aagacgattt cggtaagaaa tatatcattt tccctaaccc
     5701 aatgtatggt agttgggaag ctacgattta caacaataac tataaagcaa gtgacaaagc
     5761 aaaagataaa ttacgtaaaa atgctattaa gcaattcgat cctaaaacag gcgaagttaa
     5821 ataatatatg aattggacgt ctacatgtac ttaaaggtat atgtaggcgt ttttaaattg
     5881 atccttaatt ttaaatgtac cgtacaaaaa taatagacaa ttaattgcat tttccataaa
     5941 tttgctttta atataaaaag ttcgggtata atgaaggata agtgtatgta taacattaca
     6001 gagcgcaaca cgacgtacgt gcatctaaga atataaacaa tgtttaaatc atagtagtag
     6061 ggagagaaat gcatgttttt agcttggaat gaaatacggc gcaacaaatt gaagtttgga
     6121 ctaattattg gtgtgttaac gatgattagt tacttgctat ttttattatc tggattggcg
     6181 aatggtctta tcaatatgaa taaagaaggc attgataagt ggcaagcaga tgccattgtt
     6241 ctaaataaag atgccaatca aactgtgcaa caatctgttt ttaacaagaa agatattgaa
     6301 aataaataca agaagcaagc tactttgaag caaacagggg aaattgtgtc taatggccat
     6361 caaaaagaca atgttttagt gttcggtgtt gaaaagtcat catttttagt tccgagttta
     6421 atagaagggc ataaagcgac taaagataat gaagtgttag ctgatgaaac gcttaaaaat
     6481 aaaggattta aaattggtga cacattatca ctatctcaat cagatgaaaa attgcatatc
     6541 gtaggtttta cagaaagtgc aaaatataat gcgtcatcag tcattttcac gaatgacgct
     6601 accattgcca agatcaatcc tagattgact ggagataaaa ttaatgcagt tgttgtacgt
     6661 gatacaaatt ggaaagacaa aaaattaaac caagagcttg aagcggtaag tattaatgac
     6721 tttattgaaa atttaccagg ttataaacca cagaacttaa cattaaactt tatgatttca
     6781 ttcttatttg tcatttcagc tacagttat
//
LOCUS       DANRDI010000138         6631 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.139, whole genome shotgun sequence.
ACCESSION   DANRDI010000138 DANRDI010000000
VERSION     DANRDI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.139"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(40..732)
                     /locus_tag="PZ377_002210"
     CDS             complement(40..732)
                     /locus_tag="PZ377_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDM6359072.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1117..1914
                     /locus_tag="PZ377_002211"
     CDS             1117..1914
                     /locus_tag="PZ377_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDM6359073.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2047..2994
                     /locus_tag="PZ377_002212"
     CDS             2047..2994
                     /locus_tag="PZ377_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDM6359074.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            3596..6550
                     /gene="fdhF"
                     /locus_tag="PZ377_002213"
     CDS             3596..6550
                     /gene="fdhF"
                     /locus_tag="PZ377_002213"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDM6359075.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            6550..>6631
                     /locus_tag="PZ377_002214"
     CDS             6550..>6631
                     /locus_tag="PZ377_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDM6359076.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEV"
BASE COUNT         2369 a          886 c         1296 g         2080 t
ORIGIN      
        1 aagatatttt ctaattgaaa atcatcttac tgctgttttt taggaatcga tggggtttaa
       61 aaattgatga tagttatcaa tggatatgtc tctggtttga atcgtcttac cgaatgaata
      121 cgaaatatct ataatatttt caatttcgag ttcggcagtt ttgtcttgag caatgtcata
      181 aatgactgca tgccaatgtc catgtttaca caaaaggcca ataaatataa cgttttcagc
      241 tttaatatta tcatcaaccc tatgatctac taacatcaca tttcgttctg tacaataaat
      301 taaaatgtcg gaaaacagca ttgggagtgt catatgttgt gtgtcatcaa atttaatata
      361 aaagtccatg cgtttaagca attttcttaa gcgtgtttgt ggaagactta aacattgttt
      421 tataattgcg ttgatttcgc ttttatatgg taatgtcgag tacgactgac tttcattaag
      481 cactaaaaat aaggctgata attgtgtttc agataagttt aatgcaattt tagattgtgc
      541 ttgttgtatt tggtaaccac catttttccc ataatgcgca taaattttaa caccttgatt
      601 ttctaaatca tcaatatctc ttaaaattgt gcgtttggat acgttgcaat atttagctaa
      661 ttctaaagcg gtcatttttt tattttgttg aattgcagta attattaaat tttgcctttc
      721 agctttattc atgacagcca cctcctaaaa gatgatatat tacacaaagt ataacataag
      781 agtgacaatg aggacatttt caatatcttt taaaaaaaat cgtaaaaatt ttgtggaaaa
      841 cgcttacatt ttaaaaaaga ttgtgctatg atattacttg taacaggggg agcgattaaa
      901 caaaggggta gagctatata acagatagct tttaatcgtt caagttacat cacaacatct
      961 tcaatattta ttacttactt tcctttctat ttgtcggcta gcacgtgact agccgatttt
     1021 tttatgcata aaatgaagaa gtcttcagtt gtattgttga ttcttttaat atagggtaat
     1081 aataaaatga taatgatatg agaagtggtg gacgaaatga cagataaaac attacagcaa
     1141 atagataagt taatatgttc gtggttgaaa caaatagaca atgttattcc gcaattaatt
     1201 atggaaatga ctacagaaac taagcgtcat cgatttgatt tagttacaaa tgtagataaa
     1261 cagattcaac aacaattcca acaattttta gcaacacatt ttccggaaca ccaactatta
     1321 gcggaagaaa aaagtaatga aatgattacg aatgaaatta atcatttatg gattatggat
     1381 cccattgatg gaactgccaa tttagtaaaa cagcaagaag attattgtat tatattggcg
     1441 tatttttatg aaggtaaacc aatgttatcg tacgtatatg attatccgca taaaaagctt
     1501 tataaagcaa tacgaggaga aggtgctttt tgtaatggaa ttaagatgga agaaccacca
     1561 tcgttaaaat tggaagacgc tattatatcg tttaatgcac aagtgatgaa tctagatacg
     1621 gtgcaagatt tatttgacgc ctcatttagt tatcgtttag ttggtgcttg cggattagat
     1681 tctatgcgag tggcaaaagg tcaatttggg gcacatatta atacaaatcc taaaccttgg
     1741 gacattgctg cacagttttt atttgcagaa ctattgaatc taaaaatgac gactttagat
     1801 ggtaaggcaa ttgatcattt aaagggtgca ccttttatta taagtaataa agcatgtcat
     1861 gaaacggtac ttaaaatttt aaatgcaaat ggtggttatc aaaaatacag atagatattc
     1921 tctagagata aaaaatgata aaaaatagtt atacgtctac agaagtatag taaaaaaatt
     1981 attttactat ataataaata gagaaaacag acaaagtatg taaggaatag taaaggagtg
     2041 gtgggattga aacgttcgtc taaaagtaaa atgagcctac cgaaaaaaat atttttatgg
     2101 gtattcggta ttttagtaat cttagcgatt gtagctgttg tgtatgtagc tgctaaaatt
     2161 tttattactg gtaataagat acacaatccg ttagatcgca atcattcaga attaagagat
     2221 aaaaaagtga gtttaaacga tggtgatcca ttcacaatcg ctttatttgg tgtcgattca
     2281 gatgctgatc gtaagaaaaa gggtggcgga gagcgtagtg atagtattat gattttatct
     2341 atcaacccta aaacgaagaa aactgaaata gtaagtatac cgcgtgatac aagagcggaa
     2401 attgttggcc gtggtactac tgaaaaaatt gcacatgcct atgcatatgg tggaccaaat
     2461 atggctgtta aatcacttga aaaattaatg aatgtgccaa ttgatcatta tgcgacaatc
     2521 gatatggatg gtttgcataa tatgatagat agcatcggcg gtgttgatgt agtaagtaat
     2581 gatacattca ctgttgatgg tgttcgtttt acaaaaggac agcagacaca tgtaaatggt
     2641 gatcaagcat tgaagtttat tagaagtcgt aaagaagaag gtgctggtgg tgactttggt
     2701 cgacaacaac gtcagcaaat tgtgcttgaa gcgatggcca ataagattgc aagcccatca
     2761 tcgattacac attttaatag tctaatgaat gaaattcaaa ataatgttaa aacagattta
     2821 acattaggtg atttgaatac gattagaagc aattataaag atgctaatga cacgattaat
     2881 aaacatcaat tgagtggtca aggcggcatt caaagtgacg gattgtacta cttcattcct
     2941 agtgagcagt caaaagctga aagtactaag ttattaaaag ataatctaga gtagtaaact
     3001 aagggaaaat aagttatcaa aattgtacac aaattaatta gatagaatga tctgtaaata
     3061 gaataacgaa tagaactaat agcccgtaaa tagaaggtga taacactttg tatttatggg
     3121 ctattttatt taatgcctga tttgattttt ataaattaat ccattacaaa tttaatttaa
     3181 agataaatat taccttctaa atgatagtag atatttatca aacttgggtt attaattttc
     3241 agaaaaaggg tatttataaa tagtataacg atttttaggt gaggtgatta ttaaatattg
     3301 tcattaataa ggttgtgaaa gatatggcaa aaagcgtgaa acagataatt ggaaaactaa
     3361 taatgataat tattttcaat taaacaaaaa gatgatttaa tttcgcgcaa aatatttcaa
     3421 ataaaaatga ttgtaaaaag gcaaaataca aaattttact taacaactag tacataaagt
     3481 aatacaatta aattaattct atctgaaaga tgtgtggggc atcgttattt taggtggata
     3541 tgagcaattt attaaaagtc atttacggaa aatatatata gacggggtga gtaatatgca
     3601 agaacatttg gtggttacac ttgatggaaa agattatctt gtagaaccag gtacgaattt
     3661 acttgaattt attaaatcac aagatacttt tgtaccttca atttgttata acgagtcgat
     3721 gggaccaatt caaacatgtg atacatgtac tgttgagatt gacggtaaaa ttgaacgttc
     3781 atgtagtacg gtgattgatc gtccaatgac tgtaaatact gtgaacaatg atgtgaaaga
     3841 tgctcaaaaa gaagcgcttg atcgaatttt agaaaagcat atgctgtatt gtacagtatg
     3901 tgattataat aatggtgatt gtgaaattca taatacgatg gatgcatggg ggcttcagca
     3961 tcaaacgtat gaatataaag agaagccata cgaaaaagat tacggcccat tttatcgtta
     4021 tgatccaaat caatgtatct tatgtgggcg ttgtgtagaa gcatgtcaag atatcgaagt
     4081 gaatgaaact attagaattg attgggatcg tgaacatcca cgtgttattt gggataatga
     4141 tgtaccgatt aatgagtctt catgtgtatc ttgtggtcaa tgtgcgacgg tatgtccatg
     4201 taatgccatg atggaagtga atatggaagg taatgcgggt tatatgactg atacggaacc
     4261 tggttcatta gcagcaatga ttgatttaac taaaaaagca gaacctggtt atggtccact
     4321 atttgcaatc tcagattcag aagcagaaat gcgtaaagag cgtattaaga aaactaaaac
     4381 agtgtgtact tattgtggtg tgggttgttc atttgaagtt tggactaaag atagagaaat
     4441 tttaaaagta caaccatcac atgattctcc agcaaataaa attgcgactt gtgttaaagg
     4501 taagttttca tggggacata ttaattcaga tcaacgatta actaaaccac tagtaaggaa
     4561 aaatggtgag ttccatgaag tagaatggga tgaagcttta aacgtcattg cagataattt
     4621 tacagctatt aaagaaaagc atggcccaga tgcactatca ttcatttctt cttctaaagc
     4681 gacgaatgaa gaatcgtatt taatgcaaaa attagcaaga caagttattg gcacaaataa
     4741 cgttgataac tgttcaagat attgccaagc acctgcaaca aaaggcttat ttagaacggt
     4801 tggacacggc ggtgactcag gtagtattga agatttagaa aaagcggcaa tgtctgtatt
     4861 gataggtact aatacagccg aagctcatcc agttatcgca tcacgcatga aacgtgcaca
     4921 aaaattattt ggtcaaaaaa tacatgtatt tgatattaga aaacatgaaa tggcagaacg
     4981 tgcggatcgt ttttatcaac ctaaaccagg tacggattta gcgtggttaa gtgcagtgac
     5041 taagtatatt attgatcatg atttacacga taaagcattt attgatgagt gggtagatga
     5101 ttttgatgaa tattacaaat cattagaaac atttacaatg gcttttgctg aagaagcaac
     5161 aggtattcct gaatcagaat tgattaaatt tgctgaagaa tgtgctaaag ctgaatctgt
     5221 tgtaatttgt tgggcaatgg gtattacaca acaagacatt ggtagtgact caagtacagc
     5281 gatttcaaac ttattattag taacaggtaa ttatcgtcgt cctggtactg gtgcgtatcc
     5341 attacgtgga cataataatg ttcaaggatg tagtgatatg ggaagtatgc ctgataagat
     5401 tactggttat caaagtattg aagcggatga tattcgcgct aaatttgaaa aagaatatgg
     5461 cgttaaattg aatccaaaag ctggtaaaga taatcatgaa atggtagaag gtatacatga
     5521 cggagaagta cactcattgt acttatatgg tgaagatacg ggtattgtgg attcaaatat
     5581 taattttgta caagctgcgt ttgaaaaatt agatttcatg gtagtccaag atgaattttt
     5641 aacattcaca gcaacatacg cagatgttgt attgccagca agtccttcac ttgaaaaaga
     5701 cggtactttt acaaataccg aacgtcgtat tcaacgttta taccaagcat tagaacctct
     5761 tggtgattca aaacctgact ggaaaatttt ccaagcaatt gctaatagat taggatttga
     5821 ttggaattac aagcatccta gtgaaattat ggatgaagta gcacgcttaa cacctctata
     5881 tgctggggta agttatgatc gtttagaagg attcaatagt ttacaatggc cagtacaacc
     5941 tgatggtact gatgagccta ttctatactt agaaggattc aattttgata atggtaaagc
     6001 aaaattattc ccattatcat ttgataatta ctttaagcaa gatgaaattt atgatattca
     6061 tgtaaacaat ggtcgattgt tagagcactt ccatgaaggt aatatgactt atcaaacacc
     6121 aatgattaaa tacaaagtgc cacgtgcatt tgttgaaatt tctccagagc ttgctgaaga
     6181 tagaggcatt catgaaggtg cagaagttaa gctgatttct gaaacaggag aagcggtatt
     6241 acaagttcac gtgacagatc gtgttaaagg taaagaaatc tatattccat taaataatga
     6301 tgcgatggaa aatggagatc taggtgcaat taacttatta actaatagtg atgttgatca
     6361 atacacggat acaccatctt ataaacgtac tagctgtcgt ttggaggtaa ttacgaaacg
     6421 tggtaagtca ccgttgaacc ctaataattt ccgtgtcaat aaaaaacgtc aaccgcagta
     6481 cagtgttcaa gtacagaaaa aatgggaacg ttcagattat gttttcccgg gaaatcaggt
     6541 ggataaataa tggctgagag aatatcttct aaaattagac gcttagaaaa atcagaagaa
     6601 caaattaagt tagaaagttt aaatgaagtt a
//
LOCUS       DANRDI010000139         6600 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.140, whole genome shotgun sequence.
ACCESSION   DANRDI010000139 DANRDI010000000
VERSION     DANRDI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.140"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            190..1251
                     /locus_tag="PZ377_002215"
     CDS             190..1251
                     /locus_tag="PZ377_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDM6359077.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            1509..2966
                     /locus_tag="PZ377_002216"
     CDS             1509..2966
                     /locus_tag="PZ377_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDM6359078.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            2959..3681
                     /locus_tag="PZ377_002217"
     CDS             2959..3681
                     /locus_tag="PZ377_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDM6359079.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            3832..4959
                     /gene="queG"
                     /locus_tag="PZ377_002218"
     CDS             3832..4959
                     /gene="queG"
                     /locus_tag="PZ377_002218"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDM6359080.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            4964..5434
                     /gene="trmL"
                     /locus_tag="PZ377_002219"
     CDS             4964..5434
                     /gene="trmL"
                     /locus_tag="PZ377_002219"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDM6359081.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            5593..6192
                     /locus_tag="PZ377_002220"
     CDS             5593..6192
                     /locus_tag="PZ377_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDM6359082.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            6217..6369
                     /locus_tag="PZ377_002221"
     CDS             6217..6369
                     /locus_tag="PZ377_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359083.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         2359 a          837 c         1249 g         2155 t
ORIGIN      
        1 cattattagt cacaggatgt aattagtagt attttaaaat taacatgaca ttgattagtg
       61 agtagattgt ttaaatttgc tttattcaat ttgtggattg ttatatttag attaaattag
      121 ccaactaatc attgttaaag ttaattttct ataatatgat gagtttaatg ttcaaattgg
      181 aggtcgttta tgagtaacgc aaataatgct gataataaac agtttttcag taagatttta
      241 aattctattg gtgctggtgt tgttattgca cttgttccga atgctttatt aggtgaagta
      301 ttaaaaatat ttaaaagtgg taatgaaatt ttagaattaa cgtatcagtt agtgatacta
      361 attcaatcat ttatggcatt tattattggg gttttagctg ctcatcaatt taaatttaat
      421 ggtgcaggtg ctgctatagt aggtacatca gctatgattg gttcaggtgc tgttgtatat
      481 agcaataata gttttatgtt aaaaggtata ggtgacatta taaatacgag tttggttgtt
      541 attattgcat gtcttatata tatggtatta caaaacaaac ttggatcttt tgaattaatt
      601 atattaccag ttttagtacc tatcgttagt gggggtatcg gtctaatcac attaccttac
      661 attcgaaaaa ttactcaagc aattggaaat gtaattcatt cttttacaga cttaaatccg
      721 ttgttaatgt ctatacttat tagtgtggca ttttcattat taatggtaac accaatatcg
      781 ttagtagcga ttgcaactgc gattagttta aacggactag gaagtggagc agcaaaccta
      841 ggtatagtgg ctgcctgtgt tactttctta tttggctcat tgcgtgtcaa ttctattggt
      901 gtaaatgcag ttttactcat aggagctgca aaaatgatga tcccagttta cttgaaaaat
      961 ttaattattt cgataccatt gactataaat ggaataatta ctggaatcat tgcttacgtt
     1021 cttcaggtta aaggaacacc attgtctgct ggatttggat atacaggatt agttggccca
     1081 ataaatgcat ttaaccgaat gtcaggtgat ccaacaatga atattatttt attagcactt
     1141 ggttattttg tcataccatt tgtatcggca tttattgttc atgaattgtg taaaaaattc
     1201 attcctatat attctaatga tatttataaa tttgaagttc caaaacaata atttatagct
     1261 tgatgacaag aataagtttt aatatatgat aaatataggc gttactgtca ataagatatt
     1321 gatagtagcg cttttttgat tgattttaaa atgtgagatt tgttcacgta taatagcgga
     1381 ttttttatgt acagtctttt aaaaagaagc ttttctttat attgataaaa atacatttat
     1441 aaaacactat tgcatcaaaa tgtattaata tgtataatta tgaatattaa ttagggaggg
     1501 ctgtataaat gaagtgttta attaggttta tattagtatt agggttgtta attagcagtg
     1561 ctatggtgta tatcaatccc acagcacatg ctgaacaaga tcaaacatgg gagaagatta
     1621 aagaacgcgg tgaacttaga gtgggtcttt ctgcagatta tgcaccaatg gaatttgagc
     1681 atacagttaa tggtaagact gagtatgcag gtgtagatat tgatttagct aaaaaaattg
     1741 cgaaagataa taatttaaaa ttaaaaatcg tcaatatgtc atttgatagt ttgttaggag
     1801 ctcttaaaac tggaaaaatt gatattatta tttccggaat gacttcaacg cctgaacgta
     1861 agaagcaagt tgatttttca gattcatata tgatgactaa aaatatcatg cttgtaaaga
     1921 aagataaagt taatgaatat aaagatatca aagactttaa taataaaaaa gtaggggcac
     1981 aaaagggaac tgaacaagaa aaaatcgctc aaaccgaaat tgaaaatgca tctattactt
     2041 cattaagccg tttaccagac gttatattag cattgaaaag tggaaaggtt gaaggtgctg
     2101 tagttgaaaa acctgtagca gaagcatatt taaaacaaaa tcctaaatta ggaatttcca
     2161 atgtgaaatt taatgaagaa gaaaaagata cagtgatagc agtgccaaaa gattcaccaa
     2221 aattattgtc acaaattaat aaaacgatta aggaggttaa agataaagga ttaatcgata
     2281 aatatatgac taatgctgca aatgcgatga atgatgacag tggttttatt tctaagtatg
     2341 gtagtttttt cttgaaagga attaagatta caatattaat ttcacttatc ggtgttgcat
     2401 taggttctat tttaggtgca ttcgttgcgt taatgaaatt aagtaaaata aaaattattt
     2461 catggattgc ttctatctat atcgaaatat taagaggaac accaatgtta gttcaagtat
     2521 ttatcgtatt ctttggtata actgctgcat taggactaga catttcagct cttgtttgtg
     2581 gaacaattgc attagttatt aattcctcag cttacattgc tgaaattatt cgtgcaggta
     2641 taaatgctgt tgataaaggc caaatggaag ccgcacgtag tctaggtttg aattatagac
     2701 aaacgatgaa aagtgtaatt atgccacaag caattaaaaa tattttacca gctttaggta
     2761 atgaatttgt cactttaatt aaagaatcat ctattgtgtc aacaattgga gttggcgaaa
     2821 ttatgtttaa tgcacaagtg gttcaaggta tttcatttga cccattcaca ccattaatag
     2881 tggcagcagc attatacttt gtcttaacat ttgtacttac acgcattatg aacatgattg
     2941 aagggagatt gaatgccagt gattaaaata aacaatctta ataaagtttt tggagataat
     3001 gaagttttaa aagatatcaa tcttgaaatc aatcaagggg aagtagtagc aataataggt
     3061 ccatctggta gtggtaaaag tacattgtta agatgtatga atttattaga agtacctact
     3121 aaaggtcaag tgatttttga aggcaatgac ttaacggaaa aagggacaca agtagataaa
     3181 ctacgtcaaa aaatgggtat ggtatttcaa aacttcaacc tatttccaca taaaaaagtt
     3241 gtcgataata ttattttagc tcctaaatta ttaaagaaag ataataacga tgaattacat
     3301 aaggaagcat tgtcgttatt agataaagtg ggattaaaag aaaaagcaga tgtatatccg
     3361 aatcaattat caggtggtca aaaacaaagg gtagcaattg caagagcttt agcaatgcat
     3421 ccagatgtta ttttattcga tgaaccaact tcagcattag atcctgaggt agttggtgat
     3481 gtattaaaag taatgaaaga cctagccaaa gaaggtatga ccatggtggt tgtgacacat
     3541 gaaatgggat ttgccaaaga tgtaagtgac aaagtcatat ttatggcaga tggcgttgtc
     3601 gtagagtcag gcacaccagt cgaaatattt gaacaaccgc aacatgaaag aacacaaaat
     3661 ttcttagcaa gagtattata acaacctaac gaagcttgaa tatatgatac gcaccacaaa
     3721 gttatatcat atattcaagc tttttattat taaaattatg tgaaatgaaa cctcaatatg
     3781 aataatggtt gtgttaaaat aacatagtat tattttatgg ggggcatacg attggataca
     3841 aagcaattaa agcaagatat cattgactat gcatatacaa ttggtatcga cagtattgga
     3901 tttactactg ccgatccctt tgatgaattg aagcaaaagc tagaagcata tcatgcaaat
     3961 ggttatgcct caggatttga agaatctgat attgctttac gaacggagcc taaattatcc
     4021 ttaccaacag caaggtcaat catagcaatt gcagttggtt atcctaacaa actgaaaggt
     4081 gcacctaaga gtgttagagg tgatcgcaga ggcttatttg ctagagcatc gtggggtcaa
     4141 gattatcata caattatgcg taaacgatta gacatgttag ctgcatttat tgaatctaaa
     4201 gttccagatg ttgaaatcaa atctatggta gatacgggtg tattatcaga tagggcagta
     4261 gcagaacgtg caggtttagg atttgttggt cgtaatggct ttgtcatcaa tcctaaacta
     4321 ggaacatgga catacctcgg tgaaatgtta gtcagtatac cttttgaacc tgatgatcca
     4381 ttattagata gctgtggtga ttgtacaatt tgtgttgatc gttgtccaac aagtgcatta
     4441 gttggaaatg gccaattaaa tagtcagaaa tgcattagtt ttttaacgca aacaaaaggc
     4501 tatatgcctg accaatatcg ttataaaatt gggaatagat tatatggttg cgatacgtgt
     4561 caacaagttt gtccgaaaaa tagaggtatt aataccgaac aagatgacat cattttggaa
     4621 ccagaaattt taaagccaag attagtacct ttactacgta tgtctaataa agaattcaaa
     4681 caaacatatg gtcaccttgc aggtgcttgg cgtggtaaaa agccgataca acgaaatgct
     4741 attttagcat tggcgcattt taatgaagtg gatgcaattc cagaattgaa aaaagttgca
     4801 acaacagatg aaagaccgtt gattcgagca actgcttatt gggccattgg tcaaattctt
     4861 ggggaagaag caagagattt tatcaatgct aattatgatc aagaggatgc agaagttcag
     4921 aatgaaatga taaaaggatt agacacaagg agagaatagt aatatgacaa atcatatcgt
     4981 tttatatcaa ccagaaatcc cagctaatac tggtaacatt gcacgtacgt gtgcaggtac
     5041 aaacacacat ttacatttaa ttaaacctct tggatttaga actgatgata aaatgttaaa
     5101 aagagccggt ttagattatt gggaatttgt gaatattacg tatcatgata gtattgaagc
     5161 attttttgaa gtcacaaatg gcgagtatta cttattaaca aaatttggta aaaagactta
     5221 tagtgatttt gatttttcaa atcatgacaa agatttttac ttcatttttg gaaaagaaac
     5281 gacaggatta ccagactggg tgaaagagaa atatcaagac acagcgttaa gaattcctat
     5341 gagtgaacat attcgttcat taaatttatc aaatactgct gcgttgttaa tttatgaagc
     5401 gttacgtcaa caagattttc cgggattgaa ttaaaattta ctcattttca ttataaaaat
     5461 gatgcttaag tgctatattt agagtataag catgttaaag ttaaatggtt tgatatattg
     5521 aaagttaggg tataataatt attaatatcg ccgtttttat gtaaaatgca aactaagagg
     5581 agtgtagaaa tcatggcaat gaactttaaa gtctttgaca atagtcaact tgtagcagaa
     5641 tatgctgctg atattattag aaagcaattt aacaataatc ctactacaat tgcaggtttt
     5701 catttagata cagatcaagc gccagttcta gatgaattaa agaaaaatgt tgaaaaacat
     5761 gctgttgatt ttagccaaat aaatatttta gattatgacg ataaaaaatc atatttcgaa
     5821 gcgttaggtg taccagcagg tcaagtttat ccaattgctt atgaaaaaga tgcaatcgaa
     5881 ttaatcgctg ataagattaa aactaaagaa aataaaggga aattaacatt acaagttgtt
     5941 tctatcgatg agcaaggtaa gttaaatgtt agtattcgtc aaggactaat ggaagcaaga
     6001 gaaattttct tagtagtgac aggtgctaat aaacgagatg tagttgaaaa attatatcaa
     6061 gaaaatggta aaacaagctt cgaaccagcc gatttaaaag cacatagaat ggtaaatgtt
     6121 attcttgata aagaagcggc tgcaggttta cctgaagatg ttaaagctta ctttacgtca
     6181 cgctttgctt aattaaaaat ctgtgaggta ttgattatgt ctaaagacaa agatccaaaa
     6241 ttaaattatc atgaagaaga aaacagtatg gtaacggatt ttgaagattt aaaagaatta
     6301 ggtaaagaaa tggaacaaat ctctgatcaa aatgatcaag aaaagaattc tgaagaagac
     6361 agtcagtaaa gtattataat tgtgtaatat tcagttattt atcatgtaaa taatgtatag
     6421 tatagaacat atatcaaata ggattgatta tcttgaatat gaagttaggt agcattttgt
     6481 tacctagctt ttttattata ttcatagaag ctgactttct gctagtttca gtgttggggc
     6541 tgtttcccca acttacattg tttgtagaaa ttgaggatcc aatttttcta tgttgtggcc
//
LOCUS       DANRDI010000140         6578 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.141, whole genome shotgun sequence.
ACCESSION   DANRDI010000140 DANRDI010000000
VERSION     DANRDI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.141"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..407)
                     /locus_tag="PZ377_002222"
     CDS             complement(<1..407)
                     /locus_tag="PZ377_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDM6359084.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGE"
     gene            833..1576
                     /locus_tag="PZ377_002223"
     CDS             833..1576
                     /locus_tag="PZ377_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDM6359085.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(1803..1988)
                     /locus_tag="PZ377_002224"
     CDS             complement(1803..1988)
                     /locus_tag="PZ377_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6359086.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(1993..2331)
                     /locus_tag="PZ377_002225"
     CDS             complement(1993..2331)
                     /locus_tag="PZ377_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359087.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(2577..3185)
                     /locus_tag="PZ377_002226"
     CDS             complement(2577..3185)
                     /locus_tag="PZ377_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDM6359088.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(3671..4570)
                     /locus_tag="PZ377_002227"
     CDS             complement(3671..4570)
                     /locus_tag="PZ377_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HDM6359089.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(4669..6135)
                     /gene="msr(A)"
                     /locus_tag="PZ377_002228"
     CDS             complement(4669..6135)
                     /gene="msr(A)"
                     /locus_tag="PZ377_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HDM6359090.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
BASE COUNT         2127 a         1012 c          939 g         2500 t
ORIGIN      
        1 ttctcgccgt aaggttcaac atcgttttct tctttttcta ttttgtaaat atcacgttct
       61 gtgacaatag gtttcattaa atatagaata tttggtttat ttaaaccacg tcgtttttga
      121 atgagtagac catcgttttc taaccctttt tttatttttg ttaacttttc ttttttacaa
      181 tttaagattt ccattagctt ttcatttgaa taaacaaagt aaatgttacc atcttcatca
      241 acccagctat ttttaataga taaatttaag cgatcacgta agatagcata ggctattttc
      301 atatcattag ttagattttt ataattttca ctggtaaaga atactttagg gatttgataa
      361 aatctttctt tgtaattttc ttgcactgta aaatattgat tttgcatgac aaaaaacctc
      421 tttccatatt ttagtttggt aagaggtaat aaaacgtttt tatttagctt tacatttatt
      481 taatgattta ggtgcttgca ctaagatatt attcattata taatacaagt atcgaaaatt
      541 atataaataa acagtttcta aacgttttat taccaaaccc cgactattac cagtagttgg
      601 ggtttttgtt atattaagtt tattatagca ctttaaataa acttataaaa gggaaaatat
      661 agctttatca agggtttata aacccttgat aaatatcata aacctttcat aaacccttga
      721 taaaaaggtt gatggatgtt tatttttatt tattaatcac tttattatta agcattataa
      781 gcttataata ttttatcaag ggtttataaa cattttataa agggttgatg atatgaaaag
      841 tgtgaaaaag ttgagtgaag aattaggggt tagtaagcaa acgattttta ataatattaa
      901 gcgattgaat atagagacaa taaaacagga aaacacttcg tttattaaag aagataccga
      961 tatagaaaaa atcattcaaa gagtaaatga aaataaaaag aaatatggtt ttgaaagtac
     1021 tactgaagac aaacaaaaaa aagggtcgga taatattaat tttgaatcga aaagcgatgc
     1081 tcaaatagta gaaatcctta aaaatcagat tgatacttta aataatcaaa ttgaaaaaca
     1141 agaaagtcgt catgaaacaa caattgaatt ttacagaaaa gagttgcaag aaagatcaaa
     1201 attgcttgaa aatcagcaag ttctggcatt agagagtaat aaaaagattc aaaaattaga
     1261 aaatcaatta gaaaaagaaa aacaccttaa ttatcccttt gatacatcta ctaacactag
     1321 acaaaacgtt gatgcacaag aaaaaacata tactactaat cctgtaaata ttaacagaga
     1381 tcaggaagaa actaaagaaa cagagattca atataaggat atatctggaa gtcaaagcga
     1441 cgaaagtaca caaggtgaag aagcaaaaaa agaagacgtt ccagctaatc taaatgacaa
     1501 tgatagtgat atagaagaaa agagtgaaga aacggaacct aaaaaggggt tttggagtcg
     1561 tttgtttggt agctaaaata attgattagg cactaaaagt tataaaattt tagtgtcttt
     1621 tatatagtga gaacttctgg taatcatcga ttatgtaaac taaagtattt caaataatta
     1681 actgtattaa tactctcatc atgattgcgt tataaacgtt cgttattgag acaaaattta
     1741 atagtactac tacacaaaaa gagagtgcga attcgcactc tctttttgtg ataaatgaat
     1801 ggttaagaaa ataattcatc cacagttgta tcaaacaatc ttgccaactt aaatgctaat
     1861 gtcaatgtag gatcatattt attgttttct attgcattaa tagtttgcct agacactttt
     1921 aattctttac ttaaagtttc ttgagaaatt cctttttgtt ttctatattc tctaattcta
     1981 tttttcatta atttaacctt tagatgtata ttttttattt gtagtatagt aattaaacag
     2041 cataagaaat agtgctacaa ttataaagaa aatttctgga tattgagaaa accaagatcc
     2101 ttcaatactt ggtgacaaac ctgtaaattt aagcactagt gtaaataaca tacaatatac
     2161 aatccaaata aaacttttag ccagtatttc aataaacatt ttacgttgtc tttcatcgta
     2221 tcgatcttgt tgtccttttt tatctaatgt ttctgaaggt aaatatttgt tagttatagc
     2281 cgtccataaa caagaaaaaa ttaaaataat aataaaagga attaaattca ttataacacc
     2341 tcataaatgt aaaaagtatt tgacatttat gagtgtaaat atttttgata caaatgtcaa
     2401 atacttttta cattataata tattttaaaa caccttatta gtaaaacaat tactatatag
     2461 aaagctgcga actcgtactc ttttttcttg tataattatt aaaatctcaa ttcaataata
     2521 tgttatggaa tttgagacac tgtgagagac gctcatttgc attgacaatc aatttatcaa
     2581 gataatccct tatcatgttt aattctataa actgtttgtc ttgtgatatt aacttctttc
     2641 gcaatcttac taatcgcttt gccttcttct aacatttcca ccacgcgatg ataaataaca
     2701 cgtttctgtg ggtctttagc atttggtgaa taaagtaaag ggcgcccttt atatacgcct
     2761 ttttccttcg caacttgaat cccttgcgct tgtcgacgtt tactttcatt tctctcttgt
     2821 tctgaaacca ttgctaatat ttgaatgatt aaatctttca taaacttatc cagtaatgga
     2881 ttgccaatta cttcattcat cataggtaag ctggtaatca ttaactgaac gtctttttct
     2941 tttaaataat tcacagtttc aataatttca tcatagttac gtcctaaacg atcaatagat
     3001 tccacaacga atcgatctcc cattctgaca aaattgagtg cttcttgaaa tacaggtcta
     3061 ttttccactg actttcccga ctgtttctct gtaaagattt tctccacacc aaacgtcttt
     3121 aaattatcca attgtctttc taaattttga tctatcgatg atactctcgc ataacctata
     3181 atcattcatc ttcactccat aataaatata caccctaatc atacgtttta attgttataa
     3241 tttcaacaat tctttttgta tggttagggt gtactcaaag tgtacatatc tataattaaa
     3301 acttactaaa aataaatata gattttttct ttgtaacaga accgttcatt tgaaggtcat
     3361 ctctatcata aatatttaaa ttaactaaaa attttaatca tctgtttaat acttatgaaa
     3421 tgtatttacg gtaggtatta aaattgatga aatttatata agtgctttca atgaaaatga
     3481 ccgtttattt atcctcctga tttaaggagt tagaaatttc gaaaacgatt ctttccattt
     3541 gttgcatttg ttccatgtcc agatatttaa tacattatag aaaaccaaag accatcacta
     3601 attaaacgca gttttaaaat ttcatcagga ttgccttcgt ctttaaaatg ggcctctttt
     3661 aatataagtt ctactctttc atacctaact ctttcaatgc catcgtttca tacgccgatt
     3721 ctcctgattc catagcaaac tcagcgataa acattgggaa tactgcattg agttctatta
     3781 tatgctcttt cattcgtggc catatttcac ctccagcttt accgtatgct gttataagtt
     3841 gctctaatcc ttcgtcgtcg aatacacgat ggtgtcctat aaagtccatt gatgggtcgg
     3901 agtgggttgc ttcagtccag tctatgagac ctgtgacgtt tgcttggtta tctaccatta
     3961 tatgtcctgg atgtaagtcc ccatgtatca tggtcgcatg tcgaggccat agttcgtcgt
     4021 tttctaacca ttgtttccat ctgttccata attcatctga tacgccataa gtttctttaa
     4081 ctttattcat tcttctttga aagtcatttt ttatttcttg tatggttttg atatttatat
     4141 gctgaacgtt aatgttttct tctggtatgt tgtgtaaatc tacgagtgtt tcagctaatg
     4201 tgttaataaa gttttctggt aatggtttgt gttcaatttc ccatacataa ttttgtattt
     4261 ctggatctat tgtggctgcg ggtttacctg taagttttgg gtaagcaata aggtcttttg
     4321 catgtacttt ccacttcggt atttcaaatg aaacattctt ctgtaagaag tctaccgttt
     4381 gtttttcggg ttttgttcgt ttataaacgt caggtcttct tggtagtctc aaaacccatt
     4441 ctactccatg tttatctttt ccaaatgcaa cttgaaaatc aagtcccgat tcattcaatg
     4501 agattgtttg aggttggatg tgtaattgat atttttctgc acatttaata atttcattat
     4561 gtcgagtcat tttattggat ccttatttct attatatttt tcttgtgaac gtatttttta
     4621 tattgatata aatgaaccta atattcaata taaattagtg tatagttttt aagttatatc
     4681 atgaagagat tgtcctgtta attcccattt tttatctgat acatgtttaa caaaccttgt
     4741 atcatgtgat gtaaacaaaa tgattccagg atatttattc ataaacattt ctaatgcttc
     4801 taatgtttta atatctaaaa aattagttgg ttcatccaaa attaacatat tcgctttcgt
     4861 tgaaaataat actgctaacg ataatttcgt tctttcccca ccactcaaaa cattacaaga
     4921 acgatcaagt gcttcattta aacccaagtt atttaaaata gctctactga atgattctga
     4981 tgaatccgtt tcatccatta aatattgcaa taatgaaacg tcacgcatgt cttcataagc
     5041 aagttgacga tagtatgcca tttgcacttt aggagaacaa tcaattccct ctatttggtg
     5101 gtaaatagct tcaagtaaag ttgtcttacc tacaccattt gcacctacga gcgctatatt
     5161 tttgccatat ggtatttgga atcgtacttg tgttagcagt ttttgacttc ctttaaccaa
     5221 tgttagattt tgtgcaatga ttggataatt attatggata tcataaattt tattttgtgg
     5281 gaaattgaat tcatgataac tttgtggttt ttcaacttct tccaaatgtt ccattctttt
     5341 ttcaatatgc ttagcttgtt tttgagcagc cttctcaacc gtgccttttt gttttgatgc
     5401 acttaaacga tctggtgcta tacttttgtt tttttgtttt gatgatgctt gtgccatttg
     5461 ttgcgcttga tttcgtttag ctttactggc ttgggacaat ctttgttttt cacttatata
     5521 ctgttcatat tgacgttgtt gttctaactg ttcttgttca tattgatttt gatactgtgt
     5581 gtaattacct ttaaacactc ttattgtgcc atcttcttgt atatcccaaa ttgtgtcagc
     5641 aatttggtct ataagtgctc tatcatgact tactataatt aaagtaccat agtaatattt
     5701 taaaatattt ttcagataat ctttaccaat tttatccaag tgatttgtag gttcatctaa
     5761 aagtaatatc ggactataat ttgatatgac attagctaat ttatattttg ctttttcacc
     5821 accactcata ctgtcggttg tatgcatagg tatatggagt tcactcatta aactaccgtc
     5881 taacgtgtta aaatcatttt ccacatccat gttaagctgt tcaaaatatt gaatttcgcc
     5941 aaccgtttca actgttccag attctggttt tgttttttga gcaatcatat ttagtaatgt
     6001 ggttttgcca gtaccattac ccccaataag tgctattttt tcaaattggt aagcgtaaag
     6061 atgaccgatg ttaagtgatc gtaaatctgt caatttatga ttgatttggt taaatttaat
     6121 tgtatattgt tccatattta tacactcctt gtatatttaa aggacgtgta cgcaaaaaaa
     6181 tgagacaggc tactcccgtc tcattcacat tacactttca aacacttaaa aatttattat
     6241 gatgttttag tatttgaaag cgtttattat tcctatcgaa ggataaaaaa tgacataagt
     6301 catcctatga gtgtgtattg cggacaagcc tttttgaatt aatttagttt tgtattcaaa
     6361 aaggtttatt tgagtctcat agaagctgtc attttaatcc tccttcatcg ataaaatagt
     6421 aatgttaata taacattttg ttggtttttt tacaatatat ctctaagtag aaagattata
     6481 taacaaaagt ataaaaatac aattttagat tgattataca tctccttaat ttagagtgta
     6541 ctcataaaat acggttctgt tgcaaagttg aatttata
//
LOCUS       DANRDI010000141         6559 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.142, whole genome shotgun sequence.
ACCESSION   DANRDI010000141 DANRDI010000000
VERSION     DANRDI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.142"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..606)
                     /locus_tag="PZ377_002229"
     CDS             complement(<1..606)
                     /locus_tag="PZ377_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDM6359091.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGI"
     gene            complement(1040..1810)
                     /gene="sigB"
                     /locus_tag="PZ377_002230"
     CDS             complement(1040..1810)
                     /gene="sigB"
                     /locus_tag="PZ377_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDM6359092.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            complement(1785..2264)
                     /gene="rsbW"
                     /locus_tag="PZ377_002231"
     CDS             complement(1785..2264)
                     /gene="rsbW"
                     /locus_tag="PZ377_002231"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDM6359093.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(2266..2592)
                     /locus_tag="PZ377_002232"
     CDS             complement(2266..2592)
                     /locus_tag="PZ377_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDM6359094.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(2711..3712)
                     /locus_tag="PZ377_002233"
     CDS             complement(2711..3712)
                     /locus_tag="PZ377_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDM6359095.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            complement(4062..4424)
                     /locus_tag="PZ377_002234"
     CDS             complement(4062..4424)
                     /locus_tag="PZ377_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDM6359096.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(4421..4591)
                     /gene="mazE"
                     /locus_tag="PZ377_002235"
     CDS             complement(4421..4591)
                     /gene="mazE"
                     /locus_tag="PZ377_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDM6359097.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(4676..5824)
                     /gene="alr"
                     /locus_tag="PZ377_002236"
     CDS             complement(4676..5824)
                     /gene="alr"
                     /locus_tag="PZ377_002236"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDM6359098.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(5890..6249)
                     /locus_tag="PZ377_002237"
     CDS             complement(5890..6249)
                     /locus_tag="PZ377_002237"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDM6359099.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(6253..>6559)
                     /locus_tag="PZ377_002238"
     CDS             complement(6253..>6559)
                     /locus_tag="PZ377_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDM6359100.1"
                     /translation="VFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPYFRIQ
                     NIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNKNEE"
BASE COUNT         1964 a         1266 c          837 g         2492 t
ORIGIN      
        1 aatacctttt tcatcttcag catttttctt tttagatgta gttaacacac cttgatgata
       61 catatctttt aaaatttttg ttctatattt aggattatct gaaatttgtt ccgcaataat
      121 atcttgtgca cctttgatag catcttcaac cgattgcact tcttcattta taaattgttg
      181 tgctttttct tcaattgaga cttcatgttt acgtgccttc atccatatcg ctaatggctc
      241 taacccttta cgtttcgcct cagttgccct tgtctttttc ttttgtttaa aaggcctata
      301 taggtcttca acacgttgta atttgttctg ttttaaaata tccttcttta attcctcagt
      361 aagtaatcct tgctgttcta tatttttgat aacttcttct ttacgttttt gtaaattgac
      421 catatattgg tattcgtcat caatttgctt tatttgaact tcatctagtc caccagtttg
      481 ctcttttcga tacctcgcaa taaatggtac tgtatttttt tcttctagca gtgttaatac
      541 tgcttcaatt tgttttttac taaattgata tttctctatg attgaattaa tcaattgatt
      601 gtccatttac ttaacccaca ctttttctta aatttactca tttattttaa catattctaa
      661 aatacttcta ttaaggtatg atacttaatg taattttcac ttccaaaaca tttaaaacga
      721 atgattaggt catactatat tttaaaacgc actaagatta ttgaaaatga cacaccatca
      781 tttctaaatt tcataatcta agactacttt ttttattcta atagcaaccc acctatttaa
      841 aacgacaatt agatatcttc ttcacttaaa ttattaatac tatatcgcga catttatgtg
      901 gatacacatt aaactacact aataatgtat taattcgaaa atcttaatta aattaaaaat
      961 acccctcgat ttcaatttaa taatcaaggg ataattatat ggatttttca ttataaaacg
     1021 tatcattaat aaacaaattc tattgatgtg ctgcttcttg taatttctta attgccgttc
     1081 tctgaagtcg tgatacatgc atttgactta aaccgatacg ctcacctgtc tctttttgac
     1141 tcaatccttc aataaacgta cattgtatga tttctcgttc gcgatcagat aatataggta
     1201 atattttttc taaaatcata cgtttttcag ttaagtcata atggtcatct tgttgcccca
     1261 taatatctaa tagcgtaaca gttgaaccat ctttatcagc ttcaatggaa tgatcaacac
     1321 ttaacgcatt ataactttgt cccatttcca ttgcttctaa cacttcttct tctgagactt
     1381 ctaatcgatc agctatttca ctgatagaag gtgaacgctc taattcagcg gttagttcat
     1441 cgctcacttt tttgattctt ggcccaattt ctttaatacg tctcggaaca tgtacactcc
     1501 aagttttatc tcgtagatat cttttgattt caccgattac agtaggtact aaaaaggctt
     1561 caaacttccg ttcaaaggac atatcgaatc tatttatggc acctattaaa ccaaccatac
     1621 caacttgaac taaatcttcg tgatgtgatt gtcctttaga atatttatat gccaatgact
     1681 caattagttt ttggtaatgt ttaactaact tatcctgtgc atctgtattc ttattttctt
     1741 ggtgttcttt aatccattgg ttaatttgct caggtgaaat ttcattagct gatttcgact
     1801 ctttcgccat tatttcgcac ctgctctttt tttatatact tagtcatact gattgtcaca
     1861 ccagattctt tatatactgt gacttcatcc attaaagatt cgattaaaaa taaacctagg
     1921 ccaccttcgc gtaaaaagtc tatattttcg tctttatcgt aaggacctat ttttgattta
     1981 gttgtttcat aatcaaaact gtcaccttta tcagaaataa caattttaat tttatcttct
     2041 aaaatttcaa aatatatgtt aataatgccc acattgttat tttctttgta tgcatgttta
     2101 actgcatttg tcacagcttc actaactgca atcttggcat cttcaatatc atcatatgta
     2161 gcaccagctc tcgaaaaaac gccagaaagt gttaaacgaa ttaaacttac atactctgcc
     2221 gatgctggca cgcgcatttc gataaaatct tctttagatt gcatgttatt cgacctccgt
     2281 tccttcatta acatgcatta aatccttaag accagtaatt tcaaatagtc taccgatacg
     2341 atctgacaca cctaaaatgt atagttcttt atcattttgg tttaatgctt ttaatgtacc
     2401 tacgaataaa cctaaacctg tcgaatccat ataactcaca ttttctaaat taacataaat
     2461 atcacgagtt ccatcttgtc tcataggtgt taaaacctct tctaattcag gcacagtata
     2521 aacatctaat tctccaccga ctttaacttc gtaaaattta tcttgagtgg ttgtttctat
     2581 attaagattc atttcattac actcctactt attaaaaata tttatcttca ttttaagcgc
     2641 tgtatccacc atatttatat ttcaaattat tatataccca tcgaaataat ttctaatctt
     2701 cttttttaaa ttaatttact ctttttataa tcaaaatagt catatcatct tttttatttg
     2761 ggttttgaag ctttaaaatt gcttcataga taatttgaac aatatcttgt gggtgcatat
     2821 gtttatgttt tttaatatat tctaaaagtt tttgtttatc tataaaggta ccttcactat
     2881 ttctagcttc agtcacacca tccgttaaaa tgataattaa atcatcaagg tatattggaa
     2941 tttcttgttg ttgatatcgt gtttgtgaac tgattcctaa cactctacct ctaactgaaa
     3001 tttcttcaaa ttcttctttt tcagcgcgat aaatatatcc aggctcatga ccagctgaac
     3061 tacaatacaa taaatggttc atttcttcat ataaaccata aaacattgtg acgaacatat
     3121 tttgattaat attcttttca acaacacgat ttaaacgttt taaaccatca ctcggtaatt
     3181 gtgagtgtcc ataagaatcc atgccaaact ttatcatact cattgctaaa gcagctggta
     3241 taccttttcc aatgacatct gcaacagcaa agctcattgt gccatcgtta tggtcaatta
     3301 aattaaaata atctccactt actttttgtg ccgccactga aataacgcca atttgaatac
     3361 tatcaaattg tggaatatct gttttaagca ttgtttgttg taagctagaa gctaagtcta
     3421 tctctttatc gtgaacttga agtttatcta ccaatctttg ataatctcga taactataac
     3481 caaagccttt aacgatttct tgtaagacat ctaatgtctt gaacacatct tcacgcgtta
     3541 agtttaacgt caatatatag tttttgtgaa tatcgacaat gtcttcaggc aagacgtcct
     3601 tacgaatcac ttcgtcagtg tatttctcac acttttttat caattctact ttatcttggc
     3661 acgttaaact ttcatcaatt aaacccttat aatgttgctt aaattcttcc acgattagtt
     3721 gcctcctcac ttcccttaca aagcaaatgc ttaagcaagg ataattatga ttcacttact
     3781 aacatttatt taattgcact tatcaatttt gtcgtttgca attttgctgt agatgatact
     3841 tttaagctaa aaaaaacttt aggatggaat acctaaagcc taaaaaactt aagacatcct
     3901 tttataataa aaaatatgaa tatcataact ggtatttgct aaaatctttg tttataagaa
     3961 aattcttcgt acagaattta gaaatagtta taattattat aaagttattg tcttttatat
     4021 caatatttta tttatctctg aaaaatacat ataatagacg cctaattttt ctggtgagct
     4081 actgcattca gccctaaact aatcattagt gcattatcta cttctttcat tttatcatcg
     4141 gataagtacg tcagtttttc tttcaatcgt tttttatcaa gtgtacgaat ttgttctaat
     4201 aatataactg agtctttatc caacttatac tttttctttt caatctctac atgtgtcggt
     4261 attttcgctt tattaatcct accagttatt gccgcaacaa taactgtagg actatattta
     4321 ttaccagtat cattttgaat tatgactaca ggtctgactc ccccttgttc agatccctgt
     4381 actggtgata aatctgctaa ataaacatct cctcgtctaa tcattcattc gttgaattag
     4441 aagataaata tgtttcgttg caatcgcatg cttcacactc tatcggaaaa gcttcgttcg
     4501 ctagggagag atttaaatca gccatttgtg aatatccttc ttttaaagat tgttctaagc
     4561 tatgacttct attttgacta aaagataaca tatgagaacc tccaattgat tccaaaagtt
     4621 attaaatgac ctttaattac tctaatgata acaaatttta tatgcactga ccatattatt
     4681 ttaacaattc atttgttaca aaacgttgat caccatcatg atagattcgc ggcaaacgtc
     4741 tcgacaagtt acaaagcact tcataattaa tagtatgttg cttttcagct accacctcta
     4801 ccgactgtgg actttctcta tgattatcta ttaaaatcac cgaatctcca gctttaactt
     4861 gatctggcac tttaacaatt gtctgatcca tacatacgcg accaataact tcgcattgat
     4921 gaccatttac atttacaaag ctaccttgca ttatgcgtaa atagccatct gcatatccaa
     4981 taggtaacaa tgctattgta gttgggtcag tagctgtata agttgcacca taacttacag
     5041 actcacccgc ttgtagcgtc tttgtttgaa ctacattagc aattaattgc acacttggtt
     5101 taaggtgtac tttaactttt tgctgtacat actctgatgg ataatatcca taaagggaaa
     5161 ttcctggtct tattgcatta cagaattggc aatccattaa tagagagcct gctgagttct
     5221 gacaatgtat atattcaggt ttaattgctt cattgaccat atctttaaaa cgttgatatt
     5281 gttcagttgt catatctcct ggttcgtcag cacaggcaaa gtgtgtaaac acgccttcaa
     5341 atacaagttg ctcatattgt tgaatgattt caatcacttc ttgatacgtt ttagtatctt
     5401 taatacctaa acgtcccatt cctgtatcta atttaatgtg caaccataac tttttctctt
     5461 gctcaccaga aatgttttta attgcttctt tcaaccactg tttagacgga accgttaagg
     5521 caactcggtg ttgtatcgct ttatcaatat ctttagctgg taacacacct aagactaaaa
     5581 ttttagcagt aatcccatgc attctaagtt ctatcgcttc atctaacgtt gctacagcaa
     5641 aaaatgtggc gccattttcc attaaatgac gtgctacttt aacactacct agtccatagg
     5701 cattggcttt aacgacagcc atcactgttt tatttggatg caatgtactg aatactttga
     5761 aatttgatgc aacagcgttt aaatctacat tcatatacgc agatctataa tatttatccg
     5821 acatattact tcctcctgta attcccacac gttttaaaac tagatcttaa ttatcattgt
     5881 ataacaaatt taaaatgctg acttttctaa aacaacttgg ctcatcgcat aatgctcagt
     5941 gtgtgagata ctaacatgta cgataaaccc ttcgtaatca atctttggtt tgccaagttc
     6001 gtcgttgtaa cagtctatat cgttaaaagc tacatgtttt cctaagcctg tgcctaatgc
     6061 tttactgaac gcttcttttg tagcaaacct gccagctaaa aattcaattt tacgttgctc
     6121 atgtgtgaaa ttgttgaatt tgtgctgttc atttttagtt aaaatccgct caaccaattt
     6181 tggttgctta ctatataacg cttgtattcg atcgatttcg attaaatcta caccaattcc
     6241 atgtatcatt gattattcct catttttatt gttttctaaa tgtctctcgt tatttaacca
     6301 tcgtttcaat ctacgtgctt cacttctatc tattaattca atttcagctt gaccacccgc
     6361 tgtataaagg gtcaaagttg ctaatcgata gcgtctcatt ataggaccaa catcgatatc
     6421 aatattttga atacgaaaat atggtattac cttttcatcc aaaaataaaa tgccgtttcg
     6481 tacacgcaaa tggtgttttt caaatgcata tctgcagtgc ttatatcgat agactggcgc
     6541 tataacaagc gtgaaaaca
//
LOCUS       DANRDI010000142         6516 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.143, whole genome shotgun sequence.
ACCESSION   DANRDI010000142 DANRDI010000000
VERSION     DANRDI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.143"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            53..370
                     /locus_tag="PZ377_002239"
     CDS             53..370
                     /locus_tag="PZ377_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359101.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            363..665
                     /locus_tag="PZ377_002240"
     CDS             363..665
                     /locus_tag="PZ377_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDM6359102.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            670..930
                     /locus_tag="PZ377_002241"
     CDS             670..930
                     /locus_tag="PZ377_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDM6359103.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            944..1306
                     /locus_tag="PZ377_002242"
     CDS             944..1306
                     /locus_tag="PZ377_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359104.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            1303..2469
                     /locus_tag="PZ377_002243"
     CDS             1303..2469
                     /locus_tag="PZ377_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDM6359105.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            2495..3052
                     /locus_tag="PZ377_002244"
     CDS             2495..3052
                     /locus_tag="PZ377_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDM6359106.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            3120..5072
                     /locus_tag="PZ377_002245"
     CDS             3120..5072
                     /locus_tag="PZ377_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDM6359107.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            5085..5270
                     /locus_tag="PZ377_002246"
     CDS             5085..5270
                     /locus_tag="PZ377_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDM6359108.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            5270..5671
                     /locus_tag="PZ377_002247"
     CDS             5270..5671
                     /locus_tag="PZ377_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HDM6359109.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            5671..5928
                     /locus_tag="PZ377_002248"
     CDS             5671..5928
                     /locus_tag="PZ377_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDM6359110.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            5931..6131
                     /locus_tag="PZ377_002249"
     CDS             5931..6131
                     /locus_tag="PZ377_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359111.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            6146..6388
                     /locus_tag="PZ377_002250"
     CDS             6146..6388
                     /locus_tag="PZ377_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359112.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            6403..>6516
                     /locus_tag="PZ377_002251"
     CDS             6403..>6516
                     /locus_tag="PZ377_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDM6359113.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQA"
BASE COUNT         2524 a          888 c         1336 g         1768 t
ORIGIN      
        1 tttatcaaaa tatacaactt aattaaatca aaatatacgg aggtagtcaa ctatggctga
       61 aaatattaaa actgaacaac attattacac taaagatttc tcaggataca gaaatgaaga
      121 agataacttt gtagcaaatc aagaattgac agtaacaatc acattgaacg agtacagaaa
      181 acttattgaa ataaaggctg ttaaagataa agaagaagat acttacagag gtaagtattt
      241 tgcggaagaa agaaaaaacg aaaaattgga aaaagaaaat ataaaactaa aaaacaaaat
      301 ttatgaatta caaaacgaag aagataacga ggaggacgaa gaaaacgagg aggaagtcaa
      361 caatgactaa aaaatataaa gacatgactc aggaagaagt aaaagactta ttatctgaaa
      421 aaagcggaga attgtatgaa ttagcgaaag aaattaaggg agaaagtaaa tttgatattt
      481 tgcttttctc atcaatagga gttatcgacg gagattattt agcaggttca agttctgtga
      541 ttggtcatac tttcgatctt gcttccttat tggatagcac taagagttat aaagacattg
      601 tcaatgttct ccaaatgtgt aaatcacaaa aatttcacgg tattgatgat aacaaggagg
      661 gctaaaacaa tgtattacaa atttggtgag ataaaaaata aaattatcag ctttaacggg
      721 tttgaattta aagtgtctgc gatgaaaaaa catgacggta tcagtataca aatcaaggat
      781 atgaataata ttccacttaa atcatttcat gttgtagatt taagcgaact atattttgcg
      841 acggatgcaa tgcgtgacgt tataaacgaa tggattgaag agaacacaga tgaacaggac
      901 agactaatta acttagtcat gaaatggtag gaggatttaa tcaatggcaa tattagaaga
      961 tatttttgaa gaattaaaac tattaaataa caatttacgt gtgttaaaca ctgaactatc
     1021 aacagtagat tcatcaattg tacaagagaa agttaaagaa gcaccaatgc caaaagaaga
     1081 aacagctcaa ctggaatcaa ttgaagaagt taaggaaact tctgctgatt taactaaaga
     1141 ttatgtttta tcagtaggaa aagagttcct taaaaaagca gatacttctg ataagaaaga
     1201 atttagaaat aaacttaacg aacttggtgc ggataagcta tctactatca aagaagagca
     1261 ttatgaaaaa attgttgatt ttatgaatgc gagaataaat gcatgaagct agatcactca
     1321 aatagagctc atgcaaagct tagtgcaagt ggagcaaaac aatggctaaa ctgcccaccg
     1381 agtattaagg caagtgaagg tattgcagat aaaagtacag tttttgccga agaaggtaca
     1441 ttcgcccatg agttaagtga gttatatttc agccttaaat atgaaggcct aacacagttt
     1501 gagtttaata aggcttttca aaattataag agaaatcaat attacagcga agaattgcgt
     1561 gaatatgtgg aagagtatgt agctaatgta gaagaaaaat ataacgaagc tttgagtagg
     1621 gataatgatg taatagcttt atttgaaaca aaattagatt taggtaaata cgtccctgaa
     1681 tcttttggaa ctggtgatgt cattatattt tcaggtggtg tacttgaaat tattgacctt
     1741 aaatatggta aaggcattga agtttcagct atagataatc ctcaacttag attatatggc
     1801 ttgggcgcat atgaactgct tagtttaatg tatgacattc atacaattcg catgactatc
     1861 atacaacctc gaatagataa cttttctact gaagagttac caatatcaag attacttcaa
     1921 tggggagccg attttgttaa accattagcc agacttgctt ataacggtgg aggagagttt
     1981 aaagcaggta gtcattgtag attctgtaag ataaatcatt catgtagaac acgtgcagaa
     2041 tacatgcaaa atgtgcctca aaagccacca catttgttaa gtgatgaaga gattgcagaa
     2101 cttttatata aactgcctga tatcaaaaaa tgggctgatg aagtagaaca atatgcacta
     2161 aatcaagcga aagaaaatga taaaaactat cctggttgga agcttgtaga aggtcgttca
     2221 cgaagaatga taactgatac aaaagcaatg cttgaaaagt tagttgaagc gggttataaa
     2281 cctgaagata ttacagaaac caagttactt agcattacga atttagaaaa attaattggt
     2341 aaaaaagcat tttctaaaat tacagagggt tttatagaaa agccgcaagg taaattaaca
     2401 cttgctaccg agtcggataa acgaccagct ataaagcaat ctgctgaaga tgattttgac
     2461 aaactataaa aatttaaaag gacggtatat aaacatgaaa gcaaaagtat taaataaaac
     2521 taaagtgatt acaggaaaag taagagcatc atatgcacat atttttgaac ctcacagtat
     2581 gcaagaaggg caagaatcaa agtattcaat cagtttaatc attcctaaat cagatacaag
     2641 tacgataaaa gccattgaac aagctataga agctgctaaa gaagaaggaa aagttagtaa
     2701 gtttggaggc aaagttcctg caaatctgaa acttccatta cgtgatggag atactgaaag
     2761 agaagatgat gtcaattatc aagacgctta ttttattaac gcatcaagca aacaagcacc
     2821 tggtattatt gaccaaaaca aaattagatt aacggattct ggaactgttg taagtggtga
     2881 ttatattaga gcttcaatta atctatttcc tttcaacaca aatggtaata agggtatcgc
     2941 agttggattg aacaacattc aacttgtaga aaaaggcgaa cctcttggcg gtgcaagtgc
     3001 agcagaagat gatttcgatg aattagacac tgatgatgag gatttcttat aagtcaatag
     3061 gtggggtttt agccccactt taattttaaa gaaattgagg tgtcaagaat ttgagattta
     3121 tgaatataga tattgaaaca tatagcagta atgatatttc gaaatgtggt gcctataaat
     3181 acacagaagc tgaagatttc gaaattttaa ttatagctta ttcaatagat ggtggagcga
     3241 ttagtgcgat tgacatgact aaagtagata atgagccttt ccacgctgat tttgagacgt
     3301 ttaaaattgc tctttttgat cctgctgtaa aaaagtatgc attcaatgct aatttcgaaa
     3361 gaacttgtct tgctaaacat tttaataaac agatgccacc tgaagaatgg atttgcacaa
     3421 tggttaattc aatgcgtatt ggcttacctg cttcgcttga taaagttgga gaagttttaa
     3481 gactacaaaa ccaaaaagat aaagcaggta aaaatttaat tcgttatttc tctatacctt
     3541 gtaaaccaac aaaagttaat ggaggaagaa caagaaactt gcctgaacat gatcttgaaa
     3601 aatggcaaca atttatagat tactgtattc gagatgtaga agtagaaatg acgattgctc
     3661 ataaaattaa agactttcca gtaactgcaa ttgaacaagc atattgggtt tttgaccaac
     3721 atataaacga cagaggtatt aagctttcta aatcattgat gttaggagct aatgtgctcg
     3781 ataagcagag taaagaagaa ttgcttaatc aagctaaaca tataacaggt ttagaaaatc
     3841 ctaatagtcc tacacaatta ttggcttggt taaaggatga ccaaggatta gatataccta
     3901 atttacaaaa gaaaacggtt caggagtact taaaagaagc aacaggaaaa gctaaaaaaa
     3961 tgctagaaat tagattgcaa atgtctaaaa ccagtgtgaa aaaatacaac aaaatgcatg
     4021 acatgatgtg cagtgatgaa cgggtaagag gtctgtttca attttacggt gccggtactg
     4081 gaagatgggc aggtagaggt gtacaacttc agaatttaac aaagcattat atttcagata
     4141 ctgaattaga aatagcaaga gatcttatta aagaacaacg ttttgacgat ttagatttat
     4201 tactcaatgt tcatcctcaa gacttattaa gtcaattagt taggacgaca tttactgctg
     4261 aagaaggtaa tgaactagca gtaagtgatt tttctgcaat agaggcaaga gtcatagcat
     4321 ggtatgcaaa agaacaatgg cgtttagatg tgttcaacac acacggaaag atatatgaag
     4381 catcggcttc tcaaatgttt aatgtcccgg tagaaagcat aactaaaggc gaccctctca
     4441 gacaaaaggg aaaagtgtcc gaattagctt taggttatca aggtggcgct ggagctttaa
     4501 aagcgatggg cgcattggaa atgggcattg aagaaaatga attacaaggt ttagttgata
     4561 gttggcgtaa cgcaaatcct aacatagtta atttttggaa ggcttgccaa gaggctgcaa
     4621 ttaatactgt gaaatcccga aagacgcatc atacgcatgg agttagattt tatatgaaaa
     4681 aaggttttct aatgattgaa ctgcctagtg gaagagcttt agcttatcca aaagcttcag
     4741 ttggtgaaaa tagttggggt agtcaagttg ttgaatttat gggcttagat cttaaccgta
     4801 aatggtcaaa gttaaaaacg tatggtggga agttagtcga gaatattgtt caagcaactg
     4861 caagggattt acttgcgatt tctatagcta ggcttgaagc atcaggtttt aaaatagttg
     4921 gacatgtcca tgatgaagta attgtagaaa tacctagagg ttcaaatgga cttaaggaaa
     4981 tcgaaactat catgaataag cctgtcgatt gggcaaaagg attgaatttg aatagtgacg
     5041 gatttacttc tccgttttat atgaaggatt aggagtgtga ttgaatgcaa catcaagctt
     5101 atatcaatgc ttctgttgac attagaattc ctacagaagt cgaaagtgtt aattacaatc
     5161 agattgataa agaaaaagag aatttggcgg actatttatt taataatcca ggtgaactat
     5221 taaaatataa cgttataaat atcaaggttt tagatttaga ggtggaatga tggctagaag
     5281 aaaagttata agagtgcgta tcaaaggaaa actaatgaca ttgagagaag tttcagaaaa
     5341 atatcatata tctccagaac ttcttagata cagatacaaa cataaaatgc gcggcgatga
     5401 attattgtgt ggaagaaaag actcaaaatc taaagatgaa gttgaatata tgaagagtca
     5461 aataaaagat gaagaaaaag agagagaaaa aatcagaaaa aaagcgattt tgaacctata
     5521 ccaacgaaat gtgagagcgg aatatgaaga agaaagaaag agaagattga gaccatggct
     5581 ttatgatgga acgcctcaaa aacattcacg tgatccgtac tggttcgatg tcacttataa
     5641 ccaaatgttc aagaaatgga gtgaagcata atgagcataa tcagtaacag aaaagtagat
     5701 atgaatgaaa cacaagacaa tgttaaacaa cctgcgcatt acacatacgg cgacattgaa
     5761 attatagatt tcatcgaaca agttacggca cagtatccac cacaattagc attcgcaata
     5821 ggtaatgcaa tcaaatactt gtctagagca ccgttaaaga atggtcatga ggatttagca
     5881 aaggcgaagt tttacgtcga tagagtgttt gacttgtggg aggggtaacg atggcaacgc
     5941 aaaaacaagt tgattacgta atgtcattac aggagcaact ggaattagaa gactgcgaaa
     6001 aatatacaga cgaacaagtt aaagcaatga gtcataaaga agttagcaat gtgattgaga
     6061 actataagac aagcataagg aatgaagaac tatattacga atgcatgtcg tttggactgc
     6121 ctaattgtta aaaggagtga cgaccatgac agatagcgca cgtaaagaac gcttaaacca
     6181 atttttcggc tctaaaagat atctgtatca ggataacgaa cgagtggcac atactcatgt
     6241 agtaaatggc acgtattact ttcatgggca tatcgtgcca ggttggcaaa gcgttaaaaa
     6301 gacatttgat actgctgaag agctcgaaat atatataaag caacatggtt tggaatacga
     6361 ggaacagaag caactaactt tattttagag gagatggaaa tgatgaataa ccgcgaacaa
     6421 attgaacaat caattatcag tgctagtgcg tataacggca atgacacaga gggattacta
     6481 aaagagattg aagacgtgta taagaaagcg caagcg
//
LOCUS       DANRDI010000143         6468 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.144, whole genome shotgun sequence.
ACCESSION   DANRDI010000143 DANRDI010000000
VERSION     DANRDI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.144"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(287..1933)
                     /locus_tag="PZ377_002252"
     CDS             complement(287..1933)
                     /locus_tag="PZ377_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDM6359114.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
     gene            2137..2499
                     /gene="mscL"
                     /locus_tag="PZ377_002253"
     CDS             2137..2499
                     /gene="mscL"
                     /locus_tag="PZ377_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDM6359115.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            complement(2570..5599)
                     /locus_tag="PZ377_002254"
     CDS             complement(2570..5599)
                     /locus_tag="PZ377_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDM6359116.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQSDYEKIIELNNTITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEI
                     KSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIK
                     FVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRDLNKFIEQMKEEKDNLTLQIH
                     NKQLRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVNQHQELSKEIED
                     KLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTDDNDIDEIIAW
                     RGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGKMNTLI
                     DEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFV
                     LIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLS
                     GGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLLNLKST
                     GRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(5603..>6468)
                     /locus_tag="PZ377_002255"
     CDS             complement(5603..>6468)
                     /locus_tag="PZ377_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDM6359117.1"
                     /translation="GKERLNYGASWFEHNQLFIRTDFTSINSPIEINGVNFYTLPYAT
                     VSEMKHYFEDDTIETHQQGITRCIETIAPEIDEDAVNILISHLTVQGGKTSDSERPLT
                     IGTVESVQKGVFDIFDYVMLGHLHHPFSIEDDKIKYSGSLLQYSFSEAGQAKGYRRVT
                     INDGIINDVFIPLKPLRQLEIISGEYNDVINEKVHVKNKDNYLHFKLKNMSHITDPMM
                     SLKQIYPNTLALTNETFNYNEENNAIEISEKDDMSIIEMFYKHITDKELSDIQSKKIK
                     NILENELRKED"
BASE COUNT         2058 a         1126 c          844 g         2440 t
ORIGIN      
        1 attgatttat gtcccagcct gagttaattt tctataaaag tatatttaat ttgcgtttat
       61 accgtcaaac ttcactttag ctttgtcaaa cccctttcta ttaagttttc agaaataaac
      121 ctatcttaaa atataaaaaa atcgagaatt cgtagtttaa taacgaaatt ctcgttctta
      181 tccttttgaa tatactcaat tttccacaaa aacaaacaag tagtatatct gttctagcta
      241 ctagaatgac atactacttg ttattaaaat acttaactaa actttattag ttatcttttt
      301 tctctatatt tctacgtgac tgacgctttt caagaatgtc agattcataa tcttcttgtt
      361 gactcttgat atattcttgt aagcgatgtt tattcggagt caatgttaaa cctaggaatt
      421 tacgttcctg gttcgcatcc ttgtagaaac ttaccatcat gagtatgacg acaaaggaga
      481 atgggaatgc acttataatt gcagcacttt gaatcgcatt taaagcttca gcgccgttac
      541 cgccaccagc taataaaagt acaaatgcta ttaaggcctg tgaaattccc caaacaactt
      601 ttaccatact agatggattt aatgaaccaa atgttgtttg cattcctaat acaaatgttg
      661 ctgagtcagc agatgtaata aagaatgatg caattaataa taatgcaatc aacgataaaa
      721 caatgccaaa tggcacatga ttaaacactc caaatagctg tgtttcagga gtcatatcaa
      781 aaatttcttt gtgtttctta cctgtctcga tgcctaatac accaaagaca ctaaaccaaa
      841 caaaactaac aattgctgga actagcaaga caccagaaat gaactctcta attgaacgtc
      901 cttttgaaac tcgtgcaata aacactccaa cgaatggact ccaacttaac caccaacccc
      961 agtaataaag tgtccatgaa gacatccatt cacgtttttg aggatttaaa gctgctgtat
     1021 caaaactatt aaacaagaat gtgtttagta aactacccgt agagctagtt aacatattta
     1081 aaataagaac agttggtcca acaattaaag cagctaccat taaaatagta cctaaaccaa
     1141 tgttcaagtt acttaagtat tgaatacctt tacttaatcc agaccatgca cttgctataa
     1201 ataagatagt aacaacaatg atgataatcg cttgtacaaa cgtattgttt ggaacattga
     1261 acaagtaatg taaaccacca ttaatttgta gagcacccat acctaacgaa acggctaccc
     1321 caacgattgt cgcaaataca gataaaacgt caataaaaat cccaatagga ccttctactt
     1381 tatcacctaa aagaggacgt aaagttctag ataataaacc tggttcacct ttacggaatt
     1441 gcgaatatgc caacgctaac gcaacaacac cataaacagc ccaagcatgg aatccccaat
     1501 ggaaaaatgt tgaacgtaga gcttcagtat aagcttcagt agttttggga tctgctgtag
     1561 gtggcgtagc aaagtgcgcc atcggttcag ctgcaccata aaacaccaaa cctatcccca
     1621 taccagcact aaacaacata gcaaaccatg aaattgtatt aaactcaggt ttgtcatttg
     1681 gtttacctag tttaagtttt ccaataggac taaaaataag gaatatacag aagaacacga
     1741 taatcgtagt aagaataaga taataccaac ctaacttttc tgtaatccac attttaatat
     1801 tattggtaac atagttgaat tgttcaggta aaaatgcacc aagtaatacg actatagcaa
     1861 caacaattgc actatagatg aagactggtg aatacttctt tccatttgga ttctctggtg
     1921 aagaagaatt cataattaat tactcccttc aattctatat ttaattttat gtagtagaat
     1981 aaaaatatta tctaaacatt ttattcaata actcacgcac ctttatagga taacaaaggt
     2041 ctttgcgata atcaaatttt cttgaataat ctattttcta atttttttat ttgttgtata
     2101 attgaggtta catttaaaaa agagaggttg agtgtaatgt taaaagaatt caaagagttc
     2161 gccttaaaag gtaacgtctt agatttagca attgctgttg tgatgggtgc agctttcaac
     2221 aagattatat cttcattagt agaaaatatc attatgccat taattggtaa aattttcgga
     2281 tcagttgatt ttgctaaaga atggtcattc tggggtatta aatacggttt atttatccaa
     2341 tctgttatcg actttattat catcgcgttt gctttattca tctttgttaa gattgcaaat
     2401 acattaatga agaaagaaga agccgaagaa gaagcagttg tggaagaaaa tgttgtgtta
     2461 ttaactgaaa tcagagattt attacgtgag aaaaaataat ttttaaataa aaagagatgg
     2521 gctaaggttg ttctatacat tccaaagtac catctctatt ttttatgttt taatttcttt
     2581 tgaatcttgt tgaactctga tattgatctg atttcacttc taaaactaaa ggtattctat
     2641 ttttcaattc gctcacatgt gaaataatcc caaccattct accagttgat ttaagattta
     2701 ataaagtgtc taacgctgtt tcaagcgttt cttgatctaa tgtaccgaat ccttcatcaa
     2761 taaatattga ttctagtgaa atacctcctg attgctgctg tacaatttcg cttaacccta
     2821 aagctaatgc aagcgacgat tggaaagttt ctccacctga taacgagcta atatgtctag
     2881 acttatttga atgcaaatca aatacatcaa tttctaggcc actaagacca tgagaaaccg
     2941 cttctcgcct aattagttgg tatcgattat ctgacattgt tgctaatctc agatttgctt
     3001 gagcaataat ttgatctaaa tagtaaatta agacaaagtt ttccaatgta agatttttgt
     3061 tattcttacc actgacaatt tcagccaatt gaaatatttc ttgttgatcc tttaattctt
     3121 gatttaagta attaatatgc gaaactatag attgtgtttt attaatatta ttttgacatt
     3181 gataatgaac agcagagtat tcatctatca gtgtattcat ctttcctttt tttagctcat
     3241 attcatcttt taatttatca gagtccaata actctttatc cttagttaat gattctaacc
     3301 tagctatttc catttcaaat tcatgataac gttttttata agtatccctt ttctgctcta
     3361 attcctcttg ctcgcccctc caggctatta tttcatctat atcattgtca tcagttaaat
     3421 tcagcctcga catttccatt tcaatagact gttcattcaa ttctaagtca ttattgtaag
     3481 tttctagttg attttcataa tgatttagat tattttgctc aattaacttt ctttgagaca
     3541 attgcattaa cttatcttca atctcctttg agagttcttg atgctgattc acatcttgaa
     3601 catacttctt ataatccacc tcaaaattag tgatattatt atatttcgag agtgtttcaa
     3661 attcagtgat gagatctcga cataatttca actcagattc atttttattt agacgcaatt
     3721 gtttattatg aatttgcaac gttagattat ctttttcttc tttcatttgt tctataaatt
     3781 tattaagatc tctttgatta ttcaatgcat tttcattttc tagcagacgc ttattcaata
     3841 cttcaagtga aaaatcactt tgcgttttaa tattaatatt cgatattttt tcattaacaa
     3901 atttaatttc agaattatgc acagcaatat tcgattttat agcgtggata tttgcttcaa
     3961 tttctttaat ttcattttga cgtttagcaa tactgtcaaa atcaatatga tgccctaaat
     4021 cctgaatttc attaccacat atcggacatt gatctccaat atttacagca gatttaattt
     4081 cacttataaa gctttcttta tcatttaagt ccaattttgt tttatcgtag cgatctaatt
     4141 ttatttcgag attcttcaat atagatgttt cttcattaat ttgattctct aactcttgct
     4201 tactacctaa tagtttatct aattcagctt tagctttttc attttcttta attatattaa
     4261 tttcattatt tatgttcgtt atcgtattat ttagttcgat aattttctcg taatcacttt
     4321 ggttattacc tattgaatct tcgaccgttt ctaaaccttt aattagatca tttaattcat
     4381 cacctaattg ttcaccctca gttctcaagc gttcaatatt gagataactt tgttgatact
     4441 tattaatatc gttaaataat actctagttt tatcaataaa agagatttta ctttcaatta
     4501 aatctccatt ttcttctaac atttttttct ctttgtctat gtttcgctta tcacaatcaa
     4561 gattcaatat tttattattg gttttagaaa tatctttttt gctattagca attttagttt
     4621 caattttttc tctattttca atataattca ataggtttgt aatatcttgt aacaacttta
     4681 acttagcttc aattccctct atttcaggtt gttgttcttt caattgaacg aaatcaattt
     4741 tatttttgtt gagttgatta atattatctt ttaatatatt attttctaat gttttctttt
     4801 ctattatttc aaattcttta atagcagttt ctttactttc atttacagat gctagtattt
     4861 ctttcgacct agcttgtaaa agtggtatat tttcaatcaa tttgtctatc tgttgagtgg
     4921 caacctctaa taagcctttt atattgtcat catcaaatga ttcaatttct tgccataaaa
     4981 ggtcaatttg ttgatatcta ttctcgattt gagctttttc tttttttact tcttctttta
     5041 atatttctcg tatagcttca aatttttcac tgtcaaacag tgttcttaat atcccttgct
     5101 tttcacgact gtttgatatt aaaaagcgtt tgaattcacc ttgaggcaaa ataaacaatt
     5161 gtcggaattg atctgcattt actcctaata attcaataat gaattgggta cctgaaatta
     5221 ctttactttc tctaatttca tacttgccat ccaccatctc aaatacatca aatttagcgt
     5281 tcgtttttgt tgtattacct tctttgatat aagggccttg tctatgcact ttataaattc
     5341 gatgatttaa ttgaaattca aatgttactg acatcggctg tttaccatca gcgaaatgac
     5401 ttctcaaatc attttcttct ctttgttcag ttgatgcttt accaaacaag gcataagtca
     5461 ttgcatcaaa aatcattgtt ttacccgatc cagtcttacc acttattaaa aacaattcat
     5521 tattatcaat tttagaaaaa tcaatttctt cttttaaaaa ggggccgaaa ttattcaact
     5581 ttaaatgtaa tggtttcatt cattaatcct cctttctcaa ttcgttttct aaaatatttt
     5641 ttatcttttt agattggata tccgataatt ctttatcagt tatatgttta taaaacattt
     5701 cgataattga catgtcatct ttttcactta tttctatagc attattttct tcattgtaat
     5761 taaaagtttc attcgtcagc gctaaagtat taggataaat ttgttttaaa ctcatcattg
     5821 gatcagtaat atgagacata tttttaagtt taaaatgtaa ataattatct ttatttttca
     5881 catgaacttt ttcattaata acatcattat attcgcctga gataatttcc aattgtctaa
     5941 gtggcttaag aggaataaat acatcgttaa taatgccatc attaattgtt acacgtctat
     6001 accctttagc ttgacccgct tccgaaaatg aatactgcaa taaggagcca ctatatttaa
     6061 ttttgtcgtc ttctatacta aatggatgat gcaagtgacc tagcatgaca taatcaaata
     6121 tatcaaaaac acctttctga actgattcaa ccgttccaat agttaatggt ctttcagaat
     6181 cagatgtctt tccaccttga acagtcagat gactaattaa aatattgacg gcatcttcat
     6241 caatttccgg tgctattgtt tcaatacagc gcgtaattcc ctgttgatgt gtttcaatgg
     6301 tgtcatcttc aaagtagtgt ttcatttcgc tcacagtagc ataagggagt gtataaaaat
     6361 taaccccatt tatctctatt ggtgaattaa tcgatgtaaa atctgttctt ataaataact
     6421 gattatgttc aaaccaactc gccccatagt ttaacctctc tttaccat
//
LOCUS       DANRDI010000144         6460 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.145, whole genome shotgun sequence.
ACCESSION   DANRDI010000144 DANRDI010000000
VERSION     DANRDI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.145"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..443)
                     /locus_tag="PZ377_002256"
     CDS             complement(<1..443)
                     /locus_tag="PZ377_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDM6359118.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNV"
     gene            complement(440..652)
                     /locus_tag="PZ377_002257"
     CDS             complement(440..652)
                     /locus_tag="PZ377_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HDM6359119.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            713..1259
                     /locus_tag="PZ377_002258"
                     /pseudo
     CDS             713..1259
                     /locus_tag="PZ377_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1354..2970)
                     /gene="groL"
                     /locus_tag="PZ377_002259"
     CDS             complement(1354..2970)
                     /gene="groL"
                     /locus_tag="PZ377_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDM6359120.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(3046..3330)
                     /gene="groES"
                     /locus_tag="PZ377_002260"
     CDS             complement(3046..3330)
                     /gene="groES"
                     /locus_tag="PZ377_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDM6359121.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            3505..4248
                     /locus_tag="PZ377_002261"
     CDS             3505..4248
                     /locus_tag="PZ377_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDM6359122.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(4273..5538)
                     /locus_tag="PZ377_002262"
     CDS             complement(4273..5538)
                     /locus_tag="PZ377_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359123.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPKPDPDNPKPNPDP
                     KPDPNKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWN
                     PNASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKD
                     YGEVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLAL
                     ITGEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIV
                     CSMMVVFSIMLFLFVKRNKKKNKNESQRR"
     gene            5735..6361
                     /locus_tag="PZ377_002263"
     CDS             5735..6361
                     /locus_tag="PZ377_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDM6359124.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT         1962 a         1177 c          985 g         2336 t
ORIGIN      
        1 acattaccgt ttatatcatg cttatctgta aataatttat gatatttacc caacatatca
       61 cgagctttta aacgatcact aggttttatt ggtacctcta tcagttcaac atgttcatta
      121 tagactaatt gtactttgcc actttgtgga ttttctttat attctccacg cttgactaca
      181 acttctttcg tttctgtttc atcaccgact gccgcattcg ttagcacatg taataactct
      241 tttgcggtta atacattctc atctataatc ttatcttttt gttcttgtat atattgcttg
      301 atgtgcggct ttttcaataa cctacacgct gtcacatgtg cgctatttgc gttatatcct
      361 gcttttatgg cactttgtgt tacattcagt gttctaatat actcattcac aaaacgtgct
      421 tgctttgcag ttaactcact cattttatca cccccacaat tttatctaat atggtttcat
      481 accataatat tacagattgt tctgaacaat ctaaggcact actaatatct tgataactaa
      541 gtccttgtat aagggagtca aaaatataaa actctttatc ggtcgctaat ctgtcaacaa
      601 tcatttctat gtgattcttt ataatatgat cattgacatt atcgtctgtc atcaattcgt
      661 cagaatcttc atcacctatt aaaaagaaat catcagtatt tatttcatca tcgccccgtt
      721 aactagcttt gaagtcttta gcacacttgc atataccggc tgtcgtgctg gcgagatact
      781 agcattgaag tggtctgata ttgattttga aaacaacacg attagtatta ctaaaacata
      841 ttacaatcca aataataaca agaagaaata tcagatactt ccccctaaaa ctgaaagttc
      901 tatcggtaag atttccgttg acccaaatgt aataaaggtg ttgcgtgatt ataagataaa
      961 cgttcaaaat aattggaaaa acgaattata taatgataac cattgtaatg aaaaaactat
     1021 caatgtggat acaaaccatt atgtcacgta ccagcatact caaaaatatc tctacgcact
     1081 cattcagata cacacattgc gcattgctca tagaatcagg tgtacacatt aaagaaatac
     1141 aagaacgatt acggcataaa gatatcaata ccactatgaa tatctatgct aaaatcacaa
     1201 attcatacaa aaaagacgcc tcccataagt ttagtcacca aatggaagac gtctcgtaat
     1261 taaaatatat ttgcgtgcat tctaatttat acttagaatg aatcatactc gtgcataatg
     1321 taattttcta gttagtcaaa actataaaca gttttacatc attcctggca tgccacccat
     1381 gttaggttgg tcattatttt tttctggaat tgatgctaca accgcttcag tcgttaagaa
     1441 cattgctgca acacttgcag catgttgtaa tgctgagcgt gttactttag ttggatcaac
     1501 gataccttct tctaacatat taacccactc gtttgtagca gcgttaaaac caacacccgg
     1561 ctctgcgttt ttcaaacgtt ctacaataac agaaccttct aatcctgcat tttcagcaat
     1621 ttgacgaact ggtgcagtta atgctttaag tacaatattt acacctgttt caatgtcacc
     1681 ttcagcttca atttcactta ctttttggta aacatttact aatgcagtac caccacctgc
     1741 aacaatacct tcttcaactg ctgcacgtgt agaatttaat gcatcttcaa tacgtaattt
     1801 acgttcttta agctctgttt cacttgctgc acctactttg ataactgcaa caccacctgc
     1861 taatttagct aagcgctctt gtaatttttc acgatcaaag tcagattcag tttcttcaat
     1921 ttgagatttc aattggctaa cacgtgcatc aatgctgttt tcgtcaccgt caccatcaac
     1981 aacagtggta ttatctttag ttacttctac tttacttgca gtacctaaca tatcaattga
     2041 tgcatctttt aaatctaagc ctaaatcatc agtaatcact tgcgcaccag ttaaaatagc
     2101 taaatcttca agcatcgctt ttctacgatc accaaaacca ggtgctttta ctgcaacagc
     2161 tgtaaatgtg ccacgcatac ggtttagcac gatatttgtt aatgcatcgc cttcaacttc
     2221 atcagctaca attaagattg gacgattaga ttgaaccact tgttctaata aaggtaagat
     2281 atcttggaaa gacgagattt tcttatctgt tactaaaatg tatgggcgtt ctaattcagc
     2341 aaccatttta tctgaatcag taaccatata cggtgattga taaccacgat caaattgcat
     2401 accttcaacc acttctagtt cagtgtttag tccatttgat tcttcaattg taatgacacc
     2461 atcgttacct actttttcca tagcttcaga aatataacgt ccaatttctt catctgctgc
     2521 tgaaatcgca cctacttgcg caatttcatt tttattttca actttttgag aattttcatg
     2581 taacgcttca acagcaactt taactgcttt gtcgatacct tgtcgtaaac caactgggtt
     2641 cgcaccactt gtaacatttt tcaagccttc ttgaatcatt gcttgagcta atactgttgc
     2701 agttgtcgta ccgtcaccag caatttcatt tgtcttattt gcgacttctt gaactagttt
     2761 agcccccata ttttcatatg gatcttctaa ttcgatttct ttagcaatcg tcacaccatc
     2821 attcgtaatt aaaggtgctg taaactcttt atctaataca acattacgtc ctttaggacc
     2881 aatcgttact ttaactgcat ttgcaagttg gtcaacacca cgtaacattg cttgacgtgc
     2941 atcttcagag aatttcaatt gtttaaccat aaatgataaa cctccatttg tttagttgtg
     3001 tttcattttc gttctattta atttatttat gaattaagtt ctgtattatt caataactgc
     3061 taaaatatct tcttcattta ataccagata tgtttcatta tctcgtttaa cttctgtacc
     3121 agcatattgt tggaacacga cacggtcccc ttctttcact tcaggagtca ctcttgtacc
     3181 atcatttaat aggcgtccag ttcctactgc aacgataacg ccttcgtttg atttttcttt
     3241 agcactatca gttaaaacaa taccactttt agttgtttgt tcttgttctt ttttctcaat
     3301 aatcacacga tttccaattg gttttagcat gattgttcct ccttaaaaaa cctaaagttt
     3361 agcacttaac attaaagagt gctaacatac atttataata atcaaatttg gtcaaaattt
     3421 caagtcataa ccttttaatt aagttttgta acttcaagtt atttacgata aaataaatta
     3481 taaacaaata tttggaggaa aattatgaca agattatggg catcattgct aactgttatt
     3541 atttatatat tgtctcaatt tttaccgctt ctcattgtaa aaaaattacc atttgtacaa
     3601 tatagtggca tagaactgac taaagcagtc atttacatac aacttgttct atttttaatc
     3661 gccgccacga cgattatttt aattaattta aaaattaaaa atccaacaaa attagaatta
     3721 gaagttaaag aacctaaaaa atatatcatt ccatgggcat tgcttggatt tgcattggta
     3781 atgatttatc aaatggtagt gagcattgta ttaacgcaaa tttatggtgg acaacaagta
     3841 agtcctaata cagaaaagct aattattatt gctcgaaaaa tacctatatt tatcttcttt
     3901 gtatctatta ttggtccttt attagaagaa tatgtattca gaaaagtaat ctttggagaa
     3961 ttatttaatg cgattaaagg taatcgtatc gtggcattta ttattgctac aacagtaagt
     4021 tcattaatat ttgcattagc acataatgat ttcaaattta ttccagttta ttttggtatg
     4081 ggtgtcattt tttcattagc atatgtttgg acaaaacggc ttgctgttcc aattattatc
     4141 catatgttac aaaacggatt tgtcgttata ttccaattac ttaatcctga ggctttgaaa
     4201 aaagccacgg aacaagcgaa ttttatatat cacattttta ttccataata aaaaaaacga
     4261 atcacacacg gattatcgtc gctgtgattc gtttttattt ttctttttat ttcgctttac
     4321 aaataagaat agcatgattg aaaataccac catcatagaa catacaattg ctagccaact
     4381 tttactatat gcagttgacg cacttttctt attatcttct tgttttttaa ctactgtaaa
     4441 atcgtctttt tcatgcggtt caaatgagcg ttgttttgaa tccggcttat cttcgggctt
     4501 tttcaaatct ggcattgatc caatttcacc agtaattaat gcgagtactt gttcatctaa
     4561 attacgatag tacctttctg atttcaatgg attgaaatat tggaatctaa agtaattcga
     4621 ttgctgttgt aatccattta aatatgcatt tccgctgaaa ttttgttttc gaataatatt
     4681 ataaatgtct tcatcagtaa cttctccata atctttgccc aacttattaa tttgatttaa
     4741 aatatacgga ttatacttgt aagccccatt tgccaaggct aaaaatcgtt gggatacaaa
     4801 atcattacca gtaggatttt gtgagtttcc ttgattccca tttggttgcc attgaccttg
     4861 attagatcca tctgaagcat ttgggttcca tgtcccccca tttttcgagc caccagaatg
     4921 attggaatcc ccaggttgat cgggatctgg tgacgggtta ggatttggct tattagggtc
     4981 tggttttgga ttcggatttg gctttggttt atctggatct ggttttggat ccggatttgg
     5041 cttattaggg tctggttttg gatctggatt tggtttcggg ttatctggat ctggttttgg
     5101 atctggtttt ggatccggat ttggctttgg tttatctggg tctggttttg gatccggttt
     5161 tggtttcggg ttatctggat ctggtttagg attcggactt gggttttggt tatttgggtc
     5221 tggttttgaa tctgtacttg ggttttggtt gtttgagtct ggcttcacat tattcgaatc
     5281 aggattagca tcacgactat tcgaacctgt tccttcatta cccgtttgat ttgattcgtg
     5341 aggtgtccca gtctcattat tattcatttc actatttact gatggtgaag ttatattatg
     5401 attcaaatta gtagttggtt tttctccatt taagttatcc tttgcttcaa ctgtgtgcgt
     5461 tgcaagccct aaactactac tacttaaaat taaaatactt gttgaaagta acattttctt
     5521 aaaccaatga tatgacattt cacgagacac ctcccttttt catgtaagta ttaatctaag
     5581 tataataaat atgcgttgaa aatgcattta tatttggaat ttgaaaatga atattaatat
     5641 tcttgaaatt ttcgtaaata ttgctttaca agtacgagta taaattttat acttagtcta
     5701 ttaagtattc tttactaaag aaggagcaat aattatgggc ttatttaaaa aagataaaaa
     5761 agcaatgact tttgacaatg caatggaaga aagacgttca atatataatt tgaaagattc
     5821 gatatcaatt agcgatgacg aattagaatc aatgattgca catgcagtta aacatgttcc
     5881 ttcatcattt aattctcaat caactagaat tgttctcttg ttaaatgaca acaataataa
     5941 attttgggat aacacaaaag caattttaaa agaagtcatg ggagagaatc gtgatttcga
     6001 gccaactgaa caaaaaattg ataacttcaa acattcatac ggtacaattt tgttttatga
     6061 agatcaagat gttgtaagtg gtttacaaga acaaatgcca aactactatg ataattttgc
     6121 aatatggtca actcaaacaa atgccatgca tcaatttgca atttggactg cattagctac
     6181 taaaggcata ggtgcttcat tacaacacta taatccactt gttgatgaga tgacttcaaa
     6241 tgaatttaat attcctaaaa gttggaaact tattgctcaa atgccatttg gtgatattcg
     6301 tgaagctgct ggcgaaaaaa catttaatcc agtggaagat cgtttcgtaa taaaaaaata
     6361 atcaatatat tgttaggaat gggacagaaa tgataaagaa tcattagtta ttcattatgt
     6421 agtagttctt acacattagc cgctgctaat gtgcacttaa
//
LOCUS       DANRDI010000145         6434 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.146, whole genome shotgun sequence.
ACCESSION   DANRDI010000145 DANRDI010000000
VERSION     DANRDI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.146"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(17..1378)
                     /locus_tag="PZ377_002264"
     CDS             complement(17..1378)
                     /locus_tag="PZ377_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6359125.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1726..2847
                     /locus_tag="PZ377_002265"
     CDS             1726..2847
                     /locus_tag="PZ377_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDM6359126.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2896..4164
                     /gene="icd"
                     /locus_tag="PZ377_002266"
     CDS             2896..4164
                     /gene="icd"
                     /locus_tag="PZ377_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDM6359127.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4554..5258
                     /locus_tag="PZ377_002267"
     CDS             4554..5258
                     /locus_tag="PZ377_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDM6359128.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5258..>6434
                     /locus_tag="PZ377_002268"
     CDS             5258..>6434
                     /locus_tag="PZ377_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDM6359129.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     "
BASE COUNT         2316 a          940 c         1201 g         1977 t
ORIGIN      
        1 gccctaaaac ttgttgttat ttatttgatt tagcagcgat acgtttatat cttaagtaca
       61 taaatgctaa aagtataaac caaatcggaa taaaataaat tgcacgtctt gtatcaacat
      121 taataaataa taacccgaac acaaaaatga agaatacaaa tattaagtag cccatatatt
      181 tgccacctaa tagtttgtac gtagcatttt tatgtagatc tgggttttta cgactataat
      241 tgatatatgc aatgataatc agaccccata caactaaaaa taacactgta gagatggtag
      301 tcacatacgt aaatactttt gtcgcatctg ggaaaatata gtttagtaat gctgccacaa
      361 gtaataatgc tgatgaagca aagattgcaa catgtggaac gccatattta ttcgtcttag
      421 aaaagttcgg aggtgcttgt tgttgacttg ataaaccgaa aagcatacgg ctatttgaga
      481 atataccact gttacatgat gaagcagcag cggttaatac tacaaaatta atcaagcccg
      541 cagcaaacgg aattccgatc aatgcgaata attttacgaa tggactgtta tcaggatcaa
      601 cttgctgcca agggataata gacatgataa ccgctaacgc cccaacgtaa aatattaaaa
      661 ttctaatggg tacactgtta attgcttttg gaattgtttt aactggatct tttgtttccc
      721 cagctgtaac accaatcatt tcaattccta caaatgagaa tagtgccatt tggaaagaca
      781 taaagaatcc agaagcacct ttagcgaata tgccgtgttc atatagattt gtgaaactgg
      841 catgcccgaa ttgagtttta aatgcaaata gaatcatgac gaaaccaact actattaaac
      901 caataattgt cgctatttta atgatagaga accaaaattc taattctccg aaaagtcttg
      961 cgctaagtag gttgaatgac attaataaca gtacacaaaa tagtgcactt atccagtttg
     1021 gaatttctgg gaaccaaaag ctaacatatt ttgccacagc cgttacttca gccatacctg
     1081 taataatcca acagaaccag tatgtccatc cggtaacaaa tcctgcaaaa ggcccaatat
     1141 atgtattggt tacatctgcg aaagatttaa attcagtatt ctgtataatg atttctccta
     1201 aacctcgcat aaacataaat aacataaatc ctataatgat gtatgttaat agaattgaag
     1261 ggccggttaa tgcaatcgtt tgaccagcac ctaagaataa gcctgtacca attgcaccgc
     1321 caattgcaat taattgtatg tggcgattgc tcagttccct ttgtaatttt tcagccataa
     1381 tacatctccc ttaaatatag atatgtttat tatgcactta tattgagata tatacaatta
     1441 ttttcggtaa aaatgtgtaa aattccatgt taatatactt tggtttttat aatcatatat
     1501 aataaccaat tgaaaattta attctattgt aaaattcatg gattattcac atcttgaaaa
     1561 agctttaatg gtgctatttg tggctattct gtgacattta catagattta caaaaaaatt
     1621 gttgcacata taatgccagt ttttatattt cacaaacgaa atgcgtttag ctataatatt
     1681 agttgaaagc catttcataa agaaacagta aaggggaaat ttatcatggc agaattacaa
     1741 agaggtttag aaggggttat cgcagcggag actaaaataa gttcaattat tgaaagtcaa
     1801 ttgacttatg ccggctatga tattgatgat ctagctgaaa atgcgcaatt tgaagaagtt
     1861 attttcctat tatggaacta tagattgcca aacgaagaag agcttgctca tctaaaaggg
     1921 aaattaaatc aatacatgac attaaatcct cgtgtgtata cacattttga ggagtatgtt
     1981 acagatcacg tgcatccaat gacagcatta cgtacgtcat tatcatatat tgcacatttc
     2041 gatcctgatg ctgaaaatga atcagatgaa aatcgttatg aaagagcaat gcgtatacag
     2101 gctaaagtag catcattagt tacagcgttt gctcgagtaa gacaagataa agaaccactt
     2161 aagcctaatc ctgacttaag ttatgcggca aacttcctat atatgttacg tggggaatta
     2221 ccaacagata tagaagtaga agccttcaat aaagcactta ttttacacgc tgatcatgag
     2281 ttgaacgcat ctgcatttac ggcacgttgt gcggtatcat cattgtcaga tatgtactca
     2341 ggtattgtag cagccgtagg ttctctgaaa gggccattac atggtggtgc aaacgaacaa
     2401 gttatgacga tgttatctga gattgggtca attgaaaatg ttgatgctta cttagatgaa
     2461 aaatttgcta ataaagataa agtaatgggc ttcggtcatc gtgtatataa agatggtgat
     2521 cctagagcga aatatttaag agaaatgagc cgtcaaatta cgaaagacgc tggtcgtgaa
     2581 gaattatttg aaatgtcagt gaaaatggaa aaacgtatgg cagaagaaaa aggattaatt
     2641 cctaatgttg atttttatag tgcgagtgtt tatcactgta tggaaatacc tcatgactta
     2701 ttcacgccaa tctttgctgt aagtcgttct gcaggatgga ttgctcatat tttagaacaa
     2761 tataaagata atagaattat gcgtcctaga gcgaaatata ttggcgaaac gaatcgtaag
     2821 tatatcccgc ttgaagaaag aaaataatca atacaaatta aaaatgaaga tgtaaaattt
     2881 ggaggtaaaa taactatgac tgcagaaaaa attactcaag gaactgaagg attaaacgta
     2941 cctaatgaac caattatccc atttattatc ggtgatggaa ttggaccgga tatttggaag
     3001 gcagcaagcc gagttataga tgctgctgtt gagaaagcct ataatggcga aaaacgcatt
     3061 gaatggaaag aagtgctagc tggccaaaaa gcatttgata caactggtga atggttacct
     3121 caagaaacac ttgatacaat taaagaatat ttaattgctg ttaaaggacc tttaacaaca
     3181 ccaattggtg gtggtattag atcattaaat gtggctttac gccaagaatt agatttattt
     3241 acttgcttaa gaccggtacg ttggtttaaa ggagtaccat cacctgttaa acgtccacaa
     3301 gatgttgata tggttatttt ccgtgaaaat actgaagaca tttatgctgg tattgaattt
     3361 aaagaaggta caacagaagt taaaaaggta attgacttct tacaaaacga aatgggtgcg
     3421 acaaacattc gattcccaga aacttcaggt attggtatta aaccagtttc taaagaagga
     3481 actgagcgat tagttagagc agctatacaa tatgctatcg ataataaccg taaatcagtt
     3541 actttagttc ataaaggtaa tattatgaaa tttacagaag gctcatttaa gcagtggggt
     3601 tacgatttag cattatctga atttggtgat caagtattca cttggcaaca atatgacgaa
     3661 attgttgaaa atgaaggcag agatgctgct aatgctgctc aagaaaaagc tgaaaaagaa
     3721 ggcaagatta tcattaaaga ttctattgct gacattttct tacaacaaat tttaactcgt
     3781 ccagctgagc atgatgttgt agcaactatg aacttgaatg gtgactatat ttcagatgct
     3841 ttagctgcac aagttggtgg tattggtatt gcgccaggtg caaacattaa ttatgaaaca
     3901 ggtcatgcta tttttgaagc aacacatggt acagctccaa aatatgcagg tttaaataaa
     3961 gtgaatccat cttcagtaat tttaagttct gtattaatgt tagaacattt aggatggcaa
     4021 gaagcggcag ataagattac agattcaatt gaagatacaa ttgcttcaaa agttgttact
     4081 tatgactttg cccgtttaat ggatggtgct gaagaagttt ctacatcagc atttgcagat
     4141 gaattgatta aaaatttaaa ataagcaaaa taaaattagg tgagtctagc aactttaatt
     4201 gctaggttcg cctttttaaa tgaattgaag ttggcaacag gatagaaata ataaagagtc
     4261 accaatgatt gattatgtag tgtttcttac acattagaca aagcgaatat gtacataagc
     4321 ttaggaataa atgagtaaaa ctcatgcata ggtagtggga tagaaatgct atttttacaa
     4381 aatttatttc gttgtcccag agtaagtcat gcaaatgata gaaggaaata taagaagtat
     4441 tttatttata tgtaaagtaa tgtaaaaaat gtgtaaagat aatgtgtagg atgttatttt
     4501 tataaatagt tcggttataa tgtaaatata attataagtt agggaggcat accatgtcgc
     4561 aaaaagtgtt ggtagtagat gacgaacatt caatcgtaac attacttaaa tataacttag
     4621 aaacagctgg ttatgaagtt gttgtcgcat ttgatggtga tgaggcttta gaaaaggtag
     4681 aaagtgaaca gccagattta attattttag atgttatgct acctaaaaaa gatggcattg
     4741 acgtatgtaa gactgtaaga acaaataaaa atttagtccc tatattaatg ttaacggcga
     4801 aaaatgatga atttgatcgg gtattaggtt tagaattagg tgccgatgac tatatgacta
     4861 agcctttttc acctagagaa gttgttgccc gtgtgaaagc cattttaaga cgttctcaat
     4921 ttgtaaaaga agtagaagag attgacgatg atattaccat cggatcgata cgtattagac
     4981 ccgattattt tgaagtatat aagcataatg agttattgga gctaacaccg aaagaatttg
     5041 aactcttgct ttatttgata gaaagacaag gcagagtcat tacgagagag catatgttaa
     5101 atacggtatg gaattatgaa tttgcaggag actcaagaat tgttgatgta catatcagtc
     5161 atttaagaga taaattagaa gataatccta agaagccaca acttattaaa actgtgcgtg
     5221 gcttaggcta taaattggaa agacctaaag aacaatgatg aagtttcacc accgcttaat
     5281 gttattgata agcagtatat taattatcag ttttgttaca ttaggcatta ttattcacgc
     5341 ttccatcaca tatgttttat cagaaaaaca cgaacaagct ctagcaacag atgcaagatc
     5401 atatatttat ttagtccaag ataatgagat tgataaagtt aaaaaaattg taaaacagca
     5461 aaatatagat ttattgataa ctaagcatga taaaaaggtg tttagtagtg gcaaactgaa
     5521 agctatccca aatagtgata gctatattca aaaaagggac gtttttattt ttaataagaa
     5581 ggttaacggt tatcatgtat ggattaaagg ttacaataac gatattacag aaatgcattg
     5641 gacgttgtgg aaatatctta ttttgacatg tttagttgta ctcatttgtc tgtattttgc
     5701 aagccgtagt tttaagcgta cattaatccg tcccattcaa gaagttactt atgcaacaca
     5761 attgttggca aatggttatt atcacatacg tgtacctgaa agtaatgttg tagaaactaa
     5821 agcgcttttt gtgtctacca atgatttagc acgacgcctt caaaagctta ataatgagca
     5881 aaaaattcaa tccaaccgtt taaaaacaac gattgaaaat attccaagtg ctattttaat
     5941 gatagatcga aatggaaaaa tcgttgttgc aaataaagcg tattatgaac agttcaacat
     6001 ctcgcataat attgaacaag taggctatca cggctatgtt aatactgaaa ttgagcaatt
     6061 aattttagaa agttttaaag ttgaaaaacc tatttatgaa caattggaag ttgcaataaa
     6121 ccaagtccat gccaagtact ttgatatttc atgcgttccg attttaacta ggtctcaaaa
     6181 aagtttgcaa gggattttag ttgtgatgca tgatataaca aatctgaaac aacttgaaaa
     6241 tctaagacgt gaatttgtag caaatgtttc acatgaatta aaaacaccta ttacttcaat
     6301 taaggggttt gctgaaacgc ttattgatgg tgcaaaaaat gatgcggaat cattagacat
     6361 gtttttaaac ataattttaa aagaatcaaa tcgaatagaa tctttagtaa cagatctgtt
     6421 agatttatcg cata
//
LOCUS       DANRDI010000146         6309 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.147, whole genome shotgun sequence.
ACCESSION   DANRDI010000146 DANRDI010000000
VERSION     DANRDI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.147"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..632
                     /gene="hlgB"
                     /locus_tag="PZ377_002269"
     CDS             <1..632
                     /gene="hlgB"
                     /locus_tag="PZ377_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HDM6359130.1"
                     /translation="NDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(684..1151)
                     /locus_tag="PZ377_002270"
     CDS             complement(684..1151)
                     /locus_tag="PZ377_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDM6359131.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(1162..1854)
                     /locus_tag="PZ377_002271"
     CDS             complement(1162..1854)
                     /locus_tag="PZ377_002271"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDM6359132.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(1865..2980)
                     /locus_tag="PZ377_002272"
     CDS             complement(1865..2980)
                     /locus_tag="PZ377_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDM6359133.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(2958..3965)
                     /gene="bioB"
                     /locus_tag="PZ377_002273"
     CDS             complement(2958..3965)
                     /gene="bioB"
                     /locus_tag="PZ377_002273"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDM6359134.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            complement(3967..5325)
                     /gene="bioA"
                     /locus_tag="PZ377_002274"
     CDS             complement(3967..5325)
                     /gene="bioA"
                     /locus_tag="PZ377_002274"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDM6359135.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(5303..5989)
                     /gene="bioD"
                     /locus_tag="PZ377_002275"
     CDS             complement(5303..5989)
                     /gene="bioD"
                     /locus_tag="PZ377_002275"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDM6359136.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(6043..6210)
                     /locus_tag="PZ377_002276"
     CDS             complement(6043..6210)
                     /locus_tag="PZ377_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359137.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
BASE COUNT         2049 a         1182 c          854 g         2224 t
ORIGIN      
        1 ctaatgattc agtaaacgtc gttgattatg caccaaaaaa tcaaaatgaa gagtttcaag
       61 ttcaaaatac tttaggctat acatttggtg gtgacattag tatctctaat ggtttatctg
      121 gtggacttaa tggaaataca gctttttctg aaacaattaa ttataaacaa gaaagttaca
      181 gaacaacatt aagtcgcaac acaaattata aaaatgttgg ctggggagtt gaagcacata
      241 aaattatgaa taatggttgg ggaccttatg gaagagatag cttccaccca acatatggta
      301 atgaactctt cttagctggc agacaaagca gtgcatacgc tggccaaaac ttcatagcgc
      361 aacaccaaat gccattatta tctagaagta acttcaatcc agaattttta agcgtactat
      421 cacacagaca agatggcgct aaaaaatcta aaattacagt aacttatcaa cgtgaaatgg
      481 atttatacca aattcgttgg aatggcttct actgggcagg cgcaaattat aaaaacttta
      541 aaactagaac atttaaatca acatatgaaa ttgattggga aaatcacaaa gtgaaattgt
      601 tagatacaaa agaaactgaa aacaataaat agctagtaaa acacggtcgc caacagtaat
      661 tgtgacgacc gtgttttgat ttattatctt agtaatactg ccattctttt tctcaatgtg
      721 agatataaag gaatagctac aattaaagtg aatattacgc ctggaatcgc gtttaacaac
      781 actacccaca caggtaaatt taaaataata gataatagta gcctagatac caaactgcct
      841 aatacacttg ctaaaactaa tgatagtaca tttattttca ataaataaac aactgcaata
      901 gctataactc taaatataat agagataatc acattaattg gattaaatac gccaaatact
      961 agtaataata ggctagataa taatccacct aaaaagtact ttttaattcc aaagaaagct
     1021 aatatcaata atgctgccgg tgcagataat tgaaaatcta atcctggtat aatggacggt
     1081 attttcaaaa ctgccaaaat ggttaaaatc gcagcaatga cactaatttg agtaatatct
     1141 tttgatgtca tactaaaacc cctataccgt ttcataaaca acttgcttcg gtgtgctttc
     1201 taaaaatgat atgtaatgat ttaaatcaat acaatcgtcc acaaatatta ttctgcctcc
     1261 atatctcgta ttaactggtt taatatcaaa taatcgatgg taaccaattt tagcagcggc
     1321 aaaataacct gtcgtatacg ttaagtcatc ggacacgcaa agttctcctt tgacatacgg
     1381 atgcgcattg atacaactag caattgctaa ggcatcagtc actctttcat taagatcacc
     1441 tttattattt atatcttcaa acgaaaaatg tgttgcccta atcccccttt gtccaaatga
     1501 atctaaacgt ttaccagata tagcagatag aataatagct cctgtataaa ccgtttcatt
     1561 tttaatatat gtcatccctt gatttagcgc ttgttcagtg acaccacatt cttgtgttaa
     1621 atgttggaga tttgctttat catcctcaat aatttgtaat gcttttattt gttgaatcgg
     1681 ttccatgatt ttttgtattt ttatattgag aaaatccggt tgcccatttt catgaaaaaa
     1741 acctttatta taaaaccttt gtaccgtctg ttctattttg tcaaactcac aaatcgtttc
     1801 agcaccacta atatgaacat cttgattgct agaacgcatt tttatactat acatgacgat
     1861 cacctcaatc ttcttgatgc aaaatttcaa acaacctatc tatatcttgt tcagtatgaa
     1921 aatacgacaa tgatattctt aacattggct tagtcacagt tggatacctt aaataacttg
     1981 taaacacatg atgctttaat aatgtttgat gaatgttctc agccgcttct atgtcatcaa
     2041 actcaataaa cttaatcggc gagtttgaac tattataatt aacattgagt gcttttaact
     2101 tttggttaaa atatttactc aaactattta atttagtgcg tctatcatca gcatttatta
     2161 acttttcaat gtttcttttt ataaaataca aattataaat tggcaaacta cttgagtaga
     2221 tgagtggtct accgtgatta attaacatat ccttcacatc atttgaactt aaaatcacac
     2281 ccccgtatgc accacatgct ttagataaac tagaagtgag tatatctaca ccttgataat
     2341 tcgagtaatt ctctattcca aaactatgtg aaacatcgag tatcagtgtt gcgttatatt
     2401 tatgctttaa tgagactaat tgaccaatat ccacaacgtc gccattcgtt gaaaatacac
     2461 tatcagatat gattattttt ggtatatttt gattagggta tttctctaac cttttttcta
     2521 aatcagcaat atctaaatgc ttatatatca ctttttctaa accacttaac ttaataccgt
     2581 caataatact cgcatgattt tcttgatctg aaaacacgac acaatttgta tttttgaaaa
     2641 tattaaataa cgccaaatta gcatcataac cactatttaa gatagtacat gcactatatc
     2701 cgagccaacc tgctaacatt gtttcaattt cttcataagc tgtcgaactt ccactaatta
     2761 atcttgaact tgataagtga taactatact tccgcataaa tctttcgaaa tcatccttat
     2821 caaacgctat ttgacctaat cctaaataat cattagatgt atagttcgta catctcttat
     2881 tttctacttc aatatactgt ctatctatat accctaccga tttaagcgac cgatataacc
     2941 ctttctgttg taataaatca atttgctctt gaaacttcat tcttgttttc cttattttca
     3001 caagtgtcat aatcaatttc aaagcctaaa tcattaatca tatcgtagtc taattggttc
     3061 ggttgcccac cagtaattag ataatcaccg acaaatattg aattcgccgc ttttaatgct
     3121 aatggctgta acgaacgtaa gttgacctct cttcctccag caatacgaat ttctttcgta
     3181 ggattgatta atcggaataa tgctacgatt cttaaacatt tcattggtgt taaatcatcc
     3241 atgcttccaa actttgtgcc tttgattgga tgcaaaaaat taatcggaat actgtcggca
     3301 tccatttctt ttaaagcaaa tgccatatca acaatatctt gattagattc tcccatacca
     3361 caaatcacgc cagaacatgg tgatatatta ttcgctttca ttagttctat cgtatctgtt
     3421 ctatctttat aactatgcgt tgtcacgacg ttatcatggt aattttcact tgtattaata
     3481 ttgtggttat atctgtctac accagctgac ttaagtttct tagcttgttc gtcattcgtt
     3541 aatcctaaac atgcacagat ttttagttgc gggtgttgag atttaatcgt tcttacagta
     3601 ttactaatat gatcaacttc tttatcgctc ggtcctctac cactcataac aatacaatat
     3661 gttccaatat gattatcatg tgccaccttt gctccatcga taatttgttc ctctggaatt
     3721 aaagcatatc gctgtttttg tttaatatct cgtgattgtc cacagtaccc acaattttca
     3781 ggacatatac cacttttagc atttaaaatc atgtttaatt ttactttttt accaaaataa
     3841 tgttttctta aaatgtacgc ctcatttaat aaatctaagg tatcaatatt agtatcctca
     3901 taaattttca atacagtctc ttttgttaat tgttcccctt gtaatatgcg tttagccaaa
     3961 ttcatattaa cacttcctat ctaaaatgtt atgtactgcc tttttaaaaa tacgtaccat
     4021 cgttttcact tctttatttg acatacttaa cactggcaca aacgtaatga cattttctaa
     4081 atttctaatc attagcccgt tttctttaca attacgtaca atacgttcaa ctttttcaat
     4141 ttctaacggc gttttactat ctttatctgt aactaattca actccaaaca ttaagcctcg
     4201 tcctcttaca tcaccaacat ttcgatgacg cttcagcgca tgtaactgtt tctcaagtgt
     4261 tgatgatgtc gtttcaatat gtgacaataa cttacgtttt tcataaagtc ttatattttc
     4321 taatgcaacc gtacaaacga tttgatttcc ggtgtatgta tgaccatgga aaaaggtatt
     4381 cacaccatgc gaatcactta aaaatgcatt gtatattttt ttagatgtca atgtagctgc
     4441 aagtggtaag tagccaccag taatcgcctt acctaaacac ataatatccg gttgaacatc
     4501 ttcatgattg catgcaaaca tctttccagt tctcccaaaa ccaactgcta cctcatcaca
     4561 aattaataag acatcgtatt ttttgcacaa tttctcgact tctttcaaaa agcctttagg
     4621 atgaacaaac aagcctgtcg caccttgaat caacggttcc aatataaacc ctgcgatttc
     4681 atcatttctt tcaattagaa tttgctctat attcgttaaa atagctgtca tcatttcatt
     4741 ttcagtatcg taattactgc gatagaaaga tgggctttct acttgaatat tctcaaatat
     4801 taagtcttta aatattttat gaaaggtctt gataccacca acacttaccg caccaatcgt
     4861 atccccatga taaccgtgat ttagcgttat aaacttgttt ttcttggcat atttttctct
     4921 atcaatattt ttccaatact gatatgccat ctttattgcg atttcaacag acgcactgcc
     4981 tgtatcagaa taaaatactt ttcttagatt acttggcgtg atttcgatta atttttccgc
     5041 aagttctatt gacggaatat ttgatgatcc tagcagcgta gaatgggcaa ttttattgag
     5101 ttgcttttta attaccttat ttaagtattt gttattatga ccatgcacat tgacccacaa
     5161 cgatgcataa ccatctaaat atttattgcc attcgtatcg taaaggtaac taccctttcc
     5221 tttttcaatg atgattgctt cttctttgct atatacaccc atttgtgtaa atggatgcca
     5281 aacatattct gagtcttttt gtttaagttg ttgtgtgtaa ttcattttta actcctatta
     5341 attgttttaa aaatgcttct gagaaatttt catacgtggc atgttcttca aatgtataga
     5401 ctgttttatt tgttaatttt ccaatcgtca tttgattgtc tttttcaata tagctgtctg
     5461 tatagcgatt cattattaaa aaattactcg ccgatacatt ctgattaaca taatcttggt
     5521 gaacaatggc atcgctaata gcacctaatt ttgatggcaa cacactgatg acacaatctg
     5581 cacaatcatt gattagatct ttagtcatgt agaaatcatc tgtaccttca tatattggta
     5641 cggcaattcc cccagcaccc tcaattaaga taaaatcaaa ttccttatct aaaactttta
     5701 ccttatctaa tacacgctgc ttatttagaa aaatttgatc tgtcatttta aatgcaaggt
     5761 gtggtgatac aggttgctta aaagtataaa gtgacgttat gtcatagctt aaatcacatt
     5821 catttttaaa tacttctaaa tctggaaacg tcccgtcttg gcgttcctca gtttgaaatg
     5881 gtttaaaaat acaaactcga tgaccacgtg ttttcaaagc atggtataaa tgctttgtaa
     5941 cataggtttt gcctacatca gtattcgtac ttgtaataaa aatcctcata aaaataacca
     6001 cctcaatcca aatgtaaact tttataatta ataagtttac atttaaggcg catgtttttc
     6061 aagaaaacat gaatttaaac ttctcagtat aactataaat tttggcaaaa ttttgacctt
     6121 gctattgaaa tgactaagac ttcttaagga attcgggcag atataaacag cgattcgata
     6181 aaatatgtca gttaaaatta ataattgcat tatttcttaa taaaaagaca tcctagcttt
     6241 accatcttgt gaaacaatcc attaattgta atattacgtt gagcgccttg ttcatcaatg
     6301 ctataatta
//
LOCUS       DANRDI010000147         6305 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.148, whole genome shotgun sequence.
ACCESSION   DANRDI010000147 DANRDI010000000
VERSION     DANRDI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.148"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..136)
                     /locus_tag="PZ377_002277"
     CDS             complement(<1..136)
                     /locus_tag="PZ377_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDM6359138.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     Y"
     gene            440..1090
                     /locus_tag="PZ377_002278"
     CDS             440..1090
                     /locus_tag="PZ377_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDM6359139.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            1171..2166
                     /locus_tag="PZ377_002279"
     CDS             1171..2166
                     /locus_tag="PZ377_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDM6359140.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            2242..2868
                     /locus_tag="PZ377_002280"
                     /pseudo
     CDS             2242..2868
                     /locus_tag="PZ377_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2909..3253
                     /locus_tag="PZ377_002281"
     CDS             2909..3253
                     /locus_tag="PZ377_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDM6359141.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            3351..3902
                     /locus_tag="PZ377_002282"
     CDS             3351..3902
                     /locus_tag="PZ377_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359142.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
     gene            complement(4120..4761)
                     /locus_tag="PZ377_002283"
     CDS             complement(4120..4761)
                     /locus_tag="PZ377_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDM6359143.1"
                     /translation="MFTPIVINLLNDVGSIFLVRVGNQLKVIEKVHYEDILESKIVRL
                     FQAFIKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYLSGRTIYINNNH
                     SINNTRFSIARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQFSVDLLMPKKQI
                     EALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYNLGIHI"
     gene            complement(4875..5060)
                     /locus_tag="PZ377_002284"
     CDS             complement(4875..5060)
                     /locus_tag="PZ377_002284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359144.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
     gene            complement(5062..5238)
                     /locus_tag="PZ377_002285"
     CDS             complement(5062..5238)
                     /locus_tag="PZ377_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359145.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            complement(5249..5632)
                     /locus_tag="PZ377_002286"
     CDS             complement(5249..5632)
                     /locus_tag="PZ377_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359146.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
BASE COUNT         2362 a         1016 c          913 g         2014 t
ORIGIN      
        1 catagtctgc tgaaaagtaa atactaccta ttgtttcatt atgttgttgt atttcaaatc
       61 gttggcctac tattttatta tttgtgctac aactacttaa aaaaatcagt agatatagca
      121 ttaaacatat tttcatcccc ttgaatttta aaaacttttt caaaagcaac acctctaaaa
      181 ataaatacaa tatattatag caaatagaaa ttattatttt gttaaattat gttgtcatgt
      241 tcgtgtcatt ttgagtcgta atacaacttt acaattatta aattaacact tctacaatcc
      301 aaaaatattt ttaaagtttt gaaaaaaaat taaagtattt ccactactaa tttggtaaaa
      361 tatgattatg tgatatattg aaagcgtttt acacttaata actccctctt aaatgcatcc
      421 aggttttatg tagtaaatca tgaaaataac atataaatat agaggagatt tacctttgaa
      481 tacagagaac aacaagaatc aaaaccaatc tgttaaaaat tctgaaagac gcggcatgtt
      541 aaaaggatgc ggcggttgcc ttatttcttt tattttatta ataatcttat tatcagcctg
      601 ttcaatgatg tttagtaata atgacaattc cactaataat caatcatcaa aaacgcaatt
      661 aactcaaaaa gatgaaaata aaaatgaaga taagcctgag gaaaaatcag aaacagcaac
      721 agatgaggat ttacaatcaa ccgaagaagt acctgcaaat gaaaatactg aaaataatca
      781 acatgaaatt gatgaaataa caacaaaaga tcaatcagac gatgatatta acacaccaaa
      841 cgttgcagaa gataaatcac aagacgactt gaaagatgat ttaaaagaaa agcaacaatc
      901 aagtaaccat catcaatcca cgcaacctaa gacctcacca tcaactgaaa caaacacgca
      961 acaatcattt gctaattgta agcaacttag acaagtatat ccgaatggtg tcactgccga
     1021 tcatccagca tatcgaccac atttagatag agataaagat aaacgtgcat gtgaacctga
     1081 taaatattaa acaacaagcg aattgaattc aaattgtatt tagctttatg cactaatcac
     1141 atagtaaata atgagggaga ttttttaggc atgagcaatc aattcaaaag cgaagaagag
     1201 cgaagacaat gggaacaatt ccaagctttc caaaatcaac aaaaccaaca gaaccagcaa
     1261 tacggacaaa agaaatctaa aaaaggatgg ttctggggct gtggtggttg tctagtatta
     1321 tttattttaa ttatcatcgg tatttcagct tgtacagctg gtattacagg taaccttggc
     1381 ggaaatagtt ctaaagaaac gaacaaaacc cataaaatcg gtgaaactgt taaaaatggc
     1441 gaccttgaag tcactgtaaa ttcagtggaa actatgaaat ctgtaggacc atctcttgca
     1501 ccaacaaacg ctaaaggtat atttgtcgtt gctgatgtga cgattaaaaa caaaggtaaa
     1561 gaagcgttaa caattgatag ttcaatgttt aagctaaaat ccggtgataa aacatttgaa
     1621 gcagataata caggttcaat gtctgctaat caaagtgaca atggtagtat agaaaattca
     1681 tttttcttac agcgtataaa tccagatagc actgctcaag gtaaaattgt tttcgatgtg
     1741 tcagaaaaca tagccaacgc aaaagataaa aaattagaag ttatttctag tttatttagc
     1801 gtcaagaaga ttacatttga tttatccgat gctaaaaaaa catcaaaagc taaaaaagac
     1861 aagcaagata cagaagtagc tgttgcgagt tcaaatagcg ataatgtaag ttatgaagct
     1921 tcggctacta cacctgctac aacttctagt gcggatactg attctgaaga tagcgaaaag
     1981 tctagtaaag atgaggataa gcagaatgcg tctaaaagtg ataaatctag tgtagaaaaa
     2041 agtgaatcta atgaggaaac tgctcctgta gagcccatgc cccatagcaa acctaccact
     2101 agtgaagcac cacctagcca aaatattcac aatgaagata gcatgtacga cgcttcaaca
     2161 gaataaaatt tcagggtagc tcggctaccc ttcttttacg gaaaaattaa ttatacataa
     2221 tcaaatcaag gagataaaaa aatgaaattc aaagctatcg ttgcaatcac attatcattg
     2281 tcactattaa ctgcctgtgg tgctaatcaa cataaagaaa atagtagtaa atcaaatgac
     2341 actaataaaa agacgcaaca aactgacaac actacacagt caaatacaga aaagtaaatg
     2401 acaccacaag aagccgaaga tatagttcga aacgattaca aagcaagagg cgttaatgaa
     2461 tatcaaacat taaattataa aacaaatctt gaacgaagca atgaacatga atattatgtt
     2521 gaacatctag tccgcgatgc agttggcaca ccattaaaac gttgtgctat tgttaatcga
     2581 cacaatggca caattattaa tatttttgat gatatgtcag aaaaagacaa agaagaattt
     2641 gaagcattta aaaagagaag ccctaaatac aatccaggta tgaacaatca tgatgaaaca
     2701 gatggtgagt cagaagacat tcaacatcat gacattgata ataacaaagc aattcaaaat
     2761 gacataccag atcaaaaagt cgacgataaa aatgataaaa atgctgttaa taaagaagaa
     2821 aaacatgata atggggcaaa taattctgaa gaaactaaag ttaaataatg gcatactttg
     2881 attaatcgta atttttagga gaaacgctat gataacattt gagaatatac aacaacttga
     2941 gaaatacacc ttaatgacta tgcatggtct ttttaatcaa cttaaactcg gcattatttc
     3001 aatcgaaaac gcagagcata cgctttttac accttatatg ttggaaacgc tctcttccct
     3061 aggcgtgaaa gacagcattg tcgatttaat tcataaaggg actgaattag aagactttgc
     3121 ggcatttaat ttatcaattg aagacacagt tacagtctgt ttacaaagaa ctgaagaact
     3181 attaaaacaa tacaaaaatg tggaattcaa tgacaaaata ttaatcaatt ggcgtattat
     3241 acaagagaaa tagacatata aaagtcgaat gtaactacgt gagtattgat tttattcttt
     3301 gtaaattaca agcatttcat attataaagt ttgaaaagag gtatattgaa atggagaaaa
     3361 atgaatatat agctaaatat aatgaatata gtcaattatt agacgctaca tactcgcaag
     3421 ctgtagcata ccttttaagt aaatatggcg ctgtaaccga tgattattat aaagaaaaat
     3481 catacacgcg atttttaaat ggagaaatca aaagtatttc aaaaggaaaa tacactagag
     3541 ctagcgaagg attatattgc catcatataa gcgaggacaa attccaaaat ctatctgatc
     3601 taagattcat ttccaaattt aagtactcat acgacgttca aaagaaagaa aacttagtgt
     3661 actgtgatct aatcgagcat ttaattttac atgcaattat tacaaaagaa tcccatggcc
     3721 aatttggtgt agctggatta tgtcaaatga tcaaaccaac agtcattgat tggtacattg
     3781 gcgaatataa tccaaaacca gcatggatgc aagccaccaa agcacgtgcc tatttgcctg
     3841 gaatattagt agagaaatta ctcattaaaa ttgacgatat gttaaaagga atagaaatat
     3901 aagatttcct tgagtctaga taaatgatta atgtagattt attttttgct gttgagattt
     3961 tgttatagat gtttaaacct gtaattaaat atattttata aaatagacca cgcataccta
     4021 tctataaacg gacaatgttt ataaatgagt ttgcatggtc ttgaattgta ttaaatttct
     4081 tttggtttta ataaatcgac tagattttca caatatttat caaatatgta ttcctaaatt
     4141 atacagcctt aatccagcag ctactttcga aacttccaac ttagttgata taaggttcaa
     4201 tagtttgttt cgttcttttt cagataaacc agaacttaaa ttgatattat tgacttcata
     4261 aaaattatag actaatgcct ctatttgctt tttaggcata agtaagtcga ctgaaaactg
     4321 atttacgtcg ctttcataaa tcatttcatg taaattcttt agactattat cgttactatc
     4381 tctcattaag tctgtatttt taaataaata acggcccaat tcacgagcta ttgaaaatct
     4441 tgtattatta atcgagtgat tattattaat atagattgtt cttccactta aataacccga
     4501 agtattaccc tccattttaa tatatctaac atttaaatta agttgaaata atagcttgtc
     4561 tatgtcaata gcaaagtgtt cagaagtaat aaaaagttga tccattttgt cctttataaa
     4621 tgcctgaaat aatcgaacta tttttgattc taaaatatct tcataatgaa ctttctcaat
     4681 aactttcaat tgattcccca ccctaactaa aaaaatcgaa cctacatcat ttaataaatt
     4741 tataactata ggtgtaaaca ttattaaaat aaaacccctc tactactata tgtaacgaag
     4801 ggacatgatt tcaaaataaa ataccttttt tataaattta ttataatatt tttcattata
     4861 ctatacaacc gaatctaatc taacgatcct gaaaagaaaa ataacggcgg ttccgcttca
     4921 tattcatttt tgtccaagtg ttctttttca attatttgat tgacggtttt ccaatttttt
     4981 acttcatgct cttttaaaaa atggctatga aaaattctat ccccatagat ttcttcatag
     5041 ccaatgctaa tttgtttcat gtcatttctt taaaattcct aaaaatatat aaactaaaag
     5101 cataagtaac acagcaaaaa ttgccgaata tataattatt gaaaaagcat tgctttgcat
     5161 tggaactaaa taatttaaag tacaaaataa taataatgct aaaattccaa ctttaagtga
     5221 atattttaat ctatccataa aatcaccctt actgatatcc ccatttttct cctgtcagaa
     5281 cgtattcccc ggctgcattc tttttagttt ttaattttat atatattccc ttactagtat
     5341 caatattcga tgcagctata gatccaagga aaccaccaac acctgcgcca actaaacctc
     5401 ctgttagtgc agaaataacg ctaatagccc ccgcacctaa agcagctatt gtttttgtat
     5461 atgcagaaga aagatataat gttgcagtat ctttacctgt ttctacatat tgagttttac
     5521 ccgctctcaa ttggtcttca gctttatatt tttttatttc ttcttttgta aatatatctt
     5581 ccagtttata accttttttc tcaagttcat caaataaatt ttggttactc aaatatatta
     5641 cctttgcttg agaatggtct aacttatctt cagcatgagc tacatctgaa ttataagata
     5701 atgaaattgg actaacaaat aatacaccag cagctaataa taaagatttt taattcgttt
     5761 ttcattagtt tcttttagat gatttttgta tttagatttc gtataaacag aaactagatt
     5821 ttttcatgat cgacctatct tttgtccaga tacagtgaga cccttgtcat ttaaatgatt
     5881 tttaattcgt cttgtaccaa agacttttct attagaatta aaaatattta tgacggcatg
     5941 ttctatgttt gaatcatctt tagtgatttt attatctttt ctttttatag aatcataata
     6001 ggtacttctt agtattatca ggactttaca cattgctgat actgaatatt gatgtgcatt
     6061 cttttgaatg acttctattt ttgccccata atcagcgcta cttgctttaa aatatcgttc
     6121 tccattttta aatgttgaac ttctttgcgt aatttaatca gctctttttc ttcatccgat
     6181 aagttatctt ggtgattgaa tgtacccgtg ttttgatgtt gctttatcca ttttcctaca
     6241 ttttataacc gccatttaca aacgtcgaag gtgtgaaatc atactcgcgt ataatttcat
     6301 tccta
//
LOCUS       DANRDI010000148         6259 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.149, whole genome shotgun sequence.
ACCESSION   DANRDI010000148 DANRDI010000000
VERSION     DANRDI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.149"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            71..712
                     /locus_tag="PZ377_002287"
     CDS             71..712
                     /locus_tag="PZ377_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359147.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(800..1090)
                     /locus_tag="PZ377_002288"
     CDS             complement(800..1090)
                     /locus_tag="PZ377_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359148.1"
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
     gene            1369..1656
                     /locus_tag="PZ377_002289"
     CDS             1369..1656
                     /locus_tag="PZ377_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359149.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEV
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            1662..3143
                     /locus_tag="PZ377_002290"
     CDS             1662..3143
                     /locus_tag="PZ377_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDM6359150.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(3232..3588)
                     /locus_tag="PZ377_002291"
     CDS             complement(3232..3588)
                     /locus_tag="PZ377_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDM6359151.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            4065..5024
                     /locus_tag="PZ377_002292"
     CDS             4065..5024
                     /locus_tag="PZ377_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDM6359152.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(5070..5201)
                     /locus_tag="PZ377_002293"
     CDS             complement(5070..5201)
                     /locus_tag="PZ377_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359153.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(5265..5480)
                     /locus_tag="PZ377_002294"
     CDS             complement(5265..5480)
                     /locus_tag="PZ377_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDM6359154.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            5645..6196
                     /locus_tag="PZ377_002295"
     CDS             5645..6196
                     /locus_tag="PZ377_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6359155.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
BASE COUNT         2524 a          727 c         1058 g         1950 t
ORIGIN      
        1 ataaagggca atatgtcata tcgatcacat atgttgaaaa agaggataca ccaaaagagg
       61 taagacaaga atgatagaat taaaagattt aaccatacaa aaaggtaata cacatatttt
      121 gaagaaactt aatttgaaat ttcaatgtgg aaaatcatat gcacttattg gtaaaagtgg
      181 atgcggtaaa tctacattat taaatactat tgctggactt gaaaaaacag gaaaacaata
      241 tgtctatttt aatggtcaat tagaacaatt taaatctaat ttttacagag ataaattagg
      301 atatttattt caaaattatg gattaatcga taatttgaca gtaaatgaaa atttagatat
      361 tggattagca tataaaaaaa taagtaagaa agaaaaagaa caaataaaga tacgttatat
      421 agaacagttt ggtctgtcaa acagtttaaa aagaaaagtt catacgctaa gtggaggtga
      481 acaacaacgt gtcgctttaa ttagaatgat gttaaaagat ccgattgtta tgttagctga
      541 tgaaccaacg ggtgcgttag atcctaaaac aggacagatg attattcaat cattatttgg
      601 tttggtcgat gaaaataaag tgctgatttt agcaacacat gatatggcta ttgcaaatca
      661 atgtgacgaa ataatagatt tagaacagta tagaaaagta gcatctatgt gatatccaaa
      721 agataaaatt aaaagccaat caatgggaaa tgttgacttc aacactccgt ttgattggct
      781 tttatactgt gttactaagt tatgaaagta acaacggcaa actgaaaaaa agcaatataa
      841 taatatttaa tacgattagt aataaatgta attgattttt cgttttaata tagcaaaaaa
      901 caatgactaa agataacaac aatatcatga ttgcacctgg gaagaaccat aatgctgaag
      961 ctgtgtacca aatcattggt aacgccataa taataaaaga tataagacaa gaaaggatac
     1021 caataatatt ataagttcta gtcaaaatta cacctccaat tttcacttta aaatgacatg
     1081 cgtaaaacaa tcattttaaa tacattatac aataaaatta aattgtgcgg tagaagaatt
     1141 tgaaattaaa ttgaattgtt tttggtagtt ggtatattta taacactctg caaaagaatg
     1201 tagcaaaatt agataaatta gattatttaa aattatgcgt gatattatta tttttatcat
     1261 gcaaataatt ataataacaa tatttaatta ttgtatttac agttgattaa tagcgttgtg
     1321 aagtataaaa ttattttgta aaacaatttt gttattaaga ggtgcgctat ggaaaagggg
     1381 attttcaatt acgataatgc gaatgtatta aaattagata ctaatcagtt aaatgaaaac
     1441 ataaaagtca ttgacgatat atttaaaaat tatgagcaga tagagccaac tatagaagtc
     1501 gaaaatggaa atacaaaatt aaaattaaat ggatatttta ttgcgtcaat tataagccca
     1561 ttaaatttaa ataaacttaa taatttatat gttgaagaag aattttatca tacatataac
     1621 gaattaattg ttaaatatac tgaggtaaag gaataattat aatgaaaaaa atatttatga
     1681 tggtacatga gttagatgtg aataaaggtg gtatgacctc ttcgatgttc aatagaagta
     1741 aagagtttta tgatgcggac atacctgctg atattgttac tttcgattac aaaggaaact
     1801 atgatgaaat tattaaagct ttgaaaaaac aaggtaaaat ggatcgaaga acgaaaatgt
     1861 ataatgtatt tgagtatttt aaacaaattt caaataataa acattttaag tctaataaat
     1921 tgttatataa acatatttca gaaagactaa aaaatacgat tgaaattgaa gagagtaaag
     1981 gtatttcaag atattttgat ataacgactg gtacatatat tgcctacatt agaaaaagta
     2041 aatctgaaaa agtgattgat ttctttaaag ataataaacg aattgaacgg tttagtttta
     2101 tagataataa agtgcatatg aaggaaacat ttaatgtaga taataaagtt tgttatcaag
     2161 tattttatga tgaaaaggga tacccatata tttcaaggaa tattaatgct aataatggtg
     2221 ctgtaggtaa aacttatgtg ttagttaata aaaaagaatt taaaaacaat ttagcactgt
     2281 gtgtttacta tttagaaaaa ctaataaaag attctaaaga tagtattatg atttgtgatg
     2341 gaccagggag ttttccaaaa atgtttaata caaatcataa aaatgctcag aaatatggcg
     2401 ttattcatgt taatcatcat gaaaatttcg atgatacggg tgcatttaaa aaaagtgaga
     2461 aatatattat tgagaatgcg aataaaatta acggtgtaat tgtattaaca gaggcacaaa
     2521 gattagatat tcttaatcaa tttgatgtag aaaatatttt cactattagc aattttgtta
     2581 agatacataa tgctccaaaa cattttcaaa ctgaaaaaat cgtaggtcat atttctagaa
     2641 tggtaccaac gaagcgaatt gatttgctta ttgaagtggc tgagttagtc gtaaaaaaag
     2701 ataatgctgt taaatttcat atatatggag aaggatctgt caaagataaa atagctaaaa
     2761 tgattgaaga taaaaattta gaaagaaatg tttttcttaa aggatataca acaactccac
     2821 aaaaatgctt ggaagatttt aaattagtcg tttctacatc tcaatatgaa ggtcaagggt
     2881 taagtatgat agaagcaatg atttctaaaa ggcctgttgt tgcctttgac atcaaatacg
     2941 gaccaagtga ttttatagaa gataataaaa atggttattt aatagaaaac cataatatta
     3001 acgacatggc tgataaaata cttcagcttg ttaataatga tgtattagca gcggagtttg
     3061 gttcgaaagc gagagaaaac attatagaaa aatattcaac ggaatcaata ttagaaaaat
     3121 ggttaaatct tttcaatagc taaatgaaaa aatcctaggt attcatgagt acctaggatt
     3181 tttacgcatg ataaatacaa aaaaagagag gatagtcctc tcaaacaata gttaataaaa
     3241 ttgtagccat ttcttccagc tgtaactact aatacaagct aagttcatta cgatatgtat
     3301 gatttcaaat tcataaccac ttacatatcc tcttgtaagt ttaagtgtga agattgagcc
     3361 taacataaca caaatataaa aaacattaac ataaggcgtt aaaaagccga taataagtgc
     3421 taaacctcca acaacttcac caattgacat caatactgcg atggtaggtg gtaaacctaa
     3481 agagcctaag aagtgaattg ttccatctaa atcaacaaat ttcaaagtac catgcactaa
     3541 gaatgatgtt gctaacatcc atctaattaa aagcatacct aatttcatca tgtcacctcc
     3601 aacattcata tttttaaaac atagtccaaa ttatatttgt aaacatgtgg aaagtaaaga
     3661 ttttttataa gattaattat taaatattct attgtattga acaattgttg tagaaagatg
     3721 tttaagtcat cagttacttt ttgtatttat aaataaagat tagtacagca tagtgcaaag
     3781 attataggga aatgaattaa ctattgcatc gctatttatg aatcgtaaac actattatga
     3841 aattgcgaga gtagtatgga caattagaat gatggtttga gataaatgag tgttattaac
     3901 cagtgcgtca ttttcaaaat tagatgccat ttgctgttta taataagaag atttgataat
     3961 taactatgat aagaaaaaat aataatcccc taaatacatt ataaacaaat taaatgataa
     4021 aataataata tatatttcta ctaaagttgt ttggaggtaa atgtgtgaat aggaatatcg
     4081 ttaaactagt tgtgttcatg ctaattttag ttgtagcagt agcgggttgt ggtcaaaaag
     4141 atactgaaga gaaaactgaa atgacgacaa taaaagatga attaggaact gaaaaaatta
     4201 agaaaaatcc taaacgtgtt gttgtattag aatatagttt tgctgattat ttagcagcat
     4261 tagatatgaa acctgttggt attgcagatg atggcagcac taaaaatata acaaagtcag
     4321 taagagataa gattggggca tatgaatcgg ttggatctag accgcaaccg aatatggaag
     4381 tgataagtaa attaaaaccg gatttgatca ttgcagatgt tagcagacat aagaaaatca
     4441 aatcagaatt gagcaaaatt gctccgacaa tcatgttagt tagcggtacg ggagattata
     4501 atgcaaatat tgaagcattt aaaacagtcg ctaaagcagt aggcaaagag aaagaaggcg
     4561 agaagcgtct ggaaaagcat gataaaatat tagcggagat tagaaagaaa attgaacaga
     4621 gtacgttaaa atctgcattt gcattcggta tctcaagagc aggtatgttt attaataatg
     4681 aagatacatt tatgggacaa ttcttaatta aaatgggtat tcaacctgaa gtcacaaaag
     4741 acaaaactac gcatgttggt gaacgcaagg gtggtcctta tatatattta aataatgaag
     4801 aacttgccaa tatcaatcca aaagttatga ttttagccac tgacggaaaa acggacaaaa
     4861 atagaacgaa attcattgat cctgcagttt ggaaatcatt aaaagctgtg aaagataaca
     4921 aagtttatga cgttgaccga aataagtggt tgaaatcaag ggggattatc gcaagtgaaa
     4981 gtatggcaga agatttagaa aaaattgcag aaaaagcaaa ataaaaatac agcgctactc
     5041 gtaaatcata taagagtggc gctgtatttt taattatgtt tattatctgt cggatgtgat
     5101 gatttacctg aaagtttatt tcgaataaat ttaattacat aaccgacaag gattgtttta
     5161 acagttcttt taatgaattg gcgcatcgtt acatacctca tttctctata tcttacgaac
     5221 tatataccca ttcatatatg ctttttaaac gtcattgtca caatttaatt tttagggaat
     5281 ataatataac catctttatc tgctttttta gtaaaaatga caaaaattgc atgtattatt
     5341 gagatgatgg tagggatacc tgtccagaaa aataataagt gaaaaagacc ttgtccaaat
     5401 ttatcagcat aaaatttatg aatacctaaa cctccaagaa ataatgcaac aataacataa
     5461 atggctttat tgactttcat ttgtaaatcc tccttaacta taattctact taaattcgtt
     5521 gtgaaaacca atatttctaa ctttagaatt ttcaaacttt ctaaaattat aagtatatct
     5581 ttttaaaata agctagaatt tctatataat aaatgttaat aacgtaaaag ggaatgatga
     5641 catagtgata cgtcaagcac gtccagagga ccgatttgat attgcgaagt tagtttatat
     5701 ggtttgggat gatatggaat tagaattggt aaagcatcta cctaaagaca tggtattaga
     5761 tgcaattgaa aaaagctgtg ttgatgcaac atatcgaact ttttatcagc atattttagt
     5821 ttatgaagta gaaaataaag tagcaggttg tattattagc tatagtggtg aaaatgaatt
     5881 gaaatacgaa aaagcatggg aactacttga cttgccagaa aaaataaaac aatatggcac
     5941 gccattacct gtaaaagaag ctaaagacga tgagtattat atagaaacaa ttgcgacatt
     6001 tgcagcatat agaggtagag gcatcgcgac aaagttatta acgtcattac ttgaatcaaa
     6061 tacacatgtt aaatggagtt tgaattgcga tattaataat gaagcagcat taaagttata
     6121 taaaaaagta ggctttatat ctgatggaca gattgaatta tacaagcaca tgtatcatca
     6181 tttaattgtt aaataaaaaa actcgacagt tcgatgtaag tcgattgccg agtagtatca
     6241 ttttctatta aatgcctgc
//
LOCUS       DANRDI010000149         6221 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.150, whole genome shotgun sequence.
ACCESSION   DANRDI010000149 DANRDI010000000
VERSION     DANRDI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.150"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(10..762)
                     /locus_tag="PZ377_002296"
     CDS             complement(10..762)
                     /locus_tag="PZ377_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDM6359156.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            813..1142
                     /locus_tag="PZ377_002297"
     CDS             813..1142
                     /locus_tag="PZ377_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359157.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            complement(1131..1346)
                     /locus_tag="PZ377_002298"
     CDS             complement(1131..1346)
                     /locus_tag="PZ377_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDM6359158.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1362..1625)
                     /locus_tag="PZ377_002299"
     CDS             complement(1362..1625)
                     /locus_tag="PZ377_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6359159.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            complement(1622..1795)
                     /locus_tag="PZ377_002300"
     CDS             complement(1622..1795)
                     /locus_tag="PZ377_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDM6359160.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            1758..2471
                     /locus_tag="PZ377_002301"
     CDS             1758..2471
                     /locus_tag="PZ377_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6359161.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVKLIGKVIL"
     gene            2487..3419
                     /locus_tag="PZ377_002302"
     CDS             2487..3419
                     /locus_tag="PZ377_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDM6359162.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVE
                     SVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDV
                     NGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            3425..3766
                     /locus_tag="PZ377_002303"
     CDS             3425..3766
                     /locus_tag="PZ377_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359163.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            3970..4152
                     /locus_tag="PZ377_002304"
     CDS             3970..4152
                     /locus_tag="PZ377_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359164.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            4230..4943
                     /locus_tag="PZ377_002305"
     CDS             4230..4943
                     /locus_tag="PZ377_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDM6359165.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIEN
                     KLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSD
                     IMLLINRINNNILQIPDIR"
     gene            5134..6171
                     /locus_tag="PZ377_002306"
     CDS             5134..6171
                     /locus_tag="PZ377_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDM6359166.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
BASE COUNT         2285 a          912 c          951 g         2073 t
ORIGIN      
        1 ttcctccttt taagatgttt gtttttctcc taaaaactta ttaacaaagt attgttgtcc
       61 tttgcctgtt acttttggcg tcttactaat tgatgtgtga ccgtccgaat gtgtgattga
      121 tgtttcttta atttcgaata actcacgttc cattgaatac tgtgtaggca tgttataatc
      181 cacacccttg cgtttaataa ggaatccgtt ttgacgtaac cactcaaaca atctgcgttg
      241 cccgatgttt acaccgtttt gtttaatgat ctttgctaac tctccaacta aaattgatgt
      301 cttagtagta gctactgcat ctgcaaatac aatttttggt ttatcacgtt caatctttgt
      361 ttctaattga ttgattgtgt tgttagcaat ttttaaagca cgttgcataa tcatttctgg
      421 gctgttccat gctttttcaa cttggatgaa gtattgtctt gcacgtttac caggttcact
      481 acgttgaatc attgcgattt cttttgcagt gtctagtgtg agtgcgtggt caatatagtg
      541 tgtcatattg ccttgagctg ttgctctttt ttgagcgata gctgtgtaat ctgtattttc
      601 ttcaaatccg tatttaagca ttcttggaaa ccaatcttta tatgctgtct taacttctaa
      661 tgcttgatga agttctcgac cgctgattgc gatttctcca ttttcttttt cttgaatatt
      721 gaacatttct ccgatgttcg aatttgtttg taatgcttgc atattgttta tgctcctttc
      781 tgctatactc ctattaagga ggtgaatgac ttatgactaa tgaagctaaa tttgtccttt
      841 tacaacttta ttcaatttac cttgatagaa ttgacgaagg tatgtctaaa cgttctgcat
      901 cttatttcgg tagtgatgaa tcctcattta acgctttctt tttagatttt aattttgaag
      961 actatatcga tgcagttctt gaattaaagc atagagattt tgtaattgct tctgctgaag
     1021 atggcggttt tcttgagatg gctctttctc gagaaggtat cgcctactca gaatcagaat
     1081 ccaaaaaaga ttacaaaaca cttatggatt taattagaga tttgaaaaaa ttaataatct
     1141 aaaatccaat catctgctat taaatcgtct gcgctaggct gccaccttcc ggcggcggtt
     1201 tgtctttttt tcttatagtg tctagatacg actaggcatt gataacgctg caaattagtt
     1261 ggtaatatcc catacacatc ttgattctct ctccttatac ttattccttt ctccatcgct
     1321 agcttcgttg cttcttgaat gttcatttgt tattcctcct attaagatgt ttgtttttct
     1381 ttaaatgcta aaataattga tttcttttta tcattcgtaa atacgaaatt ttcgtattca
     1441 ttacctaaaa aaatatcatc atatttaaca ttaaaagcac tcatatactt agaaagtaaa
     1501 ctatctttaa tgtttgtaga gtctttttcc atattttgaa ttgtacgtga tgagacctta
     1561 aataaatctc ctaactcttt ttgagtcaat ccgtaatcag tcctcaactc ttttaatgtt
     1621 ttcatgttgt caccgccttt cgtaaaccta atataatacg aaattttcgt attgtcaaca
     1681 ttaaatacgt ttttttcgta aaaaacttta ctatgatatg aaaatttcgt ataataagaa
     1741 aaaaggaggt aagtaatatg aacaaagaaa gaaatattat tatagccaaa aacattagaa
     1801 aatttctcaa cgattcaaat atgtctcaaa agaaacttgc tgaactcatt aacataaaac
     1861 catctacttt aagcgattat ttaaatttac gttccaaccc ctctcacggc gttatacaaa
     1921 ggatagctga tgttttcgag gttggtaaaa gcgacataga tactacatac aaagacgata
     1981 acgacatcac ttccatatac aacaaactca cacctccccg ccaagaaaac gtacttaact
     2041 atgcaaatga acaattggaa gaacagaatt ctaaaggaga taacgttgta gatattaatt
     2101 catataaaca ggagaaaact ccagttaacg tcaatggttg cgtctctgct ggtgtaggag
     2161 aacgtttaca cgatgaaacg ctatttactg aaatggttaa aggacctatc cccacacacg
     2221 atttagcgtt aaaagtaaat ggtgattcta tggaacctat gtttaaagat ggcgaaatca
     2281 tatttgtgga gaaaactcac aatataaaga atggacaaat tggtatattc atcattgaag
     2341 aagaagcgta cgttaagaaa gtctttgttg aagatgatag attgactcta gtttcactaa
     2401 ataaagatta cgacgatcta cacttttata gaaatgaaag tgtgaagtta attggaaaag
     2461 ttattttata aaaggagcac ttgcaaatga aaaaatatga tattgcagtc ttagactttg
     2521 aaactatgaa tgaacatatg aacagccctt gcgaagttgc tgtatcttta attaaggatt
     2581 tatcaatagt aaaagtttat tcatcttata ttaatcctcc taataataga tataacttga
     2641 aaaacgctaa aatacataaa atacctgaag atgtcatatt aaaagcacct aaatatccag
     2701 atatttacca agaaattctc tatcttttaa aagaatcaca tttaattatt gctcataatg
     2761 cactttttga tatttcagta ttaaaaaata ctaataatta ttatgactta cctgttccaa
     2821 acttcatgta tgtcgatagt ataaatatct ttagaagctt ccacgcaatc tctagtttta
     2881 aattagaaaa tttgtgtagc ttatatgata tcgataaaga aaaattacat tctgctaaat
     2941 ttgacgtgct agctttatcg aagatgttga tatcactcgc taaaaacaat cagcattata
     3001 gtgtattaaa attaatacat tatatgccta agcaatacat tagatttagc aaatattcta
     3061 actctccaac taaacttttc gattcaggat ttcaaaaaat tcatatgaaa atatctgaga
     3121 ttaataaaat agaagtggaa agtgtaatcc ctattttaaa agataaaaat gttgttttta
     3181 caggtaattt tgacactgaa aaacaagatt taatgatatt aactagaaag aaaggagctt
     3241 atatcagaag tgacgtaact gcaaaaacag atattttagt cgaaggtgtt caagatgata
     3301 aatataaaga tgtgaacgga ctagtttcaa aacaacgaaa agctcgagaa tatgttggaa
     3361 atggtgcaaa aattcaattt ttaaatgaag aagacttaat aaatttaata aaggaataat
     3421 aacgatgatc aaaaaaattt ttacaaaaaa gcatgtattc ttagttatag aagatgaaaa
     3481 ccataatcac agtgatgctg tttttggaaa aagtatatta ctttcaattt acgtcggtgt
     3541 gaataaaaag actaattcta aatcagggaa atttatatac cttgacagat ctaaaagaat
     3601 cgttagacaa tctgatatca ccaaaataga atcagctaac gaaaatgatg tagattttta
     3661 taatttactg aagaaagaaa aggaaattgt ttattccaaa aatatagtag ataaatacaa
     3721 tttagcgaac tatataattt actacgaagt tagtactaaa gaataaacca atccattatt
     3781 tcataatact aaccttaaat ttacagaggt tttaattatg aaacatgaaa aaagcaatct
     3841 taactttaag tcttatattt attacctact acctcacttt taaatatatg tggattaaag
     3901 aattgaagta ttaattatgc ttatttaaaa aagacgtcta tttcagcagt gtttgaaagg
     3961 aagtttataa tgaaaataac taattgcaaa ataaaaagag aaactgtaat atacgaagtt
     4021 ttaactagtg gtaatcaacc attcacttat gagttaccta aagatttatc gtcacataat
     4081 gcgcgtaaat acttggaatt tatttcacaa aaaatagatg gcgataagtt aaccaaagaa
     4141 gattcattat gattttacta atcaaaaaac gtctacaagt gtagacgttg aatggtggtg
     4201 agagtgtgag cgagaataaa ggagaaatga tgacgcataa tatagaaaaa cgcattaata
     4261 aattaaaaac ttctggaaat ccaaaattta aaaaattaga ttcagatatt cactatttac
     4321 tcaagagatt tgaaggtgaa aaaaaccata aaggttttta tccaaagttt aaacaaggag
     4381 aaatagtttt tgtagatttc ggtataaacg ttaataaaga attctctaat tcacactttg
     4441 caatagtgat gaataaaaat gattctaata cggaagatat agtaaatgtt attcccttat
     4501 cttctaaaga aaacaaaaag tatttaaaga tgaattttga tttgaaatgg gagtattatt
     4561 taagattgtt tttaaattta attagcgcgc aaaataattc agctatatta aaagaagttt
     4621 tcgataaaaa ataccaaaaa aacaacacag aattcatcac taaagattat tttagtgaat
     4681 ttatatctga tagtttagaa attgaaaata aattaaataa aattgacaga aacattaata
     4741 acatagtatc agcaattgat aaggtaaaaa aattaaaagg taatagttac gcttgcataa
     4801 attctttcca gccgattagt aagtttcgca taagaaaagt tttaccccaa aaaattaaaa
     4861 atccagtaat agattcttcg gatattatgt tactgataaa tagaattaat aataatatat
     4921 tgcagattcc tgatataaga tgatataatt ttaatatatt aaaggtttat cctttaaaac
     4981 acgtatatat tcgttaccat ttttggtaat taaccatgta atcttataac tataagtggc
     5041 gtctgtattt tatacaggcg tcttttttta tacaattttc atgggtagcc cgcctaccct
     5101 tattattttt tgccaatttt gaggagggaa cgcatgaaaa cacgttgtta cgatggtaaa
     5161 aaatggcaat atgaatttaa gtatgaagga aaaagatacc gtaagaaagg ttttagaaca
     5221 aagcgtgaag ctaattctgc tggactagac aagttaaatg agttaagaag tggttttaat
     5281 atagataact atataactct tgaagaatac ttcgaaaatt ggattaaaac gtataaacaa
     5341 cctgttgtta aagaaaatac ctaccgtcat tatagaaatg cattacaaca tatacaaaaa
     5401 cataaaatag gtaaaatgga gttatcaaag ataaatagac aagtttatca gaaattcata
     5461 aacgattatt caaaagaaca cgcaaaagaa actataagaa aaacaaacgg tgctattcgg
     5521 tcagctttag atgacgcatt atatgatggg cttattttta aaaatcccgc ttataaagtt
     5581 aattataaag ccggaaaacc tacgaagtca gaacaagaaa aattcatctc ggtaactgaa
     5641 tatgaaatac taaaagatca cgtcagaaag aagagaactc gttcatcatt agcgctattc
     5701 ataatgattt gtacgggttg tcgtgtcagt ggtgcaagaa atataaagat tgagcatatc
     5761 aaccaagtga aaaacactat atttattgac gagcgaaaaa ccgatacttc ccctagatat
     5821 atcagtatcg ctaaatctga tatgaaacac attatggacg tcataagtac atttgcaatt
     5881 agctatgatg gttacatttt caaagaagcc ggatctataa ttaaccttca tgctatcaat
     5941 aatgctttga aatcagcctg tagagtcaat aatatatcaa ttattacatc gcacgcatta
     6001 agacacactc attgttctta tttactagca aaaggtgtat ctatacatta catttctaaa
     6061 agattaggtc ataaaaatat agcaataact acatccgtgt attctcattt gttagaagaa
     6121 aaatttaatg aagaggacaa aaaaacaact aaaattttag aaagtatgta atttagggac
     6181 ccattaggga ctccaaaccc aataaatact gttgttacaa g
//
LOCUS       DANRDI010000150         6208 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.151, whole genome shotgun sequence.
ACCESSION   DANRDI010000150 DANRDI010000000
VERSION     DANRDI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.151"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1998
                     /locus_tag="PZ377_002307"
     CDS             <1..1998
                     /locus_tag="PZ377_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HDM6359167.1"
                     /translation="SRNNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDITGFGTTIF
                     QEEETDGNNIVMLKQKFLHALRNNDEIVINEKDVESLINDNEINHYHAPMSADVYAEL
                     YLGRFYNQYNELIVISPLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYYQKMICDD
                     NVEMISINNIPKYPRNHNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVGATFNKLY
                     LYSSQLNKRVLFESNNMYNFLKECNLYRLLREISMESVKCIEPMNDVSIDSFSYSPRI
                     RYKNVILKPAYWKINEMVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDNKLLLNLS
                     LANHRYLLMKEYKKHKRVRLVESFLPQSKNDHVYEIVTPIYKKSSYRGPEIEIPKYKN
                     TDIEYDKDWFALHIHIDKPSQDTFIIDNLYPFVKHLKDKGDIDQYFLMRYIKQGDILK
                     LRLFRNDENYAEIYSILKNWLPHVRQTTEVSDYEFVSYEPEFFRYGGKNTINEIEAFF
                     EYDTNLAVNIIENDFKFDRPYIVAISIMYLFEMFSISNEERMEIVNNYVPTSFKSKDI
                     RPFKNELVTICNPANNFEYMAKHYSGIYRILKDGNQILSKLNEGLKKTLTTKRSRIIG
                     SLIHMRCNRIFGIDKDQETFVLSIVKEIVKTQKHWCGDKND"
     gene            1991..3235
                     /locus_tag="PZ377_002308"
     CDS             1991..3235
                     /locus_tag="PZ377_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HDM6359168.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            3251..3769
                     /locus_tag="PZ377_002309"
     CDS             3251..3769
                     /locus_tag="PZ377_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HDM6359169.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            3779..5152
                     /locus_tag="PZ377_002310"
     CDS             3779..5152
                     /locus_tag="PZ377_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDM6359170.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            5175..5867
                     /locus_tag="PZ377_002311"
     CDS             5175..5867
                     /locus_tag="PZ377_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HDM6359171.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            5864..>6208
                     /locus_tag="PZ377_002312"
     CDS             5864..>6208
                     /locus_tag="PZ377_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDM6359172.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCF"
BASE COUNT         2370 a          776 c         1059 g         2003 t
ORIGIN      
        1 tcaagaaata atattggatt cacagatttg aaagttcttc acaaccgttt tattgaaaag
       61 tatggatttg aacaactcgt gaatattaaa gatttgattt cagatataac aggtttcggg
      121 acaacaattt ttcaagaaga agaaaccgat ggaaataata ttgtaatgct taagcaaaaa
      181 tttttacatg ccttgagaaa taacgatgag atagtcataa atgaaaaaga tgtagagtca
      241 ttgattaacg ataatgaaat caatcattat catgcgccaa tgagtgcaga tgtttatgct
      301 gaactttatt taggacgttt ttacaatcag tacaatgagc tcattgttat tagtccttta
      361 acggcgtctt ttaatgctgg cgctacattt gggcgatttc atcatttaat tgatactgaa
      421 actttagcaa aattagaaca tgaaaaagga cattattatc agaagatgat atgtgatgac
      481 aatgtagaaa tgatttctat aaataacata ccgaaatatc cgagaaatca taatgtatta
      541 actaatcatg actcatacga atattcattg aatttaggaa gtagtaatag ttattcaaag
      601 tatgagctta ccttagatga tatttatgtt ggtgctacct ttaacaaatt atatttatat
      661 tctagccaac taaataaaag ggtactattt gaatcaaaca atatgtataa ctttttaaaa
      721 gaatgtaatt tatatcgatt gttacgagaa atttcaatgg aatctgtgaa atgcattgaa
      781 cctatgaatg atgtgagtat tgattcattt agttattcac cacgaatcag atataaaaat
      841 gtcattctta aaccagcata ttggaaaatt aacgagatgg tattaccttt acctaaaaat
      901 gaagaatggg atcaacaatt tttgaaatat caagaacaat tcaatatacc taatatcgtg
      961 aatttagttt atggagataa caagcttcta ctaaatttat cattagctaa tcatagatat
     1021 ttattgatga aagaatataa gaagcataaa cgtgtacgtt tagtagaatc atttttacct
     1081 caatcaaaga atgatcatgt gtatgaaatt gttacaccaa tttataaaaa atcatcttat
     1141 cgaggacccg aaatcgaaat tccaaaatat aaaaatacag atattgaata tgacaaagac
     1201 tggtttgcat tacatattca tatcgataag ccatcgcaag atacatttat catcgacaat
     1261 ttgtatccat ttgtaaaaca tcttaaagat aagggagata tagatcaata ttttttaatg
     1321 cgttatatca aacaaggtga cattttaaag ttgaggttat tcaggaatga cgaaaattac
     1381 gcagaaatat acagcatctt gaagaattgg ttaccacatg ttcgacaaac gacagaagtt
     1441 tcagattatg aatttgtatc atacgagcca gaatttttta gatatggtgg aaagaacacg
     1501 attaatgaaa tagaagcctt ctttgaatat gacacgaatt tagctgtcaa tattattgaa
     1561 aatgatttta agtttgatcg accatatatt gttgctattt caataatgta tttatttgag
     1621 atgttttcaa tttctaatga agagcgtatg gaaattgtta ataactatgt acctacatca
     1681 tttaaaagta aagatattag accttttaaa aatgaacttg taactatatg taatcctgct
     1741 aataattttg aatatatggc taaacactat agtggtattt atcgaatttt aaaagatggc
     1801 aaccaaattt tgagcaaatt aaatgaaggg cttaaaaaaa ctttaactac taaaaggtca
     1861 aggattattg gcagcttaat tcatatgcgc tgcaatcgaa tatttggcat tgataaggat
     1921 caagaaacat ttgttttatc tattgtaaaa gaaattgtta aaactcaaaa gcattggtgt
     1981 ggtgacaaaa atgattgata tgaatctaat attgaagaag aagtttcaag agatttcaga
     2041 agtagatgat ttcatttcaa aagcatctac tgaaaccgat tattttgaac cttcaacttt
     2101 atcacatggt ataccaggta tcattttgtt tttagatgct tatcaaaaag ttttcgatat
     2161 taatactgag caaattgtgc ataagtacat catgaaatta gcaccatatt tacagagtgg
     2221 tcaatataac aattctttgt ttggtggact ttcaggtatt gcattttcaa tggatttagc
     2281 atcgcaaaat ggcaaaaatt atcaaaatat tttaaataat attgatggtc acattgtaaa
     2341 tgaaatagaa aataaatttg atcaaatatt acaagaacca ttaaatccat taaattatga
     2401 tactgtcagt ggattagctg ggatagggag atatttgcta aatagagtag atgagaatga
     2461 atttaatgtt aaagcattaa aaagcatatt agtatacttt aaagatattc aatattctaa
     2521 aaatagctgg gtagtcccac aagaaagtca atttttagag tctgataaaa attattttac
     2581 tgaaggtaat atcaatcttg gccttgcaca tggagtgcta ggaccgatgt ctttatttgc
     2641 attatgcgtg attaaaggaa ttacgattga aaatcatcag cacatattaa aagacatgta
     2701 caaatttatc atggacgaaa aattttgtaa ccacgaaaga tggttgcagc gttacgattt
     2761 aatttctgaa cgtaatcatt tcaattttat tcggaatggt tggtgttatg gcaatacggg
     2821 tgtaatgacg acgttgtttt taatcggcca agcattacaa gatgatgaaa taattaaaat
     2881 gtctaaaaaa gtgatgctac aagtagtaaa tgataaagat gaaaatttaa taagtccaac
     2941 tatttgtcat ggattgtcat cacaaatatt aatgttaaca attatgaatt tgaattttga
     3001 attaaatgaa gtgtctgatt atatcactgt attaataaat aaactgattt ctcattataa
     3061 ggaagattat ctggtgaatt ttatagacat taatgaaaat aagcaagatg tatttaaaag
     3121 taggaaagtt ggccttttag aaggtgaatt aggggtctat ctaacattga tgacactgaa
     3181 aaatacaaaa attctcaatg aaaaaaattg gaccaatgcg tttttaatta gctgaatgga
     3241 ggcgtatatc atgggagaaa atgtattgat ttgtttgtgt ggctcagtta atagtatcaa
     3301 tattagccac tatattattg aattaaaatc aaaatttgat gaagtaaatg taatcgcgtc
     3361 aacaaacggt agaaagttta ttaatggtga aatattaaag caattttgcg ataactatta
     3421 tgacgaattt gaagaccctt ttttaaatca tgtagatata gcaaataaac atgacaagat
     3481 tattatttta cctgcgactt ctaatacgat taataaaatt gcaaatggta tatgtgacaa
     3541 tttattatta actatttgtc atacagcttt tgaaaaactt tctatatttc caaatatgaa
     3601 tttacgaatg tgggaaaatc cagttactca aaataatatt cgattattaa aagattatgg
     3661 tgtatcaata tatccagcaa atatttcaga aagttatgaa ttagcgtcaa aaacatttaa
     3721 aaagaatgtt gtcgcacctg aaccatataa agttctggaa ttcatttgag attagaatat
     3781 gaaaatcata aagcgcgcta ttattagttt aataatattg agtcttttaa tatctattac
     3841 aatgagtaat gcatcagcgt cagaagaact atattacagt gttgaataca aaaatacagc
     3901 aacatttaac aagttagtta aaaagaagtc cttaaacgtt gtctataata taccggaatt
     3961 acatgtggca cagattaaaa tgacgaaaat gcatgctaat gctttagcaa actataaaaa
     4021 tgatattaaa tatatcaatg ccacatgttc aacttgtatt actagcgaga aaacaataga
     4081 cagaacatca aatgagtcat tattttcaag acaatgggat atgaataaaa taaccaataa
     4141 tggtgcatcg tatgatgatt tgccaaaaca tgctaacacc aaaatagcaa tcatagatac
     4201 aggtgtgatg aaaaaccatg acgatttgaa aaataatttc tcgactgatt ctaaaaattt
     4261 agtaccttta aacggtttta gaggtactga accggaggaa acaggtgatg ttcacgatgt
     4321 caatgatagg aaaggacatg gcacgatggt gtcgggtcaa acgagtgcta atggtaagtt
     4381 aataggtgtt gcaccgaata acaaatttac aatgtatcgc gtgtttggta gtaaaaaaac
     4441 agaactgctt tgggtatcaa aagcgattgt tcaagctgca aatgatggaa atcaagtcat
     4501 taatattagt gttggtagtt atattatttt ggacaaaaat gaccatcaaa catttagaaa
     4561 agatgaaaaa gtagaatacg atgcgttaca gaaagcaatc aattacgcca agaagaaaaa
     4621 atctatcgtt gttgctgcag ctggtaatga tggtattgat gtcaatgaca aacagaaact
     4681 aaaattacag cgtgaatatc aaggtaatgg cgaagtgaaa gatgttcctg catctatgga
     4741 caatgtcgtt acagtaggat ctacagatca aaagagtaat ctatctgagt tttccaattt
     4801 tggtatgaat tatacagata ttgctgcgcc cggaggatca tttgcttatt taaatcaatt
     4861 cggtgtggat aaatggatga atgaagggta tatgcataag gagaacattt taactactgc
     4921 caataacgga agatatattt atcaagctgg aacttcatta gccacaccta aagtttcggg
     4981 agcactagct ttaatcattg ataaatatca tcttgaaaaa catccagata aagcgattga
     5041 attgttatat cagcatggga catctaagaa taataaacca tttagtagat atgggcatgg
     5101 tgagcttgat gtgtataaag cattaaatgt agcaaatcaa aaagcaagtt aataaatcaa
     5161 aggagttttt gattatggca aaattagtta ctgaaaacat ttcgaagcgg tttaaaaatc
     5221 aagatgtatt aaagcatatt aatatcactt tagaaaataa cgaagtttat ggattacttg
     5281 gtattaatgg agccggtaaa acgacactta tgaaaattat atgtggcata cttcaacaag
     5341 attcagggga aattaaatta gataatagac caatgacacg aaatgatttg cacaaagttg
     5401 gttcgcttat tgaaacacct gcgacatata atcatttaag tgcacaagat aatttgaaaa
     5461 ttgtgtgttt aaatgaaagc gttgatttca gcgaaattaa tagtgtttta agcttagtca
     5521 atttaaatgt cgataaaaag aaaaaggtta aggacttttc tttaggtatg aaacaaagac
     5581 ttggaattgc aatggcatta attaaaaagc cagaaatttt agtattagac gaaccatcta
     5641 atggtttaga cccatatgga atccaagaac ttagagaact tctaaaatta ttaacagaac
     5701 aaggtactag tattattatt tcaagtcaca ttttatctga aatccaagtt ttagcagatc
     5761 atatcggtat tattcatgag ggtgagctaa aatatcagca aagaaataac aaagatgaaa
     5821 acttagaaga gatattcttc aaaataacga aaggtgatta caaatgatac atttaaagat
     5881 agaaggtatc aaatttaaaa attctttcag tatgtatgtt ttattaataa gtccgctggt
     5941 atttctttgt tttgctattt tcacagtctt attcgccaaa agtaatacgg gaacagcgaa
     6001 tagtgtgtca ccatatataa ctttactatt taatatttgg ccaattgctt tcatcccgat
     6061 tgtattatgt atggcttgta attcgttatt taaaattgaa atgagaaata aatcatttaa
     6121 ttattactta agtaataatt ggtcgattac aaaagaaata agagcaaaga ttttcatttt
     6181 atcaatagca tttttggtac attgcttt
//
LOCUS       DANRDI010000151         6150 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.152, whole genome shotgun sequence.
ACCESSION   DANRDI010000151 DANRDI010000000
VERSION     DANRDI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.152"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..77)
                     /locus_tag="PZ377_002313"
     CDS             complement(<1..77)
                     /locus_tag="PZ377_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359173.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIY"
     gene            complement(152..1162)
                     /gene="adhP"
                     /locus_tag="PZ377_002314"
     CDS             complement(152..1162)
                     /gene="adhP"
                     /locus_tag="PZ377_002314"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDM6359174.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGGKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(1661..2182)
                     /locus_tag="PZ377_002315"
     CDS             complement(1661..2182)
                     /locus_tag="PZ377_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDM6359175.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(2210..3505)
                     /locus_tag="PZ377_002316"
     CDS             complement(2210..3505)
                     /locus_tag="PZ377_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDM6359176.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(3545..4021)
                     /locus_tag="PZ377_002317"
     CDS             complement(3545..4021)
                     /locus_tag="PZ377_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6359177.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(4190..4726)
                     /locus_tag="PZ377_002318"
                     /pseudo
     CDS             complement(4190..4726)
                     /locus_tag="PZ377_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            complement(4937..5875)
                     /locus_tag="PZ377_002319"
     CDS             complement(4937..5875)
                     /locus_tag="PZ377_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDM6359178.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT         1958 a         1198 c          803 g         2191 t
ORIGIN      
        1 tcatatatgt aagccatttt ataaaataca aacaacacct caaaaccaaa aaacacgaga
       61 ttttcactat ctttcatgaa aacctcgtgt ttgattataa tgatagtgtt cagagcacta
      121 ccttctttat ctatatagtc agttgatatt tttaaaattt aataaccatt ctaccagtta
      181 tagtaccatt ttccatttct tcaaaaatat cattgatttc ttctaatttt cttaattgaa
      241 ctttaggtgt tactttattt tcagcagcaa attcaaacgc ttcacgtaag tcttgtcttg
      301 taccaacaag tgaacctact acttcaatac catcaagcac taatcttggg atatctaagt
      361 tcattttatc aacaggtaaa ccaacggcaa caactcttgc accagctttt acaacatcaa
      421 ccgcttggtt aaatggcgtc ttagcaactg aagttaccac tgttgcatct aatcctttgt
      481 tatcagttaa tttcataact tctgcaactg gatcaacatc tttagaatta ataatagcat
      541 cagcacctaa ttctttcgcg aatgctaatt tatcatcatt gatgtcgaag gcaacaattt
      601 taccccccat aacgttttta gcatattgta aagctaggtt acctaaacca cctataccaa
      661 aaacacctaa ccattgtcca ggttttacat tacttacttt tacagcttta taagttgtca
      721 cacctgcgca tgtaatagaa gacgctgctg ctggatctaa tttttcaggt actttcacag
      781 catagtctgc agtaacaata acttgttcag ccattgcacc atctactgta taaccagcat
      841 ttttcacact acggcaaagt gtttcacgac ctgttgtaca atattcacat cttccacagc
      901 tttcgaacat ccaagcgata gacacacggt ctccaatttt taatgattct acatcttccg
      961 caacttcgat gactttacca ataccttcat gacctaaagt aacgcctgta acatcaccaa
     1021 aatcagcatt cttaacatgt aaatcggtat gacaaacgcc acaatattcc gtttgtacca
     1081 acgcttcacc aggttttaaa gctcttaact ttttgtcctc aatacttact ttgtgatctt
     1141 tcgttacaac tgctgctctc ataataatgt cctccttata gtcactccag tatgtatgat
     1201 attaattggc tattactttc aaaaaagaaa acgaataatt atagatgtaa tttttatttg
     1261 taatagacgc cagattaaaa catactactg tgaccttgtt tttagttaga tatcaaccgt
     1321 taacttttaa cgtttcccta gttaatatgt taaaagaaat gacataatcg atatgctaac
     1381 gtgtaatttt tacttcacat atatttattt aaagataatt tataatactc ttatcttcac
     1441 tcttattata atgtgaatta attcacaaag tatatagcta tgatcaaaaa aatgtgtcag
     1501 ttattcaaat tttttattaa ctacagatta atttaatttc aaaaaaatta aaaaaactcc
     1561 caatcaatct aagacattat tttcacctca cgatttacgt cgtcattgag aaatgaatca
     1621 tatctttata gattaattgg gaaattttag tttctaatat ttaattaaat gcttgatgta
     1681 aagcatcaga agaatgcttc cacatacctt catcatggtt cattaaaaag tcttttaaaa
     1741 tagctttatt ctcgtcacca atttgatcta tgataatatg atgtttcatt gatttatcca
     1801 tcatattaac atgttcaggc atacttttat aaccacgacg aattgcttta ttcactaata
     1861 aatagcacgc tgttaaacca gcgtaatatg gtccttgttc attacgttct gttgttaccc
     1921 aacacaacca atattcttta gcttcatcgc cttcaacagc agctttatca gtaatccatt
     1981 tcacaccttt ttcaactttg gcacgagcat gcatgcctcc aatatcaata aatgcttctt
     2041 tgttagcaac atcgataaaa actggtgcaa tattgtctaa gctaattgaa ccaatatttg
     2101 tacctttatg accatctaga gggtcatttt ttatgatatt aaaattaaag ccttttttct
     2161 cagacaatga tataacctcc atatttaacg ataacaattt tagccctgtt taattaagct
     2221 cattaatcaa attttcaata gcttctctaa ttgtcttatc tttaatcttt gcaaccattt
     2281 ctctaggata atataattta tagtcttggc gattaatgcc tgtaatacta tgaatgacta
     2341 atgattgatt gcttatttct ctaatcgtac catcttgtct taataaatga atcggtttgc
     2401 gatttgaccc cggtcgatca tagtcatatg gcaaatcaga aaatgcttca ctcacaaaat
     2461 aataatctgg attaatacca cctgcttcaa acagttcttg cagttctgat attgtaataa
     2521 ttgagccatc aaatggaata tatttaaata agtctcgatt aataaatcga cttgctaaat
     2581 cacttaaaat agcatcatct tcttttgtcc atttttccaa ataatacgta actaccgctt
     2641 catcgagttc aacatattgt tcaatcgtaa ctgtctcttc aaaaaatgga ataaaatcat
     2701 gtggatgcaa cttaaattca tagccttcat tataaagctg ttttgcgcgt ttcaaacaat
     2761 tattaagcag cacttctcca ccacgactaa ctgggtggaa gtaaatttgc caatacattt
     2821 gataacgact cataataaag ttttcaactg catgcatacc actttctttg attagtactt
     2881 catctttaga aggtcgcatt aatcttaaaa tacgctccat atcaaaagca ccatatgata
     2941 cacctgtgaa atacgcatca cgttgtaaat aatccattct atccgcatcg atttgtgacg
     3001 aaatcatcga aatgaccaat ttattatgat gcgttttatt aattacttcc gcaacttctc
     3061 ttggaaactc aggcgccact ttacgtaata cagcattcac ctcagtatct ccagtaataa
     3121 tcgcttgtgt gtatgcttca tggtctgtat taaatatttt ttcaaaacta tgtgaaaatg
     3181 gaccatgtcc taaatcatgt aataatgcag cacataatgc caacggtcta tctttattgt
     3241 cccatgcatc atgaccaata aatgactcat caattaatcg tctaactatt tcatacacac
     3301 ctaaagaatg tccaaagcga ctatgttctg ctgtgtgaaa agataggtac agtgttccta
     3361 gttgtctaat tcgacgtaac ctttggaatt ccttagtttt aattaaatcc catatcaatt
     3421 gatcttcaac atgaatatat cgatgtatcg ggtctttaaa aactttttct tctactaatt
     3481 ttaaatctac atatgcgtta gtcattattc ccctcctttt cgtttaatat aatatttaat
     3541 ttacttaaaa tgctttgtac ataagtgcta agtctaactt ttcgccatac atttctggct
     3601 cataagagcg taagattgta aaaccttgct ctttatagta agctactgct tcttcatttt
     3661 tattatctac ttctaagtaa acaccttcaa atttatcttc aaaacgtgat aatccttcat
     3721 ttaacaatgc tgtaccataa cctgtatgtt gcgattctgg tttaacataa tgagctgata
     3781 aatataattc ttcaccgtaa ataaagttag caaagccaac gatgtcatta ccttcttcaa
     3841 cgactaagaa taattgttct tgaagtcttt tctttaaatg atgttcatta tatgaagctt
     3901 ctaacaagtg attaactgtt gtcgcagcgt atatatttaa gtatgtatta aaccaagctt
     3961 tagttgcgac atctctaatt tgaacaacat ctttttcagt tgcttgtctt accttgaaca
     4021 tgactttctc cccttattaa caagttttaa taacggcatt ataccacaac ttgctcaata
     4081 cttaataaac aatgattgtc tattcaattt atatatttat attttccgtt aaaattaaaa
     4141 ataaaaaata acgaagcaaa aaatcacttc gtttagtatg aggtatgtct tattgcaata
     4201 tactattcca ctcagttgca cgtgctaagg catagttgtc tttcatgatg tcaccaggct
     4261 tttcagcagt tccaataata taaccattta aagtggcacc taaaaagtct aaactatatt
     4321 tcatttgcgt aattgctggc ttcgctttta tttttggaca atctccacca actaaaataa
     4381 ctctaaaatc cttttcggcc atttgtgcct taaaattagg atatcgttta tcttgtaatg
     4441 tttctgacca atgttcgata aatgctttca atggtgctga aatgctatac caatacactg
     4501 gtgatgcaaa aataattgta tcactagcca atattttatc tagaatcggc aaatagtcat
     4561 cgtcatatga agtaatagtc tctgctgtat gtctcacgtc acgtatcggt ttaaactgat
     4621 gttgtgtcac gtcaatccat tgatactctt aatcttgcaa agcgaatttt gttaattgtg
     4681 cagtattacc gtttggtcta ctcccaccaa acaaaacagt aatcatttta gcctaacctc
     4741 acttttgatt aataaatatc tgtgtttttc gttacctaat tatactatca taagctttgc
     4801 ctaccgaata gtaaaacgct tacaactttt atataaattt gacgaaattt cgtcatgcct
     4861 tatataacgt cgtttgtgat acggggctaa ttcatgatga aattagatac atatatcacc
     4921 attaaataca attcatttag tcttcaatcg gaaacagttc atcgatatat tgaatctcat
     4981 catctgataa aacgatatct gcagctttaa tattttcaac gacttgttct gcacgttttg
     5041 caccaggaat aatcacatcg atagctggtc tcgttaaata aaatgctaat acaatgttcg
     5101 caattgaagt ttgatgtgct gcagctatgc tttccaaagc ttttacgcga cgcacatttt
     5161 cttcaaatac acctggttta aaatcacgac gtgtagtacg atggtcacta aatttagtgt
     5221 tctcatcata ttttccagct aaaataccgg atgctaatgg gaaatatgga ataaatgtga
     5281 tttggtgatc aacacaatat tgtaatactg cctcattttc gcgatgcaat aaattatatt
     5341 ctaactgtac aacatcaacg taaccatctt tatttgcttc tttaagttga tctaatgtga
     5401 aatttgatac accaattgct ttaatcttcc cttgttcctt aagctcttgt aatgctgcaa
     5461 ctgcttgatc tttcggagtg ttgttatccg gaaaatgaat ataatataaa tcgatataat
     5521 cagtttgtag acgtttcaaa ctattctcaa cttgttgttt taaatattcc ggttgattgt
     5581 tctgatgtac ttcttgattt tcatcaaatt catgagaccc tttcgtagca attttaattt
     5641 gctctcgcgg atattcttta acaacttctc caaccaattc ttctgatcgt tctggcccat
     5701 aaatatatgc cgtatctaat aaattaatac catgattaat ggcttgacga acaacatctt
     5761 ttccttgttc ttcatctaag ttcggatata aattatgccc acctactgcg ttcgtcccaa
     5821 gtgcgattgg aaacacttca acatcagatt tacctaagtt tacaaattgc ttcattagac
     5881 ccagctcctt atctttataa cttttccact atcacactat catgatttaa tacaacttcc
     5941 aaatgttaga attagagctt gaatttaagc ggtgtaatag gaaaacgtta tataaaactc
     6001 agttaaaata agtattgctt aaattgatat gcatatagcg cgattgctcg ttgaccttta
     6061 aattacatgt acttttgaat cagaaattga aaaacattta agaacgacaa tattaaagcc
     6121 attgcaaatt taatttggta atttctattg
//
LOCUS       DANRDI010000152         6087 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.153, whole genome shotgun sequence.
ACCESSION   DANRDI010000152 DANRDI010000000
VERSION     DANRDI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.153"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            89..1165
                     /locus_tag="PZ377_002320"
     CDS             89..1165
                     /locus_tag="PZ377_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDM6359179.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            1413..2075
                     /locus_tag="PZ377_002321"
     CDS             1413..2075
                     /locus_tag="PZ377_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDM6359180.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            2068..2844
                     /gene="fetB"
                     /locus_tag="PZ377_002322"
     CDS             2068..2844
                     /gene="fetB"
                     /locus_tag="PZ377_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDM6359181.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            3240..4427
                     /locus_tag="PZ377_002323"
     CDS             3240..4427
                     /locus_tag="PZ377_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6359182.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(4489..5841)
                     /locus_tag="PZ377_002324"
     CDS             complement(4489..5841)
                     /locus_tag="PZ377_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDM6359183.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIDPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
BASE COUNT         1961 a          895 c         1204 g         2027 t
ORIGIN      
        1 agcctgaaaa tgataaagcg gtagggaacg tttttctgaa agttagtgat acaatagttt
       61 taagttgaaa tacaggagga tgaataacat gaatcagtca gtcaaattac ttaaacattt
      121 aacagatgta aacggcattg ctggttatga aatgcaagtt aaagaagcaa tgcgtaacta
      181 tatagagcct gtcagtgatc aaattattga agataacttg ggtggcattt ttggaaagaa
      241 aaatgctgag aatggtcaat actcaattat gatttctggt catatggatg aagttggttt
      301 tatggtaaca aagattgata aacatggttt tatttcattt acgccagttg gtggatggtg
      361 gaatcaagtc atgctatctc aaaaagtaac gattacaaca gattcgggca aagaaattag
      421 aggtatcatc ggttctaaac cgccacatgt cttaacgcct gaagaacgta aaaagccaat
      481 ggaaatcaaa aatatgttta tagatattgg tgttagtagc aaggaagaag ctgaagaagc
      541 tggcgttgaa gtaggcaata tggttacgcc atatagtgaa tttgaagtgc ttgcaaatga
      601 taaatattta actgcgaaag catttgataa tcgctatggc tgtgcattag ctattgaggt
      661 attaaaacgt ttaaaagatg aaaatattgg cattaactta tacagtggtg ccacagtgca
      721 agaagaagtt ggtttgcgtg gtgcgaaagt ggcagcgaat acgattaaac cagacttggc
      781 gatagctgtc gatgtaggta ttgcttatga taccccaggt atgtcaggtc aaacgagcga
      841 tagtaaacta ggcggtggtc cagttgtcat tatgatggat gctacaagta ttgctcacca
      901 aggtttgcga aagcatatta aagatgtagc taaggaacat aacatcgaag tacaatggga
      961 tacgacacca ggtggaggta cagatgcggg aagtattcat gtcgcaaatg aaggtattcc
     1021 aacgatgaca atcggtgtta cgctgcgata catgcattct aatgtttcag tgctcaatgt
     1081 agatgattat gaaaattcta tccgtcttgt tactgaaatt gtccgttcat tgaatgatga
     1141 aagttataaa aatatcatgt ggtaatcaaa tccataaata ataaagaatc cttttaatat
     1201 ggtaggttgt taaacaattg tctaatttta attcttagtc attagacagt atccatgtta
     1261 ataggatttt ttgtttttaa tttaaatgct gaaaatcaat tatgcctaaa ttttgatatt
     1321 acaagaaaat gattttttct taaatgtaat tgcactaaaa accaaaaaaa cgggaataat
     1381 atacctgata tattacatga ggagcggtgc aaatgttgtt agaaattaaa gatttagtgt
     1441 ataaagcgag cgatagaatc atactagatc atatcagtct aaaagtagat aaaggcgaga
     1501 gtattgccat tataggtcca tcaggtagtg gtaaaagtac atttcaaaag caaatatgta
     1561 atttgtttag tccaactagt ggagaacttt attttaaagg taaaccctat aatgattatg
     1621 acccggaaga attgcgtcaa cgaatcagtt atttgatgca gcaaagtgac ttgtttggtg
     1681 aaacgattga agataacatg atattcccat cacttgcacg taatgataaa tttgatagaa
     1741 aacgtgcaaa gcaattaatt aaagatgtcg gtttgggaca ttatcaatta agttcggaag
     1801 tggaaaatat gtcgggtggt gagcggcaaa gaattgctat agcgcgccaa ctgatgtata
     1861 caccggatat tcttttatta gatgaatcga ccagtgcatt agacgttaat aataaagaaa
     1921 agatagaaaa tatcattttt aaattagcag atcaaggcgt ggcaattatg tggattaccc
     1981 acagcgatga ccaaagtatg cgacactttc aaaagcgtat aacaattgtt gatggtcaaa
     2041 tttctaatac agaggagttg aatcaacatg agtaatacgg cacttggatt gacggcattg
     2101 cttttagtca ttccgattat catttcatat aaagaaggtt tacatattat taaagattta
     2161 attgttgcga cattacgagc agttgtgcaa ttaatcattt tgggattttt gctgcattat
     2221 atttttaaaa taaacgataa atggctgctt attttatgtg tattggtcat tattattaat
     2281 gcatcatgga atacaattag tcgagcatca ccagtgatgc atcatgtgtt ttggatatca
     2341 tttctagcta tcttcattgg aacggcatta ccgcttgcag gtactattgc gacaggggcc
     2401 attcaattta ccgcaaatga agttatacct atcggcggca tgcttgcaaa taatggcttg
     2461 attgcaatta atttagctta ccagaattta gatcgtgcat tcgtacaaga tggtactaat
     2521 attgaatcta aattatcact tgcagctaca cctaaattgg cttctaaagg tgcaatacgt
     2581 gaaagtattc gtttagctat agtgccaact attgattcgg ttaaaacata tgggcttgtg
     2641 tcgattcctg gtatgatgac aggcttaatt attggtggcg taccaccttt acaagcgatt
     2701 aaatttcaat tgttagtcgt gtttattcat acaactgcga ccattatgtc tgctttgatt
     2761 gcgacatatt taagctatgg tcaatttttc aatgcaagac atcaattagt agcacgaaat
     2821 actgatgtta agagtgaatc atgatagatt ttactgcatc agatttaggc attagtttta
     2881 attggaaatg aagtgacgcg cacatatagt atcgctattc attagcgcag cgaaaatatt
     2941 cataaaggca cgcatacttt gtagtcagtt atctgttctg acatataaag cgtgcgtgct
     3001 tttttggagt tattgttgaa actgaagtaa ttatacataa ttattaaatg acatacttgt
     3061 gttaattttt caaatactga aaaacaattt caataatttt ccaattaagc acagaaaatt
     3121 aaagcaaaat attatataat agaacggtta tatataaaat tgtgttgcac acatttttta
     3181 ataaatcgtt attctaaggg aaatgaatat cggaaatttt gtttgaaagg agttttaaat
     3241 tgtcaatcat gcgactattt acattcattt taagtatttt tatcgtagga atggttgaaa
     3301 tgatggttgc aggaattatg aacttgatga gtcaggactt acatgtatca gaagctgtcg
     3361 ttggtcaatt agtgacaatg tacgctttaa catttgcgat atgtggacct attctggtta
     3421 aattaacgaa ccgtttttca tcaaggcctg tattattatg gacattactt atatttatca
     3481 ttggtaatgg cattattgct gtagcgccaa atttttcaat attagtagtt ggtagaatta
     3541 tctcatctgc agcagcagca ctaattatcg taaaagtatt agctattaca gcgatgttat
     3601 cagcacctaa aaatcgtggt aaaatgattg gacttgtcta tacagggttt agtggtgcta
     3661 atgtttttgg tgtaccaatt ggaacggtta tcggcgattt agtaggttgg cgctatacat
     3721 ttctattctt aattattgtg agtattattg ttggcttctt gatgatgatc tatttaccga
     3781 aggatcagga aatacaacga ggccctgtga atcatgagac accatctcat gaaaatcatg
     3841 ttacttcgaa aatattaaga cctgctgaag tagcaaaata tttaattatt acgtttttag
     3901 tattgattgc aaactcagtg acattcgtct ttataaatcc acttatttta tctaatggtc
     3961 atgatatgtc attcgtttca ttagcacttc tagtaaatgg aatcgctggc gttattggaa
     4021 catcattagg tggtatattc tccgataaaa ttacaagtaa gcgttggtta atgatttctg
     4081 tttctatttt tatcgtcatg atgttactta tgaatttaat cttacctggt tcaggtctat
     4141 tgttagcagg actatttatt tggaatatca tgcaatggag tactaatcca gcagtgcaaa
     4201 gcggtgtgat tcaacatgtt gaaggcgaca caagccaagt aatgagttgg aacatgtcta
     4261 gtttaaacgc tggtattggt gttggaggca ttattggagg cttggtcatg acacatgttt
     4321 ctgttcaagc tatcacatat acgagtgcca tcattggcgc attaggatta atcgttgttt
     4381 tcacattgaa aaataatcat tatgctaaaa catttaaatc atcataattc tcatatgaaa
     4441 agcacgcctg ctatcaaatt caggtgtgct tttttagatg cgataacgtt attgatatgt
     4501 gcgataatag cgacgttcat tatgatacat cggccaaggc attttaccgc ttttagcaaa
     4561 attagctaaa tcattttgca tttgtcgact taaaaattta aggtgagcag ttgttggata
     4621 ttgatgtgct gctaatattt gtaagtttcc gaaccaaaat accatatcta gaatatggta
     4681 agcactgcga tagtgtgcgc tagaagtatc atgccatgca aattcagcaa gccatagttg
     4741 tgcgtttgaa tcttgtatat tgagttgttg taaaaattgc ttcatcggct gtttgaaata
     4801 acaatgtgta atcgctaaag attgttgctt cgccgtctga acatcttcgt attttaaagg
     4861 aatatcattt aattccataa tgtcgataaa gcgttgcggc gataattttt tctgttcact
     4921 agtaatataa atatcgcctt catcttttgt ataacatgca aaaattggtt tcgttgttgg
     4981 ataattattt tgtatataat ctgttttaat aggcgcatat attaaatcaa gccctttaga
     5041 aggtcctcga gattgtttta gcttcgccat cagcataaga atatcatttg tcgataatga
     5101 tgtaacatca tctgtatcta aataatcgag catcattttt tggaaatgtt gtgctttatt
     5161 gcgtgcactc tcaagggtgt ctaatcgtag tgcgccactt agtagaacga ctttatggaa
     5221 gtatggatca atgtcaggta ttttaagtaa agtcaaaatg ctcatactgc ctgcagactg
     5281 acccattaaa gtaatgttat tagcgtcgcc accgaaggat tcaataaatt gatgcaccca
     5341 ttttatgaca ttgatttgat ctgaaaggcc attattggaa tgaaaatctt tattaaaata
     5401 tgaccagtct aaatatccta atgcgcctaa acgataattg catgtaataa cgataatgtc
     5461 gttattttgt actaaatgtg ccggttgata gagttcggct gtaccatgac cattttcaaa
     5521 actaccacca taaaaataaa tgatgacagg tttcttcgtc tgatcattat gttgtttcca
     5581 aatatttaga tataaacagt cttcatgttc agtaaaagtt gtagattgtg aggagaaaaa
     5641 atcttctaat ttgttgtctg gttgcggtgg gatgggttgt atttcagttg catcaattgg
     5701 ctctgaccat tgtgttttta acgtagaatg tttaaagcga ttgtcatgaa ctggtggttc
     5761 tgcataagga atgcctaaga agatatctaa accatcttgt gtaataccat gaatttgacc
     5821 acctgtagta ttaatcttca tatttattaa cctcctttat catccccaat ttaatcatac
     5881 cacggtctat agatgatagg tgacaagtat aacaagacaa ttttgtgact gaatcagacg
     5941 agcttgatat ctgttttaac tcaaaccatt ttcagcatta tatgatgtac ttctacggga
     6001 gtgggacaga aatgataaag agccacaaat gatttattat gtagtggttc ttatacatta
     6061 gccacagcta atgtgtactt aaaaata
//
LOCUS       DANRDI010000153         5880 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.154, whole genome shotgun sequence.
ACCESSION   DANRDI010000153 DANRDI010000000
VERSION     DANRDI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.154"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..715
                     /locus_tag="PZ377_002325"
     CDS             <1..715
                     /locus_tag="PZ377_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDM6359184.1"
                     /translation="NGNNHVSTSKINKVLGVKNDSRMYTFSKKNAINDLEENPLIKSV
                     EIHKQLPNTLNVDITENEIIALVKYKGKYLPLLENGKLLKGSNDVKINDAPVMDGFKG
                     TKEDDMIKALSEMTPEVRRYIAEVTYAPSKNKQSRIELFTTDGLQVIGDISTISKKMK
                     YYPQMSQSLSRDSSGKLKTRGYIDLSVGASFIPYRGNTSSQSESDKNVTKSSQEENQA
                     KEELQSVLNKINKQSSKNN"
     gene            821..2233
                     /gene="ftsA"
                     /locus_tag="PZ377_002326"
     CDS             821..2233
                     /gene="ftsA"
                     /locus_tag="PZ377_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDM6359185.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            2266..3438
                     /gene="ftsZ"
                     /locus_tag="PZ377_002327"
     CDS             2266..3438
                     /gene="ftsZ"
                     /locus_tag="PZ377_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDM6359186.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            3698..4489
                     /gene="pgeF"
                     /locus_tag="PZ377_002328"
     CDS             3698..4489
                     /gene="pgeF"
                     /locus_tag="PZ377_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDM6359187.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            4513..5181
                     /locus_tag="PZ377_002329"
     CDS             4513..5181
                     /locus_tag="PZ377_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDM6359188.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            5178..5741
                     /locus_tag="PZ377_002330"
     CDS             5178..5741
                     /locus_tag="PZ377_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDM6359189.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            5753..>5880
                     /locus_tag="PZ377_002331"
     CDS             5753..>5880
                     /locus_tag="PZ377_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDM6359190.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVG"
BASE COUNT         2199 a          813 c         1132 g         1736 t
ORIGIN      
        1 aaatggaaat aatcacgtta gtacttcaaa gataaacaaa gttttaggtg ttaaaaatga
       61 ttcaaggatg tatacgttta gtaaaaaaaa tgctattaat gatctcgaag agaatccatt
      121 aatcaaaagt gttgagatac acaagcaatt accaaacaca ttaaacgtag atatcacaga
      181 aaatgaaatt attgctttag tgaaatataa aggtaaatat ttacctttat tagaaaatgg
      241 taaattgctt aaaggttcaa atgatgtcaa aattaatgat gcacctgtca tggatggttt
      301 caaaggtaca aaagaagatg atatgattaa ggcgttatct gaaatgacac ctgaagttag
      361 acgatatatt gccgaagtga catacgcccc aagtaaaaac aaacaaagca gaattgaatt
      421 gtttacgaca gatggacttc aagtaatcgg tgatatttcg acgatatcta agaaaatgaa
      481 atattatccg cagatgtcac aatcattatc aagggatagt tcgggtaaac taaaaacacg
      541 aggctatatt gatttatcag tcggtgcttc atttatccca taccgtggaa acacgtctag
      601 tcaatcagaa agcgataaaa atgtgactaa atcatctcaa gaggaaaatc aagcaaaaga
      661 agaattacaa agcgttttaa acaaaattaa caaacaatca agtaagaata attaaaaaat
      721 tttgatattg tctatgttta tagttcacaa gccattcaac gtattgtaaa ctaaggatag
      781 tgtatttttt taatagtaat ttgtcaggag gtgcctatct atggaagaac attactacgt
      841 aagtattgat attggatcat caagcgtaaa aacaatagta ggcgagaaat ttcacaatgg
      901 tataaatgtg ataggtacag gacaaaccta cacgagcggt ataaaaaatg gtttaattga
      961 tgattttgat attgcgcgac aagcaatcaa agacacaatt aaaaaggcat caatcgcttc
     1021 gggtgttgat attaaagaag ttttcctgaa attacctatc attggaacgg aagtttatga
     1081 tgaatcaaat gaaatcgact tttatgagga tacagaaatc aacggttcac atatcgaaaa
     1141 agtattagaa ggtattagag aaaaaaatga tgtgcaagaa acagaagtaa ttaatgtgtt
     1201 cccgattcgt tttatagtcg ataaagaaaa tgaggtttca gaccctaaag aattaattgc
     1261 cagacattca ttaaaggttg aagcaggcgt aattgctatt caaaaatcga ttttaattaa
     1321 tatgattaaa tgcgtagaag catgtggtgt tgatgtatta gatgtttact ctgatgcata
     1381 taactatggt tcaatcctaa cagctactga aaaagagtta ggtgcatgtg tcattgatat
     1441 tggtgaagac gttacgcaag ttgcttttta tgaacgcggt gaattagtag atgctgattc
     1501 tatcgaaatg gcagggcgtg atattacaga cgatattgca caaggattaa acacttctta
     1561 tgaaactgct gaaaaagtta aacaccaata tggtcatgca ttctatgatt ctgcttcaga
     1621 tcaagatatc ttcactgttg aacaggttga tagtgatgaa acagtacagt atactcaaaa
     1681 agatttgagt gactttattg aagcgcgtgt agaagaaata ttcttcgaag tatttgatgt
     1741 tttacaagat ttaggattaa caaaagtaaa tggtgggttt attgtaactg gtggatctgc
     1801 aaacttactt ggcgtaaaag aattattatc agatatggta agtgaaaaag ttagaattca
     1861 cacgccatca caaatgggaa ttagaaaacc tgaattttct tcagcaattt ctacaatttc
     1921 tagtagtatc gcttttgatg agttattaga ttatgttaca attaattatc atgataatga
     1981 agaaactgaa gaagatgtta ttgatgtgaa agacaaagat aacgaatcta aattaggcgg
     2041 atttgattgg tttaaacgta aaacaaacaa aaaagatact catgaaaatg aagtagagtc
     2101 aacagatgaa gaaatttatc aatcagaaga taatcatcag gaacataaac agaatcatga
     2161 acatgttcaa gacaaagata aagataaaga agaaagtaaa ttcaaaaaac taatgaaatc
     2221 tctatttgaa tgattattgg ccaataaaac taggaggaaa tttaaatgtt agaatttgaa
     2281 caaggattta atcatttagc gactttaaag gtcattggtg taggtggtgg cggtaacaac
     2341 gccgtaaacc gaatgattga ccacggaatg aataatgttg aatttatcgc tatcaacaca
     2401 gacggtcaag ctttaaactt atctaaagct gaatctaaaa tccaaatcgg tgaaaaatta
     2461 acacgtggtt taggagcagg agctaatcct gaaatcggta aaaaagctgc agaggaatct
     2521 cgtgaacaaa ttgaagatgc aatccaaggt gcagacatgg tatttgttac ttctggtatg
     2581 ggtggcggaa ctggtactgg tgcagcacca gtcgttgcta aaattgcaaa agaaatgggc
     2641 gcattaactg ttggtgttgt aactcgtcca tttagttttg aaggacgtaa acgtcaaact
     2701 caagctgctg ctggagtaga agctatgaaa gctgcagtag atacattaat cgttatacca
     2761 aatgaccgtt tattagatat cgttgacaaa tctacgccaa tgatggaagc atttaaagaa
     2821 gctgacaacg tgttacgcca aggtgtacaa ggtatctcag acttaatcgc tgtttctggt
     2881 gaagtaaact tagactttgc agacgttaag acaattatgt ctaaccaagg ttctgcatta
     2941 atgggtattg gtgtttcttc tggtgaaaat agagcggtag aagctgctaa aaaagcaatc
     3001 tcttctccat tacttgaaac atctatcgtt ggtgcacaag gtgtgcttat gaatattact
     3061 ggtggcgagt cattgtcatt atttgaagca caagaggctg ctgatattgt ccaagatgct
     3121 gcagatgaag acgttaatat gattttcggt acagttatta atcctgaatt acaagatgag
     3181 attgttgtaa cagttattgc aactggtttt gatgacaaac caacatcaca tggtcgtaaa
     3241 tctggtagca ctggattcgg aacaagcgta aatacttcta gcaatgcaac ttctaaagat
     3301 gaatcattca cttcaaattc atcaaatgca caagcaactg atagtgtaag tgaaagaaca
     3361 catacaacta aagaagatga tattcctagc ttcattagaa atagagaaga aagacgttca
     3421 agaagaacaa gacgttaatc ggttaatata tatacacaaa taattcaaca caaatcatca
     3481 gataacatat ctgatgattt ttttactaat ttttagaaca tgtagaagga catttaagtt
     3541 tttcaaagtt attaaaagtg tttaagtatc gtgtgaaaat taagtcaaaa attatttgcg
     3601 caacatttta actttaaaca taaatgttat attatataat tattaacttt gtacagttag
     3661 acgaagataa tttaaatgaa atgatggtga cgatcgagtg aatgataatt ttaaaaagca
     3721 accgcatcat ttaatatatg aagagttatt acaacaaggt attactctag gtattacaac
     3781 tagaggagat ggtttaagtg actatcctaa aaatgctttt aatatggcga gatatattga
     3841 tgatcgccca tataatatta ctcaacatca attgcaatta gctgaagaaa ttgcgtttga
     3901 tagaaaaaat tgggtgtttc ccattcaaac acatgaaaat aaagtcgctt gtattacaaa
     3961 ggatgatata ggcacaaata tagacacttt aactgatgcg cttcatggta ttgatgcgat
     4021 gtacacatat gatagtaatg tcttattaac gatgtgttat gcagactgtg taccagtata
     4081 tttttatagt acaaaacatc attttattgc attggcgcat gcaggttggc gtggtaccta
     4141 tactgaaatt gtaaaagaag tgctaaaaca tgtgaacttt gatttgaaag acttacatgt
     4201 cgttattgga ccatctacat catcaagtta tgaaattaat gatgatatta aaaataaatt
     4261 tgaaacattg ccaattgata gtgccaacta tattgaaact agaggacgag atcgtcatgg
     4321 tattgatttg aaaaaagcca atgctgcatt attaatttat tatggtgttc ctaaagaaaa
     4381 tatttatacg acagcgtatg ctacatctga acatttagaa ttatttttct cttatcgatt
     4441 agaaaaaggt caaacaggac gcatgttagc attcattggt caacagtaaa caaggaggag
     4501 atatgtttgc gtgtgaaaga taatttacaa caaatctcaa cacaaattaa tgacaaaagt
     4561 gaaaaaaata atttttcaac aaaaccaaac gtgattgcag ttacaaaata tgttacaata
     4621 gagcgagcta aagaagcgta tgaggctgga ataagacatt ttggtgagaa tagattggaa
     4681 ggcttttttc aaaagaaaga agcattacca tcagatgcgg tgatccattt tataggatca
     4741 ttacaatctc gaaaagttaa ggacgttata aacgacgtag attatttcca tgctttagat
     4801 cgattgagct tagccaaaga aattaacaaa cgtgcagaac ataaaattaa atgtttcttg
     4861 caagtgaacg tttcgggaga agcttctaaa catggtattg ctttagaaga tgttgatcag
     4921 tttatagatg atcttaaaaa atatgacaaa atcgaaattg taggtttaat gacgatggca
     4981 ccattgacag atgatgaagc atatattaga tcgttattta aacagttacg tttgaaaaaa
     5041 gaagaaatac aacgactcaa tttagaatat gcgccttgtg atgaattatc aatgggaatg
     5101 agtaatgact atcttattgc agttgaagaa ggtgcgacgt ttgttagaat tgggactaaa
     5161 cttgtaggag aagaggagtg agccacttgg ctttaaaaga tttatttagt ggattttttg
     5221 taatagatga tgaagaggaa gtagaagtac ctgacaaaca acaacaggta aatgaagcgc
     5281 cagcaaaaga gcagtcacaa caaacaacaa aacaaaacgc aatcaaatca gtccctcaaa
     5341 aatctgcatc aagatataca acaacgtcag aagaaaggaa taaccgtatg tctaattatt
     5401 caaaaaataa ttcacgtaat gttgtaacta tgaacaatgc tacaccaaac aatgcatcac
     5461 aagaaagttc aaaaatgtgt ttattcgaac cacgtgtttt ttcagataca caagatattg
     5521 ctgatgagct taaaaaccgc cgtgcgacac ttgtcaattt acaacgtatt gataaagtat
     5581 cagcgaaaag aattattgat tttttaagcg gtactgttta tgcaatcggt ggagatatcc
     5641 aacgtgtagg tactgatatt ttcttatgta cgcctgataa tgtggaagta gctggaagca
     5701 ttacagacca tattgaaaat atggaacatt cattcgacta aggagttaac atatggatat
     5761 aaatgtgcta gctacaatat ttaaattcat cctttttgtt gttgaaattt attatttcgg
     5821 catgattata tatttcttta catcttgggt accaagtatt agagaaacta aggtaggtta
//
LOCUS       DANRDI010000154         5809 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.155, whole genome shotgun sequence.
ACCESSION   DANRDI010000154 DANRDI010000000
VERSION     DANRDI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.155"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            100..300
                     /locus_tag="PZ377_002332"
     CDS             100..300
                     /locus_tag="PZ377_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDM6359191.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            588..2867
                     /locus_tag="PZ377_002333"
     CDS             588..2867
                     /locus_tag="PZ377_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HDM6359192.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLLDPDKILNEYVDWTDTLEWPT
                     SSKEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHE
                     GSTAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLP
                     SYKAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIP
                     NIEIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIY
                     DIHHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTT
                     TKKALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAY
                     EALQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTN
                     KVDFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPE
                     LEEEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNV
                     DYVERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFG
                     KDYGVQIAYVRDESLEDLI"
     gene            complement(2976..3074)
                     /locus_tag="PZ377_002334"
     CDS             complement(2976..3074)
                     /locus_tag="PZ377_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359193.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            3208..3498
                     /locus_tag="PZ377_002335"
     CDS             3208..3498
                     /locus_tag="PZ377_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDM6359194.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            3479..4846
                     /locus_tag="PZ377_002336"
     CDS             3479..4846
                     /locus_tag="PZ377_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDM6359195.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            4859..5296
                     /locus_tag="PZ377_002337"
     CDS             4859..5296
                     /locus_tag="PZ377_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDM6359196.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            5453..5767
                     /locus_tag="PZ377_002338"
     CDS             5453..5767
                     /locus_tag="PZ377_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDM6359197.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
BASE COUNT         2214 a          766 c         1139 g         1690 t
ORIGIN      
        1 acgaaaaaat cagagctgaa gtttcatggt aatagctatt atcatttttg aattaattat
       61 attaatgtgt ttagcaatag cactggaggt gttgtaaata tgtggattgt catttcaatc
      121 gttttagcta tatttttatt gatcttgtta agtagcattt ctcataagat gaaaaccata
      181 gaagcattgg agtatatgaa ttcttatctt ttcaagcagt tagtaaaaaa taatggtgtt
      241 gaaggtttag aagattatga aaatgaagtt gaacgaatta gaaaaagatt caaaagctaa
      301 agagggggct aaagccctct ccaggattaa aagaagcgga tattttttat ttcgtttttg
      361 taaattaaaa ctaatttatg tgaaattgtc tttgaagtct tgataattaa gaaggttgaa
      421 ttttctgaga ttatattagt tacgggaaag gcttttcctg agtgcaataa aattaaagtt
      481 ctcaagtttt cattttcgta tttatcataa atgatttcgc gtaaagtttt caaaatttgt
      541 cacctcttta attttattaa attaattata caagaaagga gccgaatatg ttagacaaag
      601 tcactcaaat agaaacaatt aaatatgatc gtgatgtctc atattcttat gctgctagtc
      661 gtttatccac atattggact aatcacaata tggcttggtc tgactttatg cagaagctag
      721 cacaaacagt tagaactaaa gaagatttaa ctgagtacaa taaaatgtct aagtctgaac
      781 aagcagatat aaaagatgtt ggcggatttg tcggtggata tttaaaagaa ggcaaacgac
      841 gtgctggtca agtcatgaat cgttcaatgc taacacttga tatcgattat gctgctcaag
      901 atatgactga catattatct atgttttatg attttgcata ttgtttatat tcaacacata
      961 agcatagaga gataagtcca agactgcgtt tagtgattcc tttgttagat ccagataaaa
     1021 tattaaatga atatgttgat tggactgaca cattagaatg gccaacgtct tcaaaggaag
     1081 agagtaagac taaaagatta gcagataagc aaggtgaccc agaagaaaag ccgggaattg
     1141 ttggcgcatt ttgtagagcc tatacgatag aagaagctat atcaactttt attcctgact
     1201 tatacgaaaa acattctact aaccgttata cctatcatga aggttcaact gcaggtggat
     1261 tggtgttata cgaaaataac aagtttgcct attctcatca taatacggat cccgttagcg
     1321 gtatgcttgt gaacagtttt gatttagtac gcatacactt atatggtgct caagatgaag
     1381 acgctaaaac agatactccg gttaatcgac tacctagtta taaagcaatg cagcaaagag
     1441 cgcaaaatga tgaagttgtt aaaaagcaat taattaacga caaaatgtct gatgcaatgc
     1501 aggatttcga tgaaatagta aatagcgatg atgcatggtc tgagacgtta gaaattactt
     1561 cgaaaggtac tttcaaagct agtatcccaa atatagaaat tatattgcgt aatgatccaa
     1621 atttaaaagg aaaaatagca tttaatgaat ttacaaaaca aattgaatgc ttagggaaag
     1681 tgccatggaa tactaatttt aagacacgtc aatggcaaga cggtgatgat agcagtttaa
     1741 gaagttatat cgaaaagatt tatgacatac accattcagg taaaacaaaa gatgccatta
     1801 taagcgtagc aatgcaaaat gcttatcatc cagtaaggga ttatctaaat aaaatatcgt
     1861 gggatggaca taaacgtctt gaaaagttat ttatcaaata cttaggtgtt gaagatactg
     1921 aagtgaatag aacaactacc aaaaaagcat tgactgctgg aattgctcga gtaatggagc
     1981 ctggatgtaa atttgactat atgcttacac tttatggtcc tcaaggtgta ggtaaatctg
     2041 ctttgctaaa aaaattaggt ggtgcatggt tttctgacag tttagtttct gttacaggta
     2101 aagaagccta tgaggcctta caaggcgttt ggctaatgga aatggcagaa cttgcagcta
     2161 caagaaaagc tgaagttgaa gctattaagc atttcatatc taaacaagtt gaccggtttc
     2221 gtgttgctta tggacattat attgaagatt ttccaaggca atgtattttc attggtacaa
     2281 ctaataaagt tgatttctta agagatgaaa ctggtggaag acgtttttgg ccaatgactg
     2341 taaatccaga gagagttgaa gtgaactggt ctaaactaac caaagaagag atcgaccaaa
     2401 tttgggcaga agctaaatat tattatgaac aaggagaaga gttattcctc aaccctgaac
     2461 tagaagaaga aatgcgttca atacaaagca aacatactga ggaatctcca tatacaggca
     2521 ttattgatga atatcttaac acaccaattc ctagcaattg ggatgactta actatctttg
     2581 aacgaagacg attttatcaa ggtgatgttg atatgttacc aacaggaaat gtagattacg
     2641 ttgaaagaaa taaggtctgt gcgcttgaag tgtttgttga atgttttggt aaagataagg
     2701 gagatagtag aggatctatg gaaattagaa agatttcaaa catcttaaga caattagaca
     2761 attggtctgt atatgatggt aataaaagtg ggaaaattcg atttggaaaa gattatggtg
     2821 tacagatagc ttatgtaaga gatgaaagtt tagaagattt aatataataa atattgaata
     2881 aatatacatt ttagagtgtt gtatcagatg ttgcatcatt ttttgagtga tgcaacacgt
     2941 gagtgtaaaa agtaatcgta ggtgttgcat catttttagt gatgcaacat tgatgcaaca
     3001 aatgatacaa cacctctttc ccttctcgct gtaaggttca accctgtttg tttccaatgt
     3061 tgcatcaaat tcactataaa gtttaaaaag tagtgttagg gagtaaagag gtataggggt
     3121 aaccctctaa cagctatttt taaaagtttg gcaagaattg atgcaacatc ggaacacaaa
     3181 tataaatttt gtatacaagg tgaatatatg aaagaatcga cattagaaaa atatttagtg
     3241 aaagagatat caaagctaaa cggtttatgt ttaaaatggg ttgcacctgg aacaagaggt
     3301 gtgccagata gaattattat tatgccagaa ggaaaaacat attttgtaga aatgaagcaa
     3361 gaaaaaggaa agttgcatcc tttacaaaaa tatgtgcata gacaatttga aaatagagat
     3421 cataaagtat atgtgttatg gaataaagaa caagtaaata cttttatcag aatggtaggt
     3481 ggaacatttg gcgattgact tcaaaccaca tagctatcaa aagtatgcaa tagataaagt
     3541 gatagataat gagaaatacg gtctgttttt agatatgggt ctagggaaaa cagtatcaac
     3601 acttacagca tttagtgaat tgcagttgtt agacactaaa aaaatgttag ttatagcacc
     3661 taaacaagtt gctaaagata catgggttga tgaagttgat aagtggaacc atttaaatca
     3721 tctgaaagtg tctttagttt taggaacacc taaagaaaga aatgatgcat taaacacaga
     3781 ggctgatatc tatgtaacca ataaagaaaa tactaaatgg ttatgtgatc aatataaaaa
     3841 agaatggcca tttgacatgg ttgtgattga tgaactgtct acatttaaaa gtcctaagag
     3901 tcaaaggttt aaatctatta aaaagaaatt accactcatt aatagattta taggattaac
     3961 aggaacacct agtccaaata gtttacagga tttatgggct caagtttatt tgatagacag
     4021 aggtgaaaga cttgagtctt cattcagtcg ttatcgagaa aggtacttta aaccaactca
     4081 tcaagttagc gaacatattt ttaagtggga gctaagagac ggatctgaag aaaagatata
     4141 taaacaaata gaagatatat gtttaagcat gaaagcgaaa gattatctgg atatgcctga
     4201 cagagttgat actaaacaaa cagtagtctt atcagaaaaa gaaagaaaag tatatgaaga
     4261 attagaaaaa aactatattt tagaatcgga agaagaagga acagttgtag ctcaaaatgg
     4321 ggcatcatta agtcagaaac tacttcaact atctaacggc gcagtttata cagatgagga
     4381 agatgtaaga cttatacatg ataagaagtt agataagtta gaggaaatta tagaggagtc
     4441 tcaaggccaa ccaatactat tgttttataa cttcaaacac gataaagaaa gaatacttca
     4501 aaggtttaag gaagcaacca cattagacga ttcaaactat aaagaacgtt ggaacagtgg
     4561 agacattaag ttgcttatag cacatccagc aagtgcagga catggattaa acttacaaca
     4621 aggtgggcac attattgttt ggtttggact tacatggtcc ttggaattat accaacaagc
     4681 aaatgctaga ttatacagac aaggacaaaa tcatacgact attattcatc atatcatgac
     4741 cgataacaca atagatcaaa gagtatataa agctttacaa aataaagaac taacgcaaga
     4801 agaattaatg aaagctatta aagcaagaat agctaagcat aagtaatgga ggtctaacat
     4861 ggggaacaca atatatgata tcaagccagg cacatttaaa tatattgaat cagaaatata
     4921 taatttaaat gagaacaaga aagaaataaa aagattgagg ttggaaatac ttaatccaac
     4981 gaaggaacaa gattccaata ttgtatatgg accattacaa aaaggcgaac cagttagaac
     5041 aactgaacta atggcaacta gattattaac taataagatg ttacgaaacc ttgaagaaat
     5101 ggtcgaagca gttgagagtg aatacttaaa gttgcctgaa gatcataaga aagtaataag
     5161 gctcaagtat tggaataaag aaaagaagtt aaagatggaa cagataggac atgaatgcca
     5221 tatgcatcgt aatactgtta ctactatacg aaagaacttt gttaaagcgg tagcgtatca
     5281 tgcaggtatc aaataacatt gtgcaaagat tgtgcaaaag gcctacaaat ctgtagtaat
     5341 atgatagtat cggaaagatg tataaagtta tctaaaagtt atacgacaca agtacacgag
     5401 gcacatcgct atgcggtgtg tcttttgtta tgcaatcaaa gaggtgtaag agatgaccaa
     5461 gcacaataac atttataagc atggtcgtaa gtcatatcaa tacgattggt tctatcattc
     5521 aaaagcatgg aagaagttaa gggagatagc attagataga gataatcatc tttgtcaaat
     5581 gtgtttacgc gaagatattg taacagatgc aaacatagtg catcacatta tttatgttga
     5641 tgaagatttt aacaaagctt tagaattaga taatctaatg tcagtttgtt atggctgtca
     5701 taacaaaatt catgcaaatg gtaatgacaa aagtaatctt aagaaaatta gagttctaaa
     5761 aatttaaata aaaaaacatt taaataaaat tttatagccc cctgcccat
//
LOCUS       DANRDI010000155         5789 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.156, whole genome shotgun sequence.
ACCESSION   DANRDI010000155 DANRDI010000000
VERSION     DANRDI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.156"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            100..783
                     /locus_tag="PZ377_002339"
     CDS             100..783
                     /locus_tag="PZ377_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDM6359198.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(899..1561)
                     /locus_tag="PZ377_002340"
     CDS             complement(899..1561)
                     /locus_tag="PZ377_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDM6359199.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(1554..2171)
                     /locus_tag="PZ377_002341"
     CDS             complement(1554..2171)
                     /locus_tag="PZ377_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDM6359200.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            2238..2708
                     /locus_tag="PZ377_002342"
     CDS             2238..2708
                     /locus_tag="PZ377_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDM6359201.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2855..3724
                     /locus_tag="PZ377_002343"
     CDS             2855..3724
                     /locus_tag="PZ377_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDM6359202.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            3745..4311
                     /locus_tag="PZ377_002344"
     CDS             3745..4311
                     /locus_tag="PZ377_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDM6359203.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            4742..>5789
                     /locus_tag="PZ377_002345"
     CDS             4742..>5789
                     /locus_tag="PZ377_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HDM6359204.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNY"
BASE COUNT         2214 a          812 c          969 g         1794 t
ORIGIN      
        1 atatttaaat agaacaatac tacgagttca ttttcagggg ataacgaata tcatagaatc
       61 agcataatac gaaataaata aataggagta ttgatatcaa tggaatggat attatttgat
      121 aaagatggta cgttaattga atttgataga agttgggaaa aaataggggt acgatttgta
      181 caatcattgc ttgagacttt cccagtacat aataaagaag ctgctttaag acaactcggt
      241 gtcattaaag aatctattga tccaaaatca gtgatgggtt caggatcttt acaacaaatt
      301 atccaggcat ttaatgatgt gacgggacaa gatacaaccg actggtccaa gtcaacaagt
      361 caaaagctgg tagatgaacg tattcctgaa attaattggg tagaaggtgt taaagaagca
      421 cttatcgatt tgaaagcaaa aggctatcaa cttggtattg ttacgagtga tactaaaaaa
      481 ggtgtagaac aatttttagc acataccaat gctacctcgt tgttcgattt gatcatttct
      541 accgaagcgg atgcctatga gaagccaaat cctaaagtat tatcgccttt atttgagcaa
      601 tataatgtag atcctcagaa agtagctata gtaggagaca ctgctaatga tatgaagaca
      661 gcaagtaatg caaatttagg tatggcaata ggtgtattaa caggtattgc aacaaaagaa
      721 gaattacatg aagctgatat tattttaaat agtgcggcag atattttaga agctttaaat
      781 taaaaagaaa gacatagtgt atatgtgaat taatgtattc gatagaattg ataaaataat
      841 cgataatatc tatggaatat acattgaata taaacatata cgctatgtct ttagtctttt
      901 atcgtgtatc tacttgtcga tatgtttgaa taattcgagc aattttgttt atcataggat
      961 ttaaagattc ggggtcctta tggatatcat attcattaat attgatacgt acaactggac
     1021 atgcattaaa gctattaatc caatcgtcat agcgtttaaa tagctttttc cagtattcag
     1081 ggtctgtatt aatttccatt tcgcgaccac gttcaataat acgatcaatg acctcatcat
     1141 agttacattc taaataaatc attacatcag gtttaggaaa ataaggtgtc atgaccatgg
     1201 cattaaataa gtctgaatat gttttgaaat cttctttact cattgtgcct tcttcttcat
     1261 gcatttttgc aaaaatatca acatcttcat aaattgatcg atcttggaca aagccaccac
     1321 catattcaaa catacgcttt tgttctttaa aacgttcagc taagaagtaa atttgcaaat
     1381 ggaaactcca tcgttcaaaa tcgctgtaaa atttatctaa atatggatta tgttcgacat
     1441 tttcaaaaga cgttttaaag tttaatttat ctgcaagtgc ttgcgttagt gttgattttc
     1501 caacaccaac tgtacctgca atggttataa tggcattttg tggaataccg taattattca
     1561 ttggtaatat ctcctatcat aggtaatata atatgtaata tatcttcgta atcttgttca
     1621 tttttaagaa aatcaataga agttgtatcg attaaaacta catttgaacc attactttgt
     1681 aaggactcat aatactcacg ataatctttt tttaacttta acagatattc atcttctatt
     1741 tgatgctcaa aactacggtt acgtttagca attctagatt ttaacacatc aaggtctgca
     1801 tctaaaaaga taatcatatt cggcataatc atatcttcag ttaaaatatc ataaatttta
     1861 ctgaatttct gaaattcaac agaactcaaa gtatttttag caaatatctt atttttatgt
     1921 atatgataat cactaactac accttgattt agttgtgtta catcttgaaa ttgcttatat
     1981 ctattgcata aaaagaacat ttcagtttga aaactccatt tagagatatc ttcataaaag
     2041 tctgataaaa atggattttc tgtgatgatt tctttttctt cataaaaatc taaagtttga
     2101 cttaatttgt gtgcaagtga agatttacct acgccaatag gaccttcaat tgctataaaa
     2161 ggtttgttca tattcacatc tccaaatgtt aaaatagaca ttgagtattg taccataagt
     2221 gaggtatctg gacataaatg acaaatgata tatattttat gacattagcg attgaagaag
     2281 ctaaaaaagc agctcaacta ggcgaagtac ctataggtgc tatcatcact aaagatgatg
     2341 aagttatcgc tagagcacat aatttaagag aaacactaca acaaccaacg gcgcatgctg
     2401 aacatattgc aattgaacgt gcagccaaag tgttaggtag ttggcgttta gaaggttgca
     2461 cattatatgt aaccttagaa ccatgtgtca tgtgcgcagg aacaattgta atgagtcgca
     2521 ttccaagagt cgtctatggc gcagatgatc ctaaaggtgg ttgtagtggc agtttaatga
     2581 atttattgca acaatctaat tttaatcatc gtgcaattgt tgataaaggt gtacttaaag
     2641 aagcatgtag cacattatta acaacatttt ttaaaaactt aagagccaat aagaaatcca
     2701 ccaattagac gatagtacat actaattaaa atttgttaga attacattat atgataaatt
     2761 aatgacaata taaatgttaa tagaaaagta tgtgcgctga gtatatattc ttatttaaga
     2821 acaagatttt aaatttacga aacgaggtaa cataatgata aaactaatag ccactgatat
     2881 ggatggcacg ctacttaatg cagcacatga aatttctcaa cctaatattg atgcgattaa
     2941 atacgctcaa gaacaaggga taacggttgt tatcgcgaca ggtcgagcat tttatgaagc
     3001 acaagcacca gttgctgaca cagatttaac agtaccatat atttgtttga atggtgctga
     3061 agtacgtgat gaaactttca atgtaatgag cacttcacac cttaataaat cgttagtaca
     3121 caaaattaca aatgttttaa aagatgcagg tatttattat caagtataca cgagtcgtgc
     3181 gatttatact gaagatccac aaagagattt agacatttac atagatattg ctgagcgtgc
     3241 aggtcaacat gcaaacgttg agcgtattaa aaatggtatt caaagacgca tagataatgg
     3301 tacgttgaaa gttgttgata attatgatgc tattgaaaac atacctggtg aattaattat
     3361 gaaaatatta gcatttgatg gaaatttaga aaaaattgac aaagctagta aaattttagc
     3421 tgaatctccg aatttagcta tatcatcatc ttcgagagga aatatagaaa taacgcattc
     3481 agatgcacaa aaaggtattg cgctagaaac aattgccgaa agattaggga ttgaaatgaa
     3541 agaagtcatg tcaataggtg acaatttaaa tgacttatca atgttagaga aagttggcta
     3601 tccagttgcg atggaaaatg gtgcagaaga agttaaaaaa atagcgaaat atgtcacaga
     3661 tacgaatgaa aatagtggtg ttggaaaagc tattatgaaa ttattacgtg aacaacaagt
     3721 ttaataaaaa aagaggggtc aaatatgaaa ggattaatta ttattggcag tgcacaagtg
     3781 aattcacata caagtgcact agcaagatac ttaactgagc attttaaaac acatgatatt
     3841 gaagcggaaa tattcgattt agcagaaaaa ccgttaaatc aattagattt ttcaggaaca
     3901 acaccgtcta ttgatgaaat caaacaaaat atgaaagatt taaaagagaa agcaatggcg
     3961 gcggactttt taatattagg aacgccaaac tatcatggtt catattctgg aatattgaaa
     4021 aatgcattag atcatctaaa tatggattat tttaaaatga aacctgtagg cttaatagga
     4081 aatagtggtg gtattgttag ttcagagcca ttgtcacatt taagagtaat cgtcagaagt
     4141 ttactaggca ttgctgtacc aactcaaata gcaacacatg attctgattt tgctaaaaat
     4201 gaagatggtt catattactt aaatgatagt gaattccaat tacgagcaag attatttgtc
     4261 gatcaaattg tatcttttgt gaataatagt ccatatgaac atttaaaata atattaaaaa
     4321 atatgtaaat atacattaaa aataggtatg tagcttaagg tatttcattg agaatgcttt
     4381 taagtcacat gcctattttt tgttctaatg caccaatagt aagcctttga tattactgtt
     4441 gtgagcaatg ttattaatta aaataagatg taatagcgaa ttgaaaacaa gcttagatta
     4501 aaagtgagtt ttaaaaatat agataaataa tttaaagcaa ttaaagaaaa aaagtattaa
     4561 aaattgcaat tcttaaactg tgattcatat taatatttct agcaaataac attgtaaaat
     4621 aaagaaaaat aattaaagta ttgcacttta ttgaaattta tattacgata gtaatgcaga
     4681 aatttatata tgcaaaatat tatatttatc aaattttgat attttaaagg agtattatta
     4741 aatgaataat aaaaagacag caacaaatag aaaaggcatg ataccaaatc gattaaacaa
     4801 attttcgata agaaagtatt ctgtaggtac tgcttcaatt ttagtaggga caacattgat
     4861 ttttgggtta agtggtcatg aagctaaagc ggcagaacat acgaatggag aattaaatca
     4921 atcaaaaaat gaaacgacag ccccaagtga gaataaaaca actaaaaaag ttgatagtcg
     4981 tcaactaaaa gacaatacgc aaactgcaac tgcagatcag cctaaagtga caatgagtga
     5041 tagtgcaaca gttaaagaaa ctagtagtaa catgcaatca ccacaaaacg ctacagctaa
     5101 tcaatctact acaaaaacta gcaatgtaac aacaaatgat aaatcatcaa ctacatatag
     5161 taatgaaact gataaaagta atttaacaca agcaaaagat gtttcaacta cacctaaaac
     5221 aacgactatt aaaccaagaa ctttaaatcg catggcagtg aatactgttg cagctccaca
     5281 acaaggaaca aatgttaatg ataaagtaca tttttcaaat attgacattg cgattgataa
     5341 aggacatgtt aatcagacta ctggtaaaac tgaattttgg gcaacttcaa gtgatgtttt
     5401 aaaattaaaa gcaaattaca caatcgatga ttctgttaaa gagggcgata tatttacttt
     5461 taaatatggt caatatttcc gtccaggatc agtaagatta ccttcacaaa ctcaaaattt
     5521 atataatgcc caaggtaata ttattgcaaa aggtatttat gatagtacaa caaacacaac
     5581 aacatatact tttacgaact atgtagatca atatacaaat gttagaggta gctttgaaca
     5641 agttgcattt gcgaaacgta aaaatgcaac aactgataaa acagcttata aaatggaagt
     5701 aactttaggt aatgatacat atagcgaaga aatcattgtc gattatggta ataaaaaagc
     5761 acaaccgctt atttcaagta caaactata
//
LOCUS       DANRDI010000156         5751 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.157, whole genome shotgun sequence.
ACCESSION   DANRDI010000156 DANRDI010000000
VERSION     DANRDI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.157"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1376)
                     /gene="bshC"
                     /locus_tag="PZ377_002346"
     CDS             complement(<1..1376)
                     /gene="bshC"
                     /locus_tag="PZ377_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDM6359205.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNE"
     gene            1571..2011
                     /locus_tag="PZ377_002347"
     CDS             1571..2011
                     /locus_tag="PZ377_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDM6359206.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            complement(2120..2815)
                     /locus_tag="PZ377_002348"
     CDS             complement(2120..2815)
                     /locus_tag="PZ377_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDM6359207.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(2939..3073)
                     /locus_tag="PZ377_002349"
     CDS             complement(2939..3073)
                     /locus_tag="PZ377_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDM6359208.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            complement(3130..3264)
                     /locus_tag="PZ377_002350"
     CDS             complement(3130..3264)
                     /locus_tag="PZ377_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDM6359209.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            3628..3701
                     /locus_tag="PZ377_002351"
     tRNA            3628..3701
                     /locus_tag="PZ377_002351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3662..3664,aa:Arg,seq:tct)
     gene            complement(4176..4364)
                     /locus_tag="PZ377_002352"
     CDS             complement(4176..4364)
                     /locus_tag="PZ377_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDM6359210.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            4613..5560
                     /locus_tag="PZ377_002353"
     CDS             4613..5560
                     /locus_tag="PZ377_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDM6359211.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
BASE COUNT         1830 a         1005 c          777 g         2139 t
ORIGIN      
        1 atttcattat ttttattcac aaggttaata ttattttgat tccccgccac ttcatctaat
       61 aagtctttgt ttagacgacg ctgttgttca atcatacctt ctaccttttc aataaattga
      121 tgtgatgctt gttctctaat aaacttactt ctttctcctt caacaccatc gactaacact
      181 ttttctaatg gaatattgta tttcgaaagt aatttttcta tacggtcatt taaataagta
      241 attctaagcc ttggcatcac gataggcatt tcaacatcaa atagttcaaa tacatctttt
      301 agttcagccc agtacttaat ttcactcggt cctccaacaa atgccaccgt gttaaataac
      361 cattcttcca ttaatggtct tgtcactaca ttattagaaa ataattcagg ttgattttcc
      421 gcaatattta taatttcttc ctttatatat gtcttatctg ttttatttaa tttaaaatgc
      481 ttaccatcat acgaaactaa ttgacgcata ttttcatcat gtaagaataa atgaacattt
      541 gtatctgttt gtatcatcgc attcaagcct tgattttgag tacgttgttg tgttgctcta
      601 aaagcatcat caagcaactg atgttttttc aaaatctttt taaacatagg cgcttccatt
      661 tttcttaacg gctcaaactg cgcatctata aatagaacgc catatgcttt aaatgtttca
      721 tgcagtagtg ctttaaacat atcagtccac gagtcatatt ggtcaataat tctgtcacaa
      781 atctccagta gaccttgtgt atgaacagtt tctttcatat gaatgaacat cgtttttaaa
      841 gtttgtttca actcagcctt atcaggataa tatctagaga cagtcgtctc tggcatctcc
      901 attgtatgat atttaacctt atgcagcgac ccatgatttt cgttataaac aaatgtatga
      961 ttcacttcat cgaaatcatg atcttctcct gcaatccaaa aaactggtac tacttgttgc
     1021 ttatgtgtat ccgttaattc cttagataaa gtaatgattg aaaatatttt atggaatgta
     1081 tacaatggtc ccccgaaaag ccctgcttgt tgtccaccaa tcacaacttt tgaaccatta
     1141 gctaaatgtt gtatgtttaa ttcttgttca cttgaaagct ttaaatcact catatattca
     1201 cgaataacat tcgctaatgc cgcttctctt ccattatttt ctttagacat ccttttttca
     1261 aaactagttt gttgagctgc atcatattga aataatcctg ttattacagg gtcactgctc
     1321 tttatttttg gtataaactg atctttttca tttaaactaa ctactttaca gtccatgttt
     1381 tttctcctta agtacgcgat tacaaactat agtataaagt ctataccggt gattgacaat
     1441 tttacggctt gaaaatcaat ttaatcatgg aaaatttata atattcattg ttttacattt
     1501 tcaaatcaat gaaaaacaca agtggtttaa tgtataataa tagtagtaaa caaataaggg
     1561 gtagataaat atgagtgaaa tcaaacgtct tgaaattaat tacaaaactg acgaattatt
     1621 cgaaaacttt agagcgtttg gcaacaaaga cttatacatg gtcaatgagt taaacggtca
     1681 aatgattgat gcaagttcag attcaccatt ttatggcata tttgtcggag atcaattagg
     1741 agctagaatg gcattactaa aaaaaggtga tgtcgaagaa atctacttcc cagattttga
     1801 agattatata ttattatgga agttagaagt attaccaaaa tatcaaaaca gagggtacgc
     1861 ttcagaattg attgattttg caaagagttt caatatgcca attaaagcca ttggcagaaa
     1921 tgattctaag gatttctttt tacatcatgg atttacagat gtggaagcta aaaatataga
     1981 gggacatgat gtcttattgt ggaaaccata agataataat attcgacact acgagcatga
     2041 aaatgcatct tttcgtagtg tcttttttac aattactttc ttaagctaat ataagtaaat
     2101 cattttcaaa ttatttgtct taacgtacaa tatcatttag ttgtttccat gaattaattt
     2161 cataatcagg tataattcct ggattatgat caaatcctct aaaattaaac cagcaagtag
     2221 ctatacccgc attgattcca cctagaatgt cagatgttaa agaatctcca actataatcg
     2281 agtgctgtct ttcatcctca ccaatatcat taaaaacata attaaaaaat tccggattag
     2341 gtttttgata tcctgtttct tcagatataa atatcttttt aatatattta tgcaacggcg
     2401 tctgatttaa ccttctcttt tgcgtttcgg ttacaccatt agtaacaata tataaatcat
     2461 gtcttttcga taattcgaca attgtttcta atgtttgatc aaagtattta actttagctt
     2521 ctgctaatcc atttctaaat aacacatctg cacgatgccc atcaacttcc atttgatgat
     2581 gtttgaagta attcacaaat cgttctgata atacttcaga cttcgttaat ttattttgtt
     2641 gaaaagcttc ccaatgttgg tgattgattt ttttaaatgt taaaaaatca tcctttgttg
     2701 ctttatgatt aaaaacattc gccatatagt gaaacgccca ttcttctgca tcataaaaat
     2761 caacaattgt atcatcaaag tctatcaaaa tatttttata tcccaatttc cccatctcct
     2821 atattgtcta tgtatctaaa tcttaacaga ggctcaaatt tctgcaaata aaataaactg
     2881 agtgcataac attaaagtat gctcacccag tttattttaa agaatattag ttattatatt
     2941 agaatccaaa taatttacct agtaaaccca caccgttagc aacgatgtct acgatacttg
     3001 tgcctaattt cacactatca tgttgttgtg cagcttgcac agtatttgcg attgcttctg
     3061 ctagtccagt catttaaatc tctccctcac ctttgaaata atactgatta cttacataac
     3121 atattgaaat tagaatccga ataatttacc taataaacct acgccatttt caacgatgct
     3181 cacaatgctt gtgcctaatt ttgcgccatc attattaatt gctgcagtta cggtatcttt
     3241 aattgcgtta aataaacctt ccattgaaaa cactccttaa aatttaaatt tgaagataac
     3301 aaaaacgtgc gtagctttta aatcaccgaa atgttattcg cttatcgttt tgttgttgtt
     3361 attttaaaat aaatttgata caattagttt gtttatccgc acaacatctt ataatgtact
     3421 taactgtatt ttaaagagaa aagaaataca gttaggcatt caaaactgta tttaacacaa
     3481 ttaagttgcc tgaattcgta tttaagtctt attgaacctt tttagataaa tagctctata
     3541 atagtgaaaa atataaacat tttttattta caaggtattg ctaatttaag ttcatttaga
     3601 tataataatt cttgtgttgt taaacgtgtc ctggtagctc agctggatag agcaatggcc
     3661 ttctaagcca tcggtcgggg gttcgaatcc ctcccaggac gtttataggt atttttatac
     3721 gcattaccaa acaaaagagt tccgtgatta cggggctctt tttgttttga atttcagtaa
     3781 tatagtatga tgcgtcacca aaacgtcccc cgcataagcc ccgaaaatac agtaattaaa
     3841 acaagcatgc ttattcgtta tagaattttt tgacacacaa ttgacacgcg tctgacactt
     3901 gtttatacat ttttaattaa gtaattttgt gctcaaattt catctatact gcacctgaac
     3961 tacaccaaca ctacaccaag atttttaaca ctcaccattt gcatgcgtag agatttttat
     4021 tattatatta ttcctataga ttttgatact attcaaaatt ttagggactt ttcaggggcc
     4081 cgaaatccta taattataat tatatacatc taaaaaaaat aaccacgtcc atcgagacgt
     4141 ggttttttaa ttatttaact cactaattat tcttcttaat tttcattgct aaatatacta
     4201 ttggcaaatt tataaagtag ttcagcgttt ttcaatgaca tattgtctaa tgatctttca
     4261 ttttttctca ttctgtgtat tgtgctttgt ggaactcctg tttgtttcga tatatgtaaa
     4321 ctgctcaaat cactgtctaa tagtttttga atttgatttc tcattttgta cacccccttg
     4381 taaatcttca atcatcattt aaattaaata attacttttc cacatattcc aatatttagg
     4441 ttgcaaagca tacctcaaat atcattaatt ttgagattta aatgtcaatt atgtgtctat
     4501 caatccaata tacatactct aataacgtaa tagtacacac tcttcttatt aattggccat
     4561 agctatcatg atataattag tgaagaaaat cacataagaa aggttgtaaa tcatgagact
     4621 tcaaaaagca cctctagtaa cgtcaggact agtcttagga ttattaggcc tgggtaatct
     4681 attaaaagac ttatctctta ctttaaacgc tgtttgcgga atctttgctt tcttgatttg
     4741 gattcacctt ttatgtacta tgatcaaata ttttaataat gtgaaagaac aattaaacag
     4801 tcctctagtt tcatcagtgt tcacaacatt tttcatgtct ggctttttag gtactactta
     4861 tttaaataca ttttttagta acataacttt tatcaatagc ttaataacgc ctatttggat
     4921 tttatgcctt gtgggaatta tgacgcatat gattattttt tcaataaaat atttaaaaga
     4981 tttttcactt aaaaatgttt atccttcgtg gactgtactt tttattggta ttgctatcgc
     5041 aggattgacg gcacccgtta gcggatattt tttcataggt caattaacag taatatatgg
     5101 ctttgtagct acttgtattg tcttacctat agttttcaag cgattaaaag catttccatt
     5161 gcagacgtca atcaaaccga acacatcgac aatttgtgca ccattttctt tagtcgctgc
     5221 agcatatgtt atagcttttc ctaaggcgaa tgcttttatc gtaattatat ttttactatt
     5281 agctcaaata ttttattttt atatcattat acaattgcct aaattactaa aagaaccttt
     5341 ttcgcccgta ttttcagctt tcacattccc tttagtaatc tcagcaactg ctttaaagaa
     5401 cagtttgcct gtacttatgt ttccagacat ttggaaaggt cttttgttta tcgaagtgtt
     5461 attagccact gtaatagtac ttagagtctt tataggatat cttcacttct ttttaaaaaa
     5521 ggaaaaacaa gataaatttc ttcgtaatgc gtctcagtaa cactattacc aagaattaac
     5581 acgtatattt aataactaaa aagaggcagg taatcttgcc tctttttagt gtatatcaat
     5641 gcctctttga tttaatatga tcttatcaat gataccttct gactaaagct atagtaacta
     5701 tactagaacg tctagttcat aaacacgttt cactctaaac tttatttaaa t
//
LOCUS       DANRDI010000157         5740 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.158, whole genome shotgun sequence.
ACCESSION   DANRDI010000157 DANRDI010000000
VERSION     DANRDI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.158"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            1..656
                     /locus_tag="PZ377_002354"
                     /pseudo
     CDS             1..656
                     /locus_tag="PZ377_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            695..1633
                     /locus_tag="PZ377_002355"
     CDS             695..1633
                     /locus_tag="PZ377_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDM6359212.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(1899..2141)
                     /gene="rpsR"
                     /locus_tag="PZ377_002356"
     CDS             complement(1899..2141)
                     /gene="rpsR"
                     /locus_tag="PZ377_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDM6359213.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(2193..2696)
                     /gene="ssb"
                     /locus_tag="PZ377_002357"
     CDS             complement(2193..2696)
                     /gene="ssb"
                     /locus_tag="PZ377_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDM6359214.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(2717..3013)
                     /locus_tag="PZ377_002358"
     CDS             complement(2717..3013)
                     /locus_tag="PZ377_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDM6359215.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            3257..3448
                     /locus_tag="PZ377_002359"
     CDS             3257..3448
                     /locus_tag="PZ377_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359216.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(3534..4631)
                     /gene="ychF"
                     /locus_tag="PZ377_002360"
     CDS             complement(3534..4631)
                     /gene="ychF"
                     /locus_tag="PZ377_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDM6359217.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(4643..4846)
                     /locus_tag="PZ377_002361"
     CDS             complement(4643..4846)
                     /locus_tag="PZ377_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDM6359218.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(4876..>5740)
                     /locus_tag="PZ377_002362"
     CDS             complement(4876..>5740)
                     /locus_tag="PZ377_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDM6359219.1"
                     /translation="IISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKILNKMIE
                     QGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVIASAGVV
                     GLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGLRSTRIN
                     TISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTSLRSKYY
                     LFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGIKTPQPI
                     MTAYNHSENGV"
BASE COUNT         1917 a         1039 c          832 g         1952 t
ORIGIN      
        1 agctaaacat aataacacat tagaagataa tgaagttaag gagttactgg attgtttcga
       61 ctatgtaatt aagtataaaa atatccaacg acaaaacgta attataaaat ggtaaaagct
      121 atggtacagt ttcaaattgc taatgacatg cgtatcggtg agctacttgc aataaagaga
      181 gtaaatataa actatgaaga taaaacgcta gatatcgacg gtaaagttaa ttggataact
      241 gaaaaaagac gggagcattc ggagtaaagg agacaactga aagaagtaat agctataagg
      301 ccacagggct cactacccaa agcatcgact tactaagaac acttatgctt ggaatgataa
      361 gtttattgat agagagtaca tattcacaaa tacgtctggt agccctatcg actcgaacaa
      421 aattagccac attattaaag gggggcgctg atattagttc tattaagaaa cctataacga
      481 cgcatacatt acatcattcg catatatcta cacttgctca attaggaatt aacttaaaag
      541 caatgcaaga gcatgtaggt cattcagatt ataaaaaaaa tctagagata tacacacatg
      601 ttactaatca gatggcgaaa gatatgatga ataaatttga acgattgggg agttaaaatt
      661 ggaaaaagat gatacactag cagaaattaa gcctatgctc aattttgatg agcaaatagc
      721 gaaattaaaa cagatgaata tattttttaa tattattgac accgaaaaag caaatgaaat
      781 tcttagaaaa aataattact tcttcaaact agcttatttc cgaaaaaatt tcgaaaaaaa
      841 gaatggcggc tatttcatag aatttgctta tttatcagat ttagcaacta tagatatgaa
      901 attaagatac acaatgttgc atttaacttt agatattgaa catagtttaa agtatctagt
      961 cttaaaacta ataacagaaa ataaccaaga agatggttat aaaataatag atgagttctt
     1021 atgtattgat aaatcatata gcaattcaaa ttttgacaca aattcaagaa caccagaaga
     1081 agttatggaa accaaaatca aaaataaaaa cgaaatattc aagcatatga ataaacgagg
     1141 acaactaccc gagaagttga ataaatacta tcaaaatcca cccgcatggg tttgcattga
     1201 attcatgcaa ctaggtcaat tcgtttcgtt tctcaacttc tattacaaga agtacaatga
     1261 cgaagaattg agagttgcta atattttaat gcctttagtt aaaaatataa gaaacaaatc
     1321 agctcataac caacccatca tagcaaatct aaattatgac agtagattac ctcaatattt
     1381 atttgaaaaa gggaataata taggcatatc tagaaacatg ttcggaataa aaaatttcat
     1441 agatactttc gctacgctag aattacataa tcaagtttgt agtaatgcaa ttatccaagc
     1501 aagatatcac gatttggacc aacttcaaaa gcgatataaa agaaacgaaa gctattataa
     1561 taatgcatta gctatcaaaa gattttttat agctttagat aaaattattg acttcaacag
     1621 accaaaagta taaactatct agtgaggaaa gagacttata ggtctcgcga gttattttaa
     1681 ttcgtatgca agaaaaagaa gagctatgca ttttatttaa aatgcgtagt tcttttttta
     1741 tgcatctaaa ttcatattat ttttgcaata taaacatatc tttgtgcaaa ttccgaacac
     1801 aaaacattca catcatcctt ttttgccctt tttctatacc ccaaaacaca aaaagccccg
     1861 taagcctatg cctacggggt ttgacaataa attatatatt attgttcttc tttaacatat
     1921 ggtaataatg ccatatgacg agaacgtttg atagctgtag tcaacatacg ttgatattta
     1981 gctgaagtac cagttacacg acgtggtaaa attttaccgc gttctgagat aaaacgtttt
     2041 aataattcag tgtctttgta gtcgatatgt gtaataccat ttgctgtgaa atagcatact
     2101 tttttacgac gacgtccgcc tcttcttggt ccacctgcca tgattgtgtg cctcctttga
     2161 taattttttc gctttaattt cgttaatttt tattagaatg gtaagtcatc atcacttata
     2221 tcaatcggtc cgtttgcatt tgcaaatgga ttatcagatt gtttcgtgtt tgatgaatta
     2281 ttgtacgaat tgttttgtcc tgattgttga ccaccgaatc cttgaccgta atcttggaat
     2341 tcattttgtt gacgttggcc accattttgt tgcgcatttt taggttcaag gaattgaacg
     2401 ctatcacaca caacttcagt aacaaacaca cgacgacctt cttgattttc ataattacgg
     2461 gattgtaagc gaccatctac accagctaaa ctacctttag ataaatagtt atttacatta
     2521 tctgcttgtc ttctaaaaac aacacagtta ataaaatctg cttcgcgctc cccttgagca
     2581 ttcgtgaacg tacgatttac tgcaagagtg aatgtcgcta cactcacacc tgagggagtg
     2641 gttctgtatt ccggatcttt cgttaaacga cctactaata caactctatt tagcatttaa
     2701 acgccccctc taattattac ttgtcttcgt cttcacgaat aaccatgtaa cgaatgatat
     2761 cgtcactgat tttagctaga cgttggaatt cgtcagtagc tttgttgtta tcagatttaa
     2821 cacgtacgat gttgtagaag ccatctttga aatcattgat ttcataagct aggcgacgtt
     2881 taccccagtc ttttgcttct aaaacttctg caccttcagt agctaagata ccgttgaaac
     2941 gttcaactaa cgctttttta gcatcttcct caatgtttgg gcgtacgatg tacataactt
     3001 catatgttct cattttatat ttgcacctcc ttgtggtcta tacggcttat caatcttaaa
     3061 acagataagc aaggaataat tttcattact cacaataaag aattatatca tgcgccatta
     3121 ctttttacaa taataattca aactactctt catatcattt ttgatattaa ttcatttgaa
     3181 actttctatg atatttttaa aaatacactt cacaaaagcg aacatatgtt ctataatagt
     3241 tgtgaggtgg taaggaatga atttaaatat tgattggtct aaagattttc aagaattcca
     3301 agagatactt aatagtggta ttcatcctga atggctttat tgtgcaaagg ctaatcttgt
     3361 tttagagcct gcttatactg gcgaaggcaa acaatttttc agcactcaag atattattaa
     3421 cgcgagtaaa attattccat tcttttaata aattatacgc ataattaaaa gaggcgaaca
     3481 attgattccg cctcttctac atatatacta tttaattaac ttcactctat cgtttataca
     3541 ttaaatctga aatgaacgat atcgccatct tgcataatat attctttacc ttctaatcgt
     3601 tgtctgcccg cttctttagc gccactttcg ccaccatatt gtacatagtc gtcataactt
     3661 gttacttcgg cacggataaa tccacgttca aaatcagtat gaatgatacc agcacattga
     3721 ggtgcagtca taccttgttt aaatgtccaa gcacgtactt cttgcacacc agcagtaaaa
     3781 tatgttgata atcctaataa ttcataagtt gttctaatta atcgatctaa tcctggttct
     3841 tcgataccta aatcttctaa gaacatttct ttatcttcat catctaatgt agcaatttct
     3901 tcttcaattt ttgcactaat aacaatcact tcagagtctt cttgcgctgc atattcacga
     3961 atcgctttta ctttatcatt atcatcatca ccaatttcat cttcaccaac attagcgata
     4021 taaagcattt ttttagaagt cagtaattgc gcttgattca cccatttttg atcttcttca
     4081 ttaaagtcaa tactacgagc gggtttacca ttttctaaag cttctttaat agttgttaaa
     4141 atacgtactt ccatttcagc agtcttatct ttttgacgtg ctaatttttc aattctaggc
     4201 aaacgtttct caacagattc taagtccgct agtactaatt ccatattaat aacttcaata
     4261 tcatcaatag ggtctactcg accagcaaca tgagttacgt tatcatcatc aaatgcacga
     4321 acgacctgac aaatcgcatc tacttctcta atatgtgata agaatttatt acctaaccct
     4381 tctccctttg aagcaccttt cacaatacca gcgatatctg taaattcaaa tgtagtcggc
     4441 aatgtctttt taggttgaac catttcttct aatttaagta atctagcatc tggcacttct
     4501 actatcccta cattaggatc aatcgtagcg aatggatagt tcgctgctaa agcacctgct
     4561 tttgttattg cattaaataa tgttgattta ccaacgtttg gcaatccaac gatacctgct
     4621 gttaaagcca ttaatcattc tcctatcttt gtgtatcatc atgagattcg atgatttttt
     4681 taagtttttt atcaaacgtt tgacgtggaa tcataatact tctttgacaa ttttcacatt
     4741 taattcttat gtctgcaccc attctaataa tcttaaaacg gtttgttcca cacgcatgtt
     4801 gttttttcat ttctactata tcatttattc catattttga cgccattaaa tatgacctcc
     4861 atgtattata aactactaaa caccgttttc actatgatta taagcagtca taattggttg
     4921 aggtgtttta ataccttctt gtaaaaacat tttttgtact tctttgcgaa taattcgagc
     4981 tccagcaaat ccttcacctg gaattgtttc tgcagatatt ctcaatatta ctcttgtatc
     5041 ttcaatagca tcaataccaa taacaaccgg atcactaaca aataagtaat atttactacg
     5101 taaagaagta aatagtttgt ttagtttttt atcaacatta tcaatgtttt cttcgacaga
     5161 cactggaatt ttaacgatag ctgtaccatt tgtaattgag tagttcgtta tttcacccat
     5221 actactattt ggtaaaattg ttaattctcc tgaaattgta ttgattcgtg ttgaacgaag
     5281 tccaattgat ttaactgttc cctctgctac agttgtacca ccgttattta tcttaacata
     5341 atcacctaca tcaaattggc tttcaaatat aataaagaat cctgtaatta cgtctttaac
     5401 tatagtttga gcaccaaaac ctactgctaa gcctacgaca ccagcactgg caattacccc
     5461 ttcaacacta atgccaaatt tacttaaaat cgtcgtaata actataaacc aaacgatata
     5521 cttcactaca ttttgaacaa gagatattaa agttttagag cgctttttgt tactcttttt
     5581 acttttattt tgaatcttaa atccctgttc aatcatttta ttcagtattt taataactat
     5641 gagggctacg ataatataaa taacaatcat agcgatttta gttgcaatgt tttcatatgt
     5701 ttctattttt gttaatggct caaatagaga tgaaataata
//
LOCUS       DANRDI010000158         5663 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.159, whole genome shotgun sequence.
ACCESSION   DANRDI010000158 DANRDI010000000
VERSION     DANRDI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.159"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(654..1847)
                     /gene="sqr"
                     /locus_tag="PZ377_002363"
                     /pseudo
     CDS             complement(654..1847)
                     /gene="sqr"
                     /locus_tag="PZ377_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(1866..3200)
                     /gene="cstB"
                     /locus_tag="PZ377_002364"
     CDS             complement(1866..3200)
                     /gene="cstB"
                     /locus_tag="PZ377_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HDM6359220.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(3231..4298)
                     /gene="cstA"
                     /locus_tag="PZ377_002365"
     CDS             complement(3231..4298)
                     /gene="cstA"
                     /locus_tag="PZ377_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HDM6359221.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            4435..4695
                     /gene="cstR"
                     /locus_tag="PZ377_002366"
     CDS             4435..4695
                     /gene="cstR"
                     /locus_tag="PZ377_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDM6359222.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            4695..5450
                     /locus_tag="PZ377_002367"
     CDS             4695..5450
                     /locus_tag="PZ377_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDM6359223.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            5548..5649
                     /locus_tag="PZ377_002368"
     CDS             5548..5649
                     /locus_tag="PZ377_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359224.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
BASE COUNT         1792 a          986 c          797 g         2088 t
ORIGIN      
        1 gtttatggat gatatttccc aatttaacac ggattgaaca ctttctactt aggtattatc
       61 ttggttttcc tgataggata actcccgatg atgccttttt acttatgtca cttatccttt
      121 tggaaatggt gcaaaattaa aaatacatca ggatatcgtt taactcagaa aaaaactcca
      181 atcaaaaaaa atgtttacaa cacattatga aattaaaatt gctcttagtg ttcaacagac
      241 aaagttattg aaattactat tggaggtaca taccaagcta tcattttgag tacacgtggt
      301 ttgaatatct ggcaagaaat ggaaattggt aatgtcagtc ataaagtcgc taaacgtgct
      361 caaataccat taattatttt taaataattt agaggatgag gtataaacct ttaaaaaaca
      421 gcagtgagat gattttcaat tagaaaatat cttactgctg tttctatttt cataatcatt
      481 cttattgaat gacttcatcg tgatcattta aaacagtaac aactttttca atcttatact
      541 atttagagct aaaaattcac gacatataaa cacaacactg acacgtcatt taaatactta
      601 atcttttata gtaaaagatt tttcattaaa aatttattgt tttctgtact ttattatatt
      661 aatcctttta gcatgccgta ccaatacatt ttaggtaata aatctttctt aaatatatac
      721 atacttctac gttctttggc ctgattaaac ggcattgttt ctttagtatt tttattataa
      781 tcaaactctg caagtattaa cctattatat ccagtaacaa tagggcatga agtataacca
      841 tcataatgat gcgttaacat ttgattattc atcacttgca ataaattatt agcgacgata
      901 ggtgcttgct tacgaatagc tgcgcctgtt tttgaagtag gtacatttga agcatcacca
      961 agtgcaaata cattagagta gcttttatgc tgtaatgtgg ttgggttaac atctacccaa
     1021 ccctcactat ctgaaagtgt actttctttt actacatcta agggacccat aggtggtgtt
     1081 acatgtaaca tatcataact tattgttttt ctatcgtatg ctttgatatg ttcgaatgta
     1141 gccacttttt tgtcaccgtc gatttcaaca aggttataat tataattgac tgttatattt
     1201 ctttcttcaa caatcctttc taattcttta ttatattttc ctacgtcaaa taaagcatct
     1261 tttggcgttg catatatcac attagcgtta gaacggattt tatgtttcct aaaataatct
     1321 tcagctaaat acataatttt cataggcgca cctccacact ttataggagt gtttggatgc
     1381 gtaaaaatgg catttccttg tttaaaatta gaaatttggt tccaagtttc gttaacatag
     1441 tcaggtgaat agttagagca aacaccattt ttacctatat tttcttttag tcctttaatt
     1501 gaagaccaat taatctgtaa tcctggagct actactaaaa aatcataata aacgactgta
     1561 ttatctccta aaataacgct attattttca ggttaaaaac ttgaaacagc ctgttttatc
     1621 cagttagcac cttcaggtat aacactttcc atatctttac gagaactttt caaactagat
     1681 acccctgcac caaccaacgt ccataatggt tgatagtaat gatggtctgc tggatctata
     1741 attgctattt tctcttttaa gttttgattt tttcttaata gtcttgatgc tacggtaaca
     1801 cctgctgtac cgccaccaat aataacaatt tgataatgct tattcatttt ttccacatcc
     1861 ttaaattatt ttaatgattc tggaaaatct tgatagcctt ctctaatatt caccacattt
     1921 tcaaaacctt tgctttccaa tatacccact gcaattgaac ttctaacacc tgactgacaa
     1981 tgtacatata ttttatcctc tttattaaaa ggaatatttt catttaataa tttaccatgc
     2041 ggaatattaa ctgcttgatc taagtgtcca ttattccact cttcatcatt acgtacgtct
     2101 aatacatgtt cttctttacc tgtcatatca gcgctatgaa cggactgggt tgaaatgcct
     2161 gattttggca aacgatagcc tgctacctta tcaaacccaa ttaattgtaa agtgtgtttc
     2221 gctttctcaa cagtagattt atctccaatt aaatctatat ctttttcaaa atctaagtac
     2281 caaccaattt gattaataaa gtttttgttg tatggtatat tgattgttcc ttttgtgtgg
     2341 ccaccgtgaa aggcctcttt gctacgaaga tcaaatgcta ctctcttatt atctaaacta
     2401 ggataaacat catatgattg atataaattc ataccaaact gattaacttg tttcatttgt
     2461 gcaaaatgat gcggtggtgc tggttgattt gatgttaatg attcaataaa tttagtctca
     2521 tcaatttcat taaatgccca gttattaatt ttctcataac ctattgtaga tataggtatg
     2581 gcacctaatg ctttaccaca agggcttcca gcaccatgac ccggccagat ttgaacatag
     2641 tctggtaaat ttttaatatt ttgaacggac tcatacattt gtttcgcgct aatttctgta
     2701 gaacccttta tttgaacaga tttttctaat aaatcaggtc tacctatatc accaacaaaa
     2761 ataaagtcac cactaaataa tcccatcgga acacttgaac caccacctaa atcagtgagt
     2821 aaaaagctaa tactttcagg cgtgtgtcct ggagtatgca gaacttctaa tttaacattg
     2881 cctacttgaa tgatatctcc atgtttaaca aattgtgttt ttgatggcat atttttatac
     2941 cctaatgcat cttcaccttc gccagacaca tatatatttg catttaagcg tttagccaca
     3001 tcacgaattc ctgaagcaaa atcagcatga atatgtgttt ctgtagcttg tgtaattgtt
     3061 aaaccttcag aatctgcaac ttctatatat ttcgataaat cacgaacagg gtctattatt
     3121 attgcctctc ctgtacgttg acaacccact aaatatgatg cttgagataa atgattatcg
     3181 taaaactgtt taaaaaacat gataacaact ccttttatta atagatttaa ttagataaat
     3241 aaattatgat tcgcattttc agtagcacca atataagtgc ctacaccacc gtactcaact
     3301 tcatctctaa gttcttcttt ctgaataccc atgacatcca tactcatcgt acaagcgatt
     3361 aatttgatat tttgctcaat agcttgattg ataagtgttg gtaatgaatc aacatttttc
     3421 tttttcatta cgtagcgcat catcatattt cctaaaccaa acatattcat tttggaaaga
     3481 ggcattcgta tattcttttt gggcaacatt aaatcaaaca tttttgcaat accttgcttt
     3541 ttaacattaa ctgtttgcac tttttttaat gcattaagcc cccaaaaagt aaagaagata
     3601 gttacatctt ttccagcagc tctagcacca tttgcaataa tcaacgctgc tacagctttg
     3661 tctaattctc cactaaataa tacaattgta gtacctttag cagaataatt tatatctaaa
     3721 tcttttgctt tttctttttg aataatcgca ttaattccat tgttattttc atcaagctta
     3781 actaaagtat gccttgtttg tttcacccaa cttttaatgt cactagggaa tccagggtct
     3841 gtgactttga cttcaatttg gtcacctact tcaatattct tcatttcttg actaattttt
     3901 acaattggcc ctggacattg aagaccacga tagttaaatt gtttacgatc tgctttaatt
     3961 tctatacttt tatattcttc ttgtgtatca taactatcgt tttcttcttc ataagcttta
     4021 taaccaccat caagatttat aacgttataa ccttgtttag ctaaatattt actagcatta
     4081 gcacttctgt taccacttct acagattaca taataggttt tatttttttc tttattgaat
     4141 gactcaatct catcaacagg atgaagtatg gaaccattga tatgtcctaa tgcatactcc
     4201 tcttctgttc taacgtcaat taattgccct tgcttggcta gtttttccaa ttctgcttta
     4261 ctaaattcat cgataaactt ttctccgtat tgtttcataa taacctccct aaaaatacct
     4321 gtaggggtat tttaaacagt tggatggaac ttgtcaaata cctctagggg tatttgacac
     4381 atttgtgttt atctattaat atatttttat tcaattaaaa gtggaggtat gactatgaat
     4441 tatgataaaa aaatgattaa tcgtattaat agaatacaag ggcaactaaa tggaattatt
     4501 aaaatgatgg aggaaggaaa agactgtaaa gatgtcatta cacaaataag tgcatcaaag
     4561 agttcactcc aacgcttgat gggtatcatt attagtgaga atttaataga atgtgtaaaa
     4621 gcagctgcgg atgatgaaga aagctcccaa gagttaatta atgaagctgt aaacttattg
     4681 gtgaaaagta agtaatggat attgttaata tagttatcat gttactcatt ggggtattcg
     4741 gtgggttcat atctggtcta gtaggcgtag gtggtgctat cattatttat ccagctattt
     4801 tattattacc accattattc ggagcccctg catatagcgc atatatcgca tcaggtctta
     4861 cttctagcca agtctttttc agtacattaa gtggctcatt aaaagctcga aaaaaaacag
     4921 agttctcgcc acaattagtt ctttatatgg gtggcggcat gattataggg agtatgttag
     4981 gtgcatttct agcaaattta tttgatgcta catttgtaaa tacggtatat atcattattg
     5041 ctttacttgc attaacatta atgtttatta aagtgaaacc tagttccgaa aagtcttcat
     5101 ttaataaata tttattagtt atcattggcc ttttcattgg tattatttca ggtatagtag
     5161 gtgcaggtgg tgccttcatt attattccta tattattggt tttattcaaa ttaccaatga
     5221 atactgtagt cgctaatagt atagtaattg cttttatatc ttcaattggt gcttttgtca
     5281 tcaaacttat tcaaggttat atccctttat atgacgcctt attccttata atagggagta
     5341 ttatatttgc tccaataggg ttaaaattaa gtaaaaaagt ccctaacata atacaaaaat
     5401 ggattatcag tattttaatc gtttttgcta tagttcaact cattttataa aactaatcct
     5461 tttaactcag acagcaattt cccgaaaaat aatacctctt atttaaacaa atatgtatgc
     5521 agttcggatt tatataaata aaaatttttg atgaaatatc catatatgct tataaatata
     5581 aatttatcaa ctataactaa aaatgataaa agatttaacg ccactgatga ccgaattaat
     5641 attatttaag tttttatcaa acg
//
LOCUS       DANRDI010000159         5620 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.160, whole genome shotgun sequence.
ACCESSION   DANRDI010000159 DANRDI010000000
VERSION     DANRDI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.160"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1434
                     /gene="recN"
                     /locus_tag="PZ377_002369"
     CDS             <1..1434
                     /gene="recN"
                     /locus_tag="PZ377_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDM6359225.1"
                     /translation="MDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELL
                     DIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKQELEDLESADQ
                     ALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDENA
                     ITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVL
                     NEYESRMNLLNNLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQ
                     VIEVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFL
                     ISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKM
                     AEKMRDIAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIA
                     RMISGASVTDLTRENAREMIQHNQRRR"
     gene            1585..3006
                     /gene="lpdA"
                     /locus_tag="PZ377_002370"
     CDS             1585..3006
                     /gene="lpdA"
                     /locus_tag="PZ377_002370"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDM6359226.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            3022..4014
                     /locus_tag="PZ377_002371"
     CDS             3022..4014
                     /locus_tag="PZ377_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDM6359227.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            4014..4997
                     /locus_tag="PZ377_002372"
     CDS             4014..4997
                     /locus_tag="PZ377_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDM6359228.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            5010..>5620
                     /locus_tag="PZ377_002373"
     CDS             5010..>5620
                     /locus_tag="PZ377_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HDM6359229.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVR"
BASE COUNT         2063 a          816 c         1119 g         1622 t
ORIGIN      
        1 atggatattg atgtagatga agatttttta ttagttaaac gggaaatttt cagctctggt
       61 aaaagtcttt gtaaaattaa taatcaaaca gttactttac aagatttacg aaaagttatg
      121 caagaattat tagatattca tggccaacat gaaacgcagt ctttgttgaa acaaaaatac
      181 catttaacat tattggataa ttacgcagaa tcacgttatc aagatttgtt ggataaatat
      241 caccaaactt ttcaaaatta taaagccaaa aagcaagagt tagaagattt agaatcagca
      301 gaccaagcat tgctacagcg tttagattta atgaaattcc aattagaaga attgtctgaa
      361 gcacatctaa aagaaggcga aatagagcaa cttgaaatag atattaaacg cattcaaaac
      421 tctgaaaaat taagcttagc gcttaataac gcacatatga cattaacgga tgaaaatgct
      481 atcactgatc gtttatatga actaagcaac catttgttaa caataaacga tatagttcca
      541 aacaaatatg acaaattaaa agaagatatt gatcaatttt actacatttt agaagatgca
      601 aaacatgagt tatatgatga aatggctaat acagaattcg acgaacaagt gttaaacgag
      661 tatgaatctc gtatgaattt gctgaataat ttaaaacgta aatatggaaa agatatttca
      721 gaattaatcg catatcaaga aaagcttaat aatgaaatca ataaaattga aaactatgaa
      781 caaagtacat cgcagttacg agaagaaata aatgcattgt ataatcaagt tatagaggtt
      841 ggacaagcgt tatcaaagca gcgtcgtata gtcgctaggg agttaagaga ccacattgta
      901 tcggaaattc aaaacttaca aatgaaagac gcaaatcttg agatttcatt taaaaaatta
      961 gaagaaccga atattgatgg aatcgaattt gtagaatttt taatcagtcc aaataaaggg
     1021 gaaccattaa aaagtttaaa taaaattgcg tcaggtggag aactttctag aattatgtta
     1081 gcgttaaaaa gtatttttgt taaatcgaga ggtcaaactg caattttatt cgatgaggtt
     1141 gactcaggtg tatctggaca agctgcacaa aaaatggcag agaaaatgcg agatatagca
     1201 gaatatatac aagttatttg tatctctcac ttaccacaag ttgcatcgat gagcgaccat
     1261 catttattaa tttcgaaatc atcaaaagat gatcgtacaa caacacaagt tcaagagtta
     1321 attagcgatg ataaagtaga tgaaattgca cgaatgattt caggtgcaag tgttactgat
     1381 ttaactcgag aaaatgcaag agaaatgata caacacaatc aaagacgtag ataatttcca
     1441 aagtgtgttg aatactcatt tatggattta tttatgtgca ttcaaactaa atagacaatc
     1501 atgtaaaata ggtaattaat ggacatgaaa actatgtatt aataaatttt caatggatat
     1561 atatcattag ttaggagtaa gaacatgtca gagaaacaat atgatttagt cgttctcggt
     1621 ggaggtactg caggatatgt tgctgcgata cgtgcgtctc aattaggtaa aaaagtggca
     1681 atagtggaac gacaactatt aggaggtact tgtctccata aaggttgtat tcctactaaa
     1741 tcgctgctta aatctgctga agtatttcaa acagtaaaac aggcagcaat gtttggcgtt
     1801 gatgtcaaag atgctaatgt taattttgaa aatatgttgg cacgaaaaga agacattatt
     1861 aatcaaatgt atcaaggtgt aaagcattta atgcaacaca atcacattga catttataat
     1921 ggcacgggac gtattctagg tacatctata ttttcacctc aaagtggcac aatttctgtc
     1981 gaatatgaag atggtgaatc agacttatta cccaaccaat ttgtattaat tgcaactggt
     2041 tcttcgcctg cagagttgcc attcttatca tttgatcatg ataaaatttt atctagcgat
     2101 gatatattat cattaaagac gttaccatca agtattggta ttattggtgg tggtgttatc
     2161 ggaatggaat ttgcatcatt gatgatagat ttaggtgttg acgtaacagt tatagaagca
     2221 ggtgaaagaa ttttaccaac tgaaagtaaa caagcttcac aactattaaa aaaatcattg
     2281 tcagcacgtg gtgttaaatt ctatgaggga ataaaacttt ctgaaaatga tataaatgtt
     2341 aacgaagatg gtgttacgtt tgaaatttcc tcagacataa ttaaagtaga taaagtgttg
     2401 ctatcgattg gtagaaaacc taacacatca gatattggtt taaacaacac taaaataaaa
     2461 ctttcaacat caggacatat tttaacgaac gaatttcaac aaactgaaga taaacatatt
     2521 tatgcagctg gtgattgtat aggaaaatta caattggcac atgttggatc aaaagaaggt
     2581 gttgtggctg ttgatcatat gtttgagggg aacccaatcc cagtaaacta taacatgatg
     2641 cctaagtgta tttattcaca acctgaaatt gcttctatcg gtttaaacat tgaacaagca
     2701 aaggcagagg gaatgaaagt taaaagtttt aaagtaccat ttaaagcaat tggtaaagca
     2761 gtgattgata gccatgacac aaacgaaggg tatagcgaaa tggtgattga tcaatcaact
     2821 gaagaaattg tgggtattaa tatgattggt ccacatgtaa cagaattgat taatgaggca
     2881 tcactgttac agttcatgaa tggctcggca ttagaattag gactaacaac acacgcacat
     2941 ccttccatct ctgaagtgtt gatggaatta ggattgaaag cagaaagtag agctattcac
     3001 gtataaatag gaggatttaa catgattgat tataaatcat taggccttag cgaagaagac
     3061 ctaaaagtaa tatataaatg gatggattta ggtagaaaaa tagatgaaag actgtggtta
     3121 ttgaaccgtg caggtaaaat tccatttgtt gtaagtggtc aaggacaaga agcaactcag
     3181 attgggatgg catacgcatt agaagaaggt gatattacag cgccttatta tagagatcta
     3241 gctttcgtta cttatatggg tatctcagcg tatgatacgt ttttatcagc gtttggaaag
     3301 aaagacgatg ttaactctgg tggaaaacaa atgccatcac actttagtag tagagctaag
     3361 aatattttgt ctcaaagttc accggtagca actcaaatac cccatgctgt tggtgctgct
     3421 ttagctttaa aaatggatgg taagaagaaa attgcaacag caactgttgg tgaaggtagc
     3481 tcaaaccaag gtgacttcca tgaaggttta aacttcgcgg gtgtacacaa acttcctttt
     3541 gtatgtgtca ttataaataa taaatatgca atttcagtac ctgattcatt acaatacgca
     3601 gctgaaaaat tatcggatag agcacttggt tacggtatac atggtgaaca agtagatggt
     3661 aatgatccat tagcaatgta taaagcgatg aaagaagcaa gagatcgagc tatttctggt
     3721 caaggttcaa ctttgattga agcagtaaca agtcgtatga cagctcattc atcagatgat
     3781 gacgaccaat atcgtacgaa agaagagcgt gaaacgctta aaaaagcaga ctgcaatgaa
     3841 aagttcaaaa aagagttgct ttcagctggc attatcgatg atgcttggtt ggcagaaata
     3901 gaagcggagc ataaagacat catcaataaa gcgacaaagg ctgctgaaga cgcgccatat
     3961 ccaagtgtcg aagaagccta tgcatttgtg tacgaagaag ggagtcttaa ctaatggcta
     4021 aattatctta tttagaggcg atacgccagg cacaagactt agcattacaa caaaataaag
     4081 atgtgtttat tcttggtgaa gatgttggta gaaaaggtgg cgtatttggt actactcaag
     4141 gactgcaaca aaaatatggt gaagacagag ttatcgatac gccattagct gagtcaaata
     4201 ttgttggaac tgccattggt gcagctatgg taggtaaaag accaattgca gaaattcaat
     4261 ttgcagattt tattttacct gcgacaaatc aaattattag tgaagctgcg aaaatgcgtt
     4321 atcgttcaaa taatgattgg caatgcccat taacgattcg tgcaccattt ggtggcggtg
     4381 ttcacggagg actatatcat tcacaaagta ttgaaagtat atttgcttca tcgccaggtt
     4441 taacaattgt tattccttcc acaccgtatg atgctaaagg gcttttatta tcatctatcg
     4501 aatcaaatga tccagtattg tactttgagc ataaaaaagc ttatcgtttc ttaaaggaag
     4561 aagtgccaga agaatattac actgtacctt taggaaaagc agatgtgaaa cgtgaaggtg
     4621 aagatttaac agtattttgt tatggattaa tggtcaatta ttgtttacaa gcagcggata
     4681 ttttagctgc agacggtatc aatgttgaag tcgttgattt acgaactgtt tatccattag
     4741 ataaagaaac aattattgat cgtgctaaac atacaggtaa agttttactt gtaactgaag
     4801 ataatttaga aggtagtatt atgtcagaag tgtcagcgat tattgcagag cattgcttgt
     4861 tcgatttaga tgcaccaatc atgcgtttag ctgctccaga tgtaccatct atgccatttt
     4921 ctcctgtatt agaaaatgaa attatgatga atccagaaaa aatcttaaat aaaatgcgtg
     4981 aattagcaga attctaggga gggaaagtca tggaaataac aatgcctaag ttaggtgaga
     5041 gtgttcatga aggcaccatt gaacaatggt tagtttctgt tggtgatcat attgatgaat
     5101 atgaaccatt atgtgaagtt attacagata aagtgacagc tgaagtccct tccacgatat
     5161 caggaacaat tacagaaatt ttagttgaag cggggcagac agtagctatt gatacaatta
     5221 tctgtaaaat tgaaactgct gatgaaaaga caaatgaaac aactgaagag atacaagcaa
     5281 aagtggatga gcatactcag aaatctacta aaaaagctag tgcaacagtg gaacagacat
     5341 ctactgctaa acaaaatcaa ccacgtaata atggtcgctt ttcacctgtt gtatttaaac
     5401 tcgcttcaga gcatgacatt gatttatcac aagttgtagg tagtggattt gaaggtcgtg
     5461 taactaagaa ggatataatg tcagttattg aaaatggtgg taccacagct caatctgaca
     5521 aacaagttca aacaaaatca acatcagtag atacatcaag taaccaatca tctgaagaca
     5581 atagtgaaaa cagcacaata ccagtaaatg gtgtgcgtaa
//
LOCUS       DANRDI010000160         5590 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.161, whole genome shotgun sequence.
ACCESSION   DANRDI010000160 DANRDI010000000
VERSION     DANRDI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.161"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(132..536)
                     /locus_tag="PZ377_002374"
     CDS             complement(132..536)
                     /locus_tag="PZ377_002374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDM6359230.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(556..1968)
                     /gene="atpD"
                     /locus_tag="PZ377_002375"
     CDS             complement(556..1968)
                     /gene="atpD"
                     /locus_tag="PZ377_002375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDM6359231.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(1990..2856)
                     /locus_tag="PZ377_002376"
     CDS             complement(1990..2856)
                     /locus_tag="PZ377_002376"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDM6359232.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(2887..4395)
                     /locus_tag="PZ377_002377"
     CDS             complement(2887..4395)
                     /locus_tag="PZ377_002377"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDM6359233.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4417..4956)
                     /locus_tag="PZ377_002378"
     CDS             complement(4417..4956)
                     /locus_tag="PZ377_002378"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDM6359234.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(4956..5477)
                     /locus_tag="PZ377_002379"
     CDS             complement(4956..5477)
                     /locus_tag="PZ377_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDM6359235.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
BASE COUNT         1570 a         1144 c          875 g         2001 t
ORIGIN      
        1 gaaccactac atcataaatc tttaatggtt ctttatcaat tccttccccc ctctcatgtt
       61 ttgtatcttt aaatcaatat aattcaaaaa agggtcgaag atatgaaatc acatcttcga
      121 ccctttattt actattttaa ttcagccaca cgcaaacggt tatttgctct ctctaaagct
      181 ctttcggctc tatgaatatc agtattgtcg tcatcatttt ccaagtgaga ctctgctctt
      241 gctttggcta atttagctct ttcaacatca atttctcttg cagtttctgc agtctgaaca
      301 atgattgaaa ctttatcttt tctaacttca acaaagccat cgcttacagc aatatattca
      361 gttccatcgt gaaatttcac ttttacaaag cctgttttta aagcagctac agttggaata
      421 tgtccactca tgacacctat ctcaccagct gttgtttgca taacaacgag ttcaacatta
      481 tcacgattgt aaacagaacc attaggagtg acaatatcta ggtttaatgt attcattatc
      541 cattcctcct aattgttata cttcaacacc catatcttta gcttttgcaa taacatcatc
      601 catgctacca actaaacgga atgcatcttc tggaatatgg tcatatttac catctaagat
      661 atctttaaag tttgcaactg ttgtcttaac aggtacataa gaaccttttt gaccagtaaa
      721 ttgttccgct acgtggaagt tttgagataa gaagaattga attctacgtg cgcgttcaac
      781 tgtttgttta tcttcatcag ataattcgtc catacctaag atagcaatga tatcttgtaa
      841 ttcacggtat ttttgaagtg ttgattgtac atcacgagct acttcataat gttcttgacc
      901 tacaattgat ggttccaatg ctcttgatgt agacgctaat ggatccacgg ctggataaat
      961 acccatttca gttaatttac gttctaagtt tgtagttgca tctaaatggg caaacgctgt
     1021 cgcaggcgct gggtcagtat agtcatcggc aggtacgaat accgcttgaa tagaagtaac
     1081 tgatcctttt gttgtagacg taatacgttc ttgtaattgt cccatttcag tagcaagtgt
     1141 tggttggtaa cctactgcag aaggcatacg acctaataat gcagatacct cagaaccagc
     1201 ttgtgtaaat ctgaaaatgt tatcgatgaa taataatacg tcttgacctt gttcgtcacg
     1261 gaaatattca gccattgtta aaccagataa tgcaacacgc atacgtgcac caggtggctc
     1321 attcatttgc ccgaatacca tggctgtttt cttaattaca ccactgtcac tcatttcgaa
     1381 gtataaatcg ttaccttcac gagtacgttc acctacaccg gcgaatacag aaataccacc
     1441 gtgctcttga gcgatgttgt taattaattc ttggattaat actgttttac ctacaccggc
     1501 accaccgaac aatccgattt taccaccttt aatataaggt gctagtaaat ctactacttt
     1561 aatacctgtt tctaaaattt gaacttctgt tgaaagttca tcgaatgctg gtgcttgacg
     1621 atggatagga tcgcggcgaa cagaatcact aatttcttct ttaaggtcaa ttgtttcacc
     1681 tagtacatta aatacacgac ctaatgtttc gtcaccaaca ggtacactaa tttctttgcc
     1741 tgtatctttt acatccatgc ctctttggac accatcagtt gaatccatcg caattgtacg
     1801 aacaacgtcg tcacctaatt gcagcgcaac ttctaatgtt agttgtattg taccttcttc
     1861 tttaggcaca tcaataacca aggcgttatt aattttagga acttcgttat gttcaaatcg
     1921 aacatcaatt acaggaccca taacttgagt tacacggcca attcccatgc tattttcctc
     1981 ctttaaatat tattcaagcg ctgcggaacc accaacaatt tcagtaattt gttgcgtaat
     2041 ttctgcttgt ctcgctctgt tatattctaa tgataagtca tcaataagtt cagttgcatt
     2101 atcagtggca tttttcatcg cagtcatacg tgttgcatgc tcacttgctt ttgcgtctaa
     2161 tattgttccg taaatcaaac tctcaacata ttgaggcaag attacactta agatagattc
     2221 tttatctggc tcaaattcat aagaagacaa atgaccatgc cccttactag aatcctcttg
     2281 agataatggt aatacttgtc tagatgtagg cttgttttca agaacgctga cataatgact
     2341 atagtatata tttaattcat caatttcttc ttcactgtat aagtctatag catggttagc
     2401 tagtgcttga acagatttga aagaaggttg atcaggtacg tctacttgag aatactcaat
     2461 gtcataacct ctatttttta ggaaatcaac accttgttgc cctaaaacta caatactgta
     2521 ttcgctacta tcttgatgtt tcgcttcaat atcagtaatc aattttttaa gcacgtttgc
     2581 actatatgca cctgctaaac ctttatcact cgtgataact aaatatccac ttctagtaat
     2641 tttacgaggt cttagcattg gatggtttgt attgctactt gcccctgcaa ctgcagtaat
     2701 tgcatcttgc attttatcca tatatggtgt gaattgtttt gtattttttt cagctctacg
     2761 aagttttgaa cttgatacca tgttcatcgc tttcgtaatc tgcttcattt ttttggttga
     2821 ttttattcga gtatctattt ctttaagaga agccactatc tcaccacctt atactaaact
     2881 tgtttattat tcagatttgc taaagctttt tttgaattcg ttaattgctg tgtcaaactt
     2941 ctcagcatct ggtaagccac cagtttctct gatttcattt aacagttcag tagcatttga
     3001 ttctgcccag tggtttaact cgtcttcaaa acgtgtgata tctacaactg gaatatcatc
     3061 taaatatcct tttgttaatg cataaataat caacacttgg tgttcgactg gtaatggttt
     3121 gttttgatct tgttttaaga cttcaacagt acgtttacca cgttctaatt tacttgcagt
     3181 aaattcatca aggtctgaac cgaattgtgc aaatgattca agttctctgt atgacgctaa
     3241 gtcaagacgt aacgtaccag caactttctt cattgcttta atttgtgcag atccaccaac
     3301 acgagataca gattgtccgg cattaatcgc tggtcttaca cctgagaaga ataaatcaga
     3361 ttgtaagaag atttgtccat ctgtaattga aataacgttt gttggtacat aagctgaaat
     3421 atcaccagct tgtgtttcga taattggtaa tgcagtaatt gaaccgccac ctaagtcatc
     3481 gtttaatttt gctgctcttt ctaataatct actatgtaag tagaatacgt cacctgggta
     3541 tgcttcacga cctggaggtc tacgtaataa taatgataac tcacgataag ctgctgcttg
     3601 tttagttaaa tcatcataaa cgattaaaac atgtttaccg ttaaacatga attcttcacc
     3661 cattgttaca cctgaatatg gtgcaatata taataatgga gaaggttcag aagctgatgc
     3721 tgctacaaca atagtgtagt ctaaagcgcc tgcttgtctt aacttttcaa catttgctct
     3781 tactgttgaa tctttttgac caatagcaac atagatacaa atcgtacctt gatctttttg
     3841 gttcaaaatt gtgtcaattg caattgttgt tttacctgtt tgacggtcac cgatgattaa
     3901 ctcacgttga cctctaccaa ttggtactaa agcatcaatt gctttgatac ctgtttgtaa
     3961 tggctcatct actgatttac gatccattac accagtagct tttttctcta ctggacgtgt
     4021 tttagttgtg ttaatcggtc cttgtccatc aataggttgt cctaatggat taacaactct
     4081 tccgattagt tcttcaccta ctggtacttc catgatacga ccagtacgtt taacttcgtc
     4141 accttcagta atacctgtgt atggtcctaa aataaccaca cccacgtttg actcttcaag
     4201 gttttgggct aaaccaagta cgccgttatg gaattctact agctcaccag ccataacgtc
     4261 atttaatccg tgaattaatg caataccatc accaatttgt aatacagtac ctacatcagt
     4321 tacggacatt tctgactcat aattttcaat ttgtgagcga agtaatgcac tgatttcttc
     4381 agcttttatg gccatctatg tcactcctct ttataattaa ttaactcttc taaattttct
     4441 ttgtaattgg acaagatcat ttctaacact accatctaat acagttgtgc cgactttaac
     4501 tctaaatcca ccaattaaat ctggattaat ttttgtatct acaataactt tagataactt
     4561 cgtttgttga gttactagtt tgacaatctt atctaactct tcttgactca attcatatgt
     4621 tgactcaatt gttgcaaaat cttgattgta gtgtccgtta tataagcttt ggaacgcctt
     4681 gaatacgtca gctattaatg agatatgtct attatctgct aacacgtaca tcatattttt
     4741 aatgtatgga ttaatgtcag taaatacacc attaatcaac tcacgacgtt gttctgctgt
     4801 ttgagttgga ttactgtcaa ccattctcaa ttgctcaatt ttatctttta ctgcttcatt
     4861 tataactgtt aattcttcat taatagtctc taaattattt gtatctaatg acacgtcaaa
     4921 taatgcttta gcatacttgt tagctacttt taccattatt tatcgcctgc ctcttttaga
     4981 tacttgtcaa ccaatgcttt ttggtcttgt tcagaaattt cttttctaag aactttagaa
     5041 gcaattaaca ctgatagttc agatacttga ttattaatat ctgcaatggc acgttctttt
     5101 tggctattga tttcactttg tgctgtttca atcataccgt ttgcacgtac gtttgcttca
     5161 tgaataattt gttcttgctg ttgacgtgct tgaaccttag catcttctaa aatcttttga
     5221 acttcttctt gtgtttcttt aagtttttgt ttattttctt cttcaagttt ctgtgcattt
     5281 aacttagctt gttctgcgtc atcgatatct ctgttaatat ctctttcacg tttatccatt
     5341 acatctttca atggacccca tgcgaacttt ttaagtaacg caagtaacac gatgaaagtt
     5401 aggacctgta caatcacagt accccactca acgcctccag ctgcaccaag aacgaataag
     5461 ttagctgttt cagtcacggg ttcatgacac tcctttcatt aaaataatcg ctagtgttat
     5521 atagaaaaaa acgacgaggt taggacctac tgaatttatg cgataggcat ggtgcgttca
     5581 tatctcaact
//
LOCUS       DANRDI010000161         5586 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.162, whole genome shotgun sequence.
ACCESSION   DANRDI010000161 DANRDI010000000
VERSION     DANRDI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.162"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..201
                     /locus_tag="PZ377_002380"
     CDS             <1..201
                     /locus_tag="PZ377_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 family protein"
                     /protein_id="HDM6359236.1"
                     /translation="SLRTQEKIYDKLDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWI
                     LGLIGTILSTFVIALLKTIFGI"
     gene            337..639
                     /locus_tag="PZ377_002381"
     CDS             337..639
                     /locus_tag="PZ377_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDM6359237.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            650..2104
                     /locus_tag="PZ377_002382"
     CDS             650..2104
                     /locus_tag="PZ377_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDM6359238.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
     gene            2494..3432
                     /gene="lukS-PV"
                     /locus_tag="PZ377_002383"
     CDS             2494..3432
                     /gene="lukS-PV"
                     /locus_tag="PZ377_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HDM6359239.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            3428..4411
                     /gene="lukF-PV"
                     /locus_tag="PZ377_002384"
     CDS             3428..4411
                     /gene="lukF-PV"
                     /locus_tag="PZ377_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HDM6359240.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            4660..4845
                     /locus_tag="PZ377_002385"
     CDS             4660..4845
                     /locus_tag="PZ377_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359241.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            4936..>5586
                     /locus_tag="PZ377_002386"
     CDS             4936..>5586
                     /locus_tag="PZ377_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDM6359242.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFK"
BASE COUNT         2173 a          748 c         1006 g         1659 t
ORIGIN      
        1 agtctgagaa cgcaagaaaa aatttatgac aagttagata gaaatttcga agaactaagg
       61 cgtgacaaag aagaagatga aaaaaataaa gagaaaaatg ctaaaaatat tagagacatc
      121 aagatgtgga ttctaggatt aatagggacg attctaagta catttgttat agccttgtta
      181 aaaactattt ttggcattta aaggaggtga tcaccatgct taagggaatt ttaggatata
      241 gcttttggtc gtgtttctgg tttggtaagt gtaagtaata tttaagagtc agtgcttcgg
      301 cactggcttt ttattttgga taaaaggagc aaacaaatgg atgcaaaagt aataacaaga
      361 tacatcgtat tgatcttagc attagtaaat caattcttag cgaataaagg tataagtccg
      421 ataccagtag atgaagaaag tgtttcatcg attatcttaa cagttgttgc tttatatact
      481 acatataaag ataatccaac atctcaagaa gggaaatggg cgaatcaaaa attaaagaaa
      541 tataaagctg aaagtaaata tagaaaagca acagggcaag cgccaattaa agaagtaatg
      601 acacctacga atatgaacga cacaaatgat ttagggtagg tgttgaccaa tgttgataac
      661 aaaaaaccaa gcagaaaaat ggtttgataa ttcattaggg aagcagttca atcctgattt
      721 gttttatgga tttcagtgtt atgattacgc caatatgttc tttatgttag cgacaggcga
      781 aaggctgcaa ggtttatatg cttataatat cccgtttgat aataaagcaa agattgaaaa
      841 atatggtcaa ataattaaaa actatgacag ctttttaccg caaaagttgg atattgtcgt
      901 tttcccgtca aagtatggtg gcggagctgg acacgttgaa attgttgaga gcgcaaattt
      961 aaacactttc acatcatttg gtcaaaactg gaacggtaaa ggttggacta atggcgttgc
     1021 gcaacctggt tggggtcctg aaactgtgac aagacatgtt cattattatg acaatccaat
     1081 gtattttatt aggttaaact tccctaacaa cttaagcgtt ggcaataaag ctaaaggtat
     1141 tattaagcaa gcgactacaa aaaaagaggc agtaattaaa cctaaaaaaa ttatgcttgt
     1201 agccggtcat ggttataacg atcctggagc agtaggaaac ggaacaaacg aacgcgattt
     1261 tatacgtaaa tatataacgc ctaatatcgc taagtattta agacatgcag gacatgaagt
     1321 tgcattatac ggtggctcaa gtcaatcaca agatatgtat caagatactg catacggtgt
     1381 taatgtaggc aataaaaaag attatggctt atattgggtt aaatcacagg ggtatgacat
     1441 tgttctagaa atacatttag acgcagcagg agaaagcgca agtggtgggc atgttattat
     1501 ctcaagtcaa ttcaatgcag atactattga taaaagtata caagatgtta ttaaaaataa
     1561 tttaggacaa ataagaggtg taacacctcg taacgattta ctaaatgtta atgtatcagc
     1621 agaaataaat atcaattatc gcttatctga attaggtttt attactaata aaaatgatat
     1681 ggattggatt aagaaaaatt acgacttgta ctctaaacta atagctggtg cgattcatgg
     1741 taagccaatt ggtggagtgg tagctagtga ggtcaaagcg ccagttaaaa acgaaaagaa
     1801 tccgccagtg ccagcaggtt atacactcga taagaataat gtcccttata aaaaagaaca
     1861 aggcaattac acagtagcta atgttaaagg taataatgta agagacggtt attcaactaa
     1921 ttcaagaatt acaggggtat tacccaacaa cacaacaatt acgtatgacg gtgcatattg
     1981 tattaatggt tatagatgga ttacttatat tgctaatagt ggacaacgtc gctatattgc
     2041 gaccggagag gtagacatag caggcaaccg aataagcagt tttggtaagt ttagtgcagt
     2101 ttgataattg tatatgatga atcttaggca ggtacttcgg tacttgccta ttatttaaaa
     2161 ttaataaaca gttaattttt acatgaatat attaaatttt aaaaaaacaa acgtttttag
     2221 tatataaatt attttgtgtt cgtattgtgt gctatgatta aaaagttgtt atggtcaact
     2281 atatcgtggt tttatgttta ttatcaatca aaatataaat tatttataat ttgtttggta
     2341 atgaacgggt ttttttcgaa ataatagtaa aaaaacacat ttgtagatat tttaaactcg
     2401 gtaaatcttt taataaatat ttaattttat taaaagttaa aaaggtttaa tataaaaatg
     2461 taataaaatt tataaagaaa ggaaatgatt tttatggtca aaaaaagact attagctgca
     2521 acattgtcgt taggaataat cactcctatt gctacttcgt ttcatgaatc taaagctgat
     2581 aacaatattg agaatattgg tgatggcgct gaggtagtca aaagaacaga agatacaagt
     2641 agcgataagt ggggggtcac acaaaatatt cagtttgatt ttgttaaaga taaaaagtat
     2701 aacaaagacg ctttgatttt aaaaatgcaa ggttttatca attcaaagac tacttattac
     2761 aattacaaaa acacagatca tataaaagca atgaggtggc ctttccaata caatattggt
     2821 ctcaaaacaa atgaccccaa tgtagattta ataaattatc tacctaaaaa taaaatagat
     2881 tcagtaaatg ttagtcaaac attaggttat aacataggtg gtaattttaa tagtggtcca
     2941 tcaacaggag gtaatggttc atttaattat tcaaaaacaa ttagttataa tcaacaaaac
     3001 tatatcagtg aagtagaacg tcaaaattca aaaagtgttc aatggggaat aaaagctaat
     3061 tcatttatca catcattagg taaaatgtct ggacatgatc caaatttatt tgttggatat
     3121 aaaccatata gtcaaaatcc gagagactat tttgttccag acaatgaatt acccccatta
     3181 gtacacagtg gtttcaatcc ttcatttatt gcaactgttt ctcatgaaaa aggctcagga
     3241 gatacaagtg aatttgaaat aacgtatggc agaaatatgg atgttactca tgctactaga
     3301 agaacaacac actatggcaa tagttattta gaaggatcta gaatacacaa cgcatttgta
     3361 aacagaaatt acacagttaa atatgaagtg aactggaaaa ctcatgaaat taaagtgaaa
     3421 ggacataatt gatatgaaaa aaatagtcaa atcatcagtt gttacatcaa ttgcattgct
     3481 tttgctatcc aatacagttg atgcagctca acatatcaca cctgtaagtg agaaaaaggt
     3541 tgatgataaa attactttgt acaaaacaac tgcaacatca gattccgata agttaaaaat
     3601 ttctcagatt ttaactttta attttattaa agataaaagt tatgataaag atacattaat
     3661 actcaaagct gctggaaaca tttattctgg ctatacaaag ccaaatccaa aagacactat
     3721 tagttctcaa ttttattggg gttctaagta caacatttca attaattcag attctaatga
     3781 ctcagtaaac gttgtagatt atgcacctaa aaatcaaaat gaagaatttc aagtacaaca
     3841 aacggtaggt tattcttatg gtggagatat taatatctct aacggcttat caggtggagg
     3901 taatggttca aaatcttttt cagagacaat taactataaa caagaaagct atagaactag
     3961 cttagataaa agaactaatt tcaaaaaaat tggttgggat gttgaagcac ataaaattat
     4021 gaataatggt tggggaccat atggcagaga tagttatcat tcaacttatg gtaatgaaat
     4081 gtttttaggc tcaagacaaa gcaacttaaa tgctggacaa aacttcttgg aatatcacaa
     4141 aatgccagtg ttatccagag gtaacttcaa tccagaattt attggtgtcc tatctcgaaa
     4201 acaaaacgct gcaaaaaaat cgaaaattac tgttacttat caaagagaaa tggatagata
     4261 tacaaacttt tggaatcaac ttcactggat aggtaataat tataaagatg aaaatagagc
     4321 aactcataca tcaatttatg aagttgattg ggaaaatcat acagttaaat taatagatac
     4381 tcaatctaag gaaaaaaatc ctatgagcta aacagataga taatcaaaaa atcttaaata
     4441 tgttaaaatt tacaaacact ttctttctat attagggtaa ccacgtctta attgacgtgg
     4501 ttattttttc agggcaaaaa aagggcggat tatttaaata aggacaaaca cttgtggaaa
     4561 atttaaaagg ttaaaaataa taaagaactt ggtataacaa gggttttata catttgcgta
     4621 caacgacgaa atgtcaattt accatcacat tatgacgata tgtttatttt aaacacacaa
     4681 gctcatgcgc gtcttgatca aatggcacaa cagtttgaag ttgtttgtaa tggcttgaac
     4741 gaaaatgaag gacaagcaat tcaaacgatg gatcaatctg cctctctaat acggtcaaac
     4801 ttaattcaag ttaaagaaca attagaaaaa ctagctgtat actaattaat ttattaaatg
     4861 ctacttgttt tcattgagaa taagtagctt tttcaaacat aaaagtttta caaacacata
     4921 aatgggtgat gagctatgtt taaaagaact aaactaatct taatagcaac attactgcta
     4981 tcaggatgtt caactaccaa taacgaatct aataaagaaa caaaatcagt gccagaagaa
     5041 atggaagctt caaaatatgt aggtcaaggc ttccaaccac ctgcagaaaa agatgtggtt
     5101 gaatttgcga aaaagcataa agataaaatt gccaaacgag gcgaacaatt ttttatggat
     5161 aatttcggtc taaaagttaa agctaccaat gttgtaggta gtggaaaagg cgtagaagta
     5221 ttcgtgcatt gtgatgacca cgatatcgta tttaatgcga gtattccatt tgataaatca
     5281 ataattgaga gtgatagctc attaagaagt gaggacaaag gcgatgatat gagtacttta
     5341 gttggtacag tgttgagtgg ctttgaatat cgaacacaaa aagaaaagta tgacaattta
     5401 tataaatttt tcaaagataa tgaagagaaa taccaatata caggctttac aaaagaagca
     5461 ataaacaaga cgcaaaatgt cggatataaa aatgaatatt tttatatcac ttactcgtca
     5521 agaagcttaa aagaatatcg taagtattac gaaccattga ttcataaaaa tgataaagaa
     5581 tttaaa
//
LOCUS       DANRDI010000162         5575 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.163, whole genome shotgun sequence.
ACCESSION   DANRDI010000162 DANRDI010000000
VERSION     DANRDI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.163"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            527..1624
                     /locus_tag="PZ377_002387"
     CDS             527..1624
                     /locus_tag="PZ377_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDM6359243.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMQNTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNIS
                     NSFKKKINLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGREL
                     VELHLNYEKQLNWDGVEIIYNNMNPNYKVEK"
     gene            1645..1914
                     /locus_tag="PZ377_002388"
     CDS             1645..1914
                     /locus_tag="PZ377_002388"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDM6359244.1"
                     /translation="MDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQYNVSIDKK
                     SGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            1975..2718
                     /locus_tag="PZ377_002389"
     CDS             1975..2718
                     /locus_tag="PZ377_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359245.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            2719..3855
                     /locus_tag="PZ377_002390"
     CDS             2719..3855
                     /locus_tag="PZ377_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDM6359246.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSL
                     ENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKV
                     SLSKDLVDRETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSN
                     FNSIEEAIVNIGEPVFSRIDSCIKFLYLTQEEKQKVIENKLNEILSSLNEKEKRIVTA
                     YNLLEKYKETEIDIDNIRYLKKIITNDIYTIIFEDELFNTDIID"
     gene            3984..5390
                     /locus_tag="PZ377_002391"
     CDS             3984..5390
                     /locus_tag="PZ377_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359247.1"
                     /translation="MNNSNFLNVELIGTKIITMEDFYKMKEEEFFLKSKLPLYLYSFN
                     YVDEYTMKLLDRGRISILNTKTNKDCIFDISKYIHENIIFRRDIFELNDIRDDDLKYK
                     INGYNIEVYLSGELEKIVKHLFENKFDVEYGFYILFIKPIFEIVFSSNYVKEEFNTKI
                     ESSEYRESLDSFNEILMKYLENEINFKEVWKKYIDELFKKLNVQKLLIHKKIESLISS
                     YDKKISDDCSMYSKVFEQYKKNVVLDKVYYSEVYKYSDNLIKVFSEDVWNIMGYKKYN
                     EYFTNRDDTTLKITNLYIGQSFGTEGNRNVIDRIGKGHEKLQEIMTYQPENRKTVLIF
                     YKIQPDNIYITNQYGKEAVSRLKRILEQSIPNKEYIDLSEISLITFFKPEYNKQHVNE
                     EFNSLSSSKIKNLAKENDGIIIYLNFKDVNYQLISEGNTFFSAKPYLHCVFNKDILNM
                     DKVDIENFFKSIEVKYSK"
BASE COUNT         2304 a          564 c          903 g         1804 t
ORIGIN      
        1 aaaacacctc gatattatgt cgaggtgttt ttttgcgttt ttgcggcgaa tatggaatgt
       61 gtagaatata aatgaatttt taccttccca ccataaaaga tgaagaacca tgaaatgtgg
      121 agaacaataa atagttgata ttctgttatt tttttggaag tgaagtgatt tgaatacttt
      181 actctaaacg ataaaaggtt aaaaaaacaa caaaaagaaa atattactgt cataattgga
      241 aatacaccgt attctgctgg tcaatctagt gtaaataatg ataataaaaa aatgaatatg
      301 aaaaattaaa tgcaaaaata gcaaatacct atgcaaattt ttcaacagca aatttaaaaa
      361 aatcattata tgattcgtat atacaggctt ttcgatgggc aactgataga gttggaaaaa
      421 aggtattata ggatttgtaa caaatggatc atttattgat agtcaaagca ctgatggatt
      481 tagaaaagtt ttatacgatg aatttaacta tctgtacata attaatttga gaggcgacca
      541 acgaacacag ggtgaaaaat caagaaaaga aggtggaaaa atatttggat caggaagtag
      601 agcaccgata gctatatcta ttttagttaa agatggaagt tacaatcacg atatctatta
      661 taatgacatt ggtgaatatt taactagaga gcaaaaatta gatactttaa tgaaacatca
      721 atcaattgtg aatttgaaaa gtttgaatgt tttacccgat aaaaacaatg attggattaa
      781 tcaacgagac ataaactatg agaattattt accaatgtat gattccaagg atattgaaaa
      841 ttctatatat ttagatcaat ttaatggagt aaattcagca agagataatt gggtaacaaa
      901 tttttcgaat gaaaaagcat tagtaaatgc aaaattactt gtagataact ataattctga
      961 aatagataga ttaattgata tcttagattc aagagaacgt ataaatttag tgaacaaaga
     1021 tgaaacattt attagttgga cacgaggact tacccaaaaa ttttctaaag gaaaaaatat
     1081 ttcaattaat ccagagagaa ttgttaaatt tatgcataga ccatttacaa aaaaatggat
     1141 agtgtacgac aaaaatatta tggaaatgcc aagtagatat tataatataa tgcagaatac
     1201 tggacaggtg atttatatac aaggacaggg aatgaataaa gaattctcgg ctatgatcac
     1261 ggacatatta cctaatttcc aatttattgg taatggcaaa ggctttgcta catacaaagg
     1321 taaagatagt ttgaggcttg tagataatat ttctaattct tttaaaaaga aaataaatct
     1381 taattcagaa gagatagtat attatatata tgcaattttg catcataaat attatgtgaa
     1441 caaatattca agtgatttga gtaaaggttt tccgcgaata ccgatattaa aggatgtata
     1501 tggatttgtt gaaattggta gggagttagt ggaattacat ttaaattatg aaaaacaact
     1561 caattgggat ggtgtagaaa taatttataa taatatgaat ccgaattata aagttgaaaa
     1621 atgaagcacc ctaaaaaggg cgtgttggat acagtaatat acaataccga tattacagtt
     1681 actaacattc caaataaagt atataattat attgtgaatg gcaagcctgc tattgaatgg
     1741 attatagatc agtacaacgt aagcatcgac aagaaatctg gaatattaga tgatcctaat
     1801 gaatttagtg aagatccgaa ttacatttta aatctgcttt tatcagttat tactgtaagt
     1861 atgaaaactc ttggattaat agataaatta ccagattttg aagtattaga ttaaggagag
     1921 gatatattga caaatactat caaaaaaata atttattttg atgaactatc agcaatggat
     1981 ttattacaga tagagaagaa aggtaacctt actaaaacag ttcagttgat gcatgatgaa
     2041 aaaggtagtg caagtggtga atctaaagtg aacatggaat ttggtaagca aagcgctgta
     2101 aaacaaattt ttgattcttt acttgggatg tcagcgagtg cttcggctag tgcaggtata
     2161 aatggaaata taactggaga gcgagtagcc aaaacaattt tagagaatac attaatatat
     2221 gattttttgg atacagtaga gtttagaaag aaaaaaacta ttattgatat ttcagagggg
     2281 tttaaactaa atattcaaca agatacgatg acatatttcg cgactattac ccccatagca
     2341 agtatgtttg aaggatctaa acatctagac gaggaggagc tatcatttga tgtttcaaaa
     2401 atgaatgaag cgattagaag tattaaaggc tattttgaat taataggaac aagagggaat
     2461 gaaaacagag tttttagatt taatatagaa tcttttaaaa ataactatag aatacaagat
     2521 ttaaaattta tgaatttaac actctactca gtacttgttg gtgaaataag caaagaaaat
     2581 ctaaattacg ataaagagtt tgatctaaat caaggtgcca aacctagtgg aattggaaag
     2641 tttaagggaa ttagatcaga agcaactaaa gaagataaat atcaagtgta tgatgttatt
     2701 cttgcaggag tgaaataaat ggaaattcat gtttatacgg gaccgctttg tggatttaat
     2761 gaatatatta attctgaagt taaagaatat caccgcttca tggaattaat cagagaatac
     2821 aatatcatag tgcgagcaag tgatacattt ggaactcatg atgttgatgg tatttttgat
     2881 actgagatag aaaatcttgt gatattttca aacgactttg cttctgtaac aactcatgtt
     2941 attactaatt ttataaatat cgttacttta ggtagaaata ttaagaaaat ctatattcag
     3001 aatcctccaa aaagagtatt tcaatcacta aatagttttc acgattctga aataatagaa
     3061 ataaactatg aatatacaac attaaaaaaa gatgatgtaa taaattttta taaagagcag
     3121 atggctggtt ctaaaataat aggtcaaaca gacgctaaaa aacaaatgag catagattta
     3181 tataaacata cttccttaga aaataataaa ccgaccgtaa tgctttacta tggtccatca
     3241 ggtgttggaa aaacagaatt agccaaagaa atagcaaaaa aatataaagg taatatcact
     3301 agaatacaat tttctatgat gcagaatgat gaatctttta attatatctt tggagatgaa
     3361 catgccaaag tatcattgtc aaaagattta gtagatcgtg aaactaatgt tgttttaatt
     3421 gatgaatttg ataaggtatt accaatctat tacaatgttt tctatcaaat gtttgatgaa
     3481 ggtattattg aagatgccaa ttataaagta gacgtttcaa attgtatttt cattctaaca
     3541 tctaatttta atagtattga agaagcaata gttaatattg gagaaccggt attttctaga
     3601 atagattcct gtattaaatt tttatattta acacaggaag aaaagcagaa agtaatagaa
     3661 aataaattaa atgaaatact atcatctcta aatgaaaaag aaaaaagaat agttaccgca
     3721 tataacttgt tagagaaata taaagagaca gaaatagata tcgataacat taggtattta
     3781 aagaaaataa taacaaatga tatttataca ataatttttg aagatgaatt atttaatacg
     3841 gatattatag attaatttat atgctttata tttttttagt ataagttatc tattaataaa
     3901 gtactatcta tgtaaggttg taagtagtat aagttaagag tttaataatt ttaaaattta
     3961 attaatatat aaaggtaaat taaatgaata atagtaattt tttaaatgta gagttaatag
     4021 gtactaaaat aataacaatg gaagactttt ataaaatgaa ggaagaagaa tttttcttaa
     4081 aatctaagct acctttatat ttatattcct tcaactatgt agatgaatat acaatgaaat
     4141 tacttgatag aggaagaata tctatattaa atacgaaaac aaataaagat tgtatatttg
     4201 acatttcaaa gtatatacat gagaatatta tattcagaag agatatattt gaattaaatg
     4261 atataagaga tgatgatttg aagtataaaa ttaatggtta caatattgag gtatacttaa
     4321 gtggtgaatt ggagaaaatt gtaaaacatt tattcgaaaa caagtttgat gtagagtatg
     4381 gtttttatat tttatttatt aaaccaatat ttgaaattgt tttttcttct aactatgtta
     4441 aagaagaatt caatacaaaa atcgaatcat cagaatatag agagagctta gatagtttta
     4501 atgaaatttt aatgaaatat ctagaaaatg aaataaattt caaagaagta tggaaaaaat
     4561 atattgatga attatttaaa aaattaaatg ttcaaaaatt gttaattcat aaaaaaatag
     4621 aaagtttaat atcctcgtat gataaaaaaa tatctgatga ttgtagtatg tactcaaaag
     4681 tatttgaaca atataaaaaa aacgttgtat tggataaagt atattactct gaagtttata
     4741 aatatagtga taatttgata aaagtattct ctgaagacgt ttggaacata atggggtata
     4801 aaaaatataa tgaatatttt acaaacagag atgatactac attaaaaatt actaatttat
     4861 atattgggca atcttttggt acagagggta atagaaatgt cattgataga atagggaaag
     4921 gtcatgaaaa attacaagaa attatgactt atcagccaga aaatagaaag actgtcttaa
     4981 tattttacaa gatacagcct gacaatattt atattacaaa ccaatatggt aaagaggctg
     5041 tgtctcgatt aaaaagaatt ttagagcaaa gtatacctaa taaagaatat attgatttat
     5101 cagagatatc attaattaca ttttttaaac cagaatataa taaacaacat gtgaatgagg
     5161 agtttaattc tttatcttct tcgaaaataa agaatttagc caaagaaaat gatggtatta
     5221 ttatttattt aaattttaag gatgttaatt atcaactgat aagtgaaggc aatacttttt
     5281 tttctgcaaa accatatttg cattgtgtgt tcaataaaga tattttaaat atggataaag
     5341 ttgatataga aaattttttc aaaagtatag aagtcaaata cagtaaataa gatcaattat
     5401 tgaatccacc gaccagcaga tttttttaaa aaattaatac cccgttaata ccctttgctt
     5461 caatttgatg aaaatcaatg aaattcaaaa ttgaagaaat cctttaatat caaggtgtac
     5521 gacagtctat ataatcatgc gaaattctaa aattttctga acgtaaaaaa acaaa
//
LOCUS       DANRDI010000163         5540 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.164, whole genome shotgun sequence.
ACCESSION   DANRDI010000163 DANRDI010000000
VERSION     DANRDI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.164"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            199..585
                     /locus_tag="PZ377_002392"
     CDS             199..585
                     /locus_tag="PZ377_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDM6359248.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            578..874
                     /locus_tag="PZ377_002393"
     CDS             578..874
                     /locus_tag="PZ377_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDM6359249.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      932..1035
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1124..2149
                     /locus_tag="PZ377_002394"
     CDS             1124..2149
                     /locus_tag="PZ377_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDM6359250.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            2142..2837
                     /locus_tag="PZ377_002395"
     CDS             2142..2837
                     /locus_tag="PZ377_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6359251.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            2855..3676
                     /locus_tag="PZ377_002396"
     CDS             2855..3676
                     /locus_tag="PZ377_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDM6359252.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(3819..5039)
                     /locus_tag="PZ377_002397"
     CDS             complement(3819..5039)
                     /locus_tag="PZ377_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDM6359253.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(5127..>5540)
                     /gene="sek"
                     /locus_tag="PZ377_002398"
     CDS             complement(5127..>5540)
                     /gene="sek"
                     /locus_tag="PZ377_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HDM6359254.1"
                     /translation="TATNEYLDKPRNIPINIWINGNHKTISTNKVSTNKKFVTAQEID
                     VKLRKYLQEEYNIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLNNNDTFSYDLFYTG
                     DDGLPKSFLKIYEDNKTVESEKFHLDVDISYKETI"
BASE COUNT         1872 a          807 c          955 g         1906 t
ORIGIN      
        1 ataacattgt agattttagg atgttgattt ttgacttagc ctttgtctgc ttatgttaaa
       61 tgttccagct agtcaaacat tgagtttgta attattatat taagctagtt taaatttttt
      121 gtgatttatg ttttattcat accaattttg tgaaatgata cagtgacaga tgtttattga
      181 ttttaaaggg tgatgatgat ggctattgta aataaagtga taattgttga aggaaaatct
      241 gataaaaaaa gggtgcaaca ggttattgca gaaccagtca atattatttg tactcatgga
      301 acaatgagta tagataagct tgatgatatg atagaatcac tgtatgataa acaagttttt
      361 gtattagccg attctgatga cgaaggagat cgaattagaa attggtttaa acgttatttg
      421 agtgaaagtg aacatatatt tattgataaa acttactgtc aagttgcgaa ttgccccaaa
      481 caatatttgg cgcatgtact ttcaaaacat ggctttactt gtaagaaaga aacacctctt
      541 ttaccgaata taaataatga aaggttagtt ttagtaaatg aataattcat tagacatcaa
      601 agatgtaact acattttatg aggaagacaa acatttaatc tttggttata caccaacgtg
      661 tggtacttgt aaggtttcag aaagaatgtt agacattgct aatgaaatat tgcagttacc
      721 attattgaaa atagatttaa acttttatcc tcagttttgt aaagatatgc aaatcatgtc
      781 tacgccgatt ttattgttga tgaataaaga taaagaagta aaacgaattt atgcatttaa
      841 atcggtgact gatttgttag aaaatttaaa atagttattg acgaaatttt actaaggtgt
      901 taggattaat acaaattaaa tgatgcgtaa actcttatcg agagtggtgg agggatgtgc
      961 cctacgaagc ccggcaaccg tcttatatag aaatggtgcc aattcacata aagttttaac
     1021 ttttgaagat gagagaaaca atactactat tgctttctca atttttctat cgatattgag
     1081 aaagcatttt ttattttatt aagcaacaca gggaggaatc aacgtgattg aattaaaaga
     1141 agttgttaaa gaatatcgga ctaaaaataa agaagtcctt gctgtagatc acgttaattt
     1201 atcgattcga gcaggatcga tttatggcgt cattggtttt tctggagcag gaaaaagtac
     1261 tttgattcga atgtttaatc atttagaagc gcctacatca ggtgaagtta ttatagatgg
     1321 agaccatata ggtcaattgt ccaaaaatgg attaagagca aaaagacaaa aagtaagtat
     1381 gatcttccaa cattttaatt tgttatggtc aaggactgtg ttaaaaaata ttatgtttcc
     1441 gcttgaaatt gcaggtgtcc ctagaaggag agctaagcaa aaagcattag aacttgtcga
     1501 actcgtcggt ttaaaaggta gagaaaaggc ttatccatca gagttatcag gtggacaaaa
     1561 gcaacgtgtt gggattgcac gagcgttagc taatgatcca acggtcttgc tttgtgatga
     1621 ggcaacaagt gcacttgatc cgcaaacaac agatgaaatt ttagatctac tactaaaaat
     1681 tagagaacaa caaaatttaa caattgtact aattacgcat gaaatgcatg tcattcgtcg
     1741 tatttgtgat gaagttgcag ttatggaaag tggtaaagtg atagaacaag gaccggtgac
     1801 acaggttttt gaaaatccgc aacacactgt gacaaaacga tttgtgaaag acgatttaaa
     1861 tgatgatttc gaaacatctt taacagaatt agagccatta gaaaaagatg catatatcgt
     1921 tagattagtt ttcgctggtt caacaacaac cgagcctatt gtatcgagtc tatcaactgc
     1981 ctatgatatt aaaattaata ttttagaagc aaatattaaa aatacaaaaa atggaacagt
     2041 cggcttttta gttctgcata ttccatatat ttcaagtgta gatttcggaa aattcgaaaa
     2101 agagttaatt gagcgacaag ttaaaatgga ggtgttaaga catgggtaaa tcatttagtg
     2161 aaattataaa tgaaatgatt acaatgccta atattcagtg gccagaagtt tggactgcaa
     2221 tagtcgaaac actatacatg acagtcgtct caactatatt tgcatttata cttggtctta
     2281 ttttaggtgt gttattattc ttgtctgcta aaggtaagtc tatcggtgca aggttatttt
     2341 attctatcgt ttctttcatt gttaacttat ttagagcgat accatttatt attttaattt
     2401 tattattaat tccatttaca agtttgatac ttggaacgat aagtggtccg acaggtgcgt
     2461 taccagcctt gatcattggc gcagcaccgt tttatgcaag gctcgtagaa attgctttta
     2521 aagaaattga taaaggtgtc atcgaagcgg cttggtcaat gggcgctaat acttggacag
     2581 taattcgtaa agtcctttta cctgaagcta tgccagcgct agtgtctggc attacagtta
     2641 cagcaatcgc tttagttggt tcaacagcag ttgcaggtgt aattggtgcc ggtggtttag
     2701 gaaatttagc atacttaaca ggtttcactc gaaatcaaaa tgatgtcatt ttagtatcaa
     2761 cagtttttat tttaattatt gtatttataa tccaattcat tggggattgg cttacaaata
     2821 aacttgataa acgataaatt gggggtttca ttttatgaaa aaattatttg gtcttatttt
     2881 agtattaaca tttgcagttg tattagcagc ttgcggtaat ggaaacaaaa gtggcagtga
     2941 cgacaagaaa ataacagtag gtgcttcacc agcaccacat gctgaaattt tagaaaaagc
     3001 aaaaccatta ttagagaaaa aaggttatga actagatatt aaaacaatta acgattacac
     3061 tacacctaat aaattactag acaaaggtga aattgacgca aactatttcc aacatacacc
     3121 atatttaaac acagagaaaa aggataaagg ttacaaaatc gtaagtgccg gtgatgttca
     3181 cttagaacct atggctgtat actctaaaaa gtataaaagt ttaaaagaat taccaaaagg
     3241 tgcaacagtc tatgtgtcta ataatccagc tgaacaagga cgtttcttaa aattcttcgt
     3301 tgatgcaggt ttaattaaaa tcaaaaaagg cgtaaaaatt gaagatgcta agtttagtga
     3361 tattacagag aataaaaaag atattaagtt taataataaa caatcagcag aattcttacc
     3421 taaaatttat caaaatgaag acgctgatgc tgttatcatt aattcgaact ttgcaatcga
     3481 acaaaaacta aatcctaaaa aagattctat tgctgtagaa agtgcgaaag ataatcctta
     3541 tgcaaactta attgctgtta aagaaggaca tcaagatgat aagaaaatca aagcattaat
     3601 tgaagtatta caatctaaag atattcaaga cttcattaat gaaaaataca atggtgcagt
     3661 tattcctgct aaataatacc aattaaataa aaataccctt taataacaac gattaagggg
     3721 ttatttttgt ggtattgaaa ataaaaaagg gcacaaaagg ggcagtatat gcaaattatc
     3781 gaattttaaa caaaaaaata cgctcagatt tgagcgcatt atccaagttt tacctgttca
     3841 agtttgttca tcatatcctt atccatctgt tcagtaacgt gagaataaat ggaaagtgtt
     3901 gtacggtggt cggaatggcc tacacgatcc ataatagctt taagtgacac gccttgttga
     3961 gagagtaatg atatgtggct atgtcttaat atatgtgaag aaacttcttt atcaatacca
     4021 acatctttag ctgcttccct aaggatttta ttgaatcttt cagtctgcat tgggttgcct
     4081 ttatgattag taaatacaaa atttctattt agatatccat cattccattt tgaatccttt
     4141 ttgttctcca gtattgcttt ctttaatatc tcgcaacttc tgctactcaa tccaattgtt
     4201 ctataactag actctgtttt agtggtatct tttacaccga atccaccaga ttcatcgtga
     4261 aaccaatgga ttgttccgtt aatatttaaa ctcttattat caaagtctat atcttcattt
     4321 tgaatggcta acatttcacc aatacgcata ccatttaagg cttggaattc tgtcatgagt
     4381 gcaacgaata aataaaaccg cttatggata cctgagtgca tcttctgagc cttcgtatta
     4441 atatctttga tgatagattc tatttcattt aattcgaggt acttattacg tttagctttc
     4501 acttcatcat aagacttaat tcttttattt aatgttatat cttttaaaaa ttctaaatct
     4561 tgcagtttat agatacgtct agtgtattca aatacatttt tgaatatgct taacgcatct
     4621 ttgtttactt gatatacata acctttacta tccatttcat cgaaaacttg ttgcgcatat
     4681 gaaagtgtta ttttattgat taaaatatct tcatctacaa atttcttcaa agtgtttagc
     4741 ttactcaatt tagttttaat agttgttctt tttgacccag acgttttgat ataattctga
     4801 aaccactcat cacatgcgac atggaaagtt agtgacttaa gtgtagtagg tttcttatca
     4861 ttcagttttt cttctatacg ctcatttaag agtctttgag cttctttttg tgattgctta
     4921 ccattcttat taagtaccac gctaacacgt ctccatttgg cagtgagagg gtctttgtac
     4981 ttctcgtaat atctatattt agtttcttta ttcttatttt taaatttttc taaccacatt
     5041 ttgcttctcc ctcctcaaaa ttggcaaaaa ataataaggg taggcatgct acccggaaaa
     5101 taatgattat gacataatta atagatttat atcgtttctt tataagaaat atcgacatcc
     5161 aaatggaatt tctcagactc tacagtttta ttgtcttcgt aaatttttaa aaaacttttt
     5221 ggtaacccat catctcctgt gtagaataaa tcatatgaaa aagtgtcatt attatttaaa
     5281 tggaacgtta ctttaccaat attaaaccca gaataaaatt ttgatttatg accatattct
     5341 tctccttttt tagtgccgtt atgtccataa atgttgtatt cttcttgaag gtactttctt
     5401 aatttgacat caatctcttg agcggtaaca aattttttat ttgtcgaaac tttattggta
     5461 gaaatagttt tgtgatttcc attgatccat atatttatag ggatatttct aggtttatct
     5521 agatattcgt tagtagctgt
//
LOCUS       DANRDI010000164         5502 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.165, whole genome shotgun sequence.
ACCESSION   DANRDI010000164 DANRDI010000000
VERSION     DANRDI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.165"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(106..825)
                     /gene="splC"
                     /locus_tag="PZ377_002399"
     CDS             complement(106..825)
                     /gene="splC"
                     /locus_tag="PZ377_002399"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HDM6359255.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            complement(883..1605)
                     /gene="splB"
                     /locus_tag="PZ377_002400"
     CDS             complement(883..1605)
                     /gene="splB"
                     /locus_tag="PZ377_002400"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HDM6359256.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            complement(1730..2437)
                     /gene="splA"
                     /locus_tag="PZ377_002401"
     CDS             complement(1730..2437)
                     /gene="splA"
                     /locus_tag="PZ377_002401"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HDM6359257.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            3401..3967
                     /locus_tag="PZ377_002402"
     CDS             3401..3967
                     /locus_tag="PZ377_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDM6359258.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
     gene            complement(4441..5139)
                     /locus_tag="PZ377_002403"
     CDS             complement(4441..5139)
                     /locus_tag="PZ377_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HDM6359259.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVIDFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(5136..>5502)
                     /locus_tag="PZ377_002404"
     CDS             complement(5136..>5502)
                     /locus_tag="PZ377_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDM6359260.1"
                     /translation="VNAMLLLVTILLMYVVSLPLIPLNFLLTRYFGVFVSILINLVLS
                     VICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNSKYFNDLNALYISIIVS
                     IIIFAIVTFLNNKKSWRLK"
BASE COUNT         1854 a          904 c          723 g         2021 t
ORIGIN      
        1 tttattcatg atgcttttga tatagacgtt tttttatgtt atattcgacc gatgtttttc
       61 attaaaatta ttgaacacat gctcatggtg tatatttaaa tttgtttatt gttcaatgtg
      121 cttttgaata aaatctttga tttgaggcgt aaagtatacg gcaccattat attcagaacc
      181 aatttttcca ataccgccat acaccacacc tatgacctca ttgttagaat ttagaactgg
      241 tgatcctgaa ttcccgggtt caatgtatgc atcaaaattt aaaatattgt ctttgattct
      301 ttttatagtt cctgtagatt caaactgttt aaaactattt tgagcaggta atgggtaacc
      361 aataacttta attttgtcat caactttagc atctttcgca aaattgaatg cttggacatt
      421 ttcattaaaa ttaaagcctt ttggtccacg ttcgactgct tgttcttcaa tattcatgac
      481 agagatgtct tcatcacccg gataatcaga aatgctttta attttatata taccaccatt
      541 tcctttgtca ccgtttggat gggcagtaat tctatcgcca actttataat cttttgatac
      601 atgtttattg gtgataattg tattttttcc aattacaaaa cctgtcgcat ctttaaatga
      661 aacgacgcca ttatatggaa aaatatttgt atctttaact tgcgtaacat tcttctctgc
      721 atttgctatt tgttgtgtct cttcaacgac tgcagcattt attccagtta ctgaggttag
      781 aatggctaat gctgccatgc ttttaatgac tatattttta ttcattttca agctccttta
      841 caattcgaga tatgttgcaa cgctcgttta aagtcaattt gtttatttat ctatgttttc
      901 tgcaatgaat tttttaattt ctggtgtaaa gtagacgcca tatgcatttc tgttatcatc
      961 attttttaca tcagaagcaa aatgaatacc aactaattcg ttgttgctgt ttaatacagg
     1021 tgatccagag tttccgcttt cagtatgcgc tgaatataca atactgctac cttctactga
     1081 catcacaggg ccagttgact catataaaac atatttattt ttgtatgggt gtggataacc
     1141 aatcacttta attcgctcac cagctttagc ccctgccgca tatttgaatg gcgttacatt
     1201 atcattaaaa ttaaagcctt ttggtccacg ttctattgca cgctcttcaa cttgaatgac
     1261 tgatacatct tctttacctg gataattaat aatcttttta atcgaataaa taccaccatt
     1321 acctttatca ctatttggat gtgcagtaat acgatcgccc actttgtaat ttttcgacac
     1381 atgtttattt gttaaaatag tattctttcc aactacaaat ccagttgcac ttttaaaagc
     1441 aactacacca gtatatggaa aaatattagt atctttaact tttgtgacat tattttctgc
     1501 tttggcagtt tgttgtactt cctcaaccaa tgttgttcca atacctgtta cagatgttaa
     1561 aattgttaat gctgctaaac tcttgatgac tacgtttttg ttcattttaa gttcctctct
     1621 tttaattatt ttgatatcta gatacataat tcagtatatt tagttaatta tgtaaccgtt
     1681 ttcttaatca ctctttaaaa aatcaacttc acgaatgaat tgatgatact tatttttcaa
     1741 tattattttg aataaattct tttaattgtg gtgtgaaata aacaccgaaa ttcttttcag
     1801 attcatcttt tccactacct gcatataaaa taccaatcag ttcatgttta gaattcaata
     1861 caggagatcc tgaattacct ggttgtgcat acgcatcaaa ttccataaac gttccactga
     1921 tatggttaat cgttcctgtc gattcaaaca ttttatattt tgtttgtgca ccctttggat
     1981 aaccaataac agaaattcta tctttcactt ttgctccgtc tgcaaatttt gtataactaa
     2041 cgttcttatt aaaattcaaa ccttctgtac ttgtttcatg aacatgaact atcgcaaggt
     2101 cttcttttcc gggatattct acaatgtctt taacgtcgta gtttcctccg cctttacctt
     2161 tactcgaatg atgtgctgat actctatttt taaaaatatc attactttta gcgatatgtt
     2221 tgttagttac gattgtattt ttaccaacaa ctacaccagt accacccaca aatgctacca
     2281 ctgaattgta tggttcctta gttgcatcgg taatttcttt gacattcttt tctgctttgg
     2341 caattgaatg aggctgatta gaaatatttt cagcaaaacc aagagatgtt aaaatagtta
     2401 aagcagttaa acctttaacc attacatttt tattcatatt ttacctcctt ccaaatttat
     2461 ttttatagtt aatgtatcta gatacacatt tactatatac attttaaata aaaatattaa
     2521 ttaaatatta attaaccact tattaacgtt ttattaaatt attttacatg aattgactca
     2581 tttatttaaa tatgcttaaa actcaatatc acgcaattaa aattgacttt ttaggattat
     2641 tttggtctta aatattaatt attgaattaa aattgtaata tttattcgga aattaggtga
     2701 ttgaaaatat atggcggaat ggtgaacaat tcatgtattt ataatctttc actttactat
     2761 gtattgtgtt taacaacatt attagcgatt agttccccta ttatttatga caatatgcag
     2821 tcaatcaatg aaacatgtat gtatgagtat atttggaact agtaatgtat tgttaaatat
     2881 tgaagatgat tgaattatgg aatttttttc gagatatgtt atttcgggga tcagattttg
     2941 cgattttgcc ttggatagag tagttgcgat ttgtaaatac gtttttcagg ttcgattcaa
     3001 tttcgttgtt aagataatat ggattagttt cactgacatg ttgatgcatg tcttttttgt
     3061 tctgataaag tataaaatgt ttacttaaca ttacttagta tgacacctgc taattcaaac
     3121 attatttgag acattctttt caaattaatt ataaattttt acctatagac tagtttgata
     3181 tttatctaca tctcaaaatt ctcatcaaca atctttcaca tccaacattt ttactttagt
     3241 ttttataatt caaaacaaca aaactatgtt aaaaaattat tctatttttt agttaataga
     3301 tagttaatac atttttgata tttagttaat tgttctttta aaaaattatt attatatttt
     3361 cattgtaaac gtttacaata taaaaaaagg agcaataaaa atgaatacaa aatttttagg
     3421 taaaacatta gtagcaagtg ctttagtatt aacaacatta ggaacaggct tacattcttc
     3481 atacttagga ttagatacaa ataaagttgt taaaacggct aaagcagaag aaaaaatgac
     3541 agatggtcaa ttatggaaaa aagttaaaga ttcattacat gattctgata ttattttaag
     3601 taacgagtac gaaacaatca acgttactta tttattaagc aatggatatt ctagcagcgt
     3661 ttctgctcct ggaaatgatg atggaggcca tctaacacaa tctatagact ttaaaggatt
     3721 aaaacaaatt gatttaacta aggaaaatgt ctacgatgac tttaacaaaa agctagatgc
     3781 taaaaataca tggaattctc taaccgagaa gttaaaaggt cttggtttac tacaaaatgg
     3841 ccaaaaagta tctatttatt cttcagatag ctcttctcca gtctcgggta aagtgggaga
     3901 aggagtaact agtggtggcg aaaatactct aactaaacgc ttcatcaaca aaatcacaat
     3961 tgattaaacc ttaaactcaa atttccttaa ttcataaaac attaataact aaccctttct
     4021 tacaaaccac ctagcccata atatattaaa acgtcatttt tatttcaatt agcggaaaat
     4081 aaaaaaacca ttccgcatta actttggcaa gttggaatgg ttaaaaaaat attttagaag
     4141 ccagcgtttg gcttgcttag acgacatgtt cgcgcatgtc gtcttatttt tacatttata
     4201 taataacacg caattcaata aaatgtctaa tcaataaacc tcaattacat tcaaattcaa
     4261 aaacttttca cctaatattc atcgcttaaa taaacattac gtttaattaa acagacattg
     4321 tcaatcgcgt ctaatacccc tgctgtccga aaacaaaatt attttgtatt catatttaac
     4381 tgttctcttt cctccctttt caacgcaact aaatttcttt taccgttaga gccccaatct
     4441 ttaatcttta attgttctac cgctccattt attaaatcct ttaaagagta aaactgctaa
     4501 tagcaacgtg ataataatat agattgccaa tgttaatgta actggtatac tcccttcgat
     4561 aaacatataa acgtaacgtg tagcatatgt gattggtaaa tagaaccacg aatgatctcc
     4621 aagcacttct aatccaaaat aaacgttaaa aataaacatt aaaactccga caacaatagc
     4681 cattacatct ttaatgaaaa tactaaaaat aaaaagtagc agtaatataa ttacattgaa
     4741 aaacaatgat acgcctataa acataagtgt tattttcata tcatgtgaat gccacaataa
     4801 attaattgat gctaatagaa tacatatggc tgtaatagta taagtaaaaa tcattgatgc
     4861 atttaaccaa tttagcctat tagcttttcc taaaatatga ttaaagtgac caatattttc
     4921 ttcaaaatcg ataacttgat agacgtttat agaaattaat agcgatgtaa ttgcattaaa
     4981 actcgctgta aacaaactta tttgtcgacc attccataaa tttacgttta aataccaatt
     5041 tataaataat ataaacaata tggttacaat aatgggtaca aatgtgagcg cttgcttaga
     5101 aaatttcaac ttacatgact ttaattcgtt aattatcatt ttaatcgcca acttttctta
     5161 ttatttaaaa atgttactat cgcgaaaata atgatgctaa caataatcga aatatataag
     5221 gcattgagat cattaaagta ttttgaatta tggtttacaa ctaaaccatt aggtagtata
     5281 ccaagcgtaa taagcgggat tctctgcatt atcccccacg gcaacaccca aaataaactc
     5341 ttcaatgtta aaaacaagac acaaatgact gataatacta agtttattaa tattgatacg
     5401 aacacaccaa agtatcgagt taataaaaag ttgagcggta tcaatggtag agatactaca
     5461 tacatcaaca atattgtcac caataacaac atagcattaa cc
//
LOCUS       DANRDI010000165         5488 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.166, whole genome shotgun sequence.
ACCESSION   DANRDI010000165 DANRDI010000000
VERSION     DANRDI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.166"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            327..419
                     /locus_tag="PZ377_002405"
     CDS             327..419
                     /locus_tag="PZ377_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359261.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
     gene            complement(1124..1981)
                     /locus_tag="PZ377_002406"
     CDS             complement(1124..1981)
                     /locus_tag="PZ377_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDM6359262.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
     gene            complement(2049..2831)
                     /locus_tag="PZ377_002407"
     CDS             complement(2049..2831)
                     /locus_tag="PZ377_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359263.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(3063..4868)
                     /gene="glmS"
                     /locus_tag="PZ377_002408"
     CDS             complement(3063..4868)
                     /gene="glmS"
                     /locus_tag="PZ377_002408"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDM6359264.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
BASE COUNT         1765 a         1021 c          723 g         1979 t
ORIGIN      
        1 gtgtgtaata ctaataaatc acgtgcattt acgctcacta gctttcatct cctcattttt
       61 gcttttcaaa tttattaagt atgaataaaa aatacatccg aacaaacaaa atctctatat
      121 tctaattcga tttacataga ttatacacta aaaaaatgat tctcaatgca aatgaatgtg
      181 tctctcatag tctaaaaact cgtatgtttc gaattttatg attcattgat aatagtacca
      241 gttaaaagaa ctaagattgt aattatcccc ccttaaaatt caagaatata ttttcttaat
      301 ctatcagcaa gaggttatat ttcaaagtgt ttatttttaa aacaacatct aaaagccatt
      361 ttcataataa tgttaaatca ttagaatgta taaagattcc aattaataaa aatagataaa
      421 atgcaataaa gttccaaata cttattatta ttcatcacaa attacgtgat gccctctaca
      481 acactaaatc aaggatgata tttattttat atacattagc attcattgtt tgcttaccca
      541 cattatcctg tttagtcttt tttataactc tagtcttgaa tgtcatattt acaaagaaaa
      601 aaacactaaa atatttaaaa ctaacactat ttattcactt tgttctttta tagcaccttt
      661 tatgtttatt ttcttgtatt caataaatag tatggagatg ataatttaaa gtagatatat
      721 ttgaagtata ctatagagta aattatattg gggaaatatg taaacgaacc ggttgatttt
      781 atagtggatt aacacttcat agagaatata atcaagaaca aaaacagtca ataggtgtga
      841 atttttttaa agacggatct gttgctacta aaaaaagaag tatacaattc aatagttaaa
      901 ccttaaaaca agaaatatta ttcaaaatca atgaatttcc tatcttatta gttttaacaa
      961 tatttattct catagagttt gtccaattaa atatagatga ttcaaatatt tataagcata
     1021 caaaagagca gtaagacatt ttctaataga aaataacttt actgctcttt gtacattact
     1081 catcaactat tgtaaattaa ataataaata actacctatt ttattattca gctaaaaaat
     1141 gctctgctaa atattttgct gcgccatcct cttcattcgt gtatgccgtt acatctgaag
     1201 ttaatgcttg gatttcaggg cgtgcatttt tcatagcaac tgtataatga ccaaattcaa
     1261 acattgctct atcattgtcg ctatctccaa taactaacgt ttcttcttga tgaataccaa
     1321 aatgtgcaat catttcttta atgcctgtac ctttatcagt ttgataagcc attgtttccg
     1381 cattaaatct tgatgaattt gaaacactaa tctgtagttg cacatgattt tgctttaatt
     1441 catctctaaa tgctgttatt ttttctaaat tagaactgaa tagataaatt tttgaatatt
     1501 caccttcagg aaacttagtt acccaatcta tcttaccagc aagcgcatct tgtcttgaag
     1561 accattcact atgacttacg ccattaatag gatcttgact acgaatcata tctcgcatcc
     1621 atgtttcatc ttcttttaaa gaaactctat taccttcaaa aggaaatacc tcataataaa
     1681 tttgttggcg cttagctaaa ttagtaattt gttgcacttg agccaatgat aaaccatgct
     1741 tgaagataat ttctccatct acttctccaa ttgttccatt tgaactaatg atgccattaa
     1801 ccgcaaaatc ttgaggtaca agttgatgta tttcagaatg cgaacgtcct gttgccaaaa
     1861 atactttata tcctttctct ctcaattgat taatgacatc tttcgtatac gttgatgctt
     1921 gattattttc atgtaaaatt gttccatcca tgtccaaaaa tattgctttt acgttatcca
     1981 tattgccctc cactgtccat tttatcttaa ttaaattttg tcattattac ttattatttg
     2041 caaaatattt actctaacat tgccatagaa aatcgattat tccatctttg aactgctaca
     2101 tttttctgga aaaatcgtga catgttttca gaagttaata tgtcttctac agcgccttgt
     2161 tgaatacttt ggccatcttt tagcagtaaa attttggaaa agttagcagt tatttcttca
     2221 ataaagtgcg tcacataaat catcgcaagc gttggatatg aatctgacaa tgagtcaagt
     2281 atacttaaca acgattctcg tgcaataaag tctaaaccag ctgctggctc atctaaaatt
     2341 aaaacctggg gttgccccat taaagctcgt gcaatcatca ctcgttgttt ttcaccggta
     2401 gataaataac caatatattg ttgcgcttta gcaaacattc caactaattt aagtaattga
     2461 tgtgcttcat tacgtatctc atcatcaata tcttgataaa caccaattga tttaaaggca
     2521 ccgcttatca ccacatcgat tactctttca ccctcttgaa acttttccag taaactatga
     2581 gatacaaaac ctatatgttg tcgtacagtc tctgcagaat accctacctt gcctggcatt
     2641 ttaccgaaaa ggttaacagt tccagatgtt gcaggctcat acgcatttaa aatatttaga
     2701 agtgttgtct tgccagcacc attcaaccca tataatatcc atttatcacc tttagcaatt
     2761 tgccaagaaa tcttttttaa aattgttttt ccttgcttca ttcgcccaat ttgatctaat
     2821 tgaattaaca tgcgaggtca cccctttatt gctactccat acttctcata aaatcatatt
     2881 aacataacac ccttaattgt cagactattt aaataaataa aacacttcat ttttacgcat
     2941 ttctgccaaa ttaagatgaa gtaaaagcta agtcgaccta aaaaagcacc cttctagtcg
     3001 attaatctaa aagggtgcca tatactttaa ttttaataca tgattgattc taaaaaagtg
     3061 aattattcca cagtaactga tttagcaagg ttacgtggtt tatcaacatc taaatctctg
     3121 tgtaatgctg catagtatga aattaattgt aatgcaacca ctgatactaa tggcgttaac
     3181 aattcatgta catgaggaat gacataagtg tcgccttctt tttcaagacc ctccatagaa
     3241 ataatacatg gatgtgcacc acgtgctact acctctttaa cgttaccacg aattgataaa
     3301 ttaactttct cttgtgttgc taaacctaca actggtgtac cttcttcgat taaggcaatt
     3361 gtaccatgtt taagttctcc accagcaaaa ccttctgctt gaatgtaaga aatttcttta
     3421 agttttaacg caccttctaa acttacgtta tagtcaatag tacgtccgat aaagaatgca
     3481 ttgcgtgttg tttctaagaa atctgtagca atttgttcca taattggtgc atcgtcaaca
     3541 attgcttcta ttgctgttgt tacttttgct aattctctca ataaatcaat atctgcttca
     3601 cgaccatgct cttttgcaac gatttgagac aagattgata atactgcaat ttgtgcagta
     3661 tatgcttttg tagatgcaac tgcgatttca ggacccgcgt gtaataacaa tgtgtggtct
     3721 gcttcacgtg ataaagttga acctgcaaca ttagtgattg ttaatgattt atgacctaat
     3781 ttattagttt caactaatac ggcgcggcta tctgcagttt cacctgattg agaaatataa
     3841 acgaacaatg gtttttcaga taataatggc atgttgtaga caaactctga tgcaacgtgt
     3901 acttcagttg gtacgccagc ccatttttct aaaaattctt tacctactaa acctgcatgg
     3961 tagcttgtac ctgctgcaat aacgtaaatg cggtctgctt ctttaacatc attgatgatg
     4021 tcttgatcaa ttttcaagtt accttctgca tcttgatatt cttgaataat acgacgcatt
     4081 actgctggtt gttcatgaat ttcttttaac atgtagtgtg cataaacacc tttttcagca
     4141 tctgatgcat caatttcagc aatatatgaa tcacgttcta caacgtttcc atctgcatct
     4201 ttaataataa cttcatcttt tttaacaata acgatttcat ggtcatggat ttctttatat
     4261 tcgcttgtca cttgtaacat tgcaagtgcg tctgatgcga taacattgaa accttcacca
     4321 acacctaata ataatggtga tttattttta gcaacataga ttgtgtcttt gtcttcagca
     4381 tctaataaac ctaatgcata tgaaccatgt aataatgaca caacttttgt aaatgcttct
     4441 tcagttgaaa gtccttgatt tgaaaagtat tcaactaatt gaacgataac ttctgtatct
     4501 gtttctgaaa tgaatgatac accttgtaag tattcacctt ttaactcttc atagttttca
     4561 ataacaccgt tatgaactag agtaaaacgg ccatttgatg attgatgtgg atgagagttt
     4621 tcatgattcg gtacaccgtg tgttgcccaa cgtgtgtgac cgattccaac aggtccatca
     4681 aaatcgctac tatcagcaac tttacgtaat tctgcaatac gacctttttc tttaaataca
     4741 gttgtattat catcatttac tactgcgata cctgcagagt cataacctct gtattctaat
     4801 ttttctaaac cttttaataa taattctttg gcattatcat agccaatata accaacaatt
     4861 ccacacataa cgacattttc ctccatattg gaatagtacg cgtaaattat gatttattgc
     4921 cgataattta gattgacaat ctgctttcat aatataaata ggaacatgct atcatcgcat
     4981 tcatccataa caaattaagc atagttattt ttacaactat acaaattgct cacactgtac
     5041 tttccatatt aatatttttt atattcaatt tctggcgatc ttattaactt tgtccattaa
     5101 gtcaccctaa tgttttactt aataagctaa cgaatgagcc acatccggga tagcatccgc
     5161 cgatctattc gatcactatc ctcctcgtct acaaatacat atattgcact ctataaaggc
     5221 cactcatata ttaaccttta atcttcaaat acaaatattt atttgcacag gcgctttaac
     5281 tgtactgccg aactttcccc ctttccatta atcattattg tacaacggtg ttgttttgtt
     5341 ttgcaaatat tttcacaata aaattttaaa aatcctaaaa caattttttt gttttacttt
     5401 ttcaaaatat ctatactgtc acattgatga cactttattt aattttgtca catttatttt
     5461 gacaaagttg atttttgttt atattgag
//
LOCUS       DANRDI010000166         5475 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.167, whole genome shotgun sequence.
ACCESSION   DANRDI010000166 DANRDI010000000
VERSION     DANRDI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.167"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..103)
                     /locus_tag="PZ377_002409"
     CDS             complement(<1..103)
                     /locus_tag="PZ377_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 domain-containing protein"
                     /protein_id="HDM6359265.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAI"
     gene            374..1897
                     /locus_tag="PZ377_002410"
     CDS             374..1897
                     /locus_tag="PZ377_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359266.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1965..3131
                     /locus_tag="PZ377_002411"
     CDS             1965..3131
                     /locus_tag="PZ377_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6359267.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            3128..4048
                     /locus_tag="PZ377_002412"
     CDS             3128..4048
                     /locus_tag="PZ377_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6359268.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            4062..4721
                     /locus_tag="PZ377_002413"
     CDS             4062..4721
                     /locus_tag="PZ377_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359269.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4773..>5475)
                     /gene="lytM"
                     /locus_tag="PZ377_002414"
     CDS             complement(4773..>5475)
                     /gene="lytM"
                     /locus_tag="PZ377_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDM6359270.1"
                     /translation="AQGHTHYFYNCYPKNANANGSGQTYVNPATAGDNNDYTASQSQQ
                     HINQYGYQSNVGPDASYYSHSNNNQAYNSHDGNGKVNYPNGTSNQNGGSASKATASGH
                     AKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMPENSPVYSLTDGTVVQAGWSNYGGGN
                     QVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGDQIAYSGSTGNSTAPHVHFQRMSGGI
                     GNQYAVDPTSYLQSR"
BASE COUNT         1981 a          871 c          850 g         1773 t
ORIGIN      
        1 taatcgcgtc aaaaaataat gaaactgcaa ctacaaatac tcccaataca gtcaagtatg
       61 gtctttttat attctttaca aaatcatcga ctttcaattc cataaattca taatctcccc
      121 aatcttactt caatattttt ataagttaaa taaatcttct tcgccaaaaa aatgcatatt
      181 attaagttaa ccaacatttt tcaagaatca ttatgtaatc aaactatttt ttgtagttta
      241 ttagatttcc aataacttat taaacattat tataactaaa attctattaa tttaaacaaa
      301 atttaaaata aaccatttaa taaaatgtat tgttctgata tgattaactc ataaataatt
      361 gggaggaagc aaaatgtcta aaaagttaaa aattataatt cctattatta ttgtcttact
      421 attaataggt ggaatcgcat ggggagttta tgcatttttt gctaatacac cgaaaaatac
      481 atacctaaaa agtgaacaac aaacagcaaa aatgtataaa gattatttta atgaccgttt
      541 tgaaaatgaa gtgaagttcc aagaaaagat gaaagataat tcgtttttat cttcattaga
      601 attaagcgca gatgcatcag atgaaattgt taaagggctt ggcattccta aatctgttgt
      661 taatgcatcg aaaattaaaa tgtcatatgg acatgatcct aaaaaagaga aatcaatgat
      721 taatcttgaa ccaacaatcg cagactctgc attagggaaa ttccagttag ctgcagataa
      781 agataagcat tatttcgaat caccattatt taaagggaaa tatagtgtta ataattctga
      841 tttattatca acttattcaa aacttacagg tgaagatgaa gaaacagcaa aagaaaatgg
      901 tattacaaac caacaactaa atttaaatac tctttttaat aatgctcaag cacaacaaag
      961 tgactacagc aaaattgccg aaaaatattc cgaacttatt gtcgacaaat tagatgacga
     1021 taattttgat aaaggtaaaa aagaagaaat taaggttaat ggtgaaaagt acaaagttag
     1081 acctgtcacg ttaacactta gcagagctga cactaaaaaa attacattag ctgtattaga
     1141 agaagctaaa aaggataaag accttaaaaa attaatggaa gaacaaggtg ctacaaaaga
     1201 ctttgaaaaa gacatcaaaa aagcaattga cgatgtcaaa gaaactaaaa aggatgaatt
     1261 tgctaaaatt caatctaaaa tttataccga aaaacatacg attgtaaaac gagaaattac
     1321 tattacagac aaagaaaata ataaaactaa aatcaaaggt actaatactt tagaagacga
     1381 taagttaaaa ctagattacg cacttgattt cgatcaagat aaatacacgt atgctgaagc
     1441 gaaatataca attaaaggcg tatcttctaa agaaaaagat aataaatata atgataaata
     1501 cgaatttggt aaaaagacag aatatgatga atcaaaaatc aaattagata accaagaaaa
     1561 agtagatggc acaaaacgtc aagataaagg taaaatcact gtcgcattag ataaatatag
     1621 cgacgaaaat gaattcactt ttgaaaataa tatagattct gacgtaaaaa ataacactca
     1681 gaaatctacg ttaaatatcg gcatcaaata tgctgaagaa ccaattaatt tcattttaaa
     1741 atctagcaca aaattgaaag cagatattga ttttgatgat agtggtgcaa aagatttcaa
     1801 tagtctatct tcaaaagacc gtgaaaaact tgaaaaagaa atcgaaaaaa atggcggcaa
     1861 aatgtttgaa tcaattttaa aaaaggcatc taaataacta aaatataaaa ataagattgg
     1921 aaggtcatct tgcctttcaa tcttatcatt ggaggagtgg ttaaatgaaa acaatacatt
     1981 tgtttcgcat ctaccactct tttttattga aaaagtggta tttgattatc tatttactat
     2041 ttatattagc ggcactcctt attacattaa cgacaatcca acatgtaaca gaagatgaca
     2101 atcattttaa tataggtgtc gtagataaag atcaatcaag tgaaacgaaa ttaatcttaa
     2161 actctattgg taaagggagt aacctaggaa aaaacgtgag cattaaagca tatgatgata
     2221 agcaagcaca tactttgtta aaaaaacata aacttcaagg ctattttgtt tttgataaag
     2281 gtatgaccaa ggcattttat aaacaaggcg aactaccaat ttcagtatat acatatgatc
     2341 aacaatccat gaaaagtgtc gtgctatctc agctaacaga ttctgtttac caacgtctta
     2401 tgcgatcaat gggtggcatc ttagcttttc aagacttagc accgaaagca tcacattctg
     2461 acagtatcaa tgttatgact gatttgctga ttacaggatt aaaccgttca ggtgcattta
     2521 acttagaacc gattcattta tacgatacgg gcagttatta tgcaattaca ggatttttaa
     2581 caacggtatt catctttgca ctatctttat ttacagtttt gaaaatgaat caagatactg
     2641 tattgaaagc gcgattgaaa atgtttcatt tttctaaaga gcgtttatta atcattcgta
     2701 cgttgattac atggttttat actatgttat ggagtatcgt tggtgtagtt tggattgtgt
     2761 tcagtattcc gaatatcttt gaattatata attggccaac gttagccatt catttaagct
     2821 attatgttac ctttttaata ctgtggttat tactcatcga acttttaaca acaggcttgt
     2881 tgaatagtat tagtaaagtc atattagcta ttgtcatcct ggtgttatct ggtttaacaa
     2941 tacctacgat ttttttacaa catatagcaa atggtgtttt caatattcaa ccgttcgcag
     3001 ttgtaacaaa tcaattgtta gaaattattt taaacaacta cattttagaa ctgcacccta
     3061 gtttctatct tagcttcatc gcacttttga taattaattt agctgtattg gtttggaggt
     3121 atcgtcaatg aagccttata tacaacttgt tgtgttcaag caatggttac aatacatctt
     3181 gctcgtaaca accattgtca tcgcactcgt acttattggt atcggttacc gtgtagcaca
     3241 tgacaacttc aaaataccga ttaccattca agatttagac caaaccactg catcaaaatc
     3301 attcgtcaat aaaattaaac aatctgacta tgtaactatt aaaaaagtcg atgaagatga
     3361 aagctatatt gaagatgatg ttactaaaaa ggaagctatt ttaagtatgc aaattcctaa
     3421 aggtttctct caaaaattaa aagagaaccg tttaaaagaa acgatacagt tatatggtag
     3481 agatgacttt ataggtggta ttgctgtaga aattgttagt agttcattat atgagcagca
     3541 aattcctaac attatttatg aacaccttga ggatatgaaa cagcatcaat ccatcgatgc
     3601 tatcaacaag tcctatcata aacatacacc tgaatctaaa atcaaatttg tgtcgcttac
     3661 taaacaagca caacactcta tttcaattag cttaatcttt gcggtgattt tatttgttag
     3721 cgctgttcaa gtagtacttc attatcgttt aaaccaacaa gcagcattgc aacgattatc
     3781 acaatatcat ttaagccgtt tcaaactata tagtacttat gtaatgacac atacgatttt
     3841 gttattgttg gtactattgg cagttagtct atatttgtct caaccactca gcttaatatt
     3901 ttacttaaaa tcactgttac ttatattgat ttatgagata ggtatcgtct ttatcttatt
     3961 ccatattcaa acaataagtc atcgattatt catgacattt atatatgcac ttgctatggg
     4021 tatcgtatac ttgattattt tcatgtaaag gagcgtaact gatgatagaa attaataacc
     4081 tttcaaagcg ttaccgtaac aaacagattt tcaatcattt aactatgtcc tttgatagta
     4141 atcgtttaac cgtattactt ggtgataatg gtgctggaaa atcaacatta cttcgtatga
     4201 ttgctggtat tgaaaaagct aatgatggaa ctatcaacta tttcggcgaa aaatggaatc
     4261 aaagacaaat acaaaatcac atcggttatg tgccacaaga cattgcgtta tttgaacaca
     4321 tgacagtggc tgaaaacatt aaatttttta aatcactttg taaaaatcca attaacgata
     4381 caactatcaa cgaatattta cagcaattaa actttgatga tacgtctgcc aaagtatcta
     4441 cattgtccgg tgggaataaa cgtaaaatta atatattagt aggtttacta ggtcaacctc
     4501 gaattctcat tttagatgaa ccgacagttg gtattgattt aaaatctaga catgacatcc
     4561 accaactact taacatcatg aaatctaaat gtttaattat attaactacc catcatttag
     4621 atgaagttga agcacttgca gatgatatca agttaattgg ccaagatcct ttttatcaac
     4681 atgttttaga ggacaaacaa tggacttata cctattatta aaacgaaaaa atcccaagct
     4741 gcgtatgata tcgcaacttg ggattttctg tattatctac tttgcaagta tgacgttggg
     4801 tctactgcat attgattacc gatgccacca gacatacgtt ggaagtgtac gtgaggcgct
     4861 gttgaattac ccgtactacc tgaatatgca atttggtcac cagctttgac tttatcacca
     4921 gctgaaacag ttaaacgatt attatgcata taccattggt agttattact gttcgcttct
     4981 ttaatcgtta cttgattgcc gccaccatag ttactccaac ctgcttgtac tactgtacca
     5041 tcagttaatg agtaaactgg tgaattttca ggcattgcat agtcgacacc gtaatgcgca
     5101 ccaccaccgt gatattgtcc atatggttgt agttgtttac gacttgttaa ccagcttgcg
     5161 tctttcgcat gaccactagc tgtcgcttta cttgctgatc caccattttg attagatgtg
     5221 ccattaggat aattgacctt tccattacca tcatggctgt tatacgcttg gttgttgtta
     5281 ctatgtgaat aatagctcgc gtctggacct acatttgatt gataaccata ttgattaata
     5341 tgctgttggc tttgactcgc tgtgtagtca ttgttatctc ctgctgttgc tggattcaca
     5401 tatgtttggc cgcttccatt ggcatttgca ttttttggat aacagttata aaaataatgc
     5461 gtatgtcctt gagca
//
LOCUS       DANRDI010000167         5467 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.168, whole genome shotgun sequence.
ACCESSION   DANRDI010000167 DANRDI010000000
VERSION     DANRDI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.168"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..471
                     /locus_tag="PZ377_002415"
     CDS             <1..471
                     /locus_tag="PZ377_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDM6359271.1"
                     /translation="IPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQ
                     LSDLVLLLSEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSAS
                     DYAIRLLEDKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            476..1678
                     /locus_tag="PZ377_002416"
     CDS             476..1678
                     /locus_tag="PZ377_002416"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDM6359272.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            1665..2636
                     /locus_tag="PZ377_002417"
     CDS             1665..2636
                     /locus_tag="PZ377_002417"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDM6359273.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            2660..5353
                     /locus_tag="PZ377_002418"
     CDS             2660..5353
                     /locus_tag="PZ377_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HDM6359274.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
BASE COUNT         2049 a          701 c          988 g         1729 t
ORIGIN      
        1 atacctagta aattaatttt acttggagat ggtcctgaat tagtgccaat gcgtcaattg
       61 acgaaagagt taaacgtaga agaggatgtt ttatttttag ggaaacaaga ttgcgtaagt
      121 gaattttacc aattatctga cttagtatta ttattaagtg aaaaagaaag ctttggacta
      181 actttacttg aggctatgaa aacaggcgtc gttccaattg gttcaaatgc cggtggaatt
      241 aaagaggtta ttaaacatgg tgaaactgga tttgtcgtag atgtgggtga ttgtgattca
      301 gcgagtgact atgctatccg attacttgaa gataaggtgt tatacaacaa acttcagaaa
      361 aacatgctag cagatattgc tgaacgtttt ggatcagaac ttattacaga tcaatatgag
      421 tattattatc aaaaaatgct aaatgagcat aacaaaagta aaggcgagta ataatatgga
      481 taaatcatta tttgaacagg caaggcctat attagaacaa attcaagaca atggttttga
      541 agcatattat gtaggtggct ctgtaagaga ttatgtcatg ggaagaaata ttcatgatat
      601 agatatcaca acaagtgcaa cgccggatga aatagaatct atctttagtc atacgatacc
      661 tgtaggtaaa gaacatggca cgataaatgt agtttttaat gatgaaaatt atgaagtgac
      721 aacattccgg gctgaagaag attatgtcga tcaccgtaga ccaagtggtg ttacatttgt
      781 tcgtgattta tacgaagatt tgcaacgacg agatttcacg atgaatgcga tagcaatgga
      841 tacagcatac aaattgtatg attattttga tggtcaacaa gatattaata atcgaataat
      901 aagaactgta ggtatagctg aggaacgatt ccaagaagat gctttacgta tgattcgatg
      961 tttaaggttc cagtcacaat tatcatttga tattgcaacg gaaacattcg aagcgatgcg
     1021 tatacaaatg gcagatatta aatttttatc aattgagcgt atagtgattg aactaactaa
     1081 attaatgcga ggtattaatg ttgaaaagag ttttaatcat ttaaaatcgc tgaaagcatt
     1141 taattatatg ccgtatttcg aacatcttga tatgaatcaa attaatgtaa ctgaagcaat
     1201 tgatttagaa ttgttgattg ctatagtatc agttaaattt gatattaatt actcattgaa
     1261 gcctttaaag ctaagtaacc gacaagtaaa agatatcaat caatatattc aaattatgaa
     1321 tgcattacca agtattatta caaaagaaca attaaaaatg tttgtttatg attatgatac
     1381 gcatctcatt aaaaatgtaa tggttgcagc agacgtgtta aaggcaaatg atattcaagg
     1441 acatgaacca ttaatcgtta accttcaaac gattgatgaa acattacatc gtttacctat
     1501 gcataataga aaagacatga tggttaatgg cggtgtactt atggcacatt taaatgccaa
     1561 aagtggtccg tggttaaaag atgtgctaag acaaattgag atagcgattg taacaggtaa
     1621 agtaagcaac gaagaaactg aaattttgaa atgggtggat aatcatgtca aaatatagtc
     1681 aagatgtact tcaattactc tataaaaata aaccgaatta tatatctgga caaagcattg
     1741 cggaatcact taatatttca cgcactgcag taaaaaaagt gattgaccaa ttaaagttag
     1801 agggatgtaa aatagattca gtaaatcata aagggcattt attacaacag ctcccagata
     1861 tttggtatca aggtataata gaccaatata caaaaagttc tgctttgttt gattttagtg
     1921 aagtatacga ttcaatagat tctacacaac ttgctgcgaa aaagtcactt gttggaaatc
     1981 aatcttcatt ttttatcttg agtgatgaac aaacgaaagg tcgtgggcga tttaatagac
     2041 attggagttc ttcaaaaggg caaggacttt ggatgtctgt cgtgttaaga cctaacgttg
     2101 cattctcaat gatatctaaa tttaatttat ttattgcatt agggataaga gatgcgattc
     2161 aacattttag tcaagatgaa gtcaaagtga aatggccgaa tgatatatat attgataatg
     2221 gtaaagtgtg tggtttctta actgaaatgg ttgctaataa tgatggtata gaagcaataa
     2281 tatgtggtat aggtattaat ttgacgcaac aactagaaaa ctttgatgaa agtattagac
     2341 atagagcaac aagtatacaa ttacatgata aaaataaatt agatagatat caatttttag
     2401 agatattact tcaagaaatt gaaaaaagat ataatcaatt tttaacgtta cctttttctg
     2461 aaattcgtga agaatatact gcagcttcta atatttggaa tagaacgttg ctatttacag
     2521 aaaatgataa acagtttaaa ggacaagcaa ttgatttaga ttacgatggc tatctaattg
     2581 ttagagatga agcgggtgaa tcacaccgtt taattagtgc agatatagat ttttaacact
     2641 aaagcaagga gagatagcta tgggtatggc aacctatgcc gttgtggatt tggaaacaac
     2701 aggcaaccaa ttagattttg acgatatcat tcaaattggt ataacgtttg tgcgcaataa
     2761 ccaaattatt gatacatatc attctatgat tagaacaaat ttagagattc cgccgttcat
     2821 tcaagcgtta acatccattg aagaaaatat gctacaacaa gcgccatatt ttaatcaagt
     2881 tgcacaagaa atctacgata agataaaaga ttgcattttt gtagctcata atgtagattt
     2941 tgatttgaat tttattaaaa aggcatttaa agattgtaat attcaatatc gtcccaaaaa
     3001 agttatagat acactagaaa tctttaaaat agcttttcct acagataaaa gctatcaatt
     3061 aagtgaatta gcagaggcac atggtattac cttagcaaat gcccatcgag ctgacgaaga
     3121 tgccgctact actgctaaat tgatgatatt agcttttgaa aaatttgaaa agttaccgtt
     3181 agatactttg aagcaattat attatttaag taaacagttg aaatacgatt tgtatgatat
     3241 tttctttgag atggttaggc aatatgatgc aaagccattg gataaatcgt acgaaaagtt
     3301 cgaacagatt atctatcgta agcaagttga ttttaaaaag ccgacaacaa attataatgg
     3361 cagcttgaag tctttatata gcaaagctgt agatcaactt ggcttaacat atcgaccgca
     3421 acaattatat ttagcggaaa ctatattaga tcagctcatg catagtgaaa aagcaatgat
     3481 tgaagcatca ctaggcagtg gtaaatcatt agcatattta cttgcggcct tgatgtataa
     3541 tattgaaact gggaagcatg tcatgatttc taccaatact aaattactgc aaagtcaatt
     3601 gttagaaaaa gatattccag caatgaatga agcgttgaat tttaaaatta atgcactatt
     3661 gattaaaagt aaaagtgatt acatttcatt ggggcttatt agtcaaattt taaaagatga
     3721 cacaagtaat tacgaagtga atatcttgaa aatgcaattg ctgatttgga ttacagagac
     3781 gccgtccgga gatatacagg aattaaattt aaaaggtggt caaaagatgt attttgacca
     3841 gaaaattgaa acatatgtac cggcacgtca cgatgttcat tattataatt ttattaagcg
     3901 aaatgcacag aacattcaaa ttggtattac aaatcatgca catttaattc attctgatgt
     3961 tgaaaattca atatatcaat tgtttgatga ttgtatagtg gatgaagcac atcgcttacc
     4021 agactatgcc ttaaatcaag tgacaaatga gttgagttat gctgatatta aatatcagct
     4081 tggtttgatt ggtaaaaacg aaaatgaaaa attgttaaag gcaattgatc aactggaaaa
     4141 gcagcgaatt ttggaaaaat tagatattgc accaattgat atttttggct tgaaggctag
     4201 tatgaatgag attcatgagt taaatgagca attgttctca acgatattta cgattatcaa
     4261 tgattctgat gtttatgatg atgatattca tcgcttccat aatgtattta cttttgaaac
     4321 aaaagacatt ttgaaggatt tacatgcgat tatcgataag ttaaataaaa cgctagaaat
     4381 attcaatggc attagtcata aaacagttaa atcattacgg aaacagttgt tgtatttaaa
     4441 agataaattt aaaaatatcg aacaaagttt aaaagctgga catactagtt tcatttcgat
     4501 taaaaattta tctcaaaaat caacaatacg tttatatgtg aaagattacg ctgtaaaaga
     4561 tgtattaacg aaacaagtat tggaaaaatt taaatcgctc atttttatat ctggtacttt
     4621 aaaatttaat cattcgtttg aagcttttaa acagttattc aacaaagatg ttcactttaa
     4681 tacatttgaa gttaacacgt cgttacaaag tgcaaaaaat acaagtgtat ttataccgag
     4741 tgatgtagca tcttatcaat ataaaaatat agatgagtat gtagcatcga ttgtaagcta
     4801 tattattgaa tatacgacta taacgtcatc aaaatgttta gtcttattta cgagttataa
     4861 aatgatgcat atggtacaag atatgctaaa tgaattgcca gaatttgaag attatgttgt
     4921 attaacacaa cagcagaacc aaaattataa aatagtacaa cagtttaata attttgataa
     4981 ggccatactg cttggtacgt caacattttt tgaaggtttt gattttcaag caaatggtat
     5041 taaatgtgtg atgatagcga agttaccgtt tatgaacaag cataatgcaa aatattggct
     5101 tatggattct gaattcactt caactttcaa ggaatatgta ttacctgatg cagttacacg
     5161 ttttagacaa ggacttggaa gattaattag aaatgaaaat gatcgcggca taatagtttc
     5221 atttgatgat cgactcataa acagtaatta taaaaacttt tttgaacaaa cacttgaaaa
     5281 ttatcgtcag aaaaaaggcg acattcaaca gtttggaaaa ttattaagac aaattcaaaa
     5341 aaagaaaaag tgaaatacaa agtaaaattc ttgtgacatg acatgtgcta tgttctgtta
     5401 caagaatttt tgtttggtcg tcaaaatttg agttttataa ttaaacttga atgttagcac
     5461 aaaaaac
//
LOCUS       DANRDI010000168         5439 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.169, whole genome shotgun sequence.
ACCESSION   DANRDI010000168 DANRDI010000000
VERSION     DANRDI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.169"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(50..2077)
                     /gene="kdpB"
                     /locus_tag="PZ377_002419"
     CDS             complement(50..2077)
                     /gene="kdpB"
                     /locus_tag="PZ377_002419"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDM6359275.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(2096..3772)
                     /gene="kdpA"
                     /locus_tag="PZ377_002420"
     CDS             complement(2096..3772)
                     /gene="kdpA"
                     /locus_tag="PZ377_002420"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDM6359276.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(3796..3906)
                     /gene="kdpF"
                     /locus_tag="PZ377_002421"
     CDS             complement(3796..3906)
                     /gene="kdpF"
                     /locus_tag="PZ377_002421"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDM6359277.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            4044..>5439
                     /locus_tag="PZ377_002422"
     CDS             4044..>5439
                     /locus_tag="PZ377_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDM6359278.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFD"
BASE COUNT         1871 a         1012 c          802 g         1754 t
ORIGIN      
        1 taaacaaata ctatttctta tcgtattcat ggtacaggct ccttcctctt tacacaaaaa
       61 attgtacaat catatctatt aatttaatgc ctaaaaacgg gacgattaat ccacctaatc
      121 cataaatcaa catattattt ataaagattc tatcaatgct gtaacccttt acttttacac
      181 ctttcatggc aattggaatt aaggcaacaa tgattaatgc attgaatatc aaagctgata
      241 taattgcaga tttaggtgac gataaatgca taatatttag tgaagtcatt tctggaattg
      301 ttgacatcat tagtgcaggt aaaattgcaa agtattttgc tacgtcatta gccaaactaa
      361 atgtcgttaa tgcacctctc gtcattaata attgtttgcc tatttttaca acctctatta
      421 actttgtagg attcgaatct aaatcaatta gattagctgc ctctttagca ctaattgtcc
      481 ctgagttcat agctaatcct atattcgctt gtgctagcgc aggtgcatca tttgtaccat
      541 ctcctgtcat cgcaacaata tggcctttcg cttgttcatc tttgatgact ttaattttat
      601 cttcgggttt acactctgca acaaatctat caaccccggc ttcttttgca attgtagctg
      661 ctgttaaagc attatcacct gtacacataa ctgtttcaat ccccattttt ctcaattcag
      721 taaatcgttc tacaagacca tctttaatca catcttttaa ataaatcacg ccaagcatga
      781 cattgttttc aatgactatt aatggtgtgc cacctttact cgatacatcc atacagagag
      841 actcaatatt aagaggaata ttgccttgtt gttgtttgac aagatttatc atactattag
      901 gtgcaccttt gaataccgat atttcatttg taatgattcc gctcattcta gtttcagctg
      961 taaaaggctt atatgtgcca tcaatgtctt taggcagctc atttatatac atctgcttcg
     1021 ctaatcgtac aatacttttt ccttctggcg tatcatcgta gattgatgac atataagcag
     1081 cgactatcaa tttttcaagc atttgttgat tcactggtaa aaattcacta gcgattcgat
     1141 tgccataagt gattgtgcct gtcttgtcta aaatcattac atcgacatct ccacatactt
     1201 ctacagcacg cccacttttc gctaatacat tgaattgagt aacacgatcc atgcctgcaa
     1261 taccaatcgc cgataacaaa ccaccgattg tcgttggtat taaacatact gttaacgcaa
     1321 tgagcatcgc aataggtaaa attaaatgca ggtaagatgc tattggatat aacgttacaa
     1381 taacgactaa aaatataatt gttaacgttg ttaataatgt aaaaagtgca atttcatttg
     1441 gtgttttatt tctttccgcc ccttcaacta aggcaatcat tttatctaaa aaagatgtac
     1501 ccgcttcact ctcaacacgt atttctaacc aatcagatgt tacaagtgta ccgccaatga
     1561 ctccatcaaa atcgccacct gattctttta tcacaggtgc agactcacca gtaattgcag
     1621 attcatcaac ggttgctaat ccatttatta caacgccatc agcagggatt gtttctccat
     1681 tttctacccg aatattttgt ccggctttta actctgtggc gttcactatc cgatacgcac
     1741 cattttcttc tatcaatcga gcagttaaat ttgattgtgc ttgtcttaaa ctatcagctt
     1801 gcgcttttcc acgaccttca gcaaaggctt ctgaaaaatt agcaaacaat atagttatta
     1861 ataatatgat aaaaattgta atcaaataac ctcgcgatag atagctagtt ccaaatatgt
     1921 caggaaaaca tattaatatc aacgttaaaa tcattccaac ctcaacgaca aacattatcg
     1981 gattttttat taattgttta agattcagct tataaaaact cattttcaaa gcttctatca
     2041 ccattgtttg attgaaatat ttatttacat gatgcatttt ttccatccct ttactttatt
     2101 ttaaagttaa aaattcacca ataggaccaa gtaatagtac tggaataaat gtcaaaccac
     2161 ttagtaaaac gataaatacg attagtgata cgccaaaata aggtttatca atcgctattg
     2221 tatatttatc ttgatggtat gattttttat tcactaaact tgatgcaatc attaattgca
     2281 aaataattgg tatataacga gaaagcaaca taatgattcc tgtagagata ttccagaatg
     2341 ttgtatcatc tttcagtcct tcaaaccctg atccattgtt cgcagcagct gatgtcattt
     2401 catacataac ttgtgaaata ccatgaaaag acggattcgt tatactttca cttgctccag
     2461 gaatcataaa agcaagtgct gaaaatacta aaattaaaat tgggtgtatg agaaagacta
     2521 agacaataca tttcatttca cgggcgccaa ttggcatatt taaatattct ggtgttttac
     2581 caaccatcaa actgcatata aacaccgtca gtaagacaaa tatcaataaa ttcatgagtc
     2641 ctacgccttc gccaccaaat acaacattta gcatcattaa taccattggt cctaatccac
     2701 ctataggcgt taagctatca tgcatgttat taacagaacc cgttgtaaat gccgtcgtaa
     2761 taactgtaaa tagtgctgac aaacctgctc caaaccgtac ctctttacct tccatattcg
     2821 gtccataaat gcctaaattc gctagtattg gattaccacg atactcactc cacatagtta
     2881 atgtaagaat tgctataaaa atgaaaaaca ttgcgacaaa taatatcaac gcatgacgat
     2941 gtactcgttt accatgtcta cttaacatgc gaccaaataa gaacaacatt gacataggaa
     3001 gtaacatcat actgcccatt tctataaaat tgctccaaat atttggattt tcaaaaggtg
     3061 tcgcagaatt tcctgctaaa aatcctccac cattcgtacc aagatgtttt attgattcaa
     3121 gtgatgcaat aggtccaaat gcaatatgtt gaatatgtcc gcttaaagtc cgaatcatta
     3181 aattagcatg caacgtttgt ggtacacctt gagtcatcaa taaaatacta attaaacatg
     3241 ataatggtaa aagtactcgg acaataaacc gaacaatatc ttgataaaaa ttaccaatga
     3301 tattagttaa tccagttaaa cgtctcaaca tcgctataca aacggcgtaa cctgatgcac
     3361 tagatgtaaa cattaaatat gtcattacaa tcatttgcgt taaatatgtc acatctgatt
     3421 caccgttata gtgttgtaaa ttactatttg ttaaaaaaga tattgctgta ttaaacgcta
     3481 aatctatcga ttggtttaaa ttatgatttg gatttaaaaa aagccattgc tgaactatta
     3541 gcaatacaaa tgttataaac cccataaatc cattaaatgc cagaaaatgt ttgacatatg
     3601 ttttagctga catgtgttct aaatctgtgc cgataatttt aaaacacata ttttcaaatc
     3661 tagtaaatat taaatctact cttgacgatt gcaccaatgc tacgcgatat agatatccac
     3721 taaaaacata cgtaatcata accatcattg ttagaaacaa aattatttcc atgataaccc
     3781 tcacttaata tatttctaaa atttttcact acgaattaag gcataaaata aatacaaaac
     3841 taatgcaata actaccagta ataaaacgat gagcattgcc ataacctcct tacaacacaa
     3901 caacatcgta acaacttgtt tatgagagaa atattaattt tcaaacttag ttattaagaa
     3961 atcattaaga tgtgtatgca gaaataaatt ttatagcatt taattgtgaa gaatattatg
     4021 atattgctat cgaggtgaag gttatgtcaa acactgaatc gctaaacata ggaaaaaagc
     4081 gtggatcttt aacaatttac atcggttaca gtccaggtgt tggtaaaaca tttgagatgc
     4141 tttcaaatgc cattgaacta tttcaaagta acgttgatat taaaatagga tatattgaac
     4201 cgcatcagcg tgatgaaaca aatgcattag ctgaacaatt acctaaaatc acaaccaatt
     4261 ttactaaaca tggtagtcat cattttcaat atttagatgt cgaccgcata atcgaagaat
     4321 cgccgacaat agtacttatc gatgagttag cacatacgaa tatttctaga gatcgtcatg
     4381 agaaacgata tatggatatt gaagaaattt taaatcatgg tatcgatgtt cataccactt
     4441 tgaacattca acatattgaa agtttaagta gtcaaattga actgatgacc ggtgtacatg
     4501 ttaaagaacg tgtacccgac tatttcataa tgagcgccga tgtattagaa gtcgtagata
     4561 tctcacctga acaattaatt aaacgcttaa aagctggcaa ggtatataaa aaggataggc
     4621 tagatgtagc atttagtaat ttctttacgt atgcccacct aagcgaactg cgtacattga
     4681 cgttaagaac agttgccgac ttgatgagtg ataaagaaaa agtccgacac aaccataaaa
     4741 cgtcactcaa acctcatatt gctgtggcaa ttagtgggag catttataat gaagcagtaa
     4801 ttaaagaggc attccatatt gctcaaaaag aacatgcgaa gttcactgct atttatatag
     4861 atgtattcga aaaaaacagg caatataaag atagtcaaaa gcaagtgcat caacatctca
     4921 tgcttgcaaa atcattagga gcaaaagtaa aagtagttta tagccaaacc gttgcattag
     4981 gattagacga atggtgtaaa aatcaagatg taaccaaatt aattatcgga caacatatta
     5041 gaaataagtg gcgagacttt ttcaatacac ctttaattga ccatttaatg tcctttgaac
     5101 atagctataa aatcgaaatc gttccaatca aacaaatacc tgttgaattg aaaatgaaca
     5161 aatcacccta tcgtcctaaa ggcaaacgtt tcgccataga tatgttaaaa atgattttga
     5221 ttcaaataat ttgtgtaatg atgggactgt ggatttatca acttgataag catgagtcta
     5281 gtacgattat tttaatgatt tttctcatcg gcatcatttt attatccatt tggacgcggt
     5341 ccttcatcat tggcttttta gcagcaatta ttaacgtatt tgtttttaat tattttttta
     5401 cggaacctag atatacattt gaagtatatc gctttgact
//
LOCUS       DANRDI010000169         5368 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.170, whole genome shotgun sequence.
ACCESSION   DANRDI010000169 DANRDI010000000
VERSION     DANRDI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.170"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..141
                     /locus_tag="PZ377_002423"
     CDS             <1..141
                     /locus_tag="PZ377_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDM6359279.1"
                     /translation="PFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQ
                     LQ"
     gene            251..1030
                     /locus_tag="PZ377_002424"
     CDS             251..1030
                     /locus_tag="PZ377_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDM6359280.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            1054..1671
                     /locus_tag="PZ377_002425"
     CDS             1054..1671
                     /locus_tag="PZ377_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDM6359281.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            1892..4645
                     /gene="ileS"
                     /locus_tag="PZ377_002426"
     CDS             1892..4645
                     /gene="ileS"
                     /locus_tag="PZ377_002426"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDM6359282.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            4935..>5368
                     /locus_tag="PZ377_002427"
     CDS             4935..>5368
                     /locus_tag="PZ377_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6359283.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRP"
BASE COUNT         1962 a          767 c         1019 g         1620 t
ORIGIN      
        1 cctttcttac aaccatttag aaaagtaatt ccacctattg gaattatcga catatcatca
       61 atcgctgcaa ttatcgtttt agtattattc caaaaagggt tactccaaat ctttaattgg
      121 attttaattc aattacaata aattaagcga cataacgagg caagggtaca tgatacttta
      181 gcctcgtttt tgatatgcat ttttctgaat ataagggcaa tagatggtat tttataattt
      241 ttttaaggta gtgattaaca tagatattta tcaacacttt agacaggaag aatacgaatt
      301 aattgatcag ctaacggata aatgtgatca agcggaacag cattatgcac cagtattaac
      361 gcatttttta gatccaagag ggcaatatat attggaagtg atttgtggca gttatgaaga
      421 tttaaacgta tctttttatg gtggacctaa tgctgaaaga aaaagagcaa tcatttcgcc
      481 gaactattat gaacctaaag aaagcgactt tgaattaact ttaatggaaa tagattatcc
      541 tgaaaaattc gtcactttaa aacatcaaca tattttaggg acattaatgt ctttaggtat
      601 cgaacgcgaa caagttggag atataattgt gaatgaacga attcaatttg ttttgacaag
      661 tagattggaa tcatttatta tgttagaatt acaacgtatt aaaggcgcat cagttaaact
      721 ttatactatt ccagtaacag atatgataca atctaatgag aattggaaaa atgaaagtgc
      781 aacagttagt tctttaaggt tagatgttgt tattaaagaa atgatacgta aatcacgtac
      841 gattgcgaaa caactaatcg aaaaaaaacg tgttaaagtg aatcacacta ttgttgattc
      901 agcagatttt caattacaag caaatgattt aatatccatc caaggttttg gtagagcaca
      961 cattactgac ttaggtggta aaactaaaaa agataaaacg cacattacct atagaacatt
     1021 attcaaatag taatgattta aggaggataa caaatgcctt ttacaccaaa tgaaattaag
     1081 aataaagagt tttcacgtgt aaagaatggt ttagaaccta ctgaagttgc taattttttg
     1141 gagcaactaa gcactgaaat tgaacgtctt aaagaagata aaaaacaact tgaaaaagta
     1201 atcgaagaga gagatactaa tattaagtct tatcaagacg tgcatcaatc tgtaagtgat
     1261 gctttgatac aagctcaaaa agctggtgaa gaaactaagc aagctgcaga gaaacaagct
     1321 gaagcgatta tagctaaggc agaagcgcaa gctaatcaaa tggttggtga cgcggtagaa
     1381 aaagcacgcc gtttagcatt ccagactgaa gatatgaaac gtcaatcaaa agtatttaga
     1441 tcgcgtttcc gtatgttagt tgaagcgcaa ttagacttat taaaaaacga agattgggat
     1501 tacttgttga attatgattt agacgctgaa caagtgacgc ttgaaaatat tcatcatttg
     1561 catgaaaatg atttaaagcc agatgaagtt gcagcaaatg cacaaaataa tgcatcaaat
     1621 acaccagaca ataatcaaca atccaatgat tcagaaacaa ctaagaagta agaattaaat
     1681 aaagacagac gcgtaatata catttaactt ttcacagcga attaggtaat ggtgagagcc
     1741 tagtaaaagc atgtatgtta tatcactggc tttttaatat ttaaataatg taatgagaga
     1801 actctaagtt gagttaataa gggtggtacc gcgagcaatc gtccctttta atttaactta
     1861 gagtttttta aatttttaag gagtgaaaaa aatggattac aaagaaacgt tattaatgcc
     1921 taaaacagat ttcccaatgc gaggtggttt accaaacaag gaaccgcaaa ttcaagaaaa
     1981 atgggatgca gaagatcaat accataaagc gttagaaaaa aataaaggta acgaaacatt
     2041 cattttacat gatggcccac catacgcgaa tggtaactta catatgggac atgccttgaa
     2101 caaaatttta aaagacttta ttgtacgtta taaaactatg caagggttct atgcaccata
     2161 cgtaccaggt tgggatacac atggtttacc aattgaacaa gcattaacga aaaaaggtgt
     2221 tgaccgaaag aaaatgtcaa cagctgaatt ccgtgagaaa tgtaaagaat ttgctttaga
     2281 acaaattgaa ttacagaaaa aagattttag acgtttaggt gttcgtggtg actttaatga
     2341 tccatatatt acattaaaac ctgaatacga agctgcacaa attcgtattt ttggagaaat
     2401 ggcagataaa ggtttaattt ataaaggtaa aaagccagtt tattggtctc cttcaagtga
     2461 gtcttcatta gcagaagcag aaattgaata tcacgataaa cgttcagcat caatttacgt
     2521 tgcatttgac gttaaagatg acaaaggtgt cgttgatgca gatgctaaat ttattatctg
     2581 gacaacaacg ccatggacaa ttccatcaaa tgttgcgatt accgttcatc ctgaattaaa
     2641 atatggtcaa tacaatgtaa atggcgaaaa atatattatt gcagaagcct tgtctgacgc
     2701 tgtagcagaa gcactggatt gggataaagc atcaatcaaa ttagaaaaag aatacacagg
     2761 taaagaatta gagtatgttg tagcacaaca tccattctta gacagagaat cgttagtgat
     2821 taatggtgat catgttacta cagatgctgg tacaggttgt gtacatacag caccaggtca
     2881 cggggaagat gactatattg ttggtcaaaa atatgaattg ccagtaatta gtccaatcga
     2941 tgataaaggt gtatttactg aagaaggcgg ccaatttgaa gggatgttct atgataaagc
     3001 taataaagcc gttactgatt tattaacaga aaaaggtgca ctattaaaat tagactttat
     3061 tacacatagc tatccacacg actggagaac aaaaaaacct gtaatcttcc gtgctacacc
     3121 acaatggttt gcctcaatca gtaaagtaag acaagatatt ttagatgcaa tcgaaaatac
     3181 aaacttcaaa gtaaattggg gtaaaacacg tatttacaat atggttcgtg accgtggcga
     3241 atgggttatt tctcgtcaac gtgtgtgggg tgtaccgtta ccagtatttt atgctgaaaa
     3301 tggcgaaatt atcatgacga aagaaacagt gaatcatgtt gctgatttat ttgcagaaca
     3361 cggttcaaat atttggtttg aaagagaagc gaaagactta ctaccagaag gatttacaca
     3421 tccaggcagc cctaacggta catttactaa agaaacagac attatggacg tttggtttga
     3481 ttctggttca tcacaccgtg gcgtgttgga aacaagaccg gaattaagtt tcccagcgga
     3541 tatgtattta gaaggtagtg accaatatcg tggttggttc aactcttcta tcacaacttc
     3601 agttgctaca agaggagtat caccttataa attcttactt tctcatggtt ttgttatgga
     3661 cggtgaaggt aagaaaatga gtaaatcttt aggtaatgtg attgtacctg accaagtggt
     3721 taaacaaaaa ggtgctgata ttgcgagact ttgggtaagt agtacggact atttagctga
     3781 tgttagaatt tctgatgaaa ttttaaaaca aacatctgat gtttatcgta aaatcagaaa
     3841 tacattaaga tttatgttag gtaacattaa cgatttcaat cctgacacag atagcattcc
     3901 tgaatcagag ttattagaag tggatcgtta cttgctaaat cgtttacgtg aatttactgc
     3961 aagtacgatt aacaactatg aaaactttga ctacttaaat atttatcaag aagttcaaaa
     4021 ctttatcaat gttgagttaa gtaatttcta tttggattac ggtaaagata ttttatatat
     4081 tgaacaacgt gattctcata tccgtcgtag tatgcaaaca gtgttatatc aaattttagt
     4141 tgatatgacg aagttgttag caccaatctt agtgcataca gctgaagaag tttggtctca
     4201 tacaccacat gttaaagaag aaagtgttca cttagcagac atgcctaaag ttgtagaagt
     4261 agatcaagct ttattggata aatggcgtac atttatgaat ttacgtgatg atgtgaaccg
     4321 tgcattagaa actgctcgta atgaaaaagt tattggtaaa tcattagaag ctaaagttac
     4381 gattgctagt aacgataaat ttaatgcatc tgaattctta acttcatttg atgcattaca
     4441 tcaattattt atcgtgtcac aagttaaagt tgtagataag ttagacgatc aggcaacagc
     4501 ttatgaacat ggtgatattg tcatcgaaca tgcagatggt gaaaaatgtg aaagatgttg
     4561 gaactattca gaggatcttg gtgctgttga tgaattgacg catctatgtc cacgatgcca
     4621 acaagttgta aaatcacttg tataattgaa attgtataaa gtactcatac agatgatata
     4681 aattaaagct ctcttcataa tcatgttgta gtttttgttg acatgatgaa gagagttttt
     4741 ttgtgaataa aaaaatgacc aagttaccgg tcatatatgt aaaaaatgtg cgatttacta
     4801 aaataaaaat tattcaggaa tggtacaaat tctctgaggc atataaatgc gttatagttg
     4861 ctattctcaa ttatgttcgc gataatttta agtaaaagta agcacagata ttgaatttga
     4921 taggagttaa ttgaatgtat cataacagta acgcaaactt tgtcaatggt atcactttaa
     4981 atgtgagaga taagaatgaa ttaaagccat tttatgagga catattagga ttaaatatta
     5041 taaatgagac attaacatcg atacaatatg aagtaggtca aaataatcat gtcattacac
     5101 ttgttgaatt acaaaatgga cgtgaacctt taatgtccga agcgggactg tttcatatcg
     5161 caattaaact acctcaaatt agtgatttag ctaatttact aattcattta agcgaatatg
     5221 atattccagt taacggaggt atacagcctg cttcgttatc attatttttt gaagacccgg
     5281 aaggaaacgg ttttaaattt tatgttgata aagacgaagc gcaatggacg aggcaaaata
     5341 atttagtaaa aattgatatt agaccatt
//
LOCUS       DANRDI010000170         5265 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.171, whole genome shotgun sequence.
ACCESSION   DANRDI010000170 DANRDI010000000
VERSION     DANRDI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.171"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            88..648
                     /locus_tag="PZ377_002428"
     CDS             88..648
                     /locus_tag="PZ377_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDM6359284.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            701..1630
                     /locus_tag="PZ377_002429"
     CDS             701..1630
                     /locus_tag="PZ377_002429"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDM6359285.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(1825..1998)
                     /locus_tag="PZ377_002430"
     CDS             complement(1825..1998)
                     /locus_tag="PZ377_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359286.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            2050..3429
                     /locus_tag="PZ377_002431"
     CDS             2050..3429
                     /locus_tag="PZ377_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDM6359287.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(3549..4577)
                     /locus_tag="PZ377_002432"
     CDS             complement(3549..4577)
                     /locus_tag="PZ377_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDM6359288.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            4847..5248
                     /locus_tag="PZ377_002433"
     CDS             4847..5248
                     /locus_tag="PZ377_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDM6359289.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
BASE COUNT         1804 a          880 c          816 g         1765 t
ORIGIN      
        1 tatatattaa ccattagggt taaaaattac tctagcattt atgaatagat gggagtttat
       61 tttattatta tataggagag atgttgaatg acacatcgcg cactattagt tgttgactat
      121 tcatatgact ttatcgcaga cgacggctta ctaacatgcg gtaaacctgg acaaaatatt
      181 gaagatttta ttgtttctcg tatcaatgac tttaattatt atcaagacca tatattcttt
      241 ttaatggatt tacattattt acatgacatt catcatcctg aaagtaaatt attcccacca
      301 cacaatatcg tagatacaag tggtagagaa ttatacggta aagtaggtaa attatacgaa
      361 acaattaaag cgcaacctaa tgtacatttc attgataaaa cgcgctatga ttcgttcttt
      421 ggtaccccgc ttgatagttt attgagagaa agaagtatta atcaagtcga aatcgttggt
      481 gtatgtaccg atatttgcgt gttacataca gcaatttctg catacaactt aggttataaa
      541 atttcagtac ctgctgaggg agtggcttca tttaatcaaa aagggcatga atgggcactt
      601 gcacatttca aaaactcatt aggtgcagag gtagaacaac acgtttaaat cgtgctaaaa
      661 taattataaa gaatacaatt tacaagggag atatttgaca atggctaaaa catatatttt
      721 cggacataag aatccagaca ctgatgcaat ttcatctgcg attattatgg cagaatttga
      781 acaacttcga ggtaattcag gagccaaagc ataccgttta ggtgatgtga gtgcagaaac
      841 tcaattcgcg ttagatacat ttaatgtacc tgctccggaa ttattaacag atgatttaga
      901 tggtcaagat gttatcttag ttgatcataa cgaattccaa caaagttctg atacgattgc
      961 ctctgctaca attaagcatg taattgatca tcacagaatt gcaaatttcg aaactgctgg
     1021 tcctttatgt tatcgtgctg aaccagttgg ttgtacagct acaattttat acaaaatgtt
     1081 tagagaacgt ggctttgaaa ttaaacctga aattgccggt ttaatgttat cagcaattat
     1141 ctcagatagc ttacttttca aatcaccaac atgtacacaa caagatgtta aagcagctga
     1201 agaattaaaa gatattgcta aagttgatat tcaaaagtac ggcttagata tgttaaaagc
     1261 aggtgcttca acaactgata aatcagttga attcttatta aacatggatg ctaaatcatt
     1321 tactatgggt gactatgtga ctcgtattgc acaagttaat gctgttgacc ttgacgaagt
     1381 gttaaatcgt aaagaagatt tagaaaaaga aatgttagct gtaagtgcac aagaaaaata
     1441 tgacttattt gtacttgttg ttactgacat cattaatagt gattctaaaa ttttagttgt
     1501 aggtgctgaa aaagataaag ttggcgaagc attcaatgtt caattagaag atgacatggc
     1561 cttcttatct ggtgtcgttt ctcgaaaaaa acaaatcgta cctcaaatca ctgaagcatt
     1621 aacaaaataa tactatatta ctgtctaatt atagacatgt tgtatttaac taacagttca
     1681 ttaaagtaga atttatttca ctttcaatga actgtttttt atttacgttt gactaattta
     1741 caaccctttt tcaatagtag tttttattcc tttagctacc ctaacccaca gattagtgat
     1801 ttctatacaa ttcccctttt gtctttacat tttcttaaaa tatttgcgat gttgagtata
     1861 aatttttgtt ttcttcctac ctttttcgtt atgattaaag ttataaatat tattatgtac
     1921 acgattcatc gctctatttt caactttcaa catatataat tcgaaagacc atttaaaatt
     1981 aacggccaca acattcaaat caattaatcg ctttttccaa aataatcata taaggaggtt
     2041 cttttcatta tgaatatcat tgagcaaaaa ttttatgaca gtaaagcttt tttcaataca
     2101 caacaaacta aagatattag ttttagaaaa gagcaattaa agaagttaag caaagctatt
     2161 aaatcatacg agagcgatat tttagaagca ctatatacag atttaggaaa aaataaagtc
     2221 gaagcttatg ctactgaaat tggcataact ttgaaaagta tcaaaattgc ccgtaaggaa
     2281 cttaaaaact ggactaaaac aaaaaatgta gacacacctt tatatttatt tccaacaaaa
     2341 agctatatca aaaaagaacc ttatggaaca gttttgatca ttgcaccatt taactatcct
     2401 tttcaactag tgttcgaacc tttaatcggt gctattgcag caggtaatac ggcaattatt
     2461 aaaccatctg agttgacgcc taatgttgca cgagtgatta aacggttaat caatgaaaca
     2521 tttgatgcaa attacattga agttattgag ggaggaattg aagaaacgca aacgttaatt
     2581 cacttacctt ttgactatgt cttctttaca ggaagtgaaa atgtaggcaa aatcgtttat
     2641 caagctgcca gcgaaaattt agttcctgtg acattagaaa tgggcggtaa atctccagtc
     2701 atcgttgatg aaacagcgaa tattaaagtt gctagtgagc gcatttgttt tgggaaattc
     2761 actaatgccg gccaaacatg tgttgcacca gattacattt tagtacacga atctgtaaaa
     2821 gatgatttaa tcacagccct atcaaaaacg ttgcgtgaat tttatggtca aaatatacaa
     2881 caaagtccag attatggccg cattgtaaac cttaaacatt atcatcgtct gacttcatta
     2941 cttaacagtg cacaaatgaa tattgtattt ggtggtcata gtgatgagga tgaacgttat
     3001 atagaaccaa cattgttaga tcacgttaca agtgattcag caattatgca agaagaaatt
     3061 tttggtccta tcttaccgat tttaacgtat cagtcattgg atgaagcaat agcctttatt
     3121 caccaaagac caaaaccttt gagtttatat ttatttagcg aagatgaaaa tgctacacaa
     3181 cgtgtaataa acgagctatc atttggcggc ggcgctatta atgatacatt gatgcaccta
     3241 gcgaatccta aattaccatt tggtggtgtt ggcgcctcag gtatgggacg ctatcatggt
     3301 aaatattcat tcgacacttt tacacatgaa aaaagctaca ttttcaaatc tacacgatta
     3361 gaatcaggtg tccatttacc accatataaa ggtaaattta aatacatcaa agctttcttt
     3421 aaaaattaat ttttgagaca aaaataaacc atgcttccat aatttgatgt tcttgaaaac
     3481 aataaatttc aaggtataca caaatttttt ggaaacatgg ttttcttttt atcatgatta
     3541 attaaagatt aaaatatgac acatacacct tcagatgcta cacgagtgtt atcacatagc
     3601 gtaattttac ctgtttcttt atttctttcg aaaacagtta caactgaatc tccttgctca
     3661 tgagcacaaa ctaaaaggtc atctgatgag gcaatattaa aatctcttgg gaattgacca
     3721 ccactttcag taattgttac tagttctaag tgttgaccat tatcaagaac tttaaaaatt
     3781 gcaatgctat catgccctct attagataca tataagaatt gttgatcatg agataaacgc
     3841 actgctgcaa gtttagtatc tccatcaaag ttttcaggaa ttgttaaatg acgctcgagc
     3901 tcttcaaatt taccgtcatt atattctgca acactcacag tatttgataa ttcgtgtacg
     3961 acataagcaa attttccatt atcatgaaat tcaatatgtc ttgtcccatc actatcttta
     4021 aataaagatt ctttataaaa ttcaaacccg ttgtcatcaa atttataagt aacgatacga
     4081 tcagcaccta aatctgttac tgcaacgtac ttaccatctg gagtttgatt aatataatgt
     4141 gcgtgtggat gatcttgtct ttcatgtgta cctgttggaa aatcatgtgc aagttcttga
     4201 attagacgta taatttcacc tgtgtgcgta tttaattcat acatacgtat gatgccagca
     4261 ccatataccg cttcaaataa atatcgttta tcttctgaaa tcgatacata acaacctgta
     4321 ccagcttttg aagacaaaca tttatttaat aaatgcaatt caccattgtc atcaattttc
     4381 aaacttgcaa caccgcattg ttctccttct ttgttgattc catataaaac ttcattatta
     4441 cgcaccaaat atgtagacgc ttctaattca aatcctgttt ctaataaatc aatacgtgac
     4501 tgattttcgt ttaattcaaa acgataaatc cctttaccat tctttttagt gtaagaacca
     4561 atatatccat ttgtcattac ttttacccct ccatgttagt cttattttca ttttattaaa
     4621 ttttagctag aattaacaat catttatcat ttccttaaag ctaaaactat ttaactactt
     4681 taaaagaact atttgctgtg atatgtcatt attcgatttt aattaatccc tcttttttac
     4741 ttacacgagc attaattatt aacgcgttta tgcttccaca aaatttttgt tgcaaaatag
     4801 aacacatgtt cgtataatgt aattatcaga ttggggtgaa ttaaggatga caagttatga
     4861 agaagaaact gattatcgta acgttccaag agaattacta gataacaata tacctatggg
     4921 aagaggcatg attaaatggg ctccttttgc aacattaccc gaacaattcg aaacgattca
     4981 acaatacatt atcgaccaaa acaaaattac acgtcctgta ttatcagatg atcaattggc
     5041 tgagctaaac attcggctac acgaggcgct acaatatgcc caacctgtag aagttaaatt
     5101 ttataataat ggctttgtag attcagtacg cttaaccatt tatcgtattg atgctattaa
     5161 ttatgaaatt gatggttatg tttacaatca gcaacaacgt caaaaaattt ctatctttga
     5221 tatcttagat attgtcttgt taccttaatg ctttgaacat aatta
//
LOCUS       DANRDI010000171         5260 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.172, whole genome shotgun sequence.
ACCESSION   DANRDI010000171 DANRDI010000000
VERSION     DANRDI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.172"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(515..1540)
                     /locus_tag="PZ377_002434"
     CDS             complement(515..1540)
                     /locus_tag="PZ377_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDM6359290.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            1810..2007
                     /locus_tag="PZ377_002435"
     CDS             1810..2007
                     /locus_tag="PZ377_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359291.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEATNLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            2065..3546
                     /gene="cls"
                     /locus_tag="PZ377_002436"
     CDS             2065..3546
                     /gene="cls"
                     /locus_tag="PZ377_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDM6359292.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            3721..4629
                     /locus_tag="PZ377_002437"
     CDS             3721..4629
                     /locus_tag="PZ377_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359293.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
     gene            4598..>5260
                     /locus_tag="PZ377_002438"
     CDS             4598..>5260
                     /locus_tag="PZ377_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6359294.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDV
                     KPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVK
                     TMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLIS
                     FAI"
BASE COUNT         1903 a          750 c          777 g         1830 t
ORIGIN      
        1 tgctattttt tgcgctttta taacaactaa ggatacaaga cacataactt caacaatatc
       61 ctcatctaaa cctatcgatt tcctaagctc aattttaaac acaaagttta tttgaaatga
      121 tgcacaccta taaaaatctt aatcatcgaa aagtcacaaa catctccata ttaaaaactt
      181 aaatagtgca ttccatttaa actaaacatc gatattccta ccaatataga cttagtaatt
      241 cgattgttaa tacattactt tcataaaata actacaacca tcataatact tctcaaatgt
      301 cgtcaaatta aagtaaatac agcacaaatt aacattttta aatatataaa ataaaacaag
      361 atactcaata atctgtagta ccttgttttg gaataatgta attttatatc atcaacggct
      421 acaaatgagc agtttcatat ataaataaac acatctactc aactgatctt aattaattaa
      481 taaatgattt accagatgac atataacagc attattagat taattctaat agtttattta
      541 aattttcttc ggttgtcgcc caactggttg cgaatctaac aacacgatgt tgatcatcgt
      601 atttttccca aacagcaaat ttaacttttt gttctaactc tgctattttc tcgttactta
      661 aaataaaaaa ttgttgattg gttggagaat caaagtaaag acgatagcct ttatttttaa
      721 acccgtcttt catcttattt gccatttcga tagcatgtct gcttatatta aaatataaat
      781 tgtccgtaaa taattctaaa aattgtatgc ctgttaaccg tccttttgct aaaagggcac
      841 cgtgatgctt gattcgagtg gtaaattgtt tcggttcatt atttttcgta aaaacaatgg
      901 cttccccgca taatgcacct atcttcgtac cacctatata aaatacatca caatatttag
      961 cgatgtcttt aatagtcata tctgattggt cactcatcaa tccataccct aatcgtgcac
     1021 catccataaa taatggaagc tgatattgct tacatacttt gcataactct tccaattctg
     1081 atttagagta taatgtgcca tattctgtag gatgagaaat atataccatt cctgggaata
     1141 ccatatggtc ctttttaaaa tcacttttaa atgtctccat gtaagtttca acatctgaag
     1201 cactaacttt tccttcctta gagggtatag taattacttt atgtccacta tattcaattg
     1261 caccgccctc atgcacagca acatgaccag tgtctgctga aatgacccct tcgtaacttt
     1321 ctaacattga attaataaca acctgattgg tttgtgttcc acctactaaa aaacgaattg
     1381 tagcatttgg acagtcaatt gtatctttaa tcttttcaat tgcctgagct gtgaattgat
     1441 caaagccata tcccgaagct tgtacaagat ttgtatctac taatcgtttt aatacttttt
     1501 catgagcacc ttctaaataa tcattttcga atgaaatcac tacatttccc cctaaaacta
     1561 atatcaacat tttaataaga taaaccaatt tcaaaactag ttcgatattt aaaatgtatt
     1621 atgaatggtt aaagtttgta tcgcattatc gcgaagttga ataaatatat tatacatcaa
     1681 acaatacatt atgaaaatta attaattttt tatcacagcg acttttcatg ctggaacaca
     1741 tgtattaatg attgaatttt actgttaaaa gttctatgat ttaggaaata gagaaaagag
     1801 gggatttaaa tggaacaaat taaacttaaa acttttacag ctgagacttt agagttatta
     1861 gaaaaaaaca ttaatgcttt tttaagttct gaagaagcta caaatttaaa attagtaaat
     1921 attactatta aagaaataga agaaagaaca ttcccaaata atgaagaaga attcaatgca
     1981 attttaactt tatctgtgaa taaataatat gacgtgaaag gtgcaaaatg tttttttgtg
     2041 cctttcttta taggagaaaa gactatgcga tttacatttt caaacgattt aggaacgtta
     2101 tttactatta ttttagccat tggattcatc attaatttag tattggcttt tattattatc
     2161 tttttagaaa gaaataggcg tacagcgagt tcaacttggg catggctatt tgtacttttt
     2221 gtcttaccat tgattggttt tattctttac ttgttttttg gtagaaccgt ttcggcacgc
     2281 aaattgaata aaaacaatgg taacgtgtta acggatttcg atggactttt aaaacaacaa
     2341 atagaaagct ttgataaagg taattatggt actgataaca aacaagttca aaaacatcat
     2401 gatttagtac gtatgctttt gatggatcaa gatggttttt taactgaaaa taataaagtt
     2461 gatcatttca ttgatggaaa tgatttatat gatcaagttt taaaagatat taaaaatgca
     2521 aaagaatata tccatttaga gtactatact ttcgctttag atggtttagg taaaagaatt
     2581 ttacatgctt tagaagaaaa attgaaacaa ggtctagaag taaaaatatt atatgatgat
     2641 gttggatcta aaaatgttaa gatggcaaat tttgatcatt ttaaatcgtt aggtggagaa
     2701 gttgaagcat tttttgcttc aaaattaccg ttattgaatt tcagaatgaa taatagaaat
     2761 catagaaaaa tcatcgtaat cgatggtcaa ctaggttatg tcggaggatt taacattggt
     2821 gatgaatatc taggattagg aaaattagga tattggagag atacgcattt acgtatacaa
     2881 ggggatgcgg ttgatgcact gcagttgcga tttattttag actggaattc gcaagcgcac
     2941 cgtccacaat ttgaatatga tgttaagtat ttccctaaaa agaacggacc attgggcaat
     3001 tcaccaattc aaatagctgc aagtggcccg gctagtgact ggcatcaaat tgaatacggt
     3061 tatacaaaaa tgattatgag tgcaaagaaa tctgtatatt tacaatcacc atatttcatt
     3121 ccggataatt catatataaa tgccattaaa attgctgcta aatcaggtgt agatgtacat
     3181 ttaatgattc catgtaagcc agatcatcca ttagtatatt gggcgacatt ttcaaatgcc
     3241 tctgacttat tatcaagtgg tgttaaaatt tatacgtatg aaaatggatt tatacattct
     3301 aaaatgtgct taattgatga tgaaatcgta tcagtgggca cagcaaatat ggactttaga
     3361 agttttgaat taaattttga agtaaatgcc tttgtatatg atgaaaatct tgctaaagat
     3421 ttaagggtgg cttatgaaca tgatattaca aaatcaaaac aactaaccaa agaatcatat
     3481 gccaatagac cgctgtctgt taaattcaaa gaatcgttag caaaattagt ttcgccaatt
     3541 ttataattta tttgtaagga gtctcgatta tagaggctct ttttattttg cccaaaatta
     3601 aaataacgat aaatatatta tgtaaatcta ataatgacat ttgtcatttc aaatctatga
     3661 caaagtgttc tattttaaat ctaataaatt tactaatatt aaattaagaa aaaaatagag
     3721 gtgattaaat tgattcaaat atctaatatc aacaagtcat ttaataaaag atgtgttcta
     3781 aaaaatattt cgttcgatat tgaacaaggt aaatgtatcg ctttaattgg aaaaaatggt
     3841 gctggaaagt caacgttaat tgatatatta attggtaatg ttaatgctaa ttctggtgag
     3901 atatttgata aagacaagtt attacaaagt gaaaatcgca gtataatgtt ccaaaaaacg
     3961 atgtttccag atcaattaaa agttattgag attatcaact tatatcaatc attttacgaa
     4021 aatccattac cattggaaga aataatagaa ctgacgaaat ttgattctag tcaactgaac
     4081 caatttgtaa ataaactttc tggtggtcaa caacgattac tcgattttgt attatcttta
     4141 atcggacaac cacaattgat cttattagat gaaccaacat cgactatgga tatagaaatt
     4201 agagaatatt tttggtcaat tattgaaaat ttaaaagaag ataatcgaac gatactctat
     4261 acatcgcact atattgaaga agtcgaacgt atgtcagaca aaattattct cattgaaaat
     4321 ggagaaataa tacttaatga ttcaacgtca catattagaa ccaatcagca atctcagatt
     4381 acgttatccg atgaatatat aagaaagtta aaactagata aagatgattt agttattcaa
     4441 aaaaatcata atggcactat caaaattatt acttcaaatg taaatgatac gattttatat
     4501 cttcaacaac ttcatattaa tttggatgat attgaaatac aaaaagtctc aattgttgat
     4561 tcatacttca acaataaaaa gcaaagggga tctaattatg atactaagtt acttgaaaat
     4621 cgaatttaaa gttataatgc gtaaaaaaac aacattaata ttatctattt tatttcctgt
     4681 tatattctat atattattta cttcgatatt ggaattgccg gaagatgtta aacctaaatt
     4741 ttataaagag tatatgtata gtatgacggt ttatagtttg ttaagtttta gtttactaac
     4801 ttttccatta gatattatta atgaaaaaca aaatgaatgg cgccaaagat taatggtaac
     4861 accatttact tttactagtt attatatttc aaaagtagtg aaaactatgc tgcaatttgc
     4921 aatagcgata ttagttattt ttatggttgg acatttttat aaaggtgttg caatgagtgc
     4981 agttcaatgg ttagagtcag gaatattttt atggttaggt gcgtctctat taataacttt
     5041 tggcatatta ttttctttgt taaatgatat tcaaaaaaca agtgctttag ctaatatcgt
     5101 aacaattggt ttagcagtat taggtggatt gtggtttccg ataaacacat ttccaaattg
     5161 gcttcaacat gttgctcatg ttttaccgag ctatcatttg cgtaaactag gtgtagatat
     5221 tgcttcaaat catcatatca atttaatatc atttgctata
//
LOCUS       DANRDI010000172         5213 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.173, whole genome shotgun sequence.
ACCESSION   DANRDI010000172 DANRDI010000000
VERSION     DANRDI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.173"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..355)
                     /locus_tag="PZ377_002439"
     CDS             complement(<1..355)
                     /locus_tag="PZ377_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDM6359295.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSY"
     gene            complement(361..816)
                     /gene="bcp"
                     /locus_tag="PZ377_002440"
     CDS             complement(361..816)
                     /gene="bcp"
                     /locus_tag="PZ377_002440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDM6359296.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            897..2186
                     /locus_tag="PZ377_002441"
     CDS             897..2186
                     /locus_tag="PZ377_002441"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDM6359297.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            2479..3573
                     /locus_tag="PZ377_002442"
     CDS             2479..3573
                     /locus_tag="PZ377_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDM6359298.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(3663..3755)
                     /locus_tag="PZ377_002443"
     CDS             complement(3663..3755)
                     /locus_tag="PZ377_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359299.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(3764..>5213)
                     /locus_tag="PZ377_002444"
     CDS             complement(3764..>5213)
                     /locus_tag="PZ377_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDM6359300.1"
                     /translation="RKKLYNHLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLM
                     NIWLDCITIIIALSIMFFLDVKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAE
                     VQGFLHERVQGISVVKSFAIEDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTD
                     IGPIIVIGVGAYLAISGSITVGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRV
                     FQLIDEDYDIKNGVGAQPIEIKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAF
                     VGMSGGGKSTLINLIPRFYDVTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDT
                     VKENILLGRPTATDEEVVEAAKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSI
                     ARIFLNNPPILILDEATSALDLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIV
                     VIENGHIVETGTHRELIAKQGAYEHLYSIQNL"
BASE COUNT         1733 a          889 c          790 g         1801 t
ORIGIN      
        1 catatgatag tttcatcttt ttataatcat ctaaaatgaa agctaaaatg tattcagata
       61 attgtttagc ttgaacacct tttccattag ttaaaagtat gccgtgatct gcaatataat
      121 ctaacggcaa tgtatttaca cccgttgcaa accatgcaat ccattttaaa ttcgggcaac
      181 gtcgtaaaaa tgcctcattg ataccgccat cataaccaac taatatatct aaatcagcca
      241 agtcgctctc aggtatttct gatgcttttt tataaaattt aaaatctaaa tctgaaaagc
      301 gttgttgtaa ttcagtttca acttcacgca tacgatttaa cccaacaact ttcatatatg
      361 tcaccccaaa atgtttttaa gttcttctat ttgtgtttta accttaacct tctcgataac
      421 atctaatact ttaccttgtt catctattat aaaagtcgtt cttacaatgc ccatactttc
      481 tttgccaaat gattttttta actgatatac gccagtttct ttagctaatt taaaatcttc
      541 atctactaat aaatcgaaat tcaatccgtg tttctcaata aaattttggt gttttttctt
      601 tgaatcaccg cttataccat atactgcaac atctaaatca ttgaacattt ctaaattgtc
      661 tctaaagtca caagcttctg tggtacaagt aggtgtatta tctctaggat aaaaatatat
      721 aatcgccttt ttacctttta atgtatcatt tgtaatgaca gttccgtctt gattttctaa
      781 tttaaatatt ggaaattgtt ctcctttttg caacatataa tcaccttttt cttcattata
      841 ataattttat gatacgatat tagatgaaaa tattaaatta aaagaggttg atataaatga
      901 attttagtga aagtgaacgt ttacaacaac tttcaaacga atatattcta ggcggtgtca
      961 attccccttc tcgttcttat aaagctgtag gaggcggtgc acctgttgtt atgaaagaag
     1021 gacacggtgc atatttatat gatgtcgatg gcaataaatt tattgattac cttcaagcat
     1081 acggtccaat tattacgggg catgcacatc ctcatattac taaagcaatt caagaacaag
     1141 ctgctaaagg tgttttattt ggtacaccga ctgaattaga aattgaattc agcaaaaaat
     1201 tacgtgatgc aattccatct cttgagaaaa ttcgctttgt aaattctgga acagaagcag
     1261 tcatgacaac aattcgtgtt gcacgtgcat atactaaaag aaataaaatt ataaaatttg
     1321 ctggatctta tcatggccat tctgatttag tattggttgc agcaggtagc ggcccatctc
     1381 agctcggttc tccagactca gctggtgttc cagaaagcgt cgcacgtgaa gtcattactg
     1441 tacctttcaa tgatattaac gcctataaag aagcaattga attttggggt gatgaaattg
     1501 ccgcagtatt agtagaacca attgttggta actttggaat ggtaatgcct caacctggat
     1561 ttttagaaga ggttaatgaa atttcacata acaatgggac cctagtgatt tatgatgaag
     1621 taattactgc attccgtttc cattacggtg ccgctcaaga tttattaggt gttatccctg
     1681 atttaactgc atttggtaaa attgttggcg gtggtttacc aattggaggc tatggtggac
     1741 gtcaagatat tatggaacaa gtagcacctc taggacctgc atatcaagct ggtacaatgg
     1801 ctggtaaccc gttatctatg aaagcaggta ttgcattact cgaagtacta gaacaagacg
     1861 gtgtttatga aaaattagac agcttaggcc aacaactaga agaaggttta cttaaattaa
     1921 tcgaaaaaca taatatcaca gctacaatta atcgtattta tggatcttta acattgtact
     1981 ttacagatga aaaagtcaca cattatgatc aagttgaaca ttctgacggc gaagcgttcg
     2041 gtaaattttt caaattaatg ttaaatcaag gtatcaattt agcaccttct aagtttgaag
     2101 cttggttctt aacaactgaa catacagaag aagatattaa acaaacttta aaagctgcag
     2161 actatgcttt tagtcaaatg aaataacttt taaggaagta tatttttaat tgaattaaaa
     2221 taaagcaaat gctcacatag tagattcatt ttatatcgtt aactattaac gtttaacttg
     2281 atatgtcact atgtgagttt ttattttaca aatttatact attttaatct ctaactaaag
     2341 ttttacatcg ctatcagtat tatgtatgat ttattttaac aactataaat aagcttgaat
     2401 tttgtaacta gtaagtgtat aacagtaatg aatacgaata ttagatattc aataaactta
     2461 aaaggggcgt cattattttt gagactagga gctcggattt ttaaaactgg tatagccatt
     2521 attttagcta tgtctatcgc ttctttacta ccggatgatg ttggtctgaa agccttagct
     2581 ggtgtcagtg ctgttgttgc aatgcaacct agtatttatc gttcatttaa aacagtttct
     2641 gatcaagcat tgggtaacat tataggtgcc atactatctg ttacaatggt aacaattttt
     2701 agtgataatt ttatcattat gggtgttacc gtcatagtac ttattgctat tttatttaaa
     2761 tttaatttag cacatgttgc tactttggcg agtgtaacag cattaatcat tatgggacaa
     2821 cataccggtt ctttctatat taccgccttt tacaggtttg tacttgtaat gattggtgtc
     2881 ataagttcat ctctagttaa ctttgtgttt ctaccaccta aatttgaaac aaaaatttac
     2941 tataattcat taaatatatc ctcagatatt tttatgtggt ttaaattagt attaaatgat
     3001 acgacggagt ttaataatat taaacaagat agtcataatt taaaacaacg tgtcgaaaag
     3061 ttagagaaaa tttatgatta ctatagcgaa gaacgtccta ttacaaaaaa acatattcac
     3121 caacagaata gaaagaaaat acttttcaga gaagtagttc agacgactag acaagcttat
     3181 gaagtattaa acaaattgtc gcgttatcaa aacgacttat atttacttaa taacaatttc
     3241 ctattacaaa tcaaattaga tttagattca ttaactgctt ttcacgagca aattttagct
     3301 agcctttcta aaaaagcacg ttataatgtg acacatgttg attatgagtt agataatccg
     3361 cagaaaaaag atttattgtc cacatttcag catgagttaa taaatcatcc ttatcaaacg
     3421 gaatattcgt ttgccaatgt tatgcaaatt gttgcagcca tcgaagagta tcgacaccat
     3481 ttagagcatt tagaccgcat aagaattagc tttttcacat atcatagatc tgatgcagac
     3541 attgaaattg ttgaagaaga ctttgattta taacattaaa acaattttat caattaatgc
     3601 cacaatgaga taaactaata ttgaatctcg aaattaaatt taaaaaagca gtaagattat
     3661 tttcaattaa gaaaatctct tactgctttt tctatgttat gaccccacca tcctgttaca
     3721 aattttctct tcaagattgt actgaagtca aacaactaat actttataag ttttgaatgc
     3781 tatataaatg ctcgtaagca ccttgttttg caatcaattc acgatgcgta cctgtttcaa
     3841 caatatgtcc attttcaatt acgacaattt tgtcagcatg tgtaatagtg gacaagcgat
     3901 gcgctacgat aagtgtcgtt cgatctttac tcaacacatc taatgcttct tgaataatgg
     3961 attcactttc taaatcaagt gcacttgttg cttcatccaa gataagaatt ggcggattat
     4021 ttaaaaatat tctagcaatc gataatcttt gtttttgacc acctgataat ttaacacctc
     4081 gttcacctac ttcagtgtca tatccttgtg gcaagttcat aataaagtca tgtgcattag
     4141 ccattttcgc cgcttcaact acttcttcat ctgttgctgt tggacgacca agtaaaatat
     4201 tttccttaac tgtatcagag aataaaatat tatcctgttg caccaatcct atttgatttc
     4261 ttaaacttcc cgttaaaaaa tctttaatgt tgtgaccatc tattaaaatt tgcccagaag
     4321 ttacatcgta aaatctcggt attaagttaa ttaatgttga tttaccacca ccactcatac
     4381 ctacgaaagc aactgtttct cctttttcaa tactcaaatt aatatctttt aaaattggag
     4441 cttcgttatc gttatattga aaactaacat gatcaatatc aatacgacct tgtttaattt
     4501 caataggttg agcaccaaca ccatttttga tgtcataatc ttcatcaatt aattggaata
     4561 cacggtccat tgaagcaaaa ctttgcgtta aagttgtaaa tgatgcgact aaacgacgta
     4621 aaggcccgaa caataactct aagtatccaa caaatgctgc aagtgtacct actgtgattg
     4681 atccagaaat agcaagatat gcaccaacac cgatgacaat aattggtcca atatctgtaa
     4741 ctgtattaat tgcggcaaag gaataggcat tccatcttgt atgtttcaac gcacgtgtta
     4801 ggaaattagt attcttttta tcaaagtttt tcgcttcatt gtcttcaatc gcaaaacttt
     4861 taacgactga aataccttga acacgttcat gcaagaatcc ttgaacctca gctaatgctt
     4921 gagatctttc acgtgtcaat tttcttaatc ttccaaagaa aacgtacacc gttaaaatgt
     4981 aaaatgggaa gataaacagt gctgctaaag tcaatttcac atctaaaaag aacattatgg
     5041 atagtgcaat aataattgtt atacaatcta accaaatatt cattaacccg gttaaaatga
     5101 aatcttttgt ttgttcaaca tcattaatca ctctagatat tacttgacct acttgattat
     5161 tagcataaaa tctcgcactt aaagcttgta aatggttgta taacttttta cgt
//
LOCUS       DANRDI010000173         5170 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.174, whole genome shotgun sequence.
ACCESSION   DANRDI010000173 DANRDI010000000
VERSION     DANRDI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.174"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            71..1171
                     /gene="carA"
                     /locus_tag="PZ377_002445"
     CDS             71..1171
                     /gene="carA"
                     /locus_tag="PZ377_002445"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDM6359301.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            1164..4337
                     /gene="carB"
                     /locus_tag="PZ377_002446"
     CDS             1164..4337
                     /gene="carB"
                     /locus_tag="PZ377_002446"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDM6359302.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            4447..5139
                     /gene="pyrF"
                     /locus_tag="PZ377_002447"
     CDS             4447..5139
                     /gene="pyrF"
                     /locus_tag="PZ377_002447"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDM6359303.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
BASE COUNT         1859 a          778 c         1038 g         1495 t
ORIGIN      
        1 tataaagttt atggaaatcc gatcttaaca atggttgaag gcgaagttaa atttgagggg
       61 gataaataat atgcaaagca aacgttatct agtgttagaa gacggttctt tttacgaagg
      121 ctaccgttta ggatctgata acttaactgt aggagaaatt gtatttaata cagcgatgac
      181 aggttatcaa gaaactattt cagatccatc atatacaggt cagatcatta cttttacgta
      241 tccattaatc ggtaattatg gtatcaatag agacgatttt gaatcattag tacctacatt
      301 aaacggtatt gtagtgaaag aagcgagtgc gcatccaagt aattttagac agcaaaagac
      361 acttcatgac gttttagaat tgcatcaaat tccagggatt gcaggtgttg atacaagaag
      421 tattacgcgt aaaattcgac aacacggtgt gttaaaagct ggttttactg atcgaaaaga
      481 agatattgat caacttgtca aacatttaca acaagtagaa ttacctaaaa acgaagtaga
      541 aatcgtttcg actaaaacac cgtatgtttc gacaggtaag gatctaagtg tcgtacttgt
      601 agactttggt aagaagcaaa atattgttcg agaattaaac gtcagaggtt gtaacgtcac
      661 agttgtacca tatacaacta ctgccgaaga aattttagca atggctccag atggcgttat
      721 gctatcaaac ggaccaggta atcctgaagt tgtagaatgt gcgattccaa tgattcaagg
      781 aattttaggg aaaattccgt tctttggtat ctgtctagga catcaacttt ttgcattatc
      841 tcaaggagca agctcattta aaatgaagtt tggtcatcgt ggtgcgaacc atccagttaa
      901 aaatttagag actggaaaag ttgatattac gagtcaaaac catggatatg caatagatat
      961 agattcgtta aaaagtactg atttagaagt tactcatctt gcattaaatg atggtactgt
     1021 agaaggttta aaacataaaa cattaccagc attttctgtt caataccatc ctgaagcaaa
     1081 tccaggaccg tcagattcaa actatctatt tgatgatttt gtagcaatga tgactaattt
     1141 taaggaaaag gagcgtcata tcaatgccta aacgtaatga tatcaaaaca attttagtaa
     1201 tagggtctgg gccaattatc ataggtcaag cagctgaatt tgattatgct ggaacacaag
     1261 catgtctagc tttaaaagaa gagggatatc gagttattct tgtaaattca aatccagcga
     1321 caatcatgac tgataaggaa attgcggata aagtatatat cgaaccgtta actcatgatt
     1381 ttatagcgcg aattatacgt aaagagcaac ctgacgcttt acttccaact ttaggtggtc
     1441 aaacaggttt aaacatggcg attcaactac acgaaagtgg tgtgcttcaa gataataacg
     1501 tccaattatt aggaactgag ctaacatcaa ttcaacaagc agaagaccgt gaaatgttta
     1561 gaacattaat gaatgattta aacgttcctg taccagagag tgacattgta aatacagtag
     1621 agcaagcctt taaattcaaa gagcaagtgg gatacccgct aattgttaga ccggcattta
     1681 cgatgggtgg taccggaggc ggtatttgtc ataatgatga agaattacat gaaatcgtct
     1741 caaatggtct tcattatagt ccagcaacgc aatgtttatt agaaaaatct atcgcaggtt
     1801 ttaaagaaat cgaatacgaa gtaatgcgtg ataaaaacga taatgccatc gttgtatgta
     1861 acatggaaaa tattgatcca gttggtattc atacaggcga ttcaattgtt gtggctccta
     1921 gtcaaacatt atcagatgtt gagtatcaaa tgttacgtga tgtttcatta aaagttattc
     1981 gagctttagg tatcgaaggt ggttgtaatg ttcaattagc attagatccc cattcattcg
     2041 attattatat tatagaagta aatccgcgtg tatcacgttc atcagcgtta gcttcaaaag
     2101 caacaggata tcctattgca aaattagctg ctaaaatcgc ggttggtcta acattagatg
     2161 aaatgttaaa tccaattaca ggaacatctt atgcagcgtt tgaaccaact ttagactatg
     2221 tgatttcaaa aataccaaga tttccttttg ataaatttga aaaaggagaa cgagagcttg
     2281 gcacacaaat gaaagcaaca ggtgaagtta tggccattgg tcgaacttac gaagaatcat
     2341 tgttaaaagc aattcgatca cttgagtatg gtgtgcatca cttaggatta ccaaatggtg
     2401 aaagcttcga tcttgattat attaaagaac gtatttcaca ccaagatgat gaacgattat
     2461 ttttcatcgg cgaagcaatt agaagaggca caacattaga agaaattcat aatatgactc
     2521 agattgatta cttcttctta cacaagttcc aaaacattat tgatattgag catcaattaa
     2581 aagagcatca aggtgattta gaatatctta aatatgcaaa agattatgga tttagtgata
     2641 aaacaatagc gcatcgcttt aatatgacgg aagaagaagt atatcaattg cgtatggaaa
     2701 atgatattaa acctgtttac aagatggttg atacttgcgc agctgaattt gaatcttcaa
     2761 caccatatta ttatggtaca tacgaaactg aaaatgaatc catagttact gacaaagaaa
     2821 aaatcttagt attaggctct ggaccaattc gaatcggcca aggtgtagaa tttgactatg
     2881 cgacagttca cgccgtttgg gcaattcaaa aagcagggta cgaagcgata attgtgaata
     2941 acaatccaga aacagtttca acagacttct caatttctga caaattatac tttgaacctt
     3001 taactgaaga agatgtgatg aatatcatta atttagaaaa acctaaaggt gtcgttgtac
     3061 aatttggagg acaaacagcg attaatttag cagacaaatt ggctaaacat ggtgttaaaa
     3121 tacttggtac ttcactagaa aatctaaatc gtgctgaaga tagaaaagaa tttgaagcac
     3181 tattaagaaa aattaacgtg ccacagccac aagggaaaac agctacatca cctgaggaag
     3241 cattagcgaa tgctgcagaa atcggatatc cggttgtagt aagaccttct tatgtattag
     3301 gtggtcgcgc aatggaaatt gtagacaatg acaaagagtt agaaaactat atgacccagg
     3361 ctgtaaaagc gagtccggaa catccggtac tagtcgatag atatttaact ggtaaagaaa
     3421 ttgaagttga tgcgatttgt gatggagaaa cggtcattat tccaggaatc atggaacata
     3481 ttgaacgtgc tggtgtgcat agtggtgact caatcgctgt atatccacca caaactttga
     3541 cagaagacga gttagcaaca cttgaggact atactataaa attagctaaa ggtttaaaca
     3601 tcattggctt aatcaacatt caattcgtta tagctcacga tggtgtgtat gttttagaag
     3661 taaatccacg ttctagtaga acggtaccat tcttaagtaa aattactgat attccaatgg
     3721 cacaattagc tatgcgagca atcattgggg aaaaactaac agatatgggt tatcaagaag
     3781 gggttcaacc atatgctgag ggtgtctttg tgaaagcacc agtatttagt tttaataaat
     3841 tgaaaaatgt tgatattact ttaggacctg aaatgaagtc aacaggtgaa gtgatgggga
     3901 aagatactac attagaaaag gcgttattca aagggttaac aggtagtggc gttgaagtta
     3961 aagatcacgg tacagtatta atgaccgtca gtgacaaaga taaagaggaa gttgttaaat
     4021 tggcacaacg cttaaatgaa gttggctata aaattttagc aacgtctgga acagctaata
     4081 aattagctga gtatgacata cctgcagaag tagtaggcaa aattggtggc gaaaatgatt
     4141 tattaacacg tattcaaaat ggtgatgttc aaatcgttat aaatacaatg actaaaggta
     4201 aagaagtaga aagggatggc ttccaaatta gacgtactac agttgaaaat ggtattccat
     4261 gtttgacatc tttagataca gctaatgcct taacgaatgt aattgaaagt atgacattta
     4321 caatgcgtca aatgtaaatc aatcaaactg tatcggtggg gctgtaatta accatttact
     4381 taaagaagtt tatattacag cctcattatt ttaatgaatt tcttaatata aagggagaca
     4441 tatatgatga aagatttacc aattattgca ttagattttg aatcaaaaga aaaagtaaat
     4501 caatttttag atttatttga tgaatcatta ttcgtaaaag taggtatgga acttttttat
     4561 caagaaggtc ctcaattaat taatgagata aaagaaagag gccatgatgt atttttagat
     4621 ttaaaactgc atgatattcc taatacagtt ggtaaggcga tggaaggact agctaaattg
     4681 aatgttgatc tggtaaatgt tcatgctgct ggtggcgtaa aaatgatgtc tgaggccatt
     4741 aaaggattaa gaaaacataa tcaagataca aaaattattg cagtaacaca gcttacgtca
     4801 acaacagaag acatgttacg acacgaacaa aatatacaaa catcgattga agaggccgtt
     4861 ttaaattatg ccaagttagc aaatgcagct ggtttagatg gcgttgtttg ttcacctctt
     4921 gaaagtcgta tgttgactga aaagttaggt acatcatttt taaaagtaac accaggtatt
     4981 agacctaaag gtgcatctca aaatgaccaa caccgtatta cgacaccgga agaagcaaga
     5041 cagcttggtt cgacgcatat tgtagtcggt agaccgatta cacaaagtga caatccagtc
     5101 gaaagttatc ataaaattaa agaaagttgg ttagtataat ggctaaagaa attgcaaaat
     5161 cattattaga
//
LOCUS       DANRDI010000174         5105 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.175, whole genome shotgun sequence.
ACCESSION   DANRDI010000174 DANRDI010000000
VERSION     DANRDI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.175"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            261..1385
                     /locus_tag="PZ377_002448"
     CDS             261..1385
                     /locus_tag="PZ377_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDM6359304.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            1485..2462
                     /gene="hrcA"
                     /locus_tag="PZ377_002449"
     CDS             1485..2462
                     /gene="hrcA"
                     /locus_tag="PZ377_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDM6359305.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            2494..3120
                     /gene="grpE"
                     /locus_tag="PZ377_002450"
     CDS             2494..3120
                     /gene="grpE"
                     /locus_tag="PZ377_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDM6359306.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            3189..5021
                     /gene="dnaK"
                     /locus_tag="PZ377_002451"
     CDS             3189..5021
                     /gene="dnaK"
                     /locus_tag="PZ377_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDM6359307.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
BASE COUNT         1989 a          723 c          924 g         1469 t
ORIGIN      
        1 tatgcatgag ttttactcat gtaattttat ttttaaggac atattacatc tggctaatgt
       61 gtaagagcca ctacataata aatcattagt ggttctttat tatttctatc tcactccctc
      121 taaacaagaa taaatattaa aatgaatcga tatattagac aatcattgat taaacgttaa
      181 agttaaaagt aagaataatt gcagatagtc caacaggata tagccgattg gataaaaagt
      241 ctgagaagcg gggcattaaa atgacggtac aaagtgcata tatacatatt ccattttgtg
      301 taagaatatg tacatattgt gatttcaata aatattttat acagaatcaa cctgtagatg
      361 agtacttaga tgcactaatc acagaaatgt ctacagcaaa atataggatc ttaaagacca
      421 tgtatgtagg tggcggcaca ccaacggccc tttctattaa tcagttggaa agattactta
      481 aagcaatacg tgatacgttt acaatcacag gcgagtatac atttgaagca aatcctgatg
      541 agttaactaa agagaaagtc caactattag agaaatatgg agtaaaaagg atttcaatgg
      601 gcgttcaaac attcaagccg gagttattgt ctgttttagg tagaacgcac aatactgaag
      661 atatttacac ttcggtgtta aatgctaaaa acacaggtat taaatcaatc agtttagatt
      721 taatgtatca tttaccgaaa cagacgattg aagattttga acaaagttta gatctagctt
      781 tagatatgga tattcaacat atttcgagtt acggcttaat acttgaacct aaaacccaat
      841 tttataatat gtatagaaaa ggcttgctca aacttcctaa tgaggattta ggtgctgaca
      901 tgtatcagtt gctgatgtct aagatagaac aatctccttt ccatcaatac gaaatatcta
      961 attttgcatt agatggccat gaatcagaac ataataaggt ttactggttt aatgaggaat
     1021 attatggatt tggagcaggt gcaagtggtt atgtagatgg tgtgcgttat acgaatatca
     1081 atccagtgaa tcattatatc aaagctataa ataaagaaag taaagcaatt ttagtatcaa
     1141 ataaaccttc tttgactgag agaatggaag aagaaatgtt tcttgggttg cgtttaaatg
     1201 aaggtgtgag tagtagtagg ttcaaaaaga agtttgacca atctattgaa agtgtctttg
     1261 gtcaaacaat aaataattta aaagagaagg aattaattgt agaaaagaac gatgtgattg
     1321 cacttacaaa tagagggaaa gtcataggta atgaggtttt tgaagctttc ctaataaatg
     1381 attaaaaaaa ttgaaatttc gagtctttaa cattgactta ctttgaccaa tttgataaat
     1441 tataattagc acttgagata agtgagtgct aatgaggtga aaacatgatt acagataggc
     1501 aattgagtat attaaacgca attgttgagg attatgttga ttttggacaa cccgttggtt
     1561 ctaaaacact aattgagcga cataacttga atgttagtcc tgctacaatt agaaatgaga
     1621 tgaaacagct tgaagattta aactatatcg agaagacaca tagttcttca gggcgttcgc
     1681 catcacaatt aggttttagg tattatgtca atcgtttact tgaacaaaca tctcatcaaa
     1741 aaacaaataa attaagacga ttaaatcaat tgttagttga gaatcaatat gatgtatcat
     1801 cagcattgac atattttgca gatgaattat caaatatatc tcaatataca actttagttg
     1861 ttcatcctaa tcataaacaa gatattatca ataatgtaca cttgattcgt gctaatccta
     1921 atttagttat aatggttatc gtattttcat caggtcatgt tgagcatgta catcttgctt
     1981 cagacatacc gtttaataat gacaaactaa atacaatatc aaattttgtt acaaacaaac
     2041 taactgagtt taatcagaat ttacaagatg atattgtctc ttttgttcaa tcagagcagg
     2101 aagaaatttt tatcaataaa ttgttaaata cgatgaataa tcatatttct aatcaaagta
     2161 atagcattta tatgggtgga aaggttaaac ttattgatgc attgaatgaa agtaatgtat
     2221 cttcaattca gcccattcta caatatatag aatcaaatag aattgcagaa ttattacaag
     2281 atatatcttc accaaatatt aatgtaaaaa ttggtaatga aattgatgac agcttaagtg
     2341 atatttcaat tgtaacaagt caatatcatt ttgatgaaac gttaaaaggt caaatcgctg
     2401 tgattggacc tacagctatg cattatcaaa atgtcattca attattaaat agaatttggt
     2461 aatgatggat tagaaagtat tggaggacag aaaatgacaa ataaagacga atcagttgaa
     2521 aaaaacactg aatcaacagt tgaagaaaca aacgtcaaac aaaatattga tgattcagtt
     2581 gaacaagctg aagaaagtaa aggtcattta caagatgaag caatagaaga aacgtctgac
     2641 gagaatgtta ttgaagaaat agatccaaaa gatcaaaaaa ttaatgaact tcaacaatta
     2701 gcagatgaaa acgaagagaa atatttaagg ctctacgctg agtttgaaaa ttataagcgt
     2761 agaattcaaa aagaaaatga aataaacaaa acatatcaag cacaacgtgt gttaacagat
     2821 attttaccag caatagacaa tatagaacgt gcacttcaaa ttgaaggtga tgatgagact
     2881 tttaaatctc ttcaaaaagg tgtacaaatg gtgcatgaaa gtttgattaa cgcactaaaa
     2941 gataatggtc ttgaagttat taaaactgaa ggtgaagcat ttgatccaaa tattcaccaa
     3001 gctgtagttc aagatgataa ccctgatttt gaatctggcg aaatcactca agaactacaa
     3061 aaaggataca agcttaaaga tagagtatta agaccatcaa tggtcaaagt aaaccaataa
     3121 cttaaatttg gcgaaaagac attgtttaaa attaaattaa tttaatgatt aaatggagga
     3181 attttattat gagtaaaatt attggtatag acttaggtac aacaaattca tgtgtaacag
     3241 tattagaagg cgatgagcca aaagtaattc aaaaccctga aggttcacgt acaacaccat
     3301 ctgttgtagc tttcaaaaat ggagaaactc aagttggtga agtagcaaaa cgtcaagcta
     3361 ttacaaaccc aaacactgtt caatctatta aacgtcatat gggtactgat tataaagtag
     3421 atattgaagg taaatcatac acaccacaag aaatctcagc tatgatttta caaaacttaa
     3481 aaaatacagc tgaaagctat ttaggtgaga aagttgacaa agctgtaatt acagtacctg
     3541 catactttaa cgatgctgaa cgtcaagcaa ctaaagatgc tggtaaaatt gctggtttag
     3601 aagttgagcg tatcattaat gaaccaacag ctgcagcatt agcatatggt ttagacaaaa
     3661 ctgataaaga tgaaaaagtt cttgtttttg acttaggtgg cggtacattt gacgtatcta
     3721 tcctagaatt aggtgacggt gtattcgaag tactatcaac agccggtgac aacaaacttg
     3781 gcggtgatga ttttgaccaa gtaattattg actacctagt tgcagaattc aaaaaagaaa
     3841 atggcgtaga cttatctcaa gataaaatgg cattacaacg tttgaaagat gctgctgaaa
     3901 aagctaaaaa agacttatca ggtgtatcac aaactcaaat ctcattacca tttatctcag
     3961 ctggtgaaaa cggtccatta cacttagaag taaacttaac tcgttctaaa tttgaagaat
     4021 tatcagattc attaattaga agaacaatgg aacctacacg ccaagcaatg aaagacgctg
     4081 gcttaacaaa ctcagatatc gatgaagtta tcttagttgg tggatcaact cgtattccag
     4141 cagtacaaga agctgtcaaa aaagaaatcg gtaaagagcc taacaaagga gtaaacccgg
     4201 acgaagtagt ggcaatggga gctgcaatcc aaggtggcgt tatcacaggt gacgttaaag
     4261 acgtagtatt attagacgta acaccactat ctttaggtat tgaaatttta ggtggacgta
     4321 tgaatacgtt aattgaacgt aacactacga ttcctacatc taaatctcaa atctattcaa
     4381 cagcagtaga taatcaacca tcagtagatg tacacgtatt acaaggtgaa cgtccaatgg
     4441 ctgcggataa taaaacactt ggtagattcc aattgactga tattccacca gctgaacgtg
     4501 gtaaacctca aattgaagta acgtttgata tcgataaaaa cggtattgta aatgtaactg
     4561 caaaagactt aggtacaaat aaagaacaaa gaattacaat tcaatcaagt tcttcattat
     4621 cagacgaaga aatcgaccgt atggtaaaag atgctgaagt taacgctgaa gcagataaaa
     4681 aacgtcgtga agaagtagac ttaagaaacg aagctgacag tctagtattc caagttgaaa
     4741 aaactttaac tgatttaggc gaaaatatcg gtgaagaaga taaaaaatct gctgaagaga
     4801 aaaaagacgc tcttaaaact gctttagaag gtcaagatat agaagatatt aaatctaaaa
     4861 aagaagaact tgaaaaagtg attcaagaat tatcagcaaa agtatatgag caagcggctc
     4921 aacagcaaca acaagcacaa ggtgctaatg ctggtcaaaa caatgatagt actgtagaag
     4981 atgctgaatt taaagaagta aaagacgacg acaaaaaata attaaacaat acaattagaa
     5041 tgttgtcatg atctgaaaag tcaaagtcaa tagaacattg gctttggctt tttatttgga
     5101 tagta
//
LOCUS       DANRDI010000175         5072 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.176, whole genome shotgun sequence.
ACCESSION   DANRDI010000175 DANRDI010000000
VERSION     DANRDI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.176"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1297
                     /gene="der"
                     /locus_tag="PZ377_002452"
     CDS             <1..1297
                     /gene="der"
                     /locus_tag="PZ377_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDM6359308.1"
                     /translation="VAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYSSGEWL
                     THDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEMVAQIL
                     YKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVVSHFGE
                     EEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEYSYDGQ
                     DYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDKRVAGY
                     AHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERTRLRTL
                     FPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEPPTFVV
                     FVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            1314..2312
                     /locus_tag="PZ377_002453"
     CDS             1314..2312
                     /locus_tag="PZ377_002453"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDM6359309.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            2483..2755
                     /locus_tag="PZ377_002454"
     CDS             2483..2755
                     /locus_tag="PZ377_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDM6359310.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            3186..3758
                     /locus_tag="PZ377_002455"
     CDS             3186..3758
                     /locus_tag="PZ377_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDM6359311.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            3761..4486
                     /locus_tag="PZ377_002456"
     CDS             3761..4486
                     /locus_tag="PZ377_002456"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDM6359312.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            4488..>5072
                     /locus_tag="PZ377_002457"
     CDS             4488..>5072
                     /locus_tag="PZ377_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDM6359313.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLK"
BASE COUNT         1834 a          683 c          988 g         1567 t
ORIGIN      
        1 agtagctatt gtaggtaggc ctaatgtagg taaatctaca atttttaata gaatagttgg
       61 agaacgtgtt tcgattgtgg aagacacgcc aggtgtaaca cgagatcgta tttattcttc
      121 aggtgaatgg ttaacacatg atttcaatat tattgataca ggtggtattg aaattggtga
      181 tgcaccattc caaacacaaa ttagagcgca ggcagaaatc gccatagatg aagcgtatgt
      241 tattattttt atggttaacg tgcgtgaagg attgacacaa agcgatgaaa tggtcgctca
      301 aattttatac aaatctaaaa aaccggtcgt attagcggtt aacaaagtag ataatatgga
      361 aatgcgtaca gacgtgtatg atttctattc attaggattt ggtgaaccgt atccgatatc
      421 agggtcacat ggtttaggtc ttggtgactt gttagatgca gttgtttctc attttggtga
      481 agaggaagaa gatccttatg atgaagatac aattcgacta tccattattg gacgaccaaa
      541 cgtaggtaaa tcaagtttag taaatgctat tttaggtgaa gatcgcgtta tcgtttctaa
      601 tgttgcaggg acaacgagag acgctattga tacagagtat agttatgatg gacaagatta
      661 tgttttaatc gatactgctg gtatgcgtaa aaaaggaaaa gtatatgaat caactgagaa
      721 atattcagta ttaagagctt taaaagcgat tgaacgttca aatgttgttt tagtggtcat
      781 agatgcagaa caaggcatca ttgaacaaga taaacgtgtt gcaggatatg cacatgaaca
      841 aggtaaagca gtcgtgattg tcgtaaataa atgggatact gtggaaaaag atagtaaaac
      901 gatgaagaaa tttgaagatg aagtacgtaa agaattccaa tttttagatt atgcacaaat
      961 tgcttttgtg tctgctaaag aacgcacaag attacgtaca ttattccctt acatcaatga
     1021 agcaagtgaa aaccataaaa aacgtgttca aagttcaact ttaaatgaag ttgttactgg
     1081 tgcaatttcc atgaacccta caccaacaga caaaggtaga cgtttgaatg tcttttatgc
     1141 aacacaagtt gctatagaac caccgacatt tgttgtattt gttaatgatg tagaattaat
     1201 gcatttttct tataaacgct atttagagaa tcaaatccgt gccgcttttg gttttgaagg
     1261 tacaccaatt catattatag ctcgaaagag aaattaacga ttgggggata acaatgacta
     1321 aaattaccgt ttttggtatg ggaagttttg ggacagccct tgccaatgtt cttgcagaaa
     1381 atggacatga tgttttgatg tggggtaaaa atcaagatgc tgttgatgaa ttaaatacat
     1441 gtcatacaaa taaaaagtat ttaaaatacg cgaaattaga tgttaacatc atcgctactt
     1501 cagatatgac caaagcaatt caatttgcag atatttactt aatggcttta cctactaaag
     1561 caatgcgaga agttgcttct caaattaatg ataagctgac ctctaaaaag acttttatac
     1621 atgttgctaa aggtattgaa aatgggacgt ttaaacgtgt gtcagaaatg attgaagatt
     1681 ctatttcacc tgaatataat gcaggtattg gcgtgttgtc agggccaagt catgcggaag
     1741 aagttgtagt caagcaacca actacagttg ctgcttcatc aaaagataaa agtgtaagta
     1801 aattaacgca agatttattt atgaatgatt atttgcgtgt gtacacgaat gatgacttga
     1861 ttggtgttga acttggtggt gcattgaaaa atatcatcgc agtagcaagt ggtatcgtag
     1921 ctggaattgg ctacggtgat aatgcaaaag ctgcattaat gactcgtggc ttagcggaaa
     1981 ttagtagatt aggtgaaaag ttaggtgccg atcctatgac atttctaggt ttaggtggta
     2041 tcggtgactt aatcgttact tgcacatcaa cacattctcg gaatttcaca ttaggatata
     2101 aacttggaca aggtgaatca atggatcaag cattatctga aatgaatatg gttgttgaag
     2161 gtatttatac aactaaatca gtttatcatt tagctaaaga aaaaaatgtg gatatgccaa
     2221 ttacaaatgc attatataga gtattatttg aaaatatctc agtaaaagaa tgcgtaaaag
     2281 atttaatgga gcgcgataaa aaatctgaat aaaatgtgta ttattacaca tatacggcta
     2341 ataattacgt aaaatgaata gaaatggctt aaattcaacg tttttgccac ataaatgatt
     2401 gcgatttatg taatgcttgt gttaaagttt taccataatg atataaacat atcattatca
     2461 aacctcagga ggtgaatgtc taatgaacaa aacagattta atcaatgcag ttgcagagca
     2521 agctgattta actaaaaaag aagctggttc agcagtagat gctgtattcg aatcaatcca
     2581 aaactcactt gctaaaggtg aaaaagtaca attaattggt ttcggtaact ttgaggtacg
     2641 tgaacgtgct gcacgtaaag gtcgtaaccc tcaaactggt aaagaaattg atatcccagc
     2701 aagtaaagtt ccagcattca aagctggtaa agcattaaaa gatgctgtaa aataatttta
     2761 cttaaaaagc cctgaataag ggctttttat tttgctttta atacttacaa cctgtacata
     2821 aattgtaatg ttcttctaag tttttaatct ttggaacatt cattgtttaa gcgttcaaaa
     2881 tattacatga cataatagtt ttgataaaaa tagcaactac aaaatataca tatgaatatt
     2941 gagattaatt gttagcgttg aatttactta aaaggtaacc atgtctacta tagtatttta
     3001 cgttatttaa aaagatgaat aatgtaaatg aagtaaaggt tattatgaga attacaaaag
     3061 ctacataaat tactgttagt ttaaattgaa atttaaaaat gataagtaat cttaatgctt
     3121 caaaagattt taatttctta catataatgt taatatgaat agtgataata tcgtgaggtg
     3181 tagacatgga aacaactgtt agcaaattgg aaagacaaat agaagaaaga ttaaagggcg
     3241 tatcagaata tgaatccatt aatattaatc atcggttagg taagttactt gatttatatg
     3301 atattccaga tgttgccaaa gtggcatgcc tcacaattga cacatcaatg agacaccttg
     3361 atgacattac atataatcat ttgtctaaac attccatttt gattggagat ttaattagtg
     3421 cacattttta tacgctacta gcagaaatca atgatttatc atttcaaaat gaaattagta
     3481 aagcaattgt tgaaattaat gaattgaaat cttcattaca tcatcaagct ttaaatgatt
     3541 atgaaatttc tcaagcaata gtaaaaattg aaacactttt cccatatatt acgttatctc
     3601 atttcggtat caatattgat gagtcggaaa tatataatta tttatttgaa gatatgtctg
     3661 attactatcc atcatacttc aagaaatata atcaaagtga agtaaagcat tacttacacg
     3721 atatccaaaa aagttattta aaaagtagag gtaattaagc atggctgaca ataaagcaaa
     3781 taaagagcaa gtacatcgcg tttttcaaaa tatttcaaaa aaatatgata gattaaataa
     3841 tattattagt tttgagcagc ataaagtatg gagaaaacgc gtcatgaaag acatgggtgt
     3901 tagaaaaggg acgaaagcat tagatgtttg ttgtggtact ggtgattgga caatcgcatt
     3961 aagcaaagct gtaggaccaa caggtgaagt tactggtatt gactttagtg agaatatgtt
     4021 agaagtagga aaagaaaaaa ctgcttcaat ggaaaatgtt aaacttgttc atggtgatgc
     4081 aatggaattg ccgtttgaag acaattcttt tgattatgta acaattgggt ttggattaag
     4141 aaatgtgcca gactatttag tcgcgttaaa agaaatgaat agagtactta aaccaggtgg
     4201 tatggtggta tgtcttgaaa cgagccaacc aactttgcca gtctttaaac aaatgtatgc
     4261 actatacttt aaatttgtta tgcctatttt tggaaaatta tttgcaaaat caaaagaaga
     4321 atatgaatgg ttacagcaat ctacgtttaa ttttcctgga aaagaagagt tgaagcgcat
     4381 gtttgaagaa gcaggtttca taaatgtaag agtacgtagt tttacagggg gcgttgctgc
     4441 aatgcacctt ggctataaag aaaaagataa taccaaaggt gattaacgtg gcaaagttaa
     4501 acatgaacaa tgaaattaag aaagtggaac aacgacttga aaaggcaata aaaagtaaag
     4561 attctgtatt agaacaggca tcattacact tattgtcttc tggtggtaaa agagtacgcc
     4621 cagcatttgt tattctgagt agccaatttg gcaaagatga acaaacgtct gaacaaacgt
     4681 atcaagttgc agtcgcatta gagttaattc atatggcaac acttgttcat gatgacgtta
     4741 ttgataaaag cgacaagcgt cgaggcaagt taaccatatc aaagaaatgg gatcagacaa
     4801 ctgctatttt aactgggaat tttttattgg cattaggact tgaacactta atggccgtta
     4861 aagataatcg tgtacatcaa ttgatatctg aatctatcgt tgatgtttgt agaggggaac
     4921 ttttccaatt tcaagaccaa tttaacagtc aacagacaat tattaattat ttacgacgta
     4981 tcaatcgcaa aacagcactg ttaattcaaa tatcaactga agttggtgca attacttctc
     5041 aatctgataa agagactgta cgaaaattga aa
//
LOCUS       DANRDI010000176         5051 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.177, whole genome shotgun sequence.
ACCESSION   DANRDI010000176 DANRDI010000000
VERSION     DANRDI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.177"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..598
                     /locus_tag="PZ377_002458"
     CDS             <1..598
                     /locus_tag="PZ377_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDM6359314.1"
                     /translation="KPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDFFTSQTG
                     ETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFGIMHEAK
                     DKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRAINVKKG
                     EFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            782..2134
                     /locus_tag="PZ377_002459"
     CDS             782..2134
                     /locus_tag="PZ377_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDM6359315.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(2216..2635)
                     /locus_tag="PZ377_002460"
     CDS             complement(2216..2635)
                     /locus_tag="PZ377_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDM6359316.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(2918..3937)
                     /locus_tag="PZ377_002461"
     CDS             complement(2918..3937)
                     /locus_tag="PZ377_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDM6359317.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(4011..>5051)
                     /locus_tag="PZ377_002462"
     CDS             complement(4011..>5051)
                     /locus_tag="PZ377_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDM6359318.1"
                     /translation="LVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGEYIDPELIKKFE
                     KETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHLLLPIDHNKVPN
                     IKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKSLYNPKFKNQIL
                     LVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGDEITMMLQQNE
                     GNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLD
                     AKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVYKDLGPEVLSEY
                     NENFLNFKMSLK"
BASE COUNT         1682 a          854 c          703 g         1812 t
ORIGIN      
        1 taaacctaaa gatttcaaag cgttcaacgt agaaggctta gacgcacgaa tggaagcttt
       61 aaacgaatac atacgaccac aactccgtga attaggagaa tattttagtg acttctttac
      121 aagtcaaact ggtgaaacat tttatcctca cgtagcaaag catgctagaa gaagtgtgaa
      181 tcctcctaaa gatacatggg ttgcttttgc aacaaacaaa agaggctata aaatgttacc
      241 tcatttccaa attggtatgt ttgaagatca actgtttgtt atgtttggaa tcatgcatga
      301 agcaaaagat aaagcaacac gtgcaaaagt ttttgaaaga aaatttaaag ctattcaaca
      361 attacctgat gattatcgtg tttgcttaga tcatatgaaa cctgataaac catttattaa
      421 agatttaacg gatgatgatt taatagaagc gatacaaaga gccatcaatg tgaaaaaagg
      481 tgaattcttt atagcgcgtg caatcacacc acaagataaa agattaaaaa gtgacaaagc
      541 atttattgca tttttagaag aaaccttcga tcagttctta ccattttatt ctgcataaat
      601 aactttgttt aaataataga gcacttaatc acatccatga tttcgtgctc ttttttctta
      661 atattaaatc gaacgttcaa cataataatt catactttta aaaaaattaa aataaattta
      721 ggttgaccta aacattttat taggttatta tattgtccat aagaagtaga ggtgagtcaa
      781 aatgaataat aaacgacatt caacaaatga acaattaagt ttagacgaaa taaacaatac
      841 aattaaattc gatcatcgca gttcaaataa acagaaattt ttatcatttc ttggacctgg
      901 gttattagtc gctgttggtt acatggatcc cggaaactgg ataacatcaa tgcaaggtgg
      961 cgcccaatat ggctatactt tgctattcgt aattcttatt tcaagtttat cagcaatgtt
     1021 acttcaaagc atgacagtga gattaggaat agcaacaggt atggacttag ctcaaatgac
     1081 acgacattat ttatcaagac ctattgctat aatcttttgg atcattgcag aactagcaat
     1141 tatcgctaca gatattgctg aggttattgg tagtgctatt gctcttaatc tcctatttaa
     1201 cataccttta atcgtcggtg cactaataac tgtacttgat gtatttttac tactttttat
     1261 aatgaaatat ggttttagaa aaattgaagc tattgttggt acattaattt tcacagtgtt
     1321 attcatcttt atatttgaag tctatatttc atcaccacag ttgaatgctg tgttaaatgg
     1381 atttatacca catagtgaaa tcattacaaa taacggcatt ctctatattg cattaggtat
     1441 tattggcgct acaattatgc ctcataattt gtacttacat tcatcaattg tacaatctag
     1501 aacatactca agacataaca atgaagaaaa agcgcaagcg attaaatttg ctacgataga
     1561 ttcgaacatt cagttatcaa tcgcatttgt agtcaattgc ttattattag tgttaggagc
     1621 atcactattt ttcaactcaa atgctgacga tttaggtggt ttctatgatt tatatcacgc
     1681 cttaaaaact gaacctgtac taggtgcaac aatgggtgca atcatgagta cattatttgc
     1741 agttgcatta ttagcgtcag gtcaaaattc aacgattact ggtactttag caggacaaat
     1801 tgtaatggaa ggatttttaa gattacacat accaaattgg ttaagacgtt taattacacg
     1861 ttctcttgct gtcattcctg ttatcgtatg cttaatcatt tttaaaggta atgcagctaa
     1921 aattgagcaa ctacttgtat tctctcaagt atttttaagt attgctctgc cattctgttt
     1981 aataccatta caattggcta ctagtaataa agacttgatg ggacctttct ataataaaac
     2041 atgggttaat atcatttcat ggacacttat tattattcta agtattttaa atgtctattt
     2101 aatcgttcaa acattccaag aactacaaag ttaaaaataa aaccattaaa atgttggttg
     2161 tatctaataa ttaacaactt aacattttaa tggtttttct tttgctatga acatattaat
     2221 gataaatata ctcatcgtcc ttatgtgttg tttcttttga ttctttttta gaaatcaaca
     2281 taatcatcac gataacccat aagacaattg caattaaatt aagagccatc aaacttacaa
     2341 ttatcgcgat aacaaaaagt ataatcgata attttctacg tcctttaata caaattgtcg
     2401 caaatatacc ataaagtgtt gaaattaata aaataattgc taatgcaccc attgttgtct
     2461 gaagcatatc taaagaaacc ttgccaccgt tctgagataa ttgcttcgct aattcttcac
     2521 gttgtgatgc attaccactt tttaaagaaa aataggatag ccccataact aataaataaa
     2581 taatactaat actatttgca atccaagcca tgattaattc aggttttcta ttcatcttaa
     2641 aatcctttct ttgtttaata ttttaataag tatactacga gcctatagat atacgcacat
     2701 taaattactt gtatattaac atatttttca tctaattttt ataataaaca tctgaattca
     2761 gattgatgat aatttaggtg tatagtaatg agaaatcgtc atttaaaatt atgacaccta
     2821 aacactttaa ttaaacgtta tcgataatta acctcatata aaaacccaga ttaataagca
     2881 aaagacctct gcttactaat ctgggtatca tcaatattta ttcttgctct tttttgtcct
     2941 taacttcact tgaattgcgt tcttcagaaa tatcattgtt gtaaatttga cgtcgctctt
     3001 caaaattcat tcgacgttga ctaactgcgc taggttgatt tttcctacgt tctttcattc
     3061 gttggttata agctttacgt ttttcttttt tcttagcttt aatttcagct ttttctcgct
     3121 tttgttgtgc tttcaattct tttggatcaa cttcaggttc tgcataataa ccatcatctt
     3181 caatacctag ttcttcagta cttttttgtt tgtatttagc ctgtctggat aatactgatg
     3241 gttcttcctc agtgcgctca tcatgattat attcactttc aactgcacgt ttaggaaatt
     3301 gatcatattt atctctttcc tgttcttgat catatacagt atttgattta tccaattctt
     3361 tttcatattt aggcacatca taataatcat ctttcggttg ggggcgctca tacacaggtt
     3421 cacgataatc ataaatcggt ccgttatact cttcataata ttgtggcgca accatttcaa
     3481 ctttatcttt tcttataaat agcatcatcg caataataaa gaataatatc ggaatgatta
     3541 aagtaacgat aagtaaaact aaaggaatcg ttacaattgc tgatattaag aataagaaac
     3601 ctgacaagat tcttatattc atagatatga gagctaaaaa tgaaatcaac aaacaaacaa
     3661 taaagtaaac gataatagcc catataccat tttgtaacca tattacaaat tgtgttgtat
     3721 tatagccatt tccagctaat aattgctgga taaatgcatt attgtttaat gtattttcaa
     3781 gattagcaat cgatgtgtta ttactgaatg aaacaagtgc tataaacatc gtaatgacag
     3841 taagcactaa taacatcacc caacataacc aacctagaac tttttcagtt aatctactta
     3901 ctggacgttt aatttgagta aattgttctc cagtcattcg ttacaactcc ttatagtact
     3961 tatcccgtta ttataactaa atatacagta aataactact atttatgatt ttattttaat
     4021 gacattttga aattcaaaaa gttttcattg tattcactta aaacttcagg tcctaaatct
     4081 ttataaactt caaggcgttc ttgctctttc ttagtcggat aaaaacgatg gtcgtcttta
     4141 atctctttag gcaacaattg tcgagcagcc ttgtttggcg ttgcatagcc tacgaattct
     4201 gtattttgct tgttattttt agcatctaat aaaaaattca taaatttata tgcaccctct
     4261 ttattttgtg ccgtttttgg aattaccata ttgtcgaacc ataaattcga tccttcttta
     4321 ggaataacat aattatattt atccccttct tgcactagag gtgctgcaac accactccaa
     4381 acaaccgcta tgttaccttc attttgttga agcatcatgg taatttcatc acctacgaca
     4441 cctcttactt gtggtgctag tttggttaaa tctcgctctg cttcttttaa atggtgcgaa
     4501 ttacggtcat taagattata cccaagttta ttcaaactca tgcctataat ctctctagca
     4561 ccgtcaacta gtaaaatttg gtttttaaat ttaggattat acaatgactt ccaactatca
     4621 aatgattcat ttggatactt ttctttatta tataaaatac ctacagttcc aaagaaataa
     4681 ggtaaagaat atttattgcc tctatcaaat gacatattca tataatctga atctaaattt
     4741 ttaatattag gtaccttatt atgatctatt ggtaacaata aatgatctct tttcaatttt
     4801 tgaactgtat attcactagg aaaagcaaca tcataatgtg taccgccatt gcgaattttg
     4861 gcttccatcg cttcatttga atcgaaagtt tcataaacga cttgaatgcc agtttctttt
     4921 tcaaatttct taattaactc tggatcaata tattcgcccc aattgtatac gtaaattttt
     4981 tgatttgtat gcacttgttc tttagattta aaccaatgac ttaaagtaag acaaagcata
     5041 cccacaacta a
//
LOCUS       DANRDI010000177         4974 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.178, whole genome shotgun sequence.
ACCESSION   DANRDI010000177 DANRDI010000000
VERSION     DANRDI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.178"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..385)
                     /locus_tag="PZ377_002463"
     CDS             complement(<1..385)
                     /locus_tag="PZ377_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDM6359319.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTP"
     gene            complement(676..1371)
                     /locus_tag="PZ377_002464"
     CDS             complement(676..1371)
                     /locus_tag="PZ377_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDM6359320.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            complement(1657..2337)
                     /locus_tag="PZ377_002465"
     CDS             complement(1657..2337)
                     /locus_tag="PZ377_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDM6359321.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            2809..3654
                     /locus_tag="PZ377_002466"
     CDS             2809..3654
                     /locus_tag="PZ377_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDM6359322.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            3673..4032
                     /locus_tag="PZ377_002467"
     CDS             3673..4032
                     /locus_tag="PZ377_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDM6359323.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            4556..4708
                     /locus_tag="PZ377_002468"
     CDS             4556..4708
                     /locus_tag="PZ377_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359324.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
BASE COUNT         1579 a          816 c          750 g         1829 t
ORIGIN      
        1 tcggcgttgt ggattcggtt gtcgtttgtg attgttcttg tttaggcgct ggcgttgctg
       61 atatattaag cgttttctgc tcttcttgtt taggttgtga tattttttct attttggaag
      121 ctgaggtttt ttcctcatta gtatttggtg ccttttcgag tttaggcgtg cgttcttgtc
      181 ttgtgttagc tgcttgtgtt gtcgctgaat ttgcacctgc tgttatgttt atcattgcta
      241 atcgctctgc tttaagcgtt ggtactttgt caactttagt tgattgtatt ttttctgctt
      301 tgaccgattg cgtcgttact gtaattgcgc ctgttgttaa aagccctagt gctaaactgg
      361 ttttagcaat tgttctcatt ttcataattg tatgctccaa tctatattat attcgattgt
      421 ctttttacgt aatttgaatc atacaacatc attatagatg gcgttctaag ataatcacat
      481 taaacccctt ttaacaatta ttgaagtatt attaagtaat ttaagcaaaa aataatgagt
      541 gagtatgaga ttaatatagc gtttctatgt gcctttgaaa taatttttaa gcattaaaaa
      601 gaagttaagc aacgtttgat cgtcacttaa cctctctatt tcaatttcaa cttatttcgt
      661 catcaagtat atgtgttatg cttttataac tttgatttca attctatcaa tatctgtgac
      721 attgataaca tcggacatac ggtcttcttg taacttttta tccaattcaa atgtatactt
      781 tccatagtat ttctttttga ctgtaatttt tcctgtactc atttcaccgt aaagaccata
      841 attatcaata aggtattttc ttaatttaaa atcaatctct ttcaatgaca tcgcttcttt
      901 atctatttta aatgggaaaa agtcataatc atattcacca gtatgatctt ctttaataac
      961 tcttgcttct gctattaggt cgacagcttt atcgtttgca ctcgtgatac ccccaataga
     1021 gtactttgca ccttcaaatc tcttatcctc attaacgtaa aatatattaa gattacgatg
     1081 tacacccgta tgataatgtt gcttatcttt gccaattaaa gcaatattat taacagaatt
     1141 accatctatg atattcataa atttaatact tggttgaatg aaactgatat aacctgtcac
     1201 atttttatat tcaatactag gttgattata ataagctttt aattttttgc tattttcact
     1261 tattacaata ggtttctttt cggcatgaac tggtttttcc gttgtagtgt ttacacctgt
     1321 tgctaatatt cctaataaca aacttatttt tgcaatattt ttcattttca tagttgtatg
     1381 ctccaatcta ttataattag attgttttat tacgtaattt gaatcataca cccatattat
     1441 aggagctgta ttcggatatt cacattaacc tgtttttaac tattcataaa atatgattaa
     1501 gctatttaag caaaagataa tgcgtggtgc gactgtttgt gtgagttttg taacttttcc
     1561 cctcaaaaaa agaagctaag caacagtacg tcgcttaact tccatatatt tccataacaa
     1621 gcactattca acatagcgct ttgttgttag aaagtattat ttcatttcta ctagaatttt
     1681 attaatctta gtgccgtcga ttgactcacc catacgttct ttttcaagtt tttgacttaa
     1741 atcgattgta tgtgttgtgc catcattcat tgtaattgta atttgacctt gtttaagacc
     1801 atttgaatac aagccgtgtt gtttaatcgc acgttctctt aatctataat caagttcttt
     1861 taatgagatg tcttcttttg aaatgtagta aaaatcttta agtacatctt cattatcttt
     1921 cttgatttct aaaattggcg tattgatata atcaatatac tgcacactat ttgattttgt
     1981 aacgccacca attgagtaat ttgttgcttg tctactgtca ccttctctaa ggataaacac
     2041 atctatattt gagttttctc cgtctttaaa tttatcttta tcagatccaa gtaaagtgat
     2101 tcgagaatgc tgttgcccta ctgtgacttc taaatagtgt ttaccctcat cagtatattt
     2161 aaatccagtc acatttttac gctctaaaat tggtttatta taatagtgtt ttaactctga
     2221 ttcactttgt tgtttaactt ctgctttcgc ttgtactgat tgtacattcg atgtaattac
     2281 acctgttgct aacattccca atgctaaact tgcttttgct atcgctttaa atttcataat
     2341 ttttagcccc aatctattta aatttgattt ttcaattacg taatatgaac aatctaccta
     2401 cattatattg aggcgctatc ccctttcaaa ttaaacctca tttaactaaa cttgaatcat
     2461 tgttaaggta attaataaaa tgctaactgt tattaatcaa ttcttatccg tcgagactct
     2521 tttttataca aatcacactt tataccattt ttattttaaa aactgtaaaa ctttcagaca
     2581 caaaacagac atcatattgt cgtagtacct gattaaaatt ttcaatgata ttagtgatac
     2641 acatcaacta aaacgcgtca ttacggcatt ttcaacatta ataatattta cgtatttact
     2701 atacacgtcg acaataaata cagattagaa ttttttataa aatcaaatgt tttgaaaacc
     2761 atcaaataag tattatgtta tgtatataaa gattaggaga tgaagtgaat gaatattatg
     2821 ttaacaggtg ctacaggtca tttaggcaca catattacaa atcaagccat tgcaaatcac
     2881 atagatcatt ttcacattgg tgttagaaat gttgagaaag ttccagaaga ttggcgcgga
     2941 aaagttcctg ttcgacaatt agattatttt aatcaagaaa gcatggtaga agcatttaaa
     3001 ggaatggata ccgttgtgtt tattccaagt attatccatc catcattcaa acgtattcct
     3061 gaagtggaaa atttagtata tgcggcaaag cagagcggcg ttgctcatat cattttcata
     3121 ggttattacg cagatcagca taataatcca ttccatatga gtccttattt tggttatgca
     3181 gcacgtctat tggcaacaag tggcattgac tatacgtatg taagaatggc aatgtacatg
     3241 gatccactta aaccatattt accagaattg atgaatatgc ataaactgat ttatccagct
     3301 ggcgatggtc gtattaatta tattactaga aatgatattg ctagaggtgt cattgctatt
     3361 attaaaaatc cagatacttg gggcaaacgc tacttattat caggctacag ttatgatatg
     3421 aaagaacttg ctgcaatttt atctgaggca tcaggcacag aaattaaata tgagcccgtt
     3481 tcattagaga catttgcaga aatgtatgat gaacctaaag gctttggtgc attattggca
     3541 tcaatgtacg acgcaggagc aagaggacta ttagaccaag aatccaatga tttccaacaa
     3601 ttagtcaatg atcaaccaca gacactgcaa tcatttttac aagaaaatat ttaataataa
     3661 aggagcgtta tagtgaatat catctcaaca attttaatca tatttgtggc attagagttt
     3721 ttctatatta tgtaccttga aacgattgct acaacttcca aaaagactag cgagacattt
     3781 aatataagcg tcgataaatt gaaagacaaa aatattaacc tacttttgaa gaaccaaggc
     3841 gtatataacg gtttaatcgg agttttgcta atatacggtt tgtttatcag cagtaatcca
     3901 aaagaaatat gcgcagctat tttagtgtat atcattggcg ttgctattta tggtggcctt
     3961 tcaagcaata ttagtatctt tttcaaacaa ggcacattgc cagtattggc actcatatca
     4021 atgctttggt aagtattggt gtttgggggg gtggagatgt agtcggaggt ttggaggatt
     4081 tgagcgaatt gtgtgtggac tttagactca gagtatttca tcctaattat ttcaagcaga
     4141 ggtgacagta gcgttgcctc tgtttcctta tagaaaaatt atttaatgaa gaaaacccat
     4201 atctgattta attttcagct gataaatact ccatatatta gaatggctac tttatctatt
     4261 gcatcaatcc tttaaaacaa aaaacccatg atttcgaaat tcccgtatga tggggttcct
     4321 actctcatgg atcagttaaa taaatattat cactatcagt ttattatttc aatattatta
     4381 acaatatatg tagtcgtaaa aggaaagagg acatgagaac ttcggtgttg attggcatta
     4441 cataacgctt ccaaacatat tatttggtaa caataagaaa ctatttacac aatatatttt
     4501 gtatagtaaa attattttat aatatttaaa tccaattgca caaggagtga ttatcatggt
     4561 accagaagaa aaaggttcta ttactttgtc aaaagaagca gctatcatat ttgcaatcgc
     4621 aaaattcaaa ccatttaaga acagaattaa aaataaccca caaaaaacaa atccatttct
     4681 taaattacat gaaaacaaaa aatcttaatc actttattta tagcatttct aatctcagaa
     4741 atgctataaa ggacaacgcc ctctttcata actatataag tcaagaaacc aagacatttc
     4801 aaacatcttt aaccccttca atagcacatt ccctaaaaca taaagcaata acaatgaacg
     4861 atcaaaattc cttacgtact tatgatgtgt cagtgaatcg tctacactta attgaacaaa
     4921 ttctatgaga atagatattg ttaaattaag aaagtagtca attttattat gaca
//
LOCUS       DANRDI010000178         4931 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.179, whole genome shotgun sequence.
ACCESSION   DANRDI010000178 DANRDI010000000
VERSION     DANRDI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.179"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            24..2393
                     /locus_tag="PZ377_002469"
     CDS             24..2393
                     /locus_tag="PZ377_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDM6359325.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            2398..3111
                     /locus_tag="PZ377_002470"
     CDS             2398..3111
                     /locus_tag="PZ377_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDM6359326.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            3142..4407
                     /locus_tag="PZ377_002471"
     CDS             3142..4407
                     /locus_tag="PZ377_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDM6359327.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            4407..>4931
                     /locus_tag="PZ377_002472"
     CDS             4407..>4931
                     /locus_tag="PZ377_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDM6359328.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGS"
BASE COUNT         1885 a          693 c          894 g         1459 t
ORIGIN      
        1 ggcaacataa ggaggtgcgt caattggcac aagcaaaaaa gaaatcgaca gctaagaaaa
       61 aaacaacatc aaaaaaaaga acaaattcga ggaaaaagaa gaatgataat ccgatacgtt
      121 atgtcatagc tattttagta gttgtattaa tggtgttggg tgttttccaa ttaggaataa
      181 taggtcgtct aattgacagc ttctttaatt atttatttgg gtacagtaga tatttaacat
      241 atattttagt actcttagca actggtttta ttacatactc taaacgtatt cctaaaacta
      301 gacgaacggc tggttcgatt gtattgcaaa ttgcattgct atttgtatca cagttagttt
      361 ttcattttaa tagtggtatc aaagctgaaa gagaacctgt actttcttat gtgtatcagt
      421 cataccaaca cagtcatttc ccaaattttg gtggcggtgt attaggcttt tatttattag
      481 agttaagcgt acctttaatt tcattatttg gtgtatgtat tattactatt ttattattat
      541 gctcaagtgt tattttatta acaaaccatc aacatcgtga agttgcaaaa gttgcactgg
      601 aaaatataaa agcttggttt ggttcattta atgaaaaaat gtcggaaaga aaccaagaaa
      661 aacaattgaa gcgtgaagaa aaagcaagac ttaaagaaga acaaaaggca cgtcaaaatg
      721 aacagccaca aataaaagat gtgagtgatt ttacggaagt gcctcaagaa agagatattc
      781 caatttatgg gcatactgaa aatgaaagta aaagccagag tcaaccaagt cgaaaaaaac
      841 gagtgtttga tgcagagaat agttcgaata acatcgtaaa tcatcatcaa gcagatcagc
      901 aagaacaatt aacagaacaa actcataaca gtgttgaaag tgaaaacact attgaagaag
      961 ctggtgaagt tacgaatgta tcgtatgttg ttccaccgtt aactttactt aatcaacctg
     1021 caaaacaaaa agcaacatct aaagctgaag tgcaacgtaa aggacaagta ctagagaata
     1081 cattaaaaga ttttggggta aatgcaaaag tgacacaaat taaaattggt cctgcagtaa
     1141 ctcaatatga aattcaacca gctcaagggg ttaaagtgag taaaattgta aacttgcata
     1201 atgatattgc attagcttta gcagcaaaag atgttagaat cgaagcgcca atacctggtc
     1261 gttctgcagt aggtattgaa gtgccaaatg agaaaatttc attagtttca ctaaaagaag
     1321 ttttagatga aaaattcccg tctaataata aactagaagt tggattagga agagatatat
     1381 caggtgatcc aattactgtt ccactaaatg aaatgccaca cttattggtg gcaggatcga
     1441 cgggtagtgg taaatctgtt tgtataaatg gtattattac aagtatttta ttaaatgcta
     1501 agccgcatga agttaaactt atgttaatcg atccgaaaat ggttgaacta aatgtttata
     1561 acggaattcc acacttatta attccggttg ttacaaatcc tcataaagct gctcaagctt
     1621 tagaaaaaat tgtagctgag atggaaagac gttatgattt attccaacat tcatcaacta
     1681 gaaacattaa aggttataac gaattaatcc gtaagcaaaa tcaagaatta gatgagaagc
     1741 aaccagaatt accttatatc gttgttattg tagatgagct tgcagattta atgatggtag
     1801 ctggtaaaga agttgaaaat gcgattcaac gtattacaca aatggcacgt gcagcaggta
     1861 tacatttaat tgtagcgaca caaagacctt ctgtggatgt aattacaggt atcattaaaa
     1921 ataatattcc atctagaata gcttttgctg tgagttctca aacagattca agaactatta
     1981 ttggtactgg cggcgcagaa aagttacttg gtaaaggtga catgttatac gttggaaatg
     2041 gtgactcatc acaaacacgt attcaagggg cgtttttaag tgaccaagag gtgcaagatg
     2101 ttgtaaatta tgtagtagaa caacaacagg caaattatgt aaaagaaatg gaaccagatg
     2161 caccagtgga taaatcggaa atgaaaagtg aagatgcttt atatgatgaa gcgtatttgt
     2221 ttgttgttga acaacaaaag gcaagtacat cattgttaca acgccaattt agaattggtt
     2281 ataatagagc atctaggttg atggatgatt tagaacgcaa tcaggtaatc ggtccacaaa
     2341 aaggaagcaa gcctagacaa gttttaatag atcttaataa tgacgaggtg taaaaaaatg
     2401 tcagaaatga atgcggtata taacgttaaa caatacattt taaatttgat taagcaaaat
     2461 aaattggaat atggtgacca acttccaagt aatttatcaa ttgccagaga attaaatgta
     2521 aaaaccgacg atgtttatga agcaattcag gcattgatta ctgaacaagt cattaaagat
     2581 aattttgaag agggcacaag tgttaagtca ctgccccctt tcttttatcc attgaatgaa
     2641 cttataagta ttgggcaaat gattaaaaat gcaggatttg aatgcggaac tgaatactta
     2701 aattttgatg agcaaccagc aactatgtta gatgcaaatt tgttgagcgt tgaagaagga
     2761 tatccagtaa ccattataga acgattacga actgccgatg gagaaccggt cgtctattgt
     2821 ttagataaaa ttgctaaaaa agaattaaca tgtacagagt atcaaatgag caatggatcg
     2881 attctaagtg cgataaaaga acaaagtaat cataatattt gttatgctga tacagaaatt
     2941 gaagcggtaa attatgaacc tcgaatatcc gaagtactga atgcttcgcc acacgaaggt
     3001 ttgattttat taaaaattac gcactataat gaatcagatg aacctatttt gtattcatta
     3061 aattatatga aaaatagctt agttcaattt aaaatcacta gaaaaatata gatgtaagaa
     3121 gatatagagg aggctatata tttgagtaga caatctcaac caaatataca tatcaaagtt
     3181 tcaccaacaa ctaaatttaa aacaactact atagttttta aatttatggc acctttagaa
     3241 tatgacacaa taacagctag atcattatta agcaaattat tagttcgagc aactaagaaa
     3301 tggccaaccg ataagtcgtt taataatcac ttagccgatt tatatggtgc gtatgtgaat
     3361 agtacaattt caaaattcaa agatcagcat gtcattacat tttcattaga aattgttaat
     3421 gaacgttatt taagaaacgg tgaatcatta tttaatcaag gattagattt attacaagaa
     3481 atcatttgga atccattaat tgaaaataaa gcattcaatg ataattttgt taaccaagag
     3541 aaaacattat tagccaaaaa aatagaagca atggtagata ataaagcaca atattcgttt
     3601 ttaaaattac tcgaccatat gtttgaaaat gaagcatata aatacttatc tacaggacaa
     3661 ctagaacaaa tcccacatat tactgctgaa acactatatc atacatatca atcaatgatt
     3721 aataatgatc aatgttctgt ttatgttgtc ggcaatgtag aacctgaaag tgttgagaaa
     3781 caaatacgtg aaaaatttgc acttaaacca ttcgataaac atcaattcca acattctact
     3841 catcatttac acgatgaaga agttgattat attgttgaat atgatgacgt ggatcaagct
     3901 aaattaaata tgggataccg ttttccaaca caatatggac aaagtggata tgctgccttt
     3961 gttgtattta acatgatgtt tggaggagat ccttcatctg ttttatttaa tgaagtgcga
     4021 gaaaagcaaa gtttagcgta ctctatacat tcacaaattg atggcaaaaa tggctattta
     4081 tttgttttga gtggggtttc aagtgataag tacgaaactg caaaagacac tattataagt
     4141 gaatttgaaa aaataaaagc aggagatttc actgaagaaa aattagagtt agctaaaaaa
     4201 gtaatcattt ctcatcgata tgaatctgaa gatcgtccga aaagtattat agagattatg
     4261 cataaccaaa tattattaga gcaaccacaa agcaaagaaa catttataaa tgatatacag
     4321 aaggtaagtc gcgaagatat tgtttctgtt gctgaaaaag catttttaga tacaatctat
     4381 gtgttgacaa aaggagggga taaataatga aagagcgtta ttatgaatta atagacgaaa
     4441 gagtattcga acaagaatta gaaaatggtt tacgattatt tattatcccc aaaccaggtt
     4501 ttcaaaagac atttgtcact tacactacac aatttggttc attagataat caattcaaac
     4561 cccttggaca agaccaattt gttactgtac ctgatggagt tgctcacttt ttagaacata
     4621 aattatttga aaaagaagaa gaagacttat ttactgcgtt tgctgaagat aacgcacaag
     4681 caaatgcgtt tacaagcttt gatcgtacaa gctacttgtt cagtgcaact gataatattg
     4741 aaaacaacat taaacgttta cttacaatgg ttgaaacgcc ttattttaca aaagaaactg
     4801 ttgataaaga aaaaggtatt attgcagaag aaataaaaat gtatcaagag caacctggat
     4861 ataaattaat gtttgataca ttgcgtgcaa tgtatcaaca acatccaata cgtgttgata
     4921 ttgccggtag t
//
LOCUS       DANRDI010000179         4890 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.180, whole genome shotgun sequence.
ACCESSION   DANRDI010000179 DANRDI010000000
VERSION     DANRDI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.180"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            55..1020
                     /locus_tag="PZ377_002473"
     CDS             55..1020
                     /locus_tag="PZ377_002473"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDM6359329.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            1170..1811
                     /locus_tag="PZ377_002474"
     CDS             1170..1811
                     /locus_tag="PZ377_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDM6359330.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            2122..2694
                     /locus_tag="PZ377_002475"
     CDS             2122..2694
                     /locus_tag="PZ377_002475"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDM6359331.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            2694..>4890
                     /locus_tag="PZ377_002476"
     CDS             2694..>4890
                     /locus_tag="PZ377_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDM6359332.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLL"
BASE COUNT         1774 a          706 c          954 g         1456 t
ORIGIN      
        1 gttacatttt atatataata gtaattgcgt aagtaaataa ttggaggact ataaatgtta
       61 aataatgaat ataagaattc gtcattaaag attttttcat tgaaaggaaa cgaagcatta
      121 gcgcaagaag ttgctgacca agtaggaatt gaactaggta aatgttcagt taaacgtttt
      181 agtgatggag aaattcaaat taatatcgaa gagagtattc gtggttgtga cgtatttatt
      241 attcaaccaa catcatatcc tgtgaatcta catttaatgg aattattaat tatgattgat
      301 gcttgtaaac gtgcttctgc agcaacaatc aatattgtag tgccatatta tggatatgca
      361 agacaagata gaaaagcccg tagccgtgag ccaatcactg ctaaattagt tgcaaactta
      421 atcgaaacag ctggcgcaac tcgtatgatt gcgttagact tacatgcacc acaaattcaa
      481 ggattctttg atattccaat tgaccactta atgggtgtgc caattcttgc taaacatttc
      541 aaagatgatc cgaatattaa cccagaagaa tgtgtcgttg tttcaccaga ccatggcggc
      601 gttacacgtg cacgtaaatt agctgacatt ttaaaaactc caattgcaat tatagataaa
      661 cgtcgtccta gaccaaatgt tgctgaagtg atgaacattg ttggtgagat tgaaggacgt
      721 acggcaatta ttattgacga tattattgat acagcaggta caatcacttt agctgcacaa
      781 gcattaaaag ataaaggtgc taaagaagta tatgcttgtt gtacacaccc tgttttatca
      841 ggaccggcta aagaacgtat cgaaaattct gctataaaag aattaatcgt aacaaactca
      901 attcatttag atgaagatcg caaaccatct aacactaaag aattatctgt tgctggttta
      961 atcgcacaag ctatcattcg tgtatacgaa agagaatcag ttagcgtatt atttgactaa
     1021 tatttaaaag gcgtttgacg aacatattcc aaacgtgtat aatagtttcg ttcgtgatta
     1081 tacgaataaa taaacacttg caagcaacga tgatgttgat gggtaagtga ggtgctcgtt
     1141 ttgagcaaaa atgaaaggtg gaaatgagaa tggcttcatt aaagtcaatc atccgtcaag
     1201 gtaaacaaac acgttcagat cttaaacaat taagaaaatc tggtaaagta ccagcagtag
     1261 tatacggtta cggtactaaa aacgtgtcag ttaaagttga tgaagtagaa ttcatcaaag
     1321 ttatccgtga agtaggtcgt aacggtgtta tcgaattagg cgttggttct aaaactatca
     1381 aagttatggt tgcagactac caattcgatc cacttaaaaa ccaaattact cacattgact
     1441 tcttagcaat caatatgagt gaagaacgta ctgttgaagt accagttcaa ttagttggtg
     1501 aagcagtagg cgctaaagaa ggcggcgtag ttgaacaacc attattcaac ttagaagtaa
     1561 ctgctactcc agacaatatt ccagaagcaa tcgaagtaga cattactgaa ttaaacatta
     1621 acgacagctt aactgttgct gatgttaaag taactggcga cttcaaaatc gaaaacgatt
     1681 cagctgaatc agtagtaaca gtagttgctc caactgaaga agaaatcgaa gctatggaag
     1741 gcgaacaaca aactgaagaa ccagaagttg ttggcgaaag caaagaagac gaagaaaaaa
     1801 ctgaagagta attttaatct gttacattaa agtttttata ctttgtttaa caagcactgt
     1861 gcttatttta atataagcat ggtgcttttt gtgttattat aaagcttaat taaactttat
     1921 tactttgtac taaagtttaa ttaattttag tgagtaaaag acattaaact caacaatgat
     1981 acatcataaa aattttaatg tactcgattt taaaatacat acttactaag ctaaagaata
     2041 atgataattg atggcaatgg cggaaaatgg atgttgtcat tataataata aatgaaacaa
     2101 ttatgttgga ggtaaacacg catgaaatgt attgtaggtc taggtaatat aggtaaacgt
     2161 tttgaactta caagacataa tatcggcttt gaagtcgttg attatatttt agagaaaaat
     2221 aatttttcat tagataaaca aaagtttaaa ggtgcatata caattgaacg aatgaacggc
     2281 gataaagtgt tatttatcga accaatgaca atgatgaatt tgtcaggtga agcagttgca
     2341 ccgattatgg attattacaa tgttaatcca gaagatttaa ttgtcttata tgatgattta
     2401 gatttagaac aaggacaagt tcgcttaaga caaaaaggaa gtgcgggcgg tcacaatggt
     2461 atgaaatcaa ttattaaaat gcttggtaca gaccaattta aacgtattcg tattggtgtg
     2521 ggaagaccaa cgaatggtat gacggtacct gattatgttt tacaacgctt ttcaaatgat
     2581 gaaatggtaa cgatggaaaa agttatcgaa cacgcagcac gcgcaattga aaagtttgtt
     2641 gaaacatcac gatttgacca tgttatgaat gaatttaatg gtgaagtgaa ataatgacaa
     2701 tattgacaac gcttataaaa gaagataatc attttcaaga ccttaatcag gtatttggac
     2761 aagcaaacac actagtaact ggtctttccc cgtcagctaa agtgacgatg attgctgaaa
     2821 aatatgcaca aagtaatcaa cagttattat taattaccaa taatttatac caagcagata
     2881 aattagaaac agatttactt caatttatag atgctgaaga attgtataag tatcctgtgc
     2941 aagatattat gaccgaagag ttttcaacac aaagccctca actgatgagt gaacgtatta
     3001 gaactttaac tgcgttagct caaggtaaga aagggttatt tatcgttcct ttaaatggtt
     3061 tgaaaaagtg gttaactcct gttgaaatgt ggcaaaatca ccaaatgaca ttgcgtgttg
     3121 gtgaggatat cgatgtggac caatttctta acaaattagt taatatgggg tacaaacggg
     3181 aatccgtggt atcgcatatt ggtgaattct cattgcgagg aggtattatc gatatctttc
     3241 cgctaattgg ggaaccaatc agaattgagc tatttgatac cgaaattgat tctattcggg
     3301 attttgatgt tgaaacgcag cgttccaaag ataatgttga agaagtcgat atcacaactg
     3361 caagtgatta tatcattact gaagaagtga tcagccatct taaagaagag ttaaaaactg
     3421 catatgaaaa tacaagaccc aaaatagata aatcagtgcg caatgatttg aaagaaacgt
     3481 atgaaagctt taaattattc gaaagtacat actttgatca tcaaatacta cgtcgcttag
     3541 tagcgtttat gtatgaaaca ccttcgacaa ttattgagta tttccaaaaa gatgcaatca
     3601 ttgcagttga tgaatttaat cgtattaaag aaactgaaga aagtttaaca gtagagtctg
     3661 attcgtttat tagcaatatt attgaaagtg gtaatggatt tataggacaa agttttataa
     3721 aatatgatga ttttgaaaca ttgattgaag gctatcctgt cacttatttt tcattattcg
     3781 ctacaacaat gccgataaaa ctaaatcata ttattaaatt ttcatgtaaa cctgtccaac
     3841 aattttatgg gcaatatgac attatgcgtt ctgaatttca acgatatgtt aatcaaaact
     3901 atcatatcgt ggttttggtc gaaaccgaaa ctaaagttga acgtatgcaa gcgatgttaa
     3961 gtgaaatgca tattccatca ataacaaaat tgcatcgctc aatgtcatcg gggcaagcag
     4021 tgattattga aggcagttta tctgaaggat ttgaactacc tgatatggga ttagttgtca
     4081 ttactgagcg tgagcttttt aaatcaaaac agaaaaagca acgaaaacgt acgaaagcta
     4141 tctcaaatgc tgaaaaaatt aagtcttacc aagatttaaa tgtgggagat tatattgttc
     4201 atgtgcatca tggtgttggt agatatttag gtgttgagac gctcgaagtg gggcaaacgc
     4261 atcgtgatta tattaaattg caatataaag gtacggatca actatttgtt ccagtagatc
     4321 aaatggatca agttcaaaaa tatgtagctt cggaagataa gacgccaaaa ttaaataaac
     4381 tcggtggcag tgaatggaaa aaaacaaaag ctaaagttca acaaagtgtt gaagatattg
     4441 ctgaagagtt gattgattta tataaagaaa gagaaatggc agaaggttat caatatgggg
     4501 aagacacagc tgagcaaaca acatttgaat tagattttcc atatgaactt acgcctgacc
     4561 aagctaaatc tatcgatgaa attaaagatg acatgcaaaa atcgcgtcca atggatcgct
     4621 tgctatgtgg tgatgttggt tatggtaaaa ctgaagttgc agtgagagca gcattcaaag
     4681 ctgtaatgga aggaaagcag gttgcatttt tagttcctac aactatttta gctcagcaac
     4741 attatgagac gttaattgag cgtatgcaag attttcctgt tgaaattcaa ttaatgagtc
     4801 gttttagaac gcctaaagag ataaaacaaa ctaaggaagg acttaaaact ggatttgttg
     4861 acatagttgt tggtacacac aaattactta
//
LOCUS       DANRDI010000180         4654 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.182, whole genome shotgun sequence.
ACCESSION   DANRDI010000180 DANRDI010000000
VERSION     DANRDI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.182"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..368
                     /locus_tag="PZ377_002477"
     CDS             <1..368
                     /locus_tag="PZ377_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
                     /protein_id="HDM6359333.1"
                     /translation="LWNRDVGNIYTGSLYLSLISLLQNHTFQPEEKVCLFSYGSGAVG
                     EIFSGSIVKGYDKALDKEKHLNMLESREQLSVEEYETFFNRFDNQEFDFERELTQDPY
                     SKVYLYSIEDHIRTYKIEK"
     gene            1205..1948
                     /locus_tag="PZ377_002478"
     CDS             1205..1948
                     /locus_tag="PZ377_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDM6359334.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            2045..2473
                     /locus_tag="PZ377_002479"
     CDS             2045..2473
                     /locus_tag="PZ377_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDM6359335.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2519..4528)
                     /gene="mecA"
                     /locus_tag="PZ377_002480"
     CDS             complement(2519..4528)
                     /gene="mecA"
                     /locus_tag="PZ377_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDM6359336.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NDNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
BASE COUNT         1567 a          687 c          762 g         1638 t
ORIGIN      
        1 tattatggaa tagagatgtt ggtaacattt atacaggatc attatactta agtttaattt
       61 cgttattaca gaaccacaca ttccaaccag aagagaaagt atgtctattt agttatggtt
      121 caggagcagt aggagaaatc tttagtggtt caatcgttaa aggatatgac aaagcattag
      181 ataaagagaa acacttaaat atgctagaat ctagagagca attatcagtc gaagaatacg
      241 aaacattctt taacagattt gataatcaag aatttgattt cgaacgtgaa ttgacacaag
      301 atccatattc aaaagtatac ttatacagta tagaagacca tatcagaaca tataagatag
      361 agaaataaac tagtggccga ttgtgcttga tgagcttggg acataaatcc taactcgaaa
      421 taaataagca tatcactaaa ctgatttttt aaagtttaca gtgatatgct tattttttta
      481 tcttacgatt ttgtacgtgc atgcttgcct aggggtatgg ctcgagccat tagtctctcg
      541 cacatactat tccctcaggc gtcagcactt acaaaatcgg ttgtaatttt catttttata
      601 cgcattctta ctgagattat actaataaga ggaatagtaa aagcaattct aagtaaaatt
      661 gcagataaga ggtttgttaa aagcagttct cagtaaaatt acagataaga ggtacgttaa
      721 aagcagttct aagtaaaatt gcagataaga ggtttgttaa aagcagttct aagtaaaatt
      781 gcagataaga ggtacgttaa aagcaattcc atgcaaaatt gctgataagg ggtaagttaa
      841 aagcagttct cagtaaaatt gcagataaga ggtacgttaa aagcagttct aggcaaaatt
      901 gcagataaga ggtgcgttaa aagcagttct cagtaaaatt gctgataagg ggtaagttaa
      961 aagcaatcct aagtaaaatt gcagataagg ggtacagaaa aactagactt gattacaaaa
     1021 tggagcttgg gacataaatg attttttaaa aatgagatga gacgtagatt aactccataa
     1081 tcaatacgaa tctatcgact tctttattta tgatattcat ctctttttaa tggaaataaa
     1141 agtgcgatta atgtgataat acagttacgt taattaaaaa aataaaaatg caaggagagg
     1201 taatatgcta actgtatatg gacatagagg attacctagt aaagctccgg aaaatacaat
     1261 tgcatcattt aaagctgctt cagaagtaga aggtataaac tggttggagt tagatgttgc
     1321 aattacaaaa gatgaacaac tgattatcat tcatgatgat tatttagaac ggactacaaa
     1381 tatgtccggg gaaataactg aattgaatta tgatgaaatt aaagatgctt ctgcaggatc
     1441 ttggtttggt gaaaaattca aagatgaaca tttgccaact ttcgatgatg tagtaaaaat
     1501 agcaaatgaa tataatatga atttaaatgt agaattaaaa ggtattactg gaccgaatgg
     1561 actagcactt tctaaaagta tggttaagca agtggaagaa caattaacaa acttaaatca
     1621 gaatcaagaa gtgctcattt caagctttaa tgttgtgctt gttaaacttg cagaagaaat
     1681 catgccacaa tataacagag cagttatatt ccatacaact tcgtttcgtg aagactggag
     1741 aacactttta gattactgta atgctaaaat agtaaacact gaagatgcca aacttactaa
     1801 agcaaaagta aaaatggtaa aagaagcggg ttatgaattg aacgtatgga ctgtaaacaa
     1861 accagcacgt gcaaaccaac ttgctaattg gggagttgat ggtatcttta cagacaatgc
     1921 agataaaatg gtgcatttgt ctcaatagaa agttagaggt gagtcttacg tttcagtgac
     1981 ggtagactta cctttaacat gttacatact aaaaaattaa tttgaataag aaagagagac
     2041 atatatgaaa tacgatgatt ttatagtagg agaaacattc aaaacaaaaa gccttcatat
     2101 tacagaagaa gaaattatcc aatttgcaac aacttttgat cctcaatata tgcatataga
     2161 taaagaaaaa gcagaacaaa gtagatttaa aggtatcatt gcatctggca tgcatacact
     2221 ttcaatatca tttaaattat gggtagaaga aggtaaatac ggagaagaag ttgtagcagg
     2281 aacacaaatg aataacgtta aatttattaa acctgtatac ccaggtaata cattgtacgt
     2341 tatcgctgaa attacaaata agaaatccat aaaaaaagaa aatggactcg ttacagtgtc
     2401 actttcaaca tacaatgaaa atgaagaaat tgtatttaag ggagaagtaa cagcacttat
     2461 taataattca taataaaaca gtgaagcaac catcgttacg gattgcttca ctgttttgtt
     2521 attcatctat atcgtatttt ttattaccgt tctcatatag ctcatcatac actttacctg
     2581 agattttggc attgtagcta gccattcctt tatcttgtac atctttaaca ttaatagcca
     2641 tcatcatgtt tggattatct ttatcatatg atataaacca cccaatttgt ctgccagttt
     2701 ctccttgttt cattttgagt tctgcagtac cggatttgcc aattaagttt gcataagatc
     2761 tataaatatc ttctttatgt gttttattta cgacttgttg cataccatca gttaatagat
     2821 tgatattttc tttggaaata atatttttct tccaaacttt gtttttcgtg tcttttaata
     2881 agtgaggtgc gttaatattg ccattatttt ctaatgcgct atagattgaa aggatctgta
     2941 ctgggttaat cagtatttca ccttgtccgt aacctgaatc agctaataat atttcattat
     3001 ctaaattttt gtttgaaatt tgagcattat aaaatggata atcacttggt atatcttcac
     3061 caacacctag ttttttcatg cctttttcaa atttcttact gcctaattcg agtgctactc
     3121 tagcaaagaa aatgttatct gatgattcta ttgcttgttt taagtcgata ttatcattta
     3181 ccacttcata tcttgtaacg ttgtaaccac cccaagattt atctttttgc caacctttac
     3241 catcgatttt ataacttgtt ttatcgtcta atgttttgtt atttaaccca atcattgctg
     3301 ttaatatttt ttgagttgaa cctggtgaag ttgtaatctg gaacttgttg agcagaggtt
     3361 cttttttatc ttcggttaat ttattatatt cttcgttact catgccatac ataaatggat
     3421 agacgtcata tgaaggtgtg cttacaagtg ctaataattc acctgtttga gggtggatag
     3481 cagtacctga gccataatca tttttcatgt tgttataaat actcttttga actttagcat
     3541 caatagttag ttgaatatct ttgccatctt ttttcttttt ctctattaat gtatgtgcga
     3601 ttgtattgct attatcgtca acgattgtga cacgatagcc atcttcatgt tggagctttt
     3661 tatcgtaaag tttttcgagt ccctttttac caataactgc atcatcttta tagcctttat
     3721 attctttttg ttttaattct tcagagttaa tgggaccaac ataacctaat agatgtgaag
     3781 tcgcttttcc tagaggatag ttacgacttt ctgtttcatt agttgtaaga tgaaattttt
     3841 ttgcgaaatc acttaaatat tcatccattt ttttaacggt tttaagtgga acgaaggtat
     3901 catcttgtac ccaattttga tccatttgtt gtttgatata gtcttcagaa atacttagtt
     3961 ctttagcgat tgctttataa tcttttttag atacattctt tggaacgatg cctatctcat
     4021 atgctgttcc tgtattggcc aattccacat tgtttcggtc taaaatttta ccacgttctg
     4081 attttaaatt ttcaatatgt atgctttggt ctttctgcat tcctggaata atgacgctat
     4141 gatcccaatc taacttccac ataccatctt ctttaacaaa attaaattga acgttgcgat
     4201 caatgttacc gtagtttgtt ttaattttat attgagcatc tactcgtttt ttatttttag
     4261 atactttttt tattttacga tcctgaatgt ttatatcttt aacgcctaaa ctattatata
     4321 tttttatcgg acgttcagtc atttctactt caccattatc gcttttagaa atataactgc
     4381 tatctttata aacttgtttg aaatttttat cttcaattgc atcaatagta ttattaattt
     4441 ctttatcttt tgaagcataa aaatatatac caaacccgac aactacaact attaaaataa
     4501 gtggaacaat ttttatcttt ttcatcaata tactccttat ataagactac atttgtagta
     4561 tattacaaat gtagtattta tgtcaaaata atgttataat ttttgtgata tggaggtgta
     4621 gaaggtgtta tcatcttttt taatgttaag tata
//
LOCUS       DANRDI010000181         4621 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.183, whole genome shotgun sequence.
ACCESSION   DANRDI010000181 DANRDI010000000
VERSION     DANRDI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.183"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1254)
                     /locus_tag="PZ377_002481"
     CDS             complement(<1..1254)
                     /locus_tag="PZ377_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDM6359337.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGH"
     gene            1402..1587
                     /locus_tag="PZ377_002482"
     CDS             1402..1587
                     /locus_tag="PZ377_002482"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDM6359338.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(1675..2658)
                     /locus_tag="PZ377_002483"
     CDS             complement(1675..2658)
                     /locus_tag="PZ377_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDM6359339.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            2795..3304
                     /gene="msrA"
                     /locus_tag="PZ377_002484"
     CDS             2795..3304
                     /gene="msrA"
                     /locus_tag="PZ377_002484"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDM6359340.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(3507..>4621)
                     /locus_tag="PZ377_002485"
     CDS             complement(3507..>4621)
                     /locus_tag="PZ377_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDM6359341.1"
                     /translation="GTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAWLFDYQF
                     SKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKASSLVVLG
                     DPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGEEAIIDL
                     TQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDNRQEMHF
                     SVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELDLPLMDG
                     LYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYRFQGLRR
                     YKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
BASE COUNT         1534 a          857 c          655 g         1575 t
ORIGIN      
        1 atgtccagcc attaaaccta atctgccgtg ttttgtacct gcttctgtaa acgatactgc
       61 tttggacaca atacctttgc cgtatttcaa atgtaattgg tctatcgttt tagctagaca
      121 ttcgtcacgt ttgcgttggt attcatcttc aaacagactt aactgtcgct catcctcatt
      181 aataaattga ctcaaagata tacttagcgt acgatatagt gcttgtttat tacataattt
      241 atctgcgaaa tgcattacta ctttataaat atctttttct aagtttgttg gatctttcaa
      301 agtatattgc ttatgtacac cgccttcatc actatagcca aaggcaaaat gtatcgttct
      361 tgccactttt tttcttgctc gaactctgct agcaacatct tcaattaact cttgcattac
      421 tacttttgct tcatcaaaat gataatctct cattaatatt tgacttttgc atatcgatgg
      481 attgctgatc ttgtgctttt cacgtacttt actctgatct atcccattcg catgtagatg
      541 catatcaaca cctaaaatac cgaactcttt ttttaaaaat ttatatggat atttcgctaa
      601 atctcctata gtaaaaattc ctcttttatt caatttggct tctgttcgac gattaatacc
      661 ccaaaaatct cgcaagggct gaattggcca taatttcgtt ggtacatctt gatatcgcca
      721 ttcagctata ccattttgac tatgcttcgc ttcaacatcc atagcaattt tacttaataa
      781 catattagaa ccaatgccca cagtacaata aatgcctgtt tcttcataaa tttcacgttt
      841 aagtctttcg caaaatgcat gtactgtaga actaaatcta tgatagctat cagtaacatc
      901 cataaaaaat tcgtcaatac tatattggtg taaatcttca ggtggaatat aacgcaatgc
      961 aatcttagaa atagcaactg aaacattaag atatttacgc atacttggat tgataatgta
     1021 aatatcattt ctatgtggta tttcaaacaa tcgcgaccct gtcttgatgc ctaattcttt
     1081 taatttaggt gtcgcagcca atattacaga accctgacgc ttagtatctg caacaacagc
     1141 tagctttgtt tctaatggat ctagcccctt ttcaatacaa gaaacactcg caaaaaaact
     1201 tttttggtca atacatagaa catccctatc ttctaataaa tgataattat acactgttat
     1261 tcctccctta cttgaatctt attatatacg aacacgtgtt ctatttaaac tattttagaa
     1321 caaatgttct attattttgc aaatgaattt ttaagtatta catgatatac tcaaattaat
     1381 aatgattagg aggtttggat gatgccaatc gtcaatgtaa aattattaga aggtcgttcg
     1441 gatgaacaat taaaaaattt agttagcgaa gtaactgacg ccgtagaaaa aacaacgggg
     1501 gcaaatagac aagcaattca cgttgttata gaagaaatga aaccaaacca ttatggtgtg
     1561 gctggcgtaa gaaagtcaga tcaataattc ttcataagat gcatgccaat taattctttg
     1621 aaaacgaaca aggcgacttc tatctgagta tgatagaaat cgccttgttt atttttaatc
     1681 ttcatctaaa aagtctttaa tagcttgttt atttgtgttt ttattaatct gtaatgcact
     1741 accatcagta tttgtattga catcttcgta tgagttcttg attggcacag tcaatgactt
     1801 aacatctttt tcacctcgga taccaaaact caaacctgtt tggaaaatcc ctgaatcagg
     1861 aatgtttgta ttcacatagc ctcttaaaat acctgcaact tttggtaatt taacaactgt
     1921 tctaaaatta accatttctt ttttcaatgt ttgcatcact tgttgctgac gtcgcacgcg
     1981 tccgaagtca ccttcagggt cgtgacggaa tcttgcataa ccaagtaatt ctttaccatt
     2041 caacctatgg ttaccctttt tcaaagatac accaatattt ttcgacatat ctttttcgac
     2101 attaattggt acaccttcag gcattaattc atcaatcatt ttctcaaatc cagtaaaatc
     2161 aactactgca taatattcag gattaattcc taaattttta tcaagtgttt ttctaagtag
     2221 ctctggacca cctaaagcgt atgctgaatt aattttgtgt tttccatatc ctggaatatc
     2281 tgcataaata tcacgcatga cagacatcat tttcatcttt ttattgataa agtcatattg
     2341 aacaaccatg atagaatctg ttcttgattg tccaccttgt gctttatctg caccgagtac
     2401 aagaatagaa attttaccat cattttttac tggtccatta aattgatgta ctttaacatc
     2461 tttcgcatgt ttcttggcat attctacacc gctattgtaa ctatgtacaa tatatacaac
     2521 taatgcgata agtaaaatta caacaatcag aagaatgata ggtaatttcc taattttctt
     2581 cttctttttt cgcttaggcg ccgaagtgcg atgttcactt tgacgtctat attcgttgtc
     2641 attagtttct ttatccatat ttacctacct tatatcttca aaaatagatt tttgtgtact
     2701 ataataatca catattaacg ttatataaaa acaattcaaa aagcaagtga tttcttaata
     2761 aatcgtacta aggtctaatg aaaggatgtc aaaaatgaat attaatacag cttattttgc
     2821 cggaggttgc ttttggtgta tgacgaaacc atttgacacc tttgacggca tagaaaaagt
     2881 aacttctgga tatatgggcg gacatattga aaatcctact tacgaacaag taaaatcagg
     2941 tacgagtggt catttagaaa ctgttgaaat tcaatatgat gttgcattat tctcatacaa
     3001 taagttatta gaaatatttt tctcagtcat tgacccatta gatacaggtg gtcaatatca
     3061 agaccgtggt cctcaatatc aaacagctat tttctacact aatgatcatc aaaaagaact
     3121 cgctgagact tatatcgagc agcttaaaaa tacgattaat gctgataagg caattgcaac
     3181 aaaaatatta ccagcgtcac aattttacaa agccgaagac tatcaccaag atttttataa
     3241 gaaaaatcca gagcgctatg cagaagaaca aaaaatacgc caagaataca aaaataagca
     3301 ataacattta tacttgatga aactgattgg actgataata aaagttaggc gacttaactt
     3361 tagctcattt caatattaaa atacggtaca gataggaaac ttaaatattc ttatctgtac
     3421 cgttttttat aaatcattct ttatagaaac caaaaaatcc aagcgcatca ggcataactg
     3481 tatacctctt agcacttgga ttttaattat ttgtgacgta ttacacgcat tactttagaa
     3541 agtgattccc aaagcgaatt atctttacga taaactaaaa agcgtggttc ccaattcgga
     3601 ttatatttag atttataacg acgtaatcct tggaaacgat atagaccgtt gaaatgttca
     3661 aagacacggc ctgcaagtcg ttctcttaaa tatgaataat gcaattgacc aacgttcgat
     3721 aacgttgcca tacccatatt aaattttgta taaccttgtt ctttactcca aagtaacata
     3781 tgcaagtata gaccatccat taatggtaaa tctaactctg gcaaccatct aattaaatcg
     3841 actgaaatgg catcattaaa gtatgttggc attaaactac aaaatgcaat tacttcattt
     3901 tcttcatttc gcattacacc aattggcgct ttagataagt attcttcatt aaattcacca
     3961 acagagaaat gcatttcctg acgattatct agccataaat cacttacatg ttgaagttca
     4021 tttataaatt cagttgaaaa cggtggttca ataatttcga acgaaatatt aagttcatcg
     4081 aatttattta aagtcgctct aaatccacgg cgttttttac ctgaagttga aaattgcgtt
     4141 aaatcaataa ttgcttcttc acctaatttg aaaaattggt taccgaaatt atgatataaa
     4201 ggcatgtgtt gatctgtaac ttgatagaat ataacatcat agcctaaata ctcagcgtaa
     4261 ttatagaatg cttctaacaa ttcatcaaag gcattttcat cacctaacgg atctccaaga
     4321 accactaatg aacttgcttt ataacgatac attaaaaatg ctgttttatt ttcattagtg
     4381 aaaaactgct tgtcaccact atatatcaag tgactcaaat aattaccgcc gtactgatta
     4441 ataatctcct cgcaatcttc aattttagaa gaaatacgta ctttgctaaa ttgataatca
     4501 aacaaccatg caatcatacc tatgatgata gcgatgatta aaatcgtaag ccagaagtaa
     4561 tagcgcaata cagatgtatg catttcaatc gtataaatat ctaatgcata taacgttcca
     4621 g
//
LOCUS       DANRDI010000182         4540 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.184, whole genome shotgun sequence.
ACCESSION   DANRDI010000182 DANRDI010000000
VERSION     DANRDI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.184"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            386..2449
                     /locus_tag="PZ377_002486"
     CDS             386..2449
                     /locus_tag="PZ377_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDM6359342.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            2467..3012
                     /locus_tag="PZ377_002487"
     CDS             2467..3012
                     /locus_tag="PZ377_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HDM6359343.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            complement(3440..3574)
                     /locus_tag="PZ377_002488"
     CDS             complement(3440..3574)
                     /locus_tag="PZ377_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359344.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            3538..3630
                     /locus_tag="PZ377_002489"
     CDS             3538..3630
                     /locus_tag="PZ377_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359345.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
     gene            3777..>4540
                     /locus_tag="PZ377_002490"
     CDS             3777..>4540
                     /locus_tag="PZ377_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HDM6359346.1"
                     /translation="MKINIYKSIYNFQETNTNFLENLESLNDDNYELLNDKELVSDSN
                     ELKLISKVYIRKKDKKLLDWQLLIKNVYLDTEEDDNLFSESGHHFDAILFLKEDTTLQ
                     NNVYIIPFGQAYHDINNLIDYDFGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNYRR
                     NSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKEIN
                     AIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKLS"
BASE COUNT         1721 a          578 c          788 g         1453 t
ORIGIN      
        1 ctgagcataa atactagaaa aatggccagt aaatgagttt actataaact catttactgg
       61 cttctctaat aattatcaag acaatttgcg tttctaggca tactttgaaa tgcgctattt
      121 tctttaagaa tattaatata tgacttgtgt tggtaatgat aaccttggtt agagtgtatt
      181 gtcatgtgat agttaagatt agatctttta attaaaacat tgttttaaat atcgatgaca
      241 aggtctaatg taggacgtgt agatataagg ttcggaatta tataaatcca taaaaggaga
      301 taaatcgaaa acctttatac cctgggtggg tatatgttat agtataagta gctttactat
      361 aacattttca ttaggagggg ttaatttgaa taataatggt gaagagcata atcatcaaaa
      421 tcacatgaat cattccaatc aaatgcatca tgataaccat gcctcacatg atcatcatag
      481 tggccatgca catcatcatg gaaattttaa agttaagttt tttgtttcat taatttttgc
      541 aatacctatc attcttttat cgccactgat gggtgttaac ttaccttttc aattcacatt
      601 tccaggttct gaatgggtag tgttaatatt aagtacaatt ttattctttt atggtggtaa
      661 accgttcttg tctggtggta aagatgaaat tgctacaaaa aaaccaggca tgatgacctt
      721 agttgcccta ggtatttcag tagcttatat ttatagcttg tatgcttttt atatgaataa
      781 ctttagtagt gcaactggtc atacaatgga ctttttttgg gaattagcaa ccttaatttt
      841 aattatgcta ttaggacatt ggatagaaat gaatgctgtc ggaaatgctg gagatgcttt
      901 aaagaaaatg gcagaactgt tacctaatag tgctattaaa gttatggata atggccaacg
      961 cgaagaagtt aaaatatcag acatcatgac tgatgatatc gtcgaagtaa aagccggaga
     1021 aagcattcca acagatggta ttatcgttca aggacaaaca tctatagatg aatccctagt
     1081 cactggagaa tctaaaaaag tacaaaaaaa tcaaaatgac aacgtcatcg ggggttctat
     1141 taatgggtct ggaacaatac aagtcaaggt tacagctgtg ggagaagatg gatatctttc
     1201 tcaagttatg ggacttgtta atcaagcaca aaatgataaa tctagtgctg aattgttatc
     1261 tgataaagta gcgggttatt tattctactt tgctgtaagt gttggcgtga tttcttttat
     1321 tgtctggatg ctcattcaaa atgatgttga ttttgcatta gaacgtcttg taactgtgtt
     1381 agtcattgct tgtccacatg ctttaggctt ggcaatacct ttagtcactg cacgttctac
     1441 ttcaattggt gcacataatg gtttaattat taaaaataga gagtctgtag aaatagctca
     1501 acatatcgat tatgtaatga tggacaaaac tggtacttta actgagggta acttttctgt
     1561 gaatcattat gagagcttta aaaatgattt gagtaatgat acaatattaa gccttttcgc
     1621 ctcattagaa agtcaatcta atcacccatt agctataagt attgttgatt ttgcgaaaag
     1681 taaaaatgtt tcatttacta acccacaaga cgttaataat attccaggtg tcggattaga
     1741 aggtctaatt gataataaaa catataaaat aacaaatgtc tcttatcttg ataaacataa
     1801 acttaattat gacgatgact tgtttactaa attagctcaa caaggtaatt caatcagtta
     1861 tttaattgag gatcaacaag tcattggcat gattgctcaa ggagatcaaa ttaaagaaag
     1921 ctcaaaacaa atggtagctg atttactatc aagaaatatt acaccagtca tgcttacagg
     1981 tgacaataat gaagtggcac acgctgtcgc aaaagaatta ggtattagtg atgtccacgc
     2041 acaactcatg ccagaagata aggaaagcat tataaaagat tatcaaagtg acggtaataa
     2101 agtcatgatg gtcggagacg gtatcaacga tgcgccgagt cttataagag cggatattgg
     2161 tatagcaatt ggtgcaggta cagatgttgc agtggattca ggtgatatca tacttgttaa
     2221 aagtaatcca tcagatatca ttcatttctt gaccctttca aataatacta tgagaaaaat
     2281 ggtgcaaaac ttatggtggg gtgcaggtta taatattgtt gctgtacctt tagcagctgg
     2341 tattttagca tttattggct tgattttatc acctgcaata ggtgctattt taatgtcttt
     2401 aagtacaatt atcgttgcaa ttaatgcctt tacattaaaa ttaaaataaa agataggagt
     2461 tttattatga ttaaaaaatt attttttatg atattaggat cattactaat attatcagct
     2521 tgctccaata atgatgaaaa agataaagac actaatgacc aaaaaagtga gagccatatg
     2581 aagcataatg atgaaagtaa agttccagaa gatatgaaat cgactaatga gggtgaattt
     2641 aaagtgggag ataaagtaac gattacagca gggcatatgc caggtatgaa aggtgcagaa
     2701 gctactgtaa aaggtgcgta taaaacatat gcctatgttg taagttataa acccacaaat
     2761 ggaaatgaaa aagtaaacaa tcataaatgg gtcgtaaacg aagagattaa agatgcacct
     2821 aaagatggat ttagtaaggg cgatactgtt aaattagaag caagtcatat gtctggtatg
     2881 aaaggtgcta cagccaatat agataacgtg aaaaagacta ctgtttacgt agttgattac
     2941 aaatccaaag ataatggtaa aatcattaaa aatcataaat ggatgacagg aaatgagctg
     3001 aaagcacgat aaaaatctag ttctaaattg agaaataaat agatataaaa atatcctcct
     3061 taatcaataa tttaaataac ttattattgt taaggaggat attttttagt gtgtaaatta
     3121 aaaagaattt tagaagaata acatttatca aaaaactgtt cattacctta ttaattgaaa
     3181 ttatataatt aaaaaccgca tcattaaccg atacgcagag gcgtatcata agtaaaacta
     3241 aaaaattctg tatgaggaga taataatttg gagggtgtta aatggtggac attaaatcca
     3301 cgttcattca atatataaga tatatcacga taattgcgca tataacttaa gtagtagcta
     3361 acagttgaaa ttaggcccta tcaaattggt ttatatctaa aatgattaat atagaatgct
     3421 tctttttgtc cttattaaat tataaaagta actttgcaat agaaacagtt atttcataat
     3481 caacagtcat tgacgtagct aagtaatgat aaataatcat aaataaaatt acagatattg
     3541 acaaaaaata gtaaatatac caatgaagtt tcaaaagaac aattccaaga aattgagaat
     3601 gtaaataata aggtcaaaga attttattaa gatttgaaag agtatcaatc aagaaagatg
     3661 tagtttttta ataaactatt tggaaaataa ttatcataat ttaaaaactg acaatttgcg
     3721 agactcataa aatgtaataa tggaaataga tgtaaaatat aattaagggg tgtaatatga
     3781 agattaatat ttataaatct atttataatt ttcaggaaac aaatacaaat tttttagaga
     3841 atctagaatc tttaaatgat gacaattatg aactgcttaa tgataaagaa cttgttagtg
     3901 attcaaatga attaaaatta attagtaaag tttatatacg taaaaaagac aaaaaactat
     3961 tagattggca attattaata aagaatgtat acctagatac tgaagaagat gacaatttat
     4021 tttcagaatc cggtcatcat tttgatgcaa tattatttct caaagaagat acaacattac
     4081 aaaataatgt atatattata ccttttggac aagcatatca tgatataaat aatttgattg
     4141 attatgactt cggaattgat tttgcagaaa gagcaatcaa aaatgaagac atagttaata
     4201 aaaatgttaa tttttttcaa caaaacaggc ttaaagagat tgttaattat agaaggaata
     4261 gtgtagatta cgttagacct tcagaatctt atatatcagt ccaaggacat ccacagaatc
     4321 ctcaaatttt tggaaaaaca atgacttgtg gtacaagtat ttcattgcgt gtaccgaata
     4381 gaaagcagca attcatagat aaaattagtg tgataatcaa agaaataaac gctattatta
     4441 atcttcctca aaaaattagt gaatttccta gaatagtaac tttaaaagac ttgaataaaa
     4501 tagaagtatt agatacttta ttgctaaaaa aactatcgaa
//
LOCUS       DANRDI010000183         4521 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.185, whole genome shotgun sequence.
ACCESSION   DANRDI010000183 DANRDI010000000
VERSION     DANRDI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.185"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            227..1138
                     /gene="rapZ"
                     /locus_tag="PZ377_002491"
     CDS             227..1138
                     /gene="rapZ"
                     /locus_tag="PZ377_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDM6359347.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1135..2130
                     /locus_tag="PZ377_002492"
     CDS             1135..2130
                     /locus_tag="PZ377_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDM6359348.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2240..3184
                     /gene="whiA"
                     /locus_tag="PZ377_002493"
     CDS             2240..3184
                     /gene="whiA"
                     /locus_tag="PZ377_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDM6359349.1"
                     /translation="MSFASEMKNELTRIDVEEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3758..4345
                     /gene="clpP"
                     /locus_tag="PZ377_002494"
     CDS             3758..4345
                     /gene="clpP"
                     /locus_tag="PZ377_002494"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDM6359350.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
BASE COUNT         1695 a          591 c          867 g         1368 t
ORIGIN      
        1 ttgaaaaaag cttgttgcaa gcgcattttc attcagtcaa ctactagcaa tataatatta
       61 tagaccctag gacattgatt tatgtcccaa gctcctttta aatgatgtat atttttagaa
      121 atttaatcta gacatagttg gaaataaata taaaacatcg ttgcttaatt ttgtcataga
      181 acatttaaat taacatcatg aaattcgttt tggcggtgaa aaaataatgg ataataatga
      241 aaaagaaaaa agtaaaagtg aactattagt tgtaacaggt ttatctggcg caggtaaatc
      301 tttggttatt caatgtttag aagacatggg atatttttgt gtagataatc taccaccagt
      361 gttattgcct aaatttgtag agttgatgga acaaggaaat ccatccttaa gaaaagtggc
      421 aattgcaatt gatttaagag gtaaggaact atttaattca ttagttgcag tagtggataa
      481 agtcaaaagt gaaagtgacg tcatcattga tgttatgttt ttagaagcaa gtactgaaaa
      541 attaatttca agatataagg aaacgcgtcg tgcacatcct ttgatggaac aaggtaaaag
      601 atcgttaatc aatgcaatta atgatgagcg agagcatttg tctcaaatta gaagtatagc
      661 taattttgtt atagatacta caaagttatc acctaaagaa ttaaaagaac gcattcgtcg
      721 atactatgaa gatgaagagt ttgaaacttt tacaattaat gtcacaagtt tcggttttaa
      781 acatgggatt cagatggatg cagatttagt atttgatgta cgatttttac caaatccata
      841 ttatgtagta gatttaagac ctttaacagg attagataaa gacgtttata attatgttat
      901 gaaatggaaa gagacggaga ttttctttga aaaattaact gatttgttag attttatgat
      961 acccgggtat aaaaaagaag ggaaatctca attagtaatt gccatcggtt gtacgggtgg
     1021 acaacatcga tctgtagcat tagcagaacg actaggtaat tatctaaatg aagtatttga
     1081 atataatgtt tatgtgcatc atagggacgc acatattgaa agtggcgaga aaaaatgaga
     1141 caaataaaag ttgtacttat cggtggtggc actggcttat cagttatggc taggggatta
     1201 agagaattcc caattgatat tacggcgatt gtaacagttg ctgataatgg tgggagtaca
     1261 gggaaaatca gagatgaaat ggatatacca gcaccaggag acatcagaaa tgtgattgca
     1321 gctttaagtg attctgagtc agttttaagc caactttttc agtatcgctt tgaagaaaat
     1381 caaattagcg gtcactcatt aggtaattta ttaatcgcag gtatgactaa tattacgaat
     1441 gatttcggac atgccattaa agcattaagt aaaattttaa atattaaagg tagagtcatt
     1501 ccatctacaa atacaagtgt gcaattaaat gctgttatgg aagatggaga aattgttttt
     1561 ggagaaacaa atattcctaa aaaacataaa aaaattgatc gtgtgttttt agaacctaac
     1621 gatgtgcaac caatggaaga agcaatcgat gctttaaggg aagcagattt aatcgttctt
     1681 ggaccagggt cattatatac gagcgttatt tctaacttat gtgtgaatgg tatttcagat
     1741 gcgttaattc attctgatgc gcctaagcta tatgtttcta atgtgatgac gcaacctggg
     1801 gaaacagatg gttatagcgt gaaagatcat atcgatgcga ttcatagaca agctggacaa
     1861 ccgtttattg attatgtcat ttgtagtaca caaactttca atgctcaagt tttgaaaaaa
     1921 tatgaagaaa aacattctaa accagttgaa gttaataagg ctgaacttga aaaagaaagc
     1981 ataaatgtaa aaacatcttc aaatttagtt gaaatttctg aaaatcattt agtaagacat
     2041 aatactaaag tgttatcgac aatgatttat gacatagctt tagaattaat tagtactatt
     2101 cctttcgtac caagtgataa acgtaaataa tatagaacgt aatcatatta tgatatgata
     2161 atagagctgt gaaaaaaatg aaaatagaca gtggttctaa ggtgaatcat gttttaaata
     2221 agaaaggaat gactgtacga tgagctttgc atcagaaatg aaaaatgaat taactagaat
     2281 agacgtcgag gaaatgaatg caaaagcaga gctcagtgca ctgattcgaa tgaatggtgc
     2341 acttagtctt tcaaatcaac aatttgttat aaatgttcaa acggaaaatg caacaacggc
     2401 aagacgtatt tattcgttga ttaaacgtgt ctttaatgtg gaagttgaaa tattagtccg
     2461 taaaaaaatg aaacttaaaa aaaataatat ttatatttgt cgtacaaaga tgaaagcgaa
     2521 agaaattctt gatgaattag gaattttaaa agacggcatt tttacgcatg aaattgatca
     2581 ttcaatgatt caagatgacg aaatgagacg cagttacttg agaggagctt ttctggcagg
     2641 tggctcagtg aataaccctg aaacatcttc gtaccatttg gaaatttttt ctcaaaatga
     2701 gagtcatgca gaaggcttaa cgaaactaat gaatagttat gagttgaatg ccaaacattt
     2761 agagcgaaaa aaaggaagta ttacgtattt aaaagaagcg gaaaagattt cggattttct
     2821 tagtttgata ggtggctatc aagcgttatt aaaatttgaa gacgtacgta ttgtaagaga
     2881 tatgcgtaat tctgttaacc gactcgttaa ttgtgaaacg gccaatctaa ataaaacagt
     2941 tagtgctgcg atgaaacaag ttgagagcat taaattgatt gataaagaaa ttggtattga
     3001 aaatttacca gacaggttga gagagattgc tagaattcga gtagaacatc aagaaatttc
     3061 gttgaaagag cttggagaaa tggtatcaac tggtccaatt tcaaaatcag gtgtaaatca
     3121 ccgattaaga aaactgaatg atttagccga taagattaga aatggtgaac aaatagaatt
     3181 ataagtaaga aggtgttttt ggtagtgaat tatcaaaaac acttttttta tgattaaaaa
     3241 gtgtttaatg aataatagtc gactaactat taaattgagc tggaaagttt aatgaaggaa
     3301 attcaataac caagtgatgc ctaattttct cattaaatag catcatatgt atcaatttgg
     3361 tcataagaaa atatgaatgt gataattaat tgagtccctg aaagtccctg atcatagaca
     3421 aaaacaaaat cagcataaat aagaatcccg acattgcggg attctctgta ttgaaaagta
     3481 ttgtatttta ttaaaacagc ctccttgaag ggaattgaac ccctatctta agaaccggaa
     3541 tcttacgtgt tatccattac accacaagga gcaaatatgt aatcatatct ctgtactatg
     3601 agaagtaaaa taagtacaat ctaaaataga tattatcagt ttacaaagga aagagaaaag
     3661 cgtcaaacaa tgtaactatt taaagtcaaa gtgtttgacc aaatttgact taatatgtaa
     3721 aataatgagt aacagttatt acaaggagga aatatagatg aatttaattc ctacagttat
     3781 tgaaacaaca aaccgcggtg aacgtgcata tgatatatac tcacgtttat taaaagaccg
     3841 tattattatg ttaggttcac aaattgatga caacgtagca aattcaatcg tatcacagtt
     3901 attattctta caagcgcaag actcagagaa agatatttat ttatacatta attcaccagg
     3961 tggaagtgta acagctggtt ttgcgattta tgatacaatt caacacatta aacctgatgt
     4021 tcaaacaatt tgtatcggta tggctgcatc aatgggatca ttcttattag cagctggtgc
     4081 aaaaggtaaa cgtttcgcgt taccaaatgc agaagtaatg attcaccaac cattaggtgg
     4141 tgctcaagga caagcaactg aaatcgaaat tgctgcaaat cacattttaa aaacacgtga
     4201 aaaattaaac cgcattttat cagagcgtac tggtcaaagt attgaaaaaa tacaaaaaga
     4261 cacagatcgt gataacttct taactgcaga agaagctaaa gaatatggct taattgatga
     4321 agtgatggta cctgaaacaa aataattcaa agtaaagagt agactaagct gtctgctctt
     4381 tttgtatgag taaaccaagg tgtcaataat ttgtttacta tactttgagc ggaaatatga
     4441 ttgaatgaag ctagttgaac cgtaactata tgaaatgttc ccttcaaagt agacattgaa
     4501 aggaacattt caatcctttg t
//
LOCUS       DANRDI010000184         4467 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.186, whole genome shotgun sequence.
ACCESSION   DANRDI010000184 DANRDI010000000
VERSION     DANRDI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.186"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            149..325
                     /locus_tag="PZ377_002495"
     CDS             149..325
                     /locus_tag="PZ377_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDM6359351.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            340..942
                     /locus_tag="PZ377_002496"
     CDS             340..942
                     /locus_tag="PZ377_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDM6359352.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            1175..1320
                     /locus_tag="PZ377_002497"
                     /pseudo
     CDS             1175..1320
                     /locus_tag="PZ377_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            1859..1960
                     /locus_tag="PZ377_002498"
     CDS             1859..1960
                     /locus_tag="PZ377_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359353.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            1970..2242
                     /locus_tag="PZ377_002499"
     CDS             1970..2242
                     /locus_tag="PZ377_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDM6359354.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            2286..4250
                     /locus_tag="PZ377_002500"
     CDS             2286..4250
                     /locus_tag="PZ377_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDM6359355.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKEN
                     KLSIQFINKNDCRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIIL
                     FDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVIFIGILTNVILHSHYIMML
                     FATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            4253..>4467
                     /locus_tag="PZ377_002501"
     CDS             4253..>4467
                     /locus_tag="PZ377_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDM6359356.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNG"
BASE COUNT         1629 a          548 c          699 g         1591 t
ORIGIN      
        1 actttgctca aaatgccaat tatatactca caaatcattc taaatatcgg cttaaagtta
       61 cattttatta tttttgattg ttaatagttt atttgttaag tgaattttag tacaatataa
      121 tttagttatg atttaaggag gacttaaaat gactgttgca gaagtgggta acattgttga
      181 gtttatggat ggattaagag gtcgtgttga aaaaatcaac gataactctg ttattgttga
      241 cttaacaatt atggaaaatt ttaatgacct tgatttaccg gaaaaaactg ttatcaatca
      301 taaacgatat aagattgttg aataagaagg taagttataa tgaataaaat ctcgaaggct
      361 ttaacttggt ttattataag tttcattata tttcatctca tattatttat tatgtggggc
      421 gaacaccaag aatactggta tttatataca ggtataatgc taattgctgg tatcagttat
      481 gtattttatc aaagagatat tgaatctaag cggttgctta catcaattgg tgttggtatt
      541 attacggcaa ttattttaat tatgcttcaa cttttattct cacttataac ttctaattta
      601 agttatagtt cattaattaa agaattagca agaacaggtg tcaattggaa gtggcaaatg
      661 ttagtaactt tactttttgt cattccatgt cacgagttat atatgagaac tgttttacaa
      721 aaagaattaa cacatttttc aataccgaaa tgggtagcta tattaataac tgcaatttgt
      781 tctagttcat tatttattta tttagataat tggtggatag taacatttat atttgttgca
      841 caagtgatat tatcattaag ttatgagtat actcgacgta tagcaacaac atccgttgca
      901 caaattgtag ctattatatt gttattaatt tttaatgctt aaaaaagctt ttcttagacg
      961 tatgtgttct aagaaaagct tttttgctta agaatagcaa tacgaagatt cgaggcatgc
     1021 ttcattatag tggtaatatt ttttaacagc aagcggttat tattgagatt aggttttagt
     1081 cagtttttat tactaaatat tttataaaaa tggtataata tttttgagca agttgaattg
     1141 atggtggcta tctgagtaaa ggggggtggt gcctatggca ttacttaaca cttttgaaag
     1201 gaaaagccta ttgtgatatc tattgcaaac gcattacatt taatgttaag tttcggtatg
     1261 tttatcgtca ctttcattgg tatagtagta gcaataataa atttaagcaa taaaaaataa
     1321 ccatcatttc aaaactttga catagaatga tggttaaaga aaatctcatt tagtcaccgt
     1381 ctttttaacg ggctcattag gcgacatgtt cctgcatgtc gtctttttac atttttatag
     1441 taacatatct tttatttaat tgtctatttt tattagatgt gtaacaaaat tttgaagtat
     1501 tttccgtata ttgcctgcta aacatgtcag atttgaaagg aaaatcatac tgtggtagaa
     1561 atcaattatg attatatggt agtacttata aataagtgat ttctattgaa tatgtaatga
     1621 agttcatata ttaatataac tttcattgat atagaagtcg caatgattaa tttaaacaat
     1681 aaaaaacaac catcatttcc aaactttgac aacgaatgat ggttaaagaa aatctcattt
     1741 agtcaccgtc tttttaacgg gctcaatagg cgacatgttc ctgcatatcg tctatttata
     1801 cactgcacaa tttaataata atacaattat actttgatgt ccatttaaca taaagtggtt
     1861 gaaatttgca gcaaataaat taaccttgac tattttcaga aaattctgta taaataacga
     1921 aaaagagata tggattaaat atgaagaggt gtttttatag aagaataaaa tttttggcac
     1981 tatattatat ttaactttag cacttggatt atcaacagca gcttatgcat ctacagaata
     2041 cgcagaagga ggcacttgga gtcatggtgt cggcagtaag tatgtttggt cgtattatta
     2101 tcatggtcat aaaggacatg gtgcaacagc tattggaaaa tatagatcat ttagtggtta
     2161 tacaagagct ggtgtaaaag caaaagcatc agctactaaa cataattggt gggtcaatag
     2221 agcgtattat aacatttatt aaatactaaa aaatcataga aaataaacaa cgcaagaggt
     2281 acaacatgaa atggttcaaa ttaatacttg atgttacaat cttcattctg attgctatat
     2341 tgttatttgt ttatacatat aaagaaaacg aagaaatatt gcctgatact aaatacccta
     2401 tagcggtaac tgactggaat aaaaaatata gtaaaaatga gatttataaa cgtatagatc
     2461 aattcgctaa aaatgagaac gtagcaatct ataaatcaac ttcaaattat acaaacaaaa
     2521 acgtagataa agatatatat gtatttaata aatcaaaagc agcaactatc actcctttta
     2581 atgctaaata taacattcat tatttaagtg acgacgaagt attaaaaaaa gatatcaaag
     2641 ggagttattt tgtaaaagac aaaaattttg acgtgtctaa attcataaat tttttaaaag
     2701 aatatggtgt tactgctgaa tcatacaaaa tagatcatat gatgattgcc gttggtgtca
     2761 ttaaacaaat gaatatagta gttccattat cctcactttt aatcgtttat tttatttatt
     2821 atattttcga aaagaacatt aatttcaaag cgtatgcgat taagtattta aatggtttta
     2881 cattaagaaa aataattttc gaaaattttt caaagaaatg cacgtattgg gtaacgttaa
     2941 tcataactca aattctttta actacaagtg tactttggat attaaattac acgggtaatt
     3001 tagatttatt catattaaga atagttctgc tttcatgtct tttcatttta acgattagtg
     3061 ttattaattt atggactttc ttaatgttac taaatttaaa tattgcaaat atgattaaag
     3121 gtaagcagca ctttaaaaca attcgtttta ttaatacagt ttgtaaaagt attctcttag
     3181 tactaatagc taatgtaatg atagaaaata ctagtgttat taaagatttg aataaaataa
     3241 aagaaactga gaaatattgg aatgtattag atgattatta cacgattgaa tttgcacctt
     3301 atcacgaaac aaaacaaagt ttgattgata atatggtgcg atcagaacaa ttagtaaagg
     3361 ctagtgaagc agaaaataat gcgattttat tcaaaccaaa gggtgactcc gttgacaatg
     3421 acaacttttc gcctgatgag gggaatgtaa tattagtaaa taatcagttc tggtcgattt
     3481 attacaagca gtttcaacct gatattccga taaaaaatca aaaaaataat gtcgaagtaa
     3541 ttattccaca aaagtttcat gcaatgcgta atgaaatcaa tcaagcatat cattcatggt
     3601 ttgaatttgt acaaaataaa aataataaag agaataagtt atctatacag tttatcaaca
     3661 aaaatgattg tcgaattttt tcatttgatg cacgagatag tcgccatttg tcatttatag
     3721 aggcgccaat cattgtgaat gttcaggcat cagatttatc gaatgatttt tattatgcca
     3781 tgatcagtca aggcgggtat ttattcaaaa attatgacgc gctagtaaaa aatattgaaa
     3841 agtatcatct tgatggggaa atcagtggaa taaccaatta taaagatagc gtgatggaaa
     3901 tgtatcatga aaacaatttg aaattaacag tactcaactt ttcacaaatc attatcgcaa
     3961 tcattttaat aattattatt ttatttgatg tgaaatatta ttttgaacag catcgaaaat
     4021 tactcgtaat caaaaagcta tatggttatt caacattaag agccaattac caatacttat
     4081 taataaataa tatagttgtt atttttattg gaatattgac gaatgtaatt ttacattctc
     4141 actatataat gatgttattt gcaacgattc ttgtcgttca aatattattg cagatttgca
     4201 gcctatacta tcatggccga cgttttaatg aagttatcaa ggagttttaa ctatgaaatt
     4261 tatcattgca atattattag gattaatatt atccattact attgctttta caatcataca
     4321 tcatcctata cttgatcgtt ttaatccttt cttaaaaacg gagtatagtt atgccaaagt
     4381 gccaaaaggt acgcaacaat atgttaatat tacggcttat agtgaaagag gggaaaagct
     4441 tgattataaa ttaacattta atggatt
//
LOCUS       DANRDI010000185         4442 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.187, whole genome shotgun sequence.
ACCESSION   DANRDI010000185 DANRDI010000000
VERSION     DANRDI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.187"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..148
                     /locus_tag="PZ377_002502"
     CDS             <1..148
                     /locus_tag="PZ377_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDM6359357.1"
                     /translation="PTKKAQSKKSELPETGGEESTNNGMLFGGLFSILGLALLRRNKK
                     NHKA"
     gene            328..1194
                     /gene="galU"
                     /locus_tag="PZ377_002503"
     CDS             328..1194
                     /gene="galU"
                     /locus_tag="PZ377_002503"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDM6359358.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(1405..2148)
                     /gene="sarU"
                     /locus_tag="PZ377_002504"
     CDS             complement(1405..2148)
                     /gene="sarU"
                     /locus_tag="PZ377_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HDM6359359.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSHADERIVL
                     IKINKIQYNTIKSIFTDTSKILKPRKFFF"
     gene            2477..2833
                     /gene="sarT"
                     /locus_tag="PZ377_002505"
     CDS             2477..2833
                     /gene="sarT"
                     /locus_tag="PZ377_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HDM6359360.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HNYIKNLLSEININET"
     gene            3139..>4442
                     /gene="sasG"
                     /locus_tag="PZ377_002506"
     CDS             3139..>4442
                     /gene="sasG"
                     /locus_tag="PZ377_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HDM6359361.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEP
                     SVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDF
                     SKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDT
                     GYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNT
                     SDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRW
                     GLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKLKRFHS"
BASE COUNT         1719 a          555 c          761 g         1407 t
ORIGIN      
        1 accaactaaa aaagcacaat ctaagaaatc tgaactacct gaaacaggtg gagaagaatc
       61 aacaaacaac ggcatgttgt tcggcggatt atttagcatt ttaggtttag cgttattacg
      121 cagaaataaa aagaatcaca aagcataatc aatccaaaat tgacaggttt atttcataaa
      181 ttatatgaag taagcctgtt ttttaaaatt aaaacaaatt tcccaagaaa taattacata
      241 ctcaatgaca ctatgaaggc gttctaatta gtgttaaaat gacgttgata catagattta
      301 atacttagga aaaggagcac attaactttg aaaaaaataa aaaaggcaat cattcccgct
      361 gctggtttag ggactagatt tttaccagca actaaagcga tgccaaagga aatgcttcct
      421 atcttagata aacccacaat acaatatatc gttgaagaag ctgcaagagc tggaattgaa
      481 gatattatta tagtgacagg tcgccacaaa cgcgcgattg aagatcattt tgatagtcaa
      541 aaagaattag aaatggtgtt aaaagaaaaa ggtaaatctg aattactaga gaaagttcag
      601 tattcaacgg aacttgcgaa tattttttat gtaaggcaga aagaacaaaa aggtttaggg
      661 catgcgatta gttctgcgcg tcaatttatc ggcaatgaac catttgcggt attattaggt
      721 gacgatattg ttgaatctga agtgccagca gtgaaacagt tgattgacgt ttatgaagaa
      781 acagggcact cagttatagg tgtccaagaa gttccggaag cagatactca tcgttatgga
      841 attattgacc cattaactaa aaacggacgt cagtatgaag tgaaaaagtt tgttgaaaag
      901 ccagcacaag gtacggcacc atcaaaccta gctattatgg gacgttatgt attaacgcct
      961 gaaattttcg attatttaaa aacgcaaaaa gaaggcgcag gtaacgaaat tcagttaaca
     1021 gatgcgattg aacgtatgaa taatgataac caagtgtatg catatgactt cgaaggcgaa
     1081 cgttatgacg ttggagaaaa attaggcttc gttaaaacta ccatagaata tgcattgaaa
     1141 gatgacagta tgcgagaaga attaacacga tttattaaag cattaggttt ataacattaa
     1201 aagcatgata ctcagtaaaa ttggaacacg aaatggtgaa ctaaattgaa tgagtgtcat
     1261 gcttaattta tgtgttgtga tatatagtag ggtattgaaa taatataaaa acaaaagaaa
     1321 gttaattatg tatttttatt tatactaaat tgagaagtag tatgattgct tatttatttt
     1381 atcaagaggt aaatctgttt aaatttaaaa gaaaaatttt cttggtttga gaatcttgga
     1441 agtatctgtg aatatgcttt taatagtgtt atattgtatt ttatttattt tgattaacac
     1501 aatcctttca tctgcatggg atcgttcttt gattagcata cctttattat ttaatctatt
     1561 aactgaccta acaatttgag gatacataaa tctaatactt tcaaacaaat cttttaaatt
     1621 tagtatctca ttttttcgag atgatattac atataagata gataaatcaa gaaatgttaa
     1681 attaaatcga tattttaaaa aatcttttga gtacataatg ttcataaaaa gtataaaaaa
     1741 ttctttgcta tattttggag cccattgata ttttatgtag tctctttcct tattaaatcc
     1801 tttaattatt ttatctaact cattaataac acaagcaatc tttttacgtt gtattggagt
     1861 aactgaaaca aaaatacggc gttcatctgc ttcgtttcta gacttattta taaatccttt
     1921 tttagatagt gacttaatgt tctttacaac atcagattgt ttgtaaagta tgtcaccaat
     1981 gatttctttt agtgaaatac tgtgttcatt tttgtaataa aaatatgcta atatcaacaa
     2041 ttcttttaat gataatttat attgttgctt taactcttga gtgagaaaaa atatgtacgc
     2101 ttctacattt atgaatttat tgaccttttc gaaagtttga tagtccacct tacattctcc
     2161 tactattttc tgatgtccca atactttata ttatttaaag taatataaaa gcaatctctt
     2221 ttaaagacat gttaacctaa atattttata tataatatat atatatataa aatataaata
     2281 actaaaaatc acagaataac aaatgacaaa ttcatttatt atccaaaaat aaaaacaatt
     2341 gaacaaaatc aaaatatagt gttatgattc atttgtgttt atagctagca ccacaaacta
     2401 aatatatttg gagttattac tagttaaaga caaatattat ttgacgtgac aattaaaagt
     2461 aagggatgaa gacttgatga atgatttgaa aagcaagagc aatattaaat taatgaaaag
     2521 agtattaact acatatgagc tgcgtaaata tttaaaaaaa tatttttgtc ttacattaga
     2581 taattattta gtattagcat atttagatgt atttaaaaat gatgaaggta aatattttat
     2641 gagagacatt ataagttata tcggtataga ccaatctaga attgttaaaa gcgtaaaaga
     2701 tttatcaaaa aagggttact tgaataaatg tagagaccca catgatagta gaaatgtaat
     2761 cattgttgtt tctgtaaaac aacacaatta tattaaaaat cttttgagtg aaataaatat
     2821 taatgaaaca taaagtatgt ttcgagattt taatatcttg gtgcagttaa atgtattttt
     2881 taaatgcgtt aaggtatttt ttattttaga aattcaatgc tattgagttg atgggttttt
     2941 ccaacagata agaatgaatt attcattgaa aactaaaaat aataatttta tttgtagatt
     3001 gttttaagtg cgtcaatata taaaataatt ttataaagat aagtgttata ttccattgtg
     3061 caactcctat tgtatttgta aaattacatg taaattcact gtaagcaaag tggaaaatat
     3121 ggaaaaagga gtatgcaaat gagagataag aaaggaccgg taaataaaag agtagatttt
     3181 ctatcaaata aattgaataa atattcaata agaaaattta cagttggaac agcatctatt
     3241 ttaattggct cactaatgta tttgggaact caacaagagg cagaagcagc tgaaaacaat
     3301 attgagaatc caactacatt aaaagataat gtccaatcaa aagaagtgaa gattgaagaa
     3361 gtaacaaaca aagacactgc accacagggt gtagaagcta aatctgaagt aacttcaaac
     3421 aaagacacaa tcgaacatga accatcagta aaagctgaag atatatcaaa aaaggaggat
     3481 acaccaaaag aagtagctga tgttgctgaa gttcagccga aatcgtcagt cactcataac
     3541 gcagagacac ctaaggttag aaaagctcgt tctgttgatg aaggctcttt tgatattaca
     3601 agagattcta aaaatgtagt tgaatctacc ccaattacaa ttcaaggtaa agaacatttt
     3661 gaaggttacg gaagtgttga tatacaaaaa aaaccaacag atttaggggt atcagaggta
     3721 accaggttta atgttggtaa tgaaagtaat ggtttgatag gagctttaca attaaaaaat
     3781 aaaatagatt ttagtaagga tttcaatttt aaagttagag tggcaaataa ccatcaatca
     3841 aataccacag gtgctgatgg ttgggggttc ttatttagta aaggaaatgc agaagaatat
     3901 ttaactaatg gtggaatcct tggggataaa ggtctggtaa attcaggcgg atttaaaatt
     3961 gatactggat acatttatac aagttccatg gacaaaactg aaaagcaagc tggacaaggt
     4021 tatagaggat acggagcttt tgtgaaaaat gacagttctg gtaattcaca aatggttgga
     4081 gaaaatattg ataaatcaaa aactaatttt ttaaactatg cggacaattc aactaataca
     4141 tcagatggaa agtttcatgg gcaacgttta aatgatgtca tcttaactta tgttgcttca
     4201 actggtaaaa tgagagcaga atatgctggt aaaacttggg agacttcaat aacagattta
     4261 ggtttatcta aaaatcaggc atataatttc ttaattacat ctagtcaaag atggggcctt
     4321 aatcaaggga taaatgcaaa tggctggatg agaactgact tgaaaggttc agagtttact
     4381 tttacaccag aagcgccaaa aacaataaca gaattagaaa aaaagttgaa gagattccat
     4441 tc
//
LOCUS       DANRDI010000186         4409 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.188, whole genome shotgun sequence.
ACCESSION   DANRDI010000186 DANRDI010000000
VERSION     DANRDI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.188"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(202..1548)
                     /gene="mtaB"
                     /locus_tag="PZ377_002507"
     CDS             complement(202..1548)
                     /gene="mtaB"
                     /locus_tag="PZ377_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDM6359362.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(1555..2307)
                     /locus_tag="PZ377_002508"
     CDS             complement(1555..2307)
                     /locus_tag="PZ377_002508"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDM6359363.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(2309..3247)
                     /gene="prmA"
                     /locus_tag="PZ377_002509"
     CDS             complement(2309..3247)
                     /gene="prmA"
                     /locus_tag="PZ377_002509"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDM6359364.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(3251..4390)
                     /gene="dnaJ"
                     /locus_tag="PZ377_002510"
     CDS             complement(3251..4390)
                     /gene="dnaJ"
                     /locus_tag="PZ377_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDM6359365.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
BASE COUNT         1300 a          910 c          585 g         1614 t
ORIGIN      
        1 atggtcaatt agtaaattgt tttttatttg aaacatattt cacatcaaaa tcacaaagac
       61 ttttagattt tgttctaaaa atctcttaat aatttattta atgagaagag ttgcttatat
      121 agtaaattgt gaagccgtta aaacaacgtt acaaaaccta tatctttaat acggaaccat
      181 atggtatgaa tcaaggaata cttaaactaa aacttctcta tcagatttat ttgttgcgaa
      241 atcaacaact ttaattgctt gcccttcatt taatggataa tttgcttgcg taattttaac
      301 ttttacaatt tgacctatga gtgattcgtc accttcaaat tgtactttca tataattatc
      361 tgcatatcca actaatgtac cttctgtgtc accctgttcc tcaggaatta cttcaagcac
      421 atcttgatca aatttagacg catataactt tccgagttga ttgcttagcg taattaactt
      481 atgcacccgt tcatttttaa tttcttcatc aatttggtca tccattcttg cagctggcgt
      541 gccaattcta ggagaataag ggaaaacatg cagttcagag aacttatgct ttacgataaa
      601 atcatatgtt tcttggaact cagcttcagt ttcacctggg aaaccaacaa ttacatcact
      661 cgtaactgcc aagtctggta aagctttatg caattttgtt aatcgttctg aaaatctatc
      721 cattgtatac ttacgtctca tacgttttaa tactgtatct gaaccagatt gtaatggaat
      781 atgcaaatga cgcacaactt ttgttgaacg ttctaaaacg tcaattactt catctgtaag
      841 ttgacttgct tcaattgaag aaattcgaat tcgttctaat ccattaatcg tttcaagatc
      901 acgtaataat tgggccaagt tataatcttt taaatcttga ccatatccac ctgtatgaat
      961 tcccgtcaat acaatttcct tatatcctga attcactagt tgcgtcgctt gttcaactac
     1021 tttttccgga tctcttgaac gcattaagcc acgagcccat ggaataatac agaatgtgca
     1081 gaagttgtta caaccttctt gaatttttaa tgacgcacgt gttctatctg taaaataagg
     1141 gacatctaat tcttcatatt tacgattttt catgatattt ccaacaccat taattggttg
     1201 gcgttcttta cggaattcgt caatgtaacc taatagttta tgtctatctt gtgtaccaac
     1261 tactacatcg acaccaggaa tttccataat ttcagctgat gaagtttgcg cataacaacc
     1321 tgttacacag attacagcat caggattttg tcttattgca cgtctaatta tttgacgact
     1381 ttttttatca cccgtattcg ttactgtaca agtattaata acaaatacat cagcattcgc
     1441 ttcaaagtca acgcgctcat agtttgcttc tttaaataat tgccagattg cttcagtttc
     1501 ataatggttt actttacaac ctaatgtgtg aaacgcaact gttgacataa atattcaccc
     1561 cattaattct ttttcataac ttattgcact taacgcatac aatggcgcag tttctgcccg
     1621 taaaattctc ggcccaagac caacaactgt actagtatta ctaaataatg aaatttcatt
     1681 ttctgacaaa ccaccctcag gaccaaaaat catcaacact ttatcctgag cattgaattg
     1741 ttgtaaagtt tgcttgaaat tgcttaactc accatctttt gcttcctctt catatgcaat
     1801 aagaatatag tcataattat caatagtatc acaaattaat tttaaattcg actcgaattg
     1861 aatagatgga atcactaaac gatagctttg ttcagcagct tctttaatta ttttttgcca
     1921 acgctctatc tttttggcaa cttttgcctc gtttaattta acaattgaac gttccatgct
     1981 cacagctata aatgatgaag cacccaattc agtagctttt tgtagcaacc actcatattt
     2041 gtcagctttg attagtccac tgcaaatcgt aacatcaact ggcaattctg tattaatatt
     2101 ttgtttttct tttaaatcaa cttcaatttt atcacttgtt atgtcagcaa tttcacataa
     2161 ataaactgtt tgatcattaa aagttaaaat aattttacta ccaacatcat atctcattac
     2221 atttgttata tgatgaatat cttctttttt tgtaataaaa aaacgctgac ttacatcagc
     2281 gttttggtct atgaaataac gttgcacatt attcactcac tttctggcca acaagacaaa
     2341 cccaaccgtt gtcatgttgt tctgaaataa ttttaaaacc tacacgctcc atatgtgact
     2401 gtataccttc atacttctct tttataatac cagaagtaat aaaataaccg ccttcattta
     2461 gagtattata agcatcttca atcatttcat caataatatg cgctaaaata tttgctatta
     2521 caatatcaaa tttttctgtt tcgtctttca ataagttacc tggaacagct tcaattaacg
     2581 tttcacaatg atttcttctg aagttttctt tagctacact cactgccatt tcatcaatat
     2641 ccaacgcttt aatacgtttt acaccgatta gatgacttgc aatacttaat atacctgagc
     2701 cagtaccaac atcaattact gaatgctgtg gcaatacata tgtttctatt gccttcaaac
     2761 acatacttgt agtcggatga tcacctgttc caaaagccat acctgggtcg agctcaatgc
     2821 aaagctcttc atccgcttct ttagcatatg tttcccaact aggaactatt gtgaacttct
     2881 tcgacgctcg gaatggatgg aaatagtttt tccattcatt ttcccaatcc gtctctgcaa
     2941 taatttgctc actgaattga acgttatgtt gatcaagttc atctaaattt aataactcat
     3001 ctttaatttg ctgtcgcaac ttatcatcat aagtcatttc attaaaatag gctttcaatc
     3061 ttactccctt atctggataa tcctcttttt tcaaagcgta aatttcaccg tatttatctt
     3121 ctggttggtt aattaaatca tctgaatctt ctatcacgac accatttgat ccatgatttt
     3181 caagtatatt ggtagccaat tctactgctt catgattaat aataattgaa agctctgtcc
     3241 agttcatact ttattctccc ttaaagaatc tttttgctct atctttaaaa ttcgaaggtt
     3301 gttcattaat ttcttcacca tttaattggg caaattcttt cattagttct ttttgtctat
     3361 ctgttaattt agtaggcgtt actactttaa tatcaacata taaatctccg tatccatagc
     3421 catgaacatt ttttataccc ttttctttta agcggaattg cttacctgtt tgtgtaccag
     3481 cagggattgt taacataact tcattattta atgttggtat ttttatttca tcgcctaaag
     3541 ctgcttgtgg gaagctaaca tttaatttgt aataaatatc atcaccatca cgtttaaatg
     3601 tttcagatgg tttaactcta aatactacgt ataaatcacc agcaggtcct ccattcacgc
     3661 ctggagagcc ttcaccagct aatctaattt gttgttcatt gtcgacacct tcaggtactt
     3721 tcacttctaa tttaactgtt ttattttcag taccttttcc gtgacatgtt ggacaagctt
     3781 cttcaaattc ttgaccactt ccattacatt taggacaaac ttgttcagta cgaactctac
     3841 ctaaaattgt gttttgttct acagctacat gaccagcgcc attacagtaa ctacaagtct
     3901 ttttacttgt tccaggcttt gcaccatcac catgacatgt ttcgcatgtt acatctttac
     3961 ggattgaaat ttcttttgtt gtaccaaata ccgcttcttc aaatgttaat gtcattgtat
     4021 actgaagatc atcacctttt tgcggtgcat ttggatctct ttgtctgccg ccaccgaaga
     4081 aagagctaaa gatatcttca aaaccgccgc caccgaagcc actaaaaccg ccaaagtcag
     4141 agccattgaa tccttgtcca ccaaaacctt gtggaccatc atgtccaaat tgatcatagc
     4201 ttgcgcgttt attatcatca cttaaaactt cataggcttc agaaatttct ttaaactttt
     4261 catctgcacc ttcttctttg ttaatatctg gatgatattt tttcgaaagc tttcgatacg
     4321 cttttttgat ttcatctttt gaagcatcct tactaatgcc taaaacttca taataatctc
     4381 ttttggccac agctatctct ccttttctt
//
LOCUS       DANRDI010000187         4321 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.189, whole genome shotgun sequence.
ACCESSION   DANRDI010000187 DANRDI010000000
VERSION     DANRDI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.189"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            48..1805
                     /gene="sfaB"
                     /locus_tag="PZ377_002511"
     CDS             48..1805
                     /gene="sfaB"
                     /locus_tag="PZ377_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDM6359366.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            1812..2882
                     /gene="sfaC"
                     /locus_tag="PZ377_002512"
     CDS             1812..2882
                     /gene="sfaC"
                     /locus_tag="PZ377_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDM6359367.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(2983..3108)
                     /locus_tag="PZ377_002513"
     CDS             complement(2983..3108)
                     /locus_tag="PZ377_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359368.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            3274..4257
                     /locus_tag="PZ377_002514"
     CDS             3274..4257
                     /locus_tag="PZ377_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDM6359369.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
BASE COUNT         1635 a          569 c          782 g         1335 t
ORIGIN      
        1 ctattttata aatagaaagt tgaagttaaa gttggaggaa agtaatcatg gtatatcttg
       61 aatgggcaaa ggcagataga aatattcaat atcgtgtaat taacgccatt attaaagaac
      121 gtatttaccc cgagcaaaca tttatttcgc aaaaaggatc tttaattgaa attcagtatc
      181 atatgcatgt gttgactatt gaagttgtta gaaaaagtgc attagaacgc tatgagttta
      241 caggtgatat tacttattta aataaaggtg aaacgtcatt aattataact ttagaaggtt
      301 tattagatgt gttgaatcat gactttgata tccctatttc agagcgacta cgcgaagagt
      361 taatacacag tcgagatagt ttagttgaaa catataagca aatgtctcac agacaaacgt
      421 taataagtca aagttttaaa ttttcaaggt taccacaaga tattaacttt ttttcatggt
      481 tacaacatgt aaaagatagt gataagacag atgatttaac ttattctgag agtttggtac
      541 cagaggggca tccaacacac cctttaacca aaacgaaatt gcccttaact atggaagaag
      601 tacgagcata tgcacctgag tttgaaaaag aaatcccttt gcaaattatg atgattgaaa
      661 aagaccatgt tgtgtgcaca gctatggatg gtaatgatca atttattatt gatgaaataa
      721 ttcccgaata ctacaatcag attcgtgtgt ttttaaagag tttaggtttg aaaagtgaag
      781 actatagagc gattttagta catccttggc aatatgatca tacgataggg aaatattttg
      841 aagcatggat tgctaaaaaa atattaattc caacgccgtt tacaatactt tcaaaagcaa
      901 ctttatcatt taggacgatg tctttaattg ataaaccata ccatgttaag ttgcccgtcg
      961 atgcacaagc aacaagtgcc gttagaacag tctcaactgt gactactgta gatggaccaa
     1021 agttaagtta tgctttacaa aacatgttga atcaatatcc aggatttaaa gttgctatgg
     1081 aaccgttcgg tgaatatgca aatgttgata aagatagggc acgtcagctt gcttgtatta
     1141 taagacaaaa gcctgaaatt gatggaaaag gtgcaacagt agttagtgca agtctagtta
     1201 ataaaaatcc aatagatcaa aaagttatcg tggatagtta cttagagtgg ttaaatcaag
     1261 gaattactaa agaaagtatt acgacattta ttgaacgata cgctcaagca ttaatcccgc
     1321 ctttaattgc ttttattcaa aattatggaa ttgctttaga agcacacatg caaaatacag
     1381 tagtgaactt ggggccacat tttgacattc aatttttagt gagagattta ggtggttcta
     1441 gaattgattt agaaacatta caacatcgtg tatcagatat taaaattaca aatgatagtt
     1501 taatagctga ttctatagat gcagtgattg caaaattcca acatgctgtt attcaaaatc
     1561 aaatggcaga attaatccat cattttaatc agtatgattg tgttgaagaa accgaattat
     1621 ttaacatagt acagcaagta gtagcgcatg ccattaaccc aacactacca catgcaaatg
     1681 agttaaaaga tattttgttt ggaccaacaa ttactgtcaa agcgttgtta aatatgagaa
     1741 tggaaaataa agtaaagcaa tatttaaata ttgagttaga taatccgata aaaaaagagg
     1801 tgtagtacta catggcacac gttaacataa atatatcgaa gattaaatat aacgccaaag
     1861 tacttcaaac agtttttcaa agtaaaaata tgcaattcac accagtaatt aagtgcatag
     1921 ctggtgaccg tacaattgta gaaagcttaa aagcgttagg tatcaatcat gttgcagaat
     1981 ccagattgga taacataatt agtattgcag atcaggattt aacatatacg ttattgcgta
     2041 cgcctgccaa aaaagagatt tcagatatga tagaaaaagt tgatatgagt atccaaacag
     2101 aattatctac catacatcaa ataaatgaag tagcagaagt tttaggaaaa aaacataaga
     2161 ttttattaat ggtagattgg aaagatggcc gtgaaggtgt tctgacttat gatgttttag
     2221 attatattaa agagattatt cacttgaaaa atatccattt tgtaggttta gcattcaatt
     2281 ttatgtgttt taaatctgat gcgccaagtg atgatgatat ctttatgatt aatcgattcg
     2341 tctcagctgt tgaaagagaa ataggatatc gattgaaaat tatttcaggt ggtaattcga
     2401 gcatgttacc acaactttta tataatgatt tgggtaaaat aaatgaattg cgaataggtg
     2461 aaacgttgtt tagaggtgtt gatacaacga caaatcaagc gatcgctatg ttatatcaag
     2521 atgcgattac attggaggct gagattttag aaattaaacc tcgagttaat acacaaacgc
     2581 atgaatcatt cttacaagct attgtagata ttggttattt agatacaaaa gtcgataata
     2641 tttcacctat ggatcagcat atcaatattt taggcgcttc gagtgatcat ttaatgcttg
     2701 atttaaatgg tcaaggacac tatcaagttg gagatcatat atctttttca ttaaattatg
     2761 aagcactttc tcattcgatg tatatgaaaa atttacataa ggtatatatt gatgattcaa
     2821 agattgatac gctacttcaa aattttgatg tgaaatctcc tgctatggta aaccactatt
     2881 aatatattta tcaataagtc taagttgaca agttatacta catatcgttt gcacggttgt
     2941 atcataattg ttcaacttag attttttgta tttgttgatt tatcaaatta agtgcaacag
     3001 ttcgtcacat aaaattgcaa cagataatat cagctgaatt acagggataa cggtcatgct
     3061 aaatggtgtc aattgtatta atgcaaagat gatatagcaa tgatacatta tcagtatttt
     3121 gtctaaggaa atgtgctaat tgtagtcata attattagag gaaatatata gtcatacatt
     3181 ttagaaatac aaaaaagatt gaacgctact tgacaatgat aattgttatc aataaaataa
     3241 taaatgaagt tatacatatt aaggagtgga acgatgagag gtctaaaaac ttttagtata
     3301 ttgggattaa tagttgcctt acttttagtt gcagcttgtg gtaatacgga taattcaagt
     3361 aaaaaagaat catcaactaa agatactatt tcggtaaaag atgaaaatgg tacagtaaaa
     3421 gtacctaaag atgcaaaacg tatcgttgta ttagagtact catttgcaga tgcattagca
     3481 gcattagacg ttaaaccagt tggtattgct gatgatggta agaaaaaacg tatcattaaa
     3541 ccagttagag aaaaaattgg ggattatact tctgtaggta cacgtaaaca gccaaactta
     3601 gaagaaatta gtaaattaaa accggattta attatcgctg atagcagtag acataaaggt
     3661 attaataaag aattaaacaa aattgcacca acattatcat taaagagttt tgatggagac
     3721 tacaaacaaa atattaattc gttcaaaaca attgctaaag ctttaaataa agaaaaagaa
     3781 ggcgaaaaac gtcttgctga gcatgataaa ttaatcaata agtataaaga tgaaattaaa
     3841 tttgatagaa atcaaaaagt gcttccagca gtagttgcta aagctggttt attagcacat
     3901 ccaaactatt catatgttgg acaattttta aacgaactag gatttaaaaa tgcattaagt
     3961 gacgatgtaa caaaaggttt aagtaaatat ttgaaaggac cttacttaca attagacact
     4021 gaacatttag ctgatttaaa tccagagcgt atgatcatta tgacagatca tgctaaaaaa
     4081 gattctgctg aattcaagaa gttacaagaa gatgcaacat ggaaaaagtt gaatgcagtt
     4141 aaaaataatc gcgtggatat tgttgaccgt gatgtttggg caagatctcg tggcttaatt
     4201 tcttctgaag aaatggctaa agaacttgtt gaattatcaa aaaaagaaca aaagtaaggt
     4261 ggaagtaaat ggctataaaa gaaataagta gccaatctgc catagatcat aaaagaaaaa
     4321 g
//
LOCUS       DANRDI010000188         4316 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.190, whole genome shotgun sequence.
ACCESSION   DANRDI010000188 DANRDI010000000
VERSION     DANRDI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.190"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1241)
                     /locus_tag="PZ377_002515"
     CDS             complement(<1..1241)
                     /locus_tag="PZ377_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359370.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYA"
     gene            complement(1256..1570)
                     /locus_tag="PZ377_002516"
     CDS             complement(1256..1570)
                     /locus_tag="PZ377_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HDM6359371.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(1548..2366)
                     /gene="recX"
                     /locus_tag="PZ377_002517"
     CDS             complement(1548..2366)
                     /gene="recX"
                     /locus_tag="PZ377_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDM6359372.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(2627..3436)
                     /gene="sgtB"
                     /locus_tag="PZ377_002518"
     CDS             complement(2627..3436)
                     /gene="sgtB"
                     /locus_tag="PZ377_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDM6359373.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(3777..4292)
                     /locus_tag="PZ377_002519"
     CDS             complement(3777..4292)
                     /locus_tag="PZ377_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDM6359374.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
BASE COUNT         1310 a          766 c          538 g         1702 t
ORIGIN      
        1 gcatagtaat tactataatt tgatttcata tatggtgcta attttttaaa actatgcgtt
       61 tgatataaac ctgccgggtt aaaatattct aatttatctt tactaacata gtaagtctta
      121 ttatcggcgg taacacgata atttttacta ttttcgccag taattgtaac tacactatat
      181 tttggaattg tgacttgttt atccccttgc atatccacac tgccattaaa agcaattcca
      241 taagctaact tttcgccata tggatcttta ttttcatttt gcatcgtagc acgattcgta
      301 aaatctgtta tcgaaataat tcctatatta ttgaaaatga gcaacatcat caatgctaag
      361 cctaaaatag tacctgaggc caaagtccaa aaacgcacta aaaacttagg cggttttgca
      421 tgattatagc gctcaacacg tttgaaatta taggtcatct ctggtattct tgtacgattc
      481 tttttccaat ctaaatcaaa gttttcaatt tcttcttttg agtttagact taatcgatca
      541 cgttcatgtt ctttaaaaga tggaataact tgtttaagtg acccttccat tcttaattga
      601 ccatgtgaaa accaagctat gtagttactc acttgtgaaa ttttatcaat atcatcacca
      661 attgacacaa tcgttaaatt attttcaata taatcatttg ttaattcaat cgcacgttcc
      721 ataaattgtg gtgttaaata gtcaataaca tgatttaaaa taataatatt tgattttgat
      781 gagcgtgcaa tacttaatag taattgagcg tatgccgctt tcgaaatatg gttaaccggc
      841 ttcgttttat aatcacctaa atgtgcatat tgaataatct gttcagcttt atgatcatta
      901 atttcatatg gaaatagttg aactaactgc gctgtataag cttcaacagt ttgatgaatt
      961 aacgattgat cttcaatata tccgtagaac aaatcttcag tacaaactac tttaccttta
     1021 tcaggtttaa ttgcacctgc caacaattga cctaccaacg ctttggaaga ttcaggttca
     1081 ccaattatac ctaatgcttc tccttgataa atatgtaaac taatattgtt taaatcgata
     1141 tcttcagcat catatccaaa aggtaaatac catttcttat tctgtttatt cctatagtag
     1201 tgtgttactt ttagtaactt taaaacaatt gaacttccca tctattttca tccttctata
     1261 attgtaataa cgctataggt aatccttctt cgggttcatc gctattaaat ctaaaccccc
     1321 acgcaaaaat accttttaat ctttctactt taaaatattg attactgcca tcagtcagtt
     1381 tataaatttt accaatttct atcttttttc tgtctactaa gtaactttcg gcaacaatta
     1441 ctttactttg ataaacgtca tattcattta aaataccatt catctcagct tttctcattt
     1501 tctctttata aagttgtatt tcgtgtctta attctggctc agacatctca ctcaatttct
     1561 tctgttccat cggcaatacc actttcttct aatttagctt taattttatc atatttatat
     1621 ccttttctca taaggccttc gatagttttt gaaattaatt tctgttgcgt gtacttcttt
     1681 cgatttttat tataaatttt ttctaaatct cgttgtaata aatcgtctaa aaccgcttca
     1741 tcttgtgtaa aatccatttc attcaataca gcatgaatgg tttccatttc aaaccctttt
     1801 tgaattaaag attgcattac tttttcttta actttatttt gtggcccttt ttttgtttta
     1861 gatatttttt cagcaatttg gataatatca tcaagttcct gttgttctct ataaagttct
     1921 gtaaatattt caatgatatt tggttctata ccaagttgat atagtttttg ttgataaatt
     1981 ttaggtcctt tatctgtcgt gcgaatcatt gtatttttta aactttccgc ataatcttga
     2041 tggtcgatta acttttcgcg ataacaatat tcaatcactt cagaaatcgc ttgctctgat
     2101 atctcttctt tttgtaaata ttgtataact tctttttcag ttctcttttt atatgataaa
     2161 tattggattg ctttatttaa acctatgcga taatgatcat acttttgaat ctctgccata
     2221 tcagcagctt caagttgttg cccttttttt aaattaaatt tgactaatgt atcaatatct
     2281 atacccattt caaattgttc gtctaaaaaa agattaaacc gttctttgtt tttcttttga
     2341 acttctattt tagtaatctt cggcactgga tcaccccgtt actttcttaa gttttaatac
     2401 aatactcttt tattattcaa taagccactt cctatagcaa atgtttaact ttaaatattt
     2461 ttcgatgcta acaaaaaatc acactatcat cttttaaaat gaaagtgtga ttacaagcaa
     2521 atctgtaaaa tttataaagc agaaacaatt caactttatc attatgacat ttcaattaaa
     2581 ccttctacat tatagttcca agcatcttac acatgaatgc aagtatttaa cgatttaatt
     2641 gtgacatagc ctgttgatat tgtgtttcat tgatataatt ttgttgcttc attttttcta
     2701 agttcgtgct tacacgttgc gtaaaattct ctgacatatt attgatatta tatacgctag
     2761 gtgcattgac tttactagct aaaatagcgc tttgtaaaac tgttatgtga gacattgttg
     2821 tactattttt attcacggtt gttccaaagt aatggtttgc tgcgccctca agcgtatatt
     2881 gattatcccc aaagtaaata ttatttaaat aaaagcttaa aatttcgttc ttattatatt
     2941 gtttttcaac tcgatgagct acaaataatt cttttacttt tctagtaaat gaacgatcat
     3001 tatcataaaa ataatttttg acaacttgtt gtgtaatggt actaccacct tgcacatctc
     3061 tgtcgctaat cgttgaaaat aaagctctag ttgtaccttt caaatcgaat ccatgatgat
     3121 tgtagaatcg ttcatcttcc attgaaataa aggcaccttt aacatactct ggcatgttat
     3181 cagctgacac aaaactactt ttattttcaa tttttcttag ttcatccaca ttatcgcgtg
     3241 tagataaaaa atacatgata ccaataaaca atgcgataat gattagaatg gttaataata
     3301 tttttaatag tattcgttta ctttttttct ttttcggcgg tttgccaact ggttgataat
     3361 acgtattata gtgaggttcg tgtttcatat gctcaaaatg ttcatttgag tttgagtacc
     3421 tatcgcttct tttcatgcgt ttgctccttc ttttaaaact cacttagtat ataccttgag
     3481 tttaccagta ctatcacaaa taggctacac tttttgggaa aatcagtcca agggcttaca
     3541 atcgtatacg ccatcatact tacttttttg ttttttgaaa aaattataga taaatcattg
     3601 caattttaaa tattaatcat gtcaaatatt gttatatttt ataaaaataa aagaccatcc
     3661 ctattaaatg ccaatagaga cgacctttta tttgttattc atttattaaa actaaaatcc
     3721 atatttcatt tcaaacgaaa atatataaat tttaacaatc gataaccaca atacttctat
     3781 tgtaattgtt taacgatttc tcgattaaaa tcatctaaat cgtctggtac tcgacttgtt
     3841 acaatattgt tgtctacaac tactgactca tcaactacat gtgcgcctgc atttgataaa
     3901 tctttgcgta catttaatac tgctgttaac gtacgacctt ttaaatcgtc tgtatctatt
     3961 agtatttgtg gcccatgaca aatggcaaat gttggtacat catttttagt aaagtattta
     4021 gcaaatgtgc catatcgacc ttctgtatct ccacgtaaat gatctggtga aaatcctcca
     4081 ggaattaata atgcatcata atcttctggt ttagcttctg caatgcctac atcgacagta
     4141 actttttcac cgtgtttacc aacaacttca ctatttgcag tatctccaat cactacagta
     4201 ttaaagcctg cattctctaa tgcctcttta gggcttgaat attctatatc ttcaaattcg
     4261 tttgctagaa taattgctac ttttttagtc attgcaaatc acctttctat atatca
//
LOCUS       DANRDI010000189         4281 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.191, whole genome shotgun sequence.
ACCESSION   DANRDI010000189 DANRDI010000000
VERSION     DANRDI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.191"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..841
                     /locus_tag="PZ377_002520"
     CDS             <1..841
                     /locus_tag="PZ377_002520"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDM6359375.1"
                     /translation="LAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDLWYGE
                     NHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRDQDIF
                     DQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSLRGAA
                     IWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRVEELV
                     QELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTEDYISG
                     RFG"
     gene            848..1489
                     /gene="phoU"
                     /locus_tag="PZ377_002521"
     CDS             848..1489
                     /gene="phoU"
                     /locus_tag="PZ377_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDM6359376.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(1629..3443)
                     /gene="pepF"
                     /locus_tag="PZ377_002522"
     CDS             complement(1629..3443)
                     /gene="pepF"
                     /locus_tag="PZ377_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDM6359377.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            3648..3992
                     /locus_tag="PZ377_002523"
     CDS             3648..3992
                     /locus_tag="PZ377_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359378.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            3940..4125
                     /locus_tag="PZ377_002524"
     CDS             3940..4125
                     /locus_tag="PZ377_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359379.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
BASE COUNT         1481 a          660 c          675 g         1465 t
ORIGIN      
        1 acttgcacaa actaaacaaa tatctcaaag tcatacgttt gatgtctcac aaagtcatca
       61 taaaacacca gatgatacaa actcacattc tgttatatat tcaacacaaa atttagactt
      121 atggtatggc gaaaatcatg cattacaaaa tattaattta gatatttatg aaaaccaaat
      181 tactgccatt ataggtccat ctggttgtgg caaatcaaca tatattaaaa ctttgaatcg
      241 aatggttgag ttagtacctt ctgtgaaaac agctggtaaa atattatatc gagatcaaga
      301 catttttgat caaaaatatt ctaaagaaca attacgtaca aatgtgggca tggtctttca
      361 acaacctaat ccatttccaa aatcaatata cgataatatt acttacggtc caaagattca
      421 cggtattaaa aataaaaaag ttcttgatga aatcgttgag aaatcattac gtggcgctgc
      481 aatttgggat gaattaaagg ataggttgca cacaaatgca tatagtttat ccggtgggca
      541 acaacaacgt gtttgtatcg cgcgttgttt agcaattgaa cctgaagtca ttttaatgga
      601 tgaaccgaca tcagcattag atccaatctc aacattaaga gtagaagagt tggttcaaga
      661 actaaaagaa aagtatacaa ttattatggt tacacataat atgcaacaag cagctcgtgt
      721 atcagataaa actgcatttt tcttaaatgg ttatgtcaat gaatatgatg atactgataa
      781 aattttctct aacccatcaa acaagaaaac agaagattat atttcaggaa ggtttggttg
      841 atatataatg gcaataatta gacaacgata tcaggagcaa cttgatgatt taataaaaga
      901 attacgtcgg ttaggtgcaa atgtctatgt gagtattgaa aatggtataa aatcattaag
      961 tattgacgat agaggctttg cacgacaaac agttaaaaac gataaacata tcaatcaatt
     1021 aaattatgat attaatgagc gagttatcat gttaattaca aagcaacagc ccattgcgag
     1081 tgatttgcgt atgatgattt cttcattaaa aatcgcctcc gatttagaaa gaataggaga
     1141 taatgcctcg agtattgcca atattcgatt gcgtacaaag attacagatg attatgtgtt
     1201 aacccgttta aagacaatgg gtaaattagc tatgttaatg ttaaaggact tagatcaagc
     1261 atttaaaaag aaagataccg tattaataag agaaataatt gagcgtgatg aagatatcga
     1321 tgacttatat agtcatatta ttaacgcaac gtatcttatt gataacgatc catttgtcgc
     1381 tgcacaagct catttagcag caagacattt agaacgtatt ggtgatcata ttattaacat
     1441 cgctgaaagt gtttattttt atttaacagg tacacattac gaacaataac ttaaagttat
     1501 tactataaaa tcccttacga taaatatata tttctattat tcataaaccc tcaaaaaaac
     1561 caagattctc acaattagta atgtgaaaat cttggtttat attgttctac tataaattgt
     1621 ctcgcatctt agttatttgc ttgctcaatt tcatctgtta atttttcaac ttcatcgact
     1681 aaatcagaaa tatattgaat tgtagattta agtggctgtt ctgtagtaat gtctacacct
     1741 gcaatgtttg caagttcgac aggtgataca ctaccacctt ttttcaatgt ttctaaccaa
     1801 gcatcaacag ctggttggcc ttcattttta atcttttgag aaacgacagt tccgattgtt
     1861 aagccagcag aatacgtata cgaatataat cccatatagt aatgaggttg acgcatccat
     1921 gttaattcag caccctcagt catgtctact gcatctccaa aaaattgttt ataaacattt
     1981 agcattattt catttaatgt cggcgcattt aaagattcac cttgatctac tttgtgatac
     2041 acttcacgtt gataagcagc ttctaataaa tgggtaacca tattatgata atatgttcta
     2101 gataaaattg agccaataac ccaacgctta aatcttggat tatcacttgt gttaaataaa
     2161 taattggcca tcaacatttc attcattgta gaaggggctt caacaaagta cattgatgct
     2221 tctgattcaa gatatggttg atgtttttga gctaatgtaa aatgacctgc atgacctaat
     2281 tcatgtgcta agacaaatgc ttcagccatt ttaccagtcc aagaaataaa cacatatgaa
     2341 tgtgtaaagt atggacttgc acaaaatgcg cctgtatctt tacctttatt ttgtgcaaaa
     2401 tcaatccatc gctgatcgta tgcttcacgt aacatgtttg tatagtcatc acctaaaaca
     2461 cttaacgcac cgaaaatata attttttgag tcttcaattg aaatctctgg ttcataatca
     2521 gggtctacag aaatcttcaa gtcttcaaaa cgcatgttat ctaatccgtg aatacgttgt
     2581 aaaagtttag catatttctg cataactggt gccaagtcac gcataatcat atcgatttga
     2641 cggtcaaaca tatcacgcgt tacttcttga ctatgtaata aataatcgat gactgattca
     2701 aatccacgta aatcagcttc aattttttct tgttgtactt gcatattata tgtagccgcg
     2761 gtagtatgct gatattttcg aatcccatcg ctaaacgatt tgaaactttt acgtctaaac
     2821 tcaggatctt tattatcttc ataatcattt tcaaacgtag catagtcgac agggtacgtt
     2881 gtaccatcat gttcaaatga atcgaatgta atatctagca tttttgtcgt gccgtataaa
     2941 tcgtaaggac tgtttagcgt aggtgataaa gttgctaata cttgttctac cgacgcagat
     3001 aattggaatg gcttttgttt tattaactgt ttaatatagt gttgatatgg acatgattct
     3061 tctaattgtt gaagtatttc ttctggtaat tcaagtattt ctgactctac aaatgataat
     3121 tggctaacaa ttttaccgta tgtagtagat aatttagcgc tcaatacttg tgcctcgata
     3181 ttactagtat ctacacttaa acgtagttct gcataattac ttaagcgatc taaggcaatt
     3241 aaaatatttt ctaattcagc taaagcagta ttaatttgtt cgattgaatt taatgttgtt
     3301 gcatatgtat gatgaaattg atttgcttgt tgtactagag cgtcaatact ttcataatat
     3361 tgttgatcat ctttaaataa gtctactaaa tcccatgttt ctgaaacagg aacatcttct
     3421 cttaaaggta aaccttgaga catataatca ctcctttggt ataatacatt cattatacat
     3481 aattttgtct cctaaacgat aatgttacga tttccatgtc gcaaggaaca atgatttttc
     3541 tattagtaga tagaatattg cttgaaactg actgctattg tttaagtttt taaaaattta
     3601 taataaaatg tatacaagga aagtaatgaa ataaaaggag aatgaatatg acattattcg
     3661 atatgcctaa ttatttatgg atcacaacat taataatgat tttattaaca atattctgtt
     3721 gcttagtttt aaataaatgg tttgtatctg cagtaattac atttgttata ttaggtgtgc
     3781 ttgcattttt tattccaaat tttcaagata taaaatatca accattatta ggatacgctg
     3841 catttttagc gattatgagt ttattaatta gttttctatt atggtatttt actagaaatt
     3901 ggcgtaaaga aagaaaagcg cgtaaattag aaaagcaaat tgaaaaatac ggctatgagg
     3961 gcgctgaact tcgccgtaaa gataaaaaat aatattcgac aactatatgc acaaaatcat
     4021 cgacttttaa gttacactgc agtaacaatt atttcgaaag agatggaata cagtgctata
     4081 gttgtttttg ttttgacttt tttatatttt acatggttga cttgattcaa aatattatat
     4141 tttataatga ttttgttaaa aaattaaata tacttcaatt tctttatgga taatactata
     4201 atgaacgtta gttcttaata tgtaagttgc atatacatgc gccattctca tgtaaaaaga
     4261 aagtgttagt aacttacaca a
//
LOCUS       DANRDI010000190         4205 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.192, whole genome shotgun sequence.
ACCESSION   DANRDI010000190 DANRDI010000000
VERSION     DANRDI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.192"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1175)
                     /locus_tag="PZ377_002525"
     CDS             complement(<1..1175)
                     /locus_tag="PZ377_002525"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDM6359380.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDF"
     gene            complement(1193..2074)
                     /locus_tag="PZ377_002526"
     CDS             complement(1193..2074)
                     /locus_tag="PZ377_002526"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDM6359381.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(2102..3409)
                     /locus_tag="PZ377_002527"
     CDS             complement(2102..3409)
                     /locus_tag="PZ377_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDM6359382.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(3627..4154)
                     /gene="pyrR"
                     /locus_tag="PZ377_002528"
     CDS             complement(3627..4154)
                     /gene="pyrR"
                     /locus_tag="PZ377_002528"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDM6359383.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
BASE COUNT         1296 a          826 c          623 g         1460 t
ORIGIN      
        1 aagaaatctt ctcctttaat ttcttgttca ctatctaaat caatgattgt taaatctgca
       61 taaccatttt cttttaatgt gccgtattct aaattaaatg tctcacatgg tttaattgtt
      121 aagtaatcta ctaattgttg taatgtccaa tcaccatttt ttacaaaatg cgtatataat
      181 aatgggaatg ctgtttcact accaacaatt ccgaatggtg ctttttccat tggttgtgct
      241 ttttcatcac gtgcatgtgg tgcatggtct gttgcgatac agtcaattgt accgtctagt
      301 aacccttcta acaaagcctc tctatcttca gtacttctca atggtggatt cattttataa
      361 atggcattat taccaggaat atcatcttct gttaacaata aatggtgtgg tgtaacttca
      421 gctgtaacat gaatgcctgc gcgtttagcg tcacgaatga ctctaacact ttctttagta
      481 gaaacatgac atacatgata atgacaacct gctgcttcag ctaatagtac atctcttgcg
      541 atttgaacag attcacaaat gtttggaata cctggtatac ctaactcttt actgcgtttc
      601 ccttcatgca ttgcaccacc atagattaat gaattatctt cacagtgtgc tacgatggct
      661 ttgtttactt ttgcagcttc aatcatgcct tcatacatca tgcttgcagt ttgtactcct
      721 acaccgtcat ctgtaaacgc aaaggcacct tcttttacta gtgctgggaa atcaaccaat
      781 tctttaccta attgacgtgt tgtaattgaa gcataaggta atacacgtac ttgagcattg
      841 tcatcgatta atttttgtaa agcttcaaaa tgttctacag aatctggtac cggtcttgtg
      901 ttaggcattg gacatacagt tgtaaatccg cctctagcag cagctttagt accagtttca
      961 attgtctctt tatattcacc accaggttca cgtaaatgaa catgaacatc gacaaatcca
     1021 ggtgacacaa agtgaccttt cgcatctatg atgtcaacac cattgcttgg ttcaattgca
     1081 ggtgcaattt gtttaattac cttaccatca attaaaatat ctgcttgttg taattcgcca
     1141 ttttgtaata ctttaccgtt tttaattaat ttcatcattt tcgtcccctt acctatttta
     1201 ataattcatc aatgactgcc attcttaagt aaacgccatt ttccatttgc ttaaaaattc
     1261 ttgattttga agcttctact aaatcgcttt gtatttctac tcctctattc acaggtgccg
     1321 gatgcataac aatagcatgt tcttgtaatt tgttatagcg tacttcattt aagccatgct
     1381 tttgatgata atcatctgct gcaaatctag tttcttctgc aagcccatgc ctttcatgtt
     1441 gaattcttaa taacataact atatctactg tttctataac atcatctata tttacataag
     1501 gtgcttctaa agaatcatca atccaagcat ttgggctatt aaacattacg tttgcaccta
     1561 atgcttttaa actatggtaa ttactacgtg cgacacgtga atttttaatg tctccacaaa
     1621 tcaatacatt caagccttca aaatatccat attcttcata tatcgtcatt aaatcaagta
     1681 aactttgtgt tggatgttgt ccactaccat caccagcatt cgcaattggg atgttaatat
     1741 tcgctaattt ttcatagtag ttattaaacg gatgtctaat gactaataaa tcacagccaa
     1801 tactttctaa agttttacat gtgtcatata aagattcacc ttttgaaaca gatgatgttg
     1861 atgtttcaaa gctaatcgtt tttaacccta gcttaagttc tgccatttca aaactacatt
     1921 ttgttcgagt agaattttca aagaataaat ttgcgacata tttcccttca aagtttggta
     1981 gttgacgttc accagattta aattgacttg ccttttggat aagtttgtat atttgatctg
     2041 tagataaatg ttccattgat aataaatgat tcataaaagc gccctcctta atttgtaaat
     2101 cttaattgtt ttgttttttc tctttaggta agattaagtt caaaataatt cctgaaagtg
     2161 cagctaatgc catcccctca atttgaaggt tgataccaat ttcttttaag ttaaatacta
     2221 aattaccgat acctacaact aaaattacag aagctataac taaatttcga ttgttcgcaa
     2281 aatctacttt gctttcaact aacattctta agccacttgc tgcaataata ccgaaaagta
     2341 atatagatac tcctcccata actggtgtag gtatagaaga aattaatgca gtgaacttac
     2401 caatgaatgc taaaacaatt gctataactg ctgcaccacc aatgacgtaa atactgtata
     2461 ttctggtaat cgctaataca ccgatatttt caccgtatgt tgtacttggt ggtccaccaa
     2521 taatactggc aaacattgta gaaacaccat caccaatgat tgatttatca agtcctggct
     2581 tttcaaagaa gtttctacct acgattttat tcaataccat ttggtgccca atatgttcac
     2641 ttactgttac gaacacaatc ggaatcatta caagtacaag tcctaagtga aatgaaggta
     2701 cataatcttt aaatggcaga taaatatgag ggaaatctat ccattttgct gacataattg
     2761 catcaaattt cacaatcccc ataaaaatcg ctaccacata acctacgata atacctacaa
     2821 gaactggaat taaagatagg aatcctttaa aaaatccttg aacgactatt gttacaagca
     2881 aggttatcat tgcaacaatt aagaaactga tattgtaacc tttcatatct ccaggatttt
     2941 catacattgc catattgact gcagtaggcg ctaagcttaa accaattacc atgatgactg
     3001 gtccaacaac aactggtggt aataatttca ttaaccatgc tgtcccactt aatttgatta
     3061 gaatcccgat gatgacgtac ataacaccac tcatgaataa tgctacaagc atgtctccta
     3121 agctatgcgt acttaatccc gtgataattg gcgtgataaa tgcaaagcta gatcccaagt
     3181 atgctggtat ttgcgccttc gttattaaga tataaagtaa tgtaccgatt cccgaagcta
     3241 gtaacgctgc tgatattggt agtcctgtta agaatggtac tagtactgtt gcgccaaaca
     3301 tcgcaaataa atgttgtaag cttaaaaatg cccattgcgc tggttgtggt ttttcattta
     3361 catctagtac gggttttact gttcgttcaa acatttcatc attttgcata atattcattt
     3421 cctccgataa taaaaaaatc tctttacatc agtatatgta aagagacaaa aagtgtgaca
     3481 agttgctaca agtcattttc gtccatagaa attgacttat agttgtcgaa catgagggta
     3541 ttattagata aacaagcata tgaaaactta tttatcattc aactccccca cctttttcag
     3601 tctctcgtac tgaattaaaa ggggtattat ttaattataa ctgcatttct ttgatccatt
     3661 tcttctaaat agacacttac cgtttcctct ttagaagtag gtatattttt accaacaaaa
     3721 tctgctcgaa ttggtaactc acgatgtcct cgatcaacca aagcagctaa accaatttta
     3781 ataggtctag catttagcaa aatagcatca agtgaagcac gaaccgttcg accagtatac
     3841 agcacatcgt caataatgat gactacttta tctgtaatat ctgtgtcgat gtctattgcg
     3901 tcttttgtcg taagtgatga catgtgctct atatcatctc taaagtatgt aatatcaatt
     3961 gttccagtag gtatacgttg ttgctcaatt tgatgaattt tatcttgtat acgattcgct
     4021 aaatattcac ctcttgtttt gatacctaaa agaattaaat tatcagtacc tttgttatac
     4081 tccaaaattt cgtgagcgat tctcgtcact gtacgttgta ttgcggcatc atccattatg
     4141 atacgttcag acatattctc accctttcaa aatttagaaa caaaaaacct catgtcttta
     4201 atatc
//
LOCUS       DANRDI010000191         4142 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.193, whole genome shotgun sequence.
ACCESSION   DANRDI010000191 DANRDI010000000
VERSION     DANRDI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.193"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..245
                     /locus_tag="PZ377_002529"
     CDS             <1..245
                     /locus_tag="PZ377_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359384.1"
                     /translation="SVIKTLVPDDDNRFVASVTQQPIQLLFNDNNQLYGFVYPIVDKK
                     ELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            254..1090
                     /locus_tag="PZ377_002530"
     CDS             254..1090
                     /locus_tag="PZ377_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359385.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(1351..2328)
                     /locus_tag="PZ377_002531"
     CDS             complement(1351..2328)
                     /locus_tag="PZ377_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDM6359386.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            2480..3517
                     /gene="mutY"
                     /locus_tag="PZ377_002532"
     CDS             2480..3517
                     /gene="mutY"
                     /locus_tag="PZ377_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDM6359387.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            3819..>4142
                     /locus_tag="PZ377_002533"
     CDS             3819..>4142
                     /locus_tag="PZ377_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDM6359388.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYD"
BASE COUNT         1441 a          576 c          715 g         1410 t
ORIGIN      
        1 gttcagttat aaaaacttta gttcctgatg atgataaccg tttcgttgca tccgttacac
       61 aacaaccgat acaattactt ttcaatgata ataatcagtt atacggtttt gtttatccaa
      121 ttgtagataa aaaagaatta aaagataagt ttaatattaa caataacatt tggattacta
      181 aagttgggaa tggatattgt attgccaatt tgaaagaaga caaatggatt tatattgaat
      241 tgtaggtgta aagatgctag ataatattat tttatatttt aaaaacttac cgcatactaa
      301 aaggtatgta acagaacgtt taaaacaaag ttggaaaagt tttctaatcg tattagctgc
      361 atgtttaata cttattattg caagtgaaac gctattttcc tttagtcatt tgaccgatgt
      421 taaagaggtg agatggttat ttagaatcat tgtattgatt gtttttgcgg tagtgatgtt
      481 cacaatttat atctcttatc atcattatat gaatgacttt ttagttacta agttatttaa
      541 tatttccgca gcgacgccag tagttattat gtctatttta agttttatta tgctagtcat
      601 tttgacgatg attagtgcat tggttaaacc agtaaacttc gaaacatcat atatagcgct
      661 attctatttt attgtgatgg caactatttt tgtaggatta atatccgtta cttttggtct
      721 tattcgactg ttaactgaaa aaataaatat catattttat ggggtttgtg tattgtgttt
      781 cttattgtta ccaattattt tcataccaaa tccaaatcat gtatttatca atcacatcct
      841 aatgctaaat ccaatgtatt acattgttaa tggtatagca caatctatca tatttggtat
      901 atcaagtatg gaaaacattc catatcattt ttactttatt ttattcttat gtttaatagc
      961 tgcagtaaat ttcgtattag caaggtatac gacacacgca atttataata aaacatctaa
     1021 agtgacacaa actgataatc aacaggatgt ttcaaatgat agtacagacg aggcggatac
     1081 ttcttcatga tgtttagctg acctgttgtg taaattaatc tgtgagaatc agaaacaatt
     1141 agaatatcat ctagatattg ttttcctata ctgattttga tatttcgagt cattaaaggt
     1201 agtttgttag caacaatata aattttagat tttgtaaaag cattcatagt tttacattaa
     1261 aaaaccggac agagtaaaaa tcgttttgat tttcattctg cccggttgtt cattttttat
     1321 agattaactt tagtgaataa taacttgaaa ttatttagca tacagtttac gttgtaactt
     1381 atcttctgta aatacccagc caatataaga gtggtctatg acattatcgt ttgttacatg
     1441 tgcaattgcc acaaatgaat aatgatcatt ttttaaataa cgcaaatcaa tcaaacgaat
     1501 ttcagtagaa ccatctgcta acgttgttgt ttgccaacga tagattgatg aaaagtttaa
     1561 aaaagtacgt atatctttat tacctttgac tttccataaa atggagtctg ttgataatgt
     1621 ttgacgttcc actttatcac taaacaccac attcctacca tatgcttttc caacatagtc
     1681 atgtgcatca gtttgtatcg cgacacgcca ttccataaat tttattgttg gcgcaacaaa
     1741 tactttaaca gggtgatgct cttgttgaat tgcttttaaa gcttgttgct taattacggc
     1801 tctcatttta aaacgaatca tgtaataaat gattagtaaa gcgattatag gaaagaagac
     1861 tgcaaatgga tgcaagccta taacccataa tacaatacca atacaaagaa ctgtgaaaat
     1921 aatagggtca aatgtgttaa tcacacttaa ttgaatccat ttgtttgtga taggtctaag
     1981 cgcttgtgta ccataagaat tgaatatatc tacaaagaca tgtaaaaata ctgcgagctg
     2041 agcccacatc catacatgaa atgggttggt tccactaaag aacgtgaata ttaaaaatgt
     2101 aattaaaatt ggccatagta ttgtgaaagg gatggaatgc gtgatacctc tatgatgcga
     2161 aatatatgtt gcattgtcct ttaatttaag aacagtatcc ccatcaggaa ttaatgaacc
     2221 aacgataagg gttgtagctg ttgcaccaaa cgtagaagcc attgctggat cttgagttgc
     2281 aagtgctgta aggcccaccc caattgcgat atgtgttgca gtgtccatat cagttcactc
     2341 atttcataat attatatata ctattataat ccaatttcgc aaataactaa attaacttac
     2401 atactaacca tgattttaat gtacaataaa aacattatga gtaaagacaa ttaattttgg
     2461 tgaggaaagg gttttataaa tgtatcagca gtctagtttt aaagaaaatt tgatacactg
     2521 gtttgatgaa aatcaaagag aaatgccatg gcgacaaacg accaatccgt actatatttg
     2581 gttaagtgag gtcatgttac aacagacaca agttaaaaca gttattgact attatcatcg
     2641 ttttgttgaa cgatttccaa ctgtggaagt tttgagtcaa gcttcggaag atgaagtgtt
     2701 aaagtattgg gaaggcttag gatattatag tagagctcga aattttcata cggcgattaa
     2761 agaagttcat gataaatatg aaggcttagt tcccaaagat cctgatcaat ttaaagcgtt
     2821 aaaaggtgtt ggcccataca cacaagctgc agtaatgagc attgcatata atgtaccact
     2881 agcaactgta gacggaaatg tttttcgagt atggtctaga ttgaatgatg actatcgaga
     2941 tattaaatta caatcgacga ggaaatcgta cgaacaagaa ttattaccgt atgttactac
     3001 agaagctgga acgtttaatc aagctatgat ggaattaggg gcgttaattt gtacaccaaa
     3061 aaatccatta tgcttatttt gtccagttca agaaaactgt gaggcatttg ataaaggtac
     3121 atttgaaaag ttacctgtta aatctaaaaa tgtaagtaaa aaagtaattg agcaatccgt
     3181 ctttttaatt agaaataatc aaggtcaata tctgttgcaa aagcgtagcg aaaaattatt
     3241 gcatggtatg tggcaatttc ctatgtttga aagtgagcat gctagacgta aaatgactga
     3301 aaaaatcggt catgacattc aaccagtaga aacacctatt tttgagttga agcatcaatt
     3361 tactcattta acatggaaga ttaaagtata cgccgtttca ggagcaatta acattgaaac
     3421 attaccagat gacatgattt ggtttgattt aagtgatcga gatcaatata cattccctgt
     3481 acctatgtca aagatttatc aatttataaa tgggtaacat tcgatgattg gtaatattat
     3541 cggaaaacat agtgacatta attttaaaaa gtataaatga tatctttaaa tctcatagaa
     3601 taacaatgtc aaaaagttaa agtgttgata tataaaagtg atgattttat atactttaaa
     3661 tgggacatgt aaatctctgt tgtagtactt agcaatatta ccgagtttaa ttattaagta
     3721 gtaaaatctt atttcgtaaa aatatgtatt tatataatac tatttctgaa atatgctata
     3781 atttataatg gttaaattaa cagaaggtgg tgtggagtat ggtcagagaa tccataccta
     3841 aagaagggga aaacattaaa attcagagtt ataaacatga tggtaagatt catcgcgttt
     3901 ggtctgaaac aacaatatta aagggtactg atcatgttgt cattggtgga aatgatcata
     3961 cgttagtgac tgaaagtgac ggaagaactt ggataacacg tgaaccggca attgtttact
     4021 ttcactcaga atattggttt aatgttatct gcatgtttag agaagatgga atttattact
     4081 attgcaattt gtcatcacca tttgtttgtg atgaagaggc attaaaatat atagactatg
     4141 at
//
LOCUS       DANRDI010000192         4084 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.194, whole genome shotgun sequence.
ACCESSION   DANRDI010000192 DANRDI010000000
VERSION     DANRDI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.194"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(239..985)
                     /locus_tag="PZ377_002534"
     CDS             complement(239..985)
                     /locus_tag="PZ377_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDM6359389.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(990..1463)
                     /gene="ytkD"
                     /locus_tag="PZ377_002535"
     CDS             complement(990..1463)
                     /gene="ytkD"
                     /locus_tag="PZ377_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDM6359390.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1529..1786
                     /gene="yidD"
                     /locus_tag="PZ377_002536"
     CDS             1529..1786
                     /gene="yidD"
                     /locus_tag="PZ377_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDM6359391.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1783..2784)
                     /gene="menC"
                     /locus_tag="PZ377_002537"
     CDS             complement(1783..2784)
                     /gene="menC"
                     /locus_tag="PZ377_002537"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDM6359392.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2789..>4084)
                     /gene="menE"
                     /locus_tag="PZ377_002538"
     CDS             complement(2789..>4084)
                     /gene="menE"
                     /locus_tag="PZ377_002538"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDM6359393.1"
                     /translation="LIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLPLEL
                     RGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNLDDI
                     ASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLSVLL
                     RAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQKILL
                     GGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANVDVK
                     IKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIYDRR
                     KDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISKAQL
                     IAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
BASE COUNT         1209 a          869 c          639 g         1367 t
ORIGIN      
        1 taaagagcca accgttgtta tagtctaaca atggttggct cctcttattt tatgtgctaa
       61 aaatttatag gcaattttat tacaacaatg tacatttaag gtgaccttca tgccaaaatc
      121 gcatcactca tttaatggaa gcagcacgtc ttcatataaa gtaccgatcc ctaattcaac
      181 gcatgtagta ccacatcttc aaagcttgat agttcccatg cgcacaccac gtttcatact
      241 agctatgcga ctcaacttgg ttcataaact ctttaatata agtcaatgtt tcaaccatcg
      301 ctggtggtct tggcacatgt ccttctgcca tttgataaaa tgtttcatgc gtggcacctt
      361 ttaactctag ttggtccgct aaataatacg catgatgaat accaacttgc tggtctttcc
      421 ctccatgtac aattaatatt ggcggactgt tttcattaat gtttggaatc gcttggcgtg
      481 cctcatatgc cgctcgatct tttttcggat gaccaatcat tcttcgtagc atgcctctta
      541 aatcgacacg ttcttcatac attaaatcaa tatctgagac accaccccag attgtataac
      601 ttgttactgg taagtcttga aatgtcaaca atccttgtaa accacctcgc gaaaaaccaa
      661 ccatgtggat aaatgcatgt ggatatttat catgtagcaa ccttaataat tgcgtcacat
      721 catttaaatc gccacggtaa aattcgtctt tgccttcact cccattgtta cctcggtagt
      781 atggcccaat cactaaagtt tgactatctg aaaattgcat taatctacct gcgcgcacac
      841 gtcctacttg acctttgcca cctcgcaaat aaactacaat gcgatttact tcatgatgtg
      901 gtgtcatcat taaagctttt acttgtaagt catctgacaa atatgtaatt tcttcgaatt
      961 gatgcgtaaa atattcaatt ggcattcgtt tacgtttgat aaaacccaag tgattgcacc
     1021 ctctctacgc attttaaaat ggtactatct tgcagtaaga aactccgttg tgcgagttca
     1081 atatcattga tacagttaaa caacactggc cctgctgttt ctaaataatc gttcttgctt
     1141 accaatgatt caacttcgat aaaatataca tcttttacaa aatcagtttg atcatgtgtt
     1201 tcaatggtat attgtgctat gtaataaata tttttaactt tggcgcctgt ttcttcatat
     1261 aattcacgtg taactgcttc agcactactt tccccgcgtt cccttttacc accaggaaat
     1321 tcaatccccc gtaaattatg tttggtaaaa agcaattgat ttttaaacgt tggaatagct
     1381 agcacatgat tgccatctgc tatctcatta tcctttttaa atgtcaaatt aacttgacga
     1441 ttatctttat ccctaaactt cacgcgcatc acatccctac attgtatgtt aatataatag
     1501 ttaattacta tcgttggagg cattaattat gaaaaagata ttcttggcga tgattcattt
     1561 ttatcaacgt ttcatttcgc cactcactcc accaacttgt cgtttttatc caacatgttc
     1621 agagtacact agagaagcga ttcaatacca cggtgctttc aaaggccttt atttaggtat
     1681 ccgtcgtatt ttaaaatgtc atccgcttca taaaggcggc tttgaccctg ttccgttaaa
     1741 aaaagacaag tcagcaagca agcattcaca taaacataac cattaatatg gttgtaattg
     1801 agttatatcc actaaagggg ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg
     1861 cgccaccaca tcttgttcaa aataataacc tgctggtgta acatctcctg gataatcacc
     1921 tttacgagca agcatcgctg taaaatagcg gcttaaacca tattcgtaca tgccgccaat
     1981 aaccactttt gcaccatgac ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc
     2041 acctagacga aatggtttta atacaacaac tttcacattg tataattcta tcaaattaat
     2101 tatgtccaac aacgatgttg ccttttcatc aagggctatt ggaggtattg ttccatccgc
     2161 tacttcatca agcatggaga tatctttaaa tggctcttcg atataaagaa cctgttcacg
     2221 cgctaataac tgtaactgtg tgaaatcttg acgatccaag gactcatttg catctataac
     2281 caattgaaag tgaaagtcta attcccgtaa cactctaatt tgatgcatga tttgaggcgt
     2341 ccattttaat ttaattctgg tcggctttgt tgcttttaat gactctagtt gtttatttga
     2401 taagccgctc gctgtcgctc catatgctac tgaaaatgaa ggcagtacat gaaacatttg
     2461 atacaatgcc atgacaatag ttgcccttgc agcaggcgta ttttccaatg aatctactaa
     2521 ttttagtgct gcttcatacg tttcaaatga tttatttcta ttatcttcga accattgctc
     2581 aattacatgt ttcactgagg caattgtttc atgatcatac caatctgttt gaaaagcgtt
     2641 acattccccg aaatatgcat ttcctttgtc atcaatcaat tcgataaaca aacaatcacg
     2701 atgcgttaaa gtgactttcg gtgttacaat ttgtgactta aatggctcac tatatttata
     2761 aaaatgcaaa gctgtcaact tcatcaaatc atcctctata caacttattt ctttgtaatt
     2821 tacctgttga tgtataaggt aaagtatcaa ccttttcaaa gtgtttcggt actttatatt
     2881 tcgctaaatg ttgtgataaa tatgcaatca attgtgcctt tgaaatgtca ctttcactga
     2941 caaaatataa tttaggcact tggccccaag tatcatcagg atgccctaca catactgcgt
     3001 cactgatacc tggaaattgc ttcgctaccg tttcaatttg atatggataa atattttcac
     3061 cgccactaat aattaaatct ttacgtcggt cataaatcat gacataacct tcatgatcta
     3121 tttcagcaat gtcacccgta ttaaaataac cattttcaaa cgtacccgtt aaatctgttg
     3181 gatacaaata tacattcatc acattggcgc ctttaatcat taattctcca tgaccttctt
     3241 tattaggatt tttaattttt acgtcaacat tggcacttgg catccctaca gtgtcaggac
     3301 gtgcatgcaa catttccggt gttgctgtta aaaattgcga acatgtctca gtcataccaa
     3361 atgaattata aattggcagg ttatattgta atgccgtctc tatcatagtg gcagataatt
     3421 tagcaccgcc gagtaatatt ttttgtaaat tataaggttc atgtaaacct tgttgcataa
     3481 gccaatttaa agtttgtggc acaagcgaaa tgtgcgtgat tcgttcattt ttaatcatcg
     3541 ttaaaatttg ttcggcattg aatttatcaa caatgcgcac agtaaaacct tcaataacag
     3601 ctcttaaaag tacactgaga cccgaaatat gataaatcgg caagacagat agccaattag
     3661 tgtcacgatc aaatcccaag ctctctttac atccgattgc actggcataa tgattacgaa
     3721 acgtttgtgg caccgctttt tgagggcccg ttgtccctga tgtaaacata atcgatgcaa
     3781 tgtcatctaa attaaatgat gtatttaata tgttggacgg cgactctttc ggcaccacag
     3841 tttcattcga tgtttcatat tggataccca ttgtgttgtc caacaaactg ttcgttgtaa
     3901 tatcccttcc agcgaattca atatcatcca gcgatacaat ttgaaaccct cgtaattcca
     3961 gtggcaaggt acaaaaaatc aattgtacat cgattgactt catctgattc gtcatctcat
     4021 taggtgtcaa ccttgtatta atcatcgcaa tttcaatatt tgccaaccaa catgcatgta
     4081 ttaa
//
LOCUS       DANRDI010000193         4040 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.195, whole genome shotgun sequence.
ACCESSION   DANRDI010000193 DANRDI010000000
VERSION     DANRDI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.195"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..482
                     /locus_tag="PZ377_002539"
     CDS             <1..482
                     /locus_tag="PZ377_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359394.1"
                     /translation="IQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDFE
                     DNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRLS
                     NEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI"
     gene            484..651
                     /locus_tag="PZ377_002540"
     CDS             484..651
                     /locus_tag="PZ377_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDM6359395.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(791..2638)
                     /gene="typA"
                     /locus_tag="PZ377_002541"
     CDS             complement(791..2638)
                     /gene="typA"
                     /locus_tag="PZ377_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDM6359396.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            2740..2931
                     /locus_tag="PZ377_002542"
     CDS             2740..2931
                     /locus_tag="PZ377_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDM6359397.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(3085..3912)
                     /locus_tag="PZ377_002543"
     CDS             complement(3085..3912)
                     /locus_tag="PZ377_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDM6359398.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
BASE COUNT         1291 a          747 c          599 g         1403 t
ORIGIN      
        1 tgatacaaat taaaggagcc gtaaagtttc ctataacatt agatagtacg acttggattt
       61 tcgatgatag aaaagttagc atcgaagatt tagaaaaagg tgtatttgat ggttcaaaac
      121 caatcgactt tgaagataat catgaatgga atcgtgctat cctagaagga caaactaatc
      181 cgcctacttt aaattcagaa ataaaatata aaaaacgtgc catgttagaa ggttcatttg
      241 tcattaatat gacaccattt ttcaaacatt ccgaaccaca tgactctgca actactattc
      301 gattatcaaa tgagcatgat catttagata taccgataga tctattaccc tatttatttt
      361 tccaatttgc aaaagacggt aaacgacttt acagcgataa tggtgtagat agttttattt
      421 acaatcaaga agatggttat tcatatcgaa tcaaccatgt aactcatatt gaggtgattt
      481 aatttgagac aagtgcaatg cattatctgt gatgcaaaag tatttataga tgagcgcaca
      541 actgagtcta aacgacttaa aaacaatcct attcgaacat ttatgtgtga tgattgtaaa
      601 agtcgattag acacacctaa acaacgtgcg caacactatc cgctcgatta atatctgaat
      661 aaacaagtta gaaatttgaa aatgtgaaag taacaggaaa taatagcatt taaacacttt
      721 ttcaaatatt atatgccaaa agagaagcta agcaactttc atcgctcagc ttctctttta
      781 ttatgttaaa ttattcgttt tcttgcatca tttgtttgat acgctttgct tctttttcac
      841 gaacattttt gtttaaaatt ttctttctta aacgtatact ttctggtgta acctcaacaa
      901 gttcatcatc attaatgaat tgtaacgctt cttccaatgt tagaatacgc ggtctattca
      961 ttgtttgtgt ttggtctttc gttgcagaac gtacgttagt ttgatgtttt gttttagtga
     1021 tgttaacagt taaatcattt tcacgattat gttcaccaac aatcatacct tcataaactt
     1081 cagtaccagg ttccatgaag tttacacctc tatcttcaag tcccaaaatg gcataagtac
     1141 ttgcagaacc ttgatccatt gaaattaatg caccattacg acgaccgcca atttgtgctt
     1201 taatacgtgg tctaaattct tcaaatgtat ggttaataat accgtaacct cttgtcattg
     1261 acataaattc agtcgtataa ccaatcatac cacgagccgg tacattaaag attaaacgtg
     1321 taagtccatt atcagttgta gtcatatcaa ccatttcacc tttacgtgca cctaatgatt
     1381 caataacagc acctgcattt tcttgtggca cttcacattg cacacgttca aatggttcac
     1441 acattacacc atctatttct ttaataatta cttgtggttt tgaaacttgt aattcataac
     1501 cttcacgacg catattttca ataaggattg ataaatgcaa ttcaccgcga ccagcaacta
     1561 cccatgtatc tggagaatct gtgttagaaa ctttcaaaga tacatctgtt tctaattgtt
     1621 gatttaaacg ttcttgaatt tgacgtgctg ttacaaagtc accttcacgg ccagcaaatg
     1681 gagaattgtt aactttaaat gtcatttcaa gagtaggctc atcaatacgt agaactggca
     1741 atgcttcttg atggtcatgt ggtgttacag tttcaccaac attaatgtct tccatacctg
     1801 aaacagcaat taaatctcca gcttgtgctt cttcaatttc taaacgtttt aatccaaagt
     1861 aaccaaagat tttagttaca cggaagtttt tcactgtacc gtctaattta attagtgata
     1921 cattatctcc gacacgcatt ttacctctga atacacgacc aataccaata cgtccaacat
     1981 aatcattgta gtccaacaat gctacttgga attgtaatgg ctcatcactg ttatcaattg
     2041 gagctggtac ataatcaata attgtttcat ataatgattg taaattatca tcttgctttt
     2101 caggatctaa gctagctgta ccatttactg ctgaagcata aacaacaggg aattctaatt
     2161 gttcatcgtt tgcttctaat tcaataaata aatctaaaac ttcatctaca acaccctctg
     2221 gacgtgctga tggtttatca attttattaa caacaacaac aggtttcagg ttttgttcta
     2281 gcgctttttt aagtacaaaa cgtgtttgag gcattgtacc ttcatacgca tctactacta
     2341 agacaacccc atcaaccatt ttcataatac gttctacttc tccaccaaag tctgcatgtc
     2401 ctggtgtatc caaaatatta atacgtgtac ctttataatc aacagccgta tttttggcta
     2461 gaatcgtaat tccacgctct ctttcgatat cgttagagtc cattgcacgt tcatcgacat
     2521 gttcattttc tctgaatata ccagattgtt ttaacaactc atctactaaa gttgttttac
     2581 catggtcaac gtgagcaata attgctatat tgcggacatc ttctctttta ttagtcatat
     2641 aaatagtttc cttctttctt aagtaaaatc ccacttttaa ttacaacttt tttattatat
     2701 catataattg agaaaagata agcaaaaggt ggggatagga tgaaacaaaa aaaatctaaa
     2761 aatatctttt gggtattttc tatattagct gttgttttct tagtattatt tagttttgct
     2821 gttggtgcat caaatgtacc aatgatgatt ttaacattta tattactcgt tgcaaccttt
     2881 ggaattggat ttactacaaa gaaaaaatat cgagaaaacg attggctata agttatatcg
     2941 acaataacac gcgttgattt aagaccacac atgttcatga tgaacattta actggtttta
     3001 tattggcgcg tgtttttatt atttaaggta taaaagcgta atatgttacc taattgttag
     3061 gactacatat tacgcttttg gtaattattt tgattttctt ttaaatcgtt gttcatgtaa
     3121 ttgtattaac gcatcatggt ggggctctaa ataatcattg ctaatttctt gatggagacc
     3181 acgatttcca actaagattg aatttggacc actaattgtt aatggttctc ctagtaaatt
     3241 ggaagcttgt ccatttactt catataaaat aaccaatccg ccagcaaaat cccatggttg
     3301 aagtcttggc gtcatatatg cttctaaatt acctgtagca actgaaacga tttcaagcgc
     3361 tgcactacca tatgcccttg cacttctaga atcattaaca atttctttaa agatttctcc
     3421 taaaattggt ttagttaacc agttcggatt gatcccaata atgctttgtc ttagattaga
     3481 atcattcaat ggtttcaagg gttggctacc acgatatgca ccttccccta ctttagcatg
     3541 atataagaca tcagccataa catcatatac aaaacctgca taaggtttac catcgatata
     3601 aataccaatt gaaattgcga aattttcttg ttgatgaaca aaattcaatg taccgtctat
     3661 tgggtcaaca atccataccg tacctttgga agtatcgatg tcatgaccat gcccttcttc
     3721 acctaatact tgatgattgg gatatgtttc taaaattgta tcaaaaatga aatcttctgt
     3781 tgctttatct acatttgtaa caaggtcatt cggatttgac tttgtttcaa ttgttaaatt
     3841 ttgctccatc aattgtttaa ttctaattcc tgcttcttga ataagtcctt gggcaaatcc
     3901 ataaagtgcc ataacatcat ctcctaacat ctttattata catcaacatt ttataaaaat
     3961 aacttcttat gataaaaatg aaaatactaa ttttaatgag agcgtcttaa gccgcaattg
     4021 ataaaacata tgctacaata
//
LOCUS       DANRDI010000194         4008 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.196, whole genome shotgun sequence.
ACCESSION   DANRDI010000194 DANRDI010000000
VERSION     DANRDI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.196"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..655)
                     /locus_tag="PZ377_002544"
     CDS             complement(<1..655)
                     /locus_tag="PZ377_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDM6359399.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     "
     gene            complement(683..1087)
                     /gene="rbsD"
                     /locus_tag="PZ377_002545"
     CDS             complement(683..1087)
                     /gene="rbsD"
                     /locus_tag="PZ377_002545"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDM6359400.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            complement(1102..1983)
                     /gene="rbsU"
                     /locus_tag="PZ377_002546"
     CDS             complement(1102..1983)
                     /gene="rbsU"
                     /locus_tag="PZ377_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDM6359401.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            complement(2215..3213)
                     /locus_tag="PZ377_002547"
     CDS             complement(2215..3213)
                     /locus_tag="PZ377_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDM6359402.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            3364..3543
                     /locus_tag="PZ377_002548"
     CDS             3364..3543
                     /locus_tag="PZ377_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDM6359403.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVIKNTKVIILKNKIFVFEQ
                     SIKKHNEMMYIELCD"
     gene            3593..3985
                     /locus_tag="PZ377_002549"
     CDS             3593..3985
                     /locus_tag="PZ377_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359404.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT         1301 a          637 c          727 g         1343 t
ORIGIN      
        1 cagtcttaat gcctatagat aaaaagtaat tggcattgtc tttcatagat tgttcattag
       61 ttactttaat cccagataac aattcggctt ctgtttcgtt tggcacaata atatcgatta
      121 atgataataa ttcattaggt aatgctttcg ctggtgcagg atttaatact gtcgtcacac
      181 catgtgcctt ggcaatttca aatgcagata taatagccgg gatgggtact tctaattgtg
      241 caacgacaaa gtctgcattg attatagcgt cttttgcgtt aataacatct tcaggtgtca
      301 tcgtcatatt cgcaccacca taaacataga tggtgttttg tccttctgca ttcacagtga
      361 taaaggcttg gcccgttttt gcttcagctg ttttgataat atatgatgta tcaatatgag
      421 ctactttaaa atcttctaag atgaaatcag caacgccatc agtgccaatt ttagtaataa
      481 atgttgtgtc tgcttgcatg cgtgcagtgg caatagcctg gttggcacct ttacctccgc
      541 cgaatgcttt ttgtgcttct tcaacatgta atgtttcgcc tggttgtgca tatctttcaa
      601 ctgttaaaaa ttgatcgaca ttcgttgaac ctaaaataac aactttgttg gtcatgtgta
      661 cgctcctttc aagttataac ttttaaaaag taacattcga ttctaatgca atattagagt
      721 agggcgttgt ttcaccagta cgaatattac ctttatttaa tgggtgagct aagttacttt
      781 tcatttcttc gtgaggaatg aaaatgattt cgatttccga tgaaatcaat tgtttaattt
      841 gttgcaattg tgtagggtta tgttctttta tttcttctgc taagtatatt ttttggattt
      901 ccatttcttc taacactgta gctaagacat caataaagcg tggtaagttt ttagttacag
      961 ctaggtcgat acgacgatga tcatttggaa ttggcatgcc agcgtcatta atcgttaata
     1021 aatcaaaatg accaattgtc gcgattgctt ttgaaatatg ttcatttaaa atagctgatt
     1081 ttttcatgac atctacactc cttattttat aaatactgta acagaagcgg ctactaaaat
     1141 gagtactaag ccgatgattg taataaccat ttcttttgac gttttatgtt gttttaagaa
     1201 ataaatacca gttaatgtag caagcacaac ggatgtttga gaaagaataa atccagttgc
     1261 taaaccattc atattaggtt gtgctgaaat aagatatgtt aaagcaccaa atgcaaagaa
     1321 gaaacctgaa ataatttgta accacgtaat tttattacgg aatggattct ctgctttcat
     1381 attcataaag ccataaatga ctgcaacaat taccataccc attgcttgag gtaaaaaggc
     1441 agttaggcca tcaatagaag ttgcttgcgg tgcagctgaa tataaccagt atccaaattc
     1501 accaattaac agaagtacca ctgcacgacg taaatttttg gcgttacttg cttctttgcg
     1561 ttcactccaa actgtcatac gcgctccaat tagaataacg actaaagctg taaatccaat
     1621 gattttatga ccaatgcctg gccaatttcc taatgcaaag acaccccata aagatgcgcc
     1681 taataattgg aatgctgttg tgactggcat ggcacgagat gagccgacta attcgaacgc
     1741 tttaaatgta atgatttgtc cgaatcccca tcctgcacct gataataagg cgaatagcaa
     1801 attggttcca gtaggtaagc cacttgatgt gactacggct aatataatag cgaagattaa
     1861 cgtacctaca gtagcaccga taatttgatg tacaggttta ccaccaaact ttgaagcgac
     1921 tgttgggaag aagccccagc caattaaggg gcctaacccg ataagtaatg caacaatgct
     1981 catgttatac acctcatgat ttgtttttag taaaacgttt taccagtgcc atcgtaccat
     2041 ttttatttgt aaaatgcatg cgttttcata aaaactgaaa acgtttaaat catatttaaa
     2101 aattcataca ttattatttt aatttcttag taaaataatt atgattaaaa accctcagca
     2161 ttaaagcaac gctaagggcc taacaatgat gagtatattt cggaagatac gtagttagtt
     2221 tgaaagatga tagccagttg ttgcacgaat ttttaaagtc gttggtaatt caatcatatc
     2281 aatggattta tctaagtgct gtaatcgttg aagtaataag gttaaagatg ttttgccaat
     2341 atcagttata ggttgtgcca cagtagttaa aggtggcgag acgtacgctg cataatcaat
     2401 gtcgtcataa cctattaatg agatatcttt cgggatactg atgccatgtt caattagtcc
     2461 tcgtaaaatg ccaatagcga gttcatcgtt aatagcgaag attgcagtgg cagattgaac
     2521 catgatgtca tcaacaatgg ttagcccacc gcgcttagat aattcagtat ggacgatttg
     2581 tggttctggc aattgattcg cgcgcaaagt atcgacaaat ccagcgacac gagtcgacat
     2641 attcgccatc atgtcatatg gtgcaacaat tatcatatgg ttgtgaccga gttctattaa
     2701 atgttgtgct gcaagttgtc caccttgata ttcatttgtc cgaacaaaat ctgtatagcc
     2761 ttgatggtca ttttgatcca gtacgacata aggtacatga tgtttcttta gatagttatt
     2821 tagggcgtcc ggggatgata tgtattgtgc gataattaat ccgtcaatac ctcgatcaat
     2881 taaatgttta atattgtcat acaaatcagt tgctgtagat gttaaaaagc ataaatcaac
     2941 atcagatggt ttatggtcat gaatactttg catcagtgct gagaaaaacg gatttgttaa
     3001 gctaggcaaa atgacgccaa tagtttgaat tttactgccg cgcaattgtt ttgcatgttt
     3061 attaggggca tagcctaaac gttctgaaac agcatgtacg ttttttatcg ttgttgcgga
     3121 aaaacgacta tcattatgat ttaaaatatg tgacacagtt gtaactgata caccagcttc
     3181 tctagcaaca tctttaattg acactttttt catattatat tccttctctt tattgtgtaa
     3241 ttaatcatac agttatatac ccgattatta tattcatcat atcatgataa acatttgatg
     3301 acatatgaag acgtgaatga cttttattgt ctacaaacag acgtctgaag tatacgatta
     3361 aggttgagac gagagaatat attattaagg ttaatacctt ttaatgatta tgataacaag
     3421 aattttaaaa tgtttattgt gattaaaaat acaaaagtca ttattttaaa gaataaaata
     3481 tttgtttttg aacaaagtat taaaaaacat aacgaaatga tgtatattga actatgtgat
     3541 tgaaaaaatt tttaattaag gcttgttaaa acttaagaga ggatgttttt aaatgcaatt
     3601 caaattaaaa gaagaagaga ttattagttt tttagaattg aaatatccag aaaaagagtt
     3661 cgaatatggt cgtttgttag ttggacaaca taaacgtgat gatttagatg tttattactt
     3721 tggtgatacg tttttaatgt gcacgattat ttcattcaag acatttgaaa ttaaagaaac
     3781 agtagaatta tcatatgatg ctgttaatcg tattgtgtta aaagatggat ggttatttag
     3841 aaaaatgaga atagaaacaa tgcaaaaagt gttaaaatac ggtacatcta aattaatgtt
     3901 aactgatttt caaaaagaga attataataa atatattcaa ggtcagaaac aacgcgtgat
     3961 atttgaaaat ggccattttg tctaattgat agtgaatata attagagt
//
LOCUS       DANRDI010000195         3978 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.197, whole genome shotgun sequence.
ACCESSION   DANRDI010000195 DANRDI010000000
VERSION     DANRDI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.197"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..296)
                     /locus_tag="PZ377_002550"
     CDS             complement(<1..296)
                     /locus_tag="PZ377_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDM6359405.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLD"
     regulatory      451..561
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            667..1860
                     /locus_tag="PZ377_002551"
     CDS             667..1860
                     /locus_tag="PZ377_002551"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDM6359406.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            1985..2893
                     /locus_tag="PZ377_002552"
     CDS             1985..2893
                     /locus_tag="PZ377_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDM6359407.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            3105..3938
                     /locus_tag="PZ377_002553"
     CDS             3105..3938
                     /locus_tag="PZ377_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDM6359408.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         1418 a          567 c          758 g         1235 t
ORIGIN      
        1 tagtctagta ctttatggta taacttcaag aaagtttctt catcgatagg ttgattaatt
       61 tctccccaat caatgttatc tctatatgaa ggttcagaga caaaaaattt gtctttaggc
      121 gaacgaccag tatattttcc agtacttgca ttaacagcac caagttctgt taatacccct
      181 tcgttattgt ctaagatttt attataaagt tgtgtcgtcg aaagttgaaa atgtgacgtt
      241 ggttttttca gtaatttgtc aattttagtt gtttcagtgt atgtgtctac tgacatccta
      301 aatccctcca aagcgataat ttaaatgtaa gcctttacat ttaattagta taacacatta
      361 acataattaa tctacaatat tttaccgtaa atttatttgt gaaaaattga cattaattca
      421 ttaattaggt actattattt ttaacggatt ctcttatcct gagtggtgga gggacatgga
      481 cccaatgaaa cccagcaacc tcttttttat aaaagaaagg tgccaaaccg tttgcagaca
      541 aataggtctg aacgataaga gcgaatggac gtattaggcc ttctctctat attaatagtt
      601 agaaggtctt ttttatttag ctcacagaga gagaattttc gtaatataaa tttaaaggag
      661 caaactatgt taaataacaa acgattattt acttcagagt ctgttacaga aggacaccca
      721 gataaaatcg ctgaccaagt gtcagatgca atattagatg ctattttaaa agacgatcca
      781 aatgcacgtg tagcttgtga aacaactgtt acaacaggta tggcattgat cgccggcgaa
      841 atttctacaa caacatatgt tgatattccg aaagttgtta gagaaacaat taaagaaatt
      901 ggatacacaa gagcaaaata tggttatgat tatgaaacaa tggcaatttt aactgcgatt
      961 gatgaacaat cacctgacat tgcacaaggc gtggataaag cattagagta tcgtgataaa
     1021 gatagcgaag aagaaattga agcaacaggt gcaggtgacc aaggtttaat gtttggttac
     1081 gcaacgaatg aaacagagac gtatatgcct ctagcaattt atttatcaca tcaattggca
     1141 aaacgtttat cagatgtgcg taaagatggt acgttaaatt atttacgacc agacggtaaa
     1201 gttcaggtca ctgttgaata tgatgaaaat gataacccag tacgtattga tacgattgtc
     1261 gtatcaacac aacatgctga ggatgtgaca cttgaacaaa ttcaagaaga cattaaagca
     1321 catgtcattt atccaactgt tccagaaaac ttgattaatg aacaaactaa attctacatc
     1381 aatcctacag gacgctttgt aatcggtgga cctcaaggtg atgcaggatt aacaggacgt
     1441 aaaatcattg ttgatacgta cggtggctat gcacgtcatg gtggcggatg cttcagtggt
     1501 aaggatccta caaaagtaga ccgttcagct gcatacgctg cacgttatgt ggctaaaaat
     1561 attgttgcag caggtttagc agatcaatgt gaagtacaat tggcatatgc gattggtgtt
     1621 gcagaaccag tgtcaattgc aattgataca tttggaacag gtaaagtttc tgaagggcaa
     1681 cttgtcgaag cagttagaaa acactttgac ctaagacctg caggtattat taaaatgctt
     1741 gatttgaaac aaccgattta caaacaaact gctgcttatg gtcattttgg acgtacagat
     1801 gtcttattcc catgggaaaa attagacaaa gtggaagaat taaaagacgc agtaaaataa
     1861 taagatgaat tgaatatagc taaataaaag attttcggta tataaatgat aatatgtatc
     1921 gaaaatcttt agcttttcta ttataattaa agttattcat atattaaaaa ggagcgttgt
     1981 tgtgatggat ttatcttcac cgatagtcat tggactcatt attgcagtag tcattgcatt
     2041 aatctttttt gtcctttttt tagttgcctt aggtagtaag aaaaaagtta agcgacaaac
     2101 agaagaaaag tatgaacaac aagaacaaaa cattaaaaag tctcatgaag aagctttaga
     2161 gaaagagcgt attcaaaata aaaagacaat tacaaaacaa caagaagatt ataatcacat
     2221 ggttagtaca aaagatcgtg agattgacgc attaaaattg ttctcgaaaa atcatagtga
     2281 atatgtaacg gacatgagat taattggtat tcgtgaaaga ttagttaaag aaaaacgaat
     2341 tcgaccagaa gatatgcaca ttatggcaaa tatcttttta ccaaaggatg gctttaataa
     2401 tattgaacga attagtcatt tagtattaac aagaactggt ctttatatta ttgattctca
     2461 attgttaaaa ggtcatgttt ataatggtat tagtggcggt caatttaaag atttaccacc
     2521 aatggaacaa gtatttgata cattggattt agataaatct agaccgcaaa ctattgtgat
     2581 ggatcaaaat gatgacaaac gttcattatc atttgtaaat tattcagatc aaattgaggc
     2641 gattaaacaa ttggctgaag acttacaaaa agaattaggt gccaaatata caccaacttc
     2701 aattttatac tttaatctta aaaatgaagg cgatgtaacg atttcaaatt ataatcaaaa
     2761 tagtgctgtc aaagtgcttg tcggtgctga acaattagac gagttcttta acaaatttgt
     2821 attccatggt cgtattcaat acaacgtgga agacttacaa caaatgatgg ataaaattga
     2881 atcattcaat taaatgttag gctgaagtga tgactatatg ttgttacttc ggctttttta
     2941 gtttgtcata aattaaacag aattactttt acaatttgat taatatttga gagatattac
     3001 tagaatttta gtggttgatt gctctaagtg ttttttctca tgtattcaac tcagatttgt
     3061 tatgataaca gggtataata catcaaaaag gtgaggcgat atttatggag gttaaaacat
     3121 tttataatgg aaacacgatg ccacaaattg gtttagggac atttcgtgtg gaaaatgatg
     3181 aaaattgcat ggaaagtgtt aagtatgcca ttgaacaagg atatcgtagt attgataccg
     3241 caaaagttta tgggaatgaa gaacaagtag gtgctggcat tcgtgccgga ttggagtcaa
     3301 ctggtattgc tagagaagac ttatttatta cttcaaaatt atattttgag gatttcggtc
     3361 gtgaaaatgt agcagctgct tacgaagcta gtttatctag attaggttta aaatacttag
     3421 atttgtatct agtacattgg ccaggtacga acgaagccgt aatggttgat acatggaaag
     3481 gtatggaaga tttatataaa aataataatg ttaaaaatat aggtgtcagc aattttgaac
     3541 ctgaacattt ggaagctttg ttggcacaag tgtcaattaa acctgtaatt aatcaagttg
     3601 aatatcatcc atatttaacc caacataaat tgaaattata tttggcagca caacgtatcg
     3661 tgatggaatc ttggtcacca ttgatgaatg cacaaatttt aaatgatgag acaattaaag
     3721 acattgctca agaattagga aagtcacctg cccaagttgt tttaagatgg aatgtgcagc
     3781 atgctgtggt tacaatccct aaatcggtga caccaaacag aatctctgaa aatttccaaa
     3841 tatttgattt cgaattatca gatgaacaaa tgacgcgaat tgatggttta aatcaagata
     3901 agagaattgg acctgatcca aaaaaatttg aaggctagat taaaatcgct caactgatga
     3961 aaaggttaga tgaattgt
//
LOCUS       DANRDI010000196         3974 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.198, whole genome shotgun sequence.
ACCESSION   DANRDI010000196 DANRDI010000000
VERSION     DANRDI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.198"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(429..578)
                     /gene="rpmG"
                     /locus_tag="PZ377_002554"
     CDS             complement(429..578)
                     /gene="rpmG"
                     /locus_tag="PZ377_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDM6359409.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(669..2186)
                     /locus_tag="PZ377_002555"
     CDS             complement(669..2186)
                     /locus_tag="PZ377_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDM6359410.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2390..3844
                     /locus_tag="PZ377_002556"
     CDS             2390..3844
                     /locus_tag="PZ377_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6359411.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT         1237 a          710 c          588 g         1439 t
ORIGIN      
        1 atcgtaatta ttacgattta tataataaca caatcctcac ataattcaac agtaaagttt
       61 catttctttg ttaaatcatt ttctaaacca tcatatttaa gattaaagtg tattatcgaa
      121 catttataaa acatacccag aattacttcg tacttattaa attccgcttt gaaattttaa
      181 attctactat attaaatact ctttcaagca ttcgctaaca aagtgacttt ataaaatcag
      241 taatctattt ccttctataa atattctcaa attttaataa agtaacaact aatataatca
      301 tatattgttc taccgatgaa actcaatcca ttaatcactc ttcacaaaaa tttcataaaa
      361 aagcgagctc cccaatataa taggtaagcc cgctttattt tcaaatcgtc gtatttgaat
      421 gataagaatt acttagtttc acgatgtaac gtatatttgt ttaatcttgg gcaatatttt
      481 ttcatttcaa tacgctcagg attattacgt ttatttttag tagtgatata gttacgatcg
      541 ccacattctg tgcatgctaa tgttacgttt acgcgcacgt taaacaccct ctcttgttag
      601 gaatctttac gatttattat gatatcataa actgctcaac tacgcaatat agaaactaca
      661 tatcaaattt atttttcaaa gttttcgtat gtttcatcat tttcagtttc aagatcaata
      721 gaatttatat caatacctaa tgcttttgca acacctttac catattctgg gtcagctttg
      781 taacaatgac gaatatgacg tcgtttaaca tcatccgtta cgccttccat tgcatttgct
      841 gtatttgtaa aaattctttc tttagcgtcc tctgattgta atctaaacaa tttacctggt
      901 tgttcaaaat aattatcatc atcttgacgt tgattatatt catagccgta tccatcagtt
      961 gggaatggtg gctttttata ttcaggttga gaatcaaatt taccatggtt atttggataa
     1021 taatgtgttc ctccaccttg gttattgtca actacgcgca tttgaccatc tctactaaaa
     1081 ggacaaatat tttcaatacc aacaccttta ggttggttta caggaatctg ccaatgatta
     1141 actcctaatc gatatctttg cgcatcgcca tatgagaata aacgcccttg cagcattttg
     1201 tctggagaaa aatctaatcc tggaataata ttagttggtg caaacgcagc ttgttcaaca
     1261 tccataaagt aattatctgg atttctattt aattcaaact ctccaacttc aattagagga
     1321 tactcatcgt gataccatac ttttgttaaa tcaaatggat tatctttatg gtttttagct
     1381 tgttcctcag tcattacttg aatatacatt gtccattttg gataatcacc tttttcaatg
     1441 gcttcgaata aatcgcgttg agatgaatca cgatctgtag ctataatttc agcagcttct
     1501 tcatcagtta agttttcaat accttgttgc gttctaaaat ggaatttaac ccaaacacgt
     1561 tcaccagaat cattatacat agagtatgtg tgagaaccga acccatgcat atgacgtaaa
     1621 tctttaggaa tccctctatc tgacattaag atcgttactt ggtgcaatgc ttctggaaga
     1681 cccgtccaga aatcccagtt attttgtgca tctctcatat ttgttctagg atctcgtttc
     1741 accgcacgat ttaaactaac aaataacttt ggatctctaa agaagaatac tggtgtgtta
     1801 ttccctacta aatcccagtt cccttcttca gtgtagaact ttaacgcaaa tcctcgaatg
     1861 tcacgctccg catcagcagc accacgttct cctgctacag tagagaaacg ggcaaacatt
     1921 tcggtttgct tacctatttc agagaatatt ttagcattcg tatattttgt tatatcttta
     1981 gttacagtaa atgtcccaaa tgcaccagaa cctttggcat gcattcgacg ttctggtatt
     2041 acttctctat caaattgaga catttgctct aaaaagtaaa tatcttgcat taaaagaggt
     2101 cccctaggcc ctgctgtcat actattttct cggtctgata ctggatgccc aaaaacacca
     2161 gttaactttt tgtcttgttg tgacatagtc atccctccac aatttataat aattataatt
     2221 taatacatat tataatttaa ccccatcaaa attacaatta aacatgctca agatgattaa
     2281 aaaatcagaa aattttaatt ttttcgcttt acagtactaa atagttattg aatagtacac
     2341 ttaaaaagct tacaataatg ggtatattta aatagatggg agactaaata tggaagataa
     2401 taaaatgaac cgtagtctta actcaagaca catttccatg attgctatag gtggtgcaat
     2461 tgggactggt ctatttgtag ctactggtaa tatcatttct caagctggtc ctggaggcgc
     2521 tatactcgct tatcttgtta ttggtgtcat gctatatttc ttaatgtcat caattggaga
     2581 gttggcaaca ttttatccag tatcaggttc attcagctct tattcaacac gctttattga
     2641 ctcatctctt ggctttacca tgggatggtt gtattgggca ttgtggtcat tagttacaag
     2701 tgttgatgtc atagtagcgt caaatgtgct ttatttctgg gacacattta aatttttcca
     2761 ccccattact tggagcttaa tctttattac aattttacta ttattaaaca ttttttctgt
     2821 aaaatcattt ggagaaactg agttttggtt atcattgatt aaagtgttaa caattatcgt
     2881 attcgttatt tttggctttt taatgatttt cggtatctta ggtggtcata catatggatt
     2941 tgaaaactat acaaaaggcc aagcaccgtt tgttggtggt atctctggtt tcttaggcgt
     3001 attattagtc gccggatttt cggttggtgg tacagaagta gtagcagtaa ctgctggtga
     3061 atcagatgac cctaaaaagt ctatgcctaa ggcaattaaa caagtatttt ggcgtattct
     3121 tttattctat gtcttatcaa ttgcagtaat tggtgcaatt attccgtaca cagatccatc
     3181 attattaaga gcaagtagtt caataagtca aagcccattt acaattgtat tcgatagagt
     3241 aggcatagcc tttgcagcat cagtaatcaa cgcggttatt ttaacttcat tattatccgc
     3301 tgcaaattca ggtgtttata caacaggcag aatgttgtat tccttaagtt cagacaaaaa
     3361 agcaccccaa tttttaagta aattaaacaa gacaactaag ttacctttaa gagcattatt
     3421 aactacttat gcagtcgttg ttattgttat tatttatgca aactttaatt caaatgccgt
     3481 ttttaattta cttgaaatta ttggttcaat gattatagtt gtttggggat caagcatttg
     3541 gtcacaaata cgattgcgac aagctattaa aaaacaaggt caagacccta ataaggtcct
     3601 accatataaa gcaccttttt atccattagg accaatcatt gtcatcacta cactattatt
     3661 cttgctattt ggtggctcag ttgaatatat tttaaaagat caatggttaa atgcttttaa
     3721 aaacttttta cctttaatca ttctagcgtt gatttacttt attcataaaa tcattcacaa
     3781 aacaaaattt gtaaagctag aaacaattaa tttaaaacca cacgattatg acaatcaaaa
     3841 ataaataaat tctcatacgg cagatgcttt agattatcta tagcatttgc cgctttttaa
     3901 tggtgtaaag cagatatact ttaatatctt tttctagtat aaagtgtatt aactagtatt
     3961 ctgtttatat ttta
//
LOCUS       DANRDI010000197         3897 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.199, whole genome shotgun sequence.
ACCESSION   DANRDI010000197 DANRDI010000000
VERSION     DANRDI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.199"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            176..1468
                     /gene="asnS"
                     /locus_tag="PZ377_002557"
     CDS             176..1468
                     /gene="asnS"
                     /locus_tag="PZ377_002557"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDM6359412.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            1796..2482
                     /locus_tag="PZ377_002558"
     CDS             1796..2482
                     /locus_tag="PZ377_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDM6359413.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            2472..3131
                     /gene="nth"
                     /locus_tag="PZ377_002559"
     CDS             2472..3131
                     /gene="nth"
                     /locus_tag="PZ377_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDM6359414.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            3136..3477
                     /locus_tag="PZ377_002560"
     CDS             3136..3477
                     /locus_tag="PZ377_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359415.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
BASE COUNT         1466 a          517 c          705 g         1209 t
ORIGIN      
        1 atatattttg tctgaaactg atgattgtat tgtcttaata taaattaatg ttcaaatctg
       61 tgcgtaaatc attaccattt acacgttatc ttttagtcta gttctagaac ttgaaagcgg
      121 attttgttaa aatgaatgag tattgtataa cgaaaaaata aataggagaa tggttatgaa
      181 aacaacgatt aaacaagcga aagatcattt aaaccaagac gttacaattg gtgcttggtt
      241 aacaaataaa cgttcaagtg gtaaaatcgc ctttttacaa ttacgtgatg gaacaggctt
      301 tatgcaaggc gtagtagtta aatcagaagt tgatgaagag gtattcaaac ttgcgaaaga
      361 aattactcaa gaatcatctc tatacgttac aggcacaatt acagaagata atcgttctga
      421 cttaggatac gaaatgcaag tgaaatcaat tgaagttatt tcagaagcgc atgactatcc
      481 gattacacct aaaaatcatg gtacagaatt cttaatggat caccgtcatt tatggttacg
      541 ttctaaaaaa caacatgctg taatgaaaat tagaaatgaa gttattcgtg caacgtatga
      601 atttttcaac aaagatggat ttacaaaggt tgatccacca attttgacag caagtgcgcc
      661 agaaggtaca agtgaattat tccatactaa atactttgat caagatgcgt ttttatctca
      721 aagtggtcag ttatacttag aagctgcagc aatggcacac ggaaaagtat tttcatttgg
      781 tccaactttc agagctgaaa aatcaaaaac acgtagacac ttgatcgagt tctggatgat
      841 tgaaggagaa atggctttca caaatcatgc tgaaagttta gaaattcaag aacaatatgt
      901 aacacatgtt gtaaaatcag ttttagaaaa ttgtaaacta gagttgaaaa ttttagagcg
      961 tgatacatca aaacttgaaa aagttgcgac accattccct agaatttcat atgatgatgc
     1021 aattgaattc ttaaaagcag aaggctttga tgatattgaa tggggcgaag attttggtgc
     1081 gccacatgaa acagccattg ctaatcatta tgatttaccg gtgtttatta ctaattatcc
     1141 aactaaaatt aagcctttct atatgcaacc aaatcctgaa aatgaagaaa ctgtattatg
     1201 tgcagactta attgcacctg aaggatacgg tgaaattatt ggtggatctg aacgtgtgga
     1261 tgacttagaa ttgttagaac aacgcgttaa agaacatgga ttagacgaag aagcatatag
     1321 ttactactta gacttacgtc gttatggtag tgtgccacac tgtggatttg gtttaggttt
     1381 agagcgtaca gtagcatgga tttctggtgt tgaacacgtt cgtgaaacag cgccattccc
     1441 aagattatta aaccgtttat atccataagt tttagggtcg ttacttaaaa tatatattat
     1501 gataataaaa agaatcgtcc agcctatatt aatagctggg cgatttattc ttggaaaatg
     1561 aagattgata acggcttata ttaatcatga acaaactttt gtttagtaac tatatttgga
     1621 aaataaatag ttcatctgat tatccataaa gttcattgtt aaaatctagt gttaaaaaat
     1681 actgttattt aaaatttgtt tgctactagt taaatatatt aagcatttta gtcgaaaatt
     1741 aaagaaaatg atttatacta ttaagtaatg gttaaattta aaagggggca cgaacatgga
     1801 taaatatcaa ttaaaagcaa gacctgtagt gatacgaaga gaattattag accattacag
     1861 tgacttaggt ttagatgagc aagatttagt cattttgctt aaattgattt atgcttccga
     1921 aacatcaaat aaacagcctt caattgaatt gcttcagaag ggctcaacta tgcaaccacg
     1981 tgatattaca atggttattc aaaatttgat acaacgtgaa ttgctagaat tacaagttca
     2041 aaaagatgaa gaaggtagat ttaccgaata tatgaatcta gatccttttt tcgaaaaatt
     2101 aagtcatata ttgaagcaac aaagtatgga aacgaaagag ctaaatagta aagaaaaatt
     2161 taaacaatta tttagagttt tagaggatac atttgcaagg ccgttatctc catatgaaat
     2221 agaaacgtta aatcaatgga ttgatgttga taaacacgat actgcaatta tacaagcggc
     2281 attagacgag gcgaatagtt tgaataaact tagctttaaa tatatagatc gtattttact
     2341 taattggaag aaaaacaatg tcaaaacaat tgacgattct agaaaaataa gagaaaaatt
     2401 caataaaccg aaaatgacgc acacagttaa aacggtacct aaatttgatt ggttaaacgg
     2461 ggagaacctt gatggtaagt aagaaaaaag cattagaaat gattgatgtt atagcaaata
     2521 tgtttccgga tgcagaatgt gaattaaaac atgataatcc gttcgaatta actattgctg
     2581 tattattgtc agcgcaatgt acagacgttc tagtaaatag agtaacaact gagctgttta
     2641 aaaaatacaa aacgcctgaa gattatttgg cagtcagtga tgaagaactt atgaatgata
     2701 ttcgttctat cggtctgtat cgtaacaaag ctaagaatat taagaaactt tgtcaatctt
     2761 tgattgatca atttaatgga gaaataccac aaacacataa ggaattagaa agtttagcag
     2821 gtgtaggacg taaaactgct aatgtagtca tgagtgtagc atttgatgaa ccttcattag
     2881 ctgttgatac gcatgtagaa cgtgtttcta aacgcttagg tattaatcgt tggaaagata
     2941 atgtgagaca ggtagaagac cgtttatgtt ctgtcatacc tagagataga tggaatagga
     3001 gccatcatca actcattttc tttggaagat accactgttt agcccgaaaa cctaaatgcg
     3061 atatttgtcc actattagaa gattgtagag aaggacaaaa acgttataaa gctagtttga
     3121 aagaagcgtg ataacatgac aaatattaca aaagaggtat ttgataattt agaacaagag
     3181 attgatttat ttgccaaaaa taagacatta ggttctagtg aagcaaagcc ctacttggat
     3241 gaataccata gtaaaattat tgattatttt aagcaggtta atgacataac tggcaatatt
     3301 gattttgata aattaaatca atatcctgtt gtgccaatga attttaaaga aagatatgat
     3361 tatatgattg aacgtaaata tcattttatg ggctatcgac aaatgaagac ctttaaaact
     3421 gaattgatta aaatgaatgc tagttatcaa acaaggttaa agaataagca ggtatagaca
     3481 agattgatca tttcctaatg ttgaaaatat ataaaactaa ttcatgcaca catatttata
     3541 attatgttag gtgtcgtgtc attacaacgt cttaatataa aaactgtcca tctacatcat
     3601 aatggatatc ttgagatatg ctttaagata tctatttcga atcgtaggtg gatttcttat
     3661 atctatgatt gtgaaaatat gttgcaaaca tgttgatatt tcactgatta acgtacacag
     3721 ggaattaata aatatatcaa tttaaaacag cgtctaagat gactatgaga aaatataatc
     3781 ataattttaa aaatgggaac aaccacttat tcaaattcaa taattttaca tagctaaaga
     3841 tgtgaatatg aaaaactatt tagatgtttc aaaatgtatg tgaatgactg attttac
//
LOCUS       DANRDI010000198         3870 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.200, whole genome shotgun sequence.
ACCESSION   DANRDI010000198 DANRDI010000000
VERSION     DANRDI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.200"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1047
                     /locus_tag="PZ377_002561"
     CDS             <1..1047
                     /locus_tag="PZ377_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6359416.1"
                     /translation="SIIIASILLWGVHFLILRGVETAAFINSIVTVAKLIPIFLVIIC
                     MIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIEGAVVFSGRA
                     KSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVLEHIVGHWGS
                     VLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPVNALIITNIL
                     VQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKANLKQWIIGII
                     ASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTLFIFIIVLAI
                     IGIVRLITGNISVF"
     gene            1089..1778
                     /locus_tag="PZ377_002562"
     CDS             1089..1778
                     /locus_tag="PZ377_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDM6359417.1"
                     /translation="MYEENIYIKNSEYEFDNNLKQLASYLNIPVSIVRPYKEDLTLYQ
                     YKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNYLRLSKDENIFPMNFIFNETPA
                     PYEICTALTDCKILTLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSA
                     KERIERILQILCLSIGDDNGEFYELKQIMTVQLISNLSGLNRKTTGEIIRELKIENII
                     YQDKRNWIIKK"
     gene            1816..2814
                     /gene="argF"
                     /locus_tag="PZ377_002563"
     CDS             1816..2814
                     /gene="argF"
                     /locus_tag="PZ377_002563"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDM6359418.1"
                     /translation="MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMK
                     GKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYD
                     GIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
                     VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDI
                     DEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPS
                     FHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            2834..3763
                     /gene="arcC"
                     /locus_tag="PZ377_002564"
     CDS             2834..3763
                     /gene="arcC"
                     /locus_tag="PZ377_002564"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDM6359419.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
BASE COUNT         1418 a          527 c          692 g         1233 t
ORIGIN      
        1 agtattatca tagcatcaat tttattatgg ggtgtacatt ttttaatact tagaggtgta
       61 gaaactgcag cgtttataaa tagtattgtt acagtagcta aattaatacc tatatttcta
      121 gttattatat gcatgatagt tgtattcaac ttcagtactt ttaaatccgg tttttatggt
      181 atgactagtg gaagtgttgg cgtttttagt tggggagata caatggcaca agtaaaaagt
      241 actatgttag taactgtatg ggtattcaca gggattgaag gagccgttgt cttttctgga
      301 cgtgcaaagt ctaaaaagga tgtaggaact gctaccgtta ttggtttgat ttctgtgcta
      361 gtcatttatt tcttaatgac tgtactagcc caaggtgtca ttcagcagaa ccaaatttca
      421 aaacttgcta atccatcaat ggcacaagta ttagaacata ttgtaggtca ttggggttca
      481 gtgttagtta atataggctt aattatctct gttttaggag cttggttagg atggacatta
      541 ctagctggtg aattaccatt cattgtagct aaagatggac ttttcccgaa atggtttgct
      601 aaagaaaata agaataaagc tccggtcaac gctttaatta ttactaatat attagttcag
      661 ttatttttaa ttagtatgtt gtttacagat agtgcctatc agtttgcgtt ttcacttgca
      721 tcaagtgcaa tcttaattcc atatacactc agtgcttttt accaggttaa atatactatt
      781 caaaataaat ctaaagctaa tttaaaacaa tggataatag gaattattgc atctatttac
      841 acaatttggt tggtttatgc agctggatta gattatttac tattaacgat gttgttatat
      901 atacctggat tactcgtata cagctacgta caaagggata ataacaaaca tttgacaaaa
      961 ttggattata cgttattcat attcatcatt gtacttgcaa taataggaat agttcgtttg
     1021 attacaggta atatttctgt attttaagtt atgatttaaa ctaagtaatt tttaaggggt
     1081 ttatagttat gtatgaagaa aatatttata ttaaaaattc agaatatgaa tttgataata
     1141 atcttaaaca attagcatca tacttaaata ttcctgttag tattgttaga ccttataaag
     1201 aggatttaac actttatcaa tataaaaaag gacaagtcat atatcattca actgatcaaa
     1261 taaaatttgt atacttttta gtaaatggtt gtattttaca tgaatcttct aatattactg
     1321 gtgacaatta tttaagatta agtaaagacg aaaatatatt tccaatgaac ttcatattta
     1381 atgaaacccc tgcaccatat gaaatatgta cagctttgac agattgtaaa atattaactt
     1441 taccgaaaga tttacttgag tatttatgta gaaagcataa tgaaatattt gaaagtctct
     1501 tcaagaaact taatgagact attcaatttc aagtagaata tattatggcg ttaagagcta
     1561 attcagctaa agaaagaatt gaaagaatac tacaaatttt atgcctttca attggggatg
     1621 ataatggaga attctatgaa ttaaaacaaa ttatgactgt tcaattaata agtaatttat
     1681 ctggacttaa cagaaaaact actggtgaaa taatcagaga attaaaaata gaaaatatta
     1741 tatatcaaga taaaagaaat tggattataa aaaaataaca tgaataaaat attaattgaa
     1801 aaggagatat tcaaaatgaa aaatttaaga aatagaaact ttttaacttt gttagacttc
     1861 acacaaaaag aaatggaatt tttacttaat ttatctgaag atcttaaacg cgcaaaatat
     1921 gcaggaatag aacaacaaaa aatgaaaggt aaaaatatcg ctctactttt tgaaaaagat
     1981 tcaacacgca ctcgatgtgc atttgaaaca gcggcttatg atcaaggtgc acatgtaaca
     2041 taccttgggc caacaggttc tcaaatgggt aaaaaagagt ctaccaaaga tactgctcgt
     2101 gttttaggtg gaatgtatga tggcatagaa tatcgtggat tctcacaaag agtagttgag
     2161 gatttggcaa aatattctgg cgttcccgta tggaatggtt taacagatga agatcatcct
     2221 acacaagtac ttgctgattt tttaacagct aaagaagtat tgaaaaaacc atataatgaa
     2281 attaacttca catatgttgg agatggacgt aataacgttg caaatgcctt aatgcaagga
     2341 gcagcaatca tgggcatgac tttccatctt gtatgtccta aagaactaaa tccaactgat
     2401 gaattattaa atcggtgtaa tgatatagct gacaaaaacg gtggtgaaat ccttataact
     2461 gatgatattg atgaaggtgt caaaggatct gatgtaattt acacagatgt ttgggtatct
     2521 atgggtgaac ctgatgaagt ttgggaaaaa cgtatcaaat tattagaacc ataccgtgta
     2581 actaaagaat taatgaaaaa aacaggtaat ccacatacaa tttttgaaca ttgcttacca
     2641 tcattccatg atactgaaac aataatcggt aaacaaattc aagaaaaata tggtctaact
     2701 gaaatggaag taacaaatga agtatttgaa agtgaacaat cagttgtttt ccaagaagca
     2761 gaaaatagag cacatacaat aaaagcagtc atggtagcaa cattaggaga gtaaatgaaa
     2821 ggaggatata aatatgtcta aaatcgtcgt agctttgggt ggtaacgctt taggacaatc
     2881 gcctaaagaa caattagatt tattaaaaag tacatctaaa tctctagtaa gtttaatcga
     2941 taaaggatac gaaattgtaa ttagtcatgg taatggtcca caagttggaa gtattaactt
     3001 aggtttaaat tatgcggcgg aacacaaaca aggtcctcct tttcctttcc ctgaatgtgg
     3061 tgctatgagt caagcttata ttggctatca gatgcaagaa agtttacaaa acgaacttca
     3121 ttcaatgggc atagataaac aagttgttac gctagtaaca caagttcaag ttgcaagcga
     3181 tgattctgct ttcaataacc ctactaaacc aattggatta ttttatacaa aagagcaggc
     3241 tgataaattt acgaaagaaa aaggttatac ttttgtagaa gattctggac gcggctatcg
     3301 tcgcgttgtc ccttccccac aacctataag tatagttgag ttagatagta tagaaacatt
     3361 aatcactcat ggaacactag ttatcgctgc cggcggtggt ggaattccag taattaagga
     3421 aaatgaagtt tatacaggtg ttgatgcagt tattgataaa gataaaacga gtgctttatt
     3481 agcagcacat ttacaatctg atcaattaat catattaact gctgtggacc atgtttacat
     3541 taactatgga aaagaaaacc aaagaggtct cgatgaagtg tctgtggatg aaatgaaaaa
     3601 acatatctct gatggtcaat ttgctaaagg aagtatgctt ccaaaagttg aagctgcact
     3661 tcaatttctt gaaaaaaata ctaaaggcag tgttttgatt acatctctag caggattagg
     3721 ggacgcttta gacggtaaaa taggaacatt aattaagaat tgaaattata atactttagt
     3781 tcaattacct ttataaacaa aagagcctat aatcctaggg cactgaaccc ttaaaacggg
     3841 aacttaataa aaacaccatg ttctaggcta
//
LOCUS       DANRDI010000199         3866 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.201, whole genome shotgun sequence.
ACCESSION   DANRDI010000199 DANRDI010000000
VERSION     DANRDI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.201"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(3..662)
                     /locus_tag="PZ377_002565"
     CDS             complement(3..662)
                     /locus_tag="PZ377_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359420.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(722..1864)
                     /locus_tag="PZ377_002566"
     CDS             complement(722..1864)
                     /locus_tag="PZ377_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDM6359421.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            2132..2518
                     /gene="gtxA"
                     /locus_tag="PZ377_002567"
     CDS             2132..2518
                     /gene="gtxA"
                     /locus_tag="PZ377_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDM6359422.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            2652..2759
                     /locus_tag="PZ377_002568"
     CDS             2652..2759
                     /locus_tag="PZ377_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDM6359423.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
     gene            3387..>3866
                     /locus_tag="PZ377_002569"
     CDS             3387..>3866
                     /locus_tag="PZ377_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359424.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     "
BASE COUNT         1209 a          619 c          662 g         1376 t
ORIGIN      
        1 aattaattag cgccaccatt gtcatcttta aataagcttt ctaatttaga tttaatactg
       61 tcgaattttg tattaatatc atttaacatg ctttcaattt taccttggct tgcattgttt
      121 ttaagtgcat tatttgcctc tgtcaatttg ttgatttggt cttgataaaa ttgagttagc
      181 ttagaatcag ctttgttttg ttcttgctgc ttcaagttgt cgatttgatt ttgtaaattt
      241 ttaataatgt catcagtttt ttggttgttt ttaatatttt ctttagcatt atctacttgc
      301 tggttaattt ggccgtcttg attttcaact tgttttttat aattttgaga gactttaggg
      361 tcattactaa ctttatcgct atcattgtta agtaaggcgc gtgtgacaaa aatagcgaca
      421 ataattaaga gaatggcaat aatgatatat aaccattttt ttgaacggcg ggatttctcg
      481 ttttcttctt caagaatttg ttctcttcga taatctctac cattatagta tcgttccggg
      541 tgttcttcag gatcgccttt agcatattgt tgtctatacg atgcgtcttc agattgacga
      601 cgatgatttt ggttttgatt gtcgtattga tatgaatcac ggtatttatc agttcttttc
      661 atgggtttca cctcattcaa gttttaactg caaataataa agctgatgat ttactttgac
      721 attattgtaa tttcagaata cgagcaatgt cagttgcagt gtttaataaa ttttgttcgc
      781 tattttgtag gacatcttct aaagtgctag gtgaagagat tatagaatag gcactatcaa
      841 taccgaaatc gtaaacatgt tgataatttt cgcctagact gccacaaatc gcgatgacag
      901 gaatatgata ttgttttgca gctaacgcaa cgcctacggg tgttttacca aagatggtct
      961 gataatccat gcgtccttct ccagtaataa cgaggtctgc atctttaatt ctttgatgaa
     1021 aatctgtaat gtcaaagacg acatcaatac cttttgttaa agttgtctca caaaacgcta
     1081 ataatgctgc gcccataccg ccagctgcac cagaacctgg tatttgatta acggactttc
     1141 ctgtgcacat ttttatctta tcatgataat gcgacattgc gaaatccaac tttggtatca
     1201 tctttgcatc agcgcctttt tgaggaccat aaatataggt agcaccattt tcacccaata
     1261 aaggatttga aacatcacag gccactttaa aggtcacctc ttttaatcgc gaatctagat
     1321 tggttatatc gatttgtgca atgtgagcaa gattagcacc attcatttgt aataagtccc
     1381 cgtttacatc agtaaacttt acgcctagtg cacttagcat acctgtacca ccatcatttg
     1441 ttgcactgcc accaatccct aaaataatgg tcttagcacc atgatttaat gcatctttaa
     1501 ttagttcacc ggtaccatat gatgatgtgt ataaaggatt acgttcctct ttttctaata
     1561 aatccaaacc tgacgctgcc gccatttcaa taattgcaat ttgttgttcg tctgcgcgtg
     1621 cataacatgc ttcaataggt cgcattaaag ggtcattaac gatgactgta tacttagtgg
     1681 cacctgttgc atgcattaaa gcatctgtgg taccttcacc accatcagcc atcggaatga
     1741 tatcataatg aagggtattc ccataaacat tagtaaaagc ctgttttata atattgccaa
     1801 cttgctgtgc ggtcatactt tccttaaatg agtcaggagc gatgacaatt ttttgtaatt
     1861 gcatagtaga catcctctca ttactgttgt cgctcttatt gtatataaaa ttactagtga
     1921 actaaagtaa tctctatagt gctaatgagt cacgcgataa atcagttaca ttataatggc
     1981 gtataaggat gaggggaaag agactaaatt tgctgtgaaa gggtataaag attgaaataa
     2041 gcatatttga agtgaagcgg attggctcgc atattcgagt atgttcgaga acgtagttat
     2101 gcatatattt tgatgttgag gagttacaca tatgaaacta acacaaacgc atgctgaaat
     2161 tttaaaattt atcattgttg gcggcattaa tacgttaaat tattatgttg tctatttatt
     2221 gctgttaaaa ttattacaca ttgaatatat gattagtcat attaccggat ttctagtcgc
     2281 ttttgtgatt tcatattatt tgaattgtta ttttgtttac agggtaaaac ctacttggag
     2341 aaaattcatt agttttccaa ttacgcagat tgtcaacgta agcttacaaa cagttttatt
     2401 atatgtattt gtatcctggc taaatttgcc agcagaaatt gcaccatttg ctggtttaat
     2461 tattacgata cccatcacat ttatattatc taaatggatt ttaaaagata gcaattaaag
     2521 tagcgtaaac cactaaaaaa aactttactt aagccttaat attgcgtgaa atataataaa
     2581 taaatgatat aatatgatgg tcgcctaaca tctcgggcgt tactttaacg cagagaggag
     2641 gtgtatgacg gatgttcaat ttattaatta acatcatgac ttcagctata agcggctgtc
     2701 ttgttgcgtt ttttgcacat tggttacgaa cgcgcaacaa taaaaaaggt gacaaataac
     2761 caacacctaa ataaatcccc tcactatggc agtagtgagg ggatttggtg tatttagatg
     2821 ttcaatttat tatgtaatta attatagcat tgatgaacaa ggtgtgcaat gtatttttgc
     2881 aatattacaa taaatgaatt taattatatt agaattaagt atttcgaaaa atgatttaat
     2941 agttgagtca taggatcatt agataaagcg aataatcaat tatagggaca ttgggataca
     3001 aatcaatgta ctagactcta caaggctata ttcagggacc caacaaagag aaattggatt
     3061 cccaatttct agagacaatg caagttgggg tgggacgacg aaataaattt tgtgaaaata
     3121 tcatttctgt cccactccca gaaccactac ataataaatc attagtggct ctttatcatt
     3181 tctgccccat aaccttgtga atttttaaaa gaaatgtgtc acaaatccac gcgacattga
     3241 taatcgttat caactaatat atgatgtact tgtaataagt attaattttt aacaaataac
     3301 aaatttatat tatgtatatc atccagtgaa ataaataata taagcggctt aatttagatt
     3361 attaattgaa aggagttaca tatacgatga acaatcagac aaattggata aagatactca
     3421 gcggatttgc ttctgatagt aaatggaaaa ttatgttatc aattttgttg tctatcatca
     3481 gtgtcttttc tggattagtt ccttattggg cagtatttaa aattatttta atgatgatta
     3541 acaatacata tacgattaat tcgattatgg tttatatctt tattgcttta atcgcttata
     3601 tttcacaagt gtgttgtttt ggagcgtcaa cgatgctatc acatattacg gcatatgaaa
     3661 ttttatctga aattcgtaaa aaattagctc aaaaattaat gcgtctccct ttaggcgtag
     3721 tggaatctaa gaaaataggt gaattaaaaa atatatttgt cgataaggtt gaaacaatag
     3781 aattaccttt agcacatatg attcctgaag ttataggaaa cttacttttg tcagctgcta
     3841 tctttttata cataatgctc attgat
//
LOCUS       DANRDI010000200         3758 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.202, whole genome shotgun sequence.
ACCESSION   DANRDI010000200 DANRDI010000000
VERSION     DANRDI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.202"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..323
                     /locus_tag="PZ377_002570"
     CDS             <1..323
                     /locus_tag="PZ377_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDM6359425.1"
                     /translation="NTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARP
                     IHRTPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKL
                     VINI"
     gene            630..2192
                     /locus_tag="PZ377_002571"
     CDS             630..2192
                     /locus_tag="PZ377_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDM6359426.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            2367..2903
                     /gene="amaP"
                     /locus_tag="PZ377_002572"
     CDS             2367..2903
                     /gene="amaP"
                     /locus_tag="PZ377_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDM6359427.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            2916..3155
                     /locus_tag="PZ377_002573"
     CDS             2916..3155
                     /locus_tag="PZ377_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDM6359428.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            3218..3727
                     /locus_tag="PZ377_002574"
     CDS             3218..3727
                     /locus_tag="PZ377_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDM6359429.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT         1289 a          533 c          687 g         1249 t
ORIGIN      
        1 acaatactat gattgaactg ataaaaccat tagggcatat tacgactatc gtagctttta
       61 atgaagatca agatttaaat gcattaaaat taaaaagtat aacatttaca catgaattta
      121 tgtttgcgcg tccaattcat cgaacgcctg atatgattaa acaacatgag tatttagaag
      181 atattacgaa aaatatagaa tcagggcatt accaaccaac aacgacacaa gtttttgaag
      241 gtttatcacc tgaaaactta tatcaagcac atcaattgtt ggaaaaacaa tcaatgattg
      301 gaaaattagt aattaatata tagattagta agtaaagtcg ccttatgcag aaaatactgt
      361 ataggcgact tattttttgt gtctagcgac tattgattct aaacggaaat atggcaactt
      421 tgcaggtatc aaatcaataa tgcactagaa aggcattact taaatacaat aaaatacttt
      481 gttgctcaat gataaataat ttgagtgtgt tttatatatc atttttaaaa gatgaggcat
      541 tattaattgt gtctatataa aattacaaat gattaaaatg ttgcagttat gggtatgaac
      601 ttaccattac acagaaaggg tttgtattta tgaaaaataa atcagtaagt ctagtgtttt
      661 gggtatcttt agtgatatgt acgatatttg ttgcttttgg agcaatattt ccgaaacagt
      721 tagaaaaact cacgcaaaac attactacat ttattgcgct acatttttct tggtattatt
      781 tattgctcgt tttagttatt ttatttgtct gtgtgtatat tttattttcg agatacgcaa
      841 acattacatt aggtgaagaa ggcgaagatc ctgaattctc attgccgtct tggtttgcta
      901 tgttatttag tgctggtatg ggaataggac ttgtgttctg gacaacggca gaaccaatca
      961 gtcatgcctt caaattaacg cctattcata aagctggcac gcaatctgca attaatgatg
     1021 caatgcagtt ttcatttttc cattggggga ttcatgcatg ggcagtctat ggtattgtcg
     1081 cgctagtgtt tgcatatttt agctttcata aaggatatcc tggcttagtc agcgctacat
     1141 tgacgccttt attaggtgaa aaggcaatgc gtggaccttt aggtggtgcc attgatgtat
     1201 tggcagtcat agctacagta acaggcgttg ctgcaacatt aggtttcggt gcattgcaaa
     1261 taaacgaagg tttacatttt ttatttaatg taccgagtaa cttcacgatg caagtaatct
     1321 tgattgtcat tgcaaccata ttatttactt ggtcagcatg gtcaggtatc gataaaggta
     1381 tcaaaacgtt gagtaatatc aacatgttac ttgcttttgt agtacttatt ggtcttttta
     1441 ttgttgggcc aacgctatac attttaaata catttacgaa tggtctaggt aattatattg
     1501 caaatttctt tagtatgagt ttacgcattc cgacaggtgg tcaaaaattc caatggttac
     1561 aaaattggac aatcttttac tgggcatggt ggatatcttg ggcaccattt gtaggtatat
     1621 ttattgcacg tgtgtcaaaa ggacgtacga ttaaagaatt tattctaggt gtactttttg
     1681 tcccggcgtt agtttgcttt atattctttg cggtattcgg cgcgtcagca atttacttac
     1741 aagataatca tattgccgat attgctaaag ctgcgacaga aacagcaact tttgcgacgc
     1801 tacaacatta tccgctaggt tttgtattaa gcttaattac tttacttgtt attatgattt
     1861 tctttgttac atcagcggat tcggcaactt atgttttagg tatgttgagt tcaagtggtg
     1921 atattaatcc gaagtcattt gtcaaagtga gttggggtat cattatggca ttatttgcaa
     1981 tcatcatgat ttacacaggt ggtactcaag caattcaaaa tttattaatc attgctgcgt
     2041 taccattttc cgtggtcatt atagcgatga tatggtcgct tataaaatct ttgagtgagg
     2101 agaaaccgag aaatagtaat aaggttttaa ttaaacatcg tgacccagat attttagaat
     2161 atcgttcttc aaaacatatt gacgaaaatt aattaataat gaggttttat ttacaaaaac
     2221 tagggaatat attactaaat ggtacttcat cttatatatt aaaaataaat ttaaagttat
     2281 taatttatta tgtttaagaa aaaaactgat gggtacatat ttaatatgtt tttaaattaa
     2341 ctttaactaa ggaggtgtcg ttaggggtga aacgacttaa aaactttatc cttggcttat
     2401 taatagtagt aatcgtgggg ttcctgttat tcatgtatat ccaagatgga cgtataactg
     2461 aatatcaaga ctacttcctt caatttgaat ggttccaacc attacttatt tcacttgctg
     2521 cactattgat attaataggt cttatattag tatttagtat cttcaaacct acgcatcgaa
     2581 aacctggatt atacaaagac ttcgatgatg gtcatgttta cgtatctcgt aaagctgttg
     2641 aaaagacggc attcgatacg gttgcaaaat atgatcaagt aagacaacca aatgtagttg
     2701 cgaaactata taacaaaaaa aataaatctt atatcgacat taaaactgac tttttcgtac
     2761 caaataatgt tcaagttcaa tctttaactg aagcaattcg ttcggatatt aaaaagaatg
     2821 tagaatattt cacagaaatg ccagtacgta agttagaagt taatgtgaga gatcagaaaa
     2881 cttctggtcc acgagtgttg taagggaggt atatcatggc taacaatcat aaccaaaacg
     2941 gacaagactc tacacaacaa gtgattaatt ttttaaaagt atttaaatgg agaatcgttg
     3001 gtttcctagc gtttctatta atcgcgatat tattcttaac gttaggtttt tggaaaacag
     3061 tacttatcat cgttttatgt ttaattggtg taggtattgg gtatatgaaa gaccgtaagc
     3121 aagattttat gaatttttta aatcgatgga gttaattcat caatttagaa tttaattaaa
     3181 aaaatataaa aattaacaag taaaaggaga atctattatg actgtagata acaataaagc
     3241 aaaacaagca tacgacaatc aaactggtgt taacgaaaaa gaaagagaag agcgtcaaaa
     3301 acaacaagaa caaaatcagg agcctcaatt caaaaacaaa ttaacattct ctgatgaagt
     3361 tgttgaaaaa attgctggta tcgctgcacg tgaagttaaa ggtatcttag atatgaaagg
     3421 tggcttaact gatacattca ctaatgcatt ctcaagtggc aacaatgtta ctcaaggtgt
     3481 atctgttgaa gttggtgaaa aacaagctgc tgtagactta aaagtaatct tagaatatgg
     3541 tgaatcagca ccaaaaatct tccgtaaagt aactgaatta gttaaagaac aagttaaata
     3601 tattactggt ttagatgtag ttgaagttaa catgcaagtt gacgatgtaa tgactcaaaa
     3661 agagtggaaa caaaaacacg aaaaaaataa cgaaaacaat aaccaagaaa gacaaggttt
     3721 acaataagtt attcgataaa aagggcttgg gacattaa
//
LOCUS       DANRDI010000201         3714 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.203, whole genome shotgun sequence.
ACCESSION   DANRDI010000201 DANRDI010000000
VERSION     DANRDI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.203"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..652)
                     /gene="mreC"
                     /locus_tag="PZ377_002575"
     CDS             complement(<1..652)
                     /gene="mreC"
                     /locus_tag="PZ377_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDM6359430.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVV"
     gene            complement(852..971)
                     /locus_tag="PZ377_002576"
     CDS             complement(852..971)
                     /locus_tag="PZ377_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359431.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            complement(1045..1518)
                     /locus_tag="PZ377_002577"
     CDS             complement(1045..1518)
                     /locus_tag="PZ377_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDM6359432.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            1678..1875
                     /locus_tag="PZ377_002578"
     CDS             1678..1875
                     /locus_tag="PZ377_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359433.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            complement(1992..2276)
                     /locus_tag="PZ377_002579"
     CDS             complement(1992..2276)
                     /locus_tag="PZ377_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359434.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(2344..3030)
                     /gene="radC"
                     /locus_tag="PZ377_002580"
     CDS             complement(2344..3030)
                     /gene="radC"
                     /locus_tag="PZ377_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDM6359435.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            complement(3027..>3714)
                     /locus_tag="PZ377_002581"
     CDS             complement(3027..>3714)
                     /locus_tag="PZ377_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDM6359436.1"
                     /translation="SCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLKWYELMPI
                     ISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTYVFLLIFT
                     MTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIGYGDVLLI
                     SALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFINSLFYND
                     IHQFLGGVYF"
BASE COUNT         1269 a          623 c          495 g         1327 t
ORIGIN      
        1 taacgacttt atcacctttt gagatattat ctctattatt aatgtcacta attacaagtt
       61 ctgagttctt ttcatcataa cgatcaatta aaccaaatat atttttagaa ccgtgttgta
      121 tatttacaga taatttaccc gcacgtgtat tagttgagat taaatcaact tgtgaagaaa
      181 atttattaac tttagtaact cttccaacaa aaccttgtga tgtcatcaca gccatatttg
      241 aagttatacc tgatttagat cccttatcaa ttacaattgt attcatccac tgatccggat
      301 ttcttgccaa aaccgtagta gaaataggat caaattttga aatatctttt aaatcaagct
      361 ctttttttaa tttttcattt tccgcttcta attgttggtt cttagattct aactggctaa
      421 tcttattttt agattcttta gaatctcctt ttttaaaaaa gtccccaatc gtaccagcaa
      481 caaaattaac tggataactc acaactcgtt gtccaaaaga cacagaatca cctatatatt
      541 gttcaggagg tgattgagat tgtgaacgta tggacagccc aattaatgca ataaaaacga
      601 taattgcaca taaaacaaca attaatttgt tatttttaaa aaacttaagc acccagaaca
      661 cctctattat gtcaaaatat tgtatatcct tttctatttt atattactcc cattatgaaa
      721 taaagcacta actacgaatt actttgaaaa tgaacaactc cgtaaaaaat ttgtggcatt
      781 ttaaaaacaa aaaaactact agcaaacatt ccagtcacta gtagtttaaa taatcgattt
      841 aaattttaaa attatttgtt tgtatcgttc gaggattcac tgttcgaatt ctgaagttgt
      901 ctccaacgtg cgaataagtt ctgggccttt tgttgttcat tagcttgttt ctgttttgat
      961 tgttctgcca tttgaactac accaaccttt taaatttatt ctagtgacag gataactaaa
     1021 atatatttct tatgcaataa tttcctattg ataagcattt tcagatttta gttgtaaatt
     1081 ttgccctaat tcatttaagt cttgttgcat ttcaaattca gtattgtaaa cacgcattga
     1141 ctcatctcca aatttataaa gaataaattc gtctcctctt tgacctataa tatattgatc
     1201 attataagcc atgcgattca ttccagacac agccataaac tcttgtttat ctatcatttt
     1261 aaatacattt ttaatttggc ttaatggtac attctttatt aaatcatttt cttttaatga
     1321 atatttctca ccacttacat aaacatgttg ggcatcatta gtgggttgcg taacttgatt
     1381 catttgatca ttatcgtgat ttacaggatt ccaatcttgt tcttttaaaa aaggtgcgta
     1441 tttcaaggcg atataaacaa ttatcacaat tgctattact gctataatgt ttttaatcat
     1501 actaaatatc aatctcatag ttcgcctcca atgtcttcat ctaattatat attttattat
     1561 gtatattttc ctcagtcttc agccttaatc atgatatatc ttaggactga tgcacgttca
     1621 tttaaaatag cttataattt tgttatgatt taaaagcgta ttaggaggtt tgtagcattg
     1681 agtatactaa caattatttt aatagcacta ttagtcatcc tcctttttag agttggactt
     1741 tctattttgc gatttttaat ttatgtcgga cttgttttat tatgtattta cttaggatat
     1801 caaggtttaa tttggttgct cgattttttt caaataaata gtggattttt accgcacttt
     1861 caatttaaca attaaattta ttctgcataa caaaactcct aatgtactag tttagaattt
     1921 tttaactagt tccacattag gagttttagt ttgttttcaa gcacggatac tacttgatga
     1981 acagattact attacttacg cccaaatgcg ttagataaaa atcgtatcag cgcgtagcta
     2041 ccacttagta aaaggcccca aacgattcca gtaaaaattg agccaattgc tacacttaat
     2101 aaaggaaaag cttgtttcgt aacgattaca cctgctaacg cggttactaa aatacctaac
     2161 gtagataaag ctaatgttat taaaagattg cgctttgtta caggttcatt tttaatattt
     2221 aactgcatgg tataaaccat tggcacgaga accccgataa taataaatag agtcactatt
     2281 tttcctccaa tgaaagtact tcgtaggtat tatacacaag atacgtcatt aattctatca
     2341 acttcaatca ttttcatcaa agtaacccgc ttctacaaga ctggtaaatc tattatcacc
     2401 gattataata tgatccaata aatctatccc taaaatcaaa ccacactcct tcaacctcat
     2461 tgttgttatg atatcttctt gtgagggcgt tacatcaccg gatggatgat tatgaactgc
     2521 gatgattgca ttggcatttt ctctcaccgc aatactaaaa atttcacgtg gatgtacaat
     2581 cgaactattt aatgtacctt taaaaacaca ggtttcttta atcactacat tttttgaatt
     2641 taacaataaa atgacaaaat gttcttgtgt taaatctttc attgttggaa tcatataatc
     2701 agcaacatca cttggttgcg ttatttttat acgattattt tcagctcttc tccccatcct
     2761 ttcccctaac tcaaatgctg cttttaaagt aattgctttt tgtaatccaa tccctttaac
     2821 ttttatcaaa tcgttaattg aagatttttt caattcattc agattcgaag cagatttaag
     2881 cagttcatta ctaatgtcta tgctcgagaa tccttttctt ccggtgttaa ttaatatagc
     2941 taataattct gtattcgaaa gactttttgc accatggctt aacaaacgtt ctcttggcat
     3001 ttctgaagtt accatttctt taattttcaa aaatatacgc ctcctaaaaa ttgatggata
     3061 tcattataaa aaagtgaatt gataaaaaag gaaataaata taaatggaac aaggggtaat
     3121 agtttaatcg gcttaaatat catggtaatt aaagcaacta aaccagcaat gacaaatgta
     3181 aataaaatga cataaatagt gaattggaga gggaaaaaca aagaaagtgc agatattagt
     3241 aaaacgtcac cataaccaat atatgcccga aataaaaagt agaatatatg cgtggtcata
     3301 ctaataatga taaaagctac tggataaatc atacttaacg agagagaaac gatacaataa
     3361 attataatta agcgacaatc taacattaaa gaagtgatat cggtcatagt aaaaataagc
     3421 agaaaaacat atgtagttat aaatagcgta gcatttacgt atgtgaaatc atacttaata
     3481 aagacgatag gtattaaagc aaaggtttcc cctaagaaat gtgttaggga aatacgcttt
     3541 cgacagtttc gacatcgccc ttttaataat aaaaaactaa taatcggcat taattcatac
     3601 catttgagtg acgaattaca ataatcacat ttcgatcttc tatgtaaata atcaaatgac
     3661 gtttcttcta tagatataaa ttgatataga aaactaaaaa tacaactgca acta
//
LOCUS       DANRDI010000202         3695 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.204, whole genome shotgun sequence.
ACCESSION   DANRDI010000202 DANRDI010000000
VERSION     DANRDI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.204"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..235)
                     /locus_tag="PZ377_002582"
     CDS             complement(<1..235)
                     /locus_tag="PZ377_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359437.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDA"
     gene            549..1235
                     /gene="mtnN"
                     /locus_tag="PZ377_002583"
     CDS             549..1235
                     /gene="mtnN"
                     /locus_tag="PZ377_002583"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDM6359438.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            1255..1782
                     /locus_tag="PZ377_002584"
     CDS             1255..1782
                     /locus_tag="PZ377_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDM6359439.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1783..2883
                     /gene="yqeH"
                     /locus_tag="PZ377_002585"
     CDS             1783..2883
                     /gene="yqeH"
                     /locus_tag="PZ377_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDM6359440.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            2897..>3695
                     /gene="aroE"
                     /locus_tag="PZ377_002586"
     CDS             2897..>3695
                     /gene="aroE"
                     /locus_tag="PZ377_002586"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDM6359441.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLK"
BASE COUNT         1335 a          469 c          709 g         1182 t
ORIGIN      
        1 atgcatcgaa tataattttc ttgatgtttt tatcaacaac atcaggtaag gacataagta
       61 taaatcctag aattagagtg tagcgacata tgttcataat tgttgctgta aactcgatat
      121 taaatgaaaa ctttaaaaat atacttataa ataaaatgac aaacgacgca attgcaataa
      181 aactgccgta tgtagctatc tttgttacga gactattttg attattttca gccattgtaa
      241 aatcctctta tttcatcatt attattttaa acatatcatt ttcctgttat ttaataaagt
      301 ttcttgacac gattggttga tgtaattgat atgattagtt tttgaaaaat agacaaatta
      361 gaagcatcga attcactgtt atgagttcaa aaataattat ttatgatacc aagttattga
      421 aagagctttt tataaaaata aagcaaaagt tttatacagt aatgaaaatc atgatacttg
      481 tcgttgtagt gattaaaatg tgataaaata acaaatgcta ggatttttta aaagaggtga
      541 atcaaaagat gattggtata attggtgcca tggaagaaga agtaacaata ttaaaaaata
      601 aattaacaca attaagcgaa atttcagttg cacatgttaa attttatact ggcattttaa
      661 aagatagaga agtagtgatt acccaaagtg gcattggaaa agttaatgct gcaatttcta
      721 cgacattatt aattaataag tttaaaccgg acgtcattat taatacaggt tctgctggag
      781 ctttagatga aagtttaaat gtaggtgacg ttcttataag tgatgatgta aaatatcatg
      841 atgcagacgc aacagcattt ggttatgaat atggacaaat accacagatg ccggtagcat
      901 ttcaatcaag taaaccttta atagaaaaag tatctcaagt tgtacaacaa caacaattaa
      961 cagctaaagt aggcttaatt gtaagtggtg atagctttat cggtagtgtt gaacaacgcc
     1021 aaaaaattaa aaaagcattt ccaaatgcga tggcggttga aatggaagca actgcaattg
     1081 cacaaacatg ttatcaattt aatgtaccat ttgttgtagt tcgtgcagtt tcagacttag
     1141 caaatggaga agcggaaatg agcttcgaag catttttaga aaaagcagct gtatcatcaa
     1201 gtcaaactgt tgaagcatta gtgtctcaat tataaaaagg taaaggtgag ttaaatgggt
     1261 ttagttcgca agttttttat gccgaattca tatgttcaat caatatttca aattgattta
     1321 gacaagttag tggacaaagg cgttaaaggt attattacag atttagataa tacgctagta
     1381 ggttgggatg ttaaagaacc tacagaacgt gttaaagcat ggtttaagga agctaatgaa
     1441 aaaggaatca ctattacaat cgtgtctaat aataatgagt ctcgtgttgc tagttttagt
     1501 cagcatttag acatcgattt tatttttaaa gcgagaaagc caatggggaa agcgtttgat
     1561 aaagcaataa ctaagatgaa tatcagacca gatcaaactg ttgttatagg tgaccaaatg
     1621 cttactgatg tatttggtgg taatcgtcga ggtctatata caattatggt tgttccagtt
     1681 aaacgaactg atggctttat tactaagttt aatagattaa ttgaaaggcg attattacgt
     1741 catttcagta aaaaaggtta tatcacatgg gaggaaaatt gattgtctga cattttaaaa
     1801 tgtatcggtt gtggtgcgcc acttcaatct gaagataaaa ataaacctgg ttttgtacca
     1861 gagcataata tgtttcgtga tgacgtgatt tgcagacgtt gtttccgctt gaaaaattat
     1921 aacgaagttc aagatgtagg attagaaagt gaagactttt taaaattatt atcaggactt
     1981 gcggataaaa agggtattgt cgtcaatgtc gtggatgtat ttgactttga aggatcattt
     2041 attaatgcag ttaaacgtat tgtcggaaac aaaaaaatca ttttagcagc aaataaattg
     2101 gatttattgc cgaaacaaat taataaacgt cgagttaaag aatggttaaa acgaacagca
     2161 agaaaatatg gtttggaagc tgacgatgtc gtattaattt cagctgaaaa aggctggggc
     2221 atagacgact tattatcatc aattgcgaat attcgagaaa atgaagatgt gtatattgta
     2281 gggacaacga atgttgggaa atctacattg attaataaac tgattgaagc tagtgttggt
     2341 gaaaaagatg tagtaacaac ttcaagattc cctggaacaa ctttagatat gatagatatt
     2401 cctttagatg aaacatcatt tatgtatgat acaccaggta ttattcaaga tcaccaaatg
     2461 acgcatttag ttagtgaaaa agaattgaaa attattatgc ctaagaaaga aataaaacaa
     2521 cgcgtatatc aattaaatga ggcgcagaca ttattcttcg gcggtctagc gcgcatagat
     2581 tatgtatcag gtggtaaacg tccgttagtt tgtttctttt ctaatgactt gaatatacat
     2641 cgtactaaaa cggagaaggc taatgattta tggcgtaatc aacttggcga tttattaacg
     2701 ccacctggaa atccacaaaa ttttgatctt aatgaggtaa aggctgttag acttgaaaca
     2761 ggcaaagaga aacgcgatgt tatgatctct ggtctaggct ttataactat aggaccaggg
     2821 gctaaagtaa tcgttcgtgt tcctaaaaat gttgatgttg tattaagaaa ttctatttta
     2881 taaggtgatt aaaaaaatga aatttgcagt tataggaaat cctatttcac attccttgtc
     2941 gcccgttatg catagagcaa attttaattc tttaggatta gatgatactt atgaagcttt
     3001 aaatattcca attgaagatt ttcatttaat taaagaaatt atttcgaaaa aagaattaga
     3061 aggctttaat atcacaattc ctcataaaga acgtatcata ccgtatttag attatgttga
     3121 tgaacaagcg attaatgcag gtgcagttaa cactgttttg ataaaagatg gcaagtggat
     3181 agggtataat acagatggta ttggttatgt taaaggattg cacagcgttt atccagattt
     3241 agaaaatgca tacattttaa ttttgggcgc aggtggtgca agtaaaggta ttgcttatga
     3301 attagcaaaa tttgtaaagc ccaaattaac tgttgcgaat agaacgatgg ctcgttttga
     3361 atcttggaat ttaaatataa accaaatttc attagcagat gctgaaaagt atttagctga
     3421 attcgatatc gttattaata caacaccagc gggtatggct ggaaataacg aaagtattat
     3481 taatttaaag catctttctc ccaatacttt aatgagtgat attgtttata taccgtataa
     3541 aacacctatt ttagaggaag cagagcgcaa gggaaaccat atttataatg gcttagatat
     3601 gtttgtttac cagggtgcgg aaagctttaa aatttggact aataaagatg ctgatattaa
     3661 ttctatgaaa acagcagttt tacaacaatt aaaag
//
LOCUS       DANRDI010000203         3668 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.205, whole genome shotgun sequence.
ACCESSION   DANRDI010000203 DANRDI010000000
VERSION     DANRDI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.205"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            34..612
                     /locus_tag="PZ377_002587"
     CDS             34..612
                     /locus_tag="PZ377_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020362254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDM6359442.1"
                     /translation="MQKIFTQELRFKDENGEEYPEWENKFIKDIFIFENNRRKPITSS
                     LREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNH
                     AHVVKSNDHNLFFMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLTEQDKVSA
                     LLKSIDNKMNNQMNRIELLKERKKGLLQKMFI"
     gene            1462..>3668
                     /locus_tag="PZ377_002588"
     CDS             1462..>3668
                     /locus_tag="PZ377_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDM6359443.1"
                     /translation="MKRSTNQEKFLDTLIRLNTKIEELGKINILNNHIYSEYFFRDLL
                     NIVYGYSLENHNKKQKNAPAFDLIDNTNKIIIQVTATCKKQKIEDTLKKEYLTNKMEE
                     GYRLKFIFIGNQNNNIKNKNFSNPHNILFDSKKDIILTQDLCEEFLNLNINKQDHAIE
                     LLKKELSPLLFEDSLSYLKEEFINEKLEFNISNLASRYTANNDVDTINNKIIEGISIT
                     NNFKYTNISYLKELKGYIENDILDKMKSKYTKNIYLNFKKIFSNLEQSVNNYLELEEE
                     FEEKKKYLSEIYELIDEINIDPYIFLTEHNECNIYKISENEKLELQTYMSKIEKVLLK
                     YQTYLKETCKECLFYPYLLVQGEAGIGKSHLLAHLSKKLRDENHIIYLFLGQFFTKNE
                     DPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDALNEGEGKRLWGNYFQSFI
                     NHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEGFSKEENYNPIVSFCDFY
                     GLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQTISVAELFTNVLKTVNINLSKE
                     DKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEESYTVVNNIAKEYVQKSNRFLEAL
                     IDENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEKYRNVDKRDLVTKLQSDEKVTR
                     YFQKEDDLSYNRGLINELFIKLANEFNIELFEVFPQFKNNYNMIYSFINSLVW"
BASE COUNT         1549 a          391 c          529 g         1199 t
ORIGIN      
        1 cttgaattgc ttcaacaaca gaaaaaaggc tatatgcaga aaatcttcac acaagaattg
       61 cgattcaaag atgagaatgg tgaagaatat ccagagtggg aaaacaaatt cataaaagac
      121 atctttatct ttgaaaataa tagaagaaaa ccaattactt cttcattaag agaaaagggg
      181 ttataccctt actatggtgc aactggaatt attgattacg taaaagatta tttattcaat
      241 aatgaagaac gattactaat aggagaagat ggtgcaaaat gggggcagtt tgagacgagt
      301 agctttattg ctaatgggca atactgggta aataatcatg cgcatgtagt taaaagtaat
      361 gatcataatt tgttttttat gaattattat ttaaatttta aagaactacg agcatttgtc
      421 acaggtaatg caccagctaa attaactcat gcgaacttat gcaatataaa tcttaaaata
      481 ccttgtctca ctgaacaaga taaagtaagt gcattgttaa aatctataga caataaaatg
      541 aataatcaaa tgaatagaat tgagttatta aaagaacgta aaaaaggact attacaaaaa
      601 atgtttattt aaccctgata acaacctatt aattgaaatg ataaaaatcc tatgaataaa
      661 ttgtaatata ttaatattga gactattata atttggggtt tattttttta gaagtttagt
      721 gaatcgtcta catttagttg gacaaattct atgagaatag acattgttaa actaagaggg
      781 taggcgattt tattatgaca agagactgaa gagcttttag taaataattt aaattaaaat
      841 aattaaactt tatgaaaatg gtaagcttag aaacgaaatt atacgagaat atgatctaac
      901 atcattaact ttctcaaatt agataaaaca aaaccaaaat actgagtcat tcaatcatca
      961 agacatttta acaagtgatg aaaaagagtt aataaaatta cgcaaagaaa aacatcgatt
     1021 gataatgtaa aatgatgatt taaagcaagc agtgctaatt ttgagacaaa aaatgaagtc
     1081 tttcaacaaa atgcgtatca atattcagta tcagcaatgt gcaaagttct aaaaatttca
     1141 agaagtacct attatgattc taaaaaagga gaatattaca actagataca aaaagcctca
     1201 atcacaacca agtaactaaa acgtaaaaat taattagatt aattattatc tgcataacaa
     1261 atgaccatat acatcaacta gagacagact atttaatcat ttacaatgtc ctattcgttt
     1321 tagaccatgt tcaaatgaat agtgtttata aaatgttgac actgtagtgg catattgtaa
     1381 attaaatatt attatgtgat cattttgttt aaatattagt tataagaagt gaaatattca
     1441 ttttaaaagg agacttaaaa cttgaaacga tcaacgaatc aagaaaaatt tcttgataca
     1501 ctaattagac ttaatactaa gattgaagaa ctaggaaaga taaatatttt aaataatcat
     1561 atatattccg aatatttttt tagagacctg ttaaatattg tatatgggta ttcacttgag
     1621 aatcataata aaaaacaaaa aaatgcacca gcatttgatc ttatagataa tacgaataaa
     1681 attattattc aagttaccgc aacgtgtaaa aagcaaaaaa tagaggacac tctaaaaaaa
     1741 gagtatttaa caaataaaat ggaagaaggt tatcgattaa agtttatatt tattggaaat
     1801 caaaataata atattaaaaa taagaatttt tcaaatccac ataatatatt attcgatagt
     1861 aaaaaagata ttattttaac tcaagactta tgcgaagagt ttttgaattt aaacataaat
     1921 aagcaagatc acgctattga attattaaaa aaagaattat ctccgctttt atttgaagat
     1981 tctttgtcat atttaaaaga agaatttatt aatgaaaaat tagaatttaa tatatcgaat
     2041 ttagcttcac gatatactgc gaataatgat gtcgatacta ttaataataa aattatagaa
     2101 ggtatttcta ttaccaataa ttttaaatat actaatattt catatctaaa agaattaaaa
     2161 ggttatatcg aaaatgatat tttagataag atgaaatcaa aatacacaaa gaatatttat
     2221 ttgaatttca aaaaaatatt ctctaattta gaacaatcag tcaataatta tttggaactg
     2281 gaagaagagt ttgaagaaaa gaaaaaatac ttaagtgaaa tttatgaatt gatagacgaa
     2341 attaatattg acccttatat ttttctgaca gaacataatg aatgtaatat atacaaaata
     2401 agtgagaatg aaaaattaga acttcaaact tatatgagta aaatcgaaaa agtattatta
     2461 aaatatcaaa cgtatttaaa agaaacatgt aaagaatgct tgttttatcc ttatttgcta
     2521 gttcaaggtg aagctggcat aggaaaatca caccttttag cgcatctatc taaaaaactt
     2581 agagatgaaa atcatattat ttatctgttt ttgggacaat tttttactaa aaatgaagat
     2641 ccatggcatc aaatacttaa tgatttagaa gttacaaatt ctgttgataa ttttttaagg
     2701 tcaataagta ataaggccaa agaaacaaaa aaaagagctt ttattattat tgatgcgctt
     2761 aatgaaggtg aaggtaaaag gttatgggga aattattttc aaagctttat caaccatata
     2821 aagaagtatt ctaatatagc tttaatattt tctattagaa ccccttttga agatgttata
     2881 ttacccaaaa acgcaataca agataacaat attgtagtat ttcagcatga aggttttagt
     2941 aaggaagaaa actataatcc aattgtatct ttttgtgatt tttatggatt agagctacct
     3001 aagttaccta tattaaatcc agaattcaac aatccattat ttttaaaact aatgtgtgag
     3061 tactgtgtaa ataaatttaa ggaatttgac caaactataa gtgttgcaga gctatttaca
     3121 aatgttctta agacagtaaa tattaattta tctaaggaag ataaatttga ttttgataaa
     3181 aatattaatg ttgtacaaaa agtaataaaa ggattagttg aattaatgaa cgactcagaa
     3241 tttaatcaac taaattatga ggaatcctat actgttgtta ataatatagc taaagagtat
     3301 gttcaaaagt caaatagatt tttagaagcg ctaatagatg aaaatatatt aattaaaaat
     3361 actggctata aaggtgaaat gattatttat ttttcgtatg agcgtatggg agattacttt
     3421 ttatcagaat accttttaga gaaatataga aatgttgata aaagagattt agtaactaaa
     3481 ttacagtcag atgaaaaagt gacaaggtat tttcaaaagg aagacgattt atcttataat
     3541 agaggtctta ttaatgaact ttttattaaa ttagcaaatg aatttaatat agagctattt
     3601 gaagtatttc cacagtttaa aaacaattat aacatgatat attcttttat aaatagttta
     3661 gtatggag
//
LOCUS       DANRDI010000204         3655 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.206, whole genome shotgun sequence.
ACCESSION   DANRDI010000204 DANRDI010000000
VERSION     DANRDI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.206"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            404..616
                     /locus_tag="PZ377_002589"
     CDS             404..616
                     /locus_tag="PZ377_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359444.1"
                     /translation="MNQFKEIYNTIEKLLNDKSISNYRINQDTDVSYGGISELRSGKR
                     KVNNLTLETAEKLYNYQKQLEIMIED"
     gene            910..1116
                     /locus_tag="PZ377_002590"
     CDS             910..1116
                     /locus_tag="PZ377_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359445.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
     gene            1817..1927
                     /locus_tag="PZ377_002591"
     CDS             1817..1927
                     /locus_tag="PZ377_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359446.1"
                     /translation="MKMTIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
     gene            2355..2540
                     /locus_tag="PZ377_002592"
     CDS             2355..2540
                     /locus_tag="PZ377_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359447.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            3052..3156
                     /locus_tag="PZ377_002593"
     CDS             3052..3156
                     /locus_tag="PZ377_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359448.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            3285..3536
                     /locus_tag="PZ377_002594"
     CDS             3285..3536
                     /locus_tag="PZ377_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359449.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
BASE COUNT         1534 a          359 c          562 g         1200 t
ORIGIN      
        1 ttgaaaaata taggctatta tactttagaa ttacaaatta taaatcttgc cagtggatat
       61 gggagttcaa catgtcactt accccaattt gtggaatgtt aaataactat aaagaggaaa
      121 ggagaaaaaa tgataagaat ggttaaagaa aaagaacgaa tagcaaaatt aaatttcgaa
      181 tagctgtgac gagcttaata attcaagtac ttaattttat caaatcgttc ttttaaaaag
      241 gtaaagggca aaagaccttt accacattaa atagggaggt ggtatttatg acaagagaat
      301 taaggaaaaa attaactctc tatcttaaca ttgcaacttt aatattattt attattaatt
      361 taactagaaa aaaataaatt attctttcat tttaacaaaa attatgaacc agtttaaaga
      421 aatttataat acaatagaaa aattactaaa tgataaatca atatctaatt atagaattaa
      481 tcaagacact gatgtttctt atggtggtat aagtgaatta agaagcggga aaagaaaagt
      541 gaataattta actttagaaa cagcggaaaa actctataat taccaaaaac aattagaaat
      601 aatgattgaa gattaaaata aggccatatt tttgaaatat catggagtga gccttttaag
      661 tcatctaatt ttaaagtata ttaaatatgg ataacttaat gtgaatataa ataaaaaatt
      721 atcaaataat ataatataga atagatggag acaagaaatt ttatcaatat ataaaaaaaa
      781 cgccaatact attaggtatt agtttatgaa ttaactagtt tggcaactag aaaaaaacaa
      841 tgtattgcta aacgtagtta ctgcattagg attattataa ttataaatag aaagagagtg
      901 tgattaataa tgaatgagat tgaaactatt ataagtgaaa tagaaaagtt attaactaac
      961 aatacaccat atagtatttc aaaaaactca ggtgtaccac gtcaaacagt tactgattta
     1021 aaggtaggta aaactaaaat aaaagaagct aaatttaaaa cgataatcaa gttatatgaa
     1081 tatcaaagaa cattagaaaa taaaacagaa tgttaacaaa aatattttgt taggttaggt
     1141 ggacaagaaa ttaaatttct aatgatttca tggttgtctg tccacgtttt gtccacctca
     1201 ataagataat ctcttagttt tagaaattgt acatggttaa ataatcaaaa atgcatatta
     1261 tgaaattatc attgccatta aatcttgaat gatctttaag cgatgatata aaaggctgaa
     1321 cacagtaaat aagttaactt taatgcagta ttctatacta tacatatttt gtaataatgt
     1381 ttatgcacat aatttttcat tgacaatttt ttatattaaa ttgtgtaatt ggcatatatg
     1441 atgatttgtg ataattatat tgtaatatag catttggttt tatgattcta aaattaatag
     1501 atttgaaaag tatactttat ttcatgaaag tttttggtac atttatttga aaatgttttt
     1561 attacaaagt tacatggcta aatgtttctt aacaaaatta atttttcgag tgtaaagaat
     1621 cgtgtggtaa acataattta tatagattat aaggaggtga aaatggtcga ggaagttaaa
     1681 acgataaaat agtttgaatg ttttgatttc aattctggta ggactattga atttgcaatc
     1741 taataagaat gtgaggtttg atttaatatg aagtcatatt ctttgaataa aatattactt
     1801 tctcaccatt agtaaaatga agatgactat taaagtgcga aaaacaaata aaaaagagaa
     1861 aatagaattt ttaataggga cgtttataat tattttagta atattagggt tcaaaattat
     1921 gaaataaatg tatagtattt gagaagtcta tatgactccg gaaaaattaa attctaaaga
     1981 tatctcaata tctcaaattt aatagactac tggagttagt agtttgaata ttgaaagaca
     2041 ctaatctaat aaaaatacat aaaaggttaa actttagaag tggctgtaaa cttcaatgaa
     2101 tactaaacat tgaagttttg taaaaggcgt aaggtataca agacaaatcg tttatctttg
     2161 acaaagtaat tgatgatgaa aagtcgttaa tatgtagaaa caaaaattga aaatataaaa
     2221 tttaactatt atgaagtgat gatagccatt aatgaataga aaaataataa aatcatttat
     2281 agataaaaaa tacgttaagt tcacagaatt tcgaaaaaaa gcagaaaaca ttgtacagat
     2341 atggagatga aaaaatgtca gaatacaaga aaaaaataat tgaattaatt gaaagtaatt
     2401 taacaggata tgaaatttct aaaaaaactg gagtttctca atacgtactt tcacaattaa
     2461 gacagggcaa acgcgaagta gataatctaa ccctgaatac aacagaaaaa ttatatgaat
     2521 atgccaataa agttttgtaa tttaactaat gtataaatta atcaagctat gtttatttga
     2581 tttaactatt aataaaaatc atatggtgaa tggatattat aataattaaa atacaaaaat
     2641 agtagattcc aatttgtgaa aaaggagtat gtcgtttcat ttagaattta ttgcacgaaa
     2701 ttagcatcga ataagggaaa ataagtgtta agttttaaat gataaaaaag attggaatgg
     2761 atcgtcttga aatgctccct tcaaagtttt cattttttca atgtcgactt cgaagggggc
     2821 attttcatta aattgttata gctttttata tttgtataat gaacatataa gtttaagaag
     2881 gtgcgagtga aggaaataaa aaagctcaaa tgtaccaaat tgttaatctt aataaatctc
     2941 tactttataa agattgaatg gacattcgag cgttaatcag tcaggaggga ctttccctcc
     3001 tacaatttaa taataatact tgcttcacca ctatacaagg agtgagttgt tatgttcaaa
     3061 gtgaattatt cgattttaag ttattatcca gaatataata tcgcagtaag ttggcaacgt
     3121 ttaagagaag gaaaaacaat aaaaaacaag atttaatact gctgcgtcat gaggcgcttg
     3181 aacattattt gatgaataag tataatttca actatgatta tgcacataaa attgtatcaa
     3241 aaaaatacga ttattcaatt tttataaaaa agaaggtgga ttaaatgctt acattaataa
     3301 aattgaaaga agatgaacag gttataatat atgaatatat acctgaagat gatataagta
     3361 acggtaaagg ttcagtaact tttaataaaa aagatgcaga ggttatagat ttctcattat
     3421 ctgaaataga aaatgaagaa tattttatgt tatatcgtaa taagtctttt tctgtagtaa
     3481 gagactttat cgagaagcaa gaatttcccg aaaattataa aatagcatgg tattaaagca
     3541 tcctttctac acagataaag agaaagcgat gctattttta tatgcttttt tcattattcg
     3601 ttgaaaaaga agagtcacaa aagttaacat atttaaaaga acttggtgaa gatgg
//
LOCUS       DANRDI010000205         3639 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.207, whole genome shotgun sequence.
ACCESSION   DANRDI010000205 DANRDI010000000
VERSION     DANRDI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.207"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(115..1206)
                     /locus_tag="PZ377_002595"
     CDS             complement(115..1206)
                     /locus_tag="PZ377_002595"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDM6359450.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            1371..2402
                     /locus_tag="PZ377_002596"
     CDS             1371..2402
                     /locus_tag="PZ377_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDM6359451.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            2894..>3639
                     /locus_tag="PZ377_002597"
     CDS             2894..>3639
                     /locus_tag="PZ377_002597"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDM6359452.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQN"
BASE COUNT         1322 a          542 c          626 g         1149 t
ORIGIN      
        1 tataactgtg attcgtataa aataagtctc taaagataaa tatttcatat atcacaatag
       61 attttcacaa taatatctaa gaatacatgg aatttatcaa aagagactta ataattattg
      121 gatataacaa tcaaaatcac tcaatgcttg cataccgcgt tctcggtcag tagggttttt
      181 gaaactaatt tttaaagcac cgtatatatc ttcgcgtact tctaagattc ttaagttgct
      241 tatagatatg ttatgtaaac tcaggatata agtcacttta cttatcatac ctgattcatc
      301 cggaatgtct acatatagat catacgcagt atttagtcca cctagttgtt tagcgggtag
      361 tgcgtcgcga tacgatttag cttgggcaaa aaatgataac aatttttcag aatcattgct
      421 ttcaattagt ctttctaaat cttgaaactg actttttagc tgtcgaatca tttctaaaat
      481 atacgtttta ttactcaagg tgatatcttt ccacatttgt gcattactac tagctatacg
      541 agtgatatca cgaaaaccac cagctgcaag tttattaact aaatgatgtt cttgaccgtt
      601 cttttgacta acatgaacta aactagatgc aacgatatga ggtaaatgac ttacgacgct
      661 tgttacgtag tcgtgttctt cagcagtagt tacaataaat ttagcaagag taggtgataa
      721 cagttctttt aacgtgtttg ctgcttgctc atttcttggc tcattgtaga ctaaaatata
      781 ataagcgttt tcaaataagt gctttttagc atttagtaca ccagatttat gactaccagc
      841 cattggatga ccactgacta aatgaatatt atgctttaat aaattgcatt cgtgttgctg
      901 tatcattgct ttagtactac cagtatcaga aacaataaca ccaggtttag ttggcatatc
      961 tataagctcg ctaagatatt tatttgtgat agcaacaggt gttgcataaa taattacatc
     1021 ggctttttta atagcttcac tataatttaa acatttttca ttaataatgc cgattgattt
     1081 agctttatct aactgagaag tatctgcatc gtatgcaata atattagtat tagggttatg
     1141 gtattttata ttgctagcaa gacttccacc aattaatcca agcccaacaa ataaaactgt
     1201 tgtcatataa atcaccttat ttcgaaattt tcagaataat aacattgtaa atgagctgtt
     1261 gacacagtgc aatagtaaat aaaaatcgat aatagcatta atagataaac ggagataaat
     1321 catctacaat aaagagtata gtaacacaat ggcaacggag gggtaaatca atggaaccaa
     1381 tattagaaat gattaaaaca ttaacaggta ttaatagtcc ttcaggagac acagaagaag
     1441 caattcaatt tgtcgaaaaa tatgcaaaag acttgggtta tcaaacaaca ctaacaaata
     1501 aaggtgcgtt attaataaca gtgccaggca aaaatgatga agtacaacgc tgtattactg
     1561 ctcatgttga tactttaggt gcaatggtta aagaaattaa agaagatggt cgcttagcaa
     1621 tagaattaat tggaggattc acgtataacg cgattgaggg tgaatattgc caaattaaaa
     1681 ctgatgctgg tcaaatatat acaggaacaa tttgtctgca tgaaacaagt gttcatgtat
     1741 atagaaataa tcatgaaata cctagagatc aaaagcatat ggaaataaga attgatgaag
     1801 taactacatc agaagaagat acaaagagtt taggtatttc agtaggtaat tttgttagct
     1861 ttgatccacg tacagttatc acgtcatcag gttttattaa atctcgtcat ttagatgata
     1921 aagctagcgt agcgatgata ctacaattac taaagaaatt aaaagaagag caaataatat
     1981 taccacatac aacgcaattt tatatttcta ataacgaaga aataggttac ggtgcaaatg
     2041 catcaattga ttcgaaaatc aaagaatata ttgcattaga tatgggcgcg ttgggagacg
     2101 gtcaagcatc ggatgaatat acagtttcta tttgtgccaa agatgcttca ggtccatatc
     2161 ataagcaatt gaaatcgcac ctagttaatc tttgcaaaat aaataacatt ccatataaag
     2221 tagacatata tccatattat ggttcagatg cttcagcagc tttacatgct ggtgcggata
     2281 tcagacatgg tttatttggc gctggcattg aatcatctca tgcaatggaa cgaacacata
     2341 ttgattctat taaagcgaca gagaaattac tatatgcata ttgcttatca ccaattgagt
     2401 aaacaattag tgttgacaaa tgtgaacgac ctatgtaata taatgaacta taaaaataat
     2461 tagaattttc taaagaaata gtagcagata tgaaacgtag caaatagaaa gctaatgggt
     2521 gatgggaatt agcacgccat atcttgtgaa ttggactttg gaaaacaatt gaatgagttt
     2581 tgaaagtgaa catgaattat gttaactaag gtggcaccac ggtaacgcgt ccttacaggt
     2641 atatgcgtta tgtggtgtct ttttatttag acaaaatgta gtagttaatt aaaggtagca
     2701 acagaaagtt agtggatgat gtgaactaac accgagatta atgaaattgg gttttgtctg
     2761 caacagaaaa attatatata gtaaagagtg aactatgaat atttcgaata ttcggttaat
     2821 ttaggtggta ccacgcgtca gcgtccttta tattgataag gatgctggcg cttttttgaa
     2881 aggagcgtat agaatggata tattttataa aaaaataaaa gcaaatgtaa cgcccgaagt
     2941 tttagcacaa cttcattcca agaagatcat tttggaaagt acaaatcaac aacaaactaa
     3001 aggtcgctat tcagttgtta tttttgatat ttatggcact ttaactttag ataatgatgt
     3061 attatcagta agtactttaa aagaatcgta tcaaatcact gaaagaccgt accattattt
     3121 aacgactaaa ataaatgaag actaccataa tattcaagat gagcaactta agtcattacc
     3181 atttatatct ggatatgttg ggacgtgtag ctttgattta gtaagacatg aatttcctaa
     3241 attgcaatca atacaattag aagatcacaa gcagcacgat gtaaggttat atatggttga
     3301 acaagtttat gtatttgacc attacaaaga tgagttatat atcatcgcga cgaatcaatt
     3361 ttcaaattca acaaaatcag atcttgagaa tcgagttatt aagtctatcg aagacttaac
     3421 taaaatccaa ccattcatgc ctacacaaga ttttgatttt aaaactaaag aaattcaatc
     3481 aaacatttct gaagaaagat ttatcgaaat gattcagtat ttcaaagaga aaataacaga
     3541 aggggatatg ttccaagttg tgccatcaag aatttacaaa tatgcgcatc atgctagtca
     3601 gcatttaaat caactttcgt ttcaactgta tcaaaattt
//
LOCUS       DANRDI010000206         3616 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.208, whole genome shotgun sequence.
ACCESSION   DANRDI010000206 DANRDI010000000
VERSION     DANRDI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.208"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..270)
                     /locus_tag="PZ377_002598"
     CDS             complement(<1..270)
                     /locus_tag="PZ377_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDM6359453.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANK"
     gene            complement(394..1095)
                     /locus_tag="PZ377_002599"
     CDS             complement(394..1095)
                     /locus_tag="PZ377_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359454.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(1088..1861)
                     /locus_tag="PZ377_002600"
     CDS             complement(1088..1861)
                     /locus_tag="PZ377_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359455.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(1848..2678)
                     /locus_tag="PZ377_002601"
     CDS             complement(1848..2678)
                     /locus_tag="PZ377_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6359456.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(2671..>3616)
                     /locus_tag="PZ377_002602"
     CDS             complement(2671..>3616)
                     /locus_tag="PZ377_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDM6359457.1"
                     /translation="IVVLFVISTLTFILMKLSPGNPVDKILHLDVAQVSTEQINATKD
                     KLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPITLLISFSTLVVSLC
                     ISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIVTNLMNIDSVILSQF
                     ILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERYILIHDLLKPTILPI
                     IPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQGCVLFIGFFVVIIN
                     TIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
BASE COUNT         1259 a          635 c          513 g         1209 t
ORIGIN      
        1 tttatttgct gcttctggac tagctgtaaa gagtaatgca atcatatgtt tacatatcac
       61 tcgtcttcca tcagcatgag gacaattaca tatggatttt ctaggatgtt ccatatcaat
      121 ataacaacga tatactttgt tgccactgcc ctttacttca gcctcatgct gcgtttctga
      181 aaatgatttt aagttaatga cgcattcact ttgataataa ttaaagcctc tttctataga
      241 acgaatactt gcaatatcaa gtaatcccat taatgatact cctttttatt attattttta
      301 aataaagaaa ataaaataga taagtgtcta gattaaaata cttgatttat ctatatttta
      361 taacaagtct agaattatcg cattcttaaa taactaatat gaaaatgctt gcactaattc
      421 ttttgtataa gggtgtctat caacattaaa taattcctct attgcaaaat catcgactat
      481 catgccatcc ttaagaacga taattctatt aactaagcgt tgtaacacgg ataaatcatg
      541 agaaataacg ataaaatgat ttaagttcgt aatcgtttgc gcttttaata tattgattac
      601 attttgttca gctataacat ctaaatttga agttatctca tcacatatta aaacgcgagg
      661 ctgtgctaat aacgaacgca tgacattaaa tctttgtaat tgtccgccac tcacttcgct
      721 tggtaattta gtcaataatt gcgcgtttaa ctcaaaagta gataaatgtt gtaataataa
      781 ttgatcctga gcagtattat cagttagacc tctgtaataa tataacgctt cttttaatga
      841 ggtctcaatc gtccaatcag ggttaaagct agttaaaggg tgttggaaaa tcggtaacac
      901 agcattgtca cttaagtaaa tctctccttt aacaggttta aacaagccaa gaaccaatga
      961 agcgagcgta cttttaccac agccactttc gcctaaaata ccaacatttt ctccatcagg
     1021 tatagtaata ttgatatctt gtagcaccat ctgcttttta ttataaccaa aagtcacatg
     1081 ttttaactca atcatagtaa tccctcttta attgtgttct atatttaatt agacgttcag
     1141 tatacggatg caaatgctca tacttgaaat gattaatatt acctcgttca atgatttgac
     1201 cttcttttaa aacataaatg tactgacaat atttcaatac atgacttaag ttatgtgtga
     1261 taataaataa tgtttgacca tgttctaata caatatgctg taataaatcc atcacttgat
     1321 taccgttcaa agcatccaat gatgcaactg gttcgtctgc aatgattaat ttaggctcca
     1381 acatgagaac gcttgctatg tatacgcgtt caagttggcc cccagaaagt tggaaactat
     1441 atttatttaa tatatctttg ctttgtaaat taacccacga caaagcctta tcaactttgg
     1501 acaaagcctc ttctttacta cctttataat gcttacgata aatcgcagtt aactgtttac
     1561 ctaatttagt atggtcgtta aaactttctg cataattttg agaaatatag ccaattgtat
     1621 gaccataata ttgactcaat ctactaacat tttccccatc aaattggtac gaatcatacg
     1681 tgcagcttaa atcaaatggt aaatattcaa gtaaagcttt agcaatcaaa ctttttccag
     1741 cgccgctctc tccaatcaag gcattaatct gttgactaaa aattttcaaa tcaatccctt
     1801 taataagaga tttctcacta gtattcttta ttgttaaatt ttgtatatca atgagactca
     1861 tcatattcac cccgttgttt cagcaatcta tctcttagtg catcaccggt taaattaaaa
     1921 attaaaatag ttatagcaat gactgaagca ggtgcaatca acataattgg atgagacgaa
     1981 ataaaatcac gaccttgttg caacatagcg ccccactctg gtgttggcgg ttgtgcacct
     2041 aacccaataa atgatagtga acttatatat agaatgattt taccgaaatc aacgaccatc
     2101 aaaacgataa tagccggtat aattttaggt gttaaatgac gtattaatat tgttcttgtt
     2161 ggtacatgaa ataattgtgc catttttata taaggcttat tcatttcgct attaactata
     2221 cttctagtca accttgtgta attcatccat tttattaatg taattgagat aactaaattc
     2281 cataaagatg gttgaaaaaa acttgctaaa gcaatcatga tgataaattc tggaatactt
     2341 agaccaacat caataaacct taacactaat cgttcaatcc accctttttt gtatccggca
     2401 aatagaccta gtgtaacacc tatgacaacg atagctatta atgttaaaac agtaacaaac
     2461 aatgttgaac gtgcaccgat aataattcgg gtaaataaat ctctcccata atcatcagtt
     2521 cctaataaat gcaaccaact aataggttca aaagtttgtg ataaattgac tttggttgca
     2581 ttttcactac tgacaaagaa ttgcagtaca attaccacaa aaataaatgc aacgaataca
     2641 aaaaatatca ggttattctt tgaaaatatt ttatgcatga cggtcactac tttctgatat
     2701 caatggtgta ttggttttga tttttggatt tcctaattgt aaacgctgct tcggatcaag
     2761 taataacgtt aataaatcag caatcgtatt gataataaca acgaagaagc caataaataa
     2821 cacgcatcct tgaataacag gataatctcg agatttaata ctatccatta atagataacc
     2881 aataccaggt atatcaaata aattttcaat cactacagta ccacctatta gactgccaag
     2941 tgaaatccct agtaatggga taatcggcaa aattgttggt tttagtaaat catgaattaa
     3001 aatataacgt tcattcatac cgcgtaatct tgatgcttgt acgatattac tttgcaataa
     3061 catcaataaa ttagaacgca ctaaacgaat gatgtatgca cacataccta aagatagcgt
     3121 gattacaggt aatataaact gacttagtat aacgctatct atattcatta aatttgtgac
     3181 aataaataat aaaataatac cgataaagaa cgctggtaaa ctaatcgata gtgttgagat
     3241 cactctaatc actttatccg tccacttatg aaatcgtttg gctgctataa tgccgagcgg
     3301 tatagatatg cataacgaca ctactaatgt tgaaaatgat atgagtaatg ttatgggtgc
     3361 atagttgaat aatatctgtg ttaccggttc ttttgattca aaactttttc ctaaattaaa
     3421 atgtaataaa tgattcatcc aatgccacca ctgtaccaat aaagaatcat ttaatcccaa
     3481 tttatctttg gttgcattta tttgttccgt cgacacttgt gctacatcaa gatgtaatat
     3541 tttatcaaca ggattgcctg gtgataattt cattaaaatg aatgtaagtg tagaaataac
     3601 aaataaaaca actatc
//
LOCUS       DANRDI010000207         3604 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.209, whole genome shotgun sequence.
ACCESSION   DANRDI010000207 DANRDI010000000
VERSION     DANRDI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.209"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(235..795)
                     /locus_tag="PZ377_002603"
     CDS             complement(235..795)
                     /locus_tag="PZ377_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDM6359458.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(963..2870)
                     /gene="sasF"
                     /locus_tag="PZ377_002604"
     CDS             complement(963..2870)
                     /gene="sasF"
                     /locus_tag="PZ377_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDM6359459.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(3112..>3604)
                     /locus_tag="PZ377_002605"
     CDS             complement(3112..>3604)
                     /locus_tag="PZ377_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDM6359460.1"
                     /translation="ILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANV
                     HLYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTL
                     SEHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQA
                     LES"
BASE COUNT         1026 a          663 c          498 g         1417 t
ORIGIN      
        1 taaacaagga gttcggactt aaacatattt ctgttcataa gtctgatttc ttattcaatt
       61 aaacccgagg cattcagttc gaacgcctcg ggtcgtttta tataaatata ttattttatg
      121 ttcaaatgtt cctcatcata tccgtttcaa ttgccatctc acacatttta taaatatgag
      181 caaatgtact tattctcaaa cattactgcc gagctttaat tgacgttata ttaactataa
      241 actacttttc catgactcta cggattcaat gtcacatgag cgtgataaaa tttgttcaat
      301 aataaagtca tgtttatcat ctgatctatc accaacagca tcttctaaaa cagtaatata
      361 atagtcttta tctacacttt ctaatgccgt gctcaataca gctccacttg tagagacacc
      421 cgttaatact aaatgattaa tatcatttgc acgtaaataa acttccaagt aactacctgt
      481 aaatgcgcta aagcgtcgct tagaaataat cggctcatct tctagtggtg ctaaatcttc
      541 aagtattcgt gtagatgcat ctgcttcagt aatcgcatat ccttgagctt taattgttga
      601 aaacacttta ttactcgagg agacatcatt aaaatgctta tctaacacta aacgtatgaa
      661 aatgactggt attcgatgtt gtcttgctgc ttcaattgct ctctgattcg ctttaataat
      721 attttttatt ctaggtacac tactcgctat accttcttgc atatccaaaa ctaatagcgc
      781 cgtttttcga gacatcttca ttctccttta cttgtgtagt tctaagtcgt taaattcatt
      841 ataacgttaa aatgatggac aatctattca ttgcattttg catatacttc acaataaatt
      901 aaggggaaat aagacgtctt atatacttaa aaaaatatat agatgctctt cccccaatat
      961 aattatgctt tatttttcaa cttattgcgt cgtgataacc aaatcattag tacacccatt
     1021 gcaccaacaa ttacagatat cggcaaccaa tgttctttta tcgtttcccc cgctttaggc
     1081 aaagatacat taccatcagc atttaataat ccacttaaca atccattacc tttaccaagt
     1141 gttaacgtct tttctggctt tggtgtgggt atatctggaa tactgtctaa taaatctgat
     1201 ccttgattca ttaaatttgc taacttattt aaatctgtcg ttttcccatt tttattcaat
     1261 cgatctagta aacttggacg atttactatt ggtgataaaa tataatctat atcttttttc
     1321 gtttgattga gtctcttttg taaattcaat aaatcatccg ctttaccatt caatgccgat
     1381 ttaactaaat taaaaatttt attttgatct gtttctattt tagtaattaa atctgccagt
     1441 aattttgcct tttgtctttc tatacgtgtt gctaaaatcg tttcaattgc ttgtttttta
     1501 tctttggcat tattcaaaat tgcttttaat atatcatctg aagacgtgtc gccagttgat
     1561 gcaaaatgtt tcttcaattg gtcaacgatt tggcgatttg ataatccttt attcgtccaa
     1621 tctttagcca atttatctgc ttcagctttt cctaatttcg tttgtaaaat ttgagaaatc
     1681 aatagcgact tatcttgtga ttgatcaatc aatgacgtta ataaatcatc actcgttgtc
     1741 agagataatt gatcaatatg acgagtaatt tgatctgcaa tttgttgatc tgttttacca
     1801 tcaacacgta tatcttttag aattttaact gcctcgtctt tattaaatat actttctaaa
     1861 atgctttgtg tagcatactt tttatcatca gtacgtgcaa gttcttccaa aataatattt
     1921 cgttgacttt ttatacgctc tttcgtctta tttacttcgc tcattaagtc tgatttttga
     1981 tttttaggaa gttgcgtatt tgcaatacgt tgatctaaag attgtaacgt attcagttta
     2041 tgatatgtgt aatgttgcgt tgaggcatta cttttagcta atttttcaat catagcatga
     2101 ttaattttat cgcttccttg taatttatca gtgagtcgat tactatggct ttgattctct
     2161 tcatttgaaa ggaatttatt taacacaaca tgtccagaac catcattatt tggcgtttta
     2221 gctacttcat gattattatc tgttgtagac actgctggat cttccgatgt atctttcgat
     2281 gcatctttcg atttgtgtat ttgctgattc aaatggtcta ggtcttctaa cgccttattt
     2341 accattgctt catcattttt atcatctttt tctccatgtt ttgttgtagc cgtttgtgac
     2401 atatcatttt tcattgcatt aagatcgtcc tcgccacttt gttgacccct atcaacattt
     2461 gaagaaacct catttaaatc tttaagcaat tgatctaatt tactgtctat atcactttga
     2521 ccgttcattt cagtgtgaga acttttattg tctttgctat ccaactcatt agctcgtttt
     2581 atgatttcat ctatttgcga tgctgttttc gcttcattta gttgtgcttt ataatgttct
     2641 ttagatgaag ccgataactg ttttaattgc tcaatttgac gaattgcctt gtcaactttg
     2701 tctaataaat cttgcttaga taatatctct tttgtaattt cagtatcctt ttcagatgca
     2761 gcttgggcat cgtacggcaa gatattcgtt aaaatgatac ttgtcgccat cattgtcgaa
     2821 cacgataact ttacatataa ttgaaacggt ttccctcgat atttagccat caacatactc
     2881 cttcctcact tacttccttc aaagaattac atactattat atacctgttt acaagaaatt
     2941 tacacttatc tatctagtta ttgttgttag taattatcta cttattactt agcttatatt
     3001 taagtaaaca aaacaagcat gacgtaatat catattgtcc atgtcgctaa catcatatta
     3061 cgtcaaatct tttatattaa atgatgtttt attttagact gctttttcct tttagctttc
     3121 gagcgcctgt ttaaaaactt gctcgaattg ttcacgcgag atttcgtgtg catgtgcttt
     3181 ttgtgctaat aaagcatctc gaaactgttg ttgatctttc aaactttcta acatttgtat
     3241 taattggtct ttactttcca ttgttatctc atcattatgc tcaaataagt gctctgataa
     3301 tgttacttta gcatggtgtg cggtttgacg ataacctaaa atcaacaact catagtcaaa
     3361 cgcttgttcc accgcattta aaatttcatt accctcattg atatcaagat aaatatcaca
     3421 taactggtat agttcattta ccctgtcaat attaatagat gggtataaat gcacattagc
     3481 atattgatca agttgcatta gcttatcaga catctctgta atagcagcga tgtgaaactt
     3541 aaaatctggt aaagtttcaa ccaatacctt gatgttacga agttgatccg agttagttaa
     3601 tatt
//
LOCUS       DANRDI010000208         3548 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.210, whole genome shotgun sequence.
ACCESSION   DANRDI010000208 DANRDI010000000
VERSION     DANRDI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.210"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(201..905)
                     /gene="budA"
                     /locus_tag="PZ377_002606"
     CDS             complement(201..905)
                     /gene="budA"
                     /locus_tag="PZ377_002606"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDM6359461.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(942..2606)
                     /gene="alsS"
                     /locus_tag="PZ377_002607"
     CDS             complement(942..2606)
                     /gene="alsS"
                     /locus_tag="PZ377_002607"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDM6359462.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQVEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            2839..3078
                     /locus_tag="PZ377_002608"
     CDS             2839..3078
                     /locus_tag="PZ377_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359463.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            complement(3060..3242)
                     /locus_tag="PZ377_002609"
     CDS             complement(3060..3242)
                     /locus_tag="PZ377_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359464.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
BASE COUNT         1102 a          645 c          515 g         1286 t
ORIGIN      
        1 acagcaaatc tcaaatatct ggaacctaat aaatcccctc actactgcta atagtgaagg
       61 gatttatttt attacttaga cgtaattata aatatatttg ttctattgta gtcatggtta
      121 atcatattta caaactatca tttctaaatc gcgcattatg aagtcaattt tcataaaccg
      181 tcattatatt tctggattca ttattcagct tctctaattt cgtctgcaat atctttatag
      241 tcaatatttg ctttagtgaa atctttatct tgaattggaa aatgctgttc aaatgtttct
      301 atattttgga tttctacttt aacatcttct acttcaaaat ctaagacatg tccaccaaag
      361 ttacgatcat cattcgcaaa gtgtacatga aatcctgctg atccgatacc atggaataat
      421 tctggtgtaa agaaaccaac gactgaacct ttgacatacg tttcagtttg ttcaggttgt
      481 cttctagctg aatcgattaa acgtgtataa ggtggttctt gagccggcat catacgtaca
      541 tgcatttttt taaataagcc tgaaattttt actgctgaaa ataaattttc actcaacatc
      601 ttttccttaa tttctgcaaa aactgcttct gaagatttat ctttcgtttc atagcttgta
      661 tctgctacaa atttagttac agttgcatat ggtgttaact cgtcaccttt taattctaca
      721 aattctttat gttcatttgc atggtaagct tttccatcta aaaagattac ctcaccgttt
      781 gaacctgtta gtgtagcgat acctaagtca ccatgttgca ataattcatt tattgatgca
      841 gttcctttta ataatcctgc cattaatgtg cctaatgtac catgttggta taaaacatta
      901 gtcataaaat ctcccactcc tctttaacat ttatctttat tttagttaaa tacgtcaggt
      961 aacatatttg ttgaaagttt gatattatct ttgtaattta caggtatatc aattaatact
     1021 ggaccatctg tttcatagcc ctctttaatt gccgcttcta attcttcttg attagtaact
     1081 cgtaaacctt tcgcgccaaa tgattctgca tattttacaa aatctacagg accgaagtct
     1141 acacctgacg aacgtttata tttcatttct tcttggaatt caaccatgtt atattttcca
     1201 tcattccaaa taagctggat gatatttaaa tttttacgta cggccgtttc taaatcttgt
     1261 gatgaaaata aaaagccacc atcgccagca acggacacaa cttgcgtatt agggcgcaca
     1321 agtgcagctg aaattgccca cggtaatgct acaccaagcg tttgcatacc attgctaaat
     1381 aataaatgtc ttggattgta acttctgaaa ttacgtgcca tccaaatata gtgacttcca
     1441 acatcaactg ttacagtagt atcatcagtt aatacctttt gcatagattc aataatttcc
     1501 actggatgta gaattccttc ttcatgcgtc gctttaatac cagtttcttc atcaatatgt
     1561 gttcttaatt gttctaattc gtctaaatgt tgttgattta taaatggctc atccactttt
     1621 tcagaaatca tttcaatcgt tttagcaata ttaccaatca actctttttt cggttgcata
     1681 taattagtaa tttcaacttg aacttcgtca atattgataa tttgtgtttc taattcttta
     1741 ttccagttac tagcttcgta ttcaattgga tcataaccga ttgtaacaac taaatcactt
     1801 ttacgtaata attcatcacc aacttgattg cggaataagc ccacacgacc gaagaaatga
     1861 ttttctaatt cacgactaat tacacctgca ccttggaatg tttctacaac tggtaaattc
     1921 gtgcgctcaa ctaatttgcg aatggcattt gtttcttctg cacttgaact tctcatacca
     1981 gctaataaaa caggaaatga tgcattttta atcgcttcaa tgacatcatt aatatcttgt
     2041 tcactcggta ctcctaaatt tggtttttgg caaagtgata tagctttaga ttcaactggt
     2101 gaagaaataa cgtcttgcgg aatactaata aaacttgcgc cattttttcc tgaagtagca
     2161 attcgaattg catttgtcat aacttctgat aatgattcag gatcttgtac ttcttcacta
     2221 tattttgatg aatattttaa tagcgcagca ttatcaatac tttgatgcgt taatcgtaat
     2281 aaatcattac gtttcacttg gccacctaac gctaatacag gatccccttc agatgttgcg
     2341 gttaataatc cagtagttaa attacttaca ccaggaccac ttgtaacaag tactacaccc
     2401 ggtttgcctg ttaatcttcc aataccttgc gccatcattg cggcgttttg ttcatgacga
     2461 gtgacaataa gttcaggacc atcatcaatt aaagcattaa atagatagtc aatctttgca
     2521 cccggaatac caaaaacata ttctacccca ttatttttca aagtatcaat aaccatatcg
     2581 gctgcagtgt actttttatc agtcatttat attcatttcc cttcaaatgt gatgtataga
     2641 tgtgtattac atgtaattac tttgtgattt gtaatctaat gatagttacg caaaattaat
     2701 gatgtttatc aaaattttac atgtacgatt agatttgtaa tacataaaaa ttgccttcag
     2761 tacattatat aacctttgtt agatgaataa aaatcgttat ttgtaatact aagtattcca
     2821 aaaatgcatg aatagatttt gtgcgttgtg attatgaggg atgttggatt gattgatgta
     2881 gaggattgtt acgatatttt tgggatgtgg tgtttagtca atggcgggct gaaattgtcg
     2941 ttttatggtg ggctgaaaat gctgttttat gtcgggcttg aatttgtcgt tttatggaga
     3001 tataaaaagc cacagcaaga cttagatcta actgtgactt atacatttca tacttatatt
     3061 tatcgattgg ttttatagtt ataaatgata gaagcagttc ctaaacaaat taagaagata
     3121 ctcattaaat atatagaaaa gttatctgtt ttaatataaa acctaaaaag tagaaacacc
     3181 agcatcgcaa caataaaata aacaggaata aaagctttct tccaaaatat agaatctttc
     3241 aattaagtca ctccttattt tgataacact cttaaatcat ggttcttgag ttatcatctg
     3301 gttatcatcc gatagctctt tcccttgaat atctattatt agaacactat cgtacggtct
     3361 ccgcaagcta aatgacaatt taataatgta cacttttgat tgtgtaagca tgtacaaatt
     3421 atacgtgtaa atcaataatt catagttgaa ttaattctat acctgagaca ttaattcatt
     3481 tatactctat tccatattcc ccacacaaac gcaaaaaaac acctcgacat aacatcgagg
     3541 tgttttgt
//
LOCUS       DANRDI010000209         3515 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.211, whole genome shotgun sequence.
ACCESSION   DANRDI010000209 DANRDI010000000
VERSION     DANRDI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.211"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..713)
                     /locus_tag="PZ377_002610"
     CDS             complement(<1..713)
                     /locus_tag="PZ377_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HDM6359465.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIV"
     gene            complement(697..1290)
                     /locus_tag="PZ377_002611"
     CDS             complement(697..1290)
                     /locus_tag="PZ377_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDM6359466.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            1641..2309
                     /gene="queC"
                     /locus_tag="PZ377_002612"
     CDS             1641..2309
                     /gene="queC"
                     /locus_tag="PZ377_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDM6359467.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            2311..2730
                     /gene="queD"
                     /locus_tag="PZ377_002613"
     CDS             2311..2730
                     /gene="queD"
                     /locus_tag="PZ377_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDM6359468.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            2734..3447
                     /gene="queE"
                     /locus_tag="PZ377_002614"
     CDS             2734..3447
                     /gene="queE"
                     /locus_tag="PZ377_002614"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDM6359469.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
BASE COUNT         1199 a          594 c          572 g         1150 t
ORIGIN      
        1 acaatcatga cattttctgc acgatctttc gaagaagttt gcaatgtttt ataatactgt
       61 tgatcttcag cttccgtttt acctctaggc attgtccctt tcatcggttt gcttatgata
      121 acgttatcga cattgttaaa ttgtcctttt tgaaaaaata attctggtga gatagacgct
      181 acttggattt catcagtttg taataacgca gtataattac cattactaaa ttgagttaat
      241 cgttcatata aagtactaat aggataataa atgttatccg ttaagcgcgc cgtatagttc
      301 acttgatacg tttcgccttc aacaattgct tgttggacac gtttaatatt agtcatcata
      361 acctcagaag attcaacaaa tgaaaaatga tactttgata cataagaagt ttgatgttca
      421 tatgttgaat ttatgctttc cgctttttca aaactataag ctgctgcata aatatcatct
      481 ttagctaatg aatgtgtaca catagcatga ttaaaatact ttgccgcttc gtaacttaaa
      541 tataacgaga catatctacc ttgtcgttgc tgcgcttgtg caaagtgtat cacttctccc
      601 acatcagcca acttagtagc aacatacttc tttataaatc cctttaattg aatatggtat
      661 tgcttatatt cattttcagt taaatagtag cgataattat attctattct cacagtaatc
      721 acctaccttc gataaaaata attcaacttg tcgataaccg tattcactca aaatagattc
      781 aggatgatat tgcacaccaa aaaccggaaa tctaatatgc tcaaatgcca taataatcgc
      841 ttcatcgttt tttgctgtaa tctttaagca atttggaaaa gtcgctccgt cagcaattaa
      901 tgaatgataa cgcattacat tgaaattttg aggcagtcct tgaaaaatac cttcattggt
      961 atggcgtaac tgtgtagtat gtccgtgtac aggatgatag ccgtgaatga tatttccacc
     1021 aaaataagac acgatacatt gaaatcctaa acatacacct agtataggta cacgctgata
     1081 aaattgttct aacacttcat tcaagatagg ataatcatcc ggattacccg gcccaggcga
     1141 aataacaatt gcttttggct tcatattaat gacgtcttct atcagcagat tatcaatacc
     1201 aacaacttga actgttagtt tcgtttgagt cttaatatag tctattaaat tatatgtaaa
     1261 tgaatcatta ttatctatga ctagaatcat tgtatactcc gttctaaatg tgttttattt
     1321 ttataatatg tattggatgt agctaaaact ttaaaagcat tgtcattatc ctgaccttga
     1381 ttttaactaa tatatggtat attctattca tcgtacataa atgaatatca gaggttccta
     1441 gctgaaaccc tctataaaaa actagacatt gaaatttcaa acattcgttg gtaaaccttt
     1501 aatatatatt ataggagcaa cccatttctt tcgtctatct tttatggaga taggcttttt
     1561 ttattttata aattaaaaac gggcttatca atgccattaa cctctttatt aatattatta
     1621 atgataggag caattacaaa atggaaagtg tattaaataa tgaaaaagcc atcgttgtct
     1681 ttagtggtgg tcaagacagt acaacatgtc tcttttatgc aaaaaaacat ttcaaagaag
     1741 ttgaactcgt aacgtttaat tatggccaaa gacatgatac tgaaattgaa gtcgcaaaac
     1801 aaattgcaca agatcaagga atgaaacatc acgttttaga tatgtcatta ttatcacaac
     1861 ttactccaaa cgcattaaca caacatgata tggaaattac taataatgaa gatggtatac
     1921 ctaatacatt tgttccagct agaaatttac ttttcttgtc gtttgcaggc gctctagctt
     1981 atcaaattgg ggctaagcat attattacag gcgtatgtga aacagacttt tcaggctacc
     2041 cagactgtcg cgatagtttt ataaaatcaa tgaacgtaac attaagccta gctatggaca
     2101 aagattttgt cattcatact cctttaatgt ggttaaacaa agcagaaacg tggaaattaa
     2161 gtgatgaact cgaagtttta gattatattc gtacaaaaac attaacatgc tataacggta
     2221 tcattgggga tggctgtggt gaatgtccag cttgtcattt acgtcaacgt ggactaaatc
     2281 aatatcttga aagtaaagga gcgctttaaa atgttacaac aaatctatcc tagtacaacg
     2341 catccatatc aattcgaatt aaataaagat tttaattttt cggctgcaca tcacattcca
     2401 tgtgaagaag caggtatttg tcaaaatgtc catggtcata cttactttgt taatttaaca
     2461 attgtcggtg ataaactaga tgacactggc ttcttagtga actttagcca tttgaaaaag
     2521 atgatacacg gtaaatttga ccatcaactg ttaaataact tacctgcttt taaaaacaaa
     2581 atcccttcaa ctgaaatcgt agcggaaaca atttatcaaa ttgttaaaga aaatttggca
     2641 tcgctcgaac accaaccaaa atgtattcaa gtatttgtaa gagaaacacc aacaagttat
     2701 gtcgtattta gaccaaagga acaggtgtaa ctaatggcta aaattcctgt actcgaaata
     2761 ttcggtccaa ccattcaggg tgaaggtcga gtaatcggta gaaaaacgat gtttgtgaga
     2821 acagctggat gtgattatcg ctgtagctgg tgtgattctg catttacttg ggatggtagt
     2881 gctaaaggcg atataaaact catgaccgct gaagaaattt atgatgaatt aaaacgaatt
     2941 ggtggcgatt tatttaacca cgttacaatt tctggtggta acccagcatt aattaaaggt
     3001 atccaagagt tagttgactt atttcaagat aaaggtattt tcagcgcact ggagacacaa
     3061 ggcagtaaat tccaaccttg gatgacacaa attgatgatt taacgattag tccaaaacca
     3121 ccaagttcaa ctatgacacc tgatttaaaa aagttagatg aagtgattac acaatgcgta
     3181 ccttcatcat taaacttaaa agtagttgta ttcgacgata aagattatga ttttgccaaa
     3241 atgatacacc accgttatcc agatattccc ttctatttac aagttggtaa cccatattta
     3301 tcagacagcg tagataatca taccgaaaag ttgttagaac gctacgagca gttggtggat
     3361 ttagttatgc aaagtaatga tatgaatcac gtttatgtgt taccacaact tcatacatta
     3421 ctttggagca ataaaaaagg tgtataaagg ataacttatt ttactttttg caagtcatat
     3481 ctaatgaata acaatatatt aattgatata atagt
//
LOCUS       DANRDI010000210         3510 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.212, whole genome shotgun sequence.
ACCESSION   DANRDI010000210 DANRDI010000000
VERSION     DANRDI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.212"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(102..347)
                     /locus_tag="PZ377_002615"
     CDS             complement(102..347)
                     /locus_tag="PZ377_002615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359470.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            complement(313..948)
                     /locus_tag="PZ377_002616"
     CDS             complement(313..948)
                     /locus_tag="PZ377_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDM6359471.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            complement(985..1627)
                     /locus_tag="PZ377_002617"
                     /pseudo
     CDS             complement(985..1627)
                     /locus_tag="PZ377_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
     gene            complement(1751..2380)
                     /locus_tag="PZ377_002618"
     CDS             complement(1751..2380)
                     /locus_tag="PZ377_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDM6359472.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            complement(2833..3462)
                     /locus_tag="PZ377_002619"
                     /pseudo
     CDS             complement(2833..3462)
                     /locus_tag="PZ377_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
BASE COUNT         1305 a          567 c          369 g         1269 t
ORIGIN      
        1 cattaagtat tcatcattat atttaataat tctatatttt gggtttttat tgatgatttt
       61 agattcgcat agcaacattt accagctcca tttcttaggg cttatatgat tagaaatact
      121 ttttgaagct tcaccatgac attttcaaca ccatctactg ttttttctgc agatttttct
      181 acagcatcta taccaacgtc tagtttatgt cctatccaat cgtttttatc ttttattcca
      241 aattcatttt tttgattggt ccaatataca agtattgccg aaatagaacc tgcagtaaat
      301 ccagctaaac catcattttt ccaccgtgtt agttttagat aatacataaa tttttttgtc
      361 aataatcgat gacatgttta acagaaagaa acctgcagtc attaccaacc aaccaataaa
      421 gcctattata ttttgaggat ttaaagttaa taacatactt agagccatta ttgaaagacc
      481 gccaaataaa aaataataaa atattgtgaa acaaaagttt tttaatgaag gaaataaaat
      541 tattttccct ttactagagt tattattata aatttccaac cttagcttac gattcagaaa
      601 taaaaaaagt attaaaacac ccagaaatat tgcacaacat attacaatca ccatgttttt
      661 ctctaattga atattaagtg aaggaatata ttttctaaac gtaactccga acaaaatagt
      721 actacctgca actggccaaa aagctttatt tttaacaggt ttgacaatgt ttaaatcgtc
      781 aaattctttt ttactgattt tgacatattt ttttggaatt aaccaattaa tcataggaag
      841 aaataaagtt aaccaggtac ttactaaatc aatcattaaa tattcatcac catatttaat
      901 aactctatac ttaggatttt tgttgataac tttagattca catagcaata tttatcacta
      961 ccattccttt gtttattatt cctattaaaa cttttcaatt ccatttgatt taaataatac
     1021 atgaacttta ttgtcatata gtgagcacat atttaaaaat aagaaaaaca aaaatattac
     1081 aaacaaagtt ataaataata ttatattttg aacttcaatc gtcaataaca tataaatagt
     1141 aaagaatgaa ccaccaccta aataaatata taaaactatt gataaacaaa gactctttaa
     1201 agtgggaaat atgattatct tattgctttt atttttgttt tcattataga tttgtaactt
     1261 taacttttga tttaaatata aaaaaaaaca gaaaaatacc tagtattatg atgcaacaaa
     1321 ttaaaattac tatactatat tcatatttaa cgataaataa atgggaatac cttcttaaca
     1381 taacaccaaa taagaccgta cttcctgcca ctggccaaaa agaattgatt ttagctgtct
     1441 tcacaatgtt taaagtttca aaatcttttt cgctgatttt gacgtacttt tttggaatca
     1501 gccaattaat cattgggaaa aagagtgcta accatgtact tactaaatca atcatcaaat
     1561 attcatcttt gtatttgata attctatatt tgggattctt attaatattt tcagtttcac
     1621 aaagcaatgt ctccacttct tttcaattgg ttttatattg atacactttc atatacatta
     1681 gaagtcattt aaattctact agcgtcttaa aaggattgtg tcgacaacat ttagaatatt
     1741 ttaaatgtaa tcaattattt ttcattataa cgcgaacttt ttcattacct atcgaagtca
     1801 tatttaacaa aaagaaaagc attgtcatca taatccaata aataaatatt attatatttt
     1861 gaggttcgat tgtcataagg gcaattaatg aaaatattga aaaacttcca gcgaaaatat
     1921 atccgaaaac taaaaaacag ccaagtttaa acgttggtat taatataact ctcttatttt
     1981 tttcaatatt tgtatcaaac acttttaact taagctttct atttaagtta aaataaaaaa
     2041 ctataacaca aattaaagcg agagcacaca atatcattac aactagctta tctaattgaa
     2101 catcaaaaac atgtatgtat ttccttaaag taactccaaa caaaaccgaa cttcctataa
     2161 ctgaccagaa aacattattt ttatttggtt taacaacatt taaattttca aattcttctt
     2221 cgctaattat cgcgtacttt tttggaatga accaattaat gaaaggaaaa aagtacacga
     2281 accaagtgct tactaaatcg actaataagt attcaccgtt atgttgaatg actttgtatt
     2341 tcggattttt ataaatgact ctgacatcgc aaagcaacat ttaccactcc cgtttcatta
     2401 gttttaaatg atccccaata ctttttgtac cttcaccaac tatcgttcca acactatcta
     2461 cagctttttc tgtggtattt ttaaacagcg tctatttaat gtcttatcca attagtttta
     2521 tctcttaatc caaatttatt agtatgaaaa gtccaatttt taagaattgt ctaaacagat
     2581 tccgcaacaa aaccagctaa aaccgtccat actattggat taaaaacgcc aatgttataa
     2641 gagctatcat acaaaataac acgctctata tttcattttt aaattaaaac agttttttcc
     2701 tttatcaatc acaatgggca ttttcacttg gtatattcaa acattactat ctattttcct
     2761 gatttaagat atgattatat ttttttgaac caccgtttaa atatgcgata ctaccaattt
     2821 cgatcatttt tactactcgt ttattcttaa aataacatga acatttttat tacctatcga
     2881 agccatattc actaaaaaga aaaacatcgt gataataacc caagttaaaa atattattat
     2941 attttgtgac tctgtcgtca aaagaatggt taaagtaaat attgaagcgc ttcctaaaaa
     3001 aaagtacgca aaaactatta acaatccttg tttgaaagtc ggtattaata caactctatt
     3061 cttattaacc acgttggtat taaaaatttt taatgttaat tttttattta gataaaaata
     3121 aaaaattaac atccctatta acatgataaa aaatacagtg attgctagtt gtttttcaaa
     3181 ctgaacatta aagaagttac cgtactttct caatataatt ccaaatagaa ctgaacttcc
     3241 tgcaaccggc caaaaaacat tatttttaac aggctcgact atatttaacc tttaaaattc
     3301 attttcgcta atttttgcgt attttttggg caaaaaccaa ttaatcatag ggaaaaaata
     3361 agtaatccaa gtacttacta aatcgaccat gaaatattca ttattatatc taataactcg
     3421 atatttagga tttttataaa tttgtctaga ttcacaaagc actgtttacc acccctaatt
     3481 aacaattgaa aactaactaa atttgtaact
//
LOCUS       DANRDI010000211         3479 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.213, whole genome shotgun sequence.
ACCESSION   DANRDI010000211 DANRDI010000000
VERSION     DANRDI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.213"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..99
                     /locus_tag="PZ377_002620"
     CDS             <1..99
                     /locus_tag="PZ377_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359473.1"
                     /translation="ENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            complement(184..867)
                     /locus_tag="PZ377_002621"
     CDS             complement(184..867)
                     /locus_tag="PZ377_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDM6359474.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(1233..1931)
                     /locus_tag="PZ377_002622"
     CDS             complement(1233..1931)
                     /locus_tag="PZ377_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HDM6359475.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(2311..3009)
                     /locus_tag="PZ377_002623"
     CDS             complement(2311..3009)
                     /locus_tag="PZ377_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HDM6359476.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(3353..>3479)
                     /locus_tag="PZ377_002624"
     CDS             complement(3353..>3479)
                     /locus_tag="PZ377_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDM6359477.1"
                     /translation="INLKDGEKQEIDLGDKLQFERMGDVLNSKDINKIEVTLKQI"
BASE COUNT         1065 a          602 c          398 g         1414 t
ORIGIN      
        1 gagaattttg atgttggtgg tggaaatttt acctttatgg agattgggat aggattcatg
       61 acacacaaac caaaacggct taacgacgat atcgattgat gcatcctttt tgtataagct
      121 atgcgcacca atttacattt caccgttaag ccgcttcaat tacttttaat tattcgtaat
      181 gatttacttt aagttaactt caatatcttt aatttgtttg ctttctatga cttcccccat
      241 atatttaaat tttaatttag atcttaaatc aaggttataa aaactgccat ctttcaagct
      301 aattttgacc ctaccatctt ttgagattgt tttataaagt ccgtattttt caattagatg
      361 ctttctcaac ttataatcca gttcttttag tgttattttt tcttttttaa tgtaataagg
      421 gtaacctttc acaaaagcat cttcaccctt ttcttttgaa atatttaata ttggtgcact
      481 gacaactcca gatgttttat tattctgtat tacaccacca acagtataaa atatatcgtg
      541 cttatctctt ttttcttgaa caaagaaaac atctaacccc tcataactac gctgttgaaa
      601 tttactttta tcatttccag gcagtaaaac ttgaatctta atacctctaa acttaaaacg
      661 taagttgttt ttaccatgtt tcaaagcact aatatttttc atttccatag tctttccagt
      721 gtagtatcgg tataatgctt ccttgtcatg tttatttact gacttttgat tttgttttgc
      781 atgacctgaa tgaacttctg ttgttaaagt tcctgttgtt aaaattccta aagctaatgt
      841 cgcttttgct aatgctgtaa atttcatatt tatttgctcc aatcttaata tattggattg
      901 ttttcattac gtaatttgaa tcatacattt ttattataac gtgcatcgtt tcatacgata
      961 attaacctcg atttaaatat acttgaatta ttcttaatga aaaacaaata tattttaatg
     1021 ttcaggttaa atgactttca ttatttcatt tagttgtttt accggattat cgtttactac
     1081 aactgatgat gttactctgt tctgctcttt gaattacaaa aacaacttaa caacagtaat
     1141 gtatttaaca cagttcctta tataaggaca caaatgccaa ttcacatttt gtcgttaagc
     1201 cgcttcttaa gctattatgc tattaactcg aactaattca aattcacttc aatattttta
     1261 atttgcttac tatccattac atcaaacata cgttcaaaac ttaatttttc acttaaatca
     1321 atttcatgct tcttttcgtc tttcatattg ataacaattc tacctttatc agacgttcct
     1381 ttatacaatc tatatttttc gattaagagt tttcttattt taaagtccag ttccttcaat
     1441 gatacttctt ccttttgaat aaaaaacaac tcgtttatcg aaaagccatt tttagcatca
     1501 acttttttaa cttgtagact tggattactt acaaatccaa acactgatct cacatttttc
     1561 tttgttatac cgccaacgct atatatgcca ccttttatat ctattaattc aggtactgca
     1621 aagacatcaa ggccatgtgt tttttcttta tatttatttt tatcgtcacc aagtaaaaat
     1681 actttaaaag ctttatttcg ttgtttaaag tttaaaacgt tagagccata gtaatcttcg
     1741 cttttaacac taatgtttgt tggttcaaaa ctttcttcag aatagtattg atgtagcata
     1801 ttgatataat atttgcgttg tgtctcatca agttctactt tcgcgtgacc agtttgactt
     1861 tctgctgtaa acacacctgt agttaatatt cctaatgcta atgttgcttt agctatcgtt
     1921 gttaatttca tatttttttg ctccaatctt aatgtattgg attgttatta ttacgtaatt
     1981 tgaactatct gtctttatta taaggtgcat caaaacagac tgacattaac gctcatttaa
     2041 ataaacttga atcattatta atgaaaatca aacgtatttt aacattcgta ttaactgatt
     2101 tttaatagca acataaatat ttttttggtg aatcattgtt ctttccgatt agttttaaat
     2161 cttaagctat tggttataaa gtgtacaaaa aagcagctta ataacgtagt caatgttcga
     2221 tatgttttta ttataaaaac acgtatcaaa atcattaaac atgggattat taaaccgctt
     2281 cttaagcttt cattctatat atatcattga ttatttcaaa ttcacttcga tgtttttaat
     2341 ttgttcacta ttaatgacat ctgccatacg ctcgaaatct aatttatcac ttaaatcaat
     2401 ttcatactta ttttcatctt tcatattaat aacaattcta cctttatcag ctgacccttc
     2461 atacagtttg tattttttaa tcaacagttt tcttatttta aaatcaagtt ccttcaatga
     2521 cacttcttcc ttttgaataa agaaaaattc atcaatagaa aaaccgtctt tatcgtctat
     2581 ttttttaact agtaagttcg gcgttcttag agactcaaat attgatttta cgtttttctt
     2641 tgttacacca ctaacactaa atattcttcc atctaaatct actaattctg gtaccgcaaa
     2701 gacatctaaa ccatgtgttt tttctttgta tttattttca tcttttccca ataaaaacac
     2761 ttggaacttt tgattttgtt ggttaaaaag caaaacgtta gagtcataat aaccttgact
     2821 ttggccacta acatttgtta actcataact aggtcctgaa tagtattgat gtaacttgtt
     2881 tgtatcatat aaacggttca ttttttcata ctttcctttt gcattaaccg attgattttc
     2941 tgttatcatt acacttgttg ttaatattcc taatataaat atcgctttag ctatcactgt
     3001 aaatttcata ttcatgtgct ccaatcttaa tatattggat tgcttttatt acgtaatttg
     3061 aataatacac ctttattata aagggcattg agacagactg acattaacac tcgtttaatt
     3121 aaacttaaat cattgttaat gaagagctaa ttaatattca atgggcatca ctttcaagag
     3181 caatgtaaat ttttagtatg ctcactcata acctgcgtta taaaacgtaa aaaaaagcct
     3241 aacgacaagg ttgttttaaa tacgctcctt aatgtaaggt gtacgtatct attcaacatt
     3301 tcatcgttaa gctgcttcaa atttattact ttagagtcat tgattactta ctttaaattt
     3361 gtttcaaagt cacttcaatc ttattaatat ccttactatt caacacatca cccatgcgct
     3421 cgaattgcaa tttatcacct aaatcaattt cttgcttttc tccatctttc aaattgata
//
LOCUS       DANRDI010000212         3419 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.214, whole genome shotgun sequence.
ACCESSION   DANRDI010000212 DANRDI010000000
VERSION     DANRDI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.214"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..182)
                     /locus_tag="PZ377_002625"
     CDS             complement(<1..182)
                     /locus_tag="PZ377_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDM6359478.1"
                     /translation="MKRASYSVETKYKAVEMKAAGFPTKEIMKELNIRNRTQVKNWWR
                     WYRNGESYRFSQHVGK"
     gene            complement(694..1191)
                     /locus_tag="PZ377_002626"
     CDS             complement(694..1191)
                     /locus_tag="PZ377_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6359479.1"
                     /translation="MKLRALEYSDLLFVHELNNEYSIMSYWFEEPYESLTELQHLFDK
                     HLLDESERRFIVEDENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAI
                     IYAFNILNMHKIYLYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLLK
                     SEYIL"
     gene            1689..2816
                     /locus_tag="PZ377_002627"
     CDS             1689..2816
                     /locus_tag="PZ377_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDM6359480.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
     gene            complement(3071..3295)
                     /locus_tag="PZ377_002628"
     CDS             complement(3071..3295)
                     /locus_tag="PZ377_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359481.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYEEFINDDSITTVLNNDLQTIINHYLSK"
BASE COUNT         1165 a          491 c          535 g         1228 t
ORIGIN      
        1 tgtttaccaa catgttgtga aaatctataa ctttccccat ttcgatacca tcgccaccaa
       61 tttttcactt gtgttctatt tctaatattt aattctttca taatttcttt tgttggaaat
      121 cctgctgctt tcatttcaac tgctttatac tttgtttcta ctgaataaga agctcttttc
      181 atagaaaaaa acacctccgt atgattcatt ttaatatgaa ttcaacgaaa gtgtttttat
      241 ataattccca ctattggggt cagtctaaaa ttaaaattga taactttttt aataaaatag
      301 atacactaat tttaaaacaa ggtaaaaaaa tagaattatt aaaacaacgc aaacaaggct
      361 tactacaaaa aatgtttgtg taattctgat aacgtcctat tatgttaatt gaaaatagac
      421 aaaaataaat tgagagcatc ccttaccgca aagtgaagga tgctctattt ttgttgaaat
      481 taatataagc ttcattctga tagatagtca ttaataattg tttgcaaatc gttattaagt
      541 accgttgtta cagcgtcatc attcataaaa tcctcgtaga ttttatcaag aatttattcc
      601 tcttacagac attcgcgaga agtccgtttt aattttatta gaagtaattc aggtttgaac
      661 ctacctaaat gaatatatga gttatctttt atactacaaa atatattcag atttcaataa
      721 tgacataaaa taggcatctt tatatttacc tttagtgtag aattgctctt taagtaatcc
      781 ttctgtttta aatccttgtg actcgtatat atgaatagct tttttgttat ctgcatcaac
      841 atatagatag atcttgtgca tatttaaaat attgaatgca taaattatcg ctttttcgaa
      901 tgcgaatttt gcataacctt taccactgaa ctcaggttta ataattattt gtatttcaca
      961 attacgatgg atgtaattaa tttctactaa ttcaacaata cctacgactt gattttcatc
     1021 ttcaacaata aaacgtcttt ccgattcatc taataaatgc ttatcaaata aatgttgaag
     1081 ttccgttaag gattcataag gctcttcaaa ccaataagac ataatagaat attcattatt
     1141 taattcatga acaaaaagta aatcactata ctctaatgct cttagtttca taatcccact
     1201 cccaaaattt tcttatatat ttgcattata aatataaata acgaataagt cattattcac
     1261 tatgaataaa tctattttaa caattcacta catactaatt ctcattttct tattattctc
     1321 taatatctct gatttattac tcagtgaaag atgccctatt tttatcagta tatagttttt
     1381 attttgatag atattgatta ataatcgtct gtaaatcatt gttaagtaag gttgtgatag
     1441 cgtcgttatt cataaaatct tcataaattt tatcaagtat ttctttaata tattttttaa
     1501 tcttcacacc ttaaattcac ttgaaaacca aatcacttct aaattatgat tttacataat
     1561 aaatttttaa aaattggtat tatataaaat aatctcaata atcagaattt tttgtaaata
     1621 ataatattaa agtgtaaaat aacagataag aatagactta atgtatataa attaaaggag
     1681 tgttggaagt gaaaactaga gcagcagttt tacatgaaat gggggctaaa aagccctata
     1741 aagaatctaa acctttaaaa attgaatact tagaacttga taatcctagc gaacacgaag
     1801 tattaattaa aattcatgca gctggattat gtcactctga tttgtcagta attaatggta
     1861 acagacctag acctttacct atggcacttg gtcatgaagc ttccggtgaa gtaattaaag
     1921 ttggaaaagc tgttacaaga gttagcgaag gagatcatgt ggtatgcaca tttattccca
     1981 gttgtggaaa atgtatccca tgtaaagaag gacgtcctgc attatgtgaa aacggagcaa
     2041 tatctaatga aaaaggcgaa atgttagagg gagggatgcg tttatctaat gatgaaggaa
     2101 aagtatatca tcacttgggg atatcaggtt ttgctgaata ttctgttgtc tctgaaaact
     2161 ctatagttaa aattgataaa aaaatacctt ttgaacgtgc agctgcattt ggttgtgcta
     2221 ttatcacagg tatcggtgct gtggtgaata cagcccaaat tcgttctggt agtaatgtag
     2281 cggttgttgg tttaggaggt attggattga atgctattat tggagctaaa ctagcgggag
     2341 ccaatgaaat tattgcttta gatattaacg aagataaatt tgaattagca aagcaatttg
     2401 gggctacagc aacatttaat tcaagcgata aagatatcga tgagcaaatt aaagaatata
     2461 ttcctggcgg agtagaatat gcttttgaaa cagcgggtgt agtgccagct atgaaagttg
     2521 cttatcagat tactaaacga ggggggacaa ctgtaacaac aggactgcct aatcctaaag
     2581 ataatttttc tttccctcaa gttactttag cggctgaaga acgtaccatt aaaggatcat
     2641 atgtaggaag ttgtgtacct gacagagata taccaaggtt cgttaatcta tacaaccaag
     2701 gacgtttaaa tattgattca ctcatcagtg aggttatcac tttagatgaa attaatgagg
     2761 gatttgatcg tttgtctaat ggtgaagtag gtagaattat aatgaagatg cattgaatat
     2821 aatagaattc aagtcgttcc tcctcttgat ttctatgaaa agaataacaa tttgagtagg
     2881 aagattactg acaaatatta catttgcatt gtgactgcca atctaattgt atttttacat
     2941 ttcatatcta tatattagaa gataaatgtt ttaaaactaa ttactaatag tttttttaga
     3001 taaaaaaaga tataggagca tccttcaccg caaaagtgaa ggatgctcta gttttattag
     3061 aatatagttt tcattttgat agataatgat taataattgt ttgtaaatca ttgttaagta
     3121 ccgttgtaat agagtcatca tttataaact cttcatagat tttatcaagt atttcctcgt
     3181 catcgatggc tgtgaaatga tgcaccagac cttttactat tgaaatgtaa ttttgtgatg
     3241 ctaaacaact agcaaccgtt cgccaattgg attgtatagt gttgtcatgg ttcatagata
     3301 atactccttt actttagtgt ccatttttga cgtgctttag ggttgagtgg atgcataact
     3361 tcgttcgtta ctggatttat gagctttttt actttctttt tatgaggttt taacatttc
//
LOCUS       DANRDI010000213         3403 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.215, whole genome shotgun sequence.
ACCESSION   DANRDI010000213 DANRDI010000000
VERSION     DANRDI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.215"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(73..258)
                     /locus_tag="PZ377_002629"
     CDS             complement(73..258)
                     /locus_tag="PZ377_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359482.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
     gene            complement(347..1411)
                     /locus_tag="PZ377_002630"
     CDS             complement(347..1411)
                     /locus_tag="PZ377_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDM6359483.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1559..3001)
                     /gene="lmrS"
                     /locus_tag="PZ377_002631"
     CDS             complement(1559..3001)
                     /gene="lmrS"
                     /locus_tag="PZ377_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HDM6359484.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3323..>3403)
                     /locus_tag="PZ377_002632"
     CDS             complement(3323..>3403)
                     /locus_tag="PZ377_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance protein SepA"
                     /protein_id="HDM6359485.1"
                     /translation="IMVIIGFLYVCKLQFYPESKNLNNRK"
BASE COUNT         1143 a          669 c          443 g         1148 t
ORIGIN      
        1 gttcaatctc ttttattcta cttcatttat ttcttgaagt caataacttt ttgaacgatt
       61 actttattta ttctatattg tttttatagt tatttcaatg gtaagtttta cacttttgaa
      121 attcttcttt aaaaacaact gcgtcgtttt tgacgacttt atcatattat caactttgga
      181 atttaaagtc aataactttt ttaaaaactt tttgtgttca caacccgctt ctttttcaac
      241 gcgtttattg cttaacacaa gaacttattt taccagcatt ccaaaacaaa tcaacataaa
      301 aacgtacaaa ataaaagtaa ttttgtacgt ttagcatata ttatacctat ttatttgtag
      361 cagctataac tttttgtgca atcgagctat aaattttacc tagacgatca tctgattgat
      421 atattgacgg tgcaaaatct tttggattcc aagatggttg ctctaaaggt aattccccaa
      481 gtaattgagt attaagttca tcagctaact tagtaccgcc acctttgcca aagacatatt
      541 ctttattacc cgtctcttta ctttcaaaat aactcatgtt ttcaattacg ccaagaatag
      601 aatgatccgt atgttttgcc atcgcacctg cgcgagctgc aacaaatgct gctgtaggat
      661 gaggtgtcgt tacaataatt tccttacttg aaggtaacat cgtatgaaca tctaaagcta
      721 catctcctgt tccaggtgga agatcgagta ttaaatattc aatgtctccc catttaactt
      781 ctgtaaagaa attcgtcaac attttaccta acattggccc tctccatata actggcgcat
      841 tttcttccac aaaaaaggcc attgatataa ctttaacgcc atgacgttca actggaatta
      901 cttccttccc tttaattcca ggcttttcat caatacccat catatctggt acactaaatc
      961 catatatatc ggcatctact aatccgactt ttttcccttc acgagctaag gcaacggcta
     1021 aatttactgc aacagtagat ttaccgacac cacctttacc ggaggctatt gcaataaatt
     1081 cgactggatt attttgagat aataggcctt ctatcgtttt aggtttattt tcttcttttg
     1141 gtttaaattg atttactttt tcttccggca atgtttcaaa tcgtataccg accgttttcg
     1201 caccgttttc ttttaatgca ttaacaacag ccatctgtaa atctaattgc ggtgcaccac
     1261 ctaattgtgc cattgctaat ttaacactca catgttcttt ttcttcctta atagaaactt
     1321 caacaatacc ttctgtttct tttaaaggca catctataat aggatcttta atttctccta
     1381 ccaattcttt cacttgatct accgttaaca aagcaaatcc cccttaatca ttttaagaca
     1441 tctacatcat agcaaataac aatttttgct tagtctatca ctaaaagtat aaaacttttt
     1501 attaaataaa aaaagcagtt agcctattat tttaaataga ccaactacta taattatttt
     1561 aaaatttcct tctattactt tctatttctt taggttctct tataaaaagt gctatcacat
     1621 aagctgccat aacaagcaca gctacaaaag caaatgagat atccacccct atagcaatgg
     1681 attgctgttt aatttcagtt gcagttaaat gcgatggcga tgtagataaa aatatttttg
     1741 caccaaaact cattaatgta accattaaag ctgtgcctag tgaaccagcc attacacgac
     1801 caaagttcat aatagccgtg ccatgtgaga tttcttcatt tctcaaagaa ttaattcctg
     1861 tagtatttat cggcatcatg agtaaagaaa ctgaaaacat tctaattgca tatacgacta
     1921 ttaaatacgt ataagatgtg tctgtatgca agaaacacaa cataattgta gtaattgtta
     1981 aaattgtaaa accagtataa atcaatggtc taggaccata cttatcataa aatttacctg
     2041 taaaaactga cataatacca tttattattg caccaggcat gataacaagt cctgataaca
     2101 aggcagataa agataaactg ttttgaacat atagcggtat aagcagcgca ggtccaacca
     2161 ttgacatcat tataatcatt gaactaatcg tacataatgc aaatgttcta tttttaaaaa
     2221 cccttaaatt taaaagtgca ttagtaatat taaattgtct acgtataaat aaatagataa
     2281 taaccatact taatatcaac gcacctatta ctattggact tgtaaatcct aaatctcctg
     2341 cactactaaa tgcgtatagc attaacccga acccaatagt tgaatacatt actgaacgct
     2401 tatctaattt cgtatatttc acttcattgt aactcgagat tgaaaccaaa ccgaaaacaa
     2461 aggcaagtat agcaattcct aaaattataa taaatggaac tcgccaactc gcttgatcaa
     2521 taattaatcc tgtagctgta ggtcctattg ctggtgcaaa ttgaattact aaacctgcta
     2581 gtcccattgc aaaacctcga tgttctttac tgaacaatgt aaataacgta aattgcatta
     2641 agggcataag tacgcctgca cccatcgctt gaattacacg agctaacatt aaaactccaa
     2701 aattcggagc taaggctgca acaatagaac ctaataaaaa tgtccccatc gccgctaagt
     2761 ataaaggtct agttttaatt ctatccatta gatatgccgt cagaggtatc atgacgccat
     2821 taacaagcat aaacccagta actagccatt gcgatgtact ttcattgata tttaattctc
     2881 tcattatact aggtaacgct gtatttaata atgtttgatt taaaatagct acaaacgcac
     2941 taatcaacat aacagcaact atgaagtttc gtctccgggt tgttaattca actttagcca
     3001 tcataattcc tcctttttaa aacagatata cacttgtccc tttaatacgt atagccatga
     3061 acataatggg agtagtactc aaataataaa gaagcactac ataataaatc attattggtt
     3121 cttacacatt aggcacagct aatgtgtact taaaaactaa caatataaca ttgtagggcc
     3181 taataattta ttttatgtct cagactcata caatggttat ttaacttatt taccacaaat
     3241 tgagcactaa gactcactat tatcgattct tctattgaac aaaaagaagt tggaacacat
     3301 atgtgccctc aacttcagat atctattttc tattatttaa atttttactt tcagggtaaa
     3361 attgaagctt acaaacatat aagaacccta taataaccat gat
//
LOCUS       DANRDI010000214         3371 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.216, whole genome shotgun sequence.
ACCESSION   DANRDI010000214 DANRDI010000000
VERSION     DANRDI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.216"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..776
                     /locus_tag="PZ377_002633"
     CDS             <1..776
                     /locus_tag="PZ377_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDM6359486.1"
                     /translation="TLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTLKLYPTKN
                     LDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNTRTTKGYY
                     FISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFVQYGNFKD
                     INDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLKGDGDLKG
                     SSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(780..902)
                     /locus_tag="PZ377_002634"
     CDS             complement(780..902)
                     /locus_tag="PZ377_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359487.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            1337..1528
                     /locus_tag="PZ377_002635"
     CDS             1337..1528
                     /locus_tag="PZ377_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359488.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(1667..1852)
                     /locus_tag="PZ377_002636"
     CDS             complement(1667..1852)
                     /locus_tag="PZ377_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359489.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(1788..2069)
                     /locus_tag="PZ377_002637"
     CDS             complement(1788..2069)
                     /locus_tag="PZ377_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDM6359490.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            2358..3053
                     /locus_tag="PZ377_002638"
     CDS             2358..3053
                     /locus_tag="PZ377_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDM6359491.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT         1239 a          479 c          597 g         1056 t
ORIGIN      
        1 taacgctcgg tatatgcttg gtattactca ttatattgat tgtaggttat gtcattatga
       61 caaaaacaaa tggtcgaaac gcccaaatta aagacacatt taatcaaaca ttaaaattat
      121 atccaaccaa aaatctcgat gatttttacg ataaagaagg ctttcgagat caagaattta
      181 aaaagggtga taaaggtact tggatagtta attctgaaat ggtaatcgag ccaaaaggta
      241 aggatatgga aacgagagga atggtgctct atatcaatcg caatactaga accacaaaag
      301 ggtattattt tataagtgaa atgacagatg acagtaacgg cagaccaaag gatgatgaaa
      361 aaaggtatcc ggtaaaaatg gaacataata aaatcatacc aacgaagcca ctaccgaatg
      421 acaagttaaa aaaagagatt gaaaacttta agttctttgt acaatatggc aactttaaag
      481 atattaatga ttataaagat ggtgatattt catataatcc taatgtacca agttattcgg
      541 caaaatatca attgaataat gatgattata atgtccaaca gttaagaaaa agatatgata
      601 ttccaaccaa acaagcgccg aaactattat tgaaaggcga tggagattta aaaggttcat
      661 ccgtaggttc tagaagtctt gaatttacct ttgtcgaaaa taaagaagaa aacatttatt
      721 ttacagattc tgtacaatat actccaagtg aggatacaag gtatgagtca aactgaatat
      781 caaataaaac ctggtaatat aacaagtaac tctgaagaaa caagttcgat atctaaagtg
      841 agctgtgaaa tataggtagc catttcaaaa aatttaaagg tgaatttgat aatgtagctc
      901 aaggagattg ggttaaaaag gcgaagaatg aactggatga tattagtaag aaattaaaaa
      961 atattcaaag aacggaagtt taatagctta tatgattctt ggagctaaga cagcatgcgt
     1021 tcattcatgc cattattaat ataagcaccg caacaaaaaa gcttctaatg tgatacagga
     1081 acctcatatt ccgtatcatg ttagaagctt ttaatgtcta aagaacatct acattttatc
     1141 atattttctg acttattaaa cttttatata attaaatatt tcttaatttt ccaaaatagt
     1201 gataaatttg tgaaatacat cacaaatccc tttatttatt tggaaattca tgtaatatta
     1261 gacttgtaag aagttaataa atagagagag acgagagagt ttatataaat actatataaa
     1321 cattggagtg atgattatga gaaaagagat tgaagcgctt attttctcag acgtaagtag
     1381 ctatgatatt tacgtgaaca ctggtgtaaa tcaaggatta attggtgaca tcaaagatgg
     1441 ttacctaact attgattcta tgccttacat tgatgctgag cgtttgtatc actttgctat
     1501 ggaacgtaaa tcgttagtca ctaactagtt cttattgcca atgattacta cccctagtcg
     1561 gcggcaattg aagtgtgatt gatgtaactt gccctcgttg gtgagcaatt gagggcagac
     1621 ccctttaatt aagtaaaccc taactcccca caaatctgga acgatactaa aagccacgtc
     1681 ctatattgga tgtggcttta gtcatactta tattattttt aaaacgatta cctacaagat
     1741 ttacatataa aattctatca tgtctgctat atataaacga aatgatatta gaatccttca
     1801 ttttcaactt gtttaaaatt acgtttaatg ccgttataag aatatacaac attatataat
     1861 tttacttggg cgtcatggac acttgagaag ggcattctta catcgtccat atttttaact
     1921 tcgtgtcgaa atgctttttc agctttttgt ctttctgcaa gagacatact tagccattct
     1981 tctttgctaa ataaatcttc gaatcgaaaa tcaaatgcgg ttcttttact ttctgctctt
     2041 aatgctaaaa cacgctcatg atatgtcacg ttttattcct ccctctaatc attcaatcga
     2101 atgataaatc acagtattaa ctttattact aatataatgt ttttacaata aagaagtgac
     2161 gatttttcaa catttttaaa aataaaaaat ataagcaacg attaattgta tgtttcgaat
     2221 ggcttgtaat gtaatttatc tatgaaaatg aagcgagtta ttatgaccgt tatatgaatt
     2281 gtgatacgct ttttaaaagt aatatgtatt tatgatttac cctgaaaaaa taaaaagaat
     2341 caaaggagtc gatcattatg acagtattaa cagataaagt agcagtagtt acaggtgcag
     2401 gtagtggtat tggagaagca attgcaacat tactacatga agaaggggca aaagttgtct
     2461 tagcaggtag aaataaagaa aaattacaaa acgtagcgaa tcaattgtca caagatagtg
     2521 tgaaggtagt gccaacagat gtaacgaata aagaagaagt cgatgaattg ataaaaattg
     2581 cacaacaaac attcggtggt ttggatattg ttatcaatag tgcggggcaa atgttgtcgt
     2641 ctaagattac tgattatcaa gtagatgagt gggatagtat gattgatgtg aatatcaaag
     2701 gcactttata tacggcacag gctgcattac caactatgtt agaacaatca agtggccatc
     2761 ttattaacat tgcatctatt tctggctttg aagtaacgaa aagtagtacg atttatagtg
     2821 cgacgaaagc agcagttcac actattactc aaggattaga aaaagagttg gcaaagacag
     2881 gcgttaaagt aacaagcatt tctccaggaa tggtagatac agccataact gccgcataca
     2941 atccaacaga tcgtaaaaaa cttgaaccac aagatattgc agaagcagta ttatatgcat
     3001 taacacaacc aaagcacgtt aacgtgaatg aaattacagt gcgtcctgta taaaaaggtg
     3061 tttgaggtct ggggtatagg catttaatgt aatagctatc gattttgtta atgttagtaa
     3121 ggcaataatt acatgagggc ataataggca attattcctt gtacaattct cagtaattgc
     3181 tgattttaac aatcaagtta atgaaggcga cgatgttagt gttatggttt ataatctgta
     3241 atgttcataa ttatagatta taaaaatttt ttcattttcc accacatttt aggagttact
     3301 ttaatgaaca aaaaacatgt ttttataatt attggtgtca ttttatgtat atgtatagtt
     3361 gcatcagtca t
//
LOCUS       DANRDI010000215         3357 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.217, whole genome shotgun sequence.
ACCESSION   DANRDI010000215 DANRDI010000000
VERSION     DANRDI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.217"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1386)
                     /locus_tag="PZ377_002639"
     CDS             complement(<1..1386)
                     /locus_tag="PZ377_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthetase family
                     protein"
                     /protein_id="HDM6359492.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFIL"
     gene            complement(1867..3075)
                     /locus_tag="PZ377_002640"
     CDS             complement(1867..3075)
                     /locus_tag="PZ377_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDM6359493.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(3068..3235)
                     /locus_tag="PZ377_002641"
     CDS             complement(3068..3235)
                     /locus_tag="PZ377_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359494.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(3210..>3357)
                     /locus_tag="PZ377_002642"
     CDS             complement(3210..>3357)
                     /locus_tag="PZ377_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDM6359495.1"
                     /translation="KNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHFEER
                     INQS"
BASE COUNT         1320 a          556 c          428 g         1053 t
ORIGIN      
        1 taaaataaat aaactgaata aaagcattgc aactatattt ctcattctta ctgggcgttt
       61 caacctacgt gctctacgga aagctacaat aagcagaaca aaaataatag ctaaccatgt
      121 tattaaaata tatgaagcgt aagtgaagaa tgtcgccact gtgattaata aaattgaaat
      181 catagcaaag ataatggcac gtctactttg cttataaata ccaactacat tcagtaaaag
      241 taataaacaa gcactagtat gaattgccaa tagaatataa tacgttaagt gatttccatc
      301 atataaagca tcgtaaacaa tcgttaagtt atttacaaaa aagatcatac ttgtaaagaa
      361 tactaaaatt gctaatgata atgatggaat tttagcaata atatcctttt gataagacat
      421 taaaaatgac gtaacatctt tagcaggaat aaagtattta gatccctcaa tgtacttctt
      481 agctgatgta ccaaattcaa atgatgataa aattaatgca ataattaccg gtacaaaata
      541 gtacgcaaaa cgatatagaa gtagcattaa taatactttt tcctcaggga cacctaaagt
      601 tttaaatcct agtaatacaa ctaaatcgaa agcgccgaaa ccaccaggaa taaagctgac
      661 taaacctgat aatgcagcaa tgataaatat tgcaataaag gacatgaatg atacatgagc
      721 gtcaacaatt acaccacaga aatataatac aactgcagct gctaaccatt caacacacga
      781 cactaaagtg cagtacaatc ctacaaaacg attgttttta tcgggtggtc taaccattga
      841 ataaataatg aataatggta agaaaaatga cactacatat aatacccatc ttacccatgt
      901 aatcttatct aagattaaag atgcatcgaa aacatggaat acaattagca atgataataa
      961 gcttaaacct gtcaacattg aaataagtat taaggatata aagtgaacta atttcttttt
     1021 atcatgcgta tagtttttat aaaccattgc tctaacgcct gcaccaataa agccaccgaa
     1081 acctacaatc gcattcaatg cattgatgat ataacttact cttaaaactt tgcctaagga
     1141 tatatccatt tttaaagctc tagataaaat cacatcatac attgatagaa taacaagcga
     1201 tgccccacca ccaataaata gtaacactaa ggacatacgg ttaatcttac taaattcaac
     1261 caacgtatct ttaaagttaa taccagataa ctcccgatac aatgtgattg ctacaaaaat
     1321 aaataaagct gtagcaaacg taatttttaa gattgaaaat attttgtttt taacttcctg
     1381 attcattttt tcacatcaat tctaattatt tctgttataa atcaaaatat atcatataaa
     1441 ccataaaaat agcatatcat ttacctttga tttatataac acttttgtca tattgatatc
     1501 tgtattaaga agtaaataga aaatatttct atctcaaaca gaacaatatg gttatctata
     1561 ctgattcata tttaacataa agaaaatgat gaatcaatac ttatgaaaaa tattattaat
     1621 taacagccga ttatgagtgc ttcttaatat attaaaaata ccacttattt ttattttcga
     1681 cataataatg aagtactaat caagtggcaa atactcgttt cattatgcat gtgctttata
     1741 aacggctaaa ctttcgtaaa aatttataag ctaaacatgt gctgataatc ataaatgtaa
     1801 ctgcccaaca ttataagcac cataaaaaat accaacaaac atatagtcat tggtattttt
     1861 tattacttat tcaactattt tattactttc tttaatatca tattcaccta catcattacc
     1921 atagaaacga ttgtatcgac gtttaaataa ttggaataaa tgagtaacta atacttttaa
     1981 aatagcatat cccggaatac ctaaaataac gcctacaata ccaagcaatt tgcctgcaca
     2041 cagtaaaatg aaaatgattg taagtggatg aatcttaagt gttttaccca tgatatttgg
     2101 tgaaatgaag tgcccttcaa caaattgtac taaagtccat actactgcta attttaagag
     2161 catccacggc gatgttatag cagctattac aatagctgga gaaatcgcta tagtaggccc
     2221 taaatatggt acaacacttg taactgccgc aatactagct aatactaagc tatatttcaa
     2281 cccgataacc gaataaccga taaacaacag tataccgata cagaatgaaa cgataatttg
     2341 tccttgaatg tatgaaccaa cttgaacact cattttttca agtagatcat gaaaatcttt
     2401 tcggaatttc ggtggcataa tattcgttga aaattctttg aagtgatgtc catctttaag
     2461 cataaagaat agtacaaatg gtgttgtgac aatcacaacg ccaatattag caatcgtttc
     2521 tgcaaacgtt gcaattttag acccaaagcc atctgtaaat tcacttaaca tagatggtat
     2581 tttcttaggt aaagaattta accaatcatt aatttgacta taatacgaag agaaaaacgt
     2641 attatttgct attttatcaa tagaattaat taatttttct agatattgcg gtgaattttt
     2701 aactaaacta tctacttgcg aaccaataat aggtatcaat aaattaacaa ttaacgtaat
     2761 aacacctact acagctagat aaataataga aatacctgca actcttggta ttctataacg
     2821 ttccatcata tttacgattg ggttaaatag atagaataga attaaagaga cgatgatagg
     2881 tgctgctatc gtcttaaaaa cgatgataaa aggatcaaaa acatatgata ctttttcgaa
     2941 aataaaaata acaataccca acaatactag cgctattaat gagaagacta aatcattccc
     3001 accaacaaac ttcatatacc gtgattcggt aaattttaaa aaattctttc ttatattctt
     3061 ttcattttca ttcaattata gattcaccac cacttaaaat attcataaga tgttgtataa
     3121 aaacagtcca cttatgtaaa attactaaca tcaatgatac catacaaata ccctttacag
     3181 taacgaaacg cccgtgtaat acacttaatt taggattgat taatcctttc ttcaaaatga
     3241 tgaatgtgta atgttaaata tataatttca gactcactga tattaacatc aaattgtttt
     3301 tgtatcatag ttaaaatttt atatgctgtg ttatagcaaa tcggatagtg attttta
//
LOCUS       DANRDI010000216         3347 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.218, whole genome shotgun sequence.
ACCESSION   DANRDI010000216 DANRDI010000000
VERSION     DANRDI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.218"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            110..385
                     /locus_tag="PZ377_002643"
     CDS             110..385
                     /locus_tag="PZ377_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDM6359496.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            699..1925
                     /gene="ftsW"
                     /locus_tag="PZ377_002644"
     CDS             699..1925
                     /gene="ftsW"
                     /locus_tag="PZ377_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDM6359497.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            2479..>3347
                     /locus_tag="PZ377_002645"
     CDS             2479..>3347
                     /locus_tag="PZ377_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDM6359498.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFI"
BASE COUNT         1165 a          431 c          613 g         1138 t
ORIGIN      
        1 attttaacac aaattgctaa cgacacattt taaatctact tgctttttaa tatttagtaa
       61 gataaacttt tagtaagact tgagaattta tatagagggg gagcgtgtca tggcgaaaca
      121 agcaacaatg aaaaatgcag ctttgaaaca attgactaaa gatgctgatg aaatcttgca
      181 tctgattaaa gttcaactag ataatttaac attaccttca tgcccattat atgaagaagt
      241 actagataca caaatgtttg gacttcaaaa agaagttgat tttgctgtta aattaggttt
      301 agttgaccgc gaagatggca aacaaattat gttacgtctt gagaaagaac tttcaaaatt
      361 acatgaagct tttacacttg tttaaggttt aatatataag catattaaaa tggcgtgaga
      421 tatcgacaat tgagctgcgc cgattttata tttatagttt aattactgat taaagtggct
      481 gttatgttgt cttcttgatg acatgtcagt cacttttatt gggttttagc aattcacgat
      541 attaagtaaa caaatattct ataaattacc tcaagagacc tgttaatatt tgtgattatg
      601 actgtgtcat gtatataata agtaacatgg atttttaaaa ttaaagtata aaacgaaatc
      661 atttgaagta taaattgtag aattggatga ctgaatatat gaagaatttt agaagtattt
      721 tacggtatat tggtaaaacc tcaaagttta ttgattatcc gttattagtt acatatattg
      781 tattgagttt aattggatta gtgatggtat atagtgcaag tatggttcca gcaactaaag
      841 gcacattgac tggtggtatc gatgttccag gaacgtattt ttacaaccga caattagcat
      901 atgtcataat gagttttata attgtatttt ttattgcatt tttaatgaat gttaaattac
      961 tgagtaatat taaagtgcaa aaaggtatga ttataactat cgtctcacta ttattactga
     1021 cgttagtaat aggtaaagat attaatggtt ctaaaagttg gataaactta ggatttatga
     1081 acttacaggc atctgagtta ttaaaaattg caatcatatt atatatccca tttatgatca
     1141 gtaaaaaaat gcctagagta ttaagtaaac caaaattaat tttaagtcct attgtattag
     1201 cattaggttg tacgttttta gttttcctac aaaaagacgt agggcaaaca ttactaatat
     1261 taattatttt agttgcgatc attttttatt caggaattgg ggtaaacaaa gtcctaagat
     1321 ttggtatacc agcagtgcta ggattcttag tagtatttgt cattgcatta atggctggtt
     1381 ggttaccaag ttatttaact gccagattta gtacgctaac agatccattc caattcgaat
     1441 caggaactgg ataccatatt tccaattcat tgcttgcgat aggtaacggt ggcgtatttg
     1501 gaaaaggatt aggaaatagt gcaatgaaat tgggctattt accagaacca catacagatt
     1561 ttatttttgc aattatttgc gaagaattag gtttaatcgg aggattgcta gttattactt
     1621 tagagttctt tattgtatat cgtgccttcc agtttgcaaa taaaacatca tcatattttt
     1681 ataaacttgt gtgtgttggg attgccacat actttggaag tcaaacgttt gtaaacattg
     1741 gcggtatttc ggcaacaatt ccattaactg gtgtgccatt gccatttatc agctttggtg
     1801 gatcatcaat gattagttta agtattgcta tgggtttact tctgattgta ggtaaacaaa
     1861 tcaaagtaga ccagcaacga aagaaacaac aacaaaaagt tgatataaga agacaattta
     1921 attaaaaaaa tactagccaa tatttagtac gagatgactt ctgtcattaa gcaatgctaa
     1981 acattggcta tttatatact ctttgtcaaa ttggactgat gagttcaata acggaagtta
     2041 ggcaaccaaa caacgcttaa cttccttttt gttttgatat tacgagatta ctcatgatta
     2101 tcaaatttta taaacaaaac aacgtataat tataactaaa ttttatgact aaaaattact
     2161 aaatgtggtg gcgaaattag aatcatgatg ataagataaa gttatcgcta ttgataataa
     2221 atgttgatgt tttgcaattt tatttttaat tttttagagt tgtagaaatc gaaaatgttt
     2281 cacaacaaaa acgtgaacta cctgttgttg catagtcatt tttgaaatta ttatttctga
     2341 agcggaggtt ttaattgtct caagaatgtt aaattttgaa aatttgaaac ataactatag
     2401 caaaacagag cgctttaaga taaaatttta ttatctaatt agttagaaaa ttctaatagt
     2461 ttgaggagtg tatcgctatt gaaacaaata aaaaagttac ttgttgctaa ccgtggagaa
     2521 attgcaattc gtatattcag agcggcggca gaattagaca tcagcacagt tgcaatttat
     2581 tcgaatgaag acaaaagttc attacataga tataaagcag atgaatccta tttagttgga
     2641 agtgatttag gtcctgctga aagttattta aatattgagc gtatcattga tgtagcaaaa
     2701 caagcgaatg tggatgcgat tcatcctggc tatggatttt taagtgaaaa tgaacaattt
     2761 gcgcgtcgtt gtgcagaaga aggaattaaa tttattggtc ctcatttaga acatttagat
     2821 atgtttggag ataaagttaa agctcgtaca acggctatca aggcagattt accagttatt
     2881 cctggtacag acggtccaat taaatcatat gaattagcaa aagaatttgc agaagaagct
     2941 ggtttcccgc taatgattaa agccacaagt ggtggcggcg gtaaaggtat gagaatcgtt
     3001 cgtgaagaaa gtgaattaga agatgctttc catagagcaa aatcagaagc tgaaaaatca
     3061 tttggtaata gtgaagttta catagaaaga tacattgata atccaaagca tattgaagta
     3121 caagtcatag gtgacgaaca tggaaatatc gtacacttat ttgaacgtga ttgttcagta
     3181 caacgtcgtc atcaaaaagt tgtagaagtt gcaccatcag ttggattatc atcaacatta
     3241 cgtcaacgta tttgtgatgc tgcaattcaa ttgatggaaa atattaaata tgtcaatgca
     3301 ggtactgttg aatttctagt atctggtgac gaattcttct ttataga
//
LOCUS       DANRDI010000217         3305 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.219, whole genome shotgun sequence.
ACCESSION   DANRDI010000217 DANRDI010000000
VERSION     DANRDI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.219"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1106)
                     /gene="sfaA"
                     /locus_tag="PZ377_002646"
     CDS             complement(<1..1106)
                     /gene="sfaA"
                     /locus_tag="PZ377_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDM6359499.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMG"
     gene            1208..3184
                     /gene="sfaD"
                     /locus_tag="PZ377_002647"
                     /gene_synonym="sfnaD"
     CDS             1208..3184
                     /gene="sfaD"
                     /locus_tag="PZ377_002647"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDM6359500.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
BASE COUNT         1225 a          568 c          491 g         1021 t
ORIGIN      
        1 attcccatta ttgaaaaagt tctaacaatg ccaaacacat ctgcacatat actcattaat
       61 agacttccca atggaatcat acctctatcc atcataataa tactcagtac tttaccacgt
      121 tcataatctt taacattatt ttgaaaataa acgcgatttg tcgtacgtgc ccattgacta
      181 aataacccaa tcaatgtaat acaaatgaac atgatgacaa tattgtgaaa taccacacct
      241 aacaaagcaa tgccaaataa aaatgaactt aaataataca tatttaccat accaatatat
      301 ttaagtactt taggtaaaac tagcgttgca ataataccac caatggcaca catcgtcata
      361 gcgataccaa atatttcaga tttccccgga aatactttgt ttgtcaaaac tggtaaaagt
      421 gtcgtatatg aaaaacctgt cgccataatc aataatgatg ttataaatat ttgtcgacct
      481 tccatatgta atttgaagta atctataata acttttaacg gcatgtatct tgatgcatct
      541 tcaggtattt tagttacttt aaaatgtaat ggtaagcata ataaaactgc gataaaataa
      601 caaattgcct gtgcaagaaa tgttgttggc gcatgataga ctgctaatat gacaccagca
      661 atggcaggcc ctattgaacg acaaatatta atgatgaatg aatgaaatga tacagcttgt
      721 gtagtagata ttttatctga taaatctggt aagattgctt gtcttaaagg tgtttcaacc
      781 gcacttaaaa tccctcttaa tgtcgcatat ataataatga cgctaattgg aattgcagtg
      841 aaactatacg tgagcacaca taaaattgca gttactaaaa atgatgatga aattgtaatt
      901 ctcagcaatc gccctttatc atatttatcg gcaattgccc ctgcccacac acttagtaat
      961 aatattggta caagtctgca aaaattgaca atccccagat aaactgcgtt atgataagtg
     1021 gtaagtacaa accaatttag ccctatttgt ccaatccaat tacctagaaa tagtagaaaa
     1081 gaactgctaa aaaaatattt tgtcataaaa cttacacccg cattctgttg ataataagaa
     1141 tcattatcat taatatggat tatacaaatc tatcttgctt ttgtaaaata tttcatagag
     1201 gtgaattatg aacttgaact taatttttaa agaacagaca ttaaaattta acaaagaaga
     1261 acaagaaaca tatttatttt tacaacaaca taatagcgat tgggctaata tttttaaaga
     1321 gatgatttta caaggaagag ataaagtcac tcagagattg gtcacatcaa tgcatcgcga
     1381 aaacttagtt aaagcgcgca ctcaaagtaa gaaaatattg agtcgtgatt tgattatgtt
     1441 agatatctca acgactcata tactagaaat ccagttccca caagctaagc aaacattata
     1501 tgctcctatc actggagaac acgcctttga tcgaattgat gttgaaggcc ctttctacat
     1561 taaagatgac attacaaata cgattacacg agtacaccat ccaaatgaaa ttcttgagtg
     1621 tattttaatt gaagcaccag atttaaaaaa tgctgctagt gatcaatttc agcaagattt
     1681 aataaacagt gcaactaata tgacatttgc aattagctat caagcactgt caatgcaaca
     1741 tgattctgca cctttattta atattatcga aaatagcgaa gatagttact tgcgttcaga
     1801 gcaagccgtt attgaaggtc atccgctaca tcctggggct aaattacgta aaggtttaaa
     1861 tgcattacaa acattcctat attcttcaga attcaatcaa cctattaagt tgaaaatagt
     1921 actgattcac agtaaattaa gccgtactat gtcactgtct aaagattatg atacaactgt
     1981 tcatcaacta tttccggact taattaaaca attagaaaat gaatttacgc caaagtttaa
     2041 ctttaatgat tatcacatta tgattgttca tccatggcaa ttagatgacg tattacattc
     2101 tgattatcaa gcagaagtag ataaagaatt aatcattgaa gcaaaacata cgttggatta
     2161 ttatgctggt ttatctttca gaacattagt tcctaaatac ccagctatgt cgccacacat
     2221 caaattgtcg acaaacgttc atattacagg tgagattcgt acattatcag aacaaacgac
     2281 gcacaatggt ccactaatga cacgtatttt aaatgatatt ttggagaaag atgtcatatt
     2341 taagtcctat gcatcaacaa ttattgatga agttgctggt attcattttt ataatgagca
     2401 agacgaagca gactatcaaa ctgaacgaag tgaacaatta ggtacattat ttagaaaaaa
     2461 tatataccaa atgattccac aagaagtcac accattgatt ccttcaagct tagttgcaac
     2521 ctacccattt aacaatgaat cacctatcgt gacattgatt aaacgttatc aatcagctgc
     2581 atcattgtct gattttgaat catcggcgaa atcgtgggtt gaaacttata gtaaagcatt
     2641 gcttggtctc gtaatcccac tagttactaa atatggcatt gcattagagg ctcacttgca
     2701 aaatgctatt gcaacatttc ggaaagatgg tctattggac acgatgtata ttagagactt
     2761 tgaaggttta agaattgata aagcacaact aaatgaaatg ggatacagta catctcattt
     2821 ccatgaaaaa tcaagaattc ttactgattc aaaaacgtct gtgtttaaca aagcctttta
     2881 ttctaccgtt caaaaccatt taggcgaatt aattcttaca atctctaaag catcaaatga
     2941 ttcaaactta gaaagacata tgtggtatat cgttcgtgat gtactagata acatttttga
     3001 tcaacttgta ctttcgacac acaaatctaa tcaagtaaat gagaatcgca taaatgaaat
     3061 taaagatacc atgttcgcgc catttataga ttataagtgt gttacaacaa tgcgtcttga
     3121 agatgaggca catcattata cttatattaa agttaataac cctttgtatc gagaaaataa
     3181 ttaaggttat agaattttat taatcgtgag ctcaaggatt acattaactt attaaataca
     3241 atctatacaa atgagagaat aatacatttt ccatattcaa attattaaaa atcacaaaaa
     3301 acagg
//
LOCUS       DANRDI010000218         3290 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.220, whole genome shotgun sequence.
ACCESSION   DANRDI010000218 DANRDI010000000
VERSION     DANRDI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.220"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            223..1206
                     /locus_tag="PZ377_002648"
     CDS             223..1206
                     /locus_tag="PZ377_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDM6359501.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1397..2323
                     /gene="pstC"
                     /locus_tag="PZ377_002649"
     CDS             1397..2323
                     /gene="pstC"
                     /locus_tag="PZ377_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDM6359502.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2325..3242
                     /gene="pstA"
                     /locus_tag="PZ377_002650"
     CDS             2325..3242
                     /gene="pstA"
                     /locus_tag="PZ377_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDM6359503.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
BASE COUNT         1170 a          490 c          592 g         1038 t
ORIGIN      
        1 tttttgtatt catatttaaa tttcgatgat ttttcaggaa gcattttaat tttactaatg
       61 aagcaatatt ttttagatta acaaaaatta atatttacat tttcttaaca attttttatg
      121 taacatttac agtttctaaa aatgaggtta ataattcaag gttaagataa agatgtaatc
      181 aatacaaata ctatttgttg ttcatacagg gaggatattt caatgaaaaa atggcaattt
      241 gttggtacta cagctttagg tgcaacacta ttattaggtg cttgtggtgg cggtaatggt
      301 ggcagtggta atagtgattt aaaaggggaa gctaaaggtg atggctcatc aacagtagca
      361 ccaattgtgg agaaattaaa tgaaaaatgg gctcaagatc actcggatgc taaaatctca
      421 gcaggacaag ctggtacagg tgctggtttc caaaaattca ttgcaggaga tatcgacttc
      481 gctgatgctt ctagaccaat taaagatgaa gagaagcaaa aattacaaga taagaatatc
      541 aaatacaaag aattcaaaat tgcgcaagat ggtgtaacgg ttgctgtaaa taaagaaaat
      601 gattttgtag atgaattaga caaacagcaa ttaaaagcaa tttattctgg aaaagctaaa
      661 acatggaaag atgttaatag taaatggcca gataaaaaaa taaatgctgt atcaccaaac
      721 tcaagtcatg gtacttatga cttctttgaa aatgaagtaa tgaataaaga agatattaaa
      781 gcagaaaaaa atgctgatac aaatgctatc gtttcttctg taacgaaaaa caaagaggga
      841 atcggatact ttggatataa cttctacgta caaaataaag ataaattaaa agaagttaaa
      901 atcaaagatg aaaatggtaa agcaacagag cctacgaaaa aaacaattca agataactct
      961 tatgcattaa gtagaccatt attcatttat gtaaatgaaa aagcattgaa agataataaa
     1021 gtaatgtcag aatttatcaa attcgtctta gaagataaag gtaaagcagc tgaagaagct
     1081 ggatatgtag cagcaccaga gaaaacatac aaatcacaat tagatgattt aaaagcattt
     1141 attgataaaa atcaaaaatc agacgacaag aaatctgatg ataaaaagtc tgaagacaaa
     1201 aaataataag acgcaatttc aaatgtgtct tgaaacatga ttttgatggt gaatcattat
     1261 ttagagtaca aagcttgatt tatcgagacg ctgattttga cattcagtta gtctacaagc
     1321 ttatcaactt aaaatagtgg ttcatcatta ttttacaaat ctaattattt tgggagtaat
     1381 agaaagaggt ttgattatga cttcatctac taatgttaaa gctttaatcg aaaaaaataa
     1441 taataaaaaa ggaaagcata atgacaaaat tataccagtt attttagccg caatttcagc
     1501 gatttccatt ttaacaacac taggtatatt aatcacattg cttttagaaa ccatcacttt
     1561 tttcaccaga attccaataa ctgaatttct attttctact acttggaatc ctaccggttc
     1621 agaccctaag tttggtatct gggcattgat aatagggact ttaaaaatca cagttattgc
     1681 gactatattt gcagttccag tcgggcttgg tgcagcaatt tatttaagtg aatatgcaag
     1741 tgaccgtgca cgacgcataa ttaaaccgat attagaaatt ttagcaggaa taccaacaat
     1801 tgtgtttggt ttctttgcat taacctttgt tacaccagta ttaagatctt tcataccagg
     1861 tcttggagag tttaatgcta taagtcccgg cttagttgtc ggtattatga ttgtccctct
     1921 catcacaagt ttgagtgagg atgcaatggc atctgtacca aataaaattc gagaaggtgc
     1981 ctatggactt ggagcaacta aattagaagt agcaactaaa gtcgtacttc ccgcagcaac
     2041 atcaggtatt gtagcttcaa tcgttctcgc gatttcaaga gcaattggag aaacgatgat
     2101 tgtatcatta gcggcaggta gttcgccaac agcttcatta agtttaacaa gttcgattca
     2161 aacaatgact ggatatattg ttgagatagc gacaggtgat gcaacatttg gatcaaatat
     2221 ttattacagt atttatgctg tagggttcac actatttatc tttaccttaa tcatgaattt
     2281 actttctcag tggatttcta agcgttttag ggaggagtat taatatggaa acgacagata
     2341 ataatagaca atcactcgtc gatcaacaac ttgtccaaaa acatttatca tccagaacgg
     2401 ttaaaaataa agtgttcaaa ctcatatttt tagcatgtac attattagga cttgtcgtac
     2461 ttattgcgtt gttaactcaa acattgatta aaggggtaag tcatttaaat ttacagtttt
     2521 tcactaattt ttcttcttca acaccatcta tggctggcgt taaaggcgcg ttaatcggtt
     2581 cactttggtt aatgttaagt atcattccat tatcaatcat cctaggaata ggtacagcta
     2641 tatacttaga agaatatgcg aaaaacaaca aatttactca gtttgttaaa atcagtattt
     2701 ccaatttagc tggtgtacca tcagttgtat ttgggttatt aggttatact ttgttcgttg
     2761 gtggtgcagg gattgaagcc ttgaaaatgg gtaacagtat attggcagca gcgctaacaa
     2821 tgaccttact gatattacca attattattg tttcaagtca ggaagcaatt agagctgtac
     2881 ctaactcagt acgcgaagct tcttacggct taggtgctaa taaatggcaa acgataagac
     2941 gtgttgtctt accagcagcg ttacctggta ttttaactgg attcattttg tctctttcaa
     3001 gagcactggg agaaacagcg ccacttgtgc taatcggtat accgactata ttattggcaa
     3061 cacctagaag tatattggat caattttcag cattacctat ccaaatattt acttgggcga
     3121 aaatgcctca agaagaattc cagaatgttg catcggcagg cattatcgtt ttactagtta
     3181 tcttaatctt aatgaatggc gttgcgatta ttttacgtaa caaatttagt aaaaaattct
     3241 aatttaaaca atcaatctca tttatctatt aaaaagggag ttttaaatat
//
LOCUS       DANRDI010000219         3266 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.221, whole genome shotgun sequence.
ACCESSION   DANRDI010000219 DANRDI010000000
VERSION     DANRDI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.221"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..473
                     /locus_tag="PZ377_002651"
     CDS             <1..473
                     /locus_tag="PZ377_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HDM6359504.1"
                     /translation="YGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKLETISEPDE
                     NGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSVTEVKVSVG
                     ETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIEKATD"
     gene            complement(613..1524)
                     /locus_tag="PZ377_002652"
     CDS             complement(613..1524)
                     /locus_tag="PZ377_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDM6359505.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            1964..2875
                     /locus_tag="PZ377_002653"
     CDS             1964..2875
                     /locus_tag="PZ377_002653"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDM6359506.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            2900..>3266
                     /locus_tag="PZ377_002654"
     CDS             2900..>3266
                     /locus_tag="PZ377_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDM6359507.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFC"
BASE COUNT         1145 a          487 c          560 g         1074 t
ORIGIN      
        1 aatacggaaa cttatcgtta cttgatacgc ctacattctt ctttggaatg cgtaatggtg
       61 aaacagtaga aatcgaaatc gataaaggta aacgattaat tattaaacta gaaacgatta
      121 gtgaaccaga tgaaaatggt aatagaacga tttactatgc gatgaatggt caagcgagac
      181 gtatttacat taaagatgaa aatgtgcata caaatgcgaa cgttaagcca aaagcagata
      241 agagtaatcc aagtcatatc ggtgctcaaa tgccaggttc agtaactgaa gtcaaggtta
      301 gtgtaggtga aactgtgaaa gctaatcagc cgttgctaat tactgaagct atgaaaatgg
      361 aaacaacaat tcaagcacca tttgacggtg tgattaaaca agtaactgta aataatggtg
      421 acacaatagc gacaggcgat ttattaatcg aaattgaaaa agcaactgac taaaatgatt
      481 aaaataaaac gagattacac aacaattgac gtgtaatctc gtttttacat ggcttaggtt
      541 taattttaaa tattgatata aaaaaccgta attttaatgg gcgtgccatt aaaattacgg
      601 tttgcttttt tgttattgct tgtcacttct tactgatcgt aacattagca tgataaagta
      661 tgttgtcata ccaaataaat aagtgataaa taatgcatgg aaaagtgcga ttatcaggtt
      721 aacatttgtc ataatagata atgcacctgt gataacttgt aatataacaa gtataaatgc
      781 agctgtataa ccataatgaa cagtacggtt atttggataa tttttaacag cgtgaatata
      841 agtaatcata ataatcgtaa acactataaa agccatgata cgatgcgtga gttgaaccca
      901 atcttgttct gaatgtggta caagatcgtg gaatggcaat ggccaaccac catatgctaa
      961 acttgcatcc gcatgtctca ctagtgcacc agtataaaca ccacaataaa tgatgattgc
     1021 cattaaccat gttaaacgtc ttaatggctt tttgatatat aattcgtcag cttcatattt
     1081 ttgatctata gagaaaataa tcaatgttat taaaaataca gatgagaaac tgattaatga
     1141 tataccaaag tgcaatgcta aaacgtaatc gttttgttgc caaataacag cagcagctcc
     1201 gattaatgct tgcaataata agaatccaac actaatgatt gataaaggtt taatttcttt
     1261 aatatagcct atatgtttcc atgcagtgat aactaaccat aagaccatta ataaagacaa
     1321 agctgaaacg gctctatgac ttaactcaat aatcgtatca ataggaaaga attctggaat
     1381 caacgcacca tgacatagtg gccaagaaga accacaacca tcagctgatc cggttttggt
     1441 aactaaggct ccaccaagtt gtacaaatgt catcattaac gttgctacga cacctaacca
     1501 ttttaaattc tttttgccaa acaatttata caccccatca taaaaaaacg catcactgag
     1561 aattagaatt aatagccgga tttgagatga tactataaat agacctgatt gtaggatatg
     1621 aaaagatggg atccaacgca ggaaatatga tttaatttta caagtattgt atgtggacga
     1681 cataaaaaga acttaatcgt aatgattttt ttattgggat aattatgtaa tccacgttta
     1741 ctcaaatttg aagcttaaat attaatctct tttagatgca tgattctact tattaaaatt
     1801 ttctaacata tacattatag caataaatta aatgttattt gtgtcacaaa attgacattt
     1861 tctcttgtcg ttaatttgtc acaatttaat tttattaaac ctagtctttt tatgcaatct
     1921 aagatatcat aatcttatat gaacaaattt aaggaggggg attatgagca aagagcatac
     1981 tttgtcacaa aatattagca gagttaactt caaagaattg caacagataa ttaaaatggg
     2041 acttgttcaa ggtaacttaa ttccggcttt tgcgggcgca tggctagcag ttgtaatgac
     2101 aaatcattcc ttcttatcat caatacctca aattttatta atgctatttg gatctacttt
     2161 aattatgggt ggcgcatgtg cgttaaataa ttattacgac caagatattg atcgtattat
     2221 gcctagtaaa caaaatagac caactgtaaa taatagaatt acagatcaaa atttattact
     2281 attaagtttt ggtatgatgt tagttggaga aatttgtttg tttttattga atataccatc
     2341 aggcgtactt ggtcttatgg ggattgtagg ttatgtgtct tattactcaa tatggtctaa
     2401 aagacataca acatggaaca cagtgattgg gagttttcct ggagcagtac caccactaat
     2461 tggatgggtt gcaattgaag gacaaattag tttaacagcg attgcgctgt ttttagttgt
     2521 attttgttgg caaccaattc atttttatgc cttagctatt aaacgtaaag atgaatatgc
     2581 acttgcaaat attccaatgt taccatcagt taagggcttt aaacgtacac gtgtcagtat
     2641 gtttatctgg ttgattattt tattgccagt acctttatta ctaataaatt taggtgtcgt
     2701 attcgtagtg ttagctacat tattaaattt aggatggatt gcattaggtt taacaacatt
     2761 taagaaaaat tcagatcaaa caaaatgggc aacacaaatg tttatatatt cactaaatta
     2821 tttagtgatc tttttcgtgt tagctgtgat tgtttcatta cttactttga tctagattaa
     2881 ttaagttagg atgaaaaata tgggcgttcc aattttacca acgattagta cgacatgtat
     2941 tgtcattagt gcaattttaa ttgccattgg ttggagactt atttggaaaa gggaaataaa
     3001 taaacacaaa aatgttatgt tagctgcagc tgtttttgct ttaacttttt tcttaatcta
     3061 tgcaagtaga acgattttta tcggtaatac agcatttggc ggaccagcat caattaagaa
     3121 atattatacg attttcttat ttttccacat taatttagca acaattggtg gtattctagg
     3181 cctagttcaa attattactg catttaaaga taaatataat gtacaccgca aattcgggcc
     3241 attcgcttca gttatatggt tctgta
//
LOCUS       DANRDI010000220         3234 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.222, whole genome shotgun sequence.
ACCESSION   DANRDI010000220 DANRDI010000000
VERSION     DANRDI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.222"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(69..2252)
                     /locus_tag="PZ377_002655"
     CDS             complement(69..2252)
                     /locus_tag="PZ377_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDM6359508.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(2249..2926)
                     /gene="recU"
                     /locus_tag="PZ377_002656"
     CDS             complement(2249..2926)
                     /gene="recU"
                     /locus_tag="PZ377_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDM6359509.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT          977 a          563 c          518 g         1176 t
ORIGIN      
        1 gaagaaccta aaaaaatcct cggctacacc ataaatatgg tataactgag gattttatgt
       61 tgagtggatt agttgaatat acctgttaat ccaccgctgt ttctagattg ttgagatcta
      121 gtattgttat ttgattgtgc agtaccactg ctgtttgctg atgtgtcact accaccgtgt
      181 gtactacgat ttgtagtgtt gttatcttga ctaccagaaa cattgattga tgggatacta
      241 ccacttactg agctaggacg tttaaagtct tcgccatctc tagatgaaat ttttgacatc
      301 acattttcat ataagaactg tggatattct tgttggctat gtcccacaaa tgagttttca
      361 ccatattgtt taactttact gaagcccatc cacactgaca tagtgtattg aggtgtaaag
      421 ccgttaatcc acacgtcttt cgctgcatta tcaggtaaat tatattgtga ataagtttca
      481 gcaccgtaag taccagtacc tgtcttagca cccatattta ctccagatac accatggcca
      541 tatgcagaac catatggttt aaatgtacct tttagcatct cagctaacat gtatgcagtg
      601 taatcactca tcgctttatg gctagtatga tcgtattcga ttgtttcacc atcacgagta
      661 actacttttt gaattgaatg cgcgttgtta taagtaccac cgttagcgat tgcagcaaat
      721 gctgatgcta attgtgttgg tgagaattct gaagcagaac caccaagtac ttcagatgga
      781 ccaatatcgc cttcgtagtt taagccaagt ttggcagcga atttcttagg tgcatcatta
      841 ccagcatttt gcttaactga ttgccaagct tttaaagctg ggatattgaa actttgtcgt
      901 aaagcatcat aaatagatac agtaccgtga ctcttcgtat catagtttct aaatgtagaa
      961 ccatcaactt gatatgaaga ttcatcttga atcgcatggt ttgttgccca tttcatattt
     1021 tcaatggcag gtccatacgc taagaaaggt tttaaagatg aaccagtagg gtgaggatct
     1081 gttgcttggt ttctgttaac gacgtctttg aaatcacgtc caccagatat agcaactaaa
     1141 ccaccagttt tactatcaag aattgttgca ccaacttgtt ggtctttatt cttgtagaag
     1201 ctaccattat caacatcatt ttgtaatgtt ttttgaacat ctttatccat gtttgtataa
     1261 attttaatac cactttgtaa tacattacct aaattttcat ctttgaatgc tttattattc
     1321 attaattcag attttacaaa gttaacgtat gaattatact cagaatcttg atttgtatca
     1381 atgttttgac gttcttcagg agtacgattt actaagttcg cttttaaatc gattttctta
     1441 gcatcttccc actgtttatc tgtaatgcgt ttatgataat gcattaagta taaaacagtg
     1501 tttttacgat cttcagcagc ttttggatga tcataaatat tatagttgtt tggaacctga
     1561 ggtaaaccgg ctaaataagc ttcttccgct aagtttaaat cttttaaatc tttattaaag
     1621 taatacttag cagcagcttt aatacctgtt acgccatcag aatagtaaat tttgtttaga
     1681 tatacttgga agatatcatc tttactatac tcttgttcta aacgatatga taagtatgct
     1741 tcttgagctt tacgtccaat agatttatgt tgtgataaaa atgcatcttt aacaacttgt
     1801 tgtgttaatg ttgaggcacc ttcagaacca aatccaccag tcaagttctt accaattgca
     1861 ccgaataaac gtttataatc aagtgcgcca tgttcgtaga aacgattgtc ttcagttgca
     1921 agtactgcgt ctttcattga tttcggcacg tcttttaaat ttacatgctc atgtctttgg
     1981 ccattatcta atgttttaac aagttctccg ttcttgtcat atatctttgc aggaatcgga
     2041 tcttgtaatt tagcttcggt aaaagcaggt gctttccaag cataataagc aaacaataag
     2101 atacctagta aaagaacaac gaaaaatgca ataatcatga agccaataat cttaataatc
     2161 gttctcttca catttctatt cttttttgag ttggatttcc caccattatt accgtttttc
     2221 ttaggctgag aagatccttt gttttccgtc atacgcggtc ctcactttca tctaatatca
     2281 acttatcaac tgcttttaga tagtctaatc ttggttgata ctgataagga atatggtaac
     2341 cattttttcg tatttcatca actgttatag actttttaat attatcttta tatctcttcc
     2401 aaaatacttc gaattttgaa tagggtaaaa gataaacttc atctagcgtt ttaaaacgaa
     2461 tcattaaaaa cacaatacct ttttgttgat atgcattttt catatgttcg acttgatggt
     2521 catgaatatt atttaaagga aaggacgtct tgtttttagt ttcctttgct tcaaaatcaa
     2581 tataataacc ttgataaacg ccgttgtaat cagttgttga aggtgtacga aaataagctt
     2641 cgttaatcac agctttactc cgcttaggat agttgacatt aactatttgt actggcgtag
     2701 gctttttgtg aataactgca atgtcgcttt taagataaaa cgtatttgaa tgttcgatat
     2761 ctttttcaag tgacatacca cgtccaccat actcaatatt actaaaggca gcggtctttt
     2821 tccctccgtc tatagcacta tttttacgat atggtttacc atttggataa ttcataaatt
     2881 tcaccacaca agtttggtag taaacgttgt ttattataca caacattata gtgaaacacc
     2941 aaacttaaat tagtataaat gcgaaagaca caatacacca aagcttattt taacgtgatt
     3001 tcagcattaa cacaataata ttataataca tttaccgtta atgttggaat tttacacaca
     3061 attttaatta attgaaaagt tttgcttata aaaatgaata ctcattacaa tatttttgaa
     3121 ttttgttata aaagtcaaat catgattagt aatttttatt tttgaggcaa gtacaagtat
     3181 gtgttgttaa tcagcagagg tgtatttgat ttaaatgatt ttattagatt aaaa
//
LOCUS       DANRDI010000221         3213 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.223, whole genome shotgun sequence.
ACCESSION   DANRDI010000221 DANRDI010000000
VERSION     DANRDI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.223"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..210)
                     /locus_tag="PZ377_002657"
     CDS             complement(<1..210)
                     /locus_tag="PZ377_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDM6359510.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQA"
     gene            complement(536..1528)
                     /locus_tag="PZ377_002658"
     CDS             complement(536..1528)
                     /locus_tag="PZ377_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDM6359511.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            1762..3138
                     /locus_tag="PZ377_002659"
     CDS             1762..3138
                     /locus_tag="PZ377_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6359512.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
BASE COUNT          956 a          604 c          523 g         1130 t
ORIGIN      
        1 tgcttgattc cagttaccat caggtgtgtg atgataatcc ccattagaat caattgtata
       61 ataagtacca taagatacgt cttgtgattg tgttgacatt tgtgtatgtg cttgttgggt
      121 gtttgtcgtt tctgctgcat ctgcttgatg cgccattgta aatgtagcga agcccatcgt
      181 tgcaatcgct gctgctgtta attttttcat gtataaaaca tcctccatta aagttaaagt
      241 tagttttcaa ttaaactgta ctgcacatac taaaagaatt agacaactga gtaaaggatt
      301 taattctcat tttccaacta tttaatattc ccgaaatgtt ttactaaact cattacattg
      361 tcattacaaa atagccatac attgatatta aaatgacatc tctcactgca ttcgtttaac
      421 cttttataaa ttttcaaaat taacaactaa tcgttcgtcc atgttcgcga ttcaacgcta
      481 atgtatgata gtaatcatcc atcaaatcat atcaaccaaa ttccattatc aatcgctatt
      541 gattgtcatt caactttcta atagtgatat gcttctcagg cttaaaaatc gtcatatctt
      601 ttctattaat taaatcatct gtgagcttta atgctactaa ttcattgctg ccataatact
      661 taatatataa cgttcttgta gttaaattta ttacggtctg atacatcgta tagtgattgt
      721 cagcatcatg cggacgtaca attccaatcg gtatatttac cgcatctaat aaataaaatg
      781 cattcattaa atccatttct ttatcattgt tttgagcaat gtttgctttc ataaatgcca
      841 ttctcacaaa gcgctcagtt gaagtaaatc cacctggcaa tccaaatgta cctgcttcat
      901 tgcctaaagg ttcaatcgtt acaccttcca ataaatttgc tgttgctgga taaggagaaa
      961 tattgatata ttgtcttaaa ttactataat gccaatttaa gtctggatga tttgttaaga
     1021 caccaatagg attatctttt ataaccactt caccctcttt aaatgaaact tcgactgtat
     1081 gtccagttgc atcggaaaca tgataatgca atggcggaac ttcaccgatg tcatttaaat
     1141 atacagctac aacatgtatt tgggatgctt gttgtttcat atcttcaatg cttgttgtat
     1201 atcccaaaat ccatgtcaca atttcatttt gcgtaatatt catcgcgtcc gctttgtgtg
     1261 ttgatccata tgaactataa cctcggaaat attgtgttga aatggcaacg ccatgttcat
     1321 taacaccatc accataaata aaaccttcca tatctgttcc tgtgccaata aagccatatt
     1381 gcgtttggcc tgtcgtgcca gtgcgagatt tccaacgata atttctaggc gtcactgctg
     1441 gcgaaccatc taatggataa tcataatcca tcgtgcgtcc aagaagtact tgattattta
     1501 aagtttgtat tgtgaatcct gtgcacattg ttctcactcc tctgtacctt catttacttt
     1561 aatcactttc aaataaagct gtttcactta aacatactat aaaaaatcaa ttatacaagc
     1621 aattaattga tattcattct caataactgt ggtatgatat gtaaggaaat catgacttat
     1681 gtgtgagtga acgatcatct atacatccgt tcacttcatc tcatgacttt ctatatttaa
     1741 tttttacaag gagtgacatc tgtgaataac acacaatctt caccacgcag taatattatt
     1801 attgcgatta tgttgtctgc attaacatat tggttgtttg cacaatcatt tattaatata
     1861 ggacctctcg ttggtcaaac gtatcaaacc tctcctgccg tgttaaattt atctattagt
     1921 ttaacttcct tcgccacagg tatcttcatg gtggctgcag gtgatattgc tgataaaata
     1981 ggacaactga gaatgacata catgggtctc ataatcagta tgtttgcatc tcttctatta
     2041 attatatcgg acatcactgc actgctcatc atcggtagaa ttttacaagg tctatcagca
     2101 gctatcttgt taccttcaac agttggcgtg ttaaataatc aatttaaagg agaacattta
     2161 agacgagcga ttagttatct aatgattagt actgttggtg gcatcggcct agctggtgtt
     2221 atcggcggtt taattgcctc aaatttcgga tggcaaacga atttcatcat tagtatagtc
     2281 attgctttca ttgccatatt gcttctaaaa ggcacacctg aaaaagtaag tcaacatagc
     2341 caccgtcatc cattcgatta caaaggtatg tcgattttcg ctgttatgat tggtagcttt
     2401 acattattgt taacacaagg attcgaacaa ggttggttta gtacattttc aatcatttgt
     2461 ctgagcattt ttatcatcac tacgttgata ttcatcatca tcgaacgtcg acatgaagta
     2521 ccttttattg atttttcagt attacgcaac cgtccgttca ttggtgcatt tttaaataac
     2581 tttgttttaa atagcggtct aggcgtaaca gtggtctttt tcatatatgc tcaaacacac
     2641 cttggtttat cagctgcgca atctggactt gttacattgc catatgccat tgtggcagtt
     2701 gcgatgattc gtttaggtga aaaagcaaca ttacgtttcg gtggcaaatt gatgctcatc
     2761 attggtccgt tgttccctgt catcggcatc actattatta gcatgactca attattggca
     2821 tcacaatatg tcattgcagt tatcattggt ttcgtcatat gtgcgatagg taatggttta
     2881 gtcgcaacac ctggacttac gattgcaatt ttcagtatgc ctaatgaaaa agttggttta
     2941 gctacaggat tatataaaat gagtggtaca ttaggtggcg cctttggtat agcactaagt
     3001 actacagttt tcagtatgtt acaactaaac tatgcaccaa gtgtagctgc aaccgtaaca
     3061 tttatagtca gcattgtatt gatgatcctt ggctcattgt ctgcatacat gatcattcca
     3121 aaaacagtta aatcttaaat ataatagaag aattatgttt cgaaatatct ttatcacttt
     3181 aaaatgatat acaagaaatc caagaaaaat aag
//
LOCUS       DANRDI010000222         3194 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.224, whole genome shotgun sequence.
ACCESSION   DANRDI010000222 DANRDI010000000
VERSION     DANRDI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.224"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            1..749
                     /locus_tag="PZ377_002660"
                     /pseudo
     CDS             1..749
                     /locus_tag="PZ377_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            746..2070
                     /locus_tag="PZ377_002661"
                     /pseudo
     CDS             746..2070
                     /locus_tag="PZ377_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2070..2396
                     /locus_tag="PZ377_002662"
     CDS             2070..2396
                     /locus_tag="PZ377_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359513.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            2399..2686
                     /locus_tag="PZ377_002663"
     CDS             2399..2686
                     /locus_tag="PZ377_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359514.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            3003..3110
                     /locus_tag="PZ377_002664"
                     /pseudo
     CDS             3003..3110
                     /locus_tag="PZ377_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
BASE COUNT         1304 a          412 c          576 g          902 t
ORIGIN      
        1 ttggtattac tcattatatt gattggaggt tgtgtcatta tgacaaaaac aaatggtcga
       61 aacgctcaaa ttaaagaaaa tttcaacaaa acattaagtg tatatccaac caagaatctc
      121 gatgattttt acgataagga aggttttcga gatcaagaat ttgataaaag agataaaggg
      181 acttggatta tttattctga aatggttatc gaaccaaaag ggaaaaatat ggaatcgaga
      241 ggaatggtgc tctatatcaa tcgcaatact agaacaacga agggtaattt tattgtcacc
      301 gaaataactg aagatagtaa aggatattca cgtagtaaag aaaaaaatat cctgtcaaga
      361 tggaaaataa tcgaattatt ccaacaaagc ctataccgga tgacaagtta aaaaaagaga
      421 ttgaaaactt taagttcttt gtacaatatg gaaattttaa agattttaaa gattataaaa
      481 atggtgatat ttcatataat cctaatgtgc caagttattc tgcaaagtat caattgaata
      541 atgatgacta taatgttcaa cagttaagaa aacgatatca tattccaacc aaacaagcgc
      601 ccgaattaaa attgaaagga tccggcaatt taaaaggctc atccgtagga tctaaggatc
      661 tagaatttac gtttgtagaa aatcaagaag agaatatcta tttttcagat tcggtcgaat
      721 ttacacctag cgaggatgat aaatcatgag tcaaacggaa tatcaaataa aatctggcaa
      781 tataaaaggt aactctgaag aaacaagtac agtatctaat ataagttatg aaatagaaaa
      841 cgcaaataac agtggtttaa aacaaaataa aattgataaa caaattaaaa agttacaaga
      901 aaaaaataaa ttccctaaaa atctttcata tcttaaaagt tatacggacc ccaaaacagg
      961 cacgactaca agcgcctttt taaataaaga cactggcaaa gttactttag gtatgacagg
     1021 tactaatgta cacaaagacg caatattaaa acaaacattt ggtgttcctt cttatcaagg
     1081 atatatagat gtgagtgaaa cgctaaagga tattggggcc gatgtcaata ttggccttca
     1141 ttccgtcaca gataaagatc cacattataa aaatacccaa gactttatca aaaatatcaa
     1201 aaaagactat gatattgata ttattaccgg acattcgctg ggcggtagag atgcgatgat
     1261 tttaggtatg agtaatgata ttaaacatat cgttgtgtat aatccagctc cattagcgat
     1321 caaagatgtg agcggccttt atgccgatca agaagaactt aaaaaattga ttgaaaagta
     1381 cgatggtcac attgtaagat ttgtgtctga tgaagacgaa ttagatgcag gtgtccgcaa
     1441 tcatttatat gaaactgctg gagaaaaaat agtacttaaa aatggagaag gccatgcaat
     1501 gagtggtatt ttaatgagca gaacacaggc tataatctta gctgaattaa acaaagttaa
     1561 aggctaccaa gacgaaaata ataaagcatt aaaatccgtt cgtaaacaaa cgaggcatag
     1621 attacataaa gtagagacgt taagagcgaa ttggattcaa acaacgggtg gatcactctc
     1681 ttcctcccaa caacaattat tagaagcttt aacagcacta accattgccg aaggcttaaa
     1741 tcaattagtg aatgaagaaa gccaacattt gaaaaaatgt atcacgcgat ggcacataaa
     1801 tttggagaca actggaaaaa agcgcaagaa gttggaaatg aaattggtga aaaattaacc
     1861 tctgaagagg ttatagatga attaagaaaa ggtggcgcgt atgaaagtaa acttgaaaca
     1921 gatcccaaaa gaaaaattga tgataagata aagaaattaa atgatgttta taaaaattgt
     1981 aatggctata tcgcaaaaat taaacagagt atcgaagcaa ttgtttctaa tgaccaaatg
     2041 ttagcgagcc agattgatgg gatgatgtaa tgtttactac gtataaaaat attaatgaac
     2101 ttgaaaatgc ctatgatgaa gaaagaaaac aattgaatga tgcattcaat caaattgatg
     2161 aattaagaca tcaaacacgc aagaaatgtg aacaaatgta tgatcatttc ttatatctca
     2221 aacataaaat gaattatagt gaagacgcta tgatcaggat gacacgtatt atagaatctt
     2281 tcgatagaga aacgaatcaa cgtatccgac atcacgaaat gaaattagaa gattataaag
     2341 atgagttaag aagagaatat ctaaaacaat ctgacagaat tgaaggagat gaataagcgt
     2401 gggacttgat tttagtggtt taccagattt agcagtattg gaacaaatga aggaaaaaga
     2461 acagattagt gaggttattg cgcctgaaca tgttcgtatg catcatgatc atcaaaataa
     2521 gctgaaaagt gatgagaaaa tattacttga ccaaatggta agtcatttca aaaaatttga
     2581 agatgatttt aaaaatgcgg cacaaggggc ttgggtgaaa aatgccacag acgaattaaa
     2641 agatattagt aatgatttag aaaaaattca agatattaaa gtataaaaag gtattaagaa
     2701 acacaataag tatataatcc atttaataat aaatgaatta tatagttcat aatttcgact
     2761 ataagtggct attagccatt atttttcggg atctatgtca aatcggacta atgaattcaa
     2821 taatggaagt taagcaacca atctttgttt aacttctttt ttattttttg gaaaataaag
     2881 ttttgaacat aataaaattt gattatgttt taacgaattt tgatgtttct tgaactatat
     2941 atcatctagt cgtcatttac aattggtaaa tatgacttca aactgtatga aggtgatggc
     3001 gattaaaagg ctcatccgta ggttctaaag aactagaatt taactttata agaaataaag
     3061 aagaaaatgt gtatttttct gatagtatta atttcaaacc tactgaatag ggttaataga
     3121 agtttgaaat aaaaatttca taaagtgttc aaaataattt ttcgctttag ttgtaaatat
     3181 taaaaactga gtca
//
LOCUS       DANRDI010000223         3156 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.225, whole genome shotgun sequence.
ACCESSION   DANRDI010000223 DANRDI010000000
VERSION     DANRDI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.225"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..148)
                     /locus_tag="PZ377_002665"
     CDS             complement(<1..148)
                     /locus_tag="PZ377_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359515.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEM"
     gene            complement(384..2480)
                     /gene="pnp"
                     /locus_tag="PZ377_002666"
     CDS             complement(384..2480)
                     /gene="pnp"
                     /locus_tag="PZ377_002666"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDM6359516.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            complement(2850..3119)
                     /gene="rpsO"
                     /locus_tag="PZ377_002667"
     CDS             complement(2850..3119)
                     /gene="rpsO"
                     /locus_tag="PZ377_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDM6359517.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT          954 a          632 c          447 g         1123 t
ORIGIN      
        1 gcatttcgtc ttctggaaac ataagtccag catctaacat aaacatttca tcgtctactt
       61 caacgatata catattttta gcaatttcgc caacaccgcc taatggtata atgcgaatat
      121 ctttattctt tttctttatt aaactcaaaa tcttacctcc taaatttgtt acccgtccat
      181 atataaactc actattcatt ataagttaaa aaacacttga tgtacactat taatcactat
      241 acatacagga taattaacag ttgcacacaa aataataaag cgttttaaaa tgttcaatac
      301 aacaaattca aaacgggtca cttcgtaaaa agtggcataa aaaaatcaca tgacgataca
      361 ttgtcgtcat atgactttaa atattattct tctaatgctc tatgtgaagc atttactcga
      421 ccttgtttat caatttcagt aatcttaact tcaattgtgt caccgatttt taatacatct
      481 tccacttttt caattctatt ttttgaaatt tgtgaaatgt gaagcaacgc atctttacct
      541 gggaataggc ctacaaacgc accgtatttt tcaatacgtt taacagtggc ttgataagtt
      601 tgacctactt ccgcttcacg tgtaatttcc tcaatgattt cacgagcacg atttatcata
      661 gcttgatcaa cagcaccaat aaagatagta ccatcttgtt caatatctaa tttaacacct
      721 gtttcatcaa taatttcgtt aattttttta ccaccaggtc cgataacatc tctaatctta
      781 tctggtttaa ttgtcatagt tacaactttt ggcgcgtaag cacttaattc agtacgtggt
      841 tgatcaattg tttgtaacat atgattcatt atttctaaac gaccacgtct cgcttgttct
      901 agagcctctt cgataatttc acgcgttaaa ccgtcaattt taatatccat ttggattgct
      961 gtaatacctt ctttagtacc agcgacttta aagtccatat cacctaatgc atcttccata
     1021 ccttggatat cagttaaaat cgtatagcta tcttcacgtg taacaaggcc catagcaata
     1081 ccagcaactg gtgctttaat cggtacgccc gcatccatta atgctaatgt tgatccacaa
     1141 attgacgctt gagatgatga accatttgat tcaagtacct cacttacaat acgaattgta
     1201 tatgggaaat cagcagtatc aggaataata tattttaatg ctctttcacc taacgcacca
     1261 tgtccaattt cacgacgacc tggcgcacgt actggaccag tttcacctac tgaaaaattc
     1321 gggaagttgt aatgatgcat gaatcttttt tcttcttcag gtcctaaacc atcaattaat
     1381 tgataatcgc ctaaagcacc taatgttaaa actgaaagtg cttgagtctg accacgtgta
     1441 aatagacctg aaccatgcgt tctaggtaaa ataccaactt cagaatctaa tggacggatt
     1501 tcatcaggtt tacggccgtc tggtctaatt ttttcatctg caattaaacg tcgaacttct
     1561 tctttcacta attcatttaa aattgcataa acttctttaa taagtaattc attctctgga
     1621 tcttcttcat cgataaattc attgacgatt tcttctttta agttatcaag attttcatct
     1681 cgttgttgtt tatcaaatgt taaaactgtt tctttaagtc ctttttcttc ggttaaagat
     1741 tttacacgtt caactagcgc ttcatcacgc tctgctggaa taaattcttg tttaacaggt
     1801 tgaatgtggt cgacgatttg ttgttggaaa tcaactaaac gttgaatctc ttcatgacca
     1861 aagaaaatcg cctctaacat ttcttgttca gtaatctcac tagcgcctgc ctctaccatg
     1921 tttaccgcat ctttatgacc agctacttca aggtctaaac gagaaacttc tttttcttct
     1981 actgttgggt taatgatata tttaccgtca atataaccca catttacacc ggcgattggc
     2041 ccttggaatg gaatatccga cacactaagc gccatagatg aaccaatcat tgcagccatt
     2101 tgtggtgaac aatcaggatc tgcacttaat accatgttca taatttgaac atcatgctta
     2161 tatcctttag ggaataaagg tctaattggt ctatcaatta atcgcgcagt taatgttgca
     2221 tcgtcaccag gacgtccttc tctcttttta aatccaccag gaattttacc cgcagcgtac
     2281 attttttctt catagttcac tgttaatggg aagaaatctc catcacgagg ttcttttgat
     2341 gcagttgccg tcgataacac gactgtatct ccataacgaa ccaatacagc gccatttgct
     2401 tgtttagcta attgccctgt ttcaatcgtt aaagatcttc ctgcccattc agttttaaaa
     2461 actttctttt cttgagacat tataaatctc ctctcttgaa tctaatagtt atatcatatc
     2521 atttattatc gtattttaat attagttata aaatattgaa ttggaaaaaa agagagcggg
     2581 atatccatct attatttaca aaatagactt atcgtcccac ccttatcagt ataaatataa
     2641 gttatatttt ttaaaaaaac atactttatt tttaatatct tgattattac tctaggtgta
     2701 cttatatcat ctttatctca ttgtaacctt attattcact caatattagc ttaacatata
     2761 ataaatgtta atatttgtaa aaagcttttt taatataaat taaggttgga acataaatat
     2821 gccccaacct caaagacgtt atattaagat taacgacgga taccaagtga tttaattaat
     2881 tcacggtaac gttgaatatc tttactacgt aagtagttta ataaatgtct acgacgacct
     2941 accattttta ataatccacg acgtgaatgg tggtcttttt tgtgtgtacg taagtgttcg
     3001 tttactgcgt tgatttctgc agtaagtaca gcgatttgta cttctggtga accagtatca
     3061 gtttcgtgta cacggtattc tttaatgatt tcgtttttac gttcttgtga aattgccata
     3121 atcaatttcc tccttaaaat gtttgaatac tcctta
//
LOCUS       DANRDI010000224         3126 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.227, whole genome shotgun sequence.
ACCESSION   DANRDI010000224 DANRDI010000000
VERSION     DANRDI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.227"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..942
                     /gene="xseA"
                     /locus_tag="PZ377_002668"
     CDS             <1..942
                     /gene="xseA"
                     /locus_tag="PZ377_002668"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDM6359518.1"
                     /translation="PKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGE
                     KAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGH
                     ETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHV
                     EHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALL
                     SSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKK
                     DEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            935..1165
                     /locus_tag="PZ377_002669"
     CDS             935..1165
                     /locus_tag="PZ377_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDM6359519.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            1143..2024
                     /locus_tag="PZ377_002670"
     CDS             1143..2024
                     /locus_tag="PZ377_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDM6359520.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            2456..2908
                     /gene="argR"
                     /locus_tag="PZ377_002671"
     CDS             2456..2908
                     /gene="argR"
                     /locus_tag="PZ377_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDM6359521.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            2924..>3126
                     /locus_tag="PZ377_002672"
     CDS             2924..>3126
                     /locus_tag="PZ377_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDM6359522.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGI"
BASE COUNT         1202 a          413 c          562 g          949 t
ORIGIN      
        1 cctaagtttc ctaaaaaaat tgcagtttta acagcgagta caggtgccgc aattcgagat
       61 atccattcaa cgataaatag tcgttttcca ttagctgaac aaatacaaat tagtactttg
      121 gttcaaggtg aaaaagcaaa agacgacatt attgaaaaaa ttgaatatgc agatagttta
      181 ggtgtagata ccattattgt aggtcgaggc ggtggatcca tagaagattt atggaatttc
      241 aacgaagaag ctgtcgtccg tgcaatatat aattgtaaga caccgattat atcagcagtt
      301 ggtcatgaaa cagactttac attaagtgat tttgctgcag acatcagagc tgcgactcca
      361 actcaagctg ctgttattgc aacaccagac caatatgaat tgctgcaaca aatacagcaa
      421 tatcagttta cattgactcg tttcatcaag aaacatttag aacaacaacg taaacatgtt
      481 gaacatttgt catcgtatta caagtttaaa caaccaactt tattgtatga tcaacagata
      541 caacgtcgag atgatttaga aaagagactg aaacaacaaa ttcaggcaac ttttgaacaa
      601 cagagacatc gattaatgct gttgcaacaa cgttataatt taaaagcatt actaagtagt
      661 gttaatcaag aacaacaaaa taatcttcaa ttgacaaacc aacttgttaa attattgaat
      721 tctaagatat taagttataa aaatgatttg aaaaataaag tagaaaactt aaacaattta
      781 agtccaacta atacaatgtt gcgtggatat gcaattgtta ataaaaaaga cgaagtcatt
      841 acgagtacta aagatttaac agaaaatgat caattgacat taacaatgaa agatggctta
      901 gtagatgcaa aagttacgaa agtaaggtgt aataatgact aaagaaacgc aaagttttga
      961 agaaatgatg caagaattag agcaaattgt tcaaaaatta gataatgaaa cagtatcttt
     1021 agaggaatca ttagatttat atcaacgtgg tatgaaacta tcagcagctt gtgacacaac
     1081 tttaaaaaat gccgaaaaaa aggtgaatga cttaataaaa gaagaagctg aggatgtaaa
     1141 aaatgacgaa tctaccgatg aataaattaa tagatgaagt caataatgaa ttatcggttg
     1201 cgataaataa atcagtaatg gatactcagc tagaagaaag tatgttgtat tcattaaatg
     1261 ctggaggtaa acgcatccga ccagttctgt tattactcac tttagattca ctaaataccg
     1321 agtatgagtt aggtatgaag agcgcaattg cactagaaat gattcataca tattcactta
     1381 ttcatgatga cctaccagcg atggataatg atgattatcg acgaggaaaa ttaacaaatc
     1441 ataaagtata tggtgagtgg actgcgatat tagcaggtga tgctttatta actaaagcat
     1501 ttgaacttat ttcaagtgat gatagattaa ctgatgaagt aaaaataaaa gttctacaac
     1561 ggctgtcaat agcaagtggt catgttggaa tggtcggcgg tcaaatgtta gatatgcaaa
     1621 gcgaaggcca accaattgat cttgaaactt tggaaatgat acacaaaaca aaaacaggag
     1681 cattattaac ttttgcggtt atgagtgcag cagatatcgc taatgtcgat gatacaacta
     1741 aagaacattt agaaagttat agttatcatt taggtatgat gttccagatt aaagatgatt
     1801 tattagactg ctatggtgat gaagcaaagt taggtaaaaa agtgggcagc gatcttgaaa
     1861 ataataaaag tacgtacgtg agtttattag ggaaagatgg cgcagaagat aaattgactt
     1921 atcatagaga cgcagcagtg gatgaactaa cgcaaattga tgaacaattc aatacaaaac
     1981 acttattaga aatcgttgat ttattttata gcagggatca ctaaaagcaa atatatcgat
     2041 tagcaacaat tgtaaagttt gaaatgtctc aaaaggcata ttcaactgac gttgtttgtt
     2101 aatcgatttt tctcatgttg aaacttaaaa ttatgttgaa gactttcatt taaaaaatag
     2161 aattttcaat ttctatacga caatattcgt atctgtgata tcaatgtgat tttaattaaa
     2221 tatgttcaaa caattgacgc ttattaaatc taataattag caagtttatt acacatatat
     2281 tcatttttat acatatttta agcaattgtt atgcgtaata tgaaaaatat ttataaatag
     2341 ataaccaaga tagataaacc atgtttaccc tttttacgta aaggtgttta gtagtcgtaa
     2401 atcagtgcat gtgttaaaat aaatgtataa atattcgtaa tcagaggtgt ttactgtgcc
     2461 caaaaaatcg gttaggcata taaaaattag agaaattatt tcaaatgaac agatagagac
     2521 acaagatgaa ttagttaaac gattaaacga ttatgattta aatgtcactc aagcaactgt
     2581 ttctcgtgat attaaagaac tacaacttat taaagtacct ataccttcag gtcaatatgt
     2641 ttatagttta ccaaatgata gaaaattcca tcctttagaa aaattgggac gttatttaat
     2701 ggattccttt gttaatatag atggtactga taatttactt gttctaaaaa cattacctgg
     2761 taatgcacaa tctattggag ctatattaga ccaaatcaat tgggaagaag tactaggcac
     2821 aatttgtggt gatgatactt gtttaattat ttgtcgaagc aaagaggcaa gtgatgaaat
     2881 caagtcaaga attttcaatt tgttataagg atgcgatgag tttatgttac aaaccttatc
     2941 aatcaagcaa tttgctatta ttgaagaatt agaaattcaa ttttctgatg gtttaactgt
     3001 tttaagtggt gaaacgggtt caggtaaatc aattattata gatgcaattg gacaattaat
     3061 tggaatgaga gcttcatctg attttgtaag acatggtgaa aaaaaggctg tcatcgaagg
     3121 tatatt
//
LOCUS       DANRDI010000225         3114 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.228, whole genome shotgun sequence.
ACCESSION   DANRDI010000225 DANRDI010000000
VERSION     DANRDI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.228"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1300
                     /locus_tag="PZ377_002673"
     CDS             <1..1300
                     /locus_tag="PZ377_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDM6359523.1"
                     /translation="FQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERF
                     LMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNP
                     EGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDR
                     NVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVST
                     EQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQI
                     ELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYL
                     SLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDEDALFDKV
                     KYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(1424..2896)
                     /locus_tag="PZ377_002674"
     CDS             complement(1424..2896)
                     /locus_tag="PZ377_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDM6359524.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(3015..>3114)
                     /locus_tag="PZ377_002675"
     CDS             complement(3015..>3114)
                     /locus_tag="PZ377_002675"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDM6359525.1"
                     /translation="ENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1018 a          492 c          457 g         1147 t
ORIGIN      
        1 cttccaagaa gcaacatatt tagaacgcaa tgaaccattt gactggttgt cgtattggac
       61 tgatgaatgt cattatactt taaaacatgt tgaaaatagt catgacttta gaatatatga
      121 tcaagagcgt ttcttaatgt atgcccattt tcaagatcct aaatatcgta tattagatta
      181 tgtaaatcat tttgatagtc aaagacgaaa agtaaaacgt gatttttatg atgtgcgtgg
      241 ctttttaagt tgtagtagga ttcttgttga taaacaacaa acattatgtg aatttttcta
      301 taatccagaa ggtgacacaa aattagaaaa gtatttttct tacaaggatg gcaaaccaga
      361 agtacaaaaa attattgttt actatgctaa caaacaatat ttctttaata atgaaacgga
      421 attaggcgca tttttcatta aacaactata tcaacatggg gatttatttt tcagtgatcg
      481 taatgtgtac acagcaccta ttttcaactt aacgcctgag tcaatcccag tagtagctgt
      541 gcttcacagt acacacatta aaaatattga tgcattagat tcatcgccat ttaaaaatgt
      601 atataaagcg atgtttgaaa atttatctcg atatcgtgca attatcgttt ctactgagca
      661 gcaaaaatta gatgttgaaa aacgtattaa ccatactatt ccagttgtta atattcctgt
      721 cggctatagt gaaacaattg atacaccagt ccaaacatta gatcagcgca gtgttaaatt
      781 aatttccgtt gctcgttatt cacctgaaaa acaattacat caacaaattg aattaattaa
      841 acgcttagta tcgtatgtcc caaaaataga gcttcatatg tatggttttg gttctgaaag
      901 taaaaaatta aatgaattga tacaaaaata tggattggaa aatcatgttt acttacgcgg
      961 ctttttaagc aatctagacc aagaatatag cgatgcctat ttgagtttaa taacaagtaa
     1021 catggaaggt ttctctttag cgctacttga atctttagca catggtgttc cagttattag
     1081 ttatgatatt aaatatggac ctaacgaact gattacttca gatttcaatg gttatttaat
     1141 tactaaaaat gatgaagatg ccctatttga taaagtaaaa tatgtgattg atcatcctga
     1201 agtacagcaa cgactgtcaa aaggtagttt ggcaaaagca caacaatata gtaaagcgtc
     1261 gttaattaaa caatgggacc aatttgttcg tcttatataa atgtacttta cctttatttt
     1321 tacaaaaata gcattttcct atgtcattta actaaacatg taagttcgta tgaacgaggt
     1381 ttgttaaata gatgattcta ggaaaatgct tttttctttt gacttagttt aaaatatttt
     1441 gccactttgt actgatagta gttgcattgt actgttgtgc agattctatg ctattaattg
     1501 aaaactgttg caatttttga gtattattta gtaattggtc gaccttttca accatttgat
     1561 tgatgtcacc ttgaggtact aaatagccat taaatccatc ttgaatcagt tctgatggac
     1621 cataatctac atcataactg atcactggtg tacctactga aagcgactct aaaattgcta
     1681 agccaaaacc ttccatttta cttgtcgata acatcagttc tgctttagca atctcttcat
     1741 taatatgcgt cttaaaacca tgaaatttaa catgttccga taaatgataa tcttctacaa
     1801 gttgtcgata ttctgacaaa ccatttccat gtccataaat attcaattga atattgggat
     1861 gttttgttac taattgcttg attacttcaa tttgatgttt aatttgttta ttttcaacga
     1921 ggcgagcaat tgatatgata tgatttttct ccttttgatt gatgtcaaat tgatacttta
     1981 aatttgccac gtagccaacc ggaatattga taactggtat tttattttca atatattgtg
     2041 aaatatcttg gcattgcttt tctgttgata caacaatcgc tttataacgt gttaaattat
     2101 taaacactgt tttataaaaa ctttttatac cattaccggc accggataaa tgtgtactat
     2161 ggagtacaac aataactgga atactttgat ttaatcccgc tataacattt cctaattcat
     2221 gaggacgatc taatatgatt tgatcattat ttttacataa ttgatggaga aaatattgaa
     2281 ctaattcatc ttctgtatca aaaaattgtt gatgctggtc ttcatttaag ataacctttg
     2341 tgagcgtgtt tttccctttt atatcgtcaa aatatttttg gatgacgatt tccccattag
     2401 gtgtatagta attttcgagt acaatccgtt gtccttcacc taaaattcga gaacaactta
     2461 aaaagcctct tccatcatac aattcgcgtt ttacttttct tcttttatga tcaaaataat
     2521 tcacataatt taattgatga tactgtttat ctaaaaaatg agcatacatt acaaattgct
     2581 cttcatcata tattctgaca tcatttgaat tttccacaaa tttcaatgtg tacctacatg
     2641 acttttccca atactgtatc cagttaactt gctttgtctt tttataattg attgcttttt
     2701 gaaaatagtc atacattgta aatacatcat tttcaatctg atgttgcttc gcatatgtgt
     2761 atgaataagg attccattta acatatacac attttgaaga tatgccgtgt tgtttgaaca
     2821 acttcaatct atttatttgc gctttttcta cacctgtaat tttactttct aaaattgttc
     2881 ctaaaatgta attcatatta tcgcctcata taagttttat tccgtatctt tattgtttat
     2941 tttatatgaa aaatacatct attgcatgtg taattataaa aaaaccaggc cacaaggacc
     3001 tgggtcatat tgtattattt gttttgtttt ttgcgacgac cgaataacaa taatgaacct
     3061 aatgctgcaa ataatccacc aaataacgtt gcgttatttg agccgttatt ttca
//
LOCUS       DANRDI010000226         3104 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.229, whole genome shotgun sequence.
ACCESSION   DANRDI010000226 DANRDI010000000
VERSION     DANRDI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.229"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1262
                     /locus_tag="PZ377_002676"
     CDS             <1..1262
                     /locus_tag="PZ377_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359526.1"
                     /translation="VTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEV
                     IKTFNQSQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLI
                     SINQLNYAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTK
                     FKKPQHYEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLIS
                     RYWDVTSGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEA
                     VEKAAKLAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEA
                     TVYVDPDNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLL
                     LKLNGNYKKMWDTHMHTKDWGINTGHN"
     gene            1287..3020
                     /locus_tag="PZ377_002677"
     CDS             1287..3020
                     /locus_tag="PZ377_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDM6359527.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIHDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
BASE COUNT         1175 a          397 c          556 g          976 t
ORIGIN      
        1 tagtaacgat accaatttca attttcgctt ttaaaaaagt tatgtctgga tttaatgaga
       61 catatgctga acaaatgaaa tcgaataatt atatgaatag cgcgattgta gagtttatcg
      121 aaggcattga agtgattaaa acatttaatc aatctcaaag ttcatataaa aaatataaag
      181 atgcagtaga taattataag attcacactt tgaattggtt taaaaataca tgggggtata
      241 tgaatttagg agctagtgta ttaccttcaa catttttagg gattttaccg gtcggcatgt
      301 atttgatatc tatcaaccaa ttaaactatg cggaattttt cctatgcatc gtattatctt
      361 taggtgtagt agcaccgatt aagaatttta ctaattatgt aaatcattta aagtctatac
      421 aatacgcatt aactgaagtg aatcaaatat taagtctaga agagttagta ttgtcaacaa
      481 aatttaagaa gcctcaacat tatgaaattg cttttaataa tgttggattt tcatataaca
      541 aagataagga cgatctggtc tttaagcatt tatcatttac agtaccagaa aataatttca
      601 cagctatcgt cggggcatct ggtagcggta agtcaaccat tgccaagctt atatcacgat
      661 actgggatgt gacttcaggt gaaattacga ttggcggtat aaatataaaa gatattgaat
      721 cgaaacaact taacgattta gttggttttg ttggacaaga taacttttta ttaaatctta
      781 cttttaaaga aaatattaaa cttggcaatc cagaagctac ggatgaggca gttgaaaaag
      841 ctgccaagtt agcacaatgt catgaattta ttgaaaagtt gccagatgga tatgatacga
      901 atgttggtac agtgggagat aaattgtctg gtggtgaaaa acaacgagtc actattgcaa
      961 gaatgatatt aaaagatgcg cctatcattg tattagatga agcgactgtt tacgttgatc
     1021 cagacaacga acaaaaaatt caagaagcat taaatgtgtt gacgcaggat aaaacattga
     1081 ttgttattgc acatcggtta tctacgattc aacatgcaga tcaaattatt gttttaggta
     1141 aacaacaaat tttagaaaaa gggtcacatc acttattgct aaaattaaac ggtaattata
     1201 agaaaatgtg ggatacgcac atgcatacaa aagattgggg aatcaatact ggacataatt
     1261 aaactaatga gaaaaggagg tcaaatatgt ttcaaattac atttaaaatt ttaaactgga
     1321 taagaccata taaagcaaga atgattttag gatttagcat gtcattttta aatgctattt
     1381 ttattgcgtt acctatcttt ttagctgcaa aaatatttaa taatgtgcta tctcataaac
     1441 ctatttatat gaaagatata ttgaatgttg taattatcat ggttttatta gtgattggac
     1501 gatttattac agcatatttc aaaagcaaaa gccatgagag catcgcttat gaaatgagtg
     1561 cgaaagaacg tttagatata ggggataaat tgaaaaatgt aaggttaggt tattttaatt
     1621 cgcatcattc aaatgagtta acaacaatag taacaacaga tttaaccttt ttagaaaact
     1681 ttgctatgaa aatggtggac gttgttgtta atggatacat attaattaca gtactcatat
     1741 tgtctctact tgtagtttcg tggcaagtat cattattagc atgcattggc gtattactat
     1801 cattttttgc gattcaatta ttagaaagaa agagtcgaca aaatgcgcca gcgtatcata
     1861 atgtacaaaa ccaattagtg gaaaaggtat tggaggttat tcgtggtatt caagtaataa
     1921 aatcattcgc gaaagaaaat acgagtctta aaagttttaa ccaagcagtc aatgaaagta
     1981 aacgtataaa tacaaaaata gaaatgcaat atatcccatt caatttattg catttactta
     2041 gtttaaaagt tgtttcaata atgattgtat tagttgcatg cttattatat atgaatcata
     2101 gtattgattt acctaccctt attatgattt caattttttc atttgtgata tttgatagtg
     2161 ttgaaaatat taatagtgct gcacacgtac ttgaaatgat agatatgacg atagatgata
     2221 ttgaaaagat aaaaaatgct ccagaactgg atgagaatgg aaaaaatttg acgattaaaa
     2281 atgaaaatat cgcttttcaa aacgtgaact tttcatatga tgataaacaa gtgataaaga
     2341 atgtgaattt tgagatacct acacaaacat caacagcaat aattggacct tcaggaagtg
     2401 ggaaatctac attatgtcac ttactcttgc gcttttatga tatccatgat ggaaatattc
     2461 gcatcgatgg tgttgatatt aaagatatga cattaagtac gttaatgtcg aaaattagtg
     2521 cagtatttca aaaggtgtat ttatttaatg atacgattga aaataacata ttgtttggca
     2581 atccaggtgc aacgaaagaa gaaattattc gtgcagcgaa gcaagcatgt tgccacgact
     2641 tcatcatgtc attacctgag ggatatcaaa caatgctaaa tgaaaaaggt agtaatttat
     2701 ctggcggaga aaagcaaagg atttctattg ctagggcgat attaaaagat gcaccaataa
     2761 ttattttaga tgaagcaact gcaagtattg accctgaaaa tgaacagctg attcaaacgg
     2821 caattaatga attaagtaaa ggcaaaacag taattacaat tgcacataaa cttgaaacta
     2881 ttaaaaatgc agatcagatt atagtgctca atgaaggtga aataattcaa aaaggtagtc
     2941 atgatgaatt aattcgaaaa ccaggaatgt atcaagactt tataagaata aaaagtaaat
     3001 cagcaggctg gaaattataa ataatagttt aatatggaat cagaatttgg aaataaaaag
     3061 ggtgatagat aatcaacagc aaaataaatc ccctcactag ggca
//
LOCUS       DANRDI010000227         3060 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.230, whole genome shotgun sequence.
ACCESSION   DANRDI010000227 DANRDI010000000
VERSION     DANRDI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.230"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            140..775
                     /locus_tag="PZ377_002678"
                     /pseudo
     CDS             140..775
                     /locus_tag="PZ377_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1218
                     /locus_tag="PZ377_002679"
     CDS             772..1218
                     /locus_tag="PZ377_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6359528.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDDKCVGKVKLRKNWNRYAYIEDIAVCKDFRGQGIGSALINISIEWAKHKNL
                     HGLMLETQDNNLIACKFYHNCGFKIGSVDTMLYANFEKAVFWYLRF"
     gene            1311..2105
                     /locus_tag="PZ377_002680"
                     /note="aph(3')-IIIa"
     CDS             1311..2105
                     /locus_tag="PZ377_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDM6359529.1"
                     /translation="MAKMRISPELKKLIEKYHCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2381..2952
                     /locus_tag="PZ377_002681"
                     /pseudo
     CDS             2381..2952
                     /locus_tag="PZ377_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1023 a          485 c          698 g          854 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa
      961 aattggaacc ggtacgctta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     1021 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     1081 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     1141 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa agctgttttc
     1201 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     1261 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     1321 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccactgcgta aaagatacgg
     1381 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     1441 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     1501 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     1561 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     1621 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     1681 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     1741 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     1801 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     1861 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     1921 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     1981 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     2041 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     2101 tttagtacct agatttagat gtctaaaaag ctttaactac aagcttttta gacatctaat
     2161 cttttctgaa gtacatccgc aactgtccat actctgatgt tttatatctt ttctaaaagt
     2221 tcgctagata gagttctata ttagagatgt aaagagtttt ggtgaagagg tttgtgagta
     2281 tggcttttat aaccagggtg caatgagaag cactaacagc agctagctta agaacgcata
     2341 ggatatactt tttatggaag tcacagagga ggaataaaat ttgaacagga ttaacagagt
     2401 aacctctatt cttattcagc tgcaatcaaa aaaataattc ctgccaaaga aattgcacag
     2461 cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga
     2521 ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttctg
     2581 ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat
     2641 tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct
     2701 attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac
     2761 agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca
     2821 atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa
     2881 agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta cttaatcggt
     2941 tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa agcatttgca
     3001 tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta aaacaaatgc
//
LOCUS       DANRDI010000228         3041 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.231, whole genome shotgun sequence.
ACCESSION   DANRDI010000228 DANRDI010000000
VERSION     DANRDI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.231"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            110..565
                     /locus_tag="PZ377_002682"
     CDS             110..565
                     /locus_tag="PZ377_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HDM6359530.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(733..2310)
                     /locus_tag="PZ377_002683"
     CDS             complement(733..2310)
                     /locus_tag="PZ377_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDM6359531.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            2505..>3041
                     /locus_tag="PZ377_002684"
     CDS             2505..>3041
                     /locus_tag="PZ377_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359532.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQEL"
BASE COUNT         1032 a          560 c          420 g         1029 t
ORIGIN      
        1 actcatatct tcaaaaaaaa tgacaaaatt taaatttttg aataacatca ttttttagta
       61 aatgttataa tttctttata atacaaatat cgaaagaagg cgactgactt tgaaaaaatt
      121 atgttcatta attgtagtag cattagtttg tatcattgca ttatcagctt gtggtaaaga
      181 acaaaccaaa acatatgaag gtgatgttag tggtaaacat gtactaactt ctatcacata
      241 taaagatgat aaagttttaa aacagtctac aatcaacaca attaaatatg acgatctagg
      301 tatggataaa gacgaagcca aaaagttatt cgccaaatct gaaagtattt tcaaagacct
      361 taaaggcgta aaatacaaag tagactataa agataaaaaa gcaattgaac acttagacat
      421 agattacaca gaagttgaca tgaaaaaatt aaataaacgt cttggtgttt cgactaaaga
      481 aaataaagat attagttttg aaaaacttga aaagcaatta aagcacagag gtttaaaaga
      541 aaaagataaa atggacgaca aatagtttat aacttaaaaa tgccctcaga taagactaag
      601 gttacaaacc ttaattcata ttctgagggc tttaatattt gaagttcttg tgtgaccagc
      661 atccactact aatataaaat tatttgcagt aacgctaaaa tccgctgctt tcaatttccc
      721 gaaataatta agttaactaa tgagttttaa tttataatca tgtatcgttt gtaactcacc
      781 atcgactttt cgatatacaa tatgatcagc agtaatttct gtaggactgg atacgccaac
      841 agctgctgca atattgaata agccttcatg caaacttgtt acatagtttg tgacacgata
      901 ttgcttttct ccaacaatca atgctttttc tttcttcgca tctgtcgttg caacacctac
      961 aggacacgta ttcatgtgac attgttgact cattatacaa ccgacactaa tcatcatccc
     1021 acgtgcgata tttacaaaat ctgcacctaa acctagtgca atcgcaattt tatctggtgt
     1081 cactaactta ccagatgccg ccaatttcac tttatctcga ataccatatt tttctaacat
     1141 gccagacaca ataggtagag ctgtaaatag cggtaagcca acaccatctt gtaattcttg
     1201 gaatgttgca ccagtaccac cttcaccacc atcaatcgta ataaagcttg gatacttatc
     1261 tagttccacc atcgtacgta caagtgtttc aatttctgaa actttgctta ctacaatttt
     1321 gaatcctact ggtttttgac ctaattgctg caactgatcg acgaaacgaa tcaaatcttc
     1381 agcattatga ataaattcgt aacggttagg tgaattgatt gttttataag gttcaacatt
     1441 tcggatttta gcaatttctt cgtttacctt ttcagcttcc atatgaccac cacgagtctt
     1501 agcaccttgt gccaacttca gctcaaatgc gcgtacgtta gataactgtg caacctcttt
     1561 aaataaacct tcactaaaat taccttcttt atcacgaaca ccaaataaac cgggaccaat
     1621 ttggaaaatg atatccccat taccttttaa atgatattct gataagccac cttcacctgt
     1681 attcatccaa gtgcccgctt tagctagacc tttagataaa gctgtaatgg cattttttcc
     1741 taaagcgcca taactcatac cagattgtcc tacgatacgt tttaaaataa atggatgttt
     1801 taaatgttca cctaatttta ttgcatggtc atcacttaag taatacggat caatctttgt
     1861 cggcacacga tattcttcac gactaaataa acgctcattc gcgattttat aaatgaatgt
     1921 tgataacaat gttgtattat ctactgaaat ctcattacgt tgcatcggaa acattgtgtt
     1981 ctgtatgtaa aagccgtctt gataatcttt agtagtaccg aagctggtca tacgagagtt
     2041 atattttcca gccaaaacga tatttttata atcattacgt gaaaaaggtt tcccttcatt
     2101 atccccagaa aataaatact gacgtaattc cggtcccatt ttttctgaaa tatatctaat
     2161 acgtgctagt aaaggataat tccttaatac actatgttgt gattgtcttt tatctttaat
     2221 taaccaaata agcccgataa caataaccgt aagcatgaat cctacaacga taatgttaac
     2281 tataaattgc atgactgtaa gaaacgtcat tacaatacct ccccccaaaa tttcaattca
     2341 atatttatga tacaccttac aaaacaaaac acaatggaag cgcttcattt tataaaacaa
     2401 ttttatgata tgtttttcat tttaaatttt aatgtataaa acatacaata caaagtaata
     2461 tgtgctaaag tatctatata atacaactat ttaagaggta tactatgtca aatacaaata
     2521 aacattacat agaagaagaa tacgctaccg aacaatcgcg ttttttcaaa cgtgatattg
     2581 gatttatttt cctttacata tttttggtta acatcttgcc gatcattatc gtctttttaa
     2641 cttttgggtt agtagctgta ttcactcaac aagctccgtg gggtttctct tttcctattt
     2701 caatgaacat tagctattta ctcgcagtta ttatttcact agctattttt cgtctgatgc
     2761 atgaagatgc gtttattcca atgatacgtt cgcaaatcgt tgctagtgct aaatataaat
     2821 ggcatattct tatatcattt acactaagtg tcgtactctt tttactttta aatcatttca
     2881 atattttcgc actaaatgta gattccttta ctgtctttga catttacccg ctcattaaag
     2941 ggtatggttc catcagttta ataactatat tttcgttaac cattatatta aatactttga
     3001 tttgtcaact tattttccgt catattttaa ttcaagaatt a
//
LOCUS       DANRDI010000229         3014 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.232, whole genome shotgun sequence.
ACCESSION   DANRDI010000229 DANRDI010000000
VERSION     DANRDI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.232"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..703
                     /locus_tag="PZ377_002685"
     CDS             <1..703
                     /locus_tag="PZ377_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDM6359533.1"
                     /translation="GDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGS
                     KNLIDAAKKHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEE
                     TARMDEYVVLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQ
                     AIHFENGIYNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGG
                     TLIYQTWKDGMNPIK"
     gene            complement(795..2291)
                     /locus_tag="PZ377_002686"
     CDS             complement(795..2291)
                     /locus_tag="PZ377_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDM6359534.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            2523..>3014
                     /locus_tag="PZ377_002687"
     CDS             2523..>3014
                     /locus_tag="PZ377_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6359535.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLT"
BASE COUNT          985 a          425 c          562 g         1042 t
ORIGIN      
        1 tggtgatata ttaaaagctg atactattga tcaagcgtta gcagatttta aaccagaaat
       61 cattatcaat caaattacgg atttaaaaaa tgttgatatg gcagcaaata cgaaagtacg
      121 tattgaaggt tctaaaaacc taattgatgc ggcgaaaaag catgacgtta agaaagtaat
      181 tgcccaaagt attgccttta tgtatgaacc tggcgaagga ttagcaaatg aggaaacttc
      241 acttgatttt aactcaactg gcgatagaaa agtaacggtt gatggtgtgg ttggtttaga
      301 agaagaaacg gctcgtatgg atgaatacgt tgttttacgt tttggctggt tatatggccc
      361 aggtacttgg tacggaaaag atggcatgat ttataatcaa tttatggatg gtcaagtgac
      421 actttcagat ggcgtaacat catttgtgca tcttgatgat gcagttgaaa catctattca
      481 agctattcat tttgaaaatg gtatctataa tgtagcagat gatgcacctg ttaaaggttc
      541 tgaatttgca gaatggtata aagaacaact tggtgttgaa ccaaatattg atattcaacc
      601 tgcgcaacca tttgaacgtg gcgtaagcaa tgagaagttt aaagcgcaag gtggtactct
      661 gatttatcaa acttggaaag atggcatgaa tccaattaaa taataattta tccgtttaat
      721 atacaaagaa taaagacttg gtcgaatcgt ggatgatata ttatcaaacg cacggctcga
      781 acaagtcttt tttattatgt cttcgttatc tttgtatgaa ggaataacag aattacaatt
      841 aatgtactga ataatgcaat taatgttgtg attagtgcta atttaatttc tattggtagc
      901 caagtcagta caaaagacca attattgcta ccgagaatga gatatggtaa tgcatataat
      961 atgagcgcta aagcgataca tatacataat gataaccaac tcaatacagc aatccgtttt
     1021 gatctacgca aaagaatttg accatggcgc atttgccaag cacgataagc tgagaatata
     1081 aatgctaata aaataagtgt tgtcatcaaa acggtaacaa tattaaattg atctttatat
     1141 tgattgagca tagacgcaac cgtcgaatat cgcttgtgat ttacaatttg tgtattaaga
     1201 tgctcaacta atttgggtac atattccgaa tttagatttg caagtacaac aattccataa
     1261 ttttgttttg gatttagtaa aataaatgat gaaaagttat ctagcgttcc tgaatgaaac
     1321 actaaatgtt catcattatt ggtaaaccag ccggaagcat atgcattggc attaggttca
     1381 ccaattgttg aagataaatt tttatgtgat tgttgaacta atgatttgta tttatcaggt
     1441 ggattaagtt ggaattttat ccaatgttcc aaatcttcag ttgatgtcat catatatgct
     1501 gatggtgtat cccaaaggtt aaattcaggt ttagagacga caggtgtcga accttgtaat
     1561 tcatagccaa tagcatcatg ttttgatttg taattggttt gtttgaatga tgtatgtgtc
     1621 atatgcaaag gcttgagcca tgaatttgta atatattttg tataggattg cttcgtaacg
     1681 ttttggataa ttaaacctaa taaatcatag ttcatatttg agtattcaaa ttcttctccg
     1741 ggcttatgat gtaattcatc acccataatt gcacgggtta catcatttaa acgattattt
     1801 ttgcttgtca cagaatcttc gcttgtaata tcactaggta taccacttgt ttgagccaaa
     1861 agttgcttaa tcgtaatagt ttcattttga ccattatagt tcattttaaa atgaggcaca
     1921 tgtttggata cggcatcatt taagtttaat cgaccttctt gagctaattt taaaattgca
     1981 agacctgtga aagctttcgt attagaagca atttcatact ttgttgtggg agaagctttg
     2041 acttttttat caacatcttg ataaccataa cctttattta aaaaaacttt gccatttttt
     2101 actattaaaa ttgatgctcc aggaatgtgt cccttttgta aatcatgctc gataattgta
     2161 tctatttgtt gttgcgaatc attggttaac cgtgtcttcg tattgctatt taatgttata
     2221 tatatagcaa ttgaaatggc gactaaaatg ataatagaaa tggatagaaa atacagtttt
     2281 ttagtagtca taaacgttcc tttttatatg ttaaatttac ttaagtgcct aatgagattt
     2341 attttattat accagttcta ttgaataaat taattaaaga ggtatattta ccattatttt
     2401 tgacacctta tatgttaata ggtgtataat tacataaaaa atattaatta actctctata
     2461 ctttttggaa agataggcgt atagtgatat ccatcaaaaa ctataaaaag gaataggaga
     2521 ttatgttcaa tcaatttaaa agacttatta tagggcaacc taaaaaaaac agagaactaa
     2581 aagacgaaaa gatatcgaag tttaaagggt tagcgattct ttcgtcggat gcattatctt
     2641 cagtggctta cggtcccgaa caaatactga ttacactatc agtagtgggt gcagttgcaa
     2701 cttggtatac tttaccgatt gcaggtgctg ttctgatctt attagctgct ttaattatgt
     2761 cttataggca aattatttat gcatatccta aaggtggcgg cgcgtatatg gtgtccaaga
     2821 ccaatttagg agaaaaatgg ggattgcttg caggaggctc attattagtc gattacatac
     2881 tgactgtcgc tgttagtata tcatctggtg ctgatgcatt tgttgctgca ttcccaagtt
     2941 tatatgggca taaagtactg attgcatgtt tacttgtact ttttatttta atattaaatt
     3001 tacgtggttt aact
//
LOCUS       DANRDI010000230         2987 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.233, whole genome shotgun sequence.
ACCESSION   DANRDI010000230 DANRDI010000000
VERSION     DANRDI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.233"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(155..379)
                     /locus_tag="PZ377_002688"
     CDS             complement(155..379)
                     /locus_tag="PZ377_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359536.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
     gene            complement(336..482)
                     /locus_tag="PZ377_002689"
     CDS             complement(336..482)
                     /locus_tag="PZ377_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359537.1"
                     /translation="MKLFYIVFLIIIWLNIFLGNEIIHTLTVLITTLYIVNSRKGIKN
                     DRVE"
     gene            1155..2114
                     /gene="hyl"
                     /locus_tag="PZ377_002690"
     CDS             1155..2114
                     /gene="hyl"
                     /locus_tag="PZ377_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HDM6359538.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
     gene            2591..2824
                     /locus_tag="PZ377_002691"
     CDS             2591..2824
                     /locus_tag="PZ377_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359539.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT         1129 a          399 c          402 g         1057 t
ORIGIN      
        1 ataattgaaa aatgcttgtt acatgggcat tttcattcgg tcaactacta ccaatataat
       61 attgtagagc ctaagacatt gatttattat gtcttaggct ctattccttc atttaatgat
      121 taaattatta tagcaatact ttattgtccc atgattagtg ttcttttaat gagacatagt
      181 aactataaag tttaataatc gttctaaatc tagcatcttc tagtttggtt ttcccatttc
      241 ttaaatcttg tactgtttga tatggaactc ctgaattttt cgaaatttta tagcctgatt
      301 cagactcaaa caaagattga agagagttta taatattatt caactctgtc atttttaatc
      361 ccctttcttg aattaacaat atacaatgtt gttattaaaa cagtcagtgt atggatgatt
      421 tcatttccta aaaatatatt cagccatata ataataagaa atacgatata aaatagtttc
      481 attttaatcc cctatcatat ttttattaat caaatttcaa taagattcat aattaaaagc
      541 taacctatac tcaaaatttt cttataatgc ctctaactaa aaacctacat tttattttac
      601 atataatatt ttacactata ttaaaaatac atacgatact ttttcgttat ctattagata
      661 tttctatgta atggcaaaat ttattcccga cgaaattcca aacataatta ataccctttt
      721 tctctatttc tatttattaa tttacactag ttaatatata gttaattttt atttaatagt
      781 taattaattg atttaattct aagatatttg tatttttata ttgtaatcga ttacattttt
      841 aaatcaaaca aatcattagt tttaaagaaa ttaatcaata aaattagcta tgtcttttcc
      901 ttgtttcata aagaatttat tcaactttga ctaaccctcg aaattgaaat gcttcctttc
      961 aaattttaaa taaaagttaa aaacatattc ttaaaataat cactcatcat cactcagtaa
     1021 tttatcagtt gctacattaa atatactaat ataaacgatt ataaatattt gatatgtctc
     1081 aactgcaata ttctaaattg acatattgat tattgtttcc tcaaatcgtt ttaaaaaata
     1141 gaaggatgat gaaaatgaaa acacgtatag tcagctcagt aacaacaaca ctattgctag
     1201 gttccatatt aatgaatcct gtcgctaatg ccgcagattc tgatattaat attaaaaccg
     1261 gtactacaga tattggaagc aatactacag taaaaacagg tgatttagtc acttatgata
     1321 aagaaaatgg catgcacaaa aaagtatttt atagttttat cgatgataaa aatcataata
     1381 aaaaactgct agttattaga acgaaaggta ccattgctgg tcaatataga gtttatagcg
     1441 aagaaggtgc taacaaaagt ggtttagcct ggccttcagc ctttaaggta cagttgcaac
     1501 tacctgataa tgaagtagct caaatatctg attactatcc aagaaattcg attgatacaa
     1561 aagagtatat gagtacttta acttatggat tcaacggtaa tgttactggt gatgatacag
     1621 gaaaaattgg cggccttatt ggtgcaaatg tttcgattgg tcatacactg aaatatgttc
     1681 aacctgattt caaaacaatt ttagagagcc caactgataa aaaagtaggc tggaaagtga
     1741 tatttaacaa tatggtgaat caaaattggg gaccatatga tagagattct tggaacccgg
     1801 tatatggcaa tcaacttttc atgaaaacta gaaatggctc tatgaaagca gcagataact
     1861 tccttgatcc taacaaagca agttctctat tatcttcagg gttttcacca gacttcgcta
     1921 cagttattac tatggataga aaagcatcca aacaacaaac aaatatagat gtaatatacg
     1981 aacgagttcg tgatgactac caattgcact ggacttcaac aaattggaaa ggtaccaata
     2041 ctaaagataa atggatagat cgttcttcag aaagatataa aatcgattgg gaaaaagaag
     2101 aaatgacaaa ttaatgtaaa ttatttgttc atgtacaaat aaatataatt tataacttta
     2161 gccgataact tcagaaatga tgtttttcgg ctaattttta tactgaattt agtttaatta
     2221 acttatgata tttttaattt aaactatcta cttgatttgc tttcctgact taaaaattag
     2281 actaaacatg acactatatg aggtaatttt taaaataaga aatttgaatt ccatgttgtc
     2341 aaacccttta tataaaagtt ttgaaattgt tcaaaacttt ttgaacacat tatgtgttaa
     2401 tatatttgca gaattatttt aaaatcgttt taaaactata ctgaaataag aaatttaaaa
     2461 gtataattag tacatataat tgaaaaagat ttctagaaat acaattaatg ttcatctaga
     2521 tattgtacga agttgtatga ataagttaaa cttaagttga atcaatttaa atgttcaagg
     2581 aggttaacaa atggttttta atgattggga aattaacgtt gtatactcag gagagcatga
     2641 ggttgtaaca aacgaaaaga gtttatttgt cattgacgaa ttttacgatg ttgcaatcgc
     2701 aatctattat ttagatggaa aattaaaagt agctcatgtc aactatggtt ctgattttac
     2761 aattgacgta gctaataaag tttttgaatt gaatattcaa aatccaaatg ttgatgagca
     2821 ttaaatatta aaaagcattt agaatcataa agagcatact atatatagcc ttttacatgt
     2881 aaagcaaatg aaaattagat aatctaatta tttcaacatt aaacaatata tcattataaa
     2941 gaagttttta aaatagaagt ataaggtggt gaacaaaaat taacatt
//
LOCUS       DANRDI010000231         2985 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.234, whole genome shotgun sequence.
ACCESSION   DANRDI010000231 DANRDI010000000
VERSION     DANRDI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.234"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..100
                     /locus_tag="PZ377_002692"
     CDS             <1..100
                     /locus_tag="PZ377_002692"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDM6359540.1"
                     /translation="ENSGSNNATLFGGLFAALGSLLLFGRRKKQNK"
     gene            494..>2985
                     /locus_tag="PZ377_002693"
     CDS             494..>2985
                     /locus_tag="PZ377_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDM6359541.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPK"
BASE COUNT         1259 a          437 c          487 g          802 t
ORIGIN      
        1 tgaaaatagc ggctcaaata acgcaacgtt atttggcgga ttattcgcag cattaggatc
       61 attattgtta ttcggtcgtc gtaaaaaaca aaataaataa tacagcttat accaggtccg
      121 tgaggcctgg ttttttattt caaaatttat gaaatgtatc ttatgtttga gattaaatag
      181 tgcaattttt taaaaaatat aacataaatc aacataaatt taattcatct ttacattaaa
      241 agtacaaacg aataagaaaa gcaattaaac acagtatcaa accatttaaa acaattgata
      301 taaaaaacaa acgaataatt aaaaagtact agaattaatt tattcaaacc aaagtgtaaa
      361 ataacagtct tagataaata aatttattta aagtattgtg ctttatctaa aaatgtatta
      421 cgatgggaat acaaattttt atatgtaaaa atattaattt tttgcaaata ttgatatttt
      481 aaaggagatt tatatgatta acagggataa taaaaaggca ataacaaaaa agggtatgat
      541 ttcaaatcgc ttaaacaaat tttcgattag aaagtatact gtaggaactg catcgatttt
      601 agtaggtacg acattgattt ttggtctagg gaaccaagaa gctaaagctg ctgaaaacac
      661 tagtacagaa aatgcaaaac aagatgatgc aacgactagt gataataaag aagtagtgtc
      721 ggaaactgaa aataattcga caacagaaaa taattcaaca aatccaatta agaaagaaac
      781 aaatactgat tcacaaccag aagctaaaaa agaatcaact tcatcaagta ctcaaaaaca
      841 gcaaaataac gttacagcta caactgaaac taagcctcaa aacattgaaa aagaaaatgt
      901 taaaccttca actgataaaa ctgcgacaga agatacatct gttattttag aagagaagaa
      961 agcaccaaat aatacaaata acgatgtaac tacaaaacca tctacaagtg aaccatctac
     1021 aagtgaaatt caaacaaaac caactacacc tcaagaatct acaaatattg aaaattcaca
     1081 accgcaacca acgccttcaa aagtagacaa tcaagttaca gatgcaacta atccaaaaga
     1141 accagtaaat gtgtcaaaag aagaacttaa aaataatcct gagaaattaa aagaattggt
     1201 tagaaatgat agcaatacag atcattcaac taaaccagtt gctacagctc caacaagtgt
     1261 tgcaccaaaa cgtgtaaacg caaaaatgcg ctttgcagtt gcacaaccag cagcagttgc
     1321 ttcaaacaat gtaaatgatt taattaaagt gacgaagcaa acaatcaaag ttggcgatgg
     1381 taaagataat gtggcagcag cgcatgacgg taaagatatt gaatatgata cagagtttac
     1441 aattgacaat aaagtcaaaa aaggcgatac aatgacgatt aattatgata agaatgtaat
     1501 tccttcggat ttaacagata aaaatgatcc tatcgatatt actgatccat caggagaggt
     1561 cattgctaaa ggaacatttg ataaagcaac taagcaaatc acatatacat ttacagacta
     1621 tgtagataaa tatgaagata taaaatcacg cttaactcta tattcgtata ttgataaaaa
     1681 aacagttcca aatgagacaa gtttgaattt aacatttgct acagcaggta aagaaacaag
     1741 ccaaaatgtc actgttgatt atcaagatcc aatggtccat ggtgattcaa acattcaatc
     1801 tatctttaca aaattagatg aagataagca aactattgaa caacaaattt atgttaaccc
     1861 attgaaaaaa tcagcaacca acactaaagt tgatatagct ggtagtcaag tagatgatta
     1921 tggaaatatt aaactaggaa atggtagcac cattattgac caaaatacag aaataaaggt
     1981 ttataaagtt aactctgatc aacaattgcc tcaaagtaat agaatctatg attttagtca
     2041 atacgaagat gtaacaagtc aatttgataa taaaaaatca tttagtaata atgtagcaac
     2101 attggatttt ggtgatatta attcagccta tattatcaaa gttgttagta aatatacacc
     2161 tacatcagat ggcgaactag atattgccca aggtactagt atgagaacaa ctgataaata
     2221 tggttattat aattatgcag gatattcaaa cttcatcgta acttctaatg acactggcgg
     2281 tggcgacggt actgttaaac ctgaagaaaa gttatacaaa attggtgact atgtatggga
     2341 agacgttgat aaagacggtg ttcaaggtac agattcaaaa gaaaaaccaa tggcaaacgt
     2401 tttagttaca ttaacttacc cggacggtac tacaaaatca gtaagaacag atgctaatgg
     2461 tcattatgaa ttcggtggtt tgaaagacgg agaaacttat acagttaaat tcgaaacgcc
     2521 aactggatat cttccaacaa aagtaaatgg aacaactgat ggtgaaaaag actcaaatgg
     2581 tagttcggtt actgttaaaa ttaatggtaa agatgatatg tctttagata ctggttttta
     2641 caaagaacct aaatacaact taggtgacta tgtatgggaa gatactaata aagatggtat
     2701 ccaagatgca aatgagccag gaatcaaaga tgttaaggtt acattaaaag atagtactgg
     2761 aaaagttatt ggtacaacta ctactgatgc ctcgggtaaa tataaattta cagatttaga
     2821 taatggtaac tatacagtag aatttgaaac accagcaggt tacacgccaa cggttaaaaa
     2881 tactacagct gatgataaag attctaatgg tttaacaaca acaggtgtca ttaaagatgc
     2941 agataatatg acattagaca gtggtttcta taaaacacca aaata
//
LOCUS       DANRDI010000232         2963 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.235, whole genome shotgun sequence.
ACCESSION   DANRDI010000232 DANRDI010000000
VERSION     DANRDI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.235"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(194..1129)
                     /gene="trxB"
                     /locus_tag="PZ377_002694"
     CDS             complement(194..1129)
                     /gene="trxB"
                     /locus_tag="PZ377_002694"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDM6359542.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1196..2635)
                     /locus_tag="PZ377_002695"
     CDS             complement(1196..2635)
                     /locus_tag="PZ377_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDM6359543.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            complement(2643..>2963)
                     /locus_tag="PZ377_002696"
     CDS             complement(2643..>2963)
                     /locus_tag="PZ377_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDM6359544.1"
                     /translation="INIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALV
                     DEFRYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIK
                     MIRR"
BASE COUNT          958 a          568 c          377 g         1060 t
ORIGIN      
        1 taaagttata agtttaatat aacatataaa aaaactagaa ctcattgaaa cgcacaaatg
       61 atacgtacta agttctagtt ttctatataa tttgctttaa tttaaaagta agacattatt
      121 ttaacgctgt gatgacacta ttaaaataaa tatccaaata atttacagct caacatctta
      181 attcgacttc gaattaagct tgatcgttta aatgttcaat atattccgct gcactttgcg
      241 ctgcaatact accatcgcca gtagcagtga caatttggcg taaacctttg tcgcgaacat
      301 ctcctgctgc aaaaatacct ggtactgatg ttgtcatatc atcttttgtt acaatataac
      361 caacatcatt tgtaatacct aagtctttaa atggcgctgt taatggtttc ataccaatat
      421 agatgaatac accatcagcc tcgtgtgttt cttctgaacc atcttttgta gacgttaatg
      481 tcacagaacc cactttgccg tctttttcat taattgattt caaagtatga ctccaaataa
      541 agtcgatttt atcatttttg aatgctctat cttgtaaaat acgctgtgca cgtaactcat
      601 cacgacggtg aacgattgtt actttgtcag caaatttagt taagaatgtt ccctcttcta
      661 ctgctgaatc accaccaccg ataacgaata ggcgtttatt tttaaagaat gcaccatcac
      721 atactgcaca ataacttaca ccgcgtccac caagttcttg ttcacccgga acaccaattt
      781 tcttgtattc tgcacctgta gcaataataa ccgctttcgc tgttaattct ttattaccaa
      841 agttaatcac tttatattcg cctttatctt ctacagattt aatatctcca tattgataaa
      901 ctgcaccaaa ctttttagcg tgttcaaaca tttttgtaga taaatctgga cctgtaatca
      961 tttcgaaacc agggaagttc tctacttctt ctgtattagc catttgaccg cctggaatac
     1021 ctctttcaat cataactgtt tttaaattag cacgtgatgc gtatactgca gcagtcatac
     1081 cagctggacc tgcaccgata attgctatat caaaatctat ttcagtcatt ttattaacgc
     1141 ctcctcatta ttaatcatta tgcgcattat ataataaatc taacttttca taaatctata
     1201 tgctcaagag aaattcaatc attttgttca gtttatattg tgttatgcct aaccatgttg
     1261 taatttgctt ctttgtaacg tttcgaggtt gatttttaaa atacaaataa ataaacgcac
     1321 cgatatatgg ctcaacatca gttaaatcta ctttttcagc aattatgagt tcaccttgat
     1381 taatccatgc aaccattaca tcattttcac ttacaaataa ttcattatgg taaagcgtta
     1441 ataagccgcg atgaatgaag tctaatttat tgagcgttaa accttgaact aaatacgtta
     1501 aatacaattt ctcataatta tttagatttt ccaaaacctg ccaaatactt tccgtcatca
     1561 caatttcttt accatttaat tgatccaata aaaaaatacc atataaacga taatgactgt
     1621 catcactttg aagtaatggc aaaatatgtt ggtcaaaaac ttctttgcta ttttcaatta
     1681 cccaaggcgc atgtccaatt tccactttag aaatttgctt caatttatcc cagtagtaat
     1741 gactttcgtc ttcttcacct aaataataat aattatatgc taaagcattg tacatttgaa
     1801 ttgataaaaa tttccctttt ttatataaag ggtacaacaa ttgttgtgat gcacgatact
     1861 gctttaaata acttaataca atacctaatt taaaactttc atcgtcattc ataggtacaa
     1921 ctttgtttaa tatttttaaa tatttttgat attgttcatt ttccttagtg ttataaagta
     1981 gcaaagtata atggcataat gcatatatat ctgtactatc ctcttgtaat agtgcttcgt
     2041 acaatgcttt agccgtatca tattcaccga gatataaata acacattgcc aataaattac
     2101 gtactacacg atgatcttga acttcttgtg gttgatgtaa gacataattc tttgcatctt
     2161 ttaatcgacc ttgagaaaaa agatattgga atagtatttg aactacaaac aattgagctt
     2221 caagttcaat ttgttcttca ctttgatatt tcacatcaaa catttgatct aattcatctc
     2281 tataatcttg gtcgtcgcta acaataacat aattaatacc aaacaagaat gccttgtttg
     2341 gttcgttaac ttcaatgttg agctgactca attcataaaa gctatcttct agatgcctat
     2401 tataaataat attgtcataa aataaatgtt ctgctttttt agcaatacca agttgcacta
     2461 gacattgtgc ataatcaatt tgaatttcca tatcatcagg tgacaattct aacacttttt
     2521 caaagtattc agcagctcgt ttatattctc tttgtttaaa cttctgttta gccattttac
     2581 gataaaatgc atcatcaaaa gtcattggaa ttacattatt attcttttgc gccataaaat
     2641 tgtcacctcc taatcatttt tatatacata gggttgccat atgcaaatcc attatccggt
     2701 atatcttttg aaacaaccgt accagctgca acttttacat tgtcaccaat cgttataccg
     2761 ggtaaaatgg tagcatttgc accaatcaaa gtgttagatc ctatcgtcac tggtccataa
     2821 cgaaattcat caactaatgc ttcatgcgtc aaaattgtta cattgtaacc aataacactg
     2881 ttactaccaa tcgtaatcag ttctgggtaa aaaatatcta acattacttt ataagctatc
     2941 gacgattgat taccgatatt aat
//
LOCUS       DANRDI010000233         2920 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.236, whole genome shotgun sequence.
ACCESSION   DANRDI010000233 DANRDI010000000
VERSION     DANRDI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.236"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(274..414)
                     /locus_tag="PZ377_002697"
     CDS             complement(274..414)
                     /locus_tag="PZ377_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDM6359545.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
     gene            complement(411..980)
                     /gene="agrB"
                     /locus_tag="PZ377_002698"
     CDS             complement(411..980)
                     /gene="agrB"
                     /locus_tag="PZ377_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HDM6359546.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            1216..1350
                     /locus_tag="PZ377_002699"
     CDS             1216..1350
                     /locus_tag="PZ377_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDM6359547.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(1947..2732)
                     /locus_tag="PZ377_002700"
     CDS             complement(1947..2732)
                     /locus_tag="PZ377_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDM6359548.1"
                     /translation="MKVQIYQLPIFFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT         1089 a          435 c          404 g          992 t
ORIGIN      
        1 cataatcaac aaaatagaat accatttgat tttgacaaaa tacattataa taataaatga
       61 agttaatatg attaaggacg cgctatcaaa cattttaaat agaaataacg ataatgtcga
      121 aattactatg atgaaaaaat aatcaagttt actgtactta ataccactaa ttatagctgg
      181 tattgtaaac attaatatca tttgagttaa tacgaataaa acaaaattat aactatttaa
      241 taattccacc tactatcaca ctctctattt aaattattcg tgtaattgtg ttaattcttt
      301 tggtacttca acttcatcca ttatgaagtc acaagtacta taagctgcga tgttaccaat
      361 gttttttaaa atcccagtaa taaaatcaaa aaataagtta aataatgtat tcattttaag
      421 tcctccttaa taaagaaaat aggtaataat gtaatagctt ctattatgat gcctaattga
      481 atgaattggg caaatggctc tttgatgata agtgtgataa tgaaaagggt taaactaaca
      541 ataatcgcat aatatttttt tcgtttaata agtcgcacag gaatgggctt ctttttagtt
      601 gctgcaggag catatactga gattacacct aaagaaataa ctgttaaaat aatcataatt
      661 aaaaagttaa tatgaaaatt tactattact aaaggtaaaa gtataaatag tataatactt
      721 tctacataac accaaaaaga agaaggtgca tgtgcaccat gtgcatgtct tcttattaaa
      781 taaaatgtta aattcgtaat taacgtaaac agaaaaatgt ttaaaatata ggcaatagta
      841 tacataacaa ttaatttacc tatattttta gctaagacct gcatccctaa tcgtacttgc
      901 aaaaattgaa tatgatctaa gttatttctc ttttgaagat acgtggcaaa ctggtcaatt
      961 ttattatcaa aataattcaa ttttacacca ctctcctcac tgtcattata cgatttagta
     1021 caatctttta tcattatatt gcctaactgt aggaaataaa tacttaactg ttaaatgtaa
     1081 tttgtattta atattttaac ataaaaaaaa ttacagttaa gaataaaaaa cgactagtta
     1141 agaaaaattg gaaaataaat gcttttagca tgttttaata taactagatc acagagatgt
     1201 gatggaaaat agttgatgag ttgtttaatt ttaagaattt ttatcttaat taaggaagga
     1261 gtgatttcaa tggcacaaga tatcatttca acaatcggtg acttagtaaa atggattatc
     1321 gacacagtga acaaattcac taaaaaataa gatgaataat taattacttt cattgtaaat
     1381 ttgttatctt cgtatagtac taaaagtatg agttattaag ccatcccaac ttaataacca
     1441 tgtaaaatta gcaagtgagt aacatttgct agtagagtta gtttccttgg actcagtgct
     1501 atgtattttt cttaattatc attacagata attatttcta gcatgtaagc tatcgtaaac
     1561 aacatcgatt tatcattatt tgataaataa aatttttttc ataattaata acatccccaa
     1621 aaatagattg aaaaaataac tgtaaaacat tcccttaata ataagtatgg tcgtgagccc
     1681 ctcccaagct cgcggccttt tttgtaatga agaagggatg agttaatcat cattatgaga
     1741 cccgccgtta aaatatatga ataagtctaa tgttggaaaa ggtcaaaaaa ttaatcaatt
     1801 taattaagaa aatcattcat ttgcaaaggg cgaaatgggt tcttactgag ttatctatta
     1861 taaaaaaata aacatagact tatgaaaaat ctctcataaa tctatgttta gtcatgacat
     1921 gtgttaaata ttatttcggg cgcttcttat ttatacaaat ctaatttaat acttttaaat
     1981 acaggtatat tttcgcgttg ctgttctact tcatttaagt ttaaatctac agtcaaaata
     2041 tctgcggatt catttaattc tccaactaaa tctccatttg ggtttataac tatcgaatga
     2101 ccagcatatt ctgtgttacc atcgaatcca gtgctattag ttccaatgac aaacatatta
     2161 ttttcaattg cacgtgcctt tagtaatgaa tgccaatgtt gaagacgtga cataggccat
     2221 tgcgccacat aaaatgcaat tttagcacca ctacgagcag gatatcttaa taattctgga
     2281 aaacgtaaat cataacagat aagttgggtc acataagtac cgtcagacaa ttgaaagggt
     2341 tcagctacgt attcgccagc ggttaaaaat tcatgctctc ttaacatagg aactaaatga
     2401 actttgtcgt attcattaat cagctggcca cttttattca cactaaaagc tgtattaaat
     2461 atttgattgt ttctaatgtt agaaactgac ccagctacga tatcgacttt atatttttca
     2521 gctaaatgtt taataaatga aaaactttgt cctagattat tatctgcttt ttcatttaaa
     2581 tgctctaaat catagccatt attccacatt tcaggtaaaa cgactacatc tacttcagca
     2641 ttcatatttt tttcgaacca ttgcgttatt tgagtttcat ttttagaact atctccaaaa
     2701 aaaatcggta attgataaat ttggactttc ataacatcac atccttgata gatcttatat
     2761 ataacttact aaaagttatg ttgaaacgca aaaaacgagc acaagacata aaatcaaagt
     2821 cctaggctct acaaagttat attgacagta gttgatgggg ccccaacata gagaaattgg
     2881 aacaccaatt tctacagaca atgcaagttg gggtgggctc
//
LOCUS       DANRDI010000234         2918 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.237, whole genome shotgun sequence.
ACCESSION   DANRDI010000234 DANRDI010000000
VERSION     DANRDI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.237"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            171..1100
                     /gene="hlgA"
                     /locus_tag="PZ377_002701"
     CDS             171..1100
                     /gene="hlgA"
                     /locus_tag="PZ377_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDM6359549.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            1667..2614
                     /gene="hlgC"
                     /locus_tag="PZ377_002702"
     CDS             1667..2614
                     /gene="hlgC"
                     /locus_tag="PZ377_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDM6359550.1"
                     /translation="MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            2616..>2918
                     /locus_tag="PZ377_002703"
     CDS             2616..>2918
                     /locus_tag="PZ377_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin family pore-forming toxin"
                     /protein_id="HDM6359551.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSK"
BASE COUNT         1186 a          451 c          424 g          857 t
ORIGIN      
        1 aaatctttta aacataacat aatgcaaaaa catcatttaa caatgctaaa aatgtctctt
       61 caatacatgt tgatagtaat taacttttaa cgaacagtta attcgaaaac gcttacaaat
      121 ggattattat atatatgaac ttaaaattaa atagaaagaa agtgatttct atgattaaaa
      181 ataaaatatt aacagcaact ttagcagttg gtttaatagc ccctttagcc aatccattta
      241 tagaaatttc taaagcagaa aataagatag aagatatcgg ccaaggtgca gaaatcatca
      301 aaagaacaca agacattact agcaaacgat tagctataac tcaaaacatt caatttgatt
      361 ttgtaaaaga taaaaaatat aacaaagatg ccctagttgt taagatgcaa ggcttcatta
      421 gctctagaac aacatattca gacttaaaaa aatatccata tattaaaaga atgatatggc
      481 catttcaata taatatcagt ttgaaaacga aagactctaa tgttgattta attaattatc
      541 ttcctaaaaa taaaattgat tcagcagatg ttagtcagaa attaggctat aatatcggcg
      601 gaaacttcca atcagcgcca tcaatcggag gcagtggctc attcaactac tctaaaacaa
      661 ttagttataa tcaaaaaaac tatgttactg aagtagaaag tcagaactct aaaggtgtta
      721 aatggggagt gaaagcaaat tcattcgtta caccgaatgg tcaagtatct gcatatgatc
      781 aatacttatt tgcacaagac ccaactggtc cagcagcacg agactatttc gtcccagata
      841 atcaactacc tcctttaatt caaagtggct ttaatccatc atttattaca acattgtcac
      901 acgaaagagg taaaggtgat aaaagcgagt ttgaaatcac ttacggcaga aacatggatg
      961 ctacatatgc ttacgtgaca agacatcgtt tagccgttga tagaaaacat gatgctttta
     1021 aaaaccgaaa cgttacagtt aaatatgaag tgaactggaa aacacatgaa gtaaaaatta
     1081 aaagcatcac acctaagtaa acagttcaat catcttaaaa aatcctggga cacttcatac
     1141 ttgtctcagg attttttaac aaattgaatc agccttataa cattaaatta ttttatcgta
     1201 cattaaattt aataataaca actgattttt ataagaataa agtatcgaac catagtagat
     1261 acacaaataa tacaaatgaa acaatttaac ttgaaagctt aaataaatat tatcaagtta
     1321 ataaacaatt aatttttaga tggattcatc aaaaatcgta aaaagcacaa tttgtatttt
     1381 acaaacatta attaaaaaag aaagcaagac attcgtgcaa tcggttacct taaattgttt
     1441 acaactgtca acaataccaa ggttttatta actatatttc tcacaaaatt agcttttagc
     1501 attccaaaca aaaaaggtta aatcgaacgg aattatggca tttttaactt aattgtaaaa
     1561 aaagttgata atggtcaatt gttaatgaac agttaattat aataacgccc aaaatatatt
     1621 attatttaat taagttaaat aaaattatag aaataaagtg aaacttatgc ttaaaaataa
     1681 aatattaact acaactttat ctgtgagctt acttgcccct cttgccaatc cgttattaga
     1741 aaatgctaaa gctgctaacg atactgaaga catcggtaaa ggaagcgata tagaaattat
     1801 caaaaggaca gaagataaaa caagtaataa atggggcgtg actcaaaata ttcaatttga
     1861 ttttgtaaag gataaaaaat ataacaaaga tgctttgata ttaaagatgc aaggattcat
     1921 tagctctaga acaacatatt acaactataa aaaaactaat catgttaaag ctatgcgatg
     1981 gccattccaa tataatattg gtttaaaaac aaatgataaa tatgtttctt taattaatta
     2041 tttacctaaa aataaaattg aatctacaaa cgtgagtcag acattaggat acaatatcgg
     2101 tggtaatttc caatcagccc catcactcgg tggtaatgga tcatttaact attctaaatc
     2161 gattagctat acacaacaaa attatgtaag tgaagtagaa caacaaaact caaaaagtgt
     2221 tttatggggc gtcaaagcga attcattcgc cactgaatca ggtcaaaaat cagcctttga
     2281 tagcgattta tttgtaggct acaaacctca tagtaaagat cctagagatt atttcgttcc
     2341 agacagtgag ttaccacctc ttgtacaaag tggatttaac ccttcattta tcgccacagt
     2401 atctcatgaa aaaggttcaa gcgatacaag cgaatttgaa attacttacg gaagaaacat
     2461 ggatgtcact catgccatta aaagatcaac gcattatggc aacagttatt tagacggaca
     2521 tagagtccat aatgcatttg taaatagaaa ctatactgtg aaatacgagg tcaattggaa
     2581 gactcatgaa atcaaggtga aaggacagaa ttgatatgaa aatgaataaa ttagtcaaat
     2641 catccgttgc tacatctatg gcattattat tactttctgg tactgctaat gctgaaggta
     2701 aaataacacc agtcagcgta aaaaaagtcg atgacaaagt tactttatac aaaacaacag
     2761 ccacagcaga ttctgataaa tttaaaattt cacagatttt aacatttaat ttcatcaaag
     2821 ataaaagtta tgataaagat actttagtac ttaaagctac tgggaatatt aactcaggct
     2881 ttgtgaaacc taatcctaat gactatgact tttcaaaa
//
LOCUS       DANRDI010000235         2909 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.238, whole genome shotgun sequence.
ACCESSION   DANRDI010000235 DANRDI010000000
VERSION     DANRDI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.238"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(149..1732)
                     /locus_tag="PZ377_002704"
     CDS             complement(149..1732)
                     /locus_tag="PZ377_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDM6359552.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(1725..2204)
                     /locus_tag="PZ377_002705"
     CDS             complement(1725..2204)
                     /locus_tag="PZ377_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359553.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            complement(2412..>2909)
                     /locus_tag="PZ377_002706"
     CDS             complement(2412..>2909)
                     /locus_tag="PZ377_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDM6359554.1"
                     /translation="FPLAITLIGQIFFYQQANGSLITYDNRIVGSKLIGQHWTETRYF
                     HGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIARMKHHVKFGNSNVTIDAATSS
                     GSGLDPHITVENALKQAPRIADARHVSTSRVADLIQHRKQRGVLTNDYVNVLELNIAL
                     DKMKD"
BASE COUNT          979 a          541 c          391 g          998 t
ORIGIN      
        1 actactaaca atggataaat ggttatctaa aatctcccaa aatagccagt tcaaaatatt
       61 aaatgcgatt aaaagtacaa gcgctatggg tatccaaaac agcacagcac ttaacctcat
      121 cactttttta gcgtgtggtg acataaaatt ataatccctc atcattttca cctcttaaat
      181 accatgattt caatttattt gcatcttcac ttcttgcgta ttttaagtta atcgattggg
      241 ctcccacacc tttagcaaca ataaagctaa aattatttaa attgttgcgt ttaagtaatg
      301 tatgttgcca agtgtcaaat cctataatgt gatgcgcttt aaaataataa atatttcgtt
      361 tcaatagctc gaaattctgg atagtaattt gttcttctgt cattttaaaa cccgcatgtt
      421 tgacataaag atatcctttg atcacaaata aaccaataat gactattgtt ataatcgtaa
      481 ataacaataa taattgattc caaaaataac agcctatacc tgccatagct atgacaataa
      541 tactaggtat taaaaagtgt ctgtggaaac ctgacaaagg cataccttca ttaacttgtt
      601 gataagataa atctggtact aaattctgga taatttgata tgctttgtct cgtttaataa
      661 acggcaatat cggcacacta cctgaatcat tgtcatcatc ttcgattttt ttattttcaa
      721 aatcacttgt gatgataaaa tgtattgctg tgtagccaaa taatcttcgt atatatgact
      781 gacgttcaac aacagcttgc aatcgtttcg ttggtacagt tactttcttc ttgttaaaga
      841 gaccatattc aattttaagt tgctcatttt caagcatcac cgaaaagcca taaaatctta
      901 tcattgttat aatcgttcca ataatatatg ccactattaa tactagtaaa atgatgatta
      961 atactgaaat acttacaatt tgaacccatt gactaatttc atgatttagc ttcgaccatg
     1021 ggatcaactc tcttacagcc ccgtaaatcg gtactaaagc tgctaacgtt acaccaatgg
     1081 cgccactggt cattgccata aatagtgatt ctttaaaatt catctgatat ataggaatgc
     1141 gtttattttt ctgattaagc atactatcag tgttctgcac ttcatctaag cgaccttctg
     1201 cgatgtcttc cacattacct tcaatgtcat gattacagtt gtcattcttc tcagcactag
     1261 acttttgcgc cacttctgtc ttcaactctg tttgcaattg atcaatatat cgttcaagat
     1321 attcaccttg ttttttcgaa ataacactta agacaatacc atcacttggt gttttgattt
     1381 gtaaatccac accaccaata atttgattga cgaccccttg tgtcatatca acggactgaa
     1441 tacgttttat atttaattct ttacgctttt tattaaaaat acctgttgtt aaaatgaaat
     1501 aattatcctc aatccaatat cgcgtgttca taattccgac aatttgagaa atgtatgata
     1561 ttaaaaagaa tacaaataca atacctatcc ataaatatga ttcgggattc gtataatcaa
     1621 aatctttcaa ttgaaagata atgaaaataa aaaagacgac tatgttttgt ttgatagcat
     1681 tgattatgcc attaaaatat gaaatcggat gtaatttttg aggttcagac atcactttca
     1741 acccctctca aattcgacat agttctctct tcgattattt taacatcgtc atgagacatc
     1801 atcggtaaat aaatagtatg acctgcagtc ataaatccaa ctttatacaa attaagcact
     1861 ttactaattg gattagattt aatcgacaag tattgtaaac gttcaattcg actcgtttct
     1921 tctttatata taaaaaatga tgtacgatat tgtacactta gttgatcaac tttataaaag
     1981 cgacaatgat attgccataa aggcttaata aataatttta atgtactcag agcacctaaa
     2041 accaacaaaa tataaagtaa gtaatgtggc cattcaaatc ttaaccatat aaaataaaaa
     2101 atgacataca cagctacact caatataaat tctaagccat tcgtaatgta gtaatacaac
     2161 aatgctgact taggactctt agtcaactta gtataatctg acatataccc ctctccccaa
     2221 ataaaaaatt atacggattt ataatctatt tcattttatt tttatatgat gataattata
     2281 gcatatggaa tatttcatgc taatttattc ttcctaaagg tacatctaaa aatttaatta
     2341 agcagaaagt gcttgaattg ctaaaaagac accatgtata attttatcaa catgatgcct
     2401 ttcatctata atcaatcttt catcttatca agagcgatat ttagttcaag cacattcaca
     2461 taatcatttg ttaacacacc acgctgctta cgatgttgaa tcaagtcggc cactcttgaa
     2521 gtagatacat gacgagcatc agcaatacga ggtgcttgct tcaatgcatt ttcgaccgta
     2581 atatgcggat ctaagcccga cccagaactt gttgcagcat ctattgttac atttgaattc
     2641 ccaaatttaa catgatgttt catgcgtgct attaattcgg tgtttccatt cgattcatta
     2701 cttccacctg aagatacgcc gtttttatat aatttttcag gattcatatt ataatcaact
     2761 gcactcggtc tcccgtgaaa atatcgtgtc tctgtccagt gctgtccaat caattttgat
     2821 ccaactatac gattgtcata cgtaattaaa ctgccatttg cttgttgata aaaaaatatt
     2881 tgaccaatta acgtgatagc taacgggaa
//
LOCUS       DANRDI010000236         2844 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.239, whole genome shotgun sequence.
ACCESSION   DANRDI010000236 DANRDI010000000
VERSION     DANRDI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.239"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..849
                     /locus_tag="PZ377_002707"
     CDS             <1..849
                     /locus_tag="PZ377_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HDM6359555.1"
                     /translation="TLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVT
                     KDLAINVNQFETSNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERL
                     KVCQSKMQFYKKHITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHEL
                     SHVKSHDLIFNQLYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRY
                     GESILKCSILKSQHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVC
                     SISSFT"
     gene            821..1081
                     /locus_tag="PZ377_002708"
     CDS             821..1081
                     /locus_tag="PZ377_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDM6359556.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPYLQKMAVEQVVEQAELMASQQ"
     gene            complement(1072..2679)
                     /locus_tag="PZ377_002709"
                     /pseudo
     CDS             complement(1072..2679)
                     /locus_tag="PZ377_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          906 a          412 c          437 g         1089 t
ORIGIN      
        1 acgttaattc cattaatacc attttacaaa atatcgaatt ttacattttc aaaagatatg
       61 atgaatcgaa atgtatctga cacgacttct tcggttagtc atatgttaga tggtcaacaa
      121 tcatctgtta cgaaagactt agcaattaat gttaatcagt ttgagacctc aaatataacg
      181 tatatgattc ttttgatatg ggtatttggt agtttgttgt gcttatttta tatgattaag
      241 gcattccgac aaattgatgt tattaaaagt tcgtcattgg aatcgtcata tcttaatgaa
      301 cgacttaaag tatgtcaaag taagatgcag ttctacaaaa agcatataac aattagttat
      361 agttcaaaca ttgataatcc gatggtattt ggtttagtga aatcccaaat tgtactacca
      421 actgtcgtag tcgaaaccat gaatgacaaa gaaattgaat atattattct acatgaacta
      481 tcacatgtga aaagtcatga cttaatattc aaccagcttt atgttgtttt taaaatgata
      541 ttctggttta atcctgcact atatataagt aaaacaatga tggacaatga ctgtgaaaaa
      601 gtatgtgata gaaacgtttt aaaaattttg aatcgccatg aacatatacg ttatggtgaa
      661 tcgatattaa aatgctctat tttaaaatct cagcacataa ataatgtggc agcacaatat
      721 ttactaggtt ttaattcaaa tattaaagaa cgtgttaagt atattgcact ttatgattca
      781 atgcctaaac ctaatcgaaa caagcgtatt gttgcgtata ttgtatgtag tatatcgagc
      841 ttcacatgaa acagctaaag aagctttggg cgataaagag ttaagagcca ttgcacatga
      901 gttaactaaa acagttaagg ataacatgag tgttgattgg tctaaacgag acagtgctaa
      961 agctaaaatg agagttcaag ttagacgcct attaaagaaa tatggctatc caccatatct
     1021 tcaaaaaatg gctgtggaac aagttgtaga gcaagcagaa ttaatggcaa gtcagcaata
     1081 aaaaaataaa tcataatgag tccgggacat aaagttcttg gataagtgaa aaaagacaat
     1141 ttctattgaa ataatataga aattgtcttt tttataaatt ttttgattat tttcagctcg
     1201 ttgagctact acttttctta tattaagtgc cattaataca aaaccaagtt ctcttttgac
     1261 tttattgagt cctcggacag acatccgagt gaaacccaaa atagccttca taaatccaaa
     1321 aacaggttcc acatcaattt ttctttgact gtagatattt tttgtttctg gttctgaaag
     1381 ctttttgtta atttgggatt taaaatattc ccagttataa ttcttcatta tttttttgtt
     1441 tgtttttgaa ttgaagttca tacattgatt tttcagagga cattctgaac aatcatcaca
     1501 ttcatataat ttgaagtctc gcttataacc atacttatca tgacgatagg catatctttt
     1561 aaaacctagc cgtttattat tcggacaaat gaattcgtca ttaatttcgt catagttcca
     1621 attttgagta ttaaagatgt cacttttata ttttttagtt ttatctttta taaacattcc
     1681 atatgttatg agtggcgttc gattaaagtc atctataatt gccttataat ttgattcact
     1741 accataacct gcatcagcta caatatattc aggtaaatga ccgtaggtct cttgaattga
     1801 atttaaaaat ggaatcatcg ttctagtatc cgttggattt tgatacacat tataagataa
     1861 aacaaattgg gaatttgttg ctatttgtaa attataccct ggcttaagtt gtccattttt
     1921 catgtgatct tctttcattc tcataaatgt cgcatcataa tctgtcttag aataactatt
     1981 tctatccttt aaaatagatt tttgaaattc gtatcgatac tttcgctcaa aataatcatt
     2041 gatttgcttt ttgtattttt tgattttagt tcttttgaga cgtatttgtt ttcttgtttt
     2101 agtacatttt tcattgttga tatgttggtt taaatcttcg atttctttat ctaagtgact
     2161 accaatcaaa tctatttctt cttttgttaa ttcattatca tgatcttctt taatttccgg
     2221 tatgatttta ttggttacca attcatggta gagggcttta gaatcctcat tcatctttga
     2281 ttcatggttt tgaatactct ttttccatac aaatgtatat cgattggcat ttgcttcaat
     2341 ttttgtacca tcaataaaaa tagctttatc atctataaga ttttgtttta cacactgact
     2401 gtaaaattga ataaataaag attctaataa agcatctact tttggattta ctctaaattg
     2461 attaattgtt ttataagaag gtttttgatt ttgtgatagc cacatcattc ggatgctatc
     2521 attaagcatt ttttctattt tacgacctga gaatacagat tgtgtgtagg catatagaat
     2581 cacttttaac atcattttag gatggtacga agttgcacca cggtgatgtc tgaattcgtc
     2641 gaattcattg tcaggaattg tttcaacaat atcatttaca gtaaaacgat gttgatttgt
     2701 tttgtttcca tattgacctc catgtatttg ctatgatttc aaaatccatt tttgacgtgc
     2761 cttagggttg agtggatgca taatttcatt tgttactgga ttgatgagct tttttacttt
     2821 ctttttatga ggttttaaca tttc
//
LOCUS       DANRDI010000237         2834 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.240, whole genome shotgun sequence.
ACCESSION   DANRDI010000237 DANRDI010000000
VERSION     DANRDI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.240"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..702
                     /gene="dat"
                     /locus_tag="PZ377_002710"
     CDS             <1..702
                     /gene="dat"
                     /locus_tag="PZ377_002710"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDM6359557.1"
                     /translation="ERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQAT
                     RGVAERNHSFPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLL
                     GNVLAKEYAVKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIV
                     IKKIAEDYNIPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITR
                     QLQEGFEKYIESHSI"
     gene            1252..2043
                     /locus_tag="PZ377_002711"
     CDS             1252..2043
                     /locus_tag="PZ377_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDM6359558.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            2058..2702
                     /gene="trmB"
                     /locus_tag="PZ377_002712"
     CDS             2058..2702
                     /gene="trmB"
                     /locus_tag="PZ377_002712"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDM6359559.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
BASE COUNT         1019 a          368 c          539 g          908 t
ORIGIN      
        1 gaaagatttt tacgtagtgc caatgagatt ggtttagatt taaattattc tgtagaagaa
       61 ttaattgaac tatctcgtaa attagttgat atgaatcaaa ttgaaactgg ggcaatttat
      121 attcaagcaa cgcgtggtgt agctgaaagg aatcatagct tcccgacacc tgaagtagaa
      181 ccagcaattg ttgcttatac aaagagttat gatcgtcctt atgatcattt agaaaatggt
      241 gtgaatggtg ttaccgttga agatatccga tggttacgtt gcgacattaa aagcttgaac
      301 ttattaggaa atgtattagc aaaagaatat gctgtgaaat ataatgcagt tgaagcaatt
      361 caacatcgag gtgaaactgt aactgaagga tcttcaagta atgcttatgc aattaaagac
      421 ggtgtgattt atacacatcc gattaacaac tatattctta atggtattac acgaattgta
      481 attaaaaaaa ttgccgaaga ctataacatc ccatttaaag aagaaacgtt tactgtagat
      541 ttcttgaaaa acgcagatga agttattgtt tcaagtactt cagctgaggt tacacctgtt
      601 attaaattag atggtgaacc agttaatgat ggtaaagttg gcccaattac acgtcaacta
      661 caagaaggat ttgaaaagta tatagagtca cacagtattt aaaattcttt catcatattt
      721 ttagattaaa ttacttaaat ttatcaactt tcgtaagtga attatgttat aatttaaaac
      781 gaagcttaaa agtgttgttt agaaaacaca tattagtaaa aagtgccatt ttgtttctca
      841 ttttcagttg aaatataatg ataaaaatta taaaattttc tatgagttac aaatatgatg
      901 tgcatcatat tttactaagt acgtaaatgt tacagttcat aaatttatgc taaccatgaa
      961 aatgggatag caattatttt gaaaactaga gtttatcgcc tacagagatt caaaaacatg
     1021 tagttttaga cacggtaaca ttatatgtca agtaagtgac tatagaatgt atggtgtcaa
     1081 tgacagacat tacgatttaa atttagtcgt gtaatgtagg cattggttga ggatttacaa
     1141 taattatctg gacataatat ttgtaccagt atggtgttat aaattagaat atatggctct
     1201 tgaaaaacct atttctagag ccattttcta attgaaagtg aggtggacga gttggagcag
     1261 ttttatcaat tagggtggac acttgattca gcaggtggtg catctggtga agcatatatg
     1321 gctgaacaag atggacaaaa gttgttttta aaacgaaatt caaatccatt tattgcggca
     1381 ttatcagcag aaggtattgt gcccaaatta gtatggacga aacgcataga aacaggcgag
     1441 gttgttacag cacaacattg gaaaaatggg cgtgaactat cttcaaacga aatgaagcaa
     1501 acaagagttg cacatttatt aaagaagata cacaattcta gacctttatt aagtatgtta
     1561 aagcgtatgg aaatggaacc tattactcct gagattatgc ttaataaaat taatgcctct
     1621 ttatcaagag aagttttaac acatcatatt gtgagaaaat cattaaccta tttagaagag
     1681 catataccga gtttagattc gcgttttttc actgttgtac atggtgatgt gaatcataat
     1741 aattggttat tatccgatcg agatgaacta tttttagttg attgggaagg tgcaatgatt
     1801 gcagacccgg caattgatat cggaatgctg ctttacaact atgttccaca acaacaatgg
     1861 tctgaatggc ttgaaacata tggtgttcaa gagagcttga atttaaataa acgtatgaaa
     1921 tggtatacgg taattcaatc tataggtctt gttcaatggt atgaagagca aaaaagatat
     1981 aaggatatga acacttggct taaattccta aatgaagtta tgaatagtaa catgtttatt
     2041 taagaggaga ataaaatatg agagttcgat acaaaccatg ggctgaagat tatttaaaag
     2101 atcatcctga attggttgac atggacggtc aacatgcagg gaaaatgact gaatggtttg
     2161 ataaaacgca accgatacac attgaaattg gttcgggtat ggggcagttt attacaacat
     2221 tagctgctca aaatcctcat attaactata tttcaatgga acgtgagaaa agtatcgttt
     2281 ataaagtatt ggataaagtt aaagagatgg gtttaacaaa tttaaagatt atttgtaatg
     2341 acgccattga attaaatgaa tattttaaag acggcgaagt ttcgcgtatt tatttgaact
     2401 tttcagatcc atggccaaaa aatcgtcatg caaaacgacg tttaacgtat catacgtttt
     2461 tagcgttgta tcaacaaatc ttaaatgatg aaggagattt acattttaaa actgataaca
     2521 gaggactatt tgcctacagt ttagaaagta tgtctcaatt tggaatgtat tttacaaaaa
     2581 taaatctaaa tttacatcaa gaagacgatg gcagtaatat tttaacagaa tatgaaaaga
     2641 aattttcgga taaagggtca cgtatttatc gtatggaagc gaaattccat tcacaaaaat
     2701 agagcactta tgagggtatg tacacataca atcataagtg ttttttcgta ctataatggt
     2761 tctatagata aaggttctat aataaatcgg actgatggaa aaagttttta acttttcatc
     2821 tgtccgattt tttg
//
LOCUS       DANRDI010000238         2830 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.241, whole genome shotgun sequence.
ACCESSION   DANRDI010000238 DANRDI010000000
VERSION     DANRDI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.241"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..552
                     /locus_tag="PZ377_002713"
     CDS             <1..552
                     /locus_tag="PZ377_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDM6359560.1"
                     /translation="EKIGNSFFQSVTTRTAGFNSIDIASINKSTALMLMLLMFIGGAP
                     LSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKHIKLSIVTINISFLFISII
                     TFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGITKIIIIFVMLCGKVGLLT
                     LLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(1405..1848)
                     /locus_tag="PZ377_002714"
     CDS             complement(1405..1848)
                     /locus_tag="PZ377_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6359561.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(2013..2390)
                     /locus_tag="PZ377_002715"
     CDS             complement(2013..2390)
                     /locus_tag="PZ377_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359562.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            complement(2708..>2830)
                     /locus_tag="PZ377_002716"
     CDS             complement(2708..>2830)
                     /locus_tag="PZ377_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDM6359563.1"
                     /translation="WDGGDEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
BASE COUNT         1023 a          503 c          294 g         1010 t
ORIGIN      
        1 gaaaaaatcg gaaattcttt tttccaatca gtaacaacac gaacagcggg ttttaacagt
       61 atagatatag caagcattaa caaatctacc gcattaatgt taatgctact tatgtttatt
      121 ggtggtgccc ctctcagtgc agctggagga attaaaataa ctacttttgc agttgcgttt
      181 atttttgtac taaattatat acgtaaagaa aataatgttt cagtattcaa taaagaaata
      241 tctgacaaac atataaaact atctattgtt accattaata tctcatttct atttatcagc
      301 atcattactt ttatattatc gataattaat ccgaacatat cattaatcaa gttattattc
      361 gaagtggttt ctgcattcgg aacagtaggg ttaagtatga accttaccac agaatatcat
      421 ggtattacta aaataattat tatattcgtt atgctttgcg gtaaagtagg actattaact
      481 ttattaagaa catttatacc accaaaaagt cctaaaaatt accgctacac taaaggacaa
      541 atttatctat aaaataacta tccatcttta ataagaaatg aactagtgtt ttaatctatt
      601 acattaaaaa aactacctaa acaggtagtt ttttatttat tttaggccga ttaaatgtct
      661 tatgtcttta attaagtaaa atgcacaagt acacctttaa aaagatttac tcaaaactaa
      721 actataccta gttaaccgct tgtgattaag aaattttaaa tgtggtttct tttaattatg
      781 tcaaccaccc ataaggattt taatcaagca ctcattcatt cctgcactcg caatatgcat
      841 tacttataga agtggatgca ctctcatatt atggcaatac cataactccc gcaccataat
      901 gatttaaata ccattatgaa tctcaattac aaattagcac ataaaaaaga cgcctcccat
      961 aagtctagtc accaaatgga aaacgtctcg taattaaaat atatttgcgt gcattctaat
     1021 ttgtacttag aatacatcat actcgttcat aatgtaattt tctaattagt caaaactata
     1081 tacagtttta catcattccc ggcattatgc actaataaaa acttacttta atacacgctt
     1141 taaagattat taaatcagcg ttttaatttt tagacaacgc tattattttt agtttttcaa
     1201 ttctattatg tcataattat gtcactcaaa aactgttttc caacattgat ttattgttgg
     1261 aaaatctcga aaatctagtc tatttctcca acaatcgaag aattatgccc ttttttctcc
     1321 ctttaaataa gtcataatac gaggcataca tgcaacattt acaataaaat atgttcctac
     1381 atcgtattat acgaatgctc acacttatag ttgtttagta aacccagatg tattgctaac
     1441 atacccatta tcactatata gtttatgtgc agatagtctt tcatttctat taccactatt
     1501 tagtgttatt gctttacaat tcaaccgttt agaaaattct tcagaatcag ctaataatct
     1561 ctttccataa ccttttttcc taaattcaga atgtataaca aacgcaagga ttctcatata
     1621 ctctgcattt ttttcgtaaa acatcatttt acacatacca cttaaaccaa ttattttatt
     1681 ttcttttatc aaaagcagta ggaagtaatc atcatgattc gttattttct ttagtctctt
     1741 ttttaaatca ttctcatttg ttggataacc tagatcatca tataatttac atagttgatt
     1801 taatttttca aaatcactgt tattaaacaa tcttgtaatt atgctcatat aacccaccct
     1861 aaaaatatta tgtacctatt gttacataca atagatacgc aaaatcattt ctcactgcaa
     1921 atcaggacgt ttctcagcgt aaacaaatca tttctattct tttgaaaaat atctgcatat
     1981 cttcacagta attttataac agattaataa aattaaaaaa acataacaac aagtattata
     2041 ataaatatta taatagctat cgtattaaat agtttagaat atgcaaactg aaaaataaaa
     2101 atcacaatta aaatactaag taatgtaaat ggtaatagca tcatcagatc tttaacatta
     2161 tttctttgtt cttgctccca atcgctaata gccctaaaac tacttttttt atctataggc
     2221 gatgtaaaaa taccatatat tgaaggtgct atacctccta aaatagcagt tcccaaagtt
     2281 gtcattactg aaattactgc gaaagtatca tccgaaagca ataaattcaa actaatgcat
     2341 tgtttattac ccatcgaatt tattgaccaa atagctagag aaataaacaa cccaaaattt
     2401 aaaataaatg atatagtaat agcaattgtt tacaaaacac gaaatttttc atttttattt
     2461 atattatcca tttttctccc tttttcttaa atcattttat tatatattac aataatcaat
     2521 ctgaaatgtt gatgtaattt gaaaaaaata tcatactttt actcctgaaa acctccctaa
     2581 atcatcaata tgaaaatcag tattaggata ttgcgtttac aactctttta aatctcactc
     2641 attcttctca tcgtcttaac cgatactatc aataaatact ggcaacacca taaaatatat
     2701 acccaattta taaaaaccta attgactcat aatcttctaa cggtatatca tccacaatca
     2761 cagtatgatt aggattatcg ttagatacat ctttcactgc cttatctaac ccctcatcac
     2821 ctccgtccca
//
LOCUS       DANRDI010000239         2819 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.242, whole genome shotgun sequence.
ACCESSION   DANRDI010000239 DANRDI010000000
VERSION     DANRDI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.242"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            437..883
                     /locus_tag="PZ377_002717"
     CDS             437..883
                     /locus_tag="PZ377_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDM6359564.1"
                     /translation="MKKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISR
                     DLQELEIYKIPVENKKYIYKKINQTNQLSAKKQLEIFSDEIIEFITLNNYVLIKTSPG
                     FAQSISYYIDQLQMKEILGIIGGNDTLMILTSSNEIAEFVCYQLFP"
     gene            1152..2387
                     /gene="arcA"
                     /locus_tag="PZ377_002718"
     CDS             1152..2387
                     /gene="arcA"
                     /locus_tag="PZ377_002718"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDM6359565.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            2473..>2819
                     /locus_tag="PZ377_002719"
     CDS             2473..>2819
                     /locus_tag="PZ377_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6359566.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFP"
BASE COUNT         1061 a          378 c          452 g          928 t
ORIGIN      
        1 gcttattctc atattcaaat tattcaaata tattccttat catgtattag attctatttt
       61 ttatattatt tttgtaattc tttgtataaa cctatttatt tataaagcgc tctaatacat
      121 acctaaatat cttcaactta taaactactt tttatttcaa tgcctttttc caaacttctt
      181 ttatgagata ttgtggttat tacctgacat ttacaattta cacatttcgc tattattacg
      241 caaattattt tgattttata attatgatga ctataatcta ttctaattaa tctctccttt
      301 taggcataaa ataatctact tataattaaa gcatccacta tttcaaaatt atttgtaaat
      361 tgagatttat aagtgatttt tatacagtta tttaaaatct atttcataat caaaaataat
      421 aaagggggta ataaaaatga aaaaagaaaa acgtttaaat cttatcttaa ctgttataca
      481 acagaatcag tttaataaaa aacaacaaat agtggattac atggcaagac attttggagt
      541 ttactatagc ttgacaacta tttctcgtga cttacaagaa ttagaaattt acaaaatccc
      601 tgttgaaaat aaaaagtata tttacaagaa aataaatcaa acaaatcaat taagtgcaaa
      661 aaaacaatta gaaatattta gtgatgagat tattgaattt ataacgctaa ataactatgt
      721 cttaataaaa acatctcctg gctttgctca aagtataagt tattacatag atcaattaca
      781 aatgaaagaa atattaggaa ttattggagg taacgatact ttgatgattt tgacttcttc
      841 aaatgaaata gcagaatttg tttgttatca attattccct taaaaattaa atgattatat
      901 taataggaat gattactaat acaaaaaaat gagtaaaaaa tctttttgct tgaataagat
      961 aaggttttaa caatgagatt tttcaaatta ttcactatta tatagtacaa aatacatatt
     1021 catacaaaat tttgcattga ataaatctat atatgtgaaa accctcccta aacaattgtg
     1081 aattaaatca cacgatgttt tttgaaagcg ttttataatg tgaatgtaat aaagcaaagg
     1141 aggaatttga aatggtacaa ggacccattc aagtaaatag tgaaataggc aaattgaaaa
     1201 ctgtgttgtt aaaaagacca ggaaaagaat tagaaaattt agtacctgat catttaagtg
     1261 gtttattatt cgatgatatt ccctacttaa aagttgcaca agaagagcat gacaaatttg
     1321 ctcaaacttt gagagatgaa ggaatcgaag tagtttattt agaaaaactt gcagcagaat
     1381 ctattactga gccagaagta cgcgagaact tcataaacga catattaaca gaatctaaaa
     1441 agacaatatt aggtcatgaa actgaaatta aagaattctt ttcaaagtta tctgaccaag
     1501 aacttgtaaa taaaatcatg gctggcgtac gtaaagaaga aattcaactt gaaacaaccc
     1561 atttagtaga atatatggat gatagatatc cattttactt agatccaatg cccaaccttt
     1621 attttacaag agatccccaa gcttcaattg gtagaggaat gacaattaac agaatgtatt
     1681 ggagagcacg acgtagagaa tctattttta tgacatatat actgaaacat catccaagat
     1741 ttaaagataa agatgtacca gtatggttag atcgtaactc accatttaat attgaaggtg
     1801 gagatgaatt agtattatcg aaagatgttt tagctattgg tatatcagaa cgtacatcag
     1861 ctcaagcaat agaaaagtta gcacgtaata ttttcaaaga tgcaaacaca agttttaaaa
     1921 aaatcgtagc tattgaaata cctaatacac gtacatttat gcacctagat acagtactaa
     1981 ctatgattga ctacgataag tttacagtac atgcagcaat atttaaagaa gaaaataata
     2041 tgaatatatt taccatagaa caaaatgatg gtaaggacga tataaaaatt actcgttcta
     2101 gcaagttacg tgaaacactt gctgaagttt tagaagtaga aaaagtggac tttattccaa
     2161 caggtaatgg cgacgttatt gatggtgcac gtgaacaatg gaatgatggc tcaaacacat
     2221 tatgtattcg accaggggtt gtggtgacat acgatcgcaa ctatgtatca aaccaacttt
     2281 tacgcgacaa aggaattaaa gtgattgaaa ttactggtag tgaacttgta cgtggacgcg
     2341 gaggcccaag atgtatgagt cagccgttat ttagagaaga tatttaaatt tgaaaggtgg
     2401 taaaagtcac aagtaattca ttcattaaat caaataacaa gatttttacc acctttatta
     2461 ggggagatta atatggatga aaataaatta ggtaaaactt ccttaattgg tttagtcata
     2521 ggctctatga taggcggtgg tgcattcaat atcatctcag atatgggtgg ccaagctggt
     2581 ggacttgcaa taattatcgg ttggataata actgctattg gtatgatttc tcttgctttc
     2641 gtatttcaaa atttaacaaa tgagcgacca gatcttgatg gaggaattta tagttatgct
     2701 caaacagggt ttggagattt tattggtttt tcaagtgctt ggggatattg gtttgcagca
     2761 tttctaggta atgtggctta tgcaacccta ttaatgtcag ctgtgggtaa ctttttccc
//
LOCUS       DANRDI010000240         2759 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.243, whole genome shotgun sequence.
ACCESSION   DANRDI010000240 DANRDI010000000
VERSION     DANRDI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.243"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(103..354)
                     /locus_tag="PZ377_002720"
     CDS             complement(103..354)
                     /locus_tag="PZ377_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDM6359567.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            700..2523
                     /gene="lepA"
                     /locus_tag="PZ377_002721"
     CDS             700..2523
                     /gene="lepA"
                     /locus_tag="PZ377_002721"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDM6359568.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
BASE COUNT          987 a          368 c          530 g          874 t
ORIGIN      
        1 taaatcatca tatatggata aacagttaat actggaatta ttcattctat ctttataaaa
       61 aataaaagtt caagcatgcg cttgaacttt tatttaaaaa gattatttat ttgcagtcat
      121 taattgtgat ttaatacggt cagctttgtt tgaatgtatt aaattacttt gagcagcttt
      181 gtctactaac ttaactgcta agcttactaa ttcattttta ttatcagcgt tatttgaaac
      241 agctgtttta gcgtttttaa ctgctgtacg cattgcactc ttttgtgaaa tgttgcgtgc
      301 ttcagctttt tcagttgttt ttacacgttt aattgcagat ttgatatttg ccatttctgt
      361 cacctcctaa aagtgagttc atcttatcaa aattttattt gattacaaca agaaatattt
      421 taacaaaagt ctaaccgttg tgcaatcatt tatttgatgg ggttagctta aaattttgca
      481 gatttaataa attgtgttgg aaacacaatc aatacgtttt ataattataa gtacgtaatt
      541 aacatattaa catatcgcaa gtatgtattt aaataaagat tgttataatt tcaaagttca
      601 tccaagatta tggcgtttgc atttacctat taaaaacgtt attatatcaa agatgcgaaa
      661 gataatacgg gtttatttta tgaaagtgag aaggataaaa tggataatga gcaacgctta
      721 aaaagaagag agaatataag gaatttctcg attatagcac atattgacca cggaaaatct
      781 acattggctg atagaatttt agaaaatacc aaatcagttg aaacaagaga tatgcaagat
      841 cagttactag attcaatgga tttagaaaga gaacgtggta ttacaatcaa attaaacgca
      901 gtacgtttaa agtacgaagc taaagatgga aatacttata cattccattt aatcgatacg
      961 cctggacacg tcgattttac atatgaagtg tcacgttctt tggcagcttg tgagggcgcg
     1021 attttagtag tagatgcggc tcaaggtatc gaagcacaaa cattagcaaa tgtttattta
     1081 gcattagata atgagttaga gttattgcct gttattaaca aaattgattt acctgctgca
     1141 gaacctgaac gcgtgaaaca agaaattgaa gatatgatag gtttagacca agacgatgtt
     1201 gttttagcaa gtgctaaatc taacattgga attgaagaga tactagagaa aatagttgaa
     1261 gttgtgccag ctccagatgg tgacccagaa gcaccactaa aagcgttaat atttgattct
     1321 gagtatgatc catatagagg ggtaatttca tcgataagaa ttgtggacgg tgttgttaaa
     1381 gccggagata aaattcgaat gatggccact ggtaaagagt tcgaagtaac agaagttgga
     1441 attaatacac ctaagcagct tccagttgat gaattaacag ttggtgatgt tggttatatt
     1501 attgcaagta ttaaaaatgt tgatgattct agggttggtg acaccatcac attagctagt
     1561 agacctgcat cagaaccatt gcaaggttat aagaaaatga atccaatggt atattgcgga
     1621 ctgttcccaa tagataacaa aaattataat gatttaagag aagcattaga aaaattacaa
     1681 ttgaatgatg catcattaga atttgagcct gaatcgtcac aagcattagg ttttggttat
     1741 agaactggtt tcttaggtat gttacacatg gaaataattc aagaaagaat tgaaagagaa
     1801 tttggtattg aattaattgc aactgcacca tctgtaattt atcaatgtgt tttaagggac
     1861 ggttcagaag tgacggttga taacccagca caaatgccag atcgtgataa aattgataaa
     1921 atatttgagc catatgttcg tgcaactatg atggttccaa atgactatgt cggtgcagta
     1981 atggaattat gtcaacgtaa acgtggacaa tttataaata tggactattt agatgatatt
     2041 cgtgtaaata ttgtttatga attaccttta gctgaagttg tatttgattt cttcgatcaa
     2101 cttaaatcta atactaaagg atatgcatca tttgattatg aattcatcga aaataaagaa
     2161 agtaatttag tcaagatgga tattttatta aatggtgata aagtggatgc gctaagcttc
     2221 atagttcata gagattttgc atatgaacgt ggtaaagcat tagttgaaaa acttaaaacg
     2281 ttaattccaa gacagcaatt tgaagtacct gtacaggctg caataggaca aaaaattgta
     2341 gcgcgtacaa atattaaatc aatgggtaaa aacgttttag ctaaatgtta tggcggtgac
     2401 ataagccgta aacgtaaatt acttgaaaaa caaaaagcag gtaaagctaa gatgaaagca
     2461 gttggtaatg ttgaaattcc acaagatgct ttcttggctg tattgaaaat ggatgatgaa
     2521 taattttaaa aaatcaatta acaatttaca atgaataaag tttaataact aaaaagaggg
     2581 agcctaggat aaattaacgt cctgggcttt acaatgttat attggcagcc atcgacagag
     2641 ttaaaatgag cttataacaa tggggcccca acacagaagc tgacgaaaag tcagcttact
     2701 ataatgtgca agttggggtg gggccccaac atagagaatt tcgaaaagaa attctacag
//
LOCUS       DANRDI010000241         2748 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.244, whole genome shotgun sequence.
ACCESSION   DANRDI010000241 DANRDI010000000
VERSION     DANRDI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.244"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..636
                     /locus_tag="PZ377_002722"
     CDS             <1..636
                     /locus_tag="PZ377_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDM6359569.1"
                     /translation="LELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIEKYLKE
                     RNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRMWQGAS
                     GKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWNLKYKQ
                     LKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL"
     gene            1019..1691
                     /locus_tag="PZ377_002723"
                     /pseudo
     CDS             1019..1691
                     /locus_tag="PZ377_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
     gene            1713..>2748
                     /locus_tag="PZ377_002724"
     CDS             1713..>2748
                     /locus_tag="PZ377_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDM6359570.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEI"
BASE COUNT         1160 a          307 c          482 g          799 t
ORIGIN      
        1 cttgaattac ttcaacaaca gaaaaaaggc tatatgcaga aaattttctc acaggaactg
       61 cgattcaaag atgagaatgg tgaagattat ccagattggg aaaatagcaa aatagaaaaa
      121 tatttaaaag agagaaacga acgttctgac aaagggcaaa tgctttcagt aactataaat
      181 agtggcatta taaaatttag tgaattggat agaaaagata attcaagtaa agataaaagt
      241 aattataaag tagttaggaa aaatgatatt gcatataatt ctatgagaat gtggcaaggg
      301 gctagtggta aatcaaatta taatgggatt gttagccctg catatactgt gctttatcca
      361 acacaaaata ctagctcatt atttattgga tataagttta aaacacatag aatgattcat
      421 aaatttaaaa ttaattcaca aggattaaca tcagatacat ggaacttaaa atataaacaa
      481 ttaaaaaata taaatataga tatacctgta ttggaggaac aagaaaagat aggtgatttc
      541 tttaaaaaaa tggatatatt gataagtaaa cagaaaatga aaattgaaat attagaaaaa
      601 gagaaacaat cctttttaca aaaaatgttc ttataacttt gataaataca tagattgcat
      661 aagaataaaa tttgtataat ttaacataaa agttgtaaaa gtaaagtgaa ttaaaaacga
      721 acattaaatt taggcactgt gaaagcgcag tgtctttttt gtgtcgaaat tgtgtacaga
      781 ataagtagtt aaataaagat taagttgaga taaagtgtta ttcgtaaata aaagagagta
      841 gatcgatagg aattgaatga tattagttaa ctatttatta aattacttaa taatgattaa
      901 tttttagtta aagtaagttt aatgtgaagc acgaccattg ctcattataa tgaatgagga
      961 ttgttcgtat tgcgtaatag aataaatcaa atagactaaa aattgggagc atagaattat
     1021 gaaattaaaa aatattgcta aagcaagttt agcactaggg attttaacaa cagggatgat
     1081 tacaactact gctcagccag taaaagcaag tacattagag gttagatcac aagctactca
     1141 agacttgagt gaatattata atagaccgtt ctttgagtat acaaatcagt caggatataa
     1201 agaggaagga aaagtgacgt ttactcctaa ttatcaactt atagatgtaa ctttaactgg
     1261 gaatgaaaag caaaattttg gtgaagatat ttctaatgta gatatatttg ttgtaagaga
     1321 aaattctgat agatctggta acttcaattg gtggtattac taaaacaaac ggttcaaatt
     1381 atattgataa agtaaaagat gtaaatttaa taattactaa aaacatcgat agtgttacat
     1441 caacgtcaac atcatctaca tatacaatta ataaagaaga aatttcatta aaagaacttg
     1501 attttaaatt aagaaagcat ttaattgata aacataacct ttataagaca gaacctaaag
     1561 acagtaaaat tcgaattact atgaaagatg gtgggttcta cacatttgaa ttgaataaaa
     1621 agttacaaac acaccgtatg ggtgatgtta ttgatggcag aaatatagaa aaaattgaag
     1681 tgaatttata aaattattcg agggagcata tcatgaggga aaattttaag ttacgtaaaa
     1741 tgaaagtcgg tttagtatct gttgcaatta caatgttata tattatgaca aacggacaag
     1801 cagaagcatc tgaaaatcaa aacgctttaa tctctaatat aaatgtagac aatcaggaaa
     1861 aacagaataa tgtaaatcaa gctgttcagc ctcaaaataa tactaatgaa acatcaaaag
     1921 taccggctaa ttttgtcaaa ttgaatgata ttaaaccagg tgatacttct atacaaggaa
     1981 caactttacc aaatcaattt atactattaa ctattgataa aaaagatgtg agctcagttg
     2041 aagattctga cagcagcttt gttatgtctg ataaagatgg gaattttaag tatgacttaa
     2101 atggtcgcaa aattgttcat aatcaagaaa ttgaagtgtc ttcatcagat ccctatttag
     2161 gtgacgatga agaagatgaa gaagtagaag aaacttcaac tgaagaagtt ggtgctgagg
     2221 aagaaagtac agaagctaaa gctacatata caacaccgcg atatgaaaaa gcgtatgaaa
     2281 taccgaaaga acagctaaaa gaaaaagatg gacatcacca agtttttatc gaacctatta
     2341 ctgaaggttc aggtattatt aaaggccata cctctgtaaa aggtaaagtt gctctatcta
     2401 ttaataataa atttattaac tttgagacaa atgctaatgg tggtccaaat aaagaagaag
     2461 cgaaatctgg atcagaagga atctggatgc ctattgatga caaaggatac tttaattttg
     2521 acttcaaaac gaaacgtttc gatgatttag agttaaagaa aaatgatgag atctcattaa
     2581 catttgcacc tgatgacgaa gatgaggcat tgaagtcatt aattttcaaa actaaggtaa
     2641 cgagtttaga agatattgat aaagcagaaa ctaaatatga ccatactaaa gtggaaaaag
     2701 taaaagtatt gaaagatgtt aaagaagatt tacatgtaga tgaaattt
//
LOCUS       DANRDI010000242         2707 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.246, whole genome shotgun sequence.
ACCESSION   DANRDI010000242 DANRDI010000000
VERSION     DANRDI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.246"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1510
                     /locus_tag="PZ377_002725"
     CDS             <1..1510
                     /locus_tag="PZ377_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDM6359571.1"
                     /translation="RIANHRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNV
                     NRSYILEAIKDSLKRLIVPSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQ
                     ILGVDPAFRTGCKLAVINPFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIA
                     IGNGTASRETEQFVADLIKKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSA
                     VSIGRRVQDPLSELVKIDPKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTA
                     SSSLLQYVSGLSSQIAKNIIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDG
                     SEPLDNTSIHPESYKVTYQLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPT
                     LEDIIKSLKAPNRDPRDEFETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGV
                     KQDGLVHVSKLSKKFVKNPMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            1503..1958
                     /locus_tag="PZ377_002726"
     CDS             1503..1958
                     /locus_tag="PZ377_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDM6359572.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            2605..2687
                     /locus_tag="PZ377_002727"
     tRNA            2605..2687
                     /locus_tag="PZ377_002727"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2638..2640,aa:Leu,seq:gag)
BASE COUNT          990 a          363 c          519 g          835 t
ORIGIN      
        1 acgcattgct aatcatagag ttttagctgt taatcgtggt gaaaaagaga aagtattatc
       61 tgtaaagttt gaattcgata cgacatcagt agaggatttc attgcacgtc aagaaatcaa
      121 tcataataat gtaaatcgca gttatatttt agaggcgatt aaagatagct tgaaacgctt
      181 aattgtccct tcgatagagc gtgaaatcca tgctgattta actgaaaaag ctgaaaatca
      241 tgcaatagat gtttttagtg aaaacttaag aaatctatta ctgcaacctc caatgaaagg
      301 taaacaaata ttaggcgtag atccagcatt tagaacaggt tgtaaattag cagtcattaa
      361 cccattcggt acttttatag caaaaggtgt gatttatccg catccaccag tttctaaaaa
      421 agaggcagca gagaaggatt ttgtacaaat ggttaaagcg tatgatgtgc aattaattgc
      481 aattggcaat ggtactgcaa gtcgtgaaac agaacaattt gttgcagatt taattaaaaa
      541 gcatcagttg ccagtacaat tcatcattgt caatgaagcg ggcgcttcag tatactcagc
      601 atcagaaatt gctagagatg aatttcctga ttttcaagtg gaagaaagaa gtgccgtatc
      661 aattggacga cgcgttcaag atccattaag tgaattagtt aaaatcgatc ctaaatctat
      721 tggtgtcgga caataccagc atgatgttaa tcaaaaagca ctagaaaatg cattaacttt
      781 cgttgttgaa acagcagtta accaagtagg tgttgatgtc aatacagcat catcatcatt
      841 attacaatat gtgtctggtc taagttcgca aattgctaaa aatataattg catatagaga
      901 agaaaatggc gcaataaagc ataataaaga gttaagcaag attaagcgtt tgggtgctaa
      961 aacttttgag cagagtatag gatttttaag aatagtagac ggttcagagc cattggataa
     1021 tacatcgatt catcccgaaa gctataaagt aacgtatcag ttattagata aactcggatt
     1081 tggtggcaat gatttaggta gtgatgcatt aaaagctaaa ttaaattcat tggatatgga
     1141 tgaactggcg attgaattac aagtcggtgt acctacatta gaggatatta ttaaatcctt
     1201 gaaagcacct aatcgagatc caagggacga gtttgaaaca ccaatcctta aatccgatgt
     1261 gctatcaatt gaagatttac aagaaggaat gaaattgagt ggtacagtta gaaatgtagt
     1321 agattttgga gcatttgtag atataggtgt taaacaagat ggacttgtcc atgtttcgaa
     1381 actttctaag aagttcgtaa aaaatccaat ggatattgtc agtgttggtg atatcgttga
     1441 cgtttggatt tacagtattg acaaaaataa agacaaagta tcattaacga tgattgatcc
     1501 acatgaataa tgacaaactt cagcgaatgg ttgagaatct ttcggaagaa aaatttggac
     1561 gtacgtttcg acattgtgca tattttaata aaaggttacg tacaacaggc ggacgttatc
     1621 ttttaaagtc ccatgatatt gaaattaatc caaaacaata tgagcattat ggagaagatg
     1681 ctgttgtaaa aattatactg cacgaattgt gtcattatca cttgcatatt gctggtaaag
     1741 gttatcaaca taaagatcaa gattttaaac gattgagcca acaggttgga gcgcctagat
     1801 tttgtaacag cattgaaagc tatcaacaac gggcaaatta tgaatattat tgcactaaat
     1861 gtcatgcaaa atatatacgt atccgtaaag ttgatacgaa tcgtatgcgt tgcggacatt
     1921 gcaatggaaa attaagaatg aaaagacaat tgaaataagt acaatacaaa tagagctaat
     1981 tcgtgtatat gtaagattat ttatgagttg tatggaattt atatagaatt tatcagtgag
     2041 ttattttgat tggtaaacat agtaattgaa taagaagaaa ttgtgtttat aaaatgttta
     2101 aagccgagat tgtcgtaatt taatacttcg caatctcggc ttttctactt atgatatagg
     2161 taatggcata aattgtggct tgataggatg aaattaaaat gattagatta aaaatcttgg
     2221 cgtattttag taataattaa tatataaact ttacccaatt aaagctttaa agtagcacaa
     2281 aagataatta tacatttttc atgacatttt gattgaactt ttctattgac tgtacagctt
     2341 atagttagta tgattaaaca cgttccttaa aaaagaacaa attaaataaa ctttgatttg
     2401 aaagacagtc aattctaaat ttaatttata aaatatgttg acaataagtt ctaaatgttt
     2461 tattataaat agcgttgtgt taacaactga taacacaaat taatttaatg taaaaaaacg
     2521 attaaaagtt attgacaaat atttttaaac ataatataat aaataacgtt ctgaaaaacg
     2581 aagatgtttt aaatgcctat atccgcggtc gtggcggaat ggcagacgcg ctaggttgag
     2641 ggcctagtgg gagaaatccc gtggaggttc aagtcctctc ggccgcatca aaattcttaa
     2701 tttaaat
//
LOCUS       DANRDI010000243         2707 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.247, whole genome shotgun sequence.
ACCESSION   DANRDI010000243 DANRDI010000000
VERSION     DANRDI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.247"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..586
                     /locus_tag="PZ377_002728"
     CDS             <1..586
                     /locus_tag="PZ377_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDM6359573.1"
                     /translation="RHYTKGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNK
                     EQVLFPISNLFHPKEVNELCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNE
                     QQHMNYNMALTSKFAKDRIIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRE
                     TAGHIIHELKDEKLVVKDHKNWLVSKHLFNDVCV"
     gene            936..>2707
                     /gene="clfB"
                     /locus_tag="PZ377_002729"
     CDS             936..>2707
                     /gene="clfB"
                     /locus_tag="PZ377_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDM6359574.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDS"
BASE COUNT         1047 a          410 c          482 g          768 t
ORIGIN      
        1 cagacattat acgaaaggcc aagttattta tttttcgcca caagaaagta gcaatattta
       61 ctttttaatt gaaggtaaca ttattagaga acattacaat caaaatggag atgtatatcg
      121 ttattttaat aaagagcaag tattatttcc aatcagtaac ttatttcatc cgaaagaggt
      181 taacgaattg tgtacagcat taaccgattg tacagttctt ggattgccta gagaattgat
      241 ggcctttttg tgcaaagcta atgatgatat atttttgaca ctttttgcat taataaatga
      301 taatgagcag caacacatga actataacat ggcattaaca agtaaatttg ctaaagatcg
      361 aattatcaaa ttgatatgcc atctatgtca gacagtagga tacgatcaag atgaatttta
      421 tgaaatcaaa cagtttttaa ctattcaact catgagtgat atggctggga tttcccggga
      481 aacagctggt catattattc atgaacttaa agatgaaaaa cttgttgtta aagatcataa
      541 aaattggtta gtaagcaaac atttattcaa tgatgtatgt gtttaatata caatgtaaaa
      601 tgaataagtt gaacatgagg tctaacgtac atttatacgt taggcctttt ttgctagcat
      661 gatgaataat ttaaaatgtt agttaaattt gattgttgaa attacagtaa aatttaaggt
      721 gatgaaaaat ttagaacttc taagtttttg aaaagtaaaa aatttgtaat agtgtaaaaa
      781 tagtatattg atttttgcta gttaacagaa aattttaagt tatataaata ggaagaaaac
      841 aaattttacg taattttttt cgaaaagcaa ttgatataat tcttatttca ttatacaatt
      901 tagactaatc tagaaattga aatggagtaa tatttttgaa aaaaagaatt gattatttgt
      961 cgaataagca gaataagtat tcgattagac gttttacagt aggtaccaca tcagtaatag
     1021 taggggcaac tatactattt gggataggca atcatcaagc acaagcttca gaacaatcga
     1081 acgatacaac gcaatcttcg aaaaataatg caagtgcaga ttccgaaaaa aacaatatga
     1141 tagaaacacc tcaattaaat acaacggcta atgatacatc tgatattagt gcaaacacaa
     1201 acagtgcgaa tgtagatagc acaacaaaac caatgtctac acaaacgagc aataccacta
     1261 caacagagcc agcttcaaca aatgaaacac ctcaaccgac ggcaattaaa aatcaagcaa
     1321 ctgctgcaaa aatgcaagat caaactgttc ctcaagaagc aaattctcaa gtagataata
     1381 aaacaacgaa tgatgctaat agcatagcaa caaacagtga gcttaaaaat tctcaaacat
     1441 tagatttacc acaatcatca ccacaaacga tttccaatgc gcaaggaact agtaaaccaa
     1501 gtgttagaac gagagctgta cgtagtttag ctgttgctga accggtagta aatgctgctg
     1561 atgctaaagg tacaaatgta aatgataaag ttacggcaag taatttcaag ttagaaaaga
     1621 ctacatttga ccctaatcaa agtggtaaca catttatggc ggcaaatttt acagtgacag
     1681 ataaagtgaa atcaggggat tattttacag cgaagttacc agatagttta actggtaatg
     1741 gagacgtgga ttattctaat tcaaataata cgatgccaat tgcagacatt aaaagtacga
     1801 atggcgatgt tgtagctaaa gcaacatatg atatcttgac taagacgtat acatttgtct
     1861 ttacagatta tgtaaataat aaagaaaata ttaacggaca attttcatta cctttattta
     1921 cagaccgagc aaaggcacct aaatcaggaa catatgatgc gaatattaat attgcggatg
     1981 aaatgtttaa taataaaatt acttataact atagttcgcc aattgcagga attgataaac
     2041 caaatggcgc gaacatttct tctcaaatta ttggtgtaga tacagcttca ggtcaaaaca
     2101 catacaagca aacagtattt gttaacccta agcaacgagt tttaggtaat acgtgggtgt
     2161 atattaaagg ctaccaagat aaaatcgaag aaagtagcgg taaagtaagt gctacagata
     2221 caaaactgag aatttttgaa gtgaatgata tatctaaatt atcagatagc tactatgcag
     2281 atccaaatga ctctaacctt aaagaagtaa cagaccaatt taaaaataga atctattatg
     2341 agcatccaaa tgtagctagt attaaatttg gtgatattac taaaacatat gtagtattag
     2401 tagaagggca ttacgacaat acaggtaaga acttaaaaac tcaggttatt caagaaaatg
     2461 ttgatcctgt aacaaataga gactacagta ttttcggttg gaataatgag aatgttgtac
     2521 gttatggtgg tggaagtgct gatggtgatt cagcagtaaa tccgaaagac ccaactccag
     2581 ggccgccggt tgacccagaa ccaagtccag acccagaacc ggaaccaagc ccagacccgg
     2641 atccggattc ggattcagac agtgactcag gctcagacag cgactcaggt tcagatagcg
     2701 actcaga
//
LOCUS       DANRDI010000244         2687 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.248, whole genome shotgun sequence.
ACCESSION   DANRDI010000244 DANRDI010000000
VERSION     DANRDI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.248"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1414)
                     /locus_tag="PZ377_002730"
     CDS             complement(<1..1414)
                     /locus_tag="PZ377_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDM6359575.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVL"
     gene            complement(1404..>2687)
                     /locus_tag="PZ377_002731"
     CDS             complement(1404..>2687)
                     /locus_tag="PZ377_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDM6359576.1"
                     /translation="GLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSM
                     LGVRDLSVIETPPENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEK
                     REQLQMLMPDANIAVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTL
                     IIEDADRFGLSQLYQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGS
                     GFKIAMRDLNIRGAGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEV
                     EVDLNLDAYLPTEYIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARL
                     LDIVEIKVHALHSGITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQ
                     NNAMTITLTKQNQWLDSLKFLVKCIEESMRISDEA"
BASE COUNT          883 a          547 c          372 g          885 t
ORIGIN      
        1 ctaatactgc tgcacataat aattcaatga gtccagtgat tctaccgtgt gttgtcacta
       61 tgtttaacac gcattgaaca acaatcgaca taaataccat acctaaaaca acattgataa
      121 aaaatcgtct catcgcatat aagtggtatt ttctcgtgac agcaatatgg ataatcgtac
      181 cgaatataat taatgataca acagtactaa tgctcgcacc aataatgcca cttaaacgaa
      241 tcaaaatgat attaagtata aatttaataa ccaatcctgc cgtcatacca acaaaaatag
      301 gtctcacagc atgttgcgct tgtaataatg ccatatccat cataattaac gatacacaaa
      361 tgaccgtaat catataaaca cttaacgtta aggttaaatc attcgtctta aaaaacacac
      421 cgttcattaa aggcaataaa ttaattaatc ctattcctgc tgctgtactt attaaaatcg
      481 taatctttaa tgacgcattt gcataacgat tcataagtac ctgattattc attttaattg
      541 cgtcacttaa cagaggaatg agcgcaaaac taaatgttgt agttacaatc aatcccatct
      601 gaataaatga tgcaccacgg tcatagactc ctttttcagt tatggcaaca tcgaatggca
      661 cgcgtatcgc ttgaagtgac ttaataatag taacactatc aatcacttgc cataaaatta
      721 cgattaattg actgatagcg aaaatcaaaa ccgaaagtgc gaactgtttc caaacgatct
      781 ttgcagtgtt atttaccatt ttaaacttaa aaggtcggtg cgctactaaa taaattgaag
      841 aacctaaaaa acctatcgtt gatgctaaaa tagcaattgt tcccgcttca tatatcgtcc
      901 aacctctgtc tacaaaaata acaatagtaa caatgataat acctactcgt ataacttgtt
      961 ctataacctg ggaaatagcc ggaactgtca tattatttgc agattgataa taacctctta
     1021 acacgccaag catacctata aatataaagc ttaaacttgc agcttgaatc attggtgtta
     1081 aatggccatc acccatcata tgtgcaatat tgttcgcaaa cacaaaaata gcaataaata
     1141 ataatatacc aactaattgt atataagcga ctgcttttgc atatgcttcg tcactatgat
     1201 acttccctat attttgtgta attgcactag gaatggcatt catcgataat atcattccta
     1261 atgctacaat tggatacact tgttgatatg catacaaacc tgtatcgcct aatatatttt
     1321 gatatggaat tcgatataca gcactcagaa ttttaatgac aattaatgca gcagttaaca
     1381 cgacaacgcc attaaatgct tctttatgct tcatcactga ttctcatact ttcttcaatg
     1441 cacttaacta aaaacttcaa actatcaagc cattgatttt gtttcgttaa agtaattgtc
     1501 attgcattat tttgaacacc aaccttcatt gttctaccta aaggttgtgt tgctttgaac
     1561 agcacttcgc catcaatatt ttcagtggct tttacagata aatgaatatc aattattttc
     1621 cctttatctt taatcaacgt gatacctgaa tgtaatgcgt gtacttttat ttccactata
     1681 tcaagcaaac gtgctacttc aacaggataa tcattgaaac gatcaattaa ttcatcttta
     1741 atgtcgataa tttgatcaaa tgtttcagtt tttcgtagct ttttataaat ttcaatttta
     1801 gcttgttcat ttgcaatata ttctgttggc aaatatgcat ccaagtttaa atcaacttcg
     1861 acttctggca cctcagattc tggttcctta ataccacgtt tttcatttac agcttcttct
     1921 aacatttgac tgtacaaatc aaatccaact gtatcaataa agccgtgctg ttgtttacct
     1981 aacaaattac cagcaccacg aatgttcaaa tcacgcatcg caatcttaaa tcctgagcct
     2041 aactccgtaa attctttaat cgcttgtaat cgatcttctg cagtctcagt tagtacctta
     2101 tttgctggat gtaagaagta tgcataacca atacgacttg aacgaccaac acgacctctt
     2161 aattgataca actgactcaa tccaaagcga tctgcatctt caatgatcaa agtatttgca
     2221 tttgggacat cgacacctgt ttcaataatc gtcgtcgtta ctaaaatatc atattcatta
     2281 ttgataaaac ttaacatcgt ttcttctaaa tcgcgctctg tcatttgtcc atgagcaact
     2341 gcaatgttag catctggcat taacatctgg agttgttctc gtttttcata aatggattgc
     2401 actttattat aaagataaaa cacttggcca tcacgggata gttctctttc taaagcttct
     2461 ttgataaaac tcatgttctg ttctaataca tatgtttgaa ctgggaaacg attttctggc
     2521 ggcgtttcaa tcactgacaa atcccgcaca cctagcatac tcatatgcaa tgttctaggt
     2581 attggggttg cagtcaatgt tagtacatct acattatgtt ttaatgtttt aatacgctct
     2641 ttatggcgta caccaaatcg ttgttcttca tctacaatca acagccc
//
LOCUS       DANRDI010000245         2628 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.250, whole genome shotgun sequence.
ACCESSION   DANRDI010000245 DANRDI010000000
VERSION     DANRDI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.250"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            226..579
                     /locus_tag="PZ377_002732"
     CDS             226..579
                     /locus_tag="PZ377_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDM6359577.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            647..1042
                     /locus_tag="PZ377_002733"
     CDS             647..1042
                     /locus_tag="PZ377_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDM6359578.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            1302..1871
                     /locus_tag="PZ377_002734"
     CDS             1302..1871
                     /locus_tag="PZ377_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDM6359579.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(1998..2198)
                     /locus_tag="PZ377_002735"
     CDS             complement(1998..2198)
                     /locus_tag="PZ377_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDM6359580.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
BASE COUNT          835 a          361 c          510 g          922 t
ORIGIN      
        1 ggggtgggac gacgaaataa attttgcgaa aatatcattt ctgtcccact ccctaatttg
       61 agctggatat actttcattt gaacccttta ttgctagttt atgaaagtat catgaaagct
      121 ttatgaacat cgcttgagtt gcctttacag tagaaaattt aagttttaca ctttgtgtga
      181 atgatacgtt ttgtattgaa ttaattatag aaaggtacgt tgaagatgtt ttcaattgga
      241 agtgcaattc ttcattttgt cattggtggt atcgctgttg cattagcttc aattattgct
      301 gataaggtag gtggtaagtt aggaggtatt atagctacta tgccggcagt ctttcttgcg
      361 gctattatcg cattagcttt agatcatcgt ggtacgcaat tagtggagat gtcgatgaat
      421 cttagtactg gagcaattgt cggtattctg tcttgtatat taactgtatt tttgacatct
      481 ctctacatta agcataaagg ttatcggaaa ggcgcaatat tcacagttgt ttgttggttt
      541 gtcatttccc tcgcaatatt cagtattaga catttatagt ttggaaaatg cgtgataatt
      601 agttgtattc agttattaag taataaatta ttggaggcag aacatcatga aattaacatt
      661 aatgaaattt tttgtggggg gatttgcagt attattaagt tatattgtat ctgtaacact
      721 accttggaaa gaatttggcg gtatatttgc aacgtttccg gcagtatttt tagtgtctat
      781 gtttattaca ggtatgcaat atggtgataa agtcgctgtg catgtaagtc gtggcgcagt
      841 gtttggtatg acaggggtat tagtttgtat tttagttaca tggatgatgt tacatatgac
      901 gcacatgtgg ttgattagca ttgttgttgg tttcctaagc tggttcatca gtgcagtatg
      961 tatttttgaa gcggtagaat ttatagcaca aaaaagatta gaaaagcata gttggaaagc
     1021 tggaaaatcg aatagtaaat agtgtgaacg taatctctta actaggacta actttgcaag
     1081 cattgaatag catggaaaag ttgcatcatt aataagtgaa attcaagttg gcattgagaa
     1141 aattacaagc gcgtaatcat acaggtctgt cttaagggag tcttcgaacc ccgatgttgt
     1201 cgtatgtcaa aacatttagt caatcataaa ggtgacttga tttaacttta tctgatagtc
     1261 tgattgtaat gattgtacta attgactgga ggcgtatgta attgaatctg agtaaacaaa
     1321 ttaaaaagta tagggaacga gatggttatt cacaagaata tcttgctgaa aagttatatg
     1381 tatctaggca gagtatttct aattgggaaa atgacaaaag cttaccagac atacataact
     1441 tattaatgat gtgtgaattg ttcaatgtaa ctttagatga tttagtaaaa gggaccattc
     1501 catttgtacc tgatattaaa gcgcaacgaa gtcttaactt atggacatat gtgatgctta
     1561 ttttcatgac attagctgca attttaatgg gacctttagt tgtttattgg aattggactt
     1621 ggggtgtaac ggtggcaatc attttgggaa taggttttta tgcatctatg aaaatagaag
     1681 atttaaaaaa agtgcataaa atggacaact acgatcgaat tgttgctttt atgaatggaa
     1741 aagatcctag tgaagtacaa acgactaaag ctagaaatac gatgacaaat gcgctttcta
     1801 ttatatcagt aattggtata ctcagcctca taattttcct tagtgtgtat ttggcaaata
     1861 agtttttata aatcatcgtg gtatcgtctc atattattta tattatccaa aatagcataa
     1921 aaaaatacca acaagattta gaaccttgtt ggtaatcaaa gcgattcatt tataatgagt
     1981 cgttttatgt tgtaagatta aacagtttgt acgttaactg cttggtctcc acgttgacct
     2041 tcagtgattt cgaaagtaac tttttgacct tcttctaaag ttttgtagcc atcgctagcg
     2101 atacctgaga aatgtacgaa tacgtctccg ccattttctt gttcgatgaa accaaaacct
     2161 ttttctgcgt taaaccattt tactgtaccg ttattcatat agaaaacctc cgtgtgcttt
     2221 tgcacttaat atttgtaaca aattcataac taaaaaagag gatattctaa acaaatacac
     2281 tacaatttaa ttcacgagct tttattacgt aagaccaact atacgctcat attggcataa
     2341 tgtacagtgt tttttgaaaa taaattaaaa aagattttta aaaaccttag aaacgttgat
     2401 ttaaaggggt ttataaaaat aaaaaaattg tagtctttta tggtgtttgc tagttttcaa
     2461 agtgacatat cgtttaaaca tgatgatttt ataagcaatc cataaaaaac aagcagcgat
     2521 aaacgctact tgttgatatt aaaatctgac ttgaaaggtc atagcaatgt tctataccga
     2581 tggaatgtgc ttacttgcct ttttcttcac gacgttttaa ataataag
//
LOCUS       DANRDI010000246         2586 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.251, whole genome shotgun sequence.
ACCESSION   DANRDI010000246 DANRDI010000000
VERSION     DANRDI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.251"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            65..505
                     /locus_tag="PZ377_002736"
     CDS             65..505
                     /locus_tag="PZ377_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDM6359581.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            498..1709
                     /locus_tag="PZ377_002737"
     CDS             498..1709
                     /locus_tag="PZ377_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6359582.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1811..2161)
                     /gene="sarV"
                     /locus_tag="PZ377_002738"
     CDS             complement(1811..2161)
                     /gene="sarV"
                     /locus_tag="PZ377_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDM6359583.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            2287..2457
                     /locus_tag="PZ377_002739"
     CDS             2287..2457
                     /locus_tag="PZ377_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359584.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
BASE COUNT          798 a          447 c          389 g          952 t
ORIGIN      
        1 ttataattac tattgttgta aaaaggttag ctaagctaac tattttgtct taggagatgt
       61 cactatgcta tcacaagaat ttttcaatag ttttataaca atatatcgcc cctatttaaa
      121 attagccgag ccgattttag aaaaacacaa tatatattat ggccaatggt taatcttacg
      181 cgatatcgct aaacatcagc ccactactct cattgaaatt tcacatagac gtgcaattga
      241 aaagcctact gcaagaaaaa ctttaaaagc tctaatagaa aatgacctta taacagtaga
      301 aaacagttta gaggataaac gacaaaagtt tttaacttta acacctaaag ggcatgaatt
      361 atatgagatt gtttgtcttg atgtacaaaa gctccaacaa gcagtagttg ccaaaacaaa
      421 catttcgcaa gatcaaatgc aagaaaccat aaatgtgatg aatcaaattc atgaaatatt
      481 attaagggag gcacacaatg actaatcaag gctcattaaa taaatcacct attttcacca
      541 aaagttttac aattaacttt ttagtcaact ttatcgtata cctatgcatg tatttgttac
      601 ttgtagttat tgctggctat agtaagcaag ctttcgacgc ttccgattca ttagccggat
      661 tagttgtagg actatttatt gtcgggtctt taattggtcg atttgctaca ggtaaatttg
      721 taaatcaaat cggacctaaa cgattattat ttattggttt aattgcttta attatcacgc
      781 aattacttta ttttattgat ggatctttag cttttttaat ctttgtacgt ctcataaacg
      841 gtatcgcaac tgcagttgtg acaactgcaa caggtactat tgcggcatac gttacccctg
      901 ttaatagaaa aagtgaaggc atcagtttat tttccttaag tttagtatta ggtacagcga
      961 ttggcccatt tctaggcatg ttacttatta caaaatacgc aattgactta ctgtttatta
     1021 tttgtgtcat attaggcata ctcggactca ttatctcatt atttataaaa gttgatttcg
     1081 aagtaacaaa taacaaaact gaaactaatg tcattgataa accaagattt agtattcatc
     1141 aatttattgc aaaagaggcc attcctgttg ctgtaattat gcttttaatt ggcgttacct
     1201 atgcgtccat cctaacttat ttacaagcat ttgctttaga gcgacaccta gtcactgcag
     1261 caagctattt ctttatttgc tatgctatag catcgttaat tactagacct attgctggta
     1321 gattgatgga tgataaaaat gaaaatatta ttgtctaccc agcatttatc atgcttttcc
     1381 tttcatttgt atgcttaatt tcaagctatc aaagctggtt aatattactt gctggtgctt
     1441 gcctaggttt agggtatgga aatttatcat ctgcaatgca atctattgcg ataaaagtct
     1501 cacccccgat taaatatggt atcgcgacat ctacttttta cgttggtcta gatgcaggtg
     1561 tcggcttcgg tccatcattc cttgggttat ttactcatat gttttcatat agtgaaatct
     1621 tcggctttat ggccgcttta gcaatcatta ctatgcttgt ttacttctta attcatggac
     1681 gtcatgtaac tagaaatgcc atgaactaaa tatttatatt caaaaataaa aagcatgcca
     1741 atctctattc atcatttgtc tgtccccaag acgcaattat gatgtactga ttggcatgct
     1801 ttttaagttt ttagtttgta acgcttaaca cttctatttc tttagtaatt ttatctaaca
     1861 atttcttttg agaagtacta tagaaataga atacttgtct ttcatccgtt tcagaacgca
     1921 atttagaaat aagctctttt ttatagattt tacggataat cacatcgatt ttacttgtat
     1981 cccatagcat ttcaaaatgt aatgtatccc tcaattcttt accgctaatc ttttcagctt
     2041 catacacttt aaaaaggact acaaattctt caattgaaat cttatgtgtt gtttttaacc
     2101 agtgctttaa ctgacttaac tccctttctg cttctataaa acgttgaact ttattactca
     2161 taatatgatg cctcctatgt ttcttcttag atgtttgtaa ttcatattat tatacaatta
     2221 cttaaattta ttatatatca aacttagcat tttatctatt acaaatatta aattgctggc
     2281 tatatcttgt ttagatttat atcttttgga caacttgttg tttattcgac aacatttata
     2341 cctaaaaaag atatattaca actcaatggc atggagtgtg acatcaatgt taaagaacca
     2401 ctaaagattt attatgtagt gattcttaag agtgggatag aaatgatatt ctcataaaat
     2461 ttatttcgtt gatccccaac ttgcattgtc tgtagaattt cttttgaaat tctctatgtt
     2521 ggggccccgc caacttgcac attattgtat gctgactttt cgtcaccttc tgtgttgggg
     2581 ccccgt
//
LOCUS       DANRDI010000247         2505 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.252, whole genome shotgun sequence.
ACCESSION   DANRDI010000247 DANRDI010000000
VERSION     DANRDI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.252"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1676)
                     /locus_tag="PZ377_002740"
     CDS             complement(<1..1676)
                     /locus_tag="PZ377_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDM6359585.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKH"
     gene            complement(2358..>2505)
                     /locus_tag="PZ377_002741"
     CDS             complement(2358..>2505)
                     /locus_tag="PZ377_002741"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDM6359586.1"
                     /translation="PTKKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKK
                     NHKA"
BASE COUNT          703 a          468 c          328 g         1006 t
ORIGIN      
        1 tgatgttttg aattttcatg tgtcgattct tcaaagtcta catgaataga atcttcttca
       61 gtttcaatgg taccttctgc atgaccttct gcaccttcaa cagctgtatg atattcaaaa
      121 tcaattggct tagaatcatt actttcttcg attgtaccag tcaattcatg cttctccact
      181 ggcggctctg atttaaattc aagttcgata ggagtactat gttctataat aggttccttt
      241 agtttatctt tgccgtcgcc ttgagcgtta ttagagtaaa atgcaacgcc atttttccaa
      301 gttaaattac ttgtataata atagttataa tatccaaaaa ggtgtgtttg aaattctaag
      361 ttgctagcat ttgaatcata atacccttca tattttatta cataattttt actttggtct
      421 aaattattaa agtttaaaga ataaccacca ttagtatcaa aatctaaact catattatca
      481 gtcacatctt caaatttgct gacatcatca agctttgcat atacgctttc agctaaatcg
      541 tctgaaccaa tgtgtttata taccttaact gttggattat taacccctgg tttatttcct
      601 ttagttactt gaccagttac tgtcacagag cttaacgact ggttgttagg tttcatgtac
      661 gcaaaatgac taaatttccc atctacttta tttaaagtat caattcgacc attagctgtt
      721 actccccaat tatctctaac tccacctaaa tattgaatat taaatatttt gctaaccgta
      781 gtctcaccca atttaacttc aacattttgg ttaccttttt gcgtcactgt tgtaggatca
      841 ataaatagat ttaaagataa ttcagcagtt aaatctttct tttcttgtac atattcttta
      901 aacgtatatc taacttttct ttctccaatt atttctcctg tcgccataac ttgaccatct
      961 gtacttttta tctccggaac tttacgcagt gttgagatac catgagtttc aacattatcg
     1021 cttaatgtga aatcaaaata atctcccgcc ttaattcctt ctccaaattt ccatttatat
     1081 ttcaaggtta ctctttctgc gttatgagga tttacaacat tcgtatcttg tttatgtcct
     1141 acaatttcac taccttcttc tacttccact ttatttgtta catctgtacc tgtcgcttta
     1201 gtttcttcca ctacttcttt ctctgcaact gctgtaacgt cagttgatct tttcattctt
     1261 ggtttaattt ctgagacgtt acttggttga gctatgtcaa cttgagttcc tgtagtttcc
     1321 ttatcagcaa ctttttccga tggcaaatca actcgcgaag tttctacttt tggtgcttgc
     1381 acagttttcg gtgcttcttc tgttgttact tgtgttgatt gtgatggttg ctcagttgat
     1441 gtcgcgctgt atgattgtgt ttcatctatt gtattaacgt tatttgtagt tgtttgtgtt
     1501 tcgcttgctt tactttcagt agctgaactc ccactttcct ctactgtagt attgttttgt
     1561 tccgatgctg cagcttcttt ttcttgtccc attccaacaa cgatcattgt tcctaagaat
     1621 actgaggccg ctcccaattt gtgttttctt atgccgtatc taagattgct tttcactata
     1681 atattctccc ttaaatgcaa aattcattta tttttaaaac tcaataaatg caattctata
     1741 ttgttcggtt tttaaaagca atgaaaaaaa gcgagttaat aaaaagttaa gattgttgtt
     1801 aactttatgt ataatgagtt ttttattatt tgaaactcac atatatattg catacaaagc
     1861 tcttgaacac cttgatataa caggcttgta ttttttatac ttacttttta aattaaattc
     1921 aaattcatct aatttaaaac aatatactaa accatacata ataatcgcct gtacaatgca
     1981 tcattaacaa gtcactgaaa cgcctttcat tgtattaata acgtcactat aatttttata
     2041 tcgttcggtt tttgtttgat tttaatgatt atttatacaa aaacagccgt atttcaagcc
     2101 gacattttaa atttaactaa atttgcatct agttaataat tgcatttatc aaatttgtct
     2161 tattgatcca atctaatttg tactcacaaa ctagtttaaa attctaactt tatctctcag
     2221 ttcgttatca atcatcagac ataaaccaat gaagcaatca gaaaacactc taattttcta
     2281 ttagaaattt gatttaatat aaaaaaacag gcttacttca tataatttat gaaataaacc
     2341 cgtcaatttt tgtttaatta tgctttgtga ttctttttat ttctgcgtaa taatgctaaa
     2401 cctagaatgc tgaataatcc gccgaacaac atacctttgt ttgttgattc ttctccacct
     2461 gtttcaggta gttcagattt cttagattgt ggttttttag ttggt
//
LOCUS       DANRDI010000248         2500 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.253, whole genome shotgun sequence.
ACCESSION   DANRDI010000248 DANRDI010000000
VERSION     DANRDI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.253"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..787
                     /locus_tag="PZ377_002742"
     CDS             <1..787
                     /locus_tag="PZ377_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDM6359587.1"
                     /translation="LLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLFLEIDIDN
                     HRYICLSSKFNFEKREKEIPFEDRDSCYFLAMGYFYNKEDSNEIIKGYENNYDRGINI
                     PRAHSIYLYEYYWSEAYKNYKEGYLTESDGKLCPAIYEYFWELDYSVKDKSISFYIPC
                     KEIVDYFSLIQTEEGVWKTKFGETICINSKLLEFDNECLLIKKESLLNFLNTKKLSIG
                     WKIYLEKISLRDRQEWWYNVFYDDGKYNKKIIKNDMSKIRRNF"
     gene            1308..1685
                     /locus_tag="PZ377_002743"
     CDS             1308..1685
                     /locus_tag="PZ377_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDM6359588.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSISGYINNDKKLSFTAGIRSVDDFQFDTDISYTDEL
                     GRKFNKNPKSVSEIKKEQNTSNK"
     gene            1879..2055
                     /locus_tag="PZ377_002744"
                     /pseudo
     CDS             1879..2055
                     /locus_tag="PZ377_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2033..2134)
                     /locus_tag="PZ377_002745"
     CDS             complement(2033..2134)
                     /locus_tag="PZ377_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359589.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(2257..2352)
                     /gene="pepA1"
                     /locus_tag="PZ377_002746"
     CDS             complement(2257..2352)
                     /gene="pepA1"
                     /locus_tag="PZ377_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HDM6359590.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
BASE COUNT          993 a          295 c          378 g          834 t
ORIGIN      
        1 attacttgaa attgataaag aatcaaaaat actagtaccc tcaccacttc cttcgcatca
       61 atcgaatgaa tgggtaaaaa ataccaaagt ttttgatgaa acaaaattat ttttagaaat
      121 agatatagac aatcatagat atatatgttt aagctcaaag tttaattttg aaaagagaga
      181 gaaagaaata ccatttgaag atagagactc atgttatttc ttagcgatgg gatattttta
      241 taataaagaa gatagtaatg aaattatcaa aggttatgaa aataattatg atagaggtat
      301 taatattcct cgagcacata gtatatattt atatgaatat tattggagcg aagcttataa
      361 aaattataaa gagggatatt taactgaatc agatggtaaa ttatgtccag ctatttatga
      421 atatttttgg gagttagatt attctgttaa ggacaaatca ataagttttt atattccgtg
      481 taaagaaata gtagattatt tttcattaat acaaacggaa gaaggagtat ggaaaactaa
      541 atttggtgaa acaatatgta ttaattctaa attactcgaa tttgataatg aatgtttact
      601 tattaaaaaa gaaagtttat taaactttct taatactaaa aaattgtcta ttggatggaa
      661 aatatatcta gaaaaaattt ctcttcgaga tagacaagaa tggtggtaca atgtttttta
      721 tgacgatggc aaatataata agaaaataat taagaatgac atgtcaaaaa ttaggcgtaa
      781 tttttagatt tatctatata tttatgccac cgctcaaact tttataaaaa gtaaaaaaac
      841 aatatttata ttgaacttgt gacgtgaatt tctaaaacac aaccaacaat ccacaaggaa
      901 actagtaaat aagtttaaat aagatttcga aaaaatgtgt aatattataa tatgaaaatc
      961 tttaagatta tagaagaatc atactgacta aaaagatttt gaaagtgatt aagtatttaa
     1021 aacacgccaa tcttaaaccg ctattgacaa atatgaagcg gaaccaccgt tgtttttctt
     1081 ttcaggtcca attgaataaa tgttatgaat agaatggata aaaatataat tatttaagtg
     1141 ttaaagtaaa ccaaatcatg atttaagatt ataagaaaga aaaattgact aatgttaaaa
     1201 ccaacaatca tatgggttgt aacgtttata caatcatcgt aaaactcaat atgcttaatt
     1261 tattcaaaat gttcgtcaat aagtacaatg aaaaggatat tgattttttg aataaaaaat
     1321 atcttatgat tgtaattata attttaatat tgattctagg cggaatcttt ttgaagatga
     1381 aatatgacga aaagaaatat tatgatgaac aaaaagaaag aataacgatt tatatgaagt
     1441 acaatgtgaa aggttataaa aatataagct tcgctaattt taaagaaaac ccaatggatg
     1501 gttattctat tagtggttat ataaataatg ataaaaagtt atcatttaca gctggtataa
     1561 gatctgttga tgattttcaa tttgataccg atatttctta tacagatgaa ttgggtagaa
     1621 aatttaataa aaatcctaag tcagtttctg aaataaaaaa agagcaaaat acgtccaata
     1681 aataattgtt catattgtga tgaaacaaaa atataagtca ttagatgagt ttaactatgt
     1741 tataaatatt tgtagtatct ataaaaatct cgacactatt aaaatgataa agtgccgagg
     1801 ttttcttact tatttagtta attcaaagtt tatgccagat tcataagaat ttgtgacact
     1861 tttaatagtg taaccattga ttattacaat ttatcaaatg gtcctttaga aggtataaat
     1921 aacaaaataa aattaattaa acgtacatct tttggttatg gaagttacaa tcatttgcgc
     1981 aatcgaatat tattatgttc aaaactttac gctccaaaaa gtaaaaagga agttaagcaa
     2041 tgtttagttg cttaacttcg gatattgaac gcatcagtcc aatttgacat agagcctttt
     2101 ttagttcttg atgtttctct ttaaaacctt gcatatttta caaaaagaaa aattagcagt
     2161 ataattaaga caacgaaaat aagtatttac ttatacacca atcccctcac tatttgcggt
     2221 agtgagggga tttttattgg tgcggctata tgtcacctat tttgtattgc gtctacttag
     2281 ccaataagaa aaaaacgcaa tggcacagcc actgatgact ggtgctatga tgtgaacgaa
     2341 aataagcatc accttataca cctcctctct gcgtctaaat tgacgcctga gagataggcg
     2401 actctactat tatatcatcg gcaaatatac aagcacagtc acttgcttct gataagttat
     2461 atgattctag ctgatagatt gaatcgtcta cacttaattg
//
LOCUS       DANRDI010000249         2418 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.254, whole genome shotgun sequence.
ACCESSION   DANRDI010000249 DANRDI010000000
VERSION     DANRDI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.254"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>2418
                     /locus_tag="PZ377_002747"
     CDS             <1..>2418
                     /locus_tag="PZ377_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HDM6359591.1"
                     /translation="SNNENNADIILPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLIT
                     SNTTLTVVDADKNNKIVPAQDYLSLKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPED
                     IKNIGDIKDPNNGETIATAKHDTANNLITYTFTDYVDRFNSVQMGINYSIYMDADTIP
                     VSKNDVEFNVTIGNTTTKTTANIQYPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQ
                     TIYVNPSENSLTNAKLKVQAYHSSYPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNT
                     KELTDVTNQYLQKITYGDNNSAVIDFGNADSAYVVMVNTKFQYTNSESPTLVQMATLS
                     STGNKSVSTGNALGFTNNQSGGAGQEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTV
                     FDNNTNTKVGEAVTKEDGSYLIPNLPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNG
                     LSSVITVNGKDNLSADLGIYKPKYNLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDEN
                     GNVLKTVTTDADGKYKFTDLDNGNYKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGV
                     INGADNMTLDSGFYKTPKYNLGNYVWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQ
                     TTKTDKDGKYQFTGLENGTYKVEFETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKD
                     NDTIDSGFYKPTYNLGDYVWEDTNKNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDE
                     NGKYQFTDLNNGTYKVEFETPSGYTPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDS
                     GFYKTPK"
BASE COUNT          998 a          349 c          410 g          661 t
ORIGIN      
        1 ttcaaataat gaaaataatg cagatatcat tttgccaaaa agtacagcac ctaaacgttt
       61 gaatacaaga atgcgtatag cagcagtaca gccatcatca acagaggcta aaaatgttaa
      121 tgatttaatc acatcaaata caacattaac tgtcgttgat gcagataaaa acaataaaat
      181 cgtaccagcc caagattatt tatcattaaa atcacaaatt acagttgatg acaaagttaa
      241 atcaggtgat tatttcacaa ttaaatactc agatacagta caagtatatg gattgaatcc
      301 ggaagatatt aaaaatattg gtgatattaa agatccaaat aatggtgaaa caattgcgac
      361 tgcaaaacat gatactgcaa ataatttaat tacatataca tttacagatt atgttgatcg
      421 atttaattct gtacaaatgg gaattaatta ttcaatttat atggatgctg atacaattcc
      481 tgttagtaaa aacgatgttg agtttaatgt tacgataggt aatactacaa caaaaacaac
      541 tgctaacatt caatatccag attatgttgt aaatgagaaa aattcaattg gatcagcgtt
      601 cactgaaaca gtttcacatg ttggaaataa agaaaatcca gggtactata aacaaacgat
      661 ttatgtaaat ccatcggaaa attctttaac aaatgccaaa ctaaaagttc aagcttacca
      721 ctcaagttat cctaataata tcgggcaaat aaataaagat gtaacagata taaaaatata
      781 tcaagttcct aaaggttata cattaaataa aggatacgat gtgaatacta aagagcttac
      841 agatgtaaca aatcaatact tgcagaaaat tacatatggc gacaacaata gcgctgttat
      901 tgattttgga aatgcagatt ctgcttatgt tgtaatggtt aatacaaaat tccaatatac
      961 aaatagcgaa agcccaacac ttgttcaaat ggctacttta tcttcaacag gtaataaatc
     1021 cgtttctact ggcaatgctt taggatttac taataaccaa agtggcggag ctggtcaaga
     1081 agtatataaa attggtaact acgtatggga agatactaat aaaaacggtg ttcaagaatt
     1141 aggagaaaaa ggcgttggca atgtaactgt aactgtattt gataataata caaatacaaa
     1201 agtaggagaa gcagttacta aagaagatgg gtcatacttg attccaaact tacctaatgg
     1261 agattaccgt gtagaatttt caaacttacc aaaaggttat gaagtaaccc cttcaaaaca
     1321 aggtaataac gaagaattag attcaaacgg cttatcttca gttattacag ttaatggcaa
     1381 agataactta tctgcagact taggtattta caaacctaaa tacaacttag gtgactatgt
     1441 ctgggaagat acaaataaaa atggtatcca agaccaagat gaaaaaggta tatctggcgt
     1501 aacggtaaca ttaaaagatg aaaacggtaa cgtgttaaaa acagttacaa cagacgctga
     1561 tggcaaatat aaatttactg atttagataa tggtaattat aaagttgaat ttactacacc
     1621 agaaggctat acaccgacta cagtaacatc tggtagcgac attgaaaaag actctaatgg
     1681 tttaacaaca acaggtgtta ttaatggtgc tgataacatg acattagata gtggattcta
     1741 caaaacacca aaatataatt taggtaatta tgtatgggaa gatacaaata aagatggtaa
     1801 gcaggattca actgaaaaag gtatttcagg cgtaacagtt acattgaaaa atgaaaacgg
     1861 tgaagtttta caaacaacta aaacagataa agatggtaaa tatcaattta ctggattaga
     1921 aaatggaact tataaagttg aattcgaaac accatcaggt tacacaccaa cacaagtagg
     1981 ttcaggaact gatgaaggta tagattcaaa tggtacatca acaacaggtg tcattaaaga
     2041 taaagataac gatactattg actctggttt ctacaaaccg acttacaact taggtgacta
     2101 tgtatgggaa gatacaaata aaaacggtgt tcaagataaa gatgaaaagg gcatttcagg
     2161 tgtaacagtt acgttaaaag atgaaaacga caaagtttta aaaacagtta caacagatga
     2221 aaatggtaaa tatcaattca ctgatttaaa caatggaact tataaagttg aattcgagac
     2281 accatcaggt tatacaccaa cttcagtaac ttctggaaat gatactgaaa aagattctaa
     2341 tggtttaaca acaacaggtg tcattaaaga tgcagataac atgacattag acagtggttt
     2401 ctataaaaca ccaaaata
//
LOCUS       DANRDI010000250         2382 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.255, whole genome shotgun sequence.
ACCESSION   DANRDI010000250 DANRDI010000000
VERSION     DANRDI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.255"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            280..1128
                     /locus_tag="PZ377_002748"
     CDS             280..1128
                     /locus_tag="PZ377_002748"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDM6359592.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            1142..1966
                     /gene="purR"
                     /locus_tag="PZ377_002749"
     CDS             1142..1966
                     /gene="purR"
                     /locus_tag="PZ377_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDM6359593.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            1983..2363
                     /locus_tag="PZ377_002750"
     CDS             1983..2363
                     /locus_tag="PZ377_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDM6359594.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
BASE COUNT          876 a          295 c          467 g          744 t
ORIGIN      
        1 tagtataaga agtaaatatt atgataatta tttgagtgtt gggcattatg ttcaatactc
       61 tttttattta caaaatgttt aacactgatg tttcgcttat agatttttca gtaaatggat
      121 aattgtattt ataaacacaa atacaagtaa atactaagta attagatgga gaaaattact
      181 tttttattaa aaaaacacta aaaaacaaat taaaatgtca aatattaatt ctctttatgt
      241 taaaatcatc atattaagat aacgaaaaga gggcggaaaa tgatatatga aacggcacca
      301 gccaaaatta attttacgct cgatacactt tttaaaagaa atgatggcta tcatgagatt
      361 gaaatgataa tgacaacagt tgatttaaat gatcgtttaa cttttcataa aagaaaagat
      421 cgaaagatag ttgttgagat tgaacataat tatgtgcctt ctaatcataa aaatctcgca
      481 tatcgtgcag cgcaactatt tattgagcaa tatcaactaa agcaaggtgt aacaatttct
      541 atcgataaag aaatacctgt ttctgctggc ttagctggag gttcggctga tgcagcagca
      601 acgttaagag gattgaatcg actttttgat ataggggcga gtttggaaga attggctcta
      661 ctaggcagta aaatcgggac agatattccg ttttgtattt ataataaaac tgcactatgt
      721 actggaagag gagagaaaat cgagttttta aataaaccac cttcagcttg ggtgattctt
      781 gctaaaccaa acttaggcat atcatcacca gatatattta agttgattaa tttagataag
      841 cgttacgacg tacatacgaa aatgtgttat gaggccttag aaaatcgaga ttatcaacaa
      901 ttatgtcaaa gtttgtctaa tcgattagag ccaatttctg tttcaaaaca cccacaaatc
      961 gataaattaa aaaataatat gttgaaaagt ggtgcagatg gtgcgttaat gagtggaagc
     1021 ggacctactg tgtatgggct agcacgaaaa gaaagccaag caaaaaatat ttataatgca
     1081 gttaacggtt gttgtaatga agtgtactta gttagactat taggatagaa gggttgaaaa
     1141 gatgagatat aaacgaagcg agagaattgt ttttatgacg caatatttga tgaaccatcc
     1201 gaataaattg attccattaa ctttttttgt gaaaaaattt aaacaggcga agtcttcaat
     1261 aagtgaagat gtccaaatta taaaaaatac attccaaaaa gaaaagttag gtacagtaat
     1321 tactactgct ggcgcaagtg gtggtgttac gtataaacca atgatgagta aagaagaggc
     1381 gactgaagtt gttaatgagg tcattactct attagaagag aaagaacgtt tgttacctgg
     1441 cggatattta tttttatcag atttggtagg taatccatcg ctactaaaca aagttggtaa
     1501 gttaattgcc agtatttaca tggaagaaaa attagatgct gttgttacca ttgcgacaaa
     1561 aggtatttca ttggcaaatg cggttgctaa tattttaaat ttaccagtag tagtgattag
     1621 aaaagacaac aaggtgactg aaggttctac agtttcaatt aattacgttt caggatcttc
     1681 aagaaaaata gaaacaatgg tactttcgaa gagaacttta gcagaaaatt caaatgtttt
     1741 agttgtcgat gattttatga gggctggtgg ctctattaat ggtgttatga atttaatgaa
     1801 tgagtttaaa gcccatgtaa aaggggtatc agtacttgta gaatcaaaag aagttaaaca
     1861 aagattgatt gaagattata cttccttagt gaaattatct gatgtagatg aatataatca
     1921 agagtttaac gtagaacctg gcaacagttt atctaagttt tcataaaagg agttttagta
     1981 ttatgaaaat cattaacaca acaagattac cggaagcact tggaccatat tcgcatgcaa
     2041 cagttgtgaa tggtatggtt tatacttctg gtcagattcc attgaatatt gatggacata
     2101 tcgtaagcgc tgatgttcaa gcacagacaa aacaagtttt agaaaattta aaggttgttt
     2161 tggaagaagc aggatctgat ttgaattctg ttgcgaaagc gaccattttc attaaagata
     2221 tgaatgattt ccaaaaaata aatgaagtgt atggtcaata ttttaatgaa cacaagccag
     2281 cgcgtagttg tgtagaggtt gcgcgtttgc caaaagatgt gaaagtagaa attgaattag
     2341 taagtaaaat taaggaatta taattttcga ttaatatgtt ta
//
LOCUS       DANRDI010000251         2378 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.256, whole genome shotgun sequence.
ACCESSION   DANRDI010000251 DANRDI010000000
VERSION     DANRDI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.256"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(213..1874)
                     /locus_tag="PZ377_002751"
     CDS             complement(213..1874)
                     /locus_tag="PZ377_002751"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDM6359595.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(1871..2287)
                     /locus_tag="PZ377_002752"
     CDS             complement(1871..2287)
                     /locus_tag="PZ377_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDM6359596.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
BASE COUNT          717 a          476 c          309 g          876 t
ORIGIN      
        1 gttcagtcaa ctactgccat tataactttg tagagcatag aacattgatt tatgtctcag
       61 gctctcttat gcagttgtgc agagcatcca actgtgttcc tcttaacctt attcacatac
      121 taagcattcc ccaatatgtc acatacctaa aaaaacactc caacacaaag tatctttcga
      181 gaaactctac gttggagtgt atataaatgt tcttacattg attcaggtgc gcttacaccg
      241 accattgcca atgcattttt caatgtaatt ctgaccgctt caatcatagc aacatgtgct
      301 tttgtttttt caatatcatc tgttaacact ttttcagcat tatagaattt atggaaatga
      361 gaagctaaat cttgaatata attagtaatt ctatgtgccg atctatgctc agcagcactt
      421 tcaattgtag gttcgaaatc agctactttt ttcaacaatt caatcgcttt ttcattagta
      481 atcgttgtaa aatcattcgc agcagtcact tcaatacctt gctctttcgc ttgttttaaa
      541 attgaacaaa tacgcgcatg tgcatattga gcatagtaaa ctggattgtc ttgagattgc
      601 tctttcgcta attccatatc aaaatcaaag tgactatcag gactacgcat agttaagaaa
      661 taacgtgcag cgtcaacgcc aacttcgtcc ataatttctc ttaatgtaat cgcattacca
      721 gtacgtttac tcatcttcac ttctttacca ttttccatta aacgaaccat ttgcatgatt
      781 tgaatttcta aacgattact atctacacca aacgtttcaa gagatgcttt caaacgatta
      841 atataaccat gatgatcagc accaaataaa tcgattaaaa tgtcattacc acgttttact
      901 ttatcgaagt ggtacgcaat atctggtaag aaatacgtat atgtaccgtc atttttaatt
      961 aatactctgt ctttgtcgtc tttaaaatca gttgtacgta accatgtagc gccatcagct
     1021 tcatacgtat aacctaattc tttcattttt gctaaaactt caagaatttc tcctttttca
     1081 tataaagatg tttcactaaa ccaattatca aaatgcgtat tgaactctgc taaatcattt
     1141 ttcaatttag ccatttcgta ttctacgcct aattttctaa attctttcaa acgtgcttct
     1201 tcagaataat ctttaatttc agggtgtttc tctgctaaat ctttacctat ttcaataata
     1261 tcttttccat tatagccatc ttctggcatt gaataactat tgtcacctaa agcttcaaag
     1321 aaacgtgttt caatcgaacg cgctaagtta gtaatttgat taccagcatc attaatataa
     1381 tattcacgtg ttacattata gccagctgca gttaaaatat tagctaaagc atcaccaact
     1441 gctgcatttc tagcatgacc aatatgtaaa tctcctgtag ggttagctga aacatactca
     1501 agcaatacat tttgaccttt tgattcattt acatgtccaa attgatcacc tttttcaatt
     1561 gcttcaggaa taattgctgt taaatactga ttatctaagt aaaaattaat gaatcctgga
     1621 ccagcaatgt caatttgttt tacatgtgct ttttcagtat ctaagttatc aacaatcgct
     1681 tgagcaattt cacgaggatt acgctttgca atcttagtca gtaccatcgc aatattagta
     1741 gcataatctc cattttttgt atctttagga acttcaattt taatatcagg aatctcatct
     1801 gctaatcctg ctttgttaat acttgctgca atttcttcta ctaatgtttg tttcacttga
     1861 tcaataatat tcattcgcct atctccttat agttaatttc atattgataa gaacccattt
     1921 tttcattatc ttgatataat tcataatgaa tctttagctt gccaccgtct ccacttacga
     1981 aatgtaaaat gcgtaatgtt ttaacttcta gtggaattcg tccagccgat atatcgtaaa
     2041 aagttgttgt cgtttgtcct tcaacaaaat gcaaattcat attaatgtcg cctttacgaa
     2101 tcaacttaac gctatcatca tccactttta ttgtaacatt aactgttgca tcctcaattt
     2161 gttctacata ccgaataaag ttagattgcc tttgttgcca agttccttca gtagtaaatt
     2221 caaatttttc tttttcgttg tgctgtttca acacttgctt tgtttgaata cttacttttt
     2281 tatccaattt actactcacc ttaatcttcg cataatagaa atattatagc ataaaataag
     2341 gtcatcttgt tatgccttct taaagctaaa taggttca
//
LOCUS       DANRDI010000252         2371 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.257, whole genome shotgun sequence.
ACCESSION   DANRDI010000252 DANRDI010000000
VERSION     DANRDI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.257"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(69..1262)
                     /locus_tag="PZ377_002753"
     CDS             complement(69..1262)
                     /locus_tag="PZ377_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDM6359597.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINAGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
     gene            complement(1646..>2371)
                     /locus_tag="PZ377_002754"
     CDS             complement(1646..>2371)
                     /locus_tag="PZ377_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HDM6359598.1"
                     /translation="VNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTG
                     KVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTIT
                     TPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSG
                     PVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQ
                     KGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT          765 a          432 c          317 g          857 t
ORIGIN      
        1 aagccaaact aattttatat ttcatttcaa ttcccacctt aatagcattt attaaaattc
       61 tataatgttt acatagactt cttaacaaac tttaacccag ctaagctaag tattgaaata
      121 attacacctc ctaatataaa tatattactt aagcttagta aaggtataat tatacttatc
      181 agtcctaaag acaatgtatc cgctgcataa ttcgatgtag atgagatact aaatactttc
      241 cccatcaaat gatttggcgt ttttatttga atggcaactg atcttgttag tccctctata
      301 gattgtccaa gtcccaataa tgttgcacct atatataata tcgccacact tggaaacaca
      361 ttaataatcg ataagccaat tccccaaact aaaacaccaa tactaaattt aaagattaat
      421 cgcttttctg acagcaaacc cataatcaat gacattaata aagatgctat acctaaacat
      481 gatgtagcta gtccatatac accaacgccc tcttttaata tattggaaat aaacaatggt
      541 aatacaacac gccaaaggcc agcattaatc aatatgcagg caaattggat gattataata
      601 aatggaattt ctttagattg tttcaagaat tcccaagttt cagaaaaatc ttcttttgag
      661 tgtctatcaa tcatgttgtt atttgtatat tttaaaagtg cattaaaaat aaatcctaaa
      721 aatagcaata tactacaaat aaaaaagacg ccaacattac caactagtat tacaatgaca
      781 ccaattaaag caggtaaaat aatatttgag cctctttgca aactatcgat taacgcatta
      841 cttgttgcta aatgctcctc atcaataatt tcaggaagaa ttgccctaaa cgcaggatcc
      901 gtatagcagt taataatggt gatagctgta gatatggtta gaagcgtcag ataatttaaa
      961 tttgatgtta ttgcaagtaa aggaataatt attataatca aacttagtat aagatcagat
     1021 agataaagta ttttcttttt attatgttta tcagaatatg cgccaccgaa aataccaaat
     1081 ataataaatg gaagtgtttg actcataacc atcattgata attttaaaga tgattggttt
     1141 gtcaattcaa cagtaaacca aattatttgt aacgaaaaca gcacaaaaca actccgacgt
     1201 aagaaattac caatcaataa atatgtaaag tttctatttt tcaaaacttc taaatacaac
     1261 atatttatca cctctcataa aaataattga atgcatccac cagctttttt agaccttctt
     1321 ctaaactctc tttatccaaa gcgcaattaa ttctaatata atttagtcag ttaaatatca
     1381 attatttcga aatatacata ctacttgaaa caccatacat aacccccaaa atgactactc
     1441 agaggttata ttctactaat tatgattata ttaaatatga aaatattatc aaaaaaatca
     1501 aatttataac aaaaatacac cccttaaagt taggtctttc aatccaactt ttggggtgta
     1561 tatcattctc atcatattct aggttgtttt taacaaacta aatatagtga atgcaaatca
     1621 actattattt aaattatgaa ttattttaat tctttcttct acgagccaat aacattaatc
     1681 cagcaattcc aattatacta ctaaagatca aacctttttg cgtgctttct aaacctgttt
     1741 ttggtaattc tgctcgtttt ttctcttgat tagctactga ttctttagca attttagatt
     1801 ttttaacttt atcattttta tccattgaat gaactgggcc atttggtttt gctctgtctt
     1861 tcgataatcc tggattgtta ggatttactg ggccacttgg atgagttggt ctgctcggct
     1921 tctctgggtt ttcaggtcct tttggatctt ttggtttctc tccaccgaac tctacaatct
     1981 tatctactgg ttgttttgtg atctcttctg ttggttgacc ctcgccaact ttttcacctg
     2041 ttaatgggtt cactgtgatt ggtgttgtga ttgtcttact tcctggttgt ccttcttgtt
     2101 tcactcgctc ttcaccaggt tgtaattttg gattaaactc acgttttgtt tcaaacggta
     2161 tctctactgt ttttgtttct ggtgtacccg tttttggtcc gtgtttaatc acatcatcca
     2221 ctggctcttc gatcactttt cctgtgtctg gattcttgat tcctggttta cctggtactt
     2281 tttccgtttg atctgtgggt aagtttggat caaagatatc tttatgacct tgcggtattt
     2341 tctcgccacc gaattctgtt aattcattaa c
//
LOCUS       DANRDI010000253         2349 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.258, whole genome shotgun sequence.
ACCESSION   DANRDI010000253 DANRDI010000000
VERSION     DANRDI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.258"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            123..1352
                     /locus_tag="PZ377_002755"
     CDS             123..1352
                     /locus_tag="PZ377_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDM6359599.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            1607..1699
                     /locus_tag="PZ377_002756"
     CDS             1607..1699
                     /locus_tag="PZ377_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359600.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            1877..>2349
                     /locus_tag="PZ377_002757"
     CDS             1877..>2349
                     /locus_tag="PZ377_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDM6359601.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVA"
BASE COUNT          741 a          315 c          453 g          840 t
ORIGIN      
        1 cacgagtcat aattcttcgt attttacata gcaatttaac tgattttaga gtccacggta
       61 cagaagtttg atatttcaat gtttctaaat ttttaaaaaa ttaaatcata ggtgggtgcc
      121 aaatgttttt attaatcaac attattggtc taattgtatt tcttggtatt gcggtattat
      181 tttcaagaga tcgcaaaaat atccaatggc aatcaattgg gatcttagtt gttttaaacc
      241 tgtttttagc atggttcttt atttattttg attggggtca aaaagcagta agaggagcag
      301 ccaatggtat cgcttgggta gttcagtcag cgcatgctgg tacaggtttt gcatttgcaa
      361 gtttgacaaa tgttaaaatg atggatatgg ctgttgcagc cttattccca atattattaa
      421 tagtgccatt atttgatatc ttaatgtact ttaatatttt accgaaaatt attggaggta
      481 ttggttggtt actagctaaa gtaacaagac aacctaaatt cgagtcattc tttgggatag
      541 aaatgatgtt cttaggaaat actgaagcat tagccgtatc aagtgagcaa ctaaaacgta
      601 tgaatgaaat gcgtgtatta acaatcgcaa tgatgtcaat gagctctgta tcgggagcta
      661 ttgtaggtgc gtatgtacaa atggtaccag gagaactggt actaacggca attccactaa
      721 atatcgttaa cgcgattatt gtgtcatgct tgttgaatcc agtaagtgtt gaagagaaag
      781 aagatattat ttacagtctt aaaaacaatg aagttgaacg tcaaccattc ttctcattcc
      841 ttggagattc tgtattagca gcaggtaaat tagtattaat catcatcgca tttgttatta
      901 gttttgtagc gttagctgat ctatttgatc gttttatcaa tttgattaca ggattgatag
      961 caggatggat aggcataaaa ggtagtttcg gtttaaacca aattttaggt gtgtttatgt
     1021 atccatttgc gctattactc ggtttacctt atgatgaagc gtggttggta gcacaacaaa
     1081 tggctaagaa aattgttaca aatgaatttg ttgttatggg tgaaatttct aaagatattg
     1141 catcttatac accacaccat cgtgcggtta ttacaacatt cttaatttca tttgcaaact
     1201 tctcaacgat tggtatgatt atcggtacat tgaaaggcat tgttgataaa aagacatcag
     1261 actttgtatc taaatatgta cctatgatgc tattatcagg tatcctagtt tcattattaa
     1321 cagcagcttt cgttggttta tttgcatggt aatatgtcga agagtgacta tgataataca
     1381 ttttaactaa taaatatgtc caggcatgtc gtctattgat ataggtgaga tgcttggact
     1441 tttttattat tgatataaag gtatttaaat atttttaaag ttaccgaaat tgaagcatta
     1501 taaaaaccaa gtgcacatgg taatacactt ggcttttatg ggaaatgaat attattgtac
     1561 atatgacagt aaggactagg tacagtcata gtacttcgag caaaatttgt tttgttatta
     1621 taaacaacac aaaggagata acttctctat tgaagaagtt aaaaacatta tagcagacaa
     1681 tgaaatgaaa gtaaattaaa aattcagaat atttttaatt attatattgt gagtgatatt
     1741 tattagggaa agctattctt catataaatt agttaaatag taaatctttg ttagaactct
     1801 ttccatagag ttgtgacagc atttttgttg tgctactatt tttatagtct aataaatata
     1861 taaaggggat ggtttcgtga ataaaacggt taaagattta atactagttg tcttaggttc
     1921 atttatcttt gctgcaggtg taaatgcatt tattatttct ggtaacttag gtgaaggcgg
     1981 ggttacaggt ttagcaatta ttttatatta tgcgtttcat atttcaccag ccatcactaa
     2041 cttcttggtc aacgcagtat tgattgccat aggttataaa tttttgagta agagaagtat
     2101 gtacttaact attcttgtaa caattcttat ttcaatattt ttgagtttaa cagaatcatg
     2161 gcaagtagaa actggaaaca gcattgtgaa tgccattttt ggtggtgtaa gcgttggact
     2221 aggaatcgga gtaattatcc ttgcaggcgg tacaacagca ggtacaacaa ttttggcgag
     2281 aattgcaacg aaatacctcg atgtaagcac gccatatgct ttgcttttct tcgatatgat
     2341 cgttgttgc
//
LOCUS       DANRDI010000254         2337 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.259, whole genome shotgun sequence.
ACCESSION   DANRDI010000254 DANRDI010000000
VERSION     DANRDI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.259"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..615)
                     /locus_tag="PZ377_002758"
     CDS             complement(<1..615)
                     /locus_tag="PZ377_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDM6359602.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVA"
     gene            complement(952..1560)
                     /locus_tag="PZ377_002759"
     CDS             complement(952..1560)
                     /locus_tag="PZ377_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDM6359603.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            1959..>2337
                     /locus_tag="PZ377_002760"
     CDS             1959..>2337
                     /locus_tag="PZ377_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDM6359604.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVL"
BASE COUNT          800 a          372 c          378 g          787 t
ORIGIN      
        1 tgctacttgg ctataatctt taaatgtatc gtcactatgt tgaggcttta agttataacg
       61 tttgattaaa aattttacaa ctggaccacc gataagccct ccaaatacaa gacctaaagt
      121 tgcagctgca agagccgctg tcagtgccga atcaatacct aaatcttgaa ttgtcttacc
      181 ataagcagca gcattaccat gaccgccttc catagacatg gaacctgctg ttaatcctaa
      241 caaaggttta atatttaata cttttgctag tgatacacca actatgtttt gaatgactga
      301 aatgatagca caaaacataa agtataatag caagactttg ccacctaatt taaataattt
      361 caatgatgca ccaagaccga ttgtcgtaaa gaatgctaac atgaagaaat cttgaatgaa
      421 tgaagcatct aatttaatct taaccatgcc aaatgaatcc aaagccgcaa ctaaaatagc
      481 aaagattaag ccgccaatca ctggtgctgg tatacaaata cgttttaaaa aattaacgtg
      541 attaacgata gccttaccaa gtaaataaag gatacaagct aaacataatg ttgtaattgc
      601 attaagttct atcattttca aatccccctt gttgaatatc cctctcccta aataagcttt
      661 tatatgtaag cgttataaaa gggcgctttt aacattatac atgcaacaaa tcaatatttc
      721 tagtttttga aaataatatc aataatatta atgcatctaa atttaataat tataaataat
      781 tctattgtta tattttataa ttaagaacaa gttcaataag aggagatgaa tcaaaaaata
      841 tgatggatac aaagaaaaac aatcaaccca tccgcatgtt gacggcacga ttgattgact
      901 tataaggaga tagatttcat tttcatgata gtaatcaatg aaagatggca tttatttcca
      961 agtatcttcg ggaaacatac aatctgattt acgcatgcga gacacaaatg gattaccttt
     1021 cactgtgtaa cgtaaagatt tatgtgtcca atcaccttta tttggaatac cgattcgtgg
     1081 actagcaata atatctttag gatatttacg attcttagta tcaatagaca atctacagtc
     1141 atttaacgta gcgccatcga tagcccgtgg aatgttaaat gccttagtcc attttcctgg
     1201 gccattcgtt acctcgtagc ctttcttacc tctgttacgg aacatagcgg ataaaccttc
     1261 ttctggttca attgcgcgga taagtacgcc ttcaggtata ccttcagatt ttgttacaaa
     1321 attaatgagt aaatgcgtat gcatgacatg tgcataaatt gtaccaccac gtttatataa
     1381 tgacgtgact ttaggtgtta ttttaccgcc atagccatga gccgcacgat cattcaaacc
     1441 taagtaagct tccgtttcca cgatgtagcc tgtatacgtt tgagtggtat cctgataaat
     1501 cactttgaca cctaataagt ttttagcaat ttgtcttgta tcattattaa cgaaatccat
     1561 aatattctcc tcgcaattat atgattaatt taaataaaaa tgaaaaagat atttttattg
     1621 tggtaaatgt ttgataaaat agtattgtta agaattataa aaatgtaaag tatatacttt
     1681 gttatggaat attttatttg catagattga gactctttta aacgtgaaca ttgcagtatg
     1741 acatattttt agtatagtta acgaagcgtt gactttcaaa tgagtctcca tatagatgtg
     1801 tacttgtagc gggttttcat tttactagtg atgaaatata aagttcattt tccaaagtga
     1861 ttgacgattg aaatgaagtg gcttgataat gaatcttgta aattataacg gtaacaataa
     1921 ttaaaaatat aaatatgatt aacgaaaggg agattacaat ggaacaatta cctcaagtag
     1981 gttttatgaa atcattcgtt ttattttgga aaaattatgt aaattttaag gggagatcaa
     2041 gacgtagtga atattggtat atggcactat ggcatttgat acttatggtt ccagctgtgt
     2101 gtgtgctaat aataaaccta tttctattct ttatttctat agcggcggat aacgaaacat
     2161 taatagttat gagtatattg atgattatcc ttacatctgt gtatactttt gtctatagtt
     2221 tggcaacgtt tattccaaat ttagcgttgt gcgttcgaag atttcacgat atatcaagaa
     2281 caatgttatt tccaatgatt atgacagtat tcagtattat attttatata gtattac
//
LOCUS       DANRDI010000255         2273 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.260, whole genome shotgun sequence.
ACCESSION   DANRDI010000255 DANRDI010000000
VERSION     DANRDI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.260"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..705)
                     /locus_tag="PZ377_002761"
     CDS             complement(<1..705)
                     /locus_tag="PZ377_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDM6359605.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESD"
     gene            1112..>2273
                     /locus_tag="PZ377_002762"
     CDS             1112..>2273
                     /locus_tag="PZ377_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDM6359606.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEV"
BASE COUNT          820 a          397 c          360 g          696 t
ORIGIN      
        1 atcagattcg accatacgat caatattatg cttacgcttt ctccagtaat accagagtgt
       61 gaatatacca ataatcgcac caactgctgc tgcaaaagta gcaataccat tggctaataa
      121 aatagagcca tcaaagacat ttagtactaa ataacttccg attaatatga aaatcacgcg
      181 tgcaatttgc tcagttactt ctgacactgc tgttggcccc atagatttat aaccttggaa
      241 tatccctctc catgtcgcta atacaggaat aaagataaca accatactaa tgattcttat
      301 aatccaagta atatcatcga ctgaccaacc gtttttatca tgaatgtttc tagctaatgt
      361 taattcagaa atataaggtg ctaagaaata cagtaccaag aaacctaaaa caccggtaat
      421 actcattaca ataaaactcg atttataaaa tttctgactt actttatatg ccccaatagc
      481 attatatttc gcaacatatt tcgaagctgc taatggtaca cctgctgtcg caactgcaat
      541 tgcaatatta tatggtgcat aagcgtatgt gaacggcgcc atattttctt gtccaccaat
      601 taaatagttg aatggaatga taaaaagtac gcccaatacc ttggtaatta atatactaat
      661 ggtaattaaa aaggttccac gcaccatttc tttactttca ctcattacga atctccctat
      721 ctcatgttta ttaaagtttt gtaaactaaa agctgtttct ctgtaaaatc atttttcatt
      781 attatgaata tatcacaaaa ctttatttca ttgtcgtata ttcaatgaat tatcataaca
      841 aaattatcaa cacattgtca ttgaatacta gattttgatt agaatattac gaaatttcat
      901 ataaacatta tactactatt tgagatgaac atcgcataac agtagaaaaa tcattcttat
      961 catacacata catcttcatt ttttatgaag ttcacattat aaatatattc aacataattg
     1021 tcatctcata acacaagaga tatagcaaag tttaaaaaag tactataaaa tagcaattga
     1081 atgtccagta acaaatttgg aggaagcgta tatgtatcaa acaattatta tcggaggcgg
     1141 acctagcggc ttaatggcgg cagtagctgc aagcgaacaa agtagcagtg tgttactcat
     1201 tgaaaaaaag aaaggtctag gtcgtaaact caaaatatct ggtggcggta gatgtaacgt
     1261 aactaatcga ttaccatatg ctgaaattat taagaacatt cctggaaatg ggaaattttt
     1321 atatagtccc ttttcaattt ttgataatga atccatcata gatttttttg agtctagggg
     1381 tgttaaatta aaagaagaag atcacgggcg tatgtttcca gtttccaaca aagcacaaga
     1441 cgtggttgat acattagtga caactatcga acgccaacat gtaacgatta aagaagaaga
     1501 agctgttagt agaatcgaag ttaatacaga ccaaactttc actgtacata ctcaaaataa
     1561 tagttatgaa agccattcgc tagtgattgc tacaggtggt acaagtgtcc ctcaaactgg
     1621 ttcaactggt gatggttata agttcgcaca agatttaggt cataccatta ctgagttatt
     1681 cccgaccgaa gttccaatta catcagctga acctttcatc aaatccaatc gtctaaaagg
     1741 tttaagttta aaagatgttg aattgtcagt acttaagaaa aatggtaaaa aacacatcag
     1801 tcatcaaatg gatatgttat ttactcattt tggtatcagt ggtccagctg cattaagatg
     1861 tagtcagttt gtttataaag aacaaaaaaa tcaaaagaca cagcacattt ctatggcaat
     1921 cgatgcattt cctgaattaa accatgaaca attaaaacaa cacatcacat cattattatc
     1981 ggacacacca gataaaatca ttaaaaacag tttgcatggt ctaattgaag agcgctactt
     2041 actgttcatg ctggaacaag caggaatcga tgaaaatacc acatcacatc acttatcaaa
     2101 tcaacaattg aacgacttag taaatatgtt taaagggttt gtatttaagg tgaacgggac
     2161 attacctata gataaggcat ttgtcacagg tggtggtgtg tcacttaaag aaattcaacc
     2221 taaaacaatg atgtctaaat tagttccggg attattttta tgtggtgaag tat
//
LOCUS       DANRDI010000256         2252 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.261, whole genome shotgun sequence.
ACCESSION   DANRDI010000256 DANRDI010000000
VERSION     DANRDI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.261"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(1..322)
                     /locus_tag="PZ377_002763"
                     /pseudo
     CDS             complement(1..322)
                     /locus_tag="PZ377_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            324..523
                     /locus_tag="PZ377_002764"
                     /pseudo
     CDS             324..523
                     /locus_tag="PZ377_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399387.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            1129..1479
                     /locus_tag="PZ377_002765"
     CDS             1129..1479
                     /locus_tag="PZ377_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359607.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            1566..1877
                     /locus_tag="PZ377_002766"
     CDS             1566..1877
                     /locus_tag="PZ377_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDM6359608.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            1892..>2252
                     /locus_tag="PZ377_002767"
     CDS             1892..>2252
                     /locus_tag="PZ377_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDM6359609.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKR"
BASE COUNT          794 a          403 c          345 g          710 t
ORIGIN      
        1 tggttagctt tacataattc aataactttt tgtgttacgg tatcattttt atgggttttg
       61 tttttccatc ggtaatatgt tgatttaggt atgtttaata cttttagtag caatttgatt
      121 gaatatttta cttttaattg atccactaaa tctatgacta ctgttggtac cacttccttt
      181 ccaatacctt gtactttttt aaaatatcca attctatatc ttttctctta ttttctaatt
      241 ttaattgttc tacttctgac agctcttcta atcctttacc gtaggtatat tgtttaccaa
      301 cttgttgtga aaatctatat gctagttttg gtaaaactga ggaaggacgc tggatagcta
      361 agaatgccca taattatgga tttattataa gatatcctaa aaacaaagaa agcatcacag
      421 gatatcaata tgaaccttgg catttacgtt atttaggaaa aagaaggcga caaaagtgta
      481 caaatctggt gaaacattag agggatttgt aggcttaaaa tgatcaattt aaaataatcg
      541 aaacttcaac taccttatta tcgacgaagt gtattccagc acttcgtctt tatttttacc
      601 gcaaacatta taccttacta tttaataaat ctattttata tctacatact tcctctttct
      661 ttttctaaaa atttttgaga attctcttta tcaatttcaa taacacttat ctaatgaaac
      721 agtcattgtc gtaatttttt cataactaca cgaggcttat attgaaattg ctaaagaaca
      781 tcccacgatg attttttatc gggaaaggaa tgggatttgc agattagaag aagcagaagg
      841 attgtgcaag cacaatccca aaatcccgtt actttttata aataaataat atatataagg
      901 aagcagagac acaaccctat catcaatact tataaataat gagttgattg tttgtcaaaa
      961 taggcggaat ttcaggattt ccttggcata ccaacgtttg tagcgggttt ttatatggaa
     1021 ttttctctta ttcccaccgg gattcgtaat aaatgacaaa tcaaatatat attatctcaa
     1081 tgatgtatat gaactacatc actattacac tatctaggag gcaataatat gaaaaccatc
     1141 actcaagaac tcaaacaata tataacccac ctattccaac tatctaataa cgaagcttgg
     1201 gaatgtgaag cgctggagga agcagcagaa aatattctac cggaacgttt tattaatgat
     1261 tctccactgg tacatctcac actcgagact tatacctact ataatgatga actacatgag
     1321 ctaagtatct atccatttct aatgtatgcc aataaccaac tcatcagtgt cggatactta
     1381 gaccattttg acatggactt tctgtacctt acagatactc aaaatatcat tattgatgaa
     1441 cgtcatttac taaaacaagg aggtcaggac catgaataat tggatcatag ttgctcaatt
     1501 atccgtcaca gttatcaacg aaatcatcga catcatgaaa gaaaagcaga agggagggaa
     1561 atagcatgaa aatcaatcga tacatcacaa gaggaattaa tgaaagtatt ccactagacc
     1621 ttcaaatctt actttggcac atggtagaaa aaaaagataa ccagcctcat acagattacc
     1681 tacacatttt taaattacaa gaagatgaga atatactctc aatcacacat gaacaagaac
     1741 aacccacata caaattggaa tatcactata caaactatga aaaaaatcaa aatgcattac
     1801 ctaagaaagt ctacgtcatt cgtgaagatg acgtggacgc tttttattat gtgatgcttt
     1861 taccagaaga atactaaagg aagtgacgat gatgaataca atcaaaagta cgatacacac
     1921 agaagccata tttagcgatg atgaacagca ccgctattta ctcaagaaaa catgggatga
     1981 aaagaaagct gcatgcacag tgataacgat gtatcctcat ttagacggtg tattatcact
     2041 cgatctcaca actgttctta tcctcaacca attagccaat tcagaacgat acggtgctgt
     2101 atatcttgta aatctattct ctaatattag aacacccgaa aacctcaaac atatcaaaaa
     2161 tccatacgat gagcacactg atattcattt gatgaaagcg attagtgaaa gtgacacagt
     2221 gattcttgct tatggtgcct atgcgaagcg ac
//
LOCUS       DANRDI010000257         2162 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.262, whole genome shotgun sequence.
ACCESSION   DANRDI010000257 DANRDI010000000
VERSION     DANRDI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.262"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(136..705)
                     /locus_tag="PZ377_002768"
     CDS             complement(136..705)
                     /locus_tag="PZ377_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDM6359610.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            915..1133
                     /locus_tag="PZ377_002769"
     CDS             915..1133
                     /locus_tag="PZ377_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDM6359611.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(1214..>2162)
                     /locus_tag="PZ377_002770"
     CDS             complement(1214..>2162)
                     /locus_tag="PZ377_002770"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDM6359612.1"
                     /translation="LNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQNTIEEVNQT
                     YIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGV
                     NAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNALKVNPAKIKSK
                     GIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWENIEKLSND
                     KYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLE
                     NALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
BASE COUNT          734 a          337 c          295 g          796 t
ORIGIN      
        1 tgtatgtgtt tgaatatcaa gcacaaatat tattataaca agattaagtt aatttgcaat
       61 agatacctta aaaaaatatg cagaacattt acactatatt tataaaacat tccgcatatt
      121 ttaaaaataa acaatttatt tatcttcaaa taaagaatag cgtgtcaatg cttcaaacac
      181 attcaatgtg gctttattat aatctattgg ttggtcggga ttttttgata tcatgcgaga
      241 ttgtttatct accatgtaat agtaaaaaat ggaattgtta tattgatgcc ataaactgtg
      301 gtatgaaaca tgaagaatga ttgattcatt attaataaat gtcactttac atttacgatt
      361 ttttaattct ttaatacttt cgatataatg catatttaac caaatatttt cattttgtct
      421 gtcagaatgt gttgggaaaa aataagttgg aaataatggt gttagtaaaa taggtggttt
      481 actagaaatg ccagtaatgc ggtttgtctc tgctttcttg ccaagataag tattaccata
      541 aaatttgcaa gagcgttcaa tgattttctg gactttaaaa ggattttgaa tacgcgtttt
      601 gtcaaaccga attatttcac taccgtttct ttgatcgtca tcatcaaatg ctggtcgaat
      661 aaccatgtct cctttgcgta tcacataaat attttgagaa tacataagca aaacctcgct
      721 ttattaagtt ttaaaccatt aatataaaaa tacaattaat gatataaaga tattttataa
      781 taaaattctg agttgtcaag ttattatttt ggtgaaaatg tacaataatt tttaactaat
      841 agtcagaata ttcattgatt tcaaaattag aaaaatgtat acttattata aattaaagaa
      901 aggtagtggt ttctatgaaa tacaatacta atgttaaaca tacaacttta gaagcgtttg
      961 tcacaactgt caatgatttg ggtattgaat taattatcaa tgaagcactt cgagaggtaa
     1021 gaaaacgaca gctcatagaa cttatagatg acgcactcgt caataaagat gaagcagcat
     1081 ttaatcaata tacggcagaa tacaaaaatt tggaggcatt tctcggtgaa taacattgat
     1141 tcgaattcca taacgcaatc ataaaaggac tattcttaac atctcataca tgtaagaata
     1201 gtcctttaat cgtttatgac attaatctaa ttaattcatg tctatctatg tcaccgaaat
     1261 aatgatataa atcatattct gatagtgctt cttctatgtg ctcaaagtcg tgtagacagc
     1321 caactaatgc attttcaaga tcagtgacat ctccgacacc aaagaaatca ccgaatattt
     1381 ttgcatgttc gattttacct cgtttaacat caaacttaat ttgtacaaat cctttttcaa
     1441 atttttcctc gcgttcaaag ttatatttag ggtttctacc ataattccat tcccaagttc
     1501 tatatttgtc gttacttaac ttttcaatat tttcccaatc ttcatccgtt aatttatatt
     1561 cttctacttc agtttctcca aagatagttt tcaagatgat ttttttaaat tcttcaattt
     1621 ctaaagggtc atttaaaaat tcttgaatgt ttgctacccg tttacgaaca gatttaatac
     1681 cttttgattt aatcttagct ggattaactt ttagtgcatt ctgaacttcg tctaaatcac
     1741 tatttaacat caacgtacca tgactaaaca ttctattttt aactttaacc atagcattcc
     1801 ctgagatttt cgcttgtcca acttgaatat cgttacgacc agttaattca gcatttacgc
     1861 ctaaagattg taatgcttga acaattggtt cagtgaattt ttggaaattg tggaaactgt
     1921 taccatcatc atctgttata aaactaaagt ttaaattgcc agtatcatga taaacagcgc
     1981 caccaccaga aattcttctt actacatcga tgttgtgagc atcgatatat gtctgattta
     2041 cttcctctat cgtattttga ttctttccaa caatgataga tggtctattt atgtaaaata
     2101 aaaagtaact ttcttctgct ggtaaatttt ttaaaacata ttcttccatt gctaagttta
     2161 aa
//
LOCUS       DANRDI010000258         2160 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.263, whole genome shotgun sequence.
ACCESSION   DANRDI010000258 DANRDI010000000
VERSION     DANRDI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.263"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..540
                     /gene="lpl6"
                     /locus_tag="PZ377_002771"
     CDS             <1..540
                     /gene="lpl6"
                     /locus_tag="PZ377_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HDM6359613.1"
                     /translation="EIVKQPKGKVMKTRGMQLYINRNTETAKGFFVLKEISENNNRVN
                     KDKEEKYEVKMVGNKIIPTEQINDEKIKKEIENFKFFVQYGNFKNFEKYNNGEFSYNP
                     EAPIYSAKYQLHNDDYNVRQLRKRYDISTKETPKLLLKGGGDLKNSSVGQNDIEFTFV
                     ERKGENIYFNDSVEFIPSK"
     gene            610..1386
                     /gene="lpl7"
                     /locus_tag="PZ377_002772"
     CDS             610..1386
                     /gene="lpl7"
                     /locus_tag="PZ377_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HDM6359614.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
     gene            1565..>2160
                     /gene="lpl8"
                     /locus_tag="PZ377_002773"
     CDS             1565..>2160
                     /gene="lpl8"
                     /locus_tag="PZ377_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HDM6359615.1"
                     /translation="MGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFPDRKNYKVEMKNNKIILLDKVEDTNLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKM"
BASE COUNT          962 a          216 c          391 g          591 t
ORIGIN      
        1 gaaatagtta aacagccaaa gggcaaagtg atgaaaacaa gaggtatgca attatatatt
       61 aatagaaata ccgaaacagc caaaggtttc tttgttttga aagaaataag tgaaaataat
      121 aatcgtgtaa ataaagataa ggaggaaaaa tacgaagtga aaatggtagg aaataaaatt
      181 attcctactg aacaaattaa tgacgagaaa ataaaaaaag aaattgaaaa cttcaagttt
      241 tttgtgcaat atggaaactt taaaaatttc gaaaaataca acaatggtga gttttcatat
      301 aatcctgaag caccaattta ttcggctaaa tatcaattac acaacgatga ttacaatgta
      361 aggcaactac gtaaaagata tgacatttca acaaaagaaa caccgaagtt acttttgaaa
      421 ggtggaggag atttaaaaaa ttcctcagtt ggtcaaaacg atattgaatt tacttttgtt
      481 gaaagaaaag gtgagaatat ttattttaac gatagtgttg aattcatacc aagtaagtaa
      541 agttaaatta aaatgtgtgg ttagatgaaa tgatcaaata tcaaaaaatg tgcaaagaga
      601 tgtgacaaga tgaagtgttt tcagaaatta tacatattta tattaatttt aatcgtatta
      661 atggcaggat gcgaaagtaa taagatcact ggagattcga aagaaacaca gatcaaaaag
      721 agctttgcga aaacattaga tgtataccct acgaaaaatc tagaagattt ttatgacaaa
      781 gaaggatatc gagatggcga atttaaaaag ggtgacaaag ggaagtgggt tattagatct
      841 gaaatgacaa cagaactgaa aaatgaaaat atggtatcta aaggtatggt catacgttta
      901 aatagaaata gtagaacatg cactggtgaa tattttgtca ggatagttaa agaagacagt
      961 gagggcaagg tatatagtga tgaacgaaaa tatccagtga aaatggaaaa taataaaatc
     1021 attacattaa aaccaatcga tgatgaaaaa gtaaaaaaag aaattgaaga atttaaattc
     1081 ttcgtacaat acgggaattt caaagaattg gaaaactata aagacggaga agtgacatat
     1141 aacccagaag caccaatata ctctgcacaa tatcaattga aaaatagtga ttataatgta
     1201 gaacaactac gtaagcgata taatataacg acgaaaaaag cgcctaaatt attattgaag
     1261 ggttcaggta atttaaaagg ctcatcagtt ggatataaaa atattgaatt tacctttgtt
     1321 gaaaataagg aagaaaatat ttacttcaca gatagtatta atttcaaccc aagtgaggat
     1381 aaataagtat atttagtatt acaaaaacgt tagaaaaatt aataataaag ttaaattaac
     1441 aaactgtatt aaattcatat agtcagtttt gtttgatgac atgatagtag aaaaattatt
     1501 attttaatag gttgtatgaa ctatacaaat atttataaaa attaaaacag agagatgtga
     1561 aatgatgggg tatataaaaa gaatggcttt atacatgagt gtatttcttt taatcatttt
     1621 tattgttgga tgtcgaaata tgaaagatga acagaaaaaa gaagaacaaa caaataaaac
     1681 agattcaaaa gaagaacaaa tcaaaaagag ttttgagaaa acattagata tgtatccaat
     1741 taagaatctc gaggagttat acgacaaaga aggataccga gatggcgaat ttaaaaaggg
     1801 tgataaaggg atgtggacga tatatacaga tttcgccaaa agtaataaac aaggtggatt
     1861 gagtaatgaa ggtatggtct tatacttaga tagaaataca cggactgcaa agggacatta
     1921 ttttgttaag acattctata ataagggcaa attcccagat agaaaaaatt ataaagttga
     1981 aatgaaaaat aataaaatta tcttattaga taaagtagaa gatacaaatc taaaaaagag
     2041 aatagaaaac tttaaatttt ttggacaata tgcaaacctt aaagaattga aaaactacaa
     2101 caatggtgat gtctcaatta atgagaatgt tccaagttat gacgcaaaat ttaaaatgag
//
LOCUS       DANRDI010000259         2154 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.264, whole genome shotgun sequence.
ACCESSION   DANRDI010000259 DANRDI010000000
VERSION     DANRDI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.264"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..111)
                     /locus_tag="PZ377_002774"
     CDS             complement(<1..111)
                     /locus_tag="PZ377_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDM6359616.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRV"
     gene            complement(115..1524)
                     /gene="sapep"
                     /locus_tag="PZ377_002775"
     CDS             complement(115..1524)
                     /gene="sapep"
                     /locus_tag="PZ377_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDM6359617.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
BASE COUNT          697 a          427 c          310 g          720 t
ORIGIN      
        1 tactcgaatg tattcataaa taccatcgcc aaatacgtat cctctgtcgt tgtatgaaac
       61 ctttgcttca cttggactta caaactcacc atttaaaaaa attttttcca tatattattc
      121 ctccacgcat aatgaataaa ttgcttctaa gtaaatacta gttgcgttaa ataactgttt
      181 tttagtgata tattcatttt tctgatgcat taaatcttca gaatcactaa acattgcgcc
      241 aaatgctaca cccttgtcta agtttctcgc ataagtaccg ccacctatag tataaggttc
      301 agtcatatca tttgtttgat ttctatatgc agtaactaac ttttgtacaa aaggatcatt
      361 tttatcaaca taatgtggtg gttggacttt acctaatttc acttcaaagc catattgttg
      421 aatctcattt gcaaaacgat ccatagcttt ttcaaattca aatccttctg ggtagcgtaa
      481 gttgataccg aaaagacctg cgttttcatt atcatatgta ataacaccaa tgttagttgt
      541 cacgtcaccc atgacatctg tatggaattt cattcccatc ttttcaccaa aatctgaatt
      601 aaataagtag cgattactaa atgctacaaa cgcttgtgca ttattatcaa gatttaatga
      661 tgctaagaat tttagtaagt aaagacccgc attcacaccg atagatggat ccataccatg
      721 aaccgcttta ccttcaactg ttaaaactag aatgccacta tcaacagtac tatcaccttg
      781 taaatgattt tgttctaaaa agtactcaaa gtcttgaata acatctgtca tattttcttt
      841 aacaagcact cttgcttctg catgatcagg taccatgttg taacgttcac cagatttaaa
      901 agttattaat tcataatcag gttcatcttg atcttcagta agtttatttt gaactaaatc
      961 aaatgttgta atgccttttt caccatgaat acatggaaat tctgcatctg gtgcaaaacc
     1021 taatgttggc atttcttctg ttttaaaata gcgatccgta catttccaat cagattcttc
     1081 atccgtacca ataatcatat gaatacgttt cttccaatcc acattcatat cttctaatat
     1141 cttaattgca taataagcag caattgttgg acctttgtca tcaagtgtac ctctagctat
     1201 gatagcatct tctgttacaa ccggctcgaa cggattacta tcccatccat caccagcagg
     1261 aacaacgtca acatgacata agatacctaa tacgtcattt cctttacctg cctcaattct
     1321 tcctgcaata tgatccacat catgtgttgt aaatccatct ctatgtgcaa tttcatacat
     1381 gtagtctaat gccttacgag gacctggacc aactggtgcg tcttctgatg cttttgcatc
     1441 atctctcaca ctttcaattg ctaataatcc ttttaagtca ttaatgattt gatcttcgta
     1501 ttgttgaact ttttctttcc acattcgaaa tcgacttcct tttttctata agttaaattc
     1561 tattttacat gaaaagatat aaaaactaca ataagatgtc agaaaataat aaaaaggaac
     1621 aaaacgatgc tattgatatg acacaaatca taaatagctg ctttgttcct tttttaattt
     1681 atatatttaa aatacacata ttcaagagct cgagatataa gtcaatgtac taggcacaca
     1741 atttaatatt gacagtaatt aaccgaacga aaatgcgccc cggggcccca acatagagaa
     1801 tttcgaaaag aaattctaca gacaatgcaa gttggcgggg ccccaacata gaagctggcc
     1861 aatagttagc tttcaataat gtgcaagttg gggtaagggc cccaacacag aagctggcca
     1921 atagtcagct ttcaataatg tgcaagttgg ggtaagggcc ccaacacaga gaatttcgaa
     1981 aagataaaga aatacgtttt ctttagatat tagtatttct tatgaatgag tttcacgcat
     2041 gtattcttct ttctatatgc atattagcta tgactaacga taaagaacct gaaacactaa
     2101 taaatgtcct atagtttaca atattatatt ggcagtagtt gactgaatga aaat
//
LOCUS       DANRDI010000260         2120 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.265, whole genome shotgun sequence.
ACCESSION   DANRDI010000260 DANRDI010000000
VERSION     DANRDI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.265"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(78..269)
                     /locus_tag="PZ377_002776"
     CDS             complement(78..269)
                     /locus_tag="PZ377_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6359618.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            430..759
                     /locus_tag="PZ377_002777"
     CDS             430..759
                     /locus_tag="PZ377_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6359619.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            772..1233
                     /locus_tag="PZ377_002778"
     CDS             772..1233
                     /locus_tag="PZ377_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDM6359620.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1251..1685
                     /locus_tag="PZ377_002779"
     CDS             1251..1685
                     /locus_tag="PZ377_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359621.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            1714..2109
                     /locus_tag="PZ377_002780"
     CDS             1714..2109
                     /locus_tag="PZ377_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359622.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
BASE COUNT          858 a          322 c          345 g          595 t
ORIGIN      
        1 ttcctccttt taagttgttt atgtccattt gttacattac ctaaaagtta taaaaagtta
       61 tacgaaggtc aaaaaattta gacctttatc tggtctacct caatatcata taactttgct
      121 aaagcgtaaa ctgtaacatt acttaaatta gtgtcatcct tttcccaatg accaactgtc
      181 tttgcattga cgcccaatat atctgctact tcttgctgtt tcaagtccct tacaagtctc
      241 cattttctta cggataattg ttccggcatg tcgtccacct ccctttcaca cgtataactt
      301 tatattactt tgtattactt gtcaacagat aaagttatat ttcttcataa aaagttataa
      361 aaagtattgt aaagtaatgt cggttatggt aagttaatat tacattaagt aatattaaag
      421 gagacaacta tggagaataa taaagtcaga aaaattttat ctgaaaacct tcaagaactt
      481 atgaatgata aaaatattga tcagagagaa cttgctgaag ctattggagt ttctcaacct
      541 acagtctcca attggattca acaaactaaa tatccacgaa ttaaaagaat tcaacaactt
      601 gcagattact tcaatgtacc gaaatcaaga attactgaat caaaaaaaga tatacatcaa
      661 gaaacaattg ctgctcattt tgataaggag ggattaactg aagaagagat tgaagaagta
      721 aatagattca ttgaatgggt tagaaataga gacaaataaa gggtgtttat attgggatta
      781 tacgaagaac tttgcataaa taacgaaaaa ataaagatag aagaaactga ccagcttcca
      841 aatttccaac ctggatgcta tatgaacgga aaaatttata taaggcgtaa tttatcagaa
      901 gtacgtaaag cagaagtgtt atatgagcaa cttgcccacc acaagttgac gtatggcaac
      961 attttagacc aaacaaaatg gattaataga aaatttgaaa attatgcacg tagacatggt
     1021 tttacttcag ctgtaccgct acatgaaatt gtagaagctc acaactacgg tgttcgtaat
     1081 ttgtacgagt tgtcagagta tctgcagttg agtgaatcat acatactaga agctatagaa
     1141 caatataaaa agatatatgg tattggaact cactatggcg agtattctat tacgtttgag
     1201 ccgttgagag tttttaaata taaggaaata taaacaaagg agagatacat atgaaaaaag
     1261 taatcggact gctactagta agtacattag ctttaacagc ttgtggtgaa aaagaaaaac
     1321 caaaaaaaga agaaaataaa aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa
     1381 cgcaacaaga aaaaatgaaa aaagttgaag ataaaaatcc acctaataat agcatacaaa
     1441 ataattcaaa caatcaaaac caatcacaaa acaatcaact taataataat tcagatccat
     1501 ctaataatac tcctgcaaat ataaatgaaa acgattcaca aaatactaat ttaaatgatg
     1561 agtatgtcgt ttcgcctggc tggactaaag atgaacaggc taaagctttt gaagagtaca
     1621 aaaaaggaaa agaagaggaa gcaagagctg gtgctagcgc agtaccagga gccaatatta
     1681 actaataaaa caatataaga aagaagagct aatatggaaa caaataaaac aatcgattta
     1741 atgaattatg tggaatttcc aaaaagatac acagaggcaa aaggcaaatt agttgcacaa
     1801 ccaataacta ctataaatag cgcaagaaga gttgaaaatg aagatatgac tgtttgctac
     1861 attttagatc aggatgatga tgtaatggac tttatctttg acagagatat aattactgtt
     1921 tactgtcctg aaaacggaac tgcgactgat gaatattttt gtgaaattat atttaactca
     1981 gatgacacat ttaccctaaa gcgattatct aattacgtta ccattaaaga tagaagctac
     2041 ccaatgtcaa aaataaatga cgtaaacatt acgggcaaag tcgtcagatt atttagagat
     2101 tttaaataaa cttggcttta
//
LOCUS       DANRDI010000261         2069 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.266, whole genome shotgun sequence.
ACCESSION   DANRDI010000261 DANRDI010000000
VERSION     DANRDI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.266"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..886
                     /locus_tag="PZ377_002781"
     CDS             <1..886
                     /locus_tag="PZ377_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDM6359623.1"
                     /translation="VSLNYPLFNLVDQFTHNGALSLVGIPSQLQDIFFNMLNMSTNKI
                     VMIPTSLSAGFAVSLIPYITKTFAEGRLHEMHHQIRTSIGVLMFITVPASIGIMALAQ
                     PLFTVFYGYDPIVLGHDPNHDGSRLLFYYAPVAILISLLSVTASMLQGIDKQKLTVYV
                     ILASVVIKLALNYPLIMLFHTPGAILSTSIALLFAIGCNFYILKKYAKFKFSYSWIHF
                     AKIFLYSFIMMLGVELVFFLANLFLEPTKLGYLIIIILGVTVGILIYGTITIKTRLAD
                     EFLGEIPEKLRRRVRFLR"
     gene            883..1578
                     /locus_tag="PZ377_002782"
     CDS             883..1578
                     /locus_tag="PZ377_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDM6359624.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            1595..2017
                     /locus_tag="PZ377_002783"
     CDS             1595..2017
                     /locus_tag="PZ377_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDM6359625.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
BASE COUNT          781 a          277 c          349 g          662 t
ORIGIN      
        1 tgtaagtttg aattatccat tatttaactt agtagatcaa tttacacata acggtgcttt
       61 atctttagtt ggtatacctt cacagttaca agatatattc tttaatatgc taaatatgtc
      121 aactaataaa attgtaatga ttccgacatc attaagtgct ggttttgcag tgagtttaat
      181 tccttatatt acaaaaacat ttgcagaagg tcgcttacat gaaatgcatc atcaaattag
      241 aacatctatt ggtgtattaa tgtttataac tgttccagct agtattggaa ttatggcctt
      301 ggcacaacca ttattcacag ttttctatgg ttacgatcca attgttttag gtcatgaccc
      361 taaccatgat ggtagtcgct tattattcta ttatgcgcct gtagcaatac taatttcact
      421 attaagtgta acggcatcaa tgttacaagg tatagataaa cagaaattaa cagtttatgt
      481 tattttagct tctgtagtta tcaagttagc gttaaactat ccattaatta tgttattcca
      541 tacacctggt gcaattttaa gtactagtat cgcattatta tttgcaattg gatgtaattt
      601 ctatattctt aaaaaatatg cgaagtttaa attcagctat agttggattc attttgcaaa
      661 aatattcttg tattcattta ttatgatgct aggtgtagaa ttagtatttt tccttgcaaa
      721 tctattctta gaacctacta aacttggtta cttaatcatt attattttag gtgttacagt
      781 aggtattcta atttacggta caatcacaat taaaacgcgt cttgcggacg aattcttagg
      841 tgaaattcct gaaaaattaa gacgtagagt taggttttta cgatgagaat agataaattt
      901 ttagcaaata tgggcgtcgg tacacgtaat gaggtcaaac agttacttaa aaaaggcctt
      961 gtaaatgtca acgaacaagt aattaaatca ccaaaaacac atatagaacc agaaaacgat
     1021 aagataacag ttcgtggaga attaatagaa tacattgaaa atgtctatat tatgttaaat
     1081 aagccaaaag gttatatatc agcaactgaa gatcatcatt caaaaaccgt tattgattta
     1141 atacctgaat accaacatct aaatattttt ccggttggtc gtcttgataa agatacagaa
     1201 gggcttttat tgataacgaa tgatggtgat tttaatcatg aattaatgag tccaaacaaa
     1261 catgtttcta aaaaatatga agttatttca gcaaatccta tcactgaaga cgacattcaa
     1321 gcttttaaag agggtgtaac actgactgat ggcaaggtta agcctgcaat attaacttat
     1381 attgataatc aaacgtcaca cgtaacaatt tatgaaggaa aatatcatca agttaaacga
     1441 atgtttcata gcattcaaaa tgaggtatta catttaagac gtatcaaaat tgctgattta
     1501 gaattggaca gcaatttaga ttcaggagaa tatcgtttat taactgaaaa tgattttgac
     1561 aaattaaatt ataaataaaa gggagacgat tgatatggca aaaggtaatt tatttaaagc
     1621 gattttaggt ataggtggcg ctgtagcagc tgtacttgtt acacgtaaag atagtcgtga
     1681 caagctgaaa gcagaatata ataaatataa acaagatcct caaagctata aagataatgc
     1741 taaggataaa gcgacgcaat taggaacaat tgctaatgaa acaattaaag aagtaaaaac
     1801 aaatccgaaa gaatatgcta atagattaaa aaataatcca aaagcatttt tcaaagaaga
     1861 aaaatcaaaa tttaccgaat atgacaataa gactgacgaa agtattgaaa aaggtaaatt
     1921 tgatgatgaa ggtggcgcag caccaaataa taatttacgt atcgtcactg aagaagattt
     1981 aaaaaagaat aaaaatgcat tatctgataa agaataagat gtgagtagga cagaaatgat
     2041 aagggagtgg gacagaaatc atattttag
//
LOCUS       DANRDI010000262         2062 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.267, whole genome shotgun sequence.
ACCESSION   DANRDI010000262 DANRDI010000000
VERSION     DANRDI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.267"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(60..1412)
                     /gene="glmU"
                     /locus_tag="PZ377_002784"
     CDS             complement(60..1412)
                     /gene="glmU"
                     /locus_tag="PZ377_002784"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDM6359626.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            complement(1435..1566)
                     /locus_tag="PZ377_002785"
     CDS             complement(1435..1566)
                     /locus_tag="PZ377_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359627.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            complement(1756..2058)
                     /gene="spoVG"
                     /locus_tag="PZ377_002786"
     CDS             complement(1756..2058)
                     /gene="spoVG"
                     /locus_tag="PZ377_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDM6359628.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
BASE COUNT          595 a          457 c          288 g          722 t
ORIGIN      
        1 ttacattaat attacatagg attatccttt tatcctagcc attttaaata cgtaaatgat
       61 tatttcctat atccttcttt tgttgtttgt cttgctcttg ccacagctaa actgtcattt
      121 ggtacgtcat ctgtgattgt ggaaccagct gccaccaata catcatcacc aattgttaca
      181 ggtgctacta aattaacatt gcaacctaca aatgaatctt tgccgacgat agttttaaat
      241 ttattttcac catcatagtt aactgtaatc gttccgcaac caatattagt acgttcgcca
      301 attacagcat cgccaatata acttaaatgt gaaaccttgg caccatcttt aagatctgct
      361 tttttaattt ctacaaaatt tccaacctta acatctgcac ctaattgcgc gcctggtctc
      421 aattgcgcaa acggtccgac cttagtattc gctcctacgc tagcatcatt aacaacagac
      481 tgttgaatac atgcaccatt ttcaatcgta ctattgttaa tttcagagta ctgaccaata
      541 acaacatctt cgccaatttc tgtacgacca ttaattcgta cgcctggttc aatgactgta
      601 tcactaccaa ttgtaacgtc tggaccaata taagtgctgt caggatcgat gattgtcaca
      661 ccatttagca tgtgataatg attcgtacga cgttgcatcg ccttctcagc ctgactaagc
      721 attacacgat catttacacc catgatttct tcaacatcat tggtacgata gacttctacg
      781 atgccgccat catttaaaat taacgacaat acatcaggga ggtaatattc accttgcgca
      841 ttatcatttt tcacttgtgt taatttttca aacaacgttt tattattaaa cgcaaaaata
      901 cctgaactaa tttcattaat atccttttca gcttgcgttg catctttctc ttcaactatg
      961 cgttctaaac gacctgacgc atttcgaacg attcttccgt atccatatgg ttgttgaatc
     1021 gatgcagata atacagttgc ttgagcatta gcatcctcgt gatgcgcaat caatgtttct
     1081 aatgtttcct ttgtgatgag cggtgtgtca ccacatacaa cgattgtcgt accttccttg
     1141 tcttctaagt gtgatttcgc catttgcact gcatgcgcag taccgagttg ttcctcttga
     1201 aaactgtata aagaacgctc gcctaaatgt ccttttacac tttcagcacc atgtcctacg
     1261 atggttacaa cttgatcgac accagagcct ttcacacttt ccaatacatg ttcgaccata
     1321 ggtttcccag caacctcgtg tagcacttta tactttttag atttcattct tgtgccttta
     1381 cctgctgcca aaattatcgc gtgtcttcgc atgaacatta acccccatta aattttacac
     1441 ttcgtcatta ttataattta atctgcaata ctctttcaac atataaaagg cattgttttt
     1501 aattcgttgt aattttaaaa acaacttttt tcttacttca aaaatacgag catcattcaa
     1561 acgcaatttt tatcagcaat gaatcaataa ttttcatatc atcataatct gttaacgtat
     1621 atatttatta gcctttcatt aaaaataaaa aaacattaat acaaactaaa aaaactgccg
     1681 acaaagcttt atgctttatc gacagtatca aatcaactta ttgcaaatgt attacatcgc
     1741 taaatataaa attgattaag cttcttctga atcttctgat gtagcgtttt tatctggtac
     1801 tacttcatct gtttcatcat atactttcat cactgcatct tgaatttctt gtctcatatc
     1861 tgaattaata ggatgcgcga tgtcgcggaa ttcaccatct ggtgtacgtt tacttggcat
     1921 tgcaacgaac aagccagagt ttccttcaat tacacgtaaa tcatgaatta cgaaagcttc
     1981 atctaatgta atggaaacga gtgctttcat tcgtccatct gtttgtattt ttctaagtct
     2041 tacatctgtc actttcatgt ag
//
LOCUS       DANRDI010000263         2046 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.268, whole genome shotgun sequence.
ACCESSION   DANRDI010000263 DANRDI010000000
VERSION     DANRDI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.268"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(92..1060)
                     /locus_tag="PZ377_002787"
     CDS             complement(92..1060)
                     /locus_tag="PZ377_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDM6359629.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYF
                     SEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLW
                     ILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHM
                     FRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLN
                     KHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
     gene            complement(1337..1489)
                     /locus_tag="PZ377_002788"
                     /pseudo
     CDS             complement(1337..1489)
                     /locus_tag="PZ377_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(1489..1980)
                     /locus_tag="PZ377_002789"
     CDS             complement(1489..1980)
                     /locus_tag="PZ377_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDM6359630.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
BASE COUNT          667 a          352 c          227 g          800 t
ORIGIN      
        1 atacacactc atccctcact gcaacacagg gcgtttctca gcgtcttaaa ataaaaaaac
       61 gccactcgta agtgacatta aaaaatatct tttataacat atccagtatt tttgtttgat
      121 ctggaaaatt catttttgta tataggtcat ctatagtaat tgtatagaaa gaatgtgaat
      181 aattacttat tagtttatcc atatttttca tcaattttat gtaatcatgt ttattcaaaa
      241 atagacatag tgaaatcaac aaatcgaata cataactacc attcattgta ggaacgtatt
      301 ctttatcgta tattttgtta taattggcga atatattagc tctactttta gccctgtcta
      361 ttttataatc atacaatctt tcttcatgcg cacacacgtt acgaaacata tgtgcttgtt
      421 gtagtatact gtctacatct gatggagtta tttgaacacg tgttttataa tctctttcca
      481 atttcctttt atagtcttta gcaacttcca atcgaagatc atcatccaaa ttggaataca
      541 tttttgaaac attacctaaa gttaaataat tcaccaatat ccatagtggc actccattat
      601 gagtattaat ataatgtttt aatggtttat tttttctatt actcataacc gagctaaatg
      661 tagcaaccat tttcacgatg ctatctgtct tacttgtgtc agatgaataa tttttaaagt
      721 ataaatatga atgtggttct ctatattttt cgctaaaata atatgaaatt cttgatttaa
      781 tatgagtttc aaatactaac aaatactcta ataaaacatt cctaaatttt ctatctagtt
      841 tgtataaaga aaagacttct ttaaaatgag taccttgctt atatttatca ggaaccaaaa
      901 aattaccatt aacatctagt tctaaaaata aatctttata accatttatg atattataat
      961 aattttcatt ttctaaatct cttttagcac tactcggtac ttccattcct cttcttctta
     1021 gaattttcaa ttgtttatta tgactttcaa atggcttcat aaatttaccc ctataaaagt
     1081 agccataacc cgaatagagt tatggctaga tcgttatata tataatacat ttaattttat
     1141 cggttgtaaa taaataagaa ataacaaaaa gagatatttt acacaattac ctcctttatt
     1201 ttattgctac tcctcaaacc caccaatatt atcaataaac actggtgttg ttacatttaa
     1261 gtctactttc tcagtaaata agctatggta tcaaactaat tagtattaat ttataaatag
     1321 catagcttca ttttcttcaa tcctctaacg gtatatcatc cacaatcaca gtatgattat
     1381 ggttagcgtt agatacatct tgcactgcct tatctaattc ctcatcatct ccgtcccatt
     1441 caccaatatt aatgaatata ggaatattcc cgttaatatc atgcttatct atgaaagttc
     1501 ttttttaact tgttctaaat gcttattaaa ctctattcga tgtaattctt ctcgctggtt
     1561 ttgtcgttct tgacgctttc tactttcaac tttataaata tctactttta aacgttcaat
     1621 gactccttca tttttaactt tcccactact taacatgtac ctcataacat cctgttgttc
     1681 attttcagaa tagtttttga tgatattttt aaacaaattt agcctatcct gagctgtttt
     1741 catgtaatat tcaagtttat ctttctcttc tataatataa agtactaatt tatctaatgg
     1801 atatgataca gataccagca tatcgttatc attcgtgatc acatggttca tgttcaagtg
     1861 atataagctt tctatttgtg atctcatcgc tttaattttg ctattaatga ctttcggatt
     1921 gtaatgcatt aaaagttcta cttcagtgat tttatcattg ctttcaaagt cagataccaa
     1981 ataaccacac ctttacatat cgaatttaat atttataagt atctgaaaaa gggataaatc
     2041 aagtta
//
LOCUS       DANRDI010000264         2038 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.269, whole genome shotgun sequence.
ACCESSION   DANRDI010000264 DANRDI010000000
VERSION     DANRDI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.269"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            152..502
                     /locus_tag="PZ377_002790"
     CDS             152..502
                     /locus_tag="PZ377_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDM6359631.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            495..1058
                     /locus_tag="PZ377_002791"
     CDS             495..1058
                     /locus_tag="PZ377_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDM6359632.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTFDIVTFDELTAFINDLQWSEDQSF"
     gene            1072..1416
                     /gene="gpsB"
                     /locus_tag="PZ377_002792"
     CDS             1072..1416
                     /gene="gpsB"
                     /locus_tag="PZ377_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDM6359633.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            1479..1858
                     /gene="rnpB"
                     /locus_tag="PZ377_002793"
     ncRNA           1479..1858
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PZ377_002793"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
BASE COUNT          786 a          263 c          369 g          620 t
ORIGIN      
        1 taaaattttg tatttatata ggaagttttt agatttactt gatttaacag ataacacatt
       61 tgaaaaaata gttaaatcaa attatataga gtgttaaaac atgacgaata cagtatattg
      121 tgataaatta aaaaatgtag gaggcgtcct catgaatgat gtagtcgaat cactaattta
      181 tgaagttaac aacatgcaac aaaattttga aaatgtgaaa tcacaacaac aagatcatga
      241 tttttaccaa actgtaaagc catatactga acatattgat agcatactca atgagatcaa
      301 attacatcgt gaatttatta tagaagtacc ttatatgaat tcaaggaaat ttgagctact
      361 gattgctaac attgaacaac tttctgtcga atgtcatttt aagcgaacaa gtcgaaagtt
      421 atttatagaa aagcttaaaa gtgttcaata tgatttacaa aatatattag atggcgtaac
      481 aaaagagggt actgatggtt aaaacagttt atgtaacagg ttacaaatca ttcgaattaa
      541 acatttttaa agatgacgca cctgaagtac attatttaaa acaatttata aaacataaaa
      601 ttgaacaact gttggatgaa ggattagaat gggtgttaat acaagggcaa atgggtatag
      661 aattatggac tgcagaagtt gttattgaat tacaacgaac atatgattcg ttaaagtttg
      721 ctgtaattac accgttccaa ggacatacag aaaagtggaa tgaacataat caaagtaagt
      781 atgccaacat aattaagcat gcagattatg ttgatagtat ttttcatacg tcgtatcaag
      841 gtccttttca atttaaacaa gcagatcaat ttatgcttga acattcggat caaacattgc
      901 tcatttatga tgaggaacaa gaggcgagtc ctaagttctt caagcagatg ttagttgatt
      961 ttatggataa aacaaactat acttttgata ttgtgacgtt cgatgaatta acagcattca
     1021 tcaatgactt acagtggtct gaagatcaaa gtttctaatg aggtggaaaa aatgtcagat
     1081 gtttcattga aattatcagc aaaagatatt tatgaaaaag attttgaaaa aacgatggct
     1141 cgtggctata gaagagaaga agtagatgca tttttagatg acattattgc tgattatcaa
     1201 aaaatggccg atatgaataa tgaagttgta aaattatcag aagagaatca taaacttaaa
     1261 aaagaattag aagaattaag actacgtgta gcaacatcaa gacctcagga caataaaagt
     1321 ttttcttcga ataatacaac aacaaataca tcttcaaata atgtagatat tttaaaacgt
     1381 atttcaaact tagaaaaagc tgtatttggt aaataattac taaatacaaa agtttaactg
     1441 tcttactaat aatgactatg ttataatttt aaaagtgata ttttgggtaa tcgctatatt
     1501 atatagagga aagtccatgc tcacacagtc tgagatgatt gtagtgttcg tgcttgatga
     1561 aacaataaat caaggcatta atttgacggc aatgaaatat cctaagtctt tcgatatgga
     1621 tagagtaatt tgaaagtgcc acagtgacgt agcttttata gaaatataaa aggtggaacg
     1681 cggtaaaccc ctcgagtgag caatccaaat ttggtaggag cacttgttta acggaattca
     1741 acgtataaac gagacacact tcgcgaaatg aagtggtgta gacagatggt tatcacctga
     1801 gtaccagtgt gactagtgca cgtgatgagt acgatggaac agaacatggc ttatagaaat
     1861 atcactacta gtttagctct cctagatgat ggagagcttt tttcatgaaa agaacactta
     1921 aaattaacgc cttgtcttga tataatgaca ctgccttgtt ttaaaatagt aagcggatgc
     1981 gttaatgtat cagcgattaa atttgttgga aatgtataaa aaacacaagc taagaata
//
LOCUS       DANRDI010000265         2036 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.270, whole genome shotgun sequence.
ACCESSION   DANRDI010000265 DANRDI010000000
VERSION     DANRDI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.270"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>2036
                     /locus_tag="PZ377_002794"
     CDS             <1..>2036
                     /locus_tag="PZ377_002794"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDM6359634.1"
                     /translation="TITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAI
                     QCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTH
                     AISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFNI
                     QPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDE
                     VGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLE
                     MWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFV
                     QESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTL
                     EYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNG
                     LLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDIEGMESLSHY
                     WSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFDEVKDMYRRV
                     NFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVSFFKGEIGQP
                     VNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTEQDIIS"
BASE COUNT          704 a          322 c          403 g          607 t
ORIGIN      
        1 acaattacag agatggtaac aggaattgat attgttaaga cacaaatttt agttgcagca
       61 ggtgccgatt tatttggtga agagattaat atgccgcaac aaaaagatat tacaacatta
      121 ggctatgcca tccaatgtcg tattacaaca gaagatccgt taaatgattt catgccggat
      181 actggaacaa tcattgctta tcgttcaagc gggggctttg gtgtacgtct tgatgctgga
      241 gatggtttcc aaggtgctga gatatcacct tattatgatt cattactcgt aaaattatct
      301 acacacgcga tatcatttaa acaagcagaa gaaaaaatgg tacgctcatt acgagaaatg
      361 cgtattcgtg gtgttaaaac taatattcca ttcttaatta atgtaatgaa gaataaaaag
      421 ttcacaagtg gtgattacac aactaaattt attgaagaaa caccagaact tttcaacatt
      481 cagccgtctc tagatagagg tactaaaaca ttagaatata taggtaatgt aacaattaat
      541 ggtttcccaa atgttgagaa acgtccgaaa ccagactatg aattagcatc aattccaact
      601 gtatcttcaa gtaaaatcgc ttcatttagt ggtacgaaac aattgcttga tgaagtaggt
      661 ccaaaaggtg tagctgaatg ggttaaaaag caggatgatg tcttactaac agatacaacc
      721 tttagagatg cacaccaatc attattagct acacgagtta gaactaagga tatgattaat
      781 atcgcatcca aaacagcgga cgtatttaaa gatggtttct cactagaaat gtggggcggt
      841 gctacatttg atgtggcata taatttcttg aaggaaaacc catgggaacg acttgaacgt
      901 ctacgtaaag ctattccaaa tgtattattc caaatgttgt tacgtgcttc aaacgcagtt
      961 ggttataaaa actatcctga taatgttatt cataaattcg tacaagaaag tgctaaagca
     1021 ggcatagatg tctttagaat tttcgattca ttaaactggg tagatcaaat gaaagttgcc
     1081 aatgaagcag tacaagaagc gggcaaaatc tcagaaggta ctatttgtta tacaggtgac
     1141 attttaaatc ctgagcgatc aaacatttat actttagagt attatgtcaa actagctaaa
     1201 gagttagaac gtgaaggttt ccatatttta gcgattaaag atatggcagg cttattaaaa
     1261 cctaaagccg cttacgaatt gattggtgag ttaaaatcag ctgtagattt accaattcat
     1321 cttcacactc atgatacaag tggtaatggt ttattaacat acaaacaagc aatagatgct
     1381 ggtgtcgata tcattgatac tgctgttgct tcaatgagtg gtttaacaag tcagccaagc
     1441 gccaattcgt tatattatgc attaaatggc ttcccacgcc accttagaac tgatattgaa
     1501 ggtatggagt cacttagtca ttattggtca actgtacgta cttattattc agactttgaa
     1561 agtgatatca aatcaccgaa tactgaaatt tatcaacatg aaatgcctgg tggacagtat
     1621 tcgaatttaa gtcaacaagc taaaagttta ggtttaggcg aaagatttga tgaagtcaaa
     1681 gatatgtatc gcagagtgaa tttcttattt ggtgatatcg taaaagtaac accatcgtct
     1741 aaagtagttg gtgatatggc actttatatg gtacaaaatg atcttgatga acaatccgtg
     1801 attacagatg gctataaatt agatttccca gaatcagtag tgtcgttctt caaaggtgaa
     1861 ataggacaac ctgtaaatgg ttttaataaa gatttacaag cggttatttt aaaaggccaa
     1921 gaagcactaa cagctcgtcc aggtgaatat ctagagccag ttgattttga aaaagtccgt
     1981 gagttgcttg aagaagagca acaaggtcct gttacggagc aagatattat tagtta
//
LOCUS       DANRDI010000266         2022 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.271, whole genome shotgun sequence.
ACCESSION   DANRDI010000266 DANRDI010000000
VERSION     DANRDI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.271"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..1108)
                     /locus_tag="PZ377_002795"
     CDS             complement(<1..1108)
                     /locus_tag="PZ377_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDM6359635.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRV"
     gene            1234..1848
                     /locus_tag="PZ377_002796"
     CDS             1234..1848
                     /locus_tag="PZ377_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDM6359636.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            complement(1845..1991)
                     /locus_tag="PZ377_002797"
     CDS             complement(1845..1991)
                     /locus_tag="PZ377_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359637.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
BASE COUNT          591 a          282 c          297 g          852 t
ORIGIN      
        1 ctaccctttt cataattgct tttaaagaaa cattcatttc tactaataaa gttatgtgtg
       61 tatgtctaaa tgtgtgcgtg gtaactttct tattcatatt taaagctttt gtagttttct
      121 taagcacacc ggcgatttga ttattacata aaggattccc tttttttgtt gtgaatatga
      181 accctctgtc aacatagctc gaattccatc ttttcaacat tttgttttcc agtattatct
      241 ttttaaaaat ttctacggtt ctagaattga tgctgatact tctttttgaa cttatagtct
      301 ttgtagtgtc tttgtatccg aatccttcct cgtatttaat gcggtgaatt gtacctgtta
      361 tattgatagt tttgtttaat aaatctatat ctttttcctg cagtgcttgt agttctccta
      421 tgcgcatacc agttaaagcc tgtacttcta agatgctggc aattaaaatg cgatttcgct
      481 tgtgtaactt attatcattt agtatatgat cacgtatctg taggacttgg ttcatttcta
      541 aatagttgta cattttagat tcatcttttt cgatatcctc tattgttttt cttcttttag
      601 gaattttgac attagttaac aaatattcat ttggataatt gtaaaattta actgcatatt
      661 taatagctcc tttcatatct ccgagttgac gggttacttg attttgagaa tagatatctg
      721 ataatttatt aataaatatc tgcatatatt ttgtatctag tttgtttaaa agcaagttct
      781 cagagctgta tcgtttaatg tttctaattc ttatttttat attattaaga gtagtcaact
      841 ttgaacctga tgtttttata tgatattcaa gccattcatc taatagcgcg tgaaaagtca
      901 aagtttttaa ttcgcttgac gacttgttgt tcagtttttc ttttattttt tcttctaaac
      961 gaaacattgc ttctttttga gattgttttg tattcttgtt caacacaaca cttacgcgct
     1021 tccatttatc tgtgtatgga tctttatact tctcgtagta tctgtattta gtttcgttat
     1081 ttttgttttt aaatttttca atccacatgt ttatacctcc tgcaagaacg tatgttctat
     1141 aaaatattaa aaaataataa gggtagtcgg gctacccgta atttagtact aggtactaaa
     1201 tgtgttataa taaaataaaa agtaggtgat aagatgactc aatttctagg ggcgcttctt
     1261 cttacaggag ttttaggtta cataccatat aaatatctaa caatgatagg tttagttagt
     1321 gaaaaaaaca aggttatcaa tactcctgta ttattgattt tttctattga aacatgtttg
     1381 atatggtttt atagttttat aatttttaat aatgttgatt taaaaaattt gaatttaatt
     1441 cagttgctta caggtctaaa agcaaatatt ttgtttctat ttatttttgt tttaacagtg
     1501 tttgtattta atcctttaat tgttaaattt attatctggt taattaatat aaccagaaag
     1561 tttatgaaat tggattgtat aagcttatta gacaaaagag acaagttgtt taataacaac
     1621 ggtaaaccag tatttatagt tataaaagac tttgaaaaca gaatcattga agagggtgaa
     1681 cttaaaacct ataattcagc tggtagcgat ttcgatttac tagaagttga gcgacaagat
     1741 ttcaaagtat ctgatttacc gtcaaacgat gaattgtata ttaaacatac gcttgtagac
     1801 cttaaacaac aaattaaatt ggatttatat ttaatgaatg aatactaatc ttttttctta
     1861 gctttttctg ataaagtgct ttttaatttt tcgctggcgc ctgacttttc aaaacttttg
     1921 tttaatgggt tactacgagt agtttcttgt tttttgtttt tatctaccat aaaattctca
     1981 ccaccattca acgtctacac tagtaggcgt tttttgattt tt
//
LOCUS       DANRDI010000267         1975 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.272, whole genome shotgun sequence.
ACCESSION   DANRDI010000267 DANRDI010000000
VERSION     DANRDI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.272"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(316..1233)
                     /locus_tag="PZ377_002798"
     CDS             complement(316..1233)
                     /locus_tag="PZ377_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDM6359638.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(1233..1724)
                     /gene="lspA"
                     /locus_tag="PZ377_002799"
     CDS             complement(1233..1724)
                     /gene="lspA"
                     /locus_tag="PZ377_002799"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDM6359639.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
BASE COUNT          689 a          343 c          246 g          697 t
ORIGIN      
        1 ttacatagaa gaatcgtagt attactactt attcttgcat atgtgaaagt gttatgatca
       61 ttttagtata tatctatatt ttcatgtctt tgacgtctct ctggacgctc aattaaagac
      121 tctttatgtt gtacttaaac tgttataagg ataatactaa tcaggcgctt tgtcaatcta
      181 tattgaaaaa ttaaccatac tcagtaaaaa tgttaacctt aatctatttt ataatgtgtt
      241 ttcatttaaa aaaacgaaaa cgataaacac ttatagttta cataataatg ttatcgtaat
      301 tatagcacga cacaattatg catctctttt tcgaattgta tctaataaat cttcaaagtc
      361 ttgtggtaat tcagcatgtc tttcaatata ttcacttgtt actggatgtt cgaatccaat
      421 aagtccagca tgtagagctt gaccaccaat atccaatgtc tttttcggtc catactttgg
      481 atcaccaact aatgggaagc caatatattt catgtgaaca cggatttgat gcgtacgtcc
      541 tgtttcaagt tgacattcaa caagcgtata atctttaaaa tgttctagta cgttaaaatg
      601 tgtcactgct tccttaccat catcaacaac agccatagat tgacgatcat ttttgtttct
      661 accaattggc gcatcgattg taccgtaatc atgaggaata ttcccgtgaa ctaaagcgat
      721 atattttctt ttaacagatt tatccattaa ttgttctaca agcccacgat gagcaatatc
      781 atttttagca accattaata aaccagaagt atccatatct atacggtgaa caatacctgg
      841 acgaatttct ccattaatac ctgacaaatt tttaatttga tacattaaac cattaactaa
      901 tgtattggta taatgccctg gtgatggatg aactaccatg cctttcggtt tatatacaac
      961 tgcaacatcg tcatcttcat aataaatatc taaatttaaa ttttcaggta gaatatcagc
     1021 ttcaaccact tctttttcag tgacaactat atgatcatta agtttcactt tataattaga
     1081 tttaacaact ttatcgtttg caacgactaa acctgcttta atccaatctt gtatctggtt
     1141 acgagaccaa tcattattta attcaggcag caacttatct acacgcatac ctgtttgttc
     1201 tttatctgta atgttaaatt cataagtctc cattacttaa cctccttctc ctttttattg
     1261 gaagtatcct ttaataaggc aataataatt aatattacac caattgttaa acttgaatct
     1321 gcgatattaa atattggaaa atcataacca aaaatatttg tatcaataaa gtcaacaact
     1381 tctcctgtta aaattctatc aataaagttt ccaagtgcac ctgcaaaaag taaactaata
     1441 gcaacttgca taaacaaatt atattgagca tctttaataa agaaatatac taaggctatt
     1501 aatataataa tggtaataat aaagaaaaat gtcatttttc cactcaatat tccccatgca
     1561 gcaccattat ttcgatgtga tgttatgttt aaaaagtgcg gtatcacttc aaatgaatct
     1621 ccaattttca ttgtagtagc tataatatat ttagtaactt ggtcaaatat aacgacaaat
     1681 actgctatta aaatggaagt gccaataaaa tattttttgt gcattttcgt tcctccaatc
     1741 aatcgtccat gagacaactc tttatattat agcttacacc tgctaataaa aaaagtaagc
     1801 atattacatt aaatctaatg ttactaactc aatacttgat aaactactat gttttgacat
     1861 taaatatgaa cttaattatt catttatcat atttaagatg acattaaaaa ttaggaaagc
     1921 aggctggaac ataaatccct aaaaagacag tagtaagata ttttctaatt aaaaa
//
LOCUS       DANRDI010000268         1919 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.273, whole genome shotgun sequence.
ACCESSION   DANRDI010000268 DANRDI010000000
VERSION     DANRDI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.273"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..72
                     /locus_tag="PZ377_002800"
     CDS             <1..72
                     /locus_tag="PZ377_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359640.1"
                     /translation="EELETYIKQQGLEYEEQKQLTLF"
     gene            81..452
                     /locus_tag="PZ377_002801"
     CDS             81..452
                     /locus_tag="PZ377_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDM6359641.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            445..681
                     /locus_tag="PZ377_002802"
     CDS             445..681
                     /locus_tag="PZ377_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDM6359642.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            671..913
                     /locus_tag="PZ377_002803"
     CDS             671..913
                     /locus_tag="PZ377_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359643.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            906..1439
                     /locus_tag="PZ377_002804"
     CDS             906..1439
                     /locus_tag="PZ377_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HDM6359644.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            1476..1721
                     /locus_tag="PZ377_002805"
     CDS             1476..1721
                     /locus_tag="PZ377_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359645.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            1718..>1919
                     /locus_tag="PZ377_002806"
     CDS             1718..>1919
                     /locus_tag="PZ377_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDM6359646.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECG"
BASE COUNT          729 a          248 c          465 g          477 t
ORIGIN      
        1 gaagagcttg aaacatatat aaagcaacag ggtttggaat atgaggaaca gaagcaacta
       61 actttatttt agaggaggtt atgaaagtga actatgaaac aggattccaa ataggcgtaa
      121 tggaagctag gttgaagaag atgagaaaac aacgtgatga gtacaagaag caacgtgacg
      181 agcttattgg ggatatagct aagttaagag agcgtaacga agagctggag aacatgtggc
      241 gcacgcttaa aaatgaattg tttggaagat acgaatttta ccgttttaga cttagcgaac
      301 tacagattga gagcagagcg aacaaggaag tagctatata tagaagagct gaaatcaact
      361 taagtgttat attgtgccga atggacaaac tagacggaac aaatgagttc tacgaatttt
      421 tagggcaaat ggaggatgac acaaatgaat aaccgcgaac aaatcgaaca atcagttatc
      481 agtgctagcg cgtataacgg caatgacaca gagggattgc taaaagagat tgaagacgtg
      541 tataagaaag cgcaagcgtt tgatgaaatt cgcgaagcta ttaatgcgca atgggttgag
      601 tatccagaag actgggcgtc agaggttttg agagaagtaa gagagcttga atatgaggag
      661 gaacaggaaa atgaaaatta atgaatcttt gaagaaatta aaagaaaaag gatacaaaga
      721 aaatgaagat aaagctattt ttaatttagc tgatggtacg ctagaaattt acatcgacca
      781 tgacgaaaaa acaataatta cagaattcca tgatttaaag gtattcgtgt cagaagattt
      841 aaaagataaa agtatggaga gcgttatgta tgaattggct ggaattgatg aggaggacaa
      901 ggaaaatgac taacatccta caagtaaagc tattatcaga aaacgctaga atgccagaac
      961 gaaatcataa gacggatgca ggttatgaca tattctcagc tgaaactgtc gtacttgagc
     1021 cacaagaaaa ggcagtgatc aaaacagatg tagctgtaag cataccagag ggctatgtcg
     1081 gactattaac tagccgtagt ggtgtaagta gtaaaacgca tttagtgatt gaaacaggca
     1141 agatagacgc gggatatcac ggcaatttag ggattaatat caagaatgat aatgaaacgt
     1201 tagagagtga ggatatgagt aactttggtc ggagtcctgc tgggatagat ggaaagtatg
     1261 cccgactacc tgtaacagat aaaattttat gtatgaatgg tagttatgtc ataaacaaag
     1321 gcgacaaact agctcaattg gttattgtgc ctatatggac acctgaacta aagcaagtgg
     1381 aggaattcga gagtgtttca gaacgtggag caaaaggctt cggaagtagc ggagtgtaaa
     1441 gacatattag atcgagtcaa ggaggttttg gggaagtgag cgacatgtta gaaatatttt
     1501 taatagggtt tggcgtttat ctcttttatc gcatagcaat tatttttctt aagagtaaaa
     1561 agactataca cacaaacata tatgaaatgt taatgcttgc tactatcttt atgatatcta
     1621 catttgctta taaacatcaa aagacgcata tcttaatagc atttttagta atgtttttta
     1681 tgagtaagct caaacaagtt caagggagct atgaggaatg acacaatact tagtcacaac
     1741 attcaaagat tcaacaggac gtaaacatac acacataact aaagctaaga gtaatcaaag
     1801 gtttacagtt gttgaggcag agagtaaaga agaagcgaaa gagaagtacg agaaacaagt
     1861 taaaagggat gcagttatta aagtgggtca gttgtttgaa aatataaggg agtgtggga
//
LOCUS       DANRDI010000269         1903 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.274, whole genome shotgun sequence.
ACCESSION   DANRDI010000269 DANRDI010000000
VERSION     DANRDI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.274"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(893..1087)
                     /locus_tag="PZ377_002807"
     CDS             complement(893..1087)
                     /locus_tag="PZ377_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359647.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            1197..1889
                     /locus_tag="PZ377_002808"
     CDS             1197..1889
                     /locus_tag="PZ377_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359648.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
BASE COUNT          778 a          283 c          262 g          580 t
ORIGIN      
        1 cgtgtctatg cgtagacatg gagacacttc agaattatgg agtgattaca tgagtttaac
       61 tatttttctt tattcagttt taggaagttt attagcaaat tatatctacg aaaaatcttc
      121 aaaataaata actttttcgg ttctactttg tacactttat ctacctggta gacctaattg
      181 ttattgcttt acaggtctaa aattcgtcgt tataatctag ttaaatcccg taagggcgca
      241 cttatacgtc ggataaatcg acatgtgcaa aaacgtaatc gaccttcgtc gattttttta
      301 tttcaaaatt aaagaaacgg cttgagatat tccgagcagt tataaaaatt aatttgcgtt
      361 gaatattgct actacattct tgaaaaagtg atgtagattt atagcccata acttttttga
      421 attacatcga cgatacggcg tcgaaagaaa aataaataga ccgtatgaaa gaaaaagcta
      481 cctcacttta tctggggtag cttttatttt ttatgccctt attttgaatt ttaagagggc
      541 attttaaaga tttaggggtg attcatatag ttttatatct aaaacttata tatgctttta
      601 aaacgcaaat atgagccaaa taaatatatg ctattttcaa aaacaaaaat ttgagcaaaa
      661 acaataacta tttttagaca ctgcccattt acatgcaaat taaaaattag cataaaattc
      721 tttaaaactt ccccacgttt ttctaaattc ctgtagggcg tttttatcaa aaatcaagac
      781 aagaagaaac tcgttcacac tcgtttcaaa aaaacaagaa atctaatcta tacacctacc
      841 gttttccaac cccctcaaag catacaaatt aaaaacaaaa ttattcataa tcttactttc
      901 ttttaacaaa aacataaaaa ccataaatag acaaattaac tatcaaaggc aataaacagt
      961 ataaaaaaac cacgaataaa tcttcatcaa catctaaaat aaaaaagaat aaaacaatta
     1021 acaaaatact aattaacaaa cctaaaagtg gcaaactttt tatatatttt tctaaatttt
     1081 cattcataaa atagactcct ttcaaaaata tgtttataaa tatattaaaa taaatattga
     1141 ttttataaag cgcttactta tacaattaaa atatcttatt atcttggagg tatcaaatga
     1201 aaattataaa attattttca ataatttcct tgttcctact ggtatttagc gcgaattttt
     1261 ctaatgctta cgcaaatgaa gaacaaaaaa gttctttact agaaaatcaa aaagaaaaat
     1321 tgaaaagcaa aaacatcgaa gtatcacaaa tgaccaagaa agaaataagt tttctagaac
     1381 aattgcaaaa taaaaatgaa gatatgcaaa aattcgaaaa gcaaatgaaa aaagaaggat
     1441 ttaaaaaaat aaagctagaa gacaaaaatt ctaaattaaa atatctagct acagaagcaa
     1501 atgataaaaa tgcatatggc tacatagcta ataacgctta taaaaatgaa aaaacaaaag
     1561 aaattgtagt ttctgaaaca atatatgata actacaacaa aaaaatcaca aaattcattg
     1621 ccgaaaaaag aagtatgact gaaaacaaag agggaaaaat acttattaac aaaaattacg
     1681 ttgaatataa ggctgaaaaa gttgataaaa acagaccggt aacatatgca ggattcaaat
     1741 ggaacggcaa agcattcgct tgtagtatgg ctggacttta tgcatgtgct cactactgtg
     1801 gagtatgggc aatcgtaaat ccaatagccg gcggtgtatg tgaagcagtt tgcggaactg
     1861 catttgcagc tggatgctct gtaggttgat tttattccaa ttg
//
LOCUS       DANRDI010000270         1898 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.275, whole genome shotgun sequence.
ACCESSION   DANRDI010000270 DANRDI010000000
VERSION     DANRDI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.275"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(136..624)
                     /locus_tag="PZ377_002809"
     CDS             complement(136..624)
                     /locus_tag="PZ377_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDM6359649.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(621..1841)
                     /locus_tag="PZ377_002810"
     CDS             complement(621..1841)
                     /locus_tag="PZ377_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDM6359650.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
BASE COUNT          746 a          336 c          261 g          555 t
ORIGIN      
        1 aaatatttcg aaaaaacatt ctcatacatt tttataaaca aaaagcttat tacatcatat
       61 gcgaatttgg tactgttaat gataaaatca ctaaattaca gttaccgaaa aacatatttt
      121 gcaataagct ttttattaat taatcaataa agcatctata aagaaccata caatcattat
      181 aaacataatg attgcttgag ctatactact tgttaaaaat gctacgattg aacctatact
      241 tactttaact gctctttcaa atgaatagcc ttgtattaat tcaactatga ataccaaaat
      301 aaaaggtata ataataattc caaatggcgg taaaacaaaa catccaataa ccacaccaat
      361 taaagctgca tactctccaa acttagaacc accgaagcga ttcacaaaat atttattagc
      421 taaaaagtcg gcacataaaa ttaatattgt tagcaatgcc atagatacat aaaacaccca
      481 tgataaatgc tgattatgaa agccaaattg atagattaaa aagccaaccc ataatactaa
      541 aacagaagga ataatcggct taatcaaccc aacaaatgct aacatgaagg cagcgatgat
      601 aagtagccat aaaataattg tcatgttgat atcacatcct cttttgtaat tttttgattt
      661 ttagtaaaga atataagtat catacctaac atgattgatg ctgctgatac aaaaaataca
      721 ttttccaaac ctacccagtg actcatagca ccacctaata ggttaccgcc taactgtcca
      781 ataaccattg cattagaaaa tagtgttgaa gcgtatcctg gaaaatctgg taagatatct
      841 tggaaataac taataccaat tcctaaaaga accgctaaga aaatagctaa aaacacctgt
      901 cctgctaaca tcatatagaa gtttttaaat accccaatgc tgaagtagaa taaaccacca
      961 aaaatcgctc cataaatcaa caatgttcga gtctgtaatc tagatgataa aacgccaaga
     1021 atgatcataa atggcacttc taaaccagca cataaactag ctaaataacc gacatgttgt
     1081 tcattttctt ttaaataatc agtaacaaat aaaggcatat tcatcgtata catccattgt
     1141 ccaatgtgta ataaaataaa tgcaataaat ggtaataaaa gcgttttgtc tttaaacata
     1201 ttaggagcaa ttttttcaac atgttgttgc gtactaatag ggtgtttaat gtttaaatcc
     1261 ttatagaaaa acacttgaag tactaaagta aataaaatga tacttattgt tccaccaaac
     1321 aatccagcat agccttttaa tccgattaat tgggcaccaa taaatggacc aaataaaaag
     1381 cccaatgaga acattgaacg taatactgtg ttggcaaatt gagctctatc tttagagctt
     1441 gaaacattga tagattctct agcagatgcg taaagttggg gcattgctgg tgcaaataat
     1501 ccttggaaaa tcgcatatag taatatgaat aaccagattg tatctacaaa aaagtatatt
     1561 gaaaaaccaa gcgcacccat taataatgcg agaataataa taatttttct attaaagtga
     1621 tgcgtatccg aaaatctagc aataattgaa tttactgtaa actggctaat cgctgcagat
     1681 gctagaagta atccatactg atttgttgtc atacctaaat ctttagttgc aaaaagaaca
     1741 agatatggga ccgtaaccgc aatacccata cctagtagaa acatattagc aacaaagagt
     1801 ttataattct ttatttgtaa taacgctgca aacatatcca taaccccgct tcttagagcc
     1861 ccttttaatt tattaattag gggctcttat gcagttgg
//
LOCUS       DANRDI010000271         1886 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.276, whole genome shotgun sequence.
ACCESSION   DANRDI010000271 DANRDI010000000
VERSION     DANRDI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.276"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            7..312
                     /locus_tag="PZ377_002811"
     CDS             7..312
                     /locus_tag="PZ377_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDM6359651.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            302..>1886
                     /locus_tag="PZ377_002812"
     CDS             302..>1886
                     /locus_tag="PZ377_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDM6359652.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKID"
BASE COUNT          736 a          260 c          367 g          523 t
ORIGIN      
        1 ctttatatga aattaacaaa aaaacagctg aaagaatata tagaggatta taaaaaatct
       61 gatgacatat taattaattt gtatatagaa acgtatgaat tttattgtcg gttaagagat
      121 gaacttaaaa atagtgattt gatgatagag catacaaaca aggctggtgc gagcaatatt
      181 gttaagaatc cattaagcat agaactgaca aaaacagttc aaacactaaa taacttactc
      241 aagtctatgg gtttaactgc agcacaaaga aaaaagatag ttcaagaaga aggtggattc
      301 ggtgactatt aaagttttaa atgaaccttc accaaaacta ttaacaacat ggtatgcaga
      361 gcaagtcact caagggaaaa taaaaacaag caaatatgtt aaaaaagaat gtgagagaca
      421 ccttagatat ctagaaaatg gaggtaaatg ggtatttgat gaagaattag cgcaccgtcc
      481 tattcgattc atagaaaagt tttgtaaacc ttccaaagga tctaaacgtc aacttgtatt
      541 acaaccatgg caacatttta ttattggcag tttgtttggt tgggttcata aagaaacaaa
      601 actgcgcagg tttaaagaag ctttgatatt tatggggcga aaaaatggta aaacaactac
      661 tatatctggt gttgctaact atgctgtttc tcaagatgga gaaaacggcg ctgaaatcca
      721 tcttttagca aacgtaatga aacaagctag aatattattc gatgaatcta aggcgatgat
      781 aaaagctagc ccaaagcttg ataaaaattt cagaacatta agagatgaaa tccattatga
      841 cgcaacgata tcaaaaatta tgccccaagc atcagatagc gataagttag atggattgaa
      901 tacacacatg gggatttttg atgaaattca tgaatttaaa gactataaat tgatttcagt
      961 tataaaaaac tcaagagctg caaggttaca acctcttctc atctacatta cgacagcagg
     1021 gtatcaatta gatggtccac ttgttgatat ggtagaagcg ggaagagaca ccttagatca
     1081 aatcatagaa gacgaaagaa ctttttatta tttagcatct ttggatgatg acgatgatat
     1141 taatgattcg tcgaactgga taaaagcaaa tcccaactta ggtgtctcta taaatttaga
     1201 tgagatgaaa gaagagtggg aaaaagctaa gagaacacca gctgaacgtg gagattttat
     1261 aaccaaaagg tttaatatct ttgctaataa tgacgagatg agttttattg attacccaac
     1321 actccaaaaa aataatgaaa ttgtttcttt agaagagctg gaaggcagac catgcacgat
     1381 tggttatgat ttatcagaaa cagaggactt tacagccgcg tgtgctactt ttgcgttaga
     1441 taatggtaaa gttgcagttt tatcgcattc atggattcct aagcacaaag ttgaatattc
     1501 taacgaaaaa ataccctata gagaatggga agaagatggc ttattaacag tgcaagataa
     1561 gccttatatt gactaccaag atgttttaaa ttggataatt aagatgaatg agcattatgt
     1621 agtagaaaaa attacttatg atagagcgaa cgcattcaaa ctaaatcaag agttaaaaaa
     1681 ttacgggttt gaaacggaag aaacaagaca aggagctttg accttgagcc ctgcattgaa
     1741 ggatttaaaa gaaatgtttt tagatgggaa aataatattt aataataatc ctttaatgaa
     1801 atggtatatc aataatgttc agttgaaact agacagaaac ggaaactggt tgccgtctaa
     1861 gcaaagcaga tatcgtaaaa tagatg
//
LOCUS       DANRDI010000272         1813 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.277, whole genome shotgun sequence.
ACCESSION   DANRDI010000272 DANRDI010000000
VERSION     DANRDI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.277"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..761)
                     /locus_tag="PZ377_002813"
     CDS             complement(<1..761)
                     /locus_tag="PZ377_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDM6359653.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADIL
                     EILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQK
                     VIEKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFM
                     RSTEVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVAN
                     VKAIKPNRKGDKDIESAIKETSKYSVKSSDFLTDD"
     gene            1213..1794
                     /locus_tag="PZ377_002814"
     CDS             1213..1794
                     /locus_tag="PZ377_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359654.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIA
                     DKNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDI
                     ENKKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVA
                     KKLAKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
BASE COUNT          664 a          294 c          227 g          628 t
ORIGIN      
        1 tcatcatcag ttaaaaaatc agatgattta accgaatatt ttgaggtctc tttgattgcc
       61 gattcaatgt ctttatcgcc ttttctattc ggtttgatcg ctttaacatt agcaacaggt
      121 cgatagtcaa cttgtaatgc tctttgccat aaattaaccc attcttcttg agttatataa
      181 ttctcttttt ttctaaaata tgcattttca acacataaca aaacatgcat gtgctgatta
      241 taactaccgt catttttatt aacggtaact tctgttgaac gcataaatcc aacaagattt
      301 tgtttaacct ttttatatct actcaaccta tcaaatgctt tagttagatg cttcaaactt
      361 tgttctaaag tatctccatc tatcgcattt tttgttgaaa gtgttaaaaa caaccaacgt
      421 gcttttggct tttccttaat tactttttca atcacttttt gagcttgata actatttttc
      481 atagcacgtc tccaattaca aaccggacat aattttgatt tacaaaacca tgtcttatgt
      541 aacttcaaat agccttcatc agttggctta aattctaaaa tattaccaca ttgttttaca
      601 ttaaaagcct ttttgatttt taaaatttct agtatatctg catagcttac attatctatt
      661 ttcttttctc tccatgggcg ttgtttccca cttttcgtca tatcttttaa cgttttatta
      721 tcttgatttt cggttatact tctagtagta ttatattgca tataaaaaga cccccagtat
      781 tatttaagat tcgacacctt aataatataa ctggggtttt ttattgtcaa attttccttt
      841 cacgcacaaa aacacgttta aacactgtaa tatcaacagt tacagctata tatataaaat
      901 cgagttattt ctatatagta tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa
      961 caacctattc cttcttcttt ttatgaccct ctaaacctaa ataaattaat gtgaaaacca
     1021 atgaataaat aaaactttct aaaaagtcaa aaggattatc aaataaaatc gaattaacaa
     1081 aattaaaaat taaaaataca gataataaaa taatcaaaaa gtaaaaaaac ttattcatat
     1141 taaactcctt tgtttttata tgttaatatt atattgaatt catttaaata acaaaacaag
     1201 ggggcattta caatgaatgc aattcaaaac agtatatcac taagaaagat tttagtagta
     1261 tctttaagta ttttaaccat actttcaatg gtcttggatt ttaatttcaa agaagctcaa
     1321 gcccaaaaca aaaataacat tgcagataaa aacgttgaaa ctttaaacga aaaagaaatt
     1381 gaaaaagaac taaaatacat atatggcaaa attattattt tagataaaga cggaacagct
     1441 aaagatgtta atctagaaaa tgccaaatct cgatatggat atgttcctga aggattccaa
     1501 aaactaaaaa acgacataga gaataaaaag aaaactagtt caatatcacc tagagcagtt
     1561 ggtgggaact acaaaaatag cggtgactgt ttctattctg aagttttaaa ctcatatgga
     1621 gaacttttaa ctggaaacat aatcacagca gtttttgaag atgttaaagc caaaaactta
     1681 aagggcgtag caaaaaaatt agctaaaatt gggataaaag gaaacctagc tggaatcgca
     1741 gctactatgg tttcgaagga tgtacaatgt aatcttaaat acgggttact ttagatacta
     1801 tttattccaa ttg
//
LOCUS       DANRDI010000273         1757 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.279, whole genome shotgun sequence.
ACCESSION   DANRDI010000273 DANRDI010000000
VERSION     DANRDI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.279"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1263
                     /gene="nrdD"
                     /locus_tag="PZ377_002815"
     CDS             <1..1263
                     /gene="nrdD"
                     /locus_tag="PZ377_002815"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDM6359655.1"
                     /translation="VTLGFGLGTDHLSRKIQQAILNTRIKGLGKDRTTAIFPKLVFSI
                     KKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDKLVEILGDFKAPMGCRSFLPSWK
                     DAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFWEIFYERIDVLHDALLYRINRLK
                     DAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRATISMGYIGLYETATVFYGPDWET
                     SQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTPSESLTDRFCRLDQERFGDIKDI
                     TDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASGGFIHYCEYPKLQHNLKALEAVW
                     DYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFKCPNCGNDNPKTVDVVKRTCGYL
                     GNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            1260..>1757
                     /gene="nrdG"
                     /locus_tag="PZ377_002816"
     CDS             1260..>1757
                     /gene="nrdG"
                     /locus_tag="PZ377_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDM6359656.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQS"
BASE COUNT          615 a          256 c          341 g          545 t
ORIGIN      
        1 gtaacattag gattcggctt aggtacagat catttaagtc gcaaaattca acaagctatc
       61 ttaaatactc gtatcaaagg cttaggaaaa gaccgcacga cagcgatttt cccgaaactt
      121 gtattttcaa ttaaaaaagg aaccaacttt agtccgcaag atccgaacta tgacattaaa
      181 caactagcat taaagtgttc aacgaaacgt atgtatccag atattttaaa ttatgacaaa
      241 ctcgtagaaa tattaggtga tttcaaagcg ccaatgggtt gtcgttcatt tttaccaagt
      301 tggaaagatg cggaaggtca ttttgaaaat aatggtcgtt gtaatcttgg tgttgttaca
      361 cttaatttac ctagaatggc attagaatct gccggtaata tgacgaaatt ctgggaaatc
      421 ttttatgaac gtatcgatgt gttacatgat gcattacttt atcgtataaa tcgtttgaaa
      481 gatgctgtac cgaataacgc accgatttta tataaaagtg gcgcatttaa ctataaatta
      541 aaagaaacag atgatgttgc tgagttattt aaaaataaac gtgcaacgat ttcaatgggc
      601 tatatagggt tgtatgaaac agctactgtt ttctatggtc cagactggga aacatctcaa
      661 gaagcaaaag catttacgct tgaaattctt aaagaaatga aacgttatca aacgaaatgg
      721 acagaattat atgacatttg gttcagtatt tacagtacgc cgagtgaatc gctaacggat
      781 cgtttttgtc gtttagacca agagagattt ggagatatta aagacattac agataaagga
      841 tattatcaaa actctttcca ttatgatgta cgtaaagatg ttacaccttt tgaaaagtta
      901 gattttgaaa aagattatcc ttattatgcg agtggtggtt tcattcacta ttgtgagtat
      961 ccgaaattgc aacacaattt gaaagcacta gaagcggtat gggactactc ttatgacaaa
     1021 gttggttact taggtacaaa tattccgatt gatcattgtt atgaatgtga ttacgatgga
     1081 gattttgaag caactgaaaa aggatttaaa tgcccgaact gtggcaatga taatcctaaa
     1141 acagttgatg tcgttaaacg aacatgtggt tacctaggca atccagttca acgtccagta
     1201 attaaaggcc gtcataaaga aatttgcgca cgagtaaaac atatgaaagc gcctaaagaa
     1261 tgatactttt agacattaaa caaggacaag gttatattgc taaaatagaa tcaaatagct
     1321 ttgttgacgg tgaaggagta agatgcagtg tttatgtatc aggatgtcca tttaattgtg
     1381 ttggatgtta taacaaagcc tcacaaaagt tcagatatgg cgagaaatac actgatgaaa
     1441 tattagcaga aatattagat gattgcgatc atgattatat atctgggcta agtctattag
     1501 gtggcgaacc attttgtaat ttggatatta cattaaatct tgtcaaagca tttcgagcac
     1561 gttttggaaa tacaaagaca atttgggtat ggactggatt tttatatgaa tatttagcaa
     1621 atgattgtac agaacgtcga gagttattat catacattga cgttttagta gatggtctat
     1681 ttatacaaca cttattcaaa cctgatttac catataaagg ttctttaaat caacgcatta
     1741 tagatgtaca acaatca
//
LOCUS       DANRDI010000274         1753 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.280, whole genome shotgun sequence.
ACCESSION   DANRDI010000274 DANRDI010000000
VERSION     DANRDI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.280"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            367..1542
                     /gene="rpsA"
                     /locus_tag="PZ377_002817"
     CDS             367..1542
                     /gene="rpsA"
                     /locus_tag="PZ377_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDM6359657.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
BASE COUNT          662 a          214 c          329 g          548 t
ORIGIN      
        1 actatatacg gtcttcttac tattcaaatg taaaagttgc ttatttgcgt ggctttttgt
       61 tttataaaag tataaaattt tactataata tatcttgtag agaacaatga aatgacagac
      121 ttataaaaca aaatagtaag ctatgctaat ttttatacaa aaaatatgta taaacatagc
      181 aaactgtatt actttgatac aaaaatggtt gtaataaata tttatcgata tgacgacttg
      241 aatatgataa agtgacatat ttatgtatat gactatttcg caaaatgtaa tcgaggtaga
      301 atttcttgac aattctgtca gtttataaga tgttataaat atgtagtgta taaggaggca
      361 aacaagatga ctgaagaatt caatgaatca atgattaacg atattaaaga aggtgacaaa
      421 gtcactggcg aggtacaaca agttgaagac aagcaagttg ttgttcatat caacggtggt
      481 aaatttaatg ggattattcc tattagtcaa ttatctacgc atcatattga tagcccaagt
      541 gaagttgtaa aagagggcga cgaagttgaa gcatatgtca ctaaagttga gtttgatgaa
      601 gaaaatgaaa ctggagctta catcttatct agaagacaac ttgaaactga gaagtcttat
      661 agttatttac aagaaaaatt agataataat gaaatcatcg aagcgaaagt aacagaagta
      721 gttaaaggtg gtttggttgt tgatgtagga caaagaggtt ttgttccggc ttcactaatt
      781 tcaacagact tcattgagga tttctctgtg tttgatggac aaacaattcg tattaaagtt
      841 gaagaattgg atcctgaaaa taatagagtc attttaagcc gtaaagcagt tgaacaagaa
      901 gaaaacgatg ctaaaaaaga tcaattatta caatctttaa atgaaggcga tgttattgat
      961 ggtaaagtag cgcgtttaac tcaatttggt gcatttatag acattggcgg tgttgatggt
     1021 ttagtgcatg tatctgaact ttctcacgaa catgttcaaa caccagaaga agtagtttca
     1081 attggtcaag atgttaaagt taaaattaaa tctattgata gagatacaga acgtatttca
     1141 ttatcaatca aagatacgtt accaacacct ttcgaaaata ttaaaggtca attccacgaa
     1201 aatgatgtca ttgaaggtgt cgtagtaaga ttggcaaact ttggtgcatt tgttgaaatt
     1261 gcaccaggtg tacaaggact tgtacatatt tctgaaattg cacacaaaca cattggtacg
     1321 ccaggtgaag tgttagaacc tggtcaacaa gtaaatgtta aaatattagg tattgatgaa
     1381 gagaatgaaa gagtatcact atctattaaa gcaacattac caaacgaaga tgttgttgaa
     1441 agtgatcctt ctacgactaa ggcgtactta gaaaacgaag aagaagataa tccaacaatt
     1501 ggcgatatga ttggtgataa acttaaaaat cttaaactat aatttaatat ttaatagtca
     1561 actccacatg tttatgattg catgtggagt atttttatgt aacaaaatat actcggaatg
     1621 ataacgtgga caaatttaac taagtgttta aaaagataaa gttttaagtg ctgattttta
     1681 tcattacagt aataaactca ttttgaatac acagtctcat gtgatattat taaaaagata
     1741 taagaaagag agg
//
LOCUS       DANRDI010000275         1744 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.281, whole genome shotgun sequence.
ACCESSION   DANRDI010000275 DANRDI010000000
VERSION     DANRDI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.281"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..600
                     /locus_tag="PZ377_002818"
     CDS             <1..600
                     /locus_tag="PZ377_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDM6359658.1"
                     /translation="DREYTEVKVNNNRFKGIIGLVTMKKVRDPLEVTVVGQNIIVADD
                     NYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGNARTVDLCLDVLALPS
                     GEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDFKGFQKKIMYCFNKIN
                     AKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            716..1582
                     /locus_tag="PZ377_002819"
     CDS             716..1582
                     /locus_tag="PZ377_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDM6359659.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
BASE COUNT          688 a          229 c          315 g          512 t
ORIGIN      
        1 gatagggaat acacagaggt aaaagttaat aataataggt ttaagggtat tataggctta
       61 gtcacgatga aaaaggttcg tgatccttta gaggtgacgg tagttggaca aaatatcatt
      121 gtcgcagatg acaattataa atggttgcaa atactacctg aaaagaaacg ttatagtata
      181 actgtaatgt ttgataataa aggcaatcca ttagaatatt attttgatat aaatatcaaa
      241 aatataacgc aaaaaggtaa tgcgcgtaca gtagatttat gtttagatgt tttagcatta
      301 ccaagtggtg aatatgagtt ggtagatgaa gatgatttaa tgtttgcatt agaaagtgag
      361 caaattacaa aaaagcaatt tcatgaagca tatatgattg cacatcaaat tatggcagag
      421 ttagaaaatg attttaaagg attccaaaag aaaatcatgt actgctttaa taaaattaat
      481 gcaaaggctc aaaaaaatca tcaaaagcca caaaataaaa ctaatattga aaaaagcaaa
      541 caaataaagc ctaagcaata taatcaaact aaaaatcacc aacaacaaaa gaaaaactaa
      601 gtaattcaag ctgcagccat accaataaaa ttggtgaaat tattcgttag taatgaactc
      661 atgaatgctt gtagccatga aagttcaata attgaataat ttatgggggg aaattatgaa
      721 gattgaagac tatcgtttac taataacatt agacgaaacg aaaacgttac gtaaagcggc
      781 tgaaatttta tatatatctc aacctgctgt tacacaaaga ctaaaagcta ttgaaaatgc
      841 ttttggagta gatattttta tcagaacaaa aaaacaattg attacaacaa ctgaaggaac
      901 aatgattatt gagcatgctc gtgacatgtt gaaaagagag cgattatttt ttgacaaaat
      961 gcaggcacat attggtgaag tgaatggaac aatatcaatc gggtgttctt ctttgattgg
     1021 acaaacctta cttcctgaag ttttgagcct atataatgcc caatttccta atgttgaaat
     1081 acaagtgcaa gttggttcaa ctgaacaaat taaagcaaat catagagatt atcatgttat
     1141 gataactcgt ggaaataagg taatgaattt agctaacaca catttattta atgatgatca
     1201 ttattttatt tttccaaaaa atagacgaga tgatgttaca aagttaccat ttatagagtt
     1261 tcaagctgat ccgatttata taaatcaaat aaaagaatgg tataacgata atttagaaca
     1321 agattaccat gcaactatta cagtggatca agtagcaact tgcaaagaaa tgttgattag
     1381 tggtgtaggt gttacaatct tgccagaaat tatgatgaaa aatatcagca aagaacaatt
     1441 tgagtttgaa aaagtagaaa ttgataatga accgctgatt cgttcgacat ttatgagtta
     1501 tgatccgagc atgttgcaat tgccacaagt tgattctttt gtaaatctca tggcgagctt
     1561 tgttgaacaa ccaaaggcgt agttttagac taatttaagg tttgtattta attttaaact
     1621 attcggttaa attgaacgta gttggttgct aatgcaccaa cagcaaaaga gcccctaatt
     1681 aataaattaa aaggggacaa aggaatacag ttggttgcta atgcaccaac tgcataagag
     1741 cccc
//
LOCUS       DANRDI010000276         1740 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.282, whole genome shotgun sequence.
ACCESSION   DANRDI010000276 DANRDI010000000
VERSION     DANRDI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.282"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(636..1082)
                     /gene="perR"
                     /locus_tag="PZ377_002820"
     CDS             complement(636..1082)
                     /gene="perR"
                     /locus_tag="PZ377_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDM6359660.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(1179..>1740)
                     /locus_tag="PZ377_002821"
     CDS             complement(1179..>1740)
                     /locus_tag="PZ377_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDM6359661.1"
                     /translation="TGKRLSGQTVLFLGTGAIATRTAKLAKAFNMNLIGLSKSGQNKD
                     EFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMKDEALFINIGRGSIV
                     KEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAHITGNDYEAKYDLLD
                     IFKNNLVNFLNKNGLIENEVDAKKGY"
BASE COUNT          560 a          263 c          204 g          713 t
ORIGIN      
        1 tttgtcactt acaagattta attataactc gtaagttttt gtaagtcaag aattattttt
       61 aattatttta tttctaaaaa ataaatttcc taactcaatt aattcaacaa atattatttt
      121 attatgtatt tgttcgaatt tcaatagtca aatttacact ttattaatat tttaaatcaa
      181 caacttaaaa catttgtttt ttaaatatta gtgccgtttg acgactttta tataatatca
      241 agttattcta gtagagtcaa cataaaacaa cacttatttt tgtttaaaaa ttactgttga
      301 agggattatt ttctattcgt aagtactata tgtatttaaa gtgtaattcc ttatgctttt
      361 tacttcagtt ttacaaacaa acttatactc actttatgga tagttattta gtagaccttc
      421 gtttgaatat ttttatgttt atttataaag gatactagtt attagttaat accacttccg
      481 tagttagaat attcttttct tcaatttaaa tatttttcag aataagcata aaaaaaagtt
      541 aaacaacttt catgtcgttt aacttcttaa atattacgct tacttatatg aagtaagcta
      601 acgctttttt aatttgtcat actaccaaag ttaaattatt tatcttggca ttctttacaa
      661 actccataaa tttccattcg atgatgtgtt acgtcaaagt cagtcatatg ctgagctaat
      721 ctttcaattt catttaactg tggatattga aaatcaacaa tcttaccaca ttgttcacat
      781 ataatatgat aatgattatg tgtattaaag tcgaatcgac ttgatgagtc tccatatgtt
      841 aattctttta caattccaat atctttaaac actcttaagt tattatatat tgtcgcaaca
      901 cttatatttg gaaaatcagg tgaaagtgct tgataaattt catcagctgt tggatgagta
      961 tgtgaagaaa ttaaataacg taatattgct tgtctttgag gtgtaattct tacgcctgct
     1021 tgtcgcaatg atgcaattga ttcttctagt tcatgttcaa ttgattctat ttcaacactc
     1081 atctatatca ccatctttct tatataataa ttattattac ttactataaa tataactgta
     1141 gtattgcgcg cgtgtcaata tttacatgat gatttcattt aatagccttt tttagcatca
     1201 acttcattct caattagacc attcttattg agaaaattaa ctagattgtt tttaaaaata
     1261 tctaataagt catactttgc ttcataatca ttaccagtta tatgcgctgt tatagttaca
     1321 ttatccaatt catataattc atgattaggt ttcaaaggtt cattttcaaa cacatctaaa
     1381 tatgcatgtc gaataacttt actttttaat acttctatta agagcgcttc tttaactatg
     1441 ctacctcgtc ctatatttat aaaaagtgct tcatctttca ttaattcaaa atgttttttc
     1501 tttagtaaat gaatcgtttc ttgcgtttct ggtaaagcat ttataataat gtcagcattt
     1561 ggtaatgtgc tttcaagtga ttcgatagta tatatctcgt caaactcatc tttgttctga
     1621 cctgacttgc tcagaccaat taaattcata ttaaaagcct ttgctaactt cgcagttcta
     1681 gtagcaattg cacctgtacc taaaaataaa actgtttgtc ctgataggcg tttaccagtt
//
LOCUS       DANRDI010000277         1683 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.283, whole genome shotgun sequence.
ACCESSION   DANRDI010000277 DANRDI010000000
VERSION     DANRDI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.283"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1494
                     /locus_tag="PZ377_002822"
     CDS             <1..1494
                     /locus_tag="PZ377_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDM6359662.1"
                     /translation="YVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLTIA
                     LIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGAIV
                     YTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHMYD
                     AFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFDIP
                     KDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAITA
                     SANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQGE
                     FKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIGDV
                     GNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEEKV
                     REIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
BASE COUNT          595 a          225 c          335 g          528 t
ORIGIN      
        1 tacgtacttg aaaataaaga taaattgaag ggtatattcc ttacacacgg acatgagcac
       61 gcgattggtg cagtgagtta tgttttagaa caattagatg caccagtata tggatctaaa
      121 ttgacaatag cgttaattaa agaaaatatg aaagcccgta atattgataa aaaagttcgc
      181 tactatacag ttaataatga ttcaattatg agattcaaaa acgtgaatat tagtttcttt
      241 aatacgacac acagtattcc tgatagttta ggtgtttgta ttcacacttc atatggtgcc
      301 attgtgtata caggtgaatt taagtttgac caaagtttac atggacatta tgcaccagat
      361 attaaacgta tggcagagat tggtgaagaa ggcgtatttg tcttaatcag tgattctact
      421 gaggcagaga aacctggata taatactccg gaaaatgtga ttgaacatca tatgtatgat
      481 gcttttgcaa aagtgcgagg tcgcttgata gtttcatgtt atgcttcgaa ctttatacgt
      541 attcagcaag ttttaaatat tgctagcaag ctaaatcgta aagtgtcatt tttaggaaga
      601 tcacttgaaa gttcatttaa tattgctcgt aaaatggggt atttcgacat tcctaaagat
      661 ttgctaattc ctataacaga agttgataat tatcctaaaa atgaagtgat aattatagct
      721 actggtatgc aaggagaacc tgtagaagcc ttaagtcaaa tggcgcaaca taagcataaa
      781 attatgaata tcgaagaagg cgattctgta tttttagcaa ttacggcttc tgctaatatg
      841 gaagttatca ttgcgaatac attaaatgag cttgtacgtg ctggcgcaca tattattcca
      901 aataacaaaa agattcatgc ttcaagtcat ggttgcatgg aagaattaaa aatgatgatt
      961 aatattatga aacctgaata ctttattcct gtacaaggtg aatttaaaat gcagatagca
     1021 catgcgaagc tagcagctga agcaggtgtt gcaccagaaa agattttcct tgtggaaaaa
     1081 ggagatgtca ttaattacaa cggtaaagat atgatattaa atgaaaaggt aaattcagga
     1141 aatattttaa tagatggcat tggtattggg gatgtaggaa atatcgtgtt gagagaccgt
     1201 catcttttag cagaagatgg tatctttatt gctgttgtaa cgttagatcc taaaaataga
     1261 cgtatagctg cgggacctga aattcaatct cgtgggtttg tatatgtacg tgaaagtgaa
     1321 gacttattac gtgaagcaga agagaaagta cgtgaaatag tagaggctgg tttacaagaa
     1381 aaacgcatag aatggtctga aattaaacaa aatatgcgtg atcaaattag taaactatta
     1441 ttcgaaagta caaaacgtcg tcctatgatt attccagtaa tttctgaaat ttaatcaaaa
     1501 agtcattaac ataaaagagg tcagaacaag tcactgaaat ataatggttg tcatggacaa
     1561 tttacttata ttttatgatg gtcaattgaa ggggtaacga ttaatctgtt atcttaagta
     1621 aattgataca tagatgatat tgttctaacc tctttcatcg tctgtttgga ctacatattc
     1681 taa
//
LOCUS       DANRDI010000278         1601 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.284, whole genome shotgun sequence.
ACCESSION   DANRDI010000278 DANRDI010000000
VERSION     DANRDI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.284"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..244
                     /locus_tag="PZ377_002823"
     CDS             <1..244
                     /locus_tag="PZ377_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="predicted polysaccharide polymerase involved in
                     biofilm formation; required for the synthesis of the
                     beta-1,6-N-acetylglucosamine polysaccharide; PgaC; in
                     Yersinia the HmsR protein is an inner membrane protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDM6359663.1"
                     /translation="FLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVY
                     WIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR"
     gene            208..513
                     /gene="icaD"
                     /locus_tag="PZ377_002824"
     CDS             208..513
                     /gene="icaD"
                     /locus_tag="PZ377_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDM6359664.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            510..1382
                     /gene="icaB"
                     /locus_tag="PZ377_002825"
     CDS             510..1382
                     /gene="icaB"
                     /locus_tag="PZ377_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDM6359665.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            1369..>1601
                     /locus_tag="PZ377_002826"
     CDS             1369..>1601
                     /locus_tag="PZ377_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine export
                     protein"
                     /protein_id="HDM6359666.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLT"
BASE COUNT          594 a          229 c          256 g          522 t
ORIGIN      
        1 atttctacta tcatcattta ctatgacttt tataaacgtt attcaattta cagtcgcact
       61 ctttattgat agtcgctacg agaaaaagaa tatggctgga ctcatatttg taagttggta
      121 tccgacagta tactggatta ttaacgcagc agtagttctt gtcgcatttc caaaagcatt
      181 aaaacgtaag aaaggtggtt acgcaacatg gtcaagccca gacagaggga atacccaacg
      241 ctaaaatcat cgctaaatat tgtaagagaa acagcactta tcgctatatc ttgtgtcttt
      301 tggatatatt gtttagttgt tctactcgtt tatattggta ctatatttga aattcatgac
      361 gaaagtatca atacaatacg tgttgcttta aacattgaaa atactgaaat tttagatata
      421 tttgaaacta tgggcatttt cgcgattatc atttttgtat tttttacaat tagcatattg
      481 attcaaaaat ggcagagagg aagagaatcg tgaagtatag aaaatttata attttagtgt
      541 tgagtatctt gatcatattg cctgtaagca cactggatgg tcatcatatt gcaaatgcag
      601 atgacgattc acctaaaaaa ctgaaatata aagaaaatag tgctctggca ttaaattatc
      661 accgtgtaag aaaagcgaat tttctgaata attttattta cttcttttct agtagtaaag
      721 aaattaaaaa ttatagtgtt agtcaatcac aatttgaatc tcaaataaaa tggctaaaat
      781 cacatgatgc taaattttta accttgaaag aatttttata ttacaagaaa aaaggtaagt
      841 ttccaaaacg aagtgtatgg attaactttg atgatatgga tgaaactatt tatgaaaatg
      901 cttatccaat cttaaaaaaa tataaaatac cggcaactgg gtttattatc acaggtcatg
      961 ttggggaaga aaactttcac aacctcgata tgattagtaa aaaagaacta aaagaaatgt
     1021 ataaaactgg gttatgggaa tttgaaacac atacccacga tttgcataac ttatctaaaa
     1081 ataataagtc aaaattaatg aaagcttctg aagctacaat cataaaagat ttaaacaaaa
     1141 gtgaaaaata tctaactaaa aactttaaaa agtcgcagaa aactatagcc tatccttatg
     1201 gcttgatgaa tgacgataaa ttaccggtaa tcaaaaaagc tgggttaaaa tacggttttt
     1261 cattagagga aaaagcagtc actccgaact ccaatgatta ttacatccct agaatattaa
     1321 ttagtgatga tgcttttgag catttaatta agagatggga cggattccat gaaaaagatt
     1381 agacttgaac tcgtatattt acgtgctatt atatgtgcaa ttattattat cacacattta
     1441 cttacacaaa ttactttaaa acatgaaaat atggagggtg gatccttagt gttacaattt
     1501 tacattcgta atattgtgat ttttggtaca ccttgcttta ttatcttgtc acagttactg
     1561 acaaccttga attaccaaaa agtcacctat agatacttaa c
//
LOCUS       DANRDI010000279         1586 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.285, whole genome shotgun sequence.
ACCESSION   DANRDI010000279 DANRDI010000000
VERSION     DANRDI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.285"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..72
                     /locus_tag="PZ377_002827"
     CDS             <1..72
                     /locus_tag="PZ377_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359667.1"
                     /translation="EELEKYIKQHGLEYEEQKQLTLF"
     gene            86..292
                     /locus_tag="PZ377_002828"
     CDS             86..292
                     /locus_tag="PZ377_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359668.1"
                     /translation="MKIKIEKEVNLPELIQWAWDNPKLSGNKRFYSNDVERNCFVTFH
                     VDSILCNVTGYVSINDKFTVQEEI"
     gene            295..696
                     /locus_tag="PZ377_002829"
     CDS             295..696
                     /locus_tag="PZ377_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359669.1"
                     /translation="MKIKVKKEMRLDELIKWARENPELSKGKIFLAKSFSNGFVRFQR
                     NTNTCSISSFIPIDTPFIVEVEEEITEDTVFDRLFEVYELQEGACMSALHTSISINER
                     LENTFFPTKAFYILNDGLTMTLIWKDGRLVE"
     gene            693..1040
                     /locus_tag="PZ377_002830"
     CDS             693..1040
                     /locus_tag="PZ377_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359670.1"
                     /translation="MMLKFKAWDKDKKVMSIIDEIDFNSGYILISTGYKSFNEVKLLQ
                     YTGFKDVHGVEIYEGDIVQDCYSREVSFIEFKEGAFYITFSNVTELLSENDDIIEIVG
                     NIFENEMLLEVMR"
     gene            1037..1345
                     /locus_tag="PZ377_002831"
     CDS             1037..1345
                     /locus_tag="PZ377_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359671.1"
                     /translation="MTFTLSDEQYKNLCTNSNKLLDKLHKALKDREEYKKQRYELIGV
                     IAKLRDCNKELEKKASAWDRYCKSVERDLINKFGNDDERVKFGMELNNKIFMEDDTNE
                     "
     gene            1338..1586
                     /locus_tag="PZ377_002832"
     CDS             1338..1586
                     /locus_tag="PZ377_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDM6359672.1"
                     /translation="MNNREKIEQSVISASAYNGNDTEGLLKEVEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIELDEAVGIMTGQVVYKYEEAQEND"
BASE COUNT          602 a          182 c          344 g          458 t
ORIGIN      
        1 gaagagcttg aaaaatatat aaagcaacat ggtttggaat atgaggaaca gaagcaacta
       61 actttatttt aaggagttgg aaatgatgaa aatcaaaatt gaaaaagaag tgaatttacc
      121 tgaacttatc caatgggctt gggataaccc caagttatca ggtaataaaa gattctattc
      181 aaatgatgtt gagcgcaact gttttgtgac ttttcatgtt gatagcatct tatgtaatgt
      241 gactggatat gtatcaatta acgataaatt tactgttcaa gaggagatat aacaatgaaa
      301 atcaaagtta aaaaagaaat gagattagat gaattaatta aatgggcgcg agaaaatccg
      361 gagctatcaa aaggaaaaat ttttcttgca aaaagtttta gtaatggatt cgttcgtttt
      421 caacgaaata caaatacgtg ttcgatatca agttttattc caattgatac tcctttcata
      481 gttgaagttg aagaggaaat cacagaagat acagtatttg ataggttgtt tgaagtgtac
      541 gagcttcaag agggagcctg tatgtcagcg ttacacacaa gtattagtat caacgaacgt
      601 ttagagaaca cgtttttccc taccaaagca ttctacatct tgaacgacgg cctaactatg
      661 acattaattt ggaaagatgg gagattggta gaatgatgtt gaaatttaaa gcttgggata
      721 aagataaaaa agttatgagt attattgacg aaatcgattt taatagtggg tacattttga
      781 tttcaacagg ttataaaagt ttcaatgaag taaaactatt acaatacaca ggatttaaag
      841 atgtgcacgg tgtggagatt tatgaagggg atattgttca agattgttat tcgagagaag
      901 taagttttat cgagtttaaa gaaggagcct tttatataac ttttagcaat gtaactgaat
      961 tactaagtga aaatgacgat attattgaaa ttgttggaaa tatttttgaa aatgagatgc
     1021 tattggaggt tatgagatga cgttcacctt atcagatgaa caatataaaa atctttgtac
     1081 taactctaac aagttattag ataaacttca caaagcatta aaagatcgtg aagagtacaa
     1141 gaagcaacga tatgagctta ttggggttat agcgaagtta cgagattgta acaaagaact
     1201 ggagaagaaa gcaagcgcat gggataggta ttgcaagagc gttgaaagag atttaataaa
     1261 caaattcggt aacgatgatg aaagagttaa attcggaatg gaattaaaca ataaaatttt
     1321 tatggaggat gacacaaatg aataatcgcg aaaaaatcga acagtccgtt attagtgcta
     1381 gtgcgtataa cggtaatgac acagagggat tactaaaaga ggttgaagac gtgtataaga
     1441 aagcgcaagc gtttgatgaa atacttgagg gtttacctaa tgctatgcaa gatgcactca
     1501 aagaagatat tgaacttgat gaagcagtag ggattatgac gggtcaagtt gtctataaat
     1561 atgaggaggc acaggaaaat gactaa
//
LOCUS       DANRDI010000280         1549 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.286, whole genome shotgun sequence.
ACCESSION   DANRDI010000280 DANRDI010000000
VERSION     DANRDI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.286"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            145..756
                     /locus_tag="PZ377_002833"
     CDS             145..756
                     /locus_tag="PZ377_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDM6359673.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
     gene            892..1110
                     /locus_tag="PZ377_002834"
     CDS             892..1110
                     /locus_tag="PZ377_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359674.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            1242..>1549
                     /locus_tag="PZ377_002835"
     CDS             1242..>1549
                     /locus_tag="PZ377_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDM6359675.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSVYELYKQFQNLRNLFKEEGHEPWTSCEF
                     "
BASE COUNT          568 a          149 c          281 g          551 t
ORIGIN      
        1 aattttataa attctgtgaa gtatacttat atataagata atcaacttca acgtatatca
       61 ccatattatt ttatactcaa tgatgtgaat ggatttaagt ggattgatat taaagtaaaa
      121 gttatggaat attgaggtgt gaatatggag ttcttattat taattgtcgt agccggactg
      181 tattatatta tatatttaac tgctgtgatg tattctgaaa aaatagtagt attgcctata
      241 atcatctatg ccattgtgtt tgtaataatt ggtatcactt atatctttat aggcgacagc
      301 tatgatcaat taacaaattt caatgtgatt ttgtatatgg ggagtttgtt ttatgcatgg
      361 atggctatta gaaatctttg gaacagacca ctattactaa aatataagaa cattacagat
      421 agttcaagtg gaatagttaa taaatctgaa tacaattcag ttgaaagctt acgtataaat
      481 attgaaatag ctaagtataa agggattatt tctttgatag tagctatagt actaacggta
      541 ttaatgacat taaagtcaac acctcaaatc actgcagaaa cacgtgactt aagtatctca
      601 tttttcatac tcagcttatt tatcattatt atatttgcag tttgggattt gtttattaga
      661 gttagaaaag gagcgtttgc ttttgttgta ataaggccaa tattatttag ttgttggtta
      721 tttattttga atatgatttt atcaagatta ttataattgt attacatata ggtgtgggta
      781 aatttttatt tatgaaatga taaaaaagta tatactatta atatcttttt tactataatt
      841 ataaatagtt aaataaattg aaagacaaaa taatgagtag gagttaatga gatggaaaac
      901 caaaaacaag gcaatggctt aaaaattgca acatgggtat ttattgtatt aacagtagtt
      961 acaccgctat ttggtattgg aagtattgtt tgtagtatta attataaaaa atacgatgct
     1021 gaaaaaggtt cgaagttatt gcaaattgca attatcgtaa caataattgc ttttgtttta
     1081 aatttattag catatttagg tttaagataa gtaaaacgaa tttgaagaag cattaagcga
     1141 catttgggtg ttgtttaatg ctttttattt aacggaaata agtgtagaga ataaattaat
     1201 agtttaatca agagatattt cgaacacata ggggcgataa catgactttc gaagaaaaat
     1261 taagtgaaat gtatagcgag attgcgaata agattagcag catgataccg gtagagtggg
     1321 aaaaggtata tgcaatggca tatatagatg acggaggagg agaagtgttc tactattaca
     1381 cagaacctgg aagtaatgaa ttatactact atactagtgt attaaataaa tatgatatat
     1441 cggaatcgga atttatggat tcagtgtatg agttgtataa acaatttcaa aatttaagga
     1501 atttatttaa agaagaagga catgaaccat ggacatcatg cgaatttga
//
LOCUS       DANRDI010000281         1536 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.287, whole genome shotgun sequence.
ACCESSION   DANRDI010000281 DANRDI010000000
VERSION     DANRDI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.287"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            425..1435
                     /gene="sspA"
                     /locus_tag="PZ377_002836"
     CDS             425..1435
                     /gene="sspA"
                     /locus_tag="PZ377_002836"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDM6359676.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
BASE COUNT          569 a          282 c          214 g          471 t
ORIGIN      
        1 ccgagttata ttcctctcct ttaaacacct cactcaaaat gattctatca aaaggtttta
       61 atatgctaac ttataattgt tcttcgaaac ttaagcactc aaataatata tcaccattca
      121 tattaatcta cctttggcca aacaattatt attcaaaagt tactatttat tattataaac
      181 aaatactttc cattcgctct caattccttt cttatttatt cttttagata aatgcaaaca
      241 attgacgatc ttatcaattt aactcaattg aataaagtat aatacaattc aattttaatt
      301 cccttaatgt ttttgtaaac tctaatcttt acaaaaactt tttaatttat ttatatattc
      361 ttattgactt aaaaattttt agtgttatat ttaacttgta aataaatttt ttggaggttt
      421 ttagatgaaa ggtaaatttt taaaagttag ttctttattc gttgcaactt tgacaacagc
      481 gacacttgtg agttctccag cagcaaacgc gttatcttca aaggctatgg acaatcatcc
      541 acaacaaacg cagtcaagca aacagcaaac acctaagatt caaaaaggcg gtaaccttaa
      601 accattagaa caacgtgaac acgcaaatgt tatattacca aataacgatc gtcaccaaat
      661 cacagataca acgaatggtc attatgcacc cgtaacttat attcaagttg aagcacctac
      721 tggtacattt attgcttccg gtgtagttgt aggtaaagat actcttttaa caaataaaca
      781 cgtcgtagat gctacgcacg gtgatcctca tgctttaaaa gcattccctt ctgcaattaa
      841 ccaagacaat tatccaaatg gtggtttcac tgctgaacaa atcactaaat attcaggcga
      901 aggtgattta gcaatagtta aattctcccc taatgagcaa aacaaacata ttggtgaagt
      961 agttaaacca gcaacaatga gtaataatgc tgaaacacaa gttaaccaaa atattactgt
     1021 aacaggatat cctggtgata aacctgtagc aacaatgtgg gaaagtaaag gaaaaatcac
     1081 ttacctcaaa ggcgaagcta tgcaatatga tttaagtaca actggtggta attcaggttc
     1141 acctgtattt aatgaaaaaa atgaagtgat cggaattcat tggggcggtg taccaaatga
     1201 atttaatggt gcggtattta ttaatgaaaa tgtacgcaac ttcttaaaac aaaatattga
     1261 agatatccat tttgccaacg atgaccaacc taataaccca gataatcctg ataaccctaa
     1321 caatcctgat aaccctaaca acccagatga accaaataac cctgacaacc ctaacaaccc
     1381 tgataatcca gacaatggcg ataacaataa ttcagacaat ccagatgcag cttaattaac
     1441 aattaagttc gtctagataa aatagaagca aagtcttagc aacgtaaaat attttgattt
     1501 aaatggaggg tattatatga atagttcatg taaatc
//
LOCUS       DANRDI010000282         1523 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.288, whole genome shotgun sequence.
ACCESSION   DANRDI010000282 DANRDI010000000
VERSION     DANRDI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.288"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..801)
                     /gene="gtfB"
                     /locus_tag="PZ377_002837"
     CDS             complement(<1..801)
                     /gene="gtfB"
                     /locus_tag="PZ377_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDM6359677.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQS"
     gene            complement(794..>1523)
                     /locus_tag="PZ377_002838"
     CDS             complement(794..>1523)
                     /locus_tag="PZ377_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDM6359678.1"
                     /translation="YEYQFSKAKYIDFFITATDIQNHMVCRQFEQYQGYRPRVYTIPV
                     GSIDALSYPTLSRKPYAMISASRLANEKHIDWLVKAVIVAKRQVPELTFDIYGEGSEK
                     TRLRKIIDTHRAQDYIRLLGHVKLDEIYNDYELFLSASTSEGFGLTLMEAVGSGLGMI
                     GLNVNYGNPTFIRDGENGYLVPFDTDEDSVDDVIAKLAHAIVMYFNNGPQTPHDISYE
                     VAQQFMTQDIILKWETLVQEVLHD"
BASE COUNT          487 a          295 c          233 g          508 t
ORIGIN      
        1 agattgaacc tttgcgtctg ctatattgag catcgtttca tattcgtttt tatctggaat
       61 aataatatta caatgtcgtt tcatatcacc tttacacatc aattgcatat ttcccgggac
      121 attaccatta cagtgttctt gccataccaa aacatcacta ccttttgatg gcaaattata
      181 taacactgaa aatggtaggg ctagtgagtt aataacgaaa tgatgttccg taatttcaag
      241 ttgcttgata aaaaataatg cgaatgcgag ctttgaaggg aaaaagtaag acttcccttg
      301 ccaatccaat atgacatcag atgttacaaa attttcataa atcacttctt taccttctgc
      361 tgtcatatat ttcttcaaga tcgctttacg atttaaatcg taaacagttt gtgcaaattt
      421 aataccattc ttagaataat aatcgacaaa tcggacacgt tgttggtcat caaaccattc
      481 gacacgacta acaattctag ggcgctctcc actttgataa aatattttac ctcgtagacg
      541 tcccatatca ttaattgtag ccgaattgtt gttaccttta atttcccaaa aagctggtac
      601 agtaacctga ttaaaaaatc gtggtttcat attttctgta ttatgattat ctgcaaaaaa
      661 ttgatacggt gatataacat cgtccggtaa aaagccattg tcattgagta caattgttaa
      721 atcttcttcc aacttactgg ctttaaaaga ctcatataac tttcgtgaat gatcgttaaa
      781 gtaatcaaat aatttaatca tgtagcacct cttgaactaa tgtttcccat tttaaaataa
      841 tatcttgagt cataaattgc tgtgccactt cataagagat gtcatgtggt gtctggggac
      901 cattgttaaa atacattaca atggcatgag ctagttttgc gataacatca tccacactat
      961 cttcgtcggt atcaaaaggt accaagtagc cattttcccc atctcgaata aaggttgggt
     1021 taccataatt cacatttaat ccaatcatac ctagtcctga gcctaccgct tccattagtg
     1081 ttaacccaaa accttcgcta gttgatgcag aaagaaataa ctcataatca ttataaattt
     1141 catcaagttt aacatgccct agtaaacgaa tataatcttg tgcgcggtgt gtatcaataa
     1201 ttttacgcag tcgcgtcttc tcgctacctt ctccataaat atcaaatgtt aattctggca
     1261 cttgtcgttt agccacgata accgctttga caagccaatc aatatgtttc tcattcgcta
     1321 aacgagatgc actaatcatc gcatatggct ttcttgataa tgttggatat gataatgcat
     1381 caatgcttcc cacaggaata gtataaacac gtgggcgata accttgatat tgctcaaatt
     1441 gtcgacaaac catatgattt tgaatatctg ttgctgtaat aaagaaatca atgtatttag
     1501 cttttgaaaa ttgatattca taa
//
LOCUS       DANRDI010000283         1479 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.289, whole genome shotgun sequence.
ACCESSION   DANRDI010000283 DANRDI010000000
VERSION     DANRDI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.289"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(145..249)
                     /locus_tag="PZ377_002839"
     CDS             complement(145..249)
                     /locus_tag="PZ377_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359679.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            344..>1479
                     /gene="sbi"
                     /locus_tag="PZ377_002840"
     CDS             344..>1479
                     /gene="sbi"
                     /locus_tag="PZ377_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HDM6359680.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNG"
BASE COUNT          622 a          258 c          215 g          384 t
ORIGIN      
        1 gaaccctatg catgagttgc gaagctatct aatgtcgtga actaattata tagaagaaaa
       61 agtgcatcaa tgacaaatta aatgagattt ctactctacc aaactctctt cgaaagacaa
      121 ttttctcctc tatttattag caaactattg catttctcca tatagtactt ccttacttaa
      181 aatacgctga atgttctgaa ttaaacgctt ttttacatag ttaacactag ttaatctatt
      241 agttaacatt agttaataat tagttaattt ccatttgtat tctcatgtga taaattctaa
      301 aagcatacaa taaatttaat atgtaaaaag aaagggaata cacatgaaaa ataaatatat
      361 ctcgaagttg ctagttgggg cagcaacaat tacgttagct acaatgattt caaatgggga
      421 agcaaaagcg agtgaaaaca cgcaacaaac ttcaactaag caccaaacaa ctcaaaacaa
      481 ctacgtaaca gatcaacaaa aagcttttta tcaagtatta catctaaaag gtatcacaga
      541 agaacaacgt aaccaataca tcaaaacatt acgcgaacac ccagaacgtg cacaagaagt
      601 attctctgaa tcacttaaag acagcaagaa cccagaccga cgtgttgcac aacaaaacgc
      661 tttttacaat gttcttaaaa atgataactt aactgaacaa gaaaaaaata attacattgc
      721 acaaattaaa gaaaaccctg atagaagcca acaagtttgg gtagaatcag tacaatcttc
      781 taaagctaaa gaacgtcaaa atattgaaaa tgcggataaa gcaattaaag atttccaaga
      841 taacaaagca ccacacgata aatcagcagc atatgaagct aactcaaaat tacctaaaga
      901 tttacgtgat aaaaacaacc gctttgtaga aaaagtttca attgaaaaag caatcgttcg
      961 tcatgatgag cgtgtgaaat cagcaaatga tgcaatctca aaattaaatg aaaaagattc
     1021 aattgaaaac agacgtttag cacaacgtga agttaacaaa gcacctatgg atgtaaaaga
     1081 gcatttacag aaacaattag acgcattagt tgctcaaaaa gatgctgaaa agaaagtggc
     1141 gccaaaagtt gaggctcctc aaattcaatc accacaaatt gaaaaaccta aagtagaatc
     1201 accaaaagtt gaagtccctc aaattcaatc accaaaagtt gaggttcctc aatctaaatt
     1261 attaggttac taccaatcat taaaagattc atttaactat ggttacaagt atttaacaga
     1321 tacttataaa agctataaag aaaaatatga tacagcaaag tactactata atacgtacta
     1381 taaatacaaa ggtgcgattg atcaaacagt attaacagta ctaggtagtg gttctaaatc
     1441 ttacatccaa ccattgaaag ttgatgataa aaacggcta
//
LOCUS       DANRDI010000284         1464 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.290, whole genome shotgun sequence.
ACCESSION   DANRDI010000284 DANRDI010000000
VERSION     DANRDI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.290"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(195..524)
                     /locus_tag="PZ377_002841"
     CDS             complement(195..524)
                     /locus_tag="PZ377_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359681.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            573..1310
                     /gene="sdpB"
                     /locus_tag="PZ377_002842"
     CDS             573..1310
                     /gene="sdpB"
                     /locus_tag="PZ377_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDM6359682.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
BASE COUNT          503 a          174 c          227 g          560 t
ORIGIN      
        1 atacattatg tactcatcta tttaaatctt agtaaatgta gttatataag actaaatcta
       61 ccatcttaat cattatgatt ataactcgat taatgcatct ttacaatttg aagaaacttt
      121 ttaattgtat aatttatgta ataaagtgaa ttttaataaa taacttgttt aaatgacatt
      181 ttatgaaaaa taggttaatg ggcaaattta attgcaaata atgcgttaga tagttctgta
      241 tgataaaaat taagatattg aattttgtta ccatagtcga cttgtcggag gggacgttta
      301 ttaagtagtg ttataatttc ataataggaa acttgatttt cgtttattat atatgatgtt
      361 tcaaagcctt tgaacttatt caattcatta gtaactattt ggttaaattt ataaatatgc
      421 gtttgaatgt tccaatctgt cattaaagta ttatatttaa tcgtatcaat gatttgaatc
      481 aaattagtaa tttgttgtct catatactta gtttcgttta tcatgtagaa gtacctccaa
      541 aaatcaatta ttaaacagga aaggatataa cagtggaaaa tgaaaaaaag aaatacacgt
      601 ttaaagatat agcatggaga gatttgctta taattccaat tataggtgtt atattttatg
      661 cactcacaat tgcagcttac ataggaccaa ttttgtttgg ttattatgaa atcgatagta
      721 atctgatatt tgtagcgact atcacacaag ctttagcata tacgattggg attggcgcat
      781 tttatttatt tcatttaaaa gtgatgccat caaggcttcg agctggtttt gcgtatatca
      841 aaaaatattg gcttagattg tcaataacgt atattattgc tctcatctta atttatacat
      901 atgaatatat gacacagttt ctacctaaac atttgcaata tagtgaaaca gccaatgaac
      961 ttgagctaaa taaaatgttt gaagtgcctg catttttacc tgtagcattc ttattaattg
     1021 tgattgtagg tccaatagtt gaagaaatcg tatttagaca tattttaatt ggagaattag
     1081 gtaagaagtt taattttata gctatgagta ttgtttcggt gttcttattt gcatttattc
     1141 atgtgactga tgcaaagtcg ccgtttgagt ttggtccata tctcatacta tccatcatat
     1201 tagttttcac ttatttaaaa tctggaagaa acctaggttc aacaattgca ttacatatag
     1261 caaataattt tgtttctttc gttatatcag taataagtat ttatgcgtag ataaagaggt
     1321 cgggacatta agtttttagg caatgaaaaa agctgatttc tattaattat ttgatagaaa
     1381 tcagcttttt tgatatgcat tttattatga aactgaataa ataaagattc aattagcgca
     1441 tcagtattag gattcactct aaaa
//
LOCUS       DANRDI010000285         1387 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.291, whole genome shotgun sequence.
ACCESSION   DANRDI010000285 DANRDI010000000
VERSION     DANRDI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.291"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1318
                     /locus_tag="PZ377_002843"
     rRNA            <1..1318
                     /locus_tag="PZ377_002843"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          369 a          305 c          402 g          311 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttactatct agttttgaat gtataaatta
     1381 cattcat
//
LOCUS       DANRDI010000286         1384 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.292, whole genome shotgun sequence.
ACCESSION   DANRDI010000286 DANRDI010000000
VERSION     DANRDI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.292"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(201..305)
                     /locus_tag="PZ377_002844"
     CDS             complement(201..305)
                     /locus_tag="PZ377_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359683.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
     gene            complement(468..602)
                     /locus_tag="PZ377_002845"
     CDS             complement(468..602)
                     /locus_tag="PZ377_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359684.1"
                     /translation="MTDKNGHGIAITGIILFVINALQINNDLPISISSKLKKYIHKPD
                     "
     gene            complement(696..953)
                     /locus_tag="PZ377_002846"
     CDS             complement(696..953)
                     /locus_tag="PZ377_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359685.1"
                     /translation="MEIIIEIYMYLRDGIKEHFILKKVRKKFKKLNEINSDIQNMYAE
                     NQGLLETDKTIIDKVLSTDVTNSHDINELYLFLKNYLDKTQ"
BASE COUNT          483 a          197 c          150 g          554 t
ORIGIN      
        1 tgaaattata ttattacttc tacaaatttc aaattgccgt aattgaacgt atatttcttc
       61 ttcaactatt atttcatctt tagcataatc tatatataaa attttatgct attatttaaa
      121 taattcgcta taacttaaca tacgttttcg atataaacct tgttctaaat ctcaataatt
      181 ttttgctgtt ttcatcgtca ttagttaaaa aaataattta actgagtttt tgagacctgt
      241 ttaaattgat gactcaaaac ctttatcccc ttttcactcg gtttaattgc tttaatattt
      301 aacacagtct cattgtaatt ttgctactaa gtttgaaata tttcgattcg aatgtgaaac
      361 atcatttatt attactttcg acagctagaa aaatattgtt aaaccaaaac tataattacc
      421 acttttatat agaactatat ataaatttta actttgagac agaactgcta gtcaggttta
      481 tgaatatatt tctttagttt acttgatata cttattggta aatcattatt tatttgaaga
      541 gcatttataa caaaaagtat aatcccagtt atagcgattc catgaccatt tttatcagtc
      601 aaagattcat caacaaaaac tttttgataa atttatatat ttgtataatt tattatggta
      661 gatatctaca cccgtatcta taacagctac agttatcatt gcgtcttatc caaatagttt
      721 ttaagaaata aatataattc attaatatca tggctatttg taacgtcagt gcttaagacc
      781 ttgtcaatta ttgttttatc tgtttctaag agcccctgat tttctgcata catattttgt
      841 atatcgctgt taatctcatt taatttttta aatttttttc taaccttttt caaaataaaa
      901 tgctctttaa ttccatctct taaatacata taaatctcta ttataatctc cataaatgtc
      961 atatctcctc tctcaaaaaa cattatactc aatatattaa aaataagaaa ggttttttca
     1021 gaatttttag tattttcaga tatttattct taagtatctt attattactt ctttttaacg
     1081 ctagtggaat aattaataat aattgtattt aaatgcaatg ctgttgttct ttcatactta
     1141 caagcaagtc ataagaaatg agaaattaaa ttcttatatg atgaataaat gatgactgca
     1201 gaagaaattg ctaaaaaagg agtggagtga ggcattctac tgttcatatt gttaacaaat
     1261 atcaaaatga taggaacttg aagaatctaa gactgttaaa atcgagacaa taagaaactc
     1321 gttccaaatt tgaatcctat aatgaattcg tctcatgaag aagttattta cttttcatta
     1381 gact
//
LOCUS       DANRDI010000287         1374 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.293, whole genome shotgun sequence.
ACCESSION   DANRDI010000287 DANRDI010000000
VERSION     DANRDI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.293"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(137..898)
                     /locus_tag="PZ377_002847"
     CDS             complement(137..898)
                     /locus_tag="PZ377_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDM6359686.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            917..1051
                     /locus_tag="PZ377_002848"
     CDS             917..1051
                     /locus_tag="PZ377_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359687.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            complement(1149..>1374)
                     /locus_tag="PZ377_002849"
     CDS             complement(1149..>1374)
                     /locus_tag="PZ377_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDM6359688.1"
                     /translation="ATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADA
                     IDFGNKASSLTVQKHGAQASIPLLEEVNQV"
BASE COUNT          434 a          225 c          227 g          488 t
ORIGIN      
        1 gtttatgaaa gtctttaacc aaaattgatg acttactaat caacgataac gatattgtta
       61 agtagatgat tagtgtcaca aagaaattaa agtgatagtc aaacatagat acaaagtaca
      121 gttagtggca ttatatttag tgctcttttt tagcgacaaa agtaatataa ttcatatctt
      181 tacgcaattt agtcatcgtt ttaaacattt tacaaaacat tggtcgattt tcttttttca
      241 aagcattgtt gataatcttt atagttccaa caataccttc gtcataaatt aaaccttttg
      301 gtgtcattaa actcattgga ccagtatgat aatgcacatg attaaaacca gcttgattat
      361 ataaatctaa ccagccaagt ttcgtctgcg gtgagacatt gacattaatt gctgcagata
      421 atgatttaac aacatgtgtg gcatgtgatt cattaacgat gacaatatca tgtgttaaca
      481 agataccccc aggctttaag actcggtagt actcgcgtaa tgctttttcc tttatggcga
      541 tgggtaacat tgttaacatt gcttcattta aaacgatatc gaattgattg tcatcaaagg
      601 gcaatttaac agcattcgct tgttgaactt gaatatatga ttcaagacct gctgctgaaa
      661 tgttttcctg tgctttttct aatgctttct tatttatatc aacgccttga atgtgacagc
      721 catatgtatg agctagataa atagatgttg tgcacatatt acatgccact tctaacactt
      781 gtttatcttg tgaaaatgcc ccttgttgta ttaaccaatc tgttgcttct ttaccaccgg
      841 ggcgtagacg agtttttcct aatttagcta aaaatgtatg accagcttct ttagacatag
      901 cataacctcc ttttaattga taattattat cattaatata gcatatttat atgcgataat
      961 ttggaagtta aaaagattac aaaaaattgt cacttcattt ataactaaac atgagtcaac
     1021 aagcataggt ttagtggaaa attatttcta atagatgatg ttttataaag ataaaaaagc
     1081 acagccatga tatacgaatg ttgcatgtta tatgctaaac cttcatatca tagctgtgtt
     1141 tgattcattt aaacttgatt tacttcttct agtagaggaa tagatgcttg cgcgccgtgt
     1201 ttttgtacag tgagtgagct cgctttatta ccaaaatcaa tagcatctgc taagttatct
     1261 tgcgacttgt ttaagcgact gacaaatgca ccaataaatg tgtcgcctgc agcagttgta
     1321 tcaatcgcat ttactttata agcttcgatg tgttggcttt gatttttagt agca
//
LOCUS       DANRDI010000288         1355 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.294, whole genome shotgun sequence.
ACCESSION   DANRDI010000288 DANRDI010000000
VERSION     DANRDI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.294"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..447
                     /locus_tag="PZ377_002850"
     CDS             <1..447
                     /locus_tag="PZ377_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDM6359689.1"
                     /translation="GKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPDLQVDEK
                     GEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKADREFDE
                     RMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(550..858)
                     /gene="spn"
                     /locus_tag="PZ377_002851"
     CDS             complement(550..858)
                     /gene="spn"
                     /locus_tag="PZ377_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDM6359690.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            1226..>1355
                     /locus_tag="PZ377_002852"
     CDS             1226..>1355
                     /locus_tag="PZ377_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDM6359691.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFA"
BASE COUNT          513 a          174 c          230 g          438 t
ORIGIN      
        1 ggtaaaggta ttcttgataa agaaggtact aaagtaatta aaggtaagac taaattcgca
       61 aatgcagttg tgaaggtaga ctctgaacta ggtgaaggtc aagaattccc tgatttgcaa
      121 gtcgatgaaa aaggtgaatt cagctttgat gtagatcatg ctggatttag attacaaaat
      181 ggagaaacac taaacttcac agtagttgat cctattacag gtgaattatt aagtggaaat
      241 tttgtttcta aaaacataga tatttatgaa tctcctgaag aaaaagcaga tcgtgagttt
      301 gatgaaagaa tggaaaacac acctgcatat cataaattac atggtgataa aattgtcggc
      361 tacgatacta acggattccc gattacctgg ttttatccat taggtgaaaa gaaagttgaa
      421 cgtaaggcac caaaattaga aaaataatta aataaaacag cttaatgatg taatgaaatt
      481 agtgagttaa tcactgactt ctacgtcatt gagctgtttt ttgtgctttg ttacaaagca
      541 ttattgaatt tattttacgt gttcatattt tgaaacatca aagccgtctt gtttagcttt
      601 gttgataatg tctttgattg aatgtagtcc tttatcggcg aagtatgatc ttaagttgtc
      661 ttttgtagct tggtcagcat tcttatctaa taacacatca atataactta attcatgttc
      721 taagaagttt gcatcatcat gtagtacgag tccattttga gaataaactt tcgcatctgc
      781 ttgattacca tatccaacaa cgccagttgc taaaacacct accattgccg tagctactaa
      841 aaccttttta aatttcatat ctatcactcc tctaaaaatt gtaactccat cataacactg
      901 aatattaaga aaattacgtt tattaagtcg atttaataat ttttaataaa tagttaaagt
      961 gacaaatatt gtttaaatgc aattaatctt taatacgatg cttgaggatt tttcctaata
     1021 aaaccttgat ttcaaaaagg gtttaaatca aatgaaacaa taataaaaaa atgacgcaat
     1081 ataataataa gtacaaattt aattaagaaa ttaaattgat tgtatatgta tattttggta
     1141 acgtaaaaga gaaatataca aaataattaa ttatttatat gaaaagagaa tataaatgaa
     1201 gtataaaaca gagagacgtg aagcgatggg atatttaaaa aggtttgcat tgtacataag
     1261 cgttatgatt ttaatatttg cgatagcagg ttgtggcaaa ggtaatgaaa caaaagaaga
     1321 ttcaaaggaa gaacaaatca aaaagagctt tgcga
//
LOCUS       DANRDI010000289         1326 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.295, whole genome shotgun sequence.
ACCESSION   DANRDI010000289 DANRDI010000000
VERSION     DANRDI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.295"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>1326
                     /locus_tag="PZ377_002853"
     CDS             <1..>1326
                     /locus_tag="PZ377_002853"
                     /inference="COORDINATES: protein motif:HMM:NF021909.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDM6359692.1"
                     /translation="VTNLTGYKFNPNAKNFKIYEVTDQNQFVDSFTPDTSKLKDVTDQ
                     FDVIYSNDNKTATVDLMKGQTSSNKQYIIQQVAYPDNSSTDNGKIDYTLDTDKTKYSW
                     SNSYSNVNGSSTANGDQKKYNLGDYVWEDTNKDGKQDANEKGIKGVYVILKDSNGKEL
                     DRTTTDENGKYQFTGLSNGTYSVEFSTPAGYTPTTANVGTDDAVDSDGLTTTGVIKDA
                     DNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKGVKVTLQNEKGEVIGTTET
                     DENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEVDVTITDHDDFTLD
                     NGYYEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS"
BASE COUNT          509 a          217 c          262 g          338 t
ORIGIN      
        1 tgttactaat ttaactggat ataaatttaa tccaaatgca aaaaacttca aaatttacga
       61 agtgacagat caaaatcaat ttgtggatag tttcacccct gatacttcaa aacttaaaga
      121 tgttactgat caattcgatg ttatttatag taatgataat aaaacagcta cagtcgattt
      181 aatgaaaggc caaacaagca gcaataaaca atacatcatt caacaagttg cttatccaga
      241 taatagttca acagataatg gaaaaattga ttatacttta gacactgaca aaactaaata
      301 tagttggtca aatagttatt caaatgtgaa tggctcatca actgctaatg gcgaccaaaa
      361 gaaatataat ctaggtgact atgtatggga agatacaaat aaagatggta aacaagatgc
      421 caatgaaaaa gggattaaag gtgtttatgt cattcttaaa gatagtaacg gtaaagaatt
      481 agatcgtacg acaacagatg aaaatggtaa atatcagttc actggtttaa gcaatggaac
      541 ttatagtgta gagttttcaa caccagccgg ttatacaccg acaactgcaa atgtaggtac
      601 agatgatgct gtagattctg atggactaac tacaacaggt gtcattaaag acgctgacaa
      661 catgacatta gatagtggat tctacaaaac accaaaatat agtttaggtg attatgtttg
      721 gtacgacagt aataaagatg gtaaacaaga ttcgactgaa aaaggaatta aaggtgttaa
      781 agttactttg caaaacgaaa aaggcgaagt aattggtaca actgaaacag atgaaaatgg
      841 taaataccgc tttgataatt tagatagtgg taaatacaaa gttatctttg aaaaacctgc
      901 tggcttaact caaacaggta caaatacaac tgaagatgat aaagatgccg atggtggcga
      961 agttgatgta acaattacgg atcatgatga tttcacactt gataatggct actacgaaga
     1021 agaaacatca gatagcgact cagattctga cagcgattca gactcagata gcgactcaga
     1081 ttcagatagc gactcagatt cagacagcga ttcagacagc gactcagact cagatagcga
     1141 ttcagattca gacagcgact cagactcaga cagcgattca gactcggata gcgactcaga
     1201 ctcagatagc gactcagatt cggatagcga ctcagactca gatagcgatt cagattcaga
     1261 tagcgattcg gactcagaca gtgattcaga ttcagactca gatagcgact cagattctga
     1321 cagcga
//
LOCUS       DANRDI010000290         1325 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.296, whole genome shotgun sequence.
ACCESSION   DANRDI010000290 DANRDI010000000
VERSION     DANRDI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.296"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(179..1150)
                     /gene="nrdF"
                     /locus_tag="PZ377_002854"
     CDS             complement(179..1150)
                     /gene="nrdF"
                     /locus_tag="PZ377_002854"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDM6359693.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
BASE COUNT          409 a          235 c          196 g          485 t
ORIGIN      
        1 tgttggggcc ccgccaacta ctgccaatat aaatagcatt gtacatctta gagcattgat
       61 ttcttactca gactcataat gagttagata cacataaaaa aagaccttca acatgtagga
      121 gacaagacga aacgaatcgc tatttccctt tacatgtgaa ggtcttttaa tttaattgtt
      181 atttgttgtc aaatacaaag tcatcatctt gtaatgcttc tacgtttaat gctaatacat
      241 aaccatcacc ttttactgag aagaagtcat ggtttttagt tgttgtatct aaggcattct
      301 caatgattgg gttaaattca cgttcctcaa aataaggttc aaagcctaag tttgaaagtg
      361 ctttgtttcc attatattta acatagttta gcacatcttc agtgattcca agatcatcgt
      421 ataacatttt tgtgtatgac tcttcattta aatacaagtc atttagcaat ttatacattt
      481 cttgatctgc tttttgtttc tcactttcag atagttcatt tcgtaaatgc tgtgcatcta
      541 aaccggtaaa tacaccatga atagattcat ctaaaagaat tttacgaatg atttcacctg
      601 atgtcgtcat tttcccttga ccagcaagat atagtggata atagaaacct gagaagaata
      661 agaatgtttc taaaaataca ctcgtaactc tggccatata ttggtcgtat atcgaagctt
      721 ctttacccca aagtttgtga taattagcaa caattttatc agatttatat tttaaatggg
      781 gttcctctaa aacccattca tctaataggt agtttgtttc actagatggt aatagtgttg
      841 tgaaaatatg tgaatagctt ttcgcgtgta tttgctccat catcgccata aatgaataaa
      901 ctgctttttt ccttaagtca gtcgtatgta gcataactaa aggcatgcca tcatctgctt
      961 gatgtgtatc taagcctgtt aaaccagcta atgctttttt aaatgtgtct tgttcagctt
     1021 cagataaagt cttccaactt gcaatgtctt ttgatacttt aaattctgtt tcaacccaca
     1081 tttgagatat attttgtctc caaaacatat tcgtcatatc ttcttgtgtg ttccaattaa
     1141 cagctatcat gaatataatt tcctcctgta atgttactca ataattgaca aatacagaca
     1201 tagcagtcta atcatctgct aattcagagc agatgattag ctgtttgtca ttttcaacat
     1261 ttaattgtta gatagaacaa cttgtacatt cttctacact taataattta tttctagtat
     1321 agtaa
//
LOCUS       DANRDI010000291         1306 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.298, whole genome shotgun sequence.
ACCESSION   DANRDI010000291 DANRDI010000000
VERSION     DANRDI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.298"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(108..1184)
                     /locus_tag="PZ377_002855"
     CDS             complement(108..1184)
                     /locus_tag="PZ377_002855"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDM6359694.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
BASE COUNT          412 a          263 c          168 g          463 t
ORIGIN      
        1 caataaacat tgtaaactta atttattacc atgcttcgct tcattgaaag ggattttagt
       61 catgattaac ttttgcatat tgttttcatg attatattca atttttatta atattttggt
      121 acaacgactc tccaaccatt tttatcttct aaagtaccat tttgaatacc agtatagacg
      181 tcgtataatt tttgagtaat ttcaccagtc tcattattat taataacgat ttcacgatct
      241 tcgtatctca atgtacccac aggtgaaata actgctgcag taccactacc aaatacttct
      301 gttaactcac ctttatcata tgattcgaat aattcatcga ttgaaacgcg gcgctcttcg
      361 acttcatatc ctaagttttt agctaattcg ataatagatt tacgtgtaat accaggtaaa
      421 atactgccat tcaactctgg tgtaattact ttgccatttt caacgaagaa aatgttcatg
      481 ctaccaactt cttcgatata tttctgttca acaccatcaa gccataatac ttggtcataa
      541 cctaatttat ttgcattagt ttgtgctaat aaacttgccg catagttacc tgcaactttt
      601 gcaaagccta caccgccacg aacagcacgc acatattcat cttctacata gattttagtt
      661 ggttttaaag tttcaccacc ataatatgca cctgaaggag ataaaataat taataattta
      721 tactgatgtg atgcaccaac gccaagtgcc ccttctgttg caaaaacaaa tggacgaata
      781 tataatgatt gaccttcccc ttcaggaatc caatctcttt caatatcaac taattgtttt
      841 agcccctcta acaattctgc ttcgtctact tgaggcattt ctaatcgtgc taacgagtta
      901 ttaagacgct taaaattttc ttcaggacgg aaaagtgcaa cttccccatc tcttttatat
      961 gcttttaatc cttcgaatac cgattgacca taatgaacac cttgtgcagc aggtgaaatt
     1021 tcaataggac cataaggtac tatcttcaaa tcatgccatc ctttatctgc atcataatca
     1081 taactcaaca tataatcagt aaaatattta ccaaaaccta gttgagatgt atttggtttt
     1141 tgttttaatg tttctcgtcg ttcaacttta actgcttgtg acatggtgat tgcctcctaa
     1201 taatattgta taagaatttg tttaacttaa attataacaa tccatatttt gctgttcaac
     1261 aaattttcta aaaattcaaa attaattaac agatttctag aaagac
//
LOCUS       DANRDI010000292         1282 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.299, whole genome shotgun sequence.
ACCESSION   DANRDI010000292 DANRDI010000000
VERSION     DANRDI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.299"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..315
                     /locus_tag="PZ377_002856"
     CDS             <1..315
                     /locus_tag="PZ377_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDM6359695.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDNDSDSDSDSDSD
                     AGKHTPAKPMSTVKDQHKTAKALPETGSENNNSNNGTLFGGLFAALGSLLLFGRRKKQ
                     NK"
     gene            682..>1282
                     /locus_tag="PZ377_002857"
     CDS             682..>1282
                     /locus_tag="PZ377_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HDM6359696.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDE"
BASE COUNT          544 a          180 c          216 g          342 t
ORIGIN      
        1 tcagatagcg actcagactc agatagcgac tcagactcgg atagcgattc agattcagac
       61 agcgactcag attcagatag cgattcggac tcagacaacg actcagattc agatagcgat
      121 tcagattcag atgcaggtaa acatactccg gctaaaccaa tgagtacggt taaagatcag
      181 cataaaacag ctaaagcatt accagaaaca ggtagtgaaa ataataattc aaataatggc
      241 acattattcg gtggattatt cgcggcatta ggatcattat tgttattcgg tcgtcgtaaa
      301 aaacaaaata aataatatga ttaacttaac caggtccatg tggcctggtt ttttctgttt
      361 agaaattccc agatataaaa caaaaaatct ttaaggaata gatacaattt aatttgttac
      421 agaaatgtaa ttgtctatga attagaaaag ctaagatatt aatttcaagg gcaaataata
      481 ctatttttta tgataattaa ataaagatta caaaactcat aaaaactttt atttaaacac
      541 aaatgttaaa taacgtttta cgataaagaa aaataattaa agtattgtgc tttatctaaa
      601 aatgtaatac tatattgata tacatttttg tatttaaaac aattgtatta tttaaaaatt
      661 tgatgaatta ggagtaatct aatgctaaac agagaaaata aaacggcaat aacaaggaaa
      721 ggcatggtat ccaatcgatt aaataaattt tcgattagaa agtacacagt gggaacagca
      781 tcaattttag taggtacaac attaattttt ggtctgggga accaagaagc aaaggctgca
      841 gaaagtacta ataaagaatt gaacgaagcg acaacttcag caagtgataa tcaatcgagt
      901 gataaagttg atatgcagca actaaatcaa gaagacaata ctaaaaatga taatcaaaaa
      961 gaaatggtat catctcaagg taatgaaacg acttcaaatg ggaataaatt aatagaaaaa
     1021 gaaagtgtac aatctaccac tggaaataaa gttgaagttt caactgccaa atcagatgag
     1081 caagcttcac caaaatctac gaatgaagat ttaaacacta aacaaactat aagtaatcaa
     1141 gaagcgttac aacctgattt gcaagagaat aaatcagtgg taaatgttca accaactaat
     1201 gaggaaaaca aaaaggtaga tgccaaaact gaatcaacta cattaaatgt taaaagtgat
     1261 gctatcaaga gtaatgatga aa
//
LOCUS       DANRDI010000293         1281 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.300, whole genome shotgun sequence.
ACCESSION   DANRDI010000293 DANRDI010000000
VERSION     DANRDI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.300"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..329)
                     /locus_tag="PZ377_002858"
     CDS             complement(<1..329)
                     /locus_tag="PZ377_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDM6359697.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTV"
     gene            complement(345..656)
                     /locus_tag="PZ377_002859"
     CDS             complement(345..656)
                     /locus_tag="PZ377_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359698.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            complement(743..1093)
                     /locus_tag="PZ377_002860"
     CDS             complement(743..1093)
                     /locus_tag="PZ377_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359699.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
BASE COUNT          369 a          204 c          223 g          485 t
ORIGIN      
        1 actgtgtcac tttcactaat tgctttcatt aagtgtatat ctgtgtgctc atcataaggc
       61 tctttgatat gtttaaggtt ctctggtgtt ttaatattag agaaaagatt aacgagataa
      121 acagcgccat attgctcaga attagctaat tgattgagga tgagaaccgt agtaagatcg
      181 agtgataata caccatctaa atgaagatac atcgttatca cagtacaagc cggtttcttg
      241 tcatcccata tctttttgag taagtagcga tgttgttcat catcgctaaa tatcgcttct
      301 gtgtgtatcg tacttttgat tgtattcata tatcgtcact tcctttagta ttcttctggc
      361 aaaagcatca cataataaaa agcgtctaca tcatcttctc ggatgacgta gactttctta
      421 ggtaatgcat tttgattttt tatatagttt gtatagtgat attccaattt gtatgcgggt
      481 tgttcttgtt catgtgtaat tgacaacata ttatcatctt cttgtagttt gaaaatgtgt
      541 aggtaatctg tattaagttg attgtctcga tcttttacca tgttccaaag taagatttga
      601 aggtctagag atagttgttc actaatgcct cttgtgatgt atcgattgat tttcatacta
      661 ttttcctcca ttttgctttt ctttcatgat gtcaatcact tcgttaatga ctgtaacaga
      721 tatttgtgcc actttgatcc aattattcat ggttattccc tccttctttt agtaaatgac
      781 gttcatcgat aatcgtattt ttagtatctg tgaggtatag aaagtccata tcaaaatgat
      841 ccagataacc aatactgatg agttggttat tagaatacat tagaaatgga tagatactta
      901 gttcatgtag ctcattatta tagtaggtat aagtttcaag tataagatgt gcaagtgggg
      961 aatcattaat aaaacgtttg ggtagaatat tttctgccgc ttcctccagt gtttcacatt
     1021 cccatgtttc attgttagat aattggaata agcgagtcat atattgtttg agttcttgag
     1081 tagttgtttt catatcattg cctccctaga tagtgtgata gtgatgtact tcatatacat
     1141 cattgagata atatatattt gatttgtcat ttattactga tcccgttgac aatatgagaa
     1201 aattccatat aaaaaaccgc tacaaacctt ggtatgacaa ggaaatcccg aaattccgcc
     1261 tattttgacg aacaatcaac t
//
LOCUS       DANRDI010000294         1266 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.301, whole genome shotgun sequence.
ACCESSION   DANRDI010000294 DANRDI010000000
VERSION     DANRDI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.301"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..176
                     /locus_tag="PZ377_002861"
     CDS             <1..176
                     /locus_tag="PZ377_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDM6359700.1"
                     /translation="IPTEKAPILKMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDS
                     LNFQPAKKNKDDE"
     gene            195..1002
                     /locus_tag="PZ377_002862"
                     /pseudo
     CDS             195..1002
                     /locus_tag="PZ377_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            1048..>1266
                     /gene="lpl4"
                     /locus_tag="PZ377_002863"
     CDS             1048..>1266
                     /gene="lpl4"
                     /locus_tag="PZ377_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HDM6359701.1"
                     /translation="MMGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIK
                     KSFAKTLDMYSIKNLEELYDKEGYRDGEF"
BASE COUNT          543 a          127 c          250 g          346 t
ORIGIN      
        1 acattcctac tgaaaaagct ccaatattaa aaatgcatat tgatggggac ttaaaaggca
       61 gttccgttgg atataaaaag ttagaaatag acttttcaaa agaagaaaat agcgaattat
      121 cagtagtcga ttcattaaat tttcagcctg ccaaaaaaaa taaagatgat gaatgatagg
      181 gaggatgtga aacaatgtgg tcaataaaaa gaattggaat gtacacaaat gttataattt
      241 tgagcgtttt tataataggt tgtgatagtt caagcaataa tgcagaaaat caaagagaag
      301 actcaaaaga agaacaaatc aaaaagagct ttgcgaaaac gttagatatg taccctatta
      361 agaatctcga ggatttatat gacaaagaag gatatcgtga tggtgagttt gaaaaaggcg
      421 ataaggggac gtgggtattg tattcagcta tggtatcaca accaaaaggc gagagtttaa
      481 aatcacgagg aatgatatta aagttagata gaaataagag aactgctaaa ggaagttata
      541 ttattagaga attgaaagaa gataaaaatc atgatgttca aaaaaatgaa aagaaatatc
      601 cagtgaaatt ggtgaataat aggatagttt tgataaaaga tgttaaagac aaaaagttaa
      661 aaaaatgaaa tagagtcgtt tgaattattt tcacaatatg gaaactttaa tcattttgat
      721 cggaatgaga ttactaatat ttcatataat cctaatgctc ccaattactc tgcagaatat
      781 aaaatgaaga aaaatgacag aaacattcaa cagttgaaaa agagatttaa tctaaaaact
      841 agcaagacac caaaattatt gtttaaggga tctggagata taaaggggtc ttctgtagga
      901 tataaggaaa tagaaatcat atttagtaga agtaaagaag aaagcattta ttatgttgac
      961 agcgttgagt tcgttccaag taacaaaata acaaaaagtt gatattagtc aacaaaagca
     1021 aatattgaga ataaagagag ttgtgaaatg atgggttatt taaaaagact tgtattgtat
     1081 atagttatta tggttatgag tgtttttata ataggttgtg ataaatcaag cgatactgca
     1141 gaaaaatcaa aagaagactc gaaagaaaca caaatcaaaa agagttttgc gaaaacgtta
     1201 gatatgtatt caattaagaa tctcgaggaa ctatatgaca aagaaggcta tcgagatggc
     1261 gaattt
//
LOCUS       DANRDI010000295         1265 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.302, whole genome shotgun sequence.
ACCESSION   DANRDI010000295 DANRDI010000000
VERSION     DANRDI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.302"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..1255
                     /gene="pckA"
                     /locus_tag="PZ377_002864"
     CDS             <1..1255
                     /gene="pckA"
                     /locus_tag="PZ377_002864"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDM6359702.1"
                     /translation="GSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFT
                     IVSAPHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQD
                     IMSMHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGC
                     YAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDN
                     IVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPE
                     PSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTR
                     QMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLI
                     QRFEKNFEKFGEKVEHIAEKGSFNK"
BASE COUNT          443 a          216 c          236 g          370 t
ORIGIN      
        1 tggtagcgat aaagatacaa tgttaaaact tacagtcatc aatgaattag catggcataa
       61 tttatttgct aaaaatatgt ttattagacc tgaatcaaaa gaagaagcta caaagattaa
      121 acctaacttc actatcgttt ctgcaccaca ttttaaagca gatccagaag ttgatggtac
      181 taaatctgaa acctttgtca ttatttcatt taaacacaaa gtcattttaa tcggcggtac
      241 tgaatacgct ggtgaaatga aaaaaggtat cttctctgta atgaattatc tcttaccgat
      301 gcaagatatt atgagcatgc attgctcagc aaacgttggt gaaaaaggcg atgttgcatt
      361 attctttggt ctatctggca ctggtaaaac aaccttatcg gctgacccac accgtaaact
      421 aatcggtgat gatgaacacg gctggaataa aaacggggtc tttaatatcg aaggtggctg
      481 ctatgcaaaa gcaattaatc tttccaaaga aaaagaacca cagatttttg acgcaatcaa
      541 atatggtgca attttagaga acactgtagt tgcagaagat ggttcagtgg actttgaaga
      601 caatcgttat acagaaaaca cgcgtgccgc ttatccaatt aatcacattg acaatattgt
      661 agtaccatct aaagcagcac atccaaatac aattattttc ttaactgcgg atgcatttgg
      721 tgttattcca ccgatttcaa agttaaataa agaccaagca atgtatcatt tcttgagtgg
      781 tttcacttct aaattagctg gtacagagcg tggtgtgaca gaacctgaac catcattctc
      841 aacatgtttc ggagcaccgt tcttcccgtt acaccctact gtttacgctg atctattagg
      901 tgaacttatc gatttacatg atgttgatgt ttatcttgtt aatactggat ggactggcgg
      961 aaaatatggt gtaggacgta gaatcagctt acattacaca cgtcaaatgg taaaccaagc
     1021 gatttctggc aaattgaaaa atgcagaata tacaaaagat agtacgtttg gtttaagcat
     1081 tcctgtagaa attgaagatg taccgaaaac aattttaaat ccaattaatg cttggagcga
     1141 caaagagaaa tataaagcac aagcagaaga tttaattcaa cgttttgaaa agaacttcga
     1201 aaaatttggt gaaaaagttg aacatattgc tgaaaaaggt agcttcaaca aataaatttg
     1261 aatac
//
LOCUS       DANRDI010000296         1243 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.303, whole genome shotgun sequence.
ACCESSION   DANRDI010000296 DANRDI010000000
VERSION     DANRDI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.303"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..148)
                     /locus_tag="PZ377_002865"
     CDS             complement(<1..148)
                     /locus_tag="PZ377_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDM6359703.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
     gene            complement(160..660)
                     /locus_tag="PZ377_002866"
     CDS             complement(160..660)
                     /locus_tag="PZ377_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDM6359704.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            complement(1017..1130)
                     /locus_tag="PZ377_002867"
                     /pseudo
     CDS             complement(1017..1130)
                     /locus_tag="PZ377_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
BASE COUNT          419 a          233 c          114 g          477 t
ORIGIN      
        1 ctggtttagt ataattaaaa atgacttccc cagcttgatc agttacataa gctattgtaa
       61 atacttgctc ccattctact ggtatcattc cactgatctc acttgcaatt ttattgtaca
      121 tttgacttag tttctcttcg aaagtcatgt tatctccccc tatagttcag cttcatcttg
      181 ctctttaaca tattgatcga tttctttaac tttattaatt tcatattccg tttctggtaa
      241 aacaccaaac tttttgtaca tataataatt ttcttttgct attggaccaa attctgaatt
      301 catccaatca acataatcaa atacaacatt caatttacca tctcttgtaa agtcaaattc
      361 acatgatgtc catggttcta aatcttcttc tttaaatgcg tttcttaact tcttaaacaa
      421 tctatataaa tccgtccaca aatcataaaa tactttttca gagacattat actctctcgg
      481 gatatatgtg taataattca attcatcgct acctggttta gtataattaa aaacgatctc
      541 tccacctcta tcatttacat aagctatcgt aaatacttgg tcccattcta ctggtatcat
      601 cccactaatc tcattcgcaa tcttgttgta tatttcactt agtttttctt cgaaagtcat
      661 gttatcgccc ctatattttc aaaatagata ttggtttcat ttccgttaaa taaaaagcac
      721 taaacaacac ctctacgtcg cttaatgctt cttcaaatta atattactca ttttaaacct
      781 aaatacacta atggaactaa aataactgca actattgtta cgataactgg aattctaaat
      841 aatttcgagt ctttgcagta ttatattatt tgtaaataat agccctaaaa ctcctactca
      901 ttattttgtc tttctattta tttaactatt tataattata gcaaaaaaga tattaatagt
      961 atatactttt ttgccatttc gtaaataaaa tttaccagca cctatatgta atacaattat
     1021 aataatcttg ataaaattat attcagaata aataaccaac aattgaaaaa taatagattt
     1081 attacaacaa aagcaaacgt tccttttcta attctaataa tcaaatccca ttaatatcaa
     1141 tccactcaaa ttctttcaca tcattgagta taaaataata tggtgatata cgttgaagtt
     1201 gattttctta tttaagtata ctccaacagt attttataaa att
//
LOCUS       DANRDI010000297         1181 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.304, whole genome shotgun sequence.
ACCESSION   DANRDI010000297 DANRDI010000000
VERSION     DANRDI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.304"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            236..1168
                     /locus_tag="PZ377_002868"
     CDS             236..1168
                     /locus_tag="PZ377_002868"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDM6359705.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
BASE COUNT          435 a          154 c          248 g          344 t
ORIGIN      
        1 attccaatat atcattgtag agcttaggtc attgattttt ggctcggact tttatggcga
       61 tatgaaccat gtaaattaag caagcaataa attaatgatt gatattgact tgtaaaataa
      121 taacaataat gaacaattaa tatttatttt agcttttcaa tgtagattgg tgttatattt
      181 ttgatatgat aagaagagat gtaagagtag ggataaatac aattgaggtg aacccatgtt
      241 aacgacagaa aaactagttg aaacattaaa gttagattta atcgctggtg aagaaggact
      301 atcgaagcca attaaaaatg ctgatatatc aagaccgggc ttagagatgg caggttattt
      361 ttcacattat gcgtcagata gaatacaact attaggaaca acggaactat cgttttacaa
      421 tttattacca gataaggatc gcgcaggtcg tatgcgtaaa ctatgcagac cagaaacgcc
      481 tgcaattatt gtgacacgtg gattgcagcc accagaagaa ttagttgaag ctgcaaaaga
      541 attaaatacc ccacttatag ttgctaaaga tgcgactaca agtttaatga gtcgcttaac
      601 aacgttttta gagcatgcac ttgcaaagac gacatcttta catggtgttt tagtagatgt
      661 ttacggtgtt ggtgtactaa ttaccggtga ttcaggaata ggtaaaagtg agactgcgtt
      721 ggaattagtt aaacgtgggc atagattagt agcagatgat aatgtagaaa tacgtcaaat
      781 taataaagat gaactaatag ggaaaccacc aaagttaata gaacatctat tagaaatacg
      841 tggactaggt attatcaatg ttatgacttt atttggcgcg ggttcaatat taactgaaaa
      901 acgaattaga ttaaatatta atttggaaaa ctggaacaag caaaagttat atgaccgcgt
      961 aggtcttaat gaagagacgc taagtatttt agatactgaa atcactaaaa aaacaatacc
     1021 tgtaagacct ggtagaaatg ttgcggtaat tattgaggtc gctgcaatga actatcgatt
     1081 aaatatcatg ggcattaaca cggccgaaga atttagtgaa agattaaatg aagaaattat
     1141 caagaacagt cataagagtg aggagtaggt tgaatgggta t
//
LOCUS       DANRDI010000298         1178 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.305, whole genome shotgun sequence.
ACCESSION   DANRDI010000298 DANRDI010000000
VERSION     DANRDI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.305"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            23..988
                     /locus_tag="PZ377_002869"
     CDS             23..988
                     /locus_tag="PZ377_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDM6359706.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
BASE COUNT          415 a          162 c          232 g          369 t
ORIGIN      
        1 aagagacaaa ggagatattt ctatgaaaaa aattatgatt actggtgcat taggacaaat
       61 tggtacagaa ttagttgtta agtgcagaga aatttatggg acagataatg ttcttgctac
      121 agatattagg gaacctgaag cagactcacc tgtacaaaat ggaccatttg aaatcttaga
      181 cgtaacagat cgtgaccgta tgtttgagtt agttagggac tttgaagcgg atagtctaat
      241 gcatatggca gcattattat cagcaactgc tgagaaaaat ccaattctag cttgggattt
      301 aaatatgggt ggattaatga atgcattaga agctgcaaga acttataatt tgcacttttt
      361 cacaccaagt tcaattggtg catttggaga ctcaactcct aaagttaata cgccacaagt
      421 aacgattcag caacctacga caatgtatgg tgtaaataaa gtagctggag aattattgtg
      481 tcaatactat ttcaaacgtt ttggtgtaga tacaagaagt gttagattcc caggtttaat
      541 ctcgcatgtt aaagagccag gtggcggtac tacagactat gctgttgaaa tatacttcaa
      601 agcagtaaga gagggtcatt atacaagctt catagataaa ggcacgtata tggatatgat
      661 gtatatggat gatgcaattg aagcaattat taaacttatg gaagcagacg acgctaaatt
      721 agaaactaga aatggttata atttgagcgc aatgagtttt gatccagaga tggtaaaaga
      781 agcaattcaa gaatactatc ccaattttac attagattac gatgttgatc ctattagaca
      841 aggtatcgct aatagttggc cggattctat tgatacaagc tgttcacgtg gcgaatgggg
      901 atttgatcct aaatatgatt tagcgagcat gactaaatta atgttagaag ctattgaaca
      961 aaaagatact gttaaaaata ataactaatc atttccattc actttaatac acggaatgat
     1021 attttaaatt actctttatt ttaataaact agtgcatgaa ttctaatatt attcattata
     1081 cttattgaat tcgcgagctt agttcatttt aaagtaaaga gttttttgta tgtaaagtat
     1141 attagtaaaa cacagattac ttgtctctgt gaaattaa
//
LOCUS       DANRDI010000299         1176 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.306, whole genome shotgun sequence.
ACCESSION   DANRDI010000299 DANRDI010000000
VERSION     DANRDI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.306"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            103..451
                     /locus_tag="PZ377_002870"
                     /pseudo
     CDS             103..451
                     /locus_tag="PZ377_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            462..656
                     /locus_tag="PZ377_002871"
     CDS             462..656
                     /locus_tag="PZ377_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359707.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            697..1047
                     /locus_tag="PZ377_002872"
     CDS             697..1047
                     /locus_tag="PZ377_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359708.1"
                     /translation="MLEMISNGKMTMKLNNVKQKRHILCTNEYNNKKNNSSLLPSYTI
                     IDSNESEKMTKKEFIDIPVLFDDEGNFRIKQVIDYKKIIGKSYVNGKYIETKLGKVHY
                     SKTGFHVVPYIKKE"
     gene            1054..>1176
                     /locus_tag="PZ377_002873"
     CDS             1054..>1176
                     /locus_tag="PZ377_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HDM6359709.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSI"
BASE COUNT          511 a          100 c          205 g          360 t
ORIGIN      
        1 ggcaattggc gggacaagtt ctttaaagag cgtgaaggta aatatcaagt agaagtaaaa
       61 gaagcataat tacaggaaaa agctaaaaac cagatgaaag aaatgattaa aaatggtaaa
      121 ataaaaatag aaataaattg cggaaaaata aaatagatat atgttaggtc atcacctata
      181 taatgaaaat aaaaaaagag ccattttaaa taataagaaa ttgcctagct atacaatact
      241 ttctatagat ttattgaatg aattgttaag agaaaaaatg tcaacaggca atctaatatt
      301 aagtgatgag cgatttgatt tgaaagagat tattaatttt aatcaaatta ttggaaaaat
      361 acatatcgaa aatgtgtata ttgaaaccag aaagggaaaa gtgcattatt cgaagacagg
      421 tgctcatata gtaccttata ttgataagta ggtgaaaagt attgagaatt gaagatgcat
      481 atcgtaaaga tgttattatc acgcttttga ataatgaaga atacgaaggg tttgtaactg
      541 actatgaaaa tgaattcgag agcgaaacag gaaatcttgt tgtagatata cagactgatt
      601 ttgctgttta ttcgtttgat gaaactgaga taaaaagtat tagattatta aaataattac
      661 aaaatagcat cctttctaca aaaagctaaa aaagaaatgc ttgaaatgat aagtaatggt
      721 aaaatgacga tgaaattgaa taatgttaag caaaagcgtc atattttatg cacaaatgaa
      781 tataataata agaaaaataa ttcatcttta ttaccaagtt atactataat agattctaat
      841 gaaagtgaaa agatgactaa aaaagaattt attgatattc cagttttgtt tgatgatgaa
      901 gggaatttta gaataaaaca agttatagat tataaaaaga taataggtaa atcttatgtg
      961 aatggtaaat atattgaaac gaaattaggt aaggttcatt attctaaaac tggttttcat
     1021 gtagttccgt atattaaaaa ggagtgatta aatatgaatt tacagtcata cataggcaaa
     1081 ttagtcaaat taacgcttac taataataaa atattaattg gaaaagtgat agactttgat
     1141 gataaagttg ataattttga tggttataat tcgata
//
LOCUS       DANRDI010000300         1117 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.307, whole genome shotgun sequence.
ACCESSION   DANRDI010000300 DANRDI010000000
VERSION     DANRDI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.307"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..949
                     /locus_tag="PZ377_002874"
     CDS             <1..949
                     /locus_tag="PZ377_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HDM6359710.1"
                     /translation="RPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFNVEEAQIS
                     CILNELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFH
                     NNMINLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAG
                     RLNSKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSN
                     AIKAGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYE
                     SILQDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
BASE COUNT          451 a          133 c          202 g          331 t
ORIGIN      
        1 aagaccagca ttagaattat atgatattag tataaattta ccagatactt ttagcccaaa
       61 agaaggtaaa aagattgaaa tagcattaaa agacgactgg agtatattta atgttgagga
      121 agctcaaata agttgtattt taaatgaact ttttaaagat gatgaaaatt tagatagacg
      181 agtgttggaa gatttagtag gcgctaaaat cataaacgat aaaaaatata caaataaaaa
      241 tctaattgta gcaaataatg actgggatgg attttgtgag agcttgaagt ataaaaatag
      301 atttcataat aatatgatca atttagagaa tttagcgttc tttctcgata tcactactaa
      361 ttactatagt attgaggaat ttagagaaag atttgaacca ttatatagat ctagaatagt
      421 taagttcatt acagagaaaa gtgaactaat gtcaccacca aagggatttg caacggcagg
      481 aagattgaat tctaaatgga taagcgtatt ataccttagt acaaaagaac aggtcagtat
      541 agaagaagtt aaacctaagc ataatgacat tatttatata ggtaaggtta agttacaaaa
      601 aaacgtgaca aaagagaaat tgaaaattgc gaatttaact aatttaacta gtaatgcaat
      661 caaagctggc gatgatggat ttagaaagta ttttgtaaat taccaaacat taaaaaagat
      721 tcataaagga ataacaaatc caagcgatga acaaggaata gattatttgc catttcaata
      781 tttagctgat tatattcgaa gccttaaata tgacgggata atgtatgaga gcatattaca
      841 agatggtact tttaactttg tgttttttga tcaaagttta tttgaatgtg ttaattatga
      901 aagaaaaaga gtaagtgacg ttaaatatac attgactaag ctaagttgac acactcaaaa
      961 atctgaatat tctaataaaa tataaataat cccccgcact tcacatagtg gcgggggatt
     1021 tattctggcg ttaaaatgcc caaagtatgg gggtgactct taaatcatgg caaagcacct
     1081 aaaacttgat atggtgccga gccctaactt agcacaa
//
LOCUS       DANRDI010000301         1058 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.308, whole genome shotgun sequence.
ACCESSION   DANRDI010000301 DANRDI010000000
VERSION     DANRDI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.308"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(19..>1058)
                     /gene="brnQ"
                     /locus_tag="PZ377_002875"
     CDS             complement(19..>1058)
                     /gene="brnQ"
                     /locus_tag="PZ377_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDM6359711.1"
                     /translation="EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSL
                     LTPLLLITILAMIIKGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIA
                     FSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQ
                     LKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPK
                     VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRI
                     SQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIF
                     VKQDPIKYQQE"
BASE COUNT          381 a          172 c          159 g          346 t
ORIGIN      
        1 cctcttttat attattcgtt attcctgttg atatttaatt ggatcttgtt ttacaaatat
       61 gccgactaga tagcctaata tcgttgcaat aattgctact gggaaccact ctaaagaata
      121 cgctcttaga ggcaatgatt ctataaagtt aattttcagc caacctaact tactaataac
      181 actgaaaatc gacaatataa atacgataat aactggaatt tgttgtgaaa tgcgttttgt
      241 cggtatgaat ttggcaatta aaattaataa tacaacagtt attgctactg ggtatacaat
      301 gcttaatacc ggaattgaca ttgagataac agcatttaaa ccttggttag caataataaa
      361 actcattaaa atgaaaacta atacaaatgc tttgtatgat actttaggta cgattctatg
      421 gaaatattca gaaactgcaa caataagccc gcatgctgta gttagacatg ccagcgccac
      481 aatgatgccc aataaatatt ttccgaatga accaaatcct gttgaagcca ttgtcgttaa
      541 taaatatgtc ccaatgtttc gatctttgga tttcaattga tctaacgtca tgtcacttac
      601 tggcatatga ttaccaatat aacctaatga aatatatatg aaaattaaag ctacggctgc
      661 aattaaacca gcagtcaaag tttgtttgaa tatttgattt gttttagtaa tgcctgttag
      721 ttttactgca ttaacaacaa tcattgaaaa agcaattgct gcaatggcat ccattgttaa
      781 atagccttgt gtaaagcctt cagcaaaact tgaaaaatta gaatgatata gtgcttcatt
      841 gccctttcca gcactattac cgctaaagtc taagtatcct ttaataatca tcgctaaaat
      901 agtaatcaat aataatggtg ttaataatga accaatacga tcgattaact tagatggatt
      961 taaacaaata tacaaaacga ctatgaagta gataatcgta aatataaata aagcgatact
     1021 actattgcta tgtataattg gtgtaattgt catttcaa
//
LOCUS       DANRDI010000302         1039 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.309, whole genome shotgun sequence.
ACCESSION   DANRDI010000302 DANRDI010000000
VERSION     DANRDI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.309"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..247)
                     /locus_tag="PZ377_002876"
     CDS             complement(<1..247)
                     /locus_tag="PZ377_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme transporter IsdA"
                     /protein_id="HDM6359712.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKS"
     gene            456..>1039
                     /gene="isdC"
                     /locus_tag="PZ377_002877"
     CDS             456..>1039
                     /gene="isdC"
                     /locus_tag="PZ377_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDM6359713.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAG"
BASE COUNT          373 a          144 c          185 g          337 t
ORIGIN      
        1 gtgacttctc tgaagagcca tctttttgca cttggaaatt aattggttgt gatgttgctt
       61 gagaaacttg tgtgctttga ttattagttg cgttcgtagc ttctgttgcc gcattgactt
      121 gttggctgtc tgcgcctatg tatacaaggg aacctaaaat gatagatgct gtacccattg
      181 taatcttttt catagctgat gaacgttgtt ctgattgata cttactgttt aaataatgtt
      241 ttgtcatgtt gttttcctcc taaggataca aattatgtgt tgttatatga gatgattaac
      301 cattgcaatt gataatggtt atcaattata ctagcacatc tattttcgta taacaataat
      361 aaattgatta taaaataaat attgacaatg ataatcatta ttatttatga ttttactaaa
      421 gacttaaaag caatcataaa aaggaggatt atgttttgaa aaatatttta aaagttttta
      481 atacaacgat tttagcgtta attatcatca tcgcgacatt cagtaattct gcaaatgccg
      541 cagatagcgg tactttgaat tatgaggttt acaaatacaa taccaatgac acgtcaattg
      601 ctaatgacta ttttaataaa ccggcaaagt acattaagaa aaatggtaaa ttgtatgttc
      661 aaataactgt caaccacagt cattggatta ctggaatgag tatcgaagga cataaagaaa
      721 atattattag taaaaacact gccaaagatg aacgcacttc tgaatttgaa gtaagtaagt
      781 tgaacggtaa aatagatgga aaaattgacg tttatatcga tgaaaaagta aatggaaagc
      841 cattcaaata tgaccatcat tacaacatta catataaatt taatggacca actgatgtag
      901 caggtgctaa tgcaccaggt aaagatgata aaaattctgc ttcaggtagt gacaaaggat
      961 ctgatggaac gactactggt caaagtgaat ctaatagttc gaataaagac aaagtagaaa
     1021 atccacaaac aaatgctgg
//
LOCUS       DANRDI010000303         1028 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.310, whole genome shotgun sequence.
ACCESSION   DANRDI010000303 DANRDI010000000
VERSION     DANRDI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.310"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>1028
                     /locus_tag="PZ377_002878"
     CDS             <1..>1028
                     /locus_tag="PZ377_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDM6359714.1"
                     /translation="ISDNVIPYDAFRNNFLDVLLIKMPQKNHPLNIWALHKLLKQCNL
                     GKRDFLWTQYISINNEKVFEIINWLFSNYKKLDEETAEKYMIFLTWIFSATNNKLRDL
                     GTKSLVKLFKTFPTKIIGLLKLFENNNDPYIVERLYASVLGATLRIDICEIHIEIANY
                     IYEEIFDKEMVYPHILMRDYARQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNL
                     NIDDYIKSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGF
                     KSDQDLSNIALMRIFEMGYDAKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILA
                     KLVDKFPD"
BASE COUNT          411 a          100 c          159 g          358 t
ORIGIN      
        1 tatatccgat aatgttattc cttatgatgc atttagaaat aattttttag atgtcttatt
       61 aataaaaatg cctcaaaaaa accatccgtt aaatatttgg gctttacata aattattgaa
      121 acaatgcaat ttaggaaaaa gagatttttt atggactcaa tatatatcta taaataatga
      181 aaaggttttt gaaattatta attggctatt tagtaactat aagaaattag atgaagaaac
      241 tgcggaaaaa tatatgattt ttttaacgtg gatattttct gcaactaata ataaattaag
      301 agatctcggt actaaatctt tagtaaaatt attcaaaaca tttccaacta aaattattgg
      361 tttattaaaa ttatttgaaa ataacaatga tccatatata gtcgaaagat tatatgcttc
      421 tgttttaggg gcgacacttc gaatcgacat ttgtgaaata catatagaga ttgctaatta
      481 tatttatgaa gaaatatttg ataaagaaat ggtatatcct catattttaa tgagagatta
      541 tgctaggcaa acaattgaat atattagttt atcaaaagat atatctaata taaatttaga
      601 aaaaattagg ccaccttata aaagtaattg gtataaaaaa gaatattcga atttaaatat
      661 agacgattac ataaaatctt taaaaaacaa attagatagc catttacatt tttctatcga
      721 taaaattaaa aactcaatga caacggaata tggaagagga actggggcat atggcgattt
      781 tggcagatat gtatttggtt atgctgtacg aaactgggta aagggtttta aaagtgatca
      841 agacttaagt aatatagcat taatgaggat ttttgaaatg ggttatgatg caaaattaca
      901 tggtgaattt gatatgtggg ttaatcgtta tgataacttt aataattcaa ttgaaagaat
      961 tagcaaaaaa tatcaatgga ttgcttacta tgaaatttta gctaaacttg tagataaatt
     1021 tcctgatg
//
LOCUS       DANRDI010000304         1014 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.311, whole genome shotgun sequence.
ACCESSION   DANRDI010000304 DANRDI010000000
VERSION     DANRDI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.311"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            127..942
                     /locus_tag="PZ377_002879"
     CDS             127..942
                     /locus_tag="PZ377_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HDM6359715.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETELTYN"
BASE COUNT          382 a          109 c          198 g          325 t
ORIGIN      
        1 taaaaaacat gggcttaaag ggttaattta tatagtatta ctgcaaacat tgatttgggt
       61 caaaaattag gggtattaaa aaaatgaatg aatcataatt ttatcaagct gattggagag
      121 gttaaaatgc attatataaa atttattgag tcaaaagata atacaaaact ttatatgaaa
      181 gtgaatgata ttcaagatgc aaaagcgaat atcattatag ctcatggtgt ggcagaacat
      241 ttagatcgtt atgatgagat aacagcatat ttaaatgaag cgggttttag tgttattaga
      301 tatgatcaaa gagggcatgg tcgttctgaa ggcaagcgtg ccttttatag caatagtaat
      361 gaaattgtcg aagatttaga tgcgataata aattatgtga agtcaaactt tgaaggtaaa
      421 gtttacttaa tcggtcatag tatgggtggt tatacagtca ctttatatgg aacgaaacat
      481 ccaaatacag tgaatggtat tataacttct ggagcattaa cacgttataa taataaacta
      541 tttggcaatc ctgatagaaa catatcacct gatacttata tagaaaacaa tttaagtgag
      601 ggggtatgtt ctgatttaga ggtaatggaa aaatataaac ttgatgattt gaatgcgaaa
      661 caaatctcta tggggctcgt cttttcaata atggatggtg ttaggtattt gaaagacaat
      721 gctcaacaat ttacagataa tattttgata ttgcatggca aggaagatgg gctagtaagc
      781 tatgtagatt ctttacagct ttatcaagaa ataggatcag cacataaatc attacacatc
      841 tatgatcgtt tggagcatga aatatttaat gaaagttctt ataatagaac tatttttaac
      901 gaagttattg aatggcttga aacggaatta acttataact aaaacagtat agttccgtgt
      961 atttgattat aagaaattat gaggatatta aacatactaa gattagctat gaag
//
LOCUS       DANRDI010000305         1009 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.312, whole genome shotgun sequence.
ACCESSION   DANRDI010000305 DANRDI010000000
VERSION     DANRDI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.312"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>1009)
                     /locus_tag="PZ377_002880"
     rRNA            complement(<1..>1009)
                     /locus_tag="PZ377_002880"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          230 a          298 c          196 g          285 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaaagc tatttcggag
      601 agaaccagct atctccaggt tcgattggaa tttctccgct accctcagtt catccgctca
      661 cttttcaacg taagtcggtt cggtcctcca ttcagtgtta cctgaacttc aacctgacca
      721 agggtagatc acctggtttc gggtctacga ccaaatacta aacgccctat tcagactcgc
      781 tttcgctacg gctccacatt tactgcttaa ccttgcatca aatcgtaact cgccggttca
      841 ttctacaaaa ggcacgccat cacccattaa cgggctctga ctacttgtaa gcacacggtt
      901 tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac ggtactggtt
      961 cactatcggt cactagagag tatttagcct taggagatgg tcctcccag
//
LOCUS       DANRDI010000306          989 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.313, whole genome shotgun sequence.
ACCESSION   DANRDI010000306 DANRDI010000000
VERSION     DANRDI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.313"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(175..363)
                     /locus_tag="PZ377_002881"
     CDS             complement(175..363)
                     /locus_tag="PZ377_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359716.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKVMNFKKNN"
BASE COUNT          390 a          156 c          111 g          332 t
ORIGIN      
        1 tcggcataaa tgcgtcgtct attttttatt atcttcatga taatcatatt tttcttccat
       61 ctcgtcaaaa gcatcatctt tatagaaaaa caaaaagtaa gcaacaacac caataggaat
      121 aaaaaaacag tgtgaaaaca agaagaaact cgtttcaaaa tcccctcaaa tattctaatt
      181 attctttttg aaattcataa ccttaacaac caaataaata gcatttattc ccatcaaaat
      241 aacatacgga attaatatat ataaattttc caaatgagat ataaaaatta atccaagtaa
      301 aaagaaaaca tttaaaatag ttgctataac gtaaccttca tctaacactg tgtttttatt
      361 caaaataaac actccttttt taaaaaactt tatcacaaga cttactcaaa ttataaatac
      421 ctttattttg agttttaagg gggtatttta aaggtttagg gggtatcata taggttttta
      481 acttaaaaat atatacaagc tcttaaaacg caaatatgag ccaaataaat atatggtaat
      541 ttcacaaaca aaaatttcag caaaaccagt gactaatttt ttaacactgc ccatttacat
      601 gcaaattaaa aattagcata aaatccttta aaacttccac atgtttttct aaattcctgc
      661 agggcgtttt ttatcaaaaa tcgagaattt ttaatttttc tctaaaaact ccgtaagagc
      721 ataaggagaa tttgctatca ttcaaaaatg ataggtctgg caaagccaga aattccaggg
      781 gttttaaggt attttaaagt gatatcatac aaagctgtac tgtcttattt ttgtgacaca
      841 atatattgcg tttgtcttat ttttgtgaca ctacatacag catttgtcac aaaaataaga
      901 cagtactttt ttataaaaaa tcaacaaaaa agacattagt caacactaat gtcttaaata
      961 ataattttgc ttttcgaaat tccaaattg
//
LOCUS       DANRDI010000307          978 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.314, whole genome shotgun sequence.
ACCESSION   DANRDI010000307 DANRDI010000000
VERSION     DANRDI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.314"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..969
                     /locus_tag="PZ377_002882"
     CDS             <1..969
                     /locus_tag="PZ377_002882"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDM6359717.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          350 a          173 c          198 g          257 t
ORIGIN      
        1 ggtaaaaaag caggcgagtt ctatacacca caacaagtat ctaagatact ggcgaagatt
       61 gtcacagacg gtaaagataa attacgtcac gtgtatgacc caacatgtgg ttcaggttca
      121 ctgttgttac gtgttggtaa agaaacacaa gtgtatcgtt atttcggtca agaacgtaac
      181 aatactacat acaacttagc acgcatgaat atgttattac atgatgtgcg ttatgagaac
      241 ttcgatatcc gtaatgatga cacattggaa aacccagcct ttttaggcaa tacatttgat
      301 gcggttattg cgaacccacc gtatagtgcg aaatggactg cagattcaaa gtttgaaaat
      361 gacgaacgat tcagtggtta cggcaaactt gcgcctaagt ctaaagcaga ctttgccttt
      421 attcaacaca tggtacatta cctagacgat gaaggtacca tggccgttgt actcccacat
      481 ggtgtattat tccgaggtgc tgcagaaggt gtcattcgtc gttatttaat tgaagaaaag
      541 aactacttag aagctgtgat tggtttgcca gcgaatattt tctatgggac aagtattcca
      601 acatgtattt tagtatttaa aaaatgtcgc caacaagacg acaacgtact atttatcgat
      661 gcatccaatg attttgaaaa aggaaaaaat caaaatcatt taagcgatgc ccaagtcgaa
      721 cgtattatag acacatataa gcgtaaggaa acaattgata aatatagcta cagcgcgaca
      781 ctacaagaga ttgccgataa cgattacaac ctaaatatac cgagatatgt cgatacattc
      841 gaagaagaag caccgattga tttagatcaa gtccaacaag atttgaaaaa tatcgataaa
      901 gaaatcgcag aaattgagca agaaatcaat gcatacctga aagaacttgg ggtgttgaaa
      961 gatgagtaat acacaaaa
//
LOCUS       DANRDI010000308          953 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.315, whole genome shotgun sequence.
ACCESSION   DANRDI010000308 DANRDI010000000
VERSION     DANRDI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.315"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..873
                     /locus_tag="PZ377_002883"
     CDS             <1..873
                     /locus_tag="PZ377_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HDM6359718.1"
                     /translation="FKNNTLETFDATDPEVDYITEIGDYLLKYDVNSINDVRIEVIDN
                     LGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGYFNREFFDLLNDHLNEIEIRYNTLT
                     PTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIKPINKNFIVKGNGIELADLYNIKNK
                     ELFTIKRGINTSLSLYSLEQNIIAINALKYPESYNFEELIEAIPDNSENIFNDIQRST
                     NFSIVWILPISSIDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVEWSLYLKDQRI
                     NPIIYMETPTEDRN"
BASE COUNT          402 a          112 c          143 g          296 t
ORIGIN      
        1 tttaaaaata atacattaga gacatttgat gcgactgatc ctgaagttga ttacatcact
       61 gaaataggag attacttatt aaaatatgac gttaattcta taaatgatgt cagaattgaa
      121 gtgatagata atttggggca ttcaaataat atgctactaa aaacgatact acatgcagaa
      181 gttgaaatgg aagatggaaa gaaatattta ttacaaaatg ggaaatgggg ttattttaat
      241 agagaatttt ttgacctttt gaatgatcat ttgaatgaga tagaaattag gtataacaca
      301 ctaactccca caggtttagt atttaaagaa ggagaagaag gatatataaa agaaatagta
      361 ggaagattgc cagaagaata tttaatgtta cataaaaaat tcataaaacc aataaataag
      421 aattttatag taaaaggaaa tggaatagag ttggcagact tatacaatat taaaaacaaa
      481 gagcttttca cgattaaaag aggaattaat acatctttat ctctttatag tctagaacag
      541 aatataatag caattaacgc cttaaaatat ccagaatcat ataattttga agaattaata
      601 gaagctattc ctgataactc ggaaaatata tttaatgata tacaacggag tacaaatttt
      661 agtatagtat ggattttacc gatatcatct attgataata tgcccataaa agatatggtt
      721 catactagta acgtaataaa taaaaacttt caattaacta atttaggttc agtattactt
      781 aaaaataaat tagtagagtg gtctctctat ttaaaagatc aaagaattaa tccaattatt
      841 tatatggaaa cgccaactga agatagaaat taaactttta ttttaaatca ccctagttca
      901 tacctaaaag actttcacac aaacaaagga ggaaacttaa attcctcctt tcc
//
LOCUS       DANRDI010000309          949 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.316, whole genome shotgun sequence.
ACCESSION   DANRDI010000309 DANRDI010000000
VERSION     DANRDI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.316"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..300)
                     /locus_tag="PZ377_002884"
     CDS             complement(<1..300)
                     /locus_tag="PZ377_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDM6359719.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINK"
     gene            413..>949
                     /locus_tag="PZ377_002885"
     CDS             413..>949
                     /locus_tag="PZ377_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDM6359720.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGF"
BASE COUNT          292 a          153 c          126 g          378 t
ORIGIN      
        1 tttattaatt aattgcaccg tatactgact tctttgttga gaggaccata acatcatttc
       61 agaccactta tttgtaatat gtgcttcatt caaatagtca ttaacatatg ttccactacc
      121 tactttagta tagataaatc cttcagcttc taataactca atacttttaa taatcgttac
      181 tctatttacg ttgtactgta tcgccaactg cctatgtgaa ggtattttca ttccataaaa
      241 ccaatcccct gtgattattt tatctcttat gtcactagca atatctttat atttagccat
      301 cttctccctc ccttttaaaa ttggataggt aaatccattc aaaattggtt gtactcattt
      361 taaatcatat tttttaatat agaaacacat aattaaagga gtgatgatat taatgaataa
      421 acttcaagac actacttttc tatcatattt atttactatc atattgtggg gatctgcatt
      481 tccaatgata aagattgcgt taaatgattt tagtgcagaa tcattgtcgg catttcgttt
      541 aattttagca acaataattc tcttgccgtt cgtaattata aagaaattgc ctacccctga
      601 actaagagat atccctgtta tttttatttt aggattttgt ggatttgtga tatatcacac
      661 agctttaaat tttggtgaag ctttgattag tgcaggtata tctggaattc tagtctctac
      721 aacgcctatt ttttctagtg ctttagctta tatattttta aaagagcatt tttcaaaatg
      781 gaattggtta agttcactcg tcgcatttat aggaatatcc attatttcaa taagtaaaga
      841 tgattacaca actattaatg tattaggtgt ttttattatt ttacttgcat cttttagtga
      901 aagtttgtat tttactttcc agaaaaaata catagaaaaa tacggcttc
//
LOCUS       DANRDI010000310          941 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.317, whole genome shotgun sequence.
ACCESSION   DANRDI010000310 DANRDI010000000
VERSION     DANRDI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.317"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..818
                     /locus_tag="PZ377_002886"
     CDS             <1..818
                     /locus_tag="PZ377_002886"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDM6359721.1"
                     /translation="DPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVKAGLHKDTL
                     VDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIGGFIAGVIV
                     CKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLHLPNFIIEN
                     MYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGRFFIEGLRT
                     DSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTKKVK"
     gene            826..>941
                     /locus_tag="PZ377_002887"
     CDS             826..>941
                     /locus_tag="PZ377_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDM6359722.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSK"
BASE COUNT          302 a          126 c          177 g          336 t
ORIGIN      
        1 tagatcctgt ggcatttaac ttaggaccac tgagtgtacg atggtatgga attatcattg
       61 ctgtcggaat attacttggt tactttgttg cacaacgtgc actagttaaa gcaggattac
      121 ataaagatac tttagtagat attatttttt atagtgcact atttggattt atcgcggcac
      181 gaatctattt tgtgattttc caatggccat attacgcgga aaatccaagt gaaattatta
      241 aaatatggca tggtggaata gcaatacatg gtggtttaat aggtggcttt attgctggtg
      301 ttattgtatg taaagtgaaa aatttaaacc catttcaaat tggtgatatc gttgcgccaa
      361 gtataatttt agcgcaagga attggacgct ggggtaactt tatgaatcac gaggcacatg
      421 gtggatcggt gtcacgcgct tttttagaac aattacattt gcctaatttt ataatagaaa
      481 atatgtatat taacggccaa tattatcatc caacattctt atatgaatcc atttgggatg
      541 tcgctggatt tattatctta gttaatattc gtaaacattt aaaattagga gaaacattct
      601 ttttatattt aacttggtat tcaattggtc gattctttat agaaggatta cgtacagata
      661 gcttaatgct cacaagtaat attagagttg cacaattagt atcaattctt ttaattttaa
      721 taagtataag tttaattgta tatagaagga ttaagtataa tccaccgttg tatagcaaag
      781 ttggggcgct tccatggcca acaaaaaaag tgaagtagtg atagtttgag gaaattttta
      841 tcaaaaacac atcatcatac aaacccttta tggcgtgtat accgtcttgt taaattttcg
      901 aaagttttta agaatgtaat tatcattgaa ttttcgaaat t
//
LOCUS       DANRDI010000311          932 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.318, whole genome shotgun sequence.
ACCESSION   DANRDI010000311 DANRDI010000000
VERSION     DANRDI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.318"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(125..>932)
                     /gene="icaC"
                     /locus_tag="PZ377_002888"
     CDS             complement(125..>932)
                     /gene="icaC"
                     /locus_tag="PZ377_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDM6359723.1"
                     /translation="YILIPYILMGLFYSYSESLLTDSSFNKQFIENVLLGQWYGYFIV
                     VIMQFFILSYIIFKINYNLFNSKILLLLSFILQQSFLYYFTNNTAFHDTVLHYYPLSE
                     NTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVIMIVLAVATYFVFIALANGDYWNVT
                     SFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQMISAFSFFIYLLHPIILDSLFAYTN
                     IFEDNTMVFLAISLLFILGLCIGVGMILREFYIFRFIIGKQPYKLNINAY"
BASE COUNT          387 a          128 c          127 g          290 t
ORIGIN      
        1 ggcatttacg aagtttaaat gtgcaattag aatatatgta taacaatatt aaacacgcgg
       61 taaaacgaag tcagtcaatt caaactgatt tcgcccaccg cgtgttttta acatagctta
      121 ataattaata agcattaatg ttcaatttat atggttgttt tccaataata aacctaaaga
      181 tatagaattc acgcaatatc atgccgacac ctatacataa tcctaaaatg aatagtagtg
      241 atatcgctag aaagaccatt gtattatcct caaatatatt tgtatatgca aacaatgagt
      301 ctagaatgat tggatgtaat aaataaataa agaatgagaa agcactaatc atttgaatcg
      361 tattaaataa cattgtttta aaatgcgtgc aaatacccaa gataacaata aacataatac
      421 tattatatgg tgttaatgaa tatgaaaagc tggtaacgtt ccaatagtct ccatttgcta
      481 acgcaataaa cacaaaataa gtagctacag ctaatacaat cataataact aaataacgtt
      541 ctaagaaatt taatacacgt tcgtagttat aacccatata tgcacctaag aagaaataaa
      601 aaatccatcc gaatattata gtattttcac ttaatggata atagtgtagc acggtatcgt
      661 gaaacgctgt gttgttcgta aagtaatata aaaatgattg ctgtaaaata aaagataaca
      721 ataataatat tttactgttg aataggttat agttaatttt aaaaatgata taactcaaaa
      781 taaagaattg catgataaca acgataaaat agccatacca ttgacctaat aggacatttt
      841 caatgaattg tttattgaaa cttgaatctg ttaataatga ttcactataa ctgtaaaaca
      901 atcccattaa tatgtaagga ataagtatat at
//
LOCUS       DANRDI010000312          927 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.319, whole genome shotgun sequence.
ACCESSION   DANRDI010000312 DANRDI010000000
VERSION     DANRDI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.319"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            178..435
                     /locus_tag="PZ377_002889"
     CDS             178..435
                     /locus_tag="PZ377_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDM6359724.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            435..701
                     /locus_tag="PZ377_002890"
     CDS             435..701
                     /locus_tag="PZ377_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDM6359725.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
BASE COUNT          378 a          102 c          164 g          283 t
ORIGIN      
        1 gtgtgttcca ctgctgattt ctttttgagc attataggcg gtatgttgga agaaaagaac
       61 aaaagtttgt gaacgatagc atataggtat taagaatacg atgaactcgt tggtaatatt
      121 gacgtactca agtgcgtacg ctataattag cgtaacagaa aacaaaggag ggtttatatg
      181 attatcacta gccctacaga agcgagaaaa gatttttatc aattactaaa aaatgttaat
      241 aataatcacg aaccaattta tattagtggc aataatgccg aaaataatgc tgtgattata
      301 ggtttagaag attggaaaag tatacaagag acaatatatc ttgaatctac tggtacaatg
      361 gacaaagtaa gagaaagaga aaaagataat agtggtacaa caaatataga tgatattgat
      421 tgggataatc tttaatgagc aattacacgg ttaagattaa aaattcagcg aaatcagatt
      481 taaagaaaat aaaacattct tatttaaaga agtcattttt agaaattgtt gagactttaa
      541 aaaatgatcc gtataaaata acacaatctt ttgaaaaatt agagcctaaa tatttagagc
      601 gatattcaag aagaattaac catcagcaca gggtcgtcta taccgtagat gatcgaaata
      661 aagaagtatt aatactatcg gcatggtcac attatgatta atgaatattc aatatctgaa
      721 taactttaat gataagttaa ttaaagaaac tagtatttaa gtgtagggaa aatagcgacg
      781 ttaatgcgtt gttatctcta cactttttaa ttttataata gcgcaagact aaacagattg
      841 aaattagtaa caataaaaga ataacgtatt ataataagga attttaaatt gtgacttttt
      901 cggaatatta aattttagaa atatgag
//
LOCUS       DANRDI010000313          885 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.320, whole genome shotgun sequence.
ACCESSION   DANRDI010000313 DANRDI010000000
VERSION     DANRDI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.320"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            70..765
                     /locus_tag="PZ377_002891"
     CDS             70..765
                     /locus_tag="PZ377_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HDM6359726.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
BASE COUNT          375 a           86 c          174 g          250 t
ORIGIN      
        1 acgtaattga aataatcata taaaaatata ttaagacaaa atttataaat agattgggag
       61 aatagtacta tgaaattaaa aacgttagct aaagcaacat tagtattggg attgttagct
      121 actggtgtaa taacaacaga aagtcaaaca gtaaaagcgg cagaatcaac tcaaggtcaa
      181 cacaattata aatcgttaaa atactactat agcaagccaa gtatagagtt aaaaaatctt
      241 gatggtttgt atagacagaa agtgacagat aaaggagtat atgtttggaa ggatcgaaaa
      301 gattattttg ttggcttgct tggtaaagat attgaaaaat accctcaagg tgagcatgat
      361 aagcaagatg catttttagt catcgaggag gaaactgtta atggaagaca atattcaatt
      421 ggtggtttaa gtaagacaaa tagtaaagaa tttagtaaag aagtcgatgt taaagtaaca
      481 agaaaaattg atgaatcatc ggaaaagtct aaagatagta aatttaaaat tactaaagaa
      541 gaaatctcgt taaaagagtt ggactttaaa ttaagaaaaa aattgatgga agaagaaaaa
      601 ttatatggtg ctgttaataa tagaaaaggt aaaattgtag ttaaaatgga agatgataag
      661 ttttatactt tcgaacttac aaaaaaacta caaccgcatc gcatgggtga cacgatagat
      721 ggtaccaaaa tcaaagaaat taatgttgag ctagaatata aataatcttt ggacaagcag
      781 actagtaatt gtagggaagt taagcgataa catattgctt agcttctttt ttattttgtt
      841 atgatgaaaa aaggagcggg tttatgatca agtttttgga aaaac
//
LOCUS       DANRDI010000314          869 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.321, whole genome shotgun sequence.
ACCESSION   DANRDI010000314 DANRDI010000000
VERSION     DANRDI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.321"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            21..725
                     /locus_tag="PZ377_002892"
     CDS             21..725
                     /locus_tag="PZ377_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDM6359727.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
BASE COUNT          356 a          103 c          170 g          240 t
ORIGIN      
        1 tacgaatgga gcatacaact atgaaaatga cagcaattgc gaaagcaagt ttagcattag
       61 gtattttagc aacaggaaca ataacgtcat tgcatcaaac tgtaaatgcg agtgaacata
      121 aagcaaaata tgaaaatgtg acaaaagata tctttgactt aagagattac tatagtggcg
      181 caagtaagga acttaaaaat gttactggtt atcgttatag caaaggtggc aagcattacc
      241 ttatctttga taaaaataga aaattcacaa gagtacagat atttggtaaa gatattgaaa
      301 gatttaaagc acgtaaaaat ccgggattag acatatttgt tgttaaagaa gcggaaaacc
      361 gtaatggcac agtgttttca tatggtggtg tcactaagaa aaatcaagac gcttattatg
      421 attatataaa cgcaccaaga tttcaaatca agagagatga aggtgacggt attgctacgt
      481 acggtagagt acactacatt tataaagaag agatttcact taaagaactc gactttaaat
      541 tgagacagta tttaattcaa aattttgatc tgtataaaaa gtttcctaaa gatagtaaga
      601 taaaagtgat aatgaaagat ggcggctatt atacgtttga acttaataaa aaattacaaa
      661 caaatcgcat gagtgacgtc attgacggta gaaatattga aaaaatagaa gccaacatta
      721 gataattcaa tgaaatatgg ataatagtaa aatatggata gtatagagga gttaggcaac
      781 ataagttgct tagcttcttt tttgtgttgg agagatgaaa atgaagcgta tcgatgaata
      841 ataaaaacac caataaaact tgtggaaat
//
LOCUS       DANRDI010000315          833 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.322, whole genome shotgun sequence.
ACCESSION   DANRDI010000315 DANRDI010000000
VERSION     DANRDI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.322"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            298..>833
                     /locus_tag="PZ377_002893"
     CDS             298..>833
                     /locus_tag="PZ377_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDM6359728.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDI"
BASE COUNT          369 a           99 c          135 g          230 t
ORIGIN      
        1 aacataaaaa aatagacaag taccgaagta cctgcctaaa taacaacaag attaacatgt
       61 gaataatgga aataaaaagt cagcccgaag gctaacttac gaatagatga aaatttgaac
      121 acattgctgt gtctaaaatg attatagcat aaataacgaa tatttccagc tcaaaattaa
      181 tatattgtaa taataatatt ttatatcttt gttaataatt atttaattga tttacataaa
      241 taataattgt aaaattactt tgtaatcgat tgcaaataaa ttataggaga aaataaaatg
      301 aataaaaaac tattaacaaa aacattgata gcaagtgctt tagttttaac aacagtaggt
      361 tcaggttttc attcttcttc aaattataat ggtattaata acgttgcaaa agctgagcaa
      421 acgacagata acgaattgtg gaaaaatgta agagacgctt taaaagaagc gaatattatc
      481 gataaaacag ataatgaaaa tgtcaaagtg acttataaat tagcaaatgg cggtgaaaac
      541 agtattgaag gtaccgccaa tttaaatgaa cttagcaatt ctaataacaa accgatcaat
      601 agcgatagtg taaaaagcat tgatttgtca aggaaaaatc ccaatggaga tagatttgac
      661 gcaaatgatg catggaaaaa attaactgat aaattaaaag aaaagcagat tgtgaaagac
      721 ggtgatacag taactattca tagtaaagac gaaaaagacc ctaaaatctc agctaaagtc
      781 ggtgaagtct ataatgataa caaacgattg atgttgttta aaaaggatat aga
//
LOCUS       DANRDI010000316          808 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.323, whole genome shotgun sequence.
ACCESSION   DANRDI010000316 DANRDI010000000
VERSION     DANRDI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.323"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>808)
                     /locus_tag="PZ377_002894"
     rRNA            complement(<1..>808)
                     /locus_tag="PZ377_002894"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          170 a          242 c          173 g          223 t
ORIGIN      
        1 aaggatgtca agatttggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggtccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac
      181 tcatcgttta cggcgtggac taccagggta tctaatcctg tttgatcccc acgctttcgc
      241 acatcagcgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc tccatatctc
      301 tgcgcatttc accgctacac atggaattcc actttcctct tctgcactca agttttccag
      361 tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa aaaaccgcct
      421 acgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct
      481 gctggcacgt agttagccgt ggctttctga ttaggtaccg tcaagatgtg cacagttact
      541 tacacatatg ttcttcccta ataacagagt tttacgatcc gaagaccttc atcactcacg
      601 cggcgttgct ccgtcaggct ttcgcccatt gcggaagatt ccctactgct gcctcccgta
      661 ggagtctgga ccgtgtctca gttccagtgt ggccgatcac cctctcaggt cggctatgca
      721 tcgttgcctt ggtaagccgt taccttacca actagctaat gcagcgcgga tccatctata
      781 agtgacagca agaccgtctt tcactttt
//
LOCUS       DANRDI010000317          807 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.324, whole genome shotgun sequence.
ACCESSION   DANRDI010000317 DANRDI010000000
VERSION     DANRDI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.324"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..662
                     /gene="splE"
                     /locus_tag="PZ377_002895"
     CDS             <1..662
                     /gene="splE"
                     /locus_tag="PZ377_002895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HDM6359729.1"
                     /translation="TGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSGTGF
                     IVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVED
                     KAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYINKFQMYESTGKVLSVKGNM
                     IITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTD
                     K"
BASE COUNT          313 a          123 c          144 g          227 t
ORIGIN      
        1 tgactggcgt cggcacaaca gtggttgagg gtattcaaca aacggctaaa gctgaacata
       61 atgtgaaact aatcaaaaat actaatgtag caccatacaa tggtgtcgtt tcgataggat
      121 ctggaacagg tttcattgtc ggtaaaaata caattgttac caacaagcat gtcgttgcag
      181 gtatggaaat tggtgcacat attatagcgc atcccaatgg tgaatataat aatggcggat
      241 tttataaagt taaaaaaatt gtccgttatt caggtcaaga agatattgcc attctacatg
      301 tggaagataa agctgttcat ccaaaaaaca ggaattttaa agattacaca ggcattttaa
      361 aaatagcatc agaagctaaa gaaaatgaac gcatttcaat tgttggctat ccagaaccat
      421 atataaataa atttcaaatg tatgagtcaa caggaaaagt gctgtcagtt aaaggcaaca
      481 tgattattac tgatgctttc gtagaaccag gcaactcagg ttcagctgta tttaacagta
      541 aatacgaagt tgtaggtgtt cactttggtg gaaacggccc tggaaataaa agtacaaaag
      601 gatatggtgt ttatttctct cctgaaatta agaaattcat tgcagataac acagataaat
      661 aaatccttac atagataaat gattttaaaa attaacaaca aactcaacaa ttcaaatcat
      721 ctctgtgatt ccatttattc gaaatgatta aaaaaaataa aacttcaaaa agctaacatt
      781 ataattatac aaatacttag aggagca
//
LOCUS       DANRDI010000318          782 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.325, whole genome shotgun sequence.
ACCESSION   DANRDI010000318 DANRDI010000000
VERSION     DANRDI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.325"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(118..660)
                     /locus_tag="PZ377_002896"
     CDS             complement(118..660)
                     /locus_tag="PZ377_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDM6359730.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
BASE COUNT          257 a          155 c           92 g          278 t
ORIGIN      
        1 gtcaactact gccaatatag tgttgtagtg tctaagacat aaaagttcat ctcagtcaca
       61 attactttat agcctatcca tcatcttttc tacatgaaat ttttcaagtg atatatttta
      121 tttctcaata ttatctaaaa aatactgttc tacatctttc cagctattta cgcgttcaaa
      181 tcgatcatca ttaatattat gcactgctgt aaacataatc ggtgtaccag taaaaatttc
      241 aagctgtcta ggattgtcat ctattaaata atcagcttta acgatgtttt ttctaccaca
      301 aaaaacaaaa tgctgaggat ctaaaaatgg aaagaactct agtaaccatt catatttatc
      361 actaaatgat gttggtacat ccattgctgc tgtagcaata tatacatcat aatgttcagt
      421 taattttttc acaacttctt gtgcatacgg catcacttta agatgtctga agaagcctga
      481 ttctctcaat acttctgtaa ttaatccatc atgttcagga ataacatgtt taagtttttg
      541 accattcaaa gcttccattt taatacctaa atccgctcta aaattgacag catcaatgat
      601 ttctcctaat gtatctgcca atacttcatc catatcaatc gcgattgatt tacgggtcat
      661 tttaatcttc tcctcaaaat atcagtattg attcagtata gcaaaaaagc aattgataca
      721 aaacaatatc atctattgtt gctttttaca ctgaatactc tcacctcatc attcacactg
      781 ca
//
LOCUS       DANRDI010000319          768 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.326, whole genome shotgun sequence.
ACCESSION   DANRDI010000319 DANRDI010000000
VERSION     DANRDI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.326"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            3..467
                     /locus_tag="PZ377_002897"
     CDS             3..467
                     /locus_tag="PZ377_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDM6359731.1"
                     /translation="MYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFYNYTEPSSDE
                     LFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEFDFTKEDKLK
                     VSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKEQEE"
     gene            677..>768
                     /locus_tag="PZ377_002898"
     CDS             677..>768
                     /locus_tag="PZ377_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDM6359732.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCL"
BASE COUNT          307 a           77 c          139 g          245 t
ORIGIN      
        1 aaatgtatag cgagattgcg aataagatta ttagcatgat accggtagag tgggaaaaag
       61 tatatgcaat ggcatatatc gatgaagaat gtggagaagt gttttacaat tatactgaac
      121 caagcagtga tgaattattt tactatacaa gtgtgataaa gaagtataat ttattgaaat
      181 caagctttat ggattcagta tatgagttgc atgatcaatt tgaggaacta agagaagtat
      241 ttatagaaga aggtcttgaa ccatggacat catgcgaatt tgattttaca aaagaagata
      301 aattaaaagt ttcatttgat tatattgatt ggataaatac agagtttgat caattaggcc
      361 gtgaaaatta ttatatgtac aaaaaatttg gtgttttacc agaaacggaa tatgaaatgg
      421 aggaggttaa agaaatcgag caatatatta aagagcaaga agaatagaca atcttaatgt
      481 aagattaaac aataaagctt tgtttagtct ttttagcgtt taagtaaaaa acaatagaga
      541 ccataaagtt gtcatcaaat aataatataa agataatttt aggcttttat acttttaatc
      601 gaaaagaatc taagtactta taactttaaa tacttaatat gctattacaa aatcaatgta
      661 taggagatag gtaaatatga ctactaagga aaatatagac attcttcgaa agccaggtgc
      721 acaagcctta agtttagcat cattatttat gatacttttt tcatgtct
//
LOCUS       DANRDI010000320          760 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.327, whole genome shotgun sequence.
ACCESSION   DANRDI010000320 DANRDI010000000
VERSION     DANRDI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.327"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            214..639
                     /locus_tag="PZ377_002899"
     CDS             214..639
                     /locus_tag="PZ377_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDM6359733.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
BASE COUNT          294 a          116 c          113 g          237 t
ORIGIN      
        1 ctatatgctt ttcatgatta ttatatacaa ctaatcaatc cctataaact aaaaacattg
       61 cttaccactt ttcaaaatgc acgagtcata tccctcgtta aaaactatta ctaaaaatta
      121 aaatataaaa aatgcaaaaa gtatgtttta aactttatga aacgtgttat acaaatagtg
      181 taatatcata caacccgaaa aggagattga gatatgaaac taaaatcatt tgttactgcc
      241 actttagcat tgggattatt atcaacggtc ggagctgcat taccgagtca cgaagcatct
      301 gcagatagta ataacggcta taaagaaatg actgtggatg gttatcacac tgttccttac
      361 acaatttcag tagatggtat tactgcatta catcgaactt actttatctt cccagaaaat
      421 aaaaatgttc tttatcaaga aattgacagt aaagtaaaaa atgaattagc ttctcaacgt
      481 ggtgttacaa cagaaaaaat taataatgcc caaacagcaa cttatacgct tactttgaat
      541 gatggtaata aaaaagtagt gaatctaaag aaaaatgacg acgctaaaaa ttcaattgat
      601 ccaagtacaa tcaaacagat acaaattgta gttaaataat aatttgaaaa tagttttata
      661 gggcaagcgc tgaaaaattg gcgtttgtct ttttagttat tgaacttgta aattattgtt
      721 gtttgcttct cgtagtaaca gattggccta tacaatagta
//
LOCUS       DANRDI010000321          750 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.328, whole genome shotgun sequence.
ACCESSION   DANRDI010000321 DANRDI010000000
VERSION     DANRDI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.328"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..382
                     /locus_tag="PZ377_002900"
     CDS             <1..382
                     /locus_tag="PZ377_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDM6359734.1"
                     /translation="QYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELYVLGIL
                     NLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIKREKFS
                     KVTMLIQACYNYLEKGIQVNLNNK"
     gene            446..>750
                     /locus_tag="PZ377_002901"
     CDS             446..>750
                     /locus_tag="PZ377_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDM6359735.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     "
BASE COUNT          274 a           70 c          145 g          261 t
ORIGIN      
        1 tcaatataca tttgatttta gttgtgacta tgtatcgtat ttattgtcgt cacaagaatt
       61 attgcggaat ctaaagtgtt atttaaatat gtgtcaatta tcattagaag agttatacgt
      121 tttaggaata ttaaatctgc ataaaggtca attgacggtt aaagaattac agggtgaatt
      181 ccatcacccg atatttgcgg ttagcccgat tttaaagtgt ttaattttga agggattagt
      241 taaaaaagag agatgtgaat tagatgaaag gcgtgttatt gtaacaatca agcgagaaaa
      301 gttttctaaa gtcactatgc ttattcaagc atgttataat tatcttgaaa agggaataca
      361 agttaatctt aataataaat agttaatcac ttgtatttca tatacctatg aagaatagtt
      421 tatagtttta agtgggaggg atattatgat tttagataat gtgaatccaa atgatttatt
      481 tccaactgaa aaaaagggtc cgtcagtgct aggaataatt gagtatcaag ttcaaggtga
      541 gaatgaattt gaaggggcat ttatagcaac aaatgaaaga ttaattatga atgtagatat
      601 gaatggccaa ttttattata gaagtattag ttataatgaa atagagaaga tagattatga
      661 tggtcaaact ataatgttca aatttaatat tggtaatgtc ccaatgcatg atattaaaag
      721 tgctaacgtt gaaatgtttg ttgaatatgt
//
LOCUS       DANRDI010000322          739 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.329, whole genome shotgun sequence.
ACCESSION   DANRDI010000322 DANRDI010000000
VERSION     DANRDI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.329"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..407
                     /locus_tag="PZ377_002902"
     CDS             <1..407
                     /locus_tag="PZ377_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1453 family protein"
                     /protein_id="HDM6359736.1"
                     /translation="RMKAQNYPVNEKKIVLPPFFMATGALMYVVPYFRLTGSEMLEAF
                     IIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILISLLIIRTVMKIFISNEIDPGEL
                     GGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            531..>739
                     /locus_tag="PZ377_002903"
     CDS             531..>739
                     /locus_tag="PZ377_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDM6359737.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGK"
BASE COUNT          266 a           98 c          134 g          241 t
ORIGIN      
        1 ttcgtatgaa agctcaaaat tatccggtaa atgagaaaaa aatagttttg ccaccgtttt
       61 ttatggcgac cggtgcattg atgtacgtcg ttccatattt taggctaaca ggatcggaaa
      121 tgctagaagc ctttataatt ggtttgcttt tttctacagt tctaatttgg acttctcgat
      181 ttgaagtcaa aggtacagaa atttatatga aacgatctaa agcatttcca gttattttga
      241 tttcattact tatcattcgt actgtgatga aaatattcat tagtaatgaa atagatcctg
      301 gagaattagg cggcatgttc tttttattag cattctgtat gattgttcct tggagagcag
      361 caatgctata taaatacaaa aaactaaaga aaacattaat caattaatta cttttaaaac
      421 cacttgtgat cgacttctaa atcagtcaat gagtggtttt aattttactt gaaaaggtga
      481 aatgattata ttaaaataaa agcgaacaaa tgttctatat ggaggcagta atgaaaatta
      541 tacatacagc agactggcac ttagggaaaa tattaaatgg caaacagctt ttagaagatc
      601 aagcgtatat tttagatatg ttcgtagaaa aaatgaaaga agaagaacct gatatcattg
      661 tgatagctgg agatttatat gacacaacat atccaagtaa agatgcaatc atgttattag
      721 aacaagcgat tggaaagtt
//
LOCUS       DANRDI010000323          730 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.330, whole genome shotgun sequence.
ACCESSION   DANRDI010000323 DANRDI010000000
VERSION     DANRDI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.330"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..193
                     /locus_tag="PZ377_002904"
     CDS             <1..193
                     /locus_tag="PZ377_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359738.1"
                     /translation="KYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKGP
                     FAPDFIKVWKERYKKIRQY"
     gene            484..>730
                     /locus_tag="PZ377_002905"
     CDS             484..>730
                     /locus_tag="PZ377_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HDM6359739.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQ"
BASE COUNT          275 a           92 c          125 g          238 t
ORIGIN      
        1 aaagtatcat ttattagatg aagatgaata tgagcaacat atgaatcaaa tgaattatcc
       61 gcatgatatt gatattattt taagacgcaa cgtggacatt cttcaacaat ggatagaaca
      121 gaagaagggc ccattcgcgc cagattttat caaagtttgg aaagaacgtt acaaaaaaat
      181 tagacagtat taagcaaata aagtgtaaat actgattaaa tcatttatat tgacttagtg
      241 atgtaaagaa gtatgataac ttttgctgtc gtgtggggac acagtatcca tagattaaat
      301 tgaatagaat ataacatacg aataagacaa cgtatttaat cacatgaata tgacagaggt
      361 tgagatatct taattttaaa aatgtaaatt gtaagaagaa tttacatcta caaattatgt
      421 atccaacctc tattcatttt tatgcttatg aataggatag ggcaatagat taggggggat
      481 tgtatgatta aacgatattt gcaatttgtt aagccataca aatatcgtat ttttgcaacg
      541 attattgttg ggataattaa gtttggtata ccaatgctta taccactatt aattaaatat
      601 gcaatagatg gcgtgattaa taaccacgca ctaacgactg atgaaaaagt tcatcattta
      661 actattgcca ttggtatcgc attatttatt tttgtaatag tgagaccacc aattgaattt
      721 atacgtcaat
//
LOCUS       DANRDI010000324          717 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.331, whole genome shotgun sequence.
ACCESSION   DANRDI010000324 DANRDI010000000
VERSION     DANRDI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.331"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..503
                     /gene="lpl1"
                     /locus_tag="PZ377_002906"
     CDS             <1..503
                     /gene="lpl1"
                     /locus_tag="PZ377_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HDM6359740.1"
                     /translation="DDEGMVLNLDRNTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNN
                     KIILLDEVKDDKLKQKIENFKFFGQYANLKELRKYNNGDVSINENVPSYDVEYKMSNK
                     DEIVKELRSRYNISTEKSPILKMHIDGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLS
                     YKPAKK"
     gene            534..>717
                     /locus_tag="PZ377_002907"
     CDS             534..>717
                     /locus_tag="PZ377_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDM6359741.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDL"
BASE COUNT          298 a           69 c          136 g          214 t
ORIGIN      
        1 tggatgatga aggtatggtt ttaaatctgg ataggaatac tagaaccgct aaaggccatt
       61 attttgttac tacattttac cggaatggta aactaccaga tgaaaagaat tataaaattg
      121 aaatgaaaaa taataagatt attttattag atgaagtaaa agatgataag ctcaagcaga
      181 aaatagaaaa ctttaaattt ttcggtcaat atgcaaatct taaagaattg agaaaatata
      241 ataatggtga tgtttcaatt aatgaaaatg ttcctagtta tgatgttgaa tacaaaatga
      301 gcaataaaga tgaaatagtt aaggaattaa gaagtcgtta taatatttcg actgaaaaat
      361 cacctatatt aaaaatgcat attgatgggg acctgaaagg cagttctgta ggttatagaa
      421 agttagaaat tgacttttca aaacgtgaaa acagcaaatt atcagtcatt gaatttttaa
      481 gttataaacc agcgaaaaaa tagtattgat aaggaatatt agggtgtgaa aaaatgcgat
      541 atctaaaaaa gcttgcatgg ttcataagtg ttattatttt gggcattttt ataataggtt
      601 gtgatagttc aagcgatact ggggaaaaag caaaagaaga ttcaaaggaa gaacaaatca
      661 aaaagagctt tgcgaaaaca ttagatatgt atccaattaa gaatctcgag gacttat
//
LOCUS       DANRDI010000325          702 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.332, whole genome shotgun sequence.
ACCESSION   DANRDI010000325 DANRDI010000000
VERSION     DANRDI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.332"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(10..159)
                     /locus_tag="PZ377_002908"
     CDS             complement(10..159)
                     /locus_tag="PZ377_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359742.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
BASE COUNT          269 a          111 c           84 g          238 t
ORIGIN      
        1 cgatgttcct tatttatcct cataaaaaac aatagctgaa atatataacc aattactgtg
       61 tgtttgaatt tctaatacat gaatatcatt ctttaaaaac ctattaactt gtttatctaa
      121 ttgacgatga gtaaaagtag cgaaagtttt aatcttcatg atatctctcc ttttataaaa
      181 atagcaatcc ttattacaat attaaacact tttattttgg attttaagag gcattttgaa
      241 gacttagagg caaatcatat aggttttgta cttaaaaatc tatacagtct cttaaaacgc
      301 aaatatgagc caaataaata tatgctaatt tcacaaacaa aaatttcagc aaaatcagtg
      361 actaattttt tagatactgc ccatttacat gcaaatttaa aaattagcat aaaatacttt
      421 aaaacttccc catgtttttc taaattcctg cagggctttt cttatcaaaa acttagaaat
      481 tttaatttaa ctctaaaatc tccgtaagag cataagggaa atttgctatc attcaaaaat
      541 gatagatctg gcaaggccag acattccaag ggttttaagt tattttaaag tgatatcata
      601 caaagctgta gtgtcacact tttgtgacac tacatacagc atttgtcaca aaaataagac
      661 agcacttttt tacagaaaaa caataaaaaa agacatcagt ta
//
LOCUS       DANRDI010000326          682 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.333, whole genome shotgun sequence.
ACCESSION   DANRDI010000326 DANRDI010000000
VERSION     DANRDI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.333"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(238..669)
                     /locus_tag="PZ377_002909"
     CDS             complement(238..669)
                     /locus_tag="PZ377_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDM6359743.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDTILEKITSGDIN
                     NGEFDLVIMSNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
BASE COUNT          225 a          108 c           76 g          273 t
ORIGIN      
        1 attcaacttt gcaacagaac ctatattttg ctataattat taaattaatt gcctttgtgt
       61 tagtattccc tggattacta acactttggt tagctgttct aagtgataca ggtgcagcaa
      121 tattagttat attaaattca ttacgtttat taaaaaaata aaaaagccta aatactctct
      181 cgatttgtga gaaagtattt aggctttttg aattaataaa tttcttactt cttatgttta
      241 tttaacaatt agtactggga cagaacttct tttagcaatc ttatgtgtaa catctcctaa
      301 aacatgttta atattcaaat cgactctatg attactcata attactaaat caaattcacc
      361 attatttata tctccagaag taattttttc taagattgta tctttaatat gcccaaaatc
      421 tacatctatt ttatattgaa tatctctttt ttctaattca cgtaaaaatg gagtgagttt
      481 tttctctttt tctctaataa tatcttcttt atgcttatta taatacttga cactaattgc
      541 aagatcattt tctgttacaa cgtgataaat tacaacaact gaatcttcat cggtgacttt
      601 ttctaaatag tcagggatag cactaaaatc attttcgaaa tcatagggta ataatatatt
      661 tttgaacatc tacaccagcc tc
//
LOCUS       DANRDI010000327          649 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.334, whole genome shotgun sequence.
ACCESSION   DANRDI010000327 DANRDI010000000
VERSION     DANRDI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.334"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..291
                     /locus_tag="PZ377_002910"
     CDS             <1..291
                     /locus_tag="PZ377_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDM6359744.1"
                     /translation="ERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKE
                     DDFVAYGSPEHNYQFGGSLIKSKKLSTLLKPVHQTKSPDEIKKELESKKNDR"
BASE COUNT          245 a           90 c           99 g          215 t
ORIGIN      
        1 gagcgtatta cactctatct taagcataat actaaagaac ccaataccat caaaactgta
       61 catttcacaa gtttaaaaag aggacctatg ggcgatgcag taattgaagg ttacataaat
      121 gaaaataaag aagatgattt tgttgcatat gggtctccag aacataatta tcaatttggt
      181 ggaagtttaa tcaaaagtaa aaaattaagc acgttattaa aaccagtaca tcaaaccaaa
      241 tcacctgatg agataaaaaa ggaattagag tctaaaaaga acgaccgcta aaactatgcg
      301 gccgttttaa ttttgttttc aagttaatca tacttacata taaaaatgaa aagttgaatt
      361 tttagtacaa tttccactgt atttatctgt taaccacttt atacccgcaa tatttcgttg
      421 atcttatata aacacttttg gctgcttttc cttgacctag attaaaatta tagattttct
      481 ttgtaagagt gtgttttgta gtatcataaa tatgtaaact gtataaaatt ccaccacatt
      541 ttaggagctg gtgatagatg tcgaaaaaga aaattttaat attaactagt attatgttaa
      601 tcatattaat tagtgttgca ggtatctatt taaaagtgaa atatgacga
//
LOCUS       DANRDI010000328          646 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.335, whole genome shotgun sequence.
ACCESSION   DANRDI010000328 DANRDI010000000
VERSION     DANRDI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.335"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            335..>646
                     /locus_tag="PZ377_002911"
     CDS             335..>646
                     /locus_tag="PZ377_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDM6359745.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EV"
BASE COUNT          237 a           91 c           93 g          225 t
ORIGIN      
        1 agaagctgtg actatgataa agtactacta catagttaat cattagtggt tctttatcat
       61 tttcgcctcc cttttcttat tgttttgata cacaaaaatt taagttcaaa ctgtcgaata
      121 aagttatatt tgatttcaaa ttatccctaa attattaatt ttacaattgt ggcagatttt
      181 caaaataata attatttcct cattatttat aaatttatat ttaaatttca ttctttatag
      241 ggtaagatta ggactatagt atgatgtgta aataatataa attaaggtat agtaaagcta
      301 actcagaaat gacttatcat tcggaggtta cattatgaat aaactattac agtcattatc
      361 agccctcggt gtttctgcta cactagtaac accaaattta aatgcagatg caacgacgaa
      421 tactacacca caaattaaag gcgctaatga tatcgttatt aagaaaggtc aagattataa
      481 ccttctaaac ggcataagtg catttgataa agaagatgga gatttaaccg ataaaattaa
      541 agtcgatggc caaattgata catctaaatc tggtaaatat caaattaaat atcatgtcac
      601 tgattcagat ggtgcaatta aaatttccac taggtatatt gaggtt
//
LOCUS       DANRDI010000329          642 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.336, whole genome shotgun sequence.
ACCESSION   DANRDI010000329 DANRDI010000000
VERSION     DANRDI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.336"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..360
                     /locus_tag="PZ377_002912"
     CDS             <1..360
                     /locus_tag="PZ377_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDM6359746.1"
                     /translation="GNWRDKFFKEREGKYQVEGCFIESGALNNKSDEYGIKRNRHAQI
                     YYNSVRNRDKQIEISKIAKNTNINKNLIQRVYEHIFENKYLLESGFKQFDPDFYMAQS
                     WQRLREGKNIKKMDIIM"
     gene            481..>642
                     /locus_tag="PZ377_002913"
     CDS             481..>642
                     /locus_tag="PZ377_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359747.1"
                     /translation="MLILKIKEINDKSVTYKYFPNNDENIKPGIIQMDIDSLEVINAE
                     KSSLEKNTRD"
BASE COUNT          285 a           55 c          103 g          199 t
ORIGIN      
        1 ggcaattggc gagacaagtt ctttaaagag cgtgaaggta aatatcaagt agaaggttgt
       61 tttattgaat caggtgcact taataataaa agtgatgaat acggtatcaa aaggaacaga
      121 catgctcaaa tttattataa ttcagttaga aatcgagata aacagataga gatttctaaa
      181 attgctaaaa atactaatat aaataaaaat ttaattcaaa gagtttatga acatatattt
      241 gaaaataagt atttattaga aagtggattt aaacaatttg atcctgactt ttatatggct
      301 caaagttggc aacgtttaag agaagggaaa aatattaaaa aaatggatat aataatgtag
      361 aaacatgaag ctcttgaaca ctatctaatg aataagtata atttacacta tatagaggca
      421 cataaactta cagaaataaa atataattat agtattttaa ttaattagag gtggaaatga
      481 ttgctaatat taaaaataaa agaaattaat gataaaagtg ttacatataa atattttcct
      541 aataatgatg aaaatattaa gcctggtatt attcaaatgg atatagatag tcttgaagtt
      601 atcaatgctg aaaaatctag tttagaaaaa aatacaagag at
//
LOCUS       DANRDI010000330          641 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.337, whole genome shotgun sequence.
ACCESSION   DANRDI010000330 DANRDI010000000
VERSION     DANRDI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.337"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..602
                     /locus_tag="PZ377_002914"
     CDS             <1..602
                     /locus_tag="PZ377_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HDM6359748.1"
                     /translation="NGSSKYFDRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVA
                     YETTLLLDKNNSSGLIDKGNQDLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVS
                     IVMNTEHHDKLIKELAQMGLGGLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKY
                     EPIMYNKKKQALKGIYQFALNYVFDIKIIPELLRIVNIK"
BASE COUNT          241 a           84 c          119 g          197 t
ORIGIN      
        1 ttaatggcag tagtaaatac tttgatagac gtgagggaca tatctacaca gaagtatcag
       61 acagtgtagt tcatggctct gatagaacat tagatgatct acttaaagag atatttatta
      121 atgagtgtgt cgcatatgaa acaacattgt tattagataa gaataattca agtggtttaa
      181 ttgataaagg taaccaagac ttatcacttt ataatcaagg tatcaaagaa gtaagtaaca
      241 catcaatata cgatggtatc aagcaagcga tgaaagatat accacaaacg tttaggcgta
      301 atgtatctat tgtgatgaac acagaacatc atgataagct catcaaagag ttggcacaga
      361 tgggattagg tggacttgca ggtgatttaa gtaacctatt taatgtgact aatgtagtgg
      421 ttactgatga tgcacatgat gtctttgttg gagactttgg acatgccata tatgctaaat
      481 acgaacctat tatgtataac aaaaagaaac aagcattaaa aggtatttat caatttgcat
      541 tgaactatgt gtttgatata aaaataattc cagagttatt gcgcatagtt aatattaaat
      601 aaaaaaatta tttaaataaa attttatagc cccctgccca t
//
LOCUS       DANRDI010000331          637 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.338, whole genome shotgun sequence.
ACCESSION   DANRDI010000331 DANRDI010000000
VERSION     DANRDI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.338"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(44..541)
                     /locus_tag="PZ377_002915"
     CDS             complement(44..541)
                     /locus_tag="PZ377_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDM6359749.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDH
                     CASPY"
     gene            complement(543..629)
                     /locus_tag="PZ377_002916"
                     /pseudo
     CDS             complement(543..629)
                     /locus_tag="PZ377_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          202 a           94 c           84 g          257 t
ORIGIN      
        1 cgttggtctc ttcgaaacta cggcttttct ctgtgctata aaatcagtat ggcgaagcac
       61 agtgatcatt gaattctgac tttatataat caagagtatc tttggataca ctgatgatat
      121 ctataacagc attgctaatg tagaatatat tttcttgatt atagaataca ataaatggct
      181 ccactaaacc ttttttaata tataaagaat aatcaattaa gtctttgttt tttatatttt
      241 taatagttgc aataacatac ttctcatcgg tagaatggat ttcttcgtac cagttagtaa
      301 tattaaaaaa acgaccaatt cttgtttttt ctctaaattt aacaggatat aagtattcag
      361 acaaaaatgt acatttcgtc tttgattggt tattaaatat aacattaata atattagaaa
      421 tttgcttttt attgagaaca tcaatatcta agcgtatcct atttttctca ggtgcttgaa
      481 aatagggaat ttcatgttct aaatcaaaat gtatttgttg tgtttcaagg ttaataatca
      541 ttttaatctc caattctttt taggttagca tcgtatgtac cacttcttgt tttcttgcta
      601 cctaaattct taatactgct tgcacctttt catttta
//
LOCUS       DANRDI010000332          613 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.339, whole genome shotgun sequence.
ACCESSION   DANRDI010000332 DANRDI010000000
VERSION     DANRDI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.339"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            58..273
                     /locus_tag="PZ377_002917"
     CDS             58..273
                     /locus_tag="PZ377_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359750.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            298..561
                     /locus_tag="PZ377_002918"
     CDS             298..561
                     /locus_tag="PZ377_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDM6359751.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
BASE COUNT          264 a           87 c          107 g          155 t
ORIGIN      
        1 atacttatca cctccttagg ttgataacaa cattatacac gaaaggtgga acaacaaatg
       61 aacaaaaaat cagaagggtt agacataatc ataccaagga ttttcagaag agatcatgcg
      121 tcagtagaat ctttaacaga aaatgaacgt cgactaagaa aggaaatatt aggaagtatt
      181 aaaaaaggtg attacagcta cttagaaata aacaaagttt tctatgcatt agatagagaa
      241 cttcaataca gagcgaataa taacaaactt taacatttat cgaaaggagt gatagagatg
      301 ccaaaaatca tagtaccacc aacaccagaa aacacatata gaggcgaaga aaaatttgtg
      361 aaaaagttat acgcaacacc tacacaaatc catcaattgt ttggagtatg tagaagtaca
      421 gtatacaact ggttgaaata ttaccgcaaa gataatttag gtgtagaaaa tttatacatt
      481 gattattcac caacaggcac tctgattaat atttctaaat tggaagagta tttgatcaga
      541 aagcataaaa aatggtatta ggaggatatt aaatgagcga cacatataaa agctaattaa
      601 tagcagtact gtg
//
LOCUS       DANRDI010000333          612 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.340, whole genome shotgun sequence.
ACCESSION   DANRDI010000333 DANRDI010000000
VERSION     DANRDI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.340"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..479)
                     /locus_tag="PZ377_002919"
     CDS             complement(<1..479)
                     /locus_tag="PZ377_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDM6359752.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     "
BASE COUNT          224 a           88 c           65 g          235 t
ORIGIN      
        1 gtaaataggt aataaaaaat tgtgaaacaa atatttttaa aggagggtaa taatcttaat
       61 ttaagttcat tatttttact ttcattataa atatttaatg ttaatttctt atttagataa
      121 caataaaaaa atataatctc taaaaaccca atgaaacagc tgctccaaac aattagttct
      181 tctaattgaa aatcaaataa ataagtattt cttcttacag taccaccaag taacacaata
      241 cccgcgaata tggtccatcc tatcgattta tttttaactg gtttgacaat atttaatttt
      301 tcataatcat tcttagtgat tttgacatat gttttcggta tgagccagtt aataaatgga
      361 aagaagaaga caatccaatt acttgccaaa tcaatcatta aatattcact atcgtatttg
      421 attattcgat atttagggtt tttattaata actttagatt cgcaaagcaa tgtctccaca
      481 tccctttaat tttatgtgta atacattttt cgatacttca aaagacattc aaatactatc
      541 aagttactgt catcaaaggt tttattaact gatattttca tatttttaat ctgaatttag
      601 tactttcact ta
//
LOCUS       DANRDI010000334          605 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.341, whole genome shotgun sequence.
ACCESSION   DANRDI010000334 DANRDI010000000
VERSION     DANRDI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.341"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..159)
                     /locus_tag="PZ377_002920"
     CDS             complement(<1..159)
                     /locus_tag="PZ377_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinylbenzoate-CoA ligase"
                     /protein_id="HDM6359753.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYI"
     gene            complement(318..>605)
                     /locus_tag="PZ377_002921"
     CDS             complement(318..>605)
                     /locus_tag="PZ377_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDM6359754.1"
                     /translation="KYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYV
                     YLIENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT          183 a           96 c           86 g          240 t
ORIGIN      
        1 tatgtatagc ccaacacgag attgttgata agccttgagt cttttagcca atagactcgc
       61 ttcacagtat aaattttgat aagtataaga ttcttgaccg tctgttatcg caatatgatg
      121 tccattttgt tgtgcttgtt tatataacca aaagtccatg cgttattcct ccaaaatcat
      181 ttacattata attataacga ttttatgaca ttctagcagt ggttatgttt aaaaatataa
      241 aaaagtagac gaattgatgc attgatatga ttgttataat gctcaataca tatcgttata
      301 tcattcgtct actattatca gttattttta tttaatttta gtgtcattct gtcattttga
      361 tgtggtgatt tacccattgt tgccacatca tctgcaatgt caattggtat acggttcatg
      421 tcttgtaatg cacttaaatg gaatacttca tcatctaaat tttcaatgag atatacataa
      481 tatgttacct tgtccttttt atattttaac gttttccaaa agtccggctt gcaattcaat
      541 acattatccg gaatatattc aataaataag taacgtttgc tgcctacttt gtctatgaaa
      601 tattt
//
LOCUS       DANRDI010000335          596 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.342, whole genome shotgun sequence.
ACCESSION   DANRDI010000335 DANRDI010000000
VERSION     DANRDI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.342"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(22..>596)
                     /locus_tag="PZ377_002922"
     CDS             complement(22..>596)
                     /locus_tag="PZ377_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDM6359755.1"
                     /translation="NASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQ
                     LGTRVVIGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFL
                     INNESASGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDT
                     QKVLPNKIQALGFQFKYSNLKMALEDLIKE"
BASE COUNT          202 a           99 c           96 g          199 t
ORIGIN      
        1 aatgctcatt aatggtattg attattcttt aattaaatct tcaagtgcca tttttaaatt
       61 actatattta aattggaatc ccaatgcttg aattttatta ggtaatactt tttgagtatc
      121 caatactact gttgacattt gaccaagtat gagacgcatt gcaagacttg gtgcccaagt
      181 ttcatgaggc ttatgcatag ctcttgctaa agtgtagcca aataaatttt gacgttcagg
      241 tataggtgca gttaaattaa acggaccact agctgactcg ttatttatta aaaataaaat
      301 agcttgaatt aaatcattga tatgaatcca tgaataccat tgttgaccag aacctaattt
      361 accaccaatg taatattcgt atggtagttt cattgtttgt aacgcaccgc cttcattcga
      421 taaaattata ccgaaacgac cgatgacaac tcgtgtacct aattgttcaa attgttgtgc
      481 gaaacgttcc cattgataca caatatctga taagaaatca aaaggtaaag ttttataaac
      541 ttctgtgtaa ctcataaata aatcaggagg atagtaacca gtggcactag cattaa
//
LOCUS       DANRDI010000336          593 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.343, whole genome shotgun sequence.
ACCESSION   DANRDI010000336 DANRDI010000000
VERSION     DANRDI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.343"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..360
                     /gene="lpl5"
                     /locus_tag="PZ377_002923"
     CDS             <1..360
                     /gene="lpl5"
                     /locus_tag="PZ377_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HDM6359756.1"
                     /translation="ILLDKVEDPNLKKRIENFKFFGQYANFKDLENYNNGDVSINWNV
                     PSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHIDGDLKGSSVGYKRLEIDFSKE
                     GRDISVIDYLSYKPAKK"
     gene            392..>593
                     /gene="lpl6"
                     /locus_tag="PZ377_002924"
     CDS             392..>593
                     /gene="lpl6"
                     /locus_tag="PZ377_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HDM6359757.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGY"
BASE COUNT          242 a           60 c          115 g          176 t
ORIGIN      
        1 atcttattag acaaggtaga agatccaaat ctaaaaaaga gaatagaaaa ctttaaattt
       61 ttcggacaat atgcaaattt taaggatttg gaaaattaca acaatggcga cgtgtcaata
      121 aattggaatg ttccaagtta tgacgtggaa tataaaatga gcaataaaga tgaaaatgtt
      181 aaacaattaa gaagtcgtta taacattcct actgataaag ctccaatgtt aaaaatgcat
      241 attgacgggg acttaaaagg tagttctgtt ggatataaaa ggttagaaat agatttttca
      301 aaagaaggta gggatatttc agtcattgat tatttaagtt ataagccagc gaaaaaatag
      361 tactgataat taaatattag ggtgtgaagg aatggaatat ctaaaaaggc ttgcattgtt
      421 aataagtgtt attattttga ccatttttat aatgggttgt gatagtcaaa gcgatactgc
      481 agaaaatcca aaagaaggtt caaaagaagc acaaattaaa aagagttttt cgaaaacgtt
      541 agatatgtat ccaattaaga atctcgagga tttttatggc aaagaaggat atc
//
LOCUS       DANRDI010000337          590 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.344, whole genome shotgun sequence.
ACCESSION   DANRDI010000337 DANRDI010000000
VERSION     DANRDI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.344"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            54..>590
                     /locus_tag="PZ377_002925"
     CDS             54..>590
                     /locus_tag="PZ377_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDM6359758.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGL"
BASE COUNT          243 a           68 c          101 g          178 t
ORIGIN      
        1 tcatataaaa atatattaag acaaatttat aaatagattg ggagaatagt actgtgaaat
       61 taaaaacgtt agctaaagca acattggcat taggcttatt aactactggt gtgattacat
      121 cagaaggcca agcagttcaa gcaaaagaaa agcaagagag agtacaacat ttatatgata
      181 ttaaagactt acatcgatac tactcatcag aaagttttga attcagtaat attagtggta
      241 aggttgaaaa ttataacggt tctaacgttg tacgctttaa ccaagaaaat caaaatcacc
      301 aattattctt attaggtaaa gataaagaga aatataaaga aggcattgaa ggcaaagatg
      361 tctttgtggt aaaagaatta attgatccaa acggtagatt atctactgtt ggtggtgtga
      421 ctaagaaaaa taacaaatct tctgaaacta atacacattt atttgttaat aaagtgtatg
      481 gcggaaattt agatgcatca attgactcat tttcaattaa taaagaagaa gtttcactga
      541 aagaacttga tttcaaaatt agacaacatt tagttaaaaa ttatggttta
//
LOCUS       DANRDI010000338          584 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.345, whole genome shotgun sequence.
ACCESSION   DANRDI010000338 DANRDI010000000
VERSION     DANRDI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.345"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..136)
                     /locus_tag="PZ377_002926"
     CDS             complement(<1..136)
                     /locus_tag="PZ377_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDM6359759.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     L"
     gene            complement(252..>584)
                     /locus_tag="PZ377_002927"
     CDS             complement(252..>584)
                     /locus_tag="PZ377_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDM6359760.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNN
                     EQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLFRKR
                     KQDHKEKA"
BASE COUNT          160 a          105 c           99 g          220 t
ORIGIN      
        1 gtaacaacat taaagttttt aatggtttaa cagttgtatc gctattaaag aagctttgat
       61 cttccggtaa aatgactgtc aaattttgat gcataccaat tgttggtgaa tgatagttta
      121 atgaaatata agccatacgt catgacccct ttctaattct actttatcaa cattttacgc
      181 ttaatcaatt cactttaaaa tcattttcaa caaaaaaacc gaatacaaat gtattcggcc
      241 taaaaaagta tttacgcttt ttctttatga tcttgcttgc gttttctaaa caatagtaat
      301 gatcctaata atgccatcat tgcaccaaat aaagttgcat ttgtgttttc gctcttatct
      361 cctgtttctg gtaaagcatc agttttgtgt tgttttgata ccttattaga atggtttact
      421 tcacctttag gatttgatgg tgctttctgt tcattatttg gtggtgtaac tcttgaatcg
      481 gagtcactat ctgagtctga gtcgctatct gaatccgagt cgctatccga gtctgagtcg
      541 ctatctgagt ctgaatcgct gtctgagtct gagtcgctat ccga
//
LOCUS       DANRDI010000339          577 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.346, whole genome shotgun sequence.
ACCESSION   DANRDI010000339 DANRDI010000000
VERSION     DANRDI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.346"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            117..437
                     /locus_tag="PZ377_002928"
     CDS             117..437
                     /locus_tag="PZ377_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDM6359761.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            434..>577
                     /locus_tag="PZ377_002929"
     CDS             434..>577
                     /locus_tag="PZ377_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HDM6359762.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKE"
BASE COUNT          217 a           96 c          103 g          161 t
ORIGIN      
        1 aatctaaaga agtaacgatt tctttatcta aatattgagt gagcaatctc ttatgcatac
       61 ttatcacctc ctttcactag gagataacaa cattatacac gaaaggaaag atagaaatgc
      121 cacacatttt aaacgtaaca gttccaatac ctgaaacaca tgtacttatc acaaaagatg
      181 aatatgatga gctaattggt tattcattag accctgtatg gaacatgagt gacttaaaga
      241 agaaattaaa aattgcatct gatgagacta tcaaggacag attactattt catcctagat
      301 ttgaaaaaga actaagagcg caaggaattg tgcattaccc agatgagagt tttaatcgct
      361 ggagatttaa cgcaagaaag atgaataaat tcgtcgatga gcatttcaat gaaatatata
      421 aggagagaat aaaatgagcg acacatataa aagctaccta ttagcagtgc tatgtttcac
      481 agtcttagcg attgtgctta tgccattgct gtacttcact acagcgtggt caattgcagg
      541 attcgcaagc attggaacat tcatatacta caaagaa
//
LOCUS       DANRDI010000340          569 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.347, whole genome shotgun sequence.
ACCESSION   DANRDI010000340 DANRDI010000000
VERSION     DANRDI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.347"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>569
                     /locus_tag="PZ377_002930"
     CDS             <1..>569
                     /locus_tag="PZ377_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDM6359763.1"
                     /translation="PTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRF
                     MNVVPDYFIYKIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKK
                     VDHKAGVRITKEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSG
                     KIVIKMKNGGKYTFELHKKLQENRMADVI"
BASE COUNT          244 a           62 c          101 g          162 t
ORIGIN      
        1 ccaaccacaa aacaagtacc aacagaaata aatcctaaat ttaaagattt aagagcgtat
       61 tatacgaaac caagtttaga atttaaaaat gagattggta ttattttaaa aaaatggacg
      121 acaataagat ttatgaatgt tgtcccagat tatttcatat ataaaattgc tttagttggt
      181 aaagatgata aaaaatatgg tgaaggagta cataggaatg tcgatgtatt tgtcgtttta
      241 gaagaaaata attacaatct ggaaaaatat tctgtcggtg gtatcacaaa gagtaatagt
      301 aaaaaagttg atcacaaagc aggagtaaga attactaagg aagataataa aggtacaatc
      361 tctcatgatg tttcagaatt caagattact aaagaacaga tttccttgaa agaacttgat
      421 tttaaattga gaaaacaact tattgaaaaa aataatctgt acggtaacgt tggttcaggt
      481 aaaattgtta ttaaaatgaa aaacggtgga aagtacacgt ttgaattgca caaaaaatta
      541 caagaaaatc gcatggcaga tgtcataga
//
LOCUS       DANRDI010000341          563 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.348, whole genome shotgun sequence.
ACCESSION   DANRDI010000341 DANRDI010000000
VERSION     DANRDI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.348"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..143
                     /locus_tag="PZ377_002931"
     CDS             <1..143
                     /locus_tag="PZ377_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDM6359764.1"
                     /translation="AKSNQRFTVVEAESKEEAERKYEAQVKRDAVIKVGQLFENIREC
                     GK"
     gene            140..343
                     /locus_tag="PZ377_002932"
     CDS             140..343
                     /locus_tag="PZ377_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359765.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            336..>563
                     /locus_tag="PZ377_002933"
     CDS             336..>563
                     /locus_tag="PZ377_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359766.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDN"
BASE COUNT          230 a           60 c          118 g          155 t
ORIGIN      
        1 aagctaaaag caatcaaagg tttacagttg ttgaggcaga gagtaaagaa gaagctgagc
       61 gcaaatacga ggcacaagtt aaaagagatg cagttattaa agtgggtcag ttatttgaaa
      121 atataaggga gtgtgggaaa tgacggaggt taaaattaaa actatttcag atagagttta
      181 ttacacaaca acagatctag cttctgacga ttatattaat cttgttatga atctagtgat
      241 tgaggatttt cttccggtca aagatgtgtt caacaatgaa gtatgggtta aaagagatga
      301 gattgaatca tttacattta ttaaggaggc aaacgatgat taacatacct aaaatgaaat
      361 tcccgaaaaa gtacactgaa ataatcaaaa aatataaaaa taaagcacct gaagaaaagg
      421 ctaagattga agatgatttt attaaagaaa ttaaagataa agacagtgaa ttttacagtc
      481 ctacgatggc taatatgaat gaatatgaat taagggctat gttaagaatg atgcctagtt
      541 taattgatac tggagatgac aat
//
LOCUS       DANRDI010000342          563 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.349, whole genome shotgun sequence.
ACCESSION   DANRDI010000342 DANRDI010000000
VERSION     DANRDI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.349"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..488
                     /locus_tag="PZ377_002934"
     CDS             <1..488
                     /locus_tag="PZ377_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDM6359767.1"
                     /translation="NDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNF
                     DDKEMLLQEFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEI
                     LTSLKRLKKAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFN
                     Q"
BASE COUNT          242 a           75 c           76 g          170 t
ORIGIN      
        1 ctaatgatac tgactttaat gacattgact ttaatgatac tgagaataat gatatgaatg
       61 acttgaatga tataaagtac aaaaatgaaa ttagtaatca ttcaaatcat tcaaatcaat
      121 ttacacataa ttttgatgat aaagaaatgc tattgcaaga attcccagaa caactaacta
      181 attatttatt aaaatatgat tatagagatt tagaaatcat aaaggctgtt attcttaaag
      241 caaaaaaatc atttaattca aggcatgaag atatgcatta catgttagag gacattgagg
      301 atgaaatatt aacttcttta aaaagactta aaaaagctat acatgatagg ggagttaaag
      361 gccaaaagga aacaatcaag tcaatgcaag cttatttaat gcaaacaatt ctaactgaac
      421 ttgaagaaac acatgccctt tatatgcgcc gtaaaaacat gaaacaatat aatatcttta
      481 atcaataatt caaatgattt tataacactc gaagagacat caaaatattc cgttaaatca
      541 tctgactttt taactgatga cga
//
LOCUS       DANRDI010000343          560 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.350, whole genome shotgun sequence.
ACCESSION   DANRDI010000343 DANRDI010000000
VERSION     DANRDI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.350"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(256..>560)
                     /locus_tag="PZ377_002935"
     CDS             complement(256..>560)
                     /locus_tag="PZ377_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDM6359768.1"
                     /translation="IKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFYATEFDFP
                     KALRKEEIVGSIENDEIQLVDDYVEFIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
BASE COUNT          190 a           90 c           65 g          215 t
ORIGIN      
        1 atgtaaatta tagttagtta ttaccaaaat gtaaatatac actatttttc aagaattgaa
       61 ccgctttttc atttaaattt ttcaatattg ctaagcataa ttgatggata ctttaacaac
      121 ccattactgc tcggcaaaat taataatggc aagaaattga accttataaa cacatacgat
      181 ttagagcata aaaaataacc atgaagctct acctattgat taaatagatt cttcatggct
      241 attttagttt tagttttata atgcttcaaa gtctaatttt gatttaactt cacttatgaa
      301 atacagacta ccggtaatta ctaatgtatc accttgataa ttttttataa attcaacgta
      361 gtcatctact aattgtattt catcattttc aatactacct acaatttctt ctttgcgtaa
      421 cgctttcgga aaatcaaatt cagttgcata aaacgtatgc gcaattaaac ttaaatgttt
      481 gaccatctcg ttaatcggtt ttccgtttat tgctgagaac aaaatatcta ctttttcttt
      541 atcatggtac tgtttaattg
//
LOCUS       DANRDI010000344          557 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.351, whole genome shotgun sequence.
ACCESSION   DANRDI010000344 DANRDI010000000
VERSION     DANRDI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.351"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>557
                     /locus_tag="PZ377_002936"
     CDS             <1..>557
                     /locus_tag="PZ377_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDM6359769.1"
                     /translation="PTIKQAQTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRF
                     MNVIPNRFIYKIALVGKDEKKYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKK
                     VNHKVELSITKKDNQGMISRDVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSG
                     TIVIKMKNGGKYTFELHKKLQEHRMA"
BASE COUNT          230 a           72 c           99 g          156 t
ORIGIN      
        1 ccaaccataa aacaagcaca aacagatatg actcctaaat atgaagattt aagagcgtat
       61 tatacaaaac cgagttttga atttgaaaag cagtttggat ttatgctcaa accatggacg
      121 acggttaggt ttatgaatgt tattccaaat aggttcatct ataaaatagc tttagttgga
      181 aaagatgaga aaaaatataa agatggacct tacgataata tcgatgtatt tatcgtttta
      241 gaagacaata aatatcaatt gaaaaaatat tctgtcggtg gcatcacgaa gactaatagt
      301 aaaaaagtta atcacaaagt agaattaagc attactaaaa aagataatca aggtatgatt
      361 tcacgcgatg tttcagaata catgattact aaggaagaga tttccttgaa agagcttgat
      421 tttaaattga gaaaacaact tattgaaaaa cataatcttt acggtaacat gggttcagga
      481 acaatcgtta ttaaaatgaa aaacggtggg aaatatacgt ttgaattaca caaaaaactg
      541 caagagcatc gtatggc
//
LOCUS       DANRDI010000345          545 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.353, whole genome shotgun sequence.
ACCESSION   DANRDI010000345 DANRDI010000000
VERSION     DANRDI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.353"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..241)
                     /locus_tag="PZ377_002937"
     CDS             complement(<1..241)
                     /locus_tag="PZ377_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDM6359770.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPP"
     gene            complement(350..487)
                     /locus_tag="PZ377_002938"
     CDS             complement(350..487)
                     /locus_tag="PZ377_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359771.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
BASE COUNT          138 a           82 c          113 g          212 t
ORIGIN      
        1 aaggcggtgt tgtcgcattt gctgtttgtt gcggtgcttc tactttagtt gagggcggtg
       61 ttgtcgcgtt tggttttgat tgcggtgctt ctattttagt tgagggcggt gttgattgtg
      121 gtgcttccac tttagtggaa gatggtgttg tcgcgtttgc tgcttgcgtt gtcgttgtga
      181 ttacacctgt tgttaaaagg cctagtgcta aacttgtttt agcaatcgtt gttattttca
      241 tagttgtatg ctccattcgt aattattaga tttgttcgat tacattcatt gaatcataca
      301 gctttattat agatggcgta ttgctccatt cacattaaac cttgtttaac tatatttgaa
      361 tcatcgttaa gtaaattaag aaatccataa tgttcgttaa ataaaaatga ttttgatgtg
      421 attcaacact tggcacattt gaagtttcgt cacttttaag acatagaaat gccactttta
      481 caaacaaatg aatattcgtc tttttacatc attacgcata ataaaagaag ctaagcaaca
      541 tgtaa
//
LOCUS       DANRDI010000346          535 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.354, whole genome shotgun sequence.
ACCESSION   DANRDI010000346 DANRDI010000000
VERSION     DANRDI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.354"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..446
                     /locus_tag="PZ377_002939"
     CDS             <1..446
                     /locus_tag="PZ377_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDM6359772.1"
                     /translation="YVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSA
                     AKLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLII
                     GLIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
BASE COUNT          166 a           84 c          101 g          184 t
ORIGIN      
        1 catatgtcgc aatcatattc ctagtaggag tgtcgttaat tgcaccttgg ctaggaagaa
       61 ctgcattgac ttggattgta gatatgtcat ctactggtgt atccattgcc tactttatta
      121 catgtttgtc tgcagcgaaa ttattcagtt ataacaaaca aagtaatacg tatgcaccgg
      181 tttacaaaac gtttgctatt atcggctcat ttgtatcatt cattttctta gcgttgttat
      241 tagtgccagg ttctcctgca gcactgactg caccgtctta tattgcatta cttggatggt
      301 taatcatcgg tttaatattc tttgtgattc gatatcctaa attgaaaaat atggataatg
      361 atgaattaag tcgcttgatt ttaaatagaa gtgaaaatga agttgatgat atgattgaag
      421 aacctgaaaa agaaaaaact aaataataaa agaatcgcac aataaacctt cttcattcgg
      481 aggcgtatcg tgcgattttt tgtattataa attgacattt aagacgaggc agctg
//
LOCUS       DANRDI010000347          520 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.355, whole genome shotgun sequence.
ACCESSION   DANRDI010000347 DANRDI010000000
VERSION     DANRDI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.355"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(61..144)
                     /locus_tag="PZ377_002940"
     tRNA            complement(61..144)
                     /locus_tag="PZ377_002940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Leu,seq:caa)
     gene            complement(183..257)
                     /locus_tag="PZ377_002941"
     tRNA            complement(183..257)
                     /locus_tag="PZ377_002941"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Gly,seq:tcc)
     gene            complement(361..435)
                     /locus_tag="PZ377_002942"
     tRNA            complement(361..435)
                     /locus_tag="PZ377_002942"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(400..402),aa:Gly,seq:tcc)
     gene            complement(443..516)
                     /locus_tag="PZ377_002943"
     tRNA            complement(443..516)
                     /locus_tag="PZ377_002943"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Cys,seq:gca)
BASE COUNT          157 a          111 c          115 g          137 t
ORIGIN      
        1 aaagaaacct tgttttacaa ggtttctaat acgttatgtt atgtaaataa cagttaatta
       61 taccggtggt cggggtcgaa ccgacactcc acaagtggaa cgggattttg agtcccgcgc
      121 gtctgccaat tccgccacac cggcttaatg gtaaacaaaa aacttccctt tggaagcaat
      181 tatggagcgg aagataggat ttacacctat acctcgttcc gggaaggaac gtgttctaaa
      241 agttgaacta ctcccgcaaa tattaaatta tggagcggaa gataggattt acacctatac
      301 ctcattccag gaaggaatgt attctaagag ttgaaatact cccgcattat tattaaatta
      361 tggagcggaa gataggattt gcacctatac ctcgttccgg gaaggaacgt gttctaaaag
      421 ttgaactact cccgcataaa cctggaggcg gcaaccggat ttgaaccggt gataaaggtt
      481 ttgcagacct ctgccttacc acttggctat gccgccaata
//
LOCUS       DANRDI010000348          502 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.356, whole genome shotgun sequence.
ACCESSION   DANRDI010000348 DANRDI010000000
VERSION     DANRDI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.356"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            320..409
                     /locus_tag="PZ377_002944"
                     /pseudo
     CDS             320..409
                     /locus_tag="PZ377_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            420..>502
                     /locus_tag="PZ377_002945"
     CDS             420..>502
                     /locus_tag="PZ377_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDM6359773.1"
                     /translation="MIFEEKLNEMYNEIANKISSMIPVEWE"
BASE COUNT          213 a           48 c           98 g          143 t
ORIGIN      
        1 aattgcatat gtggacgatg gaggtgggca agttatattt tattatacca agcccagaaa
       61 tgatgagctg tattattatt cgagtatcgt tgaagattat aatgttttag aagaaatctt
      121 tgatgattta tggatggaac tttatagatc atttaaaaaa ttaagaaata tatttaaaga
      181 agaaagtctt gaaccatgga catcatgtga atttgacttt acaaaagaag ataaattaaa
      241 agtttcattt gattatattg attggaagaa tacagaattt gatcaattag gccaagaaaa
      301 ctactataac tatagaaaat tcggaattat accagaaatg gaatatgaaa tggaagaggt
      361 taaacaaatc gagcaatata ttaaagagca agaagaagct gaacaataga ggcgataaca
      421 tgattttcga agaaaaacta aatgaaatgt acaacgagat tgcgaataaa attagtagca
      481 tgataccagt agaatgggaa aa
//
LOCUS       DANRDI010000349          482 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.357, whole genome shotgun sequence.
ACCESSION   DANRDI010000349 DANRDI010000000
VERSION     DANRDI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.357"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(84..218)
                     /locus_tag="PZ377_002946"
     CDS             complement(84..218)
                     /locus_tag="PZ377_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359774.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            complement(283..>482)
                     /gene="splF"
                     /locus_tag="PZ377_002947"
     CDS             complement(283..>482)
                     /gene="splF"
                     /locus_tag="PZ377_002947"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDM6359775.1"
                     /translation="SVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
BASE COUNT          160 a           63 c           81 g          178 t
ORIGIN      
        1 gataatttgg atgttggtgg tggaaatttt gagttttagt ggcgcaattg ttattgaaaa
       61 attttataga aatgttgtag ctttcaaatg ctttcaaaat catttatatt cttaatgatg
      121 tcaaaaagtt gttcattcat acataaataa aaccaatcaa caattgagtt ggtgaaaatc
      181 aatcgttgat tggctttgat gcatattaaa taatgcaata tatattaata aatgttagtt
      241 atagtatatt ttgtcaggat tgggtgaatg tctaagtttt aattatttat ctaaattatc
      301 tgcaatgaat ttcttaattt caggagagaa ataaacagca aatcctcttg tgctttcacc
      361 tgatggctta ttaccggcat agattacacc aatagcttcg tgtttactat ttaatatagg
      421 tgaaccagag ctaccaggct gaataattgc atccgatgac actatattcc cattcactga
      481 ta
//
LOCUS       DANRDI010000350          479 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.358, whole genome shotgun sequence.
ACCESSION   DANRDI010000350 DANRDI010000000
VERSION     DANRDI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.358"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>479)
                     /locus_tag="PZ377_002948"
     CDS             complement(<1..>479)
                     /locus_tag="PZ377_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDM6359776.1"
                     /translation="FPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQ
                     NIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLN
                     QHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTI"
BASE COUNT          140 a           91 c           55 g          193 t
ORIGIN      
        1 aatatagttg gggtgaagat ttttaaatta gcaatttctt tgaagtttag accttctcga
       61 ctacctccac tttgtgcaag gaaaattttc cttttacctt ttcttgataa tagatactgt
      121 ccaaaaaaat tataataata ctcttttttc aatctaataa tacatacatg ttgatttaaa
      181 ttagcatgcg tttcaactat cgaattaatg gctgttctac ctattgatgc tcctgtaata
      241 tttaaaagaa catcaccata gtacgttcta ctatttttca tctcatcatc tatatcttta
      301 ctaatataaa ctaagtcatt aagatttaat ttaccatttc taatattttg actccttaaa
      361 aatggtatgc ctttgtttgt atagttttca cttccacctt tgggagtctt tccactacct
      421 attttggtag taagattccc taacttcttc tcttcccatt cgccttcaaa tcctgggaa
//
LOCUS       DANRDI010000351          478 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.359, whole genome shotgun sequence.
ACCESSION   DANRDI010000351 DANRDI010000000
VERSION     DANRDI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.359"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(331..>478)
                     /locus_tag="PZ377_002949"
     CDS             complement(331..>478)
                     /locus_tag="PZ377_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunoglobulin-binding protein sbi"
                     /protein_id="HDM6359777.1"
                     /translation="NYVTESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLL
                     SFWK"
BASE COUNT          165 a           42 c           96 g          175 t
ORIGIN      
        1 gctttgcaag tcttatttta ttgaattgtt gtttaattat ggtgcttgta gtggaaaagg
       61 aatggattgc gcaatagttt gtgaaaatat gaagttaata aagttgagct gattcaacta
      121 aatgatgaac tattcgttaa cgatgtaagg attatttaac aagttgcagg taaaaaagtg
      181 ggctgacaaa tagcgaagaa gcatttaatt gatggaaaat gaaaaaagaa ccgtagattc
      241 gattgagaat caacggttct ttttaatcac ggtttgttac gaaaattatt taaaaaatga
      301 tgtaaaacta gagaagatat ttttgattga ttatttccag aatgataata aattacttaa
      361 tgtatttttg attgatgatg cagtttcttg agctttaata gctgttttta ctaatgttgg
      421 gttttggtag aaagtatata atactttacc agtattgatt gactcagtta catagttt
//
LOCUS       DANRDI010000352          474 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.360, whole genome shotgun sequence.
ACCESSION   DANRDI010000352 DANRDI010000000
VERSION     DANRDI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.360"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(41..277)
                     /locus_tag="PZ377_002950"
     CDS             complement(41..277)
                     /locus_tag="PZ377_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359778.1"
                     /translation="MFNTPKMKLPEKHTEVFKTYKNGTPEEKAEIEGCFIKTVKDEDS
                     EFYSPMLASLNEQQLKSMLRQVLFLIDTGDDNDD"
     gene            complement(302..>474)
                     /locus_tag="PZ377_002951"
     CDS             complement(302..>474)
                     /locus_tag="PZ377_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDM6359779.1"
                     /translation="AKSNQRFTVVEAESKEEAKEKYESQNTPIVYYTNNSKVTLFERP
                     SEEVLGSLFEKK"
BASE COUNT          127 a           95 c           57 g          195 t
ORIGIN      
        1 ctcatacatc gctagtaaga atattagtct taatatttgt ttaatcatca ttgtcatctc
       61 ctgtatcaat caaaaaaagt acctgtctca acatactctt taactgttgt tcatttagac
      121 tggctaacat agggctgtaa aattcactat cttcatcttt aacagtttta ataaaacagc
      181 cttcaatctc agctttttct tctggcgttc catttttata cgtcttaaat acctcggtgt
      241 gcttttctgg taatttcatt ttaggtgtat taaacattat tatctcccct ctttaatgat
      301 tttatttctt ttcgaacaaa gaacctaata cttcttcact aggtctttcg aataaggtca
      361 ctttagaatt attagtgtag taaacaatag gtgtattttg tgactcatat ttctctttcg
      421 cttcttcttt actctctgcc tcaacaactg taaacctttg attgctttta gctc
//
LOCUS       DANRDI010000353          467 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.361, whole genome shotgun sequence.
ACCESSION   DANRDI010000353 DANRDI010000000
VERSION     DANRDI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.361"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>467)
                     /locus_tag="PZ377_002952"
     CDS             complement(<1..>467)
                     /locus_tag="PZ377_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDM6359780.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPS"
BASE COUNT          152 a           92 c           54 g          169 t
ORIGIN      
        1 aaacttggat atttaattag aatagtaaaa aaatcattca cagaaacatt taataatccg
       61 tgatttcttg caccctcaac tgcaattcca gaaacttctc tataccagtg tgtcgaatca
      121 aaatatgctt ccatgaagtc tttagacatt tcacttttaa tagaaaaaca aatatacaaa
      181 gaggacaata caccactatc atatctagtt aatcttttaa tagcccctaa tgggtatcca
      241 ttagaataac ttttgttata cgcgaattct ccattcttta ttagtgtata attttctaga
      301 tttttcgacg aaactgattt actaaaatat tctgtttgat caattaaacc taactgtccg
      361 gatattgtta aaggcttttt cgattctaag tttttatttt tcctaattac tctatctgta
      421 agatccccta actgcttctc ttcccattcg ccttcaaacc ctgggaa
//
LOCUS       DANRDI010000354          446 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.362, whole genome shotgun sequence.
ACCESSION   DANRDI010000354 DANRDI010000000
VERSION     DANRDI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.362"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>446
                     /locus_tag="PZ377_002953"
     CDS             <1..>446
                     /locus_tag="PZ377_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDM6359781.1"
                     /translation="LLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYT
                     LPLLFVSIYVFGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAI
                     AIIIKLFAFGGIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRS"
BASE COUNT          132 a           46 c           78 g          190 t
ORIGIN      
        1 tttactggtg tttatggcct ttacagctgt aattttgatt atgatgtaca tacaagagaa
       61 aaatgaaaat tacaaagttg aaaaaagata tgtggttaga tatctcacac ttaacgttat
      121 tgtgggatat actttgccat tgctttttgt atctatttac gtttttggtg tagtcggttt
      181 tggatttgat gttttcaatt attgtctagg tattatcttg atgttattta tttcttggtt
      241 aggtttattt ttattttata aaaacgaatt tgatagtgaa aatcctaatc ctgcagttaa
      301 tgccatagca attattataa aattatttgc ttttggagga atattttata taagtctaat
      361 agttcctgta acacagcaag aagagatttt tatagggtta agtatattca tcaatataat
      421 tgttgatgct cttcttgtta ggtcat
//
LOCUS       DANRDI010000355          444 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.363, whole genome shotgun sequence.
ACCESSION   DANRDI010000355 DANRDI010000000
VERSION     DANRDI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.363"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>444
                     /locus_tag="PZ377_002954"
     CDS             <1..>444
                     /locus_tag="PZ377_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HDM6359782.1"
                     /translation="PISWTPFGELFSGVFMGMFIIVIAFFIQTGNIQSYVIWLSVPIV
                     ITIGLINMANNIRDRVKDKASGRKTLPILLGKNASLTFMAIMYFIAYAFIVLTIIIKP
                     GGSLFYLLALLSFPMPVKVIRRFKKNDTPPTMMPAMAAAGKTNTF"
BASE COUNT          127 a           81 c           73 g          163 t
ORIGIN      
        1 ccctatttca tggacgcctt tcggtgaatt attctcaggc gtatttatgg gtatgtttat
       61 tatcgttatt gcattcttta ttcaaactgg caatattcaa agttatgtaa tttggttaag
      121 tgtacctata gtaatcacta tcggtttaat taatatggct aacaatattc gcgaccgtgt
      181 caaagataaa gcaagtggtc gcaaaacttt acccattcta ttaggtaaaa atgcttcttt
      241 aacatttatg gcaatcatgt actttatcgc ttatgccttt attgtactta cgatcattat
      301 taaacctggt ggctcattat tttacttact tgcgttgtta tcattcccaa tgcctgttaa
      361 agttatcaga cgtttcaaga agaatgatac accgcctaca atgatgccag caatggctgc
      421 tgctggtaaa acaaatacat tttt
//
LOCUS       DANRDI010000356          444 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.364, whole genome shotgun sequence.
ACCESSION   DANRDI010000356 DANRDI010000000
VERSION     DANRDI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.364"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            231..368
                     /locus_tag="PZ377_002955"
     CDS             231..368
                     /locus_tag="PZ377_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359783.1"
                     /translation="MILIMLDSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKD
                     K"
BASE COUNT          189 a           40 c           65 g          150 t
ORIGIN      
        1 tcagaatatg atttatttac attttttaag catccatcaa taaaatcaat gacaatcgtt
       61 actaataagg gacaagtaaa atttattacc aagtcaaata ggtttcatgg taaaatagtg
      121 agtaaatttt gtgctaaata ttttacgcat attaatatca ttaatgatag ccatattgaa
      181 aaattattga aaaaacttta tagtataaat atgattaaat ataaagtgag atgattttaa
      241 ttatgttaga ttcacaatta gatggaaatg taactgcaaa agaattgagt gaatctttta
      301 aagaattagt tgaagaattt gaaagtatag aaaaagcgaa aaagaattca aattcaaaag
      361 ataaatagca tcctttttag acagataaag agaaagtggt gctattttta tacgcttttt
      421 taactattta ctgaaaatgc agaa
//
LOCUS       DANRDI010000357          433 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.365, whole genome shotgun sequence.
ACCESSION   DANRDI010000357 DANRDI010000000
VERSION     DANRDI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.365"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(5..166)
                     /locus_tag="PZ377_002956"
     CDS             complement(5..166)
                     /locus_tag="PZ377_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359784.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
BASE COUNT          147 a           72 c           53 g          161 t
ORIGIN      
        1 gcatttactt agatactaat ttaacctttt cttcaagctg atctaacaat ccaatccatt
       61 catctatatc ttcaacacgt acttcatcag gatttacatg atcgatatcc tcaataaact
      121 tatttaaacg cgcttttatc tgttcgattg tttgttgttc attcataaaa agttaactcc
      181 ttttattttg ttttcttttt cattattatc ctaacagaaa ttgcgttaaa gcgatataat
      241 cttagctata tttatgacat tcaaattatt ttgactttta aaaatcccct tttcaattaa
      301 ctaaaattaa gagataattt gttacgagtg ataatacgaa gtggtatcat accgatatga
      361 accaaataga aagaaggaag tttaagacga tgaatagcgt caaattgaag caacctgtta
      421 gcatttacaa tga
//
LOCUS       DANRDI010000358          431 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.366, whole genome shotgun sequence.
ACCESSION   DANRDI010000358 DANRDI010000000
VERSION     DANRDI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.366"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..288
                     /locus_tag="PZ377_002957"
     CDS             <1..288
                     /locus_tag="PZ377_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDM6359785.1"
                     /translation="ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKK
                     DNFTAYATPEHNYQFGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
BASE COUNT          183 a           62 c           69 g          117 t
ORIGIN      
        1 gcgcgcatta cactttatct caagcataac actaaagaac cgaatacaat caaatctgta
       61 catttcacaa actcggaaac aagtcctatg ggaagtgctg tgattgaagg ttacataaat
      121 gaaaacaaaa aagataattt tactgcttat gctacaccag aacataatta tcaatttggt
      181 ggtgctatga tagaaagtga aaaattaagc gagttactaa agccagccaa tcagttaaaa
      241 tcaccagatg atataaaaaa agaactaaat aaaaagaaga gtcactaaag ttaggagtta
      301 ctttaatgtc caaaaaacat gtttttataa ttattggtgt catattgtgt atatgtacag
      361 tttctacggt catgcatttt aaaatgaaat atgatgaaaa agaaaaacaa aaagcgattt
      421 actacaaaga a
//
LOCUS       DANRDI010000359          419 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.367, whole genome shotgun sequence.
ACCESSION   DANRDI010000359 DANRDI010000000
VERSION     DANRDI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.367"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(99..>419)
                     /locus_tag="PZ377_002958"
     CDS             complement(99..>419)
                     /locus_tag="PZ377_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDM6359786.1"
                     /translation="LGIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIF
                     LASNGYYQHLAMNDWVSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHI
                     EVED"
BASE COUNT          145 a           81 c           56 g          137 t
ORIGIN      
        1 cattgcctaa gaacttaatg tcccagcctc cttcataact tgtataaaaa gatcattaaa
       61 aacgattcat tatatggttc gtccattcaa agtatatact aatcttcaac ttcgatatga
      121 ttaaagcgat aatagatacc atccggacct tgtaaattta aatgtgttgt ttcaggataa
      181 tgaaagtcaa caattgctaa tccatacgta tcaaaatttt ctacacgttt cgttgctgat
      241 acccaatcat tcatggccaa atgttgataa tagccattcg atgctaaaaa tattgaataa
      301 tcatccatat atgccgattg ctctaatcca aaataatcga ggtagtactc ttttacctct
      361 gataaatgaa ttgtcttaat atgcaatgca cctataatag cgtcatctgg aatacctaa
//
LOCUS       DANRDI010000360          418 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.368, whole genome shotgun sequence.
ACCESSION   DANRDI010000360 DANRDI010000000
VERSION     DANRDI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.368"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..391
                     /locus_tag="PZ377_002959"
     CDS             <1..391
                     /locus_tag="PZ377_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HDM6359787.1"
                     /translation="KVYKLFFIEAYKTVFQMTSMVSGTLKNNTDLTQILTSLFPCGSI
                     TGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDDKMIFNIPIRTIEYKYGQAIYGV
                     GAGITIDSKPKDEVNEFYAKTKILEML"
BASE COUNT          156 a           54 c           74 g          134 t
ORIGIN      
        1 taaggtgtat aaactatttt ttattgaggc atataaaact gtatttcaaa tgacttcgat
       61 ggtaagtgga actttaaaaa ataatacaga cttaactcaa attttaacat cgttatttcc
      121 ttgtggttcg attacaggtg caccgaaact gaatacaatg aaatatatta aacaattaga
      181 aagttcacct cgtggtatat actgcggagc aattggacta ttacttccaa ctgaagatga
      241 taaaatgatt tttaatattc cgattcgcac tattgagtat aaatatggac aagcgattta
      301 tggagtcgga gcaggtatta caattgattc taagccaaaa gatgaagtga atgaatttta
      361 cgcaaaaacc aagattttgg agatgttata atgcaattat ttgaaacaat gaaaattg
//
LOCUS       DANRDI010000361          386 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.369, whole genome shotgun sequence.
ACCESSION   DANRDI010000361 DANRDI010000000
VERSION     DANRDI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.369"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>386)
                     /locus_tag="PZ377_002960"
     CDS             complement(<1..>386)
                     /locus_tag="PZ377_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDM6359788.1"
                     /translation="RIPTRNLNDTVASMIIEFVEWINTLEFFKNWKKQPGKPLDTQLD
                     EIADYLAFSLQLTLTIVDEEDLEETTEVMVDLIENEVTLPKLHSVYFVHVMHTLTEQF
                     VKGIDNSIVQVLIMPFLYANTYYSID"
BASE COUNT          132 a           66 c           51 g          137 t
ORIGIN      
        1 tggtcgatag aatagtaagt attggcgtac aaaaacggca ttattaaaac ttgtacaatg
       61 ctattatcaa taccttttac aaattgttct gttagtgtat gcattacatg aacaaaataa
      121 actgaatgta gtttaggtaa agtaacttca ttttcaatca aatcaaccat aacctcagta
      181 gtttcttcca aatcttcttc atcaacaata gtcaaagtta attgcaaact gaaagctaag
      241 taatcagcaa tctcatctaa ttgtgtatct agtggcttac ctggttgttt cttccaattt
      301 ttaaaaaact caagtgtgtt aatccactct acaaattcaa taatcatact agctactgtg
      361 tcatttaaat ttctagttgg tattct
//
LOCUS       DANRDI010000362          385 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.370, whole genome shotgun sequence.
ACCESSION   DANRDI010000362 DANRDI010000000
VERSION     DANRDI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.370"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>385)
                     /locus_tag="PZ377_002961"
     CDS             complement(<1..>385)
                     /locus_tag="PZ377_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDM6359789.1"
                     /translation="KEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTH
                     LLDIQLDKKLVIAICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIILIPTLKNFCL
                     TLFRYAFFILWTVIFSYALLSMSYQN"
BASE COUNT          143 a           51 c           47 g          144 t
ORIGIN      
        1 tgttttgata actcattgat aatagagcat atgagaaaat cactgtccat aaaataaaaa
       61 aagcatatct aaacaatgtt aaacaaaaat tttttagtgt tggtattaag ataatttttg
      121 accttttatt tttagtatta taaatattta aagatgattt tttaattagg cgtacataaa
      181 atattaaaat ccctataaat gtgatgcaac atatggcaat aactaatttt ttatcaagtt
      241 gaatgtcaag taaatgtgta tattttctta atgcaactcc cagcaaagca gagctacctg
      301 caactggcca aaaaacattc tttttagcag gtttaacaat atttaagttt tcaaactctt
      361 ctctactaat tttgacgtat tcttt
//
LOCUS       DANRDI010000363          381 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.371, whole genome shotgun sequence.
ACCESSION   DANRDI010000363 DANRDI010000000
VERSION     DANRDI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.371"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>381
                     /locus_tag="PZ377_002962"
     CDS             <1..>381
                     /locus_tag="PZ377_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDM6359790.1"
                     /translation="YRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRS
                     AKGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQY
                     SNFKDLMNYKDGDISYNPEVPSYSA"
BASE COUNT          174 a           38 c           66 g          103 t
ORIGIN      
        1 tatcgtgatg atcaatttga taaaaatgat aagggaacat ggattgtaaa ttctcaaatg
       61 gcaattcaaa ataaaggaga agctctaaaa ataaaaggca tgcttttgaa gatagataga
      121 aatacaagaa gtgcaaaagg attttactat actaatgaaa taaagacgga gaaatacgaa
      181 gtagctcagg ataatcaaaa aaaatatcca gttaaaatga ttaataataa attcatttct
      241 actgaggaag ttaaagaaga aaacataaaa aaagaaatcg aaaactttaa gttttttgcg
      301 caatatagca attttaaaga tttaatgaat tataaagatg gagatatatc atataatcca
      361 gaggtgccga gttattcagc t
//
LOCUS       DANRDI010000364          374 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.372, whole genome shotgun sequence.
ACCESSION   DANRDI010000364 DANRDI010000000
VERSION     DANRDI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.372"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>374
                     /locus_tag="PZ377_002963"
     CDS             <1..>374
                     /locus_tag="PZ377_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDM6359791.1"
                     /translation="LNLRDSKIAYVVEFFEWFNTLETFKNWKKKPGKPLDVQLDELAD
                     MLAFGLSIANQVGVSSEEIKEAIESSFKNSEFHNMFNFKDKEFAQDAVVSTPQIIFKE
                     FYPDQLAIVIAIDIAFNLYSID"
BASE COUNT          137 a           50 c           76 g          111 t
ORIGIN      
        1 ctgaacttac gagatagcaa aatagcatat gtagttgaat tctttgaatg gtttaataca
       61 ttggaaacgt ttaagaactg gaagaagaaa ccaggtaagc cgttagatgt tcagttagat
      121 gaattagcgg acatgttggc gtttggattg agtattgcga atcaagtagg agtgtcatca
      181 gaagagataa aagaagcgat tgaatcaagt tttaaaaata gtgaatttca caacatgttt
      241 aattttaaag ataaagaatt tgctcaagac gcagttgtta gcacaccaca gataatattc
      301 aaagaatttt atcctgacca attggcaatt gtaatagcga tagacatagc tttcaactta
      361 tattctatcg acca
//
LOCUS       DANRDI010000365          373 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.373, whole genome shotgun sequence.
ACCESSION   DANRDI010000365 DANRDI010000000
VERSION     DANRDI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.373"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
BASE COUNT          164 a           45 c           46 g          118 t
ORIGIN      
        1 taagaatgaa attatacgaa agtatgattt aaaaccctca attatctcaa attcgataaa
       61 acaacaccaa aatactggac ccttcaatca tcaagataat ttaaaaagtg atgaaaaaga
      121 gttaataaaa ttacgcaaag aagttcaaca tttaaaaatg gaacatgatg ttttaaagca
      181 aattttaaag ttgatttaga aactgaactt tttaaagata cattacttga agattctaat
      241 ttatctgatt atataaagaa ccaacttcca acgatatgga gagactaaga aatagtttaa
      301 atttatttaa tttcaataaa aactcataat aatatttaag taagttagat tgcttttagc
      361 attaggaatt cgc
//
LOCUS       DANRDI010000366          363 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.374, whole genome shotgun sequence.
ACCESSION   DANRDI010000366 DANRDI010000000
VERSION     DANRDI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.374"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>363
                     /locus_tag="PZ377_002964"
     CDS             <1..>363
                     /locus_tag="PZ377_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDM6359792.1"
                     /translation="PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVA
                     KGMVLYMNRNTKTTNGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKI
                     KKEIENFKFFVQYGDFKNL"
BASE COUNT          159 a           42 c           66 g           96 t
ORIGIN      
        1 cctattaaaa atctagagga tttatacgat aaggaaggct atcgtgatga tcagtttgat
       61 aaaaatgata aaggtacatg gattataaat tctgaaatgg ttattcaacc taataatgaa
      121 gatatggtag ctaaaggcat ggttctatat atgaatagaa ataccaaaac aacaaatggt
      181 tactactatg tcgatgtgac taaggacgag gatgaaggaa aaccgcacga caatgaaaaa
      241 agatatccgg ttaaaatggt cgataataaa atcattccaa caaaagaaat taaagatgaa
      301 aaaataaaaa aagaaatcga aaactttaag ttctttgttc aatatggcga ctttaaaaat
      361 ttg
//
LOCUS       DANRDI010000367          359 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.375, whole genome shotgun sequence.
ACCESSION   DANRDI010000367 DANRDI010000000
VERSION     DANRDI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.375"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..170
                     /locus_tag="PZ377_002965"
     CDS             <1..170
                     /locus_tag="PZ377_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDM6359793.1"
                     /translation="VSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEID
                     QYIKEQDEAEI"
     gene            181..>359
                     /locus_tag="PZ377_002966"
     CDS             181..>359
                     /locus_tag="PZ377_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDM6359794.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSD"
BASE COUNT          150 a           29 c           76 g          104 t
ORIGIN      
        1 aagtttcatt tgattatata gattggataa atacagagtt tgatcaatta ggccgtgaaa
       61 attattatat gtataaaaaa tttggggttt taccagaaat ggaatatgaa atggaagaaa
      121 ttaaagaaat cgatcaatat attaaagagc aagatgaagc tgaaatatag gggagataac
      181 atgactttcg aagaaaagct aagtcaaatg tacaatgaaa ttgcaaatga aatcagtgga
      241 atgataccag tagaatggga aaaagtatat acaattgcct acgtagatga tgaaggtgga
      301 gaggttgttt ttaattatac taaaccagga agtgaagatt tgaattatta ttcagatat
//
LOCUS       DANRDI010000368          356 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.376, whole genome shotgun sequence.
ACCESSION   DANRDI010000368 DANRDI010000000
VERSION     DANRDI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.376"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..335
                     /locus_tag="PZ377_002967"
     CDS             <1..335
                     /locus_tag="PZ377_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDM6359795.1"
                     /translation="DVLDFTSTEKKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIE
                     QLRRDSERKEFEECIQIIRKSDSIDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLT
                     KKMGSRNT"
BASE COUNT          134 a           48 c           72 g          102 t
ORIGIN      
        1 atgatgtatt agacttcaca agtaccgaaa agaaattagg taagccggtc ggaagtgatt
       61 tgcttaatgg tcatattacg ttaccgattt tattagaaat gcgtaaaaat ccagacttca
      121 aattgaaaat cgaacagtta cgtcgtgata gtgaacgcaa agaatttgaa gaatgtatcc
      181 aaatcattag aaaatctgac agcatcgatg aggctaaggc agtaagttcg aagtatttaa
      241 gtaaagcttt gaatttgatt tctgagttac cagatggaca tccgaaatca ctacttttaa
      301 gtttgacgaa aaaaatgggt tcaagaaaca cgtagtattt atgaaaagta ttgaaa
//
LOCUS       DANRDI010000369          352 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.377, whole genome shotgun sequence.
ACCESSION   DANRDI010000369 DANRDI010000000
VERSION     DANRDI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.377"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..200
                     /gene="splD"
                     /locus_tag="PZ377_002968"
     CDS             <1..200
                     /gene="splD"
                     /locus_tag="PZ377_002968"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HDM6359796.1"
                     /translation="SVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(149..256)
                     /locus_tag="PZ377_002969"
     CDS             complement(149..256)
                     /locus_tag="PZ377_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359797.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
BASE COUNT          133 a           52 c           53 g          114 t
ORIGIN      
        1 tatcagtgaa tggaaatata gtgacatctg atgcggttgt ccaacctggc agctctggtt
       61 cacctatatt aaatagtaag cgagaagcaa ttggtgttat gtatgctagt gataaaccaa
      121 caggtgaaag tacaaggtca tttgctgttt atttctctcc tgaaattaag aaatttattg
      181 cagataattt agataaataa atcatccatc catacattga taaatgattt ttagaaatta
      241 acaacaaaat caacaatttt aaacatctct gtgattctat ttattcgaaa tgatttaaaa
      301 aataaaactt caaaaaccta accttatatt tatacgaata cttagaggag ca
//
LOCUS       DANRDI010000370          350 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.378, whole genome shotgun sequence.
ACCESSION   DANRDI010000370 DANRDI010000000
VERSION     DANRDI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.378"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..192
                     /locus_tag="PZ377_002970"
     CDS             <1..192
                     /locus_tag="PZ377_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDM6359798.1"
                     /translation="NVKQLRKRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEK
                     KEENIYFSDSLDYKKSGDV"
     gene            244..>350
                     /locus_tag="PZ377_002971"
     CDS             244..>350
                     /locus_tag="PZ377_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDM6359799.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKK"
BASE COUNT          143 a           35 c           74 g           98 t
ORIGIN      
        1 aatgtaaaac aattacgcaa aagatatgat ataccgacga gtaaagctcc aaagttattg
       61 ttaaaaggtt cagggaattt aaaaggctca tcagttggat ataaagatat tgaatttacg
      121 tttgtagaga aaaaagaaga aaatatatac tttagtgata gcttagatta taaaaaaagc
      181 ggagatgtat aatcatggct caatcagaat atgaaatcaa tcccggaaaa agagagtgat
      241 gaaatgataa aacgtgtaaa taaattagtg cttggtatta gtcttctgtt tttagtcatt
      301 agtatcactg ctggttgtgg catgggtaaa gaagcggaaa taaagaaaag
//
LOCUS       DANRDI010000371          336 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.379, whole genome shotgun sequence.
ACCESSION   DANRDI010000371 DANRDI010000000
VERSION     DANRDI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.379"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..178
                     /locus_tag="PZ377_002972"
     CDS             <1..178
                     /locus_tag="PZ377_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDM6359800.1"
                     /translation="KLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVE
                     KIKDYVKEQDEAEL"
     gene            189..>336
                     /locus_tag="PZ377_002973"
     CDS             189..>336
                     /locus_tag="PZ377_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDM6359801.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
BASE COUNT          132 a           33 c           75 g           96 t
ORIGIN      
        1 caaattgaat gtatcttttg attatattga ttgggtgaat tcagaatttg gaccaatggg
       61 aagagaacat tattatatgt ataaaaaatt tggaatttgg cctgaaaaag aatatgccat
      121 aaattgggtt gaaaaaataa aagattatgt taaagagcaa gatgaagctg aactataggg
      181 gagataatat gactttcgaa ggaaaactaa gtcaaatgta caacgagatt gcgaatgaga
      241 ttagtgggat gataccggta gaatgggagc aagtatttac aatagcttat gtaactgatc
      301 aagctggaga agtcatcttt aattatacta aaccag
//
LOCUS       DANRDI010000372          332 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.380, whole genome shotgun sequence.
ACCESSION   DANRDI010000372 DANRDI010000000
VERSION     DANRDI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.380"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>332
                     /gene="lpl2"
                     /locus_tag="PZ377_002974"
     CDS             <1..>332
                     /gene="lpl2"
                     /locus_tag="PZ377_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HDM6359802.1"
                     /translation="IYTDFAKSNKPGVLDNEGMILNLDRNTRTAKGYYFVDTIYDNHE
                     NSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFKFFGQYADFKSLKSYNNGDVSINS
                     NVPSYDAK"
BASE COUNT          139 a           35 c           59 g           99 t
ORIGIN      
        1 gatatataca gatttcgcca aaagtaataa accgggtgta ttagataatg aaggtatgat
       61 tttaaatttg gatagaaata cacgtacggc caagggatat tattttgtag atactatata
      121 tgacaatcat gaaaactctt atagtaaaaa ttatagagtt gagatgaaaa acaataaaat
      181 tattttatta gacaaggtgg aagatcaaaa acttaaagaa agaatagaaa actttaaatt
      241 tttcggacaa tatgccgatt tcaagagttt gaaaagttac aacaatggcg acgtttcaat
      301 taatagtaat gttccaagtt atgacgcgaa at
//
LOCUS       DANRDI010000373          330 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.381, whole genome shotgun sequence.
ACCESSION   DANRDI010000373 DANRDI010000000
VERSION     DANRDI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.381"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(45..143)
                     /locus_tag="PZ377_002975"
                     /pseudo
     CDS             complement(45..143)
                     /locus_tag="PZ377_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
     gene            complement(119..241)
                     /locus_tag="PZ377_002976"
     CDS             complement(119..241)
                     /locus_tag="PZ377_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359803.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
BASE COUNT          102 a           60 c           44 g          124 t
ORIGIN      
        1 acaattgaat tcggtttact gttaaataat aattcggttg cttgttaccc tggcacaaaa
       61 gatacactat cttgattgac atatactttt gtaaatttac catcttttaa caaataagct
      121 acttcattgc tatcattgtt ttccatgaga tatacctccg tttatagtct gtcgactgat
      181 attccatact tgcttttaaa atgtcatcag cacgttttgg gcaataaaaa ataaccttca
      241 catttgaagg ttaaaatttt taaagtaaac tttctttatc cccatgctac gagttcagat
      301 tcaggaaata gccctttact agtatttatg
//
LOCUS       DANRDI010000374          329 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.382, whole genome shotgun sequence.
ACCESSION   DANRDI010000374 DANRDI010000000
VERSION     DANRDI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.382"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>329
                     /locus_tag="PZ377_002977"
     CDS             <1..>329
                     /locus_tag="PZ377_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDM6359804.1"
                     /translation="YRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKT
                     TKGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQY
                     GNFKDLS"
BASE COUNT          152 a           35 c           60 g           82 t
ORIGIN      
        1 tatcgtgatg acgaatttga taaaaatgat aaaggtacat ggattattgg ttctgaaatg
       61 gcaactcaaa ataaggggga agctctgaaa gttaaaggta tggtcttgta tatgaataga
      121 aataccaaaa caacaaaagg atattattat gtaaatgcaa taaagaatga taaagacgga
      181 agaccccaag agaatgaaaa aaggtaccca gttaaaatgg tcgataataa aatcattcca
      241 acaaaagaaa ttaaagataa aaacataaaa aaagaaatcg aaaactttaa gttctttgtt
      301 caatatggaa actttaaaga tttgtcgaa
//
LOCUS       DANRDI010000375          320 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.383, whole genome shotgun sequence.
ACCESSION   DANRDI010000375 DANRDI010000000
VERSION     DANRDI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.383"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>320)
                     /gene="lpl5"
                     /locus_tag="PZ377_002978"
     CDS             complement(<1..>320)
                     /gene="lpl5"
                     /locus_tag="PZ377_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HDM6359805.1"
                     /translation="SLLILSIFVTSCDGDNKITGDSKETQIKKSFSKTLDMYPIKNLE
                     DLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDRNTRTAKGYYFV
                     KKFY"
BASE COUNT           94 a           69 c           35 g          122 t
ORIGIN      
        1 tcataaaatt tcttaacaaa ataatatccc ttagccgttc gagtatttct atccagattt
       61 aaaaccatac cttcatcatc caattcgtct gatttattgc ctttagcaaa atctgtatat
      121 atagtccaca ttcctttgtc gcccttttta aattcgccat cacggtatcc ttctttatca
      181 tataagtcct cgagattctt aattggatac atatctaacg ttttcgaaaa gctctttttg
      241 atttgtgttt cttttgaatc tccagtgatc ttattatcac catcgcaaga tgttacaaaa
      301 atgcttaaaa tcaacagact
//
LOCUS       DANRDI010000376          319 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.384, whole genome shotgun sequence.
ACCESSION   DANRDI010000376 DANRDI010000000
VERSION     DANRDI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.384"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            228..>319
                     /locus_tag="PZ377_002979"
     CDS             228..>319
                     /locus_tag="PZ377_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDM6359806.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSC"
BASE COUNT          126 a           35 c           45 g          113 t
ORIGIN      
        1 ttaaagagct agaagaataa actatcttaa tgtaagacta aacaataaag ctttgtttag
       61 tctttttagc gtttaagtaa aaagcaatag ataccgtaaa gttgatgctc atcaaataat
      121 aatataaaga taattttagg tttttaaact tttaatcgaa aagaatctaa gtttatagct
      181 ttaaatacgt aatatgctat ttaaaaaata atgtatagga gatagatatg actactaaag
      241 aaaatattga tactcttcga aagccaggcg cacaagcctt aagtttaata tcattgtttt
      301 tgatactttt ttcatgttt
//
LOCUS       DANRDI010000377          315 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.385, whole genome shotgun sequence.
ACCESSION   DANRDI010000377 DANRDI010000000
VERSION     DANRDI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.385"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(124..>315)
                     /locus_tag="PZ377_002980"
     CDS             complement(124..>315)
                     /locus_tag="PZ377_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDM6359807.1"
                     /translation="NVKQLRKRYDIPTNKAPKLLLKGTGNLKGSSVGYKDIEFTFVEK
                     KEENIYFSDGLIFKPSEDK"
BASE COUNT           94 a           61 c           28 g          132 t
ORIGIN      
        1 actaatgact aatatcaaaa aactaatata caataccaat ttgtttaatc gtttcataat
       61 ttcacaatcc tattcttctt attatctttc ctggattgat ttcatatttt gatcgagtca
      121 tgattattta tcctcacttg gtttaaaaat taacccatca ctaaagtaaa tgttctcttc
      181 ttttttctct acaaacgtaa attcaatgtc tttatatcca actgatgaac cttttaaatt
      241 ccctgtaccc ttcaacaaca acttcggtgc tttatttgtt ggtatgtcat atcttttacg
      301 taattgcttt acatt
//
LOCUS       DANRDI010000378          301 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.386, whole genome shotgun sequence.
ACCESSION   DANRDI010000378 DANRDI010000000
VERSION     DANRDI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.386"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..158
                     /locus_tag="PZ377_002981"
     CDS             <1..158
                     /locus_tag="PZ377_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDM6359808.1"
                     /translation="PVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPA
                     KVDEDDE"
     gene            177..>301
                     /locus_tag="PZ377_002982"
     CDS             177..>301
                     /locus_tag="PZ377_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDM6359809.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKS"
BASE COUNT          107 a           27 c           72 g           95 t
ORIGIN      
        1 caccggtatt aaaaatgcat attgatggta atttgaaagg aagttctgtg ggttataaaa
       61 agttggaaat tgacttttca aaaggtggaa aaagcgattt gtcagtaata gattctttga
      121 atttccagcc ggcgaaggta gatgaagatg atgaatgatg aggatggtgt gtaacaatga
      181 agtctataaa aaggattgga ttgtgcatta gtttgttgat tttaatcatc tttgttacat
      241 cttgtgatgg tgataataag atcattggag attcaaaaga agaacaaatc aaaaagagct
      301 t
//
LOCUS       DANRDI010000379          291 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.387, whole genome shotgun sequence.
ACCESSION   DANRDI010000379 DANRDI010000000
VERSION     DANRDI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.387"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..218
                     /locus_tag="PZ377_002983"
     CDS             <1..218
                     /locus_tag="PZ377_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacitracin ABC transporter permease"
                     /protein_id="HDM6359810.1"
                     /translation="KGLLLKITFNFGLPLLIAILHAVFAAIAFMKLMGNISFMPVIVV
                     IVVYTLIYITFALIAFVHSNKLIKKTI"
BASE COUNT           94 a           41 c           38 g          118 t
ORIGIN      
        1 ttaagggatt attattaaaa attactttca acttcggttt accattattg attgctatat
       61 tacatgcagt attcgccgca atagcattta tgaaattgat gggtaatatt tccttcatgc
      121 cggttattgt agtaattgtt gtatacaccc tcatttacat tacttttgct ttaattgctt
      181 ttgtacattc aaacaaattg attaagaaaa ccatttaaca cttacgatta aaagatgctt
      241 cattagatat tttgatttta agcaatgcag tataactatt aattgattta a
//
LOCUS       DANRDI010000380          284 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.388, whole genome shotgun sequence.
ACCESSION   DANRDI010000380 DANRDI010000000
VERSION     DANRDI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.388"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
BASE COUNT          104 a           45 c           43 g           92 t
ORIGIN      
        1 gtgggacgac gaaataaatt ttacgaaaat atcatttctg tcccactccc taataactga
       61 gttttaataa agtcttttaa cccacattaa atattatttt gcaattgcaa tgaataacaa
      121 gaaaaatctg ggacattaat cgatcaaatg ctcccttcaa agtagacatt gaataaatga
      181 aggctttgaa gggagcattt cactttgtac ttggctcaac aattttatat agacagtagt
      241 taattgaatg aaaataagct tgtaacaagt tttcaattct agtc
//
LOCUS       DANRDI010000381          278 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.389, whole genome shotgun sequence.
ACCESSION   DANRDI010000381 DANRDI010000000
VERSION     DANRDI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.389"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(24..>278)
                     /locus_tag="PZ377_002984"
     rRNA            complement(24..>278)
                     /locus_tag="PZ377_002984"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           76 c           63 g           70 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaact
//
LOCUS       DANRDI010000382          265 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.390, whole genome shotgun sequence.
ACCESSION   DANRDI010000382 DANRDI010000000
VERSION     DANRDI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.390"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
BASE COUNT           92 a           48 c           39 g           86 t
ORIGIN      
        1 ttacgtctat tgaatcatac agctttatta tagttagcgt atttgacctt tcacattaaa
       61 ccatgtttaa taatcattga atcattatta agtaaattaa ggaatctata atgttcgtta
      121 aataaaactg atcccgttgt gcttcacacc cgatagatag ggatttacag ataaattcag
      181 gtctcttcca cgtcatattt ggacccatcg aaaattcggg ttctcaaatc atcgaacata
      241 acaaaagaag ctaagcaaca tgtag
//
LOCUS       DANRDI010000383          260 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.391, whole genome shotgun sequence.
ACCESSION   DANRDI010000383 DANRDI010000000
VERSION     DANRDI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.391"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>260
                     /locus_tag="PZ377_002985"
     CDS             <1..>260
                     /locus_tag="PZ377_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HDM6359811.1"
                     /translation="NYDVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKY
                     EQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKDKPKYEHGG"
BASE COUNT          108 a           35 c           59 g           58 t
ORIGIN      
        1 aattatgacg tgattgaaga aatcgaagaa aatagccacg ttgatattaa gagtgaatta
       61 ggttatgaag gtggccaaaa tagcggtaac cagtcattcg aggaagacac agaagaagac
      121 aaacctaaat atgaacaagg tggcaatatc gtagatatcg attttgatag tgtacctcaa
      181 attcatggtc aaaataaagg taatcagtca ttcgaggaag atacagaaaa agacaaacct
      241 aagtatgaac atggcggtaa
//
LOCUS       DANRDI010000384          260 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.392, whole genome shotgun sequence.
ACCESSION   DANRDI010000384 DANRDI010000000
VERSION     DANRDI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.392"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>260
                     /locus_tag="PZ377_002986"
     CDS             <1..>260
                     /locus_tag="PZ377_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HDM6359812.1"
                     /translation="NYGVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKY
                     EQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQGG"
BASE COUNT          108 a           34 c           61 g           57 t
ORIGIN      
        1 aattatggcg tgattgaaga aatcgaagaa aatagccacg tggatattaa gagtgaatta
       61 ggttacgaag gtggccaaaa tagcggtaat cagtcatttg aggaagacac agaagaagat
      121 aaaccgaaat atgaacaagg tggcaatatc gtagatatcg atttcgatag tgtacctcaa
      181 attcatggtc aaaataatgg taaccaatca ttcgaagaag atacagagaa agacaaacct
      241 aagtatgaac aaggtggtaa
//
LOCUS       DANRDI010000385          253 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.393, whole genome shotgun sequence.
ACCESSION   DANRDI010000385 DANRDI010000000
VERSION     DANRDI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.393"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            86..253
                     /locus_tag="PZ377_002987"
                     /pseudo
     CDS             86..253
                     /locus_tag="PZ377_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT           87 a           43 c           46 g           77 t
ORIGIN      
        1 cctattattt aaaacgtcgt gaagaaaaag gtaaataaat aagccttatg tgctaaactc
       61 aatatgtatt tcctaccaaa gaaaacataa ggagtttgga catcttgaag gagatactat
      121 tgttggtcgt cgtcatggta gtgcaatcat tactttggta gaaagagtat ccaagatgat
      181 tatcacctta cgacccttag gtcgtaaggc aactgatata gaaaacgctc tacactcaat
      241 gttttcatct tta
//
LOCUS       DANRDI010000386          247 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.394, whole genome shotgun sequence.
ACCESSION   DANRDI010000386 DANRDI010000000
VERSION     DANRDI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.394"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            6..>247
                     /locus_tag="PZ377_002988"
     CDS             6..>247
                     /locus_tag="PZ377_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359813.1"
                     /translation="MLLVVALPIYTVASYQNKEVHQGTITDKYNKRQDKEDKFYIVLD
                     DKQVIENSDLFFKGKFDSADIQARLKVGDKVKVKTIG"
BASE COUNT          101 a           37 c           47 g           62 t
ORIGIN      
        1 ccgcattgct acttgttgtc gcattgccta tctatacagt ggctagttac caaaacaaag
       61 aagtacatca agggacaatt acagataaat ataacaaaag acaagataaa gaggacaaat
      121 tctatattgt attagatgat aaacaagtca tcgaaaactc agacttattc ttcaaaggaa
      181 agtttgatag cgcagacata caagctaggt taaaagtagg tgataaagta aaagttaaga
      241 cgattgg
//
LOCUS       DANRDI010000387          247 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.395, whole genome shotgun sequence.
ACCESSION   DANRDI010000387 DANRDI010000000
VERSION     DANRDI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.395"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>247
                     /locus_tag="PZ377_002989"
     CDS             <1..>247
                     /locus_tag="PZ377_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359814.1"
                     /translation="ALLLVITLPIYTVASYQHKELHQGTITDKYNKRQDKEDKFYIVL
                     DNKQVIENSDLLFKKKFDSADIQARLKVGDKVEVKTIG"
BASE COUNT          104 a           38 c           41 g           64 t
ORIGIN      
        1 tcgcattgct acttgttatc acattgccta tctatacagt ggctagttac caacacaaag
       61 aattacatca aggaactatt acagataaat ataacaagag acaagataaa gaagacaagt
      121 tctatattgt attagacaac aaacaagtca ttgaaaattc cgacttatta ttcaaaaaga
      181 aatttgatag cgcagatata caagctaggt taaaagtagg cgataaggta gaagttaaaa
      241 caatcgg
//
LOCUS       DANRDI010000388          245 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.396, whole genome shotgun sequence.
ACCESSION   DANRDI010000388 DANRDI010000000
VERSION     DANRDI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.396"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>245)
                     /locus_tag="PZ377_002990"
     CDS             complement(<1..>245)
                     /locus_tag="PZ377_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359815.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGK"
BASE COUNT           45 a           58 c           62 g           80 t
ORIGIN      
        1 tgttttcctg catccgagtc tgaatcgctg tctgaatcac tgtctgagtc tgagtcgcta
       61 tcagagtctg agtcgctgtc cgaatctgag tcgctatctg agtctgagtc gctgtctgaa
      121 tctgaatcac tgtctgagtc tgagtcgcta tctgagtctg aatcgctgtc tgaatctgag
      181 tcgctatctg agtccgaatc gctatctgaa tctgagtcgc tgtctgagtc tgagtcgcta
      241 tctga
//
LOCUS       DANRDI010000389          243 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.397, whole genome shotgun sequence.
ACCESSION   DANRDI010000389 DANRDI010000000
VERSION     DANRDI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.397"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
BASE COUNT           80 a           45 c           29 g           89 t
ORIGIN      
        1 taatgctata ctttcaattt cttaacatgg ctatctcatc agatgaatag tatttatagc
       61 catgcaaatc aatgatggca catatttctt aatgccattt gatactgtct caagggattc
      121 ctcgttatac tgtaacaatt ggtcacaatc tttaaaatat aacttttatt tgaacttatt
      181 aagtaaatta agactacctt gagccttccc ctgtaataac aaccatcaat gttctaattg
      241 ata
//
LOCUS       DANRDI010000390          239 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.398, whole genome shotgun sequence.
ACCESSION   DANRDI010000390 DANRDI010000000
VERSION     DANRDI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.398"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            6..>239
                     /locus_tag="PZ377_002991"
     CDS             6..>239
                     /locus_tag="PZ377_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDM6359816.1"
                     /translation="MKHFAQLTLPIETAVRIPQNDISRYVNEIVKKIADSEFDEFRHH
                     RGATSYHLKMMLKITSYSYTQSEFSGRRIEKLLH"
BASE COUNT           93 a           44 c           31 g           71 t
ORIGIN      
        1 aaaaaataaa acactttgcc caacttacac taccaataga aactgctgtt agaattcctc
       61 aaaatgatat ttcgcgatat gttaatgaaa ttgttaaaaa gatagctgat agcgaattcg
      121 atgaattcag acatcatcgt ggcgcaacat cctatcatct aaaaatgatg ttaaaaatca
      181 cctcatattc atatactcaa tctgaatttt ctggccgtag aatagaaaaa ttacttcat
//
LOCUS       DANRDI010000391          239 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.399, whole genome shotgun sequence.
ACCESSION   DANRDI010000391 DANRDI010000000
VERSION     DANRDI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.399"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>239
                     /locus_tag="PZ377_002992"
     CDS             <1..>239
                     /locus_tag="PZ377_002992"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HDM6359817.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGAT"
BASE COUNT           82 a           30 c           67 g           60 t
ORIGIN      
        1 gtaaaagtga ttattgagac ggtattgttg gaccatgacg aaattgtaaa agcgagtgaa
       61 ttaacaaaaa cggctggtgc ggacttcgtt aaaacttcaa caggttttgc aggtggcggt
      121 gcgactgcag aagacgttaa attaatgaaa gatacagtag gtgctgatgt agaagtaaaa
      181 gcatcaggtg gcgtacgtaa tttagaagat ttcaataaaa tggttgaagc aggtgcgac
//
LOCUS       DANRDI010000392          238 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.400, whole genome shotgun sequence.
ACCESSION   DANRDI010000392 DANRDI010000000
VERSION     DANRDI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.400"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
BASE COUNT           83 a           43 c           28 g           84 t
ORIGIN      
        1 ttcgatttaa aagatattag acataaaatc taaaaacagc agtaagatga tttatgatta
       61 aaaactatct tactgctgtt cactttttat aatacttctg aatgtcttca cttatacttc
      121 tagtcacaga tttaaataat caaaagtgca cattattaaa atatcaattt cacactcaat
      181 gcggctcatc gcattcattt cttgtctagc aacgttctac tctagcggaa cgtaagtt
//
LOCUS       DANRDI010000393          237 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.401, whole genome shotgun sequence.
ACCESSION   DANRDI010000393 DANRDI010000000
VERSION     DANRDI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.401"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            20..93
                     /locus_tag="PZ377_002993"
     tRNA            20..93
                     /locus_tag="PZ377_002993"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54..56,aa:Met,seq:cat)
     gene            122..195
                     /locus_tag="PZ377_002994"
     tRNA            122..195
                     /locus_tag="PZ377_002994"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Ile,seq:cat)
BASE COUNT           57 a           52 c           61 g           67 t
ORIGIN      
        1 ttatttacaa actatataag gcggtgtagc tcagctggct agagcgtacg gttcataccc
       61 gtgaggtcgg gggttcgatc ccctccaccg ccactattta ttagttgtaa aattatattt
      121 aggaccttta gctcagttgg ttagagctaa cggctcataa ccgttcggtc gcaggttcga
      181 gtcctgcaag gtccatataa ttttggagga atacccaagt ccggctgaag ggatcgg
//
LOCUS       DANRDI010000394          231 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.402, whole genome shotgun sequence.
ACCESSION   DANRDI010000394 DANRDI010000000
VERSION     DANRDI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.402"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
BASE COUNT           66 a           38 c           36 g           91 t
ORIGIN      
        1 gtttagatca tgttttgtat ttcgacatta acgttaaagg tgaaaagaat aacgatagtc
       61 cttatccatc ttaaaatcca tagtgcttat agttatttgc tatttgttga cagttatcac
      121 tggcaagtcc taccttgtca gcttggtaac tgtattgatt atttacttca tttactaatc
      181 gattccattc gtctcttggt atatcttcaa gaagtaagag aatcatcttt a
//
LOCUS       DANRDI010000395          227 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.403, whole genome shotgun sequence.
ACCESSION   DANRDI010000395 DANRDI010000000
VERSION     DANRDI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.403"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>227
                     /locus_tag="PZ377_002995"
     CDS             <1..>227
                     /locus_tag="PZ377_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDM6359818.1"
                     /translation="TLLTINDDIPDRKTGLELAEKYGIDGIMIGRGIFHNPFTFEKEP
                     REHTSKELLDLLRLHLSLFNKYEKDEIRQFK"
BASE COUNT           90 a           36 c           40 g           61 t
ORIGIN      
        1 acattgttaa caattaacga tgatattccc gatagaaaaa caggacttga acttgcagaa
       61 aaatatggta ttgatggcat catgattggt agaggcattt tccacaatcc attcactttt
      121 gaaaaagaac cacgcgaaca cacaagcaaa gaactattag atttgttgag attgcatcta
      181 tcattgttta acaaatatga gaaagatgaa atacgacaat tcaagag
//
LOCUS       DANRDI010000396          227 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.404, whole genome shotgun sequence.
ACCESSION   DANRDI010000396 DANRDI010000000
VERSION     DANRDI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.404"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>227
                     /locus_tag="PZ377_002996"
     CDS             <1..>227
                     /locus_tag="PZ377_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDM6359819.1"
                     /translation="TLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPFAFEKEP
                     REHTSKELLDLLRLHLSLFNKYEKDEIRQFK"
BASE COUNT           88 a           37 c           43 g           59 t
ORIGIN      
        1 acattgttaa caattaacgg tgatattccc gatagaaaaa caggacttga actggcagaa
       61 aaatatggca ttgatggcgt catgattggt agaggcattt tccacaatcc attcgctttt
      121 gaaaaagaac cacgcgaaca cacaagcaag gaactattag atctattgag attgcattta
      181 tcattgttta acaaatatga aaaagatgaa atacgacaat tcaagag
//
LOCUS       DANRDI010000397          224 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.406, whole genome shotgun sequence.
ACCESSION   DANRDI010000397 DANRDI010000000
VERSION     DANRDI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.406"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>224
                     /locus_tag="PZ377_002997"
     CDS             <1..>224
                     /locus_tag="PZ377_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDM6359820.1"
                     /translation="VISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITVVFAL
                     CNLYYFAAVQNHYDLFNYWLMGSISIIISY"
BASE COUNT           75 a           23 c           34 g           92 t
ORIGIN      
        1 gtaattagtt tacttcaatg gattagattt atagatttcg aaaaagaaag tgcacagaaa
       61 tataaaaaaa tatatgctcg atttttagtt gttataaatg tgttaactac tatcactgta
      121 gtatttgcac tgtgtaatct ttattatttt gcagctgtac aaaaccatta tgatttattt
      181 aattattggt tgatgggttc gatttcaatc ataattagct attt
//
LOCUS       DANRDI010000398          223 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.407, whole genome shotgun sequence.
ACCESSION   DANRDI010000398 DANRDI010000000
VERSION     DANRDI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.407"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..174
                     /locus_tag="PZ377_002998"
     CDS             <1..174
                     /locus_tag="PZ377_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDM6359821.1"
                     /translation="KRYDIPTNKAPKLLLKGTGNLKGSSVGYKKIEFTFLENKNENIY
                     FTDSLHLEPSEDK"
BASE COUNT           96 a           29 c           36 g           62 t
ORIGIN      
        1 aaaagatatg acataccaac aaataaagcg ccgaagctgt tgttgaaagg tacagggaat
       61 ttaaaaggtt catcagttgg atataaaaaa attgaattta cttttttaga gaataaaaat
      121 gaaaatattt actttactga tagtctacat cttgaaccga gcgaggataa ataatcatct
      181 tttaaccaga atatgaaatc aatctaggac aacctaataa aag
//
LOCUS       DANRDI010000399          215 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.408, whole genome shotgun sequence.
ACCESSION   DANRDI010000399 DANRDI010000000
VERSION     DANRDI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.408"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(<1..>215)
                     /locus_tag="PZ377_002999"
     CDS             complement(<1..>215)
                     /locus_tag="PZ377_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359822.1"
                     /translation="SESDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDS"
BASE COUNT           40 a           50 c           53 g           72 t
ORIGIN      
        1 tcgctgtctg agtctgagtc gctgtctgag tctgaatcgc tatctgaatc tgagtcgctg
       61 tctgagtctg agtcgctgtc tgaatctgag tcgctgtctg aatctgaatc gctgtctgag
      121 tctgaatcgc tatctgagtc tgaatcgcta tctgagtctg aatcactgtc tgagtccgag
      181 tcactgtctg aatctgactc actatctgat tctga
//
LOCUS       DANRDI010000400          205 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.409, whole genome shotgun sequence.
ACCESSION   DANRDI010000400 DANRDI010000000
VERSION     DANRDI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.409"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
BASE COUNT           78 a           23 c           30 g           74 t
ORIGIN      
        1 aacttacgtt ccgctagagt agaactggaa atgataattt aataatgtac actttcgatt
       61 gtctaagtat gtacaacttt aattttgtgt ttatataaat ttaaaatgat atcatcgaaa
      121 acaaaatatt gtataaatag agaagagcag taagacggta tctaattgaa aatgatctta
      181 ctgctctttt atatacttta ttgaa
//
LOCUS       DANRDI010000401          203 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.410, whole genome shotgun sequence.
ACCESSION   DANRDI010000401 DANRDI010000000
VERSION     DANRDI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.410"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            3..140
                     /locus_tag="PZ377_003000"
     CDS             3..140
                     /locus_tag="PZ377_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359823.1"
                     /translation="MINTTITYGYTTEGTWVNLLFKSLSLSMIIVFMFYYIRFVIEKK
                     R"
BASE COUNT           74 a           26 c           36 g           67 t
ORIGIN      
        1 tcatcattaa cacaacgatt acatatggat atacgactga gggaacgtgg gtcaatctat
       61 tatttaaatc tttatcgctc agcatgatta tcgttttcat gttctactat attcgttttg
      121 ttattgagaa aaagcgttaa atcaaagcta tggggatagt atttgaaaag aataaataaa
      181 agggacaatg taaatcaatg taa
//
LOCUS       DANRDI010000402          203 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.411, whole genome shotgun sequence.
ACCESSION   DANRDI010000402 DANRDI010000000
VERSION     DANRDI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.411"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            52..>203
                     /gene="tnpA"
                     /locus_tag="PZ377_003001"
     CDS             52..>203
                     /gene="tnpA"
                     /locus_tag="PZ377_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDM6359824.1"
                     /translation="MVFAPKYRRQVIYGKIKKDIGIILRQLCERKGVEIIEAEACKDH
                     IHMLVS"
BASE COUNT           85 a           22 c           39 g           57 t
ORIGIN      
        1 tcatctgaca caaacagttt agcacataca aaatggaatt gtaagtatca tattgtcttt
       61 gcacctaaat acagaagaca agtgatatat ggaaaaataa aaaaagatat agggattata
      121 ttgcgtcaat tatgtgaaag aaaaggtgta gagataattg aagcagaggc atgtaaagat
      181 catatccata tgttagttag tat
//
LOCUS       DANRDI010000403          202 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.denovo.412, whole genome shotgun sequence.
ACCESSION   DANRDI010000403 DANRDI010000000
VERSION     DANRDI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.denovo.412"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..>202
                     /locus_tag="PZ377_003002"
     rRNA            <1..>202
                     /locus_tag="PZ377_003002"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           45 c           59 g           50 t
ORIGIN      
        1 gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa
       61 cgagcgcaac ccttaagctt agttgccatc attaagttgg gcactctaag ttgactgccg
      121 gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgatttgggc
      181 tacacacgtg ctacaatgga ca
//
LOCUS       DANRDI010000404         6660 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.guided.138, whole genome shotgun sequence.
ACCESSION   DANRDI010000404 DANRDI010000000
VERSION     DANRDI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.guided.138"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            485..1951
                     /gene="msr(A)"
                     /locus_tag="PZ377_003003"
     CDS             485..1951
                     /gene="msr(A)"
                     /locus_tag="PZ377_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HDM6359825.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2050..2949
                     /locus_tag="PZ377_003004"
     CDS             2050..2949
                     /locus_tag="PZ377_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HDM6359826.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3435..4043
                     /locus_tag="PZ377_003005"
     CDS             3435..4043
                     /locus_tag="PZ377_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDM6359827.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4289..4627
                     /locus_tag="PZ377_003006"
     CDS             4289..4627
                     /locus_tag="PZ377_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359828.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4632..4817
                     /locus_tag="PZ377_003007"
     CDS             4632..4817
                     /locus_tag="PZ377_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDM6359829.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5044..5787)
                     /locus_tag="PZ377_003008"
     CDS             complement(5044..5787)
                     /locus_tag="PZ377_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDM6359830.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            6213..>6660
                     /locus_tag="PZ377_003009"
     CDS             6213..>6660
                     /locus_tag="PZ377_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDM6359831.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENR"
BASE COUNT         2533 a          951 c         1026 g         2150 t
ORIGIN      
        1 aaatagttca tacagaagac tcctttttgt taaaattata ttataaattc aactttgcaa
       61 cagaaccgta ttttatgagt acactctaaa ttaaggagat gtataatcaa tctaaaattg
      121 tatttttata cttttgttat ataatctttc tacttagaga tatattgtaa aaaaaccaac
      181 aaaatgttat attaacatta ctattttatc gatgaaggag gattaaaatg acagcttcta
      241 tgagactcaa ataaaccttt ttgaatacaa aactaaatta attcaaaaag gcttgtccgc
      301 aatacacact cataggatga cttatgtcat tttttatcct tcgataggaa taataaacgc
      361 tttcaaatac taaaacatca taataaattt ttaagtgttt gaaagtgtaa tgtgaatgag
      421 acgggagtag cctgtctcat ttttttgcgt acacgtcctt taaatataca aggagtgtat
      481 aaatatggaa caatatacaa ttaaatttaa ccaaatcaat cataaattga cagatttacg
      541 atcacttaac atcggtcatc tttacgctta ccaatttgaa aaaatagcac ttattggggg
      601 taatggtact ggcaaaacca cattactaaa tatgattgct caaaaaacaa aaccagaatc
      661 tggaacagtt gaaacggttg gcgaaattca atattttgaa cagcttaaca tggatgtgga
      721 aaatgatttt aacacgttag acggtagttt aatgagtgaa ctccatatac ctatgcatac
      781 aaccgacagt atgagtggtg gtgaaaaagc aaaatataaa ttagctaatg tcatatcaaa
      841 ttatagtccg atattacttt tagatgaacc tacaaatcac ttggataaaa ttggtaaaga
      901 ttatctgaaa aatattttaa aatattacta tggtacttta attatagtaa gtcatgatag
      961 agcacttata gaccaaattg ctgacacaat ttgggatata caagaagatg gcacaataag
     1021 agtgtttaaa ggtaattaca cacagtatca aaatcaatat gaacaagaac agttagaaca
     1081 acaacgtcaa tatgaacagt atataagtga aaaacaaaga ttgtcccaag ccagtaaagc
     1141 taaacgaaat caagcgcaac aaatggcaca agcatcatca aaacaaaaaa acaaaagtat
     1201 agcaccagat cgtttaagtg catcaaaaca aaaaggcacg gttgagaagg ctgctcaaaa
     1261 acaagctaag catattgaaa aaagaatgga acatttggaa gaagttgaaa aaccacaaag
     1321 ttatcatgaa ttcaatttcc cacaaaataa aatttatgat atccataata attatccaat
     1381 cattgcacaa aatctaacat tggttaaagg aagtcaaaaa ctgctaacac aagtacgatt
     1441 ccaaatacca tatggcaaaa atatagcgct cgtaggtgca aatggtgtag gtaagacaac
     1501 tttacttgaa gctatttacc accaaataga gggaattgat tgttctccta aagtgcaaat
     1561 ggcatactat cgtcaacttg cttatgaaga catgcgtgac gtttcattat tgcaatattt
     1621 aatggatgaa acggattcat cagaatcatt cagtagagct attttaaata acttgggttt
     1681 aaatgaagca cttgatcgtt cttgtaatgt tttgagtggt ggggaaagaa cgaaattatc
     1741 gttagcagta ttattttcaa cgaaagcgaa tatgttaatt ttggatgaac caactaattt
     1801 tttagatatt aaaacattag aagcattaga aatgtttatg aataaatatc ctggaatcat
     1861 tttgtttaca tcacatgata caaggtttgt taaacatgta tcagataaaa aatgggaatt
     1921 aacaggacaa tctcttcatg atataactta aaaactatac actaatttat attgaatatt
     1981 aggttcattt atatcaatat aaaaaatacg ttcacaagaa aaatataata gaaataagga
     2041 tccaataaaa tgactcgaca taatgaaatt attaaatgtg cagaaaaata tcaattacac
     2101 atccaacctc aaacaatctc attgaatgaa tcgggacttg attttcaagt tgcatttgga
     2161 aaagataaac atggagtaga atgggttttg agactaccaa gaagacctga cgtttataaa
     2221 cgaacaaaac ccgaaaaaca aacggtagac ttcttacaga agaatgtttc atttgaaata
     2281 ccgaagtgga aagtacatgc aaaagacctt attgcttacc caaaacttac aggtaaaccc
     2341 gcagccacaa tagatccaga aatacaaaat tatgtatggg aaattgaaca caaaccatta
     2401 ccagaaaact ttattaacac attagctgaa acactcgtag atttacacaa cataccagaa
     2461 gaaaacatta acgttcagca tataaatatc aaaaccatac aagaaataaa aaatgacttt
     2521 caaagaagaa tgaataaagt taaagaaact tatggcgtat cagatgaatt atggaacaga
     2581 tggaaacaat ggttagaaaa cgacgaacta tggcctcgac atgcgaccat gatacatggg
     2641 gacttacatc caggacatat aatggtagat aaccaagcaa acgtcacagg tctcatagac
     2701 tggactgaag caacccactc cgacccatca atggacttta taggacacca tcgtgtattc
     2761 gacgacgaag gattagagca acttataaca gcatacggta aagctggagg tgaaatatgg
     2821 ccacgaatga aagagcatat aatagaactc aatgcagtat tcccaatgtt tatcgctgag
     2881 tttgctatgg aatcaggaga atcggcgtat gaaacgatgg cattgaaaga gttaggtatg
     2941 aaagagtaga acttatatta aaagaggccc attttaaaga cgaaggcaat cctgatgaaa
     3001 ttttaaaact gcgtttaatt agtgatggtc tttggttttc tataatgtat taaatatctg
     3061 gacatggaac aaatgcaaca aatggaaaga atcgttttcg aaatttctaa ctccttaaat
     3121 caggaggata aataaacggt cattttcatt gaaagcactt atataaattt catcaatttt
     3181 aatacctacc gtaaatacat ttcataagta ttaaacagat gattaaaatt tttagttaat
     3241 ttaaatattt atgatagaga tgaccttcaa atgaacggtt ctgttacaaa gaaaaaatct
     3301 atatttattt ttagtaagtt ttaattatag atatgtacac tttgagtaca ccctaaccat
     3361 acaaaaagaa ttgttgaaat tataacaatt aaaacgtatg attagggtgt atatttatta
     3421 tggagtgaag atgaatgatt ataggttatg cgagagtatc atcgatagat caaaatttag
     3481 aaagacaatt ggataattta aagacgtttg gtgtggagaa aatctttaca gagaaacagt
     3541 cgggaaagtc agtggaaaat agacctgtat ttcaagaagc actcaatttt gtcagaatgg
     3601 gagatcgatt cgttgtggaa tctattgatc gtttaggacg taactatgat gaaattattg
     3661 aaactgtgaa ttatttaaaa gaaaaagacg ttcagttaat gattaccagc ttacctatga
     3721 tgaatgaagt aattggcaat ccattactgg ataagtttat gaaagattta atcattcaaa
     3781 tattagcaat ggtttcagaa caagagagaa atgaaagtaa acgtcgacaa gcgcaaggga
     3841 ttcaagttgc gaaggaaaaa ggcgtatata aagggcgccc tttactttat tcaccaaatg
     3901 ctaaagaccc acagaaacgt gttatttatc atcgcgtggt ggaaatgtta gaagaaggca
     3961 aagcgattag taagattgcg aaagaagtta atatcacaag acaaacagtt tatagaatta
     4021 aacatgataa gggattatct tgataaattg attgtcaatg caaatgagcg tctctcacag
     4081 tgtctcaaat tccataacat attattgaat tgagatttta ataattatac aagaaaaaag
     4141 agtacgagtt cgcagctttc tatatagtaa ttgttttact aataaggtgt tttaaaatat
     4201 attataatgt aaaaagtatt tgacatttgt atcaaaaata tttacactca taaatgtcaa
     4261 atacttttta catttatgag gtgttataat gaatttaatt ccttttatta ttattttaat
     4321 tttttcttgt ttatggacgg ctataactaa caaatattta ccttcagaaa cattagataa
     4381 aaaaggacaa caagatcgat acgatgaaag acaacgtaaa atgtttattg aaatactggc
     4441 taaaagtttt atttggattg tatattgtat gttatttaca ctagtgctta aatttacagg
     4501 tttgtcacca agtattgaag gatcttggtt ttctcaatat ccagaaattt tctttataat
     4561 tgtagcacta tttcttatgc tgtttaatta ctatactaca aataaaaaat atacatctaa
     4621 aggttaaatt aatgaaaaat agaattagag aatatagaaa acaaaaagga atttctcaag
     4681 aaactttaag taaagaatta aaagtgtcta ggcaaactat taatgcaata gaaaacaata
     4741 aatatgatcc tacattgaca ttagcattta agttggcaag attgtttgat acaactgtgg
     4801 atgaattatt ttcttaacca ttcatttatc acaaaaagag agtgcgaatt cgcactctct
     4861 ttttgtgtag tagtactatt aaattttgtc tcaataacga acgtttataa cgcaatcatg
     4921 atgagagtat taatacagtt aattatttga aatactttag tttacataat cgatgattac
     4981 cagaagttct cactatataa aagacactaa aattttataa cttttagtgc ctaatcaatt
     5041 attttagcta ccaaacaaac gactccaaaa ccccttttta ggttccgttt cttcactctt
     5101 ttcttctata tcactatcat tgtcatttag attagctgga acgtcttctt tttttgcttc
     5161 ttcaccttgt gtactttcgt cgctttgact tccagatata tccttatatt gaatctctgt
     5221 ttctttagtt tcttcctgat ctctgttaat atttacagga ttagtagtat atgttttttc
     5281 ttgtgcatca acgttttgtc tagtgttagt agatgtatca aagggataat taaggtgttt
     5341 ttctttttct aattgatttt ctaatttttg aatcttttta ttactctcta atgccagaac
     5401 ttgctgattt tcaagcaatt ttgatctttc ttgcaactct tttctgtaaa attcaattgt
     5461 tgtttcatga cgactttctt gtttttcaat ttgattattt aaagtatcaa tctgattttt
     5521 aaggatttct actatttgag catcgctttt cgattcaaaa ttaatattat ccgacccttt
     5581 tttttgtttg tcttcagtag tactttcaaa accatatttc tttttatttt catttactct
     5641 ttgaatgatt ttttctatat cggtatcttc tttaataaac gaagtgtttt cctgttttat
     5701 tgtctctata ttcaatcgct taatattatt aaaaatcgtt tgcttactaa cccctaattc
     5761 ttcactcaac tttttcacac ttttcatatc atcaaccctt tataaaatgt ttataaaccc
     5821 ttgataaaat attataagct tataatgctt aataataaag tgattaataa ataaaaataa
     5881 acatccatca acctttttat caagggttta tgaaaggttt atgatattta tcaagggttt
     5941 ataaaccctt gataaagcta tattttccct tttataagtt tatttaaagt gctataataa
     6001 acttaatata acaaaaaccc caactactgg taatagtcgg ggtttggtaa taaaacgttt
     6061 agaaactgtt tatttatata attttcgata cttgtattat ataatgaata atatcttagt
     6121 gcaagcacct aaatcattaa ataaatgtaa agctaaataa aaacgtttta ttacctctta
     6181 ccaaactaaa atatggaaag aggttttttg tcatgcaaaa tcaatatttt acagtgcaag
     6241 aaaattacaa agaaagattt tatcaaatcc ctaaagtatt ctttaccagt gaaaattata
     6301 aaaatctaac taatgatatg aaaatagcct atgctatctt acgtgatcgc ttaaatttat
     6361 ctattaaaaa tagctgggtt gatgaagatg gtaacattta ctttgtttat tcaaatgaaa
     6421 agctaatgga aatcttaaat tgtaaaaaag aaaagttaac aaaaataaaa aaagggttag
     6481 aaaacgatgg tctactcatt caaaaacgac gtggtttaaa taaaccaaat attctatatt
     6541 taatgaaacc tattgtcaca gaacgtgata tttacaaaat agaaaaagaa gaaaacgatg
     6601 ttgaacctta cggcgagaag gaagttcgaa aatcgaacgt ccagaagttc gaaaatcgaa
//
LOCUS       DANRDI010000405         4795 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.guided.181, whole genome shotgun sequence.
ACCESSION   DANRDI010000405 DANRDI010000000
VERSION     DANRDI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.guided.181"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            complement(221..1066)
                     /gene="blaZ"
                     /locus_tag="PZ377_003010"
     CDS             complement(221..1066)
                     /gene="blaZ"
                     /locus_tag="PZ377_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDM6359832.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            1173..2930
                     /gene="blaR1"
                     /locus_tag="PZ377_003011"
     CDS             1173..2930
                     /gene="blaR1"
                     /locus_tag="PZ377_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDM6359833.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            2920..3300
                     /gene="blaI"
                     /locus_tag="PZ377_003012"
     CDS             2920..3300
                     /gene="blaI"
                     /locus_tag="PZ377_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDM6359834.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            3564..4142
                     /locus_tag="PZ377_003013"
     CDS             3564..4142
                     /locus_tag="PZ377_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDM6359835.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(4365..4550)
                     /locus_tag="PZ377_003014"
     CDS             complement(4365..4550)
                     /locus_tag="PZ377_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDM6359836.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
BASE COUNT         1843 a          603 c          682 g         1667 t
ORIGIN      
        1 ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat
       61 atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg
      121 gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa
      181 actataagat gttatcagta tttattatgc atttagaata ttaaaattcc ttcattacac
      241 tcttggcggt ttcacttatc aacttatcat ttggcttatc acttttattg tctttattcg
      301 taaaaatgac taaaacaata ggttcagatt ggcccttagg ataaacaaaa gcaacatcat
      361 ttctagaagc atatgttatt gcttgaccac ttttatcagc aaccttatag tcttttggaa
      421 caccatcttt aattaaagtg tctccgtttt tattattaaa cattaaatca agtaagaaat
      481 ttttattttt tttgcttaat tttccatttg cgataagttt atttaaagtc ttgccgaaag
      541 cagcaggcgt tgaagtatct tttttgctct ttggtgaata gtaatttaat tctatctcat
      601 atctaactgg atttgttact ttatctccta gttcttttag acgttgttta acttttttga
      661 ttccaccgat ttcttttata attttattgt ttgctgtatt atcactatat gtcattgaag
      721 cctcaataag ttcttttaaa gtgatatctt ttcctacata tttttctaaa ataggagaat
      781 aagcaactat atcatctttg ttaatatgta cttttttatt taacttatta taaggtactt
      841 gttctaacaa aatagcacta tttatcgctt ttgaagtcga agcataggca aatctcttat
      901 ctgaattaaa ttttacttcc ttaccacttt tagtatttaa agcataaaca ccaatatgag
      961 cattatattt tttttctaaa tcatttaact ctttggcatg tgaactgttt gaattacatg
     1021 cacttaaaac taaagcaatt acaattaaaa atattaactt tttcaaaata aaccctccga
     1081 tattacagtt gtaattttta taaatcaata atattacaat tgtaatattg atgtcaattg
     1141 tttattataa atatttgaaa gaaggtgtca aaatggctaa attactaata atgagcgtag
     1201 taagcttttg ttttatattt ctattgttag tattttttag gtacatatta aaacgctatt
     1261 ttaattatag tttaaattat aaagtttggt atctaactat gcttgctgga ttaattcctt
     1321 ttatcccaat taaattctct tttataaaat ttaataatgt gaataatcaa gcgcccacag
     1381 ttgaaagtaa gtcacacgat ttgaaccata acataaatac caccaaacct attcaagagt
     1441 tcacaacaga tatccataag tttaattggg attcaattga taatatctgc acagttattt
     1501 ggatagtttt agttattatt ttaagtttta aatttttgaa agccttatta tatcttaaat
     1561 atttaaagaa acagtcactt tatctaaacg aaaatgaaaa aaataaaata gatacgatac
     1621 ttttcaacca tcaatataaa aaaaatattg tgattcgaaa agcagaggct attcaatctc
     1681 caataacttt ttggtatggg aaatatatta ttttgattcc tagttcatat tttaaaagtg
     1741 taattgataa aagactaaaa tatataatac tgcatgaata tgctcatgct aaaaatagag
     1801 atactttaca tttaattatc tttaatatct tctctattat tatgagttac aatccattag
     1861 tacatattgt aaaaagaaag ataattcatg acaatgaagt agaagccgat agatttgtcc
     1921 ttaataatat taacaaaaat gaatttaaaa cttatgcgga atctattatg gactccgtat
     1981 taaaaacccc attttccaac aaaaatatat taagccattc atttaatggt aaaaaatcat
     2041 tactcaaaag tagattaatt aatataaaag aagccgatct gaaaaagcag tcaaaattaa
     2101 ttctaatttt tatttgtatt tttacttttt ttatcatgat aattcaaagc cagtttttaa
     2161 tggggcaatc cttaactgat tataattata aaaagccctt acaaagcgat taccaaattt
     2221 tagatgaaag taaaaatttt ggttcaaata gtggatcttt cgttatgtac agcatgaaga
     2281 aagacaaata ctatatttat aatgaaaaag aaagtagaaa aagatattct cctgattcaa
     2341 cttacaaaat ttatttagca ctgtttggac ttgaccgtca tattataagt gataaaaatt
     2401 cacgtatgtc atggaatcat aaacattatc ttttcgagtc ttggaataag gaacaagatt
     2461 taaatacagc aatgcaaaat tcagttaatt ggtacttcga acgtattagt aatcaaatac
     2521 caaagaacta tactgcggct caactcaagc aattaaatta tggtaatgaa aatttgggaa
     2581 gttataaaag ctattggatg gaagatagtt tgaaaatatc taatcttgaa caagtaatag
     2641 tttttaaaaa tatgatggaa caaaataacc attttagtaa aaaagcaaag aatcaattat
     2701 cttcttcatt attgattaag aaaaatgaaa agtatgaact gtatgggaaa acaggtacag
     2761 gtatagtaaa cgggaagtat aataatgggt ggtttgtagg ttacgtaatt acaaatcatg
     2821 ataagtatta ttttgctaca catttatcag atggaaaccc gtctgggaaa aatgctgaat
     2881 taataagtga aaaaatatta aaaggaatgg gtgttttaaa tgaccaataa gcaagttgaa
     2941 atatctatgg ctgaatggga tgttatgaat ataatatggg ataaaaaatc agtatcagct
     3001 aatgaaattg tagttgaaat tcaaaaatat aaagaagtta gtgataaaac gattagaaca
     3061 ttaatcacaa gactatataa aaaagagatt ataaaacgat acaaatcaga aaatatttat
     3121 ttttactcat caaatattaa agaagacgat attaaaatga aaactgctaa aacctttctt
     3181 aataaactgt atggagggga tatgaaaagt ttagtgctga attttgcgaa aaatgaagaa
     3241 ttaaataaca aagaaattga agaattgcga gacattttaa atgatattag taaaaagtaa
     3301 ttagaggtat gtttcaaaca taatacatat gtaaaccata cttgactctt attaaattag
     3361 ttttgttaat gacatttaaa atatattatt aaagatgaga gtgtccagaa aataaccatt
     3421 ttctgtacac tcttgttttt tgtctaaata agtaagtatt acatagaatt atgtgttcaa
     3481 aaacccaaca aaaaatctat gttcacaaaa gacctcgtaa tcaagttatg gtacactaaa
     3541 ggtgtatata ggaggtctgt attttgaaaa taggttatgc aagagtttca actggattac
     3601 aaaatttgaa tttacaggag gatcgattaa atacatatgg ttgtgaaaag atatttaatg
     3661 accatatgag tggttcaaaa agtaagcgac ctggtttaga taaagcaatt gaatttgcga
     3721 ggtcaggaga tacgattgtt gtttggagat tagatagact aggacggaac atggaggatt
     3781 taatcacgtt agttaatgaa cttaacgaac gaggtgtgag ttttcatagt ttagaagaaa
     3841 atataacgat ggataaatca agttctacag gacaattatt atttcattta ttcgcagcat
     3901 ttgcagagtt tgaacgtaat ttaattttag agcgatcatc agccggtcga attgcagcac
     3961 gtgctagagg ccgatatgga ggtagacctg aaaaattaaa taaacaagat ttaaacttac
     4021 ttaagacact ttatgataat ggtacgccaa ttaaaacaat tgcagagcag tggcaagttt
     4081 cacgtacaac tatttatcgt tatctcaata aattagaggc aaaagagaat aaaaacaagt
     4141 agagcaacta atctagctta ctataatgaa tctactaaat aattttatat atcattaaca
     4201 tacaaagact ttgattcaat gtcactcgat gtaaaagaga cgttctgaca gcgtctaaaa
     4261 tctcataata ttttcctgtt atgagatttt aataataaca caagaaaaaa gcgtacaaat
     4321 tcgtacgctt ttttaatata atacctgttt cactaatgaa cgttttaagt atcttcatca
     4381 ttaactgaaa ctacagtaat aatactcgtc aacaatatta gtatagttaa aatagaaaga
     4441 cttaggactg gcataaccga actaaaatta tatttgaaat ataaaagtgc taatgaagag
     4501 agaaatgtaa aaataataaa taccaaagac catttttcta atttattcat tggcttcacc
     4561 tctttgaact tttatataat cataggagat atttttacta cgatttataa aaaataagaa
     4621 gataatcatt attaaagaaa cgattaatat aatgttgaat cctatagtat cacctaattc
     4681 gtttacacct ttgcctaata tcggagatac aaagctttgt attaataatg aaagagatgc
     4741 ccatatagta aatgaggttc tgttgcaaag ttgaatttat agtataattt taaca
//
LOCUS       DANRDI010000406         3142 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.guided.226, whole genome shotgun sequence.
ACCESSION   DANRDI010000406 DANRDI010000000
VERSION     DANRDI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.guided.226"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            181..816
                     /locus_tag="PZ377_003015"
                     /pseudo
     CDS             181..816
                     /locus_tag="PZ377_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            813..1259
                     /locus_tag="PZ377_003016"
     CDS             813..1259
                     /locus_tag="PZ377_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDM6359837.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDDKCVGKVKLRKNWNRYAYIEDIAVCKDFRGQGIGSALINISIEWAKHKNL
                     HGLMLETQDNNLIACKFYHNCGFKIGSVDTMLYANFEKAVFWYLRF"
     gene            1352..2146
                     /locus_tag="PZ377_003017"
                     /note="aph(3')-IIIa"
     CDS             1352..2146
                     /locus_tag="PZ377_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDM6359838.1"
                     /translation="MAKMRISPELKKLIEKYHCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2422..2993
                     /locus_tag="PZ377_003018"
                     /pseudo
     CDS             2422..2993
                     /locus_tag="PZ377_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1054 a          501 c          711 g          876 t
ORIGIN      
        1 ttcgccatgc cacgaaatta gcatcatgct agcaagttaa acgaacactg acatgatata
       61 ttagtgatta gctatatttt ttactttgca acagaaccat catttttatt aaaatactct
      121 tttgtaaaat ctttctgctc ttcaatgtat ttaatttctt tacctctaaa tctattatag
      181 ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga gttagataat
      241 tacctaaagg gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg
      301 gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat
      361 gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag
      421 gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg
      481 atatcatgga aggtcggcat cgaaacaggc tttaaatgaa gtgtaggcaa gaactataag
      541 tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg
      601 gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta
      661 tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca
      721 aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca
      781 tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc
      841 tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
      901 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
      961 accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac cggtacgctt
     1021 atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc agcgcgctta
     1081 tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg cttgaaaccc
     1141 aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa atcggctccg
     1201 tcgatactat gttatacgcc aactttgaaa aagctgtttt ctggtattta aggttttaga
     1261 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     1321 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     1381 gaaaaaactg atcgaaaaat accactgcgt aaaagatacg gaaggaatgt ctcctgctaa
     1441 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     1501 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     1561 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     1621 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     1681 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     1741 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     1801 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     1861 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1921 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1981 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     2041 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     2101 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     2161 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     2221 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
     2281 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
     2341 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
     2401 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
     2461 ctgcaatcaa aaaaataatt cctgccaaag aaattgcaca gcgatttaat ataagcttaa
     2521 ggacagttta cagagatatc cggacacttg aagaagccgg aataccaatt ggatctgagg
     2581 ccggaaaagg atattttctt gtagagggct tcctacttct gccggtaatg tttacagcgg
     2641 cggaagtggg tgcattgatt acagcaggga aatttttaaa ttgccatgga gatgaatcat
     2701 ttataaagga ttttgattca gctatgtata aaatcaaatc tattttgaag catggtgaaa
     2761 aaaactatgc acaggagttg gagaacagta ttaacgtgta cagcacatct ggacagaaaa
     2821 atacattggc ggataacgtt attgcagcaa ttcagactgc aatctgcaat aaaagggtaa
     2881 tatcaattca atatcctgca tcaggagggc aagaaccgga aagcagaatg atagagccta
     2941 tatcactggg gttttatgaa cagaactggt acttaatcgg ttttgcaggc taagaaatga
     3001 atataggaat tttcgtgtgg atcggattaa aagcatttgc atagaagaag agctctgtca
     3061 aagccatgat ggctctttgg aacaaatatt aaaacaaatg ctggttctgt tgcaaagtaa
     3121 aaaaatatag ctaaccacta at
//
LOCUS       DANRDI010000407         2742 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.guided.245, whole genome shotgun sequence.
ACCESSION   DANRDI010000407 DANRDI010000000
VERSION     DANRDI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.guided.245"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            91..975
                     /locus_tag="PZ377_003019"
     CDS             91..975
                     /locus_tag="PZ377_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDM6359839.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            1097..1516
                     /locus_tag="PZ377_003020"
     CDS             1097..1516
                     /locus_tag="PZ377_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDM6359840.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(1772..2707)
                     /locus_tag="PZ377_003021"
     CDS             complement(1772..2707)
                     /locus_tag="PZ377_003021"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDM6359841.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
BASE COUNT          993 a          490 c          381 g          878 t
ORIGIN      
        1 tattcattat agtatgttaa aatatagcca acaaatataa ataaactaat tatccatagc
       61 ttgaatctat aaataaaagg agcaaaacac atgaaaatta ttcagttaga atacttcttg
      121 gctatcgtga aatataatag ttttactaaa gctgcacaat ttttacatat tagccagcca
      181 tctttaactg ctacgattaa aaaaatggaa gcagatttag gttatgactt atttacacgt
      241 tcaacaaaag acatcaagat taccgaaaaa ggaatacagt tttatcgtta tgcgagcgaa
      301 ttagttcaac aatatcgatc cacgatggaa aaaatgtatg atttaagcgt tacatcagaa
      361 ccaaggataa aaattgggac tcttgaatct acgaatcaat ggattgcgaa tttaattcga
      421 aagcaccatt ccgactaccc tgaacagcaa tatcgtttat atgaaataca tgataaacat
      481 caatctatag agcaattact gaattttaat attcatttag ctataacaaa tgaaaaaata
      541 acccacgaag atataagatc cattccttta tatgaggaat cttacatttt attagcaccc
      601 aaggaaacat ttaaaaatca aaattgggta gatgttgaaa atttgccact catattacca
      661 aacaaaaatt ctcaagtgcg caaacactta gatgactatt ttaatagaag aaatattcgt
      721 ccaaatgtcg ttgtagaaac agatcgattc gaatcagcag ttggatttgt tcatctcggc
      781 ttaggttacg ctatcattcc gagattttat taccaatcat ttcacacgtc taatttagaa
      841 tataaaaaaa ttcgtccaaa cttaggccga aaaatttata tcaattacca taaaaaacgc
      901 aaacactccg aacaagtaca tacattcgta caacaatgcc aagattattt atatggactt
      961 ttagaggctc tttaacttaa gttattagag cctcttatgc agttgcgcag atcatcgtat
     1021 aaaaattaat gacgtcattt caaaaatcga tacaaaaata atttattata aaaattctaa
     1081 gaaagaagtg aagcagatgt taaaatctat taatcatata tgcttttcag tcagaaattt
     1141 aaacgattca atacattttt atagagatat tttacttggg aaattgctat tgactggtaa
     1201 aaaaactgct tattttgagc ttgcaggcct atggattgct ttaaatgaag aaaaagatat
     1261 accacgtaat gaaattcact tttcatatac acatatagct ttcactatag atgacagcga
     1321 atttaaatat tggcatcaga ggttaaaaga taataacgtg aatattttag aaggaagagt
     1381 tagagatatt agagatagac aatcaattta ctttaccgac cctgatggtc ataagctaga
     1441 attacatact ggcacacttg agaacagatt aaattattat aaagaggcta aaccacatat
     1501 gacattttac aaataaggtg tcattataaa aaggcctctt gaactccgtt aaaattttaa
     1561 ttaattatta tataataaga gaacttttca aacaatacag ttgttatttt tgctatttca
     1621 acaaacataa ataagcagta agatgactac aacttaagag tcttcttact gcaattattt
     1681 ttcaaatata tcaacgttaa tataacttct attaagaaat actcacattc tgccctgcaa
     1741 tgcaaatctc gtcacatata aatattttta attattttaa aaaatgatgc actaaattag
     1801 caacgagctt agcagttcta ttgtcagcgt catatgttgg attcatctca gcaatactaa
     1861 ctgaagacac cttatcactt ggaataatac gttttgctaa ttcaagaaca gtatgtggat
     1921 acaaacctaa cactgccggc gcacttaccc caggcgcaaa cgcactatca atgacatcca
     1981 tacaaatcgt aaacataatg acatcatgtt catgtacaaa acgttcaatc atatctttaa
     2041 ttgttggtga tacgtgactc aataattcat ctgcaaagac ataatcaatc tttttctctt
     2101 tagcataatc aaataaactt tgcgtattac caccttgagc aataccaagc actaaataat
     2161 ctgtgttttc atcttcttct aaaatttgtc taaagctcgt tccagatgta gattgttgtt
     2221 cagcacgtgt atcaaaatgc gcatcaatat ttatcacacc aatagattgt gttggataga
     2281 ctttacgtgt tgctaaatat tgagcatacg caatatcatg tccaccacct aataaaaatg
     2341 tttgtctatg attagcaatt gacttcgctg caagcatagc aaattctttt tgagtatcaa
     2401 ttaattcctc atgatcatga taaacatttc cgtaatcgac taaagtttca cattgattca
     2461 aatccggcaa acctgcaaat gcttgtttaa tcgcatctgg tccttctttt gcaccaatgc
     2521 gccccttgtt taaagcaaca cctttgtcaa cagcatagcc taatataccg acccctgatg
     2581 gcatactact cttttccagc ttagacaaat cttcaaatgt tactgtttga aaatgtctaa
     2641 attttttcgg gtctgtttca ctatctaacc ttccagtcca taaatttggt tcaccttgct
     2701 tgtacacagc atttccccct cttatttatg tggcttatta ac
//
LOCUS       DANRDI010000408         2670 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.guided.249, whole genome shotgun sequence.
ACCESSION   DANRDI010000408 DANRDI010000000
VERSION     DANRDI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.guided.249"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            154..2163
                     /gene="mecA"
                     /locus_tag="PZ377_003022"
     CDS             154..2163
                     /gene="mecA"
                     /locus_tag="PZ377_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDM6359842.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NDNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(2209..2637)
                     /locus_tag="PZ377_003023"
     CDS             complement(2209..2637)
                     /locus_tag="PZ377_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDM6359843.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
BASE COUNT         1062 a          381 c          424 g          803 t
ORIGIN      
        1 tacacatatc gtgagcaatg aactgattat acttaacatt aaaaaagatg ataacacctt
       61 ctacacctcc atatcacaaa aattataaca ttattttgac ataaatacta catttgtaat
      121 atactacaaa tgtagtctta tataaggagt atattgatga aaaagataaa aattgttcca
      181 cttattttaa tagttgtagt tgtcgggttt ggtatatatt tttatgcttc aaaagataaa
      241 gaaattaata atactattga tgcaattgaa gataaaaatt tcaaacaagt ttataaagat
      301 agcagttata tttctaaaag cgataatggt gaagtagaaa tgactgaacg tccgataaaa
      361 atatataata gtttaggcgt taaagatata aacattcagg atcgtaaaat aaaaaaagta
      421 tctaaaaata aaaaacgagt agatgctcaa tataaaatta aaacaaacta cggtaacatt
      481 gatcgcaacg ttcaatttaa ttttgttaaa gaagatggta tgtggaagtt agattgggat
      541 catagcgtca ttattccagg aatgcagaaa gaccaaagca tacatattga aaatttaaaa
      601 tcagaacgtg gtaaaatttt agaccgaaac aatgtggaat tggccaatac aggaacagca
      661 tatgagatag gcatcgttcc aaagaatgta tctaaaaaag attataaagc aatcgctaaa
      721 gaactaagta tttctgaaga ctatatcaaa caacaaatgg atcaaaattg ggtacaagat
      781 gataccttcg ttccacttaa aaccgttaaa aaaatggatg aatatttaag tgatttcgca
      841 aaaaaatttc atcttacaac taatgaaaca gaaagtcgta actatcctct aggaaaagcg
      901 acttcacatc tattaggtta tgttggtccc attaactctg aagaattaaa acaaaaagaa
      961 tataaaggct ataaagatga tgcagttatt ggtaaaaagg gactcgaaaa actttacgat
     1021 aaaaagctcc aacatgaaga tggctatcgt gtcacaatcg ttgacgataa tagcaataca
     1081 atcgcacata cattaataga gaaaaagaaa aaagatggca aagatattca actaactatt
     1141 gatgctaaag ttcaaaagag tatttataac aacatgaaaa atgattatgg ctcaggtact
     1201 gctatccacc ctcaaacagg tgaattatta gcacttgtaa gcacaccttc atatgacgtc
     1261 tatccattta tgtatggcat gagtaacgaa gaatataata aattaaccga agataaaaaa
     1321 gaacctctgc tcaacaagtt ccagattaca acttcaccag gttcaactca aaaaatatta
     1381 acagcaatga ttgggttaaa taacaaaaca ttagacgata aaacaagtta taaaatcgat
     1441 ggtaaaggtt ggcaaaaaga taaatcttgg ggtggttaca acgttacaag atatgaagtg
     1501 gtaaatgata atatcgactt aaaacaagca atagaatcat cagataacat tttctttgct
     1561 agagtagcac tcgaattagg cagtaagaaa tttgaaaaag gcatgaaaaa actaggtgtt
     1621 ggtgaagata taccaagtga ttatccattt tataatgctc aaatttcaaa caaaaattta
     1681 gataatgaaa tattattagc tgattcaggt tacggacaag gtgaaatact gattaaccca
     1741 gtacagatcc tttcaatcta tagcgcatta gaaaataatg gcaatattaa cgcacctcac
     1801 ttattaaaag acacgaaaaa caaagtttgg aagaaaaata ttatttccaa agaaaatatc
     1861 aatctattaa ctgatggtat gcaacaagtc gtaaataaaa cacataaaga agatatttat
     1921 agatcttatg caaacttaat tggcaaatcc ggtactgcag aactcaaaat gaaacaagga
     1981 gaaactggca gacaaattgg gtggtttata tcatatgata aagataatcc aaacatgatg
     2041 atggctatta atgttaaaga tgtacaagat aaaggaatgg ctagctacaa tgccaaaatc
     2101 tcaggtaaag tgtatgatga gctatatgag aacggtaata aaaaatacga tatagatgaa
     2161 taacaaaaca gtgaagcaat ccgtaacgat ggttgcttca ctgttttatt atgaattatt
     2221 aataagtgct gttacttctc ccttaaatac aatttcttca ttttcattgt atgttgaaag
     2281 tgacactgta acgagtccat tttctttttt tatggatttc ttatttgtaa tttcagcgat
     2341 aacgtacaat gtattacctg ggtatacagg tttaataaat ttaacgttat tcatttgtgt
     2401 tcctgctaca acttcttctc cgtatttacc ttcttctacc cataatttaa atgatattga
     2461 aagtgtatgc atgccagatg caatgatacc tttaaatcta ctttgttctg ctttttcttt
     2521 atctatatgc atatattgag gatcaaaagt tgttgcaaat tggataattt cttcttctgt
     2581 aatatgaagg ctttttgttt tgaatgtttc tcctactata aaatcatcgt atttcatata
     2641 tgtctctctt tcttattcaa aacaaaaagc
//
LOCUS       DANRDI010000409         1758 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.guided.278, whole genome shotgun sequence.
ACCESSION   DANRDI010000409 DANRDI010000000
VERSION     DANRDI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.guided.278"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..258
                     /locus_tag="PZ377_003024"
     CDS             <1..258
                     /locus_tag="PZ377_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDM6359844.1"
                     /translation="HHVGNVLSSDIFYNADTTASERWMRMGILGVEMESAALYMNAIY
                     AGVEALGVFTVSDHLIHETSTTPEERERAFTDMIEIALSLV"
     gene            265..1617
                     /gene="tet(38)"
                     /locus_tag="PZ377_003025"
     CDS             265..1617
                     /gene="tet(38)"
                     /locus_tag="PZ377_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDM6359845.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT          547 a          258 c          317 g          636 t
ORIGIN      
        1 catcatgtag gtaatgtgtt atcaagtgat attttctata acgcggatac aacagcgagt
       61 gaacgttgga tgcgtatggg tattttaggt gtagaaatgg aatcagctgc attatacatg
      121 aatgcaattt acgctggtgt cgaagcatta ggtgtgttca cagtgagcga tcatttaatt
      181 catgaaacgt caacaacacc tgaggaaagg gaacgtgcat ttacagatat gattgaaatt
      241 gcactgtcat tggtgtagat gattatgaat gttgaatatt ctaaaataaa gaaagcagta
      301 cctattttat tattcttatt tgtattcagt ttggttatag acaactcatt taaattgatt
      361 tctgtagcca ttgctgatga cttaaacata tctgtaacga cagtaagttg gcaagcgaca
      421 ttagccggtt tagtaattgg tattggcgct gtagtatacg cttcattatc tgatgccatt
      481 agtatacgca cactatttat ttatggcgtg atattaatca ttatcggatc aattattggt
      541 tacattttcc aacatcaatt cccattactt ttagttggac gtattattca aactgccggt
      601 ttagctgctg cagagacatt atatgtgata tatgttgcaa agtatctttc taaagaggac
      661 cagaagactt accttggctt aagtacgagc agttattcct tgtcattagt tatcggtaca
      721 ttatcaggtg gatttatttc tacgtattta cactggacaa atatgttttt aattgcatta
      781 atcgtagtat ttacgttgcc attcctattt aaattattac caaaagaaaa taatacgaat
      841 aaagctcatt tagattttgt tggcttaatt ctagtggcaa ctattgctac aacagtcatg
      901 ctgtttatta cgaactttaa ttggttatat atgattggtg ccttaattgc gattatcgtt
      961 tttgcgctat atattaaaaa tgcgcaacgt ccattagtaa ataaatcatt tttccaaaat
     1021 aaacgttatg cttcattttt atttatagta tttgtaatgt atgctatcca attgggttat
     1081 atttttacgt tcccattcat aatggagcaa atttatcatc tgcaactaga cacaacatca
     1141 ctgttattag taccgggtta tatagtagca gtcattgttg gtgcactaag tggtaaaatc
     1201 ggcgaatatc tgaattcaaa acaagcgatt atcacagcaa ttattttaat agcactgagc
     1261 ttgattttac ctgcatttgc agtaggtaat cacatttcaa tcttcgtcat ttctatgata
     1321 ttctttgcag gtagctttgc tttaatgtat gcacctttac ttaacgaagc cattaaaaca
     1381 atagatctta atatgacagg tgtggctatt ggtttttata atttaattat taatgtggcg
     1441 gtatctgtag gtattgcgat tgctgcggct ctaatcgatt ttaaagcatt aaatttccca
     1501 ggcaatgatg cattaagttc acatttcggt attattttaa ttattttagg tttaatgagt
     1561 attgtcggat tagttttatt cgtcagctta aatcgttgga cacaatctga aaaataaata
     1621 gatataaatt cgcgagatat attcgtattt atagtaaaat taaataaaga gattatataa
     1681 cacgaggagt agtaagtatg aaatttgaga aatatataga tcacacttta ttgaagcctg
     1741 agtcaacacg tacgcaaa
//
LOCUS       DANRDI010000410         1319 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.guided.297, whole genome shotgun sequence.
ACCESSION   DANRDI010000410 DANRDI010000000
VERSION     DANRDI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.guided.297"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            <1..913
                     /locus_tag="PZ377_003026"
     CDS             <1..913
                     /locus_tag="PZ377_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HDM6359846.1"
                     /translation="LYIKYTQNIMSHKIWLLVLVSTLIPLIPFYKISNFTFSKDMMNR
                     NVSDTTSSVSHMLDGQQSSVTKDLAINVNQFETSNITYMILLIWVFGSLLCLFYMIKA
                     FRQIDVIKSSSLESSYLNERLKVCQSKMQFYKKHITISYSSNIDNPMVFGLVKSQIVL
                     PTVVVETMNDKEIEYIILHELSHVKSHDLIFNQLYVVFKMIFWFNPALYISKTMMDND
                     CEKVCDRNVLKILNRHEHIRYGESILKCSILKSQHINNVAAQYLLGFNSNIKERVKYI
                     ALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            885..1145
                     /locus_tag="PZ377_003027"
     CDS             885..1145
                     /locus_tag="PZ377_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDM6359847.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPYLQKMAVEQVVEQAELMASQQ"
     gene            complement(1136..>1319)
                     /locus_tag="PZ377_003028"
     CDS             complement(1136..>1319)
                     /locus_tag="PZ377_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDM6359848.1"
                     /translation="RELGFVLMALNIRKVVAQRAENNQKIYKKDNFYIISIEIVFFHL
                     SKNFMSRTHYDLFFYC"
BASE COUNT          480 a          184 c          216 g          439 t
ORIGIN      
        1 gctctatata aaatatactc aaaatattat gtcacataag atttggttat tagtgctcgt
       61 ctccacgtta attccattaa taccatttta caaaatatcg aattttacat tttcaaaaga
      121 tatgatgaat cgaaatgtat ctgacacgac ttcttcggtt agtcatatgt tagatggtca
      181 acaatcatct gttacgaaag acttagcaat taatgttaat cagtttgaga cctcaaatat
      241 aacgtatatg attcttttga tatgggtatt tggtagtttg ttgtgcttat tttatatgat
      301 taaggcattc cgacaaattg atgttattaa aagttcgtca ttggaatcgt catatcttaa
      361 tgaacgactt aaagtatgtc aaagtaagat gcagttctac aaaaagcata taacaattag
      421 ttatagttca aacattgata atccgatggt atttggttta gtgaaatccc aaattgtact
      481 accaactgtc gtagtcgaaa ccatgaatga caaagaaatt gaatatatta ttctacatga
      541 actatcacat gtgaaaagtc atgacttaat attcaaccag ctttatgttg tttttaaaat
      601 gatattctgg tttaatcctg cactatatat aagtaaaaca atgatggaca atgactgtga
      661 aaaagtatgt gatagaaacg ttttaaaaat tttgaatcgc catgaacata tacgttatgg
      721 tgaatcgata ttaaaatgct ctattttaaa atctcagcac ataaataatg tggcagcaca
      781 atatttacta ggttttaatt caaatattaa agaacgtgtt aagtatattg cactttatga
      841 ttcaatgcct aaacctaatc gaaacaagcg tattgttgcg tatattgtat gtagtatatc
      901 gagcttcaca tgaaacagct aaagaagctt tgggcgataa agagttaaga gccattgcac
      961 atgagttaac taaaacagtt aaggataaca tgagtgttga ttggtctaaa cgagacagtg
     1021 ctaaagctaa aatgagagtt caagttagac gcctattaaa gaaatatggc tatccaccat
     1081 atcttcaaaa aatggctgtg gaacaagttg tagagcaagc agaattaatg gcaagtcagc
     1141 aataaaaaaa taaatcataa tgagtccggg acataaagtt cttggataag tgaaaaaaga
     1201 caatttctat tgaaataata tagaaattgt cttttttata aattttttga ttattttcag
     1261 ctcgttgagc tactactttt cttatattaa gtgccattaa tacaaaacca agttctctt
//
LOCUS       DANRDI010000411          547 bp    DNA     linear   BCT 03-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain S0078-6M-OS1-C1-C4-CL
            SAMN32801309-rid17285793.guided.352, whole genome shotgun sequence.
ACCESSION   DANRDI010000411 DANRDI010000000
VERSION     DANRDI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32801309
            Sequence Read Archive: SRR23267505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001643068.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001643068.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/25/2023 10:11:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 19
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 10
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 29 of 55
            Pseudo Genes (incomplete)         :: 24 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32801309-rid17285793.guided.352"
                     /strain="S0078-6M-OS1-C1-C4-CL"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA: Pennsylvania"
                     /collection_date="2020-06"
     gene            36..515
                     /locus_tag="PZ377_003029"
     CDS             36..515
                     /locus_tag="PZ377_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDM6359849.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
BASE COUNT          194 a           84 c          103 g          166 t
ORIGIN      
        1 ataaaaaatg aaatgataga agaaggagga taattatgac tttatccatt ctagttgcac
       61 atgacttgca acgagtaatt ggttttgaaa atcaattacc ttggcaccta ccaaatgatt
      121 tgaagcatgt taaaaaatta tcaacaggtc atactttagt aatgggtcgt aagacatttg
      181 aatcgattgg taaaccacta ccgaatcgtc gaaatgttgt acttacttca gatacaagtt
      241 tcaacgtaga gggcgttgat gtaattcact ctattgaaga tatttaccaa ctaccgggcc
      301 atgttttcat atttggaggg caaacattat ttgaagaaat gattgataaa gtggacgaca
      361 tgtatattac tgttattgaa ggtaaattcc gtggtgatac gttctttcca ccttatacat
      421 ttgaagactg ggaagttgcc tcttcagttg aaggtaaact agatgagaaa aatacaattc
      481 cacatacctt tctacattta attcgtaaaa aataaggggg aaaacgacca tgacaaaaca
      541 gattata
//
